LOCUS       DAEVGI010000001        98682 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEVGI010000001 DAEVGI010000000
VERSION     DAEVGI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 98682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98682
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.001"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(122..3376)
                     /locus_tag="K8M77_000001"
     CDS             complement(122..3376)
                     /locus_tag="K8M77_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBI7084533.1"
                     /translation="MLVEDLLLQRFKEETTGRNYAKGQRIINNDLVESVEIEDEEDLV
                     AVNGIVISEQLFSKYSTKLEIDIRSRGILSTYCTCPDYEKHEFSKENYCCKHLVATFY
                     KALGDLAKKKEFEEDKNLFSKKKENKILDLILQEETDKEELKIEVYINKNEWSNSILA
                     EFKIGTKSMSSNKLYILKDIEQFLVAYYNRIPIKYSKAFTFDIREQKLSTKDRELIEF
                     IESLKEIEKPIKSFLRNRDTFIDGKSIKIPNYLVRDFFRVIKNHRIYLNEGFFYRAVE
                     SEVIFEDVPIDFSLKDSKNNFELSSLNGMPKVLGDRADVFLYGTNIYIPKKDFCYKIK
                     NYLDIFSEGKVIKIDKCEEERVFRKLIPSLKDLTENVVLSKNIMNKTVLEPVKFNFYF
                     DKEGKEATLTLKVKYGSYEFNIFDDCKEKIIYRDIKKEKEVIALIERFSLEEINKKFY
                     FYLGDEYIFSFFKYDVCKLQEYGEVYYSENFKGIKSLGSKGIKGDIKPGRYNYFEFDF
                     KIGDIPSEETREILRAFRENLKFFKLKSGEFLDLEELELKQFLKLLDSVDNGDLEKNC
                     LEINNSRALYIANYIEEKGIRYIKGKKKLNELKSKFKNINKLSFEVPKDLKAELREYQ
                     KFGYNWLKTLEHLGLGGILGDEMGLGKTLQAITFILSNKGKKTLVVAPTSLIYNWKEE
                     FNKFAPNLVVEVLNEGKDKREEALRDIESKDVIITTYNLLKRDLEEYKKINFDYCFID
                     EAQAIKNPDSQNSEAVKEVNSEYKFALTGTPMENSLMELWSIFDFVMPGYLYDRKRFT
                     VRYYKKLNESEEVLNEIHSLIKPFILRRKKKDVIKELPDKIEKMHLVDLGEEQKKVYS
                     IYANNALSIMEKKQDAEEFNKSKIEILSYITKLRQLALDPAVTINDYMGESAKIEALV
                     EILNQGIEEGHKILVFSQFTSVLKNISSRLKEEKISFSYLDGSVSSKRRINMVNEFNE
                     GENSVFLISLKAGGTGLNLTSADIVIHFDPWWNPAVENQATDRAHRMGQKNVVEVIKL
                     IAKGTIEEKVVALQEEKKELISKIIEEGELGVGSTFNTLSEEELMDLFKVDYLG"
     gene            complement(3554..4555)
                     /locus_tag="K8M77_000002"
     CDS             complement(3554..4555)
                     /locus_tag="K8M77_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7084534.1"
                     /translation="MGKTTLLDIAKAVNVSKTTVSMVLNNKEINVSKETREKIFKAAK
                     DMNYIPNSLARSLSTKKSYTLGMIIPDIQNPFFSEMAKAIEIESEKHGYSIILCNTLN
                     EKKKEEEYLKLLISKLVDGVIIAACGDGKEWIKILKNNKIPFVMIDRMVSDKKSANGV
                     FCDNKKGVELGVNYLVNRGNKHIAFVTGKANLEIANLRLEGFKNTAKNLGISNETIIE
                     ESDFSMEGGINATERIIKNNKKVDAIFYSSDVMAIGGMKYLIRNGYKIPEDISILGYD
                     NINICSYMEPELTTIAQPIYKIGESSCKLLMDLINNKSLKSKIINLEPSLVERGTVK"
     gene            complement(4695..5600)
                     /gene="rbsB"
                     /locus_tag="K8M77_000003"
     CDS             complement(4695..5600)
                     /gene="rbsB"
                     /locus_tag="K8M77_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBI7084535.1"
                     /translation="MNINKKIFSVGLMLMMLMTSFIGCGRDNKPKIGMVLSTLNNPFF
                     VNMKDGAEKEAEKLGYDLVVLDSQNDPAKERANVEDLIQLGVIALLINPTDSDAVVKT
                     VEVANKSNIPVITLDRQANGGKITSHIASDNIKGGEMAAEYVLDKFKDEKGPINVVEI
                     QGIPGASATRDRGEGFHNIMDKNDKFNFISIQAADFDRQKGLQVMENIIQANPNIQVV
                     FAHNDEMALGAVKAIKASGINALVIGFDGNDDAKDSIDANEMTATIAQQPDLIGALGV
                     ELANKIYKGESIKNKIAADLKVYTK"
     gene            complement(5622..6557)
                     /locus_tag="K8M77_000004"
     CDS             complement(5622..6557)
                     /locus_tag="K8M77_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBI7084536.1"
                     /translation="MKANVKANISKYKSLIGLVLLCIVITIVTPNFLSVSNITNVFTQ
                     VSVNAIIAIGMTFVILTGGIDLSVGSTLAISGAIGASIVKSTGNVFLAIIVAAVIGIA
                     VGLINGLLVSKGKLQAFIVTLATMTIFRGATLVFTDGTPISKLPEVFVKIGNGKLGFM
                     PIPVIITIIIAIIAVYALSQTRFGRYLYALGGNEDASRLSGINTDKIKTLVYVVSGFA
                     SAIAGVIITSRIGSASPNAGTGFELDAIAAVVIGGTSLAGGEGTITGTLIGALIIGVL
                     NNGLNLMNVSPFYQSIVKGLVILIAVLLDKKSRKN"
     gene            complement(6571..8076)
                     /locus_tag="K8M77_000005"
     CDS             complement(6571..8076)
                     /locus_tag="K8M77_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI7084537.1"
                     /translation="MGERTPMLKMVGVSKSFPGVKALDNVSLMAYGGEVTALMGENGA
                     GKSTLMKILSGVYKKDEGKIFIEGREVEVKGIKSAEEAGITIIHQELSVLNNLTVSEN
                     IFLGNEKHSKFTGRINKKLLDERSKMFLEQIGCDIDPNRLVSTLNVGEKQMIEIAKAL
                     TKNARIIIIDEPTTALTDVETENLFKVIENLRKKGIAIIYISHRMEEIFKICHRVEVL
                     RDGKYAGSAEIKDIDNDKLIAMMVGRTIEDQFPYRDVKKGDLALEVKNLSCKEGVKGA
                     SFTLRKGEILGIAGLMGSGRTELAKTIFGEYKKTSGEISLNGSLININCISDAINNGI
                     CYLSEDRKKEGCILGMSVGENMTLCNLKKYENKFKSLDKKEEAKDIEYYIKKINIKTP
                     NKEQFIKNLSGGNQQKVILAKWLMLSPEVLIIDEPTRGIDVGAKKEIYELLNELKASG
                     KVIIMISSDLPEVLGISDRIMVMSEGRISGELNRDEANQESIMKLAVGINN"
     gene            complement(8163..8558)
                     /gene="rbsD"
                     /locus_tag="K8M77_000006"
     CDS             complement(8163..8558)
                     /gene="rbsD"
                     /locus_tag="K8M77_000006"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBI7084538.1"
                     /translation="MRKTSLLNSNISSVISKMGHTDMLAIGDCGLPIPKETERIDLAL
                     IKGVPGFIETLKAILEELQVEEVLIAKETEKVSPELFTEIKEIIKDTKITFISHKELK
                     KELKDCKAVVRTGEQTPYANIILKSGVVF"
     gene            complement(8560..9492)
                     /gene="rbsK"
                     /locus_tag="K8M77_000007"
     CDS             complement(8560..9492)
                     /gene="rbsK"
                     /locus_tag="K8M77_000007"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI7084539.1"
                     /translation="MNKICVLGSMNMDLVLKVKDMPKVGETILSKSFQKIAGGKGANQ
                     AVAAKRSGAEVFMISKIGKDENGRELRDKLVEDNIDVKYVFEDRIEPTGMALIMVNDN
                     GNNSIIVNAGSNMTLTKEEIHSAENLIKESDIIISQFETPEDITIEAFKIAKENGKVT
                     ILNPAPAKKIKDQLLKYTDIIVPNETEAELLTGIEIKDIEDAKKAGDIFLGKGVKFAI
                     ITLGEKGAALIGKDFCEIVPAYRVNAIDTTAAGDSFIGGLSSKLDTKNLGRETLSSSI
                     RFGNKVSSIAVQRKGAQPSIPYLKEVLEVYKGEE"
     gene            complement(9632..10099)
                     /gene="def"
                     /locus_tag="K8M77_000008"
     CDS             complement(9632..10099)
                     /gene="def"
                     /locus_tag="K8M77_000008"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI7084540.1"
                     /translation="MAVKKIVQIGHEALKKVSEPVKDVNEVKGLIQDLKDTLATVEGI
                     GLAAPQIAVNKRVVYINFGDGENEYVLINPEVIGVSKETYEDYEGCLSYVMHEGLVER
                     PRAVRIQALNEKGELKVYEAQDLLARCFLHEIDHLEGIMYVDRAKEMYELVEK"
     gene            complement(10255..11214)
                     /locus_tag="K8M77_000009"
     CDS             complement(10255..11214)
                     /locus_tag="K8M77_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBI7084541.1"
                     /translation="MIKYLIVMLIPFVIVYLLMPKFIEFATKKGFVDKPTGRKKHDKL
                     TPLIGGVVMYIGFFATYLFGIDSTRQLSQTFIIFIASTLVIIIGVIDDYYKGKGKEFP
                     IYPRVIIQILAATMVFKAGIVFRGITNPFTGEFTQFSGWIQYILTITWIFGVTTVINW
                     SDGMDGLAGSISIISAMTMFVVALAKGQFNSAYMSITLVGSILAFLRYNRHPARVFMG
                     DSGANFLGFILAIIALEGAFKQATALSILVPVLALGVPIFDNIFVIFRRFQSGKPVYE
                     ADRSQIHYRLQERGMSVKQIVSYICILSGCLSLVSLLIIMIIL"
     gene            complement(11360..11926)
                     /locus_tag="K8M77_000010"
     CDS             complement(11360..11926)
                     /locus_tag="K8M77_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7084542.1"
                     /translation="MNSVMNLTTLEQIKAYSDPYRLKIITYLRNNQESATVKEIADFF
                     GEVPAKVHYHIKKLEKAGILELVKTKEIKGIIAKYYYLTAESFNIEGNQIKKEAKQVY
                     KSQILNILSEYYDSSKEQTMKRISEKVDTGNAEDSIGFNYKNIYLNEEEFKEVNRQMR
                     ELVKKYERKEEGKMPCHVFSVCLKHEVE"
     gene            complement(12122..12766)
                     /locus_tag="K8M77_000011"
     CDS             complement(12122..12766)
                     /locus_tag="K8M77_000011"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBI7084543.1"
                     /translation="MAQRLNVNPTRMELSKLKKRLATATRGHKLLKDKQDELMRQFIN
                     LIKYNNQLRDEVEKELGSSLKDFVMARAVMSSEFLEEAIAYPKEEIEVEVGNKNIMSV
                     NVPIMNFKRKLEGDEGSIYPYGFMNTSAELDDAISKLYGILPKLLELAEVEKSGQLMA
                     DEIEKTRRRVNALEYMTIPQLKETIRYIRMKLDENERSALTRLMKVKSMIEQRG"
     gene            complement(12769..14151)
                     /locus_tag="K8M77_000012"
     CDS             complement(12769..14151)
                     /locus_tag="K8M77_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBI7084544.1"
                     /translation="MLKEYRTVTEVVGPLMAVEGVEGVKYDELVEIEMQTGELRRGKV
                     LEVNGDKAMVQLFEGSAGINLKNTKVRFLGRPLEIGVSEDMLGRVFDGMGRPKDNGPN
                     IIPEKRLDINGEAINPVARNYPSEFIQTGISAIDGLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQAKVLNSDSKFAVVFAAIGITFEEAEFFVDEFTKTGAIDRSVLFMNLASDPA
                     IERIATPRMALTTAEYLAYEKGMHVLVIMTDITNYCEALREVSAARKEVPGRRGYPGY
                     LYTDLSTLYERAGRLVGKEGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKGIMPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFSAYAQGKQAKELAAILGES
                     ALSDVDKAYAKFAEAFENEYVSQGFTTNRTIEETLNLGWKLLKILPRTELKRIRDEYL
                     EKYMPVGEDE"
     gene            complement(14144..15919)
                     /locus_tag="K8M77_000013"
     CDS             complement(14144..15919)
                     /locus_tag="K8M77_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBI7084545.1"
                     /translation="MKTGKIIKVSGPLVVAEGMDEANVYDVVKVGEKGLIGEIIEMRG
                     DKASIQVYEETSGIGPGDPVITTGEPLSVELGPGLIESMFDGIQRPLDAFMKAANSAF
                     LSKGVEVKSLNREKKWPFVPTAKVGDKVSAGDVIGTVQETAVVLHRIMVPFGVEGTIK
                     EIKAGDFNVEEVMAVVETEKGDKNLTLMQKWPVRKGRPYARKLNPVEPMTTGQRVIDT
                     FFPVAKGGAAAVPGPFGAGKTVVQHQVAKWGDTEIVVYVGCGERGNEMTDVLNEFPEL
                     KDPKTGESLMKRTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVSIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLADYYERAGKVVALGKDGREGAVTAIGAVSP
                     PGGDISEPVTQSTLRIVKVFWGLDAQLAYKRHFPSINWLTSYSLYLEKMGEWMDAHVA
                     DDWSALRTEAMALLQEEANLEEIVRLVGMDALSEGDRLKLEVAKSIREDYLQQNAFHE
                     NDTYTSLNKQYKMLNLILSFKHEAERALEAGVYLDKVLKLPVRDRIARSKYISEEEIS
                     KMDDILVELKSEMNKLISEGGVLNA"
     gene            complement(15936..16253)
                     /locus_tag="K8M77_000014"
     CDS             complement(15936..16253)
                     /locus_tag="K8M77_000014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBI7084546.1"
                     /translation="MDMYKKIGVVGDKDSVLAFKALGIDVFPVVEDEEARKTIDKLAM
                     TGYAVIFVTEHVAKNIEETIERYTRQVLPAVILIPSNQGTLNIGKQRISDNVEKAVGV
                     NIL"
     gene            complement(16240..17247)
                     /locus_tag="K8M77_000015"
     CDS             complement(16240..17247)
                     /locus_tag="K8M77_000015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBI7084547.1"
                     /translation="MDSMQFLQLIPRIRVYEKKLLDKAQIERMIDANTSEGALKVLQE
                     TEYAAFMNEVRRPDDYEILLSNELVRLYKTMYEASPLKEIIDVMALKYDYHNIKVLVK
                     GKILDKDFSQMLIPVGMVGVNDLKSAINGEDFGRLPKKMEECLRKIFEKFEETNDPQF
                     IDLIADQYLYAHIHEIANKKNIKDKALDRYLTNLIDLNNVKTLLRAKKQDKNVNFFLD
                     AIVPGGSIDASKFRSLYLDTIENIPNNLSSKEYSQVLREGIASYIETSSVSEFEKLID
                     NHIMSMMKEAKIVTKGLEPVLTYMYVKENEIKQIRTIMVGKLNNITKEVIRERLRDGY
                     V"
     gene            complement(17260..17856)
                     /locus_tag="K8M77_000016"
     CDS             complement(17260..17856)
                     /locus_tag="K8M77_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBI7084548.1"
                     /translation="MSNLNNLTSKILNDAEEKKKYILAEAEAQKDKIISKKTNRAEAD
                     KEEIITKANIEAEVKKARIISNAKLSVRNDMLRAKQDVISKVFNEAIEKLQNLSNGDY
                     KYYVISTLDSLELEGTEVIIINEKDKDIFSNEFLEALNKELESKGKKGSITLNIEGKF
                     NGGFILDRNGIQINNTFEALINSLRGELEFEVNKVLFD"
     gene            complement(17884..18378)
                     /locus_tag="K8M77_000017"
     CDS             complement(17884..18378)
                     /locus_tag="K8M77_000017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBI7084549.1"
                     /translation="MENWISYFMANYGGLIFGALGIALAVGMSGIGSAKGVGIVGEAA
                     AGLVTEEPEKFGKALVLELLPGTQGLYGFVIGFLVFNQISNGDASLAKGLYLLFACLP
                     IAIAGLWSGISQGKAAAAGIQILAKRPEHNTKGIIFAAMVETYALLGFVISFLLVNGA
                     SVIK"
     gene            complement(18433..20379)
                     /locus_tag="K8M77_000018"
     CDS             complement(18433..20379)
                     /locus_tag="K8M77_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBI7084550.1"
                     /translation="MAIVKMNKFTLLAFESEKEKLLKKIQSSSEVEFINLQDEEKVEG
                     NEVFESLNKDDLDAETTLIEENVSKTRSALDFLKEFVAEVGGLKALKAGKESLTLDEL
                     EVKVENSNWEVVYSEVKKMERELATLENEKTKLLGEIEILEPWQSFDAPLGELNNFEK
                     VVAFLGVISSQNLENMKNEIESEFKESYIEVISNTDQDSYVFVMTMKERAEEMDEVLK
                     NFGFSAFQTKYKEKASVLVEEFKLKIQEIEAKKSDLKGVLSNYREEKRTLELAYEYYS
                     NILLRKEASENFLKTDRVVVIQGWVPKNDNSSLEGIIQSSVGDMYYLEFEEVKEEEVA
                     EVPVKLHNKGPAAAFDSITEMYSLPRYDEIDPTPLLTPFYLIFFGMMVADLGYGLVLF
                     VGSLLAMKLLNLDEAQEKFAKFFMYLSIATTIAGAIFGTAFGFELKFGLINPSKDTNL
                     LLILSVGFGVIQIFFGLFIKAYMLIRDKQYLYALFDVGSWIMLLIGLPMIFFDGTISL
                     VGKVLSIVGSILIILTQGRDEETKGAQIGQGLYALYGITGYVGDLVSYTRLMALGIAG
                     GSIAAALNLIIGMFPGIAVIIVGPLFFIAAHTFNMLLSLLGAYVHTARLQYVEYFSKF
                     YEGGGKAFTPFRTINKFITIKRNK"
     gene            complement(20366..20692)
                     /gene="ahaH"
                     /locus_tag="K8M77_000019"
     CDS             complement(20366..20692)
                     /gene="ahaH"
                     /locus_tag="K8M77_000019"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase archaeal subunit H"
                     /protein_id="HBI7084551.1"
                     /translation="MAIEAIKEIKKVELQADEMIKKAHEQSKKIISDATIEADERYNS
                     IIEEAKNVARGIISNAEEAGRKEAEVILSEGEKKCAEVSSLKGSKIDSAVNLVIERIV
                     KTNGNS"
     gene            complement(20969..22648)
                     /locus_tag="K8M77_000020"
     CDS             complement(20969..22648)
                     /locus_tag="K8M77_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HBI7084552.1"
                     /translation="MKILLTAINSKYIHSNLAVRYLKAFTKDLDFQGDIKEFSINDRV
                     ENILEGIIEEKPDVVAFSCYIWNMEFVNRLAELIKLVDPNIEILYGGPEVSYEGKEFL
                     ENHEGEYVIVGEGEKTFREFVLYKLGEGKIEDIKGLNYKRDEKVFENPKRPEMDMNEL
                     VFPYTYEEDINNKIVYYEASRGCPFKCKYCLSSVMHGVRFLDVERVKKELKYFMERGL
                     KLVKFVDRTFNCNREYTVELLKYLSEQDTETRFHFEVAADLLTEEQIEILNNAPKGRF
                     QLEVGVQTTNNEVLHNINRYITYENIKEKVLKVAAGKNVMQHLDLIAGLPGEDLESFK
                     KSFNDVHNIRPDEIQLGFLKLLKGSSMREEAKKWGIVYSPYAPYEIIRSKDISYEELL
                     LLKKVEAMVDKYYNSCKFNNVVKFFLNIYEKPFDFYYDLAIFFEEKGNFKRSIGNVEY
                     YKILLDFYLEKIGGKDEGLFKEVLKFDYLCFNKKRWLPDFLVRTITKEDEQNIKDSFD
                     RQMPFKKAHIEKFEIDIINYIKNGEINFEPKYLIFDEMNFDYDSYVEVEKK"
     gene            complement(22548..23150)
                     /locus_tag="K8M77_000021"
     CDS             complement(22548..23150)
                     /locus_tag="K8M77_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084553.1"
                     /translation="MVREVILDMSNINFKGNTLDVGINNYGIIYNILKQTESEISVDY
                     YVENFNGEYRNQYDNGVLFFSLSQLHKNEVEDVVKNIWSNVRFGGNLYIWDREKQKKE
                     TVNDKVKVLIGGNKEKEFEFIDNSISNEFTVDSSKKILEKYFEIEETKVCDKIIFIKG
                     IKRGSIKNENTANSNKLKVHSQQPSSEVPEGIYKGFRFPR"
     gene            complement(23297..24583)
                     /locus_tag="K8M77_000022"
     CDS             complement(23297..24583)
                     /locus_tag="K8M77_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HBI7084554.1"
                     /translation="MKNFIKFISLILISALLGSIICFFTNKDGKYLESESFSEELILK
                     GLKGARALCNDEENIYIALENKILKIDRNNNVFLELKEEGNIYDLEYYNKFLYYTLDE
                     KLVSYNIKSSEREVLIEDIPNKGINKEDTRILINDGKLYLTIGTSTNSGIVDKEGENP
                     DIPPVDIVLSGRNYDENKKGAFAPYNTKTVKGEKVKGNILGNGAIIEFDIESKKKQLY
                     SYGIRNVKGFDLNSSGEIFAVVGGMEDEGVRPLSGDSDYIYKIEGKGTWYGWPDYSGG
                     DPVNSPRFREEGKPIINFVTDAHKSYVMPKPLYQSEDTRNINTLLIDKKGIILEDENS
                     FLFFNNKNNTLLKLLKEGEVKELIYLDKNSYINDMKIIGKNLYILDGNKGVLFRLEKS
                     NTINNIPIYNYFVILGINFILIGVLGIKFLLSLKKK"
     gene            24730..26259
                     /gene="spoVB"
                     /locus_tag="K8M77_000023"
     CDS             24730..26259
                     /gene="spoVB"
                     /locus_tag="K8M77_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein B"
                     /protein_id="HBI7084555.1"
                     /translation="MSKNDFYKNSFMLTASNLTTGLLGFIFSMYLSKVLGPEGMGLYG
                     IIMPIYNLFISIMTAGIIASISKITAIYSARDDYKNIIRTMKVVAIFNFIWCLIIGIF
                     VFFLSPIIGHFWAKDPRIIKSIMITCPAMIFIALSNILKGFFYGTSKITVPSFIDILE
                     KSLRIFVLAILIFIFKAETLESLVTLAYLALCLGELQSLILLFGYFKYSMSKFPKTNA
                     KGESRAQLLFDVLVTSVPLCLNGFLMSIFSVISTLLVPKRLIVAGFTYSQALSLIGKY
                     SSMAMSIVTFPIIIVSSINTMLIPDLSQTLSKGNYLSATKRIRDVIKIAFLIGICTTV
                     IGLCVPDSLGKLFFGRDDLGQYIRITSVMMPIVFTSNTMYGILNGLGRQNVILRNTII
                     TEVLEVTLLFFLTAIPSINIYGYAITMLIISSLSLCLNLYEIYKNINIGLSLSNFLIY
                     ILTGVLTYICLSPLSLKLSFIDFRVQVLAVTSIAASIFIFLIIKEKFSSRFKKISLKS
                     R"
     gene            complement(26353..27153)
                     /locus_tag="K8M77_000024"
     CDS             complement(26353..27153)
                     /locus_tag="K8M77_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084556.1"
                     /translation="MRKIKVNGIIFLISLILCLFLGGNTALAKENPNTIEFLSYDEEI
                     LILRSNGETVLISEGDNVERIIEGINKENLKEIDLLVLRSMEEETDELIKTFINKSSV
                     KKILLPTLNGVSEEVSNLTKEKNIEIASMEEGFNYNKESISLNAKKVENSKEGMIFAT
                     VDNIKLAVLSEEAYDSAINLSNIEDCRVEILNLIDSEKENKKEVKKLVKRLKPLVIID
                     DLDNGKSIEKLGVKDYNLNEEKGLKIMRVLGENKEFKVLSKKEGYTSK"
     gene            complement(27264..28106)
                     /locus_tag="K8M77_000025"
     CDS             complement(27264..28106)
                     /locus_tag="K8M77_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBI7084557.1"
                     /translation="MIINIGQFNKMKVVREADFGYYLDRGTRNNSDDILIPTGNLDGR
                     EIKVGDEVEAFIYRDSRDRLIATLKKPLAVAGDVALLEVKSITKIGAFVDFGLERDIL
                     VPLKEQKYKMEEGKKYLVYIYVDKTGRLAATTEVDKYLDEIEEPKMGTEVSGIVYGYQ
                     TNGSLRVAIDNKHRAVVLKNEYFTNIKPGDNIKGKIIKVYEDGIVGLSLRNHKLKERN
                     ELGDTILNYLKENGGVMSLNDKSTPDEIRNTFKTSKNYFKMALGGLMKKGLIEQDEFG
                     TRLK"
     gene            complement(28118..28621)
                     /locus_tag="K8M77_000026"
     CDS             complement(28118..28621)
                     /locus_tag="K8M77_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBI7084558.1"
                     /translation="MLIEIRGKKPEIGEKTFIAYSSDIIGDVTIGRDCGVWFGSVIRG
                     DYNLIKIGNETNVQDNAVLHGDKEYKVEIGHGVTIGHGAIIHGCKIEDECLIGMGAII
                     LNGAKIGKNTMIAAGTLVSQNKEIPEGVLVMGVPGKVVRKLTEDEIESIKNSRREYVK
                     MKNLYIK"
     gene            28884..29885
                     /locus_tag="K8M77_000027"
     CDS             28884..29885
                     /locus_tag="K8M77_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HBI7084559.1"
                     /translation="MDKKSIPFDKLKFYYGIPHSHTSLSTGKSSPYESLEHARSNGLD
                     FLIITDHNKYLSETIKEKNREISKWEYLNKCINKFNKKHSDFLALCGFEAKSTTLGHL
                     NILCPNTFFTGIIPDIKYLLLWLINDHTALLSINHPKNSIAKKIEFTPILDKLLCSIE
                     VGNGIHPSTYTRYDSQFFSLLDKGFKIGAINSQDNHKLNQGDSENLTCVISHKLNKNS
                     LLDAFKNRHIFSTESKTLKMLFSLNSTFMGGTITVYSASKISLYLQVEDNINKISKVQ
                     FLTNLGKIIKEIKDINLHNVKYIFEKEVSLNETWFVAKVYLNNNKEAMSSPIFVNYE"
     gene            complement(29913..30245)
                     /locus_tag="K8M77_000028"
     CDS             complement(29913..30245)
                     /locus_tag="K8M77_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084560.1"
                     /translation="MGIFVLILQIILLAVVIFGGFSLLSRFVFNKVKINKWIILVAAI
                     IIFLIPMFIHMNQWIMLAISAVATILFLWFLDILRNGYPKLKKEKKVVIKPKAKPNRV
                     KHNKDSKK"
     gene            30483..30953
                     /locus_tag="K8M77_000029"
     CDS             30483..30953
                     /locus_tag="K8M77_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HBI7084561.1"
                     /translation="MSLINTSTHLDELDLQILDILIKDCRTPYLEIARICHVSGGTIH
                     VRMKKMEDLGIIKGTRILLNLPKLGYDVTCFVGIHVNSTSSYTHVFDELSKIKEVVEI
                     HLITGGYSIFAKVICKNISDLQDILMNKINHIEGISGTDTFISLSQPIDRNITL"
     gene            31061..31264
                     /locus_tag="K8M77_000030"
     CDS             31061..31264
                     /locus_tag="K8M77_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBI7084562.1"
                     /translation="MKTLNIIALTLVIIGALNWGLIGIMNFNLVDSIFGIASIWSRTI
                     YVLVGLAGLYCLSFYGYFNRIKE"
     gene            complement(31312..33057)
                     /locus_tag="K8M77_000031"
     CDS             complement(31312..33057)
                     /locus_tag="K8M77_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HBI7084563.1"
                     /translation="MRKEFCLKHGKVTMNFTAKYCDSFESIINSEGFIKVLETYLKKT
                     KNKKSHNFRFLSEAIGTEDIKVISRYLISALKLLSMMGADEVIVVNDAFEGLLEDKKS
                     FADIIEDVYSFWRKLERYTVIQNNKIKDGIAAVGFIDANKNFNDLILRFYRRLQKNLL
                     GSMPNILRQVSAGGNASIMVSNLIWPSSREYSILEHIPFIDAISLEAPFITYPSKNTR
                     DGIFLETSENPLSGSHINSEEWFCYPAKVGELLAYVYFHRDFMSHGISLCNLFDLATV
                     EECRGVKPDIIYIFGAKDDDDELKTCFYDDEKNNIMLGYANYSEEIDYFGYMKKMILT
                     LHNIIMIKRGYMPIHGAMVNVVLKNGKEANIVIMGDSGAGKSESLEAFRALSEEYISD
                     MTIIFDDMGVFKNVDGIIKGYGTEIGAFVRLDDLDQGYAFKEIDRSIFMNPDKINARL
                     LMPVCKYDDIIRGYDVDLFLYANNYDGLDEGEKSIEYFNNPEEAKKIFKAGARMAKGT
                     TTENGLVESYFANPFGPVQKKEEMDLIIDKYFEDMFNNKVKVGQIKTCLGVLGLEKEG
                     PKKAAIELFNIIEKM"
     gene            33256..34110
                     /locus_tag="K8M77_000032"
     CDS             33256..34110
                     /locus_tag="K8M77_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI7084564.1"
                     /translation="MKITSKKVSIFLIFILLIWIFLYSTSYKILNENFTVHYIDVGQG
                     DSALIQSNGKNLLIDAGTPESTKPLVKYLKNLGIKKLDFIIATHPHADHIGGMESIIR
                     KFDVDYFCAPKVTENTDVFKGMVTALKEKDININTISKSKSLPIDLGENTKIEVYSPS
                     DNKYENLNNYSPILKIYYGETSFLFTGDAENEAEMEAVKSNKNLKSDVLKVGHHGSKT
                     SSTDSFLKEVSPKIAVISSGLDNSYGHPAKETLKKLSSYKTKIFRTDEDGTIILTSDG
                     KSIKKHRN"
     gene            complement(34167..34850)
                     /locus_tag="K8M77_000033"
     CDS             complement(34167..34850)
                     /locus_tag="K8M77_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HBI7084565.1"
                     /translation="MIIKEHEIDINDLDLSNALSFNENYDIYKQAVYFDLEHYVYKKP
                     KCIGVFGAGYFDEEKSKFITTQYMIEHKGEVRDILYLAKKYFIMVAAEKKKKYIVTFS
                     GNNDFTVINYLFDKYGINFDFNNYFRSVDIQKIYEHTFKQGIGLKALEKKFGIEREGD
                     VLSGSNLAKTFHKVLKDKDYFQRMPKEKVEKILVYNEQDVVNLFYILLSWKKILVNLE
                     DSESKESKN"
     gene            35036..35254
                     /locus_tag="K8M77_000034"
     CDS             35036..35254
                     /locus_tag="K8M77_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBI7084566.1"
                     /translation="MSIYDLKIGQVGIVDKICGHDGLAKRLLALGCIEGTEVKLKRVA
                     PLGDPLIVNLRGFDLAIRKKDAKNIILK"
     gene            35292..37055
                     /gene="feoB"
                     /locus_tag="K8M77_000035"
     CDS             35292..37055
                     /gene="feoB"
                     /locus_tag="K8M77_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBI7084567.1"
                     /translation="MTTIALLGNPNVGKTTLFNALTGSNQHVGNWPGVTVDKKEGFFN
                     SNKIVDLPGIYALDTFSNEEKVSKKFLESGDVDLILNIVDASNLDRNLYLTMQLKDFK
                     KPIVLLLNMNDIAEKKGIKIDPKKLEKAFNLKVIPITASKKEGLDEVKEFLNKGNFEK
                     YLDKNTYSFKDEADTYKFIEDKLSDCFNRSSNSSTTLTDKLDKIFLNPWLAYPLFIAL
                     MYFVFQFTFSWVGQPISDALDGFLGDTFMPYLSNLLSGTAPWFQSLLVDGIVAGVGGI
                     IVLLPIILALFLCIAILEDSGYMARVAFLMDKFMRKIGLSGKAFLPMITGFGCTVPAI
                     MSARTLESEKDRKLTALLAPFMSCNARLPVYVIFAGLFFPDNASMVVASLYILGILVA
                     FILGLVFKNTLFKKDEEPFIIELPEYKVPEVRSVLAQLWDKSKSFLKKAGTIIFAMSV
                     VIWFLQSFNFTGMVEDITHSFLYNIGSFIAPIFAPLGFGDWQASVSLLTGLMAKETII
                     STMEVIYKGDLTHMLPQFFTALSSYSFMVFVLLYTPCISVVGVMKKEYGGKFTILSVI
                     FQFLVAWISAFIIYHIGLLIF"
     gene            37070..37219
                     /locus_tag="K8M77_000036"
     CDS             37070..37219
                     /locus_tag="K8M77_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBI7084568.1"
                     /translation="MEILITALIVIAVVFILYKNFKKSSKGQCSGCSGCASKGSCPSR
                     KDNKK"
     gene            complement(37265..38962)
                     /locus_tag="K8M77_000037"
     CDS             complement(37265..38962)
                     /locus_tag="K8M77_000037"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBI7084569.1"
                     /translation="MDYKKLVAERIKEYVDLELENIEKLIEIPPKPEMGDFAFPCFQL
                     AKVMRKAPNMIAAELAEKINKEGFERVECLGPYLNFFVDKVAFSKNIISKVLEEGDKY
                     GSSKIGEGKNVVVEYSSPNIAKPFHVGHLFTTAIGHSLYRMLNFEGYNPIRINHLGDW
                     GTQFGKLISAYKRWGNEEALEEAPINELLRIYVKFHDEAENNPELEDEGRMYFKKLED
                     GDQEAVALWERFKDLSLKEFNKIYDMLGVDFDSWAGESFYNDKMDKVVEELEKANILT
                     ESNGAKVVMLDEYNMPPCIVVKSDGASIYATRDLAAASYRHKTYNFDKCIYVVGKDQI
                     LHFNQVFKTLELAGNEWAKNCVHIPFGLVKFADRKLSTRKGNVVLLEDLLNEAIDKTR
                     ETIEEKNPQLENKEEVAKKIGIGAILFTYLKNSRERDIVFDWKEMLSFDGETGPYVQY
                     SYARAKSILRKAEEQEITAEPDFTKLTSKEEFELAKTLEGLQKAVILGIDKLEPSVVT
                     RYSIEVAKAFNKFYNNHTVLNVEDEGLKAARLELIKATAQVIKNALFLIGIDVVEKM"
     misc_binding    complement(39064..39270)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            39444..40952
                     /locus_tag="K8M77_000038"
     CDS             39444..40952
                     /locus_tag="K8M77_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBI7084570.1"
                     /translation="MDRIGVIDIGSNSVRLTLAEVEKSGYFKIIDELKEHIRLGSDFF
                     ACSVLDEEKIKLTISSIKAFKSMCISCGASKIILIGSESIKNVDEKINIKEIIKKELG
                     LDLRILSNEEEIHYNFLGAKNSLYIDNSLLLDINGNNTHLCWLKNNEIYKEVTLPFGY
                     VTLTSLFQLNDMIGYENNTNCVNYIKEHLKKIDWLKDNSFKSVIGVGGSFRNLGKIDR
                     RRKRYPIDIVHNYVLYDYDIDEMFHLLKSKNLRQRQKIEGLSPSRADVIVASTDLIKE
                     VLSFTNTNEVIICGRGLREGLIYEYIIDKFHTYDDILDISLKGIMDTLNINIPHAQNV
                     CKLAFSLFEGLKSLHHLGDEFKNILKTASLLHDCGISIRYYDHHVHSFYIILNSHISG
                     LTHKEILLSALCAAYHRNNSFQAPIAKYSTIINRLDVKHVEYLGVILKLAEGLDRGLT
                     GHVENVDVIINEDTVNLKLYSEYDLELELRQARRSSKVFEEVYNKNLVIEKA"
     gene            complement(41050..42264)
                     /locus_tag="K8M77_000039"
     CDS             complement(41050..42264)
                     /locus_tag="K8M77_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBI7084571.1"
                     /translation="MIRMSKKIKVILASLLIVCSVLVINSIISNNVVTTKGASIDEKE
                     VIKENDKKGFFNRFSLGRKVNISAVGDIILHDEQIWSAYNEENKAYDFMNNFKYVKNF
                     IEKSDIAYGTIEGTYAGEEIGYSGYPNYNGPDSMIDALKDTGFDIINVATDHSLDKGV
                     EGASKTGEKIDKEMTSIGNKKYIIKKVKGIEIGFTSYTYESKEGELNGHKIPEDMNLN
                     TFSYNKLDKELEEMKALVEEMKNEGAEFIVFGMHWGVEYKTEPSKYQVKIAEALNEYG
                     VDLILGSNPHVVQPIEEIEGEDGNKTLVAYSLGNFISNQRLETMGDRRTADGVILNLT
                     LDKSRKGVKIEKWDYTSTWVYKIPRENKKSYYYILPVEETLNSEEGKKLDQETLNQLN
                     KSLEATKSIVEK"
     gene            complement(42429..43049)
                     /locus_tag="K8M77_000040"
     CDS             complement(42429..43049)
                     /locus_tag="K8M77_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBI7084572.1"
                     /translation="MQFLLDAFLSIPAILIAFSVKEYTRAKMADKLGDKSPRFKGELT
                     LDPLKHVDIAGALMMAFFGFGWSKSVEINKYAFKNPKKDALKVNIAAWLSNLVVAIIG
                     VILTSLYLRFFGLRGDLSQIIFLILQYIIILNVNFFVFNILPLPGLDCFRILEDLKPQ
                     LFYKLSGIVYQYYYPILIVIILLGRYVLAIPSQLVMAFVYSIGLFI"
     gene            complement(43254..44633)
                     /locus_tag="K8M77_000041"
     CDS             complement(43254..44633)
                     /locus_tag="K8M77_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBI7084573.1"
                     /translation="MKKTYILDTNVLLYSPGAIYSFEDNNVIIPEVVLEELDNIKKIN
                     NDLGANARHVARELDKLRLSGSLSEGVDLPKGGKLKVVTNFYNTEIPEAWNISKPDNR
                     IIQICKALKEKGEDVCLITKDIFERIKADTVGIKSEDFYEVVVPEFEEQYSGRMEVYT
                     SSECLSKFFKNKVMEKKDLTFYDEENKCYVEPKLEINQFLIIHCNDNDKQTALGRFDG
                     KVIRPLLYKDNNNIMGISPRNVGQKFMLECLSMDAKKAPLVIIKGPAGTAKTLFSLAV
                     GLQKILEEESGQYRRILVCRPNVTMDEEIGYLPGTEQEKIAPFMRPIYDNLEILIDSD
                     EKERYSNERELNDKIEELFERKIITTEAVAYLRGRSIIKNWIIIDEAQNLTPKQVKAI
                     ITRAGEGSKIILVGDPEQIDQAFLDSRSNGLCYASEKMKGSHLCYQVTLKYDECERSE
                     LAYEAAKRL"
     gene            44855..45469
                     /locus_tag="K8M77_000042"
     CDS             44855..45469
                     /locus_tag="K8M77_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBI7084574.1"
                     /translation="MKLLFFDTETTGVKPGSICQLSYILVNTSTKPQKTIGKNIFFTV
                     DEMEPEAERIHGFSLEKLYELSEGMYFEDLYADFINDFLEADFIIGHNVQFDIKFLKH
                     ELLGLGEFFEPNNVFCTMKYYKDICKILRPTGDYKNPKLEEVIKHLNITDSEISETAN
                     KYFQGSGNYHDARFDTAATYLTVINGIKKGLIPRNYFTKLLEAK"
     gene            complement(45506..45835)
                     /locus_tag="K8M77_000043"
     CDS             complement(45506..45835)
                     /locus_tag="K8M77_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI7084575.1"
                     /translation="MNKVDLIGRVATKIEVKESKNNKKYIRFRIAVNSFNGREETTTF
                     LSVITWSKSTVDFLEKFVNIGDLVSVSGEIVESRYESESGEVRYYTNIQTSNINLLNK
                     AKEKEIS"
     gene            complement(46114..47544)
                     /locus_tag="K8M77_000044"
     CDS             complement(46114..47544)
                     /locus_tag="K8M77_000044"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBI7084576.1"
                     /translation="MKNLYSTPLNSRYASKEMSYIFSDDMKFSTWRKLWVALAEGEKE
                     LGLNITDEQIEELKSHISDINYEEAIKKEKEVRHDVMSHVYAYGLQCPSAKGIIHLGA
                     TSCYVGDNTDVIIMRDALLLIKKKIVAVLNNLKRFALEYKDMPTLGFTHFQPAQLTTV
                     GKRATLWMQDLVMDMENIDFLLSTLKLRGVKGTTGTQASFMNLFEGDEEKVKALDKIV
                     AEKMGFKKSFGVTGQTYPRKLDSIILNTLSEIAQSAYKFSNDLRLLQSMKEIEEPFEK
                     NQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITASTQWFERTLDDSANKRIAVA
                     EAFLALDGVLNLYINIAENMVVYDKVIEAHVNQELPFMATENIMMESVKKGGDRQELH
                     ERIRVHSMDAAQRVKGEGLNNDLIKRIINDPSFNLSKEEIIAIIDPVKFVGRAPSQVV
                     EFIDEYVNPIIEANKDAASLSSDITV"
     gene            complement(47787..48047)
                     /locus_tag="K8M77_000045"
     CDS             complement(47787..48047)
                     /locus_tag="K8M77_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBI7084577.1"
                     /translation="MVTKEVIVKNSTGLHARPATLLVKKASAFKSDISLEYNGKKANV
                     KSLIGVLSLGVSKDANVKVIASGDDEALAAEEIAKLIENLEE"
     gene            complement(48142..49335)
                     /locus_tag="K8M77_000046"
     CDS             complement(48142..49335)
                     /locus_tag="K8M77_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBI7084578.1"
                     /translation="MILSRKAQNIGASLTLALTAKAGELKKEGIDVVSFGVGEPDFNT
                     PKNIIEAATRAMEEGKTKYTATSGIVELKEAIARKLHDDNGLNYGTKNIIISTGAKQS
                     LANVFMAILNPGDEVIIPVPYWVSYPELVKLSDGVPVFIETKKENDFKVTYDELKSVL
                     SENTKAIVINSPNNPTGTVYSKKDLEVIAKFAKENDLIIISDEIYEKLIYGKEEHISI
                     ASSSEDAFKRTVVINGFSKAYAMTGWRIGYAACYNEELIKVMNNVQSHMTSNTNSIAQ
                     FAALEALNGDQETIKNMVKEFSLRRELMIELISEIEDLTFIEPKGAFYVMIDVSKVLK
                     KANIKGSMEFANLLLKEENVVVIPGIAFGEDNFIRLSYATSKEEIIKGLKRIKEFVNK
                     LMNRD"
     gene            complement(49487..49747)
                     /locus_tag="K8M77_000047"
     CDS             complement(49487..49747)
                     /locus_tag="K8M77_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBI7084579.1"
                     /translation="MEVLKVSTKSNPNSVAGALAAIIKERNTVEIQAVGAGAINQAVK
                     AIAIARGFVAPSGKDIVCIPAFTDIEIDGEERTAIKLIIQPR"
     gene            complement(49965..51500)
                     /gene="rny"
                     /locus_tag="K8M77_000048"
     CDS             complement(49965..51500)
                     /gene="rny"
                     /locus_tag="K8M77_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBI7084580.1"
                     /translation="MVVGILIGIIILGVVGFIQYTLIEKASKNRVESLEKEASLALEE
                     AKREAESTKKEAILEAKEEVHKLRSDLDKEIRDRRNEIQRFERRLIQREESLDKKGEM
                     LEKREDSINKKSIEIQELEERVQSLYGEQRAELERISNLSSEDARTLLLDEVRREIKH
                     ESAMLIKELETKAKEEADKKSREIITNAIQRCAADHVSETTVHVVALPNDEMKGRIIG
                     REGRNIRTLETLTGVDLIIDDTPEAVILSSFDPIRREVARIALEKLIVDGRIHPARIE
                     EMVERAIKDVENDIKEEGEQATFETGVHGLHPEIIKLLGRLKYRTSYGQNVLKHSIEV
                     SYLAGLMASELGLDVNLARRAGLLHDIGKGVDQEYEGPHAVIGGELAKKYHESPAVVN
                     AIAAHHGDTEMQTLEAVLVQAADAISAARPGARRETLEAYIKRLEKLEEIATSYEGVE
                     KSYAIQAGREIRIMVKPDQVDDAGAIEMARNIVKKIEEQLEYPGQIKINVIRETRAVD
                     YAK"
     gene            complement(51848..52906)
                     /gene="recA"
                     /locus_tag="K8M77_000049"
     CDS             complement(51848..52906)
                     /gene="recA"
                     /locus_tag="K8M77_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBI7084581.1"
                     /translation="MANIDKDKLKAIEMAMGQIEKQFGKGSVMKLGEQGAPQMDAVST
                     GCLDLDIALGIGGVPKGRIIEIYGPESSGKTTVALHVVAEAQKLGGAAAYIDAEHALD
                     PVYAKRLGVNIDDLVVSQPDTGEQALEITEALVRSGAIDVLVVDSVAALVPRAEIEGE
                     MGDSHVGLQARLMSQALRKLTGTINKSNCVVIFINQLREKVGIMFGNPETTPGGRALK
                     FYASVRMDIRRIDSIKQGDGITGNRTRVKIVKNKVAPPFKQAEFDIMYNEGISKEGNI
                     VDVGVKENIVQKSGAWFSYGDIRLGQGRENAKQYLKENPSVALDIENQIREKYSLPLA
                     KAVESTSVEKNTEESVES"
     gene            complement(53042..53632)
                     /gene="pgsA"
                     /locus_tag="K8M77_000050"
     CDS             complement(53042..53632)
                     /gene="pgsA"
                     /locus_tag="K8M77_000050"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBI7084582.1"
                     /translation="MNLANKLTLIRIILVPIFLVFIAYQDLPYGSVIATLIFIIASVT
                     DQLDGYIARSRNQVTNFGKFMDPLADKLLVTAALISLVELQLVPAWAAIVIIAREFAV
                     SGLRTIAASEGLVIAASWWGKIKTVIQIIAIVLLLLQVNIINSPGIKELVSGSSFLRN
                     FFIYVPDIFLYLAVAITIISGVDYFRKNKNVINTYK"
     gene            complement(53616..54953)
                     /gene="rimO"
                     /locus_tag="K8M77_000051"
     CDS             complement(53616..54953)
                     /gene="rimO"
                     /locus_tag="K8M77_000051"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBI7084583.1"
                     /translation="MAKYKVGMVSLGCDKNRVDSEIMLGMVQNEYELTNNPKEADIII
                     VNTCGFIEKAKQESINTILDMAKYKTSHNCKLLIATGCLTQRYGDELLELMPEIDIML
                     GVNDYAKINEAIMNFINGNNEKVKATNYSDVSINEGLRLITTDKATAYLRIAEGCDNF
                     CTYCIIPKIRGKFRSRALESIVEEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHL
                     VLRELAKIEGIEWIRVLYCYPEAIYDELIKEISVNDKVCNYLDLPIQHISNNVLKRMG
                     RKTTKEEIIGKINDLRKNVPNIVLRTSLIVGFPGESCEDFNELKDFIKTIKLDKVGVF
                     TYSREEGTPAAIMEDQIDEEVKKDREEEIMLLQKELSEEINKNKLGREYDVLIEKFNG
                     EYYIGRSYEMAPDIDGCIYVKGNGAKKDQFCKVKIEKALEYDLVGVVCNESCK"
     gene            complement(55022..57412)
                     /locus_tag="K8M77_000052"
     CDS             complement(55022..57412)
                     /locus_tag="K8M77_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBI7084584.1"
                     /translation="MAGGKKKTATKTQKKKNQSQSKVTEEIYALIAICVSLIVMFSLY
                     TDKAGYLSVISRTLLIGLFGIGAFFIPIYIIYLCTKLFLFKREVLFSRIGLGITIALI
                     TSVLLIQTVNINDYYVQGSIWGSIKTIWYSQSEWHGGVVGFLIVLPLYKLVGKIGLFV
                     IFVTLYLISSMLIFDYNLVSIRNFFGGFKEKASKVKFVDKKYEKDDYINLKKKDGVSE
                     GNKESKKDDISDDNNKIKILDFMKNSSLNDIEDNKKEKDNQLKDNIKINDFKQESKMP
                     RDLSLDNTIIEQRGFNSEKAKEEESIDKEISNNIASKCSSVGASYVAPNADLLNLNNN
                     NELDKDDKKALLANAAKLEETLMSFGVEAKILQVTKGPSVTRFELQPKAGIKVSKIVN
                     LADDIALGLAAKGVRIEAPIPGKSAIGIEVPNKEQTPVFFREIVESKEFLDNKFKVAC
                     ALGKDITGKAVVTDLSKMPHVLIAGATGSGKSVCINTLIVSILYKYSPDEVKLLMVDP
                     KVVELNVYNGIPHLLIPVVTDPKKAAAALNWAVNEMTRRYKLFADNGVRNIESYNALY
                     NKGEVPEKLPYIVIIVDELADLMMACPHDVEDYICRLAQMARAAGMHLVIATQRPSVD
                     VITGVIKANIPSRISFAVSSQIDSRTILDSAGAEKLLGRGDMLFYPVGESKPQRVQGA
                     FISEEEVEHVVSFIKESQRDAQYEEDILEHINSATIASEGNGDGDRDELLDEAIEIVV
                     ESGQASASYLQRRLRIGFNRAARIIEELEECGVISRRDGSKPRQVLLSKDELENIK"
     gene            complement(57544..58233)
                     /locus_tag="K8M77_000053"
     CDS             complement(57544..58233)
                     /locus_tag="K8M77_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBI7084585.1"
                     /translation="MEHNKENENKSIEEIKEMGAVLPEHKKEDSNIQVLTIIGQIEGH
                     SVQPPQTKTTKYEHMIPQLIDIEQNDRIKGILFVLNTVGGDVEAGLAIAEMIRSLKKP
                     TVSLVIGGGHSIGVPLATASNYSFISPSATMIIHPIRMNGLVIGVPQTFNYFNKMQER
                     INDFIIRTSEIKLDVLKKMMMQTDELLNDMGTILVGEEAVKCGLINEVGGVKEALDKL
                     NSLIEAYNVEP"
     gene            complement(58287..59483)
                     /gene="dapG"
                     /locus_tag="K8M77_000054"
     CDS             complement(58287..59483)
                     /gene="dapG"
                     /locus_tag="K8M77_000054"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBI7084586.1"
                     /translation="MKIVVQKFGGTSVSTKERRTMVVEKVKGAINKGYLPVVVVSAMG
                     RKGEPYATDTLLGLVSEEFKKENKLATDLLMGCGEIISTVVMSDELREAEIEAVPLTG
                     GNAGILTDDNYSSADFIDINPKLILEVLKEGKVPVVAGFQGVDRNGFLTTLGRGGSDT
                     TAAVLGVALKAEEIEIYTDVDGIMTADPRIVGDAELINKISYNEVFQLADQGAKVIHP
                     RAVEIAMKGNVTLVIKNTMSTCIGTMIDSLGDVDNNKFITGITHQGNRIQVSIKSDDN
                     KDNINYKTILESLANNKISLDLINIFPKEKVFTIDAGVKELFEDIMRKNNLKYSLVED
                     LSTIAIVGSRMRGIPGVMAKIVGALDNENIEVLQTADSHMTIWCLVESKNVREAIKAL
                     HRVFMK"
     gene            complement(59497..59772)
                     /locus_tag="K8M77_000055"
     CDS             complement(59497..59772)
                     /locus_tag="K8M77_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBI7084587.1"
                     /translation="MSNNVRYLSEIERYEIININDGEKYSGLINNDVIIDEEGKLKYL
                     IVNLGGGKFNLFNSDEYLEIEWENVKRIGAKTIILDVDSDMIKKTKL"
     gene            complement(59907..62015)
                     /locus_tag="K8M77_000056"
     CDS             complement(59907..62015)
                     /locus_tag="K8M77_000056"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBI7084588.1"
                     /translation="MNHTHETIIAGRPMKVEFGKLGMLSDAAILMSYGDTVILTNVNA
                     SEKPREGIDFFPLSVEYEERLYSVGKIPGGFIKREGKPSEKAILNGRAIDRPLRPLFP
                     KGYRNDVQVVCTVVSVENDNLPEILAINAASMALCLSSIPFTTPVAAVQVGLIGEEFI
                     LNPTSKEREESSLQLTVCATKERVMMIEAGGDEIPEDTMINAIKFGFDKCQDIIKFQE
                     EAVSMFGKEKKVPELHKVPEEIEEAVREFAFDMISESMHITDRDERNAAMDEVKAKIN
                     EEFEEKYPDNMSDIGEAVYDMQKEVVRHMLLKEGKRPDGRAFDEVRNIGCEVGLLPRT
                     HGTGLFTRGLTQVMTVATLGAISEIQILDGIGEEESKRYMHHYNFPAYSVGEVRPLRG
                     PGRREIGHGALAERALEPLIPSEAEFPYTIRLVSEVLSSNGSTSQASVCGSTLALLDA
                     GVPIKRPAAGIAMGLITSKDLTEEEVLTDIQGLEDFFGDMDFKVAGTEEGITSIQVDT
                     KIKGLSEKVIYDAIYGARKARLMILDKIKECIPAPREEVSKYAPKTSTLQIDPEKIRD
                     VIGAGGKVINKIIADTGVKIDIKEDGLVYVSSAESEGVKEAVKIIEGLTKEVKSGEIY
                     LGKVTKIAQFGAFVEVLPNKEGLVHISKLDNKRVEKVEDIVSVGDEILVKVTEIDSQG
                     RINLSRKDAIKEAEEENKQQ"
     gene            complement(62107..62370)
                     /gene="rpsO"
                     /locus_tag="K8M77_000057"
     CDS             complement(62107..62370)
                     /gene="rpsO"
                     /locus_tag="K8M77_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBI7084589.1"
                     /translation="MDKIRKQEIIAKHARHEGDTGSPEVQIALLTERINSLTDHLRTH
                     KKDHHSRRGLLMMVGQRRGLLNYLYEQDIERYRAIIKELGLRR"
     gene            complement(62486..63415)
                     /locus_tag="K8M77_000058"
     CDS             complement(62486..63415)
                     /locus_tag="K8M77_000058"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBI7084590.1"
                     /translation="MILINNDFKRVVQKSTYVALGSFDGIHKGHLALINKSNELSRKN
                     DSLSMVYTFKNHPRKFINKAGAPKLLVTLHEKIRILEDLKVDLSSFVEFNKKFMELEP
                     EEFIDNLIKNYNVRGIVVGFNYRFGHKNKGDVKLLKELCDLKGLELYIIDPFTYKSEV
                     VSSTRIRKALSEGELEDANNMLGRYFSLNGEVVSGKKIGRTINFPTANLKNNDEIILP
                     KIGVYYTNVEVDGEIYKGITSVGNNPTVNGKNTTVETHILNFDEDIYGKHIKLYFISK
                     IRNEKKFNSLEELKEQLIKDKKFAEESNIKISL"
     gene            complement(63419..64303)
                     /gene="truB"
                     /locus_tag="K8M77_000059"
     CDS             complement(63419..64303)
                     /gene="truB"
                     /locus_tag="K8M77_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBI7084591.1"
                     /translation="MNGVINIYKNTGMTSFDVVAMVRRVAKMKKVGHTGTLDPAASGV
                     LPVCLGKATKIIDYIMENKKVYRVNLKLGMVTDTYDLEGEVLREEDASHITKDEILNC
                     INSFLGTIDQVPPMYSALKQNGVRLYELARQGIEVHREARKVTIYSIENIKIESNDNI
                     QMDVCCSKGTYIRSLCYDIGEKLNVGATMTALERIQNGPFTKEEAINIEDLTEELLEK
                     HIISIEKALDSFEKITVNEKFGKLLRNGVKVFDNRMYSEEVEFNKLYRVYEDNGVFLG
                     LGKRDEKGFKLEKLLIEE"
     gene            complement(64307..65260)
                     /locus_tag="K8M77_000060"
     CDS             complement(64307..65260)
                     /locus_tag="K8M77_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBI7084592.1"
                     /translation="MRFHDVISIINKSKKIGITYHVSPDGDALGSVLALLQGIRIYGK
                     EAYVISKDLVSDNLSFLPYAEEITGEVIKPSEDTDCVIVLDCGNVERISADLSEFKGT
                     LVNMDHHLSNDKYGVINYVDTEASATAEIIFMLLKELKVEINENIAKCLYTSLITDTG
                     SFRYSNSTERTHIIAGELVNYIKHDEIHRNIFDNKPFAKMKLMSLVLGDMELVCNNKI
                     VLMRITKEMIDSIGEEVQNSSDVVSLGNQIKGVEGCILLKEVDEGVKVSLRSKDTLDV
                     RKIAENFGGGGHTKASGAFIKGVSIDEAKNKILEILAKEMV"
     gene            complement(65264..65614)
                     /gene="rbfA"
                     /locus_tag="K8M77_000061"
     CDS             complement(65264..65614)
                     /gene="rbfA"
                     /locus_tag="K8M77_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBI7084593.1"
                     /translation="MANFRGKRINEEVRKEVSDIIRNQIKDPRLTAMVSVTQVEVTKD
                     LRYAKVFVSLFAKNDEEKEESLKALKSSAGFIRREVGNRVKLRSTPEILFEEDNSIDN
                     AMYIESLLNKIKEK"
     gene            complement(65636..67675)
                     /gene="infB"
                     /locus_tag="K8M77_000062"
     CDS             complement(65636..67675)
                     /gene="infB"
                     /locus_tag="K8M77_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBI7084594.1"
                     /translation="MSKIRVYELAKELRVPSKVLINVLMDEFGVEVKNHMSVIEDEDA
                     ALIKELLAGSEANSDLVAEYEAELAEEVNNAAKKKKKRKKGSEDDSLEQDVEVIEIGK
                     TITVKELAEKLNKPVNDVIKTLIFTGVMAAINQEIDFETAEKVAEKYEVAVYEKEEEN
                     TLEEFEEETDVEEENLAKRPPIITVMGHVDHGKTSLLDAIRKSKVTSTEAGGITQHIG
                     AYTVEVNGETLTFLDTPGHEAFTAMRARGAQITDVVILVVAADDGIMPQTVEAINHCK
                     AANVPMIVAINKMDREGANPDRVKQELTEHGLVVEDWGGDIIAVPVSAKTRENIDTLL
                     EMVLLTSEMQELKADAGRKAKGTVVEAKLDKGRGAVATLLVQNGTLHMGDSIIVGSTY
                     GRIRAMFDDSGKKIKSAGPSIPVEVLGLSEVPAAGDRFTVVKDEKTARNMAEARKEKI
                     RQESFATSHRVSLEDLYSQIKEGSVKELSVIVKADVQGSVEAIKASLEKLSTDDVKVR
                     VIHGAVGAISETDITLAAASNAIVIGFNVRPDNNAVAASERDGVEVKTYRVIYDAIED
                     IKSAMIGMLDPEYKEVVLGTAEIRATYKISNVGTIAGGYVLTGKLVRNADVRVIREGI
                     VIFESKLASLKRFKDDVKEVNAGYECGFSVEKFNDIKEGDIIEAYTMEAVQRKEL"
     gene            complement(67706..68008)
                     /locus_tag="K8M77_000063"
     CDS             complement(67706..68008)
                     /locus_tag="K8M77_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7ae-like protein"
                     /protein_id="HBI7084595.1"
                     /translation="MDKFLNFLGLTKRAGKLLEGYNKCEEEMKRSKIYLFIFSNSISN
                     RSKDKFVKYCEQYDIPYIDNFSKEELGYSIGRCEINILGVTDENMAEKLLSYSNNN"
     gene            complement(68001..68267)
                     /locus_tag="K8M77_000064"
     CDS             complement(68001..68267)
                     /locus_tag="K8M77_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBI7084596.1"
                     /translation="MKPKKIPMRMCNGCMEMKPKKELIRIVKNAEGEVSVDLTGKKSG
                     RGAYICKSVECLEKAFKAKRLNRNLQSNIGDEVYDRLRDEITNG"
     gene            complement(68295..69395)
                     /gene="nusA"
                     /locus_tag="K8M77_000065"
     CDS             complement(68295..69395)
                     /gene="nusA"
                     /locus_tag="K8M77_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBI7084597.1"
                     /translation="MNEEFLGALSEIVKEKGISVEALLETIDDAIIAAYKKNFSNSGT
                     TAQNVKVKRDEKSGEIHVYAQKVVVEEVYDDVTEISLEDAKAISAIYQLDDIVEIEVT
                     PKNFGRVAAQLAKQMVIQRIKESERNVIYSEFAEKEFDILPGTVIRKDKGNVFVDLGK
                     IEGVLGPNEQMPTEKYNFNEKLQLYVVEVKKTSKGASVLCSRTHPGLVKRLFELEVPE
                     IYEGIVEIKSIAREAGSRTKIAVYSNDESVDAMGACVGPKGVRVQNIVNELKNEKIDI
                     IKWSNTPSEYIENALSPAKVVSVEADEETKSAKVIVDDSQLSLAIGKEGQNVRLAAKL
                     TGWKIDIKSKSKAEELLQEEDIVVEEDIIIEE"
     gene            complement(69415..69879)
                     /gene="rimP"
                     /locus_tag="K8M77_000066"
     CDS             complement(69415..69879)
                     /gene="rimP"
                     /locus_tag="K8M77_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBI7084598.1"
                     /translation="MKKEQLVADLEALCAPIVKEKGYDLYHIEYVKENNEYYLRLYIE
                     KPEERISLRDCEIVSRALSDMLDIEDPIKDAYFLEVSSPGLNRRLHSDEHFNRFIGKE
                     VFVGFKSSLSGRKNVKGILKDVQENEIIVECEGNEIKVPKDKIKTANLKGEI"
     gene            complement(70112..74461)
                     /locus_tag="K8M77_000067"
     CDS             complement(70112..74461)
                     /locus_tag="K8M77_000067"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBI7084599.1"
                     /translation="MSNEFVKQINRSIKSDDNLNDLEFEITKFQLLKKSNTLRTIIKS
                     KDQLSEEQKKIIKQYIKKAIGFEINIEIMYYIDISDITLKQVVDQHWSHVCEKIIEKH
                     PVLKEVLLNSPIVIEGEKIIIKNGSEFLCTFVNKKHIDREIKGYIKSFFGINSLVEVK
                     YDESLANKNYNDEKLNENKEIAKKVIETMKAQAAQEKPVKKESSDNKHKSNGGNKGGY
                     EKKSYKDEPKNENTILGRNIQGDTIDISSIDMGSGIVTISGDVFKTDIFETKTGRIIL
                     TFFITDYTSSIAVKCFLRDKDKEHVLENVKKGLYCKVRGEATMDPYAKEVVIMARDIN
                     KLTKIERMDTAEEKRVELHMHTTMSSMDAVTAASKIVERAAKFGHKAVAITDHGVVQA
                     FPDAQIAAKKNNIKVIYGVEGYLADNGTPIVINGHEESFDDEYVVFDIETTGFSSKND
                     KIIEIGAVKLKDGEIVDSFSTFVDPKVNIPYKITELTSITQNMVNGQPTIDEVLPKFM
                     EFVGNSVLVAHNAAFDVGFIKKNLMDMGKTLKNPVMDTVPLARYLYPDLKKVKLNLVA
                     KHLGISLENHHRAVDDAKATAEILKFSFKKMKEEMDIHDVKTLNEKYLSNIDVKKLPL
                     HHIIILAKNQTGIKNLYKLVSMAHLDYFARRPRLPKSIITEYREGLIIGSACEAGQLY
                     KAVLEGKTDGELKEIASFYDYLEIQPIQNNEFLIRKGNVKDEEELRELNRKIYDLGKE
                     MDKPVVATCDCHFLDPNDEVFRRIIMAGQGYGDADNQPPLYFRTTNEMMKEFEYLGEE
                     ACREVVIENTQKIADMVEAVKPIPDETFPPKIEGAEEEIRNMTMNKVHSIYGENLPEV
                     VQKRLDKELNSIINNGYAVLYLIAQKLVAKSLEDGYLVGSRGSVGSSFVATMSDITEV
                     NGLPPHYVCPNCKKSEFFLDGSISSGADLPDKNCPDCGAKYIKDGHDIPFETFLGFEG
                     DKEPDIDLNFSGEYQAVVHKYTEVLFGKGYVFKAGTIGTVAEKTAYGFVKKYLQERGL
                     VVSQAEIERLTIGCTGIKRTSGQHPGGIMVVPNDNEIYNFCPIQHPADDVNTDIITTH
                     FDYHSISGRLLKLDILGHDDPTVLRMLQDLTGLDPKTIPLNDPKVISLFTSPDALGVT
                     KEELGCEVGSYGLPEFGTKFVRQMLVDTQPKSFADLVRISGLSHGTDVWLNNAQYFIK
                     EGYTTLKDCIATRDDIMVYLMYKDLPPKKAFTIMEKVRKGKGLSEDDEALMREKNVPD
                     WYIESCKRIKYMFPKGHAVAYVMMAVRIAYYKVYYPEAYYTTYFTVRADDFDADLICK
                     GEEAIKAKMEELNSLGNNISVKEKGLLTILEISYEMYKRGLNFLKVDLYKSEATKFKI
                     EEDGIRPPLNALQGVGDNAAKSIVECRVNGEFISKEDLRIRSKVSKTVIETLDNHGCL
                     EGMQESNQLSLFG"
     gene            complement(74539..75588)
                     /gene="ispG"
                     /locus_tag="K8M77_000068"
                     /gene_synonym="gcpE"
     CDS             complement(74539..75588)
                     /gene="ispG"
                     /locus_tag="K8M77_000068"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBI7084600.1"
                     /translation="MNRKETRKVKVGNIYVGGDFRVSIQSMTNTDTKDVESTVKQIRE
                     LQEAGCDIVRCAVLDMDAACAIKDIVAKINIPLVADIHFDYRLALKAIENGVSAIRIN
                     PGNIGSREKVEAVVKACKEKNIPIRIGVNSGSLSKELLAKYGKPTPDALVESALEHVK
                     ILEELDFHDIVISMKSSNVETMIESYRIASQKTNYPLHLGVTEAGTPWRGTIKSAIGI
                     GTLLAEGIGDTIRVSLTGDPVEEIKVGKEILKNFGYVKEGIEFISCPTCGRTQIDLIN
                     IAKEVEERLSSCKKNIKVAVMGCVVNGPGEAREADIGIAGGKGEGLIFRKGEIIKKVK
                     EEDLVEELIKIIETI"
     gene            complement(75608..76615)
                     /gene="rseP"
                     /locus_tag="K8M77_000069"
     CDS             complement(75608..76615)
                     /gene="rseP"
                     /locus_tag="K8M77_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBI7084601.1"
                     /translation="MYIIFALLAFSALILVHELGHFIVAKLNGIYVEEFAIGMGPKLF
                     GVKVGETEYNLRILPFGGFVKMLGEEDESDDSRSLNAKTPIQRILVMGAGAFMNYVLA
                     LIIFIGLAMSSGFAENKVASVVPNSPAQEIGIKQGDEFLKIDGNKIHTTDDFRMGLAL
                     AKGNPVELEIKRGNDVLTKTVQPILNESGMYQVGISYALVEKPTLLQGIKQGFNETRS
                     LVSQSFIALKTIVTGEANLKTDVGGPVTIIKMSGQAAKAGANTLLWFMAFLSVQLAVF
                     NLLPFPALDGGRIFIELIQMIIRKEIPAKYIEAVNTVGFMLLMGLMVLVTIKDIIFPI
                     L"
     gene            complement(76627..77781)
                     /locus_tag="K8M77_000070"
     CDS             complement(76627..77781)
                     /locus_tag="K8M77_000070"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBI7084602.1"
                     /translation="MKRISILGVTGSIGTQTLDVLRFHKEDFELVGITANRNIELTMD
                     IIKEFSPKYVAINHEESYKKLVDLVKSEGLKCEVICGMEGLVKVATLDEIDIVVTSVV
                     GMIGLKPTVEAIRKGKDIALANKETLVVAGELVMREAKENGVKILPVDSEHSAIFQSL
                     QGNAHNKIDKILLTASGGPFRGFTIEDLKSVTPERALKHPKWNMGQKISIDSSTLMNK
                     GLEVIEAHWLFDCPYKDIEVVVHPQSIIHSMVQYTDGAVIAQLGVPDMKLPIQYALNY
                     PNREGNISEKLDLFKIRELTFEKPDLDTFKCLKLAYEAGEKGKLMPTILNGANEVCVE
                     LFLNKKITYLQIPEIIEECMNTFDYNKEVTLDNLINLDKEVRQYIYEKYN"
     gene            complement(78140..78934)
                     /locus_tag="K8M77_000071"
     CDS             complement(78140..78934)
                     /locus_tag="K8M77_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI7084603.1"
                     /translation="MKSNSRYLGALIMAPFIAFIFLGGIWLKLFTIALSFVGLYEYYK
                     TVKVAKLKPISIGGYILLILYYLTSNNFEMLSFLIIIATFILLCVPILNIEYTFIDVA
                     VTILGFIYVGVFFSFIHLVNIKEGGQYLVWLIFLSSWLCDTAAYYSGRFFGKHKLCPK
                     VSPKKTIEGSIGGILGSTLACGIFGIIISNSLSLIPVYHYFIIGILCGVFCQFGDLVA
                     SSIKRYARIKDYSNLIPGHGGILDRFDSILFAGTIVFFYLTLVLKF"
     gene            complement(78952..79713)
                     /locus_tag="K8M77_000072"
     CDS             complement(78952..79713)
                     /locus_tag="K8M77_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBI7084604.1"
                     /translation="MLDFFSKKGNEDSEEIVLIEDKMPKHIAIIMDGNGRWAKKRGLP
                     RTMGHRAGVKTIKTIVKECDKLGVKYLTLYAFSTENWKRPEDEVNALMKLVVEFIKNE
                     IDELHQNGVRVQTIGDLSRFPEEPKKAILWAMEKTKDNTGVVLSLALNYGGRNEIVRG
                     IKSVIEDVKAGKISIDDINEDMFNDYLYTKGMPDPDLIIRPSGEQRLSNFLLWQCAYS
                     EFWYSQINWPDFNGEDLRKAIFDYQNRDRRFGGIK"
     gene            complement(79778..80335)
                     /gene="frr"
                     /locus_tag="K8M77_000073"
     CDS             complement(79778..80335)
                     /gene="frr"
                     /locus_tag="K8M77_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBI7084605.1"
                     /translation="MIKDVIKKSEEKMNKTINSLNSELATMKAGRANPTMLDRIQVEY
                     YGSMCPLNQVANVSSPEPRLLVITPWEKPMLKEIEKAILKSDLGINPNNDGTIIRLLV
                     PELTEETRKNLVKNVKKVGEQAKVAIRSIRKDANDKVKNFKKEGTITEDEMKKGEDDI
                     QKVTDKFVKEIDTIVAAKEKEIMSI"
     gene            complement(80349..81062)
                     /locus_tag="K8M77_000074"
     CDS             complement(80349..81062)
                     /locus_tag="K8M77_000074"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBI7084606.1"
                     /translation="MGTCKYKRVMLKLSGEALAGENGFGIDFNIAMNIAKAVKELVDM
                     GIEVGAVVGGGNIWRGRSGEGMDRTTADYMGMLATSINALALQDSLEALGVDTRVQTA
                     IEMKEIAEPYIRRRAMRHLEKGRVVIFGAGTGNPYFSTDTAAALRAAEIEADVILLAK
                     KVDGVYDKDPHKYDDAKKYDELSYIEVLEQGLQVMDSTATSLCMDNNIPILVFALDNP
                     ENIKKVVLGENIGTIVSKK"
     gene            complement(81155..82066)
                     /locus_tag="K8M77_000075"
     CDS             complement(81155..82066)
                     /locus_tag="K8M77_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBI7084607.1"
                     /translation="MITAKAVKELRERTGAGMMDCKKALTETNGDMEKAVEVLREKGL
                     AAAAKKAGRVAAEGIVKTYVSEDMKKGSIVEINCETDFVALNEEFVGFAGRVAELVAN
                     SNVNTVEELLAEKLDGDKTVQEVLTELIAKIGENMSVRRFERFSVESGLVQSYIHGGG
                     RIGVMAELACEASSPVLAEVAKDVCMQIAAANPLFLSEADVDQESLEKEKEIYRAQAL
                     NEGKPEHIVDKMVMGRIKKYCKEVCLLDQAWVKDGDKSIAKLLEEKSKEVGSPITITK
                     FVRFERGEGIEKKEENFAEEVAKMGGK"
     gene            complement(82146..82847)
                     /gene="rpsB"
                     /locus_tag="K8M77_000076"
     CDS             complement(82146..82847)
                     /gene="rpsB"
                     /locus_tag="K8M77_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBI7084608.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAPYIFTERNGIYIIDLQ
                     KTVKKAEEAYNFIREVSEAGKDVIFVGTKKQAQEAVKEEAERSNMYFVNHRWLGGMLT
                     NFTTIKTRINRLNKLDEMEQDGTFDVLPKKEVIKLKLEREKLQRNLGGIKELDASNIG
                     AMFVVDPRKEKNAIAEAKILGIPVVAIVDTNCDPEEVDYVIPGNDDAIRAVKLIAGKM
                     ADAIIEGRQGEQLAE"
     gene            complement(83229..84005)
                     /gene="codY"
                     /locus_tag="K8M77_000077"
     CDS             complement(83229..84005)
                     /gene="codY"
                     /locus_tag="K8M77_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBI7084609.1"
                     /translation="MSTLLSKTRRLNKILQKSGTEAIAFGDICQLLSDVMSCNVYLVG
                     RKGRILGYSFSEKFECDIMKEKVVVDRKFPEDYNNKLINIQDTIANIPNKGICVFEGV
                     GECLISKKISTIVPIVGNGDRLGTLLLARFGEEFNDDDLVLAEYSATIVGMELLRARQ
                     GEIEEEARKKAVVQLAIGTLSYSELEAVEHIFSELNGDEGLLVASKIADKVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIKILNEKLMDELKKIK"
     gene            complement(84147..86249)
                     /gene="topA"
                     /locus_tag="K8M77_000078"
     CDS             complement(84147..86249)
                     /gene="topA"
                     /locus_tag="K8M77_000078"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBI7084610.1"
                     /translation="MGQKLVIVESPAKAKTIEKYLGKNYVVEASMGHVRDLPKSQLGV
                     DIENDYNPKYITIRGKGELLSKLRKLAKKSDKIYLATDPDREGEAISWHLANVLKIDE
                     NENCRIEFNEITKDAVKNSIKHPRKINCNLVDAQQARRVLDRLVGYEISPLLWRNVKW
                     GLSAGRVQSAALKLICDREEEIKKFNPEEYWTVDVKLKKGKKSFPVKLTTKNKKKIEI
                     KNKEQADQIIDELKENEYIVSKIKKGTKNKNPLAPFTTSTLQQEASKKLNFMTKKTMS
                     VAQQLYEGVEVKKFGTVGLITYMRTDSVRISKEAQEKALNFIDETYGKEYVPEEPRVY
                     KGKKNIQDAHEAIRPTYVEITPEIAKTNLSNDQYKLYALIWKRFIASQMATCILNTNS
                     LEIKNGDYTLRASGSTIKFDGFMKVYEYISGEEEESVLLPELEENEVLKEESIEGKQH
                     FTQPPARYSEAAFVKLLEEKGIGRPSTYVPTISTLISRKYVDREKKILIPTELGFIVN
                     DILSNYFKQIVDTDFTAEMEVKLDNVEAGKESWTHIVDEFFTPLKEDIEKAEKEISKV
                     IIEDKVSDVPCDKCGRLMVIKHGRFGDFLACPGYPECQNTKPIVEEVDANCPLCGGKI
                     LVKRSKKGNRFYGCSNYPECNFVSWYEPTNEKCPECSSYMVKRYSKSKGEYLQCSDKE
                     CKYEKIIEKNNDENNSEK"
     gene            complement(86294..87376)
                     /gene="dprA"
                     /locus_tag="K8M77_000079"
     CDS             complement(86294..87376)
                     /gene="dprA"
                     /locus_tag="K8M77_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBI7084611.1"
                     /translation="MKEVDLNYKIWLGSLKISNNIKVELLNQLGNEENIYIHRKDIEE
                     TGIKCASKFKNDINIDLNSRVINDINKKDYKIVTYLDKDYPKRLKNIDEPPYILFYKG
                     DLERAEDTNVAIVGSRKCDSYGKRCASLIASGLGKAGVGIVSGGAFGVDTEAHKKCVE
                     ENFYNVAVFGCGINLDYPVYNRELYKKIIKNGCIISEFFPDTPPLRYNFPQRNRIISG
                     LSEAVIVVQAGEKSGSLITANYAINQGKDVFAVPGDVFSSQSKGCNNLIKDGAGIFTN
                     IGDLLNSIHFKIKKEGKCNKDGKKEENSEINQLLLSILENNILHIDDIIRVTNIDTSI
                     IYELLCELQFENKIEVIPGDYYTKII"
     gene            complement(87377..88900)
                     /locus_tag="K8M77_000080"
     CDS             complement(87377..88900)
                     /locus_tag="K8M77_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBI7084612.1"
                     /translation="MSISLKSSTLIGIEGVIVNIEVDISKGLPSFTLVGLADTSVREA
                     KERVRSAILNSGYEFPLGRITVNLAPGNLRKVGTGFDLPIALAILIESGQIQREILEN
                     TVYLGELSLDGHIRSVNGALCSVISGVENNNDKFIIPIDNLRECSCVDNINLYGFSNL
                     KEVVGFLTYRDVLPYKREEEICALKKDNSFNEICGQESAKRAMIIAAAGNHNVILQGP
                     PGSGKTMLAKGVKSLLPELNKKEILETTRIYSVSGLLNNTKGKIERPFRNPHHTATRV
                     SLIGGGRDLRVGEVTLAHNGILFLDELLEFDRRTLECLREPMENGEITISRNVGSVTY
                     PAEVMLIGAFNPCPCGNYLSGYEGRICTCSEYERIKYRNRLSKAMLDRIDIFTSVRYA
                     SYNELNSSNKKENLEDIRNKIKEARERQKFRFKDLEITYNSQMKHSHIQSLIRLDEEC
                     HEVLNNIYTRFSLSSRALDRIIKLSRTIADMNGSEDIKKSNIYEALNYRKNLEGEVI"
     gene            89030..89398
                     /locus_tag="K8M77_000081"
     CDS             89030..89398
                     /locus_tag="K8M77_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBI7084613.1"
                     /translation="MKKYNKSIGFYGENLSAKFLEKDGYSILEKNFNCSSGEIDIIAI
                     KDEIISFIEVKSRFSDSFGKPKESVTCSKQGRIINAAKYYLHIKKLYNYYIRFDVIEI
                     NFHIDSSKYELSFLKDAFRV"
     gene            complement(89399..90217)
                     /locus_tag="K8M77_000082"
     CDS             complement(89399..90217)
                     /locus_tag="K8M77_000082"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBI7084614.1"
                     /translation="MDNLIKDMRENLNSYSFKVVSDLVKELDVNRDNKAQIKELADLL
                     KEDKRKNVSSLGNRLEKNLNNLIKEEERVKNMYFFDKSFGDYKYVAGVDEVGRGPLAG
                     PIVSAAVILDSSNLDDIILYINDSKKLSEHKREELSEIIKEKALSYSISMCDSKEIDE
                     KGIGYCNNHVFIKACEGLNIKPDLVLSDGYLIKNFNGENKHVIKGDTKSACIACASII
                     AKVYRDNIMKEYHKKYPQYDFEKNVGYGTKTHVDALKEVGPTEIHRMSFLKNIL"
     gene            complement(90233..91078)
                     /gene="ylqF"
                     /locus_tag="K8M77_000083"
     CDS             complement(90233..91078)
                     /gene="ylqF"
                     /locus_tag="K8M77_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBI7084615.1"
                     /translation="MAINWFPGHMVKTKREIQNNLKLVDAVIEIRDARIPKSSKNPDI
                     DTLCAGKPRVILLNKSDLTDPKVTKAWKDSLTNDETIVLEVNALKGEGLNAIKPALLK
                     LLKEKHDRLKAKGLAKITTRAMVVGIPNVGKSTFINKMAKNNIAKTGDRPGVTKNKQW
                     IKTKLGIELMDTPGVLWPKFEDEIVGLNLAFTGAIKDEIMDTEELALKLVERLQETNP
                     EELMTRYKLTELHENPLDNLDAIARKRGAIMAGNQIDYNRIAGIILDEFRGGKIGKIS
                     LEKPE"
     gene            complement(91225..91572)
                     /gene="rplS"
                     /locus_tag="K8M77_000084"
     CDS             complement(91225..91572)
                     /gene="rplS"
                     /locus_tag="K8M77_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBI7084616.1"
                     /translation="MNEIIRAIEKEQIREDLTQFNIGDTIKVHVRIKEGNRERIQVFE
                     GTVIKKQNGGLRETFTVRRVAYGVGVERTFPINAPIIDKIDVVRRGKVRRAKLFYLRD
                     RVGKAAKVKELTR"
     gene            complement(91693..92403)
                     /gene="trmD"
                     /locus_tag="K8M77_000085"
     CDS             complement(91693..92403)
                     /gene="trmD"
                     /locus_tag="K8M77_000085"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBI7084617.1"
                     /translation="MKINILTLFPEMFDIFKHSIIGRARENGFLHIETVNIRDYTLNK
                     HKKVDDYPYGGGAGMVMTPQPIVDAIKAVKEKNKGKVIFLGPRGKTFNQEMAKELSKE
                     EELIFVCGHYEGIDQRIYKYFDLEISLGDFVLTGGEMACIPVIDSISRLVPGVLGSEE
                     SFQDESYYDGTLEYPQYTRPFEFEGEKVPEVLISGHHENIRKWRRKESLLITKERRPD
                     MFEKIKLSKEDIKLLKSK"
     gene            complement(92393..92890)
                     /gene="rimM"
                     /locus_tag="K8M77_000086"
     CDS             complement(92393..92890)
                     /gene="rimM"
                     /locus_tag="K8M77_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBI7084618.1"
                     /translation="MEDLLVVGQIINTHGLRGEMKVMPLTEDMRRFDYLEYVILKGQK
                     IKVEGVKYFKDKVILKLEGINSIEEAEKLKRIYLEIEREDAIELEEDEYFIVDLVGCT
                     VVDTEGFEYGKIKDVIQTPSNDVYWVQGKKEVLVPVLKDIVLDINMDEKLITIRPSGG
                     WQYED"
     gene            complement(92946..93173)
                     /locus_tag="K8M77_000087"
     CDS             complement(92946..93173)
                     /locus_tag="K8M77_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBI7084619.1"
                     /translation="MKELVEIIAKSLVDKPEDVHVNEILGDESIILELKVSPEDMGKV
                     IGKQGRIAKAIRTVVKAAAIKENKKVVVEII"
     gene            complement(93217..93462)
                     /gene="rpsP"
                     /locus_tag="K8M77_000088"
     CDS             complement(93217..93462)
                     /gene="rpsP"
                     /locus_tag="K8M77_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBI7084620.1"
                     /translation="MAVKIRLRRMGAHKAPFYRVVVADSRSPRDGRFIEEIGYYNPIA
                     KPEAEVKIDAEKAAKWLGNGAQPTDIVKKLFNQAGIK"
     gene            complement(93496..94854)
                     /gene="ffh"
                     /locus_tag="K8M77_000089"
     CDS             complement(93496..94854)
                     /gene="ffh"
                     /locus_tag="K8M77_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBI7084621.1"
                     /translation="MAFDGLASKLQDTLKKLKGKGKLTEKDIKEAMREVKLALLEADV
                     NFKVVKNFISNVKDKCVGEEVLNSLTPGQQVIKIVNDELTTLMGETESKLKYSDNGPT
                     VFMLVGLQGAGKTTMAGKLALHLRKKNKKPLLVACDIYRPAAIKQLQVVGKQIDIPVF
                     SMGDKVKAVDIAKAAIEHAKDNGNNVVIIDTAGRLHIDEDLMQELKDVKEVSNPSEIL
                     LVVDAMTGQDAVNVAETFNNSLDLSGIILTKLDGDTRGGAALSIRDITGKSIKFVGVG
                     EKMSDIEVFHPDRMASRILGMGDVLSLIEKAQQAIDQDEASKLSEKMLNQEFNFDDYL
                     SAMDQMKKLGPINKLIEMIPGVNTKELEGIDFSQGEKQMATVKAIIQSMTAKERKQPS
                     LVIGNGSRKRRIAKGSGTTVQEVNKVLKGYEMMKKQMKQMKSFQKNVSKKGFLSKLPF
                     MK"
     gene            complement(94866..95204)
                     /locus_tag="K8M77_000090"
     CDS             complement(94866..95204)
                     /locus_tag="K8M77_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBI7084622.1"
                     /translation="MENRFEISMLIDYYGTLLTEKQFNVMTLYYNEDLSLAEIAEINK
                     TSRQAIYDLIKRCSKQLHSYDEKLKLSKKVDKRYRIKEELMAELNKNSNLDEDIKKYI
                     DEKLEEIINA"
     gene            complement(95380..96294)
                     /gene="ftsY"
                     /locus_tag="K8M77_000091"
     CDS             complement(95380..96294)
                     /gene="ftsY"
                     /locus_tag="K8M77_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBI7084623.1"
                     /translation="MFGKLFDKLKTGLTKTRDNLTDKINEALNLAVTIDDDMYEELEE
                     ALIMSDIGMDTTIEIIDRLKAKIRKEKINDVEMVKPALKEVIAEMMLEGDSEEEEEDN
                     EKKVMLIIGVNGVGKTTSIGKIAARNKNNGKKVLLAAADTFRAAAIDQLDIWSQRANV
                     DIVKHQEGSDPAAVVFDAVQAAKARDVDLLICDTAGRLHNKKNLMDELAKINRIIDRE
                     LGDRKKETLLVLDGTTGQNAVIQAKQFMEACPIDGIILTKLDGTAKGGVVISIKNTLN
                     IPVKYIGVGEGVEDLQKFNAKEFAEALL"
     gene            complement(96308..>98682)
                     /locus_tag="K8M77_000092"
     CDS             complement(96308..>98682)
                     /locus_tag="K8M77_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBI7084624.1"
                     /translation="SLSKNKMTMLESELSNINEKLDSSKSKQVNYNNSLKINLATNLN
                     LQKELQIIEEEKKDKERELREKEEEFREGLKIIKETQGKLNQILSKVNKIEANKEMLK
                     KLENQYEGYNLSVKKIMASVDAGRVKINKNKCKVLGEVVETKAGYETSIEVALGGSIS
                     NIITEDEEVAKVLINHLRENKLGRATFLPLSIIRGKKLELSKNIVESEGFLGIASDLV
                     NFKEVYNNVINYVLGKTIICDNIDNAIKIAKMLNYRHKIVTLSGEVVNAGGALTGGSL
                     YQKNTGIMSRKNEVKALEDELVRVKNEEHILTKELEKLNSDQEELSIKKQEIIDKIYE
                     KNLELADLNNKIKNVNDETFKIKNSIKSLDLELKDIDSRKIRIVKEIEIVNEKISQYE
                     NKEVTNSEKVKELDKKREELSKTLSKYKDKLTNMKINKAKLQEVIEGREKEANRINSS
                     IEEVENKLINITKYLEDIKTNKEESKKEIVLCEKFIKEEEVNIITLEENFKSFELKKG
                     KLKENISSAENILEVLSVEIAKKEEEVHKNDITYTKQENEKNILNKRLLEEFNTSFEE
                     EKNRFKPIEDMDSHKVRISTLKARITGLGNVNVNAIEEFKEISEKYNFMTTERDDLEK
                     AKEELLNVIEEMTSKMRAVFRQNFNILNKLFDETFKELFKGGSAKLVLGEGDELTGNI
                     DINVQPPGKKLQNINLMSGGEKVLSAIALLFSILKMKPTPFCILDEIEAALDDANVRR
                     YAEFLGKFRDNTQFIVITHRKGTMEAADVMYGVTMEEKGISKIVSVDLDK"
BASE COUNT        30225 a        18459 c        10568 g        39430 t
ORIGIN      
        1 tattaactat atacctaata ttccaataaa agtttaacta ctatgatcag tttattatta
       61 gttatatact aaagcttagc gtttcttaaa tcacctaaat ttattatctt atagtttatt
      121 attaacctaa ataatctact ttgaataaat ccattaactc ttcttcactt aaagtattaa
      181 atgtacttcc tactcctaat tctccctctt caataatctt agaaatcagt tccttcttct
      241 cctcttgaag agctactacc ttttcctcaa tagttccttt agctattaat tttataacct
      301 ctactacatt tttttgtccc attctatgtg ctctatccgt tgcttgattt tctactgctg
      361 gattccacca aggatcaaaa tgaattacaa tatctgctga agttaagtta agtcctgttc
      421 ccccagcctt taagcttatt aaaaatactg agttttcacc ttcattaaat tcattcacca
      481 tatttattct tctcttagat gaaacacttc catctaagta actaaaagaa attttttctt
      541 ccttaagtct actacttatg ttttttaaca ctgaagtaaa ctgtgaaaac acaaggattt
      601 tatgcccttc ttctattcct tgatttagta tttcaactaa agcctctatt ttagcacttt
      661 cccccatata atcatttatt gttacagcag ggtctaaggc taattgtcta agtttagtta
      721 tataagataa tatttctatt ttactcttat taaactcctc tgcatcctgt ttcttttcca
      781 taattgataa agcattatta gcatatattg aatatacctt tttctgctct tctcctaaat
      841 caactaagtg catcttttca atcttatcag gtaattcttt tataacatct tttttctttc
      901 ttcttaatat gaaaggtttt ataaggctat gtatttcatt taatacttct tcactttcat
      961 ttaatttttt ataatatcta acagtgaatc tttttctatc ataaagatat cctggcatta
     1021 caaaatcaaa aattgaccaa agttccataa gagaattttc cataggggta ccagttaaag
     1081 caaatttata ttctgaatta acctctttaa cagcttcaga attttgagaa tctgggttct
     1141 taatagcttg cgcttcatca ataaagcaat aatcaaagtt tatcttctta tattcctcta
     1201 aatctctttt taataagtta taggtagtta taattacatc cttactctct atatctctta
     1261 aagcttcttc cctcttatcc ttgccttcat ttaatacctc cacaactaaa ttaggtgcaa
     1321 atttattaaa ttcttctttc cagttatata ttaaggatgt tggtgctact actagtgttt
     1381 tctttccctt attagaaaga ataaaggtaa ttgcttgaag ggtttttcca agtcccattt
     1441 catctccaag aattcctcct aagcctaaat gctctaaagt tttaagccaa ttgtatccaa
     1501 acttttgata ttctctaagc tcagccttaa gatccttagg aacttcaaag gacagtttat
     1561 ttatattttt aaatttactt ttcaattcat ttaacttctt tttcccctta atatatctaa
     1621 tccccttctc ttctatgtaa ttagctatat ataatgccct actattattt atttctaggc
     1681 aatttttctc taaatctcca ttatccactg aatctaataa ctttaaaaat tgctttaatt
     1741 ctagttcttc taaatcaaga aattctccac ttttaagttt aaaaaacttt aaattttccc
     1801 taaaagctct aagtatttct cttgtttcct cactaggtat atctcctatt ttaaagtcaa
     1861 actcaaaata attatatctt cctggtttaa tgtctccctt tattccttta cttcctaaac
     1921 tttttatacc tttaaagttt tctgaataat acacttctcc atactcttgt aatttgcata
     1981 catcatactt aaaaaagcta aatatatatt catctcctaa atagaaataa aactttttat
     2041 ttatctcttc aaggctaaat ctctcaatta atgcgattac ttctttttct ttttttatat
     2101 ctctatatat tattttttcc ttacaatcat caaatatatt aaattcataa gaaccatatt
     2161 ttacttttaa ggttaaagtt gcttctttcc cctctttatc aaagtaaaaa ttaaatttta
     2221 caggctctaa aaccgtctta ttcattatat ttttagaaag aactacattc tcagttaaat
     2281 cttttaagga aggtattaat tttctaaata ctctttcctc ttcacattta tcaatcttta
     2341 taacctttcc ttcagaaaat atatctaagt aattctttat tttataacaa aaatcttttt
     2401 taggaatata tatattagtt ccatataaga atacatcagc tctatctcct aaaaccttag
     2461 gcattccgtt taaggagctc agttcaaaat tattttttga atcctttaga gaaaaatcta
     2521 ttggaacatc ttcaaaaatc acttcacttt ctactgctct ataaaaaaat ccttcattta
     2581 aataaattct atgatttttt ataacacgaa aaaaatctct tactaaataa ttaggtatct
     2641 ttatgctttt accatctata aatgtatctc tatttcttaa gaagcttttt attggctttt
     2701 ctatctcttt taagctttct ataaactcta ttaactccct atccttagta cttaactttt
     2761 gttcccttat atcaaaagta aaagccttac tatatttaat aggtattcta ttatagtaag
     2821 ctactaaaaa ttgttcaata tcctttaata tataaagttt atttgaactc atagacttag
     2881 ttcctatttt aaactctgct aatatgctat tactccactc atttttattt atataaacct
     2941 ctatttttaa ctcttcttta tctgtttcct cttgaagaat taaatctaaa attttatttt
     3001 ctttcttctt agaaaataag tttttatcct cttcaaactc ttttttctta gctaaatccc
     3061 ctaaagcctt ataaaaagtt gctactaaat gcttacaaca ataattttcc ttactaaact
     3121 catgtttttc ataatcagga catgtacaat atgttgataa aatcccccta cttctaatat
     3181 ctatctctaa tttcgtacta tatttactaa ataattgttc tgaaattact attccattaa
     3241 cagctactaa gtcttcttcg tcttctattt ctactgactc cacaagatcg ttatttatta
     3301 ttctttgccc cttagcataa tttcttcctg tagtttcttc tttaaacctt tgtaacaata
     3361 aatcttcaac taacatattt actcctctat ttaagtgctt catatcttat aattataaca
     3421 taaatttatt ttctctgaaa aactatgtta actttacttt ttacaaaact tatttttaag
     3481 taaaatgaag aaaataaaaa tgaggctgta tcaaaatagt ttttaataag acttttgaca
     3541 caacctcatt taattatttt actgttcccc tctctactaa agaaggctct aaatttatta
     3601 ttttgctttt taaagattta ttgtttatta aatccataag tagtttacaa gaagattccc
     3661 caattttata tattggttga gctatggtag ttaattctgg ctccatataa gaacaaatgt
     3721 ttatattatc ataacctaat atgcttatat cttcaggtat tttatatccg tttcttatta
     3781 agtatttcat acctccaatt gccataacat cactactata aaaaattgca tctacctttt
     3841 tattattttt aattattctc tccgtggcat ttattccacc ttccatagag aaatctgatt
     3901 cttctattat ggtctcatta gatattccca agttctttgc agtatttttg aaaccttcta
     3961 gtcttaaatt agctatttct aaattagcct ttcctgtaac aaaggcaata tgcttattcc
     4021 ccctattaac taagtaattt actcctaatt caacaccttt cttgttatca caaaatacac
     4081 cattagcact ttttttatct gaaaccattc tatctatcat aacaaaggga atcttattat
     4141 tttttagaat tttaatccac tctttaccat caccacaagc tgctattata acaccatcta
     4201 ctaatttact aattaaaagt tttaagtatt cctcttcttt ctttttttca ttgagagtgt
     4261 tgcacaaaat aatactataa ccatgctttt cactttcaat ttctatggcc tttgccattt
     4321 ctgagaaaaa ggggttttga atatctggta ttatcatacc taaagtataa ctctttttag
     4381 tacttaggct tctagctaag gaatttggta tataattcat atcttttgca gctttaaata
     4441 ttttttctct agtctctttt gaaacattta tttctttatt atttaatacc atagatacag
     4501 tggtctttga tacatttact gctttagcta tgtctagtaa tgtagttttt cccattttac
     4561 gcaacacctc ttgtccgctt ccttttataa caacagtaat tataccatat tatgaccatc
     4621 tatacctcct actacttagc atatacaagc attatttaat attattaatt aaaacaaatc
     4681 taaaatttta ctacttattt agtatatacc ttaaggtctg ctgctatttt atttttaatg
     4741 ctttcaccct tatatatctt attagctaat tctactccca atgctccaat taaatctggt
     4801 tgttgagcta ttgtagctgt catttcatta gcatctatgg aatctttagc atcatcattt
     4861 ccatcgaacc ctataactaa tgcatttatt ccactagcct taattgcttt aactgcccct
     4921 aaagccattt catcattatg agcaaataca acttgtatat taggattagc ttggattata
     4981 ttttccataa cttgaagacc tttttgtcta tcaaaatcag cagcttgtat tgaaataaaa
     5041 ttaaatttat catttttatc cattatatta tggaatcctt ctcccctatc tctagtagct
     5101 gatgcacctg gaataccttg tatctcaact acatttattg gtcctttttc atctttgaat
     5161 ttatctaata catactcagc agccatttct ccacctttta tattatctga agctatatga
     5221 cttgttattt ttccaccatt agcttgtcta tctaatgtta ttactggaat attactctta
     5281 ttagctacct caacagtctt aactacggca tcactatctg ttggatttat aagtaaggca
     5341 ataactccta attgtattaa atcctctaca tttgctcttt ccttagctgg atcattttga
     5401 gaatctaata ctactaaatc atatcctaat ttttcagcct ctttttctgc tccatctttc
     5461 atatttacaa agaatgggtt attaagagtt gataatacca ttcctatttt aggcttatta
     5521 tctcttccac accctataaa tgaagtcata agcatcatga gcattaatcc aactgaaaat
     5581 atctttttat ttatattcat aaaactcacc tcttaagctt cttaattttt tctgctcttt
     5641 ttatctaaaa gtactgctat taatataact aaacctttta ctattgattg atagaatggt
     5701 gatacattca taaggtttaa tccattatta agtacaccta taataagagc acctattaaa
     5761 gtacctgtaa ttgttccttc tccccctgct aaagaagttc ctcctattac tacagcagcg
     5821 atagcatcta attcaaatcc tgttcctgca ttaggtgaag cagatcctat tctacttgtt
     5881 attattaccc ctgcaatagc tgatgcaaaa cctgatacta cataaactaa agtttttatc
     5941 ttatctgtat ttattcctga taatcttgaa gcatcttcat ttccacctaa agcatataaa
     6001 tatcttccaa atcttgtttg tgataaagca tatactgcta tgattgctat tattattgtt
     6061 attataactg gtattggcat aaatccaagt ttaccatttc ctattttaac aaaaacttca
     6121 ggtaattttg atattggagt accatctgta aatactaaag tagctcctct aaatattgtc
     6181 attgtagcta aagtaactat gaatgcttgt aattttcctt ttgatactaa taaaccattt
     6241 attaatccaa ctgctatacc aataacagct gccactataa ttgctaagaa tacgtttcca
     6301 gttgacttaa caatagaagc ccctatggca ccactaatag ctaaagtaga tccaactgat
     6361 aagtctattc caccagttaa aattacgaaa gtcataccta ttgctatgat tgcattaact
     6421 gaaacctgtg taaatacgtt agttatatta gatacactta aaaagtttgg agtaactata
     6481 gtaataacta tacataataa tactagtcct ataagagatt tatatttaga tatatttgct
     6541 ttaacattcg ctttcattat gtaattcttc ctaattgttt attccaacgg ctaatttcat
     6601 tatactttct tgatttgctt catctctatt taattcccca gagattcttc cttcactcat
     6661 aaccataatt ctatcactaa ttcctaaaac ctctggtaag tctgaagaaa tcattattat
     6721 gacttttcca ctagctttaa gttcatttaa aagttcataa atttcttttt ttgcacctac
     6781 gtctattcct ctagttggct cgtcaattat taatacttct ggagatagca taagccattt
     6841 agcaagtata accttctgtt gattacctcc acttaaattc ttaatgaact gctccttatt
     6901 tggagttttt atgtttatct tcttaatata gtactctata tcctttgctt cttctttttt
     6961 atcaagagat ttaaacttat tctcatattt ctttaagtta cataaggtca tattttctcc
     7021 aacagacatt cctaaaatac agccttcttt ttttctatcc tccgaaagat aacatatacc
     7081 attatttatg gcgtcactta tgcaatttat attaataaga cttccattta aagaaatttc
     7141 accacttgtc tttttatatt ctccaaatat tgtttttgca agctctgttc tgccagatcc
     7201 cataagacct gcaattccta atatttctcc ctttctaaga gtaaatgaag ctcctttaac
     7261 tccttcctta cagctaagat ttttaacttc taaagctaaa tctccttttt taacatctct
     7321 gtatggaaat tgatcctcta tggttcttcc aaccatcatt gctataagct tatcattatc
     7381 tatgtctttt atttctgcac ttcctgcata tttaccatct cttaatactt ctactctatg
     7441 acatatttta aatatttctt ccattctatg tgatatgtat attattgcta ttcccttttt
     7501 tcttaaattc tctattactt taaaaaggtt ttcagtctct acatcagtta aggctgtagt
     7561 aggctcgtct ataataatta ttctagcatt cttagttaag gcttttgcta tttctatcat
     7621 ttgcttttca cctacattaa gagtactaac taatctattt ggatctatat cacaaccaat
     7681 ttgttctaag aacattttac ttctttcatc taataacttt ttatttattc ttcctgtaaa
     7741 tttactatgt ttttcattac ctaaaaatat attttctgaa acagttaagt tatttaaaac
     7801 actaagttct tggtgaatta ttgttatacc agcttcttct gctgatttaa ttccctttac
     7861 ttctacttct cttccttcaa tgaatatttt accttcatct tttttatata ctccacttag
     7921 tattttcatt aaagttgatt ttcctgctcc attttctccc attaaagcag taacttctcc
     7981 accataagcc attaagctaa cattatctaa agctttgact cctggaaagg attttgatac
     8041 acctaccatt tttagcattg gtgttctttc acccatttac tacacctccc aaaataattc
     8101 ctattttgca aagaatattc cacccaataa atactgagca gaatattcat tttgaacttt
     8161 ttttaaaaaa caacccctga ttttaagatt atatttgcat atggagtttg ttctccagtt
     8221 ctaacaactg ccttacaatc ttttaattct ttctttaatt ccttatgtga tataaaagtt
     8281 attttagtat cttttataat ctcttttatt tctgtaaata attctggact taccttttct
     8341 gtttccttag caataagtac ctcttctact tgtaattctt ctaaaatagc ttttaaagtt
     8401 tctataaagc ctggtacgcc ttttattaaa gctaagtcta ttctctcagt ttcctttggt
     8461 ataggtaatc cacaatcccc tatagccaac atatcagtat gtcccatctt tgatatgact
     8521 gaacttatgt tactatttaa taaacttgtt tttctcatat tactcttctc ccttataaac
     8581 ttctaaaact tcctttagat atggtattga tggttgagcc cctttccttt gtactgctat
     8641 tgatgatacc ttatttccaa atcttatgga gctacttaaa gtctctcttc ctaaattttt
     8701 agtatctaac tttgaactta agcctccaat gaagctatct ccagcagctg tagtatctat
     8761 tgcattaact ctatacgctg gaactatttc acaaaaatct tttcctatta aagctgctcc
     8821 cttttcacca agagtaataa ttgcaaactt aactccttta cctaagaata tatccccagc
     8881 ttttttagca tcttctatat cttttatttc aattccagtt aaaagttctg cttctgtttc
     8941 atttggaact attatatctg tatattttaa taattgatct tttatttttt tagctggagc
     9001 tggatttaat attgtaactt ttccattttc cttagctatt ttaaatgctt ctattgtaat
     9061 atcctctggt gtttcaaatt gtgaaattat aatatctgat tcttttatta aattttcagc
     9121 actatgtatt tcctcttttg ttaatgtcat gtttgaacca gcattaacaa ttattgaatt
     9181 atttccatta tcatttacca ttattaaagc cattccagtt ggctctattc tatcttcaaa
     9241 aacatattta acatctatat tatcttctac taatttatct cttaactctc ttccattttc
     9301 atcttttcct attttagaaa tcatgaaaac ttctgcgcca cttcttttag ctgctactgc
     9361 ttgattagct ccttttccac cagctatttt ttgaaatgac ttagagagta tagtttctcc
     9421 tactttaggc atatctttaa cctttaaaac taaatccata ttcatacttc caagtacaca
     9481 tatcttattc attaatatcc ctccctagtt aaccgttcca ctgaaccgat taactatata
     9541 ataatacttt tgtaaacgat aatcaagact tttttataaa aatataaaaa aagagtatag
     9601 attttaaagt ctatactctt aaactagcta tttatttttc aactaattca tacatttctt
     9661 tagctctatc gacgtacata ataccttcta agtgatctat ctcatgcaag aaacatcttg
     9721 ctaaaagatc ttgagcttca taaactttta attcgccctt ttcatttaaa gcttgtattc
     9781 ttactgctct tggtctttca actaatccct catgcattac atagcttaaa caaccttcat
     9841 aatcttcata agtttccttt gaaactccta ttacctctgg atttataaga acatattcat
     9901 tctcaccatc tccaaaattt atataaacaa ctcttttatt aactgctatt tgaggtgcag
     9961 ctaatcctat tccttctaca gttgctaatg tatcttttaa atcttgtatt aatcctttaa
    10021 cttcattaac atctttaaca ggttctgaaa ccttttttaa agcttcgtgt cctatttgaa
    10081 ctattttctt aacagccatt taacttctcc tttcaaaatt cctataaata tattaaaaca
    10141 taaaaatata aatttttcac taataaatta tatattatat tctctataaa atattattga
    10201 ctgtctttaa agcatctaag ttaaaactta ttatacttta aagacagtca atacttataa
    10261 tattatcata attattaata atgatactaa gcttaaacat ccacttagta tgcatatata
    10321 tgacacaatt tgctttacag acattcctct ttcttgtagc ctataatgta tttggcttct
    10381 atcagcttca tagacaggct ttccagattg aaatcttcta aaaattacaa agatattatc
    10441 aaaaatagga actcctaagg ctaatactgg cactaatata gataatgctg ttgcttgctt
    10501 aaaagcacct tctaaggcta taatagctaa aataaaccct aaaaagttag cacctgaatc
    10561 acccataaat accctcgctg gatgtctatt atatcttaaa aaggctaata ttgaccctac
    10621 tagagttatt gacatatatg cagagttaaa ttgtcctttt gcaagagcaa caacaaacat
    10681 agtcatagca gatattatgg aaatgcttcc tgctaatcca tccattccat cagaccagtt
    10741 tattactgta gtaaccccaa aaatccatgt tattgtaagt atatattgaa tccatcctga
    10801 aaattgagta aactctccag taaatggatt agttatacct ctaaaaacaa tcccagcttt
    10861 aaaaaccata gttgcagcta aaatttgaat tataaccctt ggataaattg gaaactcttt
    10921 gccctttccc ttataatagt catcaatcac tcctattatt attaccaaag tagatgctat
    10981 aaatataata aaagtttgtg ataattgcct tgtagaatct attccaaaaa ggtatgttgc
    11041 aaaaaatcct atatacataa caaccccacc tattaaaggt gttaatttat catgtttttt
    11101 tcttcctgta ggcttatcaa caaaaccctt tttagttgca aattcaataa actttggcat
    11161 taatagatat actatgacaa aaggaataag cattacaatt aaatacttaa tcattttttc
    11221 tccctctgta ttaaaaacat tttttcttac atatactatt ctagtagatt aataagtcat
    11281 attaatgaca ttttaattaa aattatttta tcacttctta ttatatcact aattaagaaa
    11341 tttaatcata ttttaataat tactctactt catgttttaa gcaaactgaa aatacatgac
    11401 aaggcatttt tccttcttct tttctttcgt attttttaac cagttctctc atttgtctat
    11461 ttacttcttt aaactcttct tcatttaagt atatgttctt ataattgaat cctatggaat
    11521 cctctgcatt accagtatct actttctcag atattctttt catagtttgt tcctttgaac
    11581 tatcgtaata ttcacttaga atatttaata tttgagattt atagacttgt ttagcttctt
    11641 ttttaatttg atttccctct atattgaagc tttctgcagt tagataataa tattttgcta
    11701 ttattccctt tatttctttt gtttttacaa gctctaaaat gcctgctttt tctaattttt
    11761 taatatggta gtgaactttt gctggtactt ccccaaaaaa atcagcaatt tcctttaccg
    11821 tagcagattc ttgattattt ctaagataag ttattatctt caacctataa ggatctgaat
    11881 atgcttttat ttgttctaaa gttgttaaat tcataacact gttcatttta agcccccttg
    11941 atatttcagt ataattttta cttattatac catattttag atattttata tataaaaata
    12001 caattaaaat atcttttttt cttaataaaa ttatacattt tatttttaaa atagtaaaga
    12061 aaaaaggcaa atcttgatgt tttgtcaaca taagattgcc ttttttatta atttaaataa
    12121 actatcctct ttgctctatc atagacttaa ctttcataag tcttgttaag gcacttctct
    12181 cattttcatc aagcttcatt ctgatgtatc tgatagtttc ttttaattga ggaattgtca
    12241 tatattcaag agcattaact cttcttcttg tcttttctat ctcatctgcc ataagctgac
    12301 cagatttttc aacttctgca agttctaata actttggtaa aattccatat agtttggaaa
    12361 ttgcatcatc aagttcagct gaagtattca taaagccata aggataaata ctaccttcat
    12421 caccttctaa ttttcttttg aagttcatta taggcacatt tacactcatt atgtttttat
    12481 ttcccacttc aacctcaatt tcttctttag ggtatgctat agcttcctct aggaactctg
    12541 aactcataac agctctagcc ataacgaagt cttttaagga acttcctagt tccttttcaa
    12601 cttcatctct taattgatta ttatatttga taagatttat gaattgtctc attaactcat
    12661 cttgcttatc ttttaataat ttatgtcctc ttgttgctgt tgctaatctt ttctttagct
    12721 ttgagagttc cattctagta ggattgacat ttaatctttg tgccattctt actcatcctc
    12781 tccaacaggc atatactttt caagatattc atctctaatt ctcttaagct ctgttctagg
    12841 taagatctta agtaatttcc atccaagatt tagagtttcc tctattgttc tattagttgt
    12901 gaaaccttgt gaaacatatt cattttcgaa ggcttcagca aatttagcgt atgctttatc
    12961 tacatctgat aatgctgatt ctcctaagat agcagcaagt tcttttgctt gcttaccttg
    13021 agcatatgct gagaataatt ggttcattgt atcagcatga tcttctctag tttttccttt
    13081 accaataccc ttatctttaa gtcttgataa tgatggtaaa acatctattg gaggcattat
    13141 tcctttcttg taaagttctc ttgaaaggat tatttgtccc tcagtgatgt atccagttaa
    13201 gtctggaatt gggtgagttt tatcatcttc aggcattgtt aatattggaa tttgagttat
    13261 tgaaccttct tttccaacta atcttcctgc tctttcatat aatgttgaaa ggtcagtata
    13321 taagtatcct ggatatcctc ttcttcctgg aacctctttt ctagctgcag aaacttctct
    13381 taaagcttca cagtagtttg ttatatctgt cattataaca agaacgtgca ttcctttttc
    13441 atatgctaag tactcagcag tagttaatgc cattcttgga gtagctattc tctcgatagc
    13501 tgggtctgac gctaagttca taaataaaac tgatctatct atagcaccag tttttgtgaa
    13561 ctcatctacg aagaattcag cttcttcgaa agttatacct atagcagcga atacaactgc
    13621 gaacttagaa tctgagttta aaacttttgc ttgtcttgca atttgagcag ctaactcttt
    13681 gtgtggaaga cctgatcctg agaaaacagg aagtttttgt cctctaacta gagtgttaag
    13741 tccgtctatt gcagaaatac cagtttgaat aaattctgat ggatagtttc tagcaactgg
    13801 gtttatagct tctccattta tatctaatct cttctctggt attatatttg gaccattatc
    13861 ttttggtctt cccataccat cgaatactct tcctaacata tcttctgaaa caccaatttc
    13921 aagtggtctt cctaagaatc ttacttttgt atttttaagg ttgattcctg ctgaaccttc
    13981 gaaaagctga accatagctt tatctccgtt aacttcaaga actttacctc ttcttaactc
    14041 accagtttgc atttctattt caactagttc atcgtattta acaccttcca cgccttcaac
    14101 agccattaag ggtcctacaa cttctgtaac tgttctgtac tctttaagca tttagaacac
    14161 ctccctcgct gattaacttg ttcatctctg attttaattc aactaagatg tcatccatct
    14221 tacttatttc ttcttctgaa atatatttac ttcttgcaat tctatctcta acaggaagtt
    14281 ttaatacttt atctaaataa actccagctt ctaaagctct ttcagcctca tgtttgaaac
    14341 ttaagattaa gtttaacatt ttatactgtt tatttaatga agtatatgtg tcattctcat
    14401 ggaatgcgtt ttgttgtaaa tagtcctctc ttattgactt agcaacttca agttttaatc
    14461 tatcaccttc tgaaagtgca tccataccaa caagtcttac tatttcttct aagtttgctt
    14521 cttcttgaag aagtgccata gcttctgttc ttaatgctga ccaatcatct gctacgtgag
    14581 catccatcca ttcacccatt ttttctaagt ataatgagta tgatgttaac cagttaattg
    14641 atgggaagtg tctcttatat gctaattgag catctagtcc ccagaaaact ttaactattc
    14701 ttaaagttga ttgtgtaact ggttcagata tatctcctcc tggaggggat actgctccga
    14761 tagctgtaac agctccttct cttccatctt tacctaaagc tacaacctta ccagctcttt
    14821 catagtaatc agcaagtctt gatcctaagt atgctggata accttcgtct ccaggcattt
    14881 cttcaagtct tcctgacatt tctcttaaag cctctgccca acgtgaagtt gagtcagcca
    14941 tgattgatac tgagtatccc atatctctga aatactctgc tattgttata ccagtgtata
    15001 ttgatgcttc tctggcagca actggcatgt tagatgtatt agctataaga actgttctct
    15061 tcattaagct ttccccagtt ttagggtctt taagttctgg gaattcgtta agaacgtctg
    15121 tcatttcgtt accacgttct ccacatccaa cgtaaacaac tatttcagta tctccccatt
    15181 tagcaacttg gtgctgaact actgttttac cagctccgaa aggtcctgga acggcagcag
    15241 ctccaccttt agctactggg aagaaagtat ctataactct ttgtcctgta gtcattggct
    15301 caactggatt taattttctt gcatatggtc taccttttct tacaggccat ttttgcatta
    15361 atgttaaatt cttatcacct ttttcagttt ctactacagc cataacttct tctacattga
    15421 aatctccagc tttgatttct tttattgtac cttcaacgcc gaaaggaacc attattctat
    15481 gaagaacaac ggcagtctct tgaactgttc ctattacgtc tccagctgaa accttatctc
    15541 caactttagc agttggcaca aaaggccatt ttttctctct atttaaagat ttaacttcta
    15601 cccctttact taagaaagca gaattagctg ctttcatgaa agcgtctaga ggtctttgta
    15661 ttccatcgaa cattgactct ataagtcctg gtcctaattc tactgaaagt ggttctccag
    15721 tagttataac tgggtcccca ggtccaatac ctgatgtttc ttcatatacc tggattgaag
    15781 ccttatctcc tctcatttca atgatctctc cgataagacc tttttctcca acttttacaa
    15841 cgtcatatac attagcttca tccatacctt cagcaactac taaaggacct gaaaccttga
    15901 taattttccc cgttttcaag ttatctacct actttctata aaatgttaac gccaactgct
    15961 ttttcaacgt tatctgatat tctttgtttt cctatattta gagttccttg attgcttgga
    16021 attaatatta cagcaggaag tacttgtctt gtatatctct cgatagtttc ttctatattc
    16081 ttagctacgt gctctgtaac aaagattact gcataacctg tcattgcaag cttatcaatt
    16141 gtttttctag cttcttcatc ttcaactaca gggaacacat ctattccaag agctttgaaa
    16201 gctaaaactg aatctttatc acctacaact cctattttct tatacatatc catcacgcag
    16261 cctttctctt attacttcct tagtaatatt gtttaattta ccaaccatga tagttcttat
    16321 ttgctttatt tcattctctt taacatacat atatgttaaa actggctcta aacctttagt
    16381 tactatttta gcttccttca tcatactcat aatgtgatta tcaataagct tttcaaattc
    16441 actaactgat gaagtctcta tgtaactagc tataccttct cttagtactt gtgaatattc
    16501 tttactactt aagttgtttg gaatattttc gattgtatct aagtataaac ttctaaactt
    16561 acttgcatct atactaccac ctggtactat agcatctaaa aagaagttaa catttttatc
    16621 ttgcttttta gctcttaata atgtttttac attatttaaa tctataagat tagtaagata
    16681 tctatctaaa gccttatctt ttatattttt cttatttgca atttcatgaa tatgagcata
    16741 taagtattga tctgctatta aatctataaa ttgaggatca ttagtttcct caaatttttc
    16801 aaatattttt cttaagcact cttccatttt cttaggaagt ctaccaaaat cttctccatt
    16861 tattgcagat tttaaatcat ttactcctac cataccaact ggtattagca tttgggaaaa
    16921 atctttatct aagatttttc ccttaactaa cacttttatg ttatgatagt catattttaa
    16981 agccataaca tctatgattt cttttaaagg actagcttca tacatagttt tatataatct
    17041 aactaactca tttgataaaa gaatttcata atcatctggt cttcttactt cattcataaa
    17101 tgctgcatac tctgtttctt gtagaacttt taatgcacct tctgaagtgt ttgcatctat
    17161 cattctttct atttgagctt tatcaaggag tttcttttcg tatactctga ttctaggaat
    17221 taattgcaaa aattgcattg aatccattct tcatcctcct taatcaaata atactttgtt
    17281 cacctcaaac tctaactctc ctcttaaaga gttgattaaa gcttcaaagg tattatttat
    17341 ttgtattcca tttctatcta atatgaagcc accattaaat ttgccttcaa tatttaatgt
    17401 aatacttcct ttttttcctt tagattcaag ttctttattt aatgcttcca agaactcatt
    17461 gctaaatata tctttatctt tttcattgat tataataact tctgttcctt ctaactctaa
    17521 agaatctaaa gttgaaataa cataatattt ataatctcca ttagaaagat tttgtaattt
    17581 ttctatagct tcgttaaata cttttgatat aacgtcttgc ttagctctta acatatcatt
    17641 tcttactgat aatttagcat ttgaaattat tcttgccttt ttaacttctg cttctatatt
    17701 agctttagtt attatttctt ctttatccgc ttctgctcta ttagttttct tagatattat
    17761 tttatctttt tgggcttcag cttcagctaa tatatacttt ttcttttctt cagcatcatt
    17821 tagaatcttg gaagttaaat tatttaaatt tgacattccc ttcacttctc caatctttgc
    17881 ttactatttt attacgcttg caccatttac taataagaat gatataacga atcctaataa
    17941 tgcatatgtt tcaaccattg ctgcgaatat gataccttta gtattgtgct ctggtctttt
    18001 agctaaaatt tggataccag ctgcagctgc tttaccttga gatatacctg accataatcc
    18061 agcgatagct attggtaaac aagcaaataa taagtataat cctttagcta atgatgcatc
    18121 accattactg atttgattaa atactaagaa acctataacg aatccataaa gaccttgtgt
    18181 acctggtaat aattcaagaa ctaatgcttt accaaacttt tctggttctt cagtaactaa
    18241 tcccgctgct gcttccccaa ctataccaac acctttagct gatcctatac ctgacatacc
    18301 tacagctaag gctataccta aagctccaaa tattaatcct ccgtaatttg ccataaaata
    18361 tgaaatccag ttttccataa tttaattcct cctgaaaata taatgttatt ttaatttaat
    18421 tttaactata atttatttat ttctttttat tgttatgaat ttatttattg ttctaaatgg
    18481 tgtgaatgct tttccgccac cttcatagaa ttttgagaag tactcaacgt actgtaatct
    18541 cgctgtatga acatatgctc caagtaatga taaaagcatg ttaaatgtat gagctgctat
    18601 aaagaacaga ggtcctacaa ttataactgc tattcctggg aacataccta ttattagatt
    18661 aagtgcagct gctattgatc cacctgcaat acctaaagcc ataagtcttg tatatgatac
    18721 taagtctcct acgtatcctg ttataccgta taaagcatat aaaccttgac ctatttgagc
    18781 accttttgtt tcttcatctc taccttgtgt taagattatt aatattgaac ctactattga
    18841 taacacttta cctactaagc ttatagtacc atcaaagaat atcattggaa gtcctattaa
    18901 aagcataatc catgatccta catcaaataa agcatataaa tattgcttat ctctgattaa
    18961 catgtacgct tttataaata atccaaagaa gatttgaata accccaaatc caactgataa
    19021 aattagtaac aagttggtat ctttacttgg atttataagt ccaaatttta attcaaatcc
    19081 aaaggcagta ccaaatattg cacctgcaat agttgtagct atacttaaat acataaagaa
    19141 tttagcaaac ttttcttgtg cctcatctaa attaagtaat ttcatagcta atagtgagcc
    19201 tacaaataat actagtccgt atcctaaatc tgcaaccatc atgccgaaga atattaggta
    19261 gaaaggtgtt aaaagtggtg taggatctat ttcatcatat cttggcaagc tatacatttc
    19321 tgttattgaa tcgaaagctg ctgctggtcc tttattgtgt aatttaactg gaacttctgc
    19381 aacctcttct tccttaactt cttcaaactc aagataatac atatctccta ctgaactttg
    19441 aataattcct tctaaagaag aattatcatt tttaggaacc caaccttgaa taacaactac
    19501 tctatctgtt tttaagaagt tttcacttgc ttcttttcta agaagtatat tgctgtaata
    19561 ttcataagct aattctaaag ttctcttttc ttctctgtaa tttgaaagta ctccttttaa
    19621 gtctgatttt ttagcttcga tttcttgtat ctttaattta aactcttcaa caagaactga
    19681 agctttttct ttgtacttag tttggaaagc tgaaaaacca aagttcttta aaacttcgtc
    19741 catttcttca gctctttctt tcattgtcat tacaaagaca tatgaatctt ggtcagtgtt
    19801 agaaataact tctatataac tttccttgaa ttcgctttca atttcgttct tcatattttc
    19861 aagattttga cttgaaatca cacctaagaa cgcaactact ttttcaaaat tatttaattc
    19921 acctagtgga gcatcgaaac tttgccatgg ctctaagatc tctatttcac ctaaaagttt
    19981 tgtcttttca ttttctagag tagcaagctc tctctccatt ttcttaacct ctgaataaac
    20041 gacttcccag ttgctatttt caacctttac ttctagctca tccaaagtta aactttcttt
    20101 accagctttt aaagccttaa gtcctccgac ttcagcaaca aactctttta agaagtctaa
    20161 agctgatctt gtttttgaaa cattttcttc tataagagtt gtttcagcat ctaaatcatc
    20221 tttgttaaga gattcaaaga cttcattgcc ttccactttt tcttcatctt gtaagtttat
    20281 gaactcaact tctgaagaac tttgaatttt tttaagaagc ttttctttct ctgattcaaa
    20341 ggcaagtaag gtgaatttat tcatcttaac tattgccatt agttttcaca atcctctcaa
    20401 taactaaatt tactgctgaa tcaatcttag aacctttaag acttgatacc tcagcacatt
    20461 ttttttcacc ttctgaaagt atgacctcag cctcttttct gccggcttct tctgcattgc
    20521 taataattcc ccttgcaaca tttttagctt cttctattat tgaattatat ctctcatcag
    20581 cttctattgt tgcatcagaa attatttttt tgctttgctc atgagctttt ttaatcattt
    20641 catcagcttg caattcaact tttttaattt cttttattgc ttctattgcc aaagtttcac
    20701 cacctttata taaggaattt ttataaaata tatattaata ctttatatag tattaattaa
    20761 cccttaatat taattataca tgaaaattaa cttaattcaa tagaatttta ttaatgtata
    20821 cattttcaaa gatttttttg caaaaatcca cattacattt tttttaataa aatattaaaa
    20881 ctattaacat tcacaaaaag tcgtattaat atctttgaat atcgtatatt aatacgacta
    20941 atatactaat aaatttttaa ttttccattt atttcttttc tacttctaca tatgaatcgt
    21001 agtcaaaatt catttcatca aatataagat attttggttc aaagttaatt tctccatttt
    21061 taatgtaatt aattatatct atttcaaatt tttctatatg agctttttta aatggcattt
    21121 gtctatcaaa actatctttt atattttgct catcttcttt tgttatagtt ctaactaaaa
    21181 agtctggtaa ccatctcttt ttattaaagc ataaataatc aaattttaaa acttccttaa
    21241 atagaccttc atcctttcct cctatcttct caagataaaa atctaataat atcttataat
    21301 attcaacatt tcctatgctt ctcttgaaat tgcccttctc ttcaaagaat atagctaaat
    21361 cataataaaa gtcaaaaggt ttttcatata tatttaagaa gaatttaact acattgttaa
    21421 atttacatga attatagtat ttatcaacca ttgcttctac tttttttaat aataaaagtt
    21481 cttcataact tatatcttta cttcttatta tttcatatgg agcataaggt gaataaacta
    21541 ttccccactt tttggcttcc tctctcattg aagatccctt taataatttt aggaagccta
    21601 actgaatttc atctggtctt atattatgaa catcattaaa agatttttta aatgactcta
    21661 aatcttctcc tggtagtcct gcaattaaat ctaaatgttg cattacattt tttcctgctg
    21721 caacttttaa tactttctct tttatatttt catatgtaat atatctattt atattatgta
    21781 gaacttcatt gtttgtagtt tgaactccaa cctctaattg gaatcttccc tttggagcat
    21841 tatttaatat ttctatttgc tcctctgtta aaagatctgc tgcaacctca aaatggaatc
    21901 ttgtctcagt atcctgttcg cttaaatatt taagaagctc aactgtgtat tctctattac
    21961 agttaaaagt tctatcaaca aacttaacta attttaatcc tctttccatg aagtatttta
    22021 attctttctt tactctttct acatctaaga atctaactcc atgcattaca gatgaaaggc
    22081 aatatttgca cttaaaagga cacccccttg aagcttcata gtaaacaatc ttattgttta
    22141 tatcttcttc ataagtataa gggaatacta attcattcat atccatttca ggtcttttag
    22201 gattttcaaa gactttttcg tccctcttat aatttaatcc ttttatatct tctatttttc
    22261 cttctccaag cttataaaga acaaactctc taaaagtttt ctctccttct cctactatta
    22321 catattcacc ttcatgattt tctaaaaatt cttttccttc ataagaaact tctggtccgc
    22381 cataaagtat ttctatatta ggatcaacaa gttttataag ttcagctagt ctatttacaa
    22441 attccatatt ccatatgtaa caggagaaag ccactacatc tggtttctcc tcaataatac
    22501 cttctaaaat attctcaact ctatcattta ttgaaaattc ttttatatca ccttggaaat
    22561 ctaaatcctt tgtaaatgcc ttcaggtacc tcactgctag gttgctgtga atgtactttg
    22621 agtttattgc tgttagcagt attttcattc tttatacttc ctctctttat tcctttgata
    22681 aatattattt tatcacaaac tttagtttcc tctatttcaa aatatttttc aagtattttt
    22741 tttgagctat ctactgtaaa ctcattagaa atactattat ctataaattc aaactctttt
    22801 tctttatttc ctccaattaa aactttaacc ttatcattta cagtttcttt tttttgcttt
    22861 tctctatccc atatatataa gtttcctcca aatctcacat tgctccatat attttttaca
    22921 acatcttcaa cctcattttt atgcaattga cttaaagaaa agaaaagcac accattatca
    22981 tattgatttc tatattctcc attaaaattt tcaacataat aatctacact tatttcactt
    23041 tctgtttgct ttaaaatatt ataaataatt ccatagttat ttataccaac atctaaagta
    23101 ttacctttaa aatttatgtt actcatatca agtataactt ctctcaccat aatatcactc
    23161 ccaatttatt ccatattaat ttctattata catttaaaga gtaattcttt ctatgcttat
    23221 tagaaaaaat tactcttata attttatttt ctataattat ctttttatat tttaaatttt
    23281 aaactaatta cttaatttat tttttcttta aggatagtag gaacttaata cctaaaactc
    23341 caattaatat aaagtttatt cctaatataa caaagtaatt ataaataggt atgttattta
    23401 tagtgttact tttttctagt ctaaaaagta cccccttatt tccatctagt atataaagat
    23461 tcttccctat tattttcata tcatttatat atgaattttt atctaaatat attaactctt
    23521 taacttctcc ctcctttaat aattttaaaa gagtattgtt tttgttgtta aagaataaaa
    23581 atgaattctc atcttctaga attattcctt ttttatctat taataatgta tttatatttc
    23641 tagtgtcttc actttggtat agtggtttag gcattacata gcttttatgt gcatctgtta
    23701 caaagttaat tataggtttt ccttcttctc taaatcttgg agaattaaca ggatctcctc
    23761 cactataatc tggccaacca taccaagttc cctttccttc tattttatat atataatctg
    23821 aatctccact taaaggtcta accccctcat cttccattcc tccaacaact gcaaaaatct
    23881 ctcctgaact atttaaatca aaacctttaa catttcttat tccataggaa tataattgct
    23941 ttttcttgct ctctatatca aattctataa tagctccatt acctaatata tttcctttta
    24001 ccttttctcc ctttactgtc ttagtattat agggcgcaaa agctcccttt ttattttcat
    24061 cataatttct tccacttaaa acaatatcta ctggaggaat atcaggattt tctccctctt
    24121 tatctactat tccagaattt gtagaagttc ctatggttaa ataaagcttt ccatcattta
    24181 ttaaaatcct agtatcctct ttatttatcc ccttattagg aatatcctca attaaaactt
    24241 ctctttctga actttttatg ttataagata ctaacttttc atctaaagtg taatataaaa
    24301 atttattata atattctaaa tcatatatat ttccctcttc ttttaattcc aaaaacacat
    24361 tattatttct atctattttc aaaatcttat tttctaatgc tatataaata ttctcttcat
    24421 cattacataa ggctcttgct ccctttaatc cctttagtat taactcttca ctaaaacttt
    24481 cactttctaa atatttacca tctttattag taaaaaaaca tattatactt cctagtaatg
    24541 cactaattaa aattaaactt ataaacttta taaaattctt catatttcct ccctaaaaag
    24601 cctcaataat aaattttaac aattaaaaac tcatagataa ttaataataa ttacctccta
    24661 tataatgaat atttaacata agttgtgaat attcattatt attgaacttt taaatagggt
    24721 ggtgattcat tgagtaaaaa tgatttctat aaaaactctt tcatgctaac agcctctaac
    24781 ttaacaactg gattattagg ttttatcttt tctatgtatc tatcaaaggt actaggccct
    24841 gaaggaatgg ggctttatgg tattataatg cctatttata atttatttat atcaattatg
    24901 acagctggta taattgcatc catttctaag ataactgcta tatattcagc aagagatgat
    24961 tataaaaaca taattcgaac aatgaaagtt gtggctattt ttaattttat ttggtgtctt
    25021 ataataggaa tatttgtatt tttcttatca cctataatcg gacatttttg ggctaaggat
    25081 cctagaataa taaaatctat aatgataact tgtccagcta tgatatttat agcactttca
    25141 aacatattaa agggattttt ctatggaact tcaaaaatca ctgttccttc ttttatagat
    25201 attttagaaa aatctttacg tatctttgtt ttagctatat taatttttat atttaaagct
    25261 gaaactttag agtctttagt taccttagct tacttagcct tatgtcttgg ggaactacaa
    25321 agtttaatac ttttatttgg atattttaaa tactcaatga gtaaatttcc aaaaacaaat
    25381 gcaaaaggtg aaagccgtgc tcaattatta tttgatgttt tagtaacctc agtaccttta
    25441 tgcttaaatg gatttttaat gagtattttt agcgttatat caacactttt agttcctaaa
    25501 cggcttatag ttgcaggatt tacttattcc caagcacttt ctcttatagg aaaatattct
    25561 tccatggcaa tgtccatagt aacttttcct ataattatag tttcttcaat aaatactatg
    25621 ctgatacctg atttatctca aactttaagt aaagggaatt atctttcagc tactaaaaga
    25681 attagagatg ttattaaaat agctttttta ataggtattt gtaccacagt aattggacta
    25741 tgtgttcccg actctttagg taaattattc tttggaagag atgatttagg acaatatata
    25801 agaataacat cagtaatgat gccaatagta tttacttcaa atactatgta tggaatttta
    25861 aatggacttg gaagacaaaa tgtaatttta agaaatacta taataacaga agttttagaa
    25921 gttacattgt tatttttctt aactgcaata ccatctataa atatttatgg ttatgcaata
    25981 actatgctta taatttcatc actttcactt tgtttaaatc tttatgaaat atataaaaat
    26041 ataaatatag gtttatcctt atcaaacttc ttaatatata tattaacagg agttttaacc
    26101 tatatatgct taagtccact ttcactaaag ctttctttta ttgattttag agttcaagtt
    26161 ttagctgtaa cttctatagc agcttctata ttcatatttt taataataaa ggagaaattc
    26221 tcatcgaggt ttaaaaaaat ctctttaaaa agcagataat aggatatata aaaatataat
    26281 aataaaactt acaaacatta tgaagagaat atctagtaat tctcagaata tgtttgtaag
    26341 ttttataaaa agttattttg atgtgtagcc ttcctttttt gaaagaacct taaattcttt
    26401 attttctcca agaactctca ttatttttaa tcccttttct tcatttaagt tgtaatcttt
    26461 tactcctagc ttttctatgg atttcccatt atctaaatca tctattataa ctaaaggttt
    26521 taacctctta actaattttt tcacttcttt tttattttct ttctctgaat caattaaatt
    26581 taaaatctca actctacaat cttctatatt actaaggtta attgcactat cataagcctc
    26641 ttctgataaa acagctagct taatattatc tacagtagca aaaatcatac cttccttaga
    26701 attctctact ttctttgcat ttagagaaat gctttccttg ttataattaa atccctcttc
    26761 catagatgct atttctatat ttttttcctt tgtaagatta cttacctctt cacttactcc
    26821 atttaaagtg ggaagaagta ttttctttac actactttta tttatgaaag tttttataag
    26881 ttcatcagtt tcttcttcca tagatcttaa aaccaacaaa tcaatttcct ttaaattttc
    26941 tttatttatt ccttctatga ttctctctac attatcacct tcacttataa gaacagtttc
    27001 accattactt cttaaaatta agatttcttc atcatagctt agaaattcta tagtattagg
    27061 attttccttt gccaaagcag tattacctcc aagaaataaa cataatatta aacttataag
    27121 aaatattatt ccatttactt ttattttcct cataaaattc accacctata ccaataaaat
    27181 cccctaaaaa ttatttttta tactacttat agtaataaaa aagaactata tagagtaaaa
    27241 actcaatata gctctttttt ttattatttt aatctagtac caaattcatc ttgctcaatt
    27301 aaaccttttt tcataagtcc acctaaggcc attttaaagt aatttttaga agttttaaaa
    27361 gtatttctta tttcatctgg agtactctta tcatttaagc tcataactcc accattttct
    27421 tttaagtaat ttaatatagt atctcctaat tcatttcttt ccttaagttt atgatttctt
    27481 aaagataatc ctactattcc atcttcataa acctttatta tcttaccttt tatattatct
    27541 cccggtttaa tatttgtaaa atattcattt ttaagaacca cagctctatg tttattatct
    27601 attgctactc ttaaagaacc attagtttgg tatccataaa ctattcctga aacttcagtt
    27661 cccattttag gttcttctat ttcatctaag tatttatcaa cctctgttgt tgctgctaat
    27721 cttcctgttt tatctacata aatatatact aagtactttt taccctcttc cattttatac
    27781 ttttgctcct ttaatggaac aagtatatct ctttctaaac caaagtctac aaaagcacct
    27841 attttagtaa tagatttaac ttctaataaa gcaacatctc ccgcaacagc taaaggcttt
    27901 tttaatgtgg ctataagtct atctctagaa tctctatata taaaagcttc tacctcatct
    27961 ccaaccttta tttctcttcc atctaaattt ccagttggaa ttaatatatc atcagaatta
    28021 tttctagttc ctctgtctaa gtagtatcca aaatccgctt ctcttactac tttcatttta
    28081 ttaaattgtc ctatgtttat tatcataagc tcctccttta tttaatataa agatttttca
    28141 tctttacata ttctcttctt gaatttttta tactttcaat ttcatcctca gtaagttttc
    28201 taacaacctt tcctggaact cccatgacta atacgccctc tggtatttcc ttgttttgac
    28261 ttactaaagt tcctgcagca atcatagtgt tttttcctat ttttgctcca tttaatatta
    28321 ttgctcccat accaattaag cattcatcct ctatcttaca cccatgtatt atagcaccat
    28381 gtcctatagt aacaccatga ccaatttcaa ctttatattc tttatcacca tgcaatactg
    28441 cgttatcttg tacattggtt tcatttccta ttttaattaa gttatagtct cctcttatta
    28501 cagatccaaa ccaaactcca caatctcttc ctatagtaac atcaccaatg atatcactac
    28561 tgtaagctat aaatgttttt tcccctattt caggcttctt tcctctaatt tcaattaaca
    28621 tgtgatcacc tcttagtcat tcttgtaatt ttatcatatg aaagtagaat atgtctactt
    28681 atgcttttgt atgcttaatt taatcttttc tatttcaaag atttccctaa taaaaattaa
    28741 gatactttag tttaaatata cttaatatta tatacttaat attatatact taaaaaatca
    28801 tatataaaaa taataaataa aacacattta atctaaatat tataaatatt atatactata
    28861 ctaatattta tagagaggta ttaatggata aaaaatctat accctttgat aagcttaagt
    28921 tttattatgg aattccccat tcccatacat ccttatcaac aggaaaaagt agtccttatg
    28981 aatccttaga acatgctcgc tctaatggat tagatttttt aataattact gatcataata
    29041 aatatttaag cgaaactatc aaagaaaaaa atagagaaat ttcaaaatgg gagtatttaa
    29101 ataaatgtat aaataaattt aataaaaaac attctgattt tttagcctta tgtggttttg
    29161 aagctaaatc aacaacctta ggtcacttaa atatcctatg tcctaataca ttttttactg
    29221 gaataattcc agacattaaa tatctattac tttggctcat taatgatcat acagccttac
    29281 ttagtattaa tcatcctaaa aattccatag ctaaaaaaat tgaatttacg cctattttag
    29341 ataaactttt atgttctata gaggttggaa atggaattca tcctagtact tatacaagat
    29401 atgactctca atttttctcc cttttagata agggatttaa aataggagct ataaattccc
    29461 aagacaatca caaattaaat caaggagata gtgaaaattt aacctgcgta atttcacata
    29521 aattaaataa aaactcctta cttgatgcat ttaaaaatag acatatattt tcaacggaat
    29581 caaaaacttt aaaaatgcta ttttctttaa attcaacctt tatgggggga acaataactg
    29641 tatattccgc ctcaaaaatt agtctctatt tacaagttga agataatata aacaaaatat
    29701 ctaaagttca atttttaact aaccttggaa aaatcattaa agaaattaaa gatataaatt
    29761 tgcataatgt taaatatatt tttgaaaaag aggtttcttt aaatgaaact tggtttgtgg
    29821 ctaaagttta tttaaataac aataaagagg ccatgtcttc tccaatcttt gttaattatg
    29881 aataaaaaaa caaactccat taataatgga gtttattttt ttgaatcctt attatgttta
    29941 actctatttg gttttgcctt tggtttaata acaacttttt tctctttttt aagctttgga
    30001 tatccatttc taagaatatc taagaaccat aagaataata tagttgctac tgcagaaatt
    30061 gctaacataa tccattgatt catatggatg aacataggaa taaggaagat tattattgct
    30121 gcaactaaaa ttatccattt attaatttta actttgttaa atacaaatct acttaataat
    30181 gaaaatcccc cgaaaatcac tactgctaaa agtattattt gtaaaattaa tacaaatatt
    30241 cccatagttt tgcctccttg cataatactc cttgtataat atattagtct aaaagaaaaa
    30301 cattttcaag tttttaattt ttaatgttgt tcttataaaa ttaacattta taatagcatt
    30361 tttattaatg tgttaaatta ataaaaatat atttgtgatt ttcaaaagta tatatagtat
    30421 ttgtataaaa aatttgataa aatagaatta tcattttatt caaattttaa cgaggtgata
    30481 ctatgtcttt aataaataca tcaactcatt tagatgaatt agatttacaa attttagata
    30541 tcctaattaa agattgcaga actccttact tagagatagc aagaatatgt cacgtttcag
    30601 gtggtacaat ccatgttaga atgaaaaaga tggaggattt aggaattata aaaggtacta
    30661 gaatactttt aaatttgcct aaattaggct atgatgttac atgtttcgtt ggtattcatg
    30721 ttaactcaac ttcatcatat actcatgttt ttgatgaact ttcaaaaatt aaagaagtag
    30781 ttgaaattca tttaataact gggggttatt ctatttttgc taaagttatt tgcaagaaca
    30841 tatcagacct tcaagatatt ttaatgaaca aaattaatca tattgaagga ataagcggaa
    30901 ctgatacatt tatatctcta tctcaaccaa tagatagaaa tataactctt taaaaaagaa
    30961 atagctaagc tatttctttt tttattttat ttttgaatat gaataaaatt caagcttcta
    31021 ctaataatat taacatatct tagaaaagga tgtgttaata atgaaaacat taaatattat
    31081 tgctcttact ttagtaataa taggtgctct aaattggggt ctaattggta tcatgaattt
    31141 taatttagta gattcaatct ttggaatagc ttccatttgg agtagaacaa tatatgtatt
    31201 agtaggatta gcaggattat actgcttatc attctacggt tatttcaatc gtataaaaga
    31261 ataaaaataa agctaaatcc ctattagaaa tctctaataa ggatttagct tttacatttt
    31321 ttcaattata ttaaaaagtt ctatggctgc ttttttaggt ccttcctttt caaggcctaa
    31381 aactcctaaa caagttttta tttgtccaac cttcacttta ttattaaaca tatcttcaaa
    31441 atatttatct attattaaat ccatctcttc tttcttttgc acaggtccaa aaggattagc
    31501 aaaatatgat tccactaagc cattttcagt agttgttccc tttgccattc tagcaccagc
    31561 tttaaaaatt ttcttagctt cctctggatt attaaaatat tcaatagatt tttctccctc
    31621 atctaatcca tcataattat tagcataaag gaaaagatct acatcatatc cccttattat
    31681 atcgtcatac ttacatactg gcataagaag tctagcattt atcttatcag gattcataaa
    31741 tatacttcta tcaatttctt taaaagcata cccttgatct aagtcatcaa gtcttacaaa
    31801 ggctcctatt tcagttccat accccttaat tatgccatct acatttttaa atacacccat
    31861 gtcatcaaaa attatggtca tatcgcttat atattcttca ctcagtgctc taaaggcttc
    31921 taagctttca gattttcctg ctccactatc acccattatt acaatattag cttcttttcc
    31981 atttttaaga accacattga ccatagctcc atgaataggc atatatcctc tttttatcat
    32041 tattatatta tgaagagtta atatcatctt tttcatatac ccaaaataat ctatttcttc
    32101 actataatta gcatacccta gcattatgtt atttttttca tcatcataga aacaagtttt
    32161 taactcatca tcatcatctt tagctccaaa gatataaata atatctggtt ttacccctct
    32221 acattcctct acagttgcta aatcaaacaa attacataaa cttattccat gagacataaa
    32281 atctctatgg aaatatacat atgctaataa ttcccctacc ttagcaggat aacaaaacca
    32341 ttcttcacta tttatatgag atccacttaa tggattttca cttgtttcta agaatatacc
    32401 atccctagta ttcttacttg gataagttat aaatggcgct tctaatgata ttgcatctat
    32461 aaatggaata tgttctaata tggaatattc ccttgaactt ggccatatta aattacttac
    32521 cataatactt gcatttccac cagcagaaac ttgtctaagt atattaggca tacttcctaa
    32581 taaatttttt tggagtctcc tgtaaaatct taaaattaaa tcattaaaat ttttattagc
    32641 atctataaat ccaactgctg caataccatc ttttatttta ttgttttgaa taacagtgta
    32701 tctttctaat tttctccaaa aagaataaac atcttctata atgtcagcaa aagatttttt
    32761 atcttcaagt aaaccttcaa aagcatcatt aacaactatc acctcatcag cccccatcat
    32821 agataatagt tttaatgcac ttattaaata cctagatata acctttatat cttcagtacc
    32881 tatggcttca cttaaaaatc taaaattatg actcttctta tttttagttt tttttaaata
    32941 agtttctaaa acttttataa acccttcgct atttattatg gattcaaaac tatcacaata
    33001 ctttgcagta aagttcatag ttaccttccc atgctttaag caaaattctt ttctcattaa
    33061 taagccccct tttacttttt acaagtttaa atatgaatat gtccaattca attatatcac
    33121 taaagttgta tccttccaaa atctttcatt atttatttta attgtaatta taaaatttac
    33181 aaattaaagt ttatttcttt aattatctgt taataatcca ttatatgatt aattattttc
    33241 aaaggagttt tttaattgaa aatcacctct aaaaaagtta gtatattttt aatatttata
    33301 ctattaatat ggatattttt atattctact tcatataaaa ttctaaatga aaattttaca
    33361 gtccattata tagatgtagg tcaaggagat tctgctttaa tccaaagcaa tggtaaaaat
    33421 cttttaatag atgcaggtac tcccgaatct actaagcctc tagtaaaata tttaaaaaac
    33481 cttggaatta aaaaacttga ttttataata gccactcatc cccatgcaga tcatattgga
    33541 ggtatggaat ctattattag aaaatttgat gtagactatt tttgtgcacc aaaagttaca
    33601 gaaaatactg atgtatttaa aggcatggta actgcactaa aagaaaagga tattaatata
    33661 aataccatat ctaaatcaaa atctcttcct atagatttag gtgagaacac taaaatagaa
    33721 gtctattctc cctctgataa taagtatgaa aacctaaata actactcacc tatccttaag
    33781 atatattatg gtgaaacctc ctttttgttt actggagatg cagagaatga agcagaaatg
    33841 gaagcagtta aatcaaataa aaatttaaaa tcagatgtgt taaaggtagg acatcatgga
    33901 agtaaaactt cttcaacaga ttccttttta aaagaagttt cccctaaaat agctgtaatt
    33961 tcttcaggac tagataattc atatggacat ccagcaaaag aaaccctaaa aaaattatct
    34021 tcctataaaa ctaagatttt tagaacagat gaagatggaa ctattatttt aacctctgat
    34081 ggaaaatcta taaaaaagca tagaaactaa aggcaccaat aattggtgcc tttagttttt
    34141 atagttttat ttactacaat aaactattaa tttttacttt ctttactttc actatcttct
    34201 aaattaacta atatcttttt ccaacttaat aatatgtaaa ataaatttac aacatcttgc
    34261 tcattataga caagtatctt ttcaactttt tcttttggca ttctttgaaa ataatcttta
    34321 tcctttaaaa ctttatggaa tgtttttgct aaatttgatc ctgaaagaac atccccctct
    34381 ctttcaattc caaatttctt ttctaaagct ttaagaccta tcccttgctt aaaagtatgc
    34441 tcataaattt tctgtatatc aacagatcta aagtaattat taaaatcaaa gtttatccca
    34501 tatttatcaa acaaataatt tataactgta aagtcattat ttccagaaaa agttactata
    34561 tatttcttct tcttctctgc tgcaaccatt ataaaatatt tctttgctaa gtaaagaata
    34621 tctctcactt ctcccttatg ctctatcatg tactgagtag taatgaattt tgatttctcc
    34681 tcatcaaaat atccagcacc aaaaactcct atgcactttg gtttcttata tacatagtgt
    34741 tctaaatcaa aatatacagc ctgtttatat atatcataat tttcattaaa agatagggca
    34801 ttactaagat ctaaatcatt tatatctatt tcatgctctt ttatgatcac tctatcactc
    34861 ttttcttata taaacttata ataaaaatat tatatcatta tatcattatt tcgttaagtg
    34921 ctaataaata ttaattaaat ttttaattaa tagaatttat caattgacaa tttccaggta
    34981 acttgatata atttaaattg atttgataat gattttcatt agtgaggtgc ttaatatgag
    35041 tatatatgat ttaaaaatag gccaagttgg tattgtagat aaaatatgcg gacacgatgg
    35101 tttagctaaa agacttcttg ccttaggctg catagaagga acagaagtta aacttaaaag
    35161 agttgctcca cttggcgatc cattaatagt aaatttaaga ggatttgatt tggcaatcag
    35221 aaagaaagat gctaaaaata taattttaaa ataataaata aaatagaata atttaattag
    35281 ggggaaaact aatgacaaca atagctttac tcggtaatcc taatgttggt aaaactactc
    35341 tttttaatgc cttaacaggt tctaaccaac atgtaggaaa ctggccaggt gttacagttg
    35401 ataaaaaaga aggatttttc aactctaaca aaatagtgga tttacctggt atatatgctt
    35461 tagatacctt ctcaaacgaa gaaaaagtat ctaaaaaatt cttagaatct ggagatgttg
    35521 atttaatatt aaatatagta gatgcttcta acttagatag aaacctatat ttaactatgc
    35581 aacttaaaga ctttaagaag ccaatagtat tacttcttaa catgaatgac atcgctgaga
    35641 aaaaaggaat taaaattgat cctaaaaaat tagaaaaggc ttttaatctt aaagtaattc
    35701 ctataacagc atctaaaaaa gaaggtttag atgaagttaa agaattctta aataaaggta
    35761 atttcgaaaa atatttagat aaaaatactt attcatttaa ggatgaggct gatacttata
    35821 aatttattga agataaatta tcagactgct ttaatagatc aagtaattct tcaactactt
    35881 taactgataa gttagataaa atatttttaa acccatggct tgcatatcca ttatttatag
    35941 cacttatgta ctttgtattc caatttacat tctcatgggt tggacaacct atttcagacg
    36001 ctttagatgg attcttaggt gatactttca tgccatactt aagtaaccta ttatcaggta
    36061 cggctccttg gttccaatca ttacttgttg atggtatcgt agccggtgta ggtggaatta
    36121 tagtcttatt accaataata ttagctttat tcttatgtat agcaatatta gaagatagtg
    36181 gatatatggc tagagttgct ttcttaatgg ataagttcat gagaaaaata ggactttcag
    36241 gaaaggcttt tcttcctatg ataacaggat ttggatgtac agttcctgct attatgtcag
    36301 ctagaacctt agaaagcgaa aaggatagaa aacttactgc tcttttagca ccatttatgt
    36361 catgtaatgc tagactacct gtatatgtaa tatttgctgg attattcttc ccagataatg
    36421 catctatggt tgttgcatct ttatacatat taggtatatt agtagctttt atattaggct
    36481 tagtatttaa aaatacttta ttcaagaaag atgaagaacc attcattata gaacttcctg
    36541 aatataaagt accagaagtt agatctgtat tagctcaact ttgggataaa tcaaagagct
    36601 tcttaaagaa agctggaact attatctttg caatgagtgt tgttatttgg ttccttcaaa
    36661 gctttaactt tactggaatg gttgaggata taactcatag tttcttatat aacataggtt
    36721 catttatagc tccaatattt gctccattag gatttggaga ttggcaagct tcagtttcat
    36781 tattaactgg tttaatggct aaagaaacta taatatcaac aatggaagtt atttataaag
    36841 gtgatttaac tcatatgtta cctcaattct ttacagcact ttcatcttac tcattcatgg
    36901 tatttgtatt actttataca ccatgtataa gtgttgtagg ggttatgaaa aaagaatatg
    36961 gtggtaagtt cacaatttta tcagtaatat tccagttcct agttgcttgg ataagtgcct
    37021 tcataattta tcatatcggt ttattaatct tttaaagaag gtgttttaaa tggaaattct
    37081 tattacagct ttaatcgtaa tagctgttgt gtttatatta tacaaaaact tcaaaaaatc
    37141 atctaaagga caatgtagtg gttgcagtgg atgtgcttca aaaggaagct gtcctagtag
    37201 aaaagataat aagaagtaaa aaaataacca attccaaatg gaattggtta ttttttatta
    37261 taaattacat tttttcaact acatctattc ctattaagaa taaagcattt ttaataactt
    37321 gagcagtagc ttttataagc tcaagtcttg cagcctttaa tccttcatct tctacattta
    37381 aaactgtatg attattgtag aatttattga aagccttagc tacttctatt gaatatctag
    37441 ttacaactga aggttctaac ttatctattc ctaaaattac agccttttgt aatccttcta
    37501 gagttttagc taattcaaac tcttcttttg aagttaactt tgtgaaatct ggctcagctg
    37561 ttatttcttg ttcctcagct tttcttaata tactcttagc tcttgcatat gagtattgaa
    37621 catatggacc agtttctcca tcaaagctta acatctcttt ccaatcaaaa actatatctc
    37681 tttctcttga gttctttaaa taagtaaata atatagctcc tatacctatt ttcttagcta
    37741 cttcctcttt attttctaat tgtgggttct tttcttctat agtttctctt gttttatcta
    37801 tagcttcgtt taataaatct tcaagtaaaa ctacattacc ttttcttgtt gaaagctttc
    37861 tatctgcgaa cttaactaat ccaaatggaa tgtgtacgca gtttttagcc cattcattac
    37921 ctgctaattc taatgttttg aatacttggt taaagtgtaa tatttgatct ttaccaacta
    37981 catatataca tttatcaaag ttatatgttt tatgtctata actagctgct gctaagtctc
    38041 ttgtagcata tattgatgct ccatctgatt taacaactat acaaggtggc atattgtatt
    38101 catctaacat tacaacctta gcaccattac tctcagttaa tatattagcc ttttctaatt
    38161 cttctactac tttatccatc ttatcattgt agaagctttc tccagcccaa gaatcgaaat
    38221 ctacgcctaa catatcatat atcttattaa attcttttaa agataagtct ttaaatcttt
    38281 cccatagagc tacagcttcc tgatctccat cttcaagctt cttaaagtac attcttcctt
    38341 catcttcaag ttctggatta ttttcagctt catcatggaa tttaacatat attcttaaaa
    38401 gctcatttat tggagcctct tctaaagctt cttcatttcc ccatctttta taagctgaga
    38461 ttaacttacc aaactgagtt ccccagtctc ctaagtgatt tattcttatt ggattatatc
    38521 cttcaaagtt taacattcta tataatgaat gtcctatagc tgttgtgaat aaatgtccaa
    38581 cgtggaaagg cttagctatg ttaggtgatg aatattcaac tacaacattt ttaccctctc
    38641 ctattttaga agaaccatat ttatctcctt cttctaaaac tttgcttatt atattttttg
    38701 agaaagctac tttatcaaca aagaagttta agtaaggtcc taaacactct actctttcaa
    38761 atccctcttt atttatcttt tcagctaatt cagcagctat catattagga gcttttctca
    38821 taacctttgc taattggaag caagggaatg caaaatctcc catttctggc ttaggtggta
    38881 tttctataag tttttctatg ttttctaatt ctagatcaac atattcttta attctttctg
    38941 caactaactt tttataatcc ataattgtcc tccttatttt atatcttttt aaaacatatc
    39001 ttataatgac attattgcca aaatataaat ttatttacaa tataaaaaac cgcctctttg
    39061 taacaaagag acggatatat ccgcggtacc actctaattg gttaaaaacc cactttataa
    39121 tttaacgcat aagcgacact ttaataaaaa tactagttat taaaatgcaa tctcagaggc
    39181 tctcttcatc taaactattc tataaggctt acaccatccc ttactcgctt aagaaatcat
    39241 aaagactact cttctcttca cagacttttt atattatatt ttcttaattt attatagtat
    39301 tttaccttaa accctgtcaa tatcctcttt catttattat gaataatatt ttgttttgaa
    39361 atatccattg aaaacttctt tgaaattcta tagtatcagt attataatca aattatattt
    39421 taaacaagta gaggggaaaa gaaatggata gaataggagt aatagacata ggctctaact
    39481 ctgtaagatt aacacttgct gaagttgaga agtctggata ttttaaaata attgatgaat
    39541 taaaagagca tataagactt ggctctgatt tcttcgcttg ctctgtttta gacgaagaga
    39601 aaattaagct tacaatctca tctataaaag cttttaaatc tatgtgcata tcttgtggtg
    39661 catcaaaaat tatacttata ggttcagaaa gcattaaaaa tgtagatgaa aaaattaata
    39721 ttaaagaaat aataaaaaaa gaacttggat tagaccttag aatattatct aatgaagaag
    39781 aaattcatta taacttttta ggagctaaaa attctcttta tatagataat tctcttcttt
    39841 tagatataaa cggaaataac actcacttat gttggttaaa aaacaacgaa atttataaag
    39901 aagttactct accctttgga tatgtaactt taacatcctt atttcaatta aacgacatga
    39961 ttggatatga aaataataca aactgtgtta attatattaa agaacaccta aaaaaaatag
    40021 attggcttaa agataattca tttaaatctg ttataggtgt tggaggttct ttcagaaacc
    40081 taggaaaaat tgatagacgt agaaaaagat atcctattga tatagttcat aattatgttc
    40141 tttatgacta tgatattgat gagatgtttc acctactaaa aagtaaaaat cttcgtcaga
    40201 gacaaaaaat tgaaggactt tctccatcta gagctgatgt catagttgct tctacagact
    40261 taataaaaga agttttaagc ttcacaaata ctaatgaagt aataatatgt ggtcgtggtc
    40321 ttagagaagg acttatatat gaatatataa tagataaatt ccatacttat gatgatattt
    40381 tagatataag tttaaaaggg attatggata ctttgaacat aaatattcct catgcgcaaa
    40441 atgtatgtaa acttgctttt tcactatttg aagggttaaa atcacttcat catttaggag
    40501 atgaatttaa aaatatatta aaaacagcct ccttacttca tgattgtggt ataagcataa
    40561 gatattatga tcaccacgta cactcatttt atattatttt aaactctcat ataagcggat
    40621 taactcataa agaaatatta ttaagtgcat tatgtgccgc ttaccataga aataactctt
    40681 tccaagctcc aattgctaaa tattcaacta taataaatag attagatgta aaacatgtag
    40741 agtatttagg agttattctt aagcttgctg aaggcttaga cagaggatta actggccatg
    40801 tagaaaatgt tgatgtgata attaatgaag atactgttaa tttaaaactt tattctgaat
    40861 atgatttaga attagagctt cgtcaagcta gaagaagttc taaagtcttt gaagaagttt
    40921 ataataaaaa tctagttatt gaaaaagcct aaataaaaaa gtttatggga aattattctc
    40981 ccataaactt tttttaactt acatccctaa atttatttaa gcttaaagca atctactttc
    41041 caatagactt tatttctcta ctatagattt agttgcttct aaagacttat ttaactgatt
    41101 taatgtttcc tgatctagtt tttttccttc ttcactattt aaagtctctt caactggtaa
    41161 tatataataa tatgattttt tattttctct tggaatttta tatacccaag ttgaagtgta
    41221 atcccatttc tctattttaa ccccttttct actcttatct aaggttaaat tcaatataac
    41281 accatcagct gttcttctat ctcccatagt ctctagtctt tgattagata taaaattacc
    41341 taaagaataa gctactaaag tcttatttcc atcttcaccc tctatttctt ctataggctg
    41401 tacaacatga ggattacttc ctaatattaa gtcaactcca tattcattta aggcttcagc
    41461 aatttttact tgatatttac taggttcagt tttatactct acaccccagt gcattccaaa
    41521 gactataaac tctgctcctt cattcttcat ctcttctact aaagctttca tctcctctag
    41581 ttccttatct aatttattat aagaaaaagt atttaaattc atatcttcag gaattttatg
    41641 accatttaac tctccctctt tgctttcata agtataagat gtaaatccta tttctattcc
    41701 ctttactttt ttaataatat acttcttatt cccaatagaa gtcatctctt tatctatttt
    41761 ttctccagtt ttactagctc cctctactcc cttatctaaa gaatgatccg ttgctacatt
    41821 tataatatca aatcccgtat cttttaaggc atctatcatg gaatctggcc cattataatt
    41881 aggatatcca gaatacccta tttcttctcc agcataagtt ccctcaattg ttccataggc
    41941 tatatcactt ttttctatga agttctttac atacttaaaa ttattcataa aatcataggc
    42001 cttattttct tcattatatg cagaccatat ctgttcatca tgtaatatta tgtctcctac
    42061 agcacttata ttaacctttc ttcctaagct aaacctatta aaaaatccct ttttatcatt
    42121 ctccttaatt acttcttttt catcaatact agctcccttt gttgttacaa cattattgct
    42181 tataatacta tttattacta aaacagaaca aacaataagt agagaggcta aaattacttt
    42241 aattttttta gacattctta tcatattctc acccctctat agattaccat atttttacat
    42301 ttcatttcaa ttatctttcc ataaaaaaat tagattagat attaaattcg aaaacaactt
    42361 ataaatcaag agttattttc aaattataaa cctaatctaa ttaaagtatt ataaaattca
    42421 aattatgatt aaataaataa acctatagaa tatacaaaag ccataaccaa ttgagatggt
    42481 atagctaata catatcttcc aagaagtatt ataactataa gtataggata ataatattga
    42541 tatactattc cagatagctt atagaataat tgaggtttaa ggtcctctaa aattctaaaa
    42601 caatcaagtc ctggaagtgg taatatatta aatacaaaaa agtttacatt taatattatt
    42661 atatattgta aaattaaaaa tataatttga gaaagatctc ctcttaaacc aaaaaatctt
    42721 aagtataaac ttgtaagtat tactccaatt atggcaacaa ctaagtttga aagccatgca
    42781 gctatgttaa ctttaagagc atccttcttt ggatttttaa atgcatattt atttatctct
    42841 acagatttac tccaaccaaa tccaaagaaa gccatcatta aggcaccagc tatatcaaca
    42901 tgctttaatg gatctaaggt taattcccct ttaaaccttg gtgatttatc tcctaattta
    42961 tctgccattt tagcacgagt atactctttt actgaaaaag ctattaatat tgctggaata
    43021 ctaagaaacg catctaataa aaattgcata atcaagacct ccttattttg tattttaaat
    43081 tataccataa atttcttgaa tttattattc agtaaactta ataatttatt aattttaatt
    43141 tttatatcag aatatttttc tacaaataag gttatatgtt tctctaaaca tttaaaaata
    43201 gaggttcctt tcctccctat ttaggagtaa gaaacctctt aaaattctaa atattataat
    43261 ctttttgcag cttcataagc caattcactt ctctcacatt cgtcatattt taaggtaact
    43321 tggtagcaaa gatgacttcc tttcatcttt tctgaagcat aacaaagtcc attacttctt
    43381 gaatctaaga aagcttgatc tatttgctct ggatctccaa ctagtattat ttttgatcct
    43441 tctccagctc ttgttataat tgctttaacc tgctttggtg ttaagttttg tgcttcatct
    43501 attataatcc aattctttat tatacttctt cctcttaaat aagccacagc ctctgttgtt
    43561 attatctttc tttcaaaaag ttcctctatt ttatcattta gctccctctc atttgaatat
    43621 ctttctttct catctgaatc tattaaaatc tctaaattat catatatagg cctcataaat
    43681 ggagctattt tttcttgctc ggttcctggc aaatacccta tttcttcatc cattgttaca
    43741 ttaggtctac aaactaaaat ccttctatat tgtccacttt cctcttctaa tatcttttgt
    43801 aatcccacag ctaatgaaaa taaagttttt gctgttcctg ctggcccctt tataattact
    43861 aaaggtgcct ttttagcatc catgctcaaa cattctagca taaatttttg gccaacattt
    43921 ctagggctta ttcccataat attattgtta tctttataaa gtaaaggtct tataactttt
    43981 ccatcaaatc ttcctaaggc agtttgttta tcattatcat tacagtgtat tattaaaaat
    44041 tgattaatct ctaattttgg ttccacatag cacttatttt cttcatcata gaaagttaaa
    44101 tcttttttct ccataacttt atttttaaag aatttactta agcactcaga acttgtgtaa
    44161 acttccattc ttccactata ttgttcttca aattctggaa ctacaacctc ataaaaatct
    44221 tctgatttta tgccaacagt atcagccttt attctctcaa atatatcctt agttattaaa
    44281 caaacatctt ctcccttttc ctttaaggcc ttacaaattt gtattattct attatcaggc
    44341 ttgcttatat tccatgcttc tggtatttca gtattataaa agttagtaac caccttaagc
    44401 tttccgcctt taggtaaatc aactccttcg cttaatgacc cacttaatct aagcttatct
    44461 aattctcttg caacgtgcct agcattagct cctaagtcat tattaatttt tttaatattg
    44521 tctaattcct ctaaaaccac ctctgggata ataacattgt tatcttcaaa tgaatatata
    44581 gctcctggcg aatatagcaa tacattagta tctaatatat aagttttctt caaaacaatc
    44641 actcctttag aataatacag ctataataat caaacttgat tttcatttgt ttatatatta
    44701 atatttaaga atactcaata taattcttaa atattgatat tttataatca aaatttctat
    44761 aatatataat atataaattt taaattagat tttattaata aaaattttag tttatactat
    44821 tatatggata ttagaatatg ggaggcctta aataatgaaa ttactttttt ttgatacaga
    44881 gactacagga gtcaaacccg gaagtatatg tcagcttagt tatattcttg taaatacttc
    44941 aactaaaccc caaaaaacaa taggtaaaaa tatatttttt acagttgatg aaatggaacc
    45001 agaagctgaa agaatccatg gtttttcctt agagaaatta tatgaattaa gtgaagggat
    45061 gtactttgaa gatctttatg ctgatttcat aaatgacttc ttagaagcag attttataat
    45121 aggtcataac gttcagtttg atattaaatt cttaaaacat gaattactag gtcttggaga
    45181 gttctttgaa cctaacaacg tattttgtac tatgaaatat tataaagata tttgtaaaat
    45241 attaagacct actggagatt ataaaaatcc taaacttgag gaagtaatca aacacttaaa
    45301 tataactgat tctgaaataa gtgaaactgc taataagtat ttccaaggta gtggaaacta
    45361 tcacgatgct agatttgata cagcagcaac ttatcttact gttataaacg gtataaaaaa
    45421 aggattaatt ccaagaaatt attttacaaa acttttagaa gcaaaataaa aagatatatt
    45481 ttcacaatat atcttttttt ctttactatg aaatctcttt ttcctttgct ttattaagaa
    45541 gatttatatt agaagtttgt atatttgtat aatatctaac ttctccactt tcactttcgt
    45601 atctactctc tactatttct ccacttacag agactaagtc tcctatgttc acaaatttct
    45661 ctaagaaatc cactgtagat ttactccaag taattacgct taaaaaggtc gttgtttctt
    45721 ctcttccatt aaaactattt acagctattc tgaatctaat atatttttta ttattcttag
    45781 attccttaac ttcaattttg gtagcaactc ttccaataag gtcaacttta ttcatgcttt
    45841 tcaactcctt gattatgatt tatctaaagt attgaccata tttaagagtc tcaaacatgt
    45901 gttctttaaa ttttttttaa caaattaatt taatataaca taaaaattca ttctttatta
    45961 taaatgttaa atattgtttt tatctttata attttttaag ccaaaagtta tatgaaactc
    46021 aaagctaaaa ttaataaata tttttatgaa atttttaatt ctttccataa aaaagagcta
    46081 ttaaagataa atctttaata gctctaaatt atattaaact gttatatcac tacttaagct
    46141 agctgcatcc ttattagctt ctattatagg gtttacatac tcatcaataa actctacaac
    46201 ttggcttgga gctctaccaa caaatttaac tggatctatt atagctataa tttcttcttt
    46261 agaaagatta aatgaaggat catttattat tctttttatt aaatcattat taagcccttc
    46321 tcctttaact ctttgagcag catccataga atgaactctt attctttcat gaagttcttg
    46381 tctatctcca ccttttttaa ctgattccat cattatattt tcagttgcca tgaaaggtaa
    46441 ttcttgattt acatgagctt caataacttt atcataaact accatattct cagcaatatt
    46501 tatataaaga tttaaaactc catctaaagc taagaaagct tctgctactg caattctctt
    46561 attagctgaa tcatctagtg ttctctcaaa ccattgagtt gaagccgtaa tagctggatt
    46621 taaggcatct actataacat atctagctaa tgctcccatt ctttcacttc tcataggatt
    46681 tctcttatat gccatagctg atgaccctat ttgatttttt tcaaatggtt cttctatttc
    46741 tttcatactt tgaagtaatc ttaagtcatt tgagaattta tatgcacttt gtgcaatttc
    46801 tgataatgta tttaaaatta tagaatctaa ttttcttgga taagtttgac ctgtaactcc
    46861 aaaactcttt ttaaatccca ttttttctgc aacgatttta tctaaagcct taaccttttc
    46921 ttcatcacct tcaaaaagat tcataaagct tgcttgagta ccagtagttc cttttacccc
    46981 tcttaatttt aaagttgata atagaaaatc tatgttctcc atatccatta ctaaatcttg
    47041 catccataat gtagctctct tacctacagt agtaagctgt gctggttgga aatgagtaaa
    47101 tcctagagta ggcatatcct tatattctaa agcaaatctt tttaaattat ttaaaactgc
    47161 aactattttt ttctttatta ataataatgc atctctcatt attattacat ctgtattatc
    47221 tccaacatag cagcttgttg ctcctaaatg tatgatacct tttgctgaag gacattgaag
    47281 tccatatgca taaacgtgac tcataacatc gtgtctaact tctttttctt tttttattgc
    47341 ttcttcgtaa tttatatctg aaatatgact tttaagttct tctatctgct cgtcagttat
    47401 atttaatcct aattcttttt caccctctgc aagagcaacc cataattttc tccatgttga
    47461 gaatttcata tcatctgaga aaatatatga catctctttt gacgcatatc ttgaattcaa
    47521 tggtgtgcta tataaatttt tcactttgta ccctcctaaa gtagaaatga taatttaaat
    47581 ttattatatc ataagataag tttattataa ataaatttat ctatttttta tctatcattc
    47641 gtgtttttac ctttattact cataaatttc ctacaaccta aactttaata tacgtatttt
    47701 atttatagtt tttgaaaaat cataaaaaat tattagaata aatttataaa aataaaagga
    47761 ttccactagg aatcctttta cgaagattac tcttctaaat tttcgattaa cttagctatt
    47821 tcttcagctg ctaaagcttc atcatcacca ctagcgataa ccttaacatt agcatcttta
    47881 gaaactccta atgataaaac tccaattaag ctcttaacat tagctttttt tccattgtac
    47941 tctaagctta tgtctgattt gaaagctgaa gcttttttta ctagtaaagt tgctggtcta
    48001 gcatgtaatc ctgttgaatt tttaactata acttctttag ttaccattat aaacacctct
    48061 ttacttttat ttgttattta ataatactaa cttcttaatt atactcattt ttttagaaaa
    48121 tttcaacaac ttaagaaaag attaatctct attcataagt ttattaacaa attcttttat
    48181 tctttttaat cccttaatta tttcctcttt agatgtagca taagataatc gtatgaagtt
    48241 atcttcccca aaagctattc caggtattac aacaacattt tcctctttta gtaatagatt
    48301 tgcaaattcc attgaaccct ttatattagc tttttttaga accttagaca catcaatcat
    48361 tacataaaag gcaccctttg gctctataaa agtaagatcc tcaatttcac ttataagctc
    48421 tatcataagc tctcttctta aactaaattc cttaaccata ttctttatag tttcttgatc
    48481 tccatttaag gcttctaaag cagcaaactg agctatacta tttgtatttg atgtcatatg
    48541 actctgaaca ttattcatta ccttaattag ctcttcgttg taacaagctg catatccaat
    48601 tctccatcca gtcattgcat aagccttaga aaatccattt ataactaccg ttctcttgaa
    48661 ggcatcttca cttgaggaag caatacttat atgctcctct tttccatata taagtttttc
    48721 atatatttca tcagaaatta ttataagatc attttcttta gcaaatttag caataacttc
    48781 taaatctttt tttgaatata ctgttcctgt tggattattt ggtgaattta ttactattgc
    48841 ctttgtattt tcacttaaaa ctgattttaa ttcgtcataa gtaaccttaa aatcattttc
    48901 tttttttgtt tctataaaaa caggaactcc atcacttaat ttaactagct caggataact
    48961 tacccaatat ggtactggta ttataacctc atctcctgga tttaaaatcg ccataaatac
    49021 attggctaaa gattgtttag ctcctgttga aataattata tttttagttc catagttaag
    49081 tccattatca tcatgtaatt ttcttgctat agcctctttt aactctacta ttccagatgt
    49141 ggctgtgtac tttgtctttc cttcttccat agctctagta gcagcttcta ttatattctt
    49201 aggtgtatta aaatccggct cacctactcc aaagcttact acatctattc cttctttttt
    49261 taattcacct gctttagctg ttaaggctaa agttagtgat gcccctatat tttgtgcttt
    49321 tctagataat atcatctaat cccctcttta ctgctttaaa tttatttatt acgcttccaa
    49381 tatatcattt ttattattta aagtaaacca ataaaaaata ctgctataag ttaaaatact
    49441 catagcagta tctatttatt taccaaattt agttcctcta taaactttat cttggttgta
    49501 ttataagctt tatagctgtt ctttcttcac catcaatttc gatgtctgtg aatgcaggaa
    49561 tacaaactat atcttttcca cttggagcca caaagcctct tgctatggct atagccttta
    49621 cagcttgatt tatagctcca gctcctactg cttgtatctc tacagtattt ctttctttta
    49681 ttatggctgc tagtgcgcct gctactgaat taggatttga tttagttgat acctttaata
    49741 cttccattat ataaacctcc tagacctttt taggatacgt ttacatttaa tagttaacgc
    49801 tttcccttac ctaataatat tctatataaa cgctctaatt ccttcttaaa tagataaaat
    49861 atttttaatc tttattttaa ataataagaa aactctactg aaatcattca gtagagttaa
    49921 ttaaaaaata cattttgtaa ccaaaatgta ttttacatat tcatttattt agcatagtca
    49981 actgcacggg tttctcttat tacatttatc ttgatttgac ctggatactc taattgttct
    50041 tctattttct taacaatatt tcttgccatt tcaattgccc ctgcatcgtc aacttgatct
    50101 ggtttaacca taattctaat ttctcttcca gcttgaatgg catatgactt ttctacacct
    50161 tcatatgaag tagctatttc ttctaatttt tctaatctct taatataagc ttctaaagtt
    50221 tctcttcttg cacctggtct agctgctgat atagcatcgg ctgcttgaac aagtactgct
    50281 tcaagagttt gcatttctgt atcaccatga tgggctgcta tagcatttac cacagctggt
    50341 gattcatgat atttctttgc aagttctcca cctataacag catgaggacc ctcatattct
    50401 tggtcaaccc ctttacctat atcatgtaaa agacctgctc ttctagctag atttacatct
    50461 aaacctaatt cagatgccat taatcctgct aaatatgaaa cctctattga atgttttaaa
    50521 acattttgac cataacttgt tctgtacttt agtcttccta gaagcttaat tatttctgga
    50581 tgtaatccat gaacaccagt ttcgaaagtt gcttgttctc cctcttcctt aatgtcattt
    50641 tctacatctt taatagctct ttctaccatt tcttcaattc tagctggatg aattcttcca
    50701 tctacaatta atttttctaa agctattcta gcaacttctc ttcttattgg atcgaagctt
    50761 gaaagaataa cagcttctgg agtatcatca attattaaat caactcctgt taaagtttct
    50821 aaagttctta tatttctacc ttctctacca atgattcttc ctttcatttc atcattaggt
    50881 aaggcaacaa catgtactgt agtttcagaa acatgatctg ctgcacatct ttgaatagca
    50941 ttagtaatta tttctcttga ttttttgtca gcttcttctt tggccttagt ctcaagttct
    51001 ttgatcagca tagctgattc atgttttatt tctcttctta cttcatctaa taataatgtt
    51061 cttgcatctt cacttgaaag atttgaaatt ctctcaagtt cagctctttg ttcaccgtat
    51121 aaactttgta cacgttcttc aagttcttga atctctatag attttttgtt tatgctatct
    51181 tctctttttt caagcatttc acctttttta tctaatgact cttccctttg gataagtctt
    51241 ctttcaaacc tttgaatctc atttcttcta tcacgaattt ctttatcaag atccgatctt
    51301 agtttgtgca cctcttcttt agcttctaat atggcttctt tctttgttga ttcagcttct
    51361 cttttagctt cttctagcgc taagctagct tctttttcca agctttccac cctatttttt
    51421 gaagcttttt ctataagagt gtattgaata aaccctacca ctcctaatat tattattcct
    51481 ataagtattc ctactaccat ataaacacct cctcgcttat tctctctata aaaacatttc
    51541 gcgtagaaag gtgtcatact attctcactg taattttttg aatatgataa accagtattt
    51601 gttattattg tatagtaatt ttaaacttat gtcaaggtta ctccttgcgt atattatatg
    51661 ttaaaaagct aaaattattc tcttataata ctagttatat tgtgcttttt ttactttttt
    51721 atattccaaa aaagctttaa aattttatta atgttaacat attcataaaa ctttaaattc
    51781 acacattaga attatcaaaa aattataaaa tcattaaaaa aagataagga tttccttatc
    51841 ttttttatta gctttcaaca gactcttctg tatttttttc tacacttgtt gattcaacag
    51901 ccttagctaa aggaagagaa tatttttctc ttatttgatt ttctatatct aaagcaactg
    51961 atggattctc ttttaaatat tgtttagcat tttctctacc ttgtcctaat cttatatctc
    52021 catatgagaa ccaagctcca cttttttgca ctatattttc ttttactcca acgtctacta
    52081 tattaccttc ttttgatata ccttcattgt acattatatc aaattcagct tgcttaaatg
    52141 gaggtgcaac cttattctta actattttaa ctcttgttct attacctgtt attccatcac
    52201 cttgttttat tgaatctatt cttcttatat ccattctaac tgaagcatag aattttaacg
    52261 ctctaccacc aggtgttgtc tctggattac caaacattat accaactttt tctcttaact
    52321 ggtttatgaa tattactacg cagtttgatt tgttaattgt tcccgttaac tttcttaagg
    52381 cctgtgacat taatctagct tgaagaccaa cgtgagaatc tcccatttca ccttcaattt
    52441 ctgctcttgg aactaaagca gcaactgagt ctacaactaa aacgtctata gctcctgatc
    52501 taactaaagc ttctgttatt tctaaagctt gttctcctgt atctggttgt gaaactacta
    52561 aatcatctat attaacacct aatctttttg cataaactgg atctaaagca tgctctgcat
    52621 ctatatatgc cgctgctcca cctaatttct gtgcctctgc tactacatgt aaagccactg
    52681 ttgttttacc agaactctct ggtccatata tctcaataat tcttcctttt ggtactccac
    52741 caattcctaa agctatatca agatctaagc atccagtaga aacagcatcc atttgaggag
    52801 ctccttgctc tccaagtttc attactgatc cctttccaaa ttgcttctct atttgaccca
    52861 tagccatctc aatagctttt aatttatctt tatctatatt tgccatacgt aacctcctta
    52921 aataaccgaa cataagttct actattatta taatatatac aaaaaaaaag tcaacatcaa
    52981 catgaagttc ttaaaagaac ctcatgtctt aataattgac tattttttga cttttaaaac
    53041 attatttata agtatttata acatttttat tttttctaaa gtaatctact ccagaaatta
    53101 ttgttattgc aacagctaaa tataagaata tatctggaac atatataaag aaatttctta
    53161 aaaagctgct acctgaaact aattccttaa ttcctggtga attaattata tttacttgta
    53221 ataataaaag tactatggca attatttgta ttacagtttt aatttttccc caccagctag
    53281 cagctataac taacccctct gatgcagcta tggttcttaa acctgaaaca gcaaactccc
    53341 tagctattat tacaatagct gcccatgcag gaactaattg taattcaact aaagatataa
    53401 gtgctgctgt aactaatagc ttatctgcca aagggtccat aaacttacca aagttagtta
    53461 cttgatttct tgatcttgca atatatccat ctagttgatc tgtaacagat gctattatga
    53521 atataagtgt tgctattaca ctcccataag gtaagtcttg gtaagctata aatactaaga
    53581 atatgggcac taaaattatt ctaattaaag ttaatttatt tgcaagattc attgcataca
    53641 actcctacca aatcatattc taaggccttt tcaattttaa ctttacagaa ctggtccttt
    53701 ttagctccat ttcctttcac ataaatacat ccatctatat ctggagccat ctcatagctt
    53761 cttcctatat aatattctcc attaaacttt tcgataagaa catcatattc tctacctaat
    53821 ttgtttttat taatttcttc tgataattct ttttgaagta acattatttc ttcttcccta
    53881 tcttttttaa cttcttcatc tatttgatct tccattatag cagcaggtgt tccttcttct
    53941 cttgagtaag taaatactcc aactttatct aattttattg tctttataaa atcttttagt
    54001 tcattgaaat cttcacaaga ttctcctggg aaaccaacta ttaaagaagt tcttaaaact
    54061 atattaggaa cgttttttct taaatcatta atttttccta ttatctcttc ttttgttgtt
    54121 tttctaccca ttctttttaa cacattattg ctaatatgtt gtataggtaa gtcaagataa
    54181 ttacatacct tatcatttac tgaaatctct tttataagtt cgtcatatat agcctctgga
    54241 taacaataaa gaactcttat ccactctatt ccttcgatct tagctaattc tcttaaaact
    54301 aagtgaagtt tcttttcacc atatatatct atgccataat tagtagtgtc ttgagctatt
    54361 aatattaact ctttcactcc attttcagct aattttttcg cttcttctac aatactttct
    54421 aaagctctac ttctaaactt acctcttatt ttaggaataa tgcaataagt acagaaatta
    54481 tcacaccctt ctgcaattct taagtatgca gtagctttat cagttgttat aagtcttaac
    54541 ccttcattaa ttgatacatc gctataattt gtagctttta ctttttcatt atttccattt
    54601 ataaagttca ttatggcttc atttatttta gcataatcat taacacctaa cattatgtct
    54661 atttcaggca ttaattctaa taattcatca ccatatcttt gagttaagca gcctgtagct
    54721 ataagtaact tacagttgtg acttgtttta tattttgcca tatctaatat tgtgtttatg
    54781 gattcttgtt tagccttttc tataaatccg caagtattta ctattattat gtcagcttcc
    54841 tttggattat tagtaagttc gtactcgttt tgaaccattc caagcatgat ttctgagtca
    54901 actctatttt tatcacaacc taaactcacc attcctacct tatatttcgc caaatctact
    54961 tcctcctacg tttaaatata tatttcttat tttaaaactc ttttttaaat taaacaagtt
    55021 tttattttat attttctaat tcatctttac taagaagcac ttgtcttggt ttacttccat
    55081 ctcttctaga aataacacca cattcttcta gctcttctat tattcttgcc gctctattaa
    55141 atcctattct taatcttctt tgtaaatatg atgctgaagc ttgaccactt tcaactacga
    55201 tttctatagc ttcatctaaa agttcatccc tatcaccatc accatttccc tctgaagcaa
    55261 tagttgctga atttatatgt tctaatatat cctcttcata ctgagcatcc ctttgtgact
    55321 cttttataaa tgatacaaca tgttcaacct cttcctctga aataaaggct ccttgaactc
    55381 tttgaggctt agactctcca actgggtaga ataacatatc tcctctacct aaaagttttt
    55441 cagctcctgc tgagtctaga attgttctag agtcaatttg gcttgaaact gcaaaggata
    55501 ttctagaagg tatattagcc ttaattaccc ctgtaattac atctacagat ggcctttgag
    55561 tagctattac taaatgcata cctgcggctc tagccatttg ggcaagtcta caaatataat
    55621 cttctacatc atgaggacaa gccatcataa ggtctgcaag ctcatctact ataataacta
    55681 tataaggaag cttttctgga acttctcctt tgttatataa agcattatat gattctatat
    55741 ttcttactcc attatctgca aaaagtttat atcttctagt catttcattt acagcccagt
    55801 ttaatgccgc agcagccttc ttaggatctg taactactgg gattaataag tgtggaattc
    55861 cattataaac atttaattca acaacttttg gatccaccat taaaagttta acttcatctg
    55921 gagaatactt atataatata cttactatta atgtgtttat acatacactc ttaccagaac
    55981 cagttgcccc tgcaataaga acgtgaggca tcttacttaa atctgtaaca acagccttgc
    56041 ctgttatgtc tttccctaaa gcacaagcta ccttaaattt attatctaaa aattctttag
    56101 attctacaat ttctctaaag aaaactggtg tttgttcttt atttggaacc tctattccta
    56161 tagcagattt tcctggaata ggtgcctcta ttctaactcc tttagctgct aatcctaaag
    56221 ctatatcatc agctaaatta actattttac taacctttat accagcctta ggttgaagtt
    56281 caaatctagt aacagatggc ccttttgtta cttgaagtat tttcgcctct acaccaaaac
    56341 tcataagagt ttcttcaagt ttagcagcat tagctaataa tgctttttta tcatctttat
    56401 ctaattcatt attattgttt aaatttaata aatctgcatt tggcgcaaca taactagccc
    56461 ctacacttga gcatttacta gctatattat ttgatatttc tttatctata gattcttctt
    56521 ccttagcttt ttctgagttg aatcctcttt gctctattat ggtgttatct aaacttaagt
    56581 ctctaggcat tttagactct tgtttaaaat catttatctt aatattatcc tttaattgat
    56641 tatctttttc cttcttatta tcttctatat catttaaaga agaatttttc ataaagtcta
    56701 atatctttat cttattgtta tcatctgaaa tatcatcttt tttactctct ttatttcctt
    56761 cagaaactcc atcttttttc tttaaattaa tataatcatc tttttcatac tttttatcca
    56821 caaatttaac cttagacgct ttttctttaa aacctccaaa gaaatttctt attgaaacta
    56881 aattataatc gaatataagc attgaagata taagatataa ggttacaaaa ataacaaaaa
    56941 gtcctatttt acctactaat ttatataatg gtaatacaat taaaaatcct actactcctc
    57001 catgccattc gctttgagaa taccatatag tttttataga tccccatata cttccctgaa
    57061 cataataatc gtttatattc acagtctgaa taagtaaaac actagttata agtgctatgg
    57121 ttattcctaa tcctattctt gaaaataaca cctctctttt aaataagaaa agctttgtac
    57181 ataaatatat tatgtaaatc ggaataaaaa aagctccaat tccaaaaagt cctattaaga
    57241 gagttcgtga tataacactt aaatatcctg ctttatcagt atataaacta aacataacaa
    57301 ttaaagaaac acatatcgct attaaggcat atatttcttc cgtaacttta ctttggcttt
    57361 gatttttctt tttttgagtt ttagttgcgg ttttcttttt tcccccagcc atactaaact
    57421 aaatcacctc accctatttt aatttcaaaa tggtaaaata cttgtaccaa aaaatattat
    57481 atacataaat gactataaat tgtcaaaata tttttatatt acataaatag aaccatagat
    57541 atactatggt tctacattat aagcttctat taagctattt aacttatcta aggcttcctt
    57601 aacaccgcca acttcgttta ttaatccgca cttaactgct tcttctccaa ctaatattgt
    57661 tcccatatca tttaaaagtt catctgtttg catcatcatc ttctttaata catctaattt
    57721 aatttctgat gtccttatta taaaatcatt tattctctct tgcattttat taaaataatt
    57781 aaatgtttga ggaaccccta ttacaagtcc attcattctt attggatgta ttatcattgt
    57841 agctgatgga gaaataaagg aataattaga tgcagttgct agaggaactc ctatggaatg
    57901 acctccccca ataactaagg atactgttgg tttttttagg cttcttatca tctcagcaat
    57961 tgctaaccct gcttcaacat cccctccaac agtatttaaa acaaataata taccttttat
    58021 tctatcattt tgttctatat caattagttg aggaatcata tgctcatact tagttgtttt
    58081 agtttgtggc ggttgaacac tatgtccttc aatttgccct attattgtta aaacctgtat
    58141 attagaatct tctttcttat gctcaggtaa aactgctccc atttctttta tttcttctat
    58201 acttttattt tcattttctt tgttatgttc catatatatc cctcctgtac taaaattatt
    58261 ttgtacagaa aggaaattta tatacattat ttcataaata ctctatgaag tgcttttata
    58321 gcttctctta catttttact ttcaacaaga caccaaattg tcatatgtga atctgctgtt
    58381 tgtaaaactt ctatgttttc attgtctaaa gcacctacta ttttagccat aactcctgga
    58441 attcctctca ttcttgaacc aactatagct atagtactta aatcttctac taaactatat
    58501 tttaaattat tttttctcat aatatcctca aataattctt taacacctgc atctattgta
    58561 aacacttttt ctttagggaa gatattgatt aagtctaaac ttattttatt gtttgcaaga
    58621 ctttctaaaa tagtcttata atttatatta tctttattgt catcagattt tatagaaact
    58681 tgaattctat ttccttgatg agttattcca gttataaact tattattatc tacatctcct
    58741 agactatcta tcattgtacc aatgcaagta ctcatagtat tctttattac taaggttaca
    58801 tttcccttca ttgctatttc tacagctctt ggatgaatta ccttagcccc ttgatctgct
    58861 aattggaata cttcattata acttatttta tttataagct cagcatctcc aactattctt
    58921 ggatcagcag tcattattcc atcaacatct gtatatattt caatttcttc agcctttaat
    58981 gcaactccta aaactgctgc cgtagtatca cttccgcctc ttcctaaagt agttaagaac
    59041 ccatttctgt caactccttg gaatccagca acaacaggca cttttccttc tttaagaact
    59101 tctaatatta atttaggatt tatatctata aaatcagcac ttgaataatt atcatctgta
    59161 agtattcctg catttcctcc tgttaaagga actgcttcta tttctgcctc tcttaattca
    59221 tcactcatga caacagtgct tataatttct ccacatccca taagtaaatc agtagctaat
    59281 ttattttctt ttttaaactc ttctgatact aatcctaaaa gtgtatcagt tgcataaggt
    59341 tctccttttc ttcccatagc agatacaaca acaactggta aatatccttt atttattgcc
    59401 ccttttacct tttctacaac catagttctt ctttcttttg ttgatacaga agtaccacca
    59461 aatttttgta ctactatttt caaaatcccc acactcctac agcttagttt tttttatcat
    59521 atcactatct acatctaata ttatggtttt agctcctatt ctttttacat tttcccactc
    59581 gatttctaag tattcatccg agttaaaaag attaaacttg ccaccaccta aatttactat
    59641 taaatatttt agctttcctt cttcatctat aataacatca ttatttataa gtccgctata
    59701 cttttcacca tcatttatgt ttataatttc atatctttct atttcgctta aatatctaac
    59761 attattactc ataaaacata cctccactta tttattgaaa caataaataa tatgttgatt
    59821 ataaataata tatgtcaatt ctcattaaaa gaggtaggct ttatgaataa gaaaaaaaat
    59881 aaaaaggcaa taataattgc cttttactat tgctgtttat tttcttcttc agcttctttt
    59941 attgcatctt ttcttgaaag atttattcta ccttgagaat ctatttctgt taccttaact
    60001 aatatttcat ctccaacaga tactatatcc tcaacttttt ctactctctt attatctaac
    60061 ttagaaatat gaactaatcc ttctttatta ggtaatacct caacaaaggc accaaattga
    60121 gcaatttttg taactttacc taaataaatt tctccagatt taacttcttt agttaaacct
    60181 tctattattt taacagcttc tttaacacct tctgattcag ctgatgaaac ataaactaat
    60241 ccatcttctt ttatatctat tttaacacca gtatcagcta taatcttgtt tataactttt
    60301 ccaccagccc caattacatc tctgattttc tcaggatcta tttgtagagt tgatgtctta
    60361 ggtgcatatt ttgaaacttc ttctcttgga gctggtatgc attctttaat cttatctaag
    60421 atcattaatc ttgcttttct cgctccatat atagcatcgt atataacttt ctctgataga
    60481 ccttttattt tagtatctac ttggattgaa gttattccct cttctgttcc agctacctta
    60541 aagtccatat ctccaaagaa atcttctaaa ccttggatat cagttaaaac ttcttcctca
    60601 gttaaatctt ttgaagttat taatcccata gctatacctg ctgctggtct ctttattgga
    60661 actccagcat ctaaaagtgc taatgtacta ccacaaactg aagcctgtga agtagatcca
    60721 tttgagctta atacttctga tactaatctt attgtgtatg gaaactcagc ttctgaaggt
    60781 ataagtggtt ctaaagctct ttcagctaaa gctccatgac caatttctct tctacctggt
    60841 cctcttaatg gtctaacttc tcctacactg tatgccggga agttatagtg atgcatatat
    60901 ctcttagatt cttcttcacc aataccatct aatatttgaa tttcacttat tgctcctaaa
    60961 gttgcaacag tcattacttg agtaagtcct cttgtaaata atcctgttcc atgagttctt
    61021 ggaagtaatc caacttcaca accaatgttt cttacctcat caaaagctct tccatcaggt
    61081 ctttttcctt cttttaaaag catatgtctt acgacttctt tttgcatatc ataaactgct
    61141 tctccaatat cagacatgtt atctggatat ttttcttcga actcttcatt tattttagct
    61201 ttaacttcgt ccattgcagc atttctttca tctctatcag taatatgcat tgactcacta
    61261 atcatatcaa aagcaaattc tcttacagct tcttctattt cttctggtac tttatgtagt
    61321 tctggcacct ttttctcttt accgaacata cttacagctt cttcttggaa ttttattata
    61381 tcttgacact tatcaaaacc aaatttgata gcattaatca ttgtatcttc tgggatttca
    61441 tctccaccag cttcaatcat cataactctt tccttagttg cgcaaacagt taattgaagt
    61501 gaactttctt ctctttcctt tgaagtagga tttagtatga attcttctcc tattaatcct
    61561 acttgaactg cagccactgg agtagtaaaa ggtatgcttg ataaacataa tgccattgag
    61621 gcagcattta tagctagtat ttcaggaagg ttatcatttt ctactgaaac aactgtacat
    61681 actacttgaa catcatttct atatcctttt gggaataaag gtcttaaagg tctgtctata
    61741 gctctaccat ttagtatagc tttctctgaa ggttttcctt ctctttttat aaatccacct
    61801 ggtattttac caactgaata taatctctct tcgtattcaa cacttagtgg gaaaaagtct
    61861 attccctctc ttggtttttc tgatgcattt acatttgtta atattactgt atcaccataa
    61921 ctcattaata tagcagcatc tgaaagcatt cctaatttcc cgaactcaac tttcattggt
    61981 cttcctgcta tgatagtttc atgggtatga ttcatataaa tacctccttt cggtatataa
    62041 agactaaatt tttacaatat taatattttt aaaataatag agcggtaaaa aacccgctct
    62101 actaaattat cttcttaatc ctaattcttt gattatagct ctgtatcttt cgatatcttg
    62161 ctcatataag tagtttaata aacctcttct ttgtccaacc atcattaaaa gacctcttct
    62221 tgagtggtgg tcttttttgt gtgttcttaa gtgatcagtt aaagagttta ttctttctgt
    62281 taataacgcg atttgaactt ctggtgaacc agtatctcct tcgtgtcttg catgtttagc
    62341 tattatttct tgttttctta ttttatccat tgttacacct ccgaaaaatt atcccctagt
    62401 tccaagaaaa aagacggtta cccttaaatc ttagagttag gttctatatg attatatcaa
    62461 aactctcttt tcttgtaaat ctttattaca aagaaatttt tatgttactt tcttccgcga
    62521 attttttatc cttaattagt tgttctttta attcttctaa agaattaaat tttttttcat
    62581 ttcttatctt acttataaag taaagtttta tatgctttcc atatatatct tcatcaaaat
    62641 ttagtatgtg agtctctact gtagtattct taccatttac agtaggatta tttccaactg
    62701 aagttatacc tttataaatt tctccatcaa cttctacatt agtataataa actcctatct
    62761 taggaaggat tatttcatca ttattcttta aatttgctgt tggaaaatta attgttctac
    62821 ctattttctt tccacttacc acttcaccat ttaatgagaa atacctacct aacatattat
    62881 tagcatcttc tagctctcct tctgataaag cttttcttat tcttgttgaa ctaactactt
    62941 ctgatttata tgtaaatgga tctataatat aaagttctaa tccctttaaa tcacataatt
    63001 ccttaagtaa cttaacatcc cctttatttt tatggccaaa tctatagtta aatccaacaa
    63061 caattcctcg tacattatag ttttttataa gattgtctat aaactcttct ggttctagtt
    63121 ccataaactt tttattaaac tcaacaaaac tacttaaatc aacttttaaa tcttcaagaa
    63181 ttcttatttt ttcatgtaaa gttactaaaa gttttggtgc acctgccttg tttataaatt
    63241 ttcttggatg atttttaaat gtatatacca tacttaatga atcgttcttc ctagacaatt
    63301 cattactttt gtttattaaa gctaaatgac ctttatgaat cccatcaaaa ctccctaaag
    63361 ctacataggt gcttttttga accactctct taaaatcatt atttattaaa atcattgact
    63421 actcctctat cagtaatttc tccaatttaa atcctttttc atcccttttt cctaatccca
    63481 aaaagactcc attgtcttca tatactctat atagtttatt aaattcaact tcttcactat
    63541 acattctatt atcaaatact ttaacaccat ttcttaaaag cttaccaaac ttctcattta
    63601 ctgtgatttt ttcaaaagaa tctaaagctt tttcaatgga aataatgtgt ttttctaaaa
    63661 gttcttctgt taaatcctct atatttattg cttcttcttt agtaaatgga ccattttgta
    63721 ttctctctaa ggcagtcatt gttgctccaa catttaattt ttcaccaata tcatagcaaa
    63781 ggcttcttat atatgttccc ttagaacaac aaacatccat ttgtatatta tcatttgatt
    63841 ctatctttat attttcaata gaataaatag taacttttct agcttctctg tgaacttcta
    63901 ttccttggcg tgctaattca tataatctta ctccattttg ttttaaagca gaatacattg
    63961 gtggaacttg atctattgtt cctaaaaagg aatttataca atttaatatt tcatcctttg
    64021 ttatatgtga tgcatcctct tctcttaaaa cttctccttc taaatcatat gtgtcagtaa
    64081 ccataccaag ctttaaattc actctataaa cttttttatt ttccattata taatctataa
    64141 tttttgtagc ttttccaagg catacaggta aaactccaga agccgctgga tctaaagttc
    64201 ctgtatgtcc tacctttttc atttttgcaa ctcttcttac catggccaca acatcaaatg
    64261 aagtcattcc cgtattttta tatatgttaa ttactccatt catatattac accatctctt
    64321 ttgcaagtat ttctaatatt ttatttttag cttcgtctat acttacacct ttaatgaatg
    64381 ctccagaagc ctttgtatga ccaccgccac caaagttttc tgcaattttt ctaacatcta
    64441 atgtatcttt agatcttaag ctaaccttta ctccctcatc tacctcttta agaagtatgc
    64501 atccttctac accttttatt tgatttccta aggatacaac atctgaacta ttttgaactt
    64561 cttctccaat agaatctatc atctctttag taattctcat aagaactatt ttattattgc
    64621 aaactagctc catatctcct aaaactaaag acattagttt cattttagca aaaggcttat
    64681 tatcaaatat atttctatga atttcatcat gttttatata attaactaat tcccctgcta
    64741 ttatatgagt tctttcagta gaatttgagt atctaaaaga acctgtatca gttattaatg
    64801 atgtatatag acatttagct atattttcat taatttcaac ctttaactcc ttaagtagca
    64861 taaatattat ttcagctgtt gcagaagcct ctgtatctac atagttaata acaccatatt
    64921 tatcatttga taaatgatga tccatattaa ctaaagttcc tttgaattcg cttaaatctg
    64981 cggaaattct ttcaacattt ccacaatcta atactataac acaatcagta tcttctgatg
    65041 gctttataac ttctccagtt atttcctcag cataaggcag aaagcttagg ttatctgaga
    65101 ctaggtcttt agagataaca taagcttctt taccataaat tctaatgcct tgaagtagtg
    65161 ctaagacact acctaaggca tcaccatctg gagatacatg atatgttata ccaatttttt
    65221 tgcttttatt tataattgat attacatcat gaaatctcat tttttatttc tccttgattt
    65281 tgtttagcaa tgattcaata tacattgcat tatctataga attatcttct tcaaataata
    65341 tttcaggagt acttcttagc ttaactctat ttccaacttc tcttcttatg aagccagctg
    65401 aactttttag agcctttaag ctttcctctt tttcttcatc atttttagcg aataggctaa
    65461 caaaaacttt agcgtatctt aaatctttag taacttcaac ttgagttaca cttaccatag
    65521 ctgttagtct agggtctttt atttgatttc ttatgatatc acttacttct tttcttactt
    65581 cttcatttat tcttttacct ctgaagttag ccatataaat cacctcttta taaaattaaa
    65641 gttcttttct ttgaacagct tccatagtat atgcttctat tatatcgcct tctttaatgt
    65701 cattgaactt ttccacacta aatccacact catatccagc atttacttct tttacatcat
    65761 ctttaaatct ctttaatgat gctaacttag attcaaatat tacaatacct tctcttataa
    65821 ctcttacatc tgcatttcta actaatttac cagttaatac atatcctcca gcaattgttc
    65881 caacgttaga aattttgtaa gtagctctta tttcagcagt tcctaataca acttctttgt
    65941 attctggatc taacatacca atcatagctg acttaatatc ttctatagcg tcgtatatta
    66001 ctctgtaagt tttaacttca actccatctc tctctgaagc tgctacggca ttgttatctg
    66061 gtctaacatt aaatcctatt actatagcgt tagatgcagc agctaatgtt atatctgttt
    66121 ctgatatagc accaacagct ccgtgtataa ctcttacctt tacatcatca gttgataatt
    66181 tttctaatga tgcttttata gcttcaacag aaccttgaac gtctgcttta actataactg
    66241 aaagctcttt aactgaacct tctttaattt ggctatataa atcttctaat gaaactctat
    66301 gtgaagttgc gaaactttct tgtcttattt tttcttttct agcttcagcc atgtttctag
    66361 cagtcttttc atctttaaca actgtgaatc tatctccagc agctggtact tctgaaagac
    66421 ctaaaacctc aactggaatt gatggaccag ctgattttat cttcttacct gagtcatcaa
    66481 acatagctct tattcttccg taagtactac caactatgat tgaatctccc atatgtagag
    66541 taccattttg aactaataat gttgctacag cccctctacc tttatctaat ttagcctcta
    66601 caactgttcc ttttgctttt cttccagcat ctgcttttaa ttcttgcatt tctgaagtta
    66661 aaagaaccat ttctaataga gtatctatgt tttctctagt tttagctgaa actggtactg
    66721 ctatgatatc tccaccccag tcttcaacaa ctaatccatg ctcagttaat tcttgtttaa
    66781 ctctatctgg attagcacct tctctatcca ttttgtttat agcaactatc attggaacgt
    66841 tagctgcctt acagtgattt atagcttcta ctgtttgtgg cattattccg tcatcagctg
    66901 caacaactaa gataactacg tctgttattt gagctcctct agctctcatt gctgtgaaag
    66961 cttcatgtcc tggtgtatct aagaatgtta aagtttctcc gtttacttca acagtgtaag
    67021 cacctatatg ttgagttatt ccaccagctt ctgtacttgt tacctttgat tttcttatag
    67081 catcaagtaa tgaagtttta ccatggtcaa cgtgtcccat aactgttata ataggtggtc
    67141 tcttagctag gttttcttct tctacatcag tttcttcttc gaactcttct aaagtattct
    67201 cttcttcttt ttcataaact gcaacctcat atttttcagc aactttttca gctgtttcaa
    67261 aatctatttc ttggtttata gcagccatta ctcctgtgaa gataagagtt tttataacat
    67321 cattaactgg tttgtttaat ttctcagcta attctttaac tgttatagtt ttccctattt
    67381 cgataacttc aacgtcttgt tctaagctat catcttcgct accttttttt cttttctttt
    67441 tcttcttagc agcattatta acttcttcag ctaattcagc ttcatattca gcaaccaagt
    67501 cactgtttgc ttcacttcct gctaaaagtt cttttattaa tgcagcatct tcatcttcta
    67561 taacgctcat atggttttta acctcaacgc caaactcatc cattaatacg tttataagaa
    67621 ctttacttgg aactcttaac tcttttgcca attcataaac tcttattttt gacatatatt
    67681 cacccccgaa aaatttttag atgaactaat tattattaga atatgacaaa agtttttctg
    67741 ccatattctc atcagttact cctaatatat tgatttcgca tctacctata ctatatccta
    67801 actcttcttt tgaaaaatta tctatgtatg gtatatcata ctgttcacag tacttaacga
    67861 atttatcttt tgaacgattt gaaatgctat ttgagaaaat aaataagtat atcttacttc
    67921 ttttcatttc ctcttcacat ttattataac cctccaaaag tttacctgct ctttttgtaa
    67981 gtcctaaaaa attaagaaat ttatccattt gttatctcat ccctcaatct atcatagact
    68041 tcatctccaa tatttgattg aagatttcta tttaatcttt ttgctttaaa tgctttttct
    68101 aaacattcaa cacttttaca aatgtaagcc cctcttcctg actttttacc agttaaatct
    68161 acagaaactt ctccttcagc atttttaact attcttatca gttctttttt aggcttcatt
    68221 tccatgcaac cattacacat tctcattgga atttttttag gtttcatctt gtcacctcca
    68281 aagtctagaa gttattattc ttctattata atgtcttctt caacaacaat atcttcttct
    68341 tgtaataatt cttctgcttt agatttgctc tttatgtcta ttttccaacc agttaattta
    68401 gctgctaatc taacgttttg tccttcttta cctatagcta atgataattg actatcatct
    68461 actataacct tagctgattt agtttcttca tcggcttcta cacttacaac cttagctggg
    68521 cttaaagcat tttctatata ctcagatgga gtattactcc attttattat atcaattttt
    68581 tcatttttta gttcattaac tatattttga actctaacac ccttaggtcc aacacaagca
    68641 cccatagcat ctactgattc atcatttgag taaacagcta ttttagttct tgatccagct
    68701 tctctagcta tactttttat ttcaactatt ccttcatata tttctggaac ttctaattca
    68761 aataatcttt taactaaacc tggatgtgtt cttgaacata aaactgaagc acctttagat
    68821 gttttcttaa cttcaactac atataactgt aatttttcat tgaagttata tttttctgta
    68881 ggcatttgtt cgtttggtcc taaaacacct tctatttttc ctaagtctac aaatacatta
    68941 cctttatctt ttcttattac tgtaccaggt aatatgtcaa attctttttc tgcaaattct
    69001 gagtaaatta catttctctc agattctttt attctttgga taaccatttg cttagcaagt
    69061 tgtgctgcaa ctcttccaaa gtttttaggt gttacttcta tttctactat atcatctaat
    69121 tgataaatag cactaatagc ctttgcatct tctaaactta tttctgttac atcatcataa
    69181 acttcttcaa caacaacttt ttgagcataa acatggattt ctcctgattt ttcatcacgt
    69241 tttaccttta cattttgtgc agttgttcct gaatttgaaa agtttttctt ataagctgct
    69301 attatagcat catctatagt ttctaataaa gcctctactg aaataccttt ctcttttact
    69361 atttcagata atgcccctag aaattcttca ttcattttta aaccctcctt aaaattatat
    69421 ctccccttta aggttcgctg tcttaatttt atctttagga acttttattt cattcccttc
    69481 acattcaact attatttcat tttcttgtac atctttaaga attcctttta cattttttct
    69541 tccacttaaa gaactcttaa atccaacaaa aacttctttt cctataaatc tattaaaatg
    69601 ctcatcacta tgtaatcttc tatttaatcc tggtgatgaa acttctaaga aatacgcatc
    69661 ttttattgga tcttctatat caagcatatc acttaatgct ctacttacaa tttcacaatc
    69721 ccttagagaa attctctcct ctggcttttc tatatataat cttaaataat attcattatt
    69781 ttcttttaca tattcaatat gatataaatc atatcccttt tctttaacta taggagcaca
    69841 aagtgcttca agatcagcta caagttgttc ttttttcatt atacttccct cccttattaa
    69901 gttttaaatc ttatctttat tgtatccaat aaagcaaata cctattaatt tatataataa
    69961 taggtatgca aaaacgcaac tttattctaa gttgcgttac aaatagaaag agcgggcttt
    70021 tctgtcccac tcctttacct caaactatta gtaaaaaact tctacttata tagtaacata
    70081 gtatatttta tattacaaga aatttttaaa attagccaaa caaactaagt tggttacttt
    70141 cttgcattcc ttctaggcat ccatgattat ctaaagtttc tattacagtc tttgaaacct
    70201 tagatctaat tcttaaatct tcctttgaaa taaattctcc attaactcta cattcaacta
    70261 tactttttgc agcattatct ccaactcctt gaagcgcatt taatggaggt cttataccat
    70321 cttcttctat cttaaactta gttgcttctg atttatataa atcaaccttt aagaaattca
    70381 atcctctttt atacatttca taagaaattt ctaaaatagt taaaagccct ttttctttta
    70441 ctgatatgtt atttcctaaa gaatttagct cttccatttt tgcttttatt gcttcttcac
    70501 ccttacaaat taaatctgca tcgaaatcat cagctcttac tgtaaagtat gttgtgtaat
    70561 atgcttctgg ataataaact ttataatatg ctattctaac tgccatcata acatatgcaa
    70621 cggcatgacc tttagggaac atgtatttta ttcttttaca agattcaata taccaatctg
    70681 gcacattttt ttctctcatt aaagcttcat catcttcact aagtcctttt ccctttctta
    70741 ccttttccat tatagtaaag gctttcttag gcggaagatc tttatacata agataaacca
    70801 taatatcgtc tcttgttgct atacagtctt ttagtgtagt gtaaccttct ttaataaagt
    70861 actgagcatt attaagccaa acatcagtac catgtgaaag tccagatatt ctaacaaggt
    70921 ctgcaaaact cttaggttga gtatcaacta gcatctgtct aacgaattta gtaccgaact
    70981 caggaagtcc ataactacca acttcacatc ctaattcttc tttagttacc cctaaggcat
    71041 ctggtgatgt aaataaactt ataaccttag gatcatttaa aggtattgtc ttaggatcta
    71101 agccagttaa gtcttgaagc attctaagaa cggtaggatc gtcgtgtcca agtatatcaa
    71161 gtttaagaag tctaccactt attgaatggt aatcaaagtg agtggttata atatctgtgt
    71221 taacatcatc agctggatgt tgtattggac aaaaattata tatttcattg tcatttggaa
    71281 caaccatgat tccaccagga tgctgtccac tagttctttt tattccagta catccaatag
    71341 taagcctttc tatttcagct tgagatacaa ctaatcctct ttcttgaaga tattttttta
    71401 caaaaccata agctgttttt tcagctactg tacctatggt tcccgcttta aatacatatc
    71461 cttttccgaa aagaacctct gtgtacttat gtacaacagc ttggtattct ccagagaagt
    71521 taaggtctat atctggttct ttatcccctt caaagcctaa gaatgtttca aaaggaatat
    71581 catggccatc cttaatatac ttagctccac aatcaggaca atttttatct ggtaaatctg
    71641 ctccagaact tatagaacca tctaagaaaa attctgattt cttacaattt ggacaaacat
    71701 aatgaggtgg taatccatta acctctgtta tatctgacat tgttgctacg aaagatgaac
    71761 caacagatcc tcttgaacca actaggtatc catcttctaa ggatttagca actaattttt
    71821 gagctataag ataaagcacc gcatatccat tatttattat agaattaagt tctttatcta
    71881 atctcttttg aacaacttca ggaagatttt ctccatatat actatgaacc ttattcatag
    71941 tcatgtttct tatttcctcc tcagccccct ctattttagg agggaatgtt tcatctggta
    72001 taggttttac agcttcaacc atatcagcta tcttctgagt attttctatt acaacttccc
    72061 tgcaagcttc ttctcctaaa tattcaaact ctttcatcat ttcattagta gttctaaaat
    72121 ataaaggtgg ttgattatct gcatctccat atccttgacc agccattatt attcttctaa
    72181 atacttcatc attaggatct aagaaatgac aatcacatgt agcaactact ggtttatcca
    72241 tttcttttcc taaatcatat atttttctat ttaactctct aagctcttct tcatccttta
    72301 catttccttt tcttataagg aattcattat tttgtattgg ctgaatttct aggtagtcat
    72361 aaaaagatgc tatttctttt aattccccat ctgtcttgcc ttctaaaacc gccttatata
    72421 attgccctgc ttcacaagct gaaccaatta ttaatccttc tctatactca gttattatac
    72481 ttttaggaag tcttggcctt ctagcaaagt aatctaagtg agccatagaa actaatttat
    72541 ataaattctt aattccagtt tgatttttag ctaatattat tatgtgatgt agaggaagtt
    72601 tctttacatc tatatttgat aaatactttt catttagagt tttaacatca tgaatatcca
    72661 tttcttcttt catctttttg aaagaaaact ttaatatctc cgcagtagcc tttgcatcat
    72721 caacggctct atggtgattt tcaaggctaa ttcctaaatg tttagcaact agatttaact
    72781 taaccttttt taaatctgga taaagatatc tagctaaagg aacagtatcc attacaggat
    72841 tctttaaagt ttttcccata tccattaagt tcttttttat aaaaccaacg tcaaaggcag
    72901 cattatgggc tactaataca gaatttccta caaactccat aaatttagga agcacttcat
    72961 ctatagtagg ctgtccatta accatatttt gagttatact tgtaagctct gtaatcttat
    73021 atggaatatt tacctttgga tcaacaaaag tactaaatga atctactatt tctccatcct
    73081 ttaacttaac agcaccaatt tctataatct tatcattttt acttgaaaac cctgtggttt
    73141 ctatatcaaa aaccacatac tcatcatcaa aggattcctc atgcccatta ataactatag
    73201 gtgttccatt atccgctaaa tatccctcta ccccataaat aacttttatg ttattctttt
    73261 tagcagctat ttgagcatct ggaaaggcct gaacaactcc atggtctgtt atggctacag
    73321 ccttatgtcc aaactttgct gctctctcaa caattttact tgcagcagtt acggcatcca
    73381 ttgagctcat agttgtatgc atatgaagtt ctactctttt ttcctctgct gtatccatac
    73441 gctctatttt tgtaagctta tttatatccc tagccattat tacaacttcc ttagcataag
    73501 gatccatagt agcttctcct cttactttac aataaagacc ttttttaaca ttttctaaaa
    73561 catgttcttt atccttatct cttaagaagc atttaacggc tatggaactt gtatagtctg
    73621 ttataaagaa tgtaagtatt atcctacctg tcttagtttc aaaaatatca gttttaaata
    73681 catcccctga aatagtaaca attccagagc ccatatctat actgcttata tctatagtat
    73741 ctccttgaat atttcttcca agtatagtat tttcattctt aggctcatct ttataagatt
    73801 ttttctcata accgccttta ttgccaccat tagatttatg tttattatcg ctactttcct
    73861 tcttaacagg cttttcttga gcagcttggg ctttcatagt ttctataact tttttagcta
    73921 tttctttgtt ttcatttaac ttttcatcat tgtaattctt attagctaaa ctttcatcat
    73981 atttaacttc tactaaagaa tttattccaa agaatgattt tatatatccc ttaatctctc
    74041 tatctatatg tttcttatta acaaaagtac ataaaaactc agaaccattt tttataatta
    74101 ttttttctcc ttcaattact ataggagaat taagtaaaac ttcttttaaa acaggatgtt
    74161 tctctataat tttttcacaa acatgactcc aatgctgatc tactacctgt tttaaagtta
    74221 tatcagaaat atctatgtaa tacataatct ctatatttat ttcaaaacct atagcttttt
    74281 ttatatattg cttaattatt ttcttttgtt cttctgataa ttgatcttta ctttttatta
    74341 tggttcttaa agtattgctt ttcttcaaca attgaaactt agttatttca aactctaaat
    74401 cattaagatt atcatctgat tttatggatc tgttaatctg tttaacaaac tcgttactca
    74461 aaatctaagc ctcctttgaa ttttatttta atttatccct atacgacata aagaagggat
    74521 attaatccct cctttaaatt atattgtttc tattatcttt ataagctctt caactaagtc
    74581 ttcttctttt acttttttaa ttatttcacc ttttctaaag ataagacctt cacctttacc
    74641 tccagctatt ccaatatctg cctctcttgc ttctcctggt ccatttacaa cacagcccat
    74701 tactgcaacc tttatgtttt tcttgcaaga acttaatctt tcttctactt ctttagctat
    74761 gtttattaag tctatttgag ttcttccaca tgtaggacat gatataaact ctattccttc
    74821 ttttacatat ccaaagtttt taagaatttc tttacctact tttatctctt caacaggatc
    74881 tccagttaaa gaaactctta tagtatcacc tattccttct gcaagtaaag ttcctattcc
    74941 tatagcagat tttattgttc ctctccaagg tgtaccagcc tcagtaactc ctaagtgaag
    75001 aggataattt gttttttgtg aagctattct ataactttct atcatagttt caacatttga
    75061 tgatttcatt gaaattacta tatcatgaaa atctaactct tctaatattt taacatgttc
    75121 taatgcactt tcaactaggg catctggggt aggttttccg tattttgcta aaagctcttt
    75181 tgataatgac cctgagttaa cccctattct tataggaata tttttttctt tacaagcttt
    75241 tactacagct tctacctttt ctctagatcc aatatttcct ggatttattc ttattgcaga
    75301 aactccattt tctattgctt ttaaagctaa tctataatca aaatgaatgt ctgcaactag
    75361 tggtatatta atttttgcca ctatatcttt tatagcgcaa gctgcatcca tatctaaaac
    75421 tgcacatcta acaatatcac atccagcttc ttgaagctct cttatttgtt taactgtaga
    75481 ttctacatct tttgtatctg tattggtcat agattgaatg gaaactctaa aatctcctcc
    75541 aacatatatg tttccaacct taacctttct agtttctttt ctattcacta ttaattcctc
    75601 caaatttcta tagtatcggg aatattatat cttttatagt tactaatacc ataagtccca
    75661 taaggagcat gaatccaacg gtatttacag cttcaatgta cttagcaggt atttcttttc
    75721 taattatcat ttgaataagc tctataaata ttcttccacc atctaaagct gggaatggta
    75781 aaaggttaaa tactgctaat tgaacactta aaaatgccat aaaccataaa agagtatttg
    75841 ctcctgcttt tgctgcttgc cctgacattt taattattgt aacaggccct cctacatcag
    75901 tttttaaatt tgcttctcct gtaacaatag tctttaatgc aataaatgac tgagatacaa
    75961 gacttcttgt ttcattgaat ccttgcttta ttccttgaag caaagtaggt ttttcaacta
    76021 aagcatagct tattccaact tgatacattc cactctcatt taaaatcggc tgtactgttt
    76081 ttgttaagac atcattgcct ctttttatct ctaattcaac tggatttccc ttagctaaag
    76141 ctaatcccat tctaaaatca tcagttgtat gaattttgtt tccatctatt tttaggaatt
    76201 catctccctg tttaattcct atttcttgtg ctggtgaatt aggtactaca cttgctactt
    76261 tattttctgc aaagccagaa ctcatagcta gcccaataaa tattataagg gctaatacat
    76321 aattcataaa tgcccctgct cccataacaa gtattctttg aataggagtt tttgcattta
    76381 agcttcttga atcatcactt tcatcctctt caccaagcat tttaacaaat ccaccaaaag
    76441 gaaggattct taagttatat tctgtttctc caacttttac accaaaaagc tttggaccca
    76501 taccaatagc aaattcctca acataaatcc catttaattt agccacaata aaatgtccaa
    76561 gctcatgaac taagattaaa gcactaaatg ctaataaagc aaaaattata tacacccttt
    76621 tccctcctag ttatattttt catatatata ctgtcttact tctttatcta aatttattag
    76681 attatcaaga gtaacctctt tgttgtaatc aaaagtattc atacattctt ctattatctc
    76741 tggtatttgc agatatgtta tctttttatt taggaatagt tcaacacata cttcatttgc
    76801 accatttaag attgttggca taagctttcc cttttctcct gcttcataag ctaattttaa
    76861 gcatttaaat gtatctaaat ccggcttttc aaaagttaac tctctaatct taaataaatc
    76921 taacttttca ctaatatttc cttccctatt cggataattt aaagcatatt gaattggtaa
    76981 tttcatatct ggtacaccta attgagctat aactgctcca tcagtatatt gaaccataga
    77041 atgtattatt gattgtggat gaactacaac ctctatatct ttataagggc aatcaaataa
    77101 ccaatgagct tctataactt ctaatccctt gttcattaga gttgatgaat ctattgatat
    77161 tttttgcccc atattccact ttggatgctt aagagctctt tctggagtta ctgattttaa
    77221 atcttctata gtaaatcctc taaaaggccc tccagaagca gttaaaagta ttttatcaat
    77281 tttgttatga gcatttcctt gtaatgattg gaatatagca ctatgttctg aatctactgg
    77341 aagtattttc actccatttt ctttagcttc tctcataaca agttcacctg ctactactaa
    77401 agtttcttta tttgctaaag cgatatcttt tccttttctt atagcttcta cagttggttt
    77461 taatccaatc ataccaacaa cagaagtaac aactatatca atttcatcta aagtagcaac
    77521 tttaactaag ccttccatac cacagataac ttcacacttt aacccttcgc tctttactaa
    77581 atctacaagt tttttgtaag actcctcgtg atttattgca acgtatttag gtgaaaactc
    77641 ttttattata tccattgtta attctatatt tctattagct gttataccaa ctaattcaaa
    77701 atcttcttta tgaaatctta atacatctaa agtttgagtt cctatagaac cagttacccc
    77761 tagtatagat attctcttca tattctttcc tcctaagttc ttccttaaac ataatagact
    77821 aaaattatct acttagccta ttatattatt ttatatcatt atatctctaa atacaataat
    77881 atacatttat tatgttttct tttaacagaa agttatctac tcattgataa atctttttct
    77941 ctaaattata tttttcttta aaactattcc cacctttata aatatctcta cataataaat
    78001 atactcaagt tattacttaa tattctatga ataatcttat atttttcttt ttaagttaaa
    78061 agtataataa aatttttatg cctacaaata aatataaata aaatttttta taaaaaaatg
    78121 ctttagaaaa tctaaagcat taaaatttta agacaagtgt taaatagaag aatactattg
    78181 ttccagcaaa taatatactg tcaaatctat ctaatattcc accgtgtcct ggtattaaat
    78241 tactataatc ttttattctt gcgtatctct ttattgatga agcaactaaa tcaccaaatt
    78301 gacagaatac tccacataat atacctatta taaaataatg atatacaggt attaaagata
    78361 aactattaga tattattata ccaaatattc cacaagctaa tgtacttcct aatattcctc
    78421 ctatagaacc ttctatagtc ttcttaggac taacctttgg acataatttg tgtttaccaa
    78481 agaatctacc tgaatagtat gcagcagtat cacaaagcca tgatgatagg aatattaacc
    78541 aaactaggta ttgtccacct tctttgatat ttactaaatg tatgaagcta aagaaaactc
    78601 ctacatatat aaatcctaaa atagtaactg caacatctat aaatgtatat tcaatattaa
    78661 gtataggaac acaaagtaat ataaatgtag ctattattat taaaaatgat aacatttcaa
    78721 aattatttga agttaagtaa tataatatta ataatatata tccccctata gaaataggtt
    78781 ttaattttgc tactttaaca gttttataat attcatataa tcctacaaaa gaaagagcaa
    78841 tagtaaatag cttaagccaa attccaccta ggaatataaa agcaataaat ggagccatta
    78901 taagtgcgcc tagatatctg ctattagatt tcacccaaat cacctcatta tttatttaat
    78961 tccaccaaat cttctatctc tattttgata atcaaaaata gcttttctta aatcttcacc
    79021 attaaaatct ggccagttta tttgagaata ccaaaattct gagtatgcac attgccataa
    79081 taagaagttg ctaagtcttt gttctccact tggtcttatt ataagatctg gatctggcat
    79141 tcctttagta taaagatagt cattaaacat atcttcgttt atatcatcta tacttatttt
    79201 gcccgcttta acatcttcta ttacagattt tatacctcta actatttcat ttcttccacc
    79261 ataatttaat gctaagctta aaactactcc agtgttatct ttagtttttt ccatagccca
    79321 aagtatagct tttttaggtt cttctgggaa tcttgataaa tcacctatag tttgtaccct
    79381 tactccattt tgatgaagtt catcaatttc attttttata aattcaacaa ctaacttcat
    79441 aagagcatta acttcatctt ctggtctttt ccagttttca gtagagaaag catataaagt
    79501 taaatatttc actcctaatt tatcgcattc ttttacaata gtttttatag tcttaacccc
    79561 agctctatgt cccatagttc ttggaagacc tctttttttt gcccatcttc cattaccatc
    79621 cattattatg gcaatatgct ttggcatttt atcttcaatt agaactattt cttctgaatc
    79681 ttcatttcct tttttactaa aaaaatctaa catagaaggt tcctccttat aattcaatta
    79741 taaaataaaa aacctgctaa agagcaggtt ttttaaatta aattgacata atctcttttt
    79801 ctttagctgc cactatagta tctatttctt taacaaactt atctgttact ttttggatgt
    79861 catcttcacc ttttttcatt tcatcttcag ttatagttcc ttcttttttg aagtttttaa
    79921 ctttatcatt tgcatctttt cttatagatc ttatagcaac cttagcttgc tctccaactt
    79981 ttttaacgtt cttaactaaa ttttttctag tttcttcagt taattctgga actaaaagtc
    80041 ttattatagt accatcattg ttagggttta ttcctaaatc tgattttaag atagcttttt
    80101 ctatttcttt aagcattggt ttttcccaag gtgttataac taataatctt ggttctggtg
    80161 atgaaacatt agctacttga tttaaaggac acatgcttcc ataatactca acttgaattc
    80221 tatctaacat agttgggtta gctcttccag ctttcattgt agctaattct gaatttaaag
    80281 aatttatagt tttattcatt ttttcttctg atttttttat aacgtcctta atcatttaaa
    80341 actcctcctt attttttgct tactattgtt cctatatttt ctcctaaaac aactttcttg
    80401 atgttttcag gattatctaa agcaaatact aatataggga tgttgttatc catacaaagt
    80461 gatgttgcag ttgaatccat tacttgtaat ccttgctcaa gtacttctat atatgataat
    80521 tcatcatatt ttttagcatc atcgtattta tgtggatctt tatcataaac tccatcaacc
    80581 tttttagcta ataatattac atcagcttcg atttcagcgg ctcttaatgc tgctgctgta
    80641 tcagttgaaa aataagggtt tcctgttcct gctccgaata ttactactct acctttttct
    80701 aagtgtctca tagctcttct tcttatatat ggctctgcta tttccttcat ttctatagca
    80761 gtttgtactc tagtgtctac tcctaaagct tctaatgaat cttgaagtgc taaagcattt
    80821 attgaagtag ctagcattcc catatagtca gcagtagttc tatccatgcc ttctccactt
    80881 ctacctctcc atatgtttcc gccacctaca actgcgccta cttctatccc catatcaaca
    80941 agctctttta cagcctttgc tatgttcatt gctatattaa aatctattcc aaaaccattt
    81001 tcgcctgcta aagcctcacc tgataactta agcataactc ttttgtattt acaagttccc
    81061 atcttaaacc tccaaaacgc tgaattctct atttttgtta attttttata tctatattct
    81121 aaaaaaagag aacacaatgt gttctcttat gttattattt tccgcccatt ttagctactt
    81181 cttcagcgaa gttttcttct ttcttttcga ttccttctcc tctttcgaat cttacgaact
    81241 tagttatagt tattggagaa cctacttctt ttgatttttc ttctaataat ttagcgattg
    81301 atttgtcgcc atcttttacc caagcttgat ctaaaagaca aacttcttta cagtattttt
    81361 tgattcttcc cattaccatc ttatcaacga tgtgctctgg tttaccttcg ttaagagctt
    81421 gagctctgta tatttctttt tctttttcta aagattcttg atcaacatca gcttctgata
    81481 agaatagtgg gttagcagca gctatttgca tacaaacatc tttagcaact tcagctaata
    81541 ctggtgatga agcttcacat gctaattcag ccataacacc tattcttccg ccaccgtgga
    81601 tgtagctttg aactaatccg ctttcaacag aaaatctttc gaatcttctt acgctcatgt
    81661 tttcaccgat cttagcgatt aactcagtta aaacttcttg aacagttttg tctccgtcta
    81721 atttttctgc taataattct tctacagtgt ttacatttga gttagcaact aattctgcta
    81781 ctcttcctgc aaatccaacg aattcttcgt ttaaagcaac gaagtcagtt tcacagttga
    81841 tttcaactat tgaacctttt ttcatatctt ctgatacgta agttttaacg ataccttcag
    81901 ctgctactct tccagctttt ttagctgcag ctgctaatcc tttttctctt aaaacttcaa
    81961 ctgctttttc catatctcca ttagtttcag ttagcgcttt tttacaatcc atcattcctg
    82021 cgccagttct ttctcttaac tctttaactg ctttagctgt tatcattgaa attcctcctg
    82081 actatttaat taaaaggtaa acgaagcaga gagcttcatc taccttttca gtatttatct
    82141 taatattact ctgctaattg ctcgccttgt cttccttcga ttatagcgtc tgccatttta
    82201 ccagctatta atttaactgc tcttatagca tcgtcatttc ctggaattac gtagtcaact
    82261 tcttctggat cacagttagt atcaactata gcaactactg ggattcctaa tattttagct
    82321 tctgctattg cattcttttc ttttcttggg tcaacaacga acatagctcc tatgtttgaa
    82381 gcgtctaatt ctttgattcc gcctaagttt ctttgtaatt tttctctttc aagttttaac
    82441 ttgattactt ctttcttagg taaaacgtcg aatgttccgt cttgttccat ttcatctaat
    82501 ttgtttaatc tgtttattct tgtttttata gttgtgaagt tagttaacat tcctcctaac
    82561 catctgtggt taacgaagta catgtttgat ctttcagctt cttctttaac agcttcttga
    82621 gcttgttttt tagttccaac gaatattacg tctttaccag cttctgatac ttctcttata
    82681 aaattgtaag cttcttcagc tttctttaca gttttttgta aatcgattat gtatataccg
    82741 tttctttctg taaagatgta aggagccatt ttagggttcc atcttcttgt ttgatgtccg
    82801 aaatgaacac cagcttctaa taattgtttc attgaaatta ctgacattat tttttcctcc
    82861 tagattggtt atttcctcca ctatcttctt tactataagc actacttaaa gtagcaccgg
    82921 cttatagatt cgataatgtg tgtatttgtt acctttgata gtataacata gggttataaa
    82981 agtatcaaca agatttttca aagtttttat aaaataatat tatatcacta ttaaactttt
    83041 cacctaatta gatttcatct taaattatct ataaatatac tgaaaaagac tacttaatta
    83101 taaaaattta cttatttcta ttaaacttct tataaaagtt ataaatcata gattttttat
    83161 atttatttaa ttattattag cttgttatac aaaaaagaag aagatgtaaa aaacaccttc
    83221 ttcaaatctt attttatctt tttaagttca tccattaact tttcgtttaa tatttttata
    83281 tgagtacctt tcattccaag acttcttgac tcaataactc ctgcactctc aaattttcta
    83341 agagcattta ctataactga tctagttatt ccaactttat cagctatctt agaagctact
    83401 aataatcctt catctccatt taactcacta aaaatatgtt cgacagcctc taattctgag
    83461 taagataaag ttccaatagc taattgaacc actgcttttt tacgtgcttc ttcttctatt
    83521 tcaccttgtc tagctcttag aagctccatc ccaactattg tagcactata ttctgctaaa
    83581 actaggtcat catcattaaa ttcttcccca aacctagcta ataataaagt ccctaatcta
    83641 tctccattac caactatagg aacaatagtt gatatttttt tagagattaa acattcgcct
    83701 actccttcaa aaacacatat ccctttatta ggtatgtttg caattgtatc ttgaatgttt
    83761 attaatttat tattgtagtc ttctgggaat tttctatcaa caacaacttt ttctttcatt
    83821 atgtcacatt caaatttttc agaaaaagaa taacctaata ttctaccttt tcttccaact
    83881 aaatatacat tacatgacat aacatcactt aataattggc atatatctcc aaaagctata
    83941 gcttcagtac ctgatttttg taaaatctta tttaatcttc tagttttact aagtagtgtc
    84001 gacattatat cctcctcgca ctattttaca aaaaatgtaa atttcagaat ttttaaaata
    84061 ttcttataat aaacttatta tttaaatatt aacatagtaa atacttcatt tcaaccagta
    84121 ataatggtgg agataaggac taaaaactac ttttctgagt tattttcatc attatttttt
    84181 tcaattattt tttcatattt acattcttta tcgctacact gtaaatattc acctttactc
    84241 ttagagtatc tcttaaccat atatgaacta cattctggac atttttcatt tgttggctca
    84301 taccaactta cgaaattaca ctctggataa ttactacatc catagaatct gtttcccttt
    84361 ttacttctct taactaaaat ctttcctcca cataatggac agtttgcatc aacctcttca
    84421 actataggtt ttgtgttttg acactctgga tatccagggc aagctaagaa atctccaaat
    84481 cttccatgct taataaccat aagtcttcca catttatcac aaggtacatc actgacttta
    84541 tcttctataa taaccttaga tatctctttt tctgcctttt ctatatcttc ttttaatgga
    84601 gtaaagaatt catctactat atgagtccaa ctttcttttc cagcttcaac attatcaagc
    84661 ttaacttcca tttctgcagt aaagtcagta tcaactatct gcttaaaata atttgaaagt
    84721 atatcattta ctataaatcc taattctgtt ggtataagaa tctttttctc tctatctaca
    84781 tattttctac ttattaatgt agatattgtt ggaacgtaag tacttggtct tccaatacct
    84841 ttttcctcta aaagcttaac aaaagcagcc tctgaatatc tagcaggtgg ttgagtaaaa
    84901 tgttgtttac cttcaattga ttcttctttt aaaacttcat tttcttctaa ttcaggtaaa
    84961 agtactgatt cttcctcttc tcctgatata tactcataaa ctttcatgaa accatcaaat
    85021 tttatggtag atccactagc tcttaatgta taatcaccat ttttaatctc taaactatta
    85081 gtatttaata tacatgtagc catttggctt gctatgaatc ttttccatat taaagcatat
    85141 aacttatatt gatcattact taagtttgtc ttagcaatct ctggtgttat ttcaacatat
    85201 gtaggtctta tagcttcatg ggcatcttgt atatttttct ttcctttata tactcttggc
    85261 tcctctggaa catactcttt tccatatgtt tcatctataa agttaagagc tttttcttga
    85321 gcttcctttg agattctaac agaatcagtt ctcatataag ttattaaacc cacagttcca
    85381 aatttcttaa cttcaacccc ttcataaagt tgttgagcta ctgacattgt tttctttgtc
    85441 ataaagttaa gctttttact tgcctcttgt tgaagagtac ttgtagtaaa tggagctaaa
    85501 ggatttttat tcttagttcc tttttttatc ttgcttacta tatattcatt ttctttaagt
    85561 tcatctatta tttgatctgc ttgttcttta ttttttattt ctatcttttt cttattttta
    85621 gttgttaact taacaggaaa agacttcttt ccttttttaa gtttaacatc aacagtccaa
    85681 tactcctctg gattgaactt ctttatttct tcttctctat cacatataag ttttaatgct
    85741 gctgactgaa cccttccagc actcaatccc cattttacat tcctccatag gagtggactt
    85801 atttcatatc caaccaatct gtctaacact cttcttgctt gctgagcatc tactaaatta
    85861 caatttattt ttcttggatg ctttattgaa tttttaactg catcctttgt tatttcatta
    85921 aattctattc tacaattctc gttttcatct atcttaagca cattagctaa gtgccaagat
    85981 atagcttccc cttctctatc agggtcagtt gcaaggtata tcttatcact ttttttagct
    86041 aactttctaa gtttgcttaa aagttcacct tttcctctta ttgttatata tttaggatta
    86101 tagtcatttt ctatatcaac gcctaattgg ctttttggca aatctcttac atgtcccatt
    86161 gaagcttcga ctacataatt tttacctaaa tatttttcta ttgtcttggc tttagctggt
    86221 gactcaacaa ttactaattt ttgacccatc gtaaactctg aattaaatcc agagtgttca
    86281 ccccctcaaa ctatcatatt atttttgtat aataatctcc tggtataact tctattttat
    86341 tttcaaattg caattcacat aataactcat atatgataga tgtgtcaata tttgttactc
    86401 ttataatatc gtctatatgt agaatattat tctctaatat gcttaaaagc aactggttaa
    86461 tctctgaatt ttcctctttt ttgccatctt tattacattt tccttctttt tttattttaa
    86521 aatgtattga atttaataaa tcacctatat tagtaaatat gccagctcca tcttttataa
    86581 gattattaca gcccttgctc tgagaagaaa acacatctcc aggtactgca aaaacatctt
    86641 ttccttgatt tatagcataa tttgccgtta ttaaagatcc acttttttct ccagcttgaa
    86701 ccactataac agcttcagat aatccactta ttattctatt cctctgagga aaattatatc
    86761 taagaggtgg cgtatcaggg aaaaattcac taattataca tccattttta attatttttt
    86821 tatacaattc tctattatag acaggataat ctaaatttat tccacaacca aaaacggcaa
    86881 cattataaaa attttcttca acacactttt tatgtgcttc agtatcaact ccaaaagctc
    86941 caccgcttac tattccaaca cctgcttttc ctagccctga agctataaga cttgcacatc
    87001 ttttcccata tgaatcacat tttcttgatc caacaatggc aacattggta tcttcagctc
    87061 tttctaaatc tcccttataa aaaagaatat agggaggttc atctatattt ttaagtcttt
    87121 taggatagtc tttatctaaa tatgttacta ttttatagtc tttcttattt atatcattaa
    87181 ttaccctact attcaaatca atgttaatat catttttaaa tttactagcg catttaattc
    87241 cagtttcctc tatatccttt ctatgtatgt atatattttc ttcatttcct aattgattta
    87301 aaagctctac ttttatattg tttgaaatct ttaaacttcc aagccatatt ttataattta
    87361 aatcaacctc tttcatttat ataacctctc cctctaaatt ttttctataa ttaagtgcct
    87421 cataaatatt gcttttttta atatcttcac tgccattcat atctgctatg gttctagata
    87481 attttataat tctatctaaa gccctactac ttaaagaaaa tcttgtgtat atattgttta
    87541 aaacttcatg acattcttca tctaatctta ttaaactttg aatatgacta tgtttcatct
    87601 gagaattata agttatttct aaatctttaa acctaaattt ttgtctttct cttgcttcct
    87661 taattttatt tcttatatcc tctaaatttt cttttttatt acttgaatta agttcattat
    87721 aagaggcata tctaacagaa gtaaaaatat ctattctatc tagcatagcc ttagaaagtc
    87781 tatttctgta tttaatccgt tcatattcac tacaagtaca tattcttcct tcataaccac
    87841 ttaaataatt tccacaagga catggattaa aagctcctat aagcatgact tctgctggat
    87901 atgttacact tcctacattc ctagatatgg ttatctctcc attttccata ggctctctta
    87961 aacattctaa ggttcttcta tcaaactcta aaagttcatc taaaaacaaa ataccattat
    88021 gagctaaggt aacttctcct acccttaaat ctcttcctcc acctattaaa gatacccttg
    88081 ttgcagtatg atgtggattt ctaaatggtc tttcgatttt tcctttagta ttatttaaaa
    88141 gtcctgaaac actatatatc cttgttgtct ctaatatttc ttttttattt aattctggca
    88201 ataaagattt aactccctta gcaagcattg ttttacctga tcctggaggt ccttgcaata
    88261 taacattatg atttccagcc gctgcaatta tcatagctct ctttgcactt tcttgaccac
    88321 aaatctcatt aaaagaatta tctttcttta atgcacaaat ttcctcttct cttttataag
    88381 gtagcacatc tctataagtt aaaaatccaa ccacctcttt taaattagag aaaccatata
    88441 aattaatatt atcaacacaa gaacattctc ttaaattatc tattggtata ataaatttat
    88501 cattgttatt ttcaactcca gatataacag agcacaaagc tccattaaca gatctaatat
    88561 ggccatctaa agataattct cctaaataaa cagtattctc taaaatctcc ctttgaattt
    88621 gaccactctc aattaatata gctaaagcta taggaagatc aaatccagtt cctacctttc
    88681 ttaaattacc tggagctaaa tttactgtta ttctccctaa aggaaattca taaccactat
    88741 ttaaaatagc cgatcttact ctctccttag cctctctaac tgaagtatcg gctaatccta
    88801 ccaaagtaaa acttggcaat cccttactta tatcaacctc tatattaaca attacacctt
    88861 ctattcctat taaagttgaa cttttcaaac ttatagacat acaaatcacc ttctttaaaa
    88921 gatatacttt caaaaatcat actaaccaca aaaaaatttt ttaatcatta aaattttaaa
    88981 atgtttaaac ttatacttaa gtgttaaaaa ttcagtagag gtgatttaaa tgaaaaaata
    89041 taataaatct ataggtttct acggggagaa tttaagcgca aaattcttag aaaaagatgg
    89101 ctattcaatc ttagaaaaaa actttaattg ttcatctgga gaaattgata ttatagctat
    89161 taaagatgaa ataatttctt ttatagaagt aaaaagcagg ttttctgact cctttggtaa
    89221 gcccaaagaa tcagtaacct gctctaaaca aggtagaata ataaatgcag caaaatacta
    89281 tctacatata aaaaaactct acaattatta tattagattt gatgttattg aaattaactt
    89341 tcatattgat agttcaaagt atgaattaag ctttttaaaa gatgctttta gagtatagct
    89401 atagaatatt ctttaaaaaa ctcattctat gaatttctgt aggccctact tcttttaaag
    89461 catctacatg agtcttggtt ccataaccaa catttttttc aaaatcatat tgtggatatt
    89521 ttttatgata ttccttcata atattatctc tataaacttt tgcaattatt gaagcacagg
    89581 ctatacaagc actttttgta tcacctttta taacatgctt attttctcca ttaaagtttt
    89641 ttattaaata tccatctgaa aggactaaat caggctttat attaagtcct tcacaagctt
    89701 ttatgaatac atgattatta cagtatccta tgcctttttc atctatttcc ttgctatcac
    89761 acattgaaat agaataactt aaggcttttt cttttatgat ctcacttaac tcttctcttt
    89821 tatgttctga taattttttt gagtcattta tgtaaagaat tatatcatct aaattactag
    89881 aatctaatat tactgctgct gaaactatgg gacctgctaa aggtcctctt ccaacttcat
    89941 caactccagc tacatactta taatcaccaa aactcttatc aaaaaaatac atgtttttaa
    90001 ctctttcctc ttctttaata agattattta gatttttctc taatctattt cctaaagatg
    90061 atacattttt tcttttatcc tcttttaata agtcagcaag ttcttttatt tgagctttgt
    90121 tatctctatt tacatctaac tcttttacta aatcacttac tactttaaaa gagtatgaat
    90181 ttaaattttc tctcatatct tttattaaat tatccataat aaaccctact tcctattctg
    90241 gtttttctaa agatattttt cctatttttc cgcctctaaa ttcatctaaa attattcctg
    90301 caattctatt gtaatctatt tgatttcctg ccattatagc tcctcttttt ctagctatag
    90361 catctaaatt atctaatgga ttttcatgaa gttctgtaag tttatatcta gtcattaatt
    90421 cttctggatt tgtttcttga agtctttcaa ctagtttaag agctaattct tcagtatcca
    90481 ttatttcgtc ttttatagct ccagtaaacg ctaaatttaa tccaacaatc tcatcttcaa
    90541 atttaggcca taaaactcct ggtgtatcca ttaattctat gcctaactta gtttttatcc
    90601 attgtttatt cttagtaaca cctggtctat ctccagtttt agctatattg tttttagcca
    90661 ttttatttat aaatgttgat ttaccaacgt taggaattcc aacaaccata gctctagtag
    90721 tgattttagc taaaccttta gcttttaatc tatcatgttt ctcttttaaa agttttaaaa
    90781 gagcaggttt tatagcattt aatccttcac cttttaaagc gtttacttct aaaacaattg
    90841 tttcatcatt agttaagcta tctttccaag ctttagttac cttaggatca gttaagtcac
    90901 tcttatttaa aagtattact ctaggtttac cagcacatag agtgtctata tctggatttt
    90961 tagaagattt aggtattcta gcatctctaa tttctataac agcatctaca agctttaaat
    91021 tgttctgtat ttctctttta gttttgacca tatggccagg gaaccagttt attgccataa
    91081 taaatcaccc ttccattttt tattttaggt tatattcaat atattatctt aaacaaatta
    91141 aatcaatatt gtttaaagta aaattctaat atttttatat aaacaaaaaa tgggactatt
    91201 cactagtccc agtttatgtt taaattatct agttaactct ttaactttag cagctttacc
    91261 tactctgtct cttaagtaga ataatttagc tcttcttact ttacctcttc ttacaacatc
    91321 aatcttatcg atgattggag catttattgg gaaagttctt tcaacaccta caccgtaagc
    91381 aactcttctt actgtgaaag tttctcttaa tccaccgttt tgttttttga taacagttcc
    91441 ttcgaatact tggattcttt ctctgttacc ttctttaatt ctaacgtgaa cttttatagt
    91501 gtcacctatg ttgaattgag ttaaatcttc tctgatttgt tctttttcga ttgctcttat
    91561 gatttcgttc attgtgcatt cctccttgat atcttctaaa attttgacgt tcttaacaca
    91621 ctctgtgaca gaggaccgcc ggtactagca cgaagtttat tctatcatac ttgtattctt
    91681 taatcaatat atttattttg attttaacaa ttttatatct tcttttgaaa gtttaatttt
    91741 ttcaaacata tctggtcttc tttccttagt tataagaagt gattcttttc ttctccactt
    91801 tcttatattt tcgtgatgac ctgatataag aacctctgga actttctctc cttcaaattc
    91861 aaaaggtctt gtgtattgtg gatactctaa ggtcccatca taataacttt catcttggaa
    91921 actctcttca cttcctaaaa cgcctggcac taatctagat atgctatcaa taactggaat
    91981 acatgccatt tctccacctg ttaacacaaa atctccaaga gaaatttcta aatcaaaata
    92041 tttataaatt ctttgatcta taccttcata gtgtccacaa acaaaaataa gctcttcctc
    92101 tttagaaagt tccttagcca tttcttgatt gaaagtttta cctcttggtc ctaaaaatat
    92161 aaccttaccc ttattttttt ctttaacagc ctttatagca tctacaattg gctgaggagt
    92221 cataaccatt cctgctccgc caccataagg ataatcatct accttcttat gcttatttaa
    92281 agtataatct cttatattta ccgtctctat atgtagaaaa ccattttccc tagctcttcc
    92341 tattatgcta tgtttaaata tatcaaacat ctctgggaac aatgtaagaa tattaatctt
    92401 catattgcca ccctccagaa ggtcttatag tgattaattt ttcatccata ttaatatcta
    92461 aaactatatc ttttaatact ggaactaata cttccttctt tccttgaacc caataaacat
    92521 cattacttgg agtttggatt acatctttga tttttccata ctcaaatcct tctgtatcaa
    92581 caacagtgca tccaactaaa tcaactatga aatattcatc ttcttcaagc tctatagcat
    92641 cttcacgttc tatttctaaa taaattcttt ttagtttttc agcttcctct attgaattta
    92701 ttccttctaa tttaagtata actttatcct taaagtattt tacgccctct actttaatct
    92761 tttgcccttt taagataaca tattctaaat agtcaaatct tctcatatct tcagttaaag
    92821 gcataacttt catttcacct cttaagccat gagtgtttat tatttgacca actactaata
    92881 aatcttccat tattacaccc cttttctgta tataaaataa gagttaggta ttcccctaac
    92941 tctttttaaa tgatttctac tacaactttt ttattttctt taattgcagc agctttcact
    93001 actgttctga tagctttggc aattctacct tgctttccaa taacttttcc catatcttca
    93061 ggtgatacct ttagttctaa tatgattgat tcatctccta agatctcatt tacatgaaca
    93121 tcctcaggtt tatctactag tgacttagca attatttcaa ctaattcctt catattattt
    93181 ctacttaaaa ataagtagat tcacccccta ggaaaactat tttataccag cttggttgaa
    93241 taatttttta actatatcag ttggttgtgc accatttcct aaccacttag cagccttttc
    93301 agcgtctatt ttaacttcag cttctggctt agcgattggg ttgtagtatc ctatttcttc
    93361 gatgaatctt ccgtctcttg gacttcttga atcagcaaca actactctgt agaaaggagc
    93421 tttgtgagca cccattcttc ttaatcttat tttaactgcc attacatttt cacctccttc
    93481 aaaagtgtgt tcaatttatt tcataaaagg aagtttactt aaaaatccct ttttactaac
    93541 atttttttgg aatgatttca tttgcttcat ttgctttttc atcatttcat agccttttaa
    93601 gactttattt acttcttgta ctgttgtacc agaaccttta gctattcttc tctttcttga
    93661 accatttcct attactaaag aaggttgttt tctttcttta gctgtcatcg attgtatgat
    93721 tgctttaact gtagccattt gtttttctcc ttgagaaaaa tcaatgcctt caagttcctt
    93781 tgtgttaaca cctggaatca tctctatcaa tttatttata ggtccaagct ttttcatttg
    93841 atccatagct gataagtagt catcaaagtt aaattcttga tttaacattt tttcacttaa
    93901 cttacttgct tcatcttggt caatagcttg ctgagctttt tctattaaag aaaggacatc
    93961 tcccattcct aatattcttg aagccattct atctggatgg aatacttcaa tatcactcat
    94021 cttttcacca acaccaacaa atttaattga cttgccagta atgtctctta ttgaaagagc
    94081 agcaccacct cttgtatcac catctaactt tgttaatatt attcctgaaa gatctaatga
    94141 gttattaaat gtctctgcta cattaacagc atcttgacct gtcatagcat caactactaa
    94201 taatatttca ctaggatttg aaacttcttt aacatctttt aactcttgca ttaaatcttc
    94261 atctatatga agtctaccag cggtatctat tataacaacg ttatttccat tgtcttttgc
    94321 atgctctatt gcggctttgg caatatctac agccttaacc ttatctccca ttgagaatac
    94381 tggtatatct atttgttttc ctacaacctg taattgcttt atggctgcag gtctatatat
    94441 atcacaagcc acaagtaatg gctttttgtt tttctttctt aagtgtaatg caagttttcc
    94501 agccatagtt gttttacctg caccttgaag acctactaac atgaacactg ttggtccatt
    94561 gtctgagtat tttaatttac tctcagtctc tcccataaga gttgttaatt cgtcattaac
    94621 tattttaata acctgttgac ctggagttaa actatttaat acttcctcac caacacattt
    94681 atctttaaca tttgatataa aattttttac caccttgaag ttaacatctg cctctaaaag
    94741 cgcaagcttt acttctctca tggcttcttt tatatctttt tctgttagct tacctttacc
    94801 ctttaatttt ttaagcgtgt cttgtaattt agacgctaat ccatcaaaag ccatgcttaa
    94861 ccctcctaag cgtttataat ttcctctaat ttctcatcta tatatttttt tatatcttca
    94921 tctaaattag agttcttatt taattctgcc ataagctctt ctttaattct atacctttta
    94981 tcaacttttt ttgataattt aagtttttca tcataagaat gaagttgctt agagcatctt
    95041 ttgattaaat cataaattgc ttgcctactg gttttattaa tttcagcaat ctctgctagt
    95101 gacaaatctt cattgtaata aagggtcata acattaaact gcttttccgt aagcaatgtt
    95161 ccatagtaat ctattaacat tgatatttca aatctatttt ccatataacc accttgctca
    95221 cgaataagat aataacaaat aattttataa gtgtcaagta tttttactta acacttattt
    95281 ttttgatatt tatgcttaaa aacctgatga ttacttatat aaaatactat ctattcattt
    95341 atgattaata atttttctaa atattttttt atttttttct tataataatg cttctgcaaa
    95401 ttccttagca ttgaattttt gtaaatcctc tacaccttct ccaacaccaa tatatttaac
    95461 tggtatattt aaagtatttt taatagatat aacaactcca cccttagctg ttccatctaa
    95521 cttagttaat attattccat caataggaca agcctccata aattgttttg cttgtataac
    95581 tgcattttgt cctgttgtac catctaatac tagaagagtt tctttttttc tatctcctaa
    95641 ttctctatct attattctat ttattttagc taactcatcc ataagatttt tcttgttatg
    95701 aagtcttcct gccgtatcac atattaataa atctacatcc ctagctttag cagcctgtac
    95761 ggcatcaaaa actactgctg caggatctga tccttcttga tgcttaacta tatctacatt
    95821 agctctttga ctccatatat ctaattgatc tatggcagct gctctaaagg tatctgcagc
    95881 ggctaataga acttttttac cattattttt atttctagca gctatcttac ctattgaagt
    95941 agtcttacct actccattaa cccctattat aagcataact ttcttttcat tatcctcttc
    96001 ttcctcttca gagtctcctt ctagcatcat ttctgcaata acttctttta aggctggttt
    96061 aaccatttca acatcattta ttttttcttt tcttatttta gcttttaatc tatcaattat
    96121 ttcaatagta gtatccattc ctatatctga cattataagt gcttcttcaa gctcctcata
    96181 catatcatcg tctatagtta cagctaaatt taaagcctca tttattttat ctgttaagtt
    96241 atctcttgtt tttgttaatc cagtttttaa tttatcaaat aattttccaa acattttttg
    96301 tcctcctcta tttgtccaag tctactgaaa caattttaga tatacccttc tcttccatag
    96361 taactccata cataacatca gcagcttcca tagtaccttt tctatgtgtt attactataa
    96421 actgagtatt atctctaaac ttacctagaa actctgcata ccttcttaca ttggcatcat
    96481 ctaaggcagc ctcaatttca tctaaaatac agaatggtgt aggtttcatc tttaatatgg
    96541 agaataataa ggctatagca gataaaacct tctctcctcc agacataaga ttaatatttt
    96601 gaagcttctt tcctggtggt tgaacattta tgtctatatt tcctgtaagt tcgtcaccct
    96661 cacctaaaac aagctttgca ctaccacctt taaataattc tttaaaggtc tcatcaaata
    96721 gcttatttaa aatattaaag ttttgtctaa agactgctct catcttagat gtcatttctt
    96781 ctataacatt taaaagttct tccttagctt tttctaaatc atctctttct gttgtcataa
    96841 aattatactt ctcagatatt tctttaaatt cttcaatagc atttacattt acatttccta
    96901 aaccagttat tctagcttta agagttgata tcctaacctt atgagaatcc atatcttcaa
    96961 taggcttaaa tctatttttc tcttcttcaa aacttgtatt aaattcttct agaagtcttt
    97021 tgtttaagat atttttttca ttttcttgtt ttgtataagt tatatcattt ttatgaactt
    97081 cttcttcctt tttagctatt tcaactgaaa gaacttctaa gatattctca gcagagctta
    97141 tgttctcttt taacttacct tttttaagtt caaaggattt aaagttctct tcaagagtaa
    97201 ttatatttac ttcctcttct tttatgaatt tctcacataa aactatttct tttttacttt
    97261 cttccttatt agtttttata tcttctaagt acttagtaat atttataagc ttattttcca
    97321 cttcttctat agaagaattt attctattag cttccttttc tcttccctca ataacttctt
    97381 gaagcttagc tttatttatt ttcatattag taagcttatc tttatattta gaaagagttt
    97441 ttgaaagttc ttcacgtttc ttatctaatt cctttacctt ttctgaatta gttacttctt
    97501 tgttttcata ttgactaatc ttttcattaa caatttcaat ttctttgact atcctaatct
    97561 ttcttgaatc aatatcctta agctctaaat ctaaagattt tatagaattc tttattttaa
    97621 aagtttcatc attaacattt ttaatcttgt tatttaagtc cgctaattct aaattctttt
    97681 catatatttt atctataatt tcttgttttt ttatgcttaa ctcttcttga tcactattta
    97741 acttttccaa ttctttagtt agtatgtgct cttcattttt tactctaact aattcatcct
    97801 ctaaagcttt aacttcattt tttctagaca ttatgcctgt gtttttttga taaagactac
    97861 cacctgtaag tgctccacct gcatttacaa cctcaccaga taaggtaaca atcttatgtc
    97921 tgtaattaag cattttagca atttttatag cattatctat attatcacat attatagttt
    97981 tacctaatac ataatttata acattattat aaacctcttt aaagtttact aaatctgaag
    98041 ctattcctaa gaatccttca ctttcaacta tatttttgct aagttctaac ttcttacctc
    98101 ttattattga taatggtaag aatgtagctc ttcctagctt attttctcta agatgattaa
    98161 taagaacttt tgcaacttcc tcatcttcag ttataatatt agaaatactt ccacctaagg
    98221 ctacttcaat agaagtttca tatccagcct tagtttccac aacctctcct aaaactttgc
    98281 atttgttttt atttatttta acacgtccag catctacact agccattatt ttctttactg
    98341 aaaggttgta tccttcatat tgattttcta atttttttag catttcttta ttagcttcaa
    98401 tcttatttac ctttgataaa atttgattaa gtttaccttg agtttcctta attattttta
    98461 aaccttctct aaactcttct tctttttccc ttaattctct ctctttatct ttcttttctt
    98521 cttctataat ttgaagctct ttttgaagat ttaaattagt tgctaaatta atttttaatg
    98581 aattattata atttacttgt ttgctttttg atgaatcaag tttttcattt atgttactca
    98641 attcactttc taacattgtc attttatttt tagataaaga aa
//
LOCUS       DAEVGI010000002        75379 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEVGI010000002 DAEVGI010000000
VERSION     DAEVGI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 75379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75379
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.002"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..657)
                     /locus_tag="K8M77_000093"
     CDS             complement(<1..657)
                     /locus_tag="K8M77_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084625.1"
                     /translation="MAHNKSYRTFIILQEDEKGHSMASDKPLTGYAKIETKNDKCKVS
                     FYAQNLKKEYKDCYMMLICNKKDFKKNINLGPMNINQQGKAEMSLEYDSINIGDLNVS
                     YENIVGAAIGKNINGRTVFFMCGFLNNQMPKDNWKNYEIKMISGKEKSHYDKNNKEDK
                     MKRECPINMKEDKEKYNDDKDYYKDKKDKDEKDKYKEYLKEKDKDYLKEENKEKECKK
                     E"
     gene            complement(757..1614)
                     /gene="rsmA"
                     /locus_tag="K8M77_000094"
     CDS             complement(757..1614)
                     /gene="rsmA"
                     /locus_tag="K8M77_000094"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBI7084626.1"
                     /translation="MDINEIKDIKTKELVQKYNFRFSKSLGQNFLIDDSVPRDIVNGA
                     DVCEDDLVIEIGPGVGTLTVQLLKRAKRVVAIELDSSLIPILTAELGDNPKFQLIHND
                     ALKVDFNEIIGDEKSVKLVANLPYYVTTPIIVNLLKGGYNFKSLTIMIQKEVAERMNA
                     EPNCKDYGALSILVQYYCNTKIVRKVPPSCFIPRPKVDSIVIRLERLEEPSVKVKNEK
                     LFFEIVRHAFNMRRKTLWNATKNVKLPKELMEKAYEEAGIDPKRRGETLSLAEFGALS
                     DAIDKYMNN"
     gene            complement(1626..2174)
                     /gene="rnmV"
                     /locus_tag="K8M77_000095"
     CDS             complement(1626..2174)
                     /gene="rnmV"
                     /locus_tag="K8M77_000095"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBI7084627.1"
                     /translation="MIKEVIVVEGRDDITAVKKAIDAEIIAVGGFGINAKVIERIKEA
                     QKRKGVIVFTDPDFAGEKIRSIISKRVKGVKHAYIGRAEGTRAKDGNIGVENASPEAI
                     IRALENAKITLEEKREEFTIQDLIYFGLSADPKAKVRRELLGKELRIGYGNANQVLSR
                     LNNYGITKEEFVKAIEKIEKMI"
     gene            complement(2315..3337)
                     /locus_tag="K8M77_000096"
     CDS             complement(2315..3337)
                     /locus_tag="K8M77_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF348 domain-containing protein"
                     /protein_id="HBI7084628.1"
                     /translation="MKKIIKALKEKVASKNGKIVLGVFSLVLVSFIVVLVTAHKNITI
                     NLDGENLDVATFKGNVQAVLSQQGIYLSDKDKIEPSLTSEIADNDVISIKKAVPVEVM
                     VDGKNIEIDSAEDSVKNMLFKEGINLNDLDKVEPSLETELSKDLKIKITRVEEKTIVE
                     KEPIDFETVVKEDNSLESGVKKTTQEGASGEKEITMKLVMENGKEVSREVVESKVVKE
                     PVDEVVVKGTMEKLVLSRGKDNINYKKSMVVEATAYSGDGITATGTVPKRDPNGLSTI
                     AVDPRVIPLGTKVYVEGYGYAVAEDTGGAIKNNIIDLFLNSAPECKQWGRRNVNLYII
                     AYPGEW"
     gene            complement(3580..4350)
                     /locus_tag="K8M77_000097"
     CDS             complement(3580..4350)
                     /locus_tag="K8M77_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBI7084629.1"
                     /translation="MKYKIFDTHAHYDSDSFDEDRENVIKELKENGVIGVLNCGSDLY
                     GLRKSVELAKEFDMFYAAVGIHPENADEFNEDVVKEIKEFVKNEKVKAIGEIGLDYYW
                     EENPPREVQKEVFRAQMKLADELNLPVVIHDRDAHKDTLEIMKEFPNVIGVVHCFSGS
                     VEFAKECIKLGYYIGFTGVLTFKNAKKLVDVCREIPAERMLVETDCPFMAPVPFRGKR
                     NKSDYIEYIIDKMSEIRGISGEEMNEILLNNKKRLFKI"
     gene            complement(4369..5868)
                     /locus_tag="K8M77_000098"
     CDS             complement(4369..5868)
                     /locus_tag="K8M77_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBI7084630.1"
                     /translation="MKIYIIGALIGAVIGYITNWLAIKMLFRPREAKYIFGMKLPFTP
                     GLIPKEKSRIANKVGETVGTHLLNSDSLSKALKDDKIKAKFNEVAKEKINQVINSNST
                     LEESLKNTLGENYYALKGNMINNITKTILESIQEEEFKNKVKFYIVDSIKERLNKEPE
                     KIIDFIKSNKFREVIINTLGEEKTRDIIGKALLKEVKTLGKEDLTIEEVIPENIKPYI
                     EEYVKSQKDTLVDIIKNLLRDNEVSHKIKSAINDNIPSIVSMFLSGDVIYGKLVSLVD
                     KSLSEEENKEYICDAALAFVHESMKKKVSDVINTVGEEKLEVISDALGDKISKKINTE
                     ENIDSIIRKLNCKISSFNSYEEIIKVLFNDYENILIDNIDSMISQIVNNNQLASGISK
                     IIEKLFDKFLQNSLNDICYNKQNLENSIMSILDNLYNDFVENKSAKVLEIVDISSIVE
                     EQINAFEVDYAEEIIIGIANKELKAITWLGALLGGILGILSPLLSTIYM"
     gene            5964..6644
                     /locus_tag="K8M77_000099"
     CDS             5964..6644
                     /locus_tag="K8M77_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBI7084631.1"
                     /translation="MDLKKLSNIKECRLFSHFSDQDIQELLSSGFASVKSYSKEDLLF
                     SEGEYCNFLSVVIEGKIEIQKLDSNGNILVVATLNRGNVFGENLLFGDKNFYPMSVVA
                     KENSQILHIKKDYIYKLCSLNEAFLRELLRILSNKALNLSSKLKQVTMKSIREMICNY
                     LLKEYNKEKSTTIKLEFSRKDWADKLGVQRPSLSRELMKMRDAGLIEFSKNIIEIKDL
                     EGLKGYLL"
     gene            complement(6907..8451)
                     /gene="metG"
                     /locus_tag="K8M77_000100"
     CDS             complement(6907..8451)
                     /gene="metG"
                     /locus_tag="K8M77_000100"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI7084632.1"
                     /translation="MKVIIGNAWPYANGSLHIGRVSSWMAGDILARYHRAKGDEVIFV
                     SGSDCHGSPILNKAKEINKSPEELINKYHREFVRCFNKLGFSFDIFTRTDSEYHEKQV
                     KNIIKSLYDKGFIYEKETEEFYCQKCCDTLDEFGVKDGLCNECNSKVEVRSSNNLFFK
                     LSYFQDYIQNILDEEESWRENAIKITKRYLDGGLRDKILTREINWGIEVPVEGFEDKR
                     VYVWIDALLAYVTASKKVIEEKGQSLEEYWNNEDSRIYLVHGKENIPFHTTMFPAMLS
                     GIGLEKSEIRIFSSQYLTLEGKTFSTNRNWAIWVPYIMERYNIDSIRYYLISRGAEEK
                     NSDFTWRDFINANNNELVGELGNFTNRVLTFIKKNFNGEIKREELPLQWKNIIIRTYA
                     SVGNKFENGEFKSGVKEIFNLIKQGNDFFDKNKPWILINNNKKKCEEVLYICAQILVA
                     LTDLMNPIIPFTCEKIKMFLGIKDTKWSFEERKNIRVNRVEFLFDKIDKKAYIEELNI
                     LKNKKI"
     gene            complement(8552..10489)
                     /gene="metG"
                     /locus_tag="K8M77_000101"
     CDS             complement(8552..10489)
                     /gene="metG"
                     /locus_tag="K8M77_000101"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI7084633.1"
                     /translation="MCKKPYYITTPIYYPSTNLHIGNTYTTVAADAIARFKRLTGHEV
                     MFLTGTDEHGQKIERIANEKGITPKEHVDEIVAGIKDLWKMMNISYDKFIRTTDDYHV
                     KAVQEIFKKLYDQGDIYKDSYEGLYCTPCESFWTETQLVNGNCPDCGRPVEKAKEEAY
                     FFKMSKYADRLIQYIEEHPDFIQPESRKNEMLNNFLRPGLQDLCVSRTSFTWGIPVSF
                     DEKHVIYVWIDALSNYITALGYGQENQELYKKFWPADVHLIGKDILRFHTIYWPIMLM
                     ALGLELPKQVFGHGWLLVDGGKMSKSKGNVVDPVVLVNMFGADAVRYYLLREIPFGSD
                     GLFNNEIFIKKVNTDLANDLGNLLSRTIAMVYKYFDGVIQAPTCKEAIDDELINLALS
                     TPGKVEASIDALKIPEALESIWTLISRANKYIDETTPWILAKDEEKKERLGTVLYNLL
                     ETLRFVSVMISPFLTETSVKINDQLNTKVTTWESLKEFNGTVAGDKVVKGDVIFPRID
                     VEEKLAELEALKPAPVKPANEELVKNPIKEEITIDDFDKIDLRVVKVLECEPVKKAKK
                     LLKLKVDLGGEERQVISGIAQYYKPEELVGKYVVLVANLKPVKLRGELSQGMILAAAP
                     SDDSELVLVNPGEMLTGSQVR"
     misc_binding    complement(10567..10821)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10979..11560
                     /locus_tag="K8M77_000102"
     CDS             10979..11560
                     /locus_tag="K8M77_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7084634.1"
                     /translation="MKGLALPKIGARNLKTALAVTLCIGFFELIHRPYPFYACIAAVI
                     CMKDTVENSYKMGKSRMIGTITGGLVGLALTFIALNFNLTRFSGIITGIGIVITIYLL
                     NVFNRKGSVSIACIVLIAIMTNLHGKAPYAYAVDRIIDTFIGIIIALLINNYIYKYEN
                     KVKKDLENIEEKISRLEKEVKDDIKNLEENKKN"
     gene            11595..12104
                     /locus_tag="K8M77_000103"
     CDS             11595..12104
                     /locus_tag="K8M77_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7084635.1"
                     /translation="MFKFNLQTIGARNLKTSLSVFICIIVFELLGRSSAFFACIAAMM
                     CMQDTVENSYKMGKNRMLGTFLGAIVGLLLTAIFNVLSIQHFIVYALLTAIGTTIAIF
                     LCILFNCKGAINSACIVVFAIMTNLKGVGSYDYAFNRILDTFIGVIIAIIINRLVYPY
                     QKKTEEKTN"
     gene            complement(12144..13859)
                     /locus_tag="K8M77_000104"
     CDS             complement(12144..13859)
                     /locus_tag="K8M77_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBI7084636.1"
                     /translation="MRNKTRFKVFLTVFAMTMLCSTLVFAAEDAAAVNSAKFGMWTVL
                     PPLVSIVLAFLTKNVVVSLFIGTITGCIMLQLNGFNIITALTQGFIDFTYRALNSLAD
                     PWNAGIILQVLVIGGVIHLVAKMGGAKAIAEALARKAKTVKSAQLTTWLLGLAVFFDD
                     YANSLIVGPIMRPVTDKLGVSREKLAFIIDATAAPIAGLAIISTWIGLEVGLIGDALK
                     SVGIQADGFGVFLETIPYRFYNILILAFVVISAITLREFGPMLKAERRARRGEVLSTT
                     EISSDSEDLEPIEGIKLSVWNAIIPIGVLIVSSLIGFYYSGWVTIMGGEDLAIIELMN
                     NTPFSFAGIQAAFSNADASVVLFQSALLATIVALILGVSRKIFTISEGIGIWIEGMKG
                     LLITGVILILAWSLSSVIKELGTASYLVGVLSDAIPAFLLPAIIFILGSIIAFATGTA
                     YGTMGILMPLAIPLAFAINPEWNFVIVSTSAVLTGAIFGDHCSPISDTTILSSTGAGC
                     DHIEHVRTQIWYAVFVGIVTVLFGYIPAGLGLSIFIVLPLSLVALFILTMIFGKKVDD
                     KVVSN"
     gene            complement(14064..14201)
                     /locus_tag="K8M77_000105"
     CDS             complement(14064..14201)
                     /locus_tag="K8M77_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084637.1"
                     /translation="MNNLKRGRGARIKITKMELSSVEVLGKGLSPKFLANTLRLNAGV
                     V"
     gene            complement(14282..15895)
                     /locus_tag="K8M77_000106"
     CDS             complement(14282..15895)
                     /locus_tag="K8M77_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBI7084638.1"
                     /translation="MSKGQSAKRLKEIVRVFTYYGFDFLISSKLPNSKKAEPRPQALR
                     EALEELGATFVKIGQILSTRPDLLPKAYIEELEKLQDNNEITDFHKVKEIFYESFGTD
                     INTYFLEFSETPLASASIAQVHRAKLIDGRDVVVKVQHYKIDEKMKLDLSILRRLSKL
                     TSNHIANTLINPVEAFKEVEDATLKELDFEKEAKNTKIFRELNKNVACVGAPIIIDKL
                     TSKKVLTMEYIDGCKVTDFNILKEEGYDFEDIANKLANSFFKQVLEDGFFHGDPHPGN
                     LFIREGKIYFIDFGLVGTLEANLKNWLNKAMIAMVLGDIDTLVDFVNAIGIKKGKVGY
                     SVLYDDLKNIVSKYINASLKTIKISDLFKEIFEIAERNDIQFPRELVALVRSIVILEG
                     VIAKIDPDLEIMECIYPYVKERNKEEMLKSLDKDKLITEAYKFASKSVEIPTKFSELV
                     DSLTKGRAKLQFEMKGLDKPLTDLNRMVNRIAFSLIVGCMIIGSSLIVNAKTGPTFQG
                     VPILGLIGFIVSGIFGLWLLISIIKSGFF"
     gene            complement(16027..16365)
                     /locus_tag="K8M77_000107"
     CDS             complement(16027..16365)
                     /locus_tag="K8M77_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084639.1"
                     /translation="MINELKKAVLAGIGTAATAYEKTDSFIQDMVDKGKITVEDGKVL
                     SEELKRDMQEKTTEATSQIINKLDNMKPFTKEDFRVMFEEANKSTLEEINKLKERIAV
                     LEAKLNEEEF"
     gene            complement(16473..17717)
                     /locus_tag="K8M77_000108"
     CDS             complement(16473..17717)
                     /locus_tag="K8M77_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBI7084640.1"
                     /translation="MKSLVFTKNRENLLKKLEDNSLLVLFAGEAKRKTADEYFPFTPN
                     RNFYYLTGVDEEKHILMIKKINGVVDEVLYILKPNLEQERWTGKTIRDYEAKEVSGIE
                     NIKYLEEFKSDLNMIFTNGIAENLYLDLERVSFDEEMSKSQSFAKEIKERYPQVVIKD
                     VYSDIASLRQIKCKEEVEEIKKAAHITAKGVELLMKECKPGMKEYELEAYFDFYLKQN
                     GVKDYAFKTIAAAGVNAATLHYVDNNSEIKDGDLILFDLGAQVNYYNGDISRTFPANG
                     KFTKRQKEVYEEVLKVNEEIINSIRPGVGFYEINDKANNLLAEACVRLGLIEDKKDYR
                     KYYFHSIGHSLGLDTHDVGKRDIILEEGMVYTVEPGLYIEEEAIGIRIEDDVLVTKDG
                     CEVLTKECIKSVEDIEKFMSNR"
     gene            complement(17865..20462)
                     /gene="adhE"
                     /locus_tag="K8M77_000109"
                     /gene_synonym="adhC"
     CDS             complement(17865..20462)
                     /gene="adhE"
                     /locus_tag="K8M77_000109"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBI7084641.1"
                     /translation="MKVTNVDELMLRLEKVRAAQKEFSKFSQEQVDEIFRQAAVAANN
                     ERIRLAKMAVEESGMGIVEDKVIKNHFAAEYIYNKYKNEKTCGVIEKDESFGITKIAE
                     PIGVVAAIVPTTNPTSTAIFKALISLKTRNAVFFSPHPRAKNSTIEAARVVLEAAVKA
                     GAPKDIIGWIDEPSVEMSQVLMAEADIILATGGPGMVKAAYSSGKPAIGVGAGNTPAI
                     IDETAHLKMAVNSILLSKTFDNGVICASEQTVLVVDKVYDEVRKEFADRGAYFLKDKE
                     IDKVRKTILINGNLNANIVGQSAWKIADMAGVKVPKDTKVLIGEVESVELEEPFSHEK
                     LSPVLGMYRVANFEEALVKAERLVELGGFGHTSVLYTDTMKSQDRVKKFGATMKTGRT
                     IINMPSTQGAIGDIYNFKLAPSLTLGCGSWGGNSVSENVGPKHLINVKSVAERRENML
                     WFRVPEKVYFKYGALGVALRELKDLNKKKVFIVTDNVLASLGYVDKITNVLEEIGVDF
                     RVFSEVTPDPTLECAKKGAEAMRSYQPDTIIALGGGSPMDAAKIMWVMYEHPEVAFED
                     LAMRFMDIRKRVYPFPTMGQKAMMISVPTSAGTGSEVTPFAVITDEESGVKYPLADYE
                     LTPDMAIIDAELMMNMPKGLTAASGIDALTHALEAYVSVLASEYTNGLALEAIRLIFK
                     YLPQAYAEGTTNIKAREKMAHASTMAGMAFANAFLGVCHSMAHKLGSEHHIAHGVANG
                     ILIDHVIRFNAVDNPRKQAAFPQYKYPNAKWRYTVIADYLGLGGKNEEEKVNNLIKAI
                     DNLKKTVGIPATIADCGVSKEAFYATLEKMSEDAFDDQCTGANPRYPLISEIKQMYIN
                     AFEGKKDRV"
     gene            complement(20888..21406)
                     /locus_tag="K8M77_000110"
     CDS             complement(20888..21406)
                     /locus_tag="K8M77_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBI7084642.1"
                     /translation="MIANNIIPIIFLLIIVYGMFKGRKVYEWFLEGAKEGLMVCVKIF
                     PALLAMMIAIRIFKDANLLNYLNNLLEPIVSLIGLPKDIVPLVLIKPLSGSGALGVYT
                     ELINTLGPDTKEGLISSVIMGGTETIFYTITVYFGAVGIKKIRHTFWAALMADITAII
                     MAVLVVSMFFGL"
     gene            complement(21418..21996)
                     /locus_tag="K8M77_000111"
     CDS             complement(21418..21996)
                     /locus_tag="K8M77_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBI7084643.1"
                     /translation="MINYIWFAIIALGLIFSIMTGQGELITTGLTESSEGAVKFIISL
                     VGIMCFWCGVMNIAENSGLTSKVAKLLNPILKKIFKESSHSDEAMGAIVMNLTANMFG
                     LSNAATPLGIKAMSELNKINKEKDGRASNDMALFLVINAACIQLIPSTVISIRAAAGS
                     SEPASIIIPAIICTFTACCVGIICCKILQRYF"
     gene            22111..22290
                     /locus_tag="K8M77_000112"
     CDS             22111..22290
                     /locus_tag="K8M77_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084644.1"
                     /translation="MNEKNLKLAQQDIDEVLKTVEELEVQIDDLSKNDLKQKFVTLTE
                     KVRKLEDLLKDEGII"
     gene            complement(22330..23697)
                     /locus_tag="K8M77_000113"
     CDS             complement(22330..23697)
                     /locus_tag="K8M77_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7084645.1"
                     /translation="MKDQKLLGEESELKLLIKYSVPAIIGMLVNALYNIVDRIFIGHI
                     PGVGPMAITGVGITMPIMSILLGFGMLIGIGATANISIKFGQNKRKDAEKILGNSVVL
                     ITIISLVLTIVGIVFANSILHLFGASEATIFYAKEYINVILLGTIFNLMSFSLYSTIR
                     ADGNPKMSAAVMVLGCIINVILDAVFIFVFNLGIKGAALATVISQIVTTLIMLYYYTF
                     GGSNLKLKFETLKLDWRLVKIVLAIGIAPFSMQMAASVVQVIANNALRMYGGDLAIGA
                     MAAISSIAMIFLMPIFGINQGSQPIIGYNYGAKKYERAQKTVKLAMIAATTILVLGGI
                     LIQAFPALVISMFNSDPKLLEIGVPGLRIYLFMMPIIGISIIGSNYFQSIGKAKLATF
                     LSLLRQVILLIPLTLVLPKIAGLGLTGVWLAGTVSDFLSTIITGLFIIKEFKKEDSIE
                     NEKEI"
     gene            complement(23886..24518)
                     /locus_tag="K8M77_000114"
     CDS             complement(23886..24518)
                     /locus_tag="K8M77_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084646.1"
                     /translation="MDQNINKKLSVGQFLKSVFNIFIKNLGDIAIISVLFALPTIIGR
                     GNAIFSVIGIFSLGFSSIAIIKLANNFIRGEKVSWIETIKSSFKNSLFPLGVFLIQNF
                     AVSLGSSIFAPLGIVISIFFVIAMQCSIFENINVIESIKKSFLLVKNNFLDILLKQFA
                     LVFIINFFTMTFAMFLNQSVLSIIIFSLVLNIITALTLIGGNLIYKEVTV"
     gene            complement(24631..25323)
                     /locus_tag="K8M77_000115"
     CDS             complement(24631..25323)
                     /locus_tag="K8M77_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HBI7084647.1"
                     /translation="MEFLAYFNSVISNNTFLAIILSFFAGVVASFSPCTLSSIPLLIG
                     YVQGSEIKDNKKAFKYSLIFSIGLGITFTVIGLLTALIGKAFLGAGKFWYIILAFIMI
                     GSGLQVLDVINLFGDKKDACKITKRREGILDVFFLGILSGILASPCATPIMAAIIVFI
                     AASGNLVIGMIMLLMYSLGHSVLIILAGTSFGLVEKIAYSEKGKKTGRVLKNILGTII
                     ILVGLYLFYLGV"
     gene            complement(25327..25755)
                     /locus_tag="K8M77_000116"
     CDS             complement(25327..25755)
                     /locus_tag="K8M77_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBI7084648.1"
                     /translation="MKNKRKLIILGLILFSLICIYIGKNYIEGIKQINFQSINVVENL
                     EEAKEGIPTIIMFKTDTCPYCVEMQKELSYVSKEREGKFNIYYARLEEEKNIDLAYKY
                     DANIVPTTVFLDKEGNKFYVHQGLMRKNNIETILNSLGVK"
     gene            complement(25860..26858)
                     /locus_tag="K8M77_000117"
     CDS             complement(25860..26858)
                     /locus_tag="K8M77_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7084649.1"
                     /translation="MAASIKDVAREAGVSIATVSRVLNDIDVVNEETKKKVLEAIKKL
                     GYRPNIVARSLKTHRTRTVGILVPDISNQIYPEIVRGAEDVANIYKYNVMLCNSDLDL
                     EKEKEYLRVLKEKMVDGVLYMSSSLGEETLNLINELDLKTVLVENDEQNGKFPSVTID
                     NEKATYESTNHLIDKGMKNIAFIGVEGDLNNAWGKRYKGYQAALKDAGIEMKEELTYF
                     KNLKVKTGYDAILAMLENGQKIEGIICASDEIAMGAINALRDKGIKVPEKVSVIGFND
                     IDLAHVFYPKLTTVSQPMYDMGSIAMRMLIKIINNKPLDQDHFVLDHDLIERDSCK"
     gene            27189..27518
                     /locus_tag="K8M77_000118"
     CDS             27189..27518
                     /locus_tag="K8M77_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBI7084650.1"
                     /translation="MYKDIDITESIKLIDENKDLLILDVRTEEEYKEDGYIKNAKLIP
                     LGRLLFSIDELDGYEDSPILVYCRRGSRSMQACEILEDNGFTELYNMMGGFSKLVNEG
                     PKELWNK"
     gene            complement(27575..30694)
                     /locus_tag="K8M77_000119"
     CDS             complement(27575..30694)
                     /locus_tag="K8M77_000119"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBI7084651.1"
                     /translation="MYKKVELPKGFVGVEKEVANLWKEKNIIKKNFDMNQDGEYFTFY
                     DGPPTANGKPHVGHVLTRVMKDIIPRYKVMKGYKVIRKAGWDTHGLPVELEIEKKLGI
                     SGKPQIEDYGVEKFVTECKDSVFTYVSMWEKMTEQIGYWVDMENPYVTYHNPYIESVW
                     WALKQMWDKKLLYKGYKTMPYCPRCGTSLSSHEVSQGYKDVKDLTAVAKFKVKGEDNK
                     YFLAWTTTPWTLPSNVALCINKAYDYVEAKQAEGDEIFILAKELAPKVLGEGFEIVRE
                     FKGEELLGMEYEQLLPFVTPTEGKAFVVVHGDYVTLSDGTGIVHIAPAYGEDDNLIGK
                     QNGLAFLNLVDLSGNFVEEVTPWAGKFVKKCDQQIVDYLKEQNKLFKAEKHTHSYPHC
                     WRCDTPLLYYPKESWFVAMTTLRDKLLANNEKINWYPDNIKNGRFGKFLENVIDWGIS
                     RDRYWGTPLPIWECECGHRECIGSIEELKTKGINVPEDIELHKPYIDKVHLNCPHCNK
                     EMKRTAEVIDCWFDSGSMPFAQHHYPFENKELFEANYPAQFISEAVDQTRGWFYTLLA
                     ISTAIFDRNPFENCIVLGHVLDKKGLKMSKSKGNVVDPFEVLDSQGADATRWHFYTAS
                     APWLPTRFSIEDVAETQRKFLSTLWNVYSFYVLYAELDNFNPLDYKDFVSDNVMDKWI
                     MSKLNTLVKDVDDHLNNYRITQAALEIEEFTDELSNWYVRRNRSRYWSEELTDDKIGA
                     YTTLYRVLVTLCKVAAPFVPFITEEIYQNLVVNLDENALESIHLGAWPEVDEKAIDKK
                     LEEKMDLAYKIVKLGRSARNGANIKNRQPLSKMLLSTSELPDYYGDIIKDELNIKEVE
                     LGADLSKYVNFEIKPNLPVLGRAYGKLIPAIRKEIASRNQMELAQKIQNGGVEVITVD
                     GNEIELNAENLLVTMQGLEGFAFAGEGSVGVVLDTTITDELREEGHVREIISKIQNMR
                     KESGFEVADKITLYVAENDMLLDVIKKFEDVIKKETLTEEIVYNGDADYSEAKVNGEI
                     LKMAVSKRA"
     misc_binding    complement(30795..31076)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            31361..31576
                     /locus_tag="K8M77_000120"
     CDS             31361..31576
                     /locus_tag="K8M77_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBI7084652.1"
                     /translation="MNDNNNLNQQVIDKMTKVCICRAISRAKIKEAIKNGAHTVEAVA
                     EKTGATKGSCKGCRCRDKIEELIEDNQ"
     gene            complement(31743..32351)
                     /locus_tag="K8M77_000121"
     CDS             complement(31743..32351)
                     /locus_tag="K8M77_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBI7084653.1"
                     /translation="MQKYCKNQNNINILLDHESGRGFKVTFTINEEGESNILVITRAP
                     KSHNAEDLKKELNKAIKYLMNNEDELGIIKEISFVFLFPVIEYSKDVLEEVYNTKGEL
                     FLCGNDGFIENGEYLKNDLIIFESLINSSHIIFAWGEPTSKDIGKVYESRLHYLLKGF
                     KVVKNNCFDLKKFYYVGTLTNQGYPRNYSSWSKNLQLNEFEI"
     gene            complement(32515..34443)
                     /locus_tag="K8M77_000122"
     CDS             complement(32515..34443)
                     /locus_tag="K8M77_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBI7084654.1"
                     /translation="MNLITLENISKSYSEKILANNVSLGINEGEKIGLIGVNGTGKSS
                     FLKIVAGVEEPDEGTVTKGNRVRIEYLAQTPDYDDNATVLEQVFKGNSEEMRILREYE
                     ELLEKIDKGEVKENDSERLIKLQGKIDALNLWDMESEAKNVLTKLGITNFEEKVGNLS
                     GGQKKRIMLAAALITPCELLILDEPTNHLDNETISWLEEYLNSRKGALLMITHDRYFL
                     DRVTNRILELDRGRLFSYDGNYSVFLEKKMERIAIEKASEEKRQNLMRKELAWVRRGA
                     KARTTKQKARLQRFDELVNQEGFIENENIEISVMGTRLGKKIIEIEHLNKRFGDKKIV
                     DDFNYIVLKSDRIGIVGPNGIGKSTLMGMIEGKVKPDSGEIIKGETVKIACFSQEDTH
                     MHPEMRAIDYIKEAGEYLQTATGERITASQMCEKFLFDGTLQWTMIGMLSGGERRRLH
                     LLRVLMEAPNVLLLDEPTNDLDIETLNRLEDYLDDFGGVVITVSHDRYFLDRVCNKIF
                     SYEGNGKIDIYTGNYGDYLLSKEEEAVNNKEKEVAVDKNKKESSVDKNKNKGKVLKFS
                     FKEQREFETIDEEIMTLEEKIEELDSLMAKHASEYGRLQELIEEKANVEEELAFKYER
                     WEYLNELAAQIEENKKNK"
     gene            complement(34650..34964)
                     /locus_tag="K8M77_000123"
     CDS             complement(34650..34964)
                     /locus_tag="K8M77_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084655.1"
                     /translation="MIKAIVIPTPKRLKKYIRDTDFKKKAKSFVTPERAIIYGTIGTG
                     VTIMSLSDVPAKPFVIGSLLYHGIAYEILEKWYDEYGNDEELREMMMQNRDYWNNFYM
                     RF"
     gene            35124..36299
                     /locus_tag="K8M77_000124"
     CDS             35124..36299
                     /locus_tag="K8M77_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBI7084656.1"
                     /translation="MASKFYLHKGKNKKAEQGRPWIYIDEINEYDGDYENGDIVEVYN
                     HKGYFLGKGYINDRSKITIRIMTKDIDEEIDEDFFKRRFKTAWEYRKKVIDTSSCRFI
                     FGEADFLPGLTVDKFEDYYVIQISTLGMDKYRDLIVKILVEEYGAKGVYERSDIKTRE
                     IEGLEQRKGFLTEPFDTDIQIVENGVKYIVDLENGQKTGFFLDQKENRAAMHRICKDM
                     DVLDCFTHTGSFALNAGIAGAKSVLGIDVSQHAVDCATRNAELNNLQDRVKFEKHNAF
                     DVLGDWSREGKQFDVVILDPPAFTKSRNTVKQAIRGYKEINLRGIKMVKEGGYFATCS
                     CSHYMDEEQLKKTVAEAAHDARRTLRQIEVRTQSADHPILWNSDESYYLKFFIFQVF"
     gene            complement(36664..37011)
                     /locus_tag="K8M77_000125"
     CDS             complement(36664..37011)
                     /locus_tag="K8M77_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HBI7084657.1"
                     /translation="MVKELICIVCPRGCHLTVDTELEKVTGNTCKRGEIYGLNEVKNP
                     TRIVTSTVKIDGCDNIVVPVKTEEPIPKGMIFDVMKEINKASVKLPIKVGDVIIEDVL
                     GTGVSIVSAKTVS"
     gene            complement(37014..38267)
                     /locus_tag="K8M77_000126"
     CDS             complement(37014..38267)
                     /locus_tag="K8M77_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7084658.1"
                     /translation="MKYDLVVVGGGPAGLAAAYEAHENGVEKILIIERDKELGGILNQ
                     CIHNGFGLHTFKEELTGPEYAGRFIDMVEGTNIEVMLDTMVLEIEGKIIHAINTEKGY
                     LTIEAEAIVLAMGCRERTRGAISIPGDRTSGIFTAGAAQRFINMEGYMVGKKVVILGS
                     GDIGLIMARRMTLEGAKVEAVVELMPYSNGLTRNIVQCLEDYGIPLYLSHTVVDVKGD
                     GRLEKVIIAKVDENRQPIKGTEIEFDADTLLLSVGLIPENELSKNAGVELDLRTNGLV
                     VSESMETNREGIFACGNVVHVHDLVDFVTEEAKNAGKNAAKYIKGKKRLNHFTEIKNG
                     ENISYTVPQKFEIESLDGNLNVFMRVRNVFKNKQIVVRDEEGNVIQSFKKPHVVPAEM
                     ERITILKDKLKNAKGAITISLEDGE"
     gene            complement(38280..39710)
                     /locus_tag="K8M77_000127"
     CDS             complement(38280..39710)
                     /locus_tag="K8M77_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI7084659.1"
                     /translation="MIYDVAIIGSGVIGGAIFRELTKYNLKVVELEKENDVAMGASKA
                     NSAIVHSGYDPEPGTLMAKYNVEGNKIFDKLCKDLSVPFKRNGSLIIGFDEDDQKELE
                     VLYNRGIQNGVEGVKLLSAEEVLEMEPNLNKDVKGALFCPTGGIVGAYEFTIALSENA
                     IDNGGELRLNSKVVGIEKDENFKIKLENGDVVEARFIVNAAGIYADKIHNMVCEEDFK
                     ITPRKGEYYVLDKSQGRLFEKTIFQCPTKLGKGVLVTPTVHGNLLVGPDAIDTCDKDS
                     VSTEYYGLAKVREVSLKTTNKVNFRENIRNFAGLRAIADKPDFIIGEAKDVKGFFDVA
                     GIKSPGLSSAPAIAVDVVKMLGESGLALEKKDNFNEKREQVHFIELSPEEKANLIKKD
                     SRYGRIICRCENITEGEIVDSINRTLGARTIDGVKRRCRPGCGRCQGGFCGPRVQEII
                     SRETGMNIEEVLLDKNGSYIISGKTK"
     gene            complement(39876..41378)
                     /gene="glpK"
                     /locus_tag="K8M77_000128"
     CDS             complement(39876..41378)
                     /gene="glpK"
                     /locus_tag="K8M77_000128"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBI7084660.1"
                     /translation="MKKYIVALDQGTTSSRAIIFDKEQNIIGVSQKEFNQIYPREGWV
                     EHDPMEIWATQYSVLQEVMAKCNITQENIAAIGITNQRETTIVWDKNTGVPIYNAIVW
                     QCRRTADICDDLKERDGLVDYIRENTGLVLDAYFSGTKIKWILDNVEGAREKAEKGEL
                     LFGTVDSWLVWKLTNGKVHVTDYTNASRTMIFNIKNLEWDERMLKELDIPRSMLPEVK
                     NSSEIYGYANLGAKGGIRVPIAGIAGDQQAALFGQAAFNKGDVKNTYGTGCFLLMNTG
                     EELVKSKSGLLTTIAIGLHGKVQYALEGSVFVGGAVIQWLRDELRIISDSSDTEYFAT
                     KVEDNGGVYVVPAFVGLGAPYWDMYARGTIVGLTRGTNRNHIIRASLESIAYQTRDVL
                     EAMINDVGYDINCIKVDGGASRNNFLMQFQSDLVGKKVIKPIITETTALGAAYLAGLA
                     VGYWSDKEEIAKLWFASEEFEPNISEERRNKYYKKWKKAIERSKEWAIED"
     gene            complement(41398..41958)
                     /locus_tag="K8M77_000129"
     CDS             complement(41398..41958)
                     /locus_tag="K8M77_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBI7084661.1"
                     /translation="MINFKRILEDNPIVAAVKDMEQLNKALEADVDVIFVLFGNIMDI
                     IEISHKISEKNKIGILHIDLVDGLTNREIVLEYLKEKTKFDGIISTKAQTVKSAKKLG
                     LVAIQRVFILDSLSFQSTKNHLVDECDAVEMLPGLLFKVIKELSKTIHKPLIVGGLIS
                     DKEDVMEALKSGATCISTTKEDIWKI"
     gene            complement(42000..42707)
                     /locus_tag="K8M77_000130"
     CDS             complement(42000..42707)
                     /locus_tag="K8M77_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBI7084662.1"
                     /translation="MVNQFLAELFGTALLILLGDGVVANIALKKTKGNSADLIVVTVG
                     WAIAVAIPVYIFSRVSGAVFNPAVTIALGITGGLAWNQVVVFCIAQFIGAFIGAVLVY
                     LTYYTHFESTEDHDAKLGTFCTIPAIRNYKWNFFTEFIGTFVLMFGILGFGDAKMVDG
                     IMPIAVGLLILVIGVSLGGPTGYAINPARDFSPRLAHFLLPMKNKGGSDWAYAWIPIV
                     GPILGAIAGAIVYTNIF"
     gene            complement(43208..43951)
                     /locus_tag="K8M77_000131"
     CDS             complement(43208..43951)
                     /locus_tag="K8M77_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase family
                     protein"
                     /protein_id="HBI7084663.1"
                     /translation="MKIDVIISADYIDSEVLKGKIAVVIDMLRATSVINTALYNGAKK
                     VIPVVSVEEAFEKAKELKSLGEEVLLGGERKALKIDGFDFSNSPLEYKREIVEGKNVI
                     MSTTNGTRALNLCSKADKVIVASVLNGQAVAKYLENEEKEIVFVNSGTNGEFSSDDFM
                     CAGYIISELCKNKEVELTDIAKTAKYVYESSEGIEEFIKDAKHYNILKNLGLEEDLKY
                     CSTKNLIDLVFEFKNGEIKTVESGIKVKI"
     gene            complement(43978..44913)
                     /locus_tag="K8M77_000132"
     CDS             complement(43978..44913)
                     /locus_tag="K8M77_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450751.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBI7084664.1"
                     /translation="MEKRKTWGDKRYNTLNRFLREKFGEKVFKISLDGGFTCPNRDGK
                     VAKGGCTFCSSKGSGDYAGQRTLSIGTQFDNVKEMMNKKWKEGKYIAYFQAYTNTYAP
                     VEELRRKYEEAINKEGVVALAIGTRPDCINEEVLDLLEEMNKKVYTWVELGLQTIHDD
                     TARSFNRGYDLEVFDKAMEGLKSRGIDTVVHCIFGLPGESKEQMLETVDYVSRCGAQG
                     IKLHLLHLMEGTRMVKDYEEGRLKFLTQEEYVDLICTAISRIPQNMVIHRLTGDAPRQ
                     LLIGPMWSLKKWEVLNAIDKALEDNDIYQGKNFEE"
     gene            complement(45006..46091)
                     /locus_tag="K8M77_000133"
     CDS             complement(45006..46091)
                     /locus_tag="K8M77_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084665.1"
                     /translation="MVKKIMASLIIGSLFLFIGCNRISNKVNDNKELKESVESIYFNN
                     KIAISIAEEYMEAIKSNDLEKIKRLSDKEVDKNIIISPSKELEITGIKQVGAAQLGNK
                     TMFKFYVTRTKKGEPKGYLEQYYLEVRKTEDGEYKVSELKSTELYNVFLDKDKLKIRK
                     DDDVEINTLIDLKSIPDKIYPKVNVIDVAKVDVPKEAFSAVEISFSGDKVAISTYKGE
                     DSYIGVVDVEDAQETAAKEGEDEGGKEDEKNDDKTLGKKITSLDVLKGCKISSLNFSD
                     DDGYVVVNYVKGNGTRFKVYQSGGSIVSLELDDIFAEKKYNLIYERIQDDNIIMNVTP
                     IIESNNTESGLIGRYKISLKDFKLEKL"
     gene            46251..47303
                     /locus_tag="K8M77_000134"
     CDS             46251..47303
                     /locus_tag="K8M77_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HBI7084666.1"
                     /translation="MKSIIKVFQVAFVFIGTIVGAGLASGKEITNFFTVYGYKSFFTI
                     IMTGLFYIFLCNIISRISIKHSLNSYSEVINTVSPNILGKLTGIITTFYLISSASIIL
                     AGSGALLNQYFGIPNIIGSIFMAIIASLILMRETDGLVEINSIIVPSLIIVTTTLMIL
                     YVTFANDPITIGSLVSSTPKLSKGVFTSGILYCGYNILCCCGVIVPLSNEIKSKKIMT
                     WGIILGSVLLTIICIFINSMLMVNQPQIFQYEIPLLYIADRFGKPIQIMLLIIILSEM
                     FSTEVSDVYSISKTLTKIFKIKYKHAIFAVLIVALPISLIGFSNLISTLYPMFGVLSL
                     VFIAQCIYFYFFKLKK"
     gene            complement(47355..50558)
                     /locus_tag="K8M77_000135"
                     /pseudo
     CDS             complement(47355..50558)
                     /locus_tag="K8M77_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            complement(50713..51384)
                     /locus_tag="K8M77_000136"
     CDS             complement(50713..51384)
                     /locus_tag="K8M77_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7084667.1"
                     /translation="MSRVGERIKEAREKSGMTQKALAKKLGVAEKFVNEVETGRKIIN
                     ESLISKVSKVLNTDLNDINMVVTDEELQKELKAEKQLRQTKPADVNEVWNQAFGSVLK
                     NVPIYDYSLAQVKGYKQLATASNKIDGHTANKVFYLKIENNDMTGYRIQENDLALCYS
                     IKEIENNSICLVEFNGKRVIRQIKKLDNVKALLISNNGSMRTETANIKEVKAIAKLYR
                     IEFDL"
     gene            complement(51386..51760)
                     /locus_tag="K8M77_000137"
     CDS             complement(51386..51760)
                     /locus_tag="K8M77_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBI7084668.1"
                     /translation="MSMLNNSKAILTYGLNEKETKDFQATGHNIINISNEMASMKVKD
                     ILEGLKFEVVSKKNFDEKVVIFSNFPDEELQMMVSIAKVITENPIMAVVTETSKEWQF
                     NYLVEHLIEEREWYRSMQGGKA"
     gene            complement(51785..52297)
                     /locus_tag="K8M77_000138"
     CDS             complement(51785..52297)
                     /locus_tag="K8M77_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI7084669.1"
                     /translation="MNPIVTIEMATGEKIKIELYPEIAPNTVRNFVSLVKKGFYDGLT
                     FHRIIPGFMIQGGCPEGTGMGNPGYRIKGEFTRNQFPNNLKHSRGVISMARAMNFDSA
                     GSQFFIMHENSPHLDGQYASFGKVIEGMDVVDKIANVKTDYNDMPDEPQVMAKVTVEN
                     AEGIEEPEIL"
     gene            complement(52538..52807)
                     /locus_tag="K8M77_000139"
     CDS             complement(52538..52807)
                     /locus_tag="K8M77_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HBI7084670.1"
                     /translation="MKINVREFLGDKIHAEDAIILRDYIEDNLNENVELDFGGIGRVS
                     TTFLTCLFTDMINKEGRDNIISKINVKNLSNYTDYSRVVAGTTFA"
     gene            complement(53042..53824)
                     /locus_tag="K8M77_000140"
     CDS             complement(53042..53824)
                     /locus_tag="K8M77_000140"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI7084671.1"
                     /translation="MQDDLKNAPIGFFDSGLGGLSVLRKALEMMPNENYIYYGDSKHA
                     PYGEKTPQEIRSLSFNAIEFLIKKGAKAIVIACNTATSAAAHDLREYYKDIPIIGIEP
                     ALKPAIKLHETGSVIVMATKATLTQEKFKNLMDKYGEHREVIPLPCPGLVEFIEAGNL
                     EGEEVKNFLREKLNPYMDREISSIVLGCTHYPFVKDVIQDIVGEKVDIIDGSSGTIRE
                     LKRRLEENNMQSELKKKGDLDIFNSLEDNKILELSKKLIEIK"
     gene            54123..56024
                     /locus_tag="K8M77_000141"
     CDS             54123..56024
                     /locus_tag="K8M77_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBI7084672.1"
                     /translation="MNKDLLFTIPKKYHNKESLISIFEDHPEIKFVSLVGVDLAGNDT
                     DEKIPSKLFLKDIDSFLHGTAVQTDGSSVVLPGIATLNNAKVDMIADLDCKWFVDYNY
                     DFIDPLTNRPVGTLRIPCFLIHEDKAVDSRHILKNSIDTFKSNIFSLLKKYPETLNDL
                     NIKFDDIDDLLLTSATELEFWVKTPNDKALIEELSTSQVLQEQYWTRTKGNVRTALEQ
                     SLLYMDEYGFEPEMGHKEVGGVKAKLTSSGKFDHIMEQLEIDWKFSDAMQAADNELFI
                     RTLVKETFRRNGLEVTFLAKPIDGVAGNGEHTHLSLSLKLKDGRIINLFNPTKNHFLS
                     KIGYGSIMGILKNYEVMNPFISATTDSLKRLKPGFEAPVCIVTSLGQSPEIPSRNRTI
                     LAGLIRDPHNPLATRFELRSPNPFTNTYLCIASSYMAMLDGIKYALENNKTEDDLLAE
                     LSKKPGEEADYLETSRAYRSEEDVFEDFTDSQRNEYFGVAPATVFENLSAFDKYPEKV
                     EVLKVNSVFTDKLINSFKMATTKRWTTEITSRIIPSYTKDIRAAKQLHCCDKALDLDV
                     STWMTINELRHIIMKDSYHRRSLFTQIKNAINESDFEKASDLQIKLDKNMNELNDLYS
                     TYKKNLLDI"
     gene            complement(56113..56397)
                     /locus_tag="K8M77_000142"
     CDS             complement(56113..56397)
                     /locus_tag="K8M77_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084673.1"
                     /translation="MTKSSGMFVKINYKINGDNRFQGEHKAKMYKDTAVNKHVLCGGS
                     YFRNRGTIIFHANNFKEAEEIINNNPFVFYEDYSFEILSKNSISLGNTYH"
     gene            complement(56635..57276)
                     /locus_tag="K8M77_000143"
     CDS             complement(56635..57276)
                     /locus_tag="K8M77_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBI7084674.1"
                     /translation="METAKRVLKNFFIKSILLIISVFLAMAFYKLIVKECIDVFIKID
                     LSGKKGFVIYNFFKLTSVMLIYQVFLTTTRMKTSKLFKLFLFGLYAGTMFILLFARPK
                     MARGFQLDPFAVLHGLRGDRTGQLYLIGNIIFFMPIGYVLRKYNFVLAFILSASIEFN
                     IELAQYVFKRGFFDLGDIFINLVGIFIAYFICKIAYFIKYRLYKRDHEIEEVE"
     gene            complement(57891..61094)
                     /gene="carB"
                     /locus_tag="K8M77_000144"
     CDS             complement(57891..61094)
                     /gene="carB"
                     /locus_tag="K8M77_000144"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBI7084675.1"
                     /translation="MPLNKDIKKVLVIGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKVYLEPLTVEFVEKVIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYNVKVIGTSIESIKEGEDRELFRDMMNRINQPVIQSEIITDLDSGIAFAR
                     KIGYPVIVRPAYTLGGTGGGIANNEEELIETLTSGLQLSTIGQVLLEKSVKGWKEIEY
                     EVMRDSFGNCITVCNMENIDPVGIHTGDSIVVAPSQTLSDKEYQMLRSASIDIINAVG
                     IEGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVAAKIALGYGLDEI
                     KNAVTGMTYACFEPSLDYVVVKIPKWPFDKFQGADRVLGTKMMATGEIMAIGSNFEAA
                     FLKGIRSLEIGKYSLEHKKFKDLSMYELRERVVSPDDERIFALAEMLRRGYRIDMVSK
                     ITGIDIFFLEKFRWLVEEEQKLKQSTIDDLNREWLLKLKRRGFSDKAIADMLKVSPDE
                     IYRLRDIWHIKPSYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDNKKVVVIGSGPIR
                     IGQGIEFDYASVHCVMALRKQGIETIVINNNPETVSTDFSISDKLYFEPLTEEDVLNI
                     IDKEKPDGVILQFGGQTAIKLAKFLKEKNIPTLGTTSDQIDLAEDREQFDDLLERLNI
                     ARPKGKGVWSLEEGLEEARRLGFPILVRPSFVLGGQGMEITHDEEELTYYLTNAFEKD
                     SKNPILIDKYLMGREIEVDAISDGEDVLVPGIMEHLERAGVHSGDSITMYPAQNISDK
                     IKEDVLDYTKKLALSIGIKGMINIQFIEFEGKLYVIEVNPRASRTVPYISKVSGVPIV
                     DIATRIMLGEKLKDLGYGTGVYKEPELVSVKVPVFSTQKLPNVEVSLGPEMRSTGEVL
                     GVGRNVFEALYKGFVGASMYTGDKGKTILATIKKHDKKEFMELAKDLDKLGYNFIATT
                     GTANELREAGIDAKEVRRIGEESPNIMDLIKNKEIDLVVNTPTKANDSKRDGFHIRRA
                     AIERNIGVMTSLDTLKALVELQKEGAHNRELEVFNLI"
     gene            complement(61107..62156)
                     /gene="carA"
                     /locus_tag="K8M77_000145"
     CDS             complement(61107..62156)
                     /gene="carA"
                     /locus_tag="K8M77_000145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBI7084676.1"
                     /translation="MKAKLILENGVVFEGKAFGYLKECVGEVVFNTGMTGYQEVLTDP
                     SYYGQIVTMTYPLIGNYGINLEDLESKEPKVRGFIVREKCQYPNNFRCELELETYLAQ
                     NKVLGLDGIDTRALTKILRNNGTMKGIIVLDNSNLEDVKDKLEAFSNRDAVSIVSTNE
                     KYEISGEGKNVAIIDFGIKQNIIRNFVKRGCNVTVFPYDFKAEEVLDINPDLVFLSNG
                     PGDPEDMGEAVNEIKKIVGKKPIVGICLGHQLLALTLGGETKKLKFGHRGCNHPVKDL
                     INNRVHITSQNHGYYVATLPENMEITHVSMNDGTVEGMKHKELPIFSVQFHPEACPGP
                     KDSEYIFDEFMKYAL"
     gene            62814..63470
                     /locus_tag="K8M77_000146"
     CDS             62814..63470
                     /locus_tag="K8M77_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084677.1"
                     /translation="MEIIYPNDFVRFFNEDEEIDEIGEFEVLPEDTENSFERYYTGND
                     DTFNYNYPMNYRNSDNDSKFSVIPPVIKNIPDNIANVPAVIQDLPYAIKDMGNDLMNS
                     APVGYISNMLPIPTNWQSPQSPSPMGPPPSYIPSKKDTGVQNFGKESGNLNAKNVSSS
                     SIRFCLYRFTYIWQTNGRSYWAYLTRVDKKSVSGWRWAGFRWVYFGVDLKRIDSFICY
                     "
     gene            complement(63725..65197)
                     /locus_tag="K8M77_000147"
     CDS             complement(63725..65197)
                     /locus_tag="K8M77_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI7084678.1"
                     /translation="MAIKDANKQYIKFDTAVQVLKYEVLKRIAEKEFDGTLEKEKLNI
                     AKEIVDDLKPNVRCCIYKERAIVEERIKLALGGHENRENMIEVIDIACDECPVNRFIV
                     TDACRGCLAKKCRDSCNFEAINFDNRKCKIDYEKCKECGKCKEVCPYNAIAEVKRPCM
                     RACIPKALSYDVDSKKAVIDDSKCIQCGACVVDCPFGAIMDKSYLVDVIRLLKDEKKV
                     YAIVAPAISSQFNHSKIGKVITAIKKLGFEDVFEAALGADLVAVHECNEFKEKGELDF
                     MTTSCCPAFVSYIEKNYPELKEYISNTVSPMVAMARLIKSQNKDVKTVFIGPCIAKKT
                     EAKRNEVSGDVDYVLTFEELLALLDSRNIKIDECKESDTKHGSFYGRLFARSGGVTES
                     VKHLIDSEGIKVDFRPILGDGIKDCDIKLRLAKLKRAQGNFLEGMACKGGCINGPGSL
                     NHDIKNSKKVDKYGELSSSEKIKDTLVDIKFEDLNLSKNE"
     gene            complement(65266..65883)
                     /locus_tag="K8M77_000148"
     CDS             complement(65266..65883)
                     /locus_tag="K8M77_000148"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBI7084679.1"
                     /translation="MRLGRDFYNRDTLTVAKELLGKVLVRNINGVTLKGKIVETEAYI
                     GAIDKASHAYGGKRTNRTETLYADPGTVYVYIIYGMYHCLNLISEEKDVAGGVLIRGI
                     EPLEGIEEMSKLRYRKNYEELSSYEKKNFSNGPSKLCMALGIDKGENGINTISSEEIY
                     VEDDSSIKEDFSIVEAKRIGIDYAEEARDFLWRFYIKDNKFVSKK"
     gene            complement(66183..66494)
                     /locus_tag="K8M77_000149"
     CDS             complement(66183..66494)
                     /locus_tag="K8M77_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBI7084680.1"
                     /translation="MKEIIMDLIIHSGEARSYAMEAIQLAKAEDVEGARRLIEKASIQ
                     LGKAHKSQTALIQDEASDNKIEISMLLIHAQDQLMTTMTLKDLANEIIDLYEKLNQKN
                     F"
     gene            complement(66596..67843)
                     /locus_tag="K8M77_000150"
     CDS             complement(66596..67843)
                     /locus_tag="K8M77_000150"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBI7084681.1"
                     /translation="MNNELIIKGKKAKEASYTLSFASTNEKNNGLLKISESLIKRCDE
                     ILEENKKDIEKAIEKGTSNAMLDRLKLDEERVKSIANAVADVIKLDDPIGEVTSMFKR
                     PNGLRIGVQRVPLGVVGIIYEARPNVTADAAALCLKTGNAVILRGGSEAINSNLKIVD
                     IISDALKEAGLPEGSVQILEDTSRETATDFMRLNDYLDVLIPRGGAGLIKAVVNNATV
                     PVIETGVGNCHIYIDDEADINMGVDIIVNAKTSRPAVCNAAEKLLVNEKIAEEFLPVA
                     IKALKEKGVEIRGCEKTKVIVNDINLATEEDWGKEYLDYILGVKIVKDLDEAISHINK
                     YGTKHSESIVTKNYFNSEKFLQRVDAAAVYVNASTRFTDGGEFGFGAEIGISTQKLHA
                     RGPMGLKELTTNKYIIYGNGQVR"
     gene            complement(67846..68655)
                     /gene="proB"
                     /locus_tag="K8M77_000151"
     CDS             complement(67846..68655)
                     /gene="proB"
                     /locus_tag="K8M77_000151"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI7084682.1"
                     /translation="MRKALKDSKRIVVKVGTSTLTYENGSVNLMRIEKLSRVISDICN
                     MGKEVVLVSSGAIGVGVGKLRLEKKPETIREKQAVAAVGQCALMHIYNKFFGEYSHVV
                     AQVLLTRDVLENERMKNNVCNTFDTLLEKGIIPIVNENDAISIDEIQNILNFGDNDNL
                     SAIVSTLVNADTLIILSDIDGFYDSDPRTNEDSKMLKEVYEITPEIEECAGGAGSNRG
                     TGGMVTKLSAAKVATSNGIDMILANGENPEILINILNGEDIGTMFVAKKGE"
     gene            complement(68742..69620)
                     /locus_tag="K8M77_000152"
     CDS             complement(68742..69620)
                     /locus_tag="K8M77_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI7084683.1"
                     /translation="MSNEKLFPTTFPVQNQSIHPGVEDEMNPLPIYDLPQYINDGKRL
                     KNKVAIITGGDSGIGRAVALGYAREGAKVCIIYLSDEEDKDANKTKELIESAGSEALV
                     IKGDISDIDFCNSSVKKVIEKWGKLDILVNNAAVQHDSKTLDDIGPEQIENTFKINVF
                     GTFYMTKAAVKYMECGSSIINTTSITAYRGNEILFDYSCTKGALTTMTRTLATMLVKN
                     GIRVNAVAPGPIWTPLIPSSMNKKKVSEFGVNSPMGRAGQPVELAGAYVFLACNEASY
                     ISGITIHVNGGEIING"
     gene            complement(69684..70457)
                     /locus_tag="K8M77_000153"
     CDS             complement(69684..70457)
                     /locus_tag="K8M77_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI7084684.1"
                     /translation="MKKTVLITGATSGIGYEFSKIFMENNYDVILVGRNLEKLMELED
                     FSKKKCVMAYIYKFDLSLSENIDFLYKKIKTEVGKVDILINNAGIGFNGEFNEIDWQK
                     QLDVINVNIISLTKLTKLILKDMINEKEGKILNVASTGAYQPGPLILVYYASKAYVLS
                     FSEALREEVKDKGINVVTLCPGATKTNFSKRAGKGDLDVAMSAEKVAQCGYKALINNK
                     AICIPGIMNKVLVFLSKVSPTTLNAKIVKKIQNKAISKK"
     gene            70643..71935
                     /locus_tag="K8M77_000154"
     CDS             70643..71935
                     /locus_tag="K8M77_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBI7084685.1"
                     /translation="MNNFFKLKEHETNIRTELLAGLTSFFAAVYIIVVNASILSDGGV
                     SMEPLIIATVLASLIGCVLVALLSNAPLIIMPGMGINALFTYTIVNTMGLTFQQALAA
                     VIVAGILFIVVAVTPLADMLTSSIPTSLKEAITVGIGIFITFLGLQKAGLIVGDASTL
                     VKLGDISSKEVIVFFITMILMLVLFIKNVPGSFLIGILSGTVISMLFGIVNLSSFQLS
                     LPNFSEYKQVFLSANFSGIASISFWIATFSLTLVLVFENIGLLHGQIDGMLKQPSKRQ
                     RALNAVAFTTIICGICGTSPSVSTVEGSAGIAAGGKTGLTSIFTGFLFLISIFFIPVI
                     SLIPNSAIAPVLIIIGGLMIQNIVSINFEDFTESFPAFLTLTIIPLTFSIVDGIAFGF
                     IAYPICKLATKKYKDVSIPMYVISAIFLVYFVLHATQI"
     gene            complement(72000..73649)
                     /gene="hcp"
                     /locus_tag="K8M77_000155"
                     /gene_synonym="priS"
     CDS             complement(72000..73649)
                     /gene="hcp"
                     /locus_tag="K8M77_000155"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041707961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI7084686.1"
                     /translation="MSMFCFQCQETAGCKGCTVRGVCGKTEDVAKIQDLLIFVTKGLA
                     TVANEGRKVGIVDKKVNRMIIDNLFITITNANFDFKAIEKRVKDTLVAREELKERVQA
                     NGGNPIGSDFKGCATWTATTSEEMMEKASQVGVLATENEDIRSLRELIMYGLKGLAAY
                     MEHAMNLGHDKEEVHAFMAETLVKILDDSLSADELTALALETGKFGVDGMALLDEANT
                     STYGHPEITKVNIGVRNNPGILISGHDLKDLEMLLEQTEGTGVDVYTHGEMLPGHYYP
                     KFKKYAHFAGNYGNAWWLQNKEFASFNGPILMTTNCITPVQDSYRGRIFTTGAVGYEG
                     CIHITADENGHKDFSQIIELAKTCKAPTEIETGEIVGGFAHNQVLALADQVVDAVKSG
                     AIRRFFVMAGCDGRAKSRDYYREFAEKLPKDTVILTAGCAKYKYNKLPLGDINGIPRV
                     LDAGQCNDSYSLVVIALKLAEVFGTDSVNELPISYNIAWYEQKAVIVLLSLLHLGVKN
                     IHLGPTLPAFLSPNVAKVLVENFGIGGITNVEDDMKMFLGE"
     gene            73951..75300
                     /locus_tag="K8M77_000156"
     CDS             73951..75300
                     /locus_tag="K8M77_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBI7084687.1"
                     /translation="MKTKYKSSDLLKFVIPSLLGVILFMVPINDGGNITIPVAFFTTK
                     LKDLIGDYLPTLSMIVVVIAASLTIITKAFKPKFIIENNFLNSLLNVNWIWTLGRILG
                     GLFIISAFTGFGPEIIKSDLTGAFILNDLLPTLIVVFFFAGLFLPLLLNFGLLEFCGA
                     LLTKIMRPLFKLPGRSSIDCMTSWLGDGTIGVLLTSKQYEEGFYTEREACIVSTMFSV
                     VSITFSFVVLSQVGLENMFIPFYLTVTFAGIVAAITLPRVWPLSKKPDAYFNNAEPKN
                     VEDVPEGFTPFTFGVFKAVEKAQNESSLKKFFADGIKNVLEMWIGVLPVVMAMGTLAL
                     MIAEYTPIFQWLGVPFIPLFKILRIPEASAASQTVIVGFADMFLPSVIASKTILSDMT
                     KFVVACVSVTQLIYLSEVGSVILGSKIPLNLKELFMIFLMRTLVTLPVIALIAHLLF"
BASE COUNT        24053 a        13607 c         8430 g        29289 t
ORIGIN      
        1 ttctttttta cattcctttt ctttattctc ttcttttaaa taatccttgt ccttttcttt
       61 tagatactct ttatacttat ctttttcatc tttgtctttc ttgtccttat aataatcttt
      121 atcatcatta tatttttcct tatcctcttt catgttaata ggacattctc ttttcatttt
      181 gtcttcttta ttatttttat cgtaatgaga cttttctttg cctgatatca tcttaatctc
      241 ataatttttc caattatcct ttggcatttg attatttaaa aatccacaca taaagaatac
      301 tgttctacca ttaatatttt ttcctatagc agctcctact atattctcat aagatacatt
      361 taaatctcct atgttgatag aatcatattc taaactcatt tctgcctttc cttgttgatt
      421 tatgttcatt ggaccaagat ttatattctt cttaaaatct ttcttgttac aaataagcat
      481 catataacaa tctttgtatt ctttctttaa gttttgagcg taaaaagaaa ctttacattt
      541 atcattctta gtttctatct tggcatatcc agttaaaggc ttatctgaag ccatagaatg
      601 ccccttttca tcttcttgta atattataaa agttctatat gatttattat gtgccaattc
      661 tctggtccct ccaatataat tttattctct acagtatatg agagaacaaa aaaaaagaga
      721 ataaaaattt aaaaattctt attctctcta aatattctag ttattcatat atttatctat
      781 tgcatctgaa agagcaccaa actctgctaa gcttaaagtt tctcctcttc tcttaggatc
      841 tattccagcc tcttcataag ccttttccat taattcctta ggaagtttta catttttagt
      901 tgcattccat aaagtttttc ttctcatgtt aaatgcatgt ctaacaattt caaagaataa
      961 tttttcattt ttaactttaa cacttggctc ttctaatctc tctaatctta ttactataga
     1021 atcaaccttt ggtcttggta tgaagcatga aggtggtacc tttctaacta ttttagtatt
     1081 acagtagtat tgaactaaaa ttgataaagc accataatct ttacagttag gttctgcatt
     1141 cattctttct gctacttctt tttgtatcat tatagttaat gatttaaagt tatatccacc
     1201 ttttaataaa tttactatta taggagtagt tacataataa ggtaagtttg cgactaactt
     1261 aacgcttttt tcatctccta taatttcatt aaaatcaact tttaaggcat cattatgtat
     1321 taattgaaac tttggattat ctccaagctc agctgttaat attggaataa gcgaactatc
     1381 taattcaata gcaacaactc tctttgctct tttaagtaat tgtacagtaa gagtaccaac
     1441 tccaggtcca atttctatta ctaaatcatc ttcacaaaca tctgctccat ttactatatc
     1501 tcttggaaca gaatcatcaa ttaaaaagtt ttgtcctaag ctttttgaaa atctaaaatt
     1561 atatttctga acaagctcct ttgtctttat atcttttatt tcgtttatat ccattcacta
     1621 ctctcctata tcatcttttc tatcttttct atagctttaa caaattcttc ttttgttatg
     1681 ccatagttat ttaatcttga aagaacttgg tttgcatttc cataaccgat tcttaattct
     1741 ttaccaagta actctcttct cactttagcc tttggatctg ctgataatcc aaagtatatt
     1801 agatcttgta ttgtaaattc ttctcttttt tcttctaaag ttatttttgc attttctaag
     1861 gccctaatta tagcctctgg tgacgcattc tctacgccta tgtttccatc cttagctcta
     1921 gttccctctg ctcttcctat gtaagcatgc ttaacgcctt taactctctt tgagattatt
     1981 gatcttattt tttctccagc aaaatctgga tcagtaaata caataactcc ctttcttttt
     2041 tgagcttctt ttattctctc tataaccttt gcatttattc cgaatccacc aacagctatg
     2101 atttcagcat ctatagcttt cttaactgct gttatatcgt ctctaccttc aactactata
     2161 acttctttaa tcaaggttaa aactcccttt cttattatat gtactatatt taatttacct
     2221 atatctcatt taactgtcaa taaaatgctt taatacaaaa taaatagagg gtctaaaaga
     2281 atactctttt agaatccctc tcttagtatt ggcattacca ttcaccagga taagctatta
     2341 tataaagatt aacatttctt cttccccact gtttacattc aggtgctgaa tttaagaata
     2401 aatctattat attatttttt atagctccac cagtgtcttc tgctactgca tagccatatc
     2461 cttctacata aaccttagtt cctaaaggta ttactctagg gtcaacagct attgtactta
     2521 atccatttgg atctctctta ggtacagttc ctgtagctgt tattccatct ccagaatagg
     2581 cagtagcttc taccaccatt gattttttat aatttatatt atcctttcct cttgaaagaa
     2641 ctaatttctc catagttcct ttaaccacca cttcatctac tggttcttta acaactttag
     2701 attctactac ttctctagat acttcttttc cattttccat aaccaacttc atagttattt
     2761 ccttttctcc agaagctccc tcttgagtag tttttttaac tccactctct aagctattat
     2821 cttcctttac tacagtttca aagtctattg gctctttttc aactatggtc ttctcttcaa
     2881 ctcttgtaat ctttattttc aaatcctttg atagttctgt ttctaatgaa ggttctacct
     2941 tgtctaagtc atttagatta attccttctt taaacaacat attctttact gaatcttccg
     3001 cagaatctat ctctatattt tttccatcaa ccattacttc tactggaact gccttcttaa
     3061 tgcttattac atcattatca gcaatctcgc ttgttaaact tggttctatt ttatctttgt
     3121 cagataagta aattccttgt tgactcaaaa ctgcttgtac atttccttta aatgtggcaa
     3181 catctaaatt ttcaccatct aagtttatgg ttatgttctt atgtgctgta actaaaacta
     3241 ctataaaact tactaaaact aaactaaata ctcctaatac aatttttcca tttttcgagg
     3301 caaccttctc tttcaatgcc ttaattattt ttttcatata atcttccccc ttttaggcaa
     3361 gagtcattcc gacattatat attacctttt aaagtatgtc aaatctgatg actcctaaaa
     3421 tttatacttt tagttgtaaa ttataataaa taattgacct ctgcttatac agggatttgg
     3481 taaaaaatag ttaatttata tttaaaaagt acactatatt atattagtgt actttccata
     3541 ttattacatt tatttttctt tagtttagtt gtagttatat taaattttga ataatctttt
     3601 tttattgttt aataagattt cattcatttc ttcccctgaa attcctctta tttcactcat
     3661 tttatctatg atatattcaa tataatctga cttgtttctt ttgcccctaa aaggaactgg
     3721 tgccataaaa gggcagtcag tctcaactaa cattctttca gcaggtattt ctctacaaac
     3781 atctactaat tttttagcat ttttaaatgt taaaacccct gtaaatccaa tgtaataccc
     3841 taatttaatg cactcttttg caaattcaac tgaacctgaa aaacaatgta ctactccaat
     3901 tacatttgga aactccttca taatttctaa agtgtcttta tgagcatctc tatcatgtat
     3961 aacaactggt aaatttaatt catcagccaa tttcatttga gctctaaaaa cttctttttg
     4021 aacttctctt ggaggatttt cttcccaata ataatctagt cctatttctc caatagcttt
     4081 aaccttttca ttcttaacaa attcttttat ttcttttaca acatcctcat taaattcatc
     4141 tgcattttct ggatgtatac caactgcggc ataaaacata tcaaactctt ttgcaagttc
     4201 aactgatttt cttaatccat aaagatcaga tccacaattt aaaaccccaa taactccatt
     4261 ttcttttagt tcttttatta cattttctct atcttcatca aaactatctg agtcataatg
     4321 tgcatgtgta tcaaatattt tatacttcat aattttctcc tttgaatttt acatataaat
     4381 tgtagataaa agtggtgata gaattcctag aattcctcct aataaagctc ctaaccaagt
     4441 tattgcctta agttctttat tagctattcc aattatgatt tcctctgcat aatccacttc
     4501 aaaagcattt atttgctctt ctactattga ggaaatatcc acaatttcta gtactttagc
     4561 tgatttattt tccacaaaat cattatataa attatctaaa atactcataa tactattttc
     4621 taagttttgt ttattataac atatatcatt taaggaattt tgtaaaaatt tatcaaatag
     4681 cttttcaatt attttactta ttccactagc taattggtta ttattaacaa tttgagaaat
     4741 cattgaatct atattatcaa ttagaatatt ttcatagtca ttgaataaca cttttataat
     4801 ttcttcataa ctattaaaag aagaaatttt acaatttagt tttcttatta tagaatctat
     4861 attttcttct gtatttattt tttttgaaat cttatctcct aaggcatctg atattacctc
     4921 tagcttttct tctccaacag tatttattac atcacttact tttttcttca tactttcatg
     4981 tacaaaagct aaagccgcat cacatatata ctctttattt tcttcttcac ttaaagattt
     5041 atcaactaat gaaactaact ttccgtatat tacatctcca cttaaaaaca ttgatactat
     5101 ggatggtata ttatcattta ttgcactttt tatcttatgt gaaacctcat tatctctaag
     5161 aagatttttt attatatcta caagagtatc tttttgagat tttacatatt cttctatata
     5221 aggtttaata ttttctggaa ttacttcttc aatagttaaa tcttcttttc ctaaagtttt
     5281 tacttctttt aaaagagctt ttcctattat gtctcttgtt ttttcttccc ctaaagtatt
     5341 tattattact tctctaaatt tattgctctt tataaaatca attatctttt caggctcttt
     5401 atttaatctt tctttaatag aatctactat ataaaattta accttatttt taaactcctc
     5461 ttcctgaatt gattctaaaa tagtcttagt tatattgttt atcatatttc cctttagagc
     5521 atagtaattt tcccctaaag tattttttaa tgattcctct aaagttgaat tgctattaat
     5581 aacttgatta attttttctt tagcaacctc attaaatttt gcttttattt tatcatcctt
     5641 taaagctttg cttaagctat ctgaatttag taaatgagtt cctacagttt ctccaacctt
     5701 attcgctatt ctactttttt ccttaggtat taaccctggt gtaaaaggaa gtttcatgcc
     5761 aaagatatac tttgcttctc ttggcctaaa taacattttt atagcaagcc agtttgttat
     5821 gtagcctatt actgccccaa ttaaagctcc tataatataa attttcattt attttcactc
     5881 ctatcaattt aaaattcact taaatagtgt aaaatattat atattaatat aatactactt
     5941 attttttaaa ggtggtgaat aaaatggatt taaaaaaatt atctaatata aaagagtgta
     6001 ggttattttc ccacttcagc gatcaagata ttcaagaatt attaagctct ggtttcgctt
     6061 cagtaaaatc ctattcaaag gaagatttac tgttttctga aggagaatat tgcaattttt
     6121 taagtgtagt tatagaaggg aaaatagaaa ttcaaaagtt agattctaac ggaaacatat
     6181 tagtggttgc aaccttaaat agaggaaatg tttttggaga aaatcttctt tttggagata
     6241 agaactttta cccaatgtct gttgtggcta aagaaaattc tcaaatttta catataaaaa
     6301 aggattatat atataagcta tgctctttaa atgaagcttt tttaagagag ttacttagaa
     6361 ttctttcaaa taaagctctt aatctaagtt ctaaacttaa gcaagtaaca atgaaatcta
     6421 ttagagaaat gatttgcaat tacttattaa aagaatataa taaagaaaaa tctacaacta
     6481 taaagttaga gttttcaaga aaagactggg ctgacaagct tggagtccaa cgaccttcat
     6541 tatctagaga gcttatgaaa atgagagatg caggacttat agaattttca aaaaacataa
     6601 tagaaataaa agatttagaa gggctaaaag gttatttact ttaaatcaaa tgttaatatt
     6661 tttgtacact ttttgacaca taaatgccac ataagaagct taatataata attgtaacgt
     6721 agctaaaaca ctaatttaat tttacccctt aaatggccct tgaatagttt gtccccctta
     6781 cgcaaatgtc aagggctatt aagtttaatt aattaaaaaa atttctatat aataattcct
     6841 aaccttaaac cttaagatgt agaataagcc ttgtttctag attaatagaa gcaagactta
     6901 ttttatttat atctttttat tttttaatat atttaattct tctatataag ctttcttgtc
     6961 tattttatca aataaaaatt caactctatt aactcttata ttcttcctct cttcaaagct
     7021 ccactttgta tcctttatac ctaaaaacat ttttattttc tcacaagtaa aaggaattat
     7081 aggattcatt aaatcagtta aagctactaa tatttgagca caaatataca gtacctcttc
     7141 acattttttc ttgttattat ttattaaaat ccaaggttta tttttatcaa agaaatcatt
     7201 tccctgtttt ataagattaa atatttcttt tactcctgat ttaaattctc cattttcaaa
     7261 tttattgcct acagaagcat acgttcttat tattatgttt ttccattgaa gaggaagttc
     7321 ttctctcttt atttccccat taaagttttt ctttataaag gttaaaaccc tatttgtaaa
     7381 gtttcctagt tctcctacta attcattatt atttgcattt ataaaatctc tccaagtaaa
     7441 gtcagaattt ttctcttcag cacctctact tattaaataa tatctaatag aatctatatt
     7501 atatctttcc ataatataag gaacccatat ggcccaattt ctattggtag aaaaagtttt
     7561 cccttctaag gttaaatatt gagaagagaa tattctaatt tcacttttct ctaatccaat
     7621 gccagagagc attgctggaa acattgttgt gtgaaatggt atattttcct taccatgaac
     7681 taaatatatt ctactatctt cattgttcca atattcttct aaagattgcc ctttttcctc
     7741 tataactttc ttagatgcag taacataggc taacagtgca tcaatccaaa cataaaccct
     7801 tttatcttca aagccttcca caggaacttc tattccccaa ttaatttctc ttgttagaat
     7861 cttatctctt aacccaccat ctaagtatct cttagttatt ttaatggcat tttctctcca
     7921 actctcttct tcatctaata tattttgaat ataatcttga aaataagata atttaaagaa
     7981 aagattatta gaacttctaa cttcaacttt gctattacat tcattgcaaa gtccatcttt
     8041 aactccaaat tcatctaagg tatcacagca tttttggcaa taaaattctt ctgtttcttt
     8101 ttcatatata aaccccttat cataaaggct ttttattatg ttttttacct gcttttcatg
     8161 atactcacta tctgttcttg taaatatatc aaaggaaaat cctaatttat taaaacatct
     8221 aacaaattcc ctatggtact tatttataag ctcttctggt gacttattaa tttcctttgc
     8281 tttatttaaa attggagatc catgacagtc agatcctgaa acaaaaataa cttcatcacc
     8341 cttagcccta tgataccttg ctaatatatc acctgccatc caagatgaaa ctcttcctat
     8401 atgaagtgag ccgttggcat aaggccaagc attacctata attactttca ttttattagc
     8461 ccccttaaaa tgcttctatc tacaatatta tgatacaaaa aaactcttat attccacaat
     8521 ctagtatgaa tataagagtt ttttaatttt actatcttac ttggcttcca gttaacattt
     8581 ctcctgggtt aactaataca agttctgaat catctgaagg tgctgctgca agtatcatac
     8641 cttgagataa ttcccctctt aatttaactg gttttaagtt agcaactaat actacatact
     8701 ttccaactaa ctcttctggt ttatagtatt gtgctattcc tgaaataacc tgtctttctt
     8761 ctccacctaa atcaacctta agttttaata actttttagc ttttttaact ggttcacatt
     8821 ctaatacttt aactactctt aaatctatct tgtcaaagtc atctatagtt atttcttctt
     8881 ttattggatt tttaactaac tcttcgttag ctggtttaac tggagctggt tttaaagctt
     8941 caagttctgc taatttttcc tcaacatcta ttcttgggaa tataacatca cccttaacta
     9001 ctttatctcc tgcaactgtt ccattgaact cttttaaact ttcccaagtt gtaactttag
     9061 tgtttaattg atcatttatt tttacacttg tttctgttaa gaatggtgaa atcattactg
     9121 aaacaaatct taaagtttct aataagttat ataaaactgt tcctagtctt tctttctttt
     9181 cttcatcctt agcaagtatc caaggagttg tttcatctat atatttatta gctctgctta
     9241 ttaaagtcca tatgctttct aaagcttctg gaatctttaa tgcatctatg ctagcttcaa
     9301 cttttcctgg agtagataaa gctaagttta ttaattcatc atcaatagct tccttacatg
     9361 ttggtgcttg aataactcca tcaaaatatt tataaaccat agctattgtt cttgataata
     9421 agttacctaa atcattagct aagtctgtat ttactttctt tataaatatt tcattgttga
     9481 ataatccgtc tgaaccaaat ggtatttctc ttaataagta atatcttaca gcgtcagctc
     9541 caaacatatt aactaaaact actgggtcaa caacgttacc ttttgatttt gacatttttc
     9601 ctccatcaac aagtaaccat ccatgaccaa atacttgctt aggtaattct aatcctaaag
     9661 ccattaacat tattggccag taaattgtgt ggaatcttaa aatatctttt cctattaagt
     9721 gaacatcagc aggccagaac tttttgtata actcttgatt ttcttgacca tatcctaaag
     9781 cagttatgta gtttgataat gcatctatcc aaacatagat aacatgcttt tcatcaaagc
     9841 ttacaggtat tccccaagta aagcttgttc ttgaaacaca aagatcttgt aatcctggtc
     9901 ttaagaagtt atttaacatt tcattctttc ttgattctgg ttgaatgaaa tctggatgtt
     9961 cttctatata ctgaattaat ctatcagcat atttgctcat tttaaagaaa taagcttctt
    10021 ccttagcttt ttcaactggt cttccacaat cagggcagtt tccatttact aattgagttt
    10081 ctgtccagaa tgattcacat ggagtacagt aaagaccttc ataagaatct ttatatatat
    10141 ctccttgatc atataacttt ttgaaaatct cttgaacagc tttcacatga taatcatcag
    10201 ttgttcttat aaatttgtca tagcttatgt tcatcatttt ccaaagatct ttaattcctg
    10261 caactatttc gtcaacatgc tcctttggag taattccttt ttcattagct attctttcta
    10321 ttttttgacc atgctcatct gttcctgtta agaacattac ttcgtgacct gtaagtcttt
    10381 taaatcttgc tatagcatca gcagccacag ttgtataagt attacctatg tgtagatttg
    10441 ttgatgggta ataaataggc gttgtaatat aatatggttt tttacacatt atttcttcct
    10501 ccttgtattc ttacttatta ttgtttccaa tatttcaaaa atataaaaaa cccctatggg
    10561 aaatcccata gaggcgttaa ttacgcgata ccactctatt tcatacttat gtcaccataa
    10621 atatctcact aagtaactcc taagtttaac ttattttatt actctgcaat ataacggttg
    10681 cccccgtact tttctaaaat taaaattctc aaaaagtctg ctccaagtcc atcttcataa
    10741 agtctcatgt atctggcttt cacctacccc agctctcttt tatacaatct tctctattac
    10801 tcttctcttc atcgcatttt ctaacttaat tttaattata tgattagttt tatattaatg
    10861 tcaatcgttt ttatctttga attctttgaa attcttgtta aatcataatt tgtattaata
    10921 taaaaatgtt atatactatc tataataata tataaattgt tggggaggta ttatttaaat
    10981 gaaaggttta gccctaccta aaataggtgc aagaaactta aagacagctt tggctgtaac
    11041 tttatgtata ggatttttcg agctaataca tagaccatat cctttttatg cttgtatagc
    11101 cgctgtaatc tgtatgaaag atactgttga aaactcatat aaaatgggaa agagcagaat
    11161 gattggtacc ataactggtg gacttgttgg cttagcttta acttttatag ctttaaactt
    11221 taaccttaca agattctcag gaataataac agggattgga atagttataa ccatttatct
    11281 gcttaatgtt tttaacagaa aagggtctgt ttctatagca tgtatagtat taatagcaat
    11341 tatgacaaac ttacatggta aagctccata tgcctatgca gttgatagaa ttatagatac
    11401 ttttataggt ataataattg ctctattaat aaataactac atatataaat atgaaaataa
    11461 ggtaaaaaaa gatttagaaa atatagaaga aaaaatctct agacttgaaa aagaagttaa
    11521 agatgatata aaaaatctag aagaaaataa aaagaactaa tgtaaaaaac taatatgatt
    11581 taggagggaa atatttgttt aaatttaacc tgcaaactat aggtgctaga aaccttaaaa
    11641 cttctctttc agtatttata tgcattatag tatttgaact attaggccgt tctagtgcct
    11701 tttttgcttg tattgcagca atgatgtgca tgcaagatac agttgaaaat tcatataaaa
    11761 tgggaaaaaa cagaatgctt ggtacatttt taggggctat tgttggttta ttattaacag
    11821 ccatatttaa tgtattaagt attcaacact ttattgttta tgctctttta acagccatag
    11881 gtacaactat agccattttt ttatgtattt tatttaattg taaaggagca ataaatagtg
    11941 catgtatagt tgtttttgcc ataatgacaa acttaaaggg tgttggctca tatgactatg
    12001 cttttaatag aatactagat acatttattg gggtaataat agctattata attaacagac
    12061 ttgtctatcc ttaccaaaag aaaactgaag agaaaacaaa ttaatatatt taactaaaaa
    12121 agagaaggaa atccttctct tttttaattg ctaactactt tgtcatctac ttttttacca
    12181 aatatcattg ttaatatgaa taaagctacc aaggataaag gtaacactat gaaaatactt
    12241 aatcctaaac cagctggaat atatccaaat aatacagtta ctattcctac aaatactgcg
    12301 taccatattt gagttctaac atgttctata tgatcgcatc ctgcacctgt tgaggaaagt
    12361 attgtggtat cagaaatagg tgaacaatga tctccaaaga tagcacctgt taatactgct
    12421 gatgtgctta ctataacaaa attccattct ggatttattg caaaggctaa tggaattgca
    12481 agtggcatta atattcccat tgttccataa gctgtacctg tagcaaaggc tattattgaa
    12541 cctaaaataa atattattgc tggtaataaa aatgctggta ttgcatctga taatactcct
    12601 actaaataag aagcagttcc taattcttta attacagaac ttaaagacca agctaatata
    12661 agaattactc ctgttattaa aagtcctttc attccttcaa tccaaattcc tattccttct
    12721 gaaatagtaa agatttttct agaaactcct aatataagag ctactatagt tgctaataaa
    12781 gctgattgga ataaaactac ggaagcatct gcatttgaga atgctgcttg tattccagca
    12841 aaactaaaag gtgtattgtt cataagctct attattgcta aatcttctcc acccattata
    12901 gttacccatc cactataata aaatcctatt aaagatgata ctattaaaac tccaattgga
    12961 attattgcat tccaaacact taatttaatt ccttctatag gttctaaatc ttcactatca
    13021 gatgaaattt ctgtagtaga taaaacttct cctcttctag ctcttctctc agctttaagc
    13081 attggtccaa actctcttaa agttattgct gatataacaa caaaagctaa aattaatata
    13141 ttataaaatc tatatggtat tgtttctaaa aatactccaa aaccatcagc ttgtattcct
    13201 acacttttta aagcatctcc aattaatcca acctctaagc ctatccaagt tgatattatt
    13261 gctaaccctg caattggagc tgccgttgca tctataataa aggctaattt ttcccttgaa
    13321 actcctaatt tatctgtaac tggtctcatt attggtccta caattaatga gttagcataa
    13381 tcatcaaaga aaactgctaa gcctaaaagc caagtggtaa gttgtgcact cttaacagtc
    13441 ttagcctttc ttgctaaagc ctcagctatt gcctttgctc ctcccatttt agctactaaa
    13501 tgaatcactc ctccaataac taaaacctga agaattatcc ctgcattcca aggatcagct
    13561 aaggaattta aagctctata agtaaaatca ataaatcctt gagttaacgc tgtaattata
    13621 ttaaatccat ttaattgaag cattatacat cctgtaattg ttcctatgaa taaagaaact
    13681 acaacattct tagttaaaaa agctaataca attgaaacta atggtggcag cactgtccac
    13741 attccaaact tagctgagtt taccgccgca gcatcttcag cagcaaagac taaggttgag
    13801 cataacattg tcattgcaaa aaccgttaga aaaactttaa atctagtttt gtttctcatt
    13861 ataaattccc ccttaaatat gcagtgaatt aaattacaga gtttttccct tattttctct
    13921 tattataaaa tttctgtaaa aaagacctta ggcttgatca acctaaggtc ttaatactta
    13981 ataaaattac ttttgcaaat aaacttttat ttaattccct aagatgatag ctctccacat
    14041 tttgtgacag tgttgtatat attctataca accccagcat ttagccttaa agtattagcc
    14101 aaaaacttcg gcgataatcc ctttcctaaa acttctacgc tacttaactc cattttagta
    14161 atcttgattc tcgcgcctct acctcttttt aaattattca ctttttaatt attatattgt
    14221 atacatttat acaatataac tatatttctt tcaagtattt aatcacttta ttaactattt
    14281 attaaaaaaa gcctgatttt attatggaaa tcaataacca caagccaaaa attcctgata
    14341 ctataaaccc tattaaccct aaaataggaa ccccttgaaa agtaggtcca gttttagcat
    14401 taactattaa tgaagaacct attatcatac atccaactat taaagagaat gctattctat
    14461 ttaccattct atttaaatct gtaagtggct tatctaatcc cttcatttca aattgcaact
    14521 tagctcttcc cttagttaag ctatccacaa gttctgaaaa tttagtagga atttctacgc
    14581 tcttagaagc aaacttatag gcttcagtaa taagtttatc cttgtctaaa ctttttagca
    14641 tttcctcttt atttctttcc tttacataag gataaataca ttccataatt tctaaatctg
    14701 gatctatttt agctataacc ccttctaata taactatgga tcttactaaa gcaactaatt
    14761 ctcttggaaa ctgaatatca tttcgttctg ctatctcaaa gatttcttta aataaatctg
    14821 atatttttat tgtttttaaa gatgcattta tatattttga gactatattt tttaaatcat
    14881 catacaatac tgaatatcca acttttcctt tttttatgcc tatggcattt acaaaatcaa
    14941 ctaaagtatc aatatcacct aaaaccatag caatcatagc cttattaagc caatttttta
    15001 aatttgcttc taaagttcca actaagccaa aatctataaa gtatattttg ccttctctta
    15061 taaataaatt tcctggatga ggatcaccat ggaaaaatcc atcttcaaga acctgtttaa
    15121 aaaatgagtt tgctaatttg tttgcaatat cttcaaagtc atatccttct tcttttaaaa
    15181 tattaaaatc tgttacttta caaccatcta tatattccat ggtaaggact ttcttagaag
    15241 taagcttatc aataattata ggtgcaccaa cacaggctac atttttatta agctctctaa
    15301 atattttagt attctttgct tccttttcga aatcaagttc ctttaatgta gcatcttcaa
    15361 cttccttaaa agcttcaact ggattaatta aggtattagc tatatgatta gatgttaatt
    15421 tactaagtct tcttaatatg cttaaatcaa gcttcatttt ttcatctatt ttataatgct
    15481 gaaccttaac tactacatct cttccatcta ttaatttagc tctatgaacc tgtgcaattg
    15541 atgctgatgc taatggagtt tcactaaact ctaagaaata tgtatttata tcagtcccaa
    15601 aagattcata aaatatttcc tttaccttat gaaagtctgt tatttcatta ttatcctgta
    15661 acttttccaa ctcttcaata taagctttag gaagtaaatc tggtcttgta ctcaatattt
    15721 gtccaatttt aacaaaggtt gcccctaatt cttctaaagc ctctctaagt gcttgaggcc
    15781 ttggctcagc ctttttacta ttgggcaact ttgatgaaat taaaaagtca aaaccataat
    15841 aagtaaatac tctaactatt tctttcaacc tttttgcact ctgtccctta ctcataaatt
    15901 cacctcctat aagataataa actataaaaa ctctacaagg cactgaaaag cttaatcata
    15961 ctagctattt acatagctat aaattctact cttcattttc cttgtagagt tctattattt
    16021 tatagcctaa aattcttctt catttaattt agcttctaat acggctattc tttcttttaa
    16081 tttatttatt tcttctaaag tagatttatt agcttcctca aacataactc taaaatcttc
    16141 ttttgtgaaa ggtttcatat tgtctaattt atttattatt tgacttgttg cttcagtagt
    16201 tttttcttgc atatctcttt ttaattcttc agataaaact ttaccatctt caacagttat
    16261 tttcccctta tcaaccatat cttgtataaa agaatctgtt ttttcataag cagtggctgc
    16321 agttccaatt ccagctaaga cagctttttt taattcattt atcatgaaat catctcctaa
    16381 accatagttt actctttaat ttttattaca ttttactctt aatttcttca aatttaaagc
    16441 ttattattct taaaataatg ttaattataa aattatctat tactcatgaa cttttctata
    16501 tcttctacag acttaatgca ttcttttgtt aaaacttcac atccatcttt agtaactaaa
    16561 acatcgtcct ctattcttat tcctatagct tcttcttcaa tatataatcc tggctctaca
    16621 gtataaacca taccttcttc aagaatgata tctctcttac caacatcatg agtgtcaaga
    16681 cctaaactat gtcctattga gtggaaataa tactttctat aatctttttt gtcctctata
    16741 agacctaatc ttacacaagc ttcagctaaa agattatttg ctttgtcatt tatttcatag
    16801 aatccaaccc ctggtctaat agagtttatt atctcttcat ttacttttaa aacttcttca
    16861 taaacctctt tttgtctctt agtaaactta ccattagcag ggaatgttct tgaaatatct
    16921 ccattataat aatttacttg agcccctaaa tcaaaaagaa ttaagtctcc atcttttatt
    16981 tcactattat tatcaacgta atgtaaagta gcagcattta cgccagcagc tgctatagtt
    17041 ttaaaagcat aatctttaac tccattttgt tttaaataaa aatcaaaata tgcttctaat
    17101 tcatattctt tcattccagg cttacattct ttcattaaaa gttctacacc tttagctgtt
    17161 atgtgagcag ccttctttat ttcttctact tcttctttac atttaatttg tcttaaggaa
    17221 gctatatcag aataaacatc ttttataact acttgaggat atctctcctt aatttccttt
    17281 gcaaaacttt gacttttact catttcttca tcaaatgaaa ctctttctaa atctaaataa
    17341 agattttctg caattccatt agtaaaaatc atatttaaat cacttttaaa ctcttctaaa
    17401 tattttatat tttctatgcc agatacttct ttagcttcat aatctcttat agtctttcca
    17461 gtccatcttt cttgctctaa atttggtttt agtatataaa ggacttcatc aaccacacca
    17521 tttattttct ttatcattag tatatgcttt tcttcatcca ctcctgttaa ataataaaaa
    17581 tttctgtttg gagtaaatgg aaaatattca tctgctgttt ttcttttagc ctctcctgca
    17641 aataaaacta ataatgaatt gtcttctagt ttctttaata aattctctct gtttttagta
    17701 aaaactaaac ttttcatttt aatccctcca attcctattt attaaatttt accatatata
    17761 agatttaaaa tcttaacaaa taatacatct ttacatattt ttatagaaaa aaatactcac
    17821 taggttatct agtgagtatt tataattatc ttatttcaaa gctattaaac tctgtctttt
    17881 ttaccttcga aagcattgat gtacatttgc ttgatttcac ttattaatgg gtatcttggg
    17941 ttagcaccag tacattggtc atcgaatgca tcttcactca ttttttctaa tgtagcgtag
    18001 aatgcttctt ttgaaactcc acaatcagct attgttgcag ggattccaac tgttttctta
    18061 aggttatcta tagccttgat taagttatta actttttctt cttcgttctt tccacctaat
    18121 cctaagtagt cagctattac tgtatatctc catttagcat ttggatattt gtattgtggg
    18181 aatgcagctt gttttcttgg gttgtcaaca gcgttgaatc ttattacatg gtctattaag
    18241 attccgttag caactccgtg tgctatatgg tgttctgatc ctaatttgtg tgccattgag
    18301 tgacatactc ctaagaatgc gttagcgaat gccataccag ccattgttga agcatgagcc
    18361 attttttctc ttgcttttat gtttgtagta ccttcagcat aagcttgtgg taaatatttg
    18421 aatattaatc ttatagcttc taaagctaat ccattagtgt attctgatgc taaaactgaa
    18481 acgtaagctt ctaaagcatg agttaatgcg tctatacctg atgcagctgt taatcctttt
    18541 ggcatattca tcataagctc agcatctatg atagccatat ctggagttaa ttcataatct
    18601 gctaatggat attttactcc tgattcttca tcagttataa ctgcgaatgg tgttacctct
    18661 gaaccagtac cagctgatgt tggaactgaa atcatcatag ctttttgacc catagttggg
    18721 aatgggtaaa ctctctttct tatatccatg aatctcattg ctaaatcttc gaaagcaact
    18781 tctggatgct catacattac ccacataatc ttagctgcgt ccattggtga acctccacca
    18841 agagctatga ttgtatctgg ttgataactt ctcattgctt cagcaccttt tttagcacat
    18901 tctaaagttg ggtctggagt aacttctgaa aatactctaa aatctacacc tatttcttct
    18961 aaaacatttg ttattttatc aacatatcct agactagcta atacgttgtc tgttactata
    19021 aatacttttt tcttgtttaa atcttttaat tctctaagag caactcctaa agcaccatat
    19081 ttgaagtaaa ctttttctgg aactctgaac caaagcatat tttctctcct ctcagctaca
    19141 ctcttaacat ttattaagtg tttaggacca acgttttctg aaactgagtt tccaccccat
    19201 gatccacaac ctaatgttaa tgatggtgct agcttgaagt tgtatatgtc tccgatagct
    19261 ccttgagttg atggcatatt tattattgtt ctaccagttt tcatagttgc accgaatttt
    19321 ttaactctat cttgtgattt cattgtatca gtgtataaaa ctgaagtatg tccgaatcca
    19381 ccaagttcaa ctaatctttc agctttaact aaagcttctt caaagttagc tactctgtac
    19441 attcctaata ctggtgataa tttttcatgt gagaatggtt cttctaattc aactgactca
    19501 acttcaccaa ttaaaacttt agtatctttt ggaaccttaa cacctgccat gtcagctatt
    19561 ttccatgctg attgaccaac tatattagca tttaagttac catttattaa aattgttttt
    19621 cttactttat ctatttcttt atcttttaag aagtaagctc ctctatcagc aaactctttt
    19681 cttacttcat cataaacttt atcaacaact aatactgttt gctctgatgc acagatgaca
    19741 ccgttatcga aagtttttga taataatatt gagtttactg ccatttttaa gtgagctgtt
    19801 tcatctatga ttgctggtgt attacctgct ccaaccccga tagccggttt tcctgatgag
    19861 taagctgctt taaccattcc tggaccacca gtagctagta tgatatccgc ttctgccatt
    19921 aatacttgtg acatttcaac tgatggctca tctatccatc cgattatatc ttttggagct
    19981 cctgctttaa cagctgcttc taaaacaact ctagctgcct ctattgttga gtttttagct
    20041 cttggatgtg gtgaaaagaa tacagcgttt ctagtcttta atgatattaa agctttgaat
    20101 atagctgttg atgttgggtt tgtagtagga acgattgctg ctactacacc tattggctct
    20161 gctattttag tgataccaaa tgattcgtct ttttctataa ctccacaagt tttttcattt
    20221 ttatatttat tgtatatgta ttctgcagca aaatggtttt ttataacttt atcttcaact
    20281 atacccattc ctgattcttc aacagccatt tttgccaatc tgattctttc gttattagct
    20341 gctactgctg cttgtctaaa tatttcatct acttgctctt gagaaaattt tgagaactct
    20401 ttttgagctg ctctaacttt ttctaatcta agcattaatt catctacatt tgttactttc
    20461 atgataccca cctcattata gattagtttc ttattttcat aatatatgta aaaattaatt
    20521 aaaagcattt agttaagttt ttaactttaa taaaattcca tttaagaact ttttttcata
    20581 tctttttttg tttttaagtg ttttaactgc tctgtaaagt tttatactaa ccttacgaga
    20641 ttataatacc atctttcaaa atcaaaatcc agactttttt ttacattttt tacattttat
    20701 caactaaaat tagttatttt tttaactatt tcgttatttt tttatcaact ttaaatattt
    20761 acattttagt taaacgattt ctggaatata ttgtattatt tctgtagtga tttcatattt
    20821 aaataaaatc atcttttgtt ctcctttttt ttcaatagta tgttactatt tttattattt
    20881 tattcattta aagtccaaaa aacatactta ctactaatac agccattata atagcagtta
    20941 tatcagccat taaagcggcc caaaaagtat gccttatttt ctttattcca acagccccaa
    21001 agtaaacagt tatggtgtaa aatattgttt ctgttcctcc cattattaca gaagaaatta
    21061 atccttcctt tgtatctggc cctaaggtat ttattaattc tgtatatact cctaaggctc
    21121 cacttccaga tagaggttta attagtacta gtggaacaat atcttttggt aatcctatta
    21181 gagaaactat tggctctaaa agattattta aataattaag taaattagca tctttaaata
    21241 ttcttatggc aatcatcata gctaataaag caggaaaaat tttaacgcat accattagtc
    21301 cttcttttgc cccttctaaa aaccattcat aaacttttct tcccttaaac attccataga
    21361 ctattattaa aagaaatatt attggaatta tgttatttgc aatcaaaagt atccctccta
    21421 aaaatatctc tgcaagattt tacagcatat gattcctaca cagcaggcag taaaagtaca
    21481 tataatagca ggaattatta tacttgcagg ttcacttgat ccagcagctg ctctaattga
    21541 tataactgta gatggaatta actgaataca agctgcattt ataactaaaa ataatgccat
    21601 atcatttgat gcccttccat ccttttcttt atttatcttg tttaattcgc tcatagcctt
    21661 aattccaagg ggagtagctg cattagatag tccaaacata ttagcagtta agttcataac
    21721 tatagctccc atagcctcgt cactatgaga actctcctta aagatctttt ttaatatagg
    21781 gtttaatagt tttgcaacct tagatgttaa tccactgttt tctgctatat tcattactcc
    21841 acaccaaaaa cacataattc ctactaagga aattataaat ttaacagcac cttcagaaga
    21901 ttctgttaag ccagttgtaa tcaactctcc ctggcctgtc attatgctaa aaatcaatcc
    21961 taaggctata attgcaaacc atatataatt aatcatctct cctcctaaat cataagtaat
    22021 ttctgtataa tatatgcctt gcatagttag aatagtagtg ttaccataaa actacaaaaa
    22081 acgaatttac ttaaaaggag aattttttat atgaacgaaa aaaatttaaa attagctcaa
    22141 caagatatag atgaagtttt aaaaactgta gaagaacttg aagttcaaat agatgattta
    22201 agtaaaaatg atttaaagca aaaattcgtt actctaacag agaaagtaag aaaacttgaa
    22261 gatttattaa aagacgaagg tattatataa aagaaagctg agtttagaaa tttctaaact
    22321 cagctttttt taaatctctt tttcattttc tatactatct tcttttttaa actctttaat
    22381 tataaataat ccagttatta ttgtagacaa gaagtctgaa actgtacctg ctaaccaaac
    22441 tcctgttaaa cctaatcctg caatcttagg taagaccaat gttaaaggaa ttaaaagtat
    22501 aacctgtctt aataaactta aaaaagttgc taacttagct ttacctattg attggaaata
    22561 gttagatcct attattgata tacctattat aggcatcata aataaatata ttcttaatcc
    22621 tggaactcct atttctaaaa gcttagggtc tgaattaaac atacttatta ctaaggctgg
    22681 gaatgcttga attaatattc ctcctaaaac aagtattgtt gtcgctgcta tcatagctaa
    22741 cttaactgtt ttttgagctc tttcatactt cttagctcca tagttatatc caattatagg
    22801 ctgtgaacct tgatttatac caaatatagg cattaaaaat atcattgcta ttgaagaaat
    22861 tgccgccata gctcctatag ctaagtctcc tccatacatt cttagtgcat tgttagctat
    22921 tacttgaact acacttgcag ccatctgcat tgaaaatggg gctataccta tagccaaaac
    22981 tatcttaact aatctccaat ctaatttaag agtttcaaat tttaatttta agtttgatcc
    23041 tccaaatgta taataataaa gcattattaa tgtagtaact atttgagaaa ttactgtagc
    23101 taaagccgca ccttttatcc ctaagttaaa tacaaatata aaaactgcat ctaatattac
    23161 atttataata catccaagca ccattactgc agctgacatc tttggatttc catctgctct
    23221 aattgtacta tataaagaga atgacattaa attaaatata gttcctaaaa gtattacatt
    23281 tatatattcc tttgcataaa aaattgtagc ctcacttgct ccaaataagt gtagaatact
    23341 gtttgcaaag acaattccaa caatagttaa aactaaagat attatagtta ttaagaccac
    23401 agagtttcct aatattttct ctgcatcttt tctcttattt tgcccaaatt ttatagagat
    23461 gtttgcagta gcaccaatcc ctataagcat tccaaaacca agtaatatag acataattgg
    23521 catagttata ccaactccag taatggccat tggcccaact cctggaatgt gtccaataaa
    23581 tattctatca acaatattat aaagtgcatt tacaagcata cctataatgg ctggaaccga
    23641 atactttatc aaaagcttta attctgattc ttcccctaat aatttttgat ccttcataag
    23701 cctctcctcc tttaaatcta tatttcttaa atatatacta acttttccat tatacattaa
    23761 ttttaatttt ttagtcagtg aaattttaaa aataaaaaac tatataggaa atcaaacttt
    23821 ttttctccat aaatttaact atctatagat aaataaattt gattcttata tagtctttaa
    23881 aaatcttaaa ccgttacttc tttatatatt aaatttccac ctattaaggt aagagctgta
    23941 ataatattta aaactagtga aaatattatt attgatagga cactttgatt taaaaacatt
    24001 gcaaatgtca ttgtaaaaaa gttaattata aaaactaaag caaactgctt taaaagaata
    24061 tctaaaaaat tattttttac tagtaaaaaa ctttttttta tgctttctat tacatttatg
    24121 ttttcaaata tagaacattg catagctatt acaaaaaaga ttgatattac tattcctaaa
    24181 ggtgcaaata tgcttgagcc taaagaaaca gcaaagtttt gtataaggaa tactcctaga
    24241 ggaaataaag agtttttaaa ggatgatttt atagtctcaa tccaagatac cttttcacct
    24301 ctaataaaat tgttagctaa ctttattata gctattgatg agaatcctaa gctgaatata
    24361 ccaattacgg aaaatatagc atttcctctc cctataatag taggaagtgc aaaaagcaca
    24421 gatattattg ctatatcgcc taaattttta ataaatatgt taaatacaga ttttaaaaat
    24481 tgtccaactg ataatttttt attgatattt tgatccataa aaatcctcca atcaaaattt
    24541 atattaataa ttatcatatc aaaattaata taagttttgc ttgagttcat tttaatttaa
    24601 cactttctgt atatttggtt tacatatttt ctatactcca agataaaata ggtataatcc
    24661 aactaatatt ataattgttc ctaatatatt ttttaatact cttcctgtct ttttaccctt
    24721 ttcagagtag gctattttct caactaatcc aaaggatgtt ccagctaaaa ttattaaaac
    24781 actatgacct aatgaataca ttaaaagcat tatcattcct attactaagt taccactagc
    24841 agctataaag actattattg ctgccattat aggtgttgca caaggagatg ctaatattcc
    24901 acttaatatt cctaaaaaga agacatctaa aatcccctct cttcttttag tgattttaca
    24961 cgcatctttt ttatctccaa ataaatttat tacatcaaga acttgtagcc ctgatcctat
    25021 cataataaaa gccaatatta tataccaaaa cttccctgca cctaaaaagg cttttcctat
    25081 taaggctgta agtaagccta ttacagtaaa tgttattcca agtccaattg aaaatatcag
    25141 tgaatactta aatgcttttt tattatcctt aatctcacta ccttgaacat atcctattaa
    25201 taatggaata gatgaaaggg tacatggact aaaagaggct actactcctg caaagaatga
    25261 tagtattatg gctaaaaatg tattattaga tatgacacta ttaaaataag ctaaaaattc
    25321 catattctat ttaaccccca aagaatttaa aatggtttct atattattct ttctcataag
    25381 cccttgatga acatagaatt tattaccttc tttatctaaa aacacagtag ttggaacaat
    25441 attagcatca tatttatatg ctaaatctat atttttttcc tcttctaatc tagcatagta
    25501 aatattaaat tttccttctc tttcttttga aacataactt aactcttttt gcatttcgac
    25561 acaataagga caagtatctg ttttaaacat tattattgtt ggtatgccct cttttgcttc
    25621 ttctaaattt tcaaccacat taattgactg aaaatttatt tgctttattc cctcaatata
    25681 gtttttgcca atatatatac atattaatga gaaaaggatt aaacctaata ttattaattt
    25741 tctcttattt ttcacctccg actcctccct tactaaaatt taaatttttc cataaataaa
    25801 aaaaggtaac ataatttaat cttatgttac ctaatgatta tgcatttttt atgcattaat
    25861 tatttacagc tatctctttc tattaaatca tgatctaata cgaaatgatc ttgatctaaa
    25921 ggtttattat ttattatttt aattaacatt ctcatagcta ttgatcccat atcatacatt
    25981 ggttgtgata cagtagttaa ttttggatag aatacatgcg ctaaatctat gtcgttaaat
    26041 cctataacgc taactttttc aggaaccttt attcctttat ctcttaaagc gtttatagcc
    26101 cccatagcta tttcatctga agcacagatt ataccttcta ttttttgacc attttcaagc
    26161 attgctaaaa tagcatcata tccagtctta acctttaagt ttttaaagta agttaattct
    26221 tctttcattt ctattccagc atcttttaat gcagcttggt atcctttgta tcttttaccc
    26281 caagcattat ttaaatcacc ttctacacct atgaaagcta tgtttttcat acctttatca
    26341 attaaatgat tagtagattc atatgtagct ttttcattat ctatagttac acttgggaac
    26401 ttaccatttt gctcatcatt ttcaactaaa actgttttta aatctaattc atttattaaa
    26461 tttaaagttt cttctcctag tgatgaactc atgtaaagaa caccatcaac cattttttcc
    26521 ttaagaactc ttaagtattc tttttctttt tctaaatcta agtctgaatt acataacatt
    26581 acattatatt tatatatgtt agctacgtcc tcagcacctc taactatttc aggatatatt
    26641 tgatttgata tatcaggtac taatatacct acagttcttg ttctatgtgt ttttaaactt
    26701 cttgccacta tattaggtct atatcccagt ttttttatag cttctaaaac ttttttctta
    26761 gtttcttcat ttacaacatc tatgtcattt aatactcttg aaactgttgc tattgacact
    26821 ccagcttctc tagcaacgtc tttaattgaa gcagccatct aactcaactc ctatactcta
    26881 acttattacc tatctatttc ttaatacttc aatattataa tgataacttt acatttatac
    26941 aaggaattta tgtatatttc tgcaatttag tcaaattcct tttaattttt ctttaaaaaa
    27001 cgttttctta ttaataattg taataaaatt aaaatattaa ttcaatgcaa atattattat
    27061 atattataaa attttctaaa atgattgaat tatgttgtat aatcaaatat atcattactt
    27121 attgatatat taaccatacc tttaaaaatt tataataaat aaaagagaat ttctaggagg
    27181 aaactttaat gtataaagat atagatataa cagaatctat aaaattaata gatgaaaata
    27241 aagatttact aattttagat gtaagaactg aagaagagta taaggaagat ggatatatta
    27301 aaaatgccaa acttattcct ttaggaagat tactattctc tatagatgaa cttgatggat
    27361 atgaagattc accaatactt gtttactgta gaagaggttc tagaagtatg caagcttgtg
    27421 aaattttaga ggataatggc tttacagaac tttataatat gatgggcgga ttttcaaagt
    27481 tagttaatga aggaccaaaa gaactttgga ataaataaat tacataacaa aagagagcaa
    27541 taacttgctc tcttttgttt ctattttgtt ttaattaagc tctttttgaa acagccattt
    27601 ttaatatttc accatttact ttagcttctg agtaatcagc atctccgtta taaacaattt
    27661 cttctgttaa agtttctttc tttataacat cttcaaattt cttgattaca tctaaaagca
    27721 tatcattttc agctacgtaa agagttatct tatcagcaac ctcaaatcca ctttcttttc
    27781 tcatgttttg gatttttgat atgatttctc ttacatgacc ttcttctctt aattcatcag
    27841 ttatagtagt atctaaaaca acaccaactg atccttctcc agcaaatgcg aatccttcta
    27901 atccttgcat tgttacaagt aagttttcag catttaattc tatttcattt ccatccacag
    27961 tgataacttc tactccacca ttttggattt tttgagctaa ttccatttgg tttcttgaag
    28021 ctatttcttt tcttatagct ggtattaact taccataagc tcttcctaaa acaggtaagt
    28081 taggcttaat ttcgaagtta acatatttag aaaggtcagc tcctaattct acttccttga
    28141 tgtttaactc atctttaatt atgtctccat aataatctgg aagctctgaa gttgaaagaa
    28201 gcatttttga aagtggctgt ctattcttga tgttagcacc gtttctagca cttcttccaa
    28261 gtttaactat cttataagct aaatccattt tttcttctaa ttttttatct atagcctttt
    28321 catcaacttc tggccatgct cctaagtgga tactttctaa agcattttca tctaaattaa
    28381 ctactaagtt ttgatagatt tcttctgtta taaatggtac gaatggagct gcaactttac
    28441 ataaagttac taaaactctg tataaagtag tgtatgcacc tatcttgtca tcagttaatt
    28501 cttctgacca gtatctagat ctatttcttc ttacatacca gtttgataat tcatcagtaa
    28561 actcttctat ttctaaagca gcttgagtta ttctataatt atttaaatga tcatctacat
    28621 ccttaactaa agtattaagt tttgacatta tccatttatc cattacgtta tctgatacga
    28681 aatctttgta gtctaatgga ttaaagttgt ctaactctgc atataaaaca tagaatgagt
    28741 aaacattcca taatgtgctt aagaattttc tttgagtttc agcaacatct tctattgaga
    28801 atctagttgg taaccatgga gcacttgcag tatagaaatg ccatcttgta gcatcagcac
    28861 cttgtgaatc aagtacttca aatggatcaa ctacgttacc cttagatttt gacattttta
    28921 atcccttctt atcaagaacg tgacctaaaa ctatacagtt ttcaaatgga tttctatcaa
    28981 atatagcagt tgatattgct aataatgtat agaaccatcc tcttgtttgg tcaacagctt
    29041 ctgatatgaa ttgagctggg tagttagctt caaataattc tttgttctca aatggatagt
    29101 gatgttgagc aaatggcatt gatcctgaat caaaccaaca gtctataacc tcagctgttc
    29161 tcttcatctc tttattacaa tgaggacaat ttaaatgaac tttatcgatg tatggtttat
    29221 gaagttctat atcttctgga acatttattc ctttagtttt taactcttca atgcttccta
    29281 tacattctct gtgtccacat tcacattccc atattggaag tggagttccc cagtatctat
    29341 ctcttgagat gccccaatct ataacattct ctaagaactt accaaatcta ccgttcttga
    29401 tgttgtctgg gtaccaattt attttttcat tgttagctaa aagtttgtct cttaaagttg
    29461 tcattgcaac aaaccagctt tcttttggat agtaaagaag tggtgtgtca catctccaac
    29521 aatgtggata tgagtgagta tgcttttcag ctttaaataa tttattttgt tcttttaagt
    29581 aatcaacgat ttgttggtca catttcttaa cgaattttcc agcccatgga gttacttctt
    29641 ctacaaagtt accgcttaag tcaactaagt ttaagaatgc taatccattt tgctttccta
    29701 ttaagttatc gtcctcacca taagcaggag ctatatgaac tataccagta ccatctgata
    29761 aagttacata gtcaccatga acaactacga aagcttttcc ttcagttgga gttacaaatg
    29821 gtaataattg ctcatattcc attcctaata actcttcacc tttgaactct ctaactattt
    29881 caaaaccttc tcctaaaacc ttaggtgcta attctttagc taagatgaag atttcatctc
    29941 cctcagcttg cttagcttct acatagtcat aagctttatt tatacataaa gcaacgtttg
    30001 aaggtaaagt ccaaggagtt gttgtccaag ctaagaagta cttattatct tctcctttaa
    30061 ctttaaactt agctacagca gttaagtcct taacatcctt gtatccttgt gaaacttcgt
    30121 gtgatgataa acttgttcca catcttgggc aataaggcat tgttttataa cctttgtata
    30181 aaagtttttt atcccacatt tgttttaaag cccaccatac tgattctatg tatggattgt
    30241 ggtaagtaac atatggattc tccatatcta cccaataacc tatttgctca gtcatttttt
    30301 cccacatgct aacatatgtg aatacactat ctttacattc agttacgaat ttttcaacac
    30361 cgtaatcctc tatttgaggt ttacctgaaa ttcctaactt cttctctatt tcaagctcaa
    30421 ctggaagtcc atgagtatcc catccagctt ttcttataac tttatatccc ttcataactt
    30481 tatatcttgg gattatatct ttcataactc ttgttaatac gtgacctacg tgtggtttac
    30541 cattagctgt tggaggtccg tcatagaaag taaagtattc accatcttga ttcatgtcaa
    30601 agttcttttt tattatgttt ttttctttcc aaaggtttgc aacttctttt tctacgccta
    30661 caaaaccctt aggtaattca acttttttat acatattgaa actcctttcc ttctcttgtt
    30721 taatatttta attatttact tataattgat tatttagcca gacttcttta agtaaaataa
    30781 aaaaaactcc atcctaaaag gacgaagtta tattcgctat accacctaaa gttcaaacct
    30841 atctatataa ttcaattata aatattaaaa ttttacgttt ttatgatagg taattaaaag
    30901 cacctataat gctcgcatcc tgtaacgtgg atatacggga taacttaata agaatatatt
    30961 cctttcagtc tccaactccc aggtgatttt ccttaaatat acctatagtt tcacaccaaa
    31021 caactactct ctgaataagg ttctatataa gtacttgccc tgttcatagt ctttaaagaa
    31081 attatataat agcactttta aaaagtcaac gaaacaaatc catatatttg aaagctttca
    31141 ttaattattt aatttgcttt tataataaaa attctatttt tgtacaacct aatattataa
    31201 gttaatacct atatattaaa taacaaaaat taatcaataa tttttattat ttaatgttga
    31261 cttttcttat cagttaactt ataattaaat catcaaatac attccattcc cttttgttaa
    31321 tcgtgctgtt ttactttgat gtaattagga ggaaaatagt atgaatgata acaataattt
    31381 aaatcaacaa gtaattgata aaatgactaa agtttgtatc tgcagagcaa tctcaagagc
    31441 aaagataaag gaagctatca aaaatggtgc acacacagtg gaagcagtag ctgaaaaaac
    31501 tggtgcaact aaaggttctt gcaaaggttg cagatgtaga gacaaaattg aagaattaat
    31561 tgaagacaac caataattct tttaagcaaa acttatttac tgttttaatt ttgtatctcc
    31621 ctaaaagagt accctcgggt actctttttt tacttttaca taatatttat tttgtattaa
    31681 aaattaaaaa ataaaagagc ctgtatcaaa tttaaaatta ttttgataca ggctcttttt
    31741 atttaaattt caaattcatt taattgtaaa tttttagacc aactactata gtttctagga
    31801 tatccttgat ttgtaagtgt tcctacataa taaaactttt ttaaatcaaa gcagttattt
    31861 ttaacaactt taaatccttt taaaaggtaa tgtaatctac tttcatacac ctttccaata
    31921 tctttagacg ttggctctcc ccaagcaaat attatgtgag aactatttat taggctttca
    31981 aatattatta agtcattttt taagtattct ccattctcaa taaatccatc atttccacat
    32041 aggaatagct cgcctttggt attataaact tcttctagca catccttgct atattcaata
    32101 acaggaaata ggaatacaaa acttatttct tttattattc caagttcatc ctcattattc
    32161 attaaatatt ttatagcctt atttaattct ttttttaaat cttcagcatt atgtgatttt
    32221 ggagctctag taataactaa tatattactt tccccttctt cattaatagt aaaagtaacc
    32281 ttaaagcctc ttccactttc atgatctaat aaaatattta tattattttg atttttacaa
    32341 tacttttgca taagcgctcc cctttttacc aactctcgtt ccttagactc aatatattag
    32401 tattaagtat attctaactt tagtttagta tataatcgaa tttttaaaaa ttctacaaat
    32461 ttatataaat ttaatttatt cacttaaata atatcttatc taagaaagta aattttattt
    32521 attctttttg ttttcttcta tttgtgctgc taactcatta agatattccc atctctcata
    32581 tttaaatgct aattcttctt caacatttgc cttttcttct atcagttctt gaagtctacc
    32641 atactctgat gcatgttttg ccattaaaga atctaattct tctatttttt cttcaagggt
    32701 cattatttct tcatctatag tttcaaattc tctttgttct ttaaatgaaa attttaatac
    32761 tttacccttg tttttatttt tatccacaga tgactctttt ttatttttat ccacagccac
    32821 ttctttttct ttattgttaa cagcctcttc ttctttgctt agaagataat ccccatagtt
    32881 tccagtataa atatctattt ttccatttcc ctcataagaa aatatcttgt tacaaactct
    32941 atctaggaaa tatctatcat gggatacagt tatgacaact ccaccaaaat cgtctaaata
    33001 atcttctaat ctatttaaag tttcaatatc taaatcattt gtaggctcat ctagtaaaag
    33061 cacattaggg gcttccatta gaactcttaa aaggtgaagt cttcttcttt ctcctccaga
    33121 taacatacct atcattgtcc actgaagtgt tccatcaaat aaaaactttt cacacatttg
    33181 tgatgcagtt attctttcac ctgtggcagt ttgtaagtac tctcctgctt cttttatgta
    33241 atctatagct ctcatctctg gatgcatatg tgtatcttcc tgtgagaagc aagctatttt
    33301 tacagtttca ccttttatta tttctccact atctggctta acttttcctt ctatcattcc
    33361 cataagtgtt gatttaccaa ttccattagg tcctacaata cctattctat cactttttag
    33421 tactatatag ttaaaatcat caacaatctt tttatcacca aatcttttat ttaggtgttc
    33481 tatctctatt attttctttc ctaatctcgt tcccataaca gagatttcta tattttcatt
    33541 ttcaataaat ccttcttgat ttactaattc atcaaatctt tgaagtctag ctttttgctt
    33601 agtagttcta gcctttgcac ctcttcttac ccaagctagt tcttttctca taagattttg
    33661 tcttttttcc tcactggctt tttctatggc tattctctcc atcttctttt ctaagaaaac
    33721 agaatagttt ccatcatagc taaataatct acctctatct aattctaata ttctgtttgt
    33781 tactctatct aggaagtatc tatcatgagt aatcattaaa agagcgcctt ttctactatt
    33841 taaatattct tctaaccaac ttatagtttc attatctaaa tggttagtag gctcatctag
    33901 tattaataat tcacaaggag ttattaatgc agctgcaagc attattctct tcttttgtcc
    33961 tccagaaaga tttcctactt tttcttcaaa attagttatt ccaagcttag ttaaaacatt
    34021 ctttgcttca ctttccatat cccaaagatt taaagcatct atttttcctt gaagctttat
    34081 taatctttca gaatcattct ctttaacttc gcccttatct attttttcaa gtaactcttc
    34141 gtactctctt aatattctca tttcctctga gttaccctta aaaacttgct ctaaaacagt
    34201 agcattatca tcataatctg gtgtttgagc aagatactct attcttactc tatttccctt
    34261 tgttactgtt ccttcgtctg gttcttcaac tccagcaact atttttaaga aagaagattt
    34321 tccagttccg ttaactccta taagaccaat cttctctcct tcatttattc ctaaagatac
    34381 attattagct aaaatttttt ctgaatagct cttacttata ttttcaagag ttattaaatt
    34441 catcgattat ccatcctttt caaaataaat cttattttca ttataatata aactcaagtt
    34501 tcttccaaaa ctaaaataag agctgcatca aaatccattt aagttaagta ttgatacaac
    34561 cctatctatt taaaataaat ctaaaaatta aacttattat tcatattata catccaaaaa
    34621 tataactttg aataagagat aattatattt taaaacctca tataaaaatt attccaataa
    34681 tctctatttt gcatcatcat ttctctaagc tcttcgtcat taccatactc atcataccac
    34741 ttttctaaaa tttcataggc aatgccatga tataataaac ttcctataac aaagggttta
    34801 gctggcacat cacttaaaga cattatggtt acacctgttc ctatggttcc atatataata
    34861 gctctctctg gagtaacaaa agatttcgcc ttctttttaa agtcagtatc tcttatgtat
    34921 ttttttaatc ttttaggtgt aggaataact atagctttta tcatttttca atccttaaaa
    34981 ttactcttat tagaaatata tattaaatat tatcttatta ttctaaagta ccaatttatt
    35041 tttcttaaat gcattttaaa cctattggaa agcctagaat atgttataat cttataagaa
    35101 ttattttgga aggagctatt taaatggcga gtaagtttta tttacacaaa ggtaaaaaca
    35161 aaaaagctga acaaggcaga ccttggatat atattgatga aataaacgaa tatgatggag
    35221 attatgaaaa cggagatata gttgaagttt acaatcataa aggttatttc ttaggaaaag
    35281 gttatataaa tgacagaagt aagataacta taagaataat gactaaagat atagatgaag
    35341 aaatagatga ggatttcttc aagagaagat ttaaaactgc atgggaatat agaaagaaag
    35401 ttatagatac atcttcatgc agattcatct ttggagaggc tgatttcctt cccggtttaa
    35461 cagttgataa atttgaagat tattatgtaa ttcaaatatc aactcttgga atggataaat
    35521 atagagacct aatagttaaa attctagttg aggaatacgg tgctaaaggt gtctatgaaa
    35581 gaagtgatat aaaaactaga gaaatagaag gtttagagca aagaaaaggc ttcttaactg
    35641 aaccatttga tacagatata caaatagttg aaaatggagt taaatacata gttgacttag
    35701 aaaatggtca aaaaactggt ttcttcttag atcaaaaaga aaacagagct gcaatgcata
    35761 gaatatgtaa agatatggat gttttagatt gcttcactca tactggctct tttgctttaa
    35821 atgctggtat agcaggtgct aaatcagttt taggaataga tgtatctcaa cacgctgtag
    35881 actgtgctac tagaaatgct gaacttaata atcttcaaga tagggttaaa tttgaaaagc
    35941 ataatgcctt tgatgtatta ggagattggt caagagaagg aaaacaattt gatgttgtta
    36001 ttttagatcc accagctttc acaaaatcaa gaaatactgt taagcaagca ataagaggat
    36061 ataaagaaat aaatcttaga ggaataaaaa tggttaaaga aggtggttac ttcgctacat
    36121 gctcttgttc acattatatg gatgaagaac aattaaagaa aactgtagct gaggctgctc
    36181 atgatgcaag aagaacttta agacaaatag aagttagaac tcaaagtgca gaccacccta
    36241 tactttggaa ctctgacgaa tcatattatt taaaattctt catattccaa gtattctaaa
    36301 aaaattttaa atcttaagta taaaaaaaaa tatattggtt aatatactaa tgtagcaaaa
    36361 atatataaag ttaatatctt aacttataaa taagaggtgc cttacaaaat tggcatacac
    36421 tctaaaattt gtatttaagg gttaatcatt aatttaaata ttaaatatat tattactgtg
    36481 aggtgcttta cataaatggc atacactcgc taaatggtca taatcaattt aaatatttaa
    36541 atatctattt ttagctactc actagttagg actgtttatt ctatcctaat taatgtacat
    36601 ataatattag atgtattaaa agagaaaggt atctgatttt ttcagatacc tttcttaatt
    36661 tatttatgaa actgtttttg cagaaactat actaacccct gttcctaaaa catcttctat
    36721 aattacatct ccaactttaa ttggaagttt aacagaagcc ttgttaattt ccttcattac
    36781 atcaaatatc attccctttg gaataggttc ctctgtttta actggaacaa ctatgttgtc
    36841 acaaccatct attttaacag ttgatgttac tattctagtt ggatttttaa cctcgtttaa
    36901 tccataaatt tctcctcttt tacatgtgtt tcctgtaacc ttttctaact ctgtgtctac
    36961 tgttaaatga catcctctag gacatactat gcatattaat tcttttacca ttattattca
    37021 ccatcctcta atgaaattgt tattgctcct ttagcatttt ttagcttatc tttaagaatt
    37081 gtaattctct ccatttctgc aggaacaaca tgaggtttct taaagctttg aataacattt
    37141 ccttcttcat ctctaactac tatttgttta tttttaaata catttctaac tctcataaat
    37201 acatttaaat ttccatctaa agattcaatt tcaaattttt gaggaactgt ataacttata
    37261 ttttctccat tttttatttc agtgaaatga tttaatctct ttttaccttt aatatattta
    37321 gcagcattct ttcctgcatt tttagcttcc tcagttacaa agtcaactaa gtcatgaaca
    37381 tggacaacat taccacaggc aaatatacct tctctatttg tttccatact ttcagaaact
    37441 acaagtccat tagttctaag gtctaattca actcctgcat tttttgaaag ttcattttct
    37501 ggtattaatc caactgataa taataatgta tcagcatcaa attctatttc agtacctttt
    37561 ataggttgtc tattttcatc aaccttagct ataataacct tttctaatct tccatcacct
    37621 ttaacatcaa caactgtatg agataggtat agtggaattc cataatcctc taaacattga
    37681 acaatatttc tagttaatcc atttgaataa ggcataagtt caacaacagc ctctacctta
    37741 gctccttcaa gagtcattct tctagccatt ataagaccaa tatcccctga ccctaagata
    37801 actacttttt tacctaccat gtagccttcc atatttataa atctttgagc agcccctgca
    37861 gtgaatattc ctgatgttct atctcctgga atacttatgg ctcctcttgt tctttcacga
    37921 catcccatag caagtactat agcttctgct tctattgtta aatatccttt ttcagtattt
    37981 atagcatgta ttatttttcc ttctatttct aaaaccattg tatctaacat aacttctatg
    38041 ttagttcctt ctaccatgtc aataaatctt ccagcatact caggtcctgt aagttcttct
    38101 ttaaaggtat gaagaccaaa tccgttatga atacattgat ttaagattcc acctagttct
    38161 ttatctcttt ctataattaa tattttttct actccatttt catgagcttc ataagctgct
    38221 gctaatcctg ctgggcctcc acctacaact actaaatcat atttcataac taaatcctcc
    38281 tactttgttt ttccacttat tatatatgaa ccatttttat ctaaaagcac ttcttctata
    38341 ttcataccag tttcacgaga tattatttct tgaactctag gtccacagaa accaccttga
    38401 catcttccac aaccaggacg gcatcttctc ttaactccat caatagttct agctcctaaa
    38461 gttctattta tactatcaac aatctctcct tcagttatat tttcacatct acaaattatt
    38521 cttccatatc tactatcttt tttaattaaa tttgcttttt cttctggaga aagttctata
    38581 aagtgtactt gttctctttt ttcattgaaa ttatcttttt tctctaaagc tagtccactt
    38641 tcaccaagca ttttaactac atccactgca atagctggtg ctgaacttaa acctggtgat
    38701 tttattcctg ctacatcaaa gaatcctttt acatctttag cttctccgat tatgaagtca
    38761 ggcttatcag ctatagcacg aagtcctgca aagtttctaa tattttctct aaaattaact
    38821 ttgttagttg tttttaaaga aacttctcta accttagcta atccataata ctctgtagaa
    38881 actgaatctt tatcacaagt atcaatagca tctggaccaa ctaataaatt tccatgaact
    38941 gttggagtaa ctaaaacccc ttttccaagt ttagttggac attggaatat tgtcttttca
    39001 aataatcttc cttgactctt atctaaaaca tagtattcac cttttctagg agttatctta
    39061 aagtcttcct cacaaaccat attgtgaatc ttatcagcat aaatacctgc tgcgtttact
    39121 ataaatctag cttctactac atccccattt tcaagtttta ttttaaagtt ttcatctttt
    39181 tcaataccaa caactttaga gtttaatctt aattctcctc cattgtctat agcattttct
    39241 gaaagagcta tagtaaattc atatgctcca acaattcctc ctgttgggca gaataaagct
    39301 ccctttacat ctttatttaa gtttggttcc atttctaaaa cttcttctgc tgataaaagt
    39361 tttactcctt ctactccgtt ttgaattcct ctattatata aaacctctaa ttctttttgg
    39421 tcatcttcat caaaacctat aattaatgat ccatttctct taaaaggaac tgataagtct
    39481 ttacaaagct tatcaaaaat cttatttcct tcaacattgt attttgccat taatgttcca
    39541 ggttcaggat catatcctga atggacaatt gctgaatttg ccttagatgc tcccatagca
    39601 acgtcatttt ctttttctaa ttctacaacc tttaaattat actttgttaa ttctctaaat
    39661 attgctccac ctattactcc agatcctata attgctacat cataaatcat taaatatcgc
    39721 tccctttttt aaaaataaaa aagaaccaaa ctcaaaaaag atataagaat atcctttttg
    39781 tgtctagttc tcttctctct tacacacaaa atttaaatta tttattaact ttatttaatt
    39841 atatcacaat tgtgtaaatg tttccagtat tttttttaat cttcaattgc ccattctttt
    39901 gatctttcaa tagctttttt ccatttctta taatatttat ttcttctctc ttctgaaata
    39961 tttggttcaa actcttcact agcaaaccat aacttagcta tttcttcttt atcactccag
    40021 tatccaactg ctagcccagc aaggtaagct gcccctaaag ctgttgtttc tgtaattata
    40081 ggcttaatta cctttttacc aactaagtct gattgaaatt gcattaagaa attatttcta
    40141 cttgctcctc catctacctt tatacaattt atatcatatc caacatcgtt tatcatagct
    40201 tcaagcacat ctcttgtttg atatgcaatt gattcaagtg atgctcttat tatatgattt
    40261 ctattagttc cacgtgttaa tcctactatt gttccacgag catacatatc ccaataagga
    40321 gctcctaatc caacgaaggc aggaacaaca tatactcctc cattatcttc tacttttgta
    40381 gcaaagtatt cagtatcact agaatcactt ataattctta attcatctct aagccattga
    40441 ataacagctc ctccaacaaa tactgatcct tctaaagcat attgaacttt tccgtgtaat
    40501 ccgatagcta ttgttgttaa aagtcctgat ttacttttaa caagttcttc tcctgtgttc
    40561 attaataaaa aacatcctgt tccataagta ttttttacat cacctttatt aaatgcagct
    40621 tgtccaaata aagcagcttg ttggtcccca gctattccag ctataggaac tcttattcca
    40681 ccttttgctc caaggtttgc atatccataa atttcagatg aattttttac ttctggtaac
    40741 atacttctag gaatgtctag ttcctttaac attctttcat cccattcaag gttctttatg
    40801 ttaaatatca tagttctaga tgcattagta taatcagtta catgaacttt tccatttgta
    40861 agcttccaaa caagccaact gtctactgta ccaaataaaa gctcaccctt ttcagctttt
    40921 tctcttgcac cttcaacatt gtctaatatc cattttattt ttgttcctga aaaatatgcg
    40981 tcaagtacaa gtcctgtatt ttctctaata tagtctacaa gcccatctct ttcttttaaa
    41041 tcatcgcata tatcagcagt tcttctacat tgccaaacaa ttgcgttgta tattggaacc
    41101 cctgtatttt tgtcccaaac tattgtagtt tctctttgat tagttattcc aatagctgct
    41161 atattttctt gagttatatt acacttagcc ataacttctt gaagtacact atattgagtt
    41221 gcccatattt ccattggatc atgctcaacc catccttctc taggataaat ttgattaaat
    41281 tccttttggc ttactccaat aatgttttgt tctttatcaa aaattattgc tctagaactt
    41341 gttgttcctt gatctaatgc tactatatat tttttcataa aaactcacct cttaatttta
    41401 tatcttccat atatcctctt ttgttgttga tatgcatgta gctcctgatt ttaaagcttc
    41461 catcacatcc tctttatctg aaattaaacc acctactatt aatggtttat gtattgtttt
    41521 tgataattct ttaataactt taaataataa tcctggtaac atttctacag catcacattc
    41581 atccactaag tgattttttg tgctttgaaa agaaagacta tctaaaatga aaactctctg
    41641 aatagctact aaccctaatt tttttgcact ttttactgtt tgagccttag tgcttattat
    41701 tccatcaaac ttagttttct ctttcaaata ttctaaaacg atttctctat ttgtaagtcc
    41761 atcaactaaa tctatgtgca atattccaat cttatttttt tctgatattt tatgtgaaat
    41821 ttctataata tccatgatat taccaaacag aacaaaaatt acatcaacat cagcttctaa
    41881 tgccttgttt aattgctcca tatcctttac agctgccaca atagggttgt cctcaagtat
    41941 tcttttaaaa ttaatcattt tatatagcct ccttaaaatt gtccaaaatt aatatatctt
    42001 taaaatatat ttgtatatac tattgctcct gctatagcac caagtattgg tcctacaatt
    42061 ggaatccaag cgtaagccca atctgagcct cccttatttt tcataggaag caagaaatgt
    42121 gcaagtctag gtgagaaatc tctagctgga ttaattgcat atccagttgg tccgcctaaa
    42181 gatactccta taactaaaat taataatcca acggctattg gcataattcc atctaccatt
    42241 tttgcatcac caaaccctaa gataccaaac attaaaacaa aagtacctat aaattcagtg
    42301 aaaaaattcc atttgtaatt tctaatagct ggtatagtac aaaatgtacc aagctttgca
    42361 tcatgatctt cagtagattc aaaatgagta taataagtta aataaactag tacagcacct
    42421 atgaaagcac ctataaattg tgctatacaa aatactacaa cttgattcca agctagtcct
    42481 cccgtaattc ctaaagcaat agttacagct ggattaaata ccgctccact tactctagaa
    42541 aaaatataaa caggaatagc aacagctatt gcccatccta cagtgactac aataagatca
    42601 gcgctattac ccttagtttt tttaagagct atatttgcaa ctacaccgtc tcctagtaat
    42661 atcaaaagcg cagttccaaa aagctcagcc aagaattgat ttaccatgac tatccaccac
    42721 ctagtaatta atatatttaa ttttaaaaaa gcttaaaata aaaagagtta aacccaaaaa
    42781 ttactttctc gtgtttaact ctaaatactc ttacacaagt tgaatatata cgtatatatt
    42841 tttattttta caatatttat tttagcacct attgaaattg ttttcaataa attaaaattt
    42901 taacaatcta tccataaaat taaaaaatat ggtattatta taatttattg tttttctaat
    42961 gattaattaa aaataattat tattaattaa tcattagaat tttatattta aataaattgg
    43021 ttatatttca caagaattaa taactactaa tattttttct tcacaaaata tattactaca
    43081 tagagatact atatattcct aaatatattg aaaaatattt tattttaatt gtttttcaat
    43141 aattttaaca aaaaatatta atattaaaac ttgtttgttt ttaataaaaa tacttctcaa
    43201 aactttatta tatttttact ttaattcctg attctacagt ttttatttca ccatttttaa
    43261 attcaaaaac taaatctatt aaattctttg tagaacaata ctttaaatcc tcttctagcc
    43321 ctaaattttt aagaatatta taatgtttag catcttttat aaattcttct attccttcac
    43381 tactttcata aacatattta gctgttttag ctatatcagt aagttcaacc tctttatttt
    43441 tacatagttc acttattata taacctgcac acataaaatc atcacttgaa aattcaccgt
    43501 ttgttccaga attaacaaag actatttctt tttcttcatt ttctaaatat ttagctacag
    43561 cttgtccatt taacacagag gcaactataa ctttatcagc cttactacat aaatttaaag
    43621 ctctagttcc atttgtagta gacattatta cattttttcc ttctactatt tctcttttgt
    43681 attctaatgg agaattagag aaatcaaacc catctatttt taaagccttt ctctctcctc
    43741 caagtaaaac ttcttcacct aaagatttta attcttttgc tttttcaaaa gcctcttcaa
    43801 cactaactac tgggataacc tttttagcac cattataaag tgctgtatta attacagatg
    43861 tagcccttag catatctata accacagcta tctttccttt aagtacttca ctatctatgt
    43921 aatctgcaga aattataaca tcaattttca taaaaattcg ccaccttatt tttaaaatta
    43981 ttcttcaaaa ttctttcctt gataaatatc attatcttca agagccttat caatagcatt
    44041 taaaacttcc cattttttta aactccacat aggtcctata agaagttgtc taggagcatc
    44101 tcctgttagt ctatgtataa ccatattttg aggtattcta cttatggccg tacaaattaa
    44161 atcaacatat tcctcttgag ttaagaattt taatctacct tcctcataat ccttaaccat
    44221 tcttgttcct tccattagat gaagcaagtg taattttatt ccttgtgctc cacatctaga
    44281 aacataatca acagtttcta acatttgctc tttactttct ccaggaagtc caaatataca
    44341 gtgaactaca gtatctatcc ctctactttt taatccttcc atagccttat caaatacttc
    44401 taagtcatat cctctattaa aacttctagc agtatcatca tggattgttt gaagtcctaa
    44461 ctcaacccaa gtatatacct ttttattcat ttcctctaat aaatctaaaa cttcttcatt
    44521 tatacaatct ggtcttgtac ctatagcaag agcaactaca ccttctttat taattgcttc
    44581 ctcatatttt cttcttagtt cttcaacagg agcatatgta ttagtgtaag cttggaaata
    44641 tgctatatat tttccctctt tccacttctt attcatcatc tctttaacat tatcaaactg
    44701 agtgccaata cttaaagttc tttgtccagc ataatctcct gacccctttg aactacaaaa
    44761 agtacatcct cccttagcaa ccttaccatc tctattagga catgtaaaac caccatctaa
    44821 acttatttta aatacttttt ctccaaactt ttctcttaaa aatctattta aagtattata
    44881 ccttttgtct ccccatgttt ttcttttttc catttatatc accttttcat taaaatcaga
    44941 atttctttaa aataaaaaag ttaatcataa atttaagggt tatgattaac ttattttata
    45001 ttatactata gtttttcaag tttaaaatct tttagagata ttttatatct tccaattaat
    45061 ccactttcag tattatttga ttctattatt ggagtaacat tcataattat attatcatcc
    45121 tgtattcttt cataaataag attatatttc ttttctgcaa atatatcatc taattctaat
    45181 gaaactatac ttccaccact ttgataaacc ttaaatctag taccatttcc tttaacataa
    45241 tttacaacaa catatccatc atcatctgaa aaatttaagc tagaaatttt acatcccttt
    45301 aatacatcta atgatgttat ctttttccct aaagttttat catcattctt ttcatcttcc
    45361 tttccccctt catcttcccc ttctttagct gcagtctcct gtgcatcctc aacatcaacc
    45421 actcctatat aagaatcttc acctttatat gttgaaattg ctactttatc tcctgaaaat
    45481 gaaatttcta cggctgaaaa agcttcttta ggtacatcaa cctttgctac atctataaca
    45541 ttaacctttg gataaatttt atctggtatg ctttttaaat ctattaatgt attaatctca
    45601 acatcatcat cttttctaat ctttaattta tccttatcta aaaaaacatt ataaagctca
    45661 gttgatttaa gctctgatac cttatattct ccatcttcag tttttctaac ctctaaataa
    45721 tattgctcta aataaccctt tggctctcct tttttagttc tagttacata gaatttaaac
    45781 atagttttat ttcctaattg tgctgctcca acttgcttaa tacctgttat ttcaagctcc
    45841 ttactaggac taattattat atttttatca acctctttat cagataacct tttaatcttt
    45901 tctaaatcat tgcttttaat agcctccata tattcctctg caattgatat tgctatctta
    45961 ttattaaaat atatgctttc aacactttcc tttagctcct tattatcatt gaccttatta
    46021 cttattctat tacagccaat gaaaagaaat aaagagccaa taattaaaga tgccataatc
    46081 tttttaacca tttataatca ttcctccctt agaataagtc ctattttagg ttttccaaat
    46141 agtttaaaag tattaataaa ataataaata tactaaactt aatttttact tttttatttt
    46201 aagaataaac ttcctttaca acttatatat ttagttaggg aggtgattta atgaaaagta
    46261 ttataaaagt ttttcaagta gcttttgttt ttataggaac tatagtagga gctggacttg
    46321 cctctggaaa ggaaataaca aatttcttta cagtgtatgg atataaaagc ttttttacta
    46381 ttataatgac tggtttattt tatatttttc tttgtaacat aatttcaaga ataagcatta
    46441 aacatagttt aaattcctat agcgaagtca taaatacagt tagtcctaat attttaggaa
    46501 aactcactgg aatcataaca accttttact taatatctag tgcgtcaata atactagctg
    46561 gtagcggagc cttgttgaat caatactttg gaattcctaa tattataggt tcgatattta
    46621 tggctattat tgcttcgctt attcttatga gagaaactga tggattagta gagataaatt
    46681 ctatcattgt tccatcctta ataatagtaa caactacgct tatgattctt tatgttacat
    46741 ttgctaatga cccaattact ataggttctc ttgtttcttc tactccaaaa ctttctaagg
    46801 gagtttttac ttctggaata ctttattgtg gttataacat tctttgctgc tgcggggtta
    46861 ttgttccttt aagtaacgaa ataaaaagta aaaaaatcat gacctgggga attatattag
    46921 gttcagttct tctaacaata atatgtattt ttattaattc aatgcttatg gttaatcaac
    46981 cacaaatatt ccaatacgaa attcctctac tttatattgc tgatagattt ggaaagccta
    47041 ttcaaataat gcttttaata ataatattat ctgaaatgtt ttctacagag gtttctgatg
    47101 tatattctat aagtaaaact cttactaaaa tctttaaaat aaaatataaa catgccatat
    47161 ttgcagtttt aatagtagca cttccaattt cattaatagg attttcaaac ttaatatcca
    47221 cattataccc aatgtttgga gttttaagct tagtatttat agctcaatgt atttacttct
    47281 acttttttaa attaaaaaaa taaagataaa aagtaaaagc tgagcaattt atataccttg
    47341 ctcagcttta atatttaata aattatcttt ggtacatttt cacgtctatc cattattgtt
    47401 ggtggataaa atatcttctt ctcaacactt gatctaagtt ctgctatttt tgttactgca
    47461 acatagacat ctgatatttt ataccatgtt gcataatcta cctctcctgt ttgaggaagg
    47521 tcaaatattt tttgaaatgt ttttacagct tctctagttt taggaccata ttttccatca
    47581 acggcaatct taggtattaa aggataagct cttgaaatag catttaattg ttcttgtata
    47641 actcttacag gctctcctga atatccaaat tttaatgtat aacctggata tgatcttgga
    47701 cttcccttaa cttttttagc ggactttaat tctaaatcat caccataaaa ataggttaaa
    47761 atatcataag gaacctttcc ttcatctcct aagtatttac ttccccattg agtcatccaa
    47821 cctggacatt gtacttttat tccatcacaa tactgtgcta aaagaggctg cttagaattg
    47881 attcttttca tatatgttga gaatatttca tctacaattc tacttatgtt ctcataaaag
    47941 tttcttccat aactaaaagc atgatcaaaa gccgttgaac tagttatatc aaaattttta
    48001 ccttttcctc tataccattc agtatatatc ctgtttaaag taaatgatgt tatagcataa
    48061 acattagctc ttatagtagt ttcaggccac gttgaaaaaa tttcacaaca tgcaacattt
    48121 tttatataat ctttataatt aaccttataa ttaggtgctg tattatcatt tggtctcccg
    48181 tcatgaacaa ctatatactc tggaattaca ggttctggca atactgctat tccctttggt
    48241 agtggaagtt ccttttcctc tggttcaggt atcttcggag gaaaatttcc aactaatctg
    48301 ttaggctgta tatttataat cttttcttgt ctataatatc ccctagtctc aggtaaatta
    48361 atattttgaa ttgccactct tgcaggatag atttgtactc catttactgc tacaggcaaa
    48421 aaaccttctt tctcagcaat aacatttgca aaactataag gaattgtatt tggtttatta
    48481 gaattttcta ttggtggtgc atctaattca ataatatcag tacttcctgt attatttgta
    48541 tataatacta tagtatcccc tatagctata ccatcttgtc cagttggtgt tattttaacc
    48601 ttacatttat caataggtat atatctcctt tgttcgctaa aacattgaac cttcaatcta
    48661 cctaatgtca tatatttctc ctaataataa attaatacct tatagtttat gatataaact
    48721 tgtattttgt taataagtat tcataagtaa aatataatat ttttacttct aatgtctaaa
    48781 tcttttaatt ttatattatt aaaatattta atatatctaa tattataatt tcttaccata
    48841 tataactatc atctattagt taataattcc attgttctct ctaaacatac aaacccatat
    48901 cctaagtctg gatttggata agcctctcca gatatggtcc tccttgctcc tttaattaaa
    48961 taatatttaa tcttttgtcc atataaaaaa ggatcattgt ttcttataat accccattcc
    49021 aaaagaagag cgcatatccc agaaacttgt ggtgcagcca ttgatgtacc acttttagta
    49081 tcaaatcctt gccctggaac agtagataat atattttcac caggagcaac taaatctggt
    49141 tttattaacc attcaggtct aacaacgccc ctaccagaaa atgctgataa attattattt
    49201 aaaaaattat aacttccaac agagataacc ccttgtacag ttgcagggat tcctaatgta
    49261 ttataaacag atggttgtaa aaatcttgtt ttttcattta atccttctgc tataggtaac
    49321 caaatatcaa aatatccatc ataattattt aattttctaa ctataattct ccatccacca
    49381 gaagttattg tgtctcctaa tggtaaaata cttattgtag tttgtccttg tatatcaaaa
    49441 ggtttaggac cagtactata tacaactatt ttttcttttc caacaaatct ttctctataa
    49501 gattcagata attccatagg tcccgtactt actccagttg gtgaaattac ctcaactgat
    49561 acatcaacta gtacagactt gaaaaaagct aatattatac tcttttctcc atctcctata
    49621 tttaaatcta attcttcttc ttttttcata tttccaccta catggtgagc actattacct
    49681 tcatttccag cagcaactac aataactgct ttctgtaatt gagaaaaagt ttgaatatac
    49741 ttttctaaca aactatttcc attatgagaa ccatcatttg tacttaaact tatattcaca
    49801 actaaaggtt tattaatctc attactttta tccattaaaa attttaaacc cttcatgagt
    49861 tgtgtactta atgcttctct taaactattt ccactagtta tttttaccat tgctatgcta
    49921 cttttataag caaccccata attatcaaaa tttatatttc ctccagcaca tgcaattcct
    49981 gcaacatgag ttccatgacc tgataaatca atttcaggta caatactaaa tggatcttca
    50041 gattttaaag cttcatttat tttattcttg tcatatacaa cttcattttc aagatcgtat
    50101 atatattcta tcctggtatt tccatcagta tctttaaaag cattatgagt atagtctatc
    50161 ccagtatcta aaaaaccgac taatattcct tctccagtta aattataatt attccaaact
    50221 gaaggaatac atgatgctct gttgctatca taagctgatg tatataatat tttaggtaac
    50281 tctatatact gaaggccatc aagctcaatt atcctattta agtcattaac ttttattata
    50341 agtattccaa atccatatcc taagtcttca acttttgctc ctatagcatc tactgaattc
    50401 ttgaatttga aaaaattatc cccatataaa acaactaatt ctatatcttc attattaggc
    50461 gaatattttt ctattaatct agttagtatt tgcttcggta ttatatttat aaaatctatt
    50521 ccacccttag ctttattctc catctctata ctcctcatta attattctat tgtactatta
    50581 aatattgata atatccttta ttttcttata gatattttat tcagaataca ttctttttga
    50641 taactaacat aaaaagtatt taaatataaa aaggacagtt ctaatttaga actgtccctt
    50701 ctatatttaa gtttataaat caaattctat tctataaagc ttagcaattg ccttaacttc
    50761 ttttatgtta gctgtctctg ttctcatgct accattatta cttattagta aagccttaac
    50821 gttatctagc tttttaattt gtctaataac tctttttcca ttaaattcaa ctaaacaaat
    50881 agaattattt tctatctctt ttattgaata gcataaagct aaatcattct cttgaattct
    50941 atatccagtc atatcattat tttctatttt taaataaaaa actttgttcg ctgtatgacc
    51001 atctatttta tttgaagcag tagcaagttg tttatatccc ttaacctgag ctaatgaata
    51061 atcatatatt ggaacatttt ttaaaactga tccaaaagct tgattccaaa cttcattaac
    51121 atctgctggt tttgtttgtc ttaattgttt ttcagcttta agttcttttt gaagttcctc
    51181 atcagtaaca accatattta tatcatttaa atcagtattt aaaacctttg atacctttga
    51241 tataagtgat tcatttataa tttttcttcc tgtttcaact tcatttacaa acttctcagc
    51301 tacacctagc tttttagcta aagctttttg tgtcattcca cttttttctc tagcctcttt
    51361 tattctttcc ccaactctac tcattttaag cttttcctcc ttgcatactt ctataccatt
    51421 ctctttcttc aattaaatgt tcaacaaggt agttaaattg ccattcttta gaggtttctg
    51481 taactacagc cattatagga ttttccgtaa taacttttgc tattgaaacc atcatttgta
    51541 attcttcatc agggaaatta ctgaatataa caactttttc atcaaagttt ttctttgaaa
    51601 caacttcaaa ttttaagcct tctaatatat ctttaacttt catagaagcc atttcattac
    51661 ttatattaat aatattgtgc cctgtcgctt gaaagtcttt tgtttccttt tcatttaaac
    51721 cataagttag aatagctttg ctattattaa gcatactcat gctaatctac ctcccataca
    51781 tcaattaaag tatttccggc tcttctatac cttctgcatt ttcaacagtt acttttgcca
    51841 taacttgtgg ttcatctggc atatcattgt aatcagtttt tacatttgct attttatcaa
    51901 caacatccat accttctata accttaccaa atgaagcata ttgaccatct aaatgtggtg
    51961 aattttcgtg cattatgaag aattggcttc ctgctgaatc aaaattcata gctctagcca
    52021 ttgatataac tcctcttgag tgctttaagt tatttggaaa ttggtttctt gtaaattcac
    52081 cttttattct atatcctggg ttacccattc ctgtaccttc tgggcatcct ccttgtatca
    52141 taaaccctgg tataattctg tggaatgtta atccatcata aaaacctttt ttaactaaac
    52201 taacgaagtt tctaactgtg tttggagcaa tttctggata taattctatt ttgatttttt
    52261 ctccagttgc catttctatt gtaactattg gattcatatt agtaattctc ctttcaaatt
    52321 taactattta gtattattta cttgaactaa aatgttaatc cttctttgta taaaattata
    52381 tattatttct cttaacaatg gaatattggg agaatattta aaaataaaaa taagacaaca
    52441 taaagaatcc taataacata gattctatta tgttgcccac ctagagttca acttaatata
    52501 atattttata gctttcatat aattcatatt ttcattttta agcaaaagta gttccagcta
    52561 caactcttga ataatcagta tagtttgata aattcttaac attaattttt gaaataatat
    52621 tatctcttcc ctctttattt atcatatcag taaataaaca agttaagaaa gtcgttgaaa
    52681 ctctacctat accaccaaaa tctaattcta cattttcatt taaattatct tcaatataat
    52741 ctcttaagat tatagcatct tcagcatgta tcttatcccc taaaaattct cttacattta
    52801 ttttcattgt aaaatcctcc ctctttaagt aactcctatt aatctttgtt caaattttct
    52861 gaaaattctc tttacattta ttataatatc aatctcttat taatttgtca acaatttaac
    52921 aaactttttt atagaaaaat ttcttaaaac aaaaaatcta cagaaatact aaaataatat
    52981 atactaaatt atcacaaata aagttatatg ttatttcata ttactgtaga ccattaatgg
    53041 tttatttaat ttctataagc tttttactta actctaatat tttattatct tctaaggaat
    53101 taaatatatc taaatcacct tttttcttta attctgattg catattattt tcttctaatc
    53161 ttctttttaa ctctcttata gtgcctgaac ttccatctat tatgtctacc ttttctccaa
    53221 caatatcttg tattacatct tttacaaaag gataatgagt gcatcctaaa actatacttg
    53281 aaatttctct atccatataa ggatttaatt tttccctcaa gaaattcttt acctcttctc
    53341 cctctaaatt tcctgcctct ataaattcaa ctaaccctgg acaaggaaga ggtattactt
    53401 ctctatgctc cccatattta tccataagat ttttgaattt ctcttgagtt aaagttgcct
    53461 ttgttgccat tactattact gagccagttt catgtaactt tatagcgggc tttaaagcag
    53521 gttctatacc tattataggt atatctttat aatactctct aagatcatga gctgctgctg
    53581 aagttgcagt attacatgcg attacaattg ccttagctcc cttttttatt aaaaattcaa
    53641 tagcattaaa tgaaagtgac cttatctctt gtggagtctt ctctccataa ggtgcatgct
    53701 tagaatcccc ataatatata taattttcat ttggcatcat ttctaaggct tttcttaaaa
    53761 cgcttaagcc tcctaagcca gaatcaaaaa aacctatagg tgcattcttt aaatcatctt
    53821 gcatcgctat ccctcacttc taagtacttt atttaataat cttaagataa ttttataact
    53881 ttctattaaa taaatctatt aaaattcata ttttaccttt atattaaaat ataaaatata
    53941 ttgacagtaa tattctattg taataaaatc tttttaagaa tatttacatg acgaatatta
    54001 ttaaataaaa atcatattat tgtacgttta aaataactat taatcataaa agtgaaagga
    54061 tgtgaacaga agattattgc aattatgtta atttattgta attgtattgc ttctaaataa
    54121 ttatgaataa agatttatta tttacaattc ctaaaaaata tcataataaa gaaagtttaa
    54181 tttctatttt tgaggaccac cctgaaataa aatttgtatc tttagttggg gttgacttag
    54241 caggtaatga tactgacgaa aaaattcctt ctaaactatt tttaaaagat atagattctt
    54301 tccttcatgg aactgccgta caaacagacg gttcttcagt tgtccttcca ggaatagcaa
    54361 ctttaaataa cgcaaaagtg gatatgattg ccgatttaga ttgtaagtgg tttgtagatt
    54421 acaattatga ttttatagac cctttaacta atagaccagt aggaacactt agaattcctt
    54481 gtttcttaat ccatgaagat aaagccgtgg actctagaca tatattaaaa aattcaattg
    54541 acacattcaa atcaaacata ttctcattat taaaaaaata tcctgaaaca ctaaatgatt
    54601 taaatataaa attcgatgat atagatgacc ttttattaac atctgcaaca gaattagaat
    54661 tttgggttaa aacaccaaac gataaggctt taattgaaga actttcaact tctcaagttt
    54721 tacaagaaca atattggaca agaactaagg gtaacgttag aactgcctta gaacaaagtt
    54781 tattatatat ggatgagtat ggatttgaac ctgaaatggg acacaaagag gttggtggag
    54841 ttaaagctaa gcttacttct tcaggtaaat tcgatcatat aatggagcaa ttagaaatag
    54901 actggaagtt ctcagatgct atgcaagctg cagataatga attatttata agaactttag
    54961 ttaaagaaac ctttagaaga aacggattag aagtaacatt ccttgctaag ccaatagatg
    55021 gagttgctgg taacggagag cacactcact taagcttaag cttaaaacta aaagatggaa
    55081 gaattataaa cctatttaat ccaactaaaa atcatttctt aagcaaaata ggatatggtt
    55141 ccataatggg tatattaaag aactacgagg taatgaatcc atttatctca gctacaacag
    55201 actcactaaa aagattaaaa ccaggatttg aagctcctgt ttgtatagtt acatctctag
    55261 gtcaaagccc tgaaattcca tctagaaata gaactatatt agcaggatta ataagagatc
    55321 ctcataatcc tttagcaact agatttgagc ttagatcacc aaatccattt acaaatactt
    55381 acctttgtat agcatcaagc tatatggcta tgttagatgg tattaagtat gctttagaaa
    55441 ataataaaac agaggacgac cttttagctg aattatctaa aaaaccaggt gaagaagcag
    55501 attacctaga aacatctaga gcatatagaa gtgaagaaga tgtatttgaa gatttcacag
    55561 actcacaaag aaatgaatac tttggtgtag ctcctgctac tgtttttgaa aacctaagtg
    55621 cttttgataa ataccctgaa aaggtagagg tattaaaagt aaattcagta tttacagaca
    55681 agcttataaa tagtttcaaa atggctacta ctaagagatg gactacagaa ataacttcaa
    55741 gaataattcc ttcttacaca aaagatataa gagccgctaa acaacttcat tgttgtgata
    55801 aagccttaga tcttgacgta tcaacttgga tgacaataaa tgaattaaga catataataa
    55861 tgaaagattc ttatcataga agaagcttat ttactcaaat aaagaacgct ataaatgaat
    55921 ctgattttga aaaagcttct gatttacaaa taaaacttga taaaaacatg aatgaattaa
    55981 atgatcttta ctcaacttac aagaaaaatt tattagatat ataataaaaa agaaatcaat
    56041 gctttaaatt gatttctcat aatttgaaga aatgaccttt gttaatttca aaggtcattt
    56101 tatattatta tattaatgat atgtatttcc taaactaatt gaatttttac ttaatatttc
    56161 aaatgaatag tcttcataaa aaacaaatgg attattattt ataatttctt ctgcttcttt
    56221 aaaattatta gcatggaata ttatagttcc tctatttctg aaatatgacc caccacataa
    56281 tacatgcttg ttaacagctg tatctttata cattttggct ttatgctcac cttggaatct
    56341 attatctcca ttaattttat aatttatttt tacaaacata ccgctgctct tagtcatata
    56401 aattaacctc ctaaaatttg ttatatgcta ttatattatg ttaatgtcct atatattcct
    56461 aagctgtcta aaaaaataag aacttatgtt ccctaatctt ttttaatttt ataaaacata
    56521 tgttcgcttg tcaattaaaa aaaggatgta atcattcata ttacatcctt ttttctataa
    56581 aattctttat ttatatgtat atttctctat ttttttaatt atagtaattc ttatctattc
    56641 aacctcttct atctcatgat ctcttttata aagtctatat ttaataaagt aagctatttt
    56701 acaaataaaa taagcaatga atataccaac taaatttata aatatatccc ctaaatcaaa
    56761 gaatcctctt ttaaatacat attgagcaag ttctatatta aactctatag aggcagataa
    56821 tataaaggct aatacaaagt tatattttct tagcacatat cctataggca taaagaatat
    56881 tatattacca attaaatata actgccctgt tctatcccct cgtagaccat gtaaaactgc
    56941 aaatggatct aactgaaatc ctctggccat tttaggtcta gcaaataata gtataaacat
    57001 tgttcctgca tataaaccaa ataagaataa tttaaatagt tttgaagttt tcatcctagt
    57061 tgttgttaag aaaacttgat atataagcat tacacttgtc aacttaaaaa agttatatat
    57121 tacaaaacct tttttaccac tcaaatctat ttttataaaa acatctatgc attcctttac
    57181 tattaattta taaaatgcca ttgctaaaaa tactgaaatt ataagcaaaa tactttttat
    57241 aaaaaaattc tttaataccc tcttagctgt ttccacaaaa atcaccccca ataatttata
    57301 aattttatat tgaatattaa tatttttata gtataataac taagtgcttt ttaaaacaag
    57361 cctcttatat tattatactt taatttaata acatgtctaa aatcaaaaat ttatccacag
    57421 aattattttt taaaaattat ccacagcaac tcaaaatatt ttttatccac aaaataaata
    57481 atctatattt cttcacagta tttttctttc aaaacaaatt ttaatacact ttacccacaa
    57541 aaatactaca attatattac catttaaatt tatattgtat ttataataaa attttcttat
    57601 tttaaaaaga taattatgaa atttttatcg tcaaagggct cagttagaat aaatcaataa
    57661 cttttattaa aatttatcca caaagaaatt attttttctt aaaatacttt aaaaactaac
    57721 tgagccctaa ctaaaaggta aattttaaac aacacataac taaaaagtag atttattata
    57781 tgaaaaatat aattttcact ttaaatcttt aggtataata aaaaaataaa agtcctattt
    57841 agcttcaggt aaccaaaact aaatagaact tattttatat tattaatact ttatattaag
    57901 ttaaatactt ctaactctct attatgtgct ccttcttttt gtaactctac tagagcttta
    57961 agagtatcta atgaagtcat aactcctata tttctttcta ttgccgctct tcttatatgg
    58021 aatccatctc tcttagaatc attagcttta gttggagtat ttacaactaa atctatttct
    58081 ttattcttta ttaaatccat aatgtttgga gattcttctc ctattcttct aacttcctta
    58141 gcatctattc cagcctctct taattcatta gctgttcctg ttgttgctat gaaattatat
    58201 cctagtttat ctaaatcctt agcaagttcc ataaactctt ttttatcatg tttcttaata
    58261 gtagctaaga ttgttttacc cttatctcca gtgtacattg aagccccaac aaaacctttg
    58321 tataacgctt caaaaacatt tcttcctact cctaaaactt ctcctgtgga tctcatttct
    58381 ggtcctaaac ttacttcaac attaggaagc ttttgagttg agaataccgg aaccttaact
    58441 gatactaact caggctcctt ataaactcct gtaccatatc ctaaatcttt taatttttct
    58501 ccaagcatta ttctcgtagc tatatctact attggaactc cacttacctt tgatatataa
    58561 ggcactgttc ttgaagctct tggattaact tctattacat aaagctttcc ttcaaactca
    58621 ataaactgaa tgtttatcat tccttttatt cctatgctta aagctaattt cttagtgtaa
    58681 tctaaaacat cttcctttat cttatctgaa atattttgag ctgggtacat agtaatacta
    58741 tctcctgagt gaactcctgc tctttctaaa tgctccataa ttcctggaac taaaacatct
    58801 tcaccgtcag atatggcatc aacttctatt tctctaccca ttaagtattt gtctataagt
    58861 attggattct tggaatcttt ctcaaaagca tttgttaaat agtatgttaa ctcttcttca
    58921 tcatgagtta tttccatacc ttgaccacct aaaacgaagg aaggtctaac taagattggg
    58981 aatcctaatc ttcttgcttc ctctaatcct tcttctaagc tccaaactcc ttttcccttc
    59041 ggtctagcta tattaagtct ttctaataaa tcatcaaatt gttctctatc ctcagctagg
    59101 tctatttgat ctgaagtagt tcctaatgta ggaatattct tctcctttaa gaacttagca
    59161 agcttaatag ctgtctgacc accaaattga agtataactc catctggctt ttctttgtct
    59221 attatgttta aaacgtcctc ttcagttaat ggctcaaaat aaagcttatc tgaaatactg
    59281 aagtctgtac ttactgtctc tgggttattg tttataacta ttgtttcaat tccttgcttt
    59341 ctaagtgcca ttacacagtg aactgaagca tagtcaaatt ctatcccttg tcctattctt
    59401 ataggacctg atcctataac tacaactttc ttattatcag aaacaactac ttcgtcatat
    59461 tgttcatatg ttgaataata gtatggtgat aaagcctcaa actctccacc acaagtatca
    59521 accattttat aagaaggttt tatatgccat atatctctta atctgtagat ttcatctggt
    59581 gaaaccttta gcatatcagc tattgcctta tctgaaaaac ctcttctctt taattttaat
    59641 aaccactctc tatttaaatc atctattgta ctttgcttaa gcttttgttc ttcttcaact
    59701 agccatctga atttttctaa gaagaatata tctattccag ttattttaga aaccatatct
    59761 attctgtatc ctcttcttag catttcagct aaagcaaata ttctctcatc atctggagaa
    59821 actacccttt ctcttaactc atacattgaa agatctttaa attttttatg ttctaatgaa
    59881 tacttaccta tttctaatga tcttataccc tttaaaaatg ctgcctcaaa attacttcct
    59941 atggccatta tttctccagt agccatcatc ttagttccta aaaccctatc agctccttgg
    60001 aatttatcaa aaggccattt aggaattttt acaactacat agtctaatga aggctcaaaa
    60061 caagcatatg tcattcctgt tactgcattt tttatttcat ctaatccata tcctaaagct
    60121 attttagctg ccaccttggc tattggataa ccagttgcct ttgaagctaa agctgaagat
    60181 cttgaaaccc ttggattgat ttctattact gcatattcaa atgaatgtgg atttaaagca
    60241 aactgaacat tacatccccc ttcaattcct acagcattta ttatatctat tgatgcactt
    60301 ctaagcattt gatactcttt atctgataat gtttggctag gagctacaac tattgaatcc
    60361 ccagtatgta ttcctacagg gtcaatgttt tccatgttac aaactgttat acaattacca
    60421 aaagagtctc tcattacttc gtactcaatt tctttccatc ctttaacact cttctctaaa
    60481 agaacctgtc caattgtact taattgtaat cctgatgtta atgtttctat taattcttct
    60541 tcattattag ctattcctcc accagttcct ccaagagtat atgcgggtct aactattact
    60601 gggtatccaa tctttctagc aaaggcaata ccagaatcta aatcagttat tatttcactt
    60661 tgaataacag gttgattgat tctattcatc atatctctga ataattctct atcttcccct
    60721 tccttaatag attctatgga agtacctatt acttttacat tatatttatc taaaattccc
    60781 ttttcataaa gctcaacagc aaggtttaac cctgtttgtc cacccattcc tgctaaaaga
    60841 gaatctggtc tttctttctc tattactttt tctacaaatt caactgttaa aggttcaaga
    60901 taaaccttat ctgctatttc cttatcagtc attattgtag ctgggtttga gtttacaagt
    60961 acaacttcaa tcccttcttc tttaagagct tggcaagctt gagtacctga gtaatcaaac
    61021 tccgctgctt gtcctattat tattggacct gaacctatta ctaaaacttt ttttatatct
    61081 ttatttaatg gcatattact tcctccttat agtgcatact tcataaactc atcaaaaatg
    61141 tattcgctat cctttggtcc tggacatgct tctgggtgaa attgaactga gaatattggt
    61201 aattctttat gtttcattcc ctcaactgtt ccatcattca tacttacatg agttatttcc
    61261 atattttctg gtaaagtagc aacatagtat ccatgatttt gtgatgttat atgaactctg
    61321 ttatttatta aatctttaac tggatggtta catcctctat gaccaaactt aagcttctta
    61381 gtttctccac ctaaggttaa agctaataat tgatgtccta aacaaattcc tacgattggt
    61441 tttttcccaa ctattttctt tatctcattt actgcttctc ccatatcctc tgggtctcca
    61501 ggtccatttg acaaaaatac taagtctgga tttatgtcta aaacttcttc tgctttaaag
    61561 tcataaggga atactgtaac attacatcct ctttttacaa agtttctaat aatgttttgc
    61621 tttattccaa agtcgattat agctacattc tttccttctc ctgagatctc atatttttca
    61681 ttagttgaaa ctattgaaac tgcatctcta tttgagaaag cttctaactt atctttaaca
    61741 tcttctaagt ttgaattatc taaaactata attcctttca ttgttccatt atttcttaag
    61801 atttttgtta aggctcttgt gtctatacca tctaatccta aaaccttatt ttgagctaag
    61861 taagtttcaa gctctaattc acatctaaaa ttattgggat actgacattt ttctctcact
    61921 atgaatcccc ttactttagg ttccttagat tctaaatcct ctaagttaat tccatagtta
    61981 cctattaagg gataagtcat agttactatt tgtccataat aagaagggtc agttaaaacc
    62041 tcctgatatc ctgtcattcc tgtgttgaaa actacctctc caacacactc ctttaaatat
    62101 ccaaaggctt taccttcaaa tactacgcca ttttctaata taagctttgc tttcatttct
    62161 agacctccta tttaaaatca tatttaaaaa acacacttta aaaggtctta attccattgc
    62221 catcattcta aatgttattt aattaaaaga aagaggtact agagcgattg cgtaaaaaat
    62281 caatcactct aatacctctt tttctctttt atatggaatt gtattaaaat acaaagctat
    62341 ttcatttttt aatttcatct gtttttttgt acaacaaaaa attctattta acataactat
    62401 acctctttct ggcctcacgg gaccaattta aagtgtattt ttatcttatt gataaattct
    62461 tattatcaat tttagtttat taaatttata tagtcaagtc cattttttaa atttaacaag
    62521 ttttaacaac aaaatttatt ttttccataa aaagttaagg tgctagatgt aaaacaccta
    62581 gcaccttaac ttaatttcaa acctatacct acttcattgt cctattgaag caattataat
    62641 tattatacat atatgtcatt ttgtcaatgg aattcattga aattatataa ttatttcgtc
    62701 aattctctac aaattttata attattaagt tctattaaaa tctttataaa attcaccttt
    62761 gtataataaa aagcatagtt taatactaaa taaaaatttt ataggagata cttatggaaa
    62821 taatttatcc aaatgatttt gttagatttt ttaatgagga tgaagaaata gatgaaatag
    62881 gcgagtttga agtcttacct gaggatacag aaaactcctt tgaaagatac tacactggta
    62941 atgatgatac atttaattat aactatccta tgaattatag aaattctgac aatgattcta
    63001 aattctcagt aataccacca gttattaaaa atattcctga taatattgca aatgtacctg
    63061 ctgttataca agacttacct tatgcaataa aggatatggg aaatgactta atgaatagtg
    63121 cccctgttgg atatatatca aatatgcttc ctataccaac taattggcaa agtcctcaat
    63181 caccttctcc aatgggacca cctccatctt atataccatc aaaaaaagat acaggtgttc
    63241 aaaactttgg taaagaaagt ggaaatctaa atgcaaaaaa tgttagttct agctctataa
    63301 gattttgtct atatagattc acttatattt ggcaaactaa cggaagaagc tattgggctt
    63361 acttaactag agttgataaa aaatcagttt ctggatggcg ttgggctggc tttagatggg
    63421 tttattttgg agttgattta aaaagaattg attcctttat ttgttactaa ataataaaat
    63481 attatttatt tcaaactttt atcccttaat ctactaaaat ctatttaatc tattattttt
    63541 ctaaactaaa agcctttact aattaacttt aatcatctaa atgttcttat tccccctata
    63601 ataacatttt taaagttaat tagttaaagg cttaatttta tttttagtat gatactaatt
    63661 ttatataact aaactttcct actctatttt taatctttta tattttaaat tagaattttt
    63721 tatattactc atttttagat aaatttaaat cctcaaattt aatatcaact aaagtatctt
    63781 ttatcttctc agaagaggat aattcgccat atttatcaac ttttttgcta ttcttaatat
    63841 catgatttaa ggagcctggc ccatttatac atccaccttt gcaagccatt ccttctaaaa
    63901 agtttccctg tgctcttttt agttttgcta atctaagttt tatgtcgcaa tccttaattc
    63961 catctccaag tataggtcta aaatctactt ttatcccttc gctatctata agatgtttaa
    64021 ctgattcggt aactcctcca cttctagcaa aaagtcttcc ataaaatgaa ccatgcttag
    64081 tatcactttc tttacattcg tcaattttaa tatttctaga gtcaagtaaa gctaaaagct
    64141 cctcaaaggt caatacataa tcaacatctc cactaacctc atttctctta gcttctgttt
    64201 tctttgcaat acaaggtcct atgaatacag ttttaacatc tttattttga gattttatta
    64261 accttgccat agctaccata ggagatacag tattagatat atactcctta agctctggat
    64321 aatttttttc tatataagaa acaaaagcag ggcaacaact tgttgtcatg aagtctaatt
    64381 cacctttttc tttaaattca ttacattcat gaacagccac caaatctgct cctaaagctg
    64441 cttcaaacac atcttcaaat cctaattttt ttatagcagt aatcacttta ccaatcttac
    64501 tatgattaaa ttgagatgat atagctggcg ccacaatggc ataaactttc ttctcatctt
    64561 taagtaatct tataacatct actaaatagg atttgtccat tatagctcca aaaggacaat
    64621 caactacaca ggctccacat tgaatacatt ttgaatcatc tatgacagct tttttactat
    64681 caacatcata agataaggcc tttggaatac atgctctcat gcaaggtctt tttacctcag
    64741 ctatagcatt gtaaggacaa acctctttac attttccaca ttccttacat ttttcataat
    64801 caattttgca ttttctatta tcaaaattta tagcttcaaa attacaacta tctctacatt
    64861 tcttagctag gcatcctcta catgcatcag tgactataaa tctattaaca ggacattcat
    64921 cacaggctat gtctattact tctatcatgt tttctctatt ttcatgtcca cctaaagcta
    64981 gtttaattct ttcttcaact atagccctct ccttatatat gcaacatctt acattaggtt
    65041 ttaaatcatc cacaatttct tttgctatat taagcttttc tttttctaaa gtaccatcaa
    65101 attctttctc tgctatcctt tttaaaacct catatttaag aacttgaaca gcagtgtcaa
    65161 actttatata ttgcttgtta gcatccttta tagccataaa actcccccac aaaataacta
    65221 ttgtagcgta gatattatat tgagatttaa agaaaatact atttattatt ttttagagac
    65281 aaatttatta tccttaatat aaaatctcca taaaaaatcc ctagcttcct cagcataatc
    65341 tatacctatt ctcttggctt caacaataga aaaatcctct ttaattgagg aatcatcttc
    65401 aacataaatc tcttcgctac taatagtatt tatgccattc tcccccttat ctattcctaa
    65461 agccatgcat aattttgatg gaccattaga aaaatttttc ttttcgtagc ttgataactc
    65521 ttcataattt ttcctatatc ttaatttact catttcttct attccctcta aaggttcaat
    65581 tcctcttata agtactcctc ctgccacgtc tttttcttca cttattagat ttaggcagtg
    65641 atacattcca taaattatat aaacatatac agttcccgga tctgcataaa gagtttctgt
    65701 tctattagtt ctcttgccac cgtaagcatg agaagccttg tctatagctc ctatataagc
    65761 ttccgtctca acaatctttc cttttaaagt aactccatta atatttctaa ctaaaacttt
    65821 tcctaaaagt tctttagcta cagttaatgt atctctatta taaaaatctc ttcctaatct
    65881 cataaagcac ctcttccata attctctttc atgaaaatat tagttataca attttatctt
    65941 tacttactaa ttataattct atacaataac ttctcagttt tataaatcct ttaaatttaa
    66001 tagtaaaagt atatcttttt acttctaata aattgaatat taaatctaaa aaaacatcta
    66061 attatcataa aattattaat aacaaaaaat aaaaagcacc ttaacaatag tttaagtgct
    66121 ttttcttata taatatcttt ttatttgatt ttacataata aaatataaaa tttatatcat
    66181 tactaaaaat ttttttgatt taatttttca tacaaatcta ttatttcatt tgctaaatct
    66241 tttaatgtca tagtagtcat caattgatcc tgtgcatgaa ttaatagcat ggatatttct
    66301 attttgttgt cactagcctc atcttgaatc aaagctgttt gtgatttatg agccttacct
    66361 aattgtattg atgctttttc tattaagcgt ctagcccctt ctacatcttc tgctttagca
    66421 agctgaatag cttccatagc ataacttcta gcttctccac tatgaattat taaatccatt
    66481 attatttctt tcattttccc atacttcctt ttttcataaa ttaaaatctc ttcaccctta
    66541 aatacctaaa atatttcttt aatatataaa attaacctct ataagcttat ataatctatc
    66601 taacttgacc atttccataa attatatact tatttgttgt taattcctta agtcccattg
    66661 gtcctctagc atgtaacttt tgtgtactta ttccaatttc agcaccaaat ccaaattctc
    66721 caccatcagt aaatcttgtt gaagcattta catatacagc agctgcatca actctttgaa
    66781 ggaatttctc tgaattaaaa taatttttag tcactattga ttctgaatgt tttgttccat
    66841 atttatttat atgagagata gcctcatcta aatctttaac aatcttaact ccaaggatat
    66901 agtctaaata ttcttttccc caatcctctt cagttgctaa gtttatatca ttcacaatta
    66961 ccttagtttt ttcacaacct cttatttcaa cgcctttttc ttttaaagcc tttattgcta
    67021 ctggtaaaaa ttcttccgct attttctcat taactaatag tttttcagca gcattacata
    67081 cagcaggtct tgatgtttta gcatttacta ttatatctac gcccatattt atatctgctt
    67141 catcatctat gtatatatga caatttccaa ctccagtctc tattacaggt actgtagcat
    67201 tatttactac ggcttttata agtcctgctc cacctcttgg aattaaaaca tctaaataat
    67261 catttaatct catgaaatct gtagcagttt ctcttgaagt atcctctaat atttgaactg
    67321 acccctctgg aagacccgct tcctttaagg catcactaat aatatcaact atttttaagt
    67381 tagaatttat agcctcacta cctcctctta aaataacagc gtttcctgtt tttaaacata
    67441 gggcagcagc atcagcagtt acattaggtc ttgcttcata tattattcca actactccta
    67501 atggaactct ttgtacacct attctaagac cattaggtct cttaaacata gatgtcactt
    67561 ctcctattgg atcatctaat ttaataacat ctgctacagc atttgctata gattttactc
    67621 tttcctcatc taattttaat ctatcaagca ttgcattaga tgttcctttt tcgattgcct
    67681 tttcaatatc ctttttattt tcttctaata tctcatcaca tctttttatt aaggattcac
    67741 ttatttttaa taatccatta tttttttcat tagtagatgc aaatgaaagt gtgtaagaag
    67801 cttcttttgc ttttttacct tttataataa gttcattatt catttttatt cccccttctt
    67861 agctacaaac atagttccta tatcttcacc atttaatatg ttaattaata tttctggatt
    67921 ttctccatta gctaaaatca tatctattcc attacttgtt gctaccttag ctgctgaaag
    67981 tttagttacc attccgcctg ttcctctatt acttccagct ccccctgcac attcttctat
    68041 ttcaggagtt atttcataaa cttctttaag catcttacta tcctcattag ttcttggatc
    68101 tgaatcgtag aaaccatcta tatcagataa tattattaaa gtatctgcat ttactaaagt
    68161 agaaactata gctgaaagat tatcattatc tccaaagttt aatatgtttt gtatctcatc
    68221 tattgatata gcatcattct cattaactat aggtataatt cctttttcta acaaagtatc
    68281 aaatgtatta catacgttat ttttcattct ttcattttct agtacatctc tagttaaaag
    68341 aacttgtgcc acaacatggc tatattctcc gaaaaattta ttatatatat gcattaaagc
    68401 acactgtcca acagcagcta cagcttgctt ttctcttata gtctctggtt ttttctcaag
    68461 tctaagtttt ccaactccaa ctccaattgc tcctgatgaa acaagaacaa cttcttttcc
    68521 catattacat atatctgaaa ttactctaga taatttttct attctcatta agtttacact
    68581 tccattttca taagttaatg tagaagttcc aaccttaaca actattcttt tactatcttt
    68641 taatgctttt ctcatttgta ggtttccccc tttttattta taaaaattat ttttccatta
    68701 aatcataagt atattatatt ctaatacgtt aaatcatgaa actacccatt tataatttct
    68761 ccaccattta cgtgtattgt tatacctgat atgtatgatg cttcattaca agctaagaaa
    68821 acataagctc cagcaagctc tactggttgc cctgctcttc ccataggtga attaactcca
    68881 aattcagaaa ctttcttttt attcattgaa gatggtataa gaggtgtcca tataggtcct
    68941 ggtgccacag cattaaccct aattccattc ttaactagca tcgttgctaa ggttcttgtc
    69001 attgtggtta aagcaccctt agtacatgaa taatcaaaaa gtatctcatt tcctctataa
    69061 gctgtaatag aagtggtatt tattattgaa cttccacatt ccatgtattt aactgctgcc
    69121 tttgtcatat agaaagttcc aaaaacattt attttaaaag tattttctat ttgctctggt
    69181 ccaatatcat ctaaagtttt agaatcatgt tgaactgccg cattgttaac taatatatca
    69241 agttttcccc atttttcaat gaccttcttt acacttgaat tacaaaaatc aatatcagaa
    69301 atatctcctt ttataactaa agcctcagaa ccagcacttt ctataagttc cttagtctta
    69361 ttagcatcct tatcctcttc atctgataga tatattatac aaactttagc cccttctctt
    69421 gcataaccta aggctacagc tcttcctatt ccactgtctc cacctgttat tatggctacc
    69481 ttattcttaa gtctttttcc atcatttata tactgtggta aatcatatat tggtagtgga
    69541 ttcatttcat cctctactcc aggatgaata ctttgatttt gaactggaaa tgttgttggg
    69601 aataattttt cattgctcat aaataatttc ctccttaaaa actttatttt aaatttaatt
    69661 atttataagc ttattattct caattatttt ttacttatag ctttattttg tattttcttt
    69721 actatctttg catttaaagt tgtgggagac actttagata aaaatactaa taccttattc
    69781 ataatcccag gaatacatat tgccttattg tttatcaaag ccttatagcc acactgagcc
    69841 actttttctg cactcattgc cacatctaaa tctccctttc cagctctctt agaaaaattc
    69901 gtcttagtag ctcctgggca aagagtaacc acattaattc ccttatcttt aacctcttct
    69961 cttaaagcct cagaaaacga taaaacatat gctttacttg cataataaac taaaattaat
    70021 ggacctggtt gataggcccc agtagaggca acatttaaga ttttcccttc cttttcattt
    70081 atcatatctt ttaaaataag cttagttaat ttggttaaac ttataatatt tacatttatt
    70141 acatctaatt gcttttgcca atctatttca ttaaattctc cattaaatcc tataccagca
    70201 ttatttatta atatatctac ttttcctact tcagttttaa tttttttata taaaaaatct
    70261 atgttttcac ttaaactcaa atcaaattta tatatgtaag ccattacaca ttttttcttc
    70321 gaaaaatctt ctaattccat taatttttct aaatttcttc ctactaatat tacatcataa
    70381 ttgttttcca taaatatttt tgaaaattca tatccaattc cactagtagc ccctgttatt
    70441 aaaactgttt tcttcataaa aattcccttc ttataaataa atccttaata aaacatttat
    70501 tatgtttttt gctaatatgt caagtttttt tctgtaaacc tttatttaaa aaacctttat
    70561 taaacgttta acatatgata taataacatt tgcatcgata gatgtgggag ggtgagggag
    70621 atatttttga aaaggagaaa ggatgaataa cttttttaaa cttaaagaac atgaaacaaa
    70681 tataagaaca gaattacttg ctggtcttac ctcatttttt gctgcagttt atataattgt
    70741 agtaaatgct agcatactta gtgatggtgg agtatctatg gaaccactta taatagctac
    70801 agtattagca tcattaattg gatgtgtact cgttgcactt ttaagtaatg ctcctcttat
    70861 tataatgcct ggtatgggta ttaatgcact atttacttat accatagtaa atactatggg
    70921 acttacattc caacaagctt tagcagctgt tatagtagct ggtattttgt ttattgtggt
    70981 agcggttaca cctttagcag atatgttaac tagctcaata ccaacttcat taaaagaagc
    71041 aatcacagtt ggaattggta tttttattac tttcttagga ttacaaaagg ctggcttaat
    71101 agtaggagat gcctcaacac ttgttaaatt aggcgatatt tcatctaaag aagttatagt
    71161 attcttcatc actatgatac taatgctagt tttatttata aaaaatgtac caggttcatt
    71221 cttaatagga attttatctg gaacagttat atcaatgtta tttggaattg ttaatttatc
    71281 aagttttcaa ttatcattac ctaatttcag tgaatataaa caagttttct tatcagctaa
    71341 ttttagtgga atagcttcaa tttcattttg gattgcaacc ttctcactaa ctttagtcct
    71401 agtatttgaa aacattggtt tacttcatgg acaaattgat ggtatgttaa aacaaccaag
    71461 taaaagacaa agagctctta acgctgttgc ctttactact ataatttgtg gtatatgtgg
    71521 tactagtcct tcagtttcaa ctgtagaagg tagtgcagga atagcagctg gtggtaaaac
    71581 aggattaact tctatattta caggattttt atttttaata tcaatcttct ttataccagt
    71641 tatatcactt attccaaact cagcaatagc tccagtctta ataataattg gaggcttaat
    71701 gattcaaaat atagtttcaa taaactttga agacttcact gaatcattcc cagcattcct
    71761 tactttaact ataatacctt taacatttag tatagttgat ggtattgctt ttggatttat
    71821 agcttatcct atttgtaagt tagcaacaaa gaagtataaa gatgtatcta tccctatgta
    71881 cgttatatca gcaatctttt tagtatattt tgtacttcac gctacacaaa tatagcttta
    71941 aattataaat taaactccct tattaatatc aataagggag tttttttata tactgattat
    72001 tattctccta agaacatttt catatcatct tctacatttg taattccacc aattccaaag
    72061 ttctcaacta aaactttagc tacgtttggt gataagaatg ctggtaaagt tggtcctaaa
    72121 tgaatgtttt taacacctaa gtgtaataat gataataata ctattacagc tttttgttca
    72181 taccatgcta tgttgtagct tattggtaat tcatttacac tatctgttcc aaatacttca
    72241 gcaagtttta aagctattac aactaatgag tatgaatcat tacattgtcc tgcatctaaa
    72301 actcttggaa ttccattgat atctccaagt ggtaatttat tatatttata tttagcacat
    72361 ccagctgtta atataacagt gtcttttggt aatttttcag caaattctct atagtaatct
    72421 cttgatttag ctcttccatc acatccagcc attacgaaga atcttcttat tgcacctgat
    72481 ttaacagcat caactacttg gtcagctaaa gcaagtactt ggttatgagc aaatccacct
    72541 acgatttccc ctgtttcaat ctctgttgga gctttacaag tcttagctaa ctctattatt
    72601 tgtgagaaat ctttgtgtcc attttcatct gctgttatat gaatacatcc ttcatatcct
    72661 acagcacctg ttgtaaatat tcttcctcta tatgaatctt gaactggtgt tatacagttt
    72721 gttgtcatta atattggtcc attaaatgat gcgaactctt tattttgtaa ccaccatgca
    72781 ttaccatagt ttcctgcaaa gtgagcatat ttcttgaatt ttggatagta gtgtcctggt
    72841 aacatttcac catgagtata aacatctact ccagttcctt ctgtttgttc taataacatt
    72901 tctaagtctt ttaaatcatg tcctgatatt aatattcctg gattatttct aactcctata
    72961 ttaacttttg ttatttctgg atgaccataa gttgaagtgt tagcttcatc taataacgcc
    73021 attccatcaa caccgaattt tcctgtttct aatgctaaag cagttaattc atctgcactt
    73081 aatgaatcat ctaatatttt aactaaagtt tctgccataa atgcatgaac ttcttcttta
    73141 tcatgtccta aattcatagc atgctccatg tatgctgcta atccttttaa tccatacata
    73201 ataagctctc ttaaacttct tatatcttca ttctctgtag ctaaaactcc tacttgtgaa
    73261 gctttttcca tcatttcctc actagttgta gctgtccaag ttgcacatcc tttgaagtct
    73321 gatccaattg gatttccacc attagcttgt actctttctt ttaattcttc tcttgctact
    73381 aaagtatctt taactctttt ttctattgct ttaaagtcaa agtttgcatt agtaatagta
    73441 ataaataagt tatctattat cattctgttt acttttttat ctactatgcc tacttttctt
    73501 ccttcattag ctactgtagc taatccttta gtaacaaaaa ttaaaaggtc ttgtattttt
    73561 gctacatctt cagtttttcc acaaacacct cttacagtac agcctttgca tcctgctgtt
    73621 tcttgacatt gaaaacaaaa catactcatt ttaaattcct ccattttttt atttttatat
    73681 gtaaaattct tttaagattc ttttttatta gtaactttct tagtcacctt acatctaaaa
    73741 ttatacacag aatttttaag taactcggta actaaagtta cctatttaat aaatttattt
    73801 cactttataa aattatgtat ataaatctta atttattgaa tatttataca ttattcatta
    73861 tttttatgca tttttcattg attttataca tttcattgtg tataataggc tttgtactaa
    73921 ttgatttgtt attaaaaagg gggactaaaa ttgaaaacta aatataaatc gtctgattta
    73981 ttaaagtttg taattccatc tttattagga gtaattttat ttatggttcc tataaatgat
    74041 ggtggaaata taacaatacc agtagccttt ttcacaacta aacttaagga tttaataggt
    74101 gattatttac ctactttatc aatgatagtt gttgtaatag ctgcttcatt aacaataata
    74161 actaaagcat ttaagccaaa atttataata gaaaataact ttttaaattc attattaaat
    74221 gttaattgga tttggacact tggaagaatc ttaggtggat tatttataat atcagcattt
    74281 actggatttg gacctgaaat aataaagtct gatttaactg gggcatttat attaaatgat
    74341 ctactaccaa cacttatagt agtattcttc tttgcaggat tattcctgcc gcttctttta
    74401 aactttggtt tattggaatt ctgtggtgcc ttactaacta agataatgag accattattt
    74461 aaactacctg gacgttcatc aatagactgt atgacttctt ggttaggtga tggaactatt
    74521 ggggtattac ttacttcaaa acaatatgaa gaaggatttt atactgagag agaagcttgt
    74581 atagttagta caatgttttc agttgtttca ataactttta gctttgttgt tttatcacag
    74641 gttggattag agaatatgtt tattccattc tatttaactg ttacttttgc cggaatagtt
    74701 gccgctataa ctcttccaag agtttggcct cttagtaaaa aacctgatgc ttatttcaac
    74761 aatgcagaac ctaagaatgt agaagatgtt cctgaaggat ttactccttt cacttttgga
    74821 gtttttaaag cagttgaaaa agcccaaaat gaaagtagtt taaaaaaatt ctttgctgat
    74881 ggaattaaaa acgtacttga aatgtggata ggagttttac ctgtagttat ggcaatgggt
    74941 acattagctc ttatgatagc tgaatatact cctatatttc aatggcttgg tgttcctttc
    75001 attcctttat ttaaaatttt aagaattcct gaagcttcag ctgcttctca aactgttata
    75061 gttggatttg cagatatgtt cttaccatca gttatagcat caaaaactat tttaagtgat
    75121 atgactaaat ttgtagttgc ttgtgtatca gtaactcaac ttatatactt atcagaagtt
    75181 ggaagtgtta ttttaggatc aaaaatacca ctaaacttaa aagaattatt tatgattttc
    75241 ttaatgagaa cactagtcac tctaccagtt atagcactta tagcacactt attattctaa
    75301 aaagtaaaag ggcgtgtatc aaaataactt ttttaaaact tacaaacata ttccgagaat
    75361 tactagattt cgctaagaa
//
LOCUS       DAEVGI010000003        63157 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEVGI010000003 DAEVGI010000000
VERSION     DAEVGI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 63157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63157
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.003"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            180..1967
                     /locus_tag="K8M77_000157"
     CDS             180..1967
                     /locus_tag="K8M77_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI7084688.1"
                     /translation="MENRKDIYEKRIEEYSSALKRLKRDYNIISALRLVVSLSILFFI
                     YYAYSIGSISFGIILFLLNSILFLYLAKVHEGIVNKISKREALIEVNKKEILRLEGKW
                     REFNDLGEEYLDNKHPFINDLDIFGKNSLFQWINETGTVYGREKLSHLLKLEELPNKE
                     EILLRQEALKELSKKVDFRHEFIASLKDKKGKKEKYLGEWLKEDSKAISPLLNILRII
                     MPVINIGITILVGMNVISWQILLISLVISYGILKLGNKEVIKGLNIFEDLKYRIKTYV
                     EALELIEKENFQSNIIKSIKSNLDMNGKSASKELKSLEKITSWLYDRGNAFYLLLNCY
                     LLWDYQILSKLEKWKSSNKDEFYKWMISLGDFEALVSLAGFTYNNHGWATPKINDDYT
                     LKGKNLSHPMLGEKGVGNSFDINKDKRVILITGSNMSGKSTFLRTVGFNCILAYLGLP
                     VKGESFEAPILKVYTCMRTGDNLEESISSFYAEILRIKIIVEGVKRGEKILFLLDEIF
                     KGTNSLDRHEGAEILINQLLEGNTLGLVSTHDFELCDMEKKDSTIQNYNFREYYEDNK
                     LKFDYILRKGVSQTRNARYLMKMAGIDIE"
     gene            2149..3894
                     /locus_tag="K8M77_000158"
     CDS             2149..3894
                     /locus_tag="K8M77_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBI7084689.1"
                     /translation="MFTEKVKAKEYYIKDLLSNKFLFEIPDYQRAYSWTKENLKQLVE
                     DIWESVELNKARGNKEFDQYEPYFLGSIVLCSKEYKDDGCGIYDVIDGQQRLTSIIML
                     IAAIRDLIDNEEYKKVLSDLIYQKPNVLMGIKESIRVKARGKEEEFFKKYILTNGGTE
                     LVKDLDMEELSEAKQNMVNAIEVFRDSFFNENGELLEEKLNEFIVYLLQKVVLVVITT
                     ESFTSAFRLFNVINARGLPLTDSDLLKSENLRVMNPEIRKEYTDIWESHEQDLGKEKL
                     DQIIGFMRTMKLKNKVEESVYEEFSKKIFRNEPEYLGVNFVNHLTAVKALYDKYIIDG
                     NLEGVSEEEKSYYKNLINIMREFLPYDDWMAAVIRFAEKFNDDKLVLEFVKVLEKRLV
                     IDWVNGNSFADRLNRVYGILEVIEEKDSLEEIKEAPVFLYDLERTTAYFENALNDIDF
                     YSKGRMMIPKYIFVRLDMEKRANEVLDYSDKIMIEHVLPRNAKEAYWKDNFSADQRRN
                     WANKFGNLVIITGAKNTRANNKPFAEKVEQYLSKKSDFAITKEVLELSDWNMDSLKDR
                     HESLVNRALELWTKF"
     gene            4149..5051
                     /locus_tag="K8M77_000159"
     CDS             4149..5051
                     /locus_tag="K8M77_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBI7084690.1"
                     /translation="MGYVIIDLEFNNMQNITKFYPNIYNEQKDLKELDSYNEIIEIGA
                     IKLNKFMQEVDEFKTYIKPSVFKVLNPKIIDITGITEDHLKDGIKFEDAMDNLKDFIG
                     EDDILCSWATDDIVHIVNNAKYHSYKNISWIKKYIDIQKYCTKILAHKKSLSLKHALE
                     ELKIKIDKSKLHDALNDAVYTAEVFKRLYNGKKVKEYIVEDVYNMPSIRVKDLNNYNL
                     ECEDIEFKCPKCNIKVEMDHPFRLFSWRFVSIGKCPKCNRNVRQEVVLKKTLSGNLVY
                     ESTNSLINDTEYIDLDYKFKKLAK"
     gene            5136..5546
                     /locus_tag="K8M77_000160"
     CDS             5136..5546
                     /locus_tag="K8M77_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBI7084691.1"
                     /translation="MNIAFFLTPKSEVAYEYTDATMRQVIERMEHHGYTAIPLIDKTG
                     KYVGTLTEGDLLWTLKRNPELNFKNTNTVSVKEIPRRVKHKSVCINENMESLISLAIS
                     QNFVPVVDDNGIFIGIIKRSDIINHCYNSLYKNN"
     gene            5712..6476
                     /gene="yaaA"
                     /locus_tag="K8M77_000161"
     CDS             5712..6476
                     /gene="yaaA"
                     /locus_tag="K8M77_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBI7084692.1"
                     /translation="MIALLSPAKTLDLTKPNLDIETSKPIFISEAEVIMNNLKELEIQ
                     DLCPLMKISEDLGVQTFTKIQDWNTIYYGDEKPFVLSFKGEAYRGLDADDFTKEDLEF
                     CNDSLRILSGLYGALKPLDGTKAYRLEMGTKISIDGSKNLYDFWGNKIMDSLLKDLEN
                     HKEKVIINLASNEYYKSIKQIDKKVRVITPVFKERKGIEYKVVTVYAKKARGQMVRYI
                     TKNRITKSEDLKNFDLYGYEFNERLSEGDTWVFTRD"
     gene            6581..6784
                     /locus_tag="K8M77_000162"
     CDS             6581..6784
                     /locus_tag="K8M77_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBI7084693.1"
                     /translation="MSKLILEIDGMSCSNCVRHVEAALLDVDGVDVLEVEVGRAVVDT
                     EVDEDTLRDALEEVGYELVSIED"
     gene            6930..8066
                     /gene="rpsA"
                     /locus_tag="K8M77_000163"
     CDS             6930..8066
                     /gene="rpsA"
                     /locus_tag="K8M77_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBI7084694.1"
                     /translation="MSENLTMSELMDSFELKHFHKGEIVKGKVISVKNDEIIVNIGHF
                     ADGVVPRNEISNDKNFDINSINVDDDIFVMVLSGDDGEGNVLLSKKRADAIKVWDDLK
                     EAFEKEKSIKVSLKEVVKGGIVGDFNGLRVFMPASQCAGRRIENLEELVGKTLEVRVI
                     EFNKENRKVVVSRRIIDEEIRNNEKKALWSSIKEGEKRKGKVTRLAKFGAFVDIGGVE
                     GLVHLSDMSWSRVHKPEEVVSVGDEVEVFVSEVDMDRERIALSLKDVIKNPWETLEGL
                     KVGDVVSGKVTNFIKVGAFVEVLPGIEGLVHISEITDENIAKPSDILELGQEVKVKIL
                     NIDDENKKMSLSIKDAVETSNEYIQYNDEEEGYSLADLFKGLNL"
     gene            complement(8180..9604)
                     /gene="pyk"
                     /locus_tag="K8M77_000164"
     CDS             complement(8180..9604)
                     /gene="pyk"
                     /locus_tag="K8M77_000164"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI7084695.1"
                     /translation="MQKTKMIFTIGPSSDSEEILREFIRIGMNAARLNFSHGDHASHK
                     EKIELIKRLRKEEKSATAILLDIKGPKIRTHNFKNGEAELKNGDEFTFSCGDEILGDN
                     TKCSISYKELYEDIKPGGSILVDDGLLEFKVKEVRRTDIICEVIEGGTIKDHKGVNVP
                     NVPIKLPAVTEKDRSDLIFGCEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIIS
                     KIESQEGVDNIKEIIKVTDGVMVARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITA
                     TQMLDSMIRNPRPTRAEASDVCNAIFDGTDAIMLSGESASGSFPIEAAMTMSRIAKKA
                     EANLDYNYLLRRLKDPNPNPDAFADAISYSASKTASKFPTKAIVAATQTGSTAKILSK
                     YKPSCPIIAITPYEKVRRSLALNFGIISKKCAYFNSTDEIIEEARKVAKEFQIAETGD
                     NIMVAAGFPTSITGGTNMLKIEKI"
     gene            9736..11187
                     /locus_tag="K8M77_000165"
     CDS             9736..11187
                     /locus_tag="K8M77_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBI7084696.1"
                     /translation="MNILKGLEPQSVLKYFEEISQISRGSGNEKGISDFLVNFGKNLG
                     LETIQDESLNVIIRKPATPGYENAPGVIIQGHMDMVCEKNKDTIHDFEKDPIKLRVDG
                     DYIYATGTTLGADNGIAVAYGMAVLASNDIAHPAIELLVTTDEEVGMGGAIALDGTLL
                     KGKYLLNIDSEEEGKLLVSCAGGARSEVTLPINFEEMEKDFEVYEIMLRGLKGGHSGM
                     EIDKQRGNSNKLMGRVLNDINANCDIRLISINGGSKVNAIPRECDTLLAVKKEDVKKL
                     EELIQKWDSILKDEYHANDSGVNVTLVKKEENHKVFSKDTTFKAIKIMNLIPDGVDTY
                     SIEMKGLVQSSTNLGVVTTEGDKIVFASSTRSSVETLKTKLLDEIADVAEILGGEFEI
                     QAPYPAWQYNPDSKIRELCSNVYKNMTGKDPEIIAIHAGLECGLLGEKIEGLDMISFG
                     PNMYDVHTPNEHVSISSVKNVWDFLVEILKAIK"
     gene            11485..12303
                     /locus_tag="K8M77_000166"
     CDS             11485..12303
                     /locus_tag="K8M77_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBI7084697.1"
                     /translation="MNLVRTDLHMHTRASDGTLTPKELLKEIVEKDIKLFSVTDHDSI
                     GSLEEMQDLTEGMDIKFIPGIEISSIINGEQFHILAYNFDKNNKELMSLIENNDRLLQ
                     EKDDNSIKQLIDAGYDIDFEDYLSYEHDPSKGGWKTLNFLIDRGICKNIDEFFNKIFV
                     GERALKYPDFPHPKEVIETIKKANGIVVLAHPKYGKSQFKLDEMLELFKNWGVDGIEC
                     YHPHHDEDTIKYLVDYCKKNNLIITGGSDYHGGLISKRSLGTPEFYAKYDLLDK"
     gene            12565..13368
                     /locus_tag="K8M77_000167"
     CDS             12565..13368
                     /locus_tag="K8M77_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBI7084698.1"
                     /translation="MKYTRIDVKNKKRSNGHNNNNNNDGKSFILLFIGVIVVALVIST
                     AMSKFIWPEGKKTAEKEKIEVTSGENKETNEKDKENNKVAKEDEKPKDNKVMAESVGG
                     QEYVMIQCGFYSNKENANNVKAQLEDTYIAVSLSEAENYRVIVHIGNEEEATKLSNEL
                     NEKGVSNTKGRFMIPKADVCSNEIIEIVNGYVNIINKLKEDSVKGVKTTEFKEWANRL
                     EEHSDSKYYKVFKELKDGINKIPDEITKKNIEESYQIVFNTLNNFKVNK"
     gene            13594..14172
                     /locus_tag="K8M77_000168"
     CDS             13594..14172
                     /locus_tag="K8M77_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HBI7084699.1"
                     /translation="MKVILASKSPRRVEILEKIVKEFEVVQSNFDENTIDFKGDVEKY
                     VKDLSRNKAIEVSKRLNEPSIVIAADTVVFQNGKVLEKPKSEEDAFSMLSSLSGNTHK
                     VYSGICLINTYDDTVVTDCDCTEVKFSELNPRQIRNYINSGEPMDKAGAYGIQGLGGA
                     FVERIEGCYYNVMGLPLNKLYKALENYDITIL"
     gene            14219..14902
                     /gene="radC"
                     /locus_tag="K8M77_000169"
     CDS             14219..14902
                     /gene="radC"
                     /locus_tag="K8M77_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBI7084700.1"
                     /translation="MSENIKINEIPSGERPREKLLFYGAQCLSNEELLAIILRTGNKD
                     SNVVELSYRIIHSVGGLNGLFKASAKELMELKGVKEAKATQILAMCELYKRFKVSELT
                     QIKISKPSDVAKLVLDELRMLRQEVLILINLDTKNKVISKKEIFKGGLNSSLVHPREI
                     FKEAVKDSAASIIICHNHPSGDPTPSRDDINITTRLKECGKMMGIELLDHLIIGDNRF
                     ISLKEKDIL"
     gene            14922..15950
                     /locus_tag="K8M77_000170"
     CDS             14922..15950
                     /locus_tag="K8M77_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBI7084701.1"
                     /translation="MGLFNFGMTKDMGIDLGTANTLVFVKGKGIVLREPSVVAINNLT
                     KKSLAVGTEAKQMIGRTPGNIVAIRPLKDGVIADFDVTQTMLKRFIEKITNKSAFTSP
                     RIIVCFPSGVTEVERRAIEEATKQAGAREVILMEEPMAAAIGAGLPVDEPTGSMIVDI
                     GGGTTEVAIISLGGIVTSKSLRVAGDELDQSIINYIKREFNLMIGERTAEQIKMEIGS
                     AYKVDENEEEQTMDIKGRDLISGLPKVVVVDAGQIRNALKEPVSSIIESIKTTLEKTP
                     PELAADIMDKGIMLAGGGAFLKGLDELIYKETGMPVHIAESPLDCVALGTGKALDKFD
                     LIVKQQRG"
     gene            15954..16805
                     /gene="mreC"
                     /locus_tag="K8M77_000171"
     CDS             15954..16805
                     /gene="mreC"
                     /locus_tag="K8M77_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBI7084702.1"
                     /translation="MKFFKNKLAVTVILLSVAFLGIIIFTVKSDSKNPISGGVGTVLN
                     PVQKVVYTISDKVHDVFDFFYNFSDVKNENNDLKKKNLELENQLVEYNELKRQNDVLR
                     GIVDFSKENNQYKYIGTNIIGKSGNSFVEGYTIDKGSSEGIKKDYIVISPEGLVGVVT
                     EVHSNWSKIQTLLNENVAVAATIEGDSNSGDGIVKGYRNGSEMQAEITNLPMNSTIKE
                     GETVVTSGLGGYYPKGIRIGEVTSVQSDDVKVMKTAILKTYVDFNNLQELFVVVPPSE
                     RDGIVKY"
     gene            16821..17318
                     /gene="mreD"
                     /locus_tag="K8M77_000172"
     CDS             16821..17318
                     /gene="mreD"
                     /locus_tag="K8M77_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBI7084703.1"
                     /translation="MKRLILILICLLLFIVDNTLVPFFSIKGVYPSLLFTFAVLYALM
                     SGYWEAVFIGVLSGFLQDVYFVNVFGVNMLVNMLVCLIAAYIGESVFKHKKTIPVLSV
                     GLLTIIKFFIVAFILNLINIRINLLSFWIMVLYNVVIGFFMYNWVYKLCNRDLMKREW
                     KISEK"
     gene            17327..20404
                     /locus_tag="K8M77_000173"
     CDS             17327..20404
                     /locus_tag="K8M77_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBI7084704.1"
                     /translation="MRSSINGKTPIDRYKILIGIMIIVFVSIVARLVYLQVFNYEEFK
                     QRADTRSMRFLSEQAPRGKIYDSNGDILATNRQTYTLTFTETDESKKNFYTTIDKVYS
                     ILKENNQSILDNFKIRINENGELYFDVSGDTEKLRLLKDIGMGDKVKNVLFKNKEGDL
                     TAEEEDQIDKELLKITPDEAFNFLVKEYKLYDVLNPTEEEVKSYKNMESKEITDILLK
                     KYSLEDIRGYMLVKNAMKMQSFSGYKPVTIAKDINKETAFIFYQRLNDLPGIDVSIEP
                     MRYYPYNELGSSFLGYVASINGYQKAKYEARGYDASTDLIGKAGIEAAFENELKGVKG
                     GTTVKVNSSGRKKEELYDLESYPGNNVHLTIDKDIQYSAETSMKYLLDYLQTRNTLDS
                     GVNTKNATRGAAVAIEVDTGKVLALVSYPGFNPNEFAKGELDPGVAKEYFQPDYEAFA
                     QQFIARNALNKTVDDLFPKNSKTGLREDLYDLYPKRFYNYATLGMIPPGSTFKPVTSV
                     AALEEGAITPSSTVYANGQNFGQPYPEIFGKNPANWPRDKNFSGYADLYTALQHSSNS
                     YYFDSAVKMYYKNGGDPYGDGRYKKSSTEALDSIAKYAWQFGLGVDPNSDKKASTGIE
                     ISENFGQVYNFESFKNQTIALMRFTLVDSLEKGTFVNSSSPDFPQVNIGVNDRDSKEL
                     AAAKESIKKDVENSLNKLGDGNTIDEKALSESLKKDFKTLYNLDSAYQESIKNFNGIK
                     GKDEDEKVDKAINLTVQRIVAWVVGDIAYQIVSPRNLTDSAIGQSISNFTPVQLASYV
                     ATLANGGTRYKTHLVDKITDAQGNLVEEFKPEVLNKVNMSKATLDAVKEGMRRVNEEV
                     GGSANFIMSKLPIVTGGKTGTATFRNDQSDYGRTDYGVYISFAPLDNPKIAVATVLYD
                     GGHGYYGSFIARTIYETYFREELKKDYPGYTPTNLDGATYDYSLNPPLEAIKDSNINS
                     KVENAELDSSGKPVEGSQDNNSENKKEDDKSQNNNQDNNNQNDKTKVDENKAH"
     gene            20651..21286
                     /gene="minC"
                     /locus_tag="K8M77_000174"
     CDS             20651..21286
                     /gene="minC"
                     /locus_tag="K8M77_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBI7084705.1"
                     /translation="MRDDRIFIKGNKLGINAIINMDKFGNFDEMLDSLVEKLSRGKKF
                     YKGATLTVTTDLKYINERQISKLKDVLFDEILIKDCIFEERLEKQSSVFSGVYEGRTK
                     FVRKTVRSGQCLNYAGNLIIIGDVNNGGEVRAHGNVIVLGDLKGKVFAGDNGNENAII
                     AAYSLEPELISISGKITISPDDFEKTGYPEVARLNENNIIVEPYLPDKYSY"
     gene            21315..22112
                     /gene="minD"
                     /locus_tag="K8M77_000175"
     CDS             21315..22112
                     /gene="minD"
                     /locus_tag="K8M77_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBI7084706.1"
                     /translation="MGVSIVITSGKGGVGKTTTTANIGTALAAQGKKVVVVDGDTGLR
                     NLDVLMGLENRIVYTVIDVIENRCRTKQALIRDKRFNNLYLLPTAQTKDKNDISPEQM
                     LRLVNELKEEFDYVILDCPAGIEQGFENAIVGADRAIVVVNPEITSVRDADRVIGKLD
                     AKGLENHEVIVNRLNYEMTKKGDMLDISDIIETLSVKLLGVVPDDRNITVSTNKGEPI
                     VLDEKASAGQAFRNIGRRIIGEDVPIMDLNTEHQGIFSSILKLFRRG"
     gene            22123..22395
                     /gene="minE"
                     /locus_tag="K8M77_000176"
     CDS             22123..22395
                     /gene="minE"
                     /locus_tag="K8M77_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBI7084707.1"
                     /translation="MSFLNVFSSRPTPKQVAKDRLKVILIHDRGELSDEVLDKIRLEI
                     LDVLSKYVEIENEDVDITVTKSNAIEGESPSLVANIPIKNIKGKAR"
     gene            22543..23667
                     /gene="rodA"
                     /locus_tag="K8M77_000177"
     CDS             22543..23667
                     /gene="rodA"
                     /locus_tag="K8M77_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBI7084708.1"
                     /translation="MLSRLKLDSKLRREIDYKLLVSMILIVLFGILNIYLGTKSQRGF
                     FFAKKQLIWFIISMAALYIILLWNYNIIYNYVEIFYWGSIVLLIITRFAGSVINGARG
                     WIVLGPVSIQPSELAKTAMILMLAKKMEQVDLRINDFRNFIKVAMYAMIPMIFIVVQP
                     DMGMTMVSFFIALGIFFAAGLDIKVIGAGLLSLIVAIALVWNSGIIKDYQKDRLVGFL
                     NPDGDELGINLQLTQSKIGIGSGGFFGTGLDLNGEVGGYSSEFVPERQTDFIFAVIGE
                     HWGTVGGIVLLLLYAIMIYRIIMTAKTSKDIFGSIICVGFASYFIFAILQNIGMTIGI
                     MPITGITLPLVSYGGSSLLTTIVSIALVLNISMRKKKLKF"
     gene            23819..24661
                     /locus_tag="K8M77_000178"
     CDS             23819..24661
                     /locus_tag="K8M77_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI7084709.1"
                     /translation="MGSYNKEYEKYYRKINKTRKTSPNHEANLKAKGSYKRKEKNSFG
                     KYLIKEVVLSSILGIFIFSSFFFMGQVENEFLVGLSRKFKEVISTDRYYKNLDLTESK
                     IVTTISSGIKDGFNLENSNLKKEPKQVSEEKVSENSGEVSAFNNFQIIEKESIDFLKE
                     SKVIPFNGEAKTLENNNLKEKNLYLSGSKGEVKSLLKGTISEVKNENGTYTVKMNYYD
                     DLEVLYHNLEEVLKKEGDSIEEGEKIGTSSENEKVKGVIIQVLFKNEYVNPEESMRFL
                     GDKQ"
     gene            24658..25512
                     /locus_tag="K8M77_000179"
     CDS             24658..25512
                     /locus_tag="K8M77_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBI7084710.1"
                     /translation="MKIDKKIIIQLVTFFILGFKFNLALAFFWIIIHELAHYFVLLKL
                     NIESENFKLHILGARLETKDYECLSSREKLMVCFAGPISNGIVFLIFFFIYKLIFKNE
                     YIYSTYEINLVLFIFNLLPTYPLDGGKILGAILEKKMIFKDVNNILIKISYSFGIAFI
                     LLSILGVLILGEINITSILAGIFIIYLSYNERRKVMYIIMGDITKKRERLINKKYIDS
                     RVTSVYCEQDMINLLRIIDKNRFNIFYVLDEEMNLLYVLRENEIIETLKTIGNINLRE
                     YYIKYRKE"
     gene            25644..27497
                     /locus_tag="K8M77_000180"
     CDS             25644..27497
                     /locus_tag="K8M77_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBI7084711.1"
                     /translation="MVRITDDILYRVEKPSRYVGGELNEVIKDPKEVDIRFGFCFPDV
                     YEVGMSHLGSRILYHVLNQRKDTYCERVYAPWPDMEKLMRENNIPLYALETKDPINEF
                     NFLAFTLQYEMSYTNILNMLNMGGIPLRASERTEKDPLVIAGGPCAYNPEPLYDIVDI
                     FQLGEGEEGLNDLLDLYQKHKDNFNKEAYLREASHIPSVYIPSLYDVTYNEDGTIKEF
                     IPKYDDVPKKVKKRIINNFDQVDFPESLIVPYSEIVHDRVVLELFRGCTNGCRFCQAG
                     MIYRPVREKSRKTLLDQARKMLKATGYDEVSLVSLSTCDYSDIQGLVKDLIEEHGKNN
                     VSVALPSIRVDAFSVDLLKEIQKVRKTGLTFAPEAGSQRMRDVINKGLSEERILEAAK
                     NAFESGWSTIKLYFILGVPYETVEDAAEIGLLAEKIADQYFAVPKHIRNKGLRITVST
                     SILVPKPFTPFQWAPMEKMDVVTEKINAVKGAIKSRSIVYNYHEQKTSYMEAVLARGD
                     RRLCDVLIKAYEKGAKFDGWSEYFDFELWQEALAECNVDGDFYVYRQRSYDEILPWDF
                     IDIGVTRKYLERENEKAKTGEPTQNCRKGCTGCGVNVNFKDGECFEGAILN"
     gene            27481..28182
                     /locus_tag="K8M77_000181"
     CDS             27481..28182
                     /locus_tag="K8M77_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBI7084712.1"
                     /translation="MRYLIKFTKDADIKFVSHLDLMRTIQRIVRRAELPVEYSKGFNP
                     HMALAIAQPLSVGVYSEGDYLDLNLTEDMNEETVKEKLNEFSANGIRFLEVSKSPVIH
                     NVKRLPAAMALLDAANYTIKIRYNDISKAEEEVNALMAKKEIITIKRTKKGEKEADIK
                     PFIKDFKCWTKDNYLIVNTTISCGSRENLSADLLANVIKENTSNVNEEAFVEIKRVEM
                     YAYKGDTLVPLYKYI"
     gene            28199..29641
                     /locus_tag="K8M77_000182"
     CDS             28199..29641
                     /locus_tag="K8M77_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBI7084713.1"
                     /translation="MRDIYVERRENLLRVAIKENNLLSECYIEEESLEPVQGEIYKGI
                     VKRVVPGIKSAFIDIGYSKQAYMILGEDEKDLKQGDGIIVEVLKEQFGSKGAKVTSKI
                     SLAGRYCVLETSRNDISISRKIENLEFKKELENSLIKPSELGVTVRTKAQSASIFEIQ
                     EEIEELYKNYKEVIREGKFSLNPKKIMGKGDLINKIIRDDIDAYTSSIIVDSIEDYER
                     IKDSISNSEYLKVTLYDDARSLFDYYGIEKEILSLRNNRVNLNCGGDIVIEKTEAMYV
                     IDVNSGKNTNHKNKEKTAYETNKNAAIEIARQIKIRNLGGIILVDFIDMYSQENKKKI
                     LKILKDEFKSDSQRAKIFPFTELGLVQITRYRKGKSIYEYIEENCLACNGHGKRLKLS
                     YINLLLRNEILKKNGEGKIKDFHIEINSIYEKDIRGNIFEFLKEIGALDLNIYLNFQE
                     SKDFYKVEPLIFKNQIESVKEYLVKNIEKY"
     gene            29767..30078
                     /gene="rplU"
                     /locus_tag="K8M77_000183"
     CDS             29767..30078
                     /gene="rplU"
                     /locus_tag="K8M77_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBI7084714.1"
                     /translation="MYAVLTTGGKQYRVQEGDVLFVEKLNAEVDSTVELTEVLAVAKD
                     GEIKVGAPVVEGAKVVAKVLAQGKAKKVVVFKYKRKKDYRRKNGHRQPYTKIVIEKIE
                     A"
     gene            30088..30459
                     /locus_tag="K8M77_000184"
     CDS             30088..30459
                     /locus_tag="K8M77_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBI7084715.1"
                     /translation="MIRVKFQSKEDNIISFTIKGHAMECRDAILAGEAYDMICNSVSV
                     LSQSAIIGLDEVLKLNVNYEMSDGYLKLDLNGFTHEEIVEAQVLLKTFEMSLASLVLG
                     LDSSLGKKTRCKYIEIIKEEV"
     gene            30463..30765
                     /gene="rpmA"
                     /locus_tag="K8M77_000185"
     CDS             30463..30765
                     /gene="rpmA"
                     /locus_tag="K8M77_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBI7084716.1"
                     /translation="MLKMNLQLFAHKKGVGSSKNGRDSEAKRLGVKCADGQFVLAGNI
                     LVRQRGTKIHPGANVGKGGDDTLFAKIDGVVKYERLGRDKKKASVYPVNVEEVIAE"
     gene            30933..32219
                     /gene="obgE"
                     /locus_tag="K8M77_000186"
     CDS             30933..32219
                     /gene="obgE"
                     /locus_tag="K8M77_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBI7084717.1"
                     /translation="MFIDTAKIFVKSGDGGHGSVSFRREKYVPLGGPDGGDGGKGGDV
                     TFVVDPGMTTLLDFKYKRKFVAGRGQDGQGSKCYGRDGENLTIKVPMGTIIRDVETNK
                     VMADLSHRDDTYTICRGGKGGKGNCKFCTPTRQAPTFAEPGMPGEERWVALELKLLAD
                     VGLLGFPNVGKSTLLSVVTKAKPKIANYHFTTLKPNLGVVAVPGIEPFVMADVPGIIE
                     GASEGVGLGLDFLRHIERTRLLIHVVDISGVEGRDAVEDFKRINEELKNYSVKLWDRP
                     QIVVANKCDMLFDEEIFENFKAEVNKMGFDKVFKMSAATSQGVEEVIKEAARMLKDIP
                     VTDLEIPEDERFIPEDKKFTYTINPIEEDGLKVYVVEGSFVDRLLLAVNVNDPDSLRY
                     FHKVLNNKGIFHELREMGIEDGDMVRLNDFEFEYLL"
     gene            32237..32539
                     /gene="yhbY"
                     /locus_tag="K8M77_000187"
     CDS             32237..32539
                     /gene="yhbY"
                     /locus_tag="K8M77_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBI7084718.1"
                     /translation="MITTKQRAYLRGLGAKLTPIFQIGKNGIEDNFLKQVDDALEARE
                     ILKINVLENSGLETREASNFICERLGCEGVQSIGNKIVLYRASKNNPKIELPKKGK"
     gene            32541..33149
                     /locus_tag="K8M77_000188"
     CDS             32541..33149
                     /locus_tag="K8M77_000188"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBI7084719.1"
                     /translation="MKKIGVFGGTFDPIHIGHIYIAYEAYKILELDEVIFMPAGNPPH
                     KKWKDITDEIIRYEMVKKAIEPYSFFSINNYEIEKKGLSFTYETLRYLHESFKEVELY
                     FITGADCLVNLNSWKNINEIFKFSNLVVFNRPGFDKNDLLKRKEEFDREYCTNIVYLD
                     LLNIEISSTLIRERVRQSLEVKFFLPPGVVDIIDKYNLYRRE"
     gene            33152..33721
                     /gene="yqeK"
                     /locus_tag="K8M77_000189"
     CDS             33152..33721
                     /gene="yqeK"
                     /locus_tag="K8M77_000189"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBI7084720.1"
                     /translation="MWSLEKINNYVKETIKGSRYSHTLGVVETAKELAKINGEDIQKA
                     ELAALIHDVAKYIPVDEQVKILEEHGYELDEITLKSPQVLHGFVGAIVAKEKFGIDDE
                     MVLDAVKYHTLAKKDMSTLEKIIYIADYIEPGRDFPGVEELREITREDLDKGVLKGLE
                     NTILFVIKQGNLIHHLTIEARNFLIMQEK"
     gene            33744..35057
                     /locus_tag="K8M77_000190"
     CDS             33744..35057
                     /locus_tag="K8M77_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBI7084721.1"
                     /translation="MGNEENRFEKRQDKKNINKKSLIKRIILGALAFILIFFISIFGY
                     FYFKYGSFLGEVFNIVKDVKQDVIKVEEPINVLLLGMDIGDTSDVSNKDAKRTDTIIL
                     ANFDPITKEAKLISIPRDTRVKIGGKSQKINAAYPIGGEKLVKQLVGNILGVKVDYVV
                     KVDYEGFRGIIDAIGGIDMYIEHDMNYDDPGQDLHIHFNKGETVHLDGKKAEEFFRWR
                     KNNDGTGLVNGDVDRIKNQQKFINAVIDKVLSVSTITKIDSIAEILNKNIETNIPLDV
                     TVAYGLKGLEADKNNIKMMTLPGEGKYVGNVSYYILDEKATKELKSQLNSSNLSGRKN
                     VIPSAARGDLSIKVLNCTKINGLAANVQKQLNENGYDKVDVGNGDPIEKSEILIKDES
                     SRAFMENDLKIDNIKKGISSKYDGNETYDVVILLGKDFKNFGEMK"
     gene            35054..35950
                     /locus_tag="K8M77_000191"
     CDS             35054..35950
                     /locus_tag="K8M77_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBI7084722.1"
                     /translation="MSILESKITKEYDKRKIREFLKEELGLSSRLIRSAAIDKRIKVD
                     GVAVKMNYVVKTGEEIKIELSKKESQNIEPEKIDIEIVYEDSDILVLNKKPFMVVHPT
                     RSHPNGTLANGILYYFKESNQDCIVRLVSRLDMNTSGLIIIGKNQYAHMVLSNEMKTD
                     KFQKRYLAIVHGNLEEKEGTINRPIYRPAEDTIKRVVDERGQESITHYKVIESYNGAD
                     LVECLLETGRTHQIRVHLSSLGHPIFGDSLYGTEDEEFISRQALHAYGLDFYSPKTKK
                     PLSLRADLPEDMKELINKLKNK"
     gene            complement(35982..37316)
                     /locus_tag="K8M77_000192"
     CDS             complement(35982..37316)
                     /locus_tag="K8M77_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBI7084723.1"
                     /translation="MSIKFKNLKIRLNFIISMAFIFSLILSTNVFAESNVPEIKGEGM
                     VLMDGTTGEVLAEKNANEKLAPASTTKTMTALLTVEKAKLNDVVTIGKNPPLAEGTRV
                     GLQEGDKYTVEELLNALLIQSANDAAVALAEHISGSEGEFAKLMNERAEELGAKNTHF
                     VNASGLFEDDHMTTPYDLALIMNAASKNPLIDEITKKTVYEMPKSIVSGEPIWANNNN
                     KLIHQTSPYYNKDIFCNKTGYTTKSRHTFTCAAKRGDRKLILTLLGYDTKDQYYEDTK
                     NLLDYGFNNFSLVKLYSKNDVIKEYYIDDKNNLSLLAPEDIYKTFKNSEIENLSTDEI
                     KKNFNIDFSVKATKPISRGADILKDTDSFTGILMIGNTPYKTLNLLNSNDITYTSFEK
                     IMFKLKGFSGFYIALGVIGLIFLIILILFMIRLISTIKRIRNRKKRNSYFYN"
     gene            37501..38265
                     /locus_tag="K8M77_000193"
     CDS             37501..38265
                     /locus_tag="K8M77_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HBI7084724.1"
                     /translation="MKRKEKIIGSLIISVIAILFLFIGYSKTNDKKISDSDMEKLFID
                     KEESEENNELEDFNEKESSSKKEESNNIKEEKYSDIESEKEGKKTSLDEEKIIVEIKG
                     EVKKPNVYELKNGSRIYELIEEAGGPTDEADLSNINRALYLSDGQCIVIKNINDVESE
                     EANLNEFLNAEVTNSIPTNSSGDKGEKNESSIININTASKETLMTLNGIGESKAQAII
                     DYRDEIGGFKSVDDITNVSGIGEKTLEKIKDKISIK"
     gene            38310..39341
                     /gene="selD"
                     /locus_tag="K8M77_000194"
     CDS             38310..39341
                     /gene="selD"
                     /locus_tag="K8M77_000194"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38352..38354,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBI7084725.1"
                     /translation="MIEKRLTELSKTSGUAAKIGPGALSEILSKLPKMNDKNLIVGID
                     TSDDAAVYKLNDEMATIQTLDFFTPIVDDPYTFGQIAAANSLSDVYAMGGKPIVALNI
                     ACFPNCLNMNILGEILRGGADKVLEAGAVIVGGHTVQDDEPKYGLSVTGIVHPDKVLK
                     NYGAETGDILILTKPIGLGIINTAIKAKIASKEAYEKAVKVMAYLNKYAGEIITDYNI
                     TSCTDITGFSLIGHAYEMAEPSKKTFRIFKDAIPFIKEAKEYASMGLIPAGCYENKRY
                     LEGKYLLKNVEGWMEDILFDPQTSGGLLISCKEKDYIDILTRLENLEVESAVIGRVED
                     FNDAYIVVE"
     gene            39358..40746
                     /locus_tag="K8M77_000195"
     CDS             39358..40746
                     /locus_tag="K8M77_000195"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBI7084726.1"
                     /translation="MTKELLRALPKIDEILGIFNEDFLNENGRETVVSALRDIINENR
                     KAILNEEVDYALTKEEAKSKCEHRLLKKRERNLKRVINGTGVVIHTNLGRSLLSKEAT
                     EAVALAASSYSNLEYDLEKGERGSRYSLIEGIIKDITGAEAALVVNNNAAAIMLVLNS
                     LCENKEVIVSRGELVEIGGSFRIPEVMNFSRAKLVEVGTTNRTHLYDYEDAITEETGA
                     FLKVHSSNFKIVGFTKSVSANEICNLAREKDIPVIEDIGSGVLIDLSKYGLEKEPTVI
                     ESLESGVDVVTFSGDKMLGGAQAGIIVGKKKFIDKIKRNQLTRALRVDKFTLAALEIT
                     LKHYLNEKEAIEKIPTLHMMTLDLKEIEERANRLYKNLEGLNKFYKFSIEEGESTVGG
                     GSMPDSKLSTYLLRIDSDSINEVNLERELREYKIPIITRVYKGAVYIDLRTILEDDYE
                     VIFNALKEIGEK"
     gene            40747..42654
                     /gene="selB"
                     /locus_tag="K8M77_000196"
     CDS             40747..42654
                     /gene="selB"
                     /locus_tag="K8M77_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBI7084727.1"
                     /translation="MKHVIIGTSGHIDHGKTTLIKALTGRETDKLDEEKKRGISINLG
                     FTFFDLPSGKRAGIIDVPGHEKFIKNMLAGATSLDVVLLIIALDEGIMPQTKEHLEIL
                     ELLEVKKCIVALTKRDLVDEEWAEMIKEDIKNYLKSTSFKDATMIDVSSKTKEGINEL
                     ITEIDSAVEEIEQKDKEGHFRLAVDRSFSVSGFGTVATGTILSGSVKLGDLVQINPSG
                     VEARVRNIQVHDENVEIGEAGQRCALNLSGVTKEEVTRGMVVCTSNTIEPSYMVDCKF
                     RYLKSNEKNLVNRQRVRIYHGTSEIFGRIVLLDKEEVKPGEEAYIQLRLESEICAQNG
                     DNLVIRNYSPMTTLGGGKIINPLAKKAKRFKEDYLNELKLMEKGSIEDIIENVVLSLS
                     ETFPNSQEVIKGLGRNIENIDFILEKLCEDKKIIGIDNGKSKVFIHNKFFTKKSEEIY
                     EILRRFHEENPLKVGMNKEELRSKSFSNKIKQKIFQSFLELMKEKEMIKEGINIISLK
                     DFEIKLTKAQNGIREKILKAYLESGIVSPKIKDIIEDKKLEKEYIKIYNLLIEDGTLI
                     KLPEDVVMHKEILQGVKDKIIEWLNKNGSITLGETKEILGVSRKYLVAILEYLDNEEI
                     TKRVEDKRVLN"
     gene            42841..43290
                     /locus_tag="K8M77_000197"
     CDS             42841..43290
                     /locus_tag="K8M77_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBI7084728.1"
                     /translation="MDDNKFAKEQENALKSDETSVTNEKMDSYEKEAAVLESISLDTN
                     TMAIDEEFMNKPDKDKKDINEKGEKNTSFFKRILEGIIDQTVVLLLSLAAFFIVDLIM
                     RPFGYKISLEGRIPFYFIIFVIMNILYRAIMEGRKFKLTLGEKVFRK"
     gene            43318..44676
                     /gene="rlmD"
                     /locus_tag="K8M77_000198"
     CDS             43318..44676
                     /gene="rlmD"
                     /locus_tag="K8M77_000198"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBI7084729.1"
                     /translation="MRKGNEIELLVEGIEFPAKGFGFVDGLKVYAKNTFPGQKVFGRV
                     TKKRKEYAEIRVSEVREKASYEEDQKCPHFGVCGGCSSQTIPYEKQLELKVEQVKDLF
                     KEAEVPTGEFLGIETSPIQWEYRNKMEYTFGDEEKGGELTLGMHMKGKSFGIVNVDKC
                     VLVDEDFRNILDTTVKYFREKGLPFYKIMPRTGYLRYLVVRKSVKTEEILVNLVTTTQ
                     IDFDLTEFTELIKGLNLKGKLKSIIHTESDALSEAVIPEKVNILYGDDFITENLLGLN
                     FKISPFSFFQTNSLGAEKLYSIVRDFMGAADNKVVFDLYCGTGTIGQIAAGKASKVIG
                     IELIEEAAMAANENAKLNGLNNCNFIAGDVAEVIKTVKDKPDIIILDPPRSGVHPKAL
                     DYVIKFNAPQIIYVSCNPKTLVTDLKVLEAKGYEIEKTLLMDMFPNTPHVETVVKLNK
                     IK"
     gene            complement(44902..45117)
                     /locus_tag="K8M77_000199"
                     /pseudo
     CDS             complement(44902..45117)
                     /locus_tag="K8M77_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591713.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            45694..46971
                     /locus_tag="K8M77_000200"
     CDS             45694..46971
                     /locus_tag="K8M77_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7084730.1"
                     /translation="MIKNAIAYITRKKNRTFIIFVILTIVFSCLYSCLSIMKSSASLE
                     KSLYKISNSSLSITKKDGGYFDINQFKDIEKIKEVEETIFQYNGLAKSIKAKVVGGEQ
                     KIERDNLPDEFKNILSLEATNKTKRNILFNSGVFTIKDGRNIEENDRRKILVHEDFAK
                     KNNLKLNDEISLELIEMENKVQKKEFKFEIIGIFSGKKQEQYTGLSSDFSENMVFVDY
                     ATSQEALNRAENNKIANKILIFSSSEESTNLALKKIKELKIDWSKHSIEKDTNAFEES
                     LESVSGIKHIIKIMTYSIMLGGIVVLSLILILWLRERIYEIGILLSIGISKIKIVIQF
                     ILELIFISLPSIMSTLVLGNLLLRQIVDGFIRSDNSMIVSNSLLNNSNSILNLGTLTQ
                     SYFILISIIILSVIVASSMILVKKPKEILSKIS"
     gene            46981..47637
                     /locus_tag="K8M77_000201"
     CDS             46981..47637
                     /locus_tag="K8M77_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7084731.1"
                     /translation="MDILEIKNLSYSYTNSREEILSSVNQKFELGKFYAIIGKSGTGK
                     STLLSLLAGLDKPQTGQVLFKNENIDKKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKKASENILIELGLDEKQIKRNVMKLSGGQQQRVAIARALVSKAPIILADEPTGNL
                     DSVTAGEIIDILKTLAKERNKCVIVVTHSKEVAEAADIILELSGKKLKKVSKINSEVQ
                     "
     gene            47637..49013
                     /locus_tag="K8M77_000202"
     CDS             47637..49013
                     /locus_tag="K8M77_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7084732.1"
                     /translation="MIKNAFAYVTRKSLKSVIILLVVLAMSALSLISLSIKDATNRAS
                     EETFGNITSSFSMEINRQVNPGTPRGGGNVKGEDIKKISENKNIYSYVKRINSVADLI
                     DHDIVETKETLANQSPERSKNFKRTVMLTGVNESSKENKFVSGAYKLIEGKHLENQDK
                     NKVLMHKDLAKKNNLKVGDKIKIKSNLFDADNEKGANETVEVEIKGLFDGHNNGVVSV
                     PQELYENTLITDINTAAKVYGNTEDTAAYQDATFFVKGDKNLEHVIKDIGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSIANKLFAGSLIFAGVVVSLLLFLWINARKKEIAVLL
                     SLGISKLTIFGQFLIELIFISIPAFIGSYFLASYTGDKLGNNILNRVTGDIAKKIAKQ
                     SLSSQLGGGAEVDGFNKTLTSLDINILPKSMVYVILFMSLVLIISLIISSYSILKKNP
                     KELLIDND"
     gene            49153..49806
                     /locus_tag="K8M77_000203"
     CDS             49153..49806
                     /locus_tag="K8M77_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7084733.1"
                     /translation="MKILIVEDDKIIREGISEYLSEFGYIIIEAKDGREALSKFNNDI
                     NLIILDIQIPYINGLDVLKEIRKKSKLPILILTAFSDEEYKIDAFTNFADGYVEKPFS
                     LPVLKARIDSLIKKNFGQIEKFEYKNIDVNFNSYTVKINGENIYIYAKELEILKYLVD
                     NEGQVLTRMQIIDNVWKETEEIPYDRVIDVYIKELRKKLKLDCITTIRNVGYKLERK"
     gene            49803..51122
                     /locus_tag="K8M77_000204"
     CDS             49803..51122
                     /locus_tag="K8M77_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7084734.1"
                     /translation="MKDLKIFPKIFIQTFSVIGIIIILMHLLIFLIFPRTYLDTRKAE
                     IYNKANEISRNMNGKELNYIEQTLDFYSNTGDIKAFIKDVNDNNEVKIKKQVNINLES
                     NNNSLIIEEREITINNGKKIYLQFVSIADMQKDAKNLSLKFLPYSLLISLLFSSIISL
                     AYAKSIKNNIQEIKNVTDKMMKLDKKARLKLDSNDEVGQLKEQINDLYTTLLRTIDDL
                     KFKNKEILKLEKLKYNFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECID
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDVLEGVLKKYEVLVNQKNITINNYILD
                     ENIYIGKTALNIILSNLISNAVKYTDANGIINIGLDRDWLYIENSYGDNKVSDIDKLF
                     EFKFDLNKENSNGLGLYIVSNLLNNYNKKFKVLQNREYFVFKIKLSS"
     gene            complement(51438..52805)
                     /locus_tag="K8M77_000205"
     CDS             complement(51438..52805)
                     /locus_tag="K8M77_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBI7084735.1"
                     /translation="MDINFEKLFLLENLTNHIPLGLIFISPENKIDFINKKAESFLNS
                     KSKSVENIDIFSFFPQLNELINNSSSKKNNQKDALVTSLDGNKYNLDICPINDGDIFV
                     GWLLILKDLKEIISTVNKYSNQEAAYTFDDIIGESPQLKKVLRDAELISKSPSTVLIT
                     GESGCGKELLAQSIHNASNVSNGPFIALNCGAIPKTLIESELFGYEEASFTGSRKGGK
                     PGKFELADGGTIFLDEICEMPIEMQVTLLRVIQEGVVTRIGGKTQIPINVRIIAATNK
                     NLKEEIKKGTFREDLYYRLNVMPLHLPPLKDRLGDVPILINYFLNIKSQKLHKGIPNI
                     NQKLYKKMISYCWPGNIRELENCVENIVNLNGSTSYEINFDECHCLKTDNLGNPINPN
                     SLDLPESYNENSIKPLIILEKEAIEKALCTFDGNITKASNALGISRNALYNKIKRYGI
                     NKYNY"
     gene            53212..54222
                     /locus_tag="K8M77_000206"
     CDS             53212..54222
                     /locus_tag="K8M77_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI7084736.1"
                     /translation="MEVNKRKFQKGLMFGILVGLAWGLDGVLMGRVGMDKIFTDKAFA
                     LAQGVSEISFEFSPLVTAFFHESFCFVWVALVLIFGKQFKHVFHLLFKTKKGKAAALA
                     ALVGSPIGMSAYLLGIKYATAPYASSISVIYPGVGAILSYFILKEKLSLRAVIGIAVS
                     LLGSFMLGFNPSGNVPPTFFKGILFAVVAVFGWALEGVIIGFAMKHIKGEDHIQASPQ
                     QFLCLRYFISMVSYAVIVLPFIGGYPLAGQIVSRGLVFKYAGIAILGAITYLSWYKAV
                     DLIGAAMGTALNSTAALWTIIFSALLFGDKITPSLAIWGIVIVLGVFIFAIDPKTLRK
                     KA"
     gene            54396..57008
                     /locus_tag="K8M77_000207"
     CDS             54396..57008
                     /locus_tag="K8M77_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003408617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7084737.1"
                     /translation="MLKKEIVSNMEKIVENCMGEETAACVATCPMHTDVKEYVRLIKE
                     EKGEEAIKVIREKLFIPGTLGRICAHPCEKQCKWNEGKSPMAIASLKRYAADNFDKEE
                     NWDLSIKNSNGKKIAVIGAGPSGLQSALDLRKEGFEVTVFEKLPVRGGMMRVGIPAYR
                     LPRTGLEHEISYLDKLGVNFELNCEIGKDKEFNDIVNEFDSVVVAVGKHQGRVDRSLE
                     NFDAEGIFSAAGYLKEASLTGNVEKAGKVVLVVGGGDVAMDCARTSLRIDGVEKVYSI
                     CLEDSFDNMASSNHEIEGAIAEGIKFNHAQAIKNIHIDENGRVSRVTLKKCLSMFDEN
                     GRFAPKFDDEATSEIEVDTIVFAIGQGVEGEFAKDILVQRPNTTFECDKETLQSSSNE
                     KVFIVGDASGESVIVIQAMATGRRAAQSVIRFLNGESLTEGRELKDTWTYETKLQMPT
                     DWSSITGKREDMEELDPAERIKSFDEVALGYTKEEALREADRCRQCQCKLCMKECIML
                     NDYTDCPKALFREYLEKGYENMDKMIAYSCNECNQCTLKCPKELDLKVNFRAMKEAFS
                     EENGGLAPLEALKASDATQEKECAEEYCTTVEAASVEEVKEKKKAKKKTKYVFVPGCT
                     VPAYTPGGVESVLRHLKDSLGEENVGALLQCCGKVTYLIGEEEKYEERNKKAIDILDE
                     MGAEVIITVCPSCYKVFKETAKNQRVIAYWDLMKYLIGIPAESKGIGEGSDVIFNIHD
                     SCVTRDVTSHHESVRWILDQLGYNWEEVERNGKNTRCCGVGGMVCSSNPELYERVYTR
                     RANDFNQDNIVTYCGSCRGTMQASGKDAVHILDLIFGSKYTKDQAQQRGYRTEEEMWA
                     NRLETKERLNKFKK"
     gene            57466..59076
                     /locus_tag="K8M77_000208"
     CDS             57466..59076
                     /locus_tag="K8M77_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBI7084738.1"
                     /translation="MPYVLQEVSPQETDNISTIVNNPYLDLDGNGVIVGIVDTGIDYL
                     NKEFMREDDTSRILTIWDQKSTKKPNDSVYVGSIFNNEDINNAIKSKADGRDPYDIVD
                     SRDEIWHGTKLASIIGARGYNRKIKGIAPNCDFAIVKLLNSFSYEKAFRENGIENVPV
                     YDEVEIVAGVEYLKNYALSLKRPLVICLAIGCTEASHDGRGLFPRYLTTVASIRGIAI
                     VAGVGNEGSAQGHASGVIELENSVEKIELSIPREIKNFNLAFWIQRPNIMSLNIKSPS
                     GEESSFIDAKIFLERSFKFILTDTSVNINYYVPDTFTGNELIYVEFKDIKPGIWTFEL
                     RGDYITNGRYDVWLAPSSLLPENTKFLSPNPLNTLMDPSTAKYIITVAYYNSQTQSLL
                     AESGKGFNVNGWINPDITTAGKDILTIFPGDRVGRMSGSSPATAITVGVCALLFQWGI
                     INRNDRTMNSSKLRSYLIYGATRIPGQTYPNEYTGYGYLDLYEIFRNIIGVPLPPYRS
                     TKINEDYTEYYCGRMLVSVPSDFYYGGK"
     gene            59078..60832
                     /locus_tag="K8M77_000209"
     CDS             59078..60832
                     /locus_tag="K8M77_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBI7084739.1"
                     /translation="MEFKSSAPEYVFNNENYLNYLVQYQGDIIGEFNNQNGIYATLIN
                     DRYAIITIDKNLQEYENDISMIKYKKENGRDVEIDNITYIKDPEGYVLQEISPLQAAN
                     IEYVQIQSYFNLTGKGVIVGILDTGIDYLNEEFMDSYGNTRILGIWDQTISSESSREN
                     NLPYGTFYSEEDINRAIRLSREGGDPYTIVPSRDEIGHGTSMAGIIGSSGKNPRLKGV
                     APDCKFLVVKLAQSLYYKKEYEIKIPVYNITEIFTGIQYLYSYFLKRSQSMIIYLPLG
                     TNRGSHKGTSILEEFLDSILINRGIALITGAGNEGAALLHGSGTIKPNGQVTTHEFNI
                     DENQKKIIIEAWIQIPSIASVEIVSPTGGTTGIIQPFFGKGNKYYFTIERTTVLVSYY
                     IPEEIYEDSLILIILDNVQAGIWSFKFRGLNNIEGRYNIWLPPKGISKEKTRMIYPDP
                     YGTVTVPGTSISVITVAAYNQLNNTQLIYSGRGFKDNYIDIIDVAAGGVNALTVAPDN
                     KTTLANGTSVAAAIVAGICVLLFQWGIVEGNYPYMFSQTLKAFITRGTRKRKGDTYPN
                     PEWGYGIVDMFNMFNLTN"
     gene            complement(60958..62379)
                     /locus_tag="K8M77_000210"
     CDS             complement(60958..62379)
                     /locus_tag="K8M77_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7084740.1"
                     /translation="MEAGLVNLITSKDENWDKRWKYNCAMFILCYIFMGAVTGITNDS
                     YLSYLNITVPNVVKALPTYASIGTFIIAMLLLLTHKVGFKKLILLAPIFSILGLLVCI
                     YSKDGLIITFAYIVVNIGLGMFDCIYPVMFTSYTPRKERTKMFSRVMYCNLISQSILT
                     FLNGKIVVWKFAKSLGISYNRASVLSGNQDALSSVQLMAYSDSYKFVLWIAIALTVIA
                     SVFLLFLKEKSEDYRETPEEIQARKGEKVFDFKLLANKYVILWILIFGIVRFGALLVT
                     PFFPIYLNNFLHISRGTVSSIITFQTISMVIGYFCTPYLEKKFGSIVTISVTTILCVP
                     LMILMANGAIFGSNVAMIVGIILFLRSGVANVSAPIQSSLPLTFVPKNLVSAYNSLIL
                     VVNSLIGIVAGIYTRYSLLKTESGYGKAYYIAGALYLIASIFLLIIFTKKYNRTNNDS
                     EVIEINAETAISSVDDSIKETLK"
     gene            62668..63117
                     /locus_tag="K8M77_000211"
     CDS             62668..63117
                     /locus_tag="K8M77_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7084741.1"
                     /translation="MIKSIDNIELKKELAYNLMNDFKNELYNKDIIERIINKIEQIPF
                     YVYIEKGNEIGFLSMKSLTEESADIYFIKMLDEYKEKGFEGELINNVATFYKEKGYKL
                     LRVKILADGKHDNLINFYRSVGFFKLEERIESGDIENKVLVMIRPLI"
BASE COUNT        25190 a         6543 c        11313 g        20111 t
ORIGIN      
        1 tattttaatt ttaaagctta atttaagtat atttttaatt tttatataga ctttatttat
       61 aagagattta aatcattttt tcatataaat ttagaaagtg tcatagttta aaggagagtc
      121 ttgcattaaa atatgttaac tatggtataa tattaaaaaa gtgttttgag aggataaata
      181 tggagaatag aaaagatatt tatgaaaaaa gaatagaaga gtattcaagt gctttaaaga
      241 gattaaagag agactataat ataatcagcg cattaagatt agtagtttct ttaagtattt
      301 tattttttat ttactacgct tatagcatag gctcaataag ctttgggatc atattatttt
      361 tgctaaattc tatattattt ttatatttag caaaggttca tgaaggtata gtgaataaaa
      421 taagtaaaag agaagctctt atagaggtta ataaaaaaga gattcttagg ttagagggaa
      481 agtggagaga atttaatgat ttaggagaag aatatttaga taataagcat ccttttataa
      541 atgacttaga tatatttggg aaaaactctt tatttcaatg gataaatgaa actggtactg
      601 tttatggaag agagaaatta agccatttat taaagttaga agaacttcca aataaagaag
      661 aaattttatt aagacaggaa gctttaaagg agctttctaa aaaagtagat tttagacatg
      721 aatttatagc ttcattaaaa gataaaaaag gaaaaaaaga gaagtattta ggggaatggt
      781 taaaagaaga cagtaaagcc atatcgcctt tattaaatat tcttagaata ataatgccag
      841 taataaatat tggaattact attttagttg gtatgaatgt tatttcatgg caaatactat
      901 taatttctct tgttataagt tatggtattt tgaagcttgg caataaggaa gttattaaag
      961 gattaaatat atttgaagat ttaaaatata gaataaaaac ctatgtagag gctttagagt
     1021 taatagaaaa agagaatttc cagtctaata taataaaaag tataaaaagt aacttagata
     1081 tgaatggcaa aagtgctagt aaggagctta aaagcttaga aaagataact agctggcttt
     1141 atgatagggg aaatgccttt tatcttttat taaactgtta tttgctttgg gattatcaaa
     1201 ttctatcaaa gcttgaaaag tggaagagtt ctaataaaga tgagttttat aaatggatga
     1261 tttctttagg tgattttgag gctttagttt ctttagctgg atttacttac aataatcatg
     1321 gatgggctac accaaaaata aatgatgact atactttaaa gggaaaaaat cttagccatc
     1381 ctatgttagg agaaaaaggc gttggaaaca gttttgatat taataaggat aagagagtaa
     1441 tcttaataac aggatctaat atgtcaggta agagtacatt tttaagaact gttggattta
     1501 attgtatatt agcttatcta ggacttcctg taaaaggaga aagttttgaa gctccaatat
     1561 taaaagttta tacctgtatg agaactggag ataatcttga agagagtata tcttcatttt
     1621 atgcagagat acttagaata aagattatag ttgagggtgt aaaaagagga gaaaagattt
     1681 tatttttgtt agatgaaata tttaaaggaa caaactcctt agatagacat gagggagcgg
     1741 agatattaat aaatcagctt ttagaaggaa acacattagg attagtttca actcatgatt
     1801 ttgaactttg cgatatggag aaaaaagatt ctactataca aaattataat tttagagaat
     1861 attatgagga taataaatta aagtttgatt atattttaag aaaaggtgtt tcacaaacaa
     1921 gaaatgctag atatttaatg aagatggctg gaatagatat tgaataaaag tatacttcta
     1981 tatataaaca gcaaaataac ttaattttag acaaattaaa ttaattttga tataataaac
     2041 aaatgatgta tttagtgaaa aagccttaaa tggctttttt tcatgagtag agttattagt
     2101 aaaagaggac ttagaataaa tagcaaaaaa ttaggaggca tgtatatttt gtttacagaa
     2161 aaggtaaaag caaaagaata ttacataaag gatttattaa gtaataagtt cttatttgaa
     2221 atcccagatt atcaaagagc atattcttgg actaaagaaa atttaaagca attagttgag
     2281 gatatttggg agtcagtaga actaaataaa gcaagaggaa ataaagaatt tgatcaatat
     2341 gagccatatt tcttaggaag tattgtttta tgttctaagg aatataaaga tgatggctgt
     2401 ggaatatatg atgtaataga tggacaacaa agacttacat ctataatcat gttaattgct
     2461 gcaataagag atttaataga taatgaagaa tataaaaaag tattatctga tttaatatat
     2521 caaaaaccaa atgttcttat gggaattaaa gagagcataa gagttaaggc tagaggaaaa
     2581 gaagaagagt tctttaaaaa gtacatatta actaatggtg gaacagaatt agttaaagat
     2641 ttagacatgg aagaactttc agaagctaaa caaaatatgg ttaatgcaat agaagtattt
     2701 agagatagtt tctttaatga aaatggagaa cttttagaag aaaagttaaa tgagtttata
     2761 gtttacttac ttcaaaaagt tgtactagtt gtaataacaa cagaatcatt tacttcagca
     2821 tttagattat tcaatgtaat aaatgcaaga ggattacctt taacagattc agatctttta
     2881 aagagtgaaa acttaagagt tatgaaccct gaaataagaa aagagtacac tgatatttgg
     2941 gaaagtcatg aacaagattt aggtaaggaa aaactagatc aaatcatagg tttcatgaga
     3001 acaatgaagc ttaaaaataa agttgaagaa agcgtatatg aagagtttag caaaaagata
     3061 tttagaaatg aaccagaata tctaggagtt aactttgtaa atcatttaac tgctgtaaaa
     3121 gctttatatg acaagtatat aatagatggt aatcttgaag gtgtatcaga ggaagaaaaa
     3181 tcttattata aaaacttaat aaacataatg agagaattct taccatatga tgattggatg
     3241 gcagcagtta ttagatttgc tgagaaattc aatgatgata agttagtttt agaatttgtt
     3301 aaagttctag aaaaaagact tgttattgac tgggtaaacg gaaattcatt tgctgataga
     3361 ttaaatagag tttatggaat tttagaagtt atagaagaaa aagattcttt agaagaaata
     3421 aaagaagctc cagtattctt atatgactta gagagaacaa ctgcatactt tgaaaatgct
     3481 ttaaatgata tagatttcta tagcaaaggt agaatgatga tacctaagta tatcttcgta
     3541 agacttgata tggaaaagag agctaatgaa gttttagatt atagcgataa gattatgata
     3601 gagcatgttc ttcctagaaa tgctaaagaa gcttattgga aagataactt ctcagctgat
     3661 caaagaagaa actgggctaa taagtttggt aacttagtta taataacagg tgctaaaaat
     3721 actagagcta acaataaacc atttgctgaa aaagttgaac aatacttaag taagaaaagt
     3781 gatttcgcta taactaaaga agttttagaa ttatctgatt ggaacatgga ttcattaaaa
     3841 gatagacatg aaagcttagt taatagagca ttagaacttt ggactaagtt ttagaaatta
     3901 aaagggcatg tatcaaaata acttttattg ttatattttg atacagcccc cttttttatt
     3961 taaataaaag ttaagttttg tctattgaaa attaggaatt aactcaataa aataaaagtt
     4021 attagatagg ataattaaga tttattatta gaaaaagtag cagataaata ataaatcaat
     4081 tttaatgaaa aaatccaaaa ttcatggcat aattatatat ggatatagat agtgagagga
     4141 tgtagagaat ggggtatgta ataatagacc tagaatttaa taatatgcaa aatattacaa
     4201 aattttatcc taatatttat aatgagcaga aagatttaaa ggaattagat tcctataatg
     4261 aaataataga gattggggct ataaaattaa ataagttcat gcaagaagta gatgagttta
     4321 agacatatat taaaccatca gtatttaagg ttttaaatcc aaagataata gatataacag
     4381 gaataactga agatcattta aaagatggaa ttaaatttga agatgccatg gacaacctta
     4441 aagattttat tggggaagat gatatacttt gttcatgggc cactgatgat attgttcata
     4501 tagttaataa tgctaagtat cattcataca aaaatataag ttggataaaa aaatatattg
     4561 atatacaaaa atattgcaca aagatattag cgcataaaaa atctttaagc ttaaaacatg
     4621 ctttagaaga gttaaagata aaaattgata aaagtaaact acatgatgca ctaaatgatg
     4681 ctgtttatac ggcagaggtt tttaaaagat tatataatgg taaaaaggta aaagaatata
     4741 tagtagaaga tgtttacaat atgccttcta taagggttaa agatttaaat aattataatt
     4801 tagaatgtga agatatagaa tttaaatgcc ctaaatgtaa tattaaagtt gaaatggacc
     4861 atccttttag attatttagt tggagatttg tatcaatagg aaaatgtcca aaatgcaata
     4921 gaaatgttcg acaagaggtt gtactaaaaa agaccttatc aggcaattta gtttatgaat
     4981 ctacaaactc tttaataaat gacacagaat acattgatct tgactataaa tttaaaaagt
     5041 tagctaaatg acaatagatt gtcacttaaa aaacatatat atgataaaat aaaagtataa
     5101 caattactat aaaaaattag gggaactgat atattatgaa tattgctttt tttctaacac
     5161 caaaaagtga agtagcatat gaatatactg atgcaacaat gagacaagtt atcgaaagaa
     5221 tggagcatca tggatataca gctattccac ttatagataa aacaggaaaa tatgtgggta
     5281 cattaacaga aggggactta ctttggactt taaaaagaaa tccagagctt aattttaaaa
     5341 atactaacac agttagtgta aaggaaatac caagaagagt taaacacaaa agtgtttgta
     5401 ttaatgaaaa tatggaaagt ttaatatcat tagcaatctc tcaaaacttt gtaccagttg
     5461 ttgatgataa tggaatattt ataggaataa taaaaagaag tgatataata aatcactgtt
     5521 ataatagtct atataaaaat aattaaggtc ttttggcctt aatttttttt ataaatatat
     5581 tgattgaatc ttctgaaagt attattatta attatattta aataattcta taataatgta
     5641 tttagggtag ttttaaataa aattatttta ttatataatg tttttaacaa aattagaaag
     5701 aggtgatact tgtgatagct ttattatcac ctgcaaaaac tttagattta acaaaaccaa
     5761 atttagatat agaaacaagc aaacctattt ttatcagtga ggctgaagta atcatgaata
     5821 atttaaagga attagaaatt caagatttat gcccccttat gaaaataagt gaagatttag
     5881 gagtacaaac attcaccaaa attcaagatt ggaatacaat ttactatggt gatgaaaaac
     5941 cctttgtatt atcttttaaa ggggaagcct atagagggct agatgcagat gattttacta
     6001 aagaagattt agaattttgt aatgactctt taagaatatt atcaggactt tatggggcat
     6061 taaagccttt agatggaaca aaggcatata gattagaaat gggaacaaaa atttcaattg
     6121 atggctctaa aaatctttat gacttttggg gaaataaaat aatggactct cttttaaaag
     6181 atttagaaaa tcacaaggaa aaagttatta taaatttagc ttctaatgag tattataagt
     6241 ctataaagca aattgataaa aaagttagag ttataacccc tgtatttaaa gaaagaaaag
     6301 gtattgaata taaggttgtt acggtgtatg ctaagaaggc aagaggacaa atggttagat
     6361 acataacgaa gaataggatt actaaatcag aggatttaaa aaactttgat ttatatggat
     6421 atgagtttaa tgagaggctt tctgaaggag atacatgggt atttactaga gattagctaa
     6481 aaatcataga tattaaaaac ttaagggtta atatttatga attattatat aaaattaaaa
     6541 taaataatat aaattaaaaa tttaaggaga gatttttttt atgagcaaac ttattttaga
     6601 gattgatgga atgtcatgtt caaattgtgt tagacacgtt gaagcagctt tattagatgt
     6661 tgacggagta gatgttttag aagtagaagt tggcagagct gttgttgata ctgaagttga
     6721 tgaagatact ttaagagatg ctttagaaga agttggttat gaattagttt caattgaaga
     6781 ttaaattgtt taattaccta atggcaatat gttattaggt aattttttat ttatataaaa
     6841 ctacaataag ctatatgcct acttgtttta taacatatag ttatgtaaaa tattttataa
     6901 agtaagcctt aaataaagga gatgtaaata tgagtgaaaa tcttacaatg tcagaattaa
     6961 tggatagctt tgagttaaag cattttcata aaggtgaaat agttaaaggg aaagttataa
     7021 gcgtaaagaa tgatgaaata atagttaaca ttggtcattt tgcagatggt gtggtaccaa
     7081 gaaatgaaat ttcaaatgat aagaattttg atataaattc aataaatgta gatgatgata
     7141 tatttgtaat ggtattaagt ggagatgatg gagaaggaaa tgttcttcta tcaaagaaaa
     7201 gagcagatgc aataaaggtt tgggatgatt taaaagaagc atttgaaaaa gaaaaatcaa
     7261 taaaagtttc cttaaaagaa gttgttaaag gtggaatagt aggagatttt aatggtctta
     7321 gagtatttat gccagcttct caatgtgcag gaagaagaat agagaattta gaagaattag
     7381 taggaaaaac tttagaagtt agagttatag aatttaataa agagaataga aaagttgttg
     7441 tttcaagaag aataatagat gaagaaataa gaaataatga aaagaaagct ctttggtctt
     7501 caattaaaga aggagaaaaa agaaagggaa aagtaactag gttagctaag tttggtgctt
     7561 ttgttgatat tggtggagta gaaggattag ttcacttaag tgatatgtca tggtcaagag
     7621 ttcataagcc agaagaggtt gtatcagttg gagatgaagt agaagtattt gtatcagaag
     7681 tagatatgga tagagagaga atagcacttt cattaaaaga tgttattaaa aatccatggg
     7741 aaacattaga aggattaaaa gttggtgatg ttgtttcagg aaaagtaact aattttataa
     7801 aggttggagc ttttgttgaa gtattaccag gaatagaagg attagttcat atttcagaaa
     7861 ttactgatga aaatatagct aagccatcag atattttaga attaggccaa gaagttaagg
     7921 ttaaaatctt aaatatagat gatgaaaaca agaaaatgag cttaagtata aaagatgctg
     7981 tagaaacttc aaatgaatat atacagtata atgatgaaga ggaaggttat tcattggctg
     8041 atctattcaa aggattaaat ctatagttct taaaaataaa aactatagat ttaattaaat
     8101 aatagatttc attaagaatt tataggcttt atagattaga aaaggatagt acttttagta
     8161 ctatcctttt tatattaaat tatatttttt caatttttag catgtttgtt ccaccagtta
     8221 ttgatgttgg gaatccagca gctaccatta tattatcgcc agtttcagct atttggaatt
     8281 ccttagctac ttttctagct tcctctataa tttcatctgt gctattaaaa taagcacatt
     8341 tcttagaaat tattccaaag tttaaagcta agcttcttct aactttttca tatggagtaa
     8401 tagctattat tggacagcta ggtttatatt ttgataatat cttagcagtt gaaccagtct
     8461 gagtggctgc tactatagct tttgtaggga atttgctagc tgtttttgat gctgaataac
     8521 ttatggcatc tgcaaaggca tctggatttg gatttggatc ttttaatctt cttaaaagat
     8581 aattatagtc taagttagct tctgcttttt ttgctattct agacattgtc attgctgcct
     8641 ctatagggaa gctaccagaa gcactttctc cactaagcat tatggcatct gtaccatcaa
     8701 atatggcatt acatacgtca gaagcttcag ctctagtagg tcttggattt cttatcatag
     8761 aatctaacat ttgagttgca gttataacta tttttcctgc ttgattacat ttcttaatta
     8821 tatttttttg aattataggt acattttcta taggaatttc tactcccata tcacctctag
     8881 caaccattac accatcagta accttaatta tttcttttat gttgtctaca ccttcttggc
     8941 tttctatttt tgatattatt tttatatctt ttccaccatg agagtctaga atttctctaa
     9001 cttcaagtac atcctcaggt tttcttatga aagaagctgc tacaaagtct acttccattt
     9061 cgcaaccaaa tattaagtca gatctatcct tttcagttac tgcagggagt ttgattggta
     9121 cattaggtac atttacacct ttatggtctt ttatagtacc tccttcaata acttcacata
     9181 ttatatcagt tctacggact tcctttactt taaattctaa aagtccatca tcaacaagta
     9241 ttgatccacc aggttttatg tcttcatata gttctttata agagattgaa catttagtgt
     9301 tatcacctaa tatttcatct ccgcaagaga atgtgaattc atcaccattt tttaattcag
     9361 cttcgccatt tttaaaatta tgtgttctta ttttaggacc cttaatgtct aataagatag
     9421 ctgtagctga tttttcttct tttctaagtc ttttaataag ttctatcttt tctttatgac
     9481 ttgcatggtc tccatgtgag aagtttaatc tagctgcatt cataccaatt cttatgaatt
     9541 ctcttagaat ttcttctgaa tcacttgaag gaccgattgt aaatatcatt tttgtttttt
     9601 gcataaaaac cacctcattt ttttaaacta aaatagttta gtgattgctt taaaagtttg
     9661 atatatttga tataatataa taaattatta aattaattat attttaccac aataatagga
     9721 agggatgggt aaaaaatgaa tatattaaaa ggtttagaac cacaaagtgt tttaaaatat
     9781 tttgaagaaa tatcacaaat ttcaagagga tcaggtaatg aaaagggaat aagtgatttt
     9841 ctagttaact ttggaaaaaa tttaggactt gaaacaatac aagatgaatc attaaatgta
     9901 ataataagaa aacctgcaac tccaggatat gaaaatgcac caggagtaat aatacaaggt
     9961 catatggata tggtatgtga aaaaaataaa gatactatac atgattttga aaaagatcct
    10021 attaaactta gagtagatgg agattatata tacgctacag gaactacatt aggagcagat
    10081 aatggtatag cagtagctta tggaatggct gttttagctt caaatgatat agcacaccct
    10141 gctatagaac ttttagttac aactgatgaa gaagttggaa tgggtggagc tattgcttta
    10201 gatggaactt tattaaaagg taaatatctt ttaaacatag attcagagga agaaggaaaa
    10261 cttttagtaa gctgtgcagg tggagctaga agtgaagtta ctttaccaat aaactttgaa
    10321 gaaatggaaa aagattttga agtttatgaa atcatgctaa gaggactaaa gggtggtcac
    10381 tctggaatgg aaatagataa acaaagagga aactctaata agttaatggg aagagtatta
    10441 aatgatatta atgctaactg tgatattaga ttaatatcaa ttaatggtgg atctaaggta
    10501 aatgctattc caagagaatg tgatacttta ctagctgtta aaaaagaaga tgttaaaaaa
    10561 ttagaagaat taattcaaaa atgggattca atattaaagg atgaatatca tgctaatgat
    10621 agtggagtta atgtaacttt agttaaaaaa gaagaaaatc ataaagtatt ttctaaagac
    10681 actacattta aagctataaa aataatgaac ttaattcctg atggagttga tacttatagt
    10741 atagaaatga aaggattagt tcaaagttca acaaacctag gtgttgttac tacagaagga
    10801 gataaaattg tctttgctag ttcaacaaga agttcagttg aaactttaaa aactaaactt
    10861 ttagatgaaa tagctgatgt tgcagaaata ttaggtggag aatttgaaat acaagcacca
    10921 tacccagctt ggcaatataa tccagattca aaaataagag aactttgcag caatgtatat
    10981 aaaaatatga caggaaaaga tcctgaaata atagctatac atgctggatt agaatgtgga
    11041 ttattaggag aaaaaataga aggattagat atgatttcat ttggtcctaa tatgtatgat
    11101 gttcatactc caaatgaaca tgttagcata tcttcagtaa aaaatgtttg ggatttctta
    11161 gttgaaatat taaaagctat aaaataatta agaattaatt taaggttgta tctaaattac
    11221 ttaaaaaact tataaatata tttttagaat tattaaactt ttataagggg ttattaaagg
    11281 tttctaataa atccttataa agctttgaat ttagcttaag cctatgattt ctatgagaag
    11341 aatttaaatt ctaaatttta tctataatat tacttataag ttttactatt aaggctagat
    11401 acagccttat tattttatag agatataaat aaacatataa aaagaataaa ttaaatacat
    11461 attagactat gggaggatat agacatgaat ttagtaagaa ctgatttaca tatgcatact
    11521 agagcttctg atggaacact tacaccaaag gagttattaa aggaaatagt agaaaaagac
    11581 ataaaactat tttcagttac agatcatgac tctataggaa gcttagagga aatgcaagat
    11641 ttaacggagg gaatggatat taaattcatt ccagggatag agatttcaag tataataaat
    11701 ggagaacagt ttcacatatt agcttataat tttgataaaa ataataaaga gttaatgagt
    11761 ttaatagaaa ataatgatag actgcttcaa gagaaagatg ataattcaat aaagcaatta
    11821 attgatgctg gatatgacat agattttgaa gactatttat cttatgaaca tgatccttca
    11881 aagggtggat ggaaaactct taacttttta attgatagag gaatatgcaa aaatattgat
    11941 gagtttttta ataaaatatt tgttggtgaa agagctttga aatatccaga ttttcctcat
    12001 cctaaagaag ttatagaaac tataaaaaaa gctaatggaa ttgttgtttt agctcaccct
    12061 aagtatggaa agtctcaatt taaattagat gaaatgttag aattatttaa aaattggggt
    12121 gtagatggaa tagaatgtta tcatccacat catgatgaag atacaataaa atacttagta
    12181 gattattgta aaaaaaataa tctaataata acaggtggat ctgattatca cggaggactt
    12241 atatctaaga gaagtttagg aacaccagag ttctatgcaa aatatgattt gttagataaa
    12301 taaattgtta ttaaaataat atattaattt agtttttaag ctttattgaa aataatgctt
    12361 tatggaattt atagatttga attctaaagg caaatttcaa taaagctttt ttatttatta
    12421 ttaaaatgta aaatttaaga aaaataaaat taaaagaagg aattttacaa attatgtaaa
    12481 ataaatatat aaagaggggt tttacataat ttataaaaag aaaaatgatt agtaaagaga
    12541 attaaaagaa aggttggggg aagggtgaag tacactagaa ttgatgtaaa gaataaaaaa
    12601 aggagtaatg gacataacaa caataataat aatgatggta aaagttttat attacttttt
    12661 attggggtaa ttgttgtggc acttgtaata tcaactgcaa tgtctaagtt tatatggcct
    12721 gaaggaaaga aaacagcaga aaaggaaaaa atagaagtaa cttctggaga aaataaagag
    12781 acaaatgaga aagataagga aaataacaaa gtagcaaaag aagatgaaaa gccaaaagac
    12841 aataaggtaa tggctgaaag tgtaggtgga caagagtatg taatgattca atgtggtttc
    12901 tactcaaata aagagaatgc aaataatgta aaggctcaat tagaagatac ttatattgca
    12961 gtatcattaa gtgaagcaga aaattataga gtaatagttc acattggaaa tgaagaagaa
    13021 gctactaaac tttctaatga gttaaatgaa aaaggtgtta gtaatacaaa aggaagattt
    13081 atgattccaa aggctgatgt gtgtagcaat gagattatag agatagtaaa tggttatgta
    13141 aatataataa ataaattaaa agaggattct gttaagggtg ttaagacaac agagtttaaa
    13201 gaatgggcta atagattaga ggagcatagt gattctaaat attataaagt gtttaaagaa
    13261 ttaaaagatg gtataaataa aattccagat gaaataacta aaaaaaatat agaggaaagt
    13321 taccaaatag tttttaatac tttaaataat tttaaagtaa acaaataatt gtaatttatt
    13381 tttaatttat agtaacatat ttttaaatta taataaaaat gaaagagtta aattctcttt
    13441 catttttttc tttataattt actatacacg tacatttata cttgtttata aaaataataa
    13501 atgataaact atataggata aagaatacaa gaccttattt aaggaaaaat aagattattt
    13561 aaaagttttt tagatataag gtaaggtgga aaaatgaagg ttattttagc atcaaaatct
    13621 ccaagaagag ttgagattct tgaaaaaata gttaaagaat ttgaagtagt tcagtcaaac
    13681 tttgatgaaa atactataga ctttaaggga gatgttgaaa agtatgttaa agatttgtct
    13741 agaaataaag ctatagaggt ttcaaaaaga cttaatgagc catctatagt tattgctgcg
    13801 gatacagttg tttttcaaaa tgggaaagtt ttggaaaaac ctaaaagtga agaggatgct
    13861 tttagtatgt tatcctcatt aagtggtaat actcataagg tatattcagg tatttgttta
    13921 ataaatactt atgatgatac tgtagtgact gattgcgact gtacagaagt aaaattttca
    13981 gaattaaatc ctagacaaat aagaaattac ataaattctg gtgagcctat ggataaagca
    14041 ggagcttatg gaattcaagg attaggtggt gcttttgtag agagaataga gggatgttat
    14101 tacaatgtca tgggattgcc cttgaataaa ctatataaag cattagaaaa ctatgacata
    14161 acaatacttt aaaattactt tcaatgggca tgttaatttc taaataagga gttgtaatat
    14221 gtctgaaaat ataaaaatta atgaaattcc atctggtgaa aggccaagag aaaaactact
    14281 tttttatgga gctcagtgtt tatctaatga agagctttta gctataattc ttagaaccgg
    14341 aaataaggat tcaaatgtag ttgaattgtc ttatagaata atacatagtg ttggtggatt
    14401 gaatggactt tttaaagctt cagcaaagga gcttatggag ctaaagggtg ttaaggaagc
    14461 taaggcaact caaattttag ccatgtgtga attatataag agatttaaag tttctgagtt
    14521 aacacagatt aaaatttcaa agccatcaga tgtagcaaaa ttagttttag atgaacttag
    14581 aatgctacgt caggaagtgt tgatcctaat aaaccttgac actaaaaata aagtaatatc
    14641 taaaaaagaa atttttaaag gtgggctaaa ttcttcttta gtacacccaa gagagatatt
    14701 taaagaggct gttaaagata gtgcagcgtc tataattata tgtcacaatc atccttcagg
    14761 agacccaaca cctagtagag atgatataaa cataacaaca cgactaaaag agtgtggaaa
    14821 aatgatgggt attgagcttt tagatcattt aataatagga gataacagat tcataagtct
    14881 taaagaaaaa gacatattat gattaggaaa ggggattaaa aatgggttta tttaattttg
    14941 gaatgacaaa agacatgggt atagatttag gaactgcaaa tactctagtg ttcgttaaag
    15001 gaaaggggat tgtattaaga gaaccgtcag ttgttgctat aaacaactta acaaaaaaat
    15061 cattagctgt tggtactgaa gctaagcaaa tgataggtag aacaccagga aatatagtgg
    15121 ctataagacc attaaaagat ggagttattg ctgattttga cgttactcaa acaatgctta
    15181 agagattcat agaaaagata acaaataaaa gtgcattcac aagcccaaga ataatagtat
    15241 gcttcccttc aggagtaact gaagttgaaa gaagagcaat agaagaagct actaaacaag
    15301 ctggagcaag agaagtaata ttaatggaag agccaatggc tgctgcaatc ggagcaggac
    15361 ttccagttga tgagcctaca ggaagtatga tagttgatat tggtggaggt actactgagg
    15421 ttgctattat atcattaggt ggaatagtta ctagcaaatc attaagagtt gctggagatg
    15481 agttagacca atcaatcata aactacatta agagagaatt taacttaatg ataggtgaga
    15541 gaacggcaga acaaataaaa atggaaattg gatcagctta caaagttgat gagaatgaag
    15601 aagaacaaac aatggacata aaaggaagag atttaatatc aggacttcct aaggttgttg
    15661 tggttgatgc gggacaaata agaaacgctt taaaagagcc agtttcatca ataatagaat
    15721 caatcaaaac tactctagaa aaaacaccac cagaattagc agctgatatc atggataaag
    15781 gaataatgct tgctggtgga ggggcattcc taaaaggact agatgaactt atatataaag
    15841 aaacaggaat gcctgttcat atagcagaat cacctcttga ctgtgttgca ctaggtacag
    15901 gaaaagcttt agataagttt gatttgatag ttaaacaaca aagagggtag aatatgaaat
    15961 tcttcaagaa taaactggca gtaactgtta tattactgtc agttgccttt ttaggaataa
    16021 ttatatttac tgttaagagt gattctaaaa atccaatttc aggtggagtt ggtactgttt
    16081 taaaccctgt gcagaaggtt gtatacacaa taagtgataa agttcatgat gtctttgatt
    16141 tcttctataa cttttcagat gttaagaatg aaaataatga tttaaaaaag aaaaatttag
    16201 aattagaaaa tcaattagtt gaatacaatg agttgaagcg tcaaaatgat gttttaagag
    16261 gaatagttga tttttctaag gaaaataatc aatataaata tatcggtaca aacataatag
    16321 gaaaaagtgg aaatagcttt gtagaaggtt acactattga taaaggatca agtgaaggga
    16381 ttaaaaaaga ttacatagtg atttctccag aaggattagt gggagttgta acagaagttc
    16441 actcaaactg gtcaaaaata cagactttat taaatgaaaa cgtagctgtt gcagcaacta
    16501 ttgaaggaga tagcaattca ggagacggaa tagttaaagg atatagaaat ggtagtgaga
    16561 tgcaagctga gataactaac ttacctatga attctacaat aaaagaaggt gagacagtag
    16621 ttacttcagg acttggagga tattatccta aaggaataag aattggtgaa gtaacatcag
    16681 tacaatcaga tgatgttaaa gttatgaaaa ccgctatatt aaaaacatat gttgatttta
    16741 ataatttaca agaattattt gtagttgtac caccatcaga gagagatggt atagttaagt
    16801 attagaaaag agggagtaaa gtgaaaagat taatactaat attaatatgt cttttactat
    16861 ttatagtaga taatactctg gtaccttttt tctctataaa aggtgtttat cctagtctat
    16921 tattcacctt tgctgtacta tatgctctta tgagtgggta ttgggaagcg gtatttatag
    16981 gagttttatc aggtttcctt caagatgttt attttgtaaa tgtctttggg gtaaatatgt
    17041 tagtaaatat gttagtgtgc ttaatagctg cgtatattgg ggaaagtgta tttaagcata
    17101 aaaaaacaat tccagtacta tcagtaggtt tacttaccat aataaagttt tttatagtgg
    17161 catttatatt aaatttaatt aatataagaa taaacttatt aagcttttgg attatggtac
    17221 tttataacgt agttatagga ttctttatgt ataattgggt ttataaatta tgcaacagag
    17281 atcttatgaa aagagaatgg aaaataagtg aaaaataggt ggttacatga gaagttcaat
    17341 aaatggaaaa acgcctatag atagatataa aattctcata ggaataatga taatagtatt
    17401 tgtatctata gtagcaagat tagtttatct tcaagtattt aattatgaag aatttaaaca
    17461 aagagctgat actagatcta tgagatttct atcagaacag gctccaagag gtaaaattta
    17521 tgattcaaat ggggacattt tagctactaa tagacaaaca tacacattaa catttactga
    17581 aacagatgaa tctaagaaaa acttttatac tactatagat aaagtttatt ctattttaaa
    17641 agaaaataat caaagtatat tagataattt taagattaga attaatgaaa atggagaact
    17701 ttattttgat gtttcaggtg atactgaaaa actaagactt ttaaaagata ttggaatggg
    17761 tgataaggtt aaaaatgttc tttttaagaa taaagaagga gatttaactg ctgaggaaga
    17821 agatcaaata gataaagaac ttctaaagat tactccagat gaggctttta atttcttagt
    17881 aaaggaatat aaattatatg acgttttaaa tcctacagag gaagaggtta agtcatataa
    17941 aaatatggaa tcaaaagaga taacagatat attgttaaag aaatattctc ttgaagatat
    18001 aagaggatat atgcttgtta aaaatgctat gaaaatgcaa agtttctcag gatataaacc
    18061 agttacaata gctaaggata taaacaaaga aactgcattt atattctatc aaagacttaa
    18121 tgatttacca ggaatagatg taagcataga gccaatgaga tactatccat ataatgaatt
    18181 agggtcttca ttcttaggat atgtagcttc tataaatgga tatcaaaaag ctaagtatga
    18241 agcaagagga tatgatgctt ctactgattt aataggaaaa gctggtatag aagcagcctt
    18301 tgaaaatgaa ctaaaaggtg ttaagggagg aactacagtt aaggtaaact cttctggtag
    18361 aaaaaaagag gaactttatg acttagaatc ttacccagga aataatgtac atttaactat
    18421 agataaggat attcaatatt cagcagaaac ttcaatgaaa tatcttttag attatcttca
    18481 aacaaggaat acattagatt caggtgtaaa tactaagaat gcaacaagag gagcggcagt
    18541 agctatagaa gtagatacag gaaaggtttt agctttagta agttatccag gatttaatcc
    18601 aaatgagttt gctaagggag agttagatcc aggtgtggcg aaggaatact tccaaccaga
    18661 ctatgaagcc tttgcacagc agtttatagc tagaaatgct ttaaataaaa cagtagatga
    18721 tcttttccct aaaaattcaa aaactggatt aagggaagat ttatatgatc tttacccaaa
    18781 gagattctat aactacgcta ctttaggtat gattcctcca ggatctacat ttaaacctgt
    18841 taccagtgtg gctgccttag aagagggtgc tataactcca agttcaacag tttatgctaa
    18901 tgggcaaaac tttggtcaac catatccaga gatatttggt aaaaatcctg caaactggcc
    18961 aagagataaa aacttttcag gatatgcaga tttatacact gcattacaac actcatcaaa
    19021 ctcatattat tttgactctg ctgtaaagat gtattataaa aatggtggag atccatatgg
    19081 agatggaaga tataagaaga gtagtacgga agctttagac tctattgcaa aatatgcatg
    19141 gcagtttgga ttaggtgttg atccaaattc agataaaaag gcttcaacag gaattgaaat
    19201 atctgaaaat tttggtcagg tttataattt tgaatctttt aagaatcaaa caatagctct
    19261 tatgagattt actttagttg attctcttga aaaaggaacc tttgttaatt cttcttcacc
    19321 tgattttcca caggtgaata taggagttaa tgatagggat tctaaagagt tagcagcggc
    19381 taaggaatct ataaaaaaag atgtagagaa ttctctaaat aagcttggtg atggaaatac
    19441 tattgatgaa aaagctttaa gtgagtcttt aaaaaaggat tttaaaactt tatataattt
    19501 agattctgcc tatcaagaaa gtattaaaaa ctttaatgga ataaaaggta aggatgaaga
    19561 tgaaaaagtt gataaggcaa taaacttaac agttcaaaga atagttgctt gggttgttgg
    19621 agatattgct taccaaatag tttcaccaag aaaccttact gactctgcta taggacagag
    19681 tataagtaac tttactccag tacagctagc aagttatgtg gcaaccttag caaatggagg
    19741 aacaagatat aaaacgcatt tagttgataa aataactgat gctcaaggaa acttagtaga
    19801 agaatttaaa ccagaagttt taaataaagt taatatgtca aaagctactt tagatgctgt
    19861 taaagaaggt atgagaaggg ttaatgagga agttggagga tctgcaaatt ttataatgtc
    19921 aaagttacct attgtgactg gaggtaaaac tggtactgct actttcagaa atgaccaatc
    19981 agattatgga agaacagact acggggttta tatttccttt gctccactag ataatcctaa
    20041 gattgctgtg gcaacagttc tttatgatgg tggtcatgga tattatggat catttatagc
    20101 aagaactatt tatgaaactt attttagaga agagttaaag aaggattatc cggggtatac
    20161 gccaacaaat cttgatggag ctacttatga ttattcattg aatccacctt tagaagctat
    20221 aaaggattca aatataaata gtaaggtaga aaatgcagaa ttagattctt caggaaaacc
    20281 agttgaagga tcacaagata ataattctga aaataaaaaa gaggatgata aatctcaaaa
    20341 taataatcaa gataacaata atcaaaatga taaaactaaa gtagatgaaa ataaagctca
    20401 ttaatataaa acctacagaa ttaatttctg tgggttttta aattattatt actattttaa
    20461 tgaaataatt aaacaaatta taaataggtt tttaaaatac gattgatttt ttcaaaattt
    20521 tactacttta tttcataaag tccttttata aattaaaaat actttatata atataaagtg
    20581 agaattaatt tattgtataa tttaccttaa gaataaaagg ggttggatac ttggaggata
    20641 gtaatttact atgcgtgatg atagaatttt tataaaagga aataaactag gaataaatgc
    20701 cataataaat atggacaagt ttggtaattt tgatgaaatg cttgattctt tagtagagaa
    20761 gttaagtaga ggaaaaaagt tttataaagg ggctacttta actgtaacta ctgatttaaa
    20821 atatataaat gaaagacaaa tatcaaagtt aaaagatgta ctttttgatg agatacttat
    20881 aaaggattgt atatttgaag aacgattaga aaagcaaagt agtgtttttt cgggagttta
    20941 tgagggaaga acaaaatttg taagaaaaac tgttagaagt ggtcaatgtt taaattacgc
    21001 aggtaatttg ataataattg gtgatgttaa taatggggga gaagttagag cacatggaaa
    21061 tgtaattgtg ctaggtgact taaaaggaaa ggtttttgct ggagataatg gaaatgaaaa
    21121 tgccataata gcagcttatt ctttagaacc agaattaatt tctatatcag gtaaaataac
    21181 aatatcaccg gatgattttg agaaaacagg atatccggag gtggctagat tgaatgaaaa
    21241 taatataatt gtagagcctt atttaccgga taaatattct tattaatatt acataattta
    21301 atggagggaa agtaatggga gtatcgatag taataacatc aggaaaaggt ggagttggaa
    21361 aaacaactac tacagctaac attggaacag ctttagcggc acaagggaaa aaagtagtcg
    21421 ttgttgatgg agatacaggt ctaagaaact tagatgtgtt aatgggatta gaaaatagaa
    21481 ttgtttatac agtaattgat gtaattgaaa atagatgtag aactaagcaa gctttaataa
    21541 gagataagag atttaataat ttatacttat tacctactgc tcaaacaaag gataaaaatg
    21601 atataagtcc tgagcaaatg ttaagattag taaatgaatt aaaagaagag tttgattatg
    21661 taattttaga ttgcccagct ggtatagagc aaggatttga aaatgccata gttggggcag
    21721 atagagctat cgtagtagtt aatccagaaa taacttctgt aagagatgct gatagagtta
    21781 ttggaaaact tgatgctaag ggattagaaa atcatgaggt tatagtaaat agattaaatt
    21841 atgaaatgac taaaaaaggt gatatgctag acatttcaga tattatagaa actctttctg
    21901 taaaactttt aggagttgtt ccagatgata gaaatataac agtttctact aataaaggag
    21961 agcctatagt actagatgaa aaagcaagtg ctggacaagc atttagaaat ataggtagaa
    22021 gaataatagg agaagatgtt ccaataatgg atttaaacac agaacatcaa ggaatattta
    22081 gttctatatt aaagttgttt agaagagggt aggtgagaga aaatgagttt tttaaatgtt
    22141 ttttctagta gacctacgcc gaaacaagtt gctaaggata gattaaaggt tattttaatt
    22201 catgatagag gggaactatc agatgaagtt ttggataaaa taagacttga aatattagat
    22261 gtactttcta aatatgtaga gattgaaaat gaagatgttg acataactgt tacaaagtca
    22321 aatgcaatag aaggggaatc accatcttta gtagcaaata taccaataaa aaatataaag
    22381 ggtaaagcta gataatttaa aaatataagc tatttcaaat ttcttgagat agctttttat
    22441 tagtctttta aaaagttcag aattttgtaa ttgaaaatag gtaaaaataa agtattatat
    22501 aaatagttat gttaaataaa taaaatttat ggaggtacta taatgcttag tagactaaaa
    22561 ctagatagta aactaagaag agaaattgat tataagcttt tagtatctat gatattaata
    22621 gtactgtttg gaatattaaa tatatactta gggacaaaat ctcaaagagg attttttttc
    22681 gctaagaaac agttaatatg gtttataata tcaatggctg cattgtacat aatattactt
    22741 tggaattata acataattta taattatgtt gagatattct attggggatc catagttcta
    22801 cttataataa ccagatttgc aggaagtgta ataaatggtg ctagaggatg gattgtatta
    22861 ggtcctgtaa gtattcagcc ctcggaactg gctaaaacgg ctatgatact tatgttggcc
    22921 aagaagatgg aacaagttga tttaagaata aatgacttta gaaattttat taaggttgcc
    22981 atgtatgcaa tgataccaat gatatttata gttgtacaac ctgatatggg tatgactatg
    23041 gtttcatttt tcatagcact tggtatattc tttgcagcag gattggacat taaagtaata
    23101 ggtgcagggc ttttatcgct aatagtggca atagccttag tttggaattc tggaattatt
    23161 aaagactatc aaaaggatag acttgttgga ttcttaaacc ctgatggaga tgagctaggt
    23221 ataaatcttc aattaactca atcaaaaata gggattggat ctggaggatt ctttggaact
    23281 gggttagatt taaatggaga agttggtggt tattcctcag aattcgtacc agaaaggcaa
    23341 acagacttta tattcgctgt aataggtgaa cattggggaa cagtaggagg aatagtacta
    23401 ttattattat acgcaataat gatatataga ataataatga cggctaaaac ctctaaggat
    23461 atatttggct ccataatatg tgttggattt gcctcttatt ttatatttgc tatactacaa
    23521 aatataggaa tgacaatagg gataatgcct ataacaggaa taacattacc attagtaagt
    23581 tatggaggaa gttcgcttct tacaactata gtaagtatag ccttggtttt aaacatatct
    23641 atgagaaaga aaaagttaaa attctaataa aaaataaaag tattaaaatc attaatgatt
    23701 ttaatacttt tattttttta tatctattat atttctcttt gtaataaagg ttctataaga
    23761 tgcataaaaa aaatatatat taattataat taaatatttc tagaaaggaa aagaaaaagt
    23821 gggtagctat aacaaagaat atgagaagta ttatagaaaa ataaataaaa cgagaaaaac
    23881 ttctcctaat catgaggcga atttaaaagc taaaggctct tataagagaa aagagaaaaa
    23941 tagttttgga aaatacttaa ttaaggaagt tgttctctca tcgattttag gcatttttat
    24001 tttttctagc ttttttttta tgggacaagt agaaaatgaa tttttagtag gattaagtag
    24061 gaaatttaaa gaggtaatat caactgatag atattataaa aacttagact taactgaaag
    24121 taaaatagta actactatta gtagtggaat aaaggatggt tttaatttag agaattctaa
    24181 tttaaaaaaa gaacctaaac aagttagtga agaaaaggta tctgaaaata gtggggaagt
    24241 aagtgctttt aacaattttc agattattga gaaggaatct atagactttt taaaggaaag
    24301 taaggtaatt ccttttaatg gagaagccaa aactttggaa aataataatt taaaggaaaa
    24361 aaatctttat ttaagtggtt caaagggaga ggtgaaaagt cttttaaaag gaaccataag
    24421 tgaagtgaaa aatgaaaatg gaacatatac tgtgaagatg aattattatg atgacttaga
    24481 agttttatat cacaatttag aagaagtgct aaaaaaagaa ggggacagta tagaagaggg
    24541 agagaaaata ggtacttcat ctgaaaatga aaaagtaaaa ggtgtaataa ttcaggtatt
    24601 atttaaaaat gagtatgtta atccagaaga aagtatgagg tttttaggag ataagcagtg
    24661 aaaatcgata aaaagataat tatacagctt gttacatttt ttattttagg atttaaattt
    24721 aacttagctt tagctttttt ttggattata atacatgaat tggctcatta ttttgttttg
    24781 ttaaaattaa atatagagtc agagaatttt aaacttcata ttttaggagc aagattagag
    24841 acaaaggatt atgaatgttt aagttcaaga gaaaaattaa tggtatgctt tgcaggtcca
    24901 atttcaaatg gaattgtttt tctaattttt ttctttattt ataaattaat ttttaaaaat
    24961 gaatatattt attccaccta tgaaataaat ttagtcctat tcattttcaa tttacttcct
    25021 acttatccac tagatggagg gaaaatatta ggagcaatat tagagaagaa gatgatcttt
    25081 aaggatgtca ataatatatt gatcaagata agctattcat ttggaatagc atttatttta
    25141 ttgtctatat taggagttct gattttaggt gaaataaata taactagtat attagcagga
    25201 atatttataa tctatttaag ttataatgag agaagaaagg taatgtatat aattatgggt
    25261 gatataacaa aaaagagaga acgattaatt aataagaaat acatagacag tagagtaact
    25321 tctgtatatt gtgaacaaga tatgattaat cttcttagaa ttattgataa aaatagattt
    25381 aatatatttt atgttttaga tgaagaaatg aatttgcttt atgtcttaag agaaaatgaa
    25441 ataatagaga ctttaaagac aattgggaac attaatttaa gagaatatta cattaagtat
    25501 agaaaagaat aaatatattg gttataatta ttctttgtga tagaatagaa attgttaata
    25561 tgagagaagt ataaagaggt atacttctct ttttattata gatattagct ttaatatttt
    25621 ataaaaatgt aggaggaaac taaatggtaa gaataactga tgatatttta tatagagttg
    25681 agaagccatc tagatatgta ggtggagaac ttaatgaagt gataaaagac ccaaaagaag
    25741 ttgatataag atttggattt tgcttcccag atgtttatga agtagggatg tctcacttag
    25801 gatcaagaat tttatatcat gttttaaatc aaagaaaaga tacatattgt gaaagagtat
    25861 atgctccatg gccagacatg gaaaagctta tgagagaaaa caatattcct ttatatgctt
    25921 tagaaactaa agatcctata aatgaattca actttttagc atttactctt caatacgaaa
    25981 tgagttatac aaacatatta aatatgttaa acatgggtgg aattccactt agagcatcag
    26041 aaagaacaga gaaggatcct ttagtaatag ctggtggacc ttgtgcatat aatccagagc
    26101 ctttatatga tatagttgac atattccaat taggtgaagg tgaagaagga ttaaatgatc
    26161 ttttagatct ttatcaaaaa cataaagata actttaataa ggaagcttat ttaagagaag
    26221 ctagtcatat tccaagtgtt tatattccat cattatatga tgttacatac aacgaagacg
    26281 gaacaataaa agaatttata cctaagtacg atgatgtacc taaaaaggtt aaaaagagaa
    26341 tcataaataa ctttgaccaa gttgatttcc cagaaagttt aatagtacct tatagtgaaa
    26401 tagttcatga cagagttgtt ttagaattat ttagaggatg tactaatgga tgtagattct
    26461 gtcaagctgg aatgatatat agaccagtta gagaaaagag cagaaaaact ttattagatc
    26521 aagctaggaa aatgcttaaa gctacaggat atgatgaggt ttcattagtt tcactaagta
    26581 cttgtgatta ttctgatatt caaggattag taaaagatct tatagaagag cacggaaaga
    26641 ataatgtaag tgttgcctta ccatcaataa gagtagatgc tttctcagtt gatctattaa
    26701 aagaaattca aaaggttaga aaaactgggt taacttttgc accagaggct ggttctcaaa
    26761 gaatgagaga tgttataaat aaaggattat cagaggaaag aatattagaa gcagctaaaa
    26821 atgcttttga atcaggatgg agtacaataa aattatactt tatacttgga gttccttatg
    26881 aaacagttga ggatgctgca gaaataggat tattagcaga gaaaatagct gatcaatact
    26941 ttgctgtacc taaacatata agaaataaag gacttagaat tactgtaagt acttctattc
    27001 ttgtaccaaa accatttaca ccattccaat gggcaccaat ggaaaagatg gatgttgtaa
    27061 ctgagaaaat caatgctgtt aaaggtgcta taaaatcaag aagcatagtt tacaactatc
    27121 atgagcaaaa aacatcatat atggaagctg ttttagctag aggagacaga agactttgtg
    27181 atgtattaat aaaagcttat gaaaaaggtg ctaaattcga cggatggtca gaatactttg
    27241 actttgaatt atggcaagaa gctttagctg agtgcaatgt agatggagat ttctatgttt
    27301 acagacaaag aagctatgat gaaattttac catgggattt catagatatt ggagtaacaa
    27361 gaaaatatct tgaaagagaa aatgaaaaag ctaaaactgg tgaacctact caaaattgta
    27421 gaaaaggatg tacaggttgt ggcgtaaatg taaactttaa agatggggag tgttttgaag
    27481 gtgcgatact taattaaatt tactaaagat gcagatataa aatttgtttc tcacttagat
    27541 ttaatgagaa caattcaaag aatagtaaga agagctgagc ttccagttga atattcaaaa
    27601 ggatttaacc cgcatatggc cttagcaata gcacaacctt tatcagttgg tgtatattct
    27661 gagggagatt atttagactt aaatttaact gaagatatga atgaagagac agtaaaagaa
    27721 aagttaaatg aattctcagc aaatggtatt agatttttag aagtttcaaa gagtccagtt
    27781 atacacaatg ttaagagatt gccagcagct atggctcttt tagatgctgc aaattataca
    27841 ataaaaataa gatataatga tatttctaag gctgaagaag aagttaatgc tttaatggct
    27901 aaaaaagaaa taatcacaat aaaaagaact aaaaaaggtg aaaaagaagc ggatataaaa
    27961 ccatttataa aagattttaa atgttggact aaggacaatt atttgattgt aaatacaact
    28021 ataagctgtg gaagtagaga aaatctttca gcagatcttt tagcaaatgt tataaaagaa
    28081 aatacatcaa atgttaatga agaagctttc gtagaaataa aaagagttga gatgtatgca
    28141 tacaaagggg atacattagt tcctctatat aaatacatct aaattaggaa gtgaattcat
    28201 gagagatata tacgtagaaa gaagagaaaa tttattaagg gttgccataa aggaaaataa
    28261 tcttttaagt gagtgctata tagaagaaga gagtctagaa cctgttcaag gagagatata
    28321 taaaggtata gtgaaaagag tagttccagg aataaagagt gcctttatag atattgggta
    28381 tagtaaacag gcctatatga tacttggaga agatgagaaa gatcttaaac aaggagatgg
    28441 aataattgtt gaagtattaa aagagcaatt tggaagtaaa ggggcaaaag ttacttcaaa
    28501 aatatcttta gcaggaagat attgcgtctt agaaacttca agaaatgata tttcaatttc
    28561 aagaaagata gaaaatctag agtttaaaaa agaacttgaa aattcattga taaaaccaag
    28621 tgaattaggg gttactgtta ggacaaaagc ccaatcagct agtatttttg agattcaaga
    28681 agaaatagaa gaactatata aaaactataa agaggttatt agagaaggta aattttcctt
    28741 aaatccaaaa aaaataatgg gtaaaggaga ccttattaat aagataatta gagatgacat
    28801 tgatgcttat acttcttcaa taattgttga ttctatagaa gattatgaaa gaataaaaga
    28861 tagtatttct aattctgaat atttaaaagt aactctttat gatgatgcta gaagcctttt
    28921 tgattattat ggtatcgaaa aagaaattct tagccttaga aataatagag tgaatttaaa
    28981 ttgtggcgga gacatagtta tagaaaaaac agaggctatg tatgttatag atgttaattc
    29041 aggtaagaat acaaatcata aaaataaaga aaaaactgcc tatgaaacta ataaaaatgc
    29101 ggcaatagaa attgctagac aaattaaaat tagaaattta ggtggaataa tattagttga
    29161 ttttatagat atgtattcac aggaaaacaa gaaaaaaata ttaaaaatat taaaagatga
    29221 atttaaaagt gatagccaaa gagctaaaat ttttcctttt acagagcttg gattagtaca
    29281 aataacaaga tatagaaaag gaaaaagcat ttatgaatat atagaagaaa attgcttagc
    29341 atgtaatggt catggaaaga gattaaagtt atcttatata aatcttcttt taagaaatga
    29401 gattttgaag aaaaatggag aaggaaagat aaaagatttc catatagaaa taaactctat
    29461 ttatgaaaaa gatattagag gaaatatttt tgagttttta aaagaaattg gagcattaga
    29521 tttaaatatt tacttgaatt ttcaagaatc taaagacttt tataaagtag aaccattgat
    29581 ctttaaaaat caaatagaaa gcgtaaaaga gtatttggtg aagaatatcg aaaaatatta
    29641 aatttagctt tacaaaaggc tttagctatg atagaatggc ttttgtaaac cgctcacgaa
    29701 taggttttaa aaataacttt aattagttta ccttaagtgg cgagaccgtt atgaggaggt
    29761 gttttaatgt acgcagtatt aactacagga ggaaaacaat acagagttca agaaggagac
    29821 gtattattcg ttgaaaaact taacgctgaa gttgactcaa cagttgaatt aacagaagtt
    29881 cttgcagtcg ctaaagacgg agaaatcaaa gttggtgcac cagtagttga gggtgctaag
    29941 gttgtagcaa aagtattagc tcaaggaaaa gctaagaaag ttgttgtatt caaatacaaa
    30001 agaaagaaag actacagaag aaaaaatggt cacagacaac cttacactaa gatcgttatc
    30061 gaaaagatcg aagcttaatt ttctgatatg attagagtta aatttcaatc aaaagaagat
    30121 aacataattt ccttcactat taaaggacac gcaatggaat gcagagatgc tattctagct
    30181 ggagaggcgt acgatatgat atgtaattcc gtatcagtat tatctcaaag tgcaatcatt
    30241 ggtttagatg aggttttaaa acttaatgta aactatgaaa tgagtgatgg atatttaaaa
    30301 ctagatctaa atgggtttac tcatgaagaa atagttgaag ctcaagtttt acttaaaact
    30361 tttgaaatga gcttagcgag tctagtccta gggctggata gcagtttagg aaagaaaacg
    30421 cgttgtaaat atatagagat aataaaagag gaggtgtaga ctatgttaaa aatgaacctt
    30481 caattatttg cacataaaaa aggggttggt agctcaaaga acggtagaga ttctgaagct
    30541 aaaagacttg gtgttaaatg tgcagatgga caattcgttc ttgctggaaa catcttagta
    30601 agacaaagag gaacaaagat tcatccaggt gctaacgtag gtaaaggtgg agacgatact
    30661 ttattcgcta agatcgatgg tgttgttaaa tacgaaagac ttggaagaga caagaaaaaa
    30721 gcttctgttt acccagtaaa cgtagaagaa gttatagctg aataatatat ttagttttat
    30781 aaaagcaccc ttacttaggg tgctttttga ttaataataa aaaaatttaa ccatatgaga
    30841 acatagcatt aaaaaagctg tgtttttata ttaaagtaag gttataatat actaaaaagg
    30901 aacattttat agagaaagca aggtgagaaa acatgtttat agatacggcc aagatatttg
    30961 tgaaatctgg agatggtggt catggttctg tttcttttag aagagaaaaa tacgttccac
    31021 ttggtggacc agatggtgga gatggtggaa aaggtggaga tgttactttt gtagtagatc
    31081 caggaatgac aactctttta gatttcaaat ataagagaaa attcgtagca ggcagaggac
    31141 aagacggtca aggttctaaa tgttacggaa gagatggtga aaatttaaca ataaaggttc
    31201 caatgggaac tataataaga gatgttgaaa ctaataaggt aatggcagac ctttctcata
    31261 gagatgatac atatacaatc tgtagaggag gaaaaggtgg taagggaaat tgtaagttct
    31321 gtacacctac aagacaggcg ccaacttttg ctgagccagg aatgccaggg gaagaaagat
    31381 gggttgcttt agaattaaaa ctattagcag acgtaggtct tttaggattc cctaacgttg
    31441 gtaaatcaac attactttca gtggtaacta aggctaaacc aaagatagct aactatcact
    31501 tcacaacttt aaaacctaat ttaggagttg ttgctgtacc aggaatagag ccatttgtta
    31561 tggcagacgt accaggtatt atagaaggag cttcagaggg tgtaggtctt ggattagact
    31621 tcttaagaca tatagaaaga acaagacttt taatacacgt tgttgatatt tcaggagttg
    31681 aaggaagaga cgcagttgaa gacttcaaga gaataaacga agaattaaag aactacagtg
    31741 ttaaactttg ggatagacct caaatagtag tagcaaataa atgcgatatg ttatttgatg
    31801 aagaaatttt tgaaaacttc aaagctgaag ttaataaaat gggatttgat aaggtattta
    31861 aaatgtcagc agctacttca caaggagtag aggaagtaat aaaagaagca gctagaatgc
    31921 ttaaagatat tcctgttact gacttagaaa tcccagaaga tgaaagattc attccagagg
    31981 ataagaagtt tacatatact ataaatccaa tagaagaaga tggattaaag gtttatgtag
    32041 tagaaggttc atttgtagac agattattat tagctgttaa tgtaaatgat cctgattcat
    32101 taagatattt ccataaggtt cttaataata aaggaatatt ccatgagtta agagagatgg
    32161 gaatagaaga tggagatatg gtaagattaa acgactttga gtttgaatac ttactataat
    32221 taatggagga taaataatga taacaacaaa acaaagagca tatttaagag gattaggtgc
    32281 taaattaact cctatatttc aaataggtaa aaacggaata gaagataact ttttaaagca
    32341 agttgatgat gctttagaag caagagagat tttaaaaata aatgttcttg aaaatagtgg
    32401 attagagaca agagaagctt caaatttcat ctgtgaaaga ttaggatgtg aaggggttca
    32461 atctatagga aacaaaatag ttctttacag agcatcaaaa aataatccta agatagaatt
    32521 acctaaaaaa gggaagtaat atgaaaaaaa taggagtatt tggtggaact tttgacccaa
    32581 tacacatagg ccatatttat atagcttatg aagcctataa aatattagaa ctagatgaag
    32641 ttatattcat gccagcaggc aatccacctc ataagaagtg gaaagatata actgatgaaa
    32701 taattagata tgaaatggtt aaaaaagcta tagagccata tagctttttt tccataaata
    32761 actatgaaat agagaaaaaa ggattgagct ttacttatga aactttaaga tatttacatg
    32821 aaagttttaa agaagtagag ctctatttca taactggagc agattgtctt gtaaacctaa
    32881 attcatggaa aaatataaat gaaattttta aatttagtaa tttagtagtt tttaatagac
    32941 ctggatttga taaaaatgat ttattaaaga ggaaagaaga gtttgataga gaatattgta
    33001 ctaatatagt atatttagat ttacttaata tagagataag ttctacactt ataagagaaa
    33061 gagttaggca gtctctagag gttaaattct ttttaccacc aggtgtagtg gatataatag
    33121 ataaatataa tttgtatagg agagagtaac tatgtggtca ttagagaaaa taaataatta
    33181 tgttaaagag actattaagg gaagtagata ctcacatact ttaggggttg ttgaaactgc
    33241 taaagaactt gctaaaataa atggcgaaga tatacaaaag gcagaattag ctgcattaat
    33301 tcatgatgta gcaaaatata ttcctgtaga tgaacaagta aaaatattag aagagcatgg
    33361 ttatgaacta gatgaaataa ccttaaaatc acctcaagtt ttacatggat ttgttggtgc
    33421 catagtagct aaagaaaaat ttggaataga tgatgaaatg gttttagatg ctgtgaaata
    33481 tcatacttta gccaaaaaag atatgagtac tctagaaaaa ataatatata tagctgacta
    33541 tattgagcca ggtagagatt tcccaggggt tgaagaatta agagaaataa caagagaaga
    33601 tttagataaa ggagtattaa aaggcttaga aaatacaatt ttatttgtaa taaaacaagg
    33661 caatttaata catcacttaa ctatagaagc aagaaatttc ttaataatgc aagaaaaata
    33721 atcaaataat tggagagggt tatatgggga atgaagaaaa tagatttgaa aaaagacaag
    33781 ataagaaaaa tataaataaa aaaagcttga taaaaagaat aattttaggt gctttagctt
    33841 ttattcttat tttttttata agtatttttg gatattttta ttttaaatat ggcagttttt
    33901 taggagaagt ttttaatata gttaaagacg taaaacagga tgtaataaag gttgaagagc
    33961 caattaatgt actcttatta ggaatggata ttggtgatac aagtgatgta tcaaataaag
    34021 atgctaaaag aacagacact ataattctag caaactttga tcctataaca aaagaggcta
    34081 agttaatatc tatacctaga gatactagag taaaaattgg aggaaagtct caaaaaataa
    34141 atgctgccta cccaattggg ggagaaaagc ttgttaaaca gcttgttgga aatatattag
    34201 gggttaaggt tgattatgtt gttaaagttg attatgaagg ctttagagga ataatagatg
    34261 ccattggtgg aattgacatg tatatagagc atgatatgaa ctatgatgat ccagggcagg
    34321 atttacacat acatttcaac aagggggaaa ctgtacactt agatggtaaa aaagctgaag
    34381 agttttttag gtggagaaaa aataatgatg gtacaggatt agtaaatggg gatgttgata
    34441 gaataaaaaa tcaacaaaag tttataaatg ctgtaataga taaggtttta tcagtatcta
    34501 ctataacaaa gatagatagt atagcagaga ttttaaataa aaatatagaa actaatattc
    34561 ctttagatgt tactgttgca tatggactta aaggattaga agctgataag aataatataa
    34621 aaatgatgac tttacctgga gaaggtaaat atgttggaaa tgtttcttat tatattctag
    34681 atgaaaaggc tactaaagaa ttaaaaagtc aattaaacag tagtaattta agtggaagaa
    34741 aaaatgttat tcctagtgct gctagaggag atttatccat aaaagtttta aattgcacaa
    34801 aaataaatgg cttagctgca aatgtacaaa aacaattaaa tgaaaacgga tatgataaag
    34861 ttgatgttgg aaatggagat ccaatagaaa aatcagagat tttgataaag gatgaaagtt
    34921 ctagagcttt tatggaaaat gatttaaaga tagataatat taaaaagggt atttcatcta
    34981 agtatgatgg aaatgaaact tatgatgtag taatattatt aggaaaagac tttaaaaatt
    35041 tcggagagat gaaatgagta ttttagaaag taaaataact aaagagtatg ataaaagaaa
    35101 aataagagaa tttttaaaag aagagttagg attatcttca agattaataa gaagtgcagc
    35161 tatagacaag agaattaagg tagatggtgt tgctgtaaag atgaactatg ttgttaaaac
    35221 tggtgaagag ataaaaatcg agctttcaaa aaaggaatct caaaatattg aaccagaaaa
    35281 aattgatata gaaatagttt atgaggatag tgacatatta gttttaaata agaagccttt
    35341 tatggttgta catcctacaa gaagccatcc taatggaact ttagctaatg gtatattata
    35401 ttactttaaa gagagtaatc aagattgtat agttagacta gttagtagat tagatatgaa
    35461 tacttcagga cttataatca taggtaaaaa tcaatatgct catatggttt tatctaatga
    35521 aatgaaaact gataaatttc aaaaaagata tttagctata gttcatggaa atttagaaga
    35581 aaaagaagga actatcaata gaccaattta tagaccagca gaggatacta taaaaagggt
    35641 tgtagatgaa agagggcaag agagtataac tcattataag gttatagaaa gttataatgg
    35701 tgctgattta gttgaatgtt tattagaaac tgggagaaca catcagataa gagtgcatct
    35761 aagtagttta ggtcatccta tatttggaga ctctttatat ggaacagagg atgaagagtt
    35821 tatatcaaga caggcacttc atgcctatgg attagatttt tatagtccaa agacaaagaa
    35881 gcctttaagc ttaagggcag atcttccaga ggatatgaaa gagttaataa ataaattaaa
    35941 aaacaagtag ttttaagcca atgcttaaaa ctacttgttt tttaattata aaaatatgaa
    36001 ttacgttttt ttctatttct tattcttttt atagttgata taagtctaat cataaatagt
    36061 attaagatta ttaaaaatat taaacctata acacctaaag ctatgtaaaa tccactaaaa
    36121 ccctttagtt taaacataat tttttcaaaa cttgtataag ttatatcatt actatttaaa
    36181 agatttaagg ttttataagg agtatttcca atcataagta ttccagtaaa agaatcagta
    36241 tcctttaata tgtcagcacc cctagatata ggtttagttg cttttactga aaagtctatg
    36301 ttaaaatttt tctttatttc atctgtagat aagttctcta tttcagagtt cttaaatgtt
    36361 ttgtaaatat cttcaggagc taataaagat aagttatttt tatcatcaat atagtattct
    36421 tttataacat catttttaga atataattta actaaagaaa aattattaaa tccataatct
    36481 aaaagatttt ttgtatcttc atagtattga tccttagtgt cataacctag tagggtaaga
    36541 attaattttc tatcacctct tttagctgca caagtaaatg tatgccttga tttagttgta
    36601 tatcctgttt tattacaaaa aatatcttta ttataatatg ggcttgtttg atgaataagt
    36661 ttattattat tatttgccca tataggttct ccacttacaa tgcttttagg catttcataa
    36721 actgtttttt ttgtaatttc atcaattaat ggattctttg aagcggcatt cattattaaa
    36781 gctagatcat aaggagtagt catatgatca tcttcaaaaa gtccactagc attaacaaag
    36841 tgagtatttt tagctcctaa ttcttctgca cgctcattca taagttttgc aaattctcct
    36901 tcggagccag aaatatgttc agctaaagct acagcagcat cattagctga ttgtattaat
    36961 aatgcattaa gtaattcttc aactgtatat ttatctcctt cttgaagacc aacacgggtt
    37021 ccttcagcta aaggaggatt ctttcctata gtaacaacat cgtttagttt agctttttct
    37081 actgtaagta aagctgtcat agttttagta gtagatgcag gagcaagttt ttcattagca
    37141 tttttttcag ctaaaacctc acctgtagtc ccatccataa gaaccattcc ttcaccttta
    37201 atttcaggta cattactttc agcaaaaaca ttagtactta aaattaaaga gaagataaaa
    37261 gccatagata ttataaaatt aagtcttatt tttaaattct taaactttat actcatagag
    37321 cctccaaaat ttttagtttt aattgcaata attattataa caacaataga gtgtattttc
    37381 tataaatttg gtactcaaaa atagataatt tgtcaataat attaaagaaa tatgagagtt
    37441 tattgattta aaactttatt tttgataaat ttctaagaat ttatataata tggaggagtc
    37501 atgaagagaa aagaaaaaat tataggtagt ttaataattt ctgtaatagc tattttattt
    37561 ttatttattg gttattcaaa gacaaatgat aaaaaaataa gtgatagtga tatggaaaaa
    37621 ctatttatag ataaagagga gtcagaagag aataatgaat tagaagattt caatgaaaaa
    37681 gaatcaagtt caaaaaaaga agagagtaat aatattaaag aagaaaaata tagtgatata
    37741 gaaagtgaaa aagaagggaa aaaaactagc ttagatgaag aaaaaataat tgtagagatt
    37801 aagggcgagg taaaaaagcc taatgtctat gaattaaaaa atggaagtag aatttatgaa
    37861 cttatagagg aagcaggagg acccactgat gaagcggatt taagtaatat aaatagagct
    37921 ttatatttaa gtgacgggca atgtatagtg attaaaaata taaatgatgt tgaaagtgaa
    37981 gaggcaaatt taaatgaatt tttaaatgct gaagttacta atagtattcc tactaattca
    38041 tcaggtgaca aaggtgagaa aaatgaaagt tcaattataa atataaatac tgcctctaaa
    38101 gaaacactta tgaccttaaa tggaatagga gaaagcaagg cacaggccat aatagattat
    38161 agagatgaaa ttggaggatt taaaagtgtt gatgatatta caaatgtaag tggtatagga
    38221 gaaaaaacct tagagaagat aaaagataaa atttctataa agtaatatga tataatgaat
    38281 attattattt agaaaagagg tggagtttta tgatagaaaa aagattaact gagctatcaa
    38341 agacttctgg ctgagcagcc aaaatagggc cgggggctct ttcagaaatt ttaagtaaat
    38401 taccaaagat gaatgataag aatttaattg ttggaataga tacatctgat gatgcagcag
    38461 tatataaatt aaatgatgaa atggcaacaa tacaaacatt agatttcttt actccaatag
    38521 tagatgaccc atataccttt ggacagatag ctgcagcaaa ctccttaagt gatgtttatg
    38581 ccatgggagg aaagcctata gtggcattaa atatagcttg ttttcctaat tgcttaaata
    38641 tgaatatatt aggtgaaata ttaagagggg gagcagataa ggtattagag gctggagctg
    38701 taatagttgg aggacataca gttcaagatg atgaacctaa atatggttta tctgttacag
    38761 gaattgttca cccagataaa gttcttaaaa actatggagc agagacaggt gatattttaa
    38821 tattaactaa accaattggt cttgggataa taaatacagc tattaaagct aagatagctt
    38881 ctaaggaagc ttatgaaaaa gcagtaaaag taatggcata tttaaataag tatgcaggtg
    38941 aaataataac tgactataat ataacatcat gtacggatat aacaggattt tcactaattg
    39001 ggcatgcgta tgaaatggca gaaccatcta aaaaaacctt tagaatcttt aaagatgcaa
    39061 ttccatttat taaagaagct aaagaatatg ccagtatggg attaatacca gctgggtgtt
    39121 atgagaataa aagatactta gaaggaaaat atcttctaaa gaatgttgaa ggttggatgg
    39181 aagatatatt atttgatcct caaacatcag gtggattatt aatcagttgt aaagagaaag
    39241 attacataga catactaact agattagaaa atttagaagt agaaagtgct gttatagggc
    39301 gtgttgaaga ctttaatgat gcatatatag tagtagagta atttttagga ggataacatg
    39361 acaaaagaac ttcttagagc tttgcctaag atagatgaaa tacttggtat ttttaatgag
    39421 gactttttaa atgaaaatgg gagagaaaca gttgtaagtg ctttaagaga tataatcaat
    39481 gaaaatagga aagctattct aaatgaagag gtagattatg ccttaactaa ggaagaagct
    39541 aagagtaaat gtgagcatag attacttaaa aaaagagaaa gaaatttaaa aagggttatt
    39601 aatggaacag gtgttgttat tcatactaat ttaggaagat ctttgttaag caaagaggcc
    39661 actgaagcag ttgctttagc cgcttcaagc tatagcaatc tagagtatga tttagaaaag
    39721 ggagaaagag gatctagata ctcccttata gaaggtatca taaaagatat tacaggagct
    39781 gaggcagcct tagtagtaaa taataatgca gcggccataa tgcttgtatt aaattctctt
    39841 tgtgaaaata aagaggtaat tgtttctaga ggggaacttg tagaaatagg gggctctttt
    39901 agaattccag aagttatgaa tttttcaaga gctaagcttg tagaagtggg aacaactaat
    39961 agaacacatt tatatgatta tgaggatgcc ataacagaag aaactggagc ctttttaaaa
    40021 gttcatagct caaactttaa aatagtaggt tttactaaga gtgtaagtgc taatgagata
    40081 tgtaacttag caagggaaaa ggatatacct gtaatagagg acattggaag tggtgtttta
    40141 attgatttaa gcaaatatgg attagaaaaa gagcctacag taatagaaag tttagaatct
    40201 ggagtagatg ttgtaacatt ttctggagat aagatgcttg gtggagcgca ggctgggatt
    40261 attgtaggta aaaagaaatt catagataaa ataaaaagga atcaattaac tagagctcta
    40321 agagttgata aatttacttt agctgcttta gaaattactt taaaacatta tttaaatgaa
    40381 aaagaggcaa ttgaaaaaat accgactctt catatgatga ccttagattt aaaggaaata
    40441 gaagaaagag ctaatagact ttataagaat ttagaagggt taaataagtt ctataaattt
    40501 tctatagaag aaggtgaatc aacagttggt ggaggttcaa tgccagatag taaattaagt
    40561 acttatttac ttagaataga ttcagatagc ataaatgaag ttaatttaga aagagagcta
    40621 agagaatata aaatacctat aataactaga gtttataaag gggcagttta tatagatctt
    40681 agaacaatct tagaagatga ttatgaagtc atatttaatg ctttaaagga aataggtgaa
    40741 aaatagatga aacatgtgat tataggaaca tcagggcata tagatcatgg taaaacaact
    40801 cttataaagg ctttaacagg tagggaaact gataagcttg atgaagaaaa gaaaagaggt
    40861 atatcaatta acttagggtt tactttcttt gatttgcctt caggaaaaag agcaggcata
    40921 atagatgttc cagggcatga aaaattcatc aaaaatatgt tagcaggagc tacatcacta
    40981 gatgtagttc ttctaattat tgccctagat gaaggaataa tgcctcagac aaaggaacat
    41041 ttagaaatat tagaactttt agaagttaaa aagtgtatag ttgctttaac taaaagggat
    41101 ttagttgatg aagaatgggc agaaatgata aaagaagata taaagaatta tttaaaatca
    41161 actagtttta aagatgcaac catgatagat gtttcttcta aaactaaaga aggaattaat
    41221 gaattaataa ctgagataga tagtgcagta gaagaaatag agcaaaagga taaggaaggc
    41281 cattttagac tagctgttga tagaagcttt tcagtaagtg gatttggaac agtagctaca
    41341 ggaactatat taagtggaag tgtaaaatta ggcgatttag ttcaaattaa tccaagtgga
    41401 gtagaagcta gagttagaaa tattcaagtt catgatgaga atgttgaaat aggggaagct
    41461 ggtcaaagat gtgcattaaa cttatcagga gttactaaag aagaagtaac tagaggaatg
    41521 gttgtttgta cttctaatac tattgagcca tcttacatgg tagattgcaa gtttagatat
    41581 ctaaagtcca atgaaaaaaa cttagttaat agacagaggg taagaatata tcatggtacg
    41641 tcagagatat ttggaagaat agtcctactt gataaagaag aggtaaagcc tggtgaagag
    41701 gcgtatatac agttaagatt agaaagtgaa atatgcgctc aaaatggtga taacttagtc
    41761 ataagaaatt attctcctat gacaacctta gggggaggta aaataataaa tcccttagca
    41821 aaaaaggcta aaaggtttaa agaggattat ttaaatgaac taaagcttat ggaaaaaggt
    41881 agcatagagg atataattga aaatgtagta ctatctttaa gtgaaacctt cccaaactca
    41941 caagaggtca taaaaggact aggaagaaat atagaaaata tagattttat attggaaaag
    42001 ctttgtgagg ataagaaaat tataggaata gataatggta aatctaaagt atttattcat
    42061 aataaatttt tcactaaaaa gagtgaagaa atttatgaaa ttctaagaag attccatgaa
    42121 gaaaatccat taaaagtagg catgaacaag gaagaactta gaagtaagag tttttcaaat
    42181 aagattaagc aaaaaatatt tcaaagtttc ttagaactta tgaaggaaaa agagatgata
    42241 aaagaaggga ttaatataat ttcactaaag gattttgaga taaaactaac aaaagctcaa
    42301 aatgggatta gggaaaaaat attaaaagct tatttagaaa gtggaatagt ttctccaaag
    42361 ataaaagaca tcattgaaga taaaaagtta gaaaaggagt atataaaaat atataacctt
    42421 ttaattgaag atggaacttt aattaaactt ccagaagatg ttgtaatgca caaagagata
    42481 ttacaagggg ttaaagataa aataatagaa tggttaaata aaaatggttc tattacttta
    42541 ggagaaacta aggagatttt aggagtttct agaaagtatt tagtagcaat tttagagtat
    42601 ttagacaatg aagaaataac taaaagagta gaagataaga gagttttaaa ttaaataaag
    42661 gattctacaa gagaagtttg gtcttatagg cttctctttc ttatattcat aattaaaatg
    42721 taatagtttt gccataataa tacattataa ttaaatagat ttgatttaca taagataata
    42781 aatatagtat atttaattaa tgtatttcag aataatattt aatgctttta ggaggatatt
    42841 atggacgata acaaattcgc taaagagcaa gaaaatgctt taaaaagtga tgaaacatca
    42901 gtaacaaatg agaaaatgga ctcatatgaa aaagaagcag cggtattaga gagtatatca
    42961 ttagatacca atactatggc tattgatgag gaatttatga ataaaccaga taaagacaaa
    43021 aaagatatta atgaaaaagg tgagaaaaac acttctttct ttaaaagaat actagaagga
    43081 attatagatc aaacagtagt attattatta tccttagcag ccttttttat agtagattta
    43141 ataatgagac cttttggcta taagatatct ttagaaggaa gaattccttt ttattttata
    43201 atatttgtta ttatgaacat actttacaga gctataatgg aaggtagaaa gttcaaatta
    43261 acattaggtg aaaaagtttt tagaaaataa ataatttatt agtaagggag acaactaatg
    43321 agaaaaggta atgaaataga attattagta gagggaatag agttccctgc taaaggattc
    43381 ggatttgtag atggattaaa agtttatgct aaaaatactt ttccaggaca aaaagtcttt
    43441 ggtagagtaa ctaagaaaag aaaagagtat gcagaaatta gagtttctga agtaagagaa
    43501 aaagctagtt atgaggaaga ccaaaagtgt ccacactttg gagtatgtgg aggatgttct
    43561 tcccaaacaa ttccttatga gaaacaatta gagcttaagg ttgaacaagt taaagattta
    43621 tttaaagagg ctgaggttcc aacaggagag tttttaggta tagaaactag tccaattcaa
    43681 tgggaatata gaaataagat ggaatacact tttggagatg aagaaaaagg tggagaactt
    43741 acccttggaa tgcatatgaa gggaaaatct tttggaatag taaatgttga taaatgtgta
    43801 ttagttgatg aagattttag aaatatacta gatacaacag ttaaatattt cagagaaaaa
    43861 ggattacctt tctataaaat aatgcctaga acagggtatt taagatacct agtagtaaga
    43921 aaaagtgtta agacagaaga aattcttgtt aatttagtta ctacaactca aatagatttt
    43981 gatttaacag agtttactga gcttataaaa ggattaaact taaaaggaaa attaaaatca
    44041 ataatacata cagaaagtga tgctttatca gaggctgtta taccagagaa agtaaacatt
    44101 ctatatggtg atgattttat aacagaaaat cttttaggat taaactttaa aatatctcca
    44161 ttctcattct tccaaacaaa ttcactagga gctgaaaagc tatatagcat agtgagagat
    44221 tttatgggag ctgctgacaa taaggtagta tttgatcttt attgcggaac aggaacaata
    44281 ggtcaaatag ccgcaggaaa agcttctaag gttattggaa ttgaacttat agaagaagca
    44341 gctatggcag ctaatgagaa tgctaaatta aatggattaa ataattgcaa ttttatagct
    44401 ggagatgtag ctgaggttat aaaaacagtt aaggataaac cagatataat aattttagat
    44461 ccaccaagaa gtggggtaca tccaaaggca ttagactacg ttataaaatt taatgctcca
    44521 caaataatct atgtttcatg taatccaaaa actctagtaa ctgacttaaa agttttagaa
    44581 gctaagggat atgaaataga aaaaacttta cttatggata tgttccctaa cactcctcac
    44641 gtagagacag ttgtaaaatt aaataagatt aaataaatta ttagaatgta taataattta
    44701 tttaatttaa tattctatat tggttatata actggaaatt ttatttccag ttatattttt
    44761 tatatttttt ttcaaacact aataatgaat tagtatattg attttagtat ttacaaattt
    44821 ttgaaatata tactttaaat ccaaatatgt acaagctaaa ttactagtgc agtttcgatt
    44881 ctctcctcaa agattatttc aaattgtagt atgagtcatt tttcttatac cacttgaaaa
    44941 attaaagaat gtacttaatt tatcccagtt agagtaccat gaactataca aacattaggg
    45001 aatacggctt ttatggcttc tagaagccct cttaatccat tcatacttaa taccatgatt
    45061 atttaaatca ttacaaaagc tcaactagaa tttaggtcct tcaacctcac caatccattc
    45121 ttaccaacta aatcttcaac tgatttacaa tttttaattt attctcgata attcatttct
    45181 ttactaattt gaaatccttt ttaaaatatt aagaatatta tctaggttat tatccgaaaa
    45241 aaactttagg gaaatttatt ttacacaaat tttcctacat tctcccaaat ggctacttcg
    45301 gtaaattttc tgcaattata tgtacattta ctgtgcttgc tacaaaatct acgaaaacat
    45361 tcttagagta gtaacattaa tcatagtttt ccaactatta tgacatagtt aaggagaaat
    45421 ctaaaataaa aaatttatat ttcataaaca tttcataaaa ggtgtgtaat atatttttta
    45481 aaccggttat aaatagctat tgtgtaaaaa ttatagtcag taaaaaattt atgaaaaaac
    45541 agtattacgg agagctggtg tattacttat tttatgtttg aaattaaagc gtagttgggt
    45601 gtcaaaaaaa taataaaaac aataaaataa acacttagtc tatggatgaa attagttgtg
    45661 gaataaaaaa atagagagga gcttgttaaa aaagtgataa aaaatgctat agcatatatt
    45721 acaagaaaaa aaaatagaac gtttattatc tttgttattt taacaatagt tttttcttgc
    45781 ttatattcat gtttaagtat aatgaaatca agtgctagct tagaaaagtc tttatataag
    45841 atttctaatt cttctttgtc aataacaaaa aaagatggtg gttattttga tattaaccaa
    45901 tttaaggata tagaaaaaat aaaagaagtt gaagagacca tttttcaata taatggattg
    45961 gcaaaatcaa taaaagctaa agttgttggt ggagagcaaa aaatagagag agataatttg
    46021 cctgatgaat ttaaaaatat tctttctcta gaagctacaa ataaaactaa aagaaatatt
    46081 ttatttaaca gtggagtatt taccattaaa gatggtagaa atattgaaga aaatgataga
    46141 agaaaaattc ttgtacatga agattttgca aaaaaaaata atttgaagtt aaatgatgaa
    46201 attagccttg aattgattga aatggagaac aaggtgcaaa aaaaagaatt taaatttgaa
    46261 attataggga ttttctctgg aaaaaaacag gaacaatata caggattatc ttctgatttc
    46321 agtgaaaaca tggtctttgt tgattatgct acaagtcaag aggcattaaa cagggcagaa
    46381 aataataaaa ttgcaaataa aattttgata ttctcaagca gtgaggagtc cacaaattta
    46441 gctttaaaaa aaataaaaga gcttaaaatt gattggtcaa agcattcaat tgagaaagat
    46501 actaatgcat ttgaagaatc attagaatct gtaagtggga taaaacacat tataaaaata
    46561 atgacttatt ctattatgtt aggtgggata gtggttcttt cattaatatt aattttatgg
    46621 ttaagagaga gaatttacga aataggaata ttattatcaa ttggaataag taagataaaa
    46681 attgtaattc aatttatttt ggaattgata tttatatctc taccatccat aatgtcaact
    46741 ttagtgttag gtaatttatt attaaggcaa attgtagatg ggtttatcag atctgataac
    46801 tcaatgattg ttagcaatag tttacttaat aatagcaatt ctatattaaa tttaggaact
    46861 ttaacacaaa gttatttcat attaataagt attattattt tatcagtaat tgttgcttct
    46921 tcaatgatat tagtaaagaa gccaaaagaa atattatcaa aaataagtta ggaggatata
    46981 atggatatat tagaaattaa aaatttatca tatagttaca caaactctag agaggagatt
    47041 ttatcatcag taaatcaaaa atttgaactt ggaaagtttt atgcaataat aggtaaatca
    47101 ggaacaggaa aatctactct actttcttta ttagctgggt tggataaacc tcaaactgga
    47161 caagttttgt ttaaaaatga aaatatagat aaaaaaggat atagtaatca taggaaaaat
    47221 aatatatctt tagtatttca aaactataat ttaatagatt atttatctcc aattgaaaat
    47281 attagattgg taaataaaaa agcatcagaa aatattctaa tcgaattagg acttgatgaa
    47341 aaacagataa aaagaaatgt aatgaaatta tcaggaggac aacaacaaag agttgctata
    47401 gcaagagctt tagtatcgaa agcacctata attttagcag acgaacctac aggtaatctt
    47461 gatagtgtta ctgctgggga aataatagat atattaaaga cgttagctaa agagagaaat
    47521 aaatgtgtga tagttgtaac acacagtaaa gaggttgcag aggccgcaga cattatttta
    47581 gagttgagtg gtaaaaaact aaaaaaagta agtaaaatta actcggaggt acagtaatga
    47641 taaaaaacgc ttttgcatat gtaactagaa aaagtttaaa gtcagtaata atattattag
    47701 ttgttttagc tatgtcagcc cttagtctga ttagtttatc tattaaagat gctacaaata
    47761 gagcttctga ggaaactttt ggtaatataa caagtagctt ttctatggaa ataaatagac
    47821 aagtaaatcc tggtacacct aggggaggag gtaatgtaaa aggagaagat attaaaaaaa
    47881 tatctgaaaa taaaaacata tattcttatg ttaaaaggat aaatagtgta gctgatttaa
    47941 ttgatcatga cattgtagaa acaaaagaaa cacttgcaaa tcaatcacct gagagatcaa
    48001 aaaactttaa aagaacggtt atgctaacgg gagttaatga atcatcaaaa gaaaacaaat
    48061 ttgtatcggg agcatataaa ttaattgaag gtaaacattt agaaaatcag gataaaaata
    48121 aagttttaat gcacaaagat ttagcaaaaa aaaataatct aaaagttggg gataaaataa
    48181 aaataaaatc taatttattt gatgctgata atgagaaagg tgcaaatgaa acggtagaag
    48241 tagaaataaa aggtctattt gatggacata ataatggtgt tgtaagtgtg ccacaagaac
    48301 tttatgaaaa tactctaata actgatatta atacagctgc taaagtttac ggaaacacag
    48361 aagatacagc agcatatcaa gatgcgactt tttttgtaaa aggtgataag aatttagaac
    48421 atgtaataaa agatatagga aaacttgata taaattggag agaatataat ttgattaaaa
    48481 gttcatcaaa ttaccctgca ttgcaacagt ctatatcagg tatatattca atagctaata
    48541 agttatttgc tggatctttg atatttgcag gtgttgtagt ttcattattg ttatttttat
    48601 ggataaatgc tagaaaaaaa gagatagcag ttttattatc tttaggaatt tcaaaattaa
    48661 caatttttgg acaattctta atagagctga tttttatatc aataccagca tttataggct
    48721 cttatttctt agcaagttat acaggtgata aacttgggaa taatatatta aatagagtaa
    48781 ctggtgatat agctaaaaaa atagcaaaac aatcattatc gagccagctt ggtggagggg
    48841 ctgaagttga tggatttaat aaaactttaa caagtttaga tattaatata ttaccaaagt
    48901 ctatggtgta tgtaattcta ttcatgagtt tagtgcttat tatatctttg attatttcat
    48961 catatagtat tttaaagaaa aatccaaaag aactattaat tgataatgat taaaatagtc
    49021 ggtaccattt ggtaccgata cttttttaac tatttaaaaa aattaaataa gatacaaatc
    49081 gtaaaaaata gctaaagaaa atatatggta tattatatag tataatgaaa taaatttaaa
    49141 aaggaggtag ctatgaaaat attaattgtt gaagatgata aaataataag agaaggaata
    49201 agtgagtatc tatcagaatt tggttatatt attattgagg ctaaagacgg cagagaagct
    49261 ctatctaaat ttaataatga tataaatttg atcattttag atattcagat cccttatata
    49321 aatgggctag atgtgttaaa agaaattagg aagaaaagta agttaccaat tttaatatta
    49381 acagcattta gtgatgaaga atataagatt gatgcattca ctaattttgc agatggctat
    49441 gttgaaaagc ctttttcttt acctgtatta aaggctagaa ttgattcttt aattaaaaag
    49501 aactttggac agatagagaa gttcgaatat aaaaatatag atgttaattt taacagttac
    49561 actgtaaaaa taaatggtga gaatatatat atatatgcaa aagagttaga aatattaaaa
    49621 tacttagtag ataatgaagg acaagtatta acgagaatgc aaataattga taacgtttgg
    49681 aaggaaacag aagaaattcc ctatgatcgt gtgatagatg tatatataaa agagctaaga
    49741 aaaaaattga aattagattg cattactacc attaggaatg taggatataa actggagaga
    49801 aaatgaaaga tttaaaaata tttcctaaaa tttttataca aacattctct gttattggaa
    49861 taataattat actaatgcat ttgttaatat tcttaatttt ccctaggaca tatttagata
    49921 ccagaaaagc agaaatttat aataaggcaa atgaaatttc taggaatatg aatggaaaag
    49981 aattaaatta catagagcaa actttagatt tttattctaa tacaggtgat ataaaggcat
    50041 ttataaaaga tgtgaacgac aataatgaag taaaaataaa aaaacaagtg aatattaatt
    50101 tagaaagcaa caataattct ttgataattg aagaaaggga aataacgatt aataatggta
    50161 aaaaaatata tctacaattt gtttctatag ctgatatgca aaaagatgca aaaaatttaa
    50221 gtcttaaatt tttaccatat tcattattaa tatcactttt attttcgtca atcatttctt
    50281 tagcttatgc taagtcaata aagaataaca tacaggaaat aaaaaatgtt acagataaaa
    50341 tgatgaaact tgataaaaaa gcacgtttaa agcttgactc taatgatgaa gttggacaat
    50401 taaaagaaca aattaatgat ttatatacta cattattaag aacaattgat gatttgaaat
    50461 ttaagaacaa agaaatatta aaactggaaa agttaaaata taattttttt aaaggagcat
    50521 ctcatgaact taaaactcct cttgctagtc ttaaaataat actagagaac atgaaatata
    50581 atattggaaa atataaagaa agagatattt atattaacga atgtattgat attgttgata
    50641 gtttaacaaa aaatatttct caaatactat cagttcattc catagaaaac ttgaataatg
    50701 atgaagaata tttaaaaata aatgatgtat tagaaggcgt attaaaaaag tatgaagtat
    50761 tagtaaatca aaagaatata actataaata actatatatt agacgagaat atttatatag
    50821 gaaaaacggc tttaaatatc attctatcta atttgattag taatgcagta aaatatactg
    50881 atgcaaatgg tattattaat attggattag atagagattg gttatacatt gaaaattcat
    50941 atggagataa taaagtttct gatatagata aattatttga gtttaagttt gatttgaata
    51001 aggaaaatag taatggatta ggattatata ttgtaagcaa tcttttgaat aactataata
    51061 aaaaatttaa agtattacaa aatagagagt attttgtatt taaaataaaa ttatcttctt
    51121 aaacaataac aaattatata acaatctgtt ctgtaaaaca agtagtgata atgatacttc
    51181 aatagtttaa tttgtgctat ttttaattat taagaagcat taaagattct tttatttaat
    51241 tatgaagtaa aggaatcttt aatgctaata tttcaagtgt ttataatcta tctaaatatt
    51301 acttttaaaa ataaaattat agatgctaaa cataatatta aacataataa agttccaatt
    51361 tttataagag cataagataa actagtatct atcaatattg gaactaaaaa aactaaaagt
    51421 gaagtgattt ataattatta ataattatat ttgtttattc catatctctt tattttatta
    51481 tatagagcat ttctactaat accaagagca ttagaagctt tagttatgtt tccatcaaat
    51541 gtacataaag ctttttctat agcttctttt tctaaaatta ttagaggttt aattgaattt
    51601 tcattataac tttcaggtaa atctaatgaa ttcgggttaa taggattacc taagttatct
    51661 gttttaagac aatgacattc atcaaaatta atttcataag aagtagaacc atttagatta
    51721 actatatttt caacacaatt ttcaagttct cttatattac caggccaaca ataagaaatc
    51781 atctttttat agagtttttg atttatatta ggaatacctt tgtgcaattt ttggcttttt
    51841 atatttaaaa aatagtttat taaaatagga acatctccta atctatcctt taaaggagga
    51901 agatgaagtg gcattacgtt taatctatag tataaatctt ctctgaaagt tccttttttt
    51961 atttcttctt ttaaattttt atttgtagca gctataattc ttacgtttat aggtatttga
    52021 gtttttccac ctattcttgt tactactcct tcttgaataa ctctaagaag tgttacttgc
    52081 atttctattg gcatttcgca tatttcatct aaaaatatag ttccaccatc tgctaattca
    52141 aatttacctg gttttccacc ttttctagaa cctgtaaatg aggcttcttc ataaccaaaa
    52201 agttcacttt ctattaaagt tttaggtatg gcaccacaat tcaatgcaat aaaaggacca
    52261 tttgacacat tgctagcgtt atgtattgat tgagcaagta attcttttcc acaaccactt
    52321 tcaccagtaa taagaacagt tgatggactt tttgaaataa gctcagcatc tcttaatact
    52381 ttttttaact gtggactttc acctattata tcatcaaatg tataagcagc ctcttggtta
    52441 gaatatttat ttacagtact tattatttct tttaagtctt ttagtattaa aagccaacct
    52501 acaaatatat ctccatcatt tatagggcaa atatcaagat tatatttgtt accatctaaa
    52561 gaagttacaa gtgcatcttt ttgattgttc ttttttgaag aggaattatt aataagctca
    52621 tttaattgag gaaaaaatga aaatatatct atattttcaa ctgatttaga tttactattt
    52681 aaaaaagatt cagctttttt gttaatgaaa tctattttat tttctggtga tataaaaatt
    52741 aatccaagtg gaatgtgatt tgtcaaattt tctaataaaa ataatttttc aaaatttata
    52801 tccataaata ctcctttctt tcaaataatt cttataaagt tatttgaatt ttatacaata
    52861 aaaataaatt ttaataaatt tactaagcta ttatgaagtt atcatttgaa attaatttta
    52921 acatattact ttaaaagata aaattaaaaa aactattgat aaaaatatgg caaataataa
    52981 ataaaatggg atatcaaaag tgtacaaaaa gatgacagtg ttcaaaaaag atacaaaaat
    53041 atgagcaaaa tgattactaa aacttatatt aaaagtattt taaataaata aaaaattaac
    53101 aaactcaatg ttttcagtag taaaaatata tttggcatga aaattgctaa gatataaaat
    53161 agttgaaagt ttaaaagtga aatttataac aaataagaaa ggaatgtaat tatggaagta
    53221 aacaaaagaa aatttcaaaa aggtcttatg tttggtattt tagttggtct tgcttgggga
    53281 cttgatggag tattaatggg aagagtaggt atggataaaa tatttacaga caaagctttt
    53341 gctttagcac aaggtgtttc agaaataagt tttgaattct caccattagt aactgcattc
    53401 ttccacgaaa gtttttgttt cgtttgggtt gcattggtat taatatttgg aaaacaattt
    53461 aaacatgtat tccatttact atttaaaact aaaaaaggta aagcagcggc tttagcagct
    53521 ttagttggtt caccaatagg tatgagtgct tacttattag gtattaaata tgctacagca
    53581 ccatatgcgt caagtatttc agttatttat cctggagtgg gagcgatatt atcatatttt
    53641 atacttaaag aaaaattatc actaagagca gtaataggta tagcagttag tttattagga
    53701 tcattcatgt taggatttaa tccttcaggt aatgtgccac caactttctt taaaggtata
    53761 ttatttgcag tagtagctgt atttggatgg gctttagaag gtgttatcat aggatttgct
    53821 atgaagcata taaaaggtga agatcatata caagcatcac cacaacaatt tttatgttta
    53881 agatatttta tttcaatggt ttcatatgca gtaatagtat taccatttat aggtggatat
    53941 ccactagcag gtcaaatagt ttcaagagga ttagtattta aatatgcagg aattgccata
    54001 ttaggagcta taacttatct ttcatggtat aaagctgttg atttaatagg agcagcaatg
    54061 ggtactgcat taaatagtac agcagcatta tggacaataa tattcagtgc attattattt
    54121 ggggataaaa taactccatc attagcaata tggggaatag taatagtttt aggtgtcttc
    54181 atatttgcaa tagatccaaa gacattaaga aaaaaagctt aattttaaaa aagtaaaaaa
    54241 caaaataaat aaatataata attaatttaa aatcaataag ggtttagaaa tttaattttt
    54301 attaaattct atccctttga ttaatatata gacattgaag aatttttaat aaaacattat
    54361 aagataaaaa ttagtacgat ataggaggaa tggcaatgtt aaaaaaagaa atcgtatcta
    54421 atatggaaaa gatagttgaa aattgtatgg gggaagaaac agccgcttgt gttgcaactt
    54481 gtcctatgca tactgacgtt aaagaatacg ttagattaat aaaagaagaa aaaggcgagg
    54541 aagctataaa agttataaga gaaaaattat tcataccagg aacattagga agaatatgtg
    54601 cacatccatg tgaaaagcaa tgtaaatgga atgaaggaaa aagtccaatg gcaatagctt
    54661 cattaaaaag atatgcagca gataactttg ataaagaaga gaattgggat ctttcaataa
    54721 aaaatagtaa tgggaagaaa attgccgtta taggtgctgg cccttctgga ttacaatcag
    54781 ctttagatct aagaaaagaa ggattcgaag ttacagtatt tgaaaaacta cctgttagag
    54841 gtggaatgat gagagtaggt attcctgctt atagattacc aagaacagga ttagagcatg
    54901 agataagtta tctagataaa ttaggggtta actttgaatt aaattgtgaa atcggaaaag
    54961 ataaagagtt taatgatatt gttaatgaat ttgacagtgt ggttgtcgct gttggtaaac
    55021 atcaaggaag agttgataga agtttagaaa attttgatgc tgaaggtata tttagtgctg
    55081 ctggatattt aaaagaagca tctttaactg gaaatgtaga aaaggctggt aaagtagtat
    55141 tagtagtagg tggcggagat gttgctatgg actgtgctag aacttcatta agaatagatg
    55201 gtgttgagaa agtttattca atatgtttag aagatagctt tgataatatg gcatcatcaa
    55261 atcatgaaat agaaggtgct atagcagaag gaataaaatt caatcatgcc caagctataa
    55321 aaaatatcca tatagatgaa aatggaagag tttcaagagt aacattaaaa aaatgtcttt
    55381 caatgtttga cgagaatgga agatttgctc caaaatttga tgatgaagct acaagtgaaa
    55441 tagaagtaga tacaatagta tttgctatag gtcaaggtgt tgagggagaa tttgctaaag
    55501 atatattagt tcaaagacca aacactacat ttgaatgtga taaagaaaca ttacaatcat
    55561 cttcaaatga gaaagtattc atagtgggag atgcttcagg ggaatcagta atagttatcc
    55621 aagctatggc tactggtaga agagctgctc aatctgttat aagattttta aatggagaaa
    55681 gcttaacaga aggcagagag ttaaaggata cttggactta tgaaactaag ttacaaatgc
    55741 cgactgattg gtcatcaata actggtaaaa gagaagatat ggaagagtta gatccagcag
    55801 agagaataaa atcatttgat gaagttgctt taggatatac taaagaggaa gctttaagag
    55861 aagcagatag atgtagacaa tgtcaatgta agctttgtat gaaggaatgt ataatgctta
    55921 atgactatac agattgtcca aaagctttat tcagagaata tctagaaaaa ggttatgaaa
    55981 acatggataa gatgatagca tattcatgta atgaatgtaa tcaatgtaca ttaaaatgtc
    56041 caaaagagtt agatttaaaa gttaacttta gagctatgaa agaagctttt tctgaagaaa
    56101 atggtgggtt agcaccatta gaagcattaa aagcaagtga tgctactcaa gaaaaagagt
    56161 gtgcagaaga atactgtaca acagttgaag cagcctcagt tgaggaagtg aaagaaaaga
    56221 aaaaagctaa gaaaaaaacc aagtatgtat ttgtaccagg atgtacagta ccagcttata
    56281 ctccaggggg agtagaaagt gttttaagac acttaaaaga ttctttagga gaggaaaatg
    56341 ttggtgcatt acttcaatgt tgcggtaaag taacttactt aatcggagaa gaagaaaaat
    56401 acgaagaaag aaataaaaag gctatagata tattagatga gatgggagca gaagttataa
    56461 taactgtttg tccatcatgt tataaagtat ttaaagaaac agctaagaat caaagagtta
    56521 tagcatactg ggatttaatg aaatatttaa taggaattcc agcagagtct aaaggaatag
    56581 gagaaggttc agacgttata tttaacatac atgattcatg tgtaactaga gatgtaactt
    56641 cacatcatga aagtgtaaga tggatcttag accaattagg atataattgg gaagaagttg
    56701 aaagaaacgg taaaaacact agatgttgtg gtgttggagg aatggtatgt agctcaaacc
    56761 cagaattata cgagagagta tacactagaa gagctaatga ttttaaccaa gacaacatag
    56821 taacttactg tggttcatgt agaggaacta tgcaagcttc tggcaaagat gcagttcata
    56881 tattagatct tatctttgga tcaaaatata ctaaagatca agctcagcaa agaggatata
    56941 ggacagaaga agaaatgtgg gctaatagat tagaaactaa agaaagacta aataaattta
    57001 aaaagtagtt tttttagaat attattatcg atttaaatac aaaaacacat aaatagtatt
    57061 atgactattt atgtgttttt atttattttg tatagatatg taggagtatt tttagtaatt
    57121 aacagaagat aaaaatagtt ataagattaa gcttagagat aattcgttat tagtgaaatt
    57181 agagttgtta tttgtaacat tatttaaatt atagagtaaa ttaaaataag agttaatttt
    57241 aagaggtgag gagccataga attagtacaa tgtccaaatt tttttagtga agattctcca
    57301 aattatttag ttgaatatag aggaaattta aaagaagagt taaaaaaaat tccttatgca
    57361 tgtggagata caattactga taccttaggt gttgtttcaa caccaccaca gtatttgaaa
    57421 caactaagaa aagatgttcc atcaattcgt tatatacaga caagagtgcc ttatgtttta
    57481 caggaagtat ctccacaaga aactgataat atatccacaa ttgtaaataa tccatattta
    57541 gatcttgatg gaaatggagt tatagttgga atagttgata ccggaataga ttatcttaat
    57601 aaggaattta tgagagaaga tgatacctct agaatactta ctatttggga tcaaaagagt
    57661 actaaaaaac ctaatgattc agtttatgta ggatctatat ttaataatga agacataaat
    57721 aatgctatta aaagtaaggc agatgggaga gatccatatg atattgttga tagtagagat
    57781 gaaatatggc atggaactaa attagctagc ataataggag cacgtggata taatagaaaa
    57841 attaaaggta ttgcacctaa ttgtgatttt gctattgtaa aactattaaa ttcatttagt
    57901 tatgaaaaag cttttagaga aaatggaata gaaaatgttc cagtttatga tgaagttgaa
    57961 attgtagcag gagtagaata tctcaaaaat tatgccctca gtcttaagag accattagta
    58021 atttgtttag cgatagggtg tactgaagca agtcatgatg gaagaggact ttttcctagg
    58081 tatttaacta cagttgcttc aataagggga attgctatag ttgctggagt aggaaatgaa
    58141 ggaagtgcac aagggcatgc ctctggagtt atagaacttg aaaatagtgt tgaaaaaatt
    58201 gaattatcaa tacctagaga gataaaaaat tttaacttag ctttctggat tcaaagacct
    58261 aatataatgt ctttaaatat aaaatctcct agtggagaag aatcttcatt catagatgct
    58321 aaaatttttc tagagagatc atttaaattt atattaactg atacaagtgt taatattaac
    58381 tattatgttc ctgatacctt tacaggaaat gagcttattt atgtagagtt taaagatatt
    58441 aagcctggaa tatggacttt tgagttaaga ggagattata taactaatgg aagatatgat
    58501 gtatggttag ctccaagttc tttattgcct gaaaatacaa aatttcttag ccctaatcca
    58561 ttaaatactc ttatggatcc atctactgct aaatatataa taaccgtagc ctattataat
    58621 agtcaaactc aatctttatt agcggaatct ggaaaaggtt ttaatgttaa tggatggata
    58681 aatccagata taaccactgc aggtaaagat attttgacca tatttcctgg agatagagtt
    58741 ggaagaatgt cagggagttc tccagcaaca gcaataactg taggagtatg tgccttatta
    58801 tttcaatggg gaattataaa tagaaatgat agaacaatga attctagtaa attaagaagc
    58861 tatttaatat atggggctac tagaattcct gggcaaacat atcctaatga atatactgga
    58921 tatgggtatt tagatttata tgaaatattt agaaatataa taggagttcc tttaccacct
    58981 tatagaagta ctaagattaa tgaagattat actgagtatt attgtggaag gatgttagtt
    59041 agcgtgcctt ctgattttta ttatggaggg aaataaaatg gaatttaagt cttcagctcc
    59101 agaatatgta tttaataatg aaaattattt aaattatcta gttcagtatc aaggtgacat
    59161 tataggagag tttaataatc aaaatggaat atatgcaact ttaattaatg atagatatgc
    59221 cataataact attgataaaa atttacagga atatgaaaat gatatatcta tgattaaata
    59281 taaaaaagaa aatggaagag atgtagaaat agataacatt acttatataa aagatccaga
    59341 aggatatgta cttcaggaaa taagcccttt acaagcagca aatattgaat atgttcaaat
    59401 acaatcatac tttaatctaa caggtaaagg ggttattgtt ggaattttag atactggaat
    59461 agattattta aacgaagagt ttatggattc atatggtaat acaagaattt taggaatttg
    59521 ggatcaaaca atttcaagtg aaagttcaag ggagaataat ttaccttatg gaacttttta
    59581 ttcagaagaa gatataaata gagcaataag acttagtaga gaaggaggag atccatatac
    59641 aatagttcca tcaagagatg aaataggcca tggaacatca atggcaggaa taattggatc
    59701 atcaggaaaa aatcctaggt taaagggggt tgcaccagat tgtaagttct tagttgtaaa
    59761 gcttgcacag tcactttatt ataaaaaaga gtatgaaata aaaatacctg tttataatat
    59821 aactgaaata tttacaggaa tacaatattt atattcatac tttttaaaaa gatctcaaag
    59881 tatgattata tatttacctt taggcactaa tagaggaagt cataaaggaa caagcatctt
    59941 agaagaattt ttagattcta tattaataaa tagaggaatt gctttaatta caggtgctgg
    60001 aaatgaagga gcagcattat tacatggatc aggaactata aaacctaatg gacaagtaac
    60061 tactcatgaa tttaatatag atgaaaatca aaaaaagatt atcattgaag cttggataca
    60121 aatacctagt attgcttccg tagaaatagt ttcacctact ggaggaacta caggaataat
    60181 tcaacctttc tttggaaaag gaaataagta ttattttaca attgaacgaa ctacagtgtt
    60241 agtaagttat tatatacctg aagaaattta tgaagattca ttgatattaa ttatactaga
    60301 taatgttcaa gctggaatat ggagttttaa gtttagagga ttaaataata tagaaggaag
    60361 atataatatt tggcttccgc caaaagggat aagtaaagaa aaaactagaa tgatatatcc
    60421 tgacccatat ggaacagtaa ctgtcccagg tactagcatc tctgtaataa cagttgctgc
    60481 atataatcaa ttaaataata cacaattaat ttattcaggg agaggattta aagataatta
    60541 tattgatatt atagatgtag cagcaggagg agtaaatgca cttacagttg cgccagataa
    60601 taagacaact ttagcaaatg gtacaagtgt tgcagcggct atagtagctg gaatttgtgt
    60661 tttacttttc caatggggaa ttgttgaagg aaattatcct tatatgttct ctcaaacttt
    60721 aaaagctttt attacaagag gtacaagaaa gagaaaaggg gacacttatc caaatccaga
    60781 gtggggatat ggaatagtag atatgtttaa tatgtttaat cttactaatt aaatctttta
    60841 ttatataaaa tatattttga aaccttatta tatttaatta gatagaaaaa ataaaaaact
    60901 ctgattaaaa ttgtttataa ctaattttaa tcagagtttt aactttaaaa cttaaaacta
    60961 ttttaaagtt tcttttatac tatcatctac agaggatatt gcggtttctg cattaatttc
    61021 aattacctca gaatcattat tagttctatt atattttttg gtaaatatta taagaagaaa
    61081 tatgctagct attaagtaaa gagcacctgc tatatagtaa gcttttccat aacctgattc
    61141 tgtctttaat aaagaatatc ttgtatatat accagctaca attcctataa gtgaattaac
    61201 aactaatata agtgaattat aagctgaaac taaattttta ggaacaaagg ttaatggtaa
    61261 agaactttgt attggagcag aaacatttgc tacaccagat cttaaaaata gtattatacc
    61321 tactatcata gcaacattac ttccaaatat agcaccatta gccattaata tcataagtgg
    61381 tacacaaagt atagtagtta cagatatggt tactattgaa ccaaattttt tttctaggta
    61441 aggggtgcag aaataaccaa ttaccataga tattgtttgg aatgttatga tagatgaaac
    61501 agtacctctg cttatatgta agaaattatt taaataaatt ggaaagaatg gtgttaccaa
    61561 taaagcacca aatcttacta ttccaaatat aagaatccat aagataacat atttattagc
    61621 taataatttg aaatcaaaga ctttttctcc ttttctagct tgaatttctt caggagtttc
    61681 tctataatct tcactttttt cttttaagaa taatagaaag actgaagcta ttactgttaa
    61741 ggctattgca atccaaagta caaatttata tgaatctgaa tatgccatta attgaacaga
    61801 acttaaggca tcttgatttc ctgataaaac agaagctcta ttataagaga tacctaaaga
    61861 tttagcaaat ttccaaacaa caattttacc atttaaaaat gttaaaattg attggctaat
    61921 aagattgcag tacataaccc tagagaacat tttagttctt tcttttctag gtgtatatga
    61981 tgtaaacatc acaggataaa tacaatcaaa cattcccaaa ccaatattta caactatata
    62041 tgcaaaagtt atgattaaac catctttact atatatacaa accaataaac caagaattga
    62101 aaaaataggt gcaagtaata ttaatttttt aaatcctact ttatgagtta atagtaaaag
    62161 cattgctatt atgaaggttc caattgatgc ataggtagga agtgctttaa ctacattagg
    62221 aacagtgatg tttaaataag ataaataaga gtcattagtt attcctgtga cagcccccat
    62281 gaaaatataa cataatataa acattgcaca gttatacttc catcttttgt cccagttttc
    62341 atctttgctt gtaattagat taactagacc agcttccata taaattcccc caattttata
    62401 aatttgttta aattttcaat ttaataataa ctctttataa ataaaaaaat caaaattata
    62461 aaaaaatata ttaagaaaaa aatatataga ataattatat aaataataat tatataagta
    62521 ataaattaac atatataaaa aattaataag tttttaaaat aaaattaaca tttatttggt
    62581 attaatgaga aaaatcattt ttaaaatata aattttggtg taatatataa gaaaagtaat
    62641 gtaactgtaa acataggagg aaagagtatg ataaagagta ttgataatat agaattaaag
    62701 aaagaattag catataatct aatgaatgat tttaagaatg aactttataa taaagatatt
    62761 attgagagaa ttataaataa aatagaacaa attccatttt atgtttatat agaaaaagga
    62821 aatgaaatag gatttctttc aatgaaatct ttaactgagg agtcagcaga tatttatttc
    62881 ataaaaatgt tagatgaata taaagagaag ggatttgagg gggagcttat aaataatgta
    62941 gcaacctttt acaaggaaaa aggttataag cttttaagag ttaagatatt agcagatggt
    63001 aaacatgata acttaataaa tttttatagg tcagttggat tcttcaagtt agaagaaaga
    63061 atagaatcag gagatataga aaataaagtt ttggttatga taagaccatt aatttagaat
    63121 ttataaataa gcatatatta caaatattat taatata
//
LOCUS       DAEVGI010000004        62741 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEVGI010000004 DAEVGI010000000
VERSION     DAEVGI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 62741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62741
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.004"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            105..1832
                     /locus_tag="K8M77_000212"
     CDS             105..1832
                     /locus_tag="K8M77_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBI7084742.1"
                     /translation="MYREKYEEWLNSDIISEEIKAELRDVKEDKEIEDRFYKELDFGT
                     GGLRGVIGAGTNRMNIYTVGKATQGFADYLNDNYAGEKSVAIAYDSRNMSKEFAKAAA
                     LTLCANGIKVNLFESLRPTPMLSFAVRELNCKGGIVITASHNPKQYNGYKVYGDDGCQ
                     LTDAPAKAVIGYVNKVTDYANIKTMSEEKALEEGLLVYIGEEIDKKYIDDLKTLTIRE
                     DLVKKHAKDLKIIYTPIHGSGNVPVRRILKELGYENVFVVKEQEMPDGNFPTAPYPNP
                     EDPKVFKLALDMAKEIQPDIIFGTDPDCDRIGVVVKDNNGEYQVLSGNQTGMLLTNYI
                     LSSLKEMNKLPKNGVVIKTIVTTESVRKMTEEYGVTLIDTLTGFKYIGEKIREFEESG
                     SNEYLFGFEESYGYLAGTFARDKDAVVASMLIAEMTLYYKEQGKTLYDGLIELYNKYG
                     YFKESLVSIELAGKEGQEQIAKCIDGLRNDALKEMNGVKVITSFDYKLSKEVNNLTGE
                     EKEVKLPKSNVLKYVLEDDSWFVVRPSGTEPKMKIYLSVKGSSLEDSKEKTENFKNAI
                     MEVINAKLK"
     gene            2124..3221
                     /locus_tag="K8M77_000213"
     CDS             2124..3221
                     /locus_tag="K8M77_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084743.1"
                     /translation="MSFNTFAKEKLSQLLFLEIDGDGFVKSLGKDPKEVNINEVYIPI
                     DPKHLSQDVKSGYKLESLPINYLVEGMFFALGGDKNFKFNKEYKKLIPLIEDAIPCVK
                     KIVADKVKEENMIEAFMLLKGLSEISDETEVYENLLLICESLRERNKAYNETQLEICD
                     KCKANRSDLALPYLYSAIAYNDIGQYDKAYVDINEYLAKGGERNEIVEVLYNEIKDSA
                     DYEEGKEDLIEEPEDALKRLLPLADKFQDNAILRYYIATAYRRLGNFEKAVYYLNECL
                     SIDDSIVEAVNEMGINYASLGIYDEAIKYLRKAFESTRDIEVCTNLIVCYLNAGKIEE
                     AKQHLDIAKAINKDDEIVKEIETFMKNNNIK"
     gene            3237..3953
                     /locus_tag="K8M77_000214"
     CDS             3237..3953
                     /locus_tag="K8M77_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HBI7084744.1"
                     /translation="MYKELLGYKIFSDNKEELLKEIENKKRVNIISGNPEVLYNGIKN
                     EFLKNSFTKEDALIIPDGVGTLLAAKINGIDITEKIAGIEVMNMLLEEARDKNLKVFL
                     LGAKEEILIKCKEKIKESYDGIDIVGSNNGFFDLDNCDDLIEKINESKADILFVAMGA
                     PRQEVFIEKYKDKLCCKIFMGVGGSFDVFAGNVNRAPQFMINIGMEWLYRVAKEPWRI
                     KRLGSIPKFLVLSIKERGKR"
     gene            3953..5239
                     /locus_tag="K8M77_000215"
     CDS             3953..5239
                     /locus_tag="K8M77_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI7084745.1"
                     /translation="MKILFIACYSPMINNSASIETLMYLNNLCNIENNCVHLLTVDFP
                     KNSIYYDEEILKLLDSKVKVHAIEGGKLFNKIMPKKSIGAKEDEKSSNTKSSSKIKLM
                     RKIKNKIIFPDMYYNWSFKASKYGIELMNKEKFDVIFSMHEPPSSHLCALRIKKHFKE
                     IPWVLYWSDPWLKDPSRENIGFIRKFIEGRQEKSVVLNGDRHIFVTEENKKDFMEKYN
                     VKEDKMFIVTRGYNKAIYEEIERAEKPELLKNNKINLIYAGEIFSKIRDLKPFIKALK
                     ELEKRDQELFNRLNIIFFGNIDDENIKEELKKFSNVSVNGRIDYKEALRYMIHGDVLL
                     VLGNKNSKQIPAKIYDYLGTKNLIMVILGDENDPIKNVALNKEKCIVSENNYEAIIDD
                     LNKCRDLIDSGKKFKANEEYEWSSIGKRLNNILKLK"
     gene            5268..5903
                     /locus_tag="K8M77_000216"
     CDS             5268..5903
                     /locus_tag="K8M77_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBI7084746.1"
                     /translation="MKNEKIKDFIEDLGSSSSAPGGGAAAGIVGAVGVALTSMVYSLT
                     VGKKAYEALSEENKKKLDENLENAKKSYNEMLDFMNKDEEAFTALMDCYKLPKETCEE
                     KSLRSEKIDECTLGAMMVPLELSRKALKFFDNVKFASKYGNKNLISDAVVSAIMLSAC
                     IDSSIVNVEINLGFLKDKNLVKSVKEEILHIRNESNKLKEEILKESGFLQN"
     gene            complement(6085..6882)
                     /gene="pdaA"
                     /locus_tag="K8M77_000217"
     CDS             complement(6085..6882)
                     /gene="pdaA"
                     /locus_tag="K8M77_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBI7084747.1"
                     /translation="MIKKVFSLLFSIMMILSITLNTKVSAKEFNSPCTKEFNWYYSFN
                     KEEGIPHGPKETPYISNHKVVYCGDTSKKEIFLTFDEGYENGNTAKILDILKENQVPA
                     AFFVTRPYIKDYPDLIKRMDEEGHLVCNHTSHHPSMAKILDKEKFTKEFTEVEEEYSK
                     VTGKEMPKFFRPPMGKYSEQSLAYTEELGYTSVFWSFAYKDWLVDEQPSKEAAKKKIL
                     DKVHNGEVALLHAVSDTNTAILDEILKELKSNGYEFKSLNDLTPKKE"
     gene            7041..8138
                     /gene="ychF"
                     /locus_tag="K8M77_000218"
     CDS             7041..8138
                     /gene="ychF"
                     /locus_tag="K8M77_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBI7084748.1"
                     /translation="MKLGMVGLPNVGKSTLFNAITKAGAESANYPFCTIEPNVGVVSV
                     PDKRLDVLEKIYNTKKKVYTAIEFYDIAGLVKGASKGEGLGNKFLSHIREVAAIVHVV
                     RCFDDENVVHVEGSVDPIRDIETINLELIFADLDVLERRMEKTMKLVRSGDKTAKFEY
                     DVMERLKAHLEANKPARTLEATEDEEAFVKSLFLITSKPVLYACNISEDDMMEGNLDN
                     EYVKKVRAYAETENSGIMVVCAKLEEELSGLDEEEKAEMLSEYGLEESGLDKLIQASY
                     KLLGLMSYLTAGVQEVRAWTIKQGTKAPQAAGKIHSDIERGFIRAEVVSYDDLVECGS
                     EAAAKEKGVYRLEGKEYVMKDGDIVNFRFNV"
     gene            complement(8230..8326)
                     /locus_tag="K8M77_000219"
     tRNA            complement(8230..8326)
                     /locus_tag="K8M77_000219"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8290..8292),aa:Sec,seq:tca)
     gene            8684..9616
                     /gene="rsmH"
                     /locus_tag="K8M77_000220"
     CDS             8684..9616
                     /gene="rsmH"
                     /locus_tag="K8M77_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBI7084749.1"
                     /translation="MEFKHVSVLLDECINALNIKEDGIYVDCTLGGAGHSSEIVKRLS
                     SDGRLIGFDQDKDALKAAGERLKDYKNVTYVHSNFYAIYDVLTDLGIDGVDGILMDLG
                     VSSYQLDNGERGFSYMQDAPLDMRMNRENEFSAYEIVNTYSEEELYRIIKEYGEEKFA
                     KRIASFIVKNREEKNIETTLELVEIIKAAIPAKARREGPHPAKRTFQAIRIEVNKELE
                     IISKTILDGVKKLNKGGRMAIITFHSLEDRIVKNTFKELANPCTCPSEFPVCVCNRKP
                     EVKLISRKPIEASKEELEFNPRSRSAKLRIIEKL"
     gene            9689..10117
                     /locus_tag="K8M77_000221"
     CDS             9689..10117
                     /locus_tag="K8M77_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBI7084750.1"
                     /translation="MIVKEFDYNGSSALKPQRKEQTIPSKETYDKKKKKIEQQRRKKQ
                     KDKKIRKSVYQIVLLILVVGGITISRDVKVYKTQQQLDSLNKEINSVITENEALKVQI
                     LKASSLDKIEETAKNKLKMILPTKENMIKLGESQATEGKQ"
     gene            10305..12524
                     /locus_tag="K8M77_000222"
     CDS             10305..12524
                     /locus_tag="K8M77_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBI7084751.1"
                     /translation="MKNKKNKGFKDYKITKSLRFKRTYWTMIVVWGLLGVLTLRLSYI
                     MIFKHKEYGSMAEEQWKHEIKIGAKRGEILDRNGAQLAVSANVYRVDLDLKTLREDTF
                     TKDDTDDTKKEKLNKIAGELGTALGMPKEEVYDKINSTFPSGLPATSVTLIRKIEKDK
                     ADSVKNLKIRGVIVSQDTKRYYPDNNFLAQVLGRVDADGIGQGGIEREYNVELSGLPG
                     MRISEVARNSSGIPYSNSEFAKPVDGKDVTLTVDETIQYFAEKVAEEGKKEYKADGVS
                     IIVMNPKNGEILAMANKPDYNPNEPYKGYENFPGKDKTEKMENMWKNDAVSNSFEPGS
                     IFKVVTSSAAVQEGIAGGNETYFCPGGKNVSGTYIKCWKPDGHGTETFDQILENSCNV
                     GFMDIGQKLGKEKLNEYIEKFGFGKQTGIDLPGETTGIVLPNDKIGPVELATISFGQT
                     DSASSVQMMAAMNTIANGGTWIQPHIMKEISHEDPSGARVVDKTFVPKKINNIIDKKT
                     AMRVSEALEKTVHFGSPKRAYIEGYGIAGKTGTAEKVKASGGYGAGYVASFAGFAPYN
                     DPQVSILISVDNPKGEYFGGLVAAPLAHDLFSDIFNYIELDSSDLDKNKVKEEIVPDV
                     RGMNLDKAKSILDKDNIKYSVEDAGNSVVDMNPKPGYTIKEGDEIKLYTKTTSNYNKD
                     VVVPDFNGLSMEKAKEILNKIGLKGIFAGEGVIKEQSVAQGDVVKSGTSIEFKLDKK"
     gene            12619..14073
                     /locus_tag="K8M77_000223"
     CDS             12619..14073
                     /locus_tag="K8M77_000223"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBI7084752.1"
                     /translation="MILKSLLKGLDYEVIKGNEESKVQNIRYDNRKIEQGDAFVCVKG
                     FKVDGHSFIGDAIKKGAKTLIVQEDVSVQEDITIIKVRDTRKALAIMSSNYFGNPKDK
                     LKIIGITGTNGKTTSAFIIKSILEKAGFMTGLIGTIANYIGNKKVDAVRTTPESYELH
                     ELFKNMVDAGVEYCVMEVSSHSLELDRVYGIQFEEGIFTNLTRDHLDFHKTFENYYNA
                     KFKLFERSNHSIINLDDPYGANIVKDIEERGVKTKVSTFSIEKESDFKAFEIKSHSNG
                     SEFKVNLEEVEDFSINIPGEYNVYNSLGCIICAYNLNIPMNKIKEGLSDVVIPGRCEL
                     VAKEKNLPYSIIIDYAHTPDGLENILSTVKAFTRNRMISVFGCGGDRDKVKRPQMGKI
                     GCELSDIAIITSDNPRSEEPMDIINDIVKPLNYDNFVIEVNRKEAIRKAMNMALEGDV
                     IVIAGKGHETYQILKDETIHFDEREVVYDILEGK"
     gene            14086..15447
                     /locus_tag="K8M77_000224"
     CDS             14086..15447
                     /locus_tag="K8M77_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBI7084753.1"
                     /translation="MLELNLQEIVKATKGALLKEAYVKEIKAVSTDTRKIEEGTMFIA
                     LKGENFNGNNYVLEAFNKGAKIAIVDEVKCDLNELKEDVALIKVQNTRRALMDLAKFY
                     REKLGLKVVGITGSAGKTSTKDLVAAVLSDKYKVFKTKGNFNNEIGLPLMILELDSTY
                     DVAILEMGMRGLGQIKELAEIASPDFGIITNIGISHIEILKTRENILKAKMEIATFFD
                     KNNTLVVCGNDDFLGALPNAEYKIVKTGVGENFKIGAKNIALEELSSKFTVYDGEKEE
                     EFSLDMPGEHNISNLMLGIAIAKELGVSFEEMKRGLKNIEATSMRLELIKKDGFSILN
                     DCYNSSPVAVKSAIDVMKNIEGKRRIAVLGTMRELGHKSEEAHEEIGKYSKENGIEKV
                     LCFGDFSKNIKEGYGEGCTVYENKEELIKDLLNIICDGDIILVKASRSLKFEEITKAL
                     LEK"
     gene            15497..16468
                     /locus_tag="K8M77_000225"
     CDS             15497..16468
                     /locus_tag="K8M77_000225"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBI7084754.1"
                     /translation="MEILKSMIDPKIVMAIVISFIVASILGPIIIPLLHKLKFGQNIR
                     QEGPKSHLKKAGTPTIGGLIFIFATIITMFVMVGNPTDEAMIALYSFVGFGFVGFLDD
                     LLKIIKKKNEGLTSGQKMILLLIISGFLTWYAYKYIGTSINIPFLNGQINLGLFYIPA
                     AMFYFAGVTNAVNLTDGLDGLATSVTVLVTTFLGIISYNLGHISLAIFCVALAGALLA
                     FLRFNAFPARVFMGDTGSLALGGAVAMVALILKMPLILVLIGIIYVIETLSVILQVAS
                     FKLTGKRIFKMAPIHHHFEQLGWSETKIVSVFSIITVVFCFIAFASL"
     gene            16496..17620
                     /gene="spoVE"
                     /locus_tag="K8M77_000226"
     CDS             16496..17620
                     /gene="spoVE"
                     /locus_tag="K8M77_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBI7084755.1"
                     /translation="MKNKKTTKKSNINPLDYGLLYTIVILLAIGVVMVYSASSYFAMV
                     NYNDSTAFLKKQALFAAIGFTAMIFISRCDYHKLKKLTGILFVITPILLVVVYLFPAT
                     KGAQRWIKLGPFSFQPSELAKYAVVIILANIITNKGEKIKEFWKGIVPCFIVGGGFAA
                     LILAQKNLSIAAVTGFVTFIMVFVAGARKRFMFGVITPLILFAGSFFTLFEDYRRRRL
                     LNFINPWKDPAGDGYQLIQSFYALGAGGVTGLGIGQSRQKTLYMPEPHNDFIFAIIGE
                     ELGLIGCTVVILLFVIFVYRGIKIAMNAKDEYGTLLAVGITSIIGLQAIINIAVVTGS
                     MPVTGVPLPFISYGGTALVFNLMAMGILLNISRQRNKKID"
     gene            17776..18522
                     /locus_tag="K8M77_000227"
     CDS             17776..18522
                     /locus_tag="K8M77_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBI7084756.1"
                     /translation="MSTNINEYIKKKKRKKTRKKIILVSITLVVILGLILIKTPYFNV
                     KKVNVNNNNVITKESVIGENDILNQNIFLLNTSALKKKILSNPYVKSVKISRKLPDQL
                     IINIVERNATFMVNDGADFYVLNKNLVIMEKKNSAEGLQLPTVTGLTVENRFLGEPMS
                     TDKDKVEVLKEIGEALNKSKIKVSSVDISNINNIIINKGEVKILLGNSDKLSDKLTKM
                     VNILNDPAGNFEKGYINISFNGNPVIYKEK"
     gene            18536..19252
                     /locus_tag="K8M77_000228"
     CDS             18536..19252
                     /locus_tag="K8M77_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBI7084757.1"
                     /translation="MKKLSSQIIIGIVCALLGFLLTYQFKIINNKEKNIIESYNQNDI
                     MAEIDGLKKEKEELQKNNDSLNKRLKEMEEAAASKGDVNNKIKEQLDKTRMILGTEEV
                     KGTGIILSITPKSPLLTSKDPEYISEKEIIHILNLLAFSGAEAVAVNDIRITPQTGIK
                     NASNFIWIGNNERISPKEKLIIKVIGNQENLIKGIDFPGEMDYGVLGGNYDYKYEKKD
                     SIIIPKTTQNLSSEYLKKVD"
     gene            19265..19603
                     /locus_tag="K8M77_000229"
     CDS             19265..19603
                     /locus_tag="K8M77_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HBI7084758.1"
                     /translation="MIVFIGLLVGIIIGVIFPGSIPDKFSPYLSIAIVACLDSVFGAI
                     KASLKKEFQADVFISGFFGNAALAALLAYLGDKLGIPLYFAAVIVFGSRIFNNFAAIR
                     RILLDKSKER"
     gene            19616..20347
                     /locus_tag="K8M77_000230"
     CDS             19616..20347
                     /locus_tag="K8M77_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBI7084759.1"
                     /translation="MKNREFTIFIFTASVIVGLLISMNISFDGIETTKVLNAKEYQDA
                     YNKRNNLYNEISKLNESNRELQKKINNYSKNDEDSNKISKDMIEELNNNLMLSGLEEA
                     KGPGLKIVLEDGGEAFSGEVVDDFMRLLRTIHDDDMIKVINELKAAGAEAISINNQRI
                     IYNTEIYCGGQFLRMNGVKIPAPFYVNVIGDPEKMENQIMRDGSYIKTLINRGIKVKI
                     TKSEDITIPAYISDVNSKYMEPNTK"
     gene            20446..21105
                     /locus_tag="K8M77_000231"
     CDS             20446..21105
                     /locus_tag="K8M77_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBI7084760.1"
                     /translation="MDIKENLTKFTKKIPSNVQLIAVSKTRTIEEMEEAYEFGIRKFG
                     ENKVQEIIRKFDEFHKDVEWHLIGHLQTNKVKYIVDKVHLIQSLDSIKLLKEIEKVYG
                     RHNKIANTLIQVNIGREEQKYGILEEKLDELIEAIEACNNVNVLGIMTIIPKGTEEEC
                     RKYFSKTHKLFCELKEKKFKNIKMDILSMGMSGDYEIAIEEGSNMVRIGQGIFGKRLY
                     K"
     gene            21124..21573
                     /gene="sepF"
                     /locus_tag="K8M77_000232"
     CDS             21124..21573
                     /gene="sepF"
                     /locus_tag="K8M77_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBI7084761.1"
                     /translation="MSKVVSKMKSFLGFDEFEDEDEVMEEEEVMEEEESFAPVLSSKK
                     NGKVVNIHTANTAKLMITKPLVYDDATEICTALKNRKIVVINTTSLELRTAQRLIDFV
                     GGACYALCGELQEVEKGVFIVSPSNVEVSNELKSELSNKGMFNWASK"
     gene            21575..22336
                     /locus_tag="K8M77_000233"
     CDS             21575..22336
                     /locus_tag="K8M77_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBI7084762.1"
                     /translation="MNKKDFLNLLNLEEFEALKLFERFNLARTKNISVFTDEFYTPDV
                     WSTLQEMNLFDVNVESFGFFEDGERRVIAFNKEEYDLFPISLLRIQCNTKFSKLEHKD
                     YLGSIMALGINRNKIGDLILRDDGCYVAVHHSIIDFLINNLNKIRNLNCKCEIIYDLD
                     ETPKPSYEEKNIIVTSKRLDCIVAALGNISRSKALDAINSGSVLLDYRITKDKSKEVS
                     EDSRLTIRGIGKFKISNIFGTTKSGRLKLTVFKYT"
     gene            22349..22975
                     /locus_tag="K8M77_000234"
     CDS             22349..22975
                     /locus_tag="K8M77_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBI7084763.1"
                     /translation="MKLTPMDINNKEFKRVLRGYCPEEVDEFLDQIVEEYEILFKENT
                     SLKEKLENSNDKIEHYSKIENTIQNTLLLAQNTAEQAKANAQKEADVIIKSANDTAQR
                     ILDKAHNDVIQINDEYDRVKQEFTKFRAKFRSFMNFQIDTFDDLEKDFIKNFNITKPE
                     DQTETEEPQGIQIDFEEEKEEKTELKDIYEVETHFDDVNEIKSFFAEE"
     gene            23113..24033
                     /locus_tag="K8M77_000235"
     CDS             23113..24033
                     /locus_tag="K8M77_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBI7084764.1"
                     /translation="MSLSKEVIKITEEEKGIRADKFLALKIDGVSRNFIQGLFDDGKI
                     LINGKAEKGKYKLSVGDCVEVEIPEPEELKVEAEDIPLNIIYEDEDVIVVNKPKGMVV
                     HPAPGNYTGTLVNALLYHCDDLSGINGVIRPGIVHRIDKDTSGILVVAKNDNAHQKLA
                     EQFKDHSIKREYYAIVEGRFKKEGGTIDAPLGRHHRDRLKYAVVKDGKRAVTHYEVLK
                     VFNSCSLVKCTLETGRTHQIRVHMAFIGHPLLGDTVYGLKKQKKLSQEGQVLHAKTLG
                     FIHPTTGKYVEFDSELPEYFEKIVEKLGGC"
     gene            24037..24573
                     /gene="pyrR"
                     /locus_tag="K8M77_000236"
     CDS             24037..24573
                     /gene="pyrR"
                     /locus_tag="K8M77_000236"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBI7084765.1"
                     /translation="MHLKASLLDENAIRRALTRLSHEIIEKNKGVEDIVLVGIKRRGY
                     PLAERLSEFIEKFEGVKIPVASVDITLYRDDLTNVSDTPNLNDPKIDVDIRGKKVIIV
                     DDVLYTCRTARAAIDAIMDQGRPEFIQLAVLVDRGHKELPIRADYVGKNIPTSKDEII
                     KVQIKEIDGTDSVEIYEN"
     gene            24593..25720
                     /locus_tag="K8M77_000237"
     CDS             24593..25720
                     /locus_tag="K8M77_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBI7084766.1"
                     /translation="MDYNLIATATFGLEAVVAKELKELGYEDLKTENGRVHFEGDEMD
                     IAITNLWLRTADRVLIKVAEFKAESFEELFNKTVEIDWSKYIPVDGKMHVVGKSVKSK
                     LFSVPDCQSIVKKAVVKSMSRSYGQDWFTEDGPVYKIEVGLLKDVVTLTIDTSGEGLH
                     KRGYREHSGQAPLKETLAAAMVLLSKWRGEQTLIDPCCGSGTILIEAAMIAKNIAPGL
                     HRKFVSETWPSMDKEIWDQVREGAEKSIKKIPLDITGYDIDSWVLSTAKNNVRKAGLT
                     DCITIEKRNFFDFSTKKKYGYMITNPPYGERIGEKEIVSKLNKHFGEVKEKLDTWDFN
                     ILTACPDFQKEFGRKATKNRKLYNGRLLCYYYQYLDNNLKK"
     gene            complement(25831..27558)
                     /locus_tag="K8M77_000238"
     CDS             complement(25831..27558)
                     /locus_tag="K8M77_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBI7084767.1"
                     /translation="MALDGIYLYNLINELKDSLINSRIDKINQPEKDEIIINVRGKEN
                     KKLLISSSSKYPRLHFTTISKNNPLQPPVFCMVLRKYLTGGRIIDIYQQSTDRIVSID
                     IANKDEMGFHSVYTLVVEIMARHSNISLVRKRDNKIMESIKHITANKNSFRVLYPGVS
                     YVFPPASEKLNPFDFSKEDLKIELSKNNNELDEKIFSKLLTGVGKNLSLEMYSLFKSQ
                     FGDSYVFDDIFNFICNYFNNIFKDIQNIIFYKNEKIIDFYFKDLSILDNCTKEVYDNS
                     SELLDAFFANKDKQDRLHAKSADIQKLVNTNIDRCLKKIKVLEKTLEECSKKEEFKIK
                     GELLTSYIYSIKKGDKSVDLLNYYSEDEEYLTISLDENKTPSENIQFYFKKYNKLKKA
                     EESALEQLAINEDELKYLNSVSSSIQVADNYEDIDAIKNELIETGYIRFRRNNNGKKK
                     EKQSKPYHYVSSDGIDIYVGKNNIQNDYLTLKFADKNDTWLHTKDIPGSHVIVKSSNI
                     PDKTLEEAANLAVFYSKGKGGTKIPVDYTLVKNVKKPSGSKPGMVIYSTNKTVYMDSP
                     KEITLEKLK"
     gene            27650..28468
                     /locus_tag="K8M77_000239"
     CDS             27650..28468
                     /locus_tag="K8M77_000239"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBI7084768.1"
                     /translation="MKFSKMHGNGNDFIVIEDLNNEYLGKEGEIAQKMCHRRFGIGAD
                     GILIVRKNENCDIEMVIINSDGSYAAMCGNGIRCFAKYVYEKGIVKKDVLDVLTGDGV
                     KRIFLEIENDKVKTINVNMGFGDFKPKNIPALCDEEIIEKKVSVGNGNLEITSLLMGV
                     PHTIIFEEEKYPIECGRDIEKYELFPQGTNVNFCKVIDRNTMEVRTWERGAGPTLACG
                     TGNCASVIAANKLGFVDKEVKVIVPGGELKVNIEDDGVKMIGNASFICDGTYLF"
     gene            28485..29516
                     /locus_tag="K8M77_000240"
     CDS             28485..29516
                     /locus_tag="K8M77_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HBI7084769.1"
                     /translation="MRKAAALSLLVLVILSTTLAGCNFSKGSNEEDSKGKKLKLPCSI
                     VSTILGEDVLYYEVEGDSLKKLNLDLKGHMEVYSEELGLAVYREFADKGTNLIITNGV
                     KKHKISVEGNIEELIISPKGTKLLYRYNSGDKIGYKVLDLQNFKEFDFNENLAISGEN
                     VRFVNEDQLILYGVDLEKKQSGIYIYNIKDGSYTLEKQIVKAFIDYIDLVEDQVVLYT
                     QSSIDGKKNCYIYNLDNKKESVISNEVGEIESLCKIGNVVYFIGTKGEGLRSLYSIDI
                     TNNKLDRLVYDFPKNISEKSKLIVVGDNIYFLGFNDSKEKNALYRYNLKEKSIKLIDS
                     NKGQYIIIK"
     gene            29617..31005
                     /locus_tag="K8M77_000241"
     CDS             29617..31005
                     /locus_tag="K8M77_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBI7084770.1"
                     /translation="MKYTIKDIDMKLMENISKNTALEMNLIALKESSGKIFFYTSKEE
                     SKKREYLSLLFREKEINFVIGNKAEIEILIEDCYGGKSFSKDKFIEEELISLISLGIE
                     KNTSDIHIEPLEEYANIRFRIDGTLVLVKRIKKNLYENLVNKIKLKADMDISDKLRCQ
                     DGKVSFKNYGYDCDLRISSIPSTFGEKLVVRILYKKNELMNIDNLSLIEEQKNKINKL
                     LKLKHGMVVINGPTGSGKSTTLYALLNSIDKEGINISTIEDPVEFTIEGITQTNVNEK
                     VGLTFANGLKHILRQDPDVILIGEIRDEETAKIAIRASITGHKVYSTIHANSGNEVFN
                     RLLDMGVEGYLVKEGVNSIITQRLVRKICEKCKAKIELKNELVEKLKEEELSLKNYYK
                     GTGCEMCNWTGNNGRKAICEIIFPREYKSTEDITWGEELLKACFTLVKNGEISLEEYY
                     LLKECEGLDVWF"
     gene            30992..32011
                     /locus_tag="K8M77_000242"
     CDS             30992..32011
                     /locus_tag="K8M77_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBI7084771.1"
                     /translation="MFGFKDEASYICLNLSTYVKSGMPVGRSLDLIKNTVKGKKYKES
                     LKRIEKKVLNGEALSKAFKEEGNLYPDLMKDMMRIGEESGNLEEVLEKCARNILAEKE
                     IKNKIIKSLRYPVSLLIGLIIMIFGYITYILPQFVDMYELREIEGSKIMTLLNNYINF
                     VEENPNVEVIVFCYFSCALISLYLLFKVIPSEKILEKIEVYRMYYEIRILFLISMILN
                     SGISLNNSLSVFGESLSDVKLRKYIKSINEELIKGTTLSGSIGKINVISHVSKGFLIT
                     GEESGLFSKNIEELLKIREKDLDRALNKTVGKIEPAMFIILGFVISAMMLLVLVPTFE
                     GMKYV"
     gene            32004..32432
                     /locus_tag="K8M77_000243"
     CDS             32004..32432
                     /locus_tag="K8M77_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI7084772.1"
                     /translation="MYRKRKGFTLSEVIIYMAITSLILAIPTSFMIIKDRGYELKVEG
                     EISKIHDFLMEAKQLSKKDNLYGEIIFDILDNKLIYKNALRTTSLKLNEVKVSFNRDN
                     LITINETGMISIAGTITIIDKNNKQRQITIVPGIWKINVK"
     gene            32452..32964
                     /locus_tag="K8M77_000244"
     CDS             32452..32964
                     /locus_tag="K8M77_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBI7084773.1"
                     /translation="MEGKINKKKGYLLVDMVLALALISILFLGIGKSYSTYIKNNYYI
                     KEKIKVVEIINSLAMELKHNISFEDLRSINSGTYKISTLKINDLINSDIKEFLRKNLI
                     LHNKNHNDFGWTINISHFDQYESIINIIYKNEKPYISENKEVKIYKFLEKSSQNLENQ
                     PQKEEEKHIY"
     gene            33099..33395
                     /locus_tag="K8M77_000245"
     CDS             33099..33395
                     /locus_tag="K8M77_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084774.1"
                     /translation="MYLNHRFWDNMVEDIKINKDKNYILVKFWRDGIVAEDKIEKIKN
                     KLYVYYNYTGYGRNYSPALIEDVEEFDLMEKGNLFYIKLKVKNQEERIYCYEKA"
     gene            33382..33792
                     /locus_tag="K8M77_000246"
     CDS             33382..33792
                     /locus_tag="K8M77_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HBI7084775.1"
                     /translation="MKRRKGFVSIEVMLVSLICSMIVTILMDNSFQRRKELDRSFKIT
                     FNNIDKNNSEEQFLKYMLKEKILNIDNINKLDFTLNNMNATYIEDKKAIHIKRKDKIS
                     GVFKDTYYGIDFRNDDIVFSKRRNYEFVNKDLSN"
     gene            33767..34378
                     /locus_tag="K8M77_000247"
     CDS             33767..34378
                     /locus_tag="K8M77_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084776.1"
                     /translation="MLIKTLVINDTNDISKLKELKDKRVKIILLNEKIFIETLRVDKK
                     CNIEEKIEQLIRDRFFNYTPLVHYEVLKYNKSLFLIVYFIGCDERFKSLLYERKKFSL
                     IFPELKNKNILSFNKATFELKNLKISIYIKGKLVLLKSVKDSNIIEVIEECLKSIEKD
                     FGVYLKDFTFKIQKDYLKEEIKEWFKGLKLNEIRGEENLYQKI"
     gene            34338..34778
                     /locus_tag="K8M77_000248"
     CDS             34338..34778
                     /locus_tag="K8M77_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084777.1"
                     /translation="MRLEVRRIYIKKYKNFLPIEYIEDYNRKIKKKLNIYILLLLLFN
                     LFFYGKVKTENNKIKNYLSEIRGVNRERSLDKEFLEDINKLKEIIKDEKILKLNLERK
                     SFEVEVNSIYKNSLINYINKINGRVYDISLNESNNTYRLKGELR"
     gene            34775..35122
                     /locus_tag="K8M77_000249"
     CDS             34775..35122
                     /locus_tag="K8M77_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084778.1"
                     /translation="MKKLKLDVTLATIFIIILLVQGIELFQLRNYEIPVLTNSIGEMD
                     LSTVLKNIENKDCTVLQLEYKEGWIGEVLFTGSLEDLTNYVNLLKNNNINIKNYKIEK
                     SEELKCFLELKVV"
     gene            35293..35850
                     /gene="efp"
                     /locus_tag="K8M77_000250"
     CDS             35293..35850
                     /gene="efp"
                     /locus_tag="K8M77_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI7084779.1"
                     /translation="MISGSDLRKGTTFELDGQVYTVIDFLHVKPGKGAAFVRTKLRNV
                     IMGGVTDRTFNPTDKLQEAIIERKEMQYLYSDGELYYFMDQETFEQIPLNHEKVEDAI
                     KFLKENMNAVIKFYKGEAFSVEAPNFVELLITECEPSVKGNTATTAMKTAVVETGATV
                     MVPMFVEEGNTIRIDTRTGEYMERV"
     gene            35965..36327
                     /locus_tag="K8M77_000251"
     CDS             35965..36327
                     /locus_tag="K8M77_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084780.1"
                     /translation="MKDIKNNISNLLKAMDKNTELDKEFKDSLLNVISSLVDKVEELQ
                     VNVETLDENVNLLNDDLSGVQDELFEELTLEELEEYDDEYCEVVCDKCHKPIYIEKDI
                     LNRSESIPCPYCGNEFEV"
     gene            36526..37410
                     /gene="spoIIIAA"
                     /locus_tag="K8M77_000252"
     CDS             36526..37410
                     /gene="spoIIIAA"
                     /locus_tag="K8M77_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBI7084781.1"
                     /translation="MKEIFDVLPDKINSCLKDKNNLNKLQEIRVKIGKPLNIVLDNTE
                     IIFNYVIRKEDVKAIIQKISNYSLYAFEEDIRQGYITIQGGHRVGLAGQCVIEDNSIK
                     TIRNITSLNIRVCREIVGCSNSLMNSLVENNRVNNTLIISPPKCGKTTLLRDITRNIS
                     NGISQIGFKGKRTVVIDERSEIAACYNGIPQMNVGMRTDVYDNCIKSEGMMMAVRGLS
                     PEVIICDEIGTYKDMEGLMMAYNSGVSIIATLHGRNVEELYRRPVFREIVENNIINKV
                     VVLSGKKGIGTIEGIYNV"
     gene            37557..38072
                     /locus_tag="K8M77_000253"
     CDS             37557..38072
                     /locus_tag="K8M77_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein SpoAB"
                     /protein_id="HBI7084782.1"
                     /translation="MKVFFLLIIVLLSSLIGYLYGEGFRNRLSQLRELKRALIDFEND
                     IVYTYTPLPESIESIALKAKSPIKELFNEISFKLKNNEVENVYMAFKESINEHKKEMN
                     LRNKDFEILLDLSKSLGETNVEGQIKIFNLAKEKLDIELEIAEDECNKNTKVYRYLGV
                     AVGAMIAIFLV"
     gene            38148..38345
                     /gene="spoIIIAC"
                     /locus_tag="K8M77_000254"
     CDS             38148..38345
                     /gene="spoIIIAC"
                     /locus_tag="K8M77_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBI7084783.1"
                     /translation="MSDLSLIFQIAGVGIVLVILDKVLDQSGKKEYATLANIVGVVII
                     LTMMIQLISRLFSSVKSMFLF"
     gene            38433..38819
                     /gene="spoIIIAD"
                     /locus_tag="K8M77_000255"
     CDS             38433..38819
                     /gene="spoIIIAD"
                     /locus_tag="K8M77_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBI7084784.1"
                     /translation="MDVVIQVISFVLISLFLYLVLKDNKSTFAVFLLLGAGIVIFLFV
                     MPYVNEIIAFMQDIARESGMDSSYIAIVMKIIAIAYLSTFCSYICNDAGVTLLGKKVE
                     FTGKIMILLLAIPIMANILNSILSIM"
     gene            38830..40002
                     /gene="spoIIIAE"
                     /locus_tag="K8M77_000256"
     CDS             38830..40002
                     /gene="spoIIIAE"
                     /locus_tag="K8M77_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBI7084785.1"
                     /translation="MKKLINIILLSLIFVFCFNLKVLANDNLESSKEKIDNDSRIERF
                     YDYINNLETEEDILGNMSAKEYIMNYLESGEDPITLKKIGASILSYIFRELNIVLKFI
                     ASILVIALLSALLKNLQDAFNIEEGVTQIAFFACYALLIMLLTKSFFISLNLGKEVLT
                     SIIDFMNVIVPVIVMLIATSGGVTSAITIDPIVLGAVSITPRIYTYFLFPLILAYFTL
                     QFVNNLSSEFKIDRMCKFIKQVVMISQGFILTIFVGILTLRGMTADTLDAVAVKTVKF
                     AVDNFVPIVGKAFSDAITTVAGYSLAMKSVITSLGLIVLVVIVIYPIIKIGLMAICFK
                     LTSAVIEPVVDSKISSSVETVGEALFMIMSCIISVSIMFFIMASMMATAGNFIVGG"
     gene            40010..40567
                     /gene="spoIIIAF"
                     /locus_tag="K8M77_000257"
     CDS             40010..40567
                     /gene="spoIIIAF"
                     /locus_tag="K8M77_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBI7084786.1"
                     /translation="MELLKNWISTLCVVIVIVSIAHIILPNSSIKKHVKFAFSLIILS
                     VMLSPIIGLLTMNKDIDETVFQEKINDVTKSNEEKKSLYDEEAILKSVEKNLEKSLKD
                     EFYENEFEVNLIGKINFDEVKFNIEKAEITVLDEKKVKKVDKVVVGKEKVNKEEKKDS
                     FLEKVEKFVEKELEISHENIIVSYA"
     gene            40582..41187
                     /gene="spoIIIAG"
                     /locus_tag="K8M77_000258"
     CDS             40582..41187
                     /gene="spoIIIAG"
                     /locus_tag="K8M77_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBI7084787.1"
                     /translation="MHELKEKIANLFKQKNITNLIILLLLVIMFYLVVSYFTGVNNIT
                     KSEKTNLEKVSKEDMNSNSQKDSEVLSYQEKQEKDLERILGKINGVGSVDVVINFQSS
                     EVKVPAVDNSSQKSTTEETDSEGGTRVNSQETDGDKIVMSNSSNGSEPVILKTEKPEV
                     LGVMVVAEGAEDSKIKYEITKAISSLYNISVDKVNVLAMKK"
     gene            41213..41710
                     /locus_tag="K8M77_000259"
     CDS             41213..41710
                     /locus_tag="K8M77_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBI7084788.1"
                     /translation="MNRKQAGIILTLLALIVCTGVLATRVNNQIKESMGEVPTAFGEN
                     DDNATETSSNYFYESRNLREQKDSKTIDNLKAIVEDKNTSAEQKEEAEKELTEKTMAR
                     DYETRIELSIKSKGYEDVICFIDGDNAKVVVKTNEELTQEKMVEIQDIVMNVSKVYDV
                     DIEKK"
     gene            41795..42184
                     /locus_tag="K8M77_000260"
     CDS             41795..42184
                     /locus_tag="K8M77_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBI7084789.1"
                     /translation="MNEMNRDEANLGIVNISDEVIGVVAGIAASEIDGILEINHNNST
                     GIGHKFSKKSLGKGIKVNVENGEAVIEIGVTVQYGIKIPDVVSQVQENVRRTVEAITG
                     LKVALVNIYVQNIIILKEEDKNEILKK"
     gene            42303..42710
                     /gene="nusB"
                     /locus_tag="K8M77_000261"
     CDS             42303..42710
                     /gene="nusB"
                     /locus_tag="K8M77_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBI7084790.1"
                     /translation="MNRVKSREYLLQLAYQMEITSETALETFNSFMENEDISKDDLDL
                     AYIKSGLLGIEENKEKLDSLIESQLVKWKLNRISKVNLSILRISTYEILFAEDVPGKV
                     SINEAIELCKKYSDNKSVSFINGVLDKVYKNIK"
     gene            42759..43592
                     /locus_tag="K8M77_000262"
     CDS             42759..43592
                     /locus_tag="K8M77_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBI7084791.1"
                     /translation="MDKILSGKTVAIDIKGQIKNYTEELKASGKSLKISSILVGDDGG
                     SVYYQNFQEKLANNLGIDFEKIKLDESISEENLKLKIEELNKDDSVNGIMLLLPLPKH
                     IDERVVTNLIDADKDLDCLSEVSVGRFYKGEKCFMPCTPNSVITLLKAYNIEIEGKEV
                     VIIGRSNIVGKPLFQMFLNENATVTVCHSKTKNLKEVCKRADILVVAIGRANFIDSSY
                     VREGAVVIDVGTSEVNGKITGDVNFDDVYEKASLITPVPGGVGSLTTTLLLKNVCKEL
                     D"
     gene            43594..44796
                     /locus_tag="K8M77_000263"
     CDS             43594..44796
                     /locus_tag="K8M77_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBI7084792.1"
                     /translation="MKLKTLSVGEVNNYVKKLVENDFILKNLNVKGEISNLKFHSSGH
                     IYFSLKDENSKVNCIMFKNNAVNLDFRLEEGMKVEIKARLGVYHKEGTYQLYCENIKK
                     AGIGELFEEFHKLKKELSEEGIFDEKYKRALPKFPKRIGIITAKTGAAVRDIINVIQR
                     RNKSLDIILYPAKVQGENAADSIIEGIRYFNNEKSVDVIILGRGGGFIEELWAFNNRD
                     LAYEIFNSRIPTVSAVGHEVDFTISDFVSDMRAPTPSAAGELVSPSLQEMINDLLNKK
                     EFLHRAIDRKFLNAKRDVDLLYKGLKGNNPKDIIEKRIKEVNSLEEKLNFLGKRKIDK
                     AKDELIALNSILQTLNPLNTLGRGYSVIMDKEDKVINNVSELKKNDMVKVIMKDGSVN
                     IDIKIINE"
     gene            44815..45042
                     /locus_tag="K8M77_000264"
     CDS             44815..45042
                     /locus_tag="K8M77_000264"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBI7084793.1"
                     /translation="MARKETNYESMVSELNEIVKQLENGDLTLEESIKSYENGVKIVN
                     KLYKKLSTLEGKIKVVEDEKEEDFGGYSNEY"
     gene            45032..45904
                     /locus_tag="K8M77_000265"
     CDS             45032..45904
                     /locus_tag="K8M77_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBI7084794.1"
                     /translation="MNINSLKEEVDQSLKAYFDKDREYNKVLYDSMAYSVNVGGKRIR
                     PILMLLSYYIYKSDYKKILTPAIAIEMIHTYSLIHDDLPCMDNDDLRRGKPTNHKVFG
                     EAIAVLAGDALLNEAMKILVDYSLDEGISALKATKIIADAAGSDGMIGGQIVDIINED
                     KEEISLKELDYMHLKKTGELIKASIMSGAILAEASERDIKKLEDFGYKLGLAFQIKDD
                     ILDVVGNAKDLGKNVHKDQESNKNNYITIFGLEECKKKCVNITEECIDILSSIEGNIE
                     PLKVLTMKLLERKF"
     gene            45925..47784
                     /locus_tag="K8M77_000266"
     CDS             45925..47784
                     /locus_tag="K8M77_000266"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBI7084795.1"
                     /translation="MSEILQKITDPKEIKDLDEKELEMLAKDLREFLIESVSNTGGHF
                     ASNLGVIDLTVALFKNFDFSENRIIWDVGHQSYAYKILTGRKDKFNTLRQYGGLCGFP
                     KRTESEYDFFATGHSSTSLSSAAGMARAQKILGKDNKVIAVIGDGALTGGMALEALND
                     IGYRKDNLIIILNDNQMSICKNVGGLATYLNKLRMGVGYNKLKSDIGSTLDTTSFGKR
                     VKNSLSKLKDGIKKIVVPSMYFEDIGLKYFGIVDGHNIRELNEVLSIAKSIKGPVIIH
                     TVTKKGKGYELAEKNPNKYHGVSPFDLGEGVISKFANRNYSSTFGEEMIKLAKNDDKV
                     VAITAAMPDGTGLKDFREEFPDRFFDVGIAEQHAVTLAAGMAAEGLKPFFAVYSTFLQ
                     RAYDQVLHDVCIQNLPVTLCLDRAGLVGEDGETHQGIFDISFLSPMPNMTIVAPKCID
                     EMEVILKWASNFNAPLAIRYPRGGDINVNLKPLSKIEYGKWEKVQEGEKIAIVATGKM
                     VQHAMIATQKIKEEKNIDILIINATFIKPIDKELLNSLSKDGFKIVTIEDNIKKGGFG
                     EGVLEYLNEIGHEEKIVTLAFNDKFIEHGKPDILYKINGLDAEGIKNTLIELL"
     gene            47796..48611
                     /locus_tag="K8M77_000267"
     CDS             47796..48611
                     /locus_tag="K8M77_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBI7084796.1"
                     /translation="MAEKKEKKERLDVLLVEQGIAESREKAKRYIMAGMVFIGEKRVD
                     KAGEKVPVSSNIIFRGEKLPFVSRGGFKLDKAVKSFGIDLKGKRCLDIGASTGGFTDC
                     MLQNDASKVFSIDVGYGQFAWKLRVDPRVVCMERTNVRYVTPEQIGELCDFASIDVSF
                     ISLTTVLPAVLNLLNDKGEVMALIKPQFEAGREKVGKKGVVREASTHKEVIKKIVDFA
                     LSHKLNILGLDFSPIKGPEGNIEYLIYLKKANNTEEFDYNIIDEIVDKSHSDL"
     gene            48695..49525
                     /locus_tag="K8M77_000268"
     CDS             48695..49525
                     /locus_tag="K8M77_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBI7084797.1"
                     /translation="MRNIGIIINKEKDKENKILNLVILKVKEYLNPDEIKVIDQFYKG
                     DYKDLMSLDLLIVLGGDGTLLGVARKFSTVIDTPILGINIGNLGFLVTAEISELDEAL
                     YRIKVGDYKVEERMLLSCTIEGVTCSDERALNDIVVARGTLSRMAQYEVFINDELYAT
                     FKGDGVIISTPVGSTAYSFSAGGPLIMPDLQIVSIVPICPHTPNSRPMIIDGNNKVRV
                     KPLINESDVFVTIDGQKALKLEKHNEVLIKKAKEFFRIISFDNKSYFKVLRKKLFKIE
                     "
     gene            49548..50000
                     /locus_tag="K8M77_000269"
     CDS             49548..50000
                     /locus_tag="K8M77_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBI7084798.1"
                     /translation="MKSKRHSKIIEIIKSTAIETQEELAEELKKAGFDVTQATVSRDI
                     KNLKLVKKQDNKGHYRYTIIEEEKNEMVDRLSNILENTVTSVENVEKMVVVKTISGSA
                     SAAAEAIDTLNLGEIAGTIAGDNTIFILVRSLEKAETLVSRILEMIEG"
     gene            50016..51713
                     /gene="recN"
                     /locus_tag="K8M77_000270"
     CDS             50016..51713
                     /gene="recN"
                     /locus_tag="K8M77_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBI7084799.1"
                     /translation="MLLQLTINNFALIEKASLDFKEGFTILSGETGAGKSILIDAINY
                     VQGSKFNKDLIRTGEEKTFVEAIFSIDDNERLKEILDDLEIEYDDTLIISRETFINGR
                     SNIKVNGRSIIVATLKKISSTLLDIHGQHNNQNLLNKENHIMYLDNFGDSKLSEDLKE
                     YGEKFLELKDIEKKINELSNEGKDEKLLNYLEYQLNEIEEAKLRNGEEEELTERFNVL
                     SNAEKIKNSLGISYNLLNGIESSVVDSLSVVNRELSNVEEHLEKIKNINSKIMEMYYE
                     IQEMAREIRDICDESVYDGNELEEINSRMFKIASLKKKYGNSIAEILEYKNNILSQIN
                     NIKNSEKIIQDLLNEKSKVENVLSEIANRIHNKRIELSKVLEENIHKELAYVGLGKCR
                     FEVLIEEDETFNFKGKDKVQFLISTNPGEPLKPMERIVSGGELSRIMLALKAVFIDKD
                     KIPTVIFDEIDTGISGRVAQSVGEKMYEISTKHQVFCITHLPQIASMSDNHYMVRKKV
                     IDNKTFSKVEPITYNQKIEEVGKMLGGVEMTSNTLLNAKEMIELADTKKETIKTFHT"
     gene            51890..53050
                     /gene="spoIVB"
                     /locus_tag="K8M77_000271"
     CDS             51890..53050
                     /gene="spoIVB"
                     /locus_tag="K8M77_000271"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HBI7084800.1"
                     /translation="MKNMNKRIKIMSIIMMSLILLLSSVTFARDYCESNNVFASSNFY
                     SLNYKSGNDKKFKNRYGVALVNSELEKKDIELYAGGNSVGVRVSTDGVLAVGYSSLAM
                     GEGEIESPAQNGGIQIGDRLISVNGDKIKNSKDLSKKINESKSENVEILIERNGEKIT
                     KNINLSKNADGDYKIGLWVRDSTAGVGTLTFYDKESGKYGAIGHPITDSETEKILSIK
                     NGDLLNSSIISIKKGVKGNPGELRGIFSSDKTPIGNVTGNTQCGIFGSMNKENFKNIN
                     NKTYKVGWRDEIQPGPAQIITTIDEDGPKLYDIEIVKLAKQDSISTKSMVIKITDERL
                     LEKTGGVVQGMSGSPIIQNDKIIGAVTHVLVNKPEIGYGIYIEWMLKDAKII"
     gene            53224..54054
                     /gene="spo0A"
                     /locus_tag="K8M77_000272"
     CDS             53224..54054
                     /gene="spo0A"
                     /locus_tag="K8M77_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBI7084801.1"
                     /translation="MKESKISVLIADDNKEFCNILNDYLLSQRDIVVTGIAKDGLEAL
                     KLIEEKQPDLVVLDIIMPHLDGLGVLERINSLGLEKTPRVIVLSAVGQDKITQRAITL
                     GADYYVVKPFDMDVFTERIRQMFNSTISEEPTNSKPKISVVEGSKSEDSFSEDNKEPM
                     DLEVEITSIIHEIGVPAHIKGYMYLREAITMVVNDMELLSAITKELYPSIAKKYNTTA
                     SRVERAIRHAIEVAWGRGQIEAINKIFGHTIHNDKGKPTNSEFIAMVADKLRLKNKVS
                     "
     gene            54132..54437
                     /locus_tag="K8M77_000273"
     CDS             54132..54437
                     /locus_tag="K8M77_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084802.1"
                     /translation="MRKAVYVEGNEKKIILANCITKSEYEKIYKKNLVCPIDGCKAKL
                     GFREVKKQRDGKYFYTLPNNKHVYGCPYKIQKKKRRARRKIDNNLSEDFSQISFEIL"
     gene            54498..55028
                     /locus_tag="K8M77_000274"
     CDS             54498..55028
                     /locus_tag="K8M77_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBI7084803.1"
                     /translation="MDLYEKTLSEESIFKCSFMELVKQKVKLPDGNEAERDIVKHSKG
                     VCVIAFNEKGNILMVEQFRKPFNRVFLELPAGKVDKEEILEKAALRELKEETGYLANK
                     ITYLGQIAPSPGFCDEVVYLYKAHELKKGNTNFDHDEFLNLKEYPLEEVKKMIIEGKI
                     TDAKTIACLFFYENIK"
     gene            55413..56033
                     /gene="spoIIM"
                     /locus_tag="K8M77_000275"
     CDS             55413..56033
                     /gene="spoIIM"
                     /locus_tag="K8M77_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBI7084804.1"
                     /translation="MRMFFRGLMESLKENKITFLWVLLFFLVGIVLGSYTVYYMSEFN
                     RGEITTYFNNFLEFLGGNSVSYTSILVDSIKSILPIVVIIVLLGYTAVGTPIILMIDL
                     AKGYVIGFTFSLIVSMLGSKGVILVLSGLMIQNLIFIPIIMLISVLAIRYSFTKLKIK
                     ASRERINFDVSRKYLNFQGILSLIMISGILIETYISPNLIRLVISR"
     gene            56083..56955
                     /locus_tag="K8M77_000276"
     CDS             56083..56955
                     /locus_tag="K8M77_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerD"
                     /protein_id="HBI7084805.1"
                     /translation="MKDLVSKYEVYLESIGKRKNTVSAYITDVSMYLDFVYEKGLKVS
                     EDSYPLISYVQHLKESHKSPSSIQRTIISLRNFYNFLVAEEFLKKVPNLTMKKERIEK
                     KKPLVLTVEEINKIMNSTNVLTEKGIRDKALLELMYATGMKVSELISLRIEDINLDSS
                     FVNCRDSRGYERLIPIGKEAKESIKRYIVVREDNSKGSDKLFLNMSGEPITRQGVWRI
                     VKEYAVIANIGKDINLNTFRHSFAVHLLQNGANAKVVQELLGNQVMTYIDMYYDIINN
                     EKINNIYRKAHPRA"
     gene            57183..58484
                     /locus_tag="K8M77_000277"
     CDS             57183..58484
                     /locus_tag="K8M77_000277"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBI7084806.1"
                     /translation="MRMYDLILKKRNGGELSTEEINFFVDKYTNGEIPDYQVAALLMA
                     IYFQKMNKRETSDLTMAMVNSGDILDLSEIHGIKVDKHSTGGVGDTTTLVLGPMVSAL
                     GIPVAKMSGRGLGHTGGTIDKLESFDGFSVEMTKDQFINNVNNIKLAVGGQTGDLAPA
                     DKKLYALRDVTGTVDNVSLIASSIMSKKIAAGADAIVLDVKVGDGAFMKTPEAARELA
                     TEMVGIGKHVGRNTVAIISDMDQPLGFAIGNALEVKEAIETLRGNGPKDLLELCLTLG
                     SNMVVLAGAAKDTDEARKMLMETITSGKAIEKLKEFVKAQGGDASVIDNISNFHNAKY
                     VIPVKANKSGVVSKIHAENIGLVAMELGAGRATKESIIDLAVGIVLQKKRGDKVNDGD
                     IIAYIHANDEEKGNKAIDGILANYEISDSVKDIPLIYDIVK"
     gene            58736..59944
                     /locus_tag="K8M77_000278"
     CDS             58736..59944
                     /locus_tag="K8M77_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBI7084807.1"
                     /translation="MKKYIRKIAAFSIFLSLFSIVSSVPVKAEEAIEVEARSALLMEA
                     STGKIIYEKNANEKFAPASVTKVMTMLLAMEKVDRGEIKLSDQITVSETAKKMGGSTM
                     LLDVGEVRTVEEIIKGIGIASGNDAAVAMAEYLGGSESGFVEMMNKRAEELGMKNTTF
                     KNCTGLPIEGHLSTAYDISIMSRELLKHPTILKYTGTYMETISEGRKSPIELVNHNKL
                     VRFFKGCDGLKTGFTNDAKYCISATATRDGVRMLSVIMGAPTFKIRNRDASMLMNYGF
                     SKFESKQVATKDGEIEQIPMNQTGDKFIIGKAKDDLTLVCPKGMSDKIEKKVVLDESK
                     KKIKEFDTIGYCEYYLDGELLGKVELYSDRDMKKGGIIDSVKFKVKNFFNGEEEDKVE
                     EKTEEKSNEK"
     gene            60223..60969
                     /locus_tag="K8M77_000279"
     CDS             60223..60969
                     /locus_tag="K8M77_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBI7084808.1"
                     /translation="MEMPIIKLKNFDGPFDLLLHLIKKNEMSITEIKIHEITKQYLEY
                     IALMKELDLEITSEFIVMAATLIEIKSKSLLPKVKVEDETCEEDLQKILMEKLQEYKK
                     FKKISAYLRERELSTGEVFTKKAEIIEVEVDNKLDDDYFKNITMLDLYKLYNNLMRIY
                     GEKQNVNVMEKKISVDKYKITDKINFLRDKLSEKSVVRFSEFIPQCECKLEVVVTFMA
                     MLELIKRSEIKVVQYENFGEIMMEKVIVNE"
     gene            60962..61549
                     /locus_tag="K8M77_000280"
     CDS             60962..61549
                     /locus_tag="K8M77_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HBI7084809.1"
                     /translation="MSDINQIEFSEISKKDELKSIIESLLFVSGEPLALKDICRIVEE
                     DFKYVEDLMRELMNIYNGDSSRGIKIISLNGTYQLVTKTKNSEYIQKLLKKNVRQSLS
                     QASLESLAIICYKQPITRVEIDEIRGVKSESAIQRLVEKNLVEETGRLEVPGRPILYG
                     TTDEFLRHFALNDLGDLPSIELFEENNEVSDMVEE"
     gene            complement(61878..62297)
                     /gene="ytfJ"
                     /locus_tag="K8M77_000281"
     CDS             complement(61878..62297)
                     /gene="ytfJ"
                     /locus_tag="K8M77_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBI7084810.1"
                     /translation="MINSNHPIENLMGTTMENIRDMIDVNTVVGDAIETRDGSYIIPI
                     SKVSFGFASGGSELPNKECEKIDDRFAFGGGSGAGVSVKPVAFLVLKGDSVRLLPVNQ
                     TNTYDRIVDNIPQIIEIFKDMGNKKCKDKKEDSNFCD"
     gene            complement(62344..>62741)
                     /locus_tag="K8M77_000282"
     CDS             complement(62344..>62741)
                     /locus_tag="K8M77_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBI7084811.1"
                     /translation="SKKGNNDTKIKKSSKKKRTFLKPPSNLTIMKLIKLLTKSKFKPS
                     LKIVLDLNFSSEDSALTAILYGFIHQILSLIYTGLNYFFKLKKFTGNINPKFNNENYL
                     FFEVQGIITINFAQIIYIGFLYLLNFKKL"
BASE COUNT        25268 a         5994 c        11532 g        19947 t
ORIGIN      
        1 acatttttac ttttttttgt tttcaaattt taaaatattg tagtataata tctgtgaaaa
       61 taaaatataa aatctttata gagatttaaa ggggagatag tgcagtgtat agagaaaaat
      121 atgaagaatg gctaaattct gatattattt ctgaggaaat aaaggcggaa ttaagagacg
      181 taaaagaaga taaggaaata gaagatagat tttataagga attagatttt ggtactggtg
      241 gattaagagg tgttattggt gctgggacta atagaatgaa tatctatact gtaggaaaag
      301 ctactcaagg atttgcagat tatttaaatg ataattatgc gggggaaaaa tctgttgcaa
      361 tagcttatga ctcaagaaat atgtctaagg agtttgcaaa agcggctgct ttaactcttt
      421 gtgcaaatgg aataaaggtt aatctttttg agtctttaag acctactcca atgttatcct
      481 ttgcagtaag agaattaaat tgtaagggtg gtattgttat aacagcatca cataatccaa
      541 aacaatataa tggatataaa gtttatggag atgatggatg tcaattaaca gatgcgcctg
      601 caaaagctgt tattggatat gttaacaaag ttacagatta cgcaaacata aagacaatga
      661 gtgaagaaaa ggctttagaa gaaggacttt tagtttatat tggtgaagaa atagataaaa
      721 aatatataga tgatttaaaa actttaacta taagagaaga tttagttaag aaacatgcta
      781 aagatttaaa aataatttat actccaattc atggttcagg aaatgttcct gtaagaagaa
      841 tattaaaaga gttaggatat gagaatgtat ttgttgttaa agaacaagaa atgccagatg
      901 gaaatttccc aacagctcca tatccaaatc cagaagatcc aaaggtattt aaattagctt
      961 tagacatggc taaagaaatt caacctgata taatatttgg aacagatcca gactgtgata
     1021 gaataggtgt tgttgttaaa gacaataatg gagagtacca agtattaagt ggtaaccaaa
     1081 caggaatgtt actaactaac tatatattaa gttcattaaa ggaaatgaac aaattaccta
     1141 aaaatggtgt agttataaaa actatagtta ctacagagtc agtaagaaaa atgactgagg
     1201 aatatggggt aactttaata gatacattaa caggatttaa atatataggt gaaaagataa
     1261 gagaatttga agaatcaggt tcaaatgaat atttatttgg ttttgaagaa agctatggtt
     1321 acttagccgg aacttttgct agagataagg atgctgttgt agcttcaatg ttaatagctg
     1381 aaatgacttt atattataaa gaacaaggaa aaactcttta tgatggttta atagaacttt
     1441 ataataaata tggttacttc aaagaaagct tagtatcaat agagcttgca ggaaaagaag
     1501 gacaagaaca aatagctaag tgtatagatg gcttaagaaa tgatgcttta aaagaaatga
     1561 atggagttaa agttattaca tctttcgatt ataagttaag caaagaagta aataacttaa
     1621 ctggagaaga aaaagaagta aaacttccaa aatcaaatgt tttaaaatat gttttagaag
     1681 atgattcttg gtttgttgta agaccatcag gtacagagcc taagatgaaa atctacttat
     1741 cagtaaaagg ttcaagctta gaagattcaa aagaaaaaac tgaaaacttt aaaaatgcaa
     1801 taatggaagt aattaatgct aaattaaaat agtttaagtt aaacaaagag ctatgttaat
     1861 ataacttata taaaatttat aagttataaa tatagctctt tttatatttt tagagcttaa
     1921 tgcacaaaag agttaattaa agtgttataa tatataacta aaataagtgt tataagttat
     1981 aactaaatac caagatgaac taatttttat tttgtgataa aataaagttc gaaatttatg
     2041 atatataata aaatttaggt ttataaattt tatttttaga tagaattaaa atagttagta
     2101 aaagtagtta aggagataga tatatgagtt ttaatacatt tgcaaaagaa aaattatcac
     2161 aacttttatt tttagaaata gatggagatg gatttgttaa aagcttagga aaagatccaa
     2221 aagaggttaa tataaatgaa gtttatatcc caatagatcc aaaacattta tcacaggatg
     2281 tgaaaagtgg atataaatta gaatctttac caataaacta tttagttgaa ggaatgtttt
     2341 tcgctttagg gggagacaag aattttaaat ttaataagga gtataaaaaa ttaattccac
     2401 taatagagga tgctattcca tgtgttaaaa agatagtagc tgataaggtt aaagaagaaa
     2461 atatgattga agcatttatg cttttaaagg gattaagcga aatatcagat gaaactgagg
     2521 tttatgaaaa tcttctttta atatgtgaat ctttaagaga aagaaataaa gcttataatg
     2581 aaactcaact agaaatttgt gataaatgta aagcaaatag aagtgattta gctcttccat
     2641 atctttattc agcaatagct tacaatgaca ttgggcaata tgataaagct tatgttgata
     2701 taaatgaata tttagctaag ggtggagaaa gaaatgaaat agttgaggtt ttatataatg
     2761 agataaaaga ctcagctgat tatgaagaag gaaaagaaga tttaatagaa gaaccagagg
     2821 atgctttaaa aagacttctt cctttagcag ataaattcca agataatgct attttaagat
     2881 attatattgc aacagcttat agaagacttg gaaattttga aaaggcagtt tattacttaa
     2941 atgaatgttt atcaattgat gatagtatag ttgaagcagt aaatgaaatg ggaataaatt
     3001 atgcatcttt aggaatatat gatgaagcta ttaaatattt aagaaaagcc tttgaaagca
     3061 caagagatat agaggtttgt actaacttaa tagtttgtta tttaaatgca ggaaaaatag
     3121 aagaggcaaa gcagcattta gatatagcaa aggctataaa caaggatgat gaaatagtaa
     3181 aagaaataga aacatttatg aaaaataata atattaaata gccttaggag cggaatatgt
     3241 ataaggagtt attaggatat aaaatatttt cagataataa agaggaactt ttaaaagaga
     3301 ttgaaaataa aaaaagagtt aacataatat ctggaaatcc agaggtttta tataatggta
     3361 taaagaatga atttttaaaa aatagcttta ctaaagagga tgctttaata attccagatg
     3421 gagtaggaac tctgcttgca gctaagatta atggaataga tatcacagaa aaaatagctg
     3481 gcattgaagt tatgaatatg cttttagagg aagctagaga taagaattta aaagtgtttt
     3541 tattaggagc taaggaagag atactaatta aatgcaagga aaaaataaaa gaaagctatg
     3601 atggaattga tatagttgga agtaataatg gcttttttga tttagataat tgtgatgatt
     3661 taatagaaaa aattaatgaa agcaaggctg atatactttt tgttgccatg ggagcgccta
     3721 gacaagaagt ctttatagag aaatataagg ataaactttg ttgcaagatt tttatgggtg
     3781 ttggtggaag ttttgatgtg tttgctggaa atgtaaatag agcaccacaa tttatgatta
     3841 atataggtat ggagtggctt tatagagtgg ctaaagagcc ttggagaata aaaagattag
     3901 gtagcatacc aaagttctta gttttatcaa ttaaggaaag aggtaaaaga taatgaaaat
     3961 tttatttata gcttgttatt ctcctatgat aaataattca gcatcaattg aaactcttat
     4021 gtacttaaat aatttatgta atatagagaa taattgtgtg catcttttaa ctgtagactt
     4081 tcctaaaaac tctatatact atgatgagga aatattaaag cttttagata gtaaagtaaa
     4141 ggttcatgct attgaaggtg gaaaattatt taataagatt atgccaaaaa aatctatagg
     4201 ggcaaaggaa gatgagaagt cttctaacac aaaatctagt agtaaaatta aacttatgag
     4261 gaagattaaa aataaaataa tttttcctga tatgtattat aactggagct ttaaagcttc
     4321 aaagtatggc atagaactta tgaataaaga aaagtttgat gttatatttt ctatgcatga
     4381 gccaccatct agtcaccttt gtgctttaag aataaaaaag cactttaaag agattccttg
     4441 ggttttatat tggagcgatc cttggcttaa ggatccatca agagagaata ttggttttat
     4501 aagaaaattc atagaaggta gacaagaaaa atcagtagta ttaaatgggg atagacatat
     4561 atttgtaact gaagagaata aaaaagattt tatggaaaaa tataatgtaa aagaagataa
     4621 aatgtttatc gtaactaggg gatacaataa agccatatat gaagaaattg aaagggcaga
     4681 aaagccagaa cttttaaaga ataataagat aaacttaatt tatgctggag aaattttcag
     4741 taaaattagg gatttaaaac cttttataaa agctttaaaa gaattagaga aaagagatca
     4801 ggagctattt aatagattaa acataatatt ttttggaaac atagatgatg aaaatattaa
     4861 agaagaatta aaaaagtttt ctaacgttag tgttaatgga agaattgact ataaggaagc
     4921 tttaagatat atgatacatg gagatgttct tcttgtttta ggaaacaaaa attctaagca
     4981 aatacctgct aaaatatatg actatttagg aacaaagaat cttattatgg ttatattagg
     5041 agatgaaaat gatcctatta agaatgttgc acttaataaa gaaaagtgta tagttagtga
     5101 aaataattat gaggctataa tagatgactt aaataaatgt agagatttaa tagattcagg
     5161 gaagaaattt aaggcaaatg aagaatatga atggagtagt ataggtaaga ggctaaataa
     5221 tatactaaaa ttaaaatagg caataagaca tgaaggaggg gtagacaatg aaaaacgaaa
     5281 agattaaaga ttttatagaa gatttaggtt caagttcatc agcacctggt ggtggggcag
     5341 cagcagggat tgttggagca gtaggagttg cattaacatc aatggtttat agcttaactg
     5401 ttggtaaaaa agcttacgag gcattaagtg aagaaaataa aaagaaatta gatgaaaatc
     5461 ttgaaaatgc aaaaaaatct tataatgaaa tgttagattt catgaacaaa gatgaggaag
     5521 cttttactgc attaatggat tgctataagt tacctaagga aacttgtgaa gagaaaagct
     5581 taagaagtga aaaaatagat gaatgtactt taggagcaat gatggttccc ttagaattaa
     5641 gcagaaaagc tttaaagttt tttgataatg taaaatttgc ttctaagtat ggaaataaaa
     5701 atttaatttc agatgctgta gtaagtgcta taatgcttag tgcttgtata gatagttcca
     5761 tagttaatgt agaaataaac ttaggatttt taaaagataa aaacttagtt aagagtgtaa
     5821 aagaagaaat tttacatata agaaatgaaa gcaataaatt aaaagaagag attttaaaag
     5881 aaagtggttt tcttcaaaat taagtaaaaa tagaataaaa aattattaga taaataataa
     5941 ggggtctata tcaaaatgta ataaaaaaac ttataaactt atttcgagaa ttaatagatt
     6001 aagctaaatt taaaggttct cttcatacta tttgtaagtt ttataaaaag ttattttgat
     6061 aaagccccct taattaattt agtattattc ttttttagga gttaaatcat ttaaagattt
     6121 aaattcataa ccattagatt ttaactcttt taaaatttca tctaaaatgg cagtattagt
     6181 atctgatact gcatgaagaa gagctacttc accattatga actttatcta atattttttt
     6241 cttagcagct tctttagaag gttgctcatc aactaaccaa tctttataag caaaactcca
     6301 aaaaacagag gtataaccaa gttcttcagt gtaagctaag gattgttcac tgtatttacc
     6361 cattggaggt ctaaagaatt taggcatttc ttttccagta actttagagt attcctcttc
     6421 aacttcagta aattcttttg taaatttttc tttatcaagt attttagcca tagatggatg
     6481 atgagaagtg tgattacata caagatggcc ctcttcatcc attcttttta taaggtctgg
     6541 atagtctttt atatatggtc ttgttacaaa gaatgcagca ggaacttgat tttcttttaa
     6601 tatatcaagt atttttgctg tattaccatt ttcatatcct tcatcaaaag taagaaaaat
     6661 ttcttttttt gatgtgtctc cacaatatac aactttatga ttacttatat aaggagtttc
     6721 ttttggtcca tgaggaatac cttcctcttt attaaaagaa taataccagt taaattcttt
     6781 tgtacaagga ctattaaatt cttttgcact tacttttgtg tttaaagtaa tagatagaat
     6841 catcattatt gaaaataata atgaaaaaac tttcttaatc ataaaatatc acctcaaatt
     6901 tagtatgtgt tatatattag gattaatttg tatttttaag tggataatta ggaaacataa
     6961 tacagttgtt gaaatatata ttaagctaga gtataatcat taagtatgaa attaaatata
     7021 agaaagggac gtgtacatag atgaaattag gaatggttgg attaccaaat gttggtaaaa
     7081 gtacattatt caatgctatt acaaaagcag gagctgaatc agcaaactat cctttctgta
     7141 caatagaacc aaacgtaggt gttgtaagtg taccagataa gagattagat gttttagaaa
     7201 aaatatataa tacaaagaaa aaagtatata ctgcaataga gttttatgat atagcaggat
     7261 tagttaaagg tgctagcaaa ggtgaaggat taggaaataa attcttatca cacataagag
     7321 aagttgcagc tatagttcat gttgtaagat gttttgacga tgagaatgtt gttcacgtag
     7381 aaggttctgt agatccaata agagatatag aaactataaa cttagagctt atctttgcag
     7441 acttagatgt tcttgaaaga agaatggaaa agactatgaa gttagtaaga tcaggtgata
     7501 aaactgctaa gtttgagtat gatgtaatgg aaagattaaa agctcactta gaagcaaata
     7561 aaccagctag aactttagaa gctactgaag atgaagaagc tttcgtaaaa agtttattct
     7621 taataacttc aaaaccagtt ttatatgctt gtaacatatc agaagatgat atgatggaag
     7681 gaaacttaga taatgagtat gttaaaaaag ttagagcata tgctgaaact gagaattcag
     7741 gaatcatggt tgtttgtgct aaacttgaag aagaattatc aggattagat gaagaagaaa
     7801 aagctgaaat gttatctgag tatggattag aagaatcagg tcttgataaa cttatacaag
     7861 caagttataa attattagga ttaatgagtt acttaactgc aggtgtacaa gaagttagag
     7921 cttggacaat aaaacaagga actaaagctc cacaagcagc aggtaaaatt cattctgata
     7981 tagaaagagg gttcataaga gcagaggtag tttcttatga tgatttagta gaatgtggtt
     8041 cagaagcagc tgctaaagaa aaaggtgttt acagattaga aggtaaagaa tacgtaatga
     8101 aagatggaga tatagttaac ttcagattca acgtataata taaaagaaaa aatatcatca
     8161 aatttaaaca gtatttttag acaaaaaaag agctatttga aactttcttc agatagctct
     8221 ttttctttat ggcgggaata tgtgggaatc gaacccaccc atgatggtat tagcaccata
     8281 actaggtttt gaagacctgt agagacacca gcccctatct actcccataa tattttaaaa
     8341 ttttacatca aaataagtat aatgtcaaat ttttttctaa gaatatttta attatataaa
     8401 cttataaatc ctgaatattg aaaggaattt ttaatttttt atagaataat gtataataaa
     8461 atataatata taatgggaaa atagttgcaa tagcattgta ttgtagtgta aattagtaag
     8521 aggtatgata atttacagaa tgtttataat aaatacgttt aaaaatgttt attaataaaa
     8581 ttaagcatga taaattaagg ttattaggct atattataca gtatataata tgattgataa
     8641 aatcaaatga tgataagtaa ttaaaaattt aaggagtatt tatatggaat ttaaacatgt
     8701 ttcggttttg ctagatgagt gcataaatgc tttaaatata aaagaagatg gtatatatgt
     8761 agactgcaca ttaggtggtg caggtcattc aagtgaaata gtaaaaagac tatcaagtga
     8821 tggtagatta ataggattcg atcaagataa agatgctttg aaagctgctg gagaaagatt
     8881 gaaagactat aaaaatgtta catatgttca tagtaatttc tatgctatat atgatgtatt
     8941 aacagattta ggcatagatg gagtagatgg tatccttatg gacttaggag tttcttctta
     9001 tcaattagat aatggagaaa gaggatttag ttatatgcaa gatgctcctt tagatatgag
     9061 aatgaataga gaaaatgaat tctcagcata tgaaatagtt aatacatatt cagaagagga
     9121 attatataga ataatcaaag aatatggaga agaaaaattt gctaaaagga tagcgagttt
     9181 catagttaaa aatagagaag aaaaaaatat agaaactact ttggaattag ttgagataat
     9241 taaagcggca attccagcaa aggctagaag agaaggacca catccagcta aaagaacatt
     9301 ccaagctata agaatagaag ttaataagga acttgaaatt ataagtaaga ctattttaga
     9361 tggagttaag aaacttaata aaggtggaag aatggcaata ataacattcc actcattaga
     9421 agatagaatt gttaaaaata catttaaaga attagctaat ccatgcacat gcccaagtga
     9481 gtttcctgta tgtgtttgta atagaaaacc agaagttaag cttataagca gaaaacccat
     9541 tgaagctagc aaagaagagt tagaatttaa tccaagaagt agaagtgcta agttaagaat
     9601 aatagaaaaa ttataatatg atatatttag aaagttttat tagattttta agtaagggga
     9661 atacaaaata attggagagg gattgtagat gattgtaaaa gagtttgatt ataatggtag
     9721 ttcagcactt aaaccacaaa gaaaagaaca aactatacca agtaaagaaa cctatgacaa
     9781 gaaaaagaaa aaaatcgaac aacaaagaag aaaaaagcaa aaagataaaa aaataagaaa
     9841 aagtgtatat caaatagtac ttctaatatt agttgttggt gggataacaa taagcagaga
     9901 tgtaaaagtt tataaaactc agcaacaatt ggattcatta aataaagaaa taaattctgt
     9961 tataacggaa aatgaagctt tgaaggttca aatattaaag gcaagttctt tagacaaaat
    10021 agaggaaact gctaaaaata aattaaaaat gatattacca actaaggaaa atatgattaa
    10081 gttaggcgaa agccaagcta ctgaaggtaa gcaatagggg gataaattag gatgtacttt
    10141 tattattttt ttaagagtac atccataatt ttttataagg gctacaattt tataaattta
    10201 cttttaatta gataacatga taaatttagt agaaccttaa attttcgtaa agtgaatttt
    10261 aaagatagaa ctcattttat ttaacttact acggagggct aagggtgaaa aataagaaaa
    10321 acaaaggatt taaagattat aaaataacaa aatcacttag atttaaaaga acatattgga
    10381 ccatgatagt agtatgggga ttactaggag tattaacctt aagattatca tacataatga
    10441 tttttaagca taaagaatat ggttcaatgg cagaagaaca atggaaacat gaaataaaaa
    10501 taggagctaa aagaggagaa atactagata gaaatggagc tcaattagct gttagtgcta
    10561 atgtttatag ggtagattta gatctcaaaa cgctaagaga agatacattt actaaagatg
    10621 atacagatga tacaaaaaaa gaaaaactta ataaaattgc aggggagtta ggtactgctt
    10681 taggcatgcc taaagaagag gtatatgata agataaatag tacttttcca tcaggattgc
    10741 cagcgacatc tgttacttta atcagaaaga tagaaaaaga taaagctgat agtgtcaaga
    10801 atttaaagat aagaggagta atagtttcac aagatacaaa gagatattac ccagataata
    10861 atttcttagc acaagtatta ggaagagttg atgcagatgg tataggtcaa ggtggtatag
    10921 agcgtgaata taatgtggag ttatcaggtt taccaggaat gagaatatca gaagttgcaa
    10981 gaaatagtag tgggatacct tattcaaatt ctgagtttgc aaaacctgtt gatggtaaag
    11041 atgtcacact aaccgttgat gaaactatac agtattttgc agaaaaagtt gcagaagaag
    11101 gaaaaaaaga atataaagca gatggggtta gtataatagt tatgaatcct aaaaatggag
    11161 aaatattagc tatggctaat aaaccagatt ataatcctaa tgaaccttat aaagggtatg
    11221 aaaatttccc tggtaaagat aaaactgaaa agatggaaaa tatgtggaaa aatgatgccg
    11281 tatcaaattc atttgagcca ggatctatat ttaaagtggt aacatcttca gctgccgtac
    11341 aagaggggat agcaggtgga aatgaaacat atttttgtcc aggtggaaaa aatgtatctg
    11401 gaacttatat aaaatgttgg aagccagatg ggcatggaac tgaaactttt gatcaaatat
    11461 tagaaaactc ttgtaacgtt gggtttatgg acataggtca aaaacttgga aaagaaaaat
    11521 taaatgaata tatagaaaag tttggatttg gtaagcagac aggaatagat ttacctggag
    11581 aaacaacagg tatagtattg ccaaatgata aaataggtcc agtagaactt gcaaccatat
    11641 catttggtca aacagatagt gctagttcag ttcaaatgat ggcagctatg aacacaattg
    11701 ctaatggagg aacatggata caacctcata taatgaagga aataagccat gaagatccaa
    11761 gtggagcaag agtagttgat aaaacttttg tacctaaaaa aattaataat ataatagata
    11821 aaaaaactgc tatgagagtt tcagaagctt tagaaaaaac tgttcatttt ggatctccaa
    11881 agagagctta tatagaagga tatgggattg ctggtaaaac tggtacagct gaaaaggtta
    11941 aagctagtgg aggatatggt gcagggtatg tagcgtcatt tgctggattt gctccatata
    12001 atgatccaca agtttcaatt ttaatatctg tagataatcc aaagggggag tactttggag
    12061 gattagtagc agcaccatta gctcatgatt tatttagcga tatatttaat tatattgaat
    12121 tagatagttc agatttagat aaaaataaag taaaagaaga gattgtacca gatgttagag
    12181 gtatgaattt agataaagct aaatctattt tagataaaga caatattaaa tattctgtag
    12241 aagatgctgg aaattctgta gttgatatga atcctaagcc aggatataca attaaagagg
    12301 gagatgaaat taaactttac actaaaacta cttcaaatta taataaagat gttgtagtac
    12361 cagattttaa tggactttca atggaaaaag caaaggaaat tttaaacaaa attggcttaa
    12421 agggtatttt tgcaggagag ggagttatta aggaacaaag tgtagctcaa ggtgatgtag
    12481 ttaaaagtgg tacatcaata gaatttaaat tagataaaaa ataattataa ttaaaaacta
    12541 gcatgtgagt aatttcacat gctagtattt tgtgaattta acgcttgttt attgcatagt
    12601 atttaaggag tgagaaaaat gattcttaaa agtttattaa agggcttaga ttatgaagtt
    12661 ataaaaggta atgaagagtc aaaagtacaa aatataagat atgataacag aaaaatagaa
    12721 caaggggatg cctttgtttg tgtaaaagga tttaaagtag atggacattc ctttattggt
    12781 gatgctatta aaaaaggagc taagacatta atagttcaag aagatgtaag tgttcaagaa
    12841 gacataacaa taataaaggt tagagacact agaaaagctt tagcaataat gtcttcaaat
    12901 tattttggaa atccaaagga taaacttaaa ataataggaa taacaggaac taatggtaaa
    12961 accacttctg cttttattat taaatctatc ttagaaaagg caggatttat gacaggttta
    13021 ataggaacta tagcaaacta tataggaaat aaaaaagtag atgcagtaag aactacacca
    13081 gaatcttacg agcttcatga actatttaaa aatatggttg atgctggagt agaatattgc
    13141 gttatggaag tttcatctca ctctctagag ttagatagag tatatggaat tcaatttgaa
    13201 gaaggaattt tcactaattt aactagagat catttagatt ttcataaaac ttttgaaaac
    13261 tattataatg ctaaatttaa gctttttgaa agaagtaatc atagtatcat aaacttagat
    13321 gatccatatg gggctaatat agttaaggat atagaagaaa gaggagtaaa gactaaggtt
    13381 tctacttttt caattgaaaa agagtcagac tttaaggctt ttgagattaa aagtcactca
    13441 aatggttcag aatttaaggt gaatttagaa gaggtagaag atttttctat aaacattcct
    13501 ggagaataca atgtatataa ctcattaggt tgtataatat gtgcatataa cttaaatatc
    13561 ccaatgaata aaattaaaga gggattaagt gatgtggtta taccaggaag atgtgagtta
    13621 gtagcaaagg agaaaaattt accttatagc ataataatag attatgcaca cacaccagat
    13681 ggattagaaa atattttaag cacagtaaaa gcttttacta gaaatagaat gatttctgta
    13741 tttggttgtg gaggagatag agataaggtt aagcgtcctc aaatgggtaa aataggatgt
    13801 gaattatcag atattgcaat aattacttca gataatccaa gaagtgagga gcctatggat
    13861 ataattaatg acatagtaaa accacttaat tatgataact ttgttattga agttaataga
    13921 aaagaagcta taagaaaagc aatgaatatg gctttagagg gagatgttat tgttatagct
    13981 ggtaaaggac atgaaacata tcaaatacta aaggatgaaa caatacactt tgatgaaaga
    14041 gaagttgtat atgatatttt agaaggcaag tagaggtgtt aaaaaatgct agagttaaat
    14101 ttacaagaga ttgtaaaagc tactaaggga gctttattaa aagaagctta tgtaaaagaa
    14161 ataaaggctg tatcaacaga tacaagaaaa attgaagaag gaactatgtt tattgcctta
    14221 aaaggggaaa attttaatgg aaataactat gttttagagg cctttaataa aggggctaaa
    14281 attgctatag ttgatgaagt taaatgtgac ttaaatgagt taaaagaaga tgtagcctta
    14341 attaaggttc aaaatactag aagagcctta atggatttag ctaagtttta tagagaaaaa
    14401 ttaggattaa aggttgttgg tattactggt tcagcgggaa agacttctac taaggattta
    14461 gtggctgctg ttttaagtga taaatataag gtttttaaaa caaagggaaa ctttaataac
    14521 gaaataggat tacctctaat gatattagaa ttagatagca catatgatgt ggctatttta
    14581 gaaatgggaa tgagaggtct tggacaaatt aaagagcttg cagaaatagc atcacctgat
    14641 tttggtatta taactaatat aggtatttct cacatagaaa tattaaaaac tagagaaaac
    14701 atattaaaag ctaagatgga aatagcaact ttctttgata aaaacaatac ccttgttgtt
    14761 tgtggaaatg atgacttttt aggagcctta cctaatgctg aatacaaaat agttaaaact
    14821 ggagttggag aaaattttaa gataggagct aaaaatatag ctttagaaga actttcatct
    14881 aaatttacag tttatgatgg agaaaaagaa gaagagtttt ctttagatat gccaggagaa
    14941 cataatataa gcaatctaat gttaggaatt gctatagcaa aagaattagg tgtttccttt
    15001 gaagaaatga aaaggggatt aaaaaatatt gaagctactt caatgagatt agagcttata
    15061 aaaaaagatg ggttttcaat tttgaatgat tgctataatt caagtccagt agctgttaaa
    15121 tcagctatag atgttatgaa aaacatagaa ggaaagagaa gaatagcagt tttaggaaca
    15181 atgagagaac taggacataa atcagaggaa gctcatgagg aaataggtaa atattcaaaa
    15241 gaaaatggaa tagaaaaagt actttgtttt ggtgacttct caaaaaatat taaagaagga
    15301 tatggagaag gatgcactgt atatgaaaat aaagaagaac taataaaaga tttacttaac
    15361 attatatgtg atggagatat tatattagtt aaagcatcta gaagtttaaa atttgaagaa
    15421 ataactaaag cattattaga aaagtaaatg gaactaagga ggtgaagctg cttaatagtt
    15481 taagtagcag aattttatgg aaattttaaa gtcaatgata gatccaaaga tagtaatggc
    15541 aatagtaata agttttatag tagcatcaat attaggacct attataattc cactactaca
    15601 taaacttaaa tttggacaaa atataagaca agagggacca aagagtcact taaaaaaagc
    15661 aggaactcca actataggtg gattgatatt tatttttgca actattataa caatgtttgt
    15721 tatggttgga aatccaacag atgaggctat gatagcttta tattccttcg taggatttgg
    15781 ttttgtagga tttttagatg acttactaaa gataattaag aagaaaaatg aaggattaac
    15841 atcaggacaa aaaatgattt tattattaat aatatcagga tttttaactt ggtatgctta
    15901 taaatatata ggaactagca ttaatatacc atttttaaat gggcaaatta acttaggatt
    15961 attctatata ccggctgcaa tgttctactt tgcaggggta acaaatgcgg taaacttaac
    16021 agatggatta gatggattag ctacatcagt tactgtttta gtaactacat ttttagggat
    16081 aattagttat aatttaggac acataagttt agcaatattc tgtgttgctt tagcaggtgc
    16141 attacttgcc ttcttaagat ttaatgcatt cccagctaga gtatttatgg gagatacagg
    16201 ttctcttgct ttaggaggag ctgtagcgat ggtggcatta atattaaaaa tgcctttaat
    16261 actagtgtta ataggaatta tctatgtaat agaaacactt tcagttatat tacaagttgc
    16321 ttcattcaag cttacaggta agagaatatt taaaatggca cctatacatc accattttga
    16381 acaattaggt tggagtgaga caaagatagt ttctgtattt tctataataa cagtagtatt
    16441 ttgcttcatt gcttttgctt cactttagaa aattcctaaa ataggaggtt tttaaatgaa
    16501 gaataaaaag actactaaaa agagtaatat taacccatta gattatggac ttttatatac
    16561 aatagttatt ttattagcta ttggggtggt catggtttat agtgccagtt catattttgc
    16621 tatggtaaac tataatgata gtacagcttt tttgaaaaaa caggctttgt ttgcagcaat
    16681 tggttttaca gccatgatat ttatatcaag atgtgattat cataaactta aaaaattaac
    16741 aggaatacta tttgttataa ctcctatatt attggttgtt gtttatttat ttccagcaac
    16801 aaagggagca caaagatgga taaaattagg tcctttttct ttccaaccat cagaacttgc
    16861 aaaatatgca gtagttataa ttttagcgaa tataattact aataaaggtg agaagattaa
    16921 ggaattttgg aaggggatag ttccttgttt tatagtagga ggcggatttg cagctcttat
    16981 acttgctcag aaaaatctta gtatagcagc tgtaactggc tttgttactt tcataatggt
    17041 ttttgttgca ggagcaagaa aaagatttat gtttggagta ataactccac tgattttatt
    17101 tgcaggttca ttctttactt tatttgagga ttatagaaga agaagacttt taaactttat
    17161 aaatccttgg aaagatccag ctggtgatgg atatcagctt atacaatcct tttatgcttt
    17221 aggagcagga ggagttacag gacttggaat aggacaatca agacaaaaaa ctttatatat
    17281 gccagaacct cataatgatt ttatatttgc aataatagga gaagagttag ggttaatagg
    17341 gtgtacagta gtaatattat tatttgtaat atttgtttat agaggaataa aaatagcaat
    17401 gaatgctaaa gatgaatatg gaactttatt agcagtaggt attacttcaa ttataggatt
    17461 acaagctata attaatattg cagttgtaac aggttcgatg ccagttacag gagttccact
    17521 tccatttata agttatggag gaactgcact tgtatttaac ttaatggcta tgggaattct
    17581 tcttaatata tcaagacaaa gaaataaaaa gattgattaa aggcgtttta tacgccttta
    17641 tttttttgaa gtttatgcaa aattttgata gtaaattttt actattagta atattttctt
    17701 aaaaacttaa tgaaaaaaat atagtaaaat gatattattg ttattgaaat gtaaaataaa
    17761 gagataagga gcaatatgag tactaatata aatgaatata taaaaaaaaa gaaaagaaaa
    17821 aagacaagaa agaaaattat cttagttagt ataacccttg ttgttatatt agggttaatt
    17881 ttaattaaga caccttattt taatgttaaa aaggttaatg ttaataataa caatgttata
    17941 actaaagaaa gtgtaatagg agaaaatgat attttaaacc agaatatatt tttgcttaat
    18001 acttctgctc ttaagaaaaa aatattaagc aatccatatg taaaaagtgt caaaatatca
    18061 agaaagcttc cagatcaatt gattataaat atagttgaaa gaaatgctac atttatggtt
    18121 aatgatggtg ctgattttta tgttttaaat aaaaatttag taataatgga aaagaaaaac
    18181 tcagctgaag gtctacagtt gcctacagta actgggttaa ctgtagaaaa tagattttta
    18241 ggggaaccta tgagtacaga taaggataaa gttgaggttt taaaagagat tggagaagct
    18301 ttaaataaaa gtaaaataaa agtaagctct gtagatatat ctaacattaa caatattatt
    18361 ataaataagg gagaggttaa aatactttta ggtaactctg ataaattaag tgataagtta
    18421 actaagatgg ttaatatatt aaatgatcca gcaggaaatt ttgaaaaggg atatataaac
    18481 ataagcttta atgggaatcc tgtaatatat aaagaaaagt aaggaggagt gctttttgaa
    18541 gaagttgtct tcacaaatta ttataggtat agtttgtgct ttgttagggt ttttattaac
    18601 ctaccaattt aaaattataa ataataaaga aaaaaatata atagaaagtt ataaccaaaa
    18661 tgatataatg gcagagatag atggccttaa aaaggagaaa gaagagcttc aaaagaataa
    18721 tgattcttta aataagaggc ttaaagaaat ggaggaggca gcagcatcta aaggggatgt
    18781 aaataataaa ataaaagagc aactagataa aacaagaatg attttaggaa ctgaagaggt
    18841 taagggtacg gggataattt tatctataac acctaaatct ccattgctta cttcaaagga
    18901 tcctgaatat ataagtgaaa aggagataat tcacatatta aatttattag ctttttcagg
    18961 tgcagaagct gttgcagtta atgatattag aataactcct cagacaggaa taaagaatgc
    19021 aagtaatttt atatggatag gaaataatga aagaatttct ccaaaggaga agctaataat
    19081 aaaggttatt ggtaaccaag aaaacttaat aaaaggaatt gatttcccag gggaaatgga
    19141 ctatggagta ttaggaggca attatgatta taaatatgag aagaaagatt caattataat
    19201 acctaagact actcaaaacc tatcttctga atatttaaaa aaggttgatt aggaaggtga
    19261 tttaatgata gtatttattg ggcttttagt aggtataata ataggagtta tatttccagg
    19321 aagcatacca gataagtttt ccccatattt atctatagcc atagttgcat gcttagattc
    19381 tgtttttgga gctataaagg caagtttaaa gaaagaattt caagctgatg tttttatatc
    19441 aggattcttt ggcaatgcag ctttagcagc tttattagct tatctaggtg ataagcttgg
    19501 aataccacta tactttgcag ctgtaatagt atttggtagt agaatattta ataactttgc
    19561 agctataaga aggatattgt tagacaaaag caaagaacga taaagaggtg attctttgaa
    19621 aaatagagaa tttactattt ttatatttac ggcttcagtt atagttggac ttttaatatc
    19681 tatgaacata agttttgatg gaatagaaac tacaaaggtg ttaaatgcta aggaatatca
    19741 agatgcatat aataaacgca ataatcttta taatgaaata tcaaagctaa atgaaagtaa
    19801 tagagaatta caaaagaaga taaataatta ttcaaaaaat gatgaggata gcaataaaat
    19861 ttcaaaggat atgatagaag agttaaataa taatttaatg ctttctggat tagaagaggc
    19921 taaaggacca ggtttaaaaa tagttttaga agatggtgga gaagctttta gtggagaagt
    19981 tgttgatgat tttatgagac ttttaagaac cattcatgat gatgatatga taaaagttat
    20041 aaatgaactt aaagctgcag gtgcagaggc aatatctata aataatcaaa gaataatata
    20101 taacacagag atatattgtg gaggtcaatt tttacgaatg aatggagtta aaatcccagc
    20161 tcctttttat gttaatgtta taggtgatcc tgaaaaaatg gagaatcaaa ttatgagaga
    20221 tggaagttat attaaaactc ttataaatag aggaataaag gttaaaataa ctaagagtga
    20281 agatataact ataccagcat atataagtga tgtgaattct aagtatatgg aacctaatac
    20341 aaaataaaga actatgtttt gtaaaaaatt tgtacaaaaa agaaggattt tctattccta
    20401 taaagaagaa tataatgtaa tatataatta aaggagacag taaaagtgga tataaaagaa
    20461 aatttaacaa aatttacaaa aaagattccc agtaacgttc aacttattgc tgtatctaaa
    20521 acaagaacta ttgaagaaat ggaagaggcc tatgaatttg gtattagaaa atttggtgaa
    20581 aataaggttc aagaaataat aagaaaattt gatgagtttc acaaagatgt agaatggcat
    20641 ttaataggac atcttcaaac taataaggtg aagtatatag ttgataaagt tcatcttata
    20701 caatcattag atagtattaa attattaaaa gaaatagaaa aggtatatgg tagacataat
    20761 aaaattgcaa atactttaat tcaagttaac ataggaagag aagaacaaaa atatggtatc
    20821 ttggaagaaa aattagatga acttatagaa gctatagaag cttgcaataa tgttaatgtt
    20881 ttaggtatta tgactataat tccaaaggga acagaagaag aatgtagaaa gtattttagt
    20941 aaaactcata agcttttttg tgaattaaaa gagaaaaagt ttaagaatat aaaaatggat
    21001 atactttcaa tgggaatgtc aggtgattat gagattgcta ttgaagaagg cagcaatatg
    21061 gttagaattg gccaaggaat ttttggaaaa agattatata aataaaatta ggaggatatg
    21121 tgtatgtcaa aagtagttag taaaatgaaa agttttttag ggtttgatga attcgaagat
    21181 gaagatgaag taatggaaga agaagaggta atggaggaag aagaatcatt tgctccagta
    21241 ttatcttcta agaaaaatgg taaggttgta aatattcata cagctaacac agctaaacta
    21301 atgataacta aaccattagt ttatgatgat gcaactgaaa tatgtactgc attaaaaaat
    21361 agaaagattg ttgttataaa cacaacttca ttagagctta gaacagctca aagattaata
    21421 gacttcgttg gtggagcttg ttatgcatta tgtggagaac ttcaagaagt tgaaaaagga
    21481 gtatttatag tatcaccttc aaatgttgaa gtaagcaatg aattaaagag tgaattaagc
    21541 aataaaggaa tgttcaactg ggcttcaaaa taatatgaat aaaaaagatt ttctaaattt
    21601 attaaattta gaagagtttg aagcattaaa actctttgag agatttaatt tagctaggac
    21661 aaaaaatatt tcagtattta ctgatgagtt ttatactcca gatgtatgga gcactcttca
    21721 agaaatgaat ttatttgatg taaatgttga aagttttgga ttttttgagg atggagaaag
    21781 aagagtaata gcctttaata aggaagaata tgatttgttt ccaataagtc ttttaaggat
    21841 tcaatgcaat actaaatttt caaaattaga gcacaaggac tatttaggat ctattatggc
    21901 cttaggcata aatagaaaca aaataggtga tttaatctta agagatgatg gatgctatgt
    21961 tgctgttcat catagtataa ttgacttttt aattaataat ttaaataaaa taagaaatct
    22021 taattgtaaa tgtgaaataa tttatgattt agatgaaaca cctaaaccta gctatgaaga
    22081 aaaaaatata atagtaactt ctaaaaggtt agattgtata gttgcagcct tggggaacat
    22141 atcaagaagt aaagctttag atgctataaa ttcaggaagt gttcttctag actatagaat
    22201 aacaaaagat aaaagcaaag aagtttcaga agattcaaga cttaccataa gaggtattgg
    22261 aaagtttaaa ataagcaata tttttgggac aacgaagagt ggtagattaa aattgacagt
    22321 atttaagtat acataggagt gtgattatat gaaattaacc ccaatggata ttaacaacaa
    22381 ggagttcaaa agggttttaa gaggttattg tccagaggaa gtagatgaat tcttagacca
    22441 aatagtcgaa gaatatgaga ttctttttaa agaaaacact tctttaaaag agaagcttga
    22501 aaattctaat gataagatag agcattatag taaaattgag aatacaattc aaaatacttt
    22561 actattagct caaaatacag ctgagcaagc aaaagctaat gctcaaaagg aagcagatgt
    22621 aataattaaa agtgctaatg atacagcaca aagaatatta gataaggctc acaatgatgt
    22681 tatacaaata aatgatgagt atgatagagt taagcaagaa ttcacaaagt ttagagctaa
    22741 gtttagaagc tttatgaatt tccaaataga tacttttgat gatttagaga aagattttat
    22801 taagaatttt aacataacta agccagaaga tcaaactgaa acagaagaac cacaaggcat
    22861 acagattgat tttgaagaag agaaagaaga aaaaactgag cttaaagaca tctatgaagt
    22921 ggaaacacat tttgatgatg taaatgaaat taaaagtttc tttgctgaag agtagagaac
    22981 tgttaaaaaa tctcactttg attaaggtga gatttttctt tttgtttaat taattgttga
    23041 aatttaatat tttattatat aatatttaag atatagcttt aaatatttac tttagaatgg
    23101 agcaaataaa ctatgagttt aagtaaagaa gtaataaaaa taactgaaga agaaaaagga
    23161 ataagagcag ataaattttt agctcttaaa atagatggag tttcaagaaa ctttattcaa
    23221 gggctttttg atgatggaaa aattttaatt aatggaaaag cagaaaaggg aaaatataag
    23281 ctttcagttg gagattgcgt tgaggtagaa ataccagaac cagaggaatt aaaagttgaa
    23341 gctgaggata ttccattaaa tataatttat gaagatgaag atgtaatagt cgttaataaa
    23401 ccaaagggaa tggtagttca tcctgcaccg ggaaattata ctggaacttt agttaatgca
    23461 ttattatacc attgtgatga tttatctgga ataaacgggg ttataagacc tggaatagta
    23521 cacagaatag ataaagatac ttctggaata ttagttgttg caaaaaatga taatgctcat
    23581 caaaaactag cagaacaatt taaagatcat agcataaaaa gagaatacta tgctattgtt
    23641 gagggaagat ttaaaaaaga aggtggcaca attgatgcac ctttaggaag acatcataga
    23701 gatagattaa agtatgctgt ggttaaagat ggaaagagag cagttactca ttatgaagtt
    23761 cttaaagtat ttaattcatg ttcccttgtt aaatgtactt tagaaacagg aagaacacat
    23821 cagataagag ttcatatggc ctttataggg catccattac ttggagatac tgtttatggc
    23881 ttaaagaaac aaaagaaatt aagtcaagag gggcaagttc ttcatgctaa aacattagga
    23941 tttattcatc ctactactgg aaaatatgtt gaatttgact cagaattgcc agagtacttt
    24001 gaaaaaattg tagagaaatt aggagggtgt tagaatatgc atcttaaggc aagtttatta
    24061 gatgaaaatg ctataagaag agcacttaca agactttcac atgagataat agaaaagaat
    24121 aaaggggtag aggatatagt tcttgttgga attaagagaa gaggatatcc tttagcagag
    24181 agactttcag agtttataga aaagtttgaa ggagttaaaa ttcctgttgc tagtgttgac
    24241 ataacattat atagagatga tttaactaat gttagtgata cgccaaattt aaatgatcct
    24301 aaaatagatg tggatataag agggaaaaag gttattatag ttgatgatgt tttatatact
    24361 tgtagaacag ctagagcagc tattgatgca ataatggatc aaggaagacc agaatttata
    24421 cagttagctg ttttagtaga tagaggacac aaggaattac caataagagc tgactatgtt
    24481 ggaaagaata ttcctacatc aaaggatgaa ataataaagg ttcaaatcaa agagattgat
    24541 ggaactgatt cagttgaaat atatgaaaat taaaactaag gagtaaattt ttatggatta
    24601 taatttaata gccactgcaa cttttggatt agaggctgtt gttgcaaagg aattaaaaga
    24661 gttaggatat gaagacctaa aaactgaaaa tggaagagtt cattttgaag gggatgaaat
    24721 ggatattgcc ataacaaacc tttggcttag aactgcagat agagttttaa taaaagtggc
    24781 tgagtttaaa gctgaaagct ttgaagagtt atttaataaa actgtagaga ttgattggag
    24841 taagtatata cctgtagatg gtaagatgca tgttgttggt aaatctgtta agtcaaaact
    24901 tttcagtgtt ccagattgtc aatcaatagt aaaaaaagcc gtagttaaga gcatgagcag
    24961 aagttacggt caagattggt tcacagaaga tggtccagtt tataaaatag aagttggact
    25021 tttaaaagat gtggttacct taacaataga tacatcagga gagggattac acaaaagagg
    25081 atatagagaa cactcagggc aagctccact taaggaaaca ctagccgcag ctatggtttt
    25141 actttcaaag tggagaggag agcaaactct tatagaccca tgctgtggat caggaacaat
    25201 attaatagaa gctgctatga tagctaaaaa catagctcca ggattacata gaaaattcgt
    25261 ttctgaaact tggccttcaa tggataagga aatttgggat caagttagag aaggagcaga
    25321 gaaatctata aagaaaattc ctttagatat aactggttat gatatagata gttgggtatt
    25381 aagtacagct aaaaataacg taagaaaagc aggattaact gactgcataa ctatagaaaa
    25441 aagaaacttt tttgattttt caactaagaa aaagtatgga tatatgatta caaatccacc
    25501 atatggtgag agaataggtg aaaaagaaat agtttcaaaa ttaaataaac actttggaga
    25561 agttaaagag aaattagata cttgggattt taacattcta acagcatgtc cagatttcca
    25621 aaaagagttt ggtagaaaag ctactaaaaa cagaaagctt tataatggta gacttttatg
    25681 ctactactat caatatttag ataataattt aaagaagtaa tttaaaaaga atataatata
    25741 tataaagtta caaatagaat taaaagataa ctgtataaat tattagttta gtacagaagg
    25801 tattagaatc tatttgtaac ttttatttta ttacttaagt ttttctaaag ttatttcctt
    25861 tggtgaatcc atataaactg tcttgttagt tgagtagatt accattccag gtttagaacc
    25921 agaaggtttc tttacatttt taactaaagt atagtctaca ggaattttag tgccaccttt
    25981 tcctttacta tagaaaacag ctaagttagc agcttcttct aaggttttat caggaatatt
    26041 tgaactttta acaattacgt gagaaccagg tatatcctta gtgtgaagcc aagtatcatt
    26101 cttgtcagca aactttaaag ttaaataatc attttggata ttatttttac caacataaat
    26161 atctattcca tcagaagaaa catagtgata aggcttagat tgtttttctt tctttttgcc
    26221 attattattt cttctaaatc ttatatatcc tgtttctata agttcatttt ttatagcatc
    26281 tatgtcttca tagttatctg caacctgtat gcttgaggaa actgaattta agtatttaag
    26341 ctcatcttca tttatagcta gttgttctaa ggcagattct tcagcttttt ttagtttgtt
    26401 atatttttta aagtaaaatt gaatgttttc agatggagtt ttattttcat caagtgaaat
    26461 agtcaggtat tcttcatcct cactataata atttaaaagg tcaacagatt tatctccttt
    26521 tttaatacta taaatataag aggttaaaag ttcaccttta atcttaaatt cctctttttt
    26581 agaacattct tctaaggttt tttcaagaac cttaattttt tttaagcatc tgtctatatt
    26641 agtattaact aatttttgga tatctgcact ttttgcatgt aatctatctt gtttatcttt
    26701 attagcaaaa aaagcatcta aaagttcact actattatca tacacttctt tagtacaatt
    26761 gtctaaaata gataaatctt taaaataaaa atctataatt ttttcgtttt tgtagaatat
    26821 aatattttgt atatctttaa atatgttatt gaagtaatta catataaaat tgaatatatc
    26881 atcaaaaaca tatgaatctc caaattgtga tttaaataat gaatacattt caagagaaag
    26941 atttttacca acaccagtta gtagctttga aaagatcttt tcatctaatt cattattatt
    27001 tttgcttaat tctatcttta aatcttcctt tgaaaagtca aaaggattta atttttcaga
    27061 tgcaggaggg aatacatagc ttacaccagg gtatagaact ctaaaactat ttttatttgc
    27121 tgttatatgt tttattgatt ccataatttt attatctctc tttcttacaa gagaaatgtt
    27181 actgtgtcta gccattattt ctactactaa agtatataca ctatgaaaac ccatttcatc
    27241 tttatttgct atatcaattg aaacaattct atctgtagat tgttgataga tatcgattat
    27301 tcttccacct gttaaatatt ttctaagtac catacaaaaa acaggaggtt gtaatggatt
    27361 attcttactt attgtagtaa aatgtaatct agggtatttg gaactagagg aaattaaaag
    27421 ttttttattt tcctttccac gtacatttat tattatttca tccttttcag gttgatttat
    27481 tttgtcaatt ctagaattta ttaatgaatc ttttaattca tttattaagt tatataaata
    27541 aataccgtct aaagccatga ttttctttcc tttctaaatt gattgaactt acagttagta
    27601 tacatcttat aataaaaagt atcaataata ggtaatttga ggtgataata tgaagttttc
    27661 aaaaatgcat ggaaatggaa atgattttat agttatagaa gatttaaaca atgagtattt
    27721 aggtaaagag ggagaaattg ctcaaaaaat gtgccataga agatttggaa ttggtgcaga
    27781 tgggatttta atagttagaa agaatgaaaa ttgtgatata gaaatggtaa taataaattc
    27841 agatggatct tatgctgcta tgtgcggaaa tggaataaga tgttttgcta agtatgtcta
    27901 cgagaaggga atagtaaaga aagatgtgct agatgtttta acaggtgatg gagttaaaag
    27961 aatcttttta gaaattgaga atgataaggt aaaaactatc aatgtaaata tgggatttgg
    28021 agactttaaa ccaaagaata ttccagcatt atgtgatgaa gaaataatag aaaaaaaagt
    28081 aagtgtagga aatggaaatt tggaaataac atctttactt atgggtgttc ctcatactat
    28141 tatatttgag gaggaaaaat atcctataga atgtggtagg gatatagaaa agtatgaatt
    28201 atttcctcaa ggaacaaatg ttaacttctg caaagtaatt gatagaaata caatggaagt
    28261 tagaacttgg gaaagaggtg cagggccaac cttagcatgt ggaacaggaa attgtgcttc
    28321 agttatagct gctaataaat taggttttgt agataaagaa gtaaaggtaa tagttcctgg
    28381 tggagaatta aaagttaata ttgaagatga tggagtaaaa atgataggta atgcgtcatt
    28441 tatttgtgat ggcacatatt tattttagag agggaagtaa gtaaatgagg aaagctgctg
    28501 ctttaagttt attagtatta gttattttat caacaaccct tgcaggatgt aattttagta
    28561 aagggtctaa tgaagaagac agtaaaggaa aaaagttaaa gcttccttgt agtattgtgt
    28621 ctacaatttt aggggaagat gttttgtatt acgaagtaga aggtgattca ctaaaaaaac
    28681 ttaatttaga tcttaagggg catatggaag tttatagtga ggaattaggt ttagctgttt
    28741 atagggaatt tgctgataag ggaacaaact taataataac taatggagtt aagaaacata
    28801 agatatcagt agagggaaat atagaagaat taattattag tccaaaggga actaaacttc
    28861 tatatagata taattctggg gataaaatag gatacaaggt tttagactta caaaatttta
    28921 aggaatttga ttttaatgag aatttagcta tttcagggga gaatgttaga tttgttaatg
    28981 aggatcaatt aattctatat ggtgttgact tagaaaaaaa acaaagtggt atatatattt
    29041 ataatattaa ggatggaagc tatactttag aaaagcaaat agttaaggcc tttatagatt
    29101 atattgattt agttgaagat caagttgttt tatatactca aagcagtatt gatggcaaaa
    29161 aaaattgcta tatatataat ttggataata aaaaagaatc tgttatttca aatgaggttg
    29221 gagaaataga atctttatgt aaaataggaa atgtagtgta ttttattggt acaaagggtg
    29281 aagggttaag aagtttgtat tcaattgata taacaaataa taaacttgat agattagtgt
    29341 atgattttcc taaaaatata tcagagaaat caaagttaat tgttgttgga gataatatat
    29401 attttcttgg atttaatgat agtaaagaaa aaaatgcact atatagatac aatctaaagg
    29461 agaagagtat taaacttata gatagcaata aagggcagta tattattata aaatagttaa
    29521 aagttctaga ttattaaaaa attttaataa tctagaactt ttttttgttt aaaaaagaaa
    29581 taccttgtca attattactg atgaggaggt tgatatatga aatataccat taaagatata
    29641 gatatgaagc taatggagaa tatatctaag aatacagctt tagagatgaa cttaattgcc
    29701 ttaaaagaaa gttcaggtaa gatatttttt tatacaagca aggaagaatc gaagaaaagg
    29761 gagtatttat ccttattatt tagagaaaaa gaaataaact ttgtaattgg aaataaggct
    29821 gaaatagaaa ttttaattga agattgttat ggtggaaaaa gtttttctaa ggataaattt
    29881 attgaggaag aattaataag ccttatatct ttaggtatag aaaaaaatac tagtgatatt
    29941 catattgaac ccttagaaga atatgctaac attcgcttta gaatagatgg aactttagtt
    30001 ttagttaaga gaataaagaa aaatctttat gaaaatttag taaataagat aaagttaaaa
    30061 gctgatatgg atataagcga taaattaaga tgccaagatg ggaaggtgtc atttaaaaat
    30121 tatgggtatg attgtgactt aagaatatca agtattccaa gtacttttgg tgaaaagtta
    30181 gtagttagaa ttttatataa gaaaaatgag cttatgaata ttgataattt gagtttaatt
    30241 gaagaacaaa agaataaaat aaataaactt ttaaaattaa aacacggaat ggtggttata
    30301 aatggtccta caggaagtgg aaaatcaaca actctatatg cattattaaa ttcaatagac
    30361 aaggaaggga taaatataag cactatagaa gatcctgttg agtttaccat agaaggaata
    30421 acacaaacaa atgttaatga aaaggttgga ttaacctttg ctaatggatt aaagcatata
    30481 ttaagacagg atcctgatgt tatattaatt ggagaaataa gagatgagga aacggctaaa
    30541 attgccataa gagcttctat aacaggtcat aaagtatatt caaccataca cgctaacagt
    30601 ggcaatgaag tttttaatag attattagat atgggagtag aaggatattt agtaaaggaa
    30661 ggagtaaatt caataataac tcaaagatta gtaagaaaaa tttgtgaaaa gtgtaaagcg
    30721 aaaatagagc ttaaaaatga actagttgag aaattaaaag aggaagagtt aagcttaaaa
    30781 aattactata agggaactgg atgtgaaatg tgtaattgga caggtaataa tggaagaaaa
    30841 gcaatttgtg aaattatatt tccaagagaa tataaaagca cagaggatat tacatgggga
    30901 gaagaactat taaaggcatg ctttacctta gttaaaaatg gagaaatatc tttagaagaa
    30961 tattacttat taaaagaatg tgaggggtta gatgtttggt tttaaagatg aagcttcata
    31021 catatgctta aatttaagca catatgtaaa atcaggaatg cctgtaggac gatcattaga
    31081 tttaataaag aacactgtaa aaggtaagaa atacaaagaa tccctaaaga gaattgagaa
    31141 aaaggtttta aatggtgaag ctttatcaaa ggcttttaag gaggaaggta acttatatcc
    31201 agatttaatg aaggatatga tgaggattgg agaagaaagt ggaaacttag aagaggttct
    31261 agaaaagtgt gctagaaata tattagctga aaaggaaata aagaataaaa ttattaaatc
    31321 tcttagatat ccagtttcac ttcttatagg tttaattatt atgatttttg gttatataac
    31381 ttatattttg ccacagtttg tagatatgta tgagttaagg gaaattgaag gttctaaaat
    31441 tatgacttta ttaaataatt atattaactt tgtagaagaa aatcctaatg ttgaagtaat
    31501 agttttttgt tatttttcct gtgccttaat ttcgctttat ctattattta aagtaatacc
    31561 ctctgaaaaa atcttagaaa agatagaagt atacagaatg tattatgaaa ttagaatatt
    31621 atttttaata agcatgatat taaatagtgg catatcccta aataattctt taagtgtatt
    31681 tggagaaagc ttaagtgatg taaagcttag aaaatatatt aagagcataa atgaagaatt
    31741 aataaaaggg actactttaa gcggtagtat agggaaaata aatgttattt ctcatgtatc
    31801 aaagggattt ttaataacag gagaagaaag tggattgttt agtaaaaata ttgaagagct
    31861 tttaaaaata agagaaaaag atcttgatag ggcattaaat aaaactgttg ggaaaataga
    31921 gccagcaatg tttattatat taggttttgt tatttcagct atgatgcttt tagttttagt
    31981 acctaccttt gagggcatga aatatgtata ggaaaagaaa aggatttacc cttagcgaag
    32041 taataatata catggcaata acttctctta ttttagcaat accaacatca tttatgatta
    32101 taaaagatag ggggtatgaa ttaaaagttg aaggggaaat aagtaagatt catgattttt
    32161 taatggaagc aaaacagtta tctaagaaag ataatttata tggagaaata atttttgata
    32221 ttttagacaa taaattaatt tataaaaatg ctcttagaac tacctcttta aagttaaatg
    32281 aagttaaagt aagttttaat agagataatt taattactat aaatgagact gggatgatca
    32341 gtatagctgg aactataaca ataattgata aaaataataa acaaagacaa ataaccatag
    32401 ttccaggtat atggaaaatt aatgttaagt gatttatgag gaaatatagc tatggaaggt
    32461 aagattaata aaaagaaagg atatttacta gtggatatgg ttttagcatt agcattaata
    32521 tcaatacttt tcttaggaat aggaaaaagt tatagcactt atattaaaaa caactattat
    32581 attaaagaga aaataaaagt ggtagaaatt attaattctt tggctatgga gctaaaacac
    32641 aatatatctt ttgaagattt aagaagtatt aatagtggaa catataaaat aagtacttta
    32701 aagataaatg acttaataaa tagtgatatt aaagaatttc ttagaaaaaa tttaatatta
    32761 cataataaaa atcataatga ttttggatgg actattaata taagtcactt tgatcaatat
    32821 gaaagtatta ttaatataat ttataaaaat gaaaaaccat atataagtga aaataaggag
    32881 gtgaaaatat acaaattttt agaaaaatcc tctcaaaatt tagaaaatca accgcaaaaa
    32941 gaagaagaaa agcatattta ttaattgaac tatgtattta tttaggtgct ttaggaatta
    33001 taaccttaag tttaacaaag aatttgattt tttatgttaa ggaatataag agatttattc
    33061 caaaagagaa agaattaaat tatttgttgg atgcagaaat gtacttaaac cacaggtttt
    33121 gggataatat ggttgaagat ataaagataa ataaagataa aaactatatt ttagtgaaat
    33181 tttggagaga tggtatagta gcagaagata aaatagagaa aataaaaaat aaactatatg
    33241 tttattataa ttatacagga tatggacgaa attactcacc tgcactcata gaagatgttg
    33301 aagaatttga tttgatggaa aaaggaaact tattttatat aaaattaaaa gttaaaaatc
    33361 aagaggagag gatctattgt tatgaaaagg cgtaaaggtt ttgtgtctat agaagttatg
    33421 ttagtttcgc taatttgtag catgatagtt actatactta tggataattc atttcaaagg
    33481 agaaaggaac ttgatagaag ttttaaaata acatttaata atatagataa aaataactca
    33541 gaggaacagt ttttaaagta catgttaaaa gaaaaaattt taaatataga taatattaat
    33601 aagttagatt ttactttaaa taatatgaat gcaacttata ttgaggataa aaaagctata
    33661 catattaaaa ggaaagataa gataagcgga gtttttaagg atacttacta tggtatagat
    33721 tttagaaatg atgatattgt cttttcaaaa aggagaaatt atgagtttgt taataaagac
    33781 cttagtaatt aatgatacaa atgatatttc aaaacttaag gaattaaagg ataagagagt
    33841 aaaaattatt cttttaaatg aaaagatatt tatagaaaca cttagagttg ataaaaagtg
    33901 taatattgaa gaaaaaatag aacagcttat aagagataga ttttttaatt acacaccatt
    33961 agttcattat gaggtattaa aatataacaa aagtttattt ttaatagtat attttattgg
    34021 atgtgatgaa agatttaaaa gtttattata tgaaagaaag aaattttctt taatatttcc
    34081 tgaacttaaa aataagaata tattatcttt taataaagct acttttgaac ttaaaaattt
    34141 aaaaataagt atttatataa aaggtaaatt agttttatta aagagtgtaa aagattctaa
    34201 tattattgaa gtaatagagg aatgtcttaa aagcatagag aaagattttg gagtatattt
    34261 aaaagacttt acttttaaaa ttcagaaaga ttatttaaag gaagaaataa aagaatggtt
    34321 taaaggtttg aaattaaatg agattagagg tgaggagaat ttatatcaaa aaatataaaa
    34381 attttttacc aattgaatat atagaggatt ataacagaaa aataaagaaa aagctaaata
    34441 tatacatatt gcttttatta ttatttaatc tattttttta tgggaaagtt aagactgaaa
    34501 ataataaaat aaaaaattat ttaagtgaaa taaggggagt taatagggag agatctttag
    34561 acaaggaatt tttagaagat ataaataagt taaaggaaat aattaaagat gaaaagatat
    34621 taaagttaaa tctagagaga aaaagttttg aagtagaagt taatagtatt tataaaaatt
    34681 ctcttattaa ttatattaat aaaattaatg gaagagttta tgatataagt ttaaatgaat
    34741 cgaataatac ttatagatta aaaggagaat taaggtgaaa aaattaaaat tagatgtaac
    34801 tttagctact atttttataa ttatactatt agtacaaggt atagagcttt ttcaattaag
    34861 aaattatgaa attccagttt taacaaactc tataggagaa atggatttaa gtacagtatt
    34921 aaaaaatata gaaaataagg attgtactgt tttacaatta gaatataaag agggatggat
    34981 aggtgaagtt ttatttacag gttctttaga ggatttaact aattatgtta acttattaaa
    35041 aaataataat attaatataa aaaattataa aatagaaaaa tcggaagaat taaagtgttt
    35101 tttggagctt aaggttgtat agtcttgtaa atttaatgaa aataattgca tttattatta
    35161 gcttttgata aaatatgatt gttgttttac actttaaata gtaaataagt aacaaggtaa
    35221 ctctagaggg tattaattta tcttaaaggg ttataatata aaatgaatta atagattttg
    35281 gagggatact taatgatatc aggaagtgat ttaagaaaag gaactacttt tgaactggat
    35341 ggacaagtat acacagttat agacttctta cacgttaaac caggaaaagg tgctgctttc
    35401 gttagaacta aattaagaaa cgttataatg ggtggtgtta cagacagaac tttcaaccca
    35461 actgataaat tacaagaagc tataatcgaa agaaaagaaa tgcaatacct ttactcagat
    35521 ggagaattat actacttcat ggatcaagaa actttcgagc aaattccttt aaaccatgaa
    35581 aaagttgaag atgctataaa attcttaaaa gaaaacatga acgctgtaat caaattctac
    35641 aaaggagaag ctttctcagt agaagctcca aactttgttg aattattaat cactgagtgt
    35701 gagccaagtg ttaaaggaaa cactgctact acagctatga aaactgcagt tgttgaaact
    35761 ggtgcaacag ttatggttcc tatgtttgtt gaagaaggaa acactataag aatagatact
    35821 agaactggcg agtacatgga aagagtttaa taaaaaactc gatttaaatg tatttaagtc
    35881 aaatttatct agtgaatata gataaaaata aatttatgct aagcttaaaa agcttagcat
    35941 atttaatata aggaagtgat ttcagtgaag gatattaaaa ataacattag caacttactt
    36001 aaagctatgg ataaaaacac tgagttagat aaagaattta aggattcctt acttaatgta
    36061 atttcatctt tagttgataa ggttgaagag ttacaagtta atgttgaaac tctagatgaa
    36121 aatgtaaatc tactaaatga tgatttaagt ggagtacagg atgaactttt tgaagagcta
    36181 actttagaag agcttgaaga atatgatgat gaatactgtg aagtagtttg tgataaatgt
    36241 cataagccta tatacataga aaaagatata ttaaacagat ctgaaagtat accttgtcct
    36301 tattgtggaa atgaatttga agtttaataa actgtgcctt ataaaaggta cagtttattt
    36361 tttttgctca aaattagaaa atacttagaa tagtttttta tgtaaagtaa taaatttaaa
    36421 tagaagagaa gaagaggtct ccaaaaaaga tttattattt taaaaataat tagaataatt
    36481 tcttatgtaa agtaatagat ttaaatagaa aggagagggg gtactttgaa agagatattt
    36541 gatgtattac cagataagat aaattcttgt ttaaaagata aaaataattt aaataaatta
    36601 caagaaatta gagttaagat tggaaaacct cttaacatag ttttagataa tacagaaata
    36661 atttttaact atgtaataag gaaagaggat gttaaagcca taattcaaaa aataagtaac
    36721 tactctttat atgcctttga agaagatata agacaaggat atataactat ccaaggaggg
    36781 catagagttg gattagcagg tcaatgtgta atagaagata attctattaa aactattaga
    36841 aatataactt ctttaaatat tagagtttgt agagaaatag taggctgttc aaatagttta
    36901 atgaatagct tagtagaaaa taacagggta aataatactt taataatatc tccacctaaa
    36961 tgtggaaaaa ccacactttt aagagatatt acaagaaata tatctaatgg aatttctcaa
    37021 attggtttta agggaaagag aacagttgta attgatgaaa gaagcgaaat agctgcttgt
    37081 tacaatggaa ttccccaaat gaatgtagga atgagaactg atgtatatga taactgcata
    37141 aaaagtgaag gcatgatgat ggctgtaaga gggctttctc cagaggttat tatatgtgat
    37201 gagattggga catataaaga tatggaaggg ttaatgatgg catataattc aggagttagt
    37261 attatagcta ccttacatgg aagaaatgtt gaagaattgt ataggagacc tgtttttagg
    37321 gagattgttg aaaataatat aataaataaa gttgtagttc ttagtggaaa aaaaggaatt
    37381 ggaacaattg agggtatata taatgtttaa cataataaaa ttatctttaa attttctagt
    37441 aggttatgaa aaataagata tctattttca attggtgcta cttaatttat tagaatgaat
    37501 aaaagatata aaaataacaa atctactaga aaagttatct ttaataggag gaggaaatga
    37561 aagttttttt tctgcttatt atagttctac ttagttcttt aattggatac ctatatggag
    37621 aaggatttag aaatagactt tctcaactta gagagttaaa aagagcttta atagattttg
    37681 aaaatgatat tgtttatact tacactcctt taccagagag tatagagagt atagctttaa
    37741 aagctaaaag cccaataaaa gaattattta atgaaatttc ttttaaatta aaaaataatg
    37801 aagtggaaaa tgtatatatg gcttttaaag aaagtataaa tgaacataag aaagaaatga
    37861 acctaaggaa taaggatttt gaaattttat tagatctttc aaagtcttta ggagaaacca
    37921 atgtagaggg acaaattaag atatttaatt tagcaaagga aaagctagat atagaattag
    37981 agattgctga agatgaatgc aataaaaata ctaaggtata taggtattta ggggttgcag
    38041 taggtgcaat gatagctatt tttcttgttt agtctaatag atttttagaa aagaaatttt
    38101 acttttggac tatttaataa gagttaagtt ttagggggag gccatatatg tcggatttat
    38161 ctttgatttt tcagattgca ggagttggta tagtgcttgt tatactagat aaagttcttg
    38221 atcaaagtgg taaaaaggag tatgcaactt tagctaatat agttggagta gtcataatac
    38281 ttaccatgat gattcaactt ataagtagat tattttcttc tgttaaatct atgtttttat
    38341 tttaatagat ttatgaaata tattgagaag gaggtgtcta tgacagtaat ctaaggttta
    38401 aataaagcaa aagattactg gcaaaatgaa atatggatgt agttatacaa gtaataagct
    38461 ttgtactaat aagtttattt ttatatctag ttttaaaaga taataagagc acctttgcag
    38521 tttttcttct tttaggagct ggcatagtaa tttttctttt tgtaatgcct tatgttaatg
    38581 aaataatagc atttatgcag gatatagcaa gagagtctgg aatggattca tcatatattg
    38641 ctatagtcat gaaaattatt gccatagcat atctttccac tttttgtagt tatatatgta
    38701 atgatgcagg ggtcacatta ttaggtaaga aggtagagtt tacaggaaaa atcatgattt
    38761 tattattagc tataccaata atggctaata ttttgaattc tattttaagt ataatgtagg
    38821 agctaagata tgaaaaaatt gataaacata atattattat ctcttatctt tgtattttgc
    38881 tttaacttaa aagttttggc gaatgataac ttagagagtt caaaagaaaa aatagacaat
    38941 gactcaagaa tagaaagatt ttatgattat ataaataatt tagaaacaga agaagatata
    39001 ttaggcaata tgtctgctaa ggaatatata atgaattatt tagaaagtgg agaagatcca
    39061 ataaccttaa agaaaatagg tgcttcaata cttagttata tctttagaga acttaacata
    39121 gttttaaagt ttatcgcttc aatactagta attgctcttt taagtgcttt acttaaaaat
    39181 ttacaggatg cttttaatat tgaagaggga gtaacacaga tagcattttt tgcttgctat
    39241 gccttactta taatgctttt aactaaaagt ttctttatat ctcttaactt aggaaaggaa
    39301 gtcttaactt caattataga ttttatgaat gttattgttc cagttattgt aatgcttata
    39361 gctacctcag ggggagtaac atcagctatc accatagatc ctatagtact aggggcagtt
    39421 tctataaccc ctagaattta cacatatttt ttatttccac taatattagc atattttaca
    39481 ttacagtttg ttaataatct ttctagtgaa tttaaaatag atagaatgtg taagtttata
    39541 aaacaagtag ttatgatatc tcaagggttt atattaacaa tatttgttgg aatattaact
    39601 ttaagaggaa tgacggcaga taccttagat gcagtggctg taaaaacagt aaagtttgct
    39661 gtagataatt ttgtgcctat agtgggaaag gcattttcag atgcaataac cacagtagct
    39721 ggatattcct tagctatgaa aagtgtaata acttctttag gattaatagt tctagttgta
    39781 attgtaattt atccaataat aaaaataggc cttatggcta tatgttttaa acttacctcc
    39841 gcggttattg agcctgttgt tgattcaaaa atttctagca gtgtggaaac cgttggagaa
    39901 gccttattta tgataatgtc atgcataata agtgtcagca taatgttctt tattatggca
    39961 tctatgatgg ctacagcagg taattttata gtaggaggat agaagtaaga tggaactttt
    40021 aaaaaattgg atttcaaccc tttgcgttgt tatagtcata gtatctattg ctcatataat
    40081 tttacctaat tcttcaataa aaaaacatgt taaatttgca ttttcattaa taatactttc
    40141 tgtaatgcta tcaccaataa tagggctttt aactatgaac aaggatatag atgaaactgt
    40201 ttttcaggaa aaaataaatg atgttacaaa atctaatgag gaaaaaaagt cattatatga
    40261 tgaagaagca attttaaaaa gtgttgagaa aaacttagaa aagtcattaa aagatgaatt
    40321 ctatgagaat gagtttgagg ttaatttaat tgggaaaata aattttgatg aagtgaaatt
    40381 taatatagaa aaagcagaaa ttacagtttt agatgaaaaa aaagtaaaaa aagtagataa
    40441 ggttgttgtt ggaaaagaaa aggttaataa agaagagaaa aaggattctt ttttagaaaa
    40501 agttgaaaag tttgttgaaa aggaacttga aatttctcat gaaaatataa ttgtttcata
    40561 tgcctaggag gggatataaa ggtgcatgag ttaaaggaga aaatagctaa tctttttaaa
    40621 cagaaaaata ttactaattt aataatatta cttttattag ttataatgtt ttatttagtt
    40681 gtgtcatatt ttacaggtgt aaacaatata actaaaagtg agaaaactaa tttagaaaaa
    40741 gtatcaaaag aggatatgaa tagtaatagt caaaaagatt cagaagtttt aagttatcaa
    40801 gaaaaacaag aaaaagattt agagaggata ttaggaaaaa taaatggagt aggatcagta
    40861 gatgtagtaa taaactttca aagcagtgaa gtaaaagtac cagctgtaga taattcttct
    40921 caaaaaagca ctacagaaga aactgatagt gaaggaggaa caagagttaa ttcacaagaa
    40981 actgatggag ataaaatagt tatgtcaaat agctcaaatg gaagtgaacc agttatatta
    41041 aaaacagaaa agccagaggt tttaggagtc atggttgtag ctgaaggggc agaagatagc
    41101 aagataaagt atgaaataac aaaagcgata tcaagcttat ataacataag tgttgataag
    41161 gtaaatgtat tagcaatgaa aaaataaaat taaaatttat gggaggttca ttatgaacag
    41221 aaaacaagca gggataattt taacactttt agcattaata gtttgtactg gcgttctagc
    41281 aactagagtt aacaatcaaa ttaaagagag tatgggagaa gttcctacag cttttggaga
    41341 aaatgatgac aatgcaactg aaacatcttc aaattatttt tatgaatcaa gaaatttaag
    41401 agaacaaaaa gattctaaaa ctatcgataa cttaaaagct atagtagaag ataagaacac
    41461 ttcagcagaa caaaaagaag aagctgaaaa agaattaact gaaaaaacta tggcaagaga
    41521 ttatgaaaca agaatagaac ttagtataaa gagcaaaggt tatgaagatg taatatgttt
    41581 tatagatggt gataatgcta aggttgtagt taagactaat gaggagttaa ctcaagagaa
    41641 aatggttgaa atccaagata tagttatgaa tgtatcaaaa gtatatgacg ttgatataga
    41701 aaaaaaataa ttaattgtat tgtaattagt tatttgctat aatgaaccta taactaatta
    41761 agtttcgtga agattagtag gagggttcag ctttatgaat gaaatgaata gagatgaagc
    41821 taacttaggt atagttaata tatctgatga agttatcggt gtcgtagctg gtatagctgc
    41881 atctgagata gacggaatac ttgagataaa tcacaataat agcactggaa taggacataa
    41941 attttcaaag aaaagtttag gtaagggcat taaggttaat gtggagaatg gtgaagcagt
    42001 tatagaaatt ggtgttactg ttcaatacgg aattaagatc cctgatgtag tttctcaagt
    42061 gcaggaaaac gtaagaagga ctgttgaagc tataactgga cttaaggtgg cactagtgaa
    42121 catatatgtg caaaacataa ttatcttaaa agaagaagat aaaaatgaaa tattaaaaaa
    42181 atagtagtca ccctctgatt tcagagggtg tttcattgtg ttaaatcatt aactatatga
    42241 ttaataatat aaattaagac aataatacta aatagagtat tttcaaaaca taggaggata
    42301 taatgaatag agtaaaatca agagaatatt tattacaatt agcttaccaa atggaaataa
    42361 cttcagaaac tgcattagaa actttcaatt cttttatgga gaacgaggac atttctaagg
    42421 atgatttaga tctagcatac ataaaatcag gattattagg aatagaagaa aataaggaaa
    42481 agttagattc tttaatagaa agtcaacttg taaaatggaa attaaacaga atttcaaagg
    42541 ttaacttatc aattttaaga atttctacat atgaaatatt atttgcagag gatgtaccag
    42601 gaaaggtatc aataaatgaa gctattgaat tatgtaaaaa atattcagat aataaatcag
    42661 tttcatttat aaatggagtt ttagataaag tatataagaa tattaaatag gggctaaatt
    42721 tatttttact agaatgataa gatttggagg ggcaggtaat ggataaaatt ttaagcggaa
    42781 aaacagtggc tatagacata aaggggcaaa ttaagaatta tacagaggaa ttaaaagctt
    42841 ctggaaagtc tttaaaaata tcgtctatac tagttgggga tgatggaggc tcagtatatt
    42901 accaaaactt ccaagaaaaa ttagctaata atttaggaat agactttgag aagataaaat
    42961 tagatgaaag tatttcagaa gaaaatctga aacttaagat agaagagtta aataaagatg
    43021 acagtgtaaa tggaataatg ctcttattac ctttaccaaa gcatattgat gagagagtag
    43081 ttacaaattt aatagatgca gataaagact tagattgtct ttctgaggta agtgtaggta
    43141 gattctataa gggtgagaag tgttttatgc catgtacacc aaacagtgta ataactcttt
    43201 taaaagctta taacattgaa attgagggaa aagaagttgt tattattgga agaagtaata
    43261 tagttggtaa acctcttttt caaatgtttt taaatgaaaa tgctacagta acagtatgtc
    43321 attcaaaaac taagaaccta aaagaagttt gcaagagagc tgacatatta gtagtagcta
    43381 ttggaagggc taatttcata gattcttctt atgttagaga aggagcggtt gttattgatg
    43441 taggaactag tgaggttaat ggtaaaataa cgggagatgt taattttgat gatgtttatg
    43501 aaaaggcatc attaattaca ccagttccag gaggagtagg ttctttaact acgactcttc
    43561 ttttaaaaaa tgtttgtaag gagttagatt agaatgaaac taaagacatt atctgttggc
    43621 gaagttaata actacgttaa aaagttagtt gaaaatgact ttatattaaa gaatcttaat
    43681 gtcaaaggtg aaatatctaa tttaaaattc cattcaagtg gtcatattta tttttcattg
    43741 aaagatgaaa atagcaaggt taactgcatt atgtttaaaa ataatgcagt taatttagat
    43801 tttagactag aagaaggaat gaaggtagaa ataaaggcta gattaggtgt ttatcataaa
    43861 gaaggaactt atcagcttta ctgcgaaaat attaaaaaag caggaattgg agaacttttt
    43921 gaagaatttc ataagttaaa aaaagaactt agtgaagaag gaatttttga tgaaaaatac
    43981 aaaagagcct taccaaaatt ccctaagaga attggaataa ttacagcaaa gactggagca
    44041 gcagtaaggg atataataaa cgtaatacaa agaagaaata aatctttaga tattatttta
    44101 tatccagcta aggttcaagg agaaaatgca gcagattcaa taatcgaggg tattagatat
    44161 tttaataatg agaaatcagt tgatgttatc atcttaggta gaggtggagg atttattgaa
    44221 gaactttggg cttttaataa tagagactta gcatatgaga tatttaatag tagaatacct
    44281 actgtatcag ctgttggtca cgaagtagat tttactatta gtgattttgt aagtgatatg
    44341 agagcaccta caccatcagc agcaggagag ttagtatcac catcattaca agaaatgata
    44401 aatgatttat taaataaaaa agagttctta catagagcta ttgacagaaa atttttaaat
    44461 gcaaagagag atgtagattt attatacaaa ggattaaaag gtaataatcc taaagatata
    44521 attgaaaaaa gaataaaaga agtaaattcc ttagaagaaa agttaaactt tttaggaaaa
    44581 agaaaaattg ataaggcaaa agatgaactt attgctttaa atagcatatt acaaacctta
    44641 aatccattaa acaccttagg aagaggatat tcagtaataa tggataaaga agataaagta
    44701 attaacaacg taagtgaatt aaaaaagaac gatatggtaa aagtgataat gaaggatggt
    44761 tccgttaata tagatataaa aattattaat gaataaggag caaagaggga taagatggct
    44821 agaaaagaaa ctaattatga aagtatggtt agtgaattaa atgaaatcgt aaagcaactt
    44881 gaaaatggag atttaacttt agaggagtct ataaaaagct atgaaaatgg cgttaaaata
    44941 gtaaataaac tatataagaa gctaagcact ttagaaggaa aaataaaggt tgttgaagat
    45001 gaaaaagagg aagactttgg aggatacagc aatgaatatt aatagcttaa aagaggaagt
    45061 tgatcaatct ttaaaagctt attttgataa agatagagaa tacaataaag ttttatatga
    45121 ttcaatggca tatagcgtaa acgtaggagg aaaaagaata agaccaatct taatgctttt
    45181 atcttactat atttataaaa gtgattataa aaaaatatta acacctgcaa tagctataga
    45241 aatgatacac acttattctt taatacatga cgatttacca tgtatggaca atgatgattt
    45301 aagaagagga aaacctacaa atcataaggt ttttggagaa gctattgctg ttttagctgg
    45361 agatgctctt ttaaatgaag ctatgaaaat tttagttgat tattctttag acgaaggaat
    45421 aagtgcatta aaggcaacta aaataattgc agatgcagca ggatcagatg gaatgattgg
    45481 tggtcaaatt gttgatataa taaatgaaga taaggaagaa atttctttaa aagaattaga
    45541 ttatatgcat ttaaagaaaa ccggagaact tataaaagct tcaataatgt ctggggctat
    45601 tttagcggaa gcttcagaga gagatattaa aaaattggag gattttggat ataagttggg
    45661 cttagcattt caaataaaag atgatatatt agatgtagtg ggaaatgcaa aagatttagg
    45721 gaagaatgtt cacaaagacc aagaaagcaa taaaaataat tatataacca tttttggatt
    45781 agaagagtgt aagaaaaaat gcgtgaatat aactgaggaa tgtatagata tattaagttc
    45841 aatagaagga aatatagagc ctttaaaagt tttaaccatg aaacttttag agagaaaatt
    45901 ttaataatga aggatttggt tttaatgagt gaaattctac agaaaataac tgatcctaaa
    45961 gaaataaagg atttagatga aaaagaatta gaaatgttag ctaaagatct aagagaattt
    46021 ttaatagaaa gcgtttcaaa tactggtgga cattttgctt caaacttagg tgttatagat
    46081 ctaacagtag ctttatttaa aaattttgat tttagtgaaa atagaataat atgggatgta
    46141 ggacatcaat cttatgctta taagatatta actggaagaa aagataaatt taatacttta
    46201 agacaatatg gtggattgtg tggttttcct aagagaacag aaagcgaata tgattttttt
    46261 gccactggac atagtagtac atcactatct tcagcagcag gtatggctag agctcagaag
    46321 attcttggaa aagataataa ggtcatagca gttataggtg atggagcctt aactggaggt
    46381 atggctttag aagccttaaa cgatattgga tatagaaaag ataatcttat aataatatta
    46441 aatgataatc aaatgtctat atgtaaaaat gttggaggac ttgcaaccta tttaaataag
    46501 cttagaatgg gtgtaggtta taataaatta aaatcagata ttggatcaac tttagataca
    46561 acttcttttg ggaaaagagt aaagaactct ctttcaaaat taaaagatgg tattaaaaaa
    46621 attgttgtac caagtatgta ctttgaggat attggattga aatattttgg catagtagat
    46681 ggacataaca ttagagaatt aaatgaagtt ttaagcatag ctaaaagtat aaaaggacca
    46741 gttataatac atacagttac taaaaaagga aaaggatatg aattagcaga aaaaaatcct
    46801 aataaatatc atggagtatc tccttttgat ttaggagaag gagtgatttc aaagtttgca
    46861 aatagaaatt attcttctac ctttggagaa gaaatgatta aattagctaa aaatgatgac
    46921 aaagttgttg caattacagc tgctatgcca gatggaacag gattaaaaga ttttagagaa
    46981 gaatttcctg atagattttt tgatgtaggt atagcggaac aacatgctgt tacattagct
    47041 gctggaatgg cggcagaagg tttaaaacca ttttttgcag tctattctac tttcctacaa
    47101 agagcttatg accaagtttt acatgatgta tgcatacaaa atctacctgt tacactttgt
    47161 ctagatagag ctggcttagt tggagaagat ggagaaactc atcaaggtat attcgatatt
    47221 tcatttttat ctccaatgcc taatatgact attgttgcac ctaagtgtat agatgaaatg
    47281 gaagttatct taaaatgggc aagtaatttt aatgcacctt tagctataag atacccaaga
    47341 ggtggagata ttaatgttaa tttaaaacca ttaagtaaaa tagaatatgg aaaatgggaa
    47401 aaggttcaag agggagagaa aatagcaata gttgccactg gtaaaatggt tcaacatgct
    47461 atgattgcta cacaaaaaat aaaagaagaa aaaaatatag atattttaat tataaatgca
    47521 acctttataa aaccaataga taaagaatta ttaaattcct tgtcaaagga tggatttaag
    47581 attgtaacta ttgaagataa tattaaaaaa ggtggctttg gagaaggcgt tctagagtat
    47641 ttaaatgaaa ttggacatga agaaaaaatt gttacattag catttaatga taagtttata
    47701 gaacatggta agcctgatat tttatataaa attaatggat tagatgcaga gggaataaaa
    47761 aacacattaa ttgaattact ttaaaggaga aaattatggc agaaaaaaaa gagaaaaaag
    47821 aaagacttga cgtacttctt gttgaacaag gtattgctga gtcaagagaa aaagctaaaa
    47881 gatatataat ggcaggtatg gtattcatag gagaaaagag agttgataag gctggagaaa
    47941 aggttcctgt aagctcaaat atcatattta gaggagaaaa actacctttt gttagtagag
    48001 gtgggtttaa acttgataag gcagttaaat cctttggaat agaccttaaa ggaaagagat
    48061 gcttagatat tggagcatca actggtggat ttactgactg tatgcttcaa aatgatgctt
    48121 caaaagtatt ttcaattgat gttggttatg ggcaatttgc ttggaaactt agagtagatc
    48181 caagagttgt ctgtatggaa agaactaacg taagatatgt aaccccagag caaataggag
    48241 aattatgtga ttttgcatca atagatgtat cttttatatc attaacaaca gttttgccag
    48301 ctgtacttaa tcttttaaat gataagggag aagttatggc tcttattaaa cctcaatttg
    48361 aagctggtag agaaaaggtt ggtaagaagg gtgttgtaag agaagcttct actcataaag
    48421 aagtaattaa gaaaattgtt gattttgctt tatcacacaa attaaatata ttaggtcttg
    48481 atttctctcc tataaaagga ccagaaggaa atatagaata tttaatatat ttaaagaagg
    48541 ctaacaatac agaagagttt gattataata ttatagatga aattgtagat aaatctcata
    48601 gtgatttata aaaattaaat tattaaataa gagatattaa ttttataatt actttagaga
    48661 aactgcatag ttaaagtaaa ggtgtgtttg gggcatgaga aatataggaa ttataattaa
    48721 taaagaaaaa gataaagaaa ataaaatttt aaatttggtt attttaaagg ttaaagaata
    48781 tctaaatcct gatgaaataa aagttataga ccaattttat aagggagatt ataaagattt
    48841 aatgtcttta gatcttttaa tagtccttgg tggagatgga acgcttttag gtgtggctag
    48901 aaaattctct acagttatag atactccaat tttaggaatt aacattggaa atttaggctt
    48961 tttagttaca gctgagataa gcgaattaga tgaagcttta tatagaataa aggttggtga
    49021 ttataaggta gaggaaagaa tgttactttc ttgtactata gaaggggtaa cttgttcaga
    49081 tgaaagggct ttaaatgata ttgttgttgc tagaggaaca ttatctagaa tggcacaata
    49141 tgaggtcttt ataaatgatg aactttatgc tacttttaaa ggggatggag ttataatttc
    49201 tactccagtt ggatcaacag cctattcttt ttccgctgga ggtccactta taatgccaga
    49261 tttacaaata gtatcaatag tccctatatg tcctcataca cctaattcaa ggcctatgat
    49321 aatagatgga aataacaagg taagagttaa gccacttatt aatgaatcag atgtctttgt
    49381 tacaatagat ggacaaaaag cattaaagtt agaaaaacac aatgaagttt taataaaaaa
    49441 agcaaaagag ttttttagaa taatttcatt tgataataaa agttatttta aggtactaag
    49501 gaaaaaattg tttaagatag aataaatatt atgaaggtga gttgaagatg aaatctaaga
    49561 gacattctaa gataattgaa attataaaat caacagcgat agaaactcaa gaagagttag
    49621 cggaagaatt aaaaaaagct gggtttgatg ttactcaagc tacagtatct agagatataa
    49681 aaaatttaaa acttgttaaa aaacaagata ataaaggaca ctatagatac acaatcatag
    49741 aagaagaaaa aaatgaaatg gttgatagat taagtaatat attagaaaat actgtaactt
    49801 cagtagaaaa tgttgagaag atggttgttg ttaaaacaat atcaggttca gcatctgcag
    49861 cagcagaagc aatagatact cttaatttag gtgaaattgc tggtactata gcaggagata
    49921 acacaatatt tatattagtt agaagcttag aaaaagcaga aactctagtt tctaggatac
    49981 tagaaatgat agaaggatag ttaaggagga gaagtatgct tttacaatta actataaata
    50041 actttgcttt gatagaaaaa gcatctttag attttaaaga aggattcact attttatcag
    50101 gagaaactgg agctggtaag tctattctta tagatgcgat aaattatgtt caaggaagta
    50161 agtttaataa ggacttaatt agaactggag aagaaaagac ttttgtagag gctatatttt
    50221 caatagatga taatgagagg cttaaagaaa ttttagatga tttagaaata gagtatgatg
    50281 atactttaat aatttctaga gaaaccttta taaacggtag aagtaatata aaggttaatg
    50341 gaagaagtat aatagtagct actttaaaaa aaataagtag cacacttcta gacatacatg
    50401 gtcaacataa taatcaaaat ttattaaata aagaaaatca tataatgtat ttagataatt
    50461 ttggagattc taagttaagt gaagatttaa aagaatatgg agaaaagttc ttagagctta
    50521 aggatataga aaaaaaaata aatgaattaa gcaatgaagg aaaagatgaa aaacttttaa
    50581 attatttaga atatcaatta aatgaaattg aagaagctaa gcttagaaat ggtgaggaag
    50641 aggaattaac agaaagattt aatgttttat ctaatgcaga aaaaataaaa aattcactgg
    50701 gaatatcata taatctatta aatggtattg aaagcagtgt ggtggattct ttatcagtag
    50761 taaataggga gctttcaaat gttgaagagc atttagaaaa aatcaaaaat ataaattcta
    50821 aaataatgga aatgtactat gaaatacaag aaatggctag agaaattaga gatatatgtg
    50881 atgaaagtgt ttatgatggt aatgaattag aagaaataaa tagccgtatg tttaagattg
    50941 cttccttaaa gaagaagtat ggaaatagta tagctgaaat cttagaatat aaaaataata
    51001 ttttatctca aataaataat attaaaaact cagaaaaaat aattcaagat cttttaaatg
    51061 aaaaaagtaa agttgagaat gttttaagtg aaatagctaa tagaattcat aataagagaa
    51121 tagaactttc aaaagtttta gaagaaaata tccataaaga actagcctat gttggtttag
    51181 gaaaatgtag atttgaagtt cttattgagg aagatgaaac ctttaacttt aaaggtaagg
    51241 acaaagtaca atttcttata tcaacaaatc ctggtgaacc attaaagcct atggaaagaa
    51301 tagtttctgg aggagaactt tcaagaataa tgttagcttt aaaagctgtg tttatagata
    51361 aggataagat tcctacagtt atatttgatg aaatagacac aggtataagt ggaagagtag
    51421 ctcaatccgt tggagaaaag atgtatgaaa tatcaacaaa acatcaagtt ttttgtatca
    51481 cacatcttcc tcaaatagca agtatgtcgg acaatcatta tatggttaga aaaaaagtaa
    51541 tagataataa aactttttca aaggttgaac ctataactta taatcaaaaa attgaagagg
    51601 ttggaaaaat gctaggtggc gttgaaatga caagcaatac attattaaat gctaaagaga
    51661 tgatagagct agctgataca aaaaaagaaa caattaagac ttttcataca taataaagtg
    51721 atttttaata caaaatagaa ctgttaaaca gttctatttt tttttatttt aaaactaata
    51781 tgagaatttt agtatatttt aaattgattt ggctagaata acaaagttaa tacaggatat
    51841 tatttttttg aaaggggcaa attaagaata gaaaaggagg agatagttaa tgaaaaatat
    51901 gaacaaaaga ataaagatta tgtcaataat aatgatgtct ttaatattac ttttatcctc
    51961 tgtaacattt gcaagggatt attgtgagag taataatgtt tttgcaagta gcaatttcta
    52021 ttctttaaat tataagagtg gtaatgataa aaagtttaag aacagatatg gcgtagctct
    52081 tgtaaatagt gaattagaaa aaaaagatat agagctttat gctggaggaa attcagtagg
    52141 tgtaagggtt tcaacagatg gtgtattagc agtaggttat tcaagtttag caatgggaga
    52201 gggagaaata gagagtccag cgcaaaatgg tggaatacaa attggtgata gacttataag
    52261 tgtaaatgga gataaaataa aaaattcaaa agatttatca aagaaaatta atgaaagtaa
    52321 atcagaaaat gttgaaatac taattgagag aaatggtgaa aaaataacta aaaacataaa
    52381 tttatcaaaa aatgcagatg gtgattataa aataggtctt tgggtaagag attctactgc
    52441 tggtgtaggt acacttactt tctatgataa agaaagtgga aaatatggag ccataggtca
    52501 tccaataaca gatagtgaaa cagaaaaaat tctttcaata aaaaatggag atcttttaaa
    52561 ttcttcaata ataagtataa aaaaaggtgt taaaggtaat ccaggagaat taagaggaat
    52621 tttttcaagt gataagacac ctataggaaa tgttacagga aatacacaat gtggaatatt
    52681 tggtagcatg aataaagaaa attttaaaaa tattaacaat aaaacttata aagttggttg
    52741 gagagatgaa attcagccag gaccagcaca aattataacc actattgatg aagacggtcc
    52801 taagctttat gatattgaaa ttgtaaaact tgcaaagcaa gatagtatta gtacaaagag
    52861 tatggtaatt aagattacag atgaaagact tttagaaaaa actggtggag ttgttcaagg
    52921 aatgagtgga agtccaatta tacaaaatga taaaataatt ggcgctgtaa cacatgtttt
    52981 ggttaataaa ccagaaattg gatatggaat ttatatagag tggatgttaa aagacgcaaa
    53041 aattatataa tagaaaataa gtgtgattaa tgtaaatatg tgttataata aatactagaa
    53101 aattaaatta attttttagt atttattttt tttagaagga aataaataag ttttgtcgaa
    53161 tatagttaga gtagaattat tgtaaaaaaa tggatatata aagttggtta aggagagaaa
    53221 agcatgaagg aatcaaaaat atctgtatta attgctgatg ataacaaaga gttttgcaat
    53281 attttaaatg actatttatt aagtcaaagg gatattgttg ttacaggaat tgcaaaagat
    53341 ggattggaag ctttaaaact tatagaagaa aaacaaccag atttagttgt attagatata
    53401 attatgccac atttagatgg attaggtgta cttgaaagaa taaattctct aggattagaa
    53461 aaaactccaa gagtaatagt attatctgct gttggacaag acaaaattac tcaaagagct
    53521 ataactttag gggcagatta ttatgttgtt aaaccatttg acatggatgt atttacagag
    53581 agaataagac aaatgtttaa tagcacaata tcagaggaac ctacaaatag taaacctaaa
    53641 attagtgtag ttgaaggttc aaaaagtgag gattcatttt ctgaggataa caaagagcct
    53701 atggatttag aggttgaaat aactagtata attcatgaaa taggagtacc agctcatata
    53761 aaaggatata tgtatttaag agaagctata actatggttg taaatgatat ggaattatta
    53821 tcagctataa caaaagagtt atatccttca atagcaaaga aatataatac tactgcaagt
    53881 agagttgaaa gggctataag acatgctata gaggttgctt ggggaagagg acaaatagag
    53941 gctataaaca aaatatttgg acatactatc cacaatgata aaggtaaacc aacaaactca
    54001 gagtttatag ctatggttgc agataaatta agactaaaga ataaagttag ctaaattaag
    54061 gagaaagcac tttttatcag ataatatttt cgctgataaa aagtgctttt ttatagagag
    54121 gtggaggtaa tttgagaaaa gcggtatatg tagaaggaaa tgaaaaaaag attattttag
    54181 ctaattgtat aactaaatct gagtatgaaa aaatatataa aaagaattta gtatgtccaa
    54241 tagatggatg taaggcaaaa cttggtttta gggaagttaa aaagcaaaga gatggaaaat
    54301 atttttatac attacctaat aataaacatg tttatggatg tccttataaa attcaaaaga
    54361 agaaaagaag agcaagaaga aaaatagata ataatttaag tgaggatttt tcacaaatat
    54421 cctttgaaat tttataatat ttatataaat ttaaaaataa ttttagattt tttaggtcaa
    54481 aaattgaggt gagatatatg gatttatatg aaaaaacttt aagtgaggag agcatattta
    54541 agtgttcttt tatggaactt gttaagcaaa aggtgaaatt accagatggc aatgaggcag
    54601 aaagagatat tgttaaacat tcaaaggggg tttgtgttat agctttcaat gaaaaaggaa
    54661 atattcttat ggtagaacag tttagaaagc cttttaatag agtattttta gaattaccag
    54721 caggaaaggt tgataaggaa gaaatattag aaaaagcagc cttacgagaa cttaaagaag
    54781 agacagggta tttagctaat aagataacat atttaggaca aatagcacca tcaccaggat
    54841 tttgtgatga agtggtttac ttatataaag ctcatgaact aaaaaaaggg aatacaaatt
    54901 ttgaccatga tgaattttta aatttaaagg aatatccatt agaagaagtc aaaaaaatga
    54961 ttatagaagg aaaaataact gatgcaaaaa ctatagcatg tctatttttc tatgaaaata
    55021 ttaaataatg agaaatagga actagagtat ttaagtctac tttagttctt atttttttaa
    55081 ataactttat tataaactta tctagtccta aatatttatt tttcagatag aactaagata
    55141 acatcttaat aagcagttag tatttcataa tatatggagt aataatagcg ttaagaagct
    55201 gagactgttt gaacgaaggg agtttcgaag ctttagctat tattacggaa tagatgatag
    55261 aagtactttg cgaagttaag tagttctcgg agtttagcta aaaaataaat atttaataga
    55321 gacttaaaaa taaatttaaa aaagtatgta tctttttatt atgttaatca tatcttttaa
    55381 taaatagctt taatggaggg gaaaaggaat ttatgaggat gttttttagg ggacttatgg
    55441 agtcattaaa ggaaaataaa atcacttttt tatgggtact tttattcttt ttagtcggaa
    55501 tagttttagg atcttatact gtttattaca tgagtgaatt taataggggt gaaattacta
    55561 cttattttaa taacttttta gagtttttag gtggaaactc tgtatcttat acaagtattc
    55621 ttgtagatag tataaagagt attttaccaa ttgttgtaat aatagtttta ttaggttaca
    55681 cagctgtagg aacaccaata attcttatga tagatttagc taaaggatat gttattggat
    55741 ttacattttc tttaatagtt agcatgttag gaagtaaggg agttatttta gtcttatcag
    55801 gattaatgat tcaaaatttg atttttattc caataataat gttaattagc gttttggcta
    55861 taagatattc atttacaaaa ttaaaaatca aagcatctag ggaaagaata aattttgatg
    55921 ttagtagaaa atatttaaat tttcaaggga ttttaagttt aattatgata tcaggaatac
    55981 taattgaaac atatatatca cctaatttaa taagattagt aattagtagg tgatttctgt
    56041 aatataaata attatgtaat aacattttgg ttaggagtta atatgaaaga tttagtaagc
    56101 aaatacgagg tatatctaga atcaataggg aagagaaaga ataccgtaag tgcatacatt
    56161 acagatgtct ctatgtattt agattttgta tatgaaaaag ggcttaaggt aagtgaggat
    56221 tcatatcctt taatatctta tgtacagcat ctaaaggaaa gccataaatc tccatcatca
    56281 attcaaagaa caattatttc tttaagaaat ttttataatt tcttagtagc agaagaattt
    56341 ttaaagaagg ttccaaattt gactatgaaa aaagaacgaa ttgaaaagaa gaagccttta
    56401 gttttaactg ttgaagagat aaataaaata atgaattcta ctaatgtttt aactgaaaag
    56461 ggaatacggg ataaggcttt attagaactt atgtatgcca ctggaatgaa agtttcagag
    56521 cttatatctt taagaattga agatattaat ttagattcat cttttgtaaa ctgtagagat
    56581 agtagagggt atgagagact tataccaata ggaaaggaag ctaaggaatc tattaaaaga
    56641 tatattgtgg taagagaaga taattctaaa ggatcagata aacttttctt aaatatgagt
    56701 ggagaaccta taactagaca gggagtttgg agaattgtaa aggaatatgc tgttattgca
    56761 aatattggga aagatataaa tttaaataca tttagacatt cttttgcagt acatttatta
    56821 caaaatggag caaatgctaa ggtggtacaa gaactattag gaaatcaagt tatgacttat
    56881 atagatatgt actatgatat tataaataat gaaaaaatta acaatatata cagaaaagct
    56941 catccaagag cataatatat aaaagaaaag gctttcatag aagtcttttc tttttttata
    57001 ttagaaagtt taacaaaata taaagataat ttgaactttg taattaagaa aagaaaaatt
    57061 tattaatatt gaaaaaatat aatattttat agggtgaaag tacttgtaaa taaggaatct
    57121 ctctgataaa ataaatttat gaaaaggtta tcacaaattg atagtgtgga ggaaagaaac
    57181 ttatgagaat gtatgattta atattaaaaa agagaaatgg tggggaatta agcactgaag
    57241 aaataaactt ttttgttgat aaatatacaa atggtgaaat acctgattat caagtagcag
    57301 ccttattaat ggctatatat ttccaaaaaa tgaataagag agaaacttcg gatttaacta
    57361 tggctatggt taattcagga gatatattag atttaagtga aatacacggt ataaaagtag
    57421 acaagcattc aactggtggg gttggtgata ctactacttt agttttggga cctatggttt
    57481 cagctttagg aattcctgta gctaaaatgt caggaagagg tcttggacat actggtggaa
    57541 caatagataa attagaaagt tttgatggat tctcagtaga aatgactaag gatcaattta
    57601 taaataatgt caataatata aaattagcag ttggtggaca aacaggagat ttagctccag
    57661 ctgataaaaa actttatgct ttaagagatg ttactggaac tgttgataat gtatcattaa
    57721 tagcttcaag tattatgagt aaaaaaattg ctgcaggcgc tgacgccata gtattagacg
    57781 ttaaggttgg agatggagca tttatgaaaa ctccagaggc agctagagaa cttgcaactg
    57841 aaatggttgg aataggtaaa catgttggaa gaaatactgt tgccataatt tcagatatgg
    57901 atcaaccttt aggatttgct ataggtaatg ctttagaagt taaagaagcc atagaaactt
    57961 taagaggaaa tggtcctaag gatcttttag agctttgttt aacacttgga agcaacatgg
    58021 ttgtcttagc aggtgctgct aaagatactg atgaagcaag aaaaatgtta atggaaacaa
    58081 taacttcagg aaaagcaatt gaaaaattaa aagaatttgt aaaggctcaa ggtggagatg
    58141 catcagttat tgataatata tcaaatttcc ataatgctaa atatgtaatt ccagttaaag
    58201 ctaataaatc aggggttgta agtaaaatac atgctgaaaa cataggatta gttgctatgg
    58261 aacttggagc aggaagagct actaaagaaa gtattataga tttagctgtt ggtattgttc
    58321 ttcaaaagaa aagaggagac aaagttaatg acggagatat aatagcatat attcatgcta
    58381 atgatgaaga aaaaggtaat aaagcaatag atggaatatt agctaattac gaaatatcag
    58441 attcagtaaa agatattcca ttaatatatg atatagttaa atagttttaa aaaatgattt
    58501 aaagctggtt ggattcttgt caagtatagc taagatttat agttatgcat cgtaatcttt
    58561 gaataaccat agctaataag taacccaatc actttaaata aattaaattt tattactcat
    58621 tatagatttt cttgttaaag ttatcctata cgaattaaag tataggataa ctttttcttt
    58681 tttgcttata atttatttct tttgggagaa aataagcaag aaaggaggag atattatgaa
    58741 aaaatacata agaaaaatag cagctttttc aatattttta tctttattta gcattgtttc
    58801 ttctgttcct gtaaaagctg aagaggcaat agaagtagaa gcaagatcag ctcttcttat
    58861 ggaagctagt acaggaaaaa taatttatga gaaaaatgct aatgaaaaat ttgcacctgc
    58921 ttcagtaaca aaggttatga caatgttgct tgctatggaa aaagtagata gaggagaaat
    58981 aaaactttca gatcaaatca cagtaagtga gactgctaag aaaatgggtg gtagtactat
    59041 gctcctagat gttggagaag taagaactgt tgaggagatt ataaaaggta taggaatagc
    59101 ttcaggtaat gatgccgctg tagctatggc tgaatattta ggtggaagtg aaagtggttt
    59161 tgttgagatg atgaataaaa gagctgagga gcttggaatg aaaaacacta catttaaaaa
    59221 ctgcacagga cttcctatag aaggacattt atctactgca tatgatattt ctattatgtc
    59281 tagggaatta ttaaaacatc ctactatatt aaaatataca ggaacttata tggaaactat
    59341 atcagaggga agaaaatctc ctattgaact tgttaaccat aataaattag ttagattctt
    59401 taagggatgt gatggtctta aaactggatt tactaatgac gctaaatact gtatatcagc
    59461 tacagcaact agggacggag ttagaatgct ttcagtaata atgggagctc caacatttaa
    59521 aataagaaat agagatgcta gtatgcttat gaattatgga ttttctaagt ttgaaagcaa
    59581 gcaagtagct acaaaagatg gtgaaataga acaaattcct atgaatcaaa caggagataa
    59641 gtttatcata ggaaaagcta aggatgattt aaccttagtt tgtccaaagg gtatgagtga
    59701 caaaatagag aaaaaagttg ttttagatga aagtaagaaa aaaattaaag aatttgatac
    59761 tattggatat tgtgaatatt atctagatgg tgaacttcta ggtaaagttg aattatattc
    59821 agatagagat atgaaaaaag gcggaattat tgatagtgtt aaattcaagg ttaagaattt
    59881 ctttaatggt gaagaggaag ataaagtaga agaaaaaaca gaagaaaaga gtaatgaaaa
    59941 ataaagtatt aactagacat tttgactgta ttcattaaaa actaaaaata aatacctata
    60001 agttgaaaga agaacaacta gcatatagtc tccttatgct agttgttttt gtatataaat
    60061 ttttcttata tagattcaaa gttagtaaaa taataactag agagaaggtt atttttataa
    60121 aatcaacttc ttgtatgcct taaacttagt gttaattgct tttttatatg atagttgtta
    60181 tgatattatg tgaagttttg aatagtagga gaaaaataga ctatggaaat gccaattata
    60241 aaacttaaaa actttgatgg accttttgat ttgcttttgc atttaatcaa aaaaaatgaa
    60301 atgagtatta ctgagataaa gattcatgaa atcacaaaac agtatttaga atacatagcc
    60361 ttaatgaagg aacttgattt agaaataact tcagagttta tagttatggc agctacttta
    60421 atagaaataa aatcgaaatc attacttcct aaggttaaag ttgaagatga gacttgtgag
    60481 gaagatcttc agaaaatatt aatggaaaaa cttcaagagt ataaaaagtt taagaaaata
    60541 agtgcttatt taagagaaag agaactttct actggagaag tatttacaaa aaaagctgaa
    60601 attattgaag ttgaagtgga caacaaactt gatgatgatt actttaaaaa tataactatg
    60661 cttgatcttt ataagctcta taataatctt atgagaattt acggtgaaaa acaaaatgta
    60721 aatgtcatgg agaaaaagat aagtgtagat aaatataaaa ttactgataa aattaacttc
    60781 ttaagggata agctaagtga aaagagcgtt gttagatttt cagagtttat accacagtgc
    60841 gaatgtaagc tagaagtagt tgttactttt atggcaatgc ttgaacttat aaaaagaagt
    60901 gaaattaaag ttgtccaata tgaaaacttt ggtgagatta tgatggaaaa ggtgatagta
    60961 aatgagtgat ataaatcaaa tagaatttag tgaaatatct aaaaaagacg agcttaagtc
    61021 tattatagaa tctcttttat ttgttagtgg agaacctctt gctttaaagg atatatgtag
    61081 aatagtagaa gaggatttta aatatgtaga agatttaatg agagaattaa tgaatatata
    61141 taatggagat agttcaagag ggataaaaat tattagttta aatggaactt atcagttagt
    61201 tactaaaaca aaaaatagtg aatatattca aaaactttta aaaaagaatg ttagacaatc
    61261 attatctcag gcttctttag aaagtttagc aataatatgt tataagcaac ctattactag
    61321 agttgagata gatgagataa gaggagtaaa aagcgaaagt gctatacaaa gacttgttga
    61381 aaaaaattta gttgaggaaa ctggtagatt agaggttcca ggaagaccta tactttatgg
    61441 aactacagat gaatttttaa gacattttgc attaaatgat ttaggagatc ttccatctat
    61501 agaattattt gaagaaaata atgaggtttc tgacatggtg gaagagtaga ttattctaaa
    61561 tttataaaaa gtaaaatatt ataacttaag gaatttagta taataatttg tttatataaa
    61621 ttaagaaggt agggaatatt aattattctc taccttctat tatttaaata gcaaattaac
    61681 tcattaaact ataaagtttc tatttcttac tccctgcgaa gtaagaattt tattttttag
    61741 gttttaggtt gttttattat ggagtttgat tggttgattt tattgtatta gagggtattt
    61801 ataatgttta actcttcaat aaaatcagga atagttaaga aactatttaa atgagttaat
    61861 aaagctatag caggctttta atcacaaaaa ttagaatctt cttttttgtc tttacacttt
    61921 ttattgccca tatccttaaa tatttcaatt atctgaggaa tattatcaac tattctgtca
    61981 taagtattag tttggtttac aggcaatagc cttacagaat cacctttaag aactaagaaa
    62041 gcaactggct ttacactaac accagcgcca gaacccccac caaaggcaaa tctatcatct
    62101 attttttcac attctttatt tggaagttct gagcctcctg aagcaaatcc aaaggaaact
    62161 tttgatattg gaattatgta acttccatct ctagtttcta tggcatctcc aactacagta
    62221 ttaacatcta tcatgtctct tatgttttcc attgttgtac ccattagatt ttctattggg
    62281 tgatttgagt ttatcatggt acaaacctcc taaagtattt ttaaactcta ttaaaaataa
    62341 tatttataac ttcttgaagt ttaataagta aagaaatcct atatatataa tttgagcaaa
    62401 atttatggtt attatacctt gaacttcaaa aaataagtaa ttttcattat taaatttagg
    62461 atttatgttt ccagtaaact tttttagttt aaaaaagtaa ttcaatcctg tgtatattaa
    62521 agataaaatt tgatgtataa atccatataa gatagcagtt aaagctgaat cttcacttga
    62581 aaaatttaaa tctaatacaa tttttaaaga gggtttaaat ttacttttag ttaataattt
    62641 tattaatttc ataatggtaa ggttagatgg aggttttaaa aaggttcttt tctttttaga
    62701 agatttcttt attttagtgt cattatttcc tttttttgaa a
//
LOCUS       DAEVGI010000005        52169 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEVGI010000005 DAEVGI010000000
VERSION     DAEVGI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 52169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52169
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.005"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            32..172
                     /locus_tag="K8M77_000283"
     CDS             32..172
                     /locus_tag="K8M77_000283"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084812.1"
                     /translation="MAKYLLIYKESLSGEMKLKKFNRMVNLAEYLQTNPEYIIIAIAE
                     EL"
     gene            275..451
                     /locus_tag="K8M77_000284"
     CDS             275..451
                     /locus_tag="K8M77_000284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084813.1"
                     /translation="MTKMDILMKQFERKSVQLAGAYMELEGCSVDEALQAVMEFVVKE
                     LNEELEWVGELLCK"
     gene            526..1032
                     /locus_tag="K8M77_000285"
     CDS             526..1032
                     /locus_tag="K8M77_000285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084814.1"
                     /translation="MIVKLHGETSLNKLPKIIKGIVSDVQARAGVPDAKFTIKDVEVG
                     ILFNVEGEKSYLSVTHDGVAEPFKVHVKLDKNGDIDTTVDNEKESFLDDYSRAVAKGE
                     QSPAGHDPIESIFNDDDLKEVHEEDGGDLVAKYYEHTKEDLLVVRYFRNGVLVGESGY
                     KKKLEKEV"
     gene            complement(1049..1498)
                     /locus_tag="K8M77_000286"
     CDS             complement(1049..1498)
                     /locus_tag="K8M77_000286"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7084815.1"
                     /translation="MKKVKLTAHVHSSANEGIISFLEICEEYGVQTRFKLADRLEERG
                     LTVRKCAELTGLRLGTISDLMNGKKSSVNFQHLWVLMLILGLTDINDLIEFHIPTEIK
                     DFFNIGTSAWIKTQEVPEITLIASQIINGNKAIVDEYERLLQKTFHK"
     gene            1634..2197
                     /locus_tag="K8M77_000287"
     CDS             1634..2197
                     /locus_tag="K8M77_000287"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084816.1"
                     /translation="MKIALFGKMRSGKDTVGKILVEEYGFKRFAFGDGIGKIIKEYFP
                     EAWAGGKPRKHYQFIGQQLRELNPDVWINYLIKTVWEEGRPYRIKGTEFNVVVTDGRQ
                     VNEAEKLKEAGYLIVKVTSPEKLRIERMKQLGDVFTAEQLNHETELQVDKVQADIEII
                     NDGTLEDLMVKVQKIISEHKGGIKCGY"
     gene            2187..2525
                     /locus_tag="K8M77_000288"
     CDS             2187..2525
                     /locus_tag="K8M77_000288"
                     /inference="COORDINATES: protein motif:HMM:NF016205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBI7084817.1"
                     /translation="MAISLDLPYTLNFMLGAMTNRAKLEADVYDSGIESANLIMDIDA
                     LIQESKLTKKQAEVVRLYYYKQMTQEEVSNVLRISQQAVLDHLNKIKDKLTKVIERWE
                     KLDKSKFNNK"
     gene            2551..2880
                     /locus_tag="K8M77_000289"
     CDS             2551..2880
                     /locus_tag="K8M77_000289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084818.1"
                     /translation="MQDTPPIEERKQIIEALNEEHFQEVGEQLPSYLLEMLGTWLLKE
                     TYSDKRTNKVAVEEFPILSESQILRRRRKVVQIECENALEILNYHLRNNSSTSKRNEE
                     TKGGRNF"
     gene            2880..3521
                     /locus_tag="K8M77_000290"
     CDS             2880..3521
                     /locus_tag="K8M77_000290"
                     /inference="COORDINATES: protein motif:HMM:NF019128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBI7084819.1"
                     /translation="MNISIKKFLFTGAVAFGIAVPLLMYNIQKNHNTQMLQVIEQQHK
                     QLEQQQQEIKALHKEIDKQKKEINAVNKELKGINNLVLDSVGYIPNKEEVQLLEKLVE
                     CEAGAEPYAGKVAVANVVFNRVKSKKFPNDIYSVIYQNNQFEPVVTGMIYNRTASADS
                     KKAVHEAMFGKKVVDPSILNFWADYLEPDNKLWQQVPVEFKIGTTCFGKEWGN"
     gene            3521..3709
                     /locus_tag="K8M77_000291"
     CDS             3521..3709
                     /locus_tag="K8M77_000291"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084820.1"
                     /translation="MNKKTNKKMKELLSFTIKGGYRPQKLEGEELQKYLIERSRGCGR
                     HKSKKDYRRREKHQKRLF"
     gene            3864..4421
                     /locus_tag="K8M77_000292"
     CDS             3864..4421
                     /locus_tag="K8M77_000292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084821.1"
                     /translation="MFKTLKKIRQKMIEASEKANAKADAIRNKATQKADKYIEEYLNS
                     EGKKRATAKACLMQLTLAYNRYTVDYIGGSPYFPHQQENLELMIIPQGVVFKNGLDFL
                     PFKDIKGISFKTHQQIEKDLTLTRMIAFGVYSLAMKKKRKVIHKYLIVECESEGMPYT
                     MAFAGSGVEGMYQDMFIRMKNLKTV"
     gene            4552..5745
                     /locus_tag="K8M77_000293"
     CDS             4552..5745
                     /locus_tag="K8M77_000293"
                     /inference="COORDINATES: protein motif:HMM:NF021759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HBI7084822.1"
                     /translation="MINKPVKQVNTKLVISELHRAFKLFNDELFKGNLPEPAIIIQSK
                     GNKKLTLGWCTVRKEWNDDFTQEDRYEINLVAEALNRGTLPVMATLLHEMVHLHNLVN
                     GIKDTSRGNTYHNMKFKKTAEEHGLKIEYAKKVGWSVILIQDKTLDLIKSANFDEAVF
                     SLGRKDFSVEDIEKKKSKSSSRKYVCPECGTIIRASKDVFVLCGICTNVEEGKIVPMI
                     KEHSKCEADSPTAEAPIKEPEAPAEEEKVETHKEVVEIVEVDDVLLDGDGNPIPVKQG
                     MINIKKEWTAEDIADILKETKEEAESLGLEAPPFDLPIEINPKMVAQFALYKEGKNIT
                     FSKKYLSEASDEEIIDTIKHQYLHHYEFMVNGLKVNHKKPFKSLCEQFGIETKVPLKN
                     HRAMK"
     gene            5924..6427
                     /locus_tag="K8M77_000294"
     CDS             5924..6427
                     /locus_tag="K8M77_000294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084823.1"
                     /translation="MTNLNTNKNFKGVEICLVNLGNTAQFNYYTINTMEDLKEAYMDM
                     KNRFLADEIEVQGISHSTELCENLWDILKASNEYELELERAIEIYEVVDCIEDFKKLL
                     KSESYYIIEADNELNAYIEFLEETGFLVGVPDHIINYIDYEALLRDFEFNNGSVKRLN
                     ESQYIIY"
     gene            6605..6757
                     /locus_tag="K8M77_000295"
     CDS             6605..6757
                     /locus_tag="K8M77_000295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084824.1"
                     /translation="MKKEYKGFTIILGRDCLGSESWVVYKDGEQLSDCLDCETEAIEW
                     IENYIG"
     gene            7129..7338
                     /locus_tag="K8M77_000296"
     CDS             7129..7338
                     /locus_tag="K8M77_000296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084825.1"
                     /translation="MSGTFIMVIKDKGQMFEEVHYCGNDEIQAQRRYKRLEGCQRLMV
                     RAEVKRQMIQNVPFIMSYEVIEVIK"
     gene            8186..8575
                     /locus_tag="K8M77_000297"
     CDS             8186..8575
                     /locus_tag="K8M77_000297"
                     /inference="COORDINATES: protein motif:HMM:NF024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBI7084826.1"
                     /translation="MNFENLTSAGLSASQKKCAELLVMKDINKMSMNQIAEEVGVNRS
                     TIFRWKQNKEFNDYLNALADEFQRSFLADAFGELRNILKYGKTHEKLKAVELILKNQG
                     KLKDNTEVNATVKQEVSVDEFLKQLGV"
     gene            9545..9967
                     /locus_tag="K8M77_000298"
     CDS             9545..9967
                     /locus_tag="K8M77_000298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084827.1"
                     /translation="MKVFCLTHNRMFRSYSSAVNDKSLKNLTISNIIKCCRGEIPSAG
                     KDLQGNKLQWCFFRDFINHKNFPCIESEKRSWVLCLTDGRLFRNNKEAGEFYNIDSVR
                     ISIQLSGKRPSAGFNEAGEKLVFMKYNDLVEAMKGESI"
     gene            9967..10332
                     /locus_tag="K8M77_000299"
     CDS             9967..10332
                     /locus_tag="K8M77_000299"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084828.1"
                     /translation="MKKVFAILSVILGIFIVVKTALWFNNVRYEEETVRAVVVNKEYV
                     PSRTYTTCIFTGKTVLPIVNHTSPKYNITVQWGSLTDKIDSKELYNATKVGDSINMKH
                     VKKYTKDTNEFLGDSIKQP"
     gene            10555..11436
                     /locus_tag="K8M77_000300"
     CDS             10555..11436
                     /locus_tag="K8M77_000300"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012464.1,HMM:NF014814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7084829.1"
                     /translation="MKDITKIFNRMEADMKKAGFSTEGIHTVKEALVTLAENITLGDS
                     EARKLFVKYGVYEAGYIKKELPMFFRLISDKVEEKKVKEQIKKGASKESIERQKKLEE
                     LGENRLIGYARVSTQDQNLARQFKALEEAGCQVIFSDKLSGKNVSRPEYKEMMETIKA
                     GDTVIVTELTRLARSTTDLFNMMAEFEKKGVSVKSLKESWLDTTTAHGKLMFTIMSGM
                     AQFERELMLERQKEGIKVAKENGVRFGVKLKEEADMDLAIQLAKEGNYTMTQIAKMCH
                     VSRTTLWRRLKDLGIRN"
     gene            11660..13252
                     /gene="terL"
                     /locus_tag="K8M77_000301"
     CDS             11660..13252
                     /gene="terL"
                     /locus_tag="K8M77_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBI7084830.1"
                     /translation="MSACSILMEKYREAEKHYRILRSEYDVLYFAYEYFSDERNPENE
                     NNLIPAGGKFENAPAFHIELCEKLDELRDNPTKKICWSVPRGHAKSGYMSNVFPVHQI
                     IFRKRHYILIVSETGGMSKSFVEWVGDQLKYNKKLREDFGEVLSPNKMQNEQDNQEGF
                     LTHTGIKVQASSIGSQLRGSRNGSYRPDLVILDDLESSKNTNTKELREKNLHWFNSVI
                     MPIGDITRTSFVYMGTLVHGQGLLPHVLQRSDFDGRIYSAIVSEPERRDLWEHIDKIL
                     HDVENPNREVEADNFYFEHKEEMDKGALTLWNERFTYYELMKIKVNVGSKAFASEYLN
                     KPSDDDSCIFKKEYFVFYDEHKIDLKKLDIYSFWDIAIGKSSRSDYNAILTIGRDRVT
                     GVIYVLDAWAEKIPMHKALDMAERKIRQWNPKIFGVETVQAQYDMYRQLRERLMKAGI
                     YSTRLLAFNPKGKKEDRIEQLEPLVEGGYLRFNKGHRLLLEQMELFPSHDHDDLPDAL
                     ASAIEIAGKQRKKTYYRKPKGF"
     gene            13315..14841
                     /locus_tag="K8M77_000302"
     CDS             13315..14841
                     /locus_tag="K8M77_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI7084831.1"
                     /translation="MINSNKLFEIGGLFPPQKHYERIKGYKENKKLFKGHHCEVFKNN
                     MLQGDRAKELLYISVNLAGIICKKSADFLFGEELSVLAGKGEHTPEQIALDRFMEENH
                     MNILLYENAITNAYAGDTFIKVRYGQEFAGELPPELDEPRVIIENVNPETVFPETVAW
                     DKSKIKTFHIAVPIYDEEANQWYLNVESHMSGKIKYCQYTITPIHYNSDQEPERFSID
                     GIVENSVVEVSTGVSVPLVVHIPNLSTSDTWEGIDDLTELHPLLDEINNRLSQIADIL
                     DKHSNPAMAVPSGLLDVDEDGNAQFRVAHDKVFEVMGKDDVVPQYITWNGQLVEAFKE
                     LDKLVDMVLVIAEIPAVALGKGDSGTSGSSGLAIKWRMNSLLAKVNRKKQYYSKGLKQ
                     VFYIAQKLEEVIGIADYKMTVPILHFQDGLPKDNMEEANIMSIRTGGAKTMSQKTAIM
                     QLNNMTEEQAEAEIKRIKEEEEANMEIADSGFFNNAEPTEDVVNDVLKGAKNPSEYVS
                     "
     gene            14922..15902
                     /locus_tag="K8M77_000303"
     CDS             14922..15902
                     /locus_tag="K8M77_000303"
                     /inference="COORDINATES: protein motif:HMM:NF017919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084832.1"
                     /translation="MTYQEYDEILKDILQNFNKNKPDIPSRVLEGSIDEITRTYGRAY
                     KRLFSELLEKLYTDFGVLANPSYQSQLGLLKMIELRMKELNSEVAVQVQKELERAYVS
                     AGAFHVLATENIKSIEELHGAVPYTELNTYKMEQIVKDTMEDLLFVTQHTSKELKKVV
                     RSTFSKNIQYHSLINESQKNVKRIIERDLSKKFLKESLQKNGFVGIVDSGGRKWNLKT
                     YVDMAVSTKMNQAYTEGLKDRAMVTGKDLAVIPEKGAKDSCSYFEGMLISLTGATEGY
                     MTYEQLQSTGLIFHPRCVHSPFPVGSIDLVHEDDIAFHEKKLKELKNFKK"
     gene            16089..16619
                     /locus_tag="K8M77_000304"
     CDS             16089..16619
                     /locus_tag="K8M77_000304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084833.1"
                     /translation="MEDKDIKTHEEKMEDEKEETKEPEDVKGTEEKKEDSKKVSEEAK
                     DDVEALKQELAESQAKVQEVETLTNEVTTLKNEIADKDAQIKEYETLVENMIEAKMNQ
                     VPADLKDLIPDNMDLKQKLAWLEKAESKGIFNKETKKKPTIEVGKPMNVETPTVDTSK
                     MTAGQLLKMAYSSVKK"
     gene            16747..17598
                     /locus_tag="K8M77_000305"
     CDS             16747..17598
                     /locus_tag="K8M77_000305"
                     /inference="COORDINATES: protein motif:HMM:NF016923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBI7084834.1"
                     /translation="MGMTLIEANKLTQDMLQKGVVETMVKTSGVLQRLPFIEVVGSGY
                     AYNLVTELPEVSYRAVNGQYKESTVGAEQMVEHLVILGGDADVDVYLTRTHSNINDLR
                     AMQTELKAKATARQFEVDFFKGTGASDTLKGLDARLAEGKVGTEIKSELKYEALNELL
                     DAVVDGADCLFMSKKMRRKLLTLLQKDGHYIENGSDAFGRPISMYGGVEIVPVEDSLI
                     PEGKVYAVKFGTDQYVHGLSNGGVQVRDLGELDTKPCFRTRIEFYCGLATKHTKCFAV
                     LDTNTPS"
     gene            17781..18179
                     /locus_tag="K8M77_000306"
     CDS             17781..18179
                     /locus_tag="K8M77_000306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084835.1"
                     /translation="MDTVKQVNDFIADNILHSEVWDSATEQQKKKAVNQANRTLKRMF
                     PKVYTAEVPIEHLAEQTLWILKIDDMFQRAELGATYIAVDGVAMNIANKDRSICPFLM
                     EVLNLSEGWNSRRKVGRYSTYMKDSFRKGW"
     gene            18181..18498
                     /locus_tag="K8M77_000307"
     CDS             18181..18498
                     /locus_tag="K8M77_000307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084836.1"
                     /translation="MGLKYILDNEQIFHIKNVGVDAWGINSSSLEKTAYSCCIRGAED
                     STPIEAQGGKQVIPSYTVSFNSKVPFTVGETLEVEGNRLVILRKKEVKDLSRKVMFTK
                     VTL"
     gene            18502..18960
                     /locus_tag="K8M77_000308"
     CDS             18502..18960
                     /locus_tag="K8M77_000308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084837.1"
                     /translation="MWYLIKSVVEFPKGIFEIDSNMINAVNKGMNEITDDLLRVAQQR
                     SPVDEGTLEQSGTSKVISSKRMIKGLVSFSARNRGYNYALKMDKGKYNLGKKSLSKSS
                     RGVRSKFSKVSMKVGSGYLSDSAEKCKDGYTKHINEELGKAIMKSGFKLK"
     gene            18971..19345
                     /locus_tag="K8M77_000309"
     CDS             18971..19345
                     /locus_tag="K8M77_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084838.1"
                     /translation="MSIVSLAEFMSALVGHQVYAINFPNYKKGDFLKLEITSGVMELG
                     GVDDFNVQFMARASHPAKAEQMCLDVLDKLDKLTNKEFNDGKCQLILAQAEAPQPFYV
                     GETEDGAFLMSVNFRLLTTTLT"
     gene            19360..19791
                     /locus_tag="K8M77_000310"
     CDS             19360..19791
                     /locus_tag="K8M77_000310"
                     /inference="COORDINATES: protein motif:HMM:NF017961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HBI7084839.1"
                     /translation="MAGKKVAGVDVLLKVKGSDGQFLAVGGQKGASLKRQAETIDVSD
                     KTSGGWSESIMGLKSWSVECDGFVTLGDAGFKLVHEAFDNRIPLEVEINVGDEGGYSY
                     TGRVVITDFPEEYASDDAVTYSLSLQGASPLVRKDNGATSH"
     gene            19902..20180
                     /locus_tag="K8M77_000311"
     CDS             19902..20180
                     /locus_tag="K8M77_000311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084840.1"
                     /translation="MAKERKMVTINLDKERHLYFTLNSLELIEDITGTSIDKIGETMN
                     MKTLKILLYAGLVHEDKDLTIDSVGDMIGIEKIEEVSKAIGEAFKVLK"
     gene            20291..20533
                     /locus_tag="K8M77_000312"
     CDS             20291..20533
                     /locus_tag="K8M77_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084841.1"
                     /translation="MAHIRAEQEAIDSQMQMLAWQTALLMNSTGNFKKKIKPTDLYTP
                     LAEIDVKQKVEYKPEDKKALQEELLSAFAGSDIVTQ"
     gene            20607..23696
                     /locus_tag="K8M77_000313"
     CDS             20607..23696
                     /locus_tag="K8M77_000313"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI7084842.1"
                     /translation="MASNEVLIKLVADVSSLQAGMKKAQQELSNLKNTAESSTSGIGK
                     ALGTVGKIIAGAFAVNTLKNFGADVIRTTATFNDSMSKVKALTGATGKQFDQMTALAK
                     KMGSETAHSSSAVAEAMSYMGLAGWNANQIMSALPSVLSLASAGQVDLAFASDMVTDT
                     MSMFSLKAEEATRASDVFAKVQAKSNTSINQLGESMKYVGATANAFGLDIETTSALLG
                     VMANNGIKGSMSGTSLKTILSRLSAPTKEVSEGFDMLNISLTDSSGHMKPLTELLPEI
                     KKKMAGLSNEQQIAVAKNIAGTEAMSGFLAIVNASTDDLPKLIEELRNSSGFAEKTAK
                     TMEDNLGGAIRGLKSSLEGVKLALGDAISPILIPIIQGMANALRLIPPTVTNIITFVT
                     PAFKGLGDIIKSCTNVVKDCVNWFKEHKLVAQALTGIVIGLVGAYATFKTALAVTNGL
                     KTMTDLLDTASIKMLLFQDRIAGASLSMTLASAKTVLWNTVSAIGTTVTTAFGTALSL
                     LTSPITLVIGAIVGLVAISYLLIKNWDTVKAFLSTCWEAIKTFAINTWNSMCSGISSI
                     MASIKGAVSNVWNSICTWISGVMTKISAVVSSVWNSIKASISTVLNVISSIISTVWHG
                     IQIGIAFVLNLILAVIVSIWNLVVTAISIPLNIIYNIVMMIWNQIKDTVMGVLNAILD
                     VVSAVWVAIKDKIMGVLNAILGTVTTVWNSIKEAVSTVVNVILNIVTTVWNSIHNTIS
                     AVLAVIVNVVTTYFNVYKSIIMSVLNIIKNIITTVWNSIKAVINTVLNIIEEIITTIF
                     NAVKNYITAVWNNIKAIITSVLSAIKSIVSSVWNSIKAVISTVLNAIKSVVSSVWNSI
                     KAVISSVGSGIKSTVSGVFNSIKSIVSEKMNTLKGIVSGAWNRIKSIFSAVLKPNIKL
                     PHLSISGKFSLSPPEVPHLGVSWYATGGIATAPSIVGIGEAGDEAILPLSNKHRMKPF
                     AHAVASMMPDNVKNSSDGGNVNINVSQLVVREEADIQRIAEQLYKLQERNKRARGKY"
     gene            23696..24448
                     /locus_tag="K8M77_000314"
     CDS             23696..24448
                     /locus_tag="K8M77_000314"
                     /inference="COORDINATES: protein motif:HMM:NF017520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBI7084843.1"
                     /translation="MMKFMVTFNGVDMPDCLKVKAVNTSILPEVKTSYKDVAGGFGVI
                     DTGTTIGGKTISLDVVIVPPAGKSLLEMQREVAFWLMGNDFKLSPLVISEECDLEYMA
                     KVNGAVNIKDLFFVGEGTIDFYVPKGIAVGRFTRYGANESGNKIIVDYLGTAPSYPIF
                     EFTPTKDYKNTTLRIAHIETGDTTLITGNFNAGEKIIIDSTKRLVKKGGVLSLDMIDL
                     KSKWLKLKGRRINTFTYNLEGEMVLSYQESWL"
     gene            24464..27037
                     /locus_tag="K8M77_000315"
     CDS             24464..27037
                     /locus_tag="K8M77_000315"
                     /inference="COORDINATES: protein motif:HMM:NF018329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI7084844.1"
                     /translation="MDLYILNRQEKIVGVLSNEGHNNFLSSAVMKESLREMDTLTLEV
                     SSLGIEVSEVKEENYVLFQDITGTWKNFIIREVNEIHGTDAMKQVYAEDSSQELIDYL
                     FFEELKGQKLSPSSLLGIILQGTRWEVGEVDKISPVTFPEETRKKTVLECIHLIVETF
                     NLQLRFRIEVAGNKIIGRYVDIKKHIGANYGKRFEYSKDIEKIERKVMSTRLKTAIIP
                     FGAVPEEEEKEESKLKDNIQNIEENQEEKPNKPPIDITGVQWTKPKNPLNKPFGQNYL
                     EIPEATAQWGYMDKEGKMKPRFLLYENTECKTPEELIQKAYEVLRDISKPVTNYKLEV
                     IDLFALTKDTELSFETVRLGDIVSVIDHEFTPAIQIKTSIIEREVDLLLPENTKIELG
                     SFIRNLVDSESSQQIQDMINASLSTGLEDIEAKVVDVSNTFEDFKKNYTSNELNLNWI
                     KNSDFSNGLKYYDSWNDSDFRLEAVNEIPYFDNCGRLSKGNFTQEIEGAEKLKGNNVT
                     FSAFVWGAGHLSLEITYINKEGHLIQDVLNSKQIPDHKGWNRYSMTVAITQQHVRKIL
                     VSFVPESDSYVTGLQLNLGAVPSKYSKNPSDKYGKGVYDQIRAVSDAEFKNGLGYVYL
                     EEEDGLWVYDKPSNNQPTKMTALKGGMLGIGNWNPQTRQWDIRTFIDGNMVNASCINT
                     GKLNADLIKTGRISSLDGSVQINMENGRFSFGVGGSGNTAQLTNDEFKIHYSDGSYTQ
                     MNKDGFRWWKAGNSRAYHCLATTIEFTASGDPNNDITVTLPTEFRGKDFTAQAVLSDT
                     YTDSWDWGQPWVVQRMVVLVSGINKENGTVTIKGYRTDKNYQTGAYRRCAIRGVLIVI
                     A"
     gene            27072..27320
                     /locus_tag="K8M77_000316"
     CDS             27072..27320
                     /locus_tag="K8M77_000316"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084845.1"
                     /translation="MDIKNFEKTMTVFFSKSTGKIKALCQGVQGFNFFGDEAVDMELI
                     LDRLVTDYNPKVMSNMDFYVVKEGKIEVNASVLEQFKL"
     gene            27336..29897
                     /locus_tag="K8M77_000317"
     CDS             27336..29897
                     /locus_tag="K8M77_000317"
                     /inference="COORDINATES: protein motif:HMM:NF022117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBI7084846.1"
                     /translation="MAIKSFSFKVDFITKNSLCMAHVKQNDNAIFNIYLTQQGQALDL
                     TGQQIRLFVRKPDGKLVVQETGITVVDAKGGAIRINVQNSIFQAVGVAVCEIDLWGND
                     NKNASSGSFLINIDEQLGSNDVVKSYVDVNLFRQLTEYIDEANAEIIKYKTLFEAFVK
                     AGVSLQGLNDIKAYIDNNLAELKFETDEAVDLIPKVNKARTDAEAKRVEVVNATNSAE
                     AKRVQVVDATSKAEAKRIEVVNVTATADNSKNTLIKATTNADNKKTELEGATASAEAK
                     RKEVVSVTNTANTSKSQLEIATANADKKKDEVVSVTANAEKKRAEVVGTTNTANEIKK
                     SLDGSIASGNSVKAGLDGSINTGNNLKAGLDASIKSGNDIKTALDSATSSANTKRVEV
                     ETATNNAEQKRIQVVAVTDSAEAKRKELDRLITQINAEGIMVKGNIPASRDCNDFKDF
                     NRWIAWDTGAGDFKNTPEGTLTKGTARVFVITNRGYSPQRIQQEFINVYPQNRVTRFV
                     RNYNEAGWGAWYKVYDEANKPTPSEIGAVNKAGDTITGYLKLNGFENSASYVRLAGGS
                     ENWLGAGTGATDVYICNDKSQKYLQLKNDGRLCYADNEVYHQGRKPTPSEIGALGKTE
                     KAVDSDKLHGMTPYDGKNGSGIPVVAKDGVMEVGKFLDFHAGGTGDYDVRLEAKAGEL
                     NVWGNFSSNDLWARKYLHVADWYNPSSGNAQFWYKNENRTLYLENAQELMINNSRVHH
                     DPQASPLWSGGWYVTDTQYVYPTKKLSQCNNGWVLVWSDFDPDTNKQNDTSWVCTYIH
                     KNCWHSGETDVLIPTNAFDAPIVKALQFQDHCFNGYGSNSTGEKADVILRFVYEW"
     gene            29924..30304
                     /locus_tag="K8M77_000318"
     CDS             29924..30304
                     /locus_tag="K8M77_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084847.1"
                     /translation="MIMLEEKIVQGVADASPVFTKYYVQINADNVVIGYSSTQTPEAT
                     NEIEVEADKLTEQFMKMPFFYRYDETVNDFIFDDSLRSAEAERRHNRLTKEQQLGQHI
                     SDLEIQLMMVQMMMQKQPQAPATV"
     gene            30324..30473
                     /locus_tag="K8M77_000319"
     CDS             30324..30473
                     /locus_tag="K8M77_000319"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084848.1"
                     /translation="MADNYVPSGLVKDWRFRFVNKWATDFHINMLVSLGVVTDRDAEY
                     IRTGV"
     gene            30519..30956
                     /locus_tag="K8M77_000320"
     CDS             30519..30956
                     /locus_tag="K8M77_000320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI7084849.1"
                     /translation="MLSMDYDKLLLGGIGILTTLIGGWDLTLQVLLILMATDVVTGVI
                     KGLYKWDFTSRAFRQGLMSKAGFLIVIILCYQVDTLLGNAEPIIRTTCAIFYITIEGS
                     SIIENLGEMGVPIPKALTKRLAKLKDMSDEENEESDQKGKIER"
     gene            31053..31790
                     /locus_tag="K8M77_000321"
     CDS             31053..31790
                     /locus_tag="K8M77_000321"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013670.1,HMM:NF019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7084850.1"
                     /translation="MRIGIDKGHAIKGVRGASAILDEVNENRKIGNRLIEMLKEKGHT
                     VVDCSCDVAYDVNEQLAGIVSRANRQYLDLFVSIHLNSGGGHGTETYTLPNPNESTKA
                     KAKAINDAVASSCGFSNRGLKTARFYVLRKTIAPAILVEVCFTDSQEDANKINVEAVA
                     KALFKGITGTEYDGSSDSNPSAPAGYGIVTASVLNVRKGAGTQYPVIGQLKKGDRVKL
                     DVKVGQWWSTYYGEHGGFVHGNYILVN"
     gene            complement(31920..33047)
                     /locus_tag="K8M77_000322"
     CDS             complement(31920..33047)
                     /locus_tag="K8M77_000322"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBI7084851.1"
                     /translation="MSDIKQGVLMQPNNLIKSEYDFTAIENRLFYKILYNAQKQHTKD
                     SLYKTVLTVEEIRTFMKKKADYTVESIEAKLDMFKTNILSFDYIEEGTGKVMTFNAGL
                     ITSHHYDHEEQLFIIEIHELIHKHITDFVKMQKEGYAPINLDLLFNFKGAYTQRLYTL
                     IRLWTRQNRKVDIKFTVKTLRSYLKALNKFSAYADFKKKVILPAIKEINTSGNMEIIF
                     ENKVDEIKKGRSVHELVFHVVDHETRKYFDNDAEAVKPKVIEVTEEESPEVKEKEKKA
                     LFRKFLLENTALSPYVLDSFIFEYGSFAEQFMWSDSPYLKMLLELQTKVLEKDGVSEI
                     GSRQIGYINKTLMTSIVEYDLEQDALRRCYQSPEYFDEYNW"
     gene            34076..34831
                     /locus_tag="K8M77_000323"
     CDS             34076..34831
                     /locus_tag="K8M77_000323"
                     /inference="COORDINATES: protein motif:HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7084852.1"
                     /translation="MVGSKNIKVELIKQYDVQTGTYHEERNIKVNMQSIQAGLIKTLG
                     ATPFAVLMAIVSFCDNEGEAFPSQRKLANITGLSLPTVHKAIKVLLETEIDGVPILSR
                     ELETLGSRTKFSVYTITPKKDASGKPKKKTSRDYVLKFKDLYEKTFGFNCVVNYGRDC
                     SLVKNKLMANFTEEEVDMIIEKGVLEYKDRWANSNYPYPSLTMVCTWIADILIKEHKQ
                     AQENAEEQQRIMEEAHQLAEVDDDRINKFFDSI"
     gene            35050..35952
                     /locus_tag="K8M77_000324"
     CDS             35050..35952
                     /locus_tag="K8M77_000324"
                     /inference="COORDINATES: protein motif:HMM:NF037161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBI7084853.1"
                     /translation="MKIYTGIDVGNGYCKYKGGRFASKVKHGSLSKVGAKRKNIHQIV
                     YKGKEYIVGDGGTFTGNKRYYTEEYEIALLTGIALASGGKVATIQANVVVGLPVDHYK
                     MNSEEVQKHLNNLGVKEITVDGKEYIIEFESVTVFIEGAYPIGYADDSHMITIDIGAG
                     TINVIEWENQEIINKFTFDQAFYKMHKDLAEFLNENYGIGITPNDAEKYVGKSIIDTE
                     EGEVEVPEIDEIVGSFISNIATTIENSFHTKTVKKIKIFGGGAKDTFQHWKKKFAKAE
                     LVKESQHVNQAVYQAVAESMAEEE"
     gene            35952..36245
                     /locus_tag="K8M77_000325"
     CDS             35952..36245
                     /locus_tag="K8M77_000325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084854.1"
                     /translation="MKKRKPRKDGRYSINISFTEQEIDLIEHVDERGNFSAYVKYLIR
                     KDMEGSGANALDLIQGLLKDNIQNKKQEQPPEEPQKPKANKSKINKILNKSNL"
     gene            36368..36847
                     /locus_tag="K8M77_000326"
     CDS             36368..36847
                     /locus_tag="K8M77_000326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084855.1"
                     /translation="MKNTKMMKILNEEMAKFDDVSKLEEVKETVTLTMNDAVEEIKNS
                     AQEFASTMSLTEMGLIEVTDEVRMSVIATMIKGAIAGAVVEEITNQLKEIDSVIEFNK
                     EFLEESKVTGFAEKETKDEDEAKVENEDVFEKLISELIIGALIEGIENIKKEVDGEA"
     gene            36877..37641
                     /locus_tag="K8M77_000327"
     CDS             36877..37641
                     /locus_tag="K8M77_000327"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084856.1"
                     /translation="MSFNLTVNTLEFWLKQPEFAPPKINMTRAETNDSNYKLMEANNR
                     ILGRSFHQTFSKWFKSYKRIPTPQEFIAMQMNDVKKNYNTPTWRKTHKVTFQWTPTVE
                     KGIKQRLLRSYISFINEIHTELMIQDLYTGIKIQRSDKLDYSGIDILAIDSKSKVTHE
                     IHITKNSPYAIDFLFKKEGKELCFKRLQNKLWAYPCWNKINHSIYSKRSFRGHVFLLY
                     DNHETYSTKIINGYPLFKKEYIMNKLDTNCYLKVGV"
     gene            37724..38491
                     /locus_tag="K8M77_000328"
     CDS             37724..38491
                     /locus_tag="K8M77_000328"
                     /inference="COORDINATES: protein motif:HMM:NF012234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI7084857.1"
                     /translation="MLSEKCMFSNTCKNKDTDLCNPLCYPFIVLHGQKGETGFWKASG
                     VPNKYKKVMFHNLPIKAENPKAYDVAERYINKIESVVEEKGIGLFLYSIPNTENTFGT
                     GTGKTTTAVAILNEYVLNATRRHLKGQKELKKNPALFIKASELQNKYNAQFRGTFEMQ
                     QEASNCFYRLKERMKGVSLLVIDDIAVRDITEAFKNELFEILDYRATESKATIFTSNY
                     PITKLVEFLGERIVSRIDGMTIQVGFQGKDHRKGGIF"
     gene            38491..39762
                     /locus_tag="K8M77_000329"
     CDS             38491..39762
                     /locus_tag="K8M77_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7084858.1"
                     /translation="MANIESKLLSKVVDEKDITVLGKYNITANDFTTQAETFNFIKRY
                     IKEFGEVPAYTEVVAECSSFEYVPEVPDNMAYMCKKIKSDNAKRRIFELLQKEASEKF
                     SSMTGTAFVEWLHKETSKIKEVACVDSHAGSNFATNGAERKAMYLEAKEIGSGLYIET
                     PYSKLTRWLNGGFELGDYVLLQAYTNRGKSWIASDIGICSWRSGNGVLHYSPELSQKQ
                     QLQRLDTLNGHFRNSEIKKGQLSGKAEEQYLKYLDSFNENNETPYIIKTMGDLPNGLS
                     VDVIEADLQANENIKMVIIDGFNLMNHKGNDGNRNNMTNTSRRLRQLFGKYKVVGVVV
                     HQVPTSAEKENRSEDETGARMVEPPRIDQYSETVAVIQDACTILNFDQADGVGKIRLA
                     KARTPHVDEILELQCDFDLGYIKEVSAIDFI"
     gene            39774..40001
                     /locus_tag="K8M77_000330"
     CDS             39774..40001
                     /locus_tag="K8M77_000330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084859.1"
                     /translation="MTREESNLLFKHLNRKKYIEAMEETQFGWKSAENFPNVCCPSEL
                     EFISVEEEPYKGECHTTCEQCWKYVLENKNW"
     gene            40216..40986
                     /locus_tag="K8M77_000331"
     CDS             40216..40986
                     /locus_tag="K8M77_000331"
                     /inference="COORDINATES: protein motif:HMM:NF015340.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HBI7084860.1"
                     /translation="MNRQLQIIDQREVLGKDFKIYGTKEEPLFLAKDVANWIEHRNIT
                     HMMKTVDEDEKLTYTISNSGQGREMWFLTEDGLYEVLMQSRKPIAKAFKKEVKQILKQ
                     IRMTGGYIPVKEEETAEQLMARALMVAQKTLEQKDLILKQQQEKLQEQKPKVEYYEEV
                     LDSSRLITVGQIANDLGITPQKLNQYLKLKGIQNNQYKTGWKISAKYNYLIEEGYADY
                     HIYTKHSVQAQQLKWTEKGRKFIIDMFNEDKRIFENRK"
     gene            41089..42072
                     /locus_tag="K8M77_000332"
     CDS             41089..42072
                     /locus_tag="K8M77_000332"
                     /inference="COORDINATES: protein motif:HMM:NF024554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBI7084861.1"
                     /translation="MKGLNEMEKLKVNGHLIDVDYPAELELYMDRLEKVRIRGEKVQA
                     CSPFRYEQHPSWAVNLDNGSWVDSGATKEGERKGNFVTLLAHFRGETYEETAEYLLEK
                     YAHFLDDAESLKLNLNLKLNEPEVTVLSQEKYADVVDKPSNYLLRRGISVKIQKYFQT
                     GRGSKGDCVAIPWHDKAGRIINIKYRSTQGKEFWFSKGGQPIKQHVYGLFAVLNLKVK
                     EVWAVESEIDALYLWSMGFPAIAFGGASMNDTQKKLLLNSGIETLVIATDNDVVGKRF
                     GQVLKEEFAGILNLKKIVFPVGVKDVNDLNPEELMNCANNLNIIKDFIKLV"
     gene            42541..43227
                     /locus_tag="K8M77_000333"
     CDS             42541..43227
                     /locus_tag="K8M77_000333"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084862.1"
                     /translation="MTKKYKYELSTIYEKVNEIVKEIQKESTSEVQKNFLIGDLLEVT
                     EELVQNNCKKFIAKYSIKDISQEELYSIAISLPLLDALKWFSFEKGNNFMATWNGFMQ
                     KRFLNELNELSNDKNKWFRNNVSSADKELSEDGTTIIDIVGEEDFTEEVCEEMELMNI
                     IKQFEEVDKHGAVIRCFFIDSQEYRTEAIKKALGVKEYGSKERKQVQRAKERFIKYLI
                     HNNYDVSKYL"
     gene            43349..44044
                     /locus_tag="K8M77_000334"
     CDS             43349..44044
                     /locus_tag="K8M77_000334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084863.1"
                     /translation="MKNNMFTARGQEAKASAEKGKVDLKKVYLRMKSGDSIKVRVMSP
                     ADYVEYLAHSSFTHKVYTQPCVGVIGQECALCKASKSGIEGFDALYAKKRYLFVFADM
                     TSGELRVLDVSKNQAKKLISDIEEYKEDLNSLAFNLKKVGEGTNTGYALSPIIRMKDT
                     DKAQFEACEGLEVTDEYLNTVLMPRTLELQVKTLKEAGFPTDEYFPHVDISDKEEGSE
                     DTGRIADEVLDNM"
     gene            44110..44922
                     /locus_tag="K8M77_000335"
     CDS             44110..44922
                     /locus_tag="K8M77_000335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084864.1"
                     /translation="MKYKFDIQEISKERALEMVTKFHYSNTLPTLNKIFLGFFLEGEL
                     VGMVTLGWGTRPLHTIKKLFPSLDTKDYFEIGRMCMTEDMPRNSESQMLSQMVKWLKV
                     NKPKLKVIFTWADGMLGKVGYVYQASNFLYAGQVESEIYLHNGVKIHPRQTKALFGLE
                     GDKRKTVRPSWEQMQEHGIKHYKGNQFRYLLFLCNKKEKKKLMKEALVDLSYNYPKED
                     ALSWKVKVGNRAWQKCPPPNYITDRKFNKNEDYLTEKGHKAFEKTEFIIRYI"
     gene            44940..45851
                     /locus_tag="K8M77_000336"
     CDS             44940..45851
                     /locus_tag="K8M77_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBI7084865.1"
                     /translation="MTQLFNRLGAERLKKRVIENDSERGQRLAGALYNQMNDLHSIDF
                     FPDYEIEKLLLEQKEHDLELIKNHQRLPEGLIVFSPSSASACRRGLVFKANKEKKDEV
                     LRYPYQRRWTRNSSAVHEAVQRDLLYCEKVLDNPMFTVHRLESGLPAWEQNIKTHKII
                     EHNGVKFFLNGMCDGILEYKPDGSKILFEFKTKSTTIGCVGTYKLKNVQEEHRIQAVA
                     YSLLFGVDECIFMYESVAKDGWTKGADAKIDFRTFYVKVTEEDKKELLDKFSEVAKDF
                     YNNKIPSKEDNKCFFCVYKEACERAGE"
     gene            45855..46139
                     /locus_tag="K8M77_000337"
     CDS             45855..46139
                     /locus_tag="K8M77_000337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084866.1"
                     /translation="MKRIYVQEGIYIVVNNSGTVPKKCFEVAKRRTFPTLPSAIKLFK
                     FLHRKYDSVYMYHTKAGFMVEFKTYAESLGIAQDIKNDIEATLEVFENDK"
     gene            46129..46644
                     /locus_tag="K8M77_000338"
     CDS             46129..46644
                     /locus_tag="K8M77_000338"
                     /inference="COORDINATES: protein motif:HMM:NF014166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBI7084867.1"
                     /translation="MISNKRILAMDLSLNLPAFCVAEVDEAGEFKVIEVSHIDNKKDK
                     KLETAEKLQKLYLHLKDIFQRYGEFDVVVREKGFSRFANTTQLLFRVVGVSDLVALQE
                     AGIKSVEEIPPTTIKSIIAGYSRADKEEVEEGVRTFLSKEQKNMKFYSDDESDSVAVA
                     LTYAIKKGWMK"
     gene            46884..47144
                     /locus_tag="K8M77_000339"
     CDS             46884..47144
                     /locus_tag="K8M77_000339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084868.1"
                     /translation="MRVIKKKKVDCYVGDIVLYKDYPCLIAEEEREANPYILICLEGI
                     KAGQVIGSYETLSRIDDVCSEILIKKDKVIISSEEGDESCPF"
     gene            47132..47713
                     /locus_tag="K8M77_000340"
     CDS             47132..47713
                     /locus_tag="K8M77_000340"
                     /inference="COORDINATES: protein motif:HMM:NF012487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI7084869.1"
                     /translation="MSILTVNVGGMFSGKSTELQRQGERHVLAGHKVLFIKPDMDTRY
                     SDCEVVTHKGQKVKAISVPTYADLKNYINTAEVDVVLIDEVQFFSERIIPSIWWLLSK
                     GIRVYASGLDMDFLSDGFPTTEKLMAMADVVQKFHAVCEGCGEDAVITAKRNFHEVGS
                     SDVIELGAKETYIPLCRSCYIKYMATKGEDSNG"
     gene            47706..47852
                     /locus_tag="K8M77_000341"
     CDS             47706..47852
                     /locus_tag="K8M77_000341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084870.1"
                     /translation="MVRLELKSIENYCIQQQKRIAELKVIIQNKDKEIERLTKLLKDK
                     ERDR"
     gene            47856..48506
                     /locus_tag="K8M77_000342"
     CDS             47856..48506
                     /locus_tag="K8M77_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBI7084871.1"
                     /translation="MVITVGAMIGAGKSSLAKLIGEHFGTDVFYESVEDNPILPLFYT
                     ASEEEIQEKRYPFLLQLYFLNTRFKSIKEALVHQNNVLDRSIYEDWYFAKVNMELGRI
                     SELEFKIYEELLENMLEELDELPKKAPDLMVYLSGSFETILERINKRGRDYELAPELV
                     DYYRTLWEGYDEWMFNHYKASEVLVIDIDKYDYVNNEEDAKEVLKMIEDKLAEIRG"
     gene            48566..48988
                     /locus_tag="K8M77_000343"
     CDS             48566..48988
                     /locus_tag="K8M77_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HBI7084872.1"
                     /translation="MYLASPFFNVVENERVDKVKEILDSKGLEVFSPKEHQNKHLEFG
                     TIEWRKATFKNDVDHIDWCDCVVAIICQGNYDDSGTAWELGYAYATNKPVILVNLTGE
                     AINLMIADSLHALITSYDELKDYDFNELKRIPYENYVW"
     gene            49017..49301
                     /locus_tag="K8M77_000344"
     CDS             49017..49301
                     /locus_tag="K8M77_000344"
                     /inference="COORDINATES: protein motif:HMM:NF016109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084873.1"
                     /translation="MELAVFFNLLQLIGGIILSIGYIPQIRKTIRTKSVEDLSSAYYV
                     NIFIGVGCMEAYAVYSALHGVAYMFLVTNTIAWLLGGTMLFLTLKYRKRV"
     gene            49305..49505
                     /locus_tag="K8M77_000345"
     CDS             49305..49505
                     /locus_tag="K8M77_000345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084874.1"
                     /translation="MKKILIFILVIILAIIICFAGLGLITFFLNNFLVAIGLNAVSII
                     NISSLLLAILLIKWFLKTERKN"
     gene            49615..51987
                     /locus_tag="K8M77_000346"
     CDS             49615..51987
                     /locus_tag="K8M77_000346"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012688.1,HMM:NF013755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBI7084875.1"
                     /translation="MQLNLNLDINNTKEAKAEARVKEAKKKKALAKYEPTWDEVWVTG
                     YKTHTGATKKAIFQTKLTDIDKEKLLAVKEAIESGELQTGVESLSKFTKTKALGLWVV
                     LKEIRRESIIKKMVEEKPENYHLILNPKELHELTNKLDKEEIIAVDTETTGVEVFGKD
                     YIVGISLTLPNADYHCYIPIRHVHEIEFIDEQGFTATKYVRIEEQLKDTLVFNVLKPF
                     LENPNLKKMFHNSKFDIHMFRKEGVVIKGLYLDTMVAMHLLNENEPSFALKNLATKYG
                     RYFGFEDKSMTYEELFGKGGFEKTPLDIGTVYACKDTHLTYKFGCWILEQFERLPKIK
                     KLYFEIELPITEVCIEMEKNGFAIDLGFAEQYKLELQEEIKGLEDRIREGFGEININS
                     NQQLQKVIYEEWGVPDDFKTKVDAPTLKQIVSRYKKDIPKVEYINDLLKYRELNKLLT
                     TYIEPLPQKISPIDGRLHGSFNQSATVTGRFASNNPNLQNLPYPARKMFVAPEGKLII
                     GSDFSQIEPRFLSHISQDADFMGAYLEGRDLYSEIASKTFKVPIEECCDGSIWRKKSK
                     VVLLGCMYGISAMSLAEQFNLTLAEAEQIYTDFYGAYPTMTAWFKATNDKANELGYVE
                     TTMGRKRRFLGHKDIARKYFAIHKNMQRKLKKESFNIWEEYRKKKVTYAEARAYQDVA
                     GQFNRVQRQSINAVIQGSSADIMKKAMVDIYRHLQKKGSEWKLLGTIHDEVLVEIPAT
                     ATIEEIQEIADIQRNAVKLSVPMKCDVAIMKRWGIDISLQEYAEGKEVPC"
BASE COUNT        19778 a         6619 c        10095 g        15677 t
ORIGIN      
        1 aaaaaaaaaa gagggggagg gtgattaagt aatggcaaaa tacttactta tttataagga
       61 atctttatca ggtgaaatga agctaaagaa gtttaacaga atggttaatt tagcagagta
      121 tcttcaaact aatcctgaat atataataat tgcaattgca gaagaattat aaaagatagc
      181 tgaaagtcta ttcttttttt tgagaagtca caaagcttat ttaatcatta atttaccata
      241 tgtaagcaaa ataaagaaag gaaagtgcta gagcatgaca aagatggata tattgatgaa
      301 gcaatttgaa agaaaaagtg ttcagcttgc tggggcatat atggagcttg aagggtgttc
      361 agtagatgaa gctctacaag ctgttatgga atttgtagta aaagaattaa atgaagaact
      421 tgaatgggtg ggtgaattat tatgtaaata aatttaaact taaaaaataa aatagattta
      481 ttaactgttg attttaagaa actttaaagc tggaggtatg ctgttatgat agtaaaatta
      541 catggtgaaa caagtctgaa taaattacca aagataataa aggggattgt ttctgatgtg
      601 caagcaaggg ctggagtacc tgatgcaaag tttacaataa aagatgttga agttggtatc
      661 ttatttaatg ttgaaggtga aaagagttat ttatcagtaa cacatgatgg ggtggctgaa
      721 ccattcaaag ttcatgttaa attagataaa aatggagata ttgacactac tgttgataat
      781 gaaaaagaaa gctttcttga tgattattca agggctgtag caaaaggtga acagtcacca
      841 gctggtcatg acccaataga atcaattttt aatgatgatg accttaaaga agtgcatgaa
      901 gaagatggtg gggacttagt tgccaaatac tatgaacata caaaagaaga tttacttgtt
      961 gtaagatatt tcaggaatgg ggttcttgtt ggtgaatcag gatataaaaa gaaattagaa
     1021 aaggaagtat aaaagcttcc tttttttttt atttgtgaaa tgttttttgt aaaagtcttt
     1081 catattcatc aacaattgct ttattaccat taatgatttg acttgcaatc aaagttattt
     1141 caggaacttc ttgggttttt atccaagctg atgttcctat gttgaagaaa tcttttattt
     1201 ctgtagggat atggaattca attaaatcat ttatatctgt aagtcctaag attaacatta
     1261 acacccaaag gtgctggaag tttacagaag atttttttcc attcattaaa tcagaaattg
     1321 tgccaagtct taatcctgtt aattcagcac attttctaac tgttagcccc ctttcttcta
     1381 acctatcagc aagcttaaat cttgtctgaa caccatattc ttcacaaatt tcaaggaagc
     1441 tgattatccc ttcatttgct gatgaatgaa catgtgctgt tagtttaact tttttcattt
     1501 ttttgattct cctttgttaa gtttgtttac acttccattg taccattttt gtgtaacaaa
     1561 gtcaatgata caccagtcac aaaccttata aaatacataa tttatattat gtaaaaagaa
     1621 aggagttgat tcaatgaaga ttgccctttt tggaaaaatg agaagtggga aagatactgt
     1681 tggcaaaatc ctagttgaag aatatggctt caaaaggttt gcttttggtg atggtatagg
     1741 gaagataatc aaagaatatt ttcctgaagc ttgggctggt ggaaagccta gaaaacatta
     1801 tcagttcata ggacaacaat taagggaact taatcctgat gtatggataa attacttaat
     1861 aaaaactgtg tgggaagaag gtagaccata cagaattaaa gggactgaat tcaatgttgt
     1921 tgtaactgat ggtagacaag tcaatgaagc tgaaaagctg aaagaagctg ggtatttaat
     1981 agtaaaagta acatcacctg aaaaattaag aattgaaaga atgaaacagc ttggtgatgt
     2041 attcacagct gaacaactta atcatgaaac tgaattacag gtggacaagg ttcaagctga
     2101 cattgagata ataaatgatg ggacacttga agaccttatg gtgaaggttc agaaaataat
     2161 ttcagaacat aaaggaggaa tcaagtgtgg ctattagttt agacttacca tatacattaa
     2221 attttatgct gggagcaatg accaataggg caaagcttga agcagatgtg tatgattcag
     2281 gaatagaatc agcaaatctt attatggaca ttgatgcttt aatacaggaa tcaaagctga
     2341 caaaaaaaca ggctgaagtt gtaagactgt attactacaa acaaatgaca caagaagaag
     2401 tttcaaatgt gcttaggata agtcaacagg ctgtacttga ccacctaaat aaaataaaag
     2461 ataaacttac aaaagtaatt gaaaggtggg aaaagcttga taaatcaaaa ttcaataata
     2521 agtaaatatc ataataaggt ggaaagtata atgcaagata ccccaccaat tgaagaaaga
     2581 aaacaaataa tagaagcatt aaatgaagaa catttccaag aagttgggga acagcttcct
     2641 tcttatcttc ttgaaatgct tgggacttgg ttattaaaag aaacttattc agataaaaga
     2701 acaaataaag tggctgttga agagtttcca atattatcag aatcacaaat attaagaaga
     2761 agaagaaaag ttgttcagat agaatgtgaa aatgcacttg aaatattaaa ttatcattta
     2821 agaaataata gttcaacttc caaaaggaat gaagaaacaa agggagggag gaacttttga
     2881 tgaatatatc aattaaaaaa ttcttattta ctggggctgt ggcatttgga attgcagtcc
     2941 ctttacttat gtataatatc caaaaaaatc ataatactca gatgcttcag gttatagaac
     3001 aacagcataa acagttggag caacaacaac aggaaattaa ggcattacat aaagaaatag
     3061 ataaacaaaa aaaagaaata aatgctgtaa ataaagaact taaagggatc aataacttgg
     3121 ttcttgatag tgttggatat attcctaaca aggaagaagt tcagcttctt gaaaagcttg
     3181 ttgaatgtga agctggggct gaaccttatg ctggaaaagt tgctgttgcc aatgtggttt
     3241 ttaatagggt aaaaagcaag aagttcccaa atgatattta tagtgttata tatcaaaata
     3301 accaatttga gcctgttgtt acaggaatga tttacaacag aacagcttca gctgattcaa
     3361 agaaggctgt tcatgaagct atgtttggaa agaaagttgt tgaccctagt atattaaatt
     3421 tttgggctga ttatttagag cctgacaata aattgtggca acaagttcct gttgaattta
     3481 aaattggaac aacatgtttt ggaaaggagt ggggtaacta atgaataaga aaactaataa
     3541 gaaaatgaaa gaattattaa gctttacaat aaaaggtggg tacagaccac agaaacttga
     3601 aggtgaagaa ctacaaaaat atttaataga aagatcaagg ggctgtggaa ggcataaatc
     3661 taaaaaggat tatagaagaa gggaaaaaca ccaaaaaagg ttattttaat tttgttacat
     3721 aaaaatgtaa cattttaaat tactatttta ggaagtccaa atctttgtaa atacagagat
     3781 ttggctatat ttttattgac attctaaaaa tatttttata taatattatt acagtattaa
     3841 ggaacaggaa gggaaggttt aaattgttta agacattaaa aaagataaga caaaaaatga
     3901 ttgaagcaag tgaaaaagca aatgcaaaag ctgatgcaat cagaaataaa gcaacccaaa
     3961 aagctgacaa atatattgag gaatatttaa attctgaagg aaagaaaaga gccacagcaa
     4021 aggcttgttt aatgcaatta actttggctt ataatagata tacagttgat tatattgggg
     4081 gaagccctta ttttccacac caacaagaga acttagaatt aatgataatc ccacaagggg
     4141 ttgtatttaa aaatgggctg gacttcctac catttaagga tataaaagga ataagtttta
     4201 aaacacacca acaaattgaa aaagatttga ctttaacaag aatgattgct tttggtgttt
     4261 atagtttggc tatgaagaag aaaagaaaag ttatacataa atacttaata gtagaatgtg
     4321 aaagtgaagg tatgccatat acaatggctt ttgctggaag tggtgtagaa ggtatgtatc
     4381 aagacatgtt tataagaatg aagaatttaa agactgttta atacagtctt tttttttatt
     4441 gtatttttat tgaaaaatat ctttcaaaat attgttgaca aagttgtttc aatttagtat
     4501 actcattctt gtaaagaata tcagaaaaca aattttgaaa ggaaggctaa aatgataaat
     4561 aaaccagtaa aacaagtaaa tacaaaatta gtaatttcag agcttcatag agcttttaaa
     4621 ttatttaatg atgaattatt caaaggaaat cttcctgaac cagccatcat tatacagtca
     4681 aagggaaata aaaagctaac acttggttgg tgtactgtta ggaaagagtg gaatgatgat
     4741 tttacacagg aagatagata tgaaattaac ttagtggctg aagctttaaa cagggggaca
     4801 cttcctgtta tggcaacact actacatgaa atggtacatc ttcacaactt agtaaatgga
     4861 ataaaagata cttcaagagg caatacatat cacaacatga agtttaaaaa aacagctgaa
     4921 gaacatgggc taaaaattga atatgctaaa aaagtaggct ggtcagttat tttaatacaa
     4981 gataaaacac ttgatcttat aaagtcagca aactttgatg aagctgtttt cagcttagga
     5041 agaaaggact tttcagttga ggacatagaa aagaaaaaga gcaaatcttc ttcaagaaaa
     5101 tatgtgtgtc ctgaatgtgg tacaattata agagcatcaa aagatgtgtt tgtactttgt
     5161 ggaatttgca ctaatgttga agaaggaaaa attgttccaa tgattaaaga acattctaaa
     5221 tgtgaagcag attccccaac agctgaagcc ccaattaaag aacctgaagc accagctgaa
     5281 gaagaaaaag ttgaaaccca taaagaagtt gttgaaatag ttgaggttga tgatgtttta
     5341 cttgatggtg atggtaaccc tattccagtt aaacaaggta tgataaatat aaaaaaagaa
     5401 tggacagctg aagatatagc tgatatttta aaggaaacaa aagaagaagc tgaatcttta
     5461 ggattagaag ccccaccttt tgacctacca attgaaataa atccaaaaat ggtggcacaa
     5521 tttgcccttt ataaagaagg taagaatatt acattctcaa agaaatatct ttctgaagct
     5581 tctgatgaag aaataattga cactataaaa catcaatatt tacaccacta tgaatttatg
     5641 gtgaatggct taaaagtaaa ccataagaag ccatttaaat ctttatgtga acagtttgga
     5701 attgaaacaa aagttccttt gaaaaaccac agagcaatga aataaaaagg ggagggcttc
     5761 agcttcttgg ctgaagttct atcctaaaaa tattttaaaa gtttttatat tttttttata
     5821 aaaaatgttg caattattgt ttcaatataa tataataata actgtagtaa ggaactacaa
     5881 aaatacttaa cagaaaatac ttaacagaaa gaaggaatta atcatgacaa acttaaacac
     5941 aaacaaaaat tttaaagggg ttgaaatatg ccttgtaaac ttaggcaaca cagcacaatt
     6001 taattattat actataaaca ctatggagga cttaaaagaa gcctatatgg acatgaaaaa
     6061 tagattctta gctgatgaaa ttgaagtaca aggaataagc cacagcacag agctttgtga
     6121 aaacttatgg gacatattaa aggcttcaaa tgaatatgaa ttagagcttg aaagagcaat
     6181 tgaaatatat gaagttgttg attgtataga agattttaag aagctgttaa aatctgaaag
     6241 ctactatata atagaagctg ataatgaatt aaatgcttac attgagtttt tagaagaaac
     6301 agggttttta gttggagtac ctgaccacat aattaattat attgactatg aagcactatt
     6361 aagggatttt gaattcaaca atggatctgt taaaagatta aatgaaagcc agtacataat
     6421 atattaaaag aaaaccagct gaaaggctgg ttttttactg ttcaaaaagt ttataaattt
     6481 gtttcaaaaa agtttataaa agtgttgaca tatctgtttc aaattattat aataataaat
     6541 gtagtaagga actacaaaaa cacttaacag aaaagaattt aataaagaag gaaggtatta
     6601 aaagatgaaa aaagaataca aaggattcac aataatttta ggaagggatt gtttaggctc
     6661 tgaaagctgg gttgtttaca aggatgggga acagcttagt gattgcctag actgtgaaac
     6721 tgaagctatt gagtggatag aaaactatat tggataataa aattggaaca gctgaaaggc
     6781 tgttcttttt atttaaaata gcattaaaaa gttttattaa aaagtgttga cattgttgtt
     6841 tcaatttgat ataataataa ttgtcaaggg gaacttgaca gacagaaaac aacttaaaga
     6901 aaaggaaggt attaaaaatg aacatcaaag cactacaaga ttacatcaag tattgccaag
     6961 caaaggaaat tgaatgtact tggcaaggac taaaagcttt ctatcaatta cacaaagaat
     7021 tgtatagaat agcttaaacc caaaggaagg acagctgaaa agctgttctt tttttatttt
     7081 atatattata ataaagtata gaaaacaaat agaaaggaag cttggagcat gtcaggaact
     7141 tttattatgg taattaaaga taaggggcaa atgtttgaag aagtccatta ctgtggtaat
     7201 gatgaaatac aagcccaaag aagatataaa agacttgaag gctgtcaaag acttatggta
     7261 agggcagaag ttaaaagaca aatgattcaa aatgttcctt ttatcatgtc ttatgaagta
     7321 attgaagtaa ttaaataatg aatataaata atatacattt tattaatatt gttggtgctg
     7381 gaacttttaa agttaccagc ctttaatttt ttagggggta gggtatttta agtttggctg
     7441 tttatatgct gaagcacttc aagatcataa ttaattttag tatattttag ttttacaaat
     7501 ttaatcctat ccaatgacca tatttttata ttttattgaa aagaatcttt caaaatatgt
     7561 gagatttcaa aggatttgct gatttttaaa aaaattgacc tttcagaatc cattctattg
     7621 gctttttctt aatgtggtaa tatgtttata ccttttgaac ttaaatgctt tctatgagct
     7681 ttctgtggct tcagaagata attaatattt tttcattgtt ttttacagtt atttttctat
     7741 aaattttcta ttaattttta tgttttttac ttgttaaata atttttttaa acttttaggg
     7801 aatctattta ctgttatagc tgttttataa aagatttaat aagctgttaa tgttcattat
     7861 tttgactttt atttttctgt cattgatgtg ctgatttatt gatttataaa tttattttga
     7921 aaattatttt tcaattgatg taaatttatt gaggtatttt ttcttatttt taccttgttt
     7981 gagctgattt ttttgtgata aatcacttgt ttgaactgat tttttttatg ataaatcact
     8041 tgtttttagg ggtgcatgtt gcatgtggaa atgaaagtaa ttttacagta aatagtgcct
     8101 gtttttagtg tgattaaaca ggctttaaac tgttgaattt actgtatttg atgtatttta
     8161 attttttaat agggggtagg ctgttttgaa ctttgaaaac ctaacaagtg caggactgag
     8221 tgcatcacag aaaaaatgtg ctgagttact tgtaatgaaa gatattaata aaatgtcaat
     8281 gaatcagata gctgaagaag ttggggttaa tagaagcaca attttcagat ggaagcaaaa
     8341 caaagaattt aatgactatt taaatgcttt agctgatgaa ttccaaagaa gctttcttgc
     8401 agatgctttt ggggaattaa gaaacatatt gaagtatgga aaaacccatg agaagttaaa
     8461 agctgttgaa ttaatattaa agaaccaagg taaattaaaa gataatacag aggttaatgc
     8521 cacagttaaa caagaggtta gtgttgatga attcttaaaa caactggggg tataattttt
     8581 aaaggggtgg gggtattttt aaaaccccta ccttttttat tttagggata ttattttttt
     8641 tatgggggct atatttaaaa tagctggggc tatcttttaa agggctgggc ttatttaaat
     8701 atagtccctt tatttcttta tacataagtg tttcaattcc ttggttttga aacattataa
     8761 tatgaacagc tgaaagcctg attattacta catcactttt attatgtgaa acagactttg
     8821 aaacataaac ttattttcta atcatttgaa acatattaaa cagtattgaa acacattgaa
     8881 acagtatcaa gataatataa tatatataag tagaatggca cagcttacac acatacagca
     8941 cccacacaca gctgttatat agcatgataa tatagaccac acagcacagc agatattagc
     9001 atacaaaagg aaaaaaaaat aattgattaa aacattttaa tttatttaat taattcactt
     9061 tgcaaatatt tatttaagct tataaaagac tttttatatt tgaaaattta aaaacaaaat
     9121 cacaaaaata tttatttgtc aagacatggc taccaagggg gggacacccc ctgtatagct
     9181 gggaaacaag cctattaaga atatctacaa taaaatttaa gtccatttac agttctaacc
     9241 agtagagcat gctccacaca ccagccacac acaaccacac atcagccaca catagttcac
     9301 ataatataat tactgaatat aaacaccata tactgtataa taaattcctt gaatctttaa
     9361 taaacagcct taactgtatt taatgtttaa aaaatttata ctaaacatac aactatatat
     9421 ataacttata ttaactttat ataaacagta tattatgtat aaataatttt aacatcaagt
     9481 aaggtcacag aatacaccta attcattaat ttattaaatg ataaatcaag aaaggtggga
     9541 ttctatgaaa gtgttttgtt taacacataa tagaatgttt agaagctatt cttcagctgt
     9601 aaatgataag agcctgaaga atctaacaat cagcaacatt ataaaatgtt gtaggggtga
     9661 aattccttca gctggtaaag atcttcaagg caataaactt caatggtgtt ttttcaggga
     9721 ttttattaat cacaaaaact ttccttgtat agaatcagaa aaaagaagct gggtgttatg
     9781 tctaacagat ggaaggctgt tcagaaataa taaagaagct ggggaattct ataatataga
     9841 ttctgttaga atatcaattc aattatcagg aaaaagacct tcagctggat ttaatgaagc
     9901 tggtgaaaag ctggtattta tgaaatataa tgaccttgtt gaagctatga agggggaatc
     9961 tatttaatga agaaagtatt tgcaatcttg agtgttattc ttgggatatt tatagttgtt
    10021 aaaacagctc tttggtttaa taatgtaaga tatgaagaag aaacagttag ggctgttgtt
    10081 gtaaataaag aatatgttcc ttcaagaaca tatacaacat gtattttcac aggaaagact
    10141 gtacttccta ttgtaaatca tacaagtcct aaatataata taacagttca atggggaagc
    10201 ctaactgaca aaatagattc caaagaatta tacaatgcca caaaagttgg ggattctatc
    10261 aacatgaaac atgttaaaaa atataccaaa gatacaaatg aattccttgg ggattctata
    10321 aaacagccat aaaatgggga ttctacataa atgattattc atttatgtgt tcataatcct
    10381 tgtaagggac agcagttcag ctgttccttt ttttatttta tgtttgttga aaattatctt
    10441 tcaattattt taattttttt caaattagtg ttgacattat tgtttcaatt tgataatata
    10501 ctacatgtaa agaacagata taaacaaact aacagaagaa agagggatta aataatgaaa
    10561 gatataacaa aaatatttaa tagaatggaa gcagatatga agaaggctgg attcagtaca
    10621 gaaggaattc acacagtaaa agaagcactt gtgacacttg ctgaaaacat aacacttgga
    10681 gattctgaag caagaaagtt atttgtaaag tatggtgttt atgaagctgg atatataaag
    10741 aaagaattac caatgttctt cagattaatt tctgataaag tagaagaaaa aaaagttaaa
    10801 gaacaaatca agaaaggtgc ttcaaaagaa agcatagaaa gacagaaaaa gcttgaagaa
    10861 cttggtgaaa atagattaat aggttatgca agggtatcaa cacaagacca aaaccttgca
    10921 agacaattta aagctcttga agaagctggt tgtcaggtaa tatttagtga taaactttca
    10981 ggtaagaatg tttcaagacc tgaatacaaa gaaatgatgg aaacaatcaa ggctggtgat
    11041 actgttatag taacagaatt aacaagactt gcaagaagta caactgattt atttaatatg
    11101 atggctgaat ttgaaaagaa aggtgtatca gttaaaagtt taaaagaatc atggcttgat
    11161 acaacaacag cacatggtaa gttaatgttc actatcatgt caggtatggc acagtttgaa
    11221 agggaactaa tgctggaaag acaaaaagaa ggaattaagg ttgcaaaaga aaatggtgta
    11281 aggtttggtg taaaattaaa agaagaagct gacatggact tagcaattca acttgctaaa
    11341 gaaggaaatt atacaatgac acaaattgca aaaatgtgcc atgtatcaag aacaacactt
    11401 tggagaagat taaaggattt aggaataaga aactaataag aagctgaaag gcttcttttt
    11461 ttttatgccc ttcttttgaa tgttatcttt aaatacttgt aaataatttt acattttata
    11521 ggtggaattg taggaagaaa agaaggggga aggatatatg agtaatattc cagcaaatat
    11581 tcaatctaag ttgttaaggc ttacagatgc aaagaataaa attattgatg caattaagga
    11641 agccaagagc aggggaaaca tgtcagcatg ctctattctt atggaaaagt atagagaagc
    11701 tgaaaaacat tataggattt taagaagtga atatgatgtt ttatactttg cttatgaata
    11761 ctttagtgat gaaagaaatc ctgaaaatga aaacaactta ataccagctg gtggtaagtt
    11821 tgaaaatgca ccagcctttc acattgaatt atgtgaaaaa cttgatgaat taagagataa
    11881 cccaactaag aagatatgct ggtcagtacc aaggggacat gcaaaatcag gttatatgtc
    11941 taatgtattc cctgttcatc aaataatctt cagaaaaaga cactatatat taatagtttc
    12001 tgaaacagga ggaatgtcaa aatcatttgt tgaatgggta ggtgaccaac ttaaatataa
    12061 taaaaaactt agggaagatt ttggggaagt tctttctcct aacaagatgc agaatgaaca
    12121 ggataaccaa gaaggcttct tgacacatac aggaatcaaa gttcaggcat cttctattgg
    12181 ttcacaatta aggggttcaa gaaatggatc atacagacct gaccttgtaa ttcttgatga
    12241 tttagaatca agtaaaaata caaatacaaa ggaattaagg gaaaagaatc ttcattggtt
    12301 caattcagtt attatgccaa ttggagatat aacaagaact agctttgtat atatgggaac
    12361 tttggttcat ggtcaagggt tgcttccaca tgtacttcaa aggtcagact ttgatggaag
    12421 aatctattca gcaattgttt ctgaacctga aagaagggac ttatgggaac acatagataa
    12481 gattcttcat gatgtagaaa atccaaacag ggaagttgaa gctgataact tctattttga
    12541 acataaagaa gaaatggaca aaggtgcttt gacactttgg aatgaaagat ttacttatta
    12601 tgaattaatg aaaataaagg ttaatgttgg ttcaaaagca tttgcttcag aatacttgaa
    12661 caagccttct gatgatgatt catgtatatt caagaaggaa tactttgttt tctatgatga
    12721 acataagatt gatttaaaaa agcttgatat atattctttt tgggatattg caattggtaa
    12781 gagtagcaga agtgactaca atgcaattct aacaatagga agggacaggg taacaggagt
    12841 tatttatgtt cttgatgctt gggctgaaaa gattccaatg cacaaggctt tggatatggc
    12901 tgaaagaaaa attaggcaat ggaatccaaa aatctttggt gttgaaactg ttcaagcaca
    12961 atatgatatg tatagacagc taagggaaag attaatgaag gctggtatat attcaacaag
    13021 acttcttgct tttaatccta aaggtaagaa agaagataga attgaacagc ttgaaccact
    13081 tgttgagggt ggttatttaa ggtttaataa aggtcataga ctgttacttg aacagatgga
    13141 actgttccca agtcatgatc atgatgacct tccagatgct ctggcttcag ctattgaaat
    13201 agctggaaaa caaagaaaga aaacttatta cagaaagcca aaaggttttt aatcttaggc
    13261 tttctttttt attgaaaaat aactttcaaa ctacataaaa aggagaatgg aagtatgata
    13321 aacagtaata aactctttga aataggtggc ttattcccac cacaaaagca ttatgaaaga
    13381 ataaagggat ataaagaaaa taaaaagcta tttaagggac atcattgtga agtatttaaa
    13441 aataatatgc ttcaaggtga tagagcaaag gaacttttat atatatcagt taatttggct
    13501 ggaatcatat gcaaaaaatc agctgatttt ctttttggtg aagagcttag tgtactagct
    13561 ggtaagggtg aacatacacc tgaacaaatt gctttggata gatttatgga agaaaaccat
    13621 atgaatatat tactttatga aaatgcaatt acaaatgctt atgctggtga tacattcatc
    13681 aaagtaagat atggtcaaga atttgctggt gaattaccac ctgaacttga tgaacctagg
    13741 gtgataattg aaaatgttaa tcctgaaact gtattccctg aaactgttgc ttgggataaa
    13801 agcaaaataa aaactttcca tatagctgta ccaatatatg atgaagaagc aaatcaatgg
    13861 tacttaaatg ttgaatccca catgtctgga aagattaagt attgtcaata tacaattaca
    13921 cctattcatt ataattcaga ccaagagcct gaaagatttt caattgatgg tattgttgaa
    13981 aattcagttg ttgaagtatc aacaggggtt tcagtacctt tggttgttca tataccaaac
    14041 ctttcaactt cagatacttg ggaaggtatt gatgatttaa ctgaattaca cccattactt
    14101 gatgaaataa acaatagact ttcacagatt gctgacatat tagacaagca tagcaaccca
    14161 gcaatggctg ttccttcagg tttacttgat gttgatgaag atggaaatgc ccaattcagg
    14221 gttgcacatg ataaagtttt tgaggtcatg ggaaaagatg atgttgttcc acaatatatt
    14281 acatggaatg gtcagcttgt tgaagccttt aaagaacttg ataagcttgt tgacatggtt
    14341 cttgtaattg cagaaatacc agctgttgca cttggaaaag gtgattcagg aacttcagga
    14401 agttcaggac ttgccataaa gtggagaatg aacagcttac ttgctaaggt aaacagaaag
    14461 aaacagtatt actctaaagg attaaaacaa gttttctata tagcccaaaa gcttgaagaa
    14521 gtcattggca tagctgatta taaaatgact gttcctattt tacacttcca agatggatta
    14581 ccaaaggaca acatggaaga agccaatatc atgagtatca gaacaggtgg agcaaaaaca
    14641 atgtcacaaa aaacagctat tatgcaactt aacaacatga ctgaagaaca agctgaagct
    14701 gaaatcaaaa gaattaaaga agaagaagaa gcaaacatgg aaattgctga ttcaggattc
    14761 tttaataatg ctgaaccaac tgaagatgtt gttaatgatg tcttaaaagg ggctaaaaac
    14821 ccctcagaat atgtttcata attaagcaac aaaaacatac cacagactat tttaaatggc
    14881 atacagccat ttctgtggct ttatagaggg gggaagaaga aatgacttat caggaatatg
    14941 atgaaatatt gaaagatatt cttcaaaact ttaataagaa taaacctgac attccttcaa
    15001 gggttttaga aggttcaatt gatgagatta caagaactta tggaagggct tataaaaggc
    15061 tttttagtga actattagaa aagctttata ctgattttgg tgttctagca aatccaagtt
    15121 atcaatcaca gttgggattg ttaaaaatga tagaacttag aatgaaggaa ttaaattcag
    15181 aagtagctgt tcaagtacaa aaggaacttg aaagggctta tgtgtcagct ggtgctttcc
    15241 atgtgttagc aactgaaaac attaagagca ttgaagaact tcatggagct gttccttata
    15301 ctgaattaaa tacatataaa atggaacaaa tagtcaaaga tacaatggaa gatttacttt
    15361 ttgttacaca gcacacttca aaggaattaa aaaaagttgt caggtcaaca ttctcaaaaa
    15421 atatacaata tcacagtttg attaatgaat cacagaagaa tgttaaaagg attatagaaa
    15481 gggacttgtc caagaaattc ttgaaggaat cacttcagaa gaatggattt gttggaatag
    15541 tagattcagg gggaagaaag tggaatctta aaacttatgt tgatatggct gtttcaacta
    15601 aaatgaatca agcatataca gaaggtttaa aagatagagc aatggtcaca ggaaaagacc
    15661 ttgctgttat tcctgaaaag ggtgcaaaag attcatgcag ttattttgaa ggtatgctga
    15721 taagccttac aggagcaact gaaggatata tgacttatga acagctacaa agcacaggat
    15781 taatatttca tccaagatgt gttcactctc ctttccctgt tggaagcatt gatcttgttc
    15841 atgaagatga tattgccttc catgaaaaga agttaaaaga attaaaaaat tttaaaaaat
    15901 aattttaaaa atttacttgt aatatatcac atttattaaa ggtggatatg taaaagcaag
    15961 aagtggaaat caagggctga aatgatgccc ttccttcttc ccttccttgc ttaatatttt
    16021 atattgaagg gctgattctt aattgaacag cctttcactt ttttgaaaaa attaaggggg
    16081 aagcaattat ggaagataag gacataaaaa cacatgaaga aaagatggaa gatgaaaaag
    16141 aagaaacaaa agaacctgaa gatgtaaagg gtactgaaga aaagaaagaa gattctaaga
    16201 aagtgtctga agaagcaaaa gatgatgttg aagctttaaa acaagaactt gctgaatcac
    16261 aagcaaaggt acaggaagtt gaaactttaa caaatgaagt aactacatta aagaatgaaa
    16321 ttgctgacaa agatgcacaa ataaaagaat atgaaacact tgttgaaaat atgattgaag
    16381 ctaagatgaa tcaagttcca gctgacttaa aggacttaat acctgacaac atggatttaa
    16441 aacaaaagtt agcttggtta gagaaggcag aatcaaaagg tatatttaac aaagaaacaa
    16501 agaagaagcc aactattgaa gttggtaagc caatgaatgt tgaaacacca actgttgata
    16561 cttcaaaaat gacagctgga caacttttaa agatggcata ttcaagtgta aaaaagtaat
    16621 aactaaagtg catgataaat tgaaatcaaa gtggcttggg gtaatttaga gttccaagtc
    16681 ttttttttat ttttaaaaat atattgaaag ataattttca aaagtcaaat atggggggaa
    16741 ataagtatgg gtatgacttt aatagaagcc aataagttga cacaagatat gttacaaaaa
    16801 ggtgttgttg aaactatggt aaaaacttca ggggtattac aaagactacc ttttattgaa
    16861 gtagtaggtt caggatatgc ttacaactta gttactgagt tacctgaagt tagttataga
    16921 gcagtaaatg gtcaatataa agaatcaact gttggtgctg aacaaatggt tgaacactta
    16981 gtaatattag gtggagatgc agatgttgat gtgtatttaa ctagaacaca ttcaaacata
    17041 aatgatttaa gagcaatgca aactgaatta aaagcaaagg caacagcaag acaatttgaa
    17101 gttgacttct tcaaaggaac tggtgcttct gatactttaa aaggattaga tgcaagatta
    17161 gctgaaggaa aagtgggaac tgaaataaaa tcagaattaa aatatgaggc tttaaatgaa
    17221 ttattagatg ctgttgttga tggtgctgac tgtttattca tgtctaagaa aatgagaaga
    17281 aagttattaa ccttattaca aaaggatggt cactacatag aaaatggttc agatgctttt
    17341 ggtagaccta tttcaatgta tggtggtgtt gaaatagttc ctgttgagga ttctttaata
    17401 cctgaaggga aagtttatgc tgttaaattt ggtactgacc aatatgtgca tggactttca
    17461 aatggtgggg ttcaagttag ggacttagga gaacttgata caaaaccatg tttcagaaca
    17521 agaatagagt tctactgtgg attagctaca aagcacacaa aatgctttgc tgtgttagac
    17581 actaacactc ccagctaaag ctgaaccttc tgttatgatg gctatgcctt cagaggtaga
    17641 gcctgacaca actgaagaag ttcagccaaa gaaaacaact agaagaagaa caacaaaatc
    17701 ttctgaagaa gatgttgctg aataatcatg ggaaggtgta gaagccttcc ttaatttttt
    17761 gaattcaagg ggtgttgctg gtggatacag taaaacaagt taatgacttc attgctgata
    17821 atatacttca ttcagaagtg tgggattcag caacagaaca gcaaaagaaa aaggctgtaa
    17881 accaagcaaa taggactttg aaaagaatgt tccctaaagt atatacagca gaagttccta
    17941 ttgaacacct tgctgaacaa acactttgga tattaaaaat tgatgatatg ttccaaagag
    18001 ctgaactggg agcaacatat attgctgttg atggggtggc aatgaatatt gctaataaag
    18061 acagaagtat ttgtccattc ttaatggaag ttctaaatct ttcagaaggt tggaattcaa
    18121 gaagaaaagt tggaagatat tccacatata tgaaagattc cttcagaaag ggttggtagt
    18181 atgggactta aatatatttt agataatgaa cagatatttc acattaaaaa tgttggagtt
    18241 gatgcttggg gaatcaattc ttcttccttg gaaaaaacag cttatagttg ttgtataaga
    18301 ggggctgaag attcaacacc tattgaagca caaggaggta aacaagtgat tccttcttat
    18361 acagtttctt ttaatagtaa agttccattt actgttgggg aaacattaga agtagaagga
    18421 aataggcttg ttattttaag aaagaaagaa gttaaggact tatcaagaaa ggttatgttc
    18481 acaaaggtta ctttataggg ggtgtggtac ttgataaaat cagttgttga atttccaaaa
    18541 ggtatatttg aaattgatag taatatgatc aatgctgtaa acaagggaat gaatgaaata
    18601 actgatgatt tgttaagggt agcacaacaa aggtcacctg ttgatgaagg cacacttgaa
    18661 cagtcaggaa cttccaaagt tatttcaagt aaaagaatga ttaaaggttt ggtttcattt
    18721 tcagcaagaa acaggggcta taattatgct ttaaagatgg acaaaggtaa atataatctt
    18781 ggtaaaaaat cacttagcaa atcttcaaga ggtgtcagaa gcaaattttc aaaggtttca
    18841 atgaaagtag gaagtggtta tttaagtgat tcagctgaaa agtgcaaaga tggttatacc
    18901 aaacacatca atgaagagct tggaaaagca attatgaagt caggcttcaa attaaaatag
    18961 gggtgattca atgagtattg tatcattggc tgaattcatg tcagccttag ttggtcacca
    19021 agtttatgca attaacttcc ctaattataa gaagggggat tttctaaagc ttgaaattac
    19081 ttcaggagtt atggaacttg gtggggttga tgattttaat gttcaattca tggcaagagc
    19141 atcccaccca gcaaaagctg aacaaatgtg tcttgatgta ttggacaagc tagataaatt
    19201 aacaaataaa gaatttaatg atggcaagtg tcagttgata cttgcacaag ctgaagcacc
    19261 acagcctttt tatgtaggag aaactgaaga tggtgctttt ttaatgtcag taaattttag
    19321 actgctgaca acaactttaa cataataggg ggaatttaga tggctggtaa aaaggtagct
    19381 ggtgttgatg tccttttaaa agtaaaaggt tctgatggtc agtttttggc tgttggtgga
    19441 caaaaagggg cttcccttaa aagacaagct gaaacaatag atgtttctga caaaacttca
    19501 ggtggttggt cagaaagtat aatgggactt aaatcttggt cagtagagtg tgatggtttt
    19561 gtcactttag gtgatgctgg attcaagtta gtccatgaag cttttgataa cagaatacct
    19621 ttagaggttg aaataaatgt tggtgatgaa gggggatatt cttacacagg aagagttgta
    19681 attactgact tccctgaaga atatgcttca gatgatgctg tcacttatag cttatcactt
    19741 caaggggctt cacctttagt aagaaaagat aatggagcta cttcacatta atctttgaaa
    19801 gatatttttc aatagtcagc tgtcagccct gacttaaagg ctgactgttt taattaaaac
    19861 aataaaaata ttaagtacat aaaaggaagg ttgattgaat tatggcaaaa gaaagaaaaa
    19921 tggtaactat aaatttagat aaagaaagac acttatattt cacattaaat tcattggagt
    19981 taatagaaga tataacagga acttcaattg ataagatagg ggaaaccatg aatatgaaaa
    20041 ctttaaaaat attattatat gctgggttag ttcatgaaga taaagatctt actattgatt
    20101 cagtagggga tatgataggc attgaaaaaa tagaagaagt ttcaaaagca ataggggaag
    20161 ctttcaaagt tttgaagtaa tcctttcatt aggaagtaca aaagaaatta agttttgggg
    20221 gtatggcaag ctaaagctta ccccaaaaga tttgtatgaa actgatttaa ctgttttagg
    20281 ggactgtata atggcacata ttagggctga acaagaagcc attgacagtc aaatgcaaat
    20341 gttagcatgg cagacagcct tattaatgaa ttcaacaggc aacttcaaga agaagattaa
    20401 gccaacagat ttatatacac ctttggctga aattgatgtg aagcagaagg ttgaatataa
    20461 gcctgaagat aaaaaagccc tacaagaaga attgctttca gcttttgctg gttcagacat
    20521 agttactcaa taaagttggc tgttgggaag aagattcctg acagccttct tttttttatc
    20581 tatttttaaa aagggggaat cactaaatgg cttcaaatga agtattgatt aaattggttg
    20641 ctgatgtttc cagccttcaa gcaggcatga agaaggcaca acaggaatta agcaacttaa
    20701 aaaatacagc tgaatccagc acaagtggaa ttggtaaagc tttaggaact gttgggaaaa
    20761 taatagctgg tgcttttgct gtaaacactt taaaaaattt tggtgctgat gttatcagaa
    20821 caacagcaac attcaatgat tcaatgtcaa aagtaaaagc tcttacagga gctactggca
    20881 aacaatttga ccaaatgaca gcccttgcca aaaagatggg ttctgaaaca gcccattcaa
    20941 gttcagctgt tgctgaagct atgtcatata tgggacttgc tggttggaat gctaatcaga
    21001 taatgtcagc acttccttct gttctttcat tagcttcagc tggtcaggtt gacttggctt
    21061 ttgcatcaga tatggttaca gataccatgt caatgttttc attaaaagct gaagaagcaa
    21121 caagggcttc agatgttttt gcaaaagttc aagctaagtc aaatacatca attaaccaac
    21181 ttggagaatc aatgaaatat gttggagcaa cagcaaatgc ttttggactt gatattgaaa
    21241 caacatcagc tttattaggt gttatggcaa acaatggtat aaaagggagt atgagtggta
    21301 cttcattaaa gacaatatta tcaagacttt cagcacctac aaaagaggtt tcagaaggat
    21361 ttgatatgtt aaatatttcc cttactgatt catcaggaca catgaagcca ctaactgaat
    21421 tacttcctga aataaagaaa aagatggctg gtctttctaa tgaacagcaa attgctgttg
    21481 ctaaaaatat agctggtact gaagcaatgt caggtttcct tgcaattgta aatgcttcca
    21541 cagatgacct tccaaaactt attgaagaac taagaaattc aagtggtttt gctgagaaaa
    21601 cagccaagac aatggaggac aacttaggtg gagctatcag ggggttaaag tcatctttag
    21661 aaggagtaaa attagcactt ggtgatgcta tttccccaat tttaattcct attattcaag
    21721 gaatggctaa tgctcttaga cttattcccc caactgtgac aaatataata acatttgtaa
    21781 caccagcttt taagggctta ggtgacataa ttaagtcatg caccaatgtt gtcaaggact
    21841 gtgttaattg gtttaaagag cataagcttg ttgcacaagc acttacagga attgttattg
    21901 gacttgttgg ggcatatgca acatttaaaa cagcattagc tgttactaat ggacttaaaa
    21961 caatgactga cttattagat acagcatcta ttaagatgtt attattccaa gacagaattg
    22021 ctggtgcttc cttatcaatg acccttgctt cagcaaagac agttctttgg aatactgttt
    22081 cagccattgg tactactgtt acaacagcat ttggaacagc attgtcactt ctaacttcac
    22141 caataacttt ggttataggg gcaattgtag gacttgttgc tatcagttac ctgttaataa
    22201 aaaattggga cacagtaaaa gccttccttt caacttgctg ggaagcaata aagacctttg
    22261 caataaatac atggaatagt atgtgttcag ggatttcaag cataatggca agtataaagg
    22321 gagctgtatc aaatgtatgg aatagcattt gtacttggat ttcaggagtt atgacaaaaa
    22381 taagtgctgt tgtgtctagt gtatggaata gtattaaggc tagtatttca acagttttaa
    22441 atgtaatcag ctctataatt tcaactgtat ggcatggaat tcaaattggt attgcctttg
    22501 tacttaactt aatattagct gttattgttt ctatatggaa cttggtggtt acagcaattt
    22561 caattccatt aaatatcata tacaacattg taatgatgat atggaatcag attaaagata
    22621 cagttatggg ggttttaaat gcaatccttg atgttgtatc agctgtttgg gtagcaatta
    22681 aagataaaat tatgggggtc ttaaatgcaa tccttggaac tgtaactact gtgtggaact
    22741 caataaaaga agctgtatca actgttgtta atgtaatact taatattgta actactgtat
    22801 ggaacagtat tcataataca atatcagctg tattggcagt aatagttaat gtggtaacaa
    22861 cttactttaa tgtatataaa tcaattatta tgagtgtttt aaatataatt aaaaacatca
    22921 tcacaactgt atggaatagc atcaaggctg ttatcaacac agttttaaat ataattgagg
    22981 aaattatcac aactatattc aatgcagtaa aaaattacat tacagctgta tggaataata
    23041 taaaagccat cattacatca gttttaagtg caattaaaag tattgtttca tctgtatgga
    23101 atagcataaa ggctgttatc agcacagttt taaatgccat caagagtgtt gtttcatctg
    23161 tatggaatag tatcaaggct gttatttctt cagttggtag tggaattaag tcaactgtat
    23221 caggggtatt taattctatt aaatcaattg tatcagaaaa aatgaataca ttgaagggca
    23281 tagtttcagg agcatggaat aggattaaaa gcatctttag tgctgtgctt aaaccaaaca
    23341 tcaaattacc acaccttagt atatcaggga agttcagtct ttcaccacct gaagttcctc
    23401 accttggagt aagttggtat gcaacaggtg gtattgcaac agcaccttct attgtaggta
    23461 taggagaagc tggagatgaa gcaattcttc cattgtcaaa taaacacaga atgaagccat
    23521 ttgcacatgc tgttgcttca atgatgcctg ataatgtgaa aaattcttct gatggtggca
    23581 atgtgaacat aaatgtgtca cagcttgtgg ttagagaaga agcagatatt caaagaatag
    23641 ctgaacaact ttataagcta caagaaagaa acaaaagagc aagggggaaa tattaatgat
    23701 gaaattcatg gtcacattta atggtgttga catgcctgac tgtttaaagg ttaaggctgt
    23761 caatacttca atattacctg aagttaaaac ttcatataaa gatgttgctg gtgggtttgg
    23821 tgtaattgac acaggaacaa ccattggtgg aaagaccatt tcccttgatg ttgttatagt
    23881 tccaccagct ggaaagtcac ttcttgaaat gcaaagagaa gtggcttttt ggttaatggg
    23941 gaatgatttt aaattatcac ctttagtcat aagtgaagaa tgtgatcttg agtatatggc
    24001 aaaggtcaat ggggctgtta atataaaaga ccttttcttt gttggtgaag gaactattga
    24061 cttctatgtt ccaaaaggaa ttgctgttgg aaggtttaca agatatggag caaatgaatc
    24121 aggaaataaa atcattgttg actatttagg gacagcccct tcatacccaa tttttgaatt
    24181 tacaccaaca aaggattata aaaatactac tctaagaatt gcacatatag aaacagggga
    24241 cacaacactt attacaggga attttaatgc tggtgaaaag atcataattg attccacaaa
    24301 aaggcttgta aaaaaggggg gtgtcctatc attggatatg atagacctta aaagtaaatg
    24361 gttgaagctt aaaggaagaa ggataaatac atttacctat aatttagaag gagaaatggt
    24421 gttgtcctat caagaatctt ggttatagaa aggggaaagg aagttggact tatatatttt
    24481 aaatagacaa gaaaaaattg ttggtgttct ttcaaatgaa ggtcacaaca attttctttc
    24541 ttcagctgta atgaaagaaa gcttgaggga aatggacacc ctaacccttg aagtttcttc
    24601 tttaggtata gaagtttcag aagtcaagga agaaaattat gttttatttc aagacataac
    24661 agggacatgg aaaaacttta taatcaggga agtaaatgaa atacatggaa ctgatgcaat
    24721 gaaacaagtc tatgctgaag attcaagcca agaattaatt gattatcttt tttttgaaga
    24781 attaaagggg caaaagctat caccttcttc cctactggga ataattcttc agggtacaag
    24841 atgggaagta ggagaagttg acaaaatttc tcctgttacc tttcctgaag aaacaaggaa
    24901 gaaaactgtc cttgaatgta ttcacctaat tgttgaaaca tttaacctac agttaagatt
    24961 cagaattgaa gttgctggaa acaagataat tggaagatat gtggatatta aaaaacatat
    25021 tggagctaat tatgggaaaa gatttgaata ttccaaagat attgaaaaga tagaaagaaa
    25081 ggtaatgtca acaaggctta aaacagcaat tattcctttt ggtgctgttc ctgaagaaga
    25141 agaaaaagaa gaatctaaat tgaaagataa cattcaaaat attgaagaaa atcaggaaga
    25201 aaaaccaaat aagccaccaa ttgatattac aggagtacaa tggacaaagc ccaaaaatcc
    25261 attaaataaa ccttttggtc agaactatct tgaaatacct gaagccacag cacaatgggg
    25321 ttatatggac aaagaaggta agatgaagcc aagatttctt ctatatgaaa atactgaatg
    25381 taaaacacct gaagaactta tccaaaaagc ttatgaagta ttaagggata tatcaaagcc
    25441 tgttacaaac tataaacttg aagttataga tttgtttgca ttaacaaaag atacagagct
    25501 ttcatttgaa actgtaaggc tgggggatat agtttcagta atagatcatg aattcacacc
    25561 agctattcag ataaagactt caattattga aagggaagtt gatttgcttc ttcctgaaaa
    25621 tacaaaaatt gaacttggtt ctttcattag gaatttggtg gattctgaaa gctcacaaca
    25681 aatacaagac atgattaatg cttcactttc aacagggctt gaagatatag aagcaaaggt
    25741 tgttgatgtg tcaaacactt ttgaggactt caaaaagaac tacacaagta atgaattaaa
    25801 tctaaattgg attaagaatt cagacttcag taatggctta aaatattatg atagctggaa
    25861 tgattcagat ttcagacttg aagctgttaa tgaaatacct tactttgaca actgtggaag
    25921 gttgtcaaaa ggcaacttca cacaagaaat tgaaggagct gaaaagctaa aagggaataa
    25981 tgttactttt tcagcatttg tttggggtgc tggtcattta tcccttgaaa tcacctacat
    26041 aaataaagaa ggtcacttaa tacaagatgt attaaattct aaacagatac ctgaccataa
    26101 gggctggaat agatatagta tgacagtagc cattacacaa caacatgtca gaaaaatact
    26161 tgtttcattt gttcctgaaa gtgattcata tgttacaggc ttacagctta atcttggggc
    26221 tgttccaagt aagtattcaa agaatccttc agacaaatat ggaaaaggtg tttatgacca
    26281 aataagggct gtatcagatg cagaatttaa gaatggactt ggttatgtat atcttgaaga
    26341 agaagatggg ctttgggttt atgataagcc ttctaataat cagccaacta aaatgacagc
    26401 acttaaaggt ggtatgcttg gtattggtaa ttggaatcca caaacaaggc aatgggatat
    26461 aagaaccttc attgatggga acatggtcaa tgcttcttgt atcaatacag gaaagcttaa
    26521 tgctgaccta atcaaaacag gaagaatttc ttcccttgat ggttctgttc aaatcaacat
    26581 ggagaatgga aggttcagtt ttggtgttgg aggttcaggc aatacagccc aacttacaaa
    26641 tgatgaattt aagattcatt attctgatgg ttcatacact caaatgaata aagatggctt
    26701 caggtggtgg aaagctggaa atagtagagc atatcattgt cttgcaacta ctattgaatt
    26761 tacagcaagt ggtgacccaa acaatgatat tactgttaca ttaccaactg aattcagagg
    26821 aaaagacttc acagcacagg ctgttctttc tgatacttac acagattcat gggactgggg
    26881 acaaccttgg gttgttcaaa gaatggtagt tcttgtttca ggaataaaca aagaaaatgg
    26941 aactgttact ataaaaggtt atagaactga taagaattac caaactggtg cttacagaag
    27001 gtgtgctatt agaggtgttt taattgtaat tgcataattg aaaaataact ttcaaagaaa
    27061 ggttgactaa tatggacata aagaactttg aaaaaacaat gactgtgttc ttcagcaaat
    27121 ccacaggaaa aataaaagct ttgtgccaag gggtacaggg ctttaatttt tttggtgatg
    27181 aagctgttga tatggaattg atacttgata gattagtaac tgactacaat ccaaaagtaa
    27241 tgtcaaatat ggacttctat gtggttaaag aaggaaagat tgaagttaat gcttcagttt
    27301 tagagcaatt taaactataa agggggtgag tataggtggc tattaaaagc tttagtttta
    27361 aggttgactt tataacaaag aattcattat gtatggcaca tgtgaaacaa aatgacaatg
    27421 ccatatttaa tatatatctt acacagcaag gtcaggcttt agaccttaca gggcaacaaa
    27481 taaggctatt tgtaagaaag cctgatggaa agcttgttgt tcaggaaaca gggatcacag
    27541 ttgttgatgc caaaggggga gcaataagaa tcaatgttca gaactccatt tttcaagctg
    27601 ttggggttgc tgtttgtgaa atagacctat gggggaatga caataaaaat gcttcttcag
    27661 gtagtttctt aataaatatt gatgaacagc taggaagtaa tgatgttgta aaatcatatg
    27721 ttgatgttaa tttatttaga caattaacag aatatattga tgaagcaaat gctgaaataa
    27781 ttaaatataa gacattgttt gaagcttttg tgaaggctgg ggtatcactt caaggactta
    27841 atgacataaa ggcttacatt gacaacaact tggctgaatt aaaatttgaa actgatgaag
    27901 ctgtggactt aataccaaaa gtcaacaaag caagaactga tgcagaagcc aaaagggtgg
    27961 aagtagtaaa tgccactaat tcagcagaag ccaaaagggt tcaggtggta gatgctacaa
    28021 gtaaagctga agcaaaaaga attgaagttg ttaatgttac agcaacagct gataattcaa
    28081 agaatactct tattaaagca acaacaaatg ctgataataa gaagacagaa cttgaagggg
    28141 caacagcaag tgctgaagct aaaagaaaag aagttgtatc tgttactaat acagcaaata
    28201 cttctaaaag tcagctggaa atagcaacag caaatgctga taagaagaag gatgaagttg
    28261 tatcagtaac agctaatgca gaaaagaaaa gagctgaagt tgttggaact actaatacag
    28321 caaatgaaat taaaaaatcc cttgatggtt caattgcttc aggtaattca gttaaggctg
    28381 gtcttgatgg ttctatcaat acaggaaaca atttaaaagc tggacttgat gcttctatta
    28441 agtcaggtaa tgatataaaa acagctcttg attcagcaac ttcttcagcc aatacaaaga
    28501 gggttgaagt tgaaacagca acaaacaatg ctgaacaaaa aagaattcag gttgttgctg
    28561 ttactgattc agctgaagct aaaagaaaag aacttgatag acttataaca caaattaatg
    28621 ctgaaggtat tatggtaaaa ggaaatattc cagcatcaag ggattgtaat gattttaaag
    28681 attttaacag gtggattgct tgggacacag gagcaggtga ttttaaaaat acccctgaag
    28741 gaacacttac caagggaaca gctagggtgt ttgtaattac aaatagggga tattccccac
    28801 aaagaataca gcaagaattt ataaatgtgt acccacaaaa tagggttaca aggtttgtaa
    28861 gaaactataa tgaagctggc tggggtgctt ggtataaagt ctatgatgaa gctaataagc
    28921 caacaccttc tgaaataggt gctgttaaca aggctgggga cactataaca ggctatttaa
    28981 aactaaatgg ctttgaaaat agtgctagtt atgtaaggtt ggctggtggt agtgaaaatt
    29041 ggcttggtgc tggaactggt gcaacagatg tatacatttg taatgataaa tcccaaaaat
    29101 atttgcaatt aaaaaatgat ggaaggcttt gttatgcaga caatgaagtt tatcatcaag
    29161 gcagaaaacc aacaccttct gaaataggag cattggggaa aacagaaaag gctgttgatt
    29221 ctgataagct tcatggtatg accccttatg atggtaaaaa tggttcaggg attccagttg
    29281 ttgctaaaga tggggttatg gaagttggaa aatttcttga tttccatgct gggggaactg
    29341 gtgactatga tgttaggctt gaagcaaaag ctggtgaact taatgtgtgg ggtaattttt
    29401 catcaaatga cctttgggca agaaaatact tacatgttgc tgattggtac aatccttcaa
    29461 gtggtaatgc ccagttttgg tataagaatg aaaacagaac tttataccta gagaatgccc
    29521 aagaattaat gataaataat tcaagggttc accatgatcc acaggcttct ccattatggt
    29581 caggtggctg gtatgtgact gatacccaat atgtttaccc aacaaagaag ctatcacaat
    29641 gtaataatgg ctgggtgtta gtatggtcag attttgaccc tgatacaaac aaacagaatg
    29701 atacaagctg ggtatgtact tacattcata agaactgttg gcattcaggg gaaactgatg
    29761 tgttgattcc aacaaatgct tttgatgccc caatagtgaa ggcattacaa ttccaagacc
    29821 attgcttcaa tgggtatggc tcaaatagta caggagaaaa agcagatgta attcttagat
    29881 ttgtctatga atggtaattt gaaaaatatc tttcaaaagg agaatgatta tgttagaaga
    29941 aaaaattgtt cagggtgtgg ctgatgcttc ccctgtattc actaaatatt atgtacaaat
    30001 aaatgctgat aatgtggtta tagggtattc aagtacacaa acacctgaag ccacaaatga
    30061 aattgaagtt gaggctgata agctgacaga acagtttatg aaaatgcctt ttttctacag
    30121 atatgatgaa actgtcaatg atttcatctt tgatgattca ttaagaagtg ctgaagctga
    30181 aagaagacat aacaggctta ctaaagaaca acagttaggt cagcatatat cagatttaga
    30241 aatacaatta atgatggtgc aaatgatgat gcaaaagcaa ccacaagcac cagcaacagt
    30301 ttaataatag gggggattta tttatggcag acaattatgt accaagtgga cttgtaaaag
    30361 attggagatt cagatttgta aacaaatggg caactgattt ccacattaac atgctggtat
    30421 ctttaggtgt ggttacagat agagatgcag aatacataag aacaggagtt taattcatag
    30481 acagctttag ggctgtcttt tctattgaaa ggaagggggt gctgtctatg gattatgaca
    30541 aacttctttt ggggggaatt ggtatcttaa caactcttat tggtggttgg gatttaactc
    30601 ttcaggtttt attaatactt atggcaactg atgttgttac tggtgttatt aaaggacttt
    30661 acaaatggga ctttacttca agagcattca gacaaggatt aatgtccaaa gctggattct
    30721 taattgtgat tatcctttgt taccaagttg acactttatt gggtaatgct gaacctatta
    30781 tcagaacaac ttgtgcaatc ttttatataa caattgaagg aagttcaata attgaaaacc
    30841 ttggagaaat gggtgtccca attccaaaag cattaacaaa aagacttgca aaacttaaag
    30901 acatgtcaga tgaagaaaat gaagaatctg accaaaaagg aaaaattgaa agataacttt
    30961 caatcacctt gttaattcct agagcatttg ccctaagagt tgacagggtg atttttttca
    31021 ttctgtaact aatcaataaa ggaggaattc atttgagaat tggaattgat aaaggtcatg
    31081 caataaaagg tgttaggggt gcttcagcaa tacttgatga agtaaatgaa aacagaaaaa
    31141 taggtaatag acttattgaa atgttaaaag aaaagggtca tacagttgtt gactgttctt
    31201 gtgatgttgc ttatgatgtg aatgaacagc ttgctggaat agtttcaaga gccaacagac
    31261 aataccttga tttatttgtt tcaatacacc ttaattcagg tggtggacat ggtactgaaa
    31321 cttatacatt accaaatcca aatgaatcta caaaagctaa agcaaaagca attaatgatg
    31381 ctgtagcttc aagctgtggt ttctctaata gaggattaaa aacagcaaga ttctatgtat
    31441 taagaaagac tattgcccca gcaatacttg ttgaagtatg cttcacagat tcacaagaag
    31501 atgcaaataa gattaatgtt gaagctgttg caaaagcact tttcaagggt ataacaggaa
    31561 cagaatatga tggttcaagt gattcaaacc cttcagcacc agctggttat ggtattgtta
    31621 cagcttcagt tcttaatgta agaaaaggtg ctggaacaca atatcctgta attggtcaac
    31681 taaaaaaagg tgacagggtt aaacttgatg taaaagttgg tcaatggtgg tcaacatact
    31741 atggtgaaca tggtggattt gttcatggaa attatatttt agtaaattaa ttgacaaact
    31801 ttaatatact atgatatact taaaacaaat tttaatacag attcctttct gtgttaagtt
    31861 tgttttctgt taagaacagg gacagccaag agctgttcct gttttttatt tttgtctaat
    31921 taccaattat attcatcaaa gtattcaggt gattgatagc accttcttaa agcatcttgt
    31981 tcaaggtcat attcaacaat tgaagtcatt aatgttttat ttatataccc aatttgtctt
    32041 gaacctattt cagaaacacc atctttttca agtacctttg tttgtaattc taatagcatt
    32101 tttaaatatg gactatcact ccacatgaac tgttcagcaa atgatccata ttcaaatata
    32161 aagctatcaa gcacatatgg acttaatgct gtattttcaa gtaagaattt tctaaataaa
    32221 gctttctttt ctttttcttt aacttctggg ctttcttctt cagttacttc aattactttt
    32281 ggtttaacag cttcagcatc attgtcaaag tattttcttg tttcatggtc aacaacatga
    32341 aatactaatt catggacact tcttcctttt ttgatttcat caactttatt ttcaaaaatt
    32401 atttccatat ttcctgaagt attgatttct tttattgctg gaaggataac tttcttttta
    32461 aaatcagcat aagctgaaaa tttgtttaaa gcctttaaat agcttcttaa tgtcttaaca
    32521 gtaaacttaa tatctacctt tctattttgt cttgtccata gccttataag tgtatatagt
    32581 ctttgagtat atgcaccttt gaagttgaaa agtaaatcaa gatttattgg agcataccct
    32641 tctttttgca tcttaacaaa atcagttata tgtttatgta ttagttcatg aatttcaatt
    32701 atgaaaagtt gttcttcatg gtcatagtga tgtgaagtta taagaccagc attgaaagtc
    32761 attacctttc ctgtgccttc ttctatgtaa tcaaatgaca atatgttagt cttgaacata
    32821 tcaagctttg cttctattga ttcaactgta taatcagcct tttttttcat gaaagttctt
    32881 atttcttcaa cagtaaggac tgttttgtat aaagaatctt ttgtatgttg cttttgtgca
    32941 ttgtataaaa ttttatagaa taatctattt tcaatagctg tgaaatcata ttcactttta
    33001 attaaattat ttggttgcat taaaacacct tgctttatat ctgacattat tgtttttttc
    33061 cacctttctg ttgtttgttt tctgcaattt cattatagtg ttttgaaaca actttgtcaa
    33121 catttttttc ctaaaaattt ttttctttta aatcatttcc taccttgcta ctttttattc
    33181 atttttgaaa gttaattttt caatttgaaa attatctttc aatttgaaag ttatctttca
    33241 atttctgtgg atatgtggat aacatttatc aacagccttt agccctttat ttacctttgt
    33301 ttgaccacat ttataaaaaa taatttccac aagtttttcc acagattttt gtgagaaagt
    33361 tcccaaaggt catatttcag ttcccaaagg tcatatctca gttcccaaag gtcatatttc
    33421 agttcccaaa ggtcatattt aagttcccaa aggtcatatt tcaacttctg aaagcattga
    33481 tttgactgac tttctgatac ccctaaaaac tattaaaaac tattaaaaac tattattaaa
    33541 aactatataa atatataaag gtgaggtata ttatatatat atattaaagt ttaataaaat
    33601 accttacctt acctattttg tcaataggaa aaataaaaat cttttttatt agcttttaaa
    33661 acaaaaactt ataagcatta ttgttttact taaaaagttt ttagaattaa acagcatata
    33721 acagcataat ttttaaaatt taaaaatata tttgtatttt gacatagaac ttaaaggtgg
    33781 aaatagtaat ataacagcca taaaggggcc tattttcaat tttagggggt agggtagtat
    33841 gttttatcct ttaacataat ttaaagccct tagaattgat tctgaggctt tttatcatat
    33901 atcaaaattt atgataaaaa tatttaaaca aaaaatactg tttatttaat taaatataag
    33961 ttttatatat agctgaatgt ttagttaaaa atatttaatc attaggctaa tgtcacatag
    34021 gctgttccac tattaattta aatgctgtta agacaaagct taaaggaggg tgcatatggt
    34081 aggcagtaaa aatataaaag tggaattaat aaagcaatat gatgttcaaa caggtacata
    34141 ccatgaagaa agaaatatca aagtgaatat gcaatcaata caagctggat tgataaaaac
    34201 tttaggagca acaccatttg ctgttcttat ggcaatagtt tcattctgtg acaatgaggg
    34261 ggaagctttc ccaagtcaaa gaaagttggc taatataaca ggactttcat tgccaacagt
    34321 tcataaggct attaaggtat tgcttgaaac agaaattgat ggtgttccaa tactatcaag
    34381 ggaacttgaa actttgggaa gtagaaccaa gttttctgtt tacacaataa caccaaagaa
    34441 agatgcttca ggtaaaccaa agaaaaagac ttcaagggat tatgttctaa agttcaaaga
    34501 tctatatgaa aagacctttg gattcaactg tgttgtcaac tatggaaggg actgttcact
    34561 tgttaagaat aagctaatgg caaacttcac agaagaagaa gttgatatga taattgaaaa
    34621 gggggttctt gagtataaag acagatgggc aaattcaaat tatccatatc caagtttaac
    34681 aatggtgtgt acttggatag ctgatatact tataaaagag cataaacaag cccaagaaaa
    34741 tgctgaagaa cagcaaagaa tcatggaaga agcacaccaa cttgctgaag ttgatgatga
    34801 tagaataaat aagttctttg attcaatata gaaaattcat agaaattcat agaaaagtat
    34861 agaaatttat agaaataaaa tagataagga caagctattt tgcttgttct ttatttttta
    34921 tgtgtagaaa attaatagaa attcatagaa aagtatagaa aagtatagaa aaggggttgc
    34981 actattccca ataaaatggt aacattatac atggaaaata catcttaaca tgaaaggaag
    35041 attgaaataa tgaaaatcta tacaggaatt gatgtgggaa atgggtattg caagtacaaa
    35101 ggtggaaggt ttgcttcaaa agttaagcat ggaagccttt caaaagtagg agcaaaaaga
    35161 aagaacatac accaaatagt gtataagggt aaagaataca ttgttggtga tggtggaact
    35221 ttcacaggaa acaaaagata ctatactgaa gaatatgaaa ttgcactatt aacaggaatt
    35281 gcacttgctt caggagggaa agttgcaaca atacaagcaa atgtggttgt tgggcttcct
    35341 gttgatcatt ataagatgaa ttctgaagaa gttcaaaagc atttaaacaa cttaggtgta
    35401 aaagaaatta ctgttgatgg taaggagtat ataattgaat ttgaatcagt aacagtattt
    35461 attgaagggg catatccaat tggctatgca gatgattcac atatgataac aattgacata
    35521 ggtgctggaa caataaatgt tatagaatgg gaaaaccaag aaataataaa taagtttact
    35581 tttgaccaag ctttctataa gatgcacaaa gatttggctg aattcctaaa tgaaaattat
    35641 ggtataggaa taacaccaaa tgatgctgaa aagtatgttg gtaaaagtat tatagataca
    35701 gaagaagggg aagttgaagt tcctgaaata gatgaaatag ttggaagctt tatatcaaac
    35761 atagcaacta ccattgaaaa cagcttccac actaaaacag ttaagaaaat aaaaattttt
    35821 ggtggtggag caaaagacac ctttcaacat tggaagaaga aatttgcaaa ggctgaactt
    35881 gtaaaagaaa gtcaacatgt taatcaagct gtttatcagg ctgtggctga atcaatggct
    35941 gaagaagagt aatgaaaaaa agaaaaccaa ggaaagatgg aagatattca attaacattt
    36001 cctttacaga acaagaaatt gatttaattg agcatgtaga tgaaagggga aacttttcag
    36061 cctatgttaa gtatttaatc aggaaggaca tggaaggttc aggagcaaat gctcttgacc
    36121 ttatccaagg acttttgaaa gataacattc aaaacaagaa acaggaacag ccacctgaag
    36181 aaccacaaaa gccaaaagct aataagagca agataaataa gatattgaat aaaagtaatt
    36241 tataggtgct gaacagaata agttcagcat cttttttttt atttttttaa aagtgtcaca
    36301 actacattca cataattaat ttagtttatg taagacaaat aaaaatgtga aaggtagggt
    36361 gttacatatg aagaacacaa aaatgatgaa aatattaaat gaagaaatgg caaagtttga
    36421 tgatgtatca aaattggaag aagtaaaaga aactgttact ttaacaatga atgatgcagt
    36481 tgaggaaata aagaattcag cacaggaatt tgcttcaaca atgtctttaa ctgaaatggg
    36541 attaatagaa gttactgatg aagtaagaat gtcagtaata gcaacaatga ttaaaggtgc
    36601 aattgctgga gctgttgttg aggaaataac taaccaatta aaagaaatag attctgttat
    36661 agaattcaac aaagaatttt tagaagaatc aaaagtgact ggctttgctg aaaaggaaac
    36721 aaaagatgaa gatgaagcta aagttgaaaa tgaagatgta tttgagaagt taattagtga
    36781 attaataatt ggggcattaa tagaaggtat agaaaatatt aaaaaggaag ttgatgggga
    36841 agcttaattg cttcctttca ttataggttt tgaaagatgt ctttcaattt aacagttaat
    36901 actttggaat tttggttgaa acaacctgaa tttgcccctc caaagataaa catgacaaga
    36961 gcagaaacaa atgacagtaa ttataaactt atggaagcta ataacagaat attaggaaga
    37021 agcttccacc aaacattcag caaatggttt aagtcttata aaagaattcc aactccacag
    37081 gaatttattg caatgcaaat gaatgatgtt aaaaagaatt ataatacacc aacttggaga
    37141 aaaactcaca aggtcacttt ccaatggaca ccaacagttg aaaagggaat taagcaaagg
    37201 ctgttaagaa gctatatatc atttataaat gaaatacaca cagaacttat gatacaggac
    37261 ttatacacag ggattaagat tcaaagaagt gataaacttg actattcagg aattgacatt
    37321 cttgctattg attcaaaaag taaagttaca catgaaattc atatcactaa gaattcaccc
    37381 tatgcaatag actttttatt caagaaggaa ggaaaggaat tatgcttcaa aaggcttcag
    37441 aataagctgt gggcttatcc ttgttggaat aagataaatc attcaatata cagtaaaaga
    37501 agttttagag ggcatgtatt tctgttatat gataaccatg aaacatattc aacaaaaata
    37561 ataaatggtt atccactatt taaaaaagaa tatatcatga ataaattgga cacaaactgt
    37621 tatttaaagg taggagtata aaacttctat ctttttttta tgtcacaaag aaaaaagcct
    37681 ttttaattta tgaaatgaaa tcaaaaagaa agggctgata aaaatgctta gtgaaaaatg
    37741 tatgttcagt aatacatgta aaaataaaga tacagactta tgcaatcctt tatgttaccc
    37801 atttattgtt ttacatggtc agaaaggtga aacaggcttt tggaaggctt caggagttcc
    37861 aaacaagtat aaaaaagtaa tgtttcacaa tttaccaata aaagctgaaa atccaaaagc
    37921 ttatgatgta gctgaaagat atatcaataa aatagaatct gttgttgaag aaaaggggat
    37981 tggtttattt ctttattcaa taccaaacac agaaaatact tttggaacag gaacaggcaa
    38041 aacaacaaca gctgttgcaa tattaaatga atatgttctt aatgctacaa gaaggcactt
    38101 aaaaggacaa aaagagctga agaaaaatcc agcattattc attaaagctt ctgaactaca
    38161 aaacaaatat aatgcacagt ttagaggaac atttgaaatg caacaggaag cttcaaattg
    38221 cttctatagg cttaaagaaa gaatgaaggg tgtttcttta ttggttattg atgatattgc
    38281 tgtcagggac attacagaag cttttaaaaa tgaattgttt gaaatattgg attatagagc
    38341 aacagaaagt aaggcaacaa tattcacttc aaattatcca ataacaaaac ttgttgaatt
    38401 tttaggtgaa agaattgtat caagaattga tggaatgaca attcaagttg gcttccaagg
    38461 aaaagatcat agaaaagggg gcatattcta atggcaaata tagaaagtaa attattatca
    38521 aaagttgttg atgaaaagga cattactgtt ttagggaaat ataatataac agctaatgat
    38581 ttcacaacac aggctgaaac atttaatttc ataaaaagat atattaagga gtttggagaa
    38641 gttccagcct atacagaagt agttgctgaa tgtagttcat ttgaatatgt tcctgaagtt
    38701 cctgacaata tggcttatat gtgtaagaag ataaaatcag ataatgcaaa aagaagaatc
    38761 tttgaactac tacaaaaaga agcttcagaa aagttcagct ctatgacagg gacagcattt
    38821 gttgagtggt tacataaaga aacttcaaaa attaaagaag tagcttgtgt tgattctcat
    38881 gctggaagta actttgctac caatggggca gaaagaaaag caatgtatct tgaagctaag
    38941 gaaattggtt cagggcttta catagaaaca ccttattcaa agcttacaag atggcttaat
    39001 gggggctttg aacttggtga ttatgtatta ttacaagcat atacaaacag ggggaaatca
    39061 tggattgctt cagatattgg aatatgttca tggagaagtg ggaatggggt tcttcactat
    39121 tcacctgaat taagccaaaa gcaacagctt caaagactag acactttaaa tggtcacttt
    39181 agaaattcag aaataaagaa aggtcagcta tcaggaaagg ctgaagaaca atatcttaaa
    39241 taccttgata gcttcaatga aaacaatgaa accccatata ttataaaaac tatgggtgat
    39301 ttaccaaatg ggttgtcagt tgatgttata gaagcagacc ttcaagcaaa tgaaaatata
    39361 aaaatggtaa taatagatgg ctttaaccta atgaaccata aaggaaatga tggaaacaga
    39421 aacaacatga caaatacctc aagaagatta agacagcttt ttggaaagta taaggttgtt
    39481 ggtgttgtag tccatcaagt tcctacttca gcagaaaagg aaaatagaag tgaagatgaa
    39541 acaggggcaa gaatggttga gccaccaagg attgaccaat attcagaaac tgttgctgta
    39601 attcaagatg cctgtactat attaaacttt gaccaagctg atggtgttgg gaagataaga
    39661 cttgcaaagg ctagaacacc acatgttgat gaaatacttg aattacaatg tgattttgac
    39721 cttggatata tcaaggaagt atcagcaata gattttatat aggagtgaag aacatgacta
    39781 gagaagaaag taatttatta ttcaagcatt taaatagaaa gaaatacatt gaagcaatgg
    39841 aagaaacaca gtttgggtgg aagtcagcag aaaacttccc aaatgtgtgc tgtccttcag
    39901 aattagaatt cattagtgtt gaagaagaac catacaaagg ggaatgtcac acaacttgtg
    39961 aacaatgctg gaagtatgta cttgaaaata aaaattggta ggtgattcca tgaagatggg
    40021 ttttgaagaa aaaaggaaaa tattcattga cctgatgaaa aggaatgaaa gattaatgaa
    40081 agcactaaaa gaatatgatg aagaataggc tggtaaaatc cagcttattt tttttgtcca
    40141 aaaatgtcac aaagcttctg tgactattaa tttatatatt gtaacaaata aatcaaaaga
    40201 aaagaggaat taaaaatgaa tagacaatta caaataatag accaaaggga agttttagga
    40261 aaggacttca aaatatatgg aactaaagaa gaacctttat tccttgcaaa agatgttgca
    40321 aattggattg aacacaggaa tataactcac atgatgaaaa cagttgatga agatgaaaag
    40381 ctgacttaca caattagtaa ttcaggtcaa ggaagggaaa tgtggttttt aacagaagat
    40441 ggactttatg aagtattaat gcaaagtaga aagccaatag caaaagcatt taaaaaagaa
    40501 gttaagcaga tattaaaaca aataagaatg acagggggat atattcctgt taaggaagaa
    40561 gaaacagctg aacaattaat ggcaagggct ttaatggttg cacagaaaac attggagcaa
    40621 aaggatttaa tactgaaaca acaacaagaa aaacttcagg agcaaaagcc aaaggttgaa
    40681 tattatgaag aagtgctgga ttcatcaagg ttgattactg ttggacaaat tgcaaatgat
    40741 ttaggaataa caccacagaa attgaatcaa tatcttaaat taaaggggat tcaaaataat
    40801 caatataaaa cagggtggaa gatttcagca aaatataatt atctaattga agaaggatat
    40861 gctgattatc atatatatac aaaacattca gtacaggcac aacagcttaa atggacagaa
    40921 aaagggagaa agttcatcat tgacatgttt aatgaagata aaagaatttt tgagaacagg
    40981 aaataatcct gttctttttt ttatgtttat tttgaaagat aactttcaat aaaaatggaa
    41041 atgtcacaat taagaaacag cccttaattt aatcaatgaa tttagaaaat gaagggactg
    41101 aatgaaatgg aaaaattaaa ggtaaatgga cacttaattg atgtggatta tccagcagaa
    41161 cttgaattgt atatggatag gctggaaaag gtaagaatca ggggtgaaaa ggttcaagca
    41221 tgttcacctt ttagatatga acaacaccca agttgggctg ttaatttaga taatggaagt
    41281 tgggttgata gtggagcaac aaaagaaggt gaaagaaaag gaaattttgt caccttatta
    41341 gcacatttta ggggtgaaac ttatgaagaa acagctgaat atttacttga aaaatatgca
    41401 cattttcttg atgatgctga aagtctaaag ttgaatttaa acctgaaatt aaatgagcct
    41461 gaagttactg ttcttagtca agaaaagtat gctgatgttg ttgacaaacc ttccaattat
    41521 cttttgagaa ggggtatatc agttaaaata caaaaatatt tccaaacagg aaggggaagc
    41581 aagggggatt gtgttgcaat accttggcat gataaggctg gaagaataat caatattaaa
    41641 tacagaagta cacagggtaa agaattttgg ttcagtaaag gtggacagcc tataaaacag
    41701 catgtatatg ggttgtttgc tgttttaaat ctaaaggtca aagaagtttg ggctgttgaa
    41761 tcagaaattg atgctttata tctttggtca atggggtttc cagcaattgc ttttgggggt
    41821 gcatcaatga atgatacaca gaagaaatta cttcttaatt caggaataga aaccctagta
    41881 attgcaactg acaatgatgt tgtaggtaaa aggtttggtc aagtattaaa agaagaattt
    41941 gctggaattt taaatcttaa aaaaattgta tttcctgttg gggtgaaaga tgtgaatgat
    42001 cttaaccctg aagaattaat gaattgtgct aacaacttaa atataattaa agatttcata
    42061 aaattagtat gaaattagta tgaaattagc taatggttat ggggaatagg gtaaattctt
    42121 gttggatttg ttacactaat gtggtacaat atatgtgtaa aaaacagttc cacaaaagag
    42181 gaacaaagtc agggaagatt tgagaactat tttttaatag aatcaaaagg ggagagtaga
    42241 cataatagac ttatttcaaa aggtatatac attttaaata cttttagaaa tagggtttat
    42301 tatgtctatt tttttgtttt gatgctttat tttgcctttt tgtgttccac ttttatgcaa
    42361 caaataagat gttattttaa aaaattacta tataaaaatt tactgaaatg tggtacagga
    42421 ttactttaaa aatataaaaa tttaataatt aaaaaattta aaaaaattaa aagaaacagg
    42481 tcacatcttc ttctgacttt tgaatttatc taaaaaaaaa gacagtaagg agaagtgaaa
    42541 atgactaaaa aatacaaata tgaattaagt acaatatatg aaaaagtaaa tgaaatagta
    42601 aaagaaatcc aaaaagaaag cacttcagaa gtacaaaaga atttcttaat aggggactta
    42661 ttagaagtta cagaagaact tgttcaaaac aactgtaaga aatttatagc aaaatattct
    42721 ataaaagaca tatcacaaga agaattatat tcaattgcaa tatcacttcc attattagat
    42781 gcccttaaat ggttcagctt tgaaaaagga aataacttca tggcaacttg gaatgggttc
    42841 atgcaaaaaa gattcctaaa tgaattaaat gaattgagta atgataaaaa taaatggttc
    42901 agaaataatg tgtcaagtgc tgacaaagaa ctttcagaag atggaacaac aattattgat
    42961 atagttgggg aagaagactt cacagaagaa gtatgtgaag aaatggaatt aatgaatatt
    43021 attaaacaat ttgaagaagt ggacaagcat ggagctgtta taagatgttt ttttattgac
    43081 agtcaggagt atagaacaga agcaataaaa aaagctttgg gtgttaaaga atatggaagt
    43141 aaagaaagaa agcaagttca aagagctaag gaaagattta tcaaatatct aatacataat
    43201 aattatgatg tttcaaagta tttataaact acataaaaat aaaaagtaaa atataaagtt
    43261 tgtttagaaa gtcacaacaa tgtgactttt tttaatttat taaatgtaag gcaatgagcc
    43321 aagaaaaaaa agaaaaggag gattttatat gaaaaataac atgtttacag ctagaggaca
    43381 agaagcaaaa gcttcagcag aaaagggtaa agttgactta aagaaggtat atttaagaat
    43441 gaagtcaggt gattcaataa aagtaagggt tatgtcccca gctgattatg ttgaatatct
    43501 tgcacatagt tcatttactc acaaagtata tacacagcct tgtgttggag ttataggtca
    43561 agaatgtgct ttatgtaaag caagtaagtc aggcatagaa ggatttgatg ctctttatgc
    43621 taaaaaaaga tatttatttg tatttgctga tatgacttca ggtgaattaa gggttttaga
    43681 tgtttcaaaa aaccaagcta agaaattaat atcagatata gaagaataca aagaagattt
    43741 aaattcatta gctttcaact taaagaaggt tggagaagga acaaacacag gctatgcact
    43801 atcaccaata ataagaatga aagatacaga taaagcacaa tttgaagctt gtgaaggttt
    43861 agaagttaca gatgaatact taaatacagt attaatgcca agaacacttg agttacaagt
    43921 taagacatta aaagaagctg gattcccaac tgatgaatac ttcccacatg ttgatatttc
    43981 agataaagaa gaaggttcag aagatactgg aagaatagca gatgaagtgt tagacaatat
    44041 gtaattgaat aatatctttc aaaataggga tggctggaag gctgtccctt taaatttaag
    44101 gggggtatta tgaaatacaa atttgatata caagaaataa gcaaagaaag agctttggaa
    44161 atggtaacaa aattccatta ttcaaataca ttgccaactt taaacaaaat attcttggga
    44221 ttctttttgg aaggggaact tgttggaatg gtcacattag gttggggaac tagacccctt
    44281 cacacaatta agaagctatt tccttcatta gatacaaaag actattttga aattggaaga
    44341 atgtgcatga cagaagatat gccaagaaat tctgaatcac agatgctttc acaaatggtt
    44401 aaatggttaa aagtaaataa acctaaatta aaggtgattt tcacatgggc tgatgggatg
    44461 ctgggaaagg taggatatgt ttatcaagca tctaatttcc tatatgctgg acaagttgaa
    44521 tctgaaatat atttacataa tggagtgaag attcatccaa gacaaacaaa agctttattt
    44581 ggactagaag gggataaaag aaaaacagta agaccaagct gggagcagat gcaagagcat
    44641 gggataaaac actataaagg gaatcaattc agatatttgc tgtttttatg taacaagaag
    44701 gaaaagaaga agcttatgaa agaagcactt gttgacctat cttataacta tccaaaggaa
    44761 gatgctttaa gctggaaggt taaggttggt aatagagcat ggcagaagtg tccaccacct
    44821 aattatataa cagatagaaa attcaacaaa aatgaggact atttaacaga aaaaggtcac
    44881 aaagcctttg aaaaaactga atttattatt aggtatatat gaaaggaagg tggctgttaa
    44941 tgacacaatt atttaacagg ctaggagctg aaaggcttaa aaaaagagtt attgaaaatg
    45001 attcagaaag gggtcaaaga cttgctggtg ctttatataa tcagatgaat gatcttcaca
    45061 gcatagactt ttttcctgat tatgaaattg aaaagttatt gttggagcag aaggaacatg
    45121 acttggagtt aataaaaaat catcaaagat tacctgaagg actaattgta ttttcaccaa
    45181 gttcagcttc agcatgtaga aggggtttag tttttaaggc taataaagaa aagaaagatg
    45241 aagttttaag atacccctac caaagaagat ggacaagaaa tagttcagct gttcatgaag
    45301 ctgttcaaag ggatttactt tattgtgaaa aggtacttga taatcctatg tttactgttc
    45361 acagactaga aagtggactt ccagcttggg agcaaaatat caaaacacat aaaattattg
    45421 aacacaatgg agttaaattt tttctgaatg gaatgtgtga tggaatactt gaatataagc
    45481 ctgatggttc aaaaatatta tttgaattca aaacaaagtc aacaacaatt gggtgtgtag
    45541 gtacttataa gctgaagaat gttcaggaag aacatagaat acaagctgtt gcttattcac
    45601 ttctttttgg tgttgatgaa tgtatattca tgtatgaatc agttgctaaa gatggttgga
    45661 caaagggagc agatgcaaaa attgacttta gaacttttta tgtgaaggtt actgaagaag
    45721 ataaaaagga attacttgat aagttttcag aagtggcaaa agacttctat aacaacaaga
    45781 ttccatcaaa agaagataat aagtgcttct tttgtgttta taaggaagca tgtgaaaggg
    45841 ctggtgaata atatatgaaa agaatttatg ttcaagaagg aatctatata gttgtaaata
    45901 attcagggac tgttccaaag aagtgttttg aggtagcaaa aagaagaact tttccaacat
    45961 taccaagtgc aattaagttg ttcaagtttc ttcataggaa atatgattca gtttatatgt
    46021 accacaccaa agctgggttc atggtagaat tcaaaactta tgctgaatct ttaggcatag
    46081 ctcaagacat taaaaatgac atagaagcta ctttggaggt atttgaaaat gataagtaat
    46141 aaaagaattt tagcaatgga cttatcttta aatcttccag ccttctgtgt tgctgaagtt
    46201 gatgaagctg gtgaatttaa agtcatagaa gtttcacata tagataataa aaaagataag
    46261 aagctggaaa cagctgaaaa gcttcaaaag ttatatttac acttgaaaga tatttttcaa
    46321 aggtatggtg agtttgatgt tgtagtcagg gaaaaaggtt tcagcagatt tgcaaataca
    46381 acacaattat tatttagggt tgttggagta agtgatcttg ttgctcttca agaagctgga
    46441 atcaagtctg ttgaagaaat accaccaaca acaataaagt caattattgc tggatatagt
    46501 agagcagata aggaagaagt tgaagaaggt gttagaactt ttttatcaaa agaacagaag
    46561 aatatgaagt tctattctga tgatgaatca gattctgttg ctgttgcttt aacttatgca
    46621 atcaaaaagg ggtggatgaa atgataaaaa gaattaaaaa gcttcttgga cttcaaactt
    46681 gtgtacataa atgggagtgt gtaaatctac ttgaagcaaa taggggctac aagccaatat
    46741 tcaagtgtaa gaaatgtgga gaatatcaaa gataaaagaa aggtgctgaa aagcatcttt
    46801 tttttcttgt ttaaaatgtc acagaaaaac ttcaaaggtt aatttattat atgtaatcaa
    46861 taaaaaagaa aggaagtggc tgaatgaggg tcataaaaaa gaaaaaagtt gattgttatg
    46921 ttggtgatat agttttatac aaagattatc cttgcttaat agcagaagaa gaaagggaag
    46981 ctaatccata tattctgata tgccttgaag gtataaaagc tggacaagtt attggttctt
    47041 atgaaacact ttcaagaatt gatgatgtat gttctgaaat attaatcaag aaagataaag
    47101 taattatatc ttcagaagaa ggtgatgaat catgtccatt ttaacagtta atgttggagg
    47161 tatgttttca ggaaagtcaa cagaacttca aagacagggg gaaagacatg ttcttgctgg
    47221 tcataaggtt ttatttataa agcctgatat ggacacaaga tattctgatt gtgaggtggt
    47281 aacacataaa gggcagaaag ttaaagcaat aagtgttcca acatatgctg atttaaagaa
    47341 ttatataaat acagctgaag ttgatgtggt gttaattgat gaagttcagt tcttttcaga
    47401 aagaattatc ccttcaatat ggtggttatt atcaaaaggt atcagggttt atgcttcagg
    47461 gttagatatg gattttcttt ctgatggatt cccaacaaca gaaaaattga tggcaatggc
    47521 tgatgtagtt caaaagtttc atgctgtttg tgaaggttgt ggggaagatg ctgttatcac
    47581 agcaaaaaga aactttcatg aagtaggttc ttcagatgta attgaacttg gagcaaagga
    47641 aacctatatc ccattatgca gaagttgtta tataaagtat atggcaacta aaggggaaga
    47701 ttcaaatggt tagattagaa ttaaaatcaa tagaaaatta ttgtattcaa cagcagaaaa
    47761 gaattgctga gttgaaagtt attattcaaa acaaagacaa agaaattgaa agattaacta
    47821 aattgttaaa agacaaggaa agggacaggt gatttatggt gataacagtt ggtgcaatga
    47881 taggagcagg gaaaagttcc cttgcaaaat taataggtga acactttgga acagatgtat
    47941 tttatgaaag tgttgaagat aatccaatac ttccattatt ctacacagca agtgaagaag
    48001 aaattcaaga aaaaagatac ccatttttat tacaattata cttcttaaat actagattta
    48061 agagcataaa agaagcactt gttcatcaaa ataatgtgct tgatagaagt atatatgaag
    48121 attggtattt tgcaaaagta aatatggaac ttggaagaat atcagaatta gaattcaaaa
    48181 tttatgaaga attacttgaa aacatgttgg aagagcttga tgaactacca aagaaagctc
    48241 ctgatctaat ggtttattta tcaggaagtt ttgaaaccat acttgaaaga attaacaaaa
    48301 ggggaaggga ctatgaatta gcacctgaac ttgttgatta ttacagaaca ctttgggaag
    48361 ggtatgatga atggatgttc aaccattaca aagcttctga agtgcttgtg atagatattg
    48421 ataagtatga ctatgtaaac aatgaagaag atgcaaaaga agttcttaaa atgatagaag
    48481 ataaacttgc agaaataagg ggataaatat taaatttaaa agattattgt gtttaaccaa
    48541 ggaaggaagg ttgttatgaa aaaaaatata tttagcaagt ccttttttca atgtagttga
    48601 aaatgaaagg gtggataaag taaaagaaat attagattct aaagggcttg aggtattcag
    48661 tccaaaagaa catcaaaaca aacatttaga gtttgggaca atagagtgga gaaaggcaac
    48721 atttaagaat gatgttgatc atattgattg gtgtgattgt gttgtggcaa tcatatgcca
    48781 aggaaattat gatgattcag gaacagcatg ggaacttgga tatgcttatg ctacaaacaa
    48841 gcctgttata cttgtgaact taacaggaga agcaattaac ttaatgatag ctgacagttt
    48901 acatgcttta ataacaagtt atgatgaatt aaaggactat gatttcaatg aactaaaaag
    48961 aataccatat gaaaattatg tgtggtaaat aaaatcagaa agtggaggaa tttatagtgg
    49021 aactagcagt attttttaat ttattacaat taataggagg aataatcctt tcaataggat
    49081 atataccaca aatcagaaag actataagaa caaagtcagt tgaagattta tcaagtgctt
    49141 attatgtgaa tatatttatt ggtgttggtt gtatggaagc ttatgcagta tatagtgcat
    49201 tacatggtgt tgcttacatg ttcttagtta ccaatacaat agcatggtta cttgggggaa
    49261 caatgttatt cttaacttta aaatatagaa agagggttta atttatgaaa aaaattctta
    49321 tttttatact tgttataatc ttagccatta taatatgctt tgctgggtta gggcttataa
    49381 catttttctt aaataatttc ttagttgcta ttggtttaaa tgctgtgagc ataataaata
    49441 ttagtagttt actattggca attttattaa ttaaatggtt tttaaagact gaaagaaaaa
    49501 attgaaagat aattttcaaa aaggatagct gaaaagctgt tctttttttt atgtcacaaa
    49561 ttggtcacca actttgaatt tataaattga aatcaattga aagggtgtga cttgatgcaa
    49621 ttaaatttga atttagatat taataataca aaagaagcca aggctgaagc aagggtgaag
    49681 gaagccaaaa agaaaaaggc tttggcaaaa tatgaaccaa cttgggatga agtatgggtg
    49741 acaggatata aaacccatac aggagcaaca aagaaggcta tatttcaaac aaagttaact
    49801 gatatagata aggaaaaact tcttgctgtt aaggaagcaa ttgaaagtgg agaacttcaa
    49861 acaggggttg aatcattaag caagtttaca aaaacaaaag cccttggact gtgggttgtt
    49921 ctgaaggaaa ttagaaggga atcaataatt aaaaagatgg tagaagaaaa gcctgagaac
    49981 tatcacttaa tactgaatcc aaaggagctt catgaactaa caaataaact tgataaggaa
    50041 gaaatcattg ctgttgatac agaaacaaca ggggttgagg tttttggaaa agactatatt
    50101 gttggtatat ccttaacact tcctaatgct gattatcatt gttatatacc tataagacat
    50161 gttcatgaaa tagaattcat tgatgaacaa ggattcacag caacaaagta tgtaaggata
    50221 gaagaacagc tgaaagatac attggtgttt aatgtattaa aaccattctt ggagaatcca
    50281 aacctgaaga agatgttcca taactcaaag tttgatattc acatgttcag aaaagaaggt
    50341 gttgtgatta aagggctgta ccttgatact atggttgcaa tgcacttatt aaatgaaaat
    50401 gaaccaagct ttgcacttaa aaacttagca actaaatatg gaagatactt tggttttgaa
    50461 gataaatcaa tgacttatga agaacttttt gggaagggtg gctttgaaaa aacaccactt
    50521 gatattggaa cagtctatgc ttgtaaagat acccatttaa catataagtt tggttgctgg
    50581 atattagagc aatttgaaag acttccaaag ataaagaagt tatattttga aatagaatta
    50641 cctattacag aagtttgtat tgaaatggag aagaatgggt ttgcaataga ccttggcttt
    50701 gctgaacagt acaagctgga acttcaagaa gaaataaaag ggcttgaaga tagaatcagg
    50761 gaaggctttg gtgaaatcaa tataaacagt aaccaacagc ttcaaaaagt aatttatgaa
    50821 gaatggggtg ttcctgatga ttttaaaaca aaggtagatg ccccaacatt aaagcaaatt
    50881 gtttcaagat ataagaaaga tataccaaag gttgaatata taaatgacct tttaaaatat
    50941 agggaattga acaagcttct tacaacttat atagagccat taccacagaa gataagtcca
    51001 attgatggaa ggcttcatgg ttcatttaat caaagtgcaa cagtaacagg aagatttgct
    51061 tcaaataatc caaaccttca aaacttacca tacccagcaa gaaagatgtt tgttgcccct
    51121 gaagggaagc tgataattgg ttctgacttt tcacagatag aaccaagatt cctttcacat
    51181 atatcacaag atgctgactt catgggggca tatttagagg gaagggactt atattctgaa
    51241 atagcaagta aaactttcaa agttcctatt gaagaatgtt gtgatggaag catatggaga
    51301 aagaagagta aagtggtgct gttaggttgc atgtatggga ttagtgcaat gtcacttgct
    51361 gaacagttta acttaaccct tgctgaagct gaacaaatat atacagactt ttatggagca
    51421 tacccaacaa tgacagcatg gtttaaggct actaatgaca aggcaaatga acttgggtat
    51481 gttgaaacaa ctatgggaag gaaaagaaga ttccttggtc ataaggatat agcaagaaag
    51541 tattttgcaa tacataagaa catgcaaaga aaacttaaaa aagaatcatt taatatatgg
    51601 gaagaataca gaaagaaaaa agtgacttat gctgaagcta gagcctatca agatgttgct
    51661 ggacagttta acagggttca aagacagtca ataaatgctg ttatccaagg aagttcagca
    51721 gatattatga aaaaagcaat ggttgatata tacaggcatt tacagaagaa gggttctgaa
    51781 tggaagctgt taggaacaat tcatgatgaa gttcttgttg aaataccagc aacagctact
    51841 attgaagaaa tacaagaaat tgcagatata caaagaaatg ctgtaaaatt aagtgtacct
    51901 atgaaatgtg atgttgctat aatgaaaaga tggggtatag atataagttt acaagaatat
    51961 gcagaaggaa aagaagttcc ttgttaaaaa tatataagtc agtttaggac aaggtaaata
    52021 ccttgttctt tttttttatg tcacaatgtc tattctgtac ttaatttaaa ttttagaatt
    52081 acaacaaagg aataaatgaa taaaaaaatg attttaaaat taaaaaaaat aacacagaag
    52141 gtgatgtggg aagaattcac cagttgttg
//
LOCUS       DAEVGI010000006        42037 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEVGI010000006 DAEVGI010000000
VERSION     DAEVGI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 42037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42037
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.006"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            25..180
                     /locus_tag="K8M77_000347"
     CDS             25..180
                     /locus_tag="K8M77_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084876.1"
                     /translation="MKNALKLTFIGFLIILVGIYLAVSDIDTYGVHKVLTIIGVILAG
                     FGIKSIK"
     gene            337..666
                     /locus_tag="K8M77_000348"
     CDS             337..666
                     /locus_tag="K8M77_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBI7084877.1"
                     /translation="MKLVISIVQDDDALDLVDALNEKGYSVTKLATTGGFLKSGNTTL
                     LAGVQKEKVDDVISIIKDICETRKGVVSSSASVSEPGGVYIPYPIEVQVGGATIFVVD
                     VDKFIKI"
     gene            688..1611
                     /locus_tag="K8M77_000349"
     CDS             688..1611
                     /locus_tag="K8M77_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBI7084878.1"
                     /translation="MIGHNKVRDRFSKSVSKNALSHAHLIIGEDGIGKSLLADEFAYK
                     ILGIPSQREHVDIVHYKSEKASFGVDLVRNIIAEANKKPYECDRKVIIIHNGEKLTVA
                     AQNALLKTIEEPLTGIFIIILSSNIDSILETIRSRCQIHRLAPLSKEEINLYISKKYN
                     GIDDELNRTLISFSEGIPGRVDKFMNDDIFKDIRNTVIEMLKDIGHGKIEVPIKYSDK
                     FYSMKGNEEEILETITIFARDSIIFKEIENNLSIINGDKIDDIREIASNLSYNKLNRI
                     IKVVNEARNNLKSNTNLWLTLDSMLISILEE"
     gene            1614..2519
                     /locus_tag="K8M77_000350"
     CDS             1614..2519
                     /locus_tag="K8M77_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBI7084879.1"
                     /translation="MIEVIGVRFKKAGKIYYFGPNGIDIKKGQNVIVETARGIEFGEC
                     VIGIRKISEEDIVAPLKSVLRLATEEDINKHKENKAKETEAFEICLKKIEEHNLVMKL
                     IDVEYTFDNNKVIFYFTAEGRVDFRELVKDLATIFKTRIELRQIGVRDEAKMIGGLGP
                     CGRPLCCSTFLGDFASVSIKMAKEQSLSLNPTKISGICGRLMCCLNYEQSTYEEIRKE
                     LPRVGSVVETELGKGEVVGNNVLKELVRVKLPRKNEEIIQDFKIYDVKLISGSYEGEV
                     EDTNIRLEITDEADKALIKNLIKDN"
     gene            2688..2858
                     /locus_tag="K8M77_000351"
     CDS             2688..2858
                     /locus_tag="K8M77_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003363046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI7084880.1"
                     /translation="MAYKILDTCVSCGACAAECPVDAISQGDTQFVIDADTCIDCGNC
                     ANVCPVGAPVQE"
     gene            complement(2973..3626)
                     /locus_tag="K8M77_000352"
     CDS             complement(2973..3626)
                     /locus_tag="K8M77_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI7084881.1"
                     /translation="MNIYWLGHSCFLIKTNLGKRILMDPFDIDIGYIPYKGDVDLVTI
                     SHMHFDHNCTKYINDEAKVLTDLVDYQNTFCKVKSYLSFHDDCFGLKRDKNFIFKIIC
                     DDITLCHLGDLGHILSSKVIKQLGHIDILFVPVGEHLTISVENLKKVILDINPGYVIP
                     MHYKTAKLSFYLNTVDKFLMEMKDFKITHDQVLSINSESIIENTTIKILDLTSEDMI"
     gene            3843..4298
                     /locus_tag="K8M77_000353"
     CDS             3843..4298
                     /locus_tag="K8M77_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBI7084882.1"
                     /translation="MAKLSDSIEYFLKDLLDKTEDNNLTIRRNELADKFKCAPSQINY
                     VLTTRFTYEKGYLIESRRGGGGYIVIKKVIHEVHADRRDLIYDEIGTGITYHNAAAIV
                     DNLLEHNIIDEKTANITKVAINDRTLQSSKNINETRADIFKALLMVLLK"
     gene            4318..4845
                     /locus_tag="K8M77_000354"
     CDS             4318..4845
                     /locus_tag="K8M77_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084883.1"
                     /translation="MICESCNKNNATIQLVKSTKGKKERLMLCDKCAIEIMSLALEDE
                     EINLEDFLLDLNTYIECIEERVKDKALVCKNCGTRFSDFEENNFLGCEKCYEVFKNRI
                     IFLLNLEEAEIKHRGKQPKTMKKDLKKLEILNLQEKLKINILKEEYEEAIITKKKIHD
                     LKESIREDSNSGSMD"
     gene            4829..5842
                     /locus_tag="K8M77_000355"
     CDS             4829..5842
                     /locus_tag="K8M77_000355"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBI7084884.1"
                     /translation="MVQWISPLDNSIVIASKVKILRNIKGIKFTKLLNEDEFNDLLSM
                     VLGCFKEIDILDKCYVVKLKDGEEKIIDYYKENFGLIKYFENKDNLIFLMNKNGEFNI
                     LLNEEEHIGIECTNSGLSLREVYSKIDNLDDLIEEKIHYSFDSELGYLTSNIKNLGTA
                     LRAKVFIHLPLLSSNNLIKIIKNALKEEGITLKSIYNSGNKDVGNIYEVSNIKTLGMS
                     EKDILDSIISITNKLILREKNQRDNLSKDEYIELKDYILRALGILRNTYSIDRDEALK
                     YLSYVRLGVELGIIEKLTLKSVNSAMIEIQPDMINNSSIKKRDIQSLKIERAKIIRNA
                     LNT"
     gene            5867..8311
                     /locus_tag="K8M77_000356"
     CDS             5867..8311
                     /locus_tag="K8M77_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBI7084885.1"
                     /translation="MRLDNFNENSKIVINKGKESSLMLKHGYMGTEHLLIGLINENGQ
                     CSKILEESNVTEEKVLSFIKLYVGIGEVNYSLDEVPFTPRCKKILDRSVEIAKEYGHS
                     ISRPEHILLALIKEKEGVANLILTKIVKNDIKNISDKLNNILGRIPSKESKSLKEKEK
                     PKRKDTPTLNLYGINLIEKADKNKLDPVIGRKDETQRLLEILCRRMKNNPCLIGEPGV
                     GKTAVVEGLAQRILLGEVPDIIKDKRIFTLDVTSMLAGAKYRGEFEERLKKVLKEVKD
                     SDDIILFIDEMHNIVRAGGAEGAIDASNILKPALARGEIQCIGATTTDEYRKNIEKDS
                     ALERRFQPVVVKEPSKEDSVLILKGLREKYEVHHNVKLTDEAIEAAVNLSSRYITDRF
                     LPDKAIDLIDEAAAKVRIESMIVPPRLFKQEELIANLKIEKEEAIKLQDFELAAKLRD
                     EESMLQEELERDKELWREENYNKIYEVNKEHIAKVVSKWTQIPVERLTEKESSKLLKL
                     EEKLSKRVIGQLEAIKTISKAVRRSRVGLKDPKRPIGSFAFLGPTGVGKTELCKALAE
                     AMFGDENKFIRLDMSEYMEKHGVSRLIGAPPGYVGYEEGGQLTEKVRRNPYSVILFDE
                     IEKAHPDIFNILLQILEDGVLTDGKGKTVDFKNTIIIMTSNIGADKLDKKNAVGFSAK
                     EDKERDYDKMKGIMLEELKQNFKPEFVNRLDDIVVFHSLEKDHLLKIVDLMICSLKER
                     LNDLQLSLNFSQECKEFLVKKGTELKYGARPLRRVITKYIEDRLSEELLSGNILRGSK
                     VDVFLEDEVVKFKKTV"
     gene            8586..9524
                     /gene="trxB"
                     /locus_tag="K8M77_000357"
     CDS             8586..9524
                     /gene="trxB"
                     /locus_tag="K8M77_000357"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBI7084886.1"
                     /translation="MDKEIKELDLIIIGAGPAGLTSAIYASRAKLSTLVLEDNLVGGQ
                     VRSTYTVENYPGFTEITGNDLADRIQAQAEACGAIIDEFDFIENVSLKDDEKIIETGD
                     YIYKPKAVIIATGATPKKLPIPSESKYLGKGVHYCAVCDGAVYQDEVVAVVGGGNAAL
                     EEALYLSNIVKKVIVIRRYDYFRAEAKTLEAASNKENIEIMYNWDLVDVLGGEFVEAA
                     RIKNTKTGEEKEIAINGVFGYIGTEPKTSMFREYINVKENGYIEGDENMRTNVKGVYV
                     AGDVREKMFRQITTAVSDGTIAALHAEKYISEIKER"
     gene            9527..9760
                     /locus_tag="K8M77_000358"
     CDS             9527..9760
                     /locus_tag="K8M77_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HBI7084887.1"
                     /translation="MVKVYSVEWCGPCNKLKKYLEKLGVQYETITVADGKEDRNEVLE
                     VSGQRSVPVAVIGEEVIVGFDKQKIDAAIEKIR"
     gene            9777..11225
                     /locus_tag="K8M77_000359"
     CDS             9777..11225
                     /locus_tag="K8M77_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBI7084888.1"
                     /translation="MNTKENIFYNFMGGNFYENKSDKLIDIKSPLDGSLVGRIQSLSK
                     EDVDKIIDNAEKAQKSWNEVPLNERAHVLYKTADLLEEHKEELANIMIREVAKDKKSA
                     LSEVSRTADYIRFTADAAKSMDGESIPSDNFPGFNRSKISVVTREPLGVVLAISPFNY
                     PVNLSASKIAPAIVAGNSVVLKPATQGSLSALFLAKMFHDAGLPGGVLNTVTGRGNEI
                     GDYCVTHDKIDFINFTGSTEIGIRISEITVMKPLLMELGGKDAAIVLEDADLDLTVNN
                     IIAGGYSYSGQRCTAVKRILVMNSVADKLVEKLKEKISKLKMGNPLKVEDVTIVPLID
                     NKAADFVMELIDDAKEKGATLVCGGYREGNLIEATLFDNVTTDMRLAWEEPFGPVLPI
                     IRVNDKDEAIKIANESKYGLQSSVFTRDINEAFYIANKLEVGTVHVNNKTERGPDHFP
                     FLGVKSSGMGTQGIRYSIEAMSRPKATVITIR"
     gene            complement(11348..11575)
                     /locus_tag="K8M77_000360"
     CDS             complement(11348..11575)
                     /locus_tag="K8M77_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HBI7084889.1"
                     /translation="MVKVYSTNSCPWCVKAKQYLKSKNVEFEDLNVQTDMVAREDMLK
                     KSKQMGVPVLDINGTIIVGFDKNAIDKALSE"
     gene            complement(11669..11833)
                     /locus_tag="K8M77_000361"
     CDS             complement(11669..11833)
                     /locus_tag="K8M77_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084890.1"
                     /translation="MNLNDKFSNNYKVYENPLHSSNTALTSLSSFINNNEDAMHNLIF
                     NDHLNDDQIK"
     gene            12033..12755
                     /locus_tag="K8M77_000362"
     CDS             12033..12755
                     /locus_tag="K8M77_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7084891.1"
                     /translation="MIDKNSPIPVYYQLKEMIKEKIVDGTWQVGQCIASERELTEAYG
                     VSRMTVRQALGELVQEGLLVREKGKGTFVCEPKVKQEDMMSFSEIIKKAGRSLITKVI
                     DFEVINTPEELADTFDLEKIYKINRLRLVDGEAVANEVVYIPCEYCGFVTRDDLEGSL
                     FKLLEEKGHIIESTESSLAAVIMDDELKALFNVNKQVPLLKSHNTALNTEGEIIYVED
                     AIYRSDKYILEINISKREGKLK"
     gene            12752..13480
                     /gene="nagB"
                     /locus_tag="K8M77_000363"
     CDS             12752..13480
                     /gene="nagB"
                     /locus_tag="K8M77_000363"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI7084892.1"
                     /translation="MRLIVTKNYEEMSKVAAKEMAEDIKRNPEIVLGLATGGTPVGMY
                     KELIRMYNEGELDFSKVTSINLDEYVGLSGDHDQSYRYFMNTNLFDHINIDKNNTFVP
                     NGLAENIEEECMAYDARIQDIGGIDLQLLGLGANGHIGFNEPGEALSVGTHLTDLKES
                     TIEANARFFDSIDDMPRKAITMGLGGIMKAKKIMVIASGEGKAEVVKAMMSGKITTEI
                     PATMLQMHRDVILIVDEDAAKLLK"
     gene            13725..15086
                     /gene="radA"
                     /locus_tag="K8M77_000364"
     CDS             13725..15086
                     /gene="radA"
                     /locus_tag="K8M77_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBI7084893.1"
                     /translation="MAKVRSIYVCQNCGYETPKWMGKCPECNNWNTLVEEIRDAKSNQ
                     SSPKVERQIGELKKIKEIKSGEKERYDTGIGELNRVLGGGLVKGSLTLISGDPGIGKS
                     TLLLQTANNISKKYGKVLYVSGEESEEQIKIRGDRLKVDAEELYIVSETNLDVIEAYI
                     DKLEPAFIIIDSIQTIYRETVSSAPGSVSQVKECSNAVMRIAKGKNIPLFIVAHVTKQ
                     GDLAGPRVLEHMVDTVLSFEGERTEEFRILRTMKNRFGTTAEIGVFEMRGEGLIQVYD
                     PSSMFLEDTSFNQEGSVVIGVMEGTRPILVEIQSLASETKAVMPRRTSVGVENSRLSL
                     ILAVLEKKLRVPFYNTDVYVNVVGGLEIEGTTADLGIAISLVSSVKGKAARLEKLVVV
                     GEVGLTGEIRPISNCDRILNEAEKMGFSNAVVPYRSLEKLKGSKLNLIGVKTVREAIG
                     KIF"
     gene            15106..16170
                     /gene="disA"
                     /locus_tag="K8M77_000365"
     CDS             15106..16170
                     /gene="disA"
                     /locus_tag="K8M77_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBI7084894.1"
                     /translation="MRTVHDDELKKILKIMSPGTSLREGLDNILRAKTGGLIVLGDNE
                     EILDLVDGGFNINSEYSPAYIYELAKMDGALVLTSDRKRILYANAQLMPNQSISTFET
                     GTRHRTAQRVAKQTNKIAIAISQRRNIITVYKGDIKYVLRDSAVILSKANQAIQTLEK
                     YVAVLERVTNNLNILEFQDLATLFDVTTAIQRTEMVMRIVEEIEGYIIELGNEGRLIS
                     MQLNELVRSIEQDGVLLIRDYCYDKMEYNDVYKEIQELSAEDLLDLDIIAKELGYVGK
                     SLIDTLVSPRGYRISSKIPRIPSNVIENLVGHFGKLKYILEAGNEELDQVEGIGEARA
                     RAIKNGLRRIREQVALNKNL"
     gene            complement(16204..16605)
                     /locus_tag="K8M77_000366"
     CDS             complement(16204..16605)
                     /locus_tag="K8M77_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HBI7084895.1"
                     /translation="MKDVIFDDFQNSVNNSLLRHKSILDILSKYQESQARASRAICKA
                     VTNCGCIEISAKKQNMLEDTDLSLEELNPRLNSHIKGDLCENCREVIERELGNNLFYL
                     TSLCNALDLNLFDILLKEYNKMDTLGKFTFR"
     gene            16917..18023
                     /locus_tag="K8M77_000367"
     CDS             16917..18023
                     /locus_tag="K8M77_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBI7084896.1"
                     /translation="MIKKIFRVIFTILGLLIGFVLADGIRKSAIGNEYLTNIFLKVSF
                     YVILIAVFGIILFILSPFIYKSIRKLITFIEKGISKLSPSEIIFGAGGAIVALVLALV
                     ITLPLSSFLSGLNEMLGPIVSVIINIIAMVIGADIAVKKREDITGYFENFRKNQSKEK
                     KTKNSSNKKNEATPKVLDTSVIIDGRIFDLCQTGFIEGALVIPTFVLDELRHISDCSD
                     SLKRTRGRRGLDILNKIQKELPIEVIMWEGDFPEIAEVDIKLLKLAQKLNGKVVTNDY
                     NLNKVAEFQGVPVLNINELANAIKPVVLPGEEMTLTVVKDGKESGQGVAYLDDGTMIV
                     VEGGRKYMGKEITVVVTSVLQTAAGRMIFAKYKS"
     gene            18037..18714
                     /gene="ispD"
                     /locus_tag="K8M77_000368"
     CDS             18037..18714
                     /gene="ispD"
                     /locus_tag="K8M77_000368"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBI7084897.1"
                     /translation="MGKVVSVILAGGKGKRMGAEVSKQFIEINGKPIIYYTLKAFEEC
                     KDIDEIILVLPKDEIDYFKREIEPRFDFKISKIIEGGKERQDSVYNALNSIGDCDIVL
                     IHDGARAFVSNKIIEDGIKYSREFGAAAPGVMPKDTIKVKNLEGFSVDTPNRASLVAV
                     QTPQCFKYNLIKKGHNKVKNEKIQVTDDTMIVELLGEKVYLFEGDYKNIKVTTPEDLI
                     LAEHFVK"
     gene            19068..20780
                     /locus_tag="K8M77_000369"
     CDS             19068..20780
                     /locus_tag="K8M77_000369"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBI7084898.1"
                     /translation="MKMSNMLVGTLREVPAEAEIESHKLMLRAGLMRKMAAGIYNYMP
                     LGLKVIENVKNIVREEMNNAGAQEFLASALIPAELWQESGRWDAYGAEMFRLKDRHNR
                     DFCLGPTHEEVFTDIVRNEIKSYKQLPLNLYQIQTKYRDERRPRFGVMRSREFIMKDG
                     YSFDKDQEGLDLAYEKMRKAYVNIFNRCGLDAKAVAADSGAIGGSGSAEFMVKSEVGE
                     DDVVFCTACDYAANIEKAPSTPEHAEKEELMEVEKVETPAVKSIEDLAKFFECSPKKI
                     AKTLIFQADDKVVAVVLRGDREANEVKIANAIGEVIELEMASEEAVKEATGAAVGFAG
                     PMGIKVDILLVDQEVANMYNFIIGANETDMHLKNVNYGRDFEGIVGDFRNVTIGEKCP
                     ECGKEITISRGTEVGHIFKLGTKYSESMGATFIDEDGKAKPFIMGCYGIGVTRTVASI
                     IEQHNDENGIIWPLEVAPYHVSVIPANVKNEEQATKAEEIYNELRKMGVEALLDDRKE
                     RAGVKFKDSELMGIPMRITVGKMIGEGQVEFKLRNGGEVETLSIEEVYNRVRKEFERE
                     NLSL"
     gene            20888..22285
                     /locus_tag="K8M77_000370"
     CDS             20888..22285
                     /locus_tag="K8M77_000370"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBI7084899.1"
                     /translation="MKVYNTLNKKKEEFIPLTPGEVKMYVCGPTVYNFFHIGNGRTFI
                     VFDTIRRYFEYRGFKVDFVQNFTDIDDKMIKKANEEGTTVKKIGDTYIKEYYQDADAL
                     NIERATVNPRATEFIGEIIKFVKGLVDKGYAYEVDGDVYFSTKKFEGYGKLSGQNIED
                     LQSGARISVDERKKDPMDFAIWKAQKPGEPAWNSPWGMGRPGWHIECSCMAKKLLGET
                     IDIHAGGSDLKFPHHENEIAQSEALTGEPFARYWLHSAFVNVNNEKMSKSLNNFFTAR
                     EILERYDADVIRFLMLSAHYRQQLNFSEDLLESAKASVERIYNAIGNLENLIDEVSRE
                     EMNEEEKAYLESLNKYKEKYIEKMDDDFNTADAITAIFDLIKDTNTNITIDSSKELAQ
                     KALELIRELGAPLGMFQKSTKGNLEEEIEALIAKRQQARKDRDFALADKIRDELKDRG
                     IVLEDTPQGVRWKRI"
     gene            22512..22922
                     /locus_tag="K8M77_000371"
     CDS             22512..22922
                     /locus_tag="K8M77_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBI7084900.1"
                     /translation="MKSLLYREFSESEARMLNPLQLALIGDGVYEVFIRNYILSENAG
                     LSAHKIHVKAIQYVKAKAQSDIINSLEGELREDELYIYKRGRNAKSATVPKNANVRDY
                     RNATGFEALVGYLYLTGKEERLNEFLLKCIEVHV"
     gene            22938..23675
                     /locus_tag="K8M77_000372"
     CDS             22938..23675
                     /locus_tag="K8M77_000372"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBI7084901.1"
                     /translation="MLKVKLLQYTPEPEKTIAAAAKLCYSPVGVDDILENLTDEGAEK
                     FLNMLMSYGHMSPIEHVSFTFAVEGVSRSLTHQLVRHRIASYSQQSQRYVKLDQFEYI
                     VPPAIENDEVAKKLFIDQMQDAQKAYDEIVERLKDKYISEGIGEKPAEKKAIEDARYV
                     FPNACETKIVFTMNARSLMNFFHHRCCDRAQWEIRTMAEKMVDEVKKVAPILFKNAGP
                     SCVAGPCPEGKMCCGKLKDMRTLYLGK"
     gene            23698..24567
                     /gene="rlmB"
                     /locus_tag="K8M77_000373"
     CDS             23698..24567
                     /gene="rlmB"
                     /locus_tag="K8M77_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBI7084902.1"
                     /translation="MTKSENKYKQLTRKQKYALEREQRAREEKYARNTRTFAEEEVKV
                     REDLVEGRNAVIELLKSDKTIEQIFIANGKMEGSINKILGLAKEKKIVVKEVDRKKLD
                     LMSETKAHQGVIAQITPFKYSTVEDILDLAKSKNEDPFIVILDELEDPHNLGSIVRTA
                     ELCGAHGIIIPKRRNVGVTGTVYKASVGAIEHMKIAKVTNINNAIEELKANNVWIYGA
                     DIAGEEYSYETNFKGACGIIIGSEGRGMSNLTKKKCDKLVKIPMVGKINSLNASVAGG
                     IMMYEVLKSRIIK"
     gene            24583..25092
                     /locus_tag="K8M77_000374"
     CDS             24583..25092
                     /locus_tag="K8M77_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBI7084903.1"
                     /translation="MRVIFIDGYNVINSWPNLKKSKNYSYDIARTELTEMLENYAAFN
                     SCKIYLVFDAHKVAGNIESKELSGNIITVFTKEGEIADAYIERMVDELGRKVEIVVVT
                     SDRLEQQTIFQRGAVRMSSIEFYNEVLKVQDNIRKKAEKNKLELGKNFLGDTLEESVL
                     EKLEKMRRG"
     gene            25174..25821
                     /gene="sigH"
                     /locus_tag="K8M77_000375"
     CDS             25174..25821
                     /gene="sigH"
                     /locus_tag="K8M77_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBI7084904.1"
                     /translation="MGITYDLAKINNYEEFKDLTDKEVVMLAKNGDNMAQEYVISKYE
                     QFVKAKSKSYFLIGADKEDIYQEGMIGLYKAIRDFNPDKFASFKSFAELCVTRQIITA
                     IKTATRQKHIPLNTYISLSKPIYEEESDRTLLDTVVGITITDPESLIIGKEEVEKIES
                     AINSVLSELELEVLNSYLDGKSYQEIACDLDREAKSIDNALQRVKRKLEKCLNIK"
     gene            25904..25979
                     /locus_tag="K8M77_000376"
     tRNA            25904..25979
                     /locus_tag="K8M77_000376"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25937..25939,aa:Thr,seq:ggt)
     gene            26046..27239
                     /gene="tuf"
                     /locus_tag="K8M77_000377"
     CDS             26046..27239
                     /gene="tuf"
                     /locus_tag="K8M77_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI7084905.1"
                     /translation="MSKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAQAGGAEA
                     FKYDEIDKAPEEKERGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLSSRVGVDHIVVFLNKADMVDDEELLELVEMEVRELLSE
                     YNFPGDDIPVIKGSALVALENPTDEAATACIRELMDAVDSYIPTPERATDKPFLMPVE
                     DVFTITGRGTVATGRVERGVLHVGDEVEVIGLTEERRKTVVTGIEMFRKLLDEAQAGD
                     NIGALLRGIQRTDIERGQVLAQVGTINPHKKFVGQVYVLKKEEGGRHTPFFDGYRPQF
                     YFRTTDVTGSIKLPEGMEMVMPGDHIDMEVELITEIAMDEGLRFAIREGGRTVGSGVV
                     TSIIE"
     gene            27422..27571
                     /gene="rpmG"
                     /locus_tag="K8M77_000378"
     CDS             27422..27571
                     /gene="rpmG"
                     /locus_tag="K8M77_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBI7084906.1"
                     /translation="MRVKVTLACTECKQRNYNTMKNKKNDPNRIEMKKYCKFCKTHTL
                     HRETK"
     gene            27625..27849
                     /gene="secE"
                     /locus_tag="K8M77_000379"
     CDS             27625..27849
                     /gene="secE"
                     /locus_tag="K8M77_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBI7084907.1"
                     /translation="MATKQNVNEKSRGFGITKFFRGVKAEIKRITWPPKEEAKKAIIA
                     VIVFTVISIALIGVMDFVFKNLFELVFGLK"
     gene            27887..28408
                     /gene="nusG"
                     /locus_tag="K8M77_000380"
     CDS             27887..28408
                     /gene="nusG"
                     /locus_tag="K8M77_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBI7084908.1"
                     /translation="MSDRARWYVVHTYSGYENKVKANLEKTIENRNLESLILDIQVPM
                     EESAEEKDGKIKVTLKKKFPGYVIVNMVMTDESWYVVRNTRGVTGFVGPGSKPVPLTD
                     EEVESMGVMKTAMDIDLEVGETINIISGALSGFAAIIQEINVEKGKIKALVDMFGRET
                     LAELDFNQVEKLD"
     gene            28483..28908
                     /gene="rplK"
                     /locus_tag="K8M77_000381"
     CDS             28483..28908
                     /gene="rplK"
                     /locus_tag="K8M77_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBI7084909.1"
                     /translation="MAKKVTGMIKLQLPAGKATPAPPVGPALGQHGVNIMGFCKEFNA
                     KTADKAGLIIPVVITVYQDRSFSFILKTPPAAVLIKKELGLESGSGVPNRTKVGNITK
                     EQIRKIAELKMPDLNAATIETAMSMIEGTARSMGVVVVE"
     gene            28966..29655
                     /gene="rplA"
                     /locus_tag="K8M77_000382"
     CDS             28966..29655
                     /gene="rplA"
                     /locus_tag="K8M77_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBI7084910.1"
                     /translation="MGKKYIESSKLIDKNALYTPAEALELAVKTAKAKFDETIELHVR
                     LGVDPRHADQQVRGAVVLPHGTGKDVKVLVFAKGEKAKEAEAAGADFVGADELVQKIQ
                     GENWFDYDVVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVANAIKEIKAGKVE
                     YRVDKTAIVHCPIGKKSFGTEKLVENFKALMDALVKAKPAAAKGQYLKSVSVSATMGP
                     GAKVNPAKVLD"
     misc_feature    29711..29837
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            29872..30372
                     /locus_tag="K8M77_000383"
     CDS             29872..30372
                     /locus_tag="K8M77_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBI7084911.1"
                     /translation="MNKNRQLKEAKVAEIKEKLEKAQSVVLASYQGLTVEQDTNLRKS
                     LREAGVEYKVYKNTLVTLAAKELGFDGIVEYLEGPVSIAFGYEDATAPARILNDFAKE
                     NKKLELKAAIVEGTVYNKEQTEQLAAIPSREVLLAKLLGSIKSPLSNFAYLLSAIADK
                     KEESAE"
     gene            30427..30792
                     /gene="rplL"
                     /locus_tag="K8M77_000384"
     CDS             30427..30792
                     /gene="rplL"
                     /locus_tag="K8M77_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBI7084912.1"
                     /translation="MTKEQIIEAIKEMSVLELNELVKACEEEFGVSAAAPVAVVGGAA
                     AGAAAEEKSEFDVVLTNAGANKIKVIKAVRELTGLGLKEAKEIVDGAPKTLKEAVAKE
                     EAEDMKAKLAEVGAEVELK"
     gene            31097..34801
                     /gene="rpoB"
                     /locus_tag="K8M77_000385"
     CDS             31097..34801
                     /gene="rpoB"
                     /locus_tag="K8M77_000385"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBI7084913.1"
                     /translation="MVHPVQVGKRTRMSFAKVKDVAEMPNLIEIQLDSYKWFLDAGLY
                     EVFDDINPISNFTGNLVLEFVGYTLDMDNIKYSVEECKERDTTYAAPLKVAVRLQNKE
                     TGEIKEQEVFMGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYYNYNVDKTGKKLFSA
                     TVIPNRGAWLEYETDSNDVIYVRIDKTRKLPISILGRAMGFGSDQELLEYFGEEERFK
                     ATIEKDNTKTKEEALLEIYKRLRPGEPPTVDSAISLIDSLFFDAKRYDLSRVGRYKFN
                     KKLAIGLRIANQIAAEDIVDKLTGEVLVAKGEKISRANAEEIQNRGINSVDVLVEDRV
                     IRIIGNHFVDIHKCVDFDISDLNIRELVHYPTLREILDNYSDEETIKEEIKKNMTRLI
                     PKHIIKDDIFATISYQIGLAYNIGYVDDIDHLGNRRLRSVGELLQNQFRIGLSRMERV
                     VKERMTIQDQEAITPQQLINIRPVAAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLATYAKVNEYGFIETP
                     YRVVDKSEGRVTGEIRYFTADEEDQYLVAQANEPLDENGCFIDKKVTVRDKGEVLVVP
                     SKDVDLMDVSPRQLVSVATAMIPFLENDDASRALMGSNMQRQAVPLLKPYAPIVGTGI
                     EYKAAVDSGVLPKAKNAGEVVYVSANEVRVKRELDGGVDTYRLLKFKRSNQGTCINQR
                     PIVAKGDWVLKGEVLADGPSTDLGEIALGKNIRMGFITWEGYNYEDAMLISEELVRED
                     VFTSIHIEEYECEARDTKLGPEEITRDIPNVSEDALKDIDERGIIRIGAEVRSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGEAGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAQKRKISVGDKMAGRHGNKGVISRVLPEEDMPFLPDGRPLQICLNP
                     LGVPSRMNIGQVLEVHLGWAASALGWHIATPVFDGATETDIEDCLEKAGYNRNGKTVL
                     RDGRTGEEFDNEVTVGIMYILKLAHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHTLQEILTVKSDDVVGRVKTYEAIVKGENIPEPGVPESFKVLIK
                     ELQALCLDVKVLNDNNQEVKFKELAEDDDEIEVLEVNMEGTEDSTTEEAKEEKGEAYI
                     PAEEIDEEIDYENIDLLDFTSDLDIEDDFN"
     gene            34881..38417
                     /gene="rpoC"
                     /locus_tag="K8M77_000386"
     CDS             34881..38417
                     /gene="rpoC"
                     /locus_tag="K8M77_000386"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBI7084914.1"
                     /translation="MFELNNFDALQIGLASPEQIREWSRGEVKKPETINYRTLKPERD
                     GLFCERIFGPIKDWECHCGKYKRVRYKGIVCDRCGVEVTKSKVRRERMAHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRALEKVLYFASYIVIDPKETSLLKKQLLNEKEYREACD
                     KYGEESFVAGMGAEAIKTLLSEIDLERTAAELKEELKQSTGQKKVRIIRRLEVVESFK
                     SSGNKPEWMVVDVIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLDLG
                     APDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLG
                     KRVDYSGRSVIVVGPELKMYQCGLPKEMALELFKPFVMKKLVEDGVAHNIKSAKRMVE
                     RVMPQVWDVLEEVIADHPVLLNRAPTLHRLGIQAFQPVLVEGRAIKLHPLVCTAYNAD
                     FDGDQMAVHVPLSVEAQAEARFLMLAAGNIMKPSDGRPVCVPTQDMVLGSYYLTMDKD
                     GAKGEGKYFASFDEVIMAYQLKEVDIHAKINVKVTKEIDGELKSGIIKTTPGFIIFNE
                     CIPQDLGFVNRENPEEMFNLEIDFLITKKSLGKIIDKCYLKHGPTKTSIMLDNIKATG
                     YHYSSIGAVTVAASDMIVPQKKYELLKEADETVDKIEKMYRRGLISEDERYERVIEKW
                     TETTEEVADTLMNSLDKFNPIFMMADSGARGSKSQIKQLAGMRGLMASPSGKIIELPI
                     RASFREGLDVLEYFISTHGARKGNADTALKTADSGYLTRRLVDVSQDVIVREHDCGTQ
                     NGIYVEEIKEGSEVVEQLAERLTGRYTAEDVFHPETGELLAAKDTYMDPILAEKIADT
                     GIQKVKIRSVFTCDSKVGVCTKCYGMNMATSYKINIGEAVGIVAAQSIGEPGTQLTMR
                     TFHTGGVAGADITQGLPRVEELFEARKPKGLAIVSEVAGTVRIEETKKKRTVYVVTDS
                     GEEYSYDIPFGSRLKVKDGIAIGAGDEITEGSVNPHDIMSIKGVDGAREYLLSEVQKV
                     YRLQGVDINDKHLEVVVRQMTRKIKVTEQGDTNLLPGVMIDMFDFRAENERVESFGGE
                     KAQGDIVLLGITKAALATDSFLSAASFQETTRVLTDAAIKGKIDPLVGLKENVIIGKL
                     IPAGTGMMKYRSLKLNTENSNQETETIIEIEE"
     gene            38530..38769
                     /locus_tag="K8M77_000387"
     CDS             38530..38769
                     /locus_tag="K8M77_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBI7084915.1"
                     /translation="MVDRLLGKKVIGIKQSTKAMKNGEGKILYVAKNANDKMVTPLVE
                     LAEKCGIEIKHVKNMKILGEMCGIDVKSAVALILD"
     gene            38827..39207
                     /locus_tag="K8M77_000388"
     CDS             38827..39207
                     /locus_tag="K8M77_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBI7084916.1"
                     /translation="MPTISQLVRKGRKTVASKSTAPALKECPQKRGVCTVVKTTTPKK
                     PNSALRKIARVRLTNGYEVTAYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGAL
                     DSAGVANRMQGRSKYGAKKPKQKK"
     gene            39367..39837
                     /gene="rpsG"
                     /locus_tag="K8M77_000389"
     CDS             39367..39837
                     /gene="rpsG"
                     /locus_tag="K8M77_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBI7084917.1"
                     /translation="MPRKGHIAKRDVLPDPVYNSKVVTKLINNVMEDGKKGVAQKICY
                     DAFQIINEKTGRDAMEVFEEAMNNIMPLLEVKARRIGGANYQVPIEVRPERRQTLGLR
                     WLLAASRKRGEKYMRERLAGELMDAANNTGAAVKKREDTHKMAEANKAFAHYRY"
     gene            39918..41984
                     /gene="fusA"
                     /locus_tag="K8M77_000390"
     CDS             39918..41984
                     /gene="fusA"
                     /locus_tag="K8M77_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBI7084918.1"
                     /translation="MARQYPLEKFRNFGIMAHIDAGKTTTTERILFYTGRNHKIGETH
                     DGASTMDWMAQEQERGITITSAATTCFWKGYELNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAKSGVEPQTETVWRQADKYGVPRMIYVNKMDATGADYYNCINTVRERLQANAV
                     AIQIPIGQEDQFQGMVDLLTNQAIIFKDDLGKDIEVGEVPADLADKAEEYRAAMIEAI
                     AETDEELMMKYLEGEELTLEELKVALRKATINNEIIPVICGSSYKNKGVQQMIDGVVD
                     YLPSPLDIPAVKGTNLDGEEEVREASDDAPMSALAFKIATDPFVGRLAFTRVYSGVLE
                     SGSYVLNSTKGKKERIGRLVKMHANSREEVESLEAAELGAVIGLKNTTTGDTLCTEAA
                     PIILEKMEFPEPVISIAIEPKTKAGQEKMGIALSKLAEEDPTFKTWTDQETGQTIIAG
                     MGELHLDIIVDRLQREFKVECNVGAPQVAYKETIKKAVEAEAKFARQSGGRGQYGHCK
                     IEMIPTEGEYEFENAIVGGAIPREYIPAVDNGIREAAESGIIAGYPVINFKIRLFDGS
                     YHDVDSSEMAFKIAGSMAFKNAMAKADPVLLEPIMKVEITVPEEYMGDVIGDVNSRRG
                     RMEGMDSRNGAQIIRAFIPLSEMFGYATALRSRTQGRGTYAMEFDHYDDVPKSIQEEV
                     AGKKNK"
BASE COUNT        16649 a         4877 c         8335 g        12176 t
ORIGIN      
        1 ctaaataatc tgggggaaaa taatatgaaa aatgcattaa agttaacttt tattggattt
       61 ctaataatat tagtaggaat atacttagct gtttcagata tagatacata tggagtacat
      121 aaagttctta ctataatagg agttatttta gctggttttg gtataaagag cataaaataa
      181 tttagctatt aaaaatatta attttaaata aaaagtaaat tatataactt atgaaattaa
      241 agcaataata aggtttggtt aagggttgta aataatgata taattattta aaacataaat
      301 ttgaatttta gggtagagat gtgaggagga cgtgttatga aattagtaat atctatagtt
      361 caggatgatg atgctttaga tttagttgat gcgttaaatg agaaaggtta tagtgtaact
      421 aaattagcta caacaggtgg atttttaaaa tcaggaaata ctacactttt agctggtgtt
      481 caaaaagaaa aggttgatga tgttataagt ataataaaag atatatgtga gacaagaaag
      541 ggagttgtaa gttcatctgc aagtgtttca gaacctggtg gagtgtatat accatatcct
      601 atagaagttc aggttggagg agctacaata tttgttgttg atgtagataa atttataaag
      661 atttaaaata gaaaggaaag tgcataaatg ataggtcaca ataaggttag agatagattt
      721 agtaaaagtg taagtaaaaa tgcactttcc catgctcatt taattattgg tgaggatgga
      781 ataggaaaaa gtcttttagc agatgaattt gcttataaga ttttaggaat accatcacag
      841 agagaacatg tagatattgt tcattataaa tctgaaaagg cctcttttgg tgttgattta
      901 gtaagaaata ttatagctga ggcaaataaa aaaccttatg aatgtgatag aaaggttata
      961 ataattcata atggagaaaa gcttacagtt gcagcacaga acgctttatt aaagaccata
     1021 gaagaacctc ttacaggtat ttttataata attttaagta gtaatattga ttctatatta
     1081 gaaactataa gatctagatg tcaaatacat aggttagcac ctcttagtaa agaggaaatt
     1141 aatttatata tatctaaaaa gtataatgga atagatgatg aattaaatag aactcttata
     1201 tcttttagtg aaggtatccc aggaagagtt gataaattca tgaatgatga tatttttaag
     1261 gatataagaa atacagttat agaaatgtta aaagatattg ggcatgggaa aatagaagtt
     1321 cctataaaat attctgataa attttatagc atgaagggta acgaagagga aatcttagag
     1381 accataacta tttttgccag agactcaatt atatttaagg aaatagaaaa taacttgtcc
     1441 ataataaatg gagataaaat agatgatatt agagaaatag ctagtaattt atcctataat
     1501 aaattaaata gaataataaa agtagttaat gaggccagaa ataatttaaa aagtaatact
     1561 aatttatggc taactttaga tagtatgctg attagtatat tggaggaata aaaatgatag
     1621 aagtaatagg agtcagattt aaaaaagctg gtaaaatata ttactttggt cctaatggta
     1681 tagatataaa gaagggtcaa aacgtaattg ttgaaactgc tagaggtatt gaatttggag
     1741 aatgtgtaat tggtataaga aaaattagtg aagaggatat cgttgcacca ttaaaatctg
     1801 tattaagatt agctacagag gaagatataa ataagcataa ggagaataaa gctaaagaga
     1861 cagaggcttt tgaaatatgc ttaaagaaga tagaagaaca taatttagta atgaaactaa
     1921 tagatgtaga atatacattt gataataata aggttatatt ctactttact gcagagggta
     1981 gagttgactt tagagaatta gtaaaagatt tagcaactat atttaagaca agaatagagc
     2041 ttagacaaat aggggttaga gatgaggcta aaatgatagg tggacttgga ccatgtggaa
     2101 gacctctttg ttgctctaca ttcttaggag actttgcttc agtttcaata aaaatggcta
     2161 aagaacagag cttatcacta aatccaacta agatatcagg aatatgtgga agacttatgt
     2221 gttgtttaaa ttatgagcaa agcacatatg aagaaataag aaaagaactt cctagagtag
     2281 gatctgtagt tgagactgaa cttggaaaag gtgaagtagt aggtaacaac gtattaaagg
     2341 aattagtaag agttaaatta ccaagaaaaa atgaagagat tatacaagat tttaaaattt
     2401 atgatgtaaa attaatctca ggtagctatg aaggtgaagt agaggataca aacattagat
     2461 tagaaattac tgatgaagcg gataaagcat taattaaaaa tctaataaaa gataattaaa
     2521 attaaatctt taatacggta taggggtatt ctttagcatg ttaattctaa ttttactaga
     2581 ataggctaaa tatgcttaaa agagatatta aatactttaa aaattagcaa aaatatgata
     2641 atataaatat ggtattttta taccaacatt ttaggaggtg ttttattatg gcatacaaaa
     2701 tattagacac atgtgtaagc tgtggagcat gcgctgctga gtgtcctgta gatgcaatat
     2761 cacaaggaga tactcaattc gttatagatg cagatacttg tatcgattgc ggaaactgcg
     2821 ctaatgtttg tccagtaggt gcaccagttc aagaataata ttattaaaga tcatcttcgg
     2881 atgatctttt tttcaatttt atgctaattt tcaattttaa aactatactg ttatataatt
     2941 ttaaaatatt ttgaggatta atttatatat tttcatatca tatcttcgga agttagatct
     3001 aagattttta ttgtagtatt ttcaataatt gactcagaat ttattgacaa aacctgatca
     3061 tgagttattt taaaatcttt catttccatt agaaatttat ctacagtatt taaatagaaa
     3121 cttaattttg cagttttata atgcattgga ataacgtaac caggatttat atctagaatt
     3181 acctttttta gattttctac agaaatagtt aaatgttctc ctacaggaac aaataatata
     3241 tctatatggc ctagttgttt tataacttta gaagaaagta tatgtcctaa atctcctaaa
     3301 tgacaaagtg ttatgtcatc acaaataatt ttaaatatga agtttttatc acgttttaat
     3361 ccaaagcaat catcatgaaa agataaatat gatttaactt tgcaaaaggt attttgataa
     3421 tcgactaagt ctgttaatac ttttgcttca tcattaatgt attttgtaca attatgatca
     3481 aagtgcatat gacttatagt aactaagtcc acgtcacctt tatagggaat atatcctata
     3541 tctatatcaa atggatccat taatatacgt tttccaagat tggtttttat taaaaaacaa
     3601 gaatgaccta accaataaat attcattaaa aagcctccta taaaaattat ttatattatt
     3661 gacacatata gtataaataa acataaaata taagaataat ttatttgaat tgaatttatt
     3721 taagggaatt taaataaaaa taataaatga ataattttat tacattgatt aagtatgtca
     3781 aatgtattaa tatatattgt gtattaaggt cagtaaaggt caaattttga ggagtgatat
     3841 atatggctaa attgtcagat tcaatagaat attttctaaa ggatttatta gataaaacag
     3901 aagataacaa tttaacgata agaagaaatg agttagctga taagtttaag tgtgcaccat
     3961 cacagataaa ttatgtttta acaactagat ttacctatga aaaaggttat ttaattgaaa
     4021 gtagaagagg tggtggtgga tacattgtaa taaaaaaagt aattcatgaa gttcatgctg
     4081 atagaagaga tttaatttat gatgagatag gcacagggat tacatatcat aatgcagctg
     4141 ccattgtaga taatttactt gaacataata taatagatga aaaaacagca aatattacta
     4201 aggtagcaat aaatgataga acccttcaga gttctaagaa tataaacgaa acaagagcag
     4261 atatttttaa ggctttgctt atggtattac ttaagtaatt tatgggagtt gaaaattttg
     4321 atttgtgaga gttgtaataa aaataatgca acaatacagt tagtaaaaag cactaaggga
     4381 aaaaaagaaa gattaatgct ttgtgataaa tgtgcaattg aaataatgag ccttgcacta
     4441 gaagatgagg agataaattt agaagatttt ttattagatt taaatactta tatagagtgc
     4501 attgaagaga gggttaagga taaagcttta gtatgtaaaa attgtgggac aagattttct
     4561 gattttgaag aaaataattt cttaggttgt gaaaaatgct atgaagtatt taaaaataga
     4621 ataatatttt tattaaattt agaagaagca gagataaaac atagagggaa acaacctaaa
     4681 acaatgaaaa aggatttaaa gaaattagaa atattaaatt tacaagaaaa attaaaaatt
     4741 aatatactga aagaggaata tgaagaagct attattacta agaaaaaaat acatgattta
     4801 aaagagagta taagggagga tagtaatagt ggttcaatgg attagcccct tagataatag
     4861 tatagtaatt gcatcaaaag ttaaaatctt aagaaacata aaaggaatta aattcactaa
     4921 gctattaaat gaagatgaat ttaatgatct tttaagtatg gttttaggat gctttaaaga
     4981 gattgatatt ttggataaat gttatgtagt taaattaaaa gatggtgaag aaaaaataat
     5041 agattactac aaagaaaatt ttggtttaat aaaatacttt gaaaataagg ataatcttat
     5101 ttttttaatg aataaaaatg gagaatttaa tatattactg aatgaagaag agcatattgg
     5161 gatagaatgt acaaatagtg gattaagttt aagagaagtg tattctaaga tagataattt
     5221 agatgactta attgaagaaa aaatacacta ttcctttgat tctgaattag gatacttaac
     5281 atcaaatata aaaaatttag gaacagcttt aagagcaaag gtatttatac acctacctct
     5341 tttaagttca aataatttaa taaaaataat aaaaaatgct ctaaaggaag aaggaattac
     5401 tctaaagagt atatacaatt caggaaataa agatgttggc aatatttatg aagtatctaa
     5461 tattaagact ttaggaatgt cagaaaaaga tattttagat tctattatat ctattactaa
     5521 taaattaatt ttaagagaga aaaaccaaag agataatctt tcaaaggatg agtatattga
     5581 attaaaagat tatattttaa gagccttagg aattttgaga aatacctatt caatagatag
     5641 ggatgaagct ttaaaatatt tatcttatgt aagattagga gttgaactag gaattataga
     5701 aaagcttact ttaaaatcag taaattctgc tatgattgaa attcaacctg atatgataaa
     5761 taactcatca attaaaaaga gagatataca aagtttaaaa atagaaagag ccaaaattat
     5821 tagaaatgct ttaaacacat aattataggg tattgggagg taaaagatgc gtcttgataa
     5881 ttttaatgaa aattccaaaa tagtaatcaa taagggaaaa gaaagttctt taatgctaaa
     5941 acacggctac atgggaacag agcatttatt aataggatta attaatgaaa atggacaatg
     6001 ttctaaaatt ttggaagaaa gcaatgtaac tgaagaaaag gtacttagtt ttattaagct
     6061 atatgttgga attggagagg taaattatag tttagatgag gtgcctttta cacctagatg
     6121 caaaaagatt ttagatagaa gtgttgaaat tgctaaggaa tatggtcata gcatttcaag
     6181 accagagcac atattattag ctttaataaa agaaaaagaa ggtgtagcta atttaattct
     6241 aactaaaata gtaaaaaatg atataaaaaa tataagtgat aagttaaata acatactagg
     6301 tagaattcca agtaaggaaa gtaaaagcct taaagaaaaa gaaaaaccaa aaagaaaaga
     6361 tactcctaca ttaaatcttt atggtataaa tttaatagaa aaggcagata aaaataaact
     6421 tgatcctgta ataggaagaa aagatgaaac tcaaaggctt ttagaaatac tttgtagaag
     6481 aatgaaaaac aacccttgtt taataggaga acctggagta ggaaaaactg ccgttgtgga
     6541 gggcttagct caaagaattc tattaggtga agtaccagat attattaagg ataaaagaat
     6601 atttacctta gatgtgactt ctatgttagc tggggcaaaa tatagaggag aatttgagga
     6661 aagacttaaa aaggttttaa aagaggtaaa agattcagat gatataatac tctttataga
     6721 tgaaatgcat aatattgtta gggctggggg agcagaaggt gctatagatg catcaaatat
     6781 attaaaacca gctttagcaa gaggtgaaat acaatgtata ggtgcgacaa caactgatga
     6841 atatagaaaa aatatagaaa aggattctgc tttagagaga agattccaac cagtagttgt
     6901 aaaagagcct agtaaagaag attccgtatt aatattaaag ggattgagag aaaagtacga
     6961 agtacatcac aatgtaaaat taacagatga agccatagag gcagctgtta atttatcaag
     7021 tagatatata acagatagat ttctaccaga taaggccata gatctaatag atgaggctgc
     7081 ggcaaaggta agaatagaaa gtatgattgt tcctcctaga ctttttaagc aggaagaatt
     7141 aatagctaac ttaaaaattg aaaaagaaga agctataaaa ttacaggact ttgaattagc
     7201 tgcaaagtta agagatgaag aatcaatgtt acaggaagaa ctagagcgag ataaagaact
     7261 ttggagagaa gaaaattata ataagattta tgaagttaat aaagaacata ttgcaaaggt
     7321 tgtttcaaag tggactcaga taccagtaga aaggcttaca gagaaagaat caagtaaact
     7381 tcttaagcta gaagaaaagc ttagtaaaag agttatagga caattagaag caataaaaac
     7441 catatcaaag gctgtaagaa gatcaagggt aggtcttaaa gatcctaaga gacctattgg
     7501 atcctttgcc tttttaggac ctactggagt aggaaaaaca gagctttgta aggctttagc
     7561 agaggctatg tttggtgatg agaataaatt tattaggctt gatatgtctg aatatatgga
     7621 aaagcatggt gtatctagac ttattggagc ccctccaggg tatgttggat atgaagaggg
     7681 aggacaactt acagaaaagg taagaagaaa tccttattca gtaatacttt ttgatgaaat
     7741 agagaaagcg catccagata tatttaatat tcttcttcaa atattagaag atggtgttct
     7801 tacagatgga aaaggtaaaa ctgtagattt taagaataca ataatcataa tgacttcaaa
     7861 tattggagca gataaattag ataagaaaaa tgcagttgga tttagtgcta aagaggataa
     7921 ggaacgagat tatgataaaa tgaaaggaat tatgttagaa gagcttaaac aaaattttaa
     7981 acctgaattt gtaaatagat tagatgacat agttgtattc catagtctag aaaaggatca
     8041 tcttttaaaa attgttgatc ttatgatatg ttctctaaaa gaaagattaa atgatttgca
     8101 actctctttg aatttttctc aagagtgtaa agaattttta gttaaaaagg gtactgaatt
     8161 aaaatatgga gcacgtccac ttagaagagt tataactaaa tatattgaag atagattaag
     8221 cgaagaactt ttaagtggaa atatattaag aggatctaag gtagatgttt tcttagaaga
     8281 cgaagttgta aaatttaaaa aaactgtttg attttaagaa taaataaggt tatatatgat
     8341 aaaacatata tgaccttatt ttttattatt tatttatata ttgtatttta aatttcagaa
     8401 tataatagag tttttttgtt agaataaaaa ataaaacaaa gaaaaaatat aattaataat
     8461 aattattatt gaaaaattgt ttaacaagga gtataattaa taaaaatagg taccctaagg
     8521 gggtacatcg aataatatat catataatac aaataataaa tttaacctca gtaaggagtg
     8581 ttagtatgga taaagaaata aaagaattag atttaataat aattggagcc gggccagcag
     8641 gattaacatc tgcaatatat gcttcaagag caaaattaag tactttagtt ctagaagata
     8701 atttagttgg gggtcaagta agatcaactt atacagttga aaactatcct gggtttacag
     8761 aaataacagg aaatgacctt gcagatagaa tacaagcgca agcagaggct tgtggagcta
     8821 taatagatga atttgatttt atagaaaatg tctcattaaa agatgatgaa aaaatcatag
     8881 agactggaga ttatatatat aagccaaagg ctgtaataat agctacagga gctacaccta
     8941 agaagttacc aattccaagt gaaagtaaat atctaggtaa gggtgtgcat tactgtgcag
     9001 tatgtgatgg agcagtttac caagatgagg ttgttgccgt tgtaggtggc ggaaatgctg
     9061 ctttagaaga agctttatat ctatctaata tagttaagaa agtaattgtt ataagaagat
     9121 atgattattt ccgtgcagaa gctaagacat tagaggctgc aagcaataag gaaaatattg
     9181 aaataatgta taactgggat ttagttgatg ttctaggtgg agagtttgtt gaagctgcaa
     9241 gaataaaaaa tactaaaact ggagaagaaa aagaaattgc tataaatgga gtctttggat
     9301 atataggaac agaacctaag acatctatgt ttagagaata tataaatgtt aaagaaaatg
     9361 gttatattga aggcgatgaa aacatgagaa ctaatgttaa gggtgtttat gtagcgggag
     9421 atgttagaga aaaaatgttt agacaaataa ctactgccgt ttcagatgga actatagcag
     9481 ccttacatgc agaaaaatat atttcagaaa taaaggagag ataagtatgg taaaagtata
     9541 ttcagttgaa tggtgtggac catgtaataa attaaaaaaa tatttagaaa aattaggcgt
     9601 tcaatacgaa acaataacag ttgcagatgg taaggaagat agaaatgaag ttttagaagt
     9661 ttcaggacaa agatcagtgc cagtagctgt tataggagaa gaagttatag ttggttttga
     9721 taagcagaaa attgatgctg caatagaaaa aataagatag tagaaggtga ttcttaatga
     9781 atactaaaga aaatattttt tataacttta tgggtggtaa tttctatgaa aacaaatcag
     9841 ataaattaat agatataaaa tcacctttag atggtagttt agttggaagg atacaaagtc
     9901 tttctaaaga agatgtggat aaaattattg ataatgcaga aaaagcacaa aaatcatgga
     9961 atgaagttcc tttaaatgaa agagcacatg tgctttataa aactgcagat ttattagaag
    10021 agcataaaga ggaattagca aatataatga taagagaagt tgctaaggac aaaaagagtg
    10081 ctttatcaga agttagcaga acagctgact atataagatt tacagctgat gctgcaaaga
    10141 gtatggatgg agaaagtata ccatcagata acttcccagg atttaacaga agtaaaatat
    10201 ctgtggtaac aagagaacct ttaggggttg tattagcaat atcacctttt aactatccag
    10261 taaatttatc agcatcaaaa atagcacctg ctatagtagc tggaaacagt gttgtgctta
    10321 aacctgctac tcaaggaagt ttatcagctt tattcttagc taaaatgttc catgatgcag
    10381 gtcttccagg aggagtttta aatactgtta ctggaagagg aaatgaaata ggagactatt
    10441 gtgtaactca tgataaaata gacttcataa acttcactgg aagtacagag ataggaataa
    10501 gaatttcaga aataacagtt atgaagcctc ttcttatgga actaggtggt aaggatgctg
    10561 ctatagtttt agaggatgca gatttagatt taactgttaa taatataatt gctggtggat
    10621 attcttactc aggacaaaga tgtactgcag taaagagaat attagtaatg aattcagttg
    10681 cagataaatt agtagagaaa ttaaaagaaa aaataagcaa attaaaaatg ggaaatccat
    10741 taaaagtaga agatgtaaca atagttccgc ttatagataa taaagcagct gactttgtta
    10801 tggaacttat tgatgatgca aaagaaaaag gtgctacttt agtttgtgga ggatatagag
    10861 aaggtaactt aatagaagct actttatttg ataatgttac tacagatatg agattagcat
    10921 gggaagagcc ttttggacca gttttaccta tcataagagt aaatgataag gatgaagcta
    10981 taaaaatagc taatgaatct aaatatggat tacaatcatc agtatttact agagatataa
    11041 atgaagcatt ttatattgct aataaattag aagttggtac tgttcatgtt aacaataaga
    11101 ctgaaagagg accagatcac ttcccattct taggagtaaa atcttctggt atgggaactc
    11161 aaggtataag atattctata gaagcaatgt ctagacctaa ggcaacagta atcacaataa
    11221 gataataatt ttgcaattat tatttacttc aacctatacc taaaaagcta caagtagaaa
    11281 tttacttgtg gcttttttac tgcaaaaaaa tagagagatg taaaaacatc tctctatttc
    11341 tatgttccta ttcacttaaa gctttatcta tagcattttt atcgaaacca acaataatag
    11401 ttccatttat atctaaaaca ggaacaccca tttgtttact ctttttaagc atatcttctc
    11461 tagcaaccat atctgtttgt acgtttaaat cttcaaattc aacatttttt gatttaagat
    11521 attgttttgc tttaacgcac catggacaag agtttgttga ataaaccttt accatattat
    11581 catctcctat gcgaattaat ttttaatata tttttatttt aagtattaaa agtgtataaa
    11641 atatgtgatt taaatcaata aaaaataatt atttaatttg atcatcattt aaatggtcat
    11701 taaatattaa attatgcatt gcatcttcat tgttgttaat aaaagaagat aaactagtta
    11761 gggcagtatt agaagaatgc aatgggtttt cataaacctt atagttatta ctaaatttat
    11821 catttaagtt caatggaatc cctcctaatt tttatttata gtatttgata aaatttcaat
    11881 agatattcat tagtatttat aaaagtggaa aatatttaat gataaatgat aaaatataac
    11941 atagactaaa tatgaaatta ttacaaaaag aatgtatagt ggtctagaca agttagaacc
    12001 agttgagtat aatttaatta ggaggagaaa tcatgataga taaaaatagt cctatacctg
    12061 tgtattacca attaaaagag atgataaaag aaaagatagt agatggtact tggcaagtag
    12121 gacagtgtat agcaagtgaa agagagttaa cagaggctta tggagtaagt cgaatgactg
    12181 tcagacaggc tttaggagaa ctagtacaag aaggtcttct tgttagagaa aaaggaaaag
    12241 gaaccttcgt atgtgaacct aaagtaaagc aagaagatat gatgagtttc tcagaaatta
    12301 ttaaaaaagc aggaagaagt ttaataacta aagttataga ttttgaagtt attaataccc
    12361 ctgaagaatt agcagatact tttgacttag aaaaaatata taaaataaat agattaagat
    12421 tagttgatgg agaagctgtt gctaatgagg ttgtttatat tccttgtgag tattgtggtt
    12481 ttgtaactag agatgattta gaaggatcct tatttaagtt attagaagaa aagggacata
    12541 ttatagaaag tacagaatca tctttagcag ctgtaataat ggacgatgag ctaaaagctt
    12601 tatttaatgt taataaacaa gtacctcttt taaaaagtca taacacagct ttaaatacag
    12661 aaggcgaaat aatttatgtt gaagatgcaa tttataggtc agacaaatat atattagaaa
    12721 ttaatatttc taaaagagag gggaaattga aatgagatta atcgttacta agaattatga
    12781 ggaaatgagt aaagttgcag ctaaagaaat ggctgaagat ataaaaagaa atcctgaaat
    12841 tgttttagga ttagctactg ggggaactcc agtcggaatg tataaagagc ttattagaat
    12901 gtacaatgaa ggagaattag atttctcaaa agttacaagt ataaatttag atgaatatgt
    12961 tgggttatca ggagatcatg atcaaagtta tagatacttt atgaatacaa acttatttga
    13021 ccatataaac atagataaaa ataatacatt cgtacctaat ggattagcag aaaatattga
    13081 agaagagtgc atggcatatg atgctagaat tcaagacatt ggaggaatcg accttcagtt
    13141 actaggatta ggtgcaaatg gacatatagg atttaatgag ccaggagaag ctttaagtgt
    13201 aggaactcac ttaactgatc ttaaagaatc aactatagaa gcaaatgcaa gattctttga
    13261 ttcaattgat gatatgccaa gaaaagctat aactatggga cttggtggaa taatgaaggc
    13321 taaaaaaata atggttatag caagtggaga aggaaaagct gaggttgtaa aggcaatgat
    13381 gagtggaaaa ataacaactg aaattccagc tactatgtta caaatgcata gagatgtcat
    13441 attaatagtt gatgaagatg cagctaaatt attaaaatag ttattactat cttaaattaa
    13501 tatataagaa gactgtttgt atataaaagc attatttaag tgcttaaatt tgcaaacagt
    13561 tttttatttt aataattatt attaaatctt ttacatgaat ctttttaaga aaatagtaat
    13621 aaataagtat gtgattagtt ataatgatat gatatgatta tttaaggata tataaggaaa
    13681 taattagatt tgtacgtaaa atgaagaaat atggagttgg taaagtggca aaggttagaa
    13741 gcatatatgt atgtcaaaat tgtggatatg aaacacctaa atggatgggt aaatgtccag
    13801 aatgtaataa ttggaatact ttagttgaag agattagaga tgctaagagt aatcaatctt
    13861 ctcctaaggt tgaaagacaa attggtgagc ttaaaaagat aaaagaaatt aaatcaggtg
    13921 aaaaggaaag atatgatact ggaattggtg aattaaatag agtattaggt ggaggtcttg
    13981 ttaagggatc gcttacactt atatctggtg accctggaat aggtaaatct acacttcttt
    14041 tacaaacagc caataatatt tcaaaaaaat atgggaaagt tttatatgtt tctggagaag
    14101 aatcagaaga acaaataaaa ataagaggag acagattaaa agttgatgca gaagaacttt
    14161 atatagtttc cgaaactaat cttgatgtaa tagaagcata tatagataaa cttgaaccag
    14221 ctttcataat aatagattca atacaaacaa tttatagaga aacagtttct tcagcaccag
    14281 gaagtgtttc acaggttaaa gaatgctcaa atgctgtaat gagaattgct aaagggaaaa
    14341 atattccttt atttatagtt gctcatgtta ctaagcaagg agatttagct ggaccaagag
    14401 tattagaaca catggtggat actgtattat cctttgaagg ggaaagaact gaagagttta
    14461 gaatattaag aaccatgaaa aatagatttg gtactacggc tgaaataggt gtttttgaaa
    14521 tgcgtggaga gggattaatt caggtttatg atccatctag catgttctta gaggacacta
    14581 gttttaatca agaagggtca gttgtaattg gagtcatgga aggaactaga cctattcttg
    14641 tggaaataca atcattagca tctgaaacaa aagcagttat gcctagaaga acctctgtag
    14701 gagtagaaaa ttcaaggtta agtttaatat tagctgtatt agaaaagaaa ttaagagttc
    14761 ctttttataa tactgatgtt tatgtaaatg tagtaggagg acttgaaata gaaggtacta
    14821 cagcagactt aggaattgcc atttcacttg tatcctctgt taaaggtaag gcagctagat
    14881 tagaaaaatt agttgttgta ggcgaggttg ggttaacagg tgaaataaga ccaatatcaa
    14941 attgtgacag aatattaaat gaagcagaaa aaatgggatt ttcaaatgca gttgttcctt
    15001 atagaagctt agaaaaacta aagggaagta aattaaatct tataggagta aaaactgtaa
    15061 gggaagcaat aggaaagata ttttaagacg gataggtgtg agtaaatgag aactgtacat
    15121 gatgatgagt taaaaaaaat attaaaaata atgagtccag gtacttcttt aagagaagga
    15181 ttagataata tacttagagc taaaacagga ggacttatag tattagggga taatgaagaa
    15241 atattagatt tagtagatgg tggatttaat ataaattctg aatatagtcc agcatatata
    15301 tatgagcttg ctaagatgga tggggcatta gtacttactt cagacagaaa aagaatactc
    15361 tacgcaaatg ctcagcttat gccaaatcaa tctataagta cttttgaaac aggaacaaga
    15421 catagaactg ctcaaagggt tgcaaagcaa acaaataaaa tagcaatagc tatatcacaa
    15481 agaagaaata taattactgt ttataaaggt gacataaaat acgttttaag ggatagtgca
    15541 gttatattaa gtaaagcaaa tcaagctata caaacattag agaagtatgt agctgtactt
    15601 gaaagagtaa caaataatct taacatactt gagtttcaag acctagcaac tctttttgat
    15661 gtaactacag ctatacagag aactgaaatg gtaatgagaa tagttgaaga gatagagggt
    15721 tacattattg agttaggaaa tgagggaaga cttatttcta tgcaattaaa tgaattagta
    15781 agaagtatag aacaagatgg agtactttta ataagagatt attgctatga caagatggaa
    15841 tataatgatg tctacaaaga aattcaagag ttatcggctg aagatttatt agatttagat
    15901 ataatagcaa aagaattggg atatgtagga aaatctttaa ttgacacatt agtgtcacca
    15961 agagggtata gaatatcaag taagatacca agaatacctt caaatgtaat agaaaactta
    16021 gtaggacatt ttggaaaact taaatatatt cttgaagctg gaaatgaaga attagatcaa
    16081 gtagagggaa ttggagaagc tagagctaga gcgataaaaa atggattaag aagaatacga
    16141 gaacaagttg ctttaaataa aaatttataa aaagaagtgc catataaata tggcacttaa
    16201 tatttatcta aaagtaaatt tacctaaagt atccattttg ttatattctt ttagtaaaat
    16261 atcaaatagg tttaaatcaa gtgcattgca tagtgaagtt aaatagaaaa gattatttcc
    16321 aagttcacgt tctataactt ctctgcaatt ttcgcataag tcacctttta tgtgagaatt
    16381 taagcgtgga ttaagttctt ctaaggatag gtcagtatcc tctagcatat tctgtttttt
    16441 agcagaaatc tctatgcaac cgcaatttgt aacggcttta catatagctc tgctagctct
    16501 tgcttgggac tcttgatatt tactcaatat atctaatatg cttttgtgtc taagtaaaga
    16561 attattaacg ctattttgaa aatcgtcaaa aattacgtcc ttcatagtgt ttcactcctt
    16621 atatactaat ctttggccct atagtaatta tagaattttt tggttatatt tgtcaaatga
    16681 tttaggttgc aaaattaaat atatgttaaa aactaattag cttgttaact taactttaat
    16741 ataaatcaaa tttaaaaaga tttatagggg gtaatttatt aattataata attaaacaat
    16801 atttgtaagt attaaagtgc atcaatgtgg tataattaag aaaatatgtt ataaatattt
    16861 tacaactatg aatgataaaa actaaacatg tcaataatta tataggaggt gagtttgtga
    16921 taaagaaaat ttttagagta attttcacta ttttagggtt gttgattgga tttgttttag
    16981 cagacggaat aagaaaaagt gctataggca atgaatattt aacaaatatt tttttaaagg
    17041 tatcatttta tgtaatttta attgctgttt ttggaatcat tttatttatt ctttctcctt
    17101 ttatttataa atctataaga aaacttataa catttataga aaaaggaata tctaaattat
    17161 ctccaagtga aataatattt ggagcggggg gagctatagt agctctagtc ttagctttag
    17221 taataacatt gccattaagt agctttttaa gtggcttaaa tgaaatgtta ggacctattg
    17281 tgtcagttat cataaacata atagctatgg ttataggagc tgatatagct gttaagaaaa
    17341 gagaagatat tacaggatac tttgagaatt ttagaaaaaa tcaatctaag gaaaagaaaa
    17401 caaagaattc ttctaacaag aagaatgaag ctactccaaa ggttttagat acatctgtaa
    17461 taattgatgg tagaatcttt gatttatgcc aaactggttt tatagaggga gctttagtaa
    17521 tcccaacttt tgttcttgat gaattaagac acatttcaga ttgttcagat tctttaaaaa
    17581 gaacaagagg aagaagagga ttagacatat taaataaaat ccaaaaagag cttcctatag
    17641 aagtaattat gtgggaaggt gatttcccag aaatagcaga ggttgatatt aaacttctaa
    17701 aattagcaca aaaattaaat ggtaaagttg taacaaatga ttataactta aataaagtag
    17761 ctgaatttca gggtgttcca gtattaaata taaacgaatt agcaaatgca attaagccag
    17821 tagtattacc aggagaagag atgactctta cagttgtaaa ggatggaaaa gaatctggac
    17881 aaggggttgc ttatcttgat gatggtacta tgattgttgt agaaggtggt agaaagtaca
    17941 tgggaaaaga aataacagta gttgtaactt cagtacttca aactgcagca ggaagaatga
    18001 tatttgctaa atataaatct taagaggaat ttaattatgg gaaaagtagt tagcgtaata
    18061 ttagcaggtg gaaaaggaaa aagaatgggg gctgaagtta gtaagcaatt cattgaaata
    18121 aatggaaaac ctataatata ttatacttta aaagcttttg aggagtgtaa agatattgat
    18181 gaaataatac ttgttctacc taaggatgaa atagattatt ttaaaagaga aatagaacct
    18241 agatttgatt ttaaaatatc taaaattatt gaaggtggaa aggaaagaca ggattcagtt
    18301 tacaatgcat taaattccat aggagattgt gatattgttt taatacatga tggagcaagg
    18361 gcttttgtaa gcaataaaat aatagaagac ggaataaagt attctagaga gtttggagca
    18421 gcagctccag gtgttatgcc aaaggacact ataaaagtta aaaatttaga aggattctct
    18481 gtggatacac caaatagagc atctttagta gctgttcaaa ctccacaatg ctttaaatat
    18541 aatttaataa aaaaaggtca taacaaagtt aaaaatgaaa agattcaagt tacggatgat
    18601 actatgatag tagaactttt aggagaaaaa gtttatcttt ttgaaggtga ctataaaaac
    18661 ataaaggtta caacaccaga agatttaata ttagcagaac attttgttaa gtaaggatca
    18721 caagatagag acattatatt gacatgttat ttttataagg atataatttt ataagattta
    18781 agaaaattta atagttgtaa agactgtgaa aaagattagt agaaacgctt atacagagag
    18841 aaaatactag gctgagagat tttcaacatg ttttgaacct actttggagt cgtagcaaaa
    18901 aactaaccgg gtttaatacc gttaacaatg agtagagtac aaatttaggt ggtaccgcga
    18961 aatacaagct cgccctaaag ttttttaggg tagagctttt tttatttttt aaatgaaatt
    19021 aattaaaata ttaagattat aaatagctta tgaggaggta ttatattatg aaaatgtcaa
    19081 atatgttagt aggaacttta agagaagttc cagctgaagc agaaatagaa agtcacaagc
    19141 ttatgcttag agcaggtctt atgagaaaga tggctgcagg tatttataac tatatgcctt
    19201 taggattaaa ggttatagaa aatgttaaaa atatagtaag agaagaaatg aataatgcag
    19261 gtgctcaaga atttttagca tcagctttaa taccagctga gttatggcaa gaatcaggaa
    19321 gatgggatgc ttatggagca gaaatgttta gattaaaaga tagacataac agagattttt
    19381 gcttaggacc aactcacgaa gaggtattta ctgatatagt tagaaatgaa ataaaatcat
    19441 ataagcaatt accattaaat ctttatcaaa tacaaactaa gtatagagat gaaagaagac
    19501 caagatttgg agttatgaga tcaagagaat tcataatgaa agatggatat agctttgaca
    19561 aagatcaaga aggattagat ttagcatatg aaaaaatgag aaaagcatat gttaatatat
    19621 ttaatagatg tggattagac gctaaggcag ttgcggctga ttcaggagct ataggtggat
    19681 caggttcagc tgagtttatg gttaaatcag aagtgggaga agatgatgta gtattctgta
    19741 cagcttgtga ttatgcagct aacatagaaa aagctccatc aacaccagaa catgcagaaa
    19801 aagaagaatt aatggaagta gaaaaagttg aaactccagc tgtcaaatca attgaagatt
    19861 tagcaaaatt ctttgaatgt tcaccaaaga aaatagcaaa aactttaata ttccaagctg
    19921 atgataaagt tgttgcggtt gtattaagag gagacaggga agctaacgaa gttaagatag
    19981 ctaatgctat tggagaagtt atagaattag aaatggcaag cgaagaggct gttaaagaag
    20041 ctactggcgc agctgttgga tttgcgggtc ctatgggaat aaaagtagat attttattag
    20101 ttgaccaaga agtagctaat atgtataact tcataattgg tgctaatgaa actgatatgc
    20161 acttaaaaaa tgtaaactat ggaagagact ttgaaggaat agttggtgac tttagaaatg
    20221 ttactatagg agaaaaatgc cctgagtgtg gaaaagaaat aactatttca agaggtactg
    20281 aagttggaca tatatttaaa cttggaacta agtattcaga gtctatgggt gcaacattta
    20341 ttgatgaaga tggaaaagct aaaccattta taatgggatg ctatggaata ggggttacaa
    20401 gaactgtagc ttcaataata gagcaacaca atgacgaaaa cggaataata tggccattag
    20461 aagtagctcc ataccatgta tcagttatac cagctaatgt taaaaatgaa gaacaagcaa
    20521 ctaaagctga agaaatatac aatgaattaa gaaagatggg agttgaagct ctacttgatg
    20581 atagaaaaga aagagcagga gttaaattca aagattctga attaatggga attccaatga
    20641 gaataactgt tggaaagatg attggtgaag gtcaagttga atttaaactt agaaatggtg
    20701 gagaagttga gactttatct atagaagaag tttataatag agtaagaaaa gaatttgaaa
    20761 gagaaaattt atctttataa tttataagat taacggaatt gtatataatt agaatataat
    20821 aatagagctc accttattta atatagggtg agttactaat aatataagat aggagggtca
    20881 agtagtgatt aaggtttata atactttaaa caagaaaaaa gaagaattca ttcctttaac
    20941 tccaggagaa gttaaaatgt atgtttgtgg tcctactgtg tacaactttt ttcacatagg
    21001 aaatggtaga acatttatag tttttgatac tataagaaga tattttgaat acagaggatt
    21061 caaagttgat tttgttcaaa actttacaga tatagatgac aagatgataa aaaaggctaa
    21121 tgaagaaggc actactgtaa agaagattgg agatacttat ataaaagagt attaccaaga
    21181 tgcagatgca ttaaatatag aaagagctac agttaatcca agagctacag aatttattgg
    21241 agagataata aaatttgtta agggattagt tgataaagga tatgcttatg aagttgatgg
    21301 agatgtttat ttctcaacta agaagtttga gggatatgga aagttatcag gacaaaacat
    21361 agaagatctt caatcaggtg ctagaataag tgttgatgaa agaaaaaaag atccaatgga
    21421 ctttgctata tggaaagctc aaaaaccagg tgaaccagct tggaatagtc cttggggaat
    21481 gggaagacca ggatggcata tagaatgttc atgcatggct aaaaaattat taggtgaaac
    21541 tatagatatt cacgctggtg gatcagactt aaaattccct caccatgaaa atgaaatagc
    21601 acaaagtgaa gctttaacag gtgaaccttt tgcaagatat tggttacatt cagcctttgt
    21661 taatgtaaac aatgaaaaaa tgtcaaaatc attaaataat ttctttactg caagagaaat
    21721 attagaaaga tatgatgctg atgttataag atttttaatg ttatcagctc actatagaca
    21781 acaacttaac tttagtgaag acttattaga gtcagctaaa gcttcagttg aaagaatata
    21841 caatgctata ggaaacttag aaaacttaat agatgaagtt tctagagaag aaatgaatga
    21901 ggaagaaaaa gcttacttag agtcattaaa taaatataaa gaaaaatata ttgaaaaaat
    21961 ggatgatgat tttaatacag cagatgctat aacggctata tttgatttaa taaaagatac
    22021 taatacaaat ataacaattg actcatcaaa ggaattagct caaaaagctt tagagttaat
    22081 aagagaattg ggagctccat taggaatgtt ccaaaaatca actaaaggaa atctagaaga
    22141 agagattgaa gctttaatag ctaagagaca acaagctaga aaagatagag attttgcttt
    22201 agctgataaa ataagagatg aattaaaaga tagaggaatt gtcttagaag atacacctca
    22261 aggtgttaga tggaagagga tataatttag ataataggct tgcaaaggaa ctttcttttg
    22321 caagttatta ttttaaagaa ttttatttga taactattaa aataatagta aattcaataa
    22381 tttaaaaaaa tctaaattta atcatattaa aatctttgat tgaattagat ttgaatttgt
    22441 agtttaatag ttttaaataa ataaattcta aataattttt aaattaagct caaacaaaag
    22501 gagataagat attgaagagt ttattataca gagagttttc agaaagtgaa gctagaatgt
    22561 taaacccact tcaattagct ttaataggag atggagttta cgaagttttt ataagaaact
    22621 atatattaag tgaaaatgca ggtttatcag ctcacaaaat acatgttaaa gcaatacaat
    22681 atgtaaaagc taaggcgcaa agtgacataa taaattcttt agaaggtgag ctaagggaag
    22741 atgaacttta tatttataag agaggtagaa atgctaagtc agcaacagtg cctaagaatg
    22801 caaatgttag agattataga aatgcaacag gatttgaggc tttagtaggt tatttatatc
    22861 ttactggaaa agaagaaaga ttaaatgagt tcttattgaa atgtatagaa gttcacgttt
    22921 aggaaggaga aatattaatg ttaaaagtta aattattaca atatacacca gaaccagaaa
    22981 agaccatagc agcagcggct aaattatgct atagtcctgt aggtgttgat gatatattag
    23041 aaaatttaac tgatgaaggc gcggaaaagt ttttaaatat gcttatgtca tatggtcata
    23101 tgtcaccaat agagcatgtt tcatttactt ttgcagtaga aggagtttca agaagcttaa
    23161 ctcatcagtt agttagacat agaatagcat cttactcaca gcaatctcaa agatatgtta
    23221 agttagatca atttgaatac atagtaccac cggctataga gaatgatgaa gttgctaaaa
    23281 agctatttat tgatcaaatg caagatgcac aaaaggcata tgatgaaata gttgaaagat
    23341 taaaagataa atatattagt gaaggaattg gtgaaaaacc agctgagaaa aaagctatag
    23401 aggatgcaag atatgttttc cctaatgctt gtgaaacaaa aattgtattt actatgaatg
    23461 ctagaagttt aatgaacttc ttccatcata gatgttgtga tagagctcaa tgggaaataa
    23521 gaactatggc agaaaaaatg gttgatgaag ttaaaaaggt tgccccaata ttatttaaga
    23581 atgcaggtcc aagttgtgta gcaggtccat gtcctgaagg aaaaatgtgt tgtggaaagc
    23641 ttaaagatat gagaacatta tatcttggaa aataggaatt tttaaggaga gaacaaaatg
    23701 acaaagtcag aaaataaata taaacaatta actcgtaagc aaaagtatgc tttagaaaga
    23761 gaacaaagag caagagaaga aaaatatgct agaaatacta gaacttttgc tgaggaagaa
    23821 gttaaggtaa gagaagattt agtagaagga agaaatgctg ttatagaatt attaaagtca
    23881 gataagacta tagagcaaat atttatagct aatggaaaaa tggaaggttc aataaacaaa
    23941 attttaggat tagctaagga aaagaaaata gttgtaaaag aagttgatag aaagaaactt
    24001 gacttaatga gtgaaactaa agctcaccaa ggagtaatag cacaaataac tccttttaaa
    24061 tatagtactg ttgaagatat attagattta gctaaaagta aaaatgaaga tccattcata
    24121 gttattttag atgaattaga ggatcctcat aacttaggat caatagtaag aacagcagaa
    24181 ttatgtggtg cacatggtat cataattcca aagagaagaa atgttggagt aactggaact
    24241 gtttataaag cttctgtagg agctattgaa catatgaaaa ttgcaaaagt tactaatata
    24301 aataatgcta ttgaagaatt aaaggctaac aatgtatgga tatatggagc tgatatagct
    24361 ggagaagaat atagttatga aacaaacttc aaaggtgctt gtggaataat aatcggtagt
    24421 gaaggtagag gaatgtcaaa cttaactaag aagaagtgtg acaaattagt taaaattcca
    24481 atggttggaa aaataaattc tttaaatgcc tcagttgcag gtggtataat gatgtatgag
    24541 gtattaaaat caagaataat aaagtagaaa aagagtgatg agatgagagt tatatttata
    24601 gatggttata atgttataaa tagttggcct aatctaaaaa agtcaaaaaa ttatagctat
    24661 gacatagcaa gaacagagct tacagaaatg cttgaaaatt atgctgcttt taactcttgt
    24721 aaaatttatt tagtttttga tgctcataag gttgctggaa atattgaaag caaagagtta
    24781 tctggaaata taattactgt ctttacaaaa gaaggcgaaa ttgctgatgc atatattgag
    24841 agaatggtag atgaactagg aagaaaagta gaaattgttg ttgtaacctc ggataggtta
    24901 gaacaacaaa ctattttcca aagaggggcc gtgagaatgt catctattga attctacaat
    24961 gaggtgttaa aagttcagga taacattaga aaaaaggcag aaaaaaataa attagaatta
    25021 ggtaaaaatt ttttaggtga tactttagaa gaaagtgtat tagaaaaatt agaaaaaatg
    25081 agaagagggt aatattgact aagtcatata taaaccttat aatttatttg taatttattg
    25141 gatattaggt ttttatctgg gggtgtagag aatatgggga ttacttatga tttggctaaa
    25201 ataaataatt atgaagaatt taaagattta acagataaag aagtagttat gttagctaaa
    25261 aacggtgata atatggctca agaatatgta ataagtaagt atgagcagtt tgtaaaagct
    25321 aaatctaaat catattttct tataggggca gataaagaag atatatatca agagggaatg
    25381 attggattat ataaagcgat aagagatttt aatccagata agtttgcatc atttaaaagt
    25441 tttgcagaac tttgtgttac aaggcagata ataactgcta taaaaactgc aacaaggcaa
    25501 aaacatatcc cattaaacac ttatatttct ttaagcaaac ctatctacga ggaagaatct
    25561 gatagaacgc ttttagatac tgtagtagga ataacaatta cagatcctga atcattgatc
    25621 ataggtaaag aagaggttga aaaaatagaa agtgcaatta atagtgtgct ttctgaacta
    25681 gaattagagg tgttaaattc ttatttagat ggtaaatcat atcaagaaat tgcctgtgat
    25741 ttagatagag aagctaaatc tatagataat gcattacaac gtgtaaaaag aaagcttgaa
    25801 aaatgtttaa acattaaata atgcaatttt ttaaaagtat tgacaaagaa ttttattagt
    25861 agtaaaattt ttatggcata ggtaaattaa atgaatgtaa ggtgctcacg tagctcagtc
    25921 ggcagagcgt cgccttggta aggcggaggt cgtcggttca atcccgatcg tgagctccaa
    25981 tccaaaataa ggaaaacaca acacgctagg tagagtttat ttgaatttta aaggaggaat
    26041 ttgcaatgtc aaaagcaaaa tttgaaagaa gcaaaccaca cgttaacatt ggaacaatcg
    26101 gtcacgtaga ccacggtaaa acaactttaa cagcagctat aacaacagtt ttagcacaag
    26161 ctggtggagc agaagcattc aaatacgatg aaatagataa agctccagaa gaaaaagaaa
    26221 gaggaatcac aatcaacaca gcacacgttg agtacgaaac agctaacaga cactacgctc
    26281 acgttgactg tccaggacat gctgactacg ttaaaaacat gatcactgga gcagctcaaa
    26341 tggatggagc tatattagta tgttcagcag ctgatggtcc aatgcctcaa acaagagagc
    26401 acatcttatt atcatcaaga gttggagttg accacatcgt agtattctta aacaaagcag
    26461 atatggttga cgacgaagaa ttattagaat tagttgaaat ggaagttaga gagttattaa
    26521 gcgaatacaa cttcccagga gacgacattc cagtaatcaa aggatcagct ttagtagcat
    26581 tagaaaaccc aactgacgaa gctgcaacag cttgtatcag agagttaatg gatgctgtag
    26641 atagctacat cccaacacca gaaagagcaa cagataaacc attcttaatg ccagtagagg
    26701 acgtattcac aatcactggt agaggaacag ttgcaacagg aagagttgaa agaggagttc
    26761 tacatgtagg agacgaagta gaagtaatcg gattaactga agaaagaaga aaaacagttg
    26821 taacaggaat cgaaatgttc agaaagttat tagatgaagc acaagctgga gataacatcg
    26881 gagcattatt aagaggtatc caaagaactg acatcgaaag aggtcaagtt ttagctcaag
    26941 ttggaacaat caacccacac aaaaaattcg taggtcaagt atacgtactt aaaaaagaag
    27001 aaggtggaag acacactcca ttcttcgatg gatacagacc acaattctac ttcagaacaa
    27061 cagacgttac aggatcaatc aaattaccag aaggaatgga aatggttatg cctggagacc
    27121 acatcgacat ggaagttgaa ttaatcacag aaatcgctat ggacgaagga ttaagatttg
    27181 ctatcagaga aggtggaaga actgtaggtt caggagttgt tacttcaata atcgagtagt
    27241 tttaagcaaa acaacatata gtttatcata ataaggactg ggttaaataa cctagtcctt
    27301 tacatagcca acgtttgttg acacttgtgt atatttatga taatgtattt tagtaattaa
    27361 ctagcagtgt attggtagat agaactactt ttaatataca acaaaactgg aggtgctgaa
    27421 cgtgagagta aaagtaactt tagcatgtac tgagtgcaag caaagaaact acaacactat
    27481 gaaaaataag aaaaacgatc ctaatagaat agaaatgaaa aaatactgca aattctgtaa
    27541 gactcatact cttcacagag aaactaagta gtgttttgga tagaaaattc taatgcatta
    27601 ttatggtgtt aaggatgtga atttatggct acaaagcaaa atgtaaatga aaaaagcaga
    27661 ggctttggta taacaaagtt ctttagaggg gttaaggctg aaatcaaaag aataacttgg
    27721 ccaccaaaag aagaagctaa aaaggccatt attgcagtaa ttgtatttac tgtaatatct
    27781 attgctttaa taggagtaat ggattttgta tttaagaacc tctttgagtt agtgtttgga
    27841 cttaaataat ataagggagg tttggtgatt tattcaccgg tatgatatga gtgatagagc
    27901 tagatggtac gtagtacaca catattcagg atacgaaaat aaagttaaag caaatcttga
    27961 gaagactata gagaacagaa acttagaatc tttaatctta gacattcaag ttcctatgga
    28021 agagagtgca gaagaaaaag atggtaagat aaaagttact ttaaagaaaa aattcccagg
    28081 atatgttata gtaaacatgg ttatgacaga tgaatcttgg tatgtcgtaa gaaatacaag
    28141 aggagtaact ggatttgtag gtccagggtc aaaacctgta ccacttaccg atgaagaggt
    28201 agagtctatg ggagtaatga aaactgctat ggatatcgac ttggaggtag gagaaactat
    28261 aaacattatt tcaggagctt taagtggttt tgctgctata atccaagaaa taaatgttga
    28321 aaaaggcaaa ataaaagcct tagtagatat gttcggtaga gaaactctag ctgaactaga
    28381 ttttaatcaa gtagaaaaat tagattaaaa atcagactaa cgtctcgatc aagtgggagg
    28441 gcggaagtcc gataattacc acagtatagg aggaataaac tcatggctaa gaaagtaaca
    28501 ggaatgataa aacttcaact tcctgcagga aaagcaacac cagcaccacc agtaggacca
    28561 gctttaggtc aacacggtgt aaacataatg ggattctgta aagaattcaa cgcaaagact
    28621 gctgataaag ctggcttaat aataccagtt gtaataactg tatatcaaga cagatcattc
    28681 agcttcatat taaaaactcc accagctgca gttcttataa agaaagaatt aggcttagaa
    28741 agtggatcag gtgttccaaa cagaactaaa gttggaaaca tcactaaaga gcaaataaga
    28801 aaaattgctg aattaaaaat gccagactta aatgctgcaa caatcgaaac tgcaatgagc
    28861 atgatcgaag gtactgctag aagtatgggt gtagtagtag tagaataatt tattcaaatc
    28921 ggtgggaggt aaaaaccgct aataccacaa aggaggatta tattaatggg aaagaaatat
    28981 attgaaagtt caaaattaat agataaaaat gcattatata ctccagcaga agctttagag
    29041 cttgctgtta aaactgcaaa agctaaattc gatgaaacta tcgaattaca cgtaagatta
    29101 ggtgtagacc caagacatgc tgaccaacaa gttagaggag ctgttgttct tccacacgga
    29161 actggtaaag atgttaaagt tttagttttc gctaaaggtg agaaagctaa agaagctgaa
    29221 gctgcaggag cagatttcgt tggagctgac gaattagtac aaaaaattca aggtgaaaac
    29281 tggtttgatt atgatgtagt tgttgctact ccagatatga tgggtgtagt tggtagatta
    29341 ggtagagtat taggaccaaa aggtttaatg ccaaacccaa aatcaggaac agttacattt
    29401 gacgtagcta acgctataaa agaaatcaaa gcaggtaaag ttgaatatag agtagataag
    29461 actgctatag ttcactgccc aataggaaag aaatctttcg gaactgagaa gttagttgaa
    29521 aacttcaaag ctttaatgga cgctttagta aaagctaaac cagctgctgc taaaggacaa
    29581 tacttaaaat cagtttcagt ttcagctact atgggacctg gagctaaagt taacccagct
    29641 aaagttttag attaatttta ataaagtgct tgacataagt ttaagtgcat gttaaaataa
    29701 tttttgttga aaaagtgaat gaaagtttcc gtagacagta ggtgctgaaa ggcataacgt
    29761 agaacaacct accgagggca attatagtgt atataatagt cttcctatgt ctacgggaag
    29821 acttttctta ttatataaag cagttaacta aactgtgagg aggtggacac agtgaacaaa
    29881 aacagacaat taaaagaagc taaagttgca gagataaaag agaaattaga aaaagctcaa
    29941 tcagttgtac ttgctagcta ccaaggttta acagtagaac aagatacaaa cttaagaaag
    30001 agcttaagag aagcaggtgt tgagtacaaa gtttacaaaa acacactagt tactctagct
    30061 gcaaaagaat taggttttga tggaatagtt gaatacttag aaggtccagt ttcaatagct
    30121 ttcggttacg aagatgctac tgctccagct agaatcctta acgatttcgc taaggaaaac
    30181 aaaaagcttg agttaaaagc tgctatagta gaaggaacag tttacaacaa agagcaaact
    30241 gagcaacttg ctgctatacc ttcaagagaa gtgttacttg caaaacttct tggaagtatc
    30301 aagtcaccac tatcaaactt tgcatactta ttaagtgcaa tagcagacaa aaaagaagaa
    30361 tctgctgaat aattaataaa aataaaaatt taaaaaacag gaaagaaaat ttcggaggtg
    30421 cgaatcatga caaaagagca aatcatagaa gctataaaag aaatgagtgt tttagaatta
    30481 aacgagttag ttaaagcttg tgaagaagaa ttcggagtaa gcgctgctgc tccagttgct
    30541 gttgtaggcg gagctgctgc aggtgctgct gcagaagaaa aatcagagtt tgacgtagta
    30601 ttaactaatg ctggtgcaaa caaaataaaa gtaatcaaag cagttagaga attaactggt
    30661 ttaggattaa aagaagctaa agaaatagtt gatggagctc ctaaaacatt aaaagaagct
    30721 gtagctaaag aagaagctga agacatgaaa gctaaattag ctgaagttgg agctgaagta
    30781 gagcttaagt aattaaagct taattttaaa atattaaaag aggtacttag tagtgcctct
    30841 tttttttaac tcaagagatt gggcgtaaaa aataatttaa agtaaatttt tttagaatat
    30901 tgacaacggg tattgcctat gataccataa ataaatgtac tattgtgtaa tagtataaag
    30961 ttgaatatga attttatgta tactacatta aaaaatgggt atactaaaaa gatgatattg
    31021 tatgtaatcc gaaagcatag gtccttaggg aaagtatgct tttggctatt ttagtttata
    31081 caaggggtga aagttgatgg tacatcctgt ccaagttgga aaaagaacga gaatgagctt
    31141 tgcaaaagtc aaagacgtgg ctgaaatgcc aaacctaatt gaaattcaat tagattctta
    31201 caaatggttt ttagatgcag gactttatga agtttttgat gatattaatc ctatctcaaa
    31261 ctttactgga aatcttgtac ttgagtttgt agggtacact ctagatatgg ataacataaa
    31321 atattcagtt gaagagtgta aagaaagaga cactacttat gctgcaccgc taaaagtagc
    31381 tgtaagactt caaaacaaag aaactggaga aataaaagaa caagaggtat ttatgggaga
    31441 tttcccatta atgacagagc aaggaacttt tataatcaat ggtgccgaaa gagttattgt
    31501 ttctcagtta gtaagatcac caggtgttta ctacaattat aatgtagata aaactggtaa
    31561 aaaactgttc tctgcaacag taatacctaa cagaggtgcg tggttagagt atgaaacaga
    31621 ttcaaatgat gttatctatg taagaataga taaaacaaga aaacttccta taagtatact
    31681 aggaagagct atgggctttg gaagcgatca agaacttctt gagtattttg gtgaagaaga
    31741 aagattcaaa gctacaatag aaaaagataa tactaagact aaagaagaag cattattaga
    31801 aatctataag agattaagac caggtgaacc acctacagtt gatagtgcaa ttagtttaat
    31861 agattcttta ttcttcgatg ctaagagata tgacttatca agagttggta gatacaagtt
    31921 taacaaaaaa cttgcaattg gtttaagaat agctaaccaa atagcagcag aagatatagt
    31981 agataaatta actggtgaag ttttagttgc aaaaggtgaa aaaatatcaa gagctaatgc
    32041 tgaagaaatt caaaatagag gaataaattc agttgatgtt ttagttgaag acagagtaat
    32101 tagaattatt ggtaaccact ttgtagatat acataagtgt gtggacttcg atatatcaga
    32161 tttaaatatc agagaattag ttcactaccc aacattaaga gagatcttag acaactattc
    32221 tgatgaagaa actataaaag aagaaatcaa aaagaatatg acaagactta ttcctaagca
    32281 catcatcaaa gatgatatat tcgctacaat aagttaccaa attggcttag cttataatat
    32341 tggatatgtt gatgatatag accacttagg aaacagaaga ttaagaagtg ttggtgagct
    32401 tttacaaaat caatttagaa ttggtttatc aagaatggaa agagtagtta aagagagaat
    32461 gactattcaa gaccaagaag ctataacacc acaacaatta atcaatataa gaccagtagc
    32521 agcagctata aaagagttct ttggaagctc acagttatca cagttcatgg atcaaacaaa
    32581 cccattatct gagttaactc ataagagaag attatcagct cttggaccag gaggtctttc
    32641 aagagaaaga gctggtttcg aagttagaga cgttcaccac tctcattatg gtagaatgtg
    32701 tcctatcgaa actccagagg gaccaaacat aggtcttata aactcattag ctacatatgc
    32761 taaagttaat gagtatggct ttattgaaac tccttataga gttgtagata aatcagaagg
    32821 tagagtaact ggtgaaataa gatactttac tgcagatgag gaagaccaat acctagtagc
    32881 tcaggctaat gaaccattag acgaaaatgg atgctttatt gataagaaag taactgttag
    32941 agataaaggt gaggtattag tagttccaag taaggatgtt gacttaatgg acgtttctcc
    33001 aagacagcta gtatctgttg caacagcaat gatacctttc ttagagaatg acgacgcatc
    33061 aagagcccta atgggatcaa acatgcaacg tcaagcagtt cctttattaa aaccatatgc
    33121 tccaatagtt ggtactggaa tagaatacaa agcagctgta gattcagggg ttcttcctaa
    33181 agctaaaaat gctggtgaag tagtttatgt aagtgctaac gaagtaagag ttaagagaga
    33241 attagatggt ggtgttgata cttatagact tcttaaattc aagagaagta accaaggtac
    33301 ttgtataaac caaagaccaa tagtggctaa aggtgactgg gtacttaaag gtgaagtatt
    33361 agcagatgga ccttcaactg atttaggaga aatagcttta ggtaaaaaca taagaatggg
    33421 attcattact tgggaaggat ataactacga ggatgctatg cttatctcag aagagttagt
    33481 tagagaagac gtatttacat caatccatat agaagaatat gaatgcgaag ctagagatac
    33541 taaattagga ccagaagaaa taactagaga tattccaaac gtaagtgagg atgctttaaa
    33601 agatatagat gaaagaggta taatcagaat cggtgctgag gtaagatcag gagatattct
    33661 tgttggtaaa gtaacaccaa aaggagaaac tgaacttact gctgaggaaa gactattaag
    33721 agctatcttt ggtgaaaaag ctagagaagt tagagatact tctttaagag ttccacacgg
    33781 ggaagctggt ataatagtag atgtaaaagt atttacaaga gaaaatggtg atgatttatc
    33841 accaggagtt aatgaacttg taagatgtta cattgctcaa aagagaaaaa tatctgttgg
    33901 agataagatg gctggtcgtc acggtaacaa aggggttatc tcaagagtat taccagaaga
    33961 agatatgcca ttcttaccag atggtagacc acttcaaata tgcttaaacc cactaggggt
    34021 tccatctcgt atgaacatcg gtcaggtatt agaggttcac ttaggatggg ctgcaagtgc
    34081 attaggatgg cacattgcta caccagtatt tgatggtgct acagaaactg atatagaaga
    34141 ttgcttagaa aaagcaggat acaatagaaa cggaaaaaca gttttaagag atggtagaac
    34201 tggtgaagag tttgataacg aagtaacagt tggtataatg tacatcttaa aacttgctca
    34261 cttagttgat gataagatcc atgctagatc aacaggacct tactcattag taactcaaca
    34321 gccacttggt ggtaaggcac aattcggagg acaaagattc ggagagatgg aggtttgggc
    34381 attagaagca tacggtgctg ctcatactct tcaagaaata cttactgtta agtcagatga
    34441 tgttgtaggt agagttaaaa cttacgaagc tattgttaaa ggtgaaaaca ttcctgaacc
    34501 aggagtgcca gaatcattta aggttcttat taaagaactt caagctttat gcttagacgt
    34561 taaagtttta aatgataata accaagaagt taaattcaaa gaattagctg aagatgatga
    34621 tgaaatcgaa gtattagaag ttaacatgga aggtactgaa gattcaacaa cagaagaagc
    34681 taaagaggaa aaaggggaag catacatccc agcagaagaa attgatgaag aaatagacta
    34741 tgaaaacata gacttattag acttcacaag tgatttagac atagaagatg attttaacta
    34801 ggatataaag tgttcccttg ctcatgagag taaggggata cttaaaaatt cacattaaac
    34861 tatgaaggga ggacacaccc ttgttcgaat taaataattt tgatgcttta caaatcgggt
    34921 tagcatctcc agaacaaatt agagagtggt ctagaggaga agtaaagaaa cctgaaacta
    34981 ttaactatag aacgttaaaa cctgagagag acggattatt ctgtgaaaga atattcggac
    35041 ctataaaaga ctgggaatgt cactgtggaa aatacaaaag agttagatat aaaggtattg
    35101 tatgtgacag atgtggtgtt gaagtaacta aatctaaagt tagaagagag agaatggctc
    35161 acattgaatt agctgcgcca gtatctcata tatggtattt caaaggaata ccttcaagaa
    35221 tgggattaat ccttgatatg tcaccaagag ctttagaaaa agttctatat tttgcatcat
    35281 atatagttat agatccaaaa gaaacttcat tattaaagaa acaacttctt aatgagaaag
    35341 aatatagaga agcgtgtgac aaatatggtg aagagagctt tgtagctgga atgggtgcag
    35401 aagctataaa aactttatta tcagaaatag acttagaaag aactgctgct gaacttaaag
    35461 aagaattaaa gcaaagcaca ggtcaaaaga aagttagaat aataagaaga ttagaagttg
    35521 tagaatcatt caaatcatca ggaaataaac ctgaatggat ggtagtagat gtaataccag
    35581 ttataccacc tgatttaaga cctatggtcc aattagatgg aggtagattt gctacttcag
    35641 atttaaatga tttatacaga agagttataa atagaaataa cagacttaag aagttattag
    35701 accttggcgc tcctgacata atagttagaa acgaaaagag aatgcttcaa gaggctgtag
    35761 atgctttaat agataacggt agaagaggaa gacctgtaac tggtcctgga aacagacctc
    35821 ttaaatcatt atcagatatg cttaaaggta agcaaggaag attcagacaa aaccttcttg
    35881 gtaagagggt tgactattca ggaagatcag ttatcgttgt tggtcctgaa ttaaaaatgt
    35941 atcaatgtgg tcttccaaaa gaaatggctt tagaattatt caagcctttc gttatgaaga
    36001 aattagttga agacggagtt gctcataaca taaagagtgc taagagaatg gttgaaagag
    36061 taatgccaca agtatgggat gtacttgaag aagttatagc agaccatcca gtattactaa
    36121 acagagcacc tactctacat agactaggta ttcaagcatt ccaaccagta ttagttgaag
    36181 gtagagctat aaaacttcat ccacttgtat gtacagcata taacgctgac ttcgatggag
    36241 accaaatggc tgttcacgta cctttatcag ttgaagctca agcagaagct agatttttaa
    36301 tgcttgcagc aggtaatata atgaaacctt ctgatggtag accagtttgt gtacctactc
    36361 aggatatggt tcttggttca tattacttaa caatggataa agacggagct aaaggtgaag
    36421 gaaaatactt cgcatcattt gatgaagtaa taatggctta tcaattaaaa gaagttgata
    36481 ttcatgctaa gattaacgtt aaagttacta aagaaataga cggagagctt aagtcaggaa
    36541 taatcaaaac aactccagga tttataatat tcaatgaatg tatacctcaa gacttaggtt
    36601 ttgtaaatag agaaaatcct gaagaaatgt tcaatttaga aatagatttc ttaataacta
    36661 aaaagtcatt aggaaaaata atagataaat gttacttaaa gcatggtcca actaagactt
    36721 caataatgct tgataacata aaagcaacag gatatcatta ttcatcaatt ggagctgtta
    36781 cagttgctgc ttctgatatg atagttcctc aaaagaaata tgagctatta aaagaagctg
    36841 atgaaactgt agataagatt gaaaaaatgt atagaagagg tcttatatct gaagatgaaa
    36901 gatatgaaag agttatagaa aaatggactg agactactga ggaagttgcg gatacattaa
    36961 tgaatagttt ggataaattt aacccaatat tcatgatggc agactcagga gccagaggat
    37021 ctaagtcaca gatcaaacag ttagctggta tgagaggact tatggcgagt ccatcaggta
    37081 aaatcatcga gttaccaatc agagcatcat ttagagaagg tcttgacgta ttagagtact
    37141 tcatatctac tcacggagct agaaaaggta acgccgatac agcacttaaa actgctgact
    37201 caggttactt aacaagaaga cttgttgacg taagccaaga tgttatagta agagaacacg
    37261 actgtggaac tcaaaatggt atttacgttg aagaaataaa agaaggttca gaagtagttg
    37321 aacaattagc tgaaagatta acaggaagat atactgcaga agatgttttc catccagaaa
    37381 ctggagagct tttagctgca aaagatactt acatggatcc aattttagct gaaaaaatag
    37441 ctgacactgg aattcaaaaa gtaaaaataa ggtcagtatt tacatgtgat tctaaggttg
    37501 gagtatgtac taagtgttat ggtatgaaca tggcaacatc atataaaatt aatattggtg
    37561 aagctgttgg tatcgtagct gctcagtcaa taggagaacc aggaacacag cttacaatga
    37621 gaacattcca tacaggtgga gttgctggag cagatataac tcaaggtctt ccaagggttg
    37681 aagagttatt cgaagcaaga aaaccaaaag gattagcaat tgtaagtgaa gttgcaggta
    37741 ctgtaagaat agaagagact aagaagaaga gaacagttta tgttgttact gattcaggag
    37801 aagaatatag ctatgacata ccatttggat caagattaaa agttaaagat ggtatagcta
    37861 ttggagctgg agatgaaata acagaaggtt cagttaaccc acatgatatc atgagcatta
    37921 aaggtgttga tggtgcaaga gaatacttac tttcagaagt tcaaaaagtt tacagacttc
    37981 aaggggttga tatcaacgat aagcacttag aagttgtagt tagacaaatg actagaaaga
    38041 taaaagtaac agagcaaggt gatactaatt tattaccagg tgtaatgatt gatatgttcg
    38101 atttcagagc tgaaaatgaa agagttgaaa gctttggagg agaaaaagct caaggagata
    38161 tagttcttct tggtattact aaagcagctc ttgctacaga tagtttctta tcagcagcat
    38221 cattccaaga aacaacaaga gttcttactg atgcagcaat taaaggaaaa attgatcctc
    38281 ttgttggatt aaaagaaaat gttataattg gtaagttaat tccagcaggt acaggtatga
    38341 tgaagtatag atcattaaaa ttaaatactg aaaacagtaa tcaagagact gaaacaataa
    38401 ttgagattga agaataagat taattttgtt gacagtagta ttcttagatg ataaaataca
    38461 gtttgtgtga ttagcaaagt actaagggat taacttgtct acatgattat ttacaatgga
    38521 ggtaatctta tggtagacag acttttagga aaaaaagtta ttggcataaa acagtccaca
    38581 aaagctatga aaaacggtga aggaaaaatt ttgtatgttg caaaaaatgc taacgataaa
    38641 atggttactc cattggtaga attagcagaa aaatgtggaa ttgaaataaa acatgttaaa
    38701 aacatgaaaa ttctagggga aatgtgtggc atagatgtaa aatcagctgt agcattaatc
    38761 ttagattaga gtgttaacat ctaaaattta tatagatagt taaataattt atcaggaggt
    38821 gaaagtatgc caactataag ccaattagta agaaaaggca gaaaaacagt agcatcaaaa
    38881 tcaactgcac cagctcttaa agagtgtcca caaaaaagag gagtttgtac tgtagttaaa
    38941 acaactactc ctaagaaacc taactcagcg ttaagaaaaa tcgccagagt aagattaact
    39001 aatggatacg aagtaacagc ttacattcct ggtgtaggcc acaacttaca agagcatagt
    39061 gttgttctta taagaggtgg aagagttaaa gaccttcctg gtgttagata ccacatagta
    39121 agaggagctt tagactcagc tggagtagct aacagaatgc aaggaagatc aaagtacggt
    39181 gctaagaaac caaagcaaaa gaaataattg ttgcttaaac ttagtttaag tgctatgtct
    39241 tcaagtttag ataaataact gttcgaagtt taaatataga ttatgaagca agcactttac
    39301 gacaatcgat taagttgtgt cgagtaccga tgaacttaaa caaaaattgt taaggaggga
    39361 agaaaagtgc caagaaaagg acatattgca aaaagagatg tattaccaga tccagtgtac
    39421 aattcaaaag ttgttactaa gttaataaat aacgtaatgg aagatggtaa aaaaggagta
    39481 gcacaaaaaa tatgctacga tgctttccaa ataatcaatg agaaaactgg cagagatgct
    39541 atggaagttt ttgaggaagc aatgaacaac ataatgcctt tattagaggt taaagctaga
    39601 agaataggtg gtgctaacta ccaagttcca atagaagtaa gacctgaaag aagacagact
    39661 ttaggattaa gatggcttct tgcagcttca agaaaaagag gcgagaagta catgagagaa
    39721 agattagctg gagaattaat ggatgcagct aacaatactg gagcagctgt taagaagaga
    39781 gaagatactc acaaaatggc tgaagctaat aaagcatttg ctcattacag atattaataa
    39841 aatgaaactg ttttggctaa aagccaaaac agttttgttg aatttaaaaa cactatacgt
    39901 tgagaggagg agtcacaatg gctagacaat atccgttaga aaaattccgt aacttcggaa
    39961 taatggcaca tatagatgct ggtaaaacaa ctactactga gcgtattctt ttctatacag
    40021 gaagaaacca caaaataggg gaaactcatg atggagcttc aactatggac tggatggctc
    40081 aagagcaaga aagaggtata acaataactt ctgctgctac aacttgtttc tggaaaggtt
    40141 atgaattaaa cataatcgat actccaggac acgtagactt cacagttgag gttgaaagat
    40201 cattaagagt tcttgatgga gctgttactg ttcttgatgc taagagtgga gttgaaccac
    40261 aaactgaaac tgtttggaga caggcagaca agtacggcgt tccaagaatg atatacgtaa
    40321 acaaaatgga tgctacagga gcagactact acaactgtat aaacacagtt agagaaagat
    40381 tacaagctaa tgctgttgca atccaaattc caataggtca agaagatcaa ttccaaggaa
    40441 tggttgattt attaactaac caagcaataa tcttcaaaga tgacttagga aaagacatag
    40501 aagtaggtga agttccagct gatttagctg acaaagcaga agagtacaga gctgctatga
    40561 tcgaagctat agctgaaact gatgaagagt taatgatgaa atacttagaa ggtgaagaat
    40621 taactcttga agaattaaaa gttgctttaa gaaaagctac tataaacaat gaaataatcc
    40681 cagttatctg tggatcatca tacaaaaaca aaggtgttca acaaatgatc gatggtgttg
    40741 ttgattactt accatcacca ttagatatac ctgctgttaa aggtactaac ttagacggtg
    40801 aagaagaagt tagagaagct tcagatgacg ctccaatgtc agctttagca ttcaaaatag
    40861 ctactgaccc attcgttgga agattagcat tcacaagagt ttactcagga gttttagaga
    40921 gcggttcata cgtattaaac tcaactaagg gcaaaaaaga aagaatcgga agacttgtta
    40981 agatgcacgc taactcaaga gaggaagttg aatcattaga agcagctgaa ttaggagctg
    41041 taataggtct taagaacaca actactggag atactttatg tacagaagca gctccaataa
    41101 tacttgaaaa gatggaattc ccagagccag ttatatctat agctatcgag ccaaagacaa
    41161 aagctggtca agaaaaaatg ggtatagctt tatcaaagct tgctgaagag gatccaactt
    41221 tcaaaacttg gactgaccaa gaaacaggtc aaactatcat agctggtatg ggtgagcttc
    41281 acttagatat catcgttgat agattacaaa gagagttcaa agttgagtgt aacgtaggtg
    41341 ctcctcaagt tgcttacaaa gaaactatca aaaaggctgt tgaagcagaa gctaaatttg
    41401 ctagacaatc tggtggtaga ggacaatacg gtcactgtaa gatagaaatg ataccaactg
    41461 aaggcgaata tgaattcgaa aatgctatcg ttggaggagc tattccaaga gaatacattc
    41521 cagcagtaga taacggaatc agagaagctg cagaaagtgg tataatagct ggatacccag
    41581 ttataaactt caaaataaga ttattcgatg gatcatacca tgatgtcgat tcatctgaaa
    41641 tggcattcaa aatagctgga tctatggcat tcaaaaacgc tatggctaaa gctgatcctg
    41701 tattacttga gcctataatg aaagttgaaa tcactgtacc agaagagtac atgggagacg
    41761 ttataggaga tgttaactca agaagaggta gaatggaagg aatggactca agaaatggtg
    41821 cacaaatcat aagagcattc atcccactat cagaaatgtt tggatacgca actgcattaa
    41881 gatcaagaac tcaaggtaga ggaacttatg caatggaatt cgatcactat gatgacgttc
    41941 ctaagagcat ccaagaagaa gttgcaggta aaaaaaataa ataattaaag aagcttttaa
    42001 agcttcttta atatataaat gtatataata tttttaa
//
LOCUS       DAEVGI010000007        41594 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEVGI010000007 DAEVGI010000000
VERSION     DAEVGI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 41594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41594
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.007"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(65..505)
                     /locus_tag="K8M77_000391"
     CDS             complement(65..505)
                     /locus_tag="K8M77_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBI7084919.1"
                     /translation="MYNDEFIKAEEKRLYDEYKKQIANLKKVQKEKDAVGQVFTKGLL
                     PIYALYILSIQPTNGNDLAQKIGEQTNGLWFPSTGGIYPLLKKLEKQGFVTGSWDDPK
                     RKIQKVYSLTPEGFKEFEEKKHLLKPKIKEALEVFKIIYSHLYN"
     gene            complement(508..1353)
                     /locus_tag="K8M77_000392"
     CDS             complement(508..1353)
                     /locus_tag="K8M77_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBI7084920.1"
                     /translation="MQKIAIITDSSCDLTIDEIKDYNLNILPLKIIYKDREYNDIFDI
                     KPVDVYENLHNEVPTTSLCSPDYINSVLDKLEAEGYTHLIGIFISNSLSGTFNAARLV
                     IEERSSFKYYLFDSKIIGYPLGSMVIKAAEFVKEGKSFEEIIEALPSIREATTGFYTL
                     NTLEYLRRGGRIGKVAGTVGDLLHLKPIISVDEHGAYTTIAKARGRKQSLKKLANIIL
                     EHLDENKCYVAILNGMAEEEANQVLNLISSHPNLLKSQVRAIGAAMGVHAGPGMVGVS
                     IQKEI"
     gene            complement(1613..2680)
                     /locus_tag="K8M77_000393"
     CDS             complement(1613..2680)
                     /locus_tag="K8M77_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HBI7084921.1"
                     /translation="MKKIALIAFQLILSLLVGFIFGNLIMPLKTSEKIDTLKELKPID
                     LTMNEENPYDFDMEELSLETLSPYTGNLLESDSIDNIPFMCIIENSRAARPQSGLSEA
                     DIVYETMAEAGIPRFLAVFYENSPEAIGPVRSVRPYFLDISKEHDLPFAHCGGSAEAL
                     ATISSDKSLNSINEIKNGSAFWRDNTRKAPHNLYTSAKNIRKFIADNGYEYSKNNTLS
                     FKDNSLLNSSNYISEIKLNLSDYYKTSYKYVDGFYEKYMDGELAIDKNTSSSLKFKNI
                     VVQITDMSLQSDNTHLDIDLTGTGEGYVFSNGRMEKIHWEKEDSNSKTTLKTEDGTPI
                     HLSRGNTIWNIVDKNSSLSFN"
     gene            2940..5057
                     /locus_tag="K8M77_000394"
     CDS             2940..5057
                     /locus_tag="K8M77_000394"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBI7084922.1"
                     /translation="MLHVVKRDGRESRFNADKIANAIKRAANEVGIAIRESQVLDTIQ
                     KVINYIEMSGKTDVTVEEIQVLVEKALMDNGFNQVATAYKAYRTERTKVREIKSDLMK
                     AISKIGVETDRDNANVGNNFSSKLLRIASESNKWYNLATMPKELAKAHENGDVYYHDL
                     DSYNLTTNCLHIPTGELLQSGFNTGYGTINAPRRIETAAELSCILLQSTQNDMFGGQS
                     HPDFDNDMSVFVEPTRQEIREEYIGLGLEGAKLDELVEKKLRERVHQAMQGVVYNLNT
                     MHSRAGSQVPFSSLNLGIPYNEDAALVCEIFLLEYEKGLGRGEQPIFPNIIFRVKEGV
                     NREVEDPYFYLYELACRVAAKRMNPTFMNIDADFNKEYYDKGYMPATMGCRTYLMKNV
                     NGEPGCKGRGNIAPTTINLPRLGILAKKDINKFFDLLDDRLRIAKESLMHRYGVLKNL
                     KVKDLPFVAGQGLMKGAENLGPDDSIEPILKQGTWGIGFIGIAETLIALTGKHHGEDE
                     TSRELGIKIVERIREYCDRLTEETRLNWSCYATPAEGLSGKFIKQDKKAFGIIKGVTD
                     KDYYTNSYHVPVYCPMSIKNKIDIEAPYHKLCNGGHISYIEVDDCPNAETIMKIVNYA
                     YKETNISYMGINFHIRYCKNCGTYLHNNECKCTKCGCQDIQGISRVTGYLSLDERFGP
                     GKYEEREDRRSHLEGNKKAYHCV"
     regulatory      5237..5336
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5450..6448
                     /locus_tag="K8M77_000395"
     CDS             5450..6448
                     /locus_tag="K8M77_000395"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBI7084923.1"
                     /translation="MNLLNLPKIELHCHLDGSLRVETAIELAKKEGIKLHSYEYDKVK
                     ELLVISKECNSLEDYLNRFALPAKLLQRPENLERVAFELMEDASKENVKYIEIRFAPL
                     LHLEKGMTQKEVIESVIKGIRKAEEFYDIKGNLILSCLRHHSIDSVYEVIEEGKNFIG
                     KGVVAIDLAGGELEGFVKPYKEVMKLARESGFRVTIHAGETGYGKNVRDAIELLGAER
                     IGHGLFIFNDEEAYNLVKEKGVTLEMCPKSNIDTKGVNKYEEHPIYKYHKDNIRVNLS
                     TDNRTVSNINLTEEFENVHKTFNIDFEDYKKIYLNSVEASFCSEELKEKLKLSIII"
     gene            6825..8216
                     /locus_tag="K8M77_000396"
     CDS             6825..8216
                     /locus_tag="K8M77_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7084924.1"
                     /translation="MKGFKNKEFLPTMIMMAIPITIQSFITSSLNLVDNLMVGKLGEE
                     SIASVGLANQYIFIFTLCILGINAGASVFMSQFWGKKDLKNIKKVLGLDISIGLIVST
                     LFALGSGFFSYEIMEILSNDLNVIELGAKYLQIVSISCIFTNFTQAYSSALRSTEQPS
                     VPMYASIVGVLINAFLNWVFIFGNLGFKPMGVNGAALATTIARLVEMIFIVSFVYFRK
                     NKVASRLNELISFDLNFVKIYFRTSWSVIVNEIVWSLGMTAYSIAYARIGTNAVASMQ
                     IATTLNNMFMVLLIGLATASSIMIGNKIGSNEEEIAREYAKDIAVIAVLVGFILGILI
                     WITAPLALKPFNVSEGTYLDTVKVLRIIGLFFTIRAFNMVMIVGVFRGGGDTTYSMLV
                     QCGTIWLYAVPMAFLGATVFSLPVYGVYFLICTEEFLKIIFEAVRLKSGKWIKNVISD
                     IEEDESDNNLITV"
     gene            complement(8274..8882)
                     /locus_tag="K8M77_000397"
     CDS             complement(8274..8882)
                     /locus_tag="K8M77_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBI7084925.1"
                     /translation="MTNQNLFLEIEKHLLEDAKPSIFLNKIKEKDSFKNSSFNIFNQL
                     NNENQINESNPWTHTLNVVDLASKYKDLSNNPKAFMWAALFHDIGKIRTLNPIHDRIG
                     GEITRRFLREYSDDTYLNEKIISLVKHHSAPIFIINRVSYSIIDLINTADIHDVALLS
                     LCNRLRNLSSKNNDLEKNLLDSNKFLKIMSSKTCLPLKEIIV"
     gene            8991..9500
                     /gene="nrdG"
                     /locus_tag="K8M77_000398"
     CDS             8991..9500
                     /gene="nrdG"
                     /locus_tag="K8M77_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBI7084926.1"
                     /translation="MSNTIRLSGIAYESLVNGPGIRRVFFSQGCKHNCIGCFNPDTHD
                     FNGGEERDIDELIKDLADNPFIDGVTFSGGDPLEQANKFIYIAKAARELGLNIWSYTG
                     YKFEYLLEHANEFNKWKEYLENIDVLVDGKFDINKKEDGLRFRGSSNQRIINVKESLK
                     EGKVIELEF"
     gene            9620..10171
                     /locus_tag="K8M77_000399"
     CDS             9620..10171
                     /locus_tag="K8M77_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBI7084927.1"
                     /translation="MLEFVLNKNEFVKCRKEFIIREIFFWTLFEIIGAIFVFIMTMVF
                     IKANKNLNNSFYFITLLAWFIFSIMCNIKNKKIIERDIEEKGRFLENKNMFGNCKVQI
                     LANGIRIEYNEGAVERIYPWKIIREVFSFKENIYLFISSIDYVSIPKGAFKNKEERIS
                     FLNKISNDRNNVRERILSIIGLA"
     gene            10317..11798
                     /locus_tag="K8M77_000400"
     CDS             10317..11798
                     /locus_tag="K8M77_000400"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBI7084928.1"
                     /translation="MYNFLVKKSKCTETDALLIPYFEEKTNIPNQEINNKINIIKKKE
                     QFKGSYGEIFNITRTTEDNIQDIILLGLGKETEITKEKIRRAFGKAVNEIKRLKSKSV
                     FLRFDSVEAIGLENTLKAMVEGLALGSYSFNKYKSDKKEEVEVTVHIGGHNIKEEEIE
                     FCEKVIDEAVLLSETTCLARDLVNEPANNMYPKTLAKEVKNIGSKYGFEVEVFDENQI
                     EELKMESFLSVGKGSDNLPRLIVMRYFGDKDNMDQRLALVGKGLTYDSGGYSLKTNAG
                     MVTMKSDMGGAASVIGAISAIAKRNLKINVIAVVAACENLISGHAYKPGDIIGSMAGK
                     TIEILNTDAEGRLTLIDAVTYAIEKEKASEIIDVATLTGAALVSLGEDVTAVITNKDE
                     FYGELREASYETGEKVWQMPSFEDYSKLIKSNIADLKNIGGKYAGTITAGLFIGEFVQ
                     NKPWLHLDIAGPAFSEKKGDYCPTGGTGAGVRTLYELANRRCK"
     gene            complement(11912..13309)
                     /gene="asnS"
                     /locus_tag="K8M77_000401"
     CDS             complement(11912..13309)
                     /gene="asnS"
                     /locus_tag="K8M77_000401"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBI7084929.1"
                     /translation="MKDVVLVKSLYRNTSEYSDKKVKISGWIRTLRASNAFGFIEVND
                     GSFFKNVQVVFDSAKISNYKEISKLPISSSISVIGTLVETPDSKQPFEIQAEEIIVEG
                     MSDSDYPLQKKRHTFEYLRTIAHLRPRSNAFSATFRVRSVAAYAIHKFFQDQGFVYTH
                     TPILTGSDCEGAGEMFRVTTLDMMAPPISEEGGIDFSQDFFGKETNLTVSGQLNAECF
                     ALAFRNIYTFGPTFRAENSNTVKHAAEFWMIEPEMAFADLIDDMEVAENMLKYVIKYV
                     MDECPEEIAFFNQFVDKGLLERLNHVVNSEFGKVTYTEAVKLLQESGKEFEYPVEWGI
                     DLQTEHERYLTEQIFKKPVFVTDYPKDIKAFYMRLNEDGKTVAAMDCLVPGIGEIIGG
                     SQREERLDVLKARMAELNLNEEDYWWYLELRKYGETVHSGFGLGFERLIMYITGMANI
                     RDVIPFPRTTGTAEF"
     misc_binding    complement(13374..13614)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13720..14631
                     /locus_tag="K8M77_000402"
     CDS             13720..14631
                     /locus_tag="K8M77_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084930.1"
                     /translation="MCNFFEEKFEQINKLKHLMTLYKGNNQAKIYKEQIEDIFKEFQR
                     DYIREESNTILILTKEDLANFNGENGNPAYISIDGIIYDISNIELFKQSPYNSLKLGS
                     DVTETFYELNDGDESILRDIPMVGLLAEPEEAEFMDRVEEYYPQQKLKIMSSEELKKY
                     NGENGRLAYVAIEGIVYDITNLSGLELFNGTNLRLGSDITKEFKTYYRGDKELLKDAK
                     EVAILHDFNESQRGKHIEHNLKELTLNELSKYNGEKGKPPYISIFDTIYDLSYVDKWQ
                     EKNIKVGYDLTGEYKEVYGNDKSHLKN"
     gene            complement(14811..15509)
                     /locus_tag="K8M77_000403"
     CDS             complement(14811..15509)
                     /locus_tag="K8M77_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBI7084931.1"
                     /translation="MRIAVISDIHGNLYALREVLEDIDNQNIDSIICLGDLVGYGPHP
                     NEVIALIKRRNILCLKGNYDASVVDNDYTYIRDTDINSFSLPWTVEELRTSNRYYLDN
                     LPDDISMNVCGKSIKFVHGSPRKINEYLSEDYSELEEIMDELKEDVLVCAHTHIPYAK
                     KIGDKVIINDGSVGKPKNGKPHATYVILDFSNEFKLKVEMKTVPYEFRRTMKDMELKN
                     FPQKLIYSYEFGKE"
     gene            15677..16753
                     /locus_tag="K8M77_000404"
     CDS             15677..16753
                     /locus_tag="K8M77_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBI7084932.1"
                     /translation="MKEGRVKRILEAMKANDIPQMLVSSPASIFYLTGKWISPGERMI
                     TLYLNTKGEQKFIVNELFPINEDLGVDILWYNDTMNPIDILDKIIDHEEVLGIDKDWK
                     AHFLIQLLDRNSAKKFVNGSPIVDRVRMRKDDEEIALMKEASRINDIVVEKAIKSLKE
                     GMTEKEVVEVLGKGYAEYGCQGYSFEPIVAFAANAADPHAESGEQKLEKGMGVLIDTG
                     CRKDYYCSDMTRCVFFGEPTEHQKEIFNTVLEANKKAIDMIKPGVRFCDIDKAARDVI
                     ENKGYGKYFTHRTGHSIGIETHDFGDVGSTNTDEVKQGMIFSVEPGIYLQGDMGVRIE
                     DLVLVTEDGCERLNHLKKELVVIK"
     gene            complement(16828..18054)
                     /locus_tag="K8M77_000405"
     CDS             complement(16828..18054)
                     /locus_tag="K8M77_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI7084933.1"
                     /translation="MDRFIGVIGLICIIGIAVLFSENRKKINWRLVGTGLLLQIIFAL
                     LILKVPAGRAVFEWISNGITKLLDFTKEGSSFLFGPLLDTDKFGMIFALQVLPTIIFF
                     SSLMSVLYHLGIVQVVVKVIAKGVAKALGTSGAETFSAVGNIFLGQTEAPLLVKPYIK
                     NMTKSEICAIMVGGMATVAGGVMAGYVAMGVNAGNLLAASIMAAPAGLILAKILVPET
                     EVPETKGGGTLDLKVESENVIEAAANGASEGLGLALNVGAMLLAFVALIAMINALFGA
                     IGGIFGAPWLSLNWILGRLFSPLAFIMGVPTKDVFVAGDLLGIKLAVNEFLAYSQLSN
                     YIANGTLEPKTIMILTYALCGFANLSSVAIQLGGIGGLAPEKKPTIAKLGFKALLGGV
                     LATCMTATIAGILFSA"
     gene            complement(18135..18953)
                     /locus_tag="K8M77_000406"
     CDS             complement(18135..18953)
                     /locus_tag="K8M77_000406"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBI7084934.1"
                     /translation="MDLSNKIKAAAEYIKGKSKYNPTLGLILGSGLGAIADQIEDAEY
                     FPYNEIPNFPVSTVEGHAGRLVIGKFQGKEVVAMQGRFHYYEGYSMQEVTCPVRVMRL
                     LGVETLVVTNAAGAVNKEYTPGDLMIISDHLNLSGSNPLIGKNLNEFGTRFPDMSNAY
                     DKDLRSQVKDIAKNLGIEVKEGVYAMFSGPTYETPAEVRMAKILGADAVGMSTVPEVI
                     IANHSGMKVIGVSCMTNMAAGILEQPLNHEEVMETSAKVRKTFIELMTNIIKEI"
     gene            complement(19068..20087)
                     /locus_tag="K8M77_000407"
     CDS             complement(19068..20087)
                     /locus_tag="K8M77_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7084935.1"
                     /translation="MSVTINNIAKEAGVSLATVSRVINNSGYVKKETRENVIKVINKY
                     NYTPSAIARSLSKSITNTIGVIVPDITNPFFGSIIKGISDVAEVHNLNLILCDSNESI
                     DREIKAIKTLKEQRIRGIIICPTSVENDLNSEYLKTITNLGIPVILIDGSLKYHNFNG
                     VFVDNIKGSYDAIEALINANHKDIAIITGRMTSKPAQDRLLGYEKALLMNNIPINNDL
                     IFYGNYEEESGYECTKKILAMKNRPSAIFVCNNLMTLGCLKALREAKLELSKDISLIS
                     FDNIPILDTLGINISHINGPTKELGEIGMDLLIESLNNDSKKELNSITITPELVLKGS
                     EKLIK"
     gene            complement(20297..21670)
                     /locus_tag="K8M77_000408"
     CDS             complement(20297..21670)
                     /locus_tag="K8M77_000408"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBI7084936.1"
                     /translation="MAFDLLKDINKKVHFIGIGGVSMSGLAAVLLNAGYKVSGSDSKE
                     SEITNRLKEEGAKIYIGHSKDNLQDVDVVVYTAAIPSDNPEIIKAKEDNLILMDRAEF
                     LGQIMKGHKFNVAVAGTHGKTTTTSMISHVALSADLDPTILVGGDLDIIHGNFRVGNS
                     EYFITEACEYKQSFLRFFPYVGIILNIDADHLDFYKDINHIKDTFKQFVKLIPNDGYI
                     IGNADDKKVMEVLEVAKCNVLTYGINNGDIQARNIEFNEKGCATFDVFRNEEKLLSLS
                     LNVPGMHNVSNSLSAVCLAEIFNINADAIVSGLSSFGGAHKRFEYKGTKNDITVIDDY
                     AHHPVEIKATLSTAKKMNHNKIVCVFQPHTYTRTKTLFNDFVKCFDDCDELVLMDIYA
                     AREKDLGEINSDQLGDAIRAHGVKCTNVHSHEEALEYVNANLSKGDLLLTVGAGDVVK
                     VGELFLN"
     gene            22030..22833
                     /gene="purR"
                     /locus_tag="K8M77_000409"
     CDS             22030..22833
                     /gene="purR"
                     /locus_tag="K8M77_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBI7084937.1"
                     /translation="MEKLSRNSRVSIITKILIENPNKIIGLNKFSDLLNAAKSTISED
                     IVIVREVLSKFSMGKVETIAGAAGGIRYIPEVGEMEKRKFLEELCDIASESNRVVPGN
                     FLYVTDIMFNPSIISKASIILASYFQGMDVDYVITVETKGVPLAYEVAKCLGVQLITA
                     RRDSKVTEGSTVSINYVSGTSGKIQQMCLSRKSLKQGSKCIFIDDFMRGGGTAKGLVD
                     LLKEFDSELMGIGVLIDNKLSTNKITKDYVSLIDIVEINEDGVKMVPSM"
     gene            22944..23216
                     /gene="spoVG"
                     /locus_tag="K8M77_000410"
     CDS             22944..23216
                     /gene="spoVG"
                     /locus_tag="K8M77_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBI7084938.1"
                     /translation="MNITDVRIRKISAEGKMKAIVSVTFENQFVVHDIKVIEGQNGLF
                     IAMPSRKTPDGEFKDIAHPIDTQTREQIQKAILDEYEKVKNLDMQE"
     gene            23427..24791
                     /gene="glmU"
                     /locus_tag="K8M77_000411"
     CDS             23427..24791
                     /gene="glmU"
                     /locus_tag="K8M77_000411"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBI7084939.1"
                     /translation="MNKCAIILAAGQGTRIKSKLPKVLHKACGKEMVNHVIDAMRNAE
                     IDDVNVIIGKGAELVKERTTSKNVSYSLQAEQLGTGHAVKCAKDFLEGKTGVVAIFTG
                     DAPLIKAETVKNLVDTHINEKNSATLLTSVIENPTGYGRIVRNGESVEKIVEHKDCNE
                     QEIKIQEVNAGMYCFDIESLLTSLEQLSNDNAQGEYYLTDVIEILKKENKKVGAMITD
                     FEETLGVNSRAELAKVESIMRNRINRTHLDNGVTIIDPLNTYIEPEVVIGKDTIIYPG
                     NVIEGKTVIGEDCILYPNSRINNSTIGNGVEIQSSVILDSKIGDETTVGPFAYVRPES
                     NIGEHVRIGDFVEIKKSTIGNNTKVSHLTYIGDAEVGERCNFGCGTVVVNYDGKKKHK
                     TIIGDDSFIGCNTNLVSPVEVKDNTYIAAGSTITKEVPEGSLAIARAKQQNIEGWVER
                     KKLK"
     gene            24848..25807
                     /locus_tag="K8M77_000412"
     CDS             24848..25807
                     /locus_tag="K8M77_000412"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBI7084940.1"
                     /translation="MENHSKNIKIFTGNSHPELAREIAKALNIPLGKAEVSTFSDGEI
                     SVNIKETVRGCDVFIVQSTCSPVNNNLMELLIMIDAFKRASAGRINAVIPYYGYARQD
                     RKAKSRDPITAKLVADLLTAAGADRVLTMDLHAAQIQGYFNIPVDHLLGSPILAKYFV
                     EKGLADRDDVVVVSPDLGSVTRARKFADKLNAPIAIIDKRRPKANVSEIMNIIGDVKD
                     KVCILIDDMIDTAGTITNAANALKDLGAKNVYACCTHGVLSGPAFERINNSAIEELVM
                     LNTIALPEGDGLNKFKSLSVAPIMADAINRIYDDEPLSGLFQD"
     gene            26073..26759
                     /locus_tag="K8M77_000413"
     CDS             26073..26759
                     /locus_tag="K8M77_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7084941.1"
                     /translation="MEGLLGKILIVDDDENICEVIKMYLETTGYNVKVAHDGKAAKEE
                     FVNFSPNLVVLDMMLPGIDGMEVLKWIRKDSNVPVIMLTAKGETFDKVLALEIGADDY
                     VVKPFEPKELLARVKAVMRRYSGEEPDNVVLNFPGLTIDANSYKVIYNGEEVKTPPKE
                     FELLHYLASNKNKVFTRDQLLCEVWGYDYPGDSRTVDVHIKRLREKLNGGEEWQLETV
                     WGVGYKFEVK"
     gene            26759..28168
                     /locus_tag="K8M77_000414"
     CDS             26759..28168
                     /locus_tag="K8M77_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBI7084942.1"
                     /translation="MRYRGLVPKLILTLSVTICITFFIVGIILSSLFREEYYQERLKT
                     LSGEAEVIEQVVNSFMNSETTLVELDKSISFIGDSLKADIYLIDSYGYIYSVSNEKYD
                     YLKRTKINDDDMKILKENTPIEQKKKNEFIYSKPIFCKGVFTGAVSIVTPLSTLSDSL
                     VRINQIVWMCVAIAIIFSGLSIYFISKKIIIKPLKVINNAAKKIANGEIGNRVNVSYD
                     DEVGELAESFNIMATSIEEAELNRREFISNVSHELRSPMTSIKGFITAILDGVIPKDK
                     EEYYLVIVNDEISRLTRLINDLLDLSAMQAGKLEFNISELELNRIIETTVLKMNQKSA
                     SKNIKIEVLLESEKLYVYGDNDRLIQVVTNLVDNAIKYCDENGEVKISTKTKGSKIIV
                     SIFNTSKGIDEYDLTHIWDRFYKVDKARSNKTSTGLGLSIVRNIILQLEEDIWAENVK
                     DKNGEIKGVRFNFTLTKVK"
     gene            28189..29346
                     /locus_tag="K8M77_000415"
     CDS             28189..29346
                     /locus_tag="K8M77_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBI7084943.1"
                     /translation="MGNFFKNSKKGKNLHGVNEKNYDNIVIKKDKKRFWIKSLLKLVS
                     FVILVVLVSFIAFKHYTKDLNMKEQKANLLNEIKDGRYDITNLVNKSGCSLVTIGDDS
                     NKLAIGKDDGKNVSGSIIRTDGYIITSYSAIKDFSKIYVKISSCDISPFEAKVVGFDK
                     DTDIAVLKIGANGLKSISTSELEVLEVGEKVAALGNTISEEPVGFVSNGIVSSPIKKI
                     SIGEEGHSDSKVFDLIETNSLINSDNNSGILCDYNGIVIGINSLYFTNKFSKPGVYYA
                     LEINDALRIADSIIIKGGVTASVTLGVTGSTFADEKSNIYGIYVEDVDKDSNAFNSGI
                     KPTDIIVEADNKKIKDIENLADLLKKHSVGDIIKLKVIRGDITKDISVTLN"
     gene            29472..30038
                     /locus_tag="K8M77_000416"
     CDS             29472..30038
                     /locus_tag="K8M77_000416"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI7084944.1"
                     /translation="MILIVGLGNPGKQYEKTRHNIGFDVIDYMANKYNIDVNREKFKG
                     ICGEGFIENKKVILLKPLTYMNLSGESIREFANFYKLEDDEIIVVYDDISLDIGRLRI
                     REKGSAGGHNGIKSIIQNLGGDKFPRVKVGVGQPKDNLVNHVLGKFSKEDREHIEKVI
                     PVVSDAIVEIVKNDAKESMNKFNGVNIE"
     gene            30069..33575
                     /gene="mfd"
                     /locus_tag="K8M77_000417"
     CDS             30069..33575
                     /gene="mfd"
                     /locus_tag="K8M77_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBI7084945.1"
                     /translation="MRLQGLMAPLLNSEAFQKVKSFMESGNYPVMINGLSDSGKSYFI
                     NGIYEESDKPIVVVTHNDIEARNIYEDLSFYLPNVYYLPSREIVFYNIDAISGDLRWA
                     RLKVIKEMLRSTKKIIVTSIDAFVATYTPKELYKSHILKIKVGDEVDFKEISHKLIES
                     GYERLETVEGKGEFSLRGGILDVFPPNSANPFRIELFGDEVDSIRTFNVESQRSIEKV
                     KRAEIFPAKEVILSKETIEHAIEKMRKELSDFENKVSDKEIKDKLRKLIEKNIESLQE
                     NWSFETIDSYLPFFFDKPATLFDYLNNYTFIIDDAKRCKGKLESTVFEFTENYEAFLE
                     RGSILPSQINLSLSAEVVEESFKRNKVINLVPFGDGGFIESFNKVEVKQSTKSGYQGQ
                     LDMLIDDIKENKKNGFRTLILSGTRPRGERLVNTLRELEIESVYKDEVDEINNGEVVI
                     TFGEQNKGFEYPDIKLCLISDKSVFGEGKRVKKAKRKKSKGVSKIKSFAELKPGDYVV
                     HVNSGIGVYKGIKQIEVQGHKRDYLDIEYSKGDKLYVPVEQLDLVQKYIGSDSASPKI
                     SKLGGNEWQKAKAKVRKSINDIAGDLVKLYAERSTVKGYKFSKDTQWQKQFEDEFPYE
                     ETPDQLAAIEDIKSDMESNKVMDRLLCGDVGYGKTEVAIRAAFKAVMEGKQVAFLVPT
                     TILAEQHYKNMKRRFAGFPITIDMISRFRTKKQQTETLKALKEGNVDILIGTHRLVSK
                     DIQFKDLGLLIVDEEQRFGVAQKEKIKSFKKNVDVLTLSATPIPRTLHMSLTGVRDIS
                     VIETPPENRYPIQTYVVEQNDQLIRDAILREINRGGQVYFVHNRVESIQEVANYIQEL
                     VPECKATVIHGQMTERQLETEMIDFMERKYDVLVCTSIIETGIDISNVNTMIVNDADK
                     MGLSQLYQLRGRVGRSNKVAYAYFLYQRDKVLTEVAEKRLKALKDFTELGSGFKIAMR
                     DLEIRGAGNMMGSAQHGHMAAIGYDLYCRMLEDTIKIVKGEIEREPVETTVDLKVDAF
                     IPSSYIKDEIQKIEVYKKIAAIENEEDYEYVREELEDRFSNIPNTVYNLMDIAYIKSK
                     AKLLNIEEIKEKNEDIIFIFESRERTDKRIFKILLEEYKDDILIEFGDKPTILYRVKD
                     KKKEDVLSIFKEMLDKLIALNN"
     gene            33676..34704
                     /locus_tag="K8M77_000418"
     CDS             33676..34704
                     /locus_tag="K8M77_000418"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI7084946.1"
                     /translation="MVSVKKIVASALVGVLMFSAVGCNMVEKTQAAIDKTTVATVNGE
                     KITLGEVDSNLKGVFTKMKSQYGDKYMDDPQVAQQILQQRQSVVQSLVTDKVLGIEAD
                     KLGIKPSEEEIKKKVDEQFENIKKGMGDNFDKALEGEGYTEDTFKDVIKNQVINQAVQ
                     DYVIKDVKVTDEDAQKYYDENKQQFVAKESGVLTKHLLFENEEEAQKAYDEIQSGKTT
                     FNDLFTKYENNKSENKKPIAENLGVVPAENSELVQEFVDGLKPLKEGEISKPIKTQFG
                     YHIIQAGATYEKGAQLPFDEVKSQIIQILKQQKDSEKFKADMDQWKKDLNVKVYDDKL
                     QEGLKISK"
     gene            34899..35447
                     /gene="spoVT"
                     /locus_tag="K8M77_000419"
     CDS             34899..35447
                     /gene="spoVT"
                     /locus_tag="K8M77_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBI7084947.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIFTDRDGGV
                     ILKKYSPIGELTDFSKEYAEALQQSTGHIILISDNDAFISVSGTSKKEFLERKISDEL
                     EDIMDKRKSVVLSATNKNLIPIHGDDDESKYSGQVIAPILAEGDAIGSVVILSKEGKA
                     LGEVEQKLAETAAAFLGKQMEQ"
     gene            35640..37172
                     /locus_tag="K8M77_000420"
     CDS             35640..37172
                     /locus_tag="K8M77_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBI7084948.1"
                     /translation="MKKQSLIKGSLVLACAGILAKFLGLFFRWPLIMLIGDEGLGYYQ
                     MTYPLYMLFVAIAAGIPIAVSKLVSEKNALGKYEESFQVIKEALKVMVLLGIGTSIFL
                     AVGGKFLLPFLKWDDKAYYSILGICLAPTLIGIITPIRGFFQGFQNMTPTAISQILEQ
                     IGRVIVGVGLAYILIDKGIEYSAGGASFGAVAGAFVAALYLIPKYRKIKNSFKLGKIK
                     GSHKIFMEIINIALPISIGAAVGSVMGVIDSVLVPQKLLQAGFTGQEATILYAQLTGK
                     ATVFTNIPMTLATALAASIIPIISELYILKDKVQVKQKIDTTMKLCMIVSIPCFLGLF
                     FMAEPIMNLLFPGKADGFMILKYLSISVPFLVGTQITTSILQGMGYYYRPVFHLFIGC
                     ILKVLLTMNLIPIRSLNIYGAVIATIISYSVVLILNLLYMAKRFKYAPNIKDVIIKPI
                     FASILMIGTVLYLYTQALKYSESNSISCLIAVFVGIIIYMISLFVLKIFSVEEINGRL
                     RN"
     gene            37203..38654
                     /gene="mazG"
                     /locus_tag="K8M77_000421"
     CDS             37203..38654
                     /gene="mazG"
                     /locus_tag="K8M77_000421"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBI7084949.1"
                     /translation="MLKIMGLGPGAYEALTIGALKELKNNKNIYFRTEKHPTVDFLKD
                     EGIKFESYDHAYEKYDSFDDVYKYIAEDLITKIKDDEDLIYAVPGHPLVAEKSVINLI
                     ELCKENNVQYEVLPAVSFVDAMMEALQVDPIEGVKIIDAFDMKNQILDKRIGTIITQV
                     YNKFIASEVKLRLLEGYEDDTEIIFVRAAGVEGLESIRKIPLYELDWQEDIDYLTSIY
                     IPKDLGNKKDFQDLLDIIETLRNPGGCPWDREQTHESLKSALLEECYEVIDAIENEDE
                     DALIEELGDVLLQVVFHASIGKEDGYFDIMDVIGGISNKMINRHPHVFGNEEANTSEQ
                     VLVNWDEIKKEEKGIKTLTEEMQNIAKSLPATTRAYKVQKKAKKVGFDWNDVNYAMDK
                     VKEELNEIKEVYNCEDKSIIEGEVGDLLFACINVARFLEVDGELALDKTIKKFIKRFS
                     YIENEAIKNNKNLKDMTLEEMDKLWEEAKTSEK"
     gene            38871..39146
                     /locus_tag="K8M77_000422"
     CDS             38871..39146
                     /locus_tag="K8M77_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBI7084950.1"
                     /translation="MNKAELITSMAEKSNLTKKDAELALKAFIESVSETLENGDKVQL
                     VGFGTFETRVRAEREGRNPRTKEVIKIPASIAPVFKPGKELKERVNA"
     gene            39254..39514
                     /locus_tag="K8M77_000423"
     CDS             39254..39514
                     /locus_tag="K8M77_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBI7084951.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEACESGRVIINGKVAKPSNDIKEE
                     DIIEILFGNRTLKARVINIADHVRKENAKEMYEILEGEEDKE"
     gene            39634..39933
                     /gene="yabP"
                     /locus_tag="K8M77_000424"
     CDS             39634..39933
                     /gene="yabP"
                     /locus_tag="K8M77_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBI7084952.1"
                     /translation="MTDKKSENKTIQGASSLSLENRKILSITGVNEVINFDEEKISLK
                     TDLGPLLIKGSELKMNKLDVQNGEVIIGGFISNIDYLNKKNKRKRNINLLSKIFK"
     gene            39943..40317
                     /gene="yabQ"
                     /locus_tag="K8M77_000425"
     CDS             39943..40317
                     /gene="yabQ"
                     /locus_tag="K8M77_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HBI7084953.1"
                     /translation="MLLDINIQFKIVIFAILSGIIIGFLFDIYREFRGVCKNKILITI
                     EDILFWIWCSLIVFVFLLKYNYALIGVYVYVFMGITLVIYLKTLSKYFRRIQHKILAI
                     LLKNLRIISKNIRYAFKNTFTN"
     gene            40413..40733
                     /locus_tag="K8M77_000426"
     CDS             40413..40733
                     /locus_tag="K8M77_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBI7084954.1"
                     /translation="MKKRMNLKNMVIIILIAILVISYVRQELTMNKIHKDIEAKQTQL
                     NELQDKNKKLQDEVNQSSTDEYIERMARERLGMIKDGEKVITNSDSPQDKQNNSEVKN
                     NQNK"
     gene            40793..41197
                     /locus_tag="K8M77_000427"
     CDS             40793..41197
                     /locus_tag="K8M77_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBI7084955.1"
                     /translation="MTLMAGNILEGRIINITNFGAFVEVEGKTGLVHISEVADTYVKD
                     IREHLKEDDKVKVKVISVDDKGKISLSIKQAMPPKKKSVKPADFDWNNEKKAKPKQNF
                     EDIMSKFLKDSEERLQDVKKHQDVKQRKRKSI"
BASE COUNT        15938 a         4793 c         7070 g        13793 t
ORIGIN      
        1 aatctagtaa ttctcggaat atgtttgtaa gttttaaata agttattttg atacatgccc
       61 ttttttagtt ataaagatgt gaatatatta ttttaaaaac ttccaaggct tcttttattt
      121 taggttttaa taagtgtttt ttttcttcaa actctttaaa accttctggc gttaaagaat
      181 atactttttg tatttttctt ttaggatcat cccaagagcc agttacaaag ccttgttttt
      241 ctaatttctt tagtaaggga tatattccac ctgtgctagg aaaccaaagc ccattagttt
      301 gttcaccaat tttctgagct aaatcgtttc cgttagtagg ttgaatacta agaatatata
      361 atgcatatat aggaagaagg cccttagtaa agacttgtcc tacagcatct ttttcttttt
      421 gtactttttt taaattggct atctgttttt tatattcatc ataaaggcgt ttttcttcag
      481 cttttataaa ttcatcatta tacatagcta aatttccttt tgaattgaaa cacccaccat
      541 accagggcca gcatgaactc ccatagcagc tcctatagct cttacttgag attttaataa
      601 gttaggatga gaacttatta aattaagaac ttgattggct tcctcttctg ccataccatt
      661 aaggatagct acatagcatt tattttcatc aagatgctct aaaattatgt tagcaagctt
      721 tttcaaagat tgttttcttc ctcttgcttt agcaattgtt gtataagcac catgttcatc
      781 aactgatatt attggtttaa ggtgaagtaa atctccaact gttcctgcaa ccttacctat
      841 tcttccacca cgtcttaaat attctaaggt attaagagta taaaatccgg ttgtagcttc
      901 tcttatactt ggaagagctt caattatttc ttcaaagctt tttccttcct taacaaattc
      961 agctgctttt ataaccatag aacctaaagg ataacctatt attttactat caaataagta
     1021 atatttaaaa gaactacgtt cttcaataac taatctagca gcattgaacg tacctgataa
     1081 tgagttagat ataaatatac ctattaagtg agtatagcct tcagcttcta atttatctaa
     1141 tactgaattt atatagtcag gactacatag agaagttgtt ggcacttcat tatgtaaatt
     1201 ttcgtaaaca tcaactggtt ttatatcaaa aatatcatta tattctctat ctttatatat
     1261 tatctttaaa ggtaatatat ttaagttata atcttttatt tcatctatag ttaagtcaca
     1321 agagctgtct gttattatag caattttttg cattttatat gcccccaatc attttatact
     1381 tactaaataa aataataatt tatattctat ttagtgaatt aaatagcaaa ccgaataatt
     1441 caacatttat agaaattaat gagaattaag tttagcttta actgaattat atatcaatac
     1501 tgataccatt atcaatattg ataatggtac aaagagagaa taatctgtta ataatatatt
     1561 aaaacagatt attctttaag aaaacggttt acttatgaac ttaattgagg gtttaattga
     1621 aagataaaga agaattttta tctactatat tccaaattgt gttacctcta gataagtgaa
     1681 ttggagtgcc atcttcagtt tttaatgtgg ttttactatt agagtcttct ttttcccaat
     1741 gaattttttc cattcttcca ttagagaaaa catatccttc tccagtacct gttaaatcaa
     1801 tatctaaatg agtattatca gattgaagtg acatatcagt tatttgtacc actatatttt
     1861 taaatttaag agacgagctg gtatttttat ctatggcaag ctctccatcc atatactttt
     1921 cataaaatcc atctacatat ttataagagg ttttataata gtcacttaga ttaagtttta
     1981 tttcagatat atagttagaa gaatttaata gagaattatc tttaaaactt aatgtattat
     2041 ttttagaata ctcatatcca ttatcagcaa tgaactttct tatattttta gctgaagtat
     2101 aaagattgtg aggggctttt cttgtgttat ctctccaaaa agcacttccg tttttaattt
     2161 cgtttatact atttaaggat ttgtcactag atattgtagc tagagcttct gcacttccac
     2221 cacaatgtgc aaaaggtagg tcgtgttcct tagaaatatc taaaaaataa ggacgtacac
     2281 ttcttacagg cccaatagct tcaggagaat tttcataaaa tacagctaag aatctaggaa
     2341 ttcctgcctc ggccatggtt tcataaacta tatctgcttc tgaaagtcca ctttgaggac
     2401 gagctgctct agaattttca attatacaca taaaaggaat attatctatt gaatctgatt
     2461 ctaataagtt tcctgtgtat gggctaaggg tttctaaaga taactcttcc atatcaaagt
     2521 catagggatt ttcttcattc attgttaaat ctattggctt tagttctttt aaggtatcta
     2581 ttttttctga tgtttttaaa ggcataatta aatttccaaa tatgaatcca actaataaac
     2641 ttaatattaa ttgaaaagca attaatgcta ttttcttcat ttaaactcct cctatgaatt
     2701 acttttcata tatttattat gttacattat atggaatatt attcatctta tggagaagat
     2761 aagaataagt ttttgccaat gtcaactaaa gaaaaattga atataggcta agaaaaagaa
     2821 ggaaagaggg agataataag tctttttttt agtataagga ttttttaaaa ttagcataac
     2881 tattaatagt agagtataat gattcgagta caagatatag tggagtggag gaattgaaaa
     2941 tgctacatgt tgtgaaaagg gacggtagag aatctaggtt taatgcggat aaaatagcaa
     3001 atgcaataaa gagagcagcc aatgaagttg gtattgcaat aagggaaagc caagttttag
     3061 atactataca aaaggtaatt aattatatag aaatgtcagg taagacagat gtcactgttg
     3121 aagaaattca agttttagta gaaaaggctt taatggataa tggttttaac caagtagcaa
     3181 cagcatataa ggcatataga actgaaagaa ctaaagttag agaaataaaa tcagacttaa
     3241 tgaaagctat aagtaagatc ggagtagaga ctgatagaga taatgccaat gtaggaaata
     3301 acttctcatc aaagctttta agaatagctt cagaatctaa caaatggtat aatttagcta
     3361 caatgcctaa agaattagca aaagctcatg aaaatggaga tgtgtattat catgacttag
     3421 atagttataa cttaactact aactgcttac acataccaac tggtgagtta ttacaaagtg
     3481 gatttaacac tggatacgga actattaatg caccaagaag aatagagaca gcagcagaat
     3541 tatcatgtat tttattacaa tcaactcaaa atgatatgtt tggaggacaa tcacatccag
     3601 actttgataa tgatatgagt gtttttgtag aacctacaag acaagagata agagaagaat
     3661 acataggatt aggcttagaa ggagctaaat tagatgaact agtagagaaa aaattaagag
     3721 aaagagtgca tcaagcaatg caaggagttg tttataattt aaacactatg cattcaagag
     3781 caggatctca agttcctttt agttcattaa acttaggaat tccatacaat gaagatgctg
     3841 ctttagtttg tgaaatattc ttattagaat acgaaaaagg attaggaaga ggagaacaac
     3901 caatattccc taatataatc tttagagtaa aagaaggtgt aaatagagaa gtagaggatc
     3961 catatttcta tttatatgaa ttagcttgta gagtagcagc taagagaatg aatccaactt
     4021 tcatgaatat cgatgcggac tttaataagg aatactatga taagggatat atgccagcaa
     4081 ctatgggatg tagaacttat cttatgaaaa atgttaatgg tgaaccagga tgtaaaggaa
     4141 gaggaaatat agctcctaca actataaacc ttcctagact tggaatatta gctaaaaaag
     4201 atattaataa attctttgat ttattagatg atagattaag aatagctaaa gaatctttaa
     4261 tgcacagata tggggttctt aaaaacttaa aagttaagga tttgcctttt gttgcaggtc
     4321 aaggattaat gaaaggtgct gaaaacttag gcccagatga ttcaatagaa ccaattttaa
     4381 aacaaggtac ttggggaatt ggatttatag gaattgcaga aactttaata gccttaactg
     4441 gaaagcatca tggtgaagat gaaacttcaa gagaattagg aataaaaata gttgagagaa
     4501 taagagaata ttgtgataga ttaactgagg aaactagatt aaactggagc tgttatgcta
     4561 ctccagcaga aggattatca ggtaaattta taaagcaaga taaaaaagcc tttggaataa
     4621 ttaaaggagt aacagataaa gattactata caaatagtta ccatgttcct gtttactgcc
     4681 caatgtcaat aaaaaataag atagatattg aagcaccata tcataaatta tgtaatggtg
     4741 gacatatcag ttatatagaa gttgatgatt gtccaaatgc agaaactata atgaaaatag
     4801 ttaactatgc ttataaagaa actaatataa gttatatggg aattaacttc catataagat
     4861 attgtaaaaa ttgtggaaca tatcttcata ataatgagtg caaatgtact aaatgtggat
     4921 gccaagatat tcaagggata tcaagggtta ctggatactt aagtcttgat gaaagatttg
     4981 gtccaggaaa atatgaagag agagaagata gacgttctca cttagaagga aataaaaaag
     5041 cttatcactg tgtttaatat tttcaatgaa attatttata taagtttata gaaaaatcta
     5101 tgcaatatat taaattttta tcttaattag ataaaaattt aatacatgtg catagatttt
     5161 tatttttact ttgaaaatca ttcaaacttt ttttaaaaat ttattgttga ctataaattt
     5221 ttatatggta aaataaataa aaaaataaat tttgcttcgt ataactctaa tgatatggat
     5281 tagaggtctc taccaagaac cgagaattct tgattacgaa gaaagcttat ttgctttctt
     5341 cgtaatcaag aatttttttg aaccttttta ggatatataa attctttatt caacattaga
     5401 aattttaatt ttattcataa gataaatggt taattaggag gaaatataag tgaacttatt
     5461 aaatttacca aagatagaat tacattgtca tctagatgga tctttaagag tggagactgc
     5521 tattgaatta gctaaaaagg aagggattaa actacatagc tatgaatatg ataaggtgaa
     5581 agaattacta gttatttcta aagaatgcaa ttctttagag gattatttaa atagatttgc
     5641 tcttccagca aaactcttac aaagacctga gaatttagaa agagttgcct ttgaacttat
     5701 ggaagatgca agcaaggaaa atgtgaagta catagaaata agatttgctc cattattgca
     5761 tttagaaaag gggatgactc aaaaagaagt aatagagagt gtaataaagg gaataagaaa
     5821 agctgaagaa ttttatgata taaaaggtaa tttaatatta tcttgtttaa gacatcattc
     5881 tatagatagt gtatatgaag taattgaaga gggaaaaaac ttcataggaa aaggtgttgt
     5941 tgccatagac ttagctggtg gtgaattaga aggttttgtt aaaccttata aagaagtaat
     6001 gaaattagca agagaatcag gttttagagt tacaatacat gctggtgaaa ctggatatgg
     6061 taaaaatgtt agagatgcca tagagctttt aggtgcagag agaatagggc atggcttatt
     6121 tatatttaat gatgaagagg cttataattt agtaaaagaa aaaggcgtaa cattagaaat
     6181 gtgtcctaag agcaatatag acacaaaggg tgtgaataaa tatgaggagc atcctattta
     6241 taaatatcat aaagataata taagagttaa tttaagtaca gataatagaa cagtttctaa
     6301 tataaatctt actgaagaat ttgaaaatgt acataagaca tttaatatag attttgaaga
     6361 ttataaaaag atttatttaa atagtgttga agcttccttc tgttcagaag agttaaaaga
     6421 aaaattaaaa ttatcaataa ttatataaat taaaaataat ccacaggtaa atagaactta
     6481 acttacctgt ggattatttt ttaatttaat aattgtggat atttttcaaa aaattattct
     6541 gtggataaat ttttaaacgt attattgtta gtaatttggt gtaatttcac taaaaacttt
     6601 agtgagatat atattataca ataaaacctc agtaaaattc cagtaaaaat cttataattt
     6661 taaatttttg agcttaaata ttttaaagaa atgtaaataa cagaaagatt atattgacta
     6721 agaggtatat ttattagaca attaatagta tttaaaagca gatgggcggc tactacccat
     6781 ttgctttaat tttttgtatt tttttaaaag ggggattaaa tttcatgaaa ggttttaaaa
     6841 ataaagaatt cttacctact atgataatga tggctattcc tataacaatt cagagtttta
     6901 ttacatcatc attgaatcta gtggataatt taatggtagg aaagctagga gaagagtcaa
     6961 tagcctcagt tggattagct aatcaatata tatttatttt taccttatgt attcttggga
     7021 taaatgctgg agctagtgtt ttcatgtctc agttttgggg caaaaaagat ttgaaaaata
     7081 ttaaaaaagt tttgggacta gatatatcta ttggacttat agtttcaact ctttttgcct
     7141 taggttctgg atttttttct tatgaaatta tggagattct ttctaatgat ttaaatgtta
     7201 tagaattagg agctaaatat ctacagattg tttctataag ttgtatattt actaatttta
     7261 ctcaagctta ttcttcagct ttaagaagta cagagcaacc tagtgttcct atgtatgcaa
     7321 gtatagttgg agtcttgata aatgcatttt taaactgggt atttatattt ggaaatttag
     7381 gatttaagcc tatgggggtt aatggcgcag ctttagcaac cactattgca agattagtag
     7441 agatgatatt tatagtttcc tttgtttatt ttagaaagaa taaggtggca tcaagattaa
     7501 atgagcttat atcctttgat ttaaatttcg taaaaatata ctttagaaca tcttggtcag
     7561 ttatagtaaa tgaaatagta tggtctttag gtatgactgc atattcaata gcctatgcta
     7621 gaataggaac aaacgctgtt gctagtatgc agattgctac cacattaaat aatatgttta
     7681 tggtattact aataggttta gctacagctt catctataat gattgggaat aaaattggtt
     7741 ctaatgagga agaaatagca agagaatacg ctaaggatat agctgtaata gctgttttag
     7801 taggatttat tttaggaatt ttaatatgga taacagcacc attagcatta aagcctttta
     7861 atgtaagtga aggaacttat ttagatacag ttaaagtttt aagaataata ggattgttct
     7921 ttacaataag agcttttaat atggttatga ttgttggtgt atttagaggt ggtggagata
     7981 ctacttactc tatgttagta caatgtggaa caatatggct ttatgcggta ccaatggctt
     8041 tccttggagc tacagtattt tctttacctg tatatggagt gtatttcttg atttgtacag
     8101 aggaattctt aaagataatc ttcgaagcag taagattaaa atcgggaaaa tggataaaga
     8161 atgttataag tgatattgaa gaagatgaat ctgataataa tttaattaca gtataaataa
     8221 aaaaggttat attaatagtt tcaaaataag attctattaa tataaccttt tatttatact
     8281 attatttctt ttaatggtaa gcaggtttta ctagacatga ttttaagaaa tttattacta
     8341 tctaaaagat ttttctctaa gtcattattt ttagaactta ggtttctcag cctattacat
     8401 agtgataata aggcaacatc atgaatatct gccgtgttta ttaaatctat tatgctataa
     8461 gagactctat taataataaa tataggtgca gaatgatgct ttactagaga tataattttt
     8521 tcgtttaagt aagtatcatc actatattct ctaaggaacc ttcttgttat ttctcctcca
     8581 attctatcat ggattgggtt tagagttctt atttttccaa tatcatggaa taaggctgcc
     8641 cacataaaag cttttgggtt attagataaa tctttgtatt ttgaagctag gtctactaca
     8701 tttaatgtat gggtccaagg attagattca ttgatttgat tttcattatt aagttggttg
     8761 aaaatattaa aagaagaatt tttaaatgag tctttctctt taattttatt taagaatatg
     8821 gatggcttag catcctctaa taaatgtttt tctatttcta aaaataagtt ttgattggtc
     8881 attttgtacc ccctaattaa tacttatttt aatgattaac aatagttagc atttttaaac
     8941 agataatttt aaaaaaagga acatataaat aatataggag gtaattttct atgagtaata
     9001 ctattagatt atcaggaata gcctatgaga gcttagtaaa tggtcctgga ataagaagag
     9061 ttttcttttc acaaggatgt aaacataact gtataggttg cttcaatcca gatactcatg
     9121 attttaatgg tggagaagaa agagacatag atgaattaat aaaggattta gctgataatc
     9181 catttataga tggagtaaca tttagtggtg gagatccatt agaacaagct aataaattta
     9241 tatatattgc taaagctgca agagaattag gattaaatat ttggagttat acaggttata
     9301 aatttgaata tctattagaa catgctaatg aatttaataa gtggaaagaa tatttagaaa
     9361 acattgatgt tttagtggat ggaaaatttg atataaataa aaaagaagat ggattaagat
     9421 ttagaggttc ttcgaatcaa agaataataa atgttaaaga aagtctaaaa gaagggaaag
     9481 taattgaatt agaattttaa gactaaaatt tcaattgtaa tatcttcata atagtttatt
     9541 taaaaaagct atgataaaat tatttatata aattttggaa aattaaatat taaattctta
     9601 aagggagtag aaagcgaata tgttggaatt tgttttaaat aaaaatgaat ttgtaaaatg
     9661 tagaaaagaa tttataatta gagaaatatt cttttggact ttatttgaaa ttataggtgc
     9721 tatatttgta tttataatga ctatggtttt tattaaagct aataaaaatt taaataacag
     9781 tttttatttt attacattat tagcatggtt tattttttct ataatgtgta atattaaaaa
     9841 taaaaaaata atagagcgtg atatagaaga gaaggggaga ttcttagaaa ataaaaatat
     9901 gtttggtaac tgcaaagttc aaattttagc aaatggaata agaattgaat ataatgaagg
     9961 ggcagtagag agaatatatc cttggaaaat tataagagag gtattttctt ttaaggagaa
    10021 tatatattta tttatatctt caattgatta tgtttcaatt ccaaaaggag ctttcaaaaa
    10081 caaagaagag cgtatttctt ttttaaataa gataagtaat gatagaaata atgttagaga
    10141 acgtatatta tctattattg gattagcata agcttaactt tttagttaag ctttttttat
    10201 attgatttgg taatctaggt tacatatttt tttataaatt aggtatatat taaagataga
    10261 ttattaaagg taagtgtgat atattaatta taaaaattta aagaggtgga aaataaatgt
    10321 ataatttttt agtgaaaaaa tcaaaatgta cagagacaga tgcattatta atcccatatt
    10381 ttgaagaaaa aacgaatata cctaatcaag aaataaacaa taagataaat ataattaaga
    10441 aaaaagaaca atttaaaggc tcttatggag aaatatttaa tataactaga acaactgagg
    10501 ataatataca agatattata ttattaggtc ttggaaaaga gacagagata actaaagaaa
    10561 aaattagaag agcctttggt aaagctgtaa atgagattaa gagattaaaa agtaaaagtg
    10621 tatttttaag atttgattca gtagaggcta ttggcttaga aaatacatta aaagcaatgg
    10681 ttgaaggatt agccttagga tcatattcat ttaataaata taagagtgat aaaaaagaag
    10741 aggttgaagt tacagttcat attggggggc acaatataaa ggaagaagag atagagtttt
    10801 gtgagaaagt tattgatgag gctgttcttc tttcagagac tacttgctta gcaagggatt
    10861 tagtaaatga acctgctaat aatatgtatc caaaaacttt agctaaagag gttaaaaata
    10921 taggttctaa atatggattt gaagttgagg tatttgatga aaatcaaata gaagaattaa
    10981 aaatggaatc tttcttatca gtaggaaaag gatcagataa tttacctaga cttatagtta
    11041 tgagatactt tggagacaag gataatatgg atcaaaggtt agccttagta ggaaagggac
    11101 taacatatga ttcaggtggt tattctttaa aaactaatgc tggaatggta acaatgaagt
    11161 ctgatatggg tggagccgct tctgtaatag gagcaataag tgccatagct aaaagaaatt
    11221 taaaaataaa tgtaatagcc gttgtagctg cttgtgaaaa cttaatatca ggtcatgctt
    11281 ataagcctgg ggatattata ggttcaatgg caggaaagac tatagaaata ttaaatacag
    11341 atgctgaagg aagacttact ttaatagatg ctgtaactta tgctatagaa aaagaaaagg
    11401 ctagtgagat aattgatgtg gctacattaa ctggcgctgc tttagtttcc ttaggagagg
    11461 atgttacagc cgtaataact aataaggatg aattctatgg agagctaaga gaagcttctt
    11521 atgagactgg agaaaaggta tggcaaatgc ctagctttga agattacagt aagcttataa
    11581 aatctaatat agcagattta aaaaatatag gtggaaagta tgcaggaaca ataactgcag
    11641 gattatttat tggagaattt gttcaaaata aaccttggtt acacttagat atagctggcc
    11701 cagcattctc agagaaaaaa ggagattatt gtccaactgg aggaacaggt gcaggagtta
    11761 gaactttata tgaattagct aatagaagat gtaaataaga taaaaagtaa gaattaagat
    11821 tataaccttt attaatttta aaagctaata gttaataaat aagaatttat tttataaatt
    11881 aaaaaagaag tggattattc cacttctttt tttagaattc tgcagtacca gtagttcttg
    11941 ggaatggtat tacgtctctt atgttagcca ttccagtaat gtacattatt aatctttcaa
    12001 aacctagacc aaatccagag tgaacagttt caccgtattt tctaagttct aaataccacc
    12061 agtaatcttc ttcatttaag tttaattcag ccattctagc ttttaagaca tcaagtcttt
    12121 cttctctttg actaccacct atgatttctc ctattccagg aactaagcag tccatagcag
    12181 caactgtttt tccgtcttca tttaatctca tgtagaatgc tttaatatcc tttggataat
    12241 cagttacaaa tacaggtttt ttgaatattt gctctgttaa atatctttca tgttcagttt
    12301 gaagatctat tccccactca acaggatatt cgaattcttt acctgattct tgaagtaatt
    12361 taacagcttc agtataagta acttttccga attctgagtt tacaacatgg tttaatctct
    12421 ctaataagcc tttgtcaaca aattggttga agaaagctat ttcctctgga cattcatcca
    12481 taacatattt tataacgtac tttaacatgt tttcagcaac ttccatgtca tctattaaat
    12541 cagcaaaagc catttcaggt tctatcatcc agaactcagc agcatgtttt actgtatttg
    12601 agttttcagc tctaaatgta ggaccaaatg tatagatatt tctaaatgct aaagcaaaac
    12661 attcagcgtt taattgtcct gaaactgtaa ggttagtttc ttttccgaag aagtcttgtg
    12721 agaagtcaat tccaccttct tctgatatag gaggagccat catgtctaaa gttgttactc
    12781 taaacatttc tccagcacct tcacagtctg aaccagttaa tattggagta tgagtgtaaa
    12841 caaatccttg atcttggaag aatttatgga tagcgtaagc tgcaacactt cttactctga
    12901 atgtagctga gaaagcatta cttcttggtc ttaagtgagc tattgttctt aaatactcaa
    12961 aagtatgtct tttcttttgt agaggataat ctgaatctga cattccctct actattattt
    13021 cttctgcttg aatttcaaat ggttgctttg aatctggagt ctcaactaaa gttcctataa
    13081 ctgatattga tgaacttata ggtaatttag aaatttcttt gtagtttgat atcttagcgc
    13141 tatcaaaaac tacttgaacg tttttaaaga atgaaccatc attaacttct ataaatccaa
    13201 aagcatttga tgctcttaaa gttcttatcc aacctgagat ttttactttc ttatcactat
    13261 attcactagt gtttctgtaa agacttttta ctaaaactac atctttcatt attatttcct
    13321 ctctttccta attaagtttt attccaaata aaaaagccct tcatccacat aattaatggg
    13381 acgaatggca ttattcgcgg taccacccaa attgctataa aatagccggc tttatgaaca
    13441 caaaaatgtt cttcaagtat aacggcctga aaccgtctaa gtctactctc ctaaaaggat
    13501 ttcggttaga aactcagaga tgttcttcaa aataggatac actgcagaac tcacaccata
    13561 atctgctcgc tgagagttac ttcctaagtt tactcttctc ttcatagtaa ataaaatagt
    13621 atttagttcg attaataatg attttacaat aaaacattaa catttgcaat attaacaaca
    13681 tagaaatata tagatatagt gtgtaatata ggagttttta tgtgcaattt ttttgaagaa
    13741 aagtttgaac aaataaataa attaaaacat ttaatgactt tatataaagg aaataaccaa
    13801 gctaaaatat ataaagaaca aatagaagat atttttaaag aatttcaaag ggattatata
    13861 agggaagaaa gtaatactat acttattttg acaaaggagg atttagctaa ttttaatggg
    13921 gaaaatggaa acccagccta tatttctata gatggtatta tatatgatat ttcaaatata
    13981 gaattgttta aacaaagtcc ttataacagt ttaaaacttg gaagtgatgt aacagagacc
    14041 ttttatgagt taaatgatgg agatgaatct atattaagag atattcctat ggtaggactt
    14101 ttagcagagc ctgaggaagc tgaatttatg gatagagtag aagagtatta tccacaacaa
    14161 aagcttaaaa taatgagttc agaagaactt aaaaaatata atggagagaa tggaaggtta
    14221 gcatatgtag ctattgaagg aatagtttat gatataacca atttatcagg tttggagcta
    14281 tttaacggga caaatttaag attaggctct gatattacta aagaatttaa aacttattac
    14341 agaggggata aggagctttt aaaggatgct aaagaagtag caatattaca tgatttcaat
    14401 gaaagtcaaa ggggaaaaca tattgaacac aatcttaagg agttaacctt aaatgaatta
    14461 tcaaaatata atggagaaaa agggaaacct ccctatatat ctatatttga cacaatatat
    14521 gatctaagct atgtagataa atggcaagaa aaaaacataa aagtaggata tgatttaaca
    14581 ggggaatata aagaggttta tgggaatgat aaatctcatt taaaaaatta aaaagttgca
    14641 ggggtcttaa tttgtggttt aaatgattat taaaaaaata aaagctagat taacttatga
    14701 actacttatt ataaaattta aagaaagaat tctataaaat ttttacacaa taaaattaaa
    14761 attctcatct aattgaaacc tagtagttta agttaaatct agctttttat ttattcttta
    14821 ccaaactcat aagagtatat taacttttga gggaagtttt ttaattccat atctttcata
    14881 gttcttctaa attcataagg aacagttttc atttctacct ttaatttaaa ttcatttgaa
    14941 aaatctaata taacataagt agcatgaggt ttaccgtttt taggtttacc aacactacca
    15001 tcatttataa taaccttatc acctattttc ttagcatatg gaatatgagt atgagcacaa
    15061 actaatacat cttccttaag ctcatccatt atctcctcta attctgaata atcttcactt
    15121 aaatattcat ttatttttct tggactacca tggacaaatt ttattgattt tccacacaca
    15181 ttcattgaaa tatcatcagg taagttatct aaataatatc tattagatgt tcttagttct
    15241 tctacagtcc aaggaagaga aaatgagttt atatctgtat ctctaatgta tgtataatca
    15301 ttatccacaa cagaagcatc ataatttcct tttaaacata aaatatttct tctttttata
    15361 agagctatta cttcattagg atgaggtcca taaccaacta agtctcctaa acatattata
    15421 gaatcaatat tttggttatc tatatcttct aaaacttctc ttaaggcata taagtttcca
    15481 tgtatatctg atattactgc tattctcaat ttaaagacct cctatttatt aaaaaatttt
    15541 actataaata ttatataaca atttagtgga caaaataaaa aaataaatat tacaaataaa
    15601 tgtatattat tagataattt gaaattatga tataatttat ttaatgaatt ttaactatta
    15661 gagaggagca atcaaggtga aagaaggaag agtaaaaaga attttagaag ctatgaaggc
    15721 aaatgatata cctcaaatgt tagtttcttc acctgcatca atattttatt taacaggtaa
    15781 gtggatatca cctggagaaa gaatgataac actttactta aatacaaagg gtgaacaaaa
    15841 gtttatagta aatgaattat tcccaataaa tgaggactta ggtgttgata ttttatggta
    15901 caacgatact atgaatccaa tagacatctt agataagata atagatcatg aagaagtatt
    15961 aggaatagat aaggattgga aggcacattt cttaattcaa ttattagata gaaattcagc
    16021 aaagaaattt gttaatggat ctcctatagt tgatagagtt agaatgagaa aagatgatga
    16081 agaaatagct ttaatgaaag aagcttcaag aataaatgat attgtagtag aaaaagcaat
    16141 aaagagttta aaagagggaa tgactgaaaa agaagtagta gaagttttag gaaaaggtta
    16201 tgctgaatat gggtgtcaag gatattcttt tgaacctata gttgcttttg cagctaatgc
    16261 agcagatccg catgcagagt caggagaaca aaaattagaa aaaggtatgg gagttctaat
    16321 agatactgga tgtagaaagg attattactg ttcagatatg actagatgtg tattctttgg
    16381 tgagccaaca gaacatcaaa aagaaatatt taacacagtt ttagaagcga ataaaaaagc
    16441 catagatatg attaaaccag gagttagatt ttgtgatata gataaagcgg ctagagatgt
    16501 tatagaaaat aaggggtatg gcaagtattt cactcataga acaggacatt caataggaat
    16561 agaaactcat gattttggtg atgttggatc aactaatact gatgaagtta aacaaggtat
    16621 gattttctca gtagaaccag gaatatatct tcaaggagat atgggagtaa gaatagaaga
    16681 tttagtatta gtaactgaag atggatgtga aagattaaat cacttaaaga aagaattagt
    16741 tgttattaaa taatagttta aatgaaaaga acattcttca attgaagaat gttcttttat
    16801 tttatgtaat ttaaattctt ctaactatta agcactaaat aagatacctg ctatagtagc
    16861 tgtcatacaa gtagctaata caccacctaa aagtgcttta aatcctaact tagctatagt
    16921 tggtttcttt tctggagcta atccaccaat accacctaat tgtatagcaa ctgaacttaa
    16981 gttagcaaat ccacaaagag cgtaagttaa tatcattata gttttaggtt ctaaagtacc
    17041 atttgctata tagtttgata attgtgagta agctaagaat tcattaactg ctaatttaat
    17101 tcctagtaag tctccagcta cgaaaacatc tttagttgga actcccatta taaatgctaa
    17161 tggagagaat aatctaccaa gaatccagtt taagcttaac caaggtgcac caaatattcc
    17221 acctattgct ccgaataaag cattaatcat agctataaga gcaacgaatg caagaagcat
    17281 agcaccaaca tttaaagcta atcctaaacc ttctgaagca ccatttgcag cagcttcaat
    17341 aacattttca ctttcaactt taaggtctaa agttccacca cctttagttt caggaacttc
    17401 agtttctgga actagtattt tagctaatat taatccggca ggagctgcca tgattgatgc
    17461 tgctaataag ttaccagcgt taacacccat agctacataa ccagccataa caccaccagc
    17521 aacagtagcc ataccaccta ccataattgc gcatatttct gatttagtca tgttctttat
    17581 gtatggttta actagtagag gagcttctgt ttggcctaag aagatattac caactgcact
    17641 gaaagtttca gcaccacttg ttcctaatgc tttagcaacc cctttagcaa taacttttac
    17701 aacaacttga actataccta aatgataaag tacactcatt aatgatgaga agaagataat
    17761 tgttggtaaa acttgtagag caaatatcat accgaatttg tctgtatcaa gtaatgggcc
    17821 aaataagaat gaactacctt ctttagtaaa atctaataat ttagttattc cattactaat
    17881 ccattcaaaa actgctctac cagcaggaac ttttagaatt agtaatgcga agataatttg
    17941 taataaaaga cctgttccaa ccaagcgcca gttgatcttc tttctatttt cagaaaaaag
    18001 aacagctata ccaataatac aaataagacc gattacacca ataaacctat ccacatttat
    18061 tctccttttc ttgtgttttt atactaaatt tatatttaat aaaagttaaa tttatataag
    18121 ttagtaactt tttattaaat ttcttttata atgttagtca ttaactctat gaatgttttt
    18181 ctaactttag ctgaagtttc cataacttct tcgtgattaa gcggttgctc taatatacca
    18241 gcagccatgt tagtcataca tgatacacct ataactttca ttccactatg attagctatt
    18301 ataacttcag gaactgttga cataccaact gcatctgctc ctaatatttt ggccattctt
    18361 acttctgcag gagtttcgta agttggacca ctaaacatag catatacacc ttcttttact
    18421 tctattccta aatttttagc tatatcttta acttgagatc ttaaatcttt atcataggca
    18481 tttgacatat cagggaatct agtaccgaat tcatttaagt ttttacctat taaaggattt
    18541 gatcctgata aatttaagtg gtctgatatt atcattaaat ctccaggagt atattcttta
    18601 ttaactgctc cagcagcgtt agtaactact aaagtctcaa ctcctaatag tctcattact
    18661 cttacaggac aagtaacttc ttgcattgag taaccttcat agtagtggaa tcttccttgc
    18721 attgcaacaa cttctttacc ttggaatttt cctataacta atcttccagc atgaccttca
    18781 actgtagata cagggaagtt tggaatttca ttgtatggga aatattcagc atcttctatt
    18841 tgatcagcta tagctcctaa tcctgaacct aatattaatc ctagtgttgg gttgtatttg
    18901 cttttacctt ttatgtactc agcagctgct ttaattttgt ttgataaatc cataatttta
    18961 cacctcttta gttaaatttt atttagtaat gattttaatt ttaaaatatt tccagtatgt
    19021 ggtaacgctt actttgaaat gtatagaatt ctaaattaaa cttaaaatta ttttattaac
    19081 ttttctgaac cttttaaaac aagttcagga gttattgtta tactatttaa ttctttttta
    19141 gagtcattat tcaaggattc tattaataaa tccataccaa tctctccaag ttctttagtt
    19201 ggcccattta tatgactaat atttatacct agtgtatcta atataggtat attatcgaag
    19261 gagattaatg aaatatcttt tgataattca agttttgctt ctcttaaagc ttttaaacaa
    19321 cctaaagtca taagattatt gcatacaaaa atggctgaag gtctattttt catagctaag
    19381 attttcttag tacattcata tccactttct tcttcataat ttccataaaa aattaagtca
    19441 ttatttatag gaatattatt cattaataat gctttttcat atcctaacaa tctatcctga
    19501 gctggtttag atgtcatacg cccagtaatt attgctatat ctttatgatt agcattaatt
    19561 aatgcttcta tggcgtcata agaaccttta atattgtcca caaaaacacc gttaaaattg
    19621 tgatatttaa gacttccatc aattaatatt actggtatac ctaaattagt aatagttttt
    19681 agatactcac tatttaaatc attttcaaca gaggttggac aaattataat gcctctaatt
    19741 ctttgttcct ttaatgtctt aatagctttt atttctctat ctatgctttc attagagtca
    19801 caaagaatca aatttagatt atgtacttct gcaacatcac ttattccttt tattattgat
    19861 ccaaagaatg gatttgtaat atcaggaact ataactccaa tagtattagt aatacttttt
    19921 gaaagacttc tagcaatagc gctaggggta taattatatt tatttataac ttttataaca
    19981 ttttcacgag tttccttttt tacatatcca gaattattta taactctaga aactgtagct
    20041 agagaaactc ccgcttcttt tgcaatatta tttattgtta cagacatttc attcaccatc
    20101 cttgtacatt attattatag attaactttg aaaataatta aacaaatatt ttccaaaaaa
    20161 tcaatctaaa gctatgtgtt atcgcttaca caataatgca caaaaaaaga attactatat
    20221 gtaaatgata acattatttt tataataatg caaacaaaaa aagactattt tttagtaaaa
    20281 tagtctttaa attaatttag tttaaaaaaa gttcaccaac tttaactaca tcaccagctc
    20341 caactgttaa aagtaagtca ccttttgata agttagcatt tacatattct aaagcttctt
    20401 catgagagtg tacatttgta catttaactc catgagctct tatagcatct cctaattgat
    20461 ctgaatttat ttcacctaaa tccttttctc tagctgcata tatatccatt aaaacaagct
    20521 catcacaatc atcaaaacat ttaacaaaat cattgaatag agtttttgtt cttgtgtaag
    20581 tatgtggttg gaatacacaa actattttat tgtgattcat ttttttagct gtgcttagag
    20641 tagcttttat ttcaacaggg tggtgagcgt aatcatcaat aacagttata tcatttttag
    20701 tacccttgta ttcgaatctt ttatgagctc ctccaaagga tgaaagacct gatactattg
    20761 catcagcgtt tatattgaat atttctgcta aacatacagc tgataatgag tttgatacat
    20821 tatgcatacc aggtacattt aaagaaagag aaagaagttt ttcttcattt ctaaatacat
    20881 caaaagtagc acaacctttt tcattaaact ctatatttct agcttggata tccccattat
    20941 taattccata agttaataca ttgcatttag ctacttcaag aacttccata acttttttat
    21001 catcagcatt tcctatgata tatccatcat taggtataag tttaacaaat tgtttaaatg
    21061 tatcttttat gtgatttatg tccttataaa aatctagatg gtctgcatct atgtttaata
    21121 ttattccaac ataagggaag aatcttaaga aactttgttt atattcacaa gcttctgtta
    21181 taaagtattc actgtttcca actctaaagt ttccatgaat aatatctaaa tctccaccaa
    21241 ctaatattgt agggtctaga tctgcactaa gggcaacatg agaaatcatt gaagtagtag
    21301 tagtttttcc gtgagttcca gctacagcaa cattgaattt atgacctttc ataatttgtc
    21361 ctaaaaattc agccctgtcc attaaaataa gattatcctc tttagcctta attatttcag
    21421 gattatcgct aggtattgct gcggtatata caactacatc aacatcttgt aaattatctt
    21481 tactgtgacc tatgtatatt tttgcacctt cttcttttaa tctattagta atttctgatt
    21541 cttttgaatc agatcctgaa actttgtaac cagcattaag taatacagca gctaagccac
    21601 tcatactaac tccaccaatt cctatgaaat gaactttttt attaatatct tttaataaat
    21661 cgaaagccaa gatatcaact cctttactta tatacactaa taattatata cattttttta
    21721 taaaagcaca caaaaaaatt ctttttaaaa tcattttatg aaaatttatt tggtttagtg
    21781 ctaaaaataa tcaacagata tttagatagt tattatagaa taacttaata attatcaata
    21841 aaaaagtatt aaaattcgaa atgagtgata cataaccggt taacagtatt aataaagttt
    21901 gttataatta ttttaaatgc agaataagaa gaaaaataat tataaaaata tatgtgtttt
    21961 gtaaagaata aaatatgaat tataagtgga aaatatatac tggaaattta tataggtcag
    22021 gtgattaata tggagaagtt aagtagaaat agtagagtat cgattataac aaaaattctt
    22081 atagagaatc ctaataaaat aataggatta aataagtttt cagatctttt aaatgcagct
    22141 aaatcaacta taagtgaaga tatagttatt gttagagaag tgttatctaa attttctatg
    22201 ggaaaagttg aaacaatagc tggtgctgct ggtggaataa gatatattcc agaagtggga
    22261 gaaatggaaa aaagaaagtt tttagaggaa ttatgtgata ttgcaagtga aagcaataga
    22321 gttgttccag gtaatttttt atatgttaca gatataatgt ttaatccaag tataataagc
    22381 aaagcttcta ttatattagc atcttatttt caaggtatgg atgtagacta tgtaataact
    22441 gtggaaacta agggagttcc gttagcttac gaagtagcta aatgcttagg agtacaatta
    22501 ataacagcaa gaagagatag taaagtaacg gaaggatcta cagtttcaat aaactatgtt
    22561 tcaggaacat caggtaaaat acaacaaatg tgtttatcta gaaaatcatt aaagcaagga
    22621 agcaaatgta tattcataga tgattttatg agaggtggag gaactgctaa aggtttagtt
    22681 gatttattaa aagaattcga ttctgagctt atgggaattg gagtattaat agacaataaa
    22741 ttatcaacaa acaaaataac aaaagattat gtatctttaa ttgatattgt tgagataaat
    22801 gaggatggag tgaaaatggt accatctatg taatttctat agttgttgtg ttaaagaata
    22861 atttacaaaa aatatgaaaa aaagaaggat ttctatattt ttcatagaat atacataata
    22921 acaacaactg gaggtgtacc ataatgaata ttacagatgt aagaattaga aaaatatctg
    22981 cagaaggtaa aatgaaagct atagtttcag taacttttga aaatcaattt gtagtacatg
    23041 atatcaaagt tatagagggt caaaatgggc ttttcatagc aatgcctagc agaaagacac
    23101 cagatggaga gtttaaggat atagctcatc ctatagatac gcaaactaga gagcaaattc
    23161 aaaaagctat tcttgatgaa tatgaaaagg ttaaaaactt agatatgcaa gaataatttc
    23221 aataaaagga agatgacttc cttttatttt tttgtattaa atgataattt tcatgcaaat
    23281 agcattaaat aagttattat tgaaataagt aatttattaa catataattg aaaatgagaa
    23341 atttgaagat ttctgaaaaa aataagagat taaatttaaa tagtatagct tatataataa
    23401 aatgagtatc acaaagaggt gtttttatga ataaatgtgc tataatatta gcagcaggtc
    23461 aaggaactag aataaaatct aagttaccaa aagttctaca caaagcttgt ggaaaagaaa
    23521 tggttaatca tgttattgat gctatgagaa atgcagagat agatgatgtt aatgtaataa
    23581 taggaaaagg tgctgaatta gttaaagaaa gaactacatc aaaaaatgtt agttattctt
    23641 tacaagcaga gcagttagga actggccatg ctgttaaatg tgcaaaagac tttttagaag
    23701 gaaaaacagg agtagtagct atatttacag gagatgctcc tttaataaag gctgaaactg
    23761 ttaagaattt agttgataca catatcaatg aaaagaattc agctacactg ttaacatcag
    23821 tgatagaaaa tccaactggc tatggaagaa tagttagaaa tggtgaatca gttgaaaaaa
    23881 ttgtagaaca taaagattgt aatgaacaag aaataaaaat acaagaagtc aatgcaggaa
    23941 tgtattgttt tgatatagaa agtttattaa cttctttaga gcaactttca aatgataatg
    24001 ctcaaggaga atattactta acagatgtta tagaaatatt aaagaaagag aacaagaaag
    24061 ttggagcaat gatcacagat tttgaagaaa ctttaggggt taattcaaga gctgagcttg
    24121 ctaaagttga aagtataatg agaaatagaa taaatagaac tcatttagat aatggagtta
    24181 ctataataga tcctctaaac acatatatag aaccagaagt ggttattggt aaggacacta
    24241 taatctatcc aggtaatgtt atagaaggaa aaacagtgat tggagaggat tgtattttat
    24301 atcctaattc aagaattaac aatagtacaa taggaaatgg cgttgaaatt caaagttcag
    24361 ttattttaga tagtaaaata ggtgatgaaa caactgtagg accatttgct tatgttagac
    24421 cagaaagtaa cattggagaa catgttagaa ttggtgattt tgttgaaatt aaaaaatcaa
    24481 caattggaaa taatacaaag gtatcacact taacttatat tggagatgct gaagttggag
    24541 aaagatgtaa ctttggatgc ggtactgttg tagttaacta tgatggaaag aaaaaacaca
    24601 aaacaatcat aggtgatgat tcatttatag gatgtaatac taatttagtt tcacctgtag
    24661 aagtaaaaga taatacttat atagctgcag gatcaacaat aactaaagag gttccagaag
    24721 gaagtttagc aatagcaaga gctaaacaac aaaacataga gggctgggtt gaaagaaaaa
    24781 agcttaaata agcattaaat atacaaatgc caaaggctag gtttaatttt aaggaggtct
    24841 tcactaaatg gaaaatcatt caaaaaacat aaagatattt acaggtaatt ctcatccaga
    24901 attagctaga gagattgcaa aggcgctaaa cattcctcta ggtaaagctg aagtttctac
    24961 tttcagtgat ggagaaatat cagtaaatat aaaagaaact gttagaggtt gcgatgtatt
    25021 tatagttcaa tcaacttgta gtcctgtaaa taataactta atggagttat taataatgat
    25081 agatgctttt aaaagagcat cagcaggaag aataaatgca gttatacctt actatggata
    25141 tgctagacaa gatagaaaag ctaagtcaag agatccaata acagctaagt tagtagctga
    25201 tctattaaca gcagctgggg cagatagggt tcttactatg gatttacatg cagcacaaat
    25261 tcaaggttat tttaatatac cagtagatca cttattaggc tcaccaattt tagcaaaata
    25321 ttttgttgaa aaaggattag ctgatagaga cgatgtagtt gttgtttcac cagatttagg
    25381 ttcagtaact agagctagaa agtttgcaga taaacttaat gctccaatag ctataattga
    25441 taaaagaaga ccaaaagcaa atgtatctga aataatgaat ataataggag atgttaagga
    25501 caaagtttgt atcttaatag acgatatgat agacactgca ggaacaataa ctaatgcagc
    25561 taatgctctt aaagatttag gagcaaaaaa tgtatatgct tgttgtactc atggagtatt
    25621 atcaggtcct gcatttgaaa gaataaataa cagtgctatt gaagagttag ttatgttaaa
    25681 tactatagct cttccagagg gagatggttt aaataaattt aaatcattat ctgttgcacc
    25741 aattatggca gatgcaataa atagaattta tgatgatgaa ccattaagtg gattattcca
    25801 agactaaaaa agagcgcata tgtcgctctt ttttttataa atcaataaaa atttaaactt
    25861 tttttaaaca aaaatcttta aatttgatta aaatgtaaaa ataaaataat tttgttaatt
    25921 ataaattgat aaaaatcaat aaatgtttta cactttattt atatacatta ataatatgtt
    25981 aaatgaaata attcatgttt ttaaagtata aaattgcaca aaataaggac aatgtagaat
    26041 atttacagaa aggattttaa gaggtgaatt ttatggaagg tttattagga aagattttaa
    26101 ttgtagacga tgatgagaat atatgtgaag tcataaaaat gtatttagaa actacaggat
    26161 ataatgttaa agtagctcat gatggaaagg ctgctaaaga agaatttgtt aactttagcc
    26221 ctaacctagt tgtattagat atgatgttac ctggaattga tggaatggaa gtattaaagt
    26281 ggataagaaa agatagtaac gttccagtaa taatgttaac tgctaaagga gaaacttttg
    26341 ataaggtatt agctttagaa ataggtgcag atgactatgt agttaaacct tttgagccaa
    26401 aagaattgtt agctagagta aaagctgtta tgagaagata ttcaggagaa gagcctgata
    26461 atgtagtatt aaacttccca ggtttaacta tagatgctaa ttcatataaa gttatataca
    26521 atggagaaga agttaagact ccacctaaag agtttgaact tttacactat cttgcaagca
    26581 acaagaataa agttttcaca agagatcaat tattatgtga agtttgggga tatgattatc
    26641 ctggtgattc aagaactgtt gatgttcaca taaagagatt aagagaaaaa ttaaacggtg
    26701 gagaagaatg gcaacttgaa actgtttggg gtgttgggta taaatttgag gtgaaataat
    26761 gagatatcgt ggcttagttc caaagttaat tttaacacta agcgtaacaa tatgcattac
    26821 tttctttatt gtaggcataa tactttctag cttgtttaga gaagaatatt atcaagaaag
    26881 attaaaaact ttatctgggg aagctgaagt tatagaacaa gttgtaaatt catttatgaa
    26941 tagtgaaact actttagttg aattagataa atctatatca tttataggag attctttaaa
    27001 agcagatatt tatttaattg atagttatgg ttacatatac tcagtttcta atgaaaaata
    27061 tgattattta aaacgcacaa aaataaatga tgatgatatg aaaatattaa aggaaaatac
    27121 tcctatagaa cagaagaaaa aaaatgaatt tatatattct aagcctattt tctgtaaagg
    27181 ggtatttaca ggagccgttt caatagttac tcctttaagt actttatcag attctcttgt
    27241 tagaattaat caaattgtat ggatgtgtgt agctatagct ataatttttt caggactatc
    27301 aatatatttt atttcaaaaa agataatcat aaaaccttta aaggttataa ataatgctgc
    27361 taaaaagatt gctaacggag aaattggaaa tagagttaat gtttcttatg atgatgaggt
    27421 tggtgaatta gctgaatcgt ttaatataat ggcaacatct attgaggaag ctgaattaaa
    27481 tagaagggaa tttatatcta atgtatctca tgagttaaga tcgccaatga catcgattaa
    27541 gggctttata actgctatat tagatggggt tattcctaag gataaagaag aatattatct
    27601 tgttatagta aatgatgaga taagtagatt aactagactt ataaatgact tactagattt
    27661 atctgctatg caagctggga aactagagtt taatataagt gagttagaat taaatagaat
    27721 catagagaca actgtattaa agatgaatca aaagtctgca agtaaaaaca taaaaattga
    27781 agttctttta gaaagcgaaa aattatatgt ttatggagat aatgatagat taatacaagt
    27841 tgttactaac ttagttgata atgctataaa atattgtgat gaaaatggtg aagttaaaat
    27901 atcaactaaa acaaagggaa gtaagattat tgttagcata tttaacacta gcaaaggaat
    27961 agacgaatat gatttaactc atatttggga cagattttat aaagttgaca aggcaagaag
    28021 taataaaact agtacagggt taggattgtc tattgttaga aatataatac tgcaactaga
    28081 agaagatatt tgggctgaaa atgtcaaaga taaaaatggt gagataaaag gtgttagatt
    28141 taattttact ttaactaaag ttaaataagt attgcatagg gggtctttat ggggaatttt
    28201 tttaagaact ctaaaaaggg gaagaatctc catggagtaa atgaaaaaaa ctatgacaat
    28261 atagtgatta aaaaggataa aaaaaggttt tggataaaaa gcttattaaa gttagttagt
    28321 tttgttattc tagttgtttt agtgagtttt attgccttta aacactatac taaagattta
    28381 aacatgaaag agcaaaaagc aaatctttta aatgaaataa aagatggtag atatgatata
    28441 actaatttag taaataaatc aggatgcagt ttagttacaa ttggtgatga tagtaataaa
    28501 ttagctatag gaaaagatga tggtaaaaat gtttctggat ctatcataag aacagatgga
    28561 tacataataa ctagttatag tgcaataaag gatttttcaa aaatttatgt taaaatatca
    28621 agttgtgata taagtccttt tgaggctaaa gttgttggat ttgataagga tactgacatt
    28681 gcagtattaa aaataggggc taatggatta aaaagtattt caacatcaga attagaagtt
    28741 ctagaagtag gtgaaaaagt tgcagcctta ggaaatacaa taagtgagga acctgtaggt
    28801 tttgtatcta atggaatagt tagttctcct ataaagaaaa taagcatagg agaagaaggt
    28861 cattctgatt ctaaggtttt tgatcttata gaaactaatt ctttaataaa ttcagataat
    28921 aatagtggta tattatgtga ctataatgga attgttattg gtataaatag tttatatttt
    28981 actaataaat tttctaaacc aggagtatac tatgctttag aaataaatga tgctttaaga
    29041 atagcagatt ctataataat aaaaggtggc gtaactgcaa gcgtaacctt aggagttaca
    29101 ggatctactt ttgcagatga aaagagcaat atttatggaa tttatgtaga agatgttgat
    29161 aaagatagta atgcttttaa ttcaggaata aaaccaactg atataatagt tgaagctgat
    29221 aataagaaga ttaaggatat agaaaactta gctgacttat tgaaaaaaca ttctgtaggt
    29281 gacataatta aacttaaagt tataagaggg gatatcacaa aggatatttc tgtaacttta
    29341 aattgattaa aagaatataa cccttcacat aatttagtga ggggtttttt gttgttttat
    29401 attaatagta aaagtataat taaagaatga tttaattaaa aattaagaat ttaatcaaca
    29461 tggaggttaa tatgatttta atagttggat taggaaatcc gggaaaacaa tatgagaaaa
    29521 ctagacataa cattggattt gatgtaattg attatatggc aaataagtat aatattgatg
    29581 taaataggga aaaatttaaa ggtatttgcg gtgaaggatt tatagaaaat aaaaaagtca
    29641 ttttattaaa acctttaaca tatatgaatt taagtggaga aagtataaga gaatttgcta
    29701 acttttataa gttagaagat gatgaaataa tagtagttta tgatgatata agtttagata
    29761 taggaagatt aagaataaga gaaaaaggca gtgctggtgg tcataatgga atcaagagca
    29821 taatacaaaa tcttggcgga gataaattcc caagagttaa agttggcgta ggacaaccta
    29881 aagataactt agtaaatcac gttttaggaa aattctcaaa ggaagatagg gaacatatag
    29941 aaaaggttat tccggtagta agtgatgcaa tagttgaaat tgtaaaaaat gatgctaagg
    30001 aatcaatgaa taaatttaat ggagttaata tagaatagtt tatttatttt agaatggtgg
    30061 tgtttagatt gaggttacag gggttaatgg ctcccttatt aaatagtgag gcttttcaaa
    30121 aggttaaatc ttttatggaa agcggaaatt atccagtaat gataaatggt ttatctgatt
    30181 caggaaaatc atattttata aatggtatat atgaggaatc agataagcct atagttgttg
    30241 ttacgcataa tgatattgaa gctagaaata tatacgagga tttatctttt tacttaccta
    30301 atgtgtatta tttaccttca agagagattg tgttttacaa tatagatgct atttctggtg
    30361 atttaagatg ggcaaggtta aaggttataa aggaaatgct aagaagcact aaaaaaatta
    30421 tagttacgtc tatagatgct tttgtagcaa cttatacacc taaggagctt tataaaagtc
    30481 atatattaaa aattaaagtt ggagatgaag tcgattttaa agagatttct cataaactta
    30541 ttgaatcagg ttatgaaagg ttagaaacag ttgaaggcaa aggtgaattt tcattaagag
    30601 gaggaatatt agatgtattt cctcctaatt ctgctaatcc ttttagaata gagttatttg
    30661 gagatgaagt tgattctatt agaaccttta atgtagaatc acaaagaagt atagaaaagg
    30721 taaagagagc agaaatattc cctgctaagg aagttatatt aagtaaagaa actatagaac
    30781 atgccataga aaagatgaga aaagaacttt ctgactttga aaataaagtt tctgataagg
    30841 aaataaaaga taaacttaga aaattaatag agaaaaatat agaatcatta caagaaaact
    30901 ggagctttga aactatagat agttatcttc cttttttctt tgataaacca gcaaccttat
    30961 ttgattattt aaataattat acctttataa ttgatgatgc taaaagatgt aagggaaagc
    31021 tagaaagtac tgtatttgag tttactgaaa actatgaagc cttcctagaa agaggaagca
    31081 tattaccttc tcaaattaat ttaagcttaa gtgctgaagt ggtagaagaa tcatttaaga
    31141 ggaacaaagt aataaatcta gttccttttg gagatggtgg atttatagaa tcatttaata
    31201 aagttgaagt aaaacaaagt actaaaagtg gatatcaagg tcaacttgat atgcttatag
    31261 atgatataaa agagaataag aaaaatggat ttagaacttt aatactttca ggaacaagac
    31321 caaggggaga aagattagta aatactttaa gagaattaga gatagagagt gtatataaag
    31381 atgaagttga tgaaataaat aatggagaag ttgtaataac ctttggagag cagaataaag
    31441 gatttgagta ccctgacata aaactttgtt taatatcaga taagagtgtt tttggtgagg
    31501 gtaaaagagt caaaaaagct aagagaaaaa aatcaaaagg tgtatctaaa attaagagtt
    31561 ttgctgagtt aaagccagga gactatgtag ttcatgtaaa tagtggtata ggtgtctata
    31621 aaggaataaa acaaatagaa gttcaaggac ataaaagaga ttatttagat attgaatatt
    31681 caaaaggtga taagttatat gtaccagttg agcaattaga cttagttcaa aaatacattg
    31741 gatcagatag tgcttctcct aagataagta aattaggagg aaatgagtgg caaaaagcta
    31801 aggctaaggt aagaaagtcc ataaatgaca tagctggtga cttagtaaaa ttatatgctg
    31861 agagatctac tgtaaaggga tataaattct caaaggatac tcaatggcaa aagcaatttg
    31921 aagatgaatt tccatatgaa gaaacaccgg accaattagc ggctattgaa gatataaaga
    31981 gcgatatgga atcaaacaag gttatggata gattactttg tggtgatgtt ggatatggaa
    32041 aaactgaggt tgctattaga gctgcattta aggctgtaat ggaagggaag caagtagcat
    32101 ttttagttcc tacaacaata ttagcagagc aacattataa gaatatgaaa agaagattcg
    32161 caggattccc tataactatt gatatgataa gtagatttag gactaagaag caacaaacag
    32221 aaactttaaa agctttaaaa gaaggaaatg ttgatatatt aataggaact catagacttg
    32281 tttctaaaga tattcaattc aaggatttag ggcttcttat tgtggatgaa gagcaaagat
    32341 ttggagtagc tcaaaaagaa aagattaaga gttttaagaa gaatgtggat gttcttactt
    32401 taagtgcaac gccaatacct agaactcttc atatgtcact tacaggggta agagatattt
    32461 ctgtaataga gactccacca gagaatagat atcctattca aacctatgtt gtagaacaaa
    32521 atgatcaact tattagagat gcaattttaa gagaaattaa tagaggtggt caagtttatt
    32581 ttgttcataa tagagttgaa agtattcaag aggtagctaa ttatatacag gagcttgttc
    32641 cagagtgtaa ggcaactgta attcatggac aaatgacaga gagacagtta gaaactgaaa
    32701 tgatagactt tatggaaaga aagtatgatg ttttagtatg tacttccata atagaaacag
    32761 gaattgatat ttcaaatgta aacacaatga tagtaaatga cgcagataaa atgggacttt
    32821 ctcagcttta tcaattaaga ggaagagttg gtagaagtaa caaggttgct tatgcatatt
    32881 tcctatatca aagagataag gttttaactg aggtggcaga gaagagactt aaggccttaa
    32941 aagattttac agagcttgga tcaggattta aaatagccat gagagacctt gaaataagag
    33001 gagctggaaa tatgatgggt tcagctcagc atgggcatat ggcggctata ggatatgatt
    33061 tatactgtag aatgttagaa gatactataa aaattgttaa gggtgaaata gaaagagaac
    33121 cagtagagac aactgtagat ttaaaagtgg atgcatttat tccttcaagt tatataaaag
    33181 atgaaattca aaagattgaa gtatataaga aaattgctgc tatagaaaat gaagaggatt
    33241 atgaatatgt tagagaggaa ttagaagata gattttctaa tataccaaat acagtttata
    33301 atcttatgga catagcttat ataaaaagca aagctaaact tttaaatatt gaagagataa
    33361 aagaaaagaa tgaagatata atatttatat ttgaaagtag agaaagaaca gataagagaa
    33421 tatttaaaat acttttagaa gaatataaag atgatatatt aattgaattt ggagataaac
    33481 caacaatttt atatagagtg aaggacaaga aaaaagaaga tgttttatct atttttaaag
    33541 agatgttaga taagttaata gctttaaata attaataatt tataaattga aatattactg
    33601 taacattgat atactaaaat agaatttaac tagaatatta tgtaaatttt aataggagaa
    33661 aggcgaggta ttagaatggt aagtgtaaaa aaaatagtcg catctgcact agttggagtt
    33721 ttaatgttta gtgccgttgg gtgtaatatg gtagaaaaaa ctcaagccgc tattgataag
    33781 actactgtag ctacagttaa cggagagaaa ataactttag gagaggttga ttccaattta
    33841 aaaggagtct ttactaaaat gaaaagccag tatggtgata aatatatgga tgaccctcaa
    33901 gttgcacaac aaatattaca gcaaagacaa agtgtagtac aaagcttagt aactgacaaa
    33961 gttttaggaa tcgaagctga taaattagga ataaaaccat cagaagaaga gattaagaaa
    34021 aaagtagatg aacaatttga gaatatcaaa aaaggtatgg gagataactt tgataaagct
    34081 ttagagggtg aaggatatac cgaagataca ttcaaagatg taataaaaaa tcaagttata
    34141 aatcaagctg ttcaagatta tgttattaaa gatgtaaagg taactgatga ggatgcacaa
    34201 aaatactatg atgagaataa acaacagttt gtagctaagg agtctggagt tcttacaaaa
    34261 cacttattat ttgaaaatga agaagaagct caaaaagctt atgatgaaat tcaatcagga
    34321 aaaacaacat tcaatgattt atttacaaaa tatgaaaata ataaatcaga gaataagaaa
    34381 cctatagcgg aaaatttagg agtagtacct gcagaaaact cagaattagt tcaagagttt
    34441 gtagatggat taaaaccatt aaaagaagga gaaatatcta agcctataaa aactcaattt
    34501 ggatatcata taattcaagc tggagctact tatgaaaaag gagcacagtt accatttgat
    34561 gaggttaaat cacaaataat acaaatttta aaacaacaaa aagattcaga gaaatttaaa
    34621 gctgatatgg atcaatggaa aaaagattta aatgttaaag tttatgatga taaactacaa
    34681 gaaggtttaa aaataagcaa atagttttta ttaagactta tggaagttat tccataagtc
    34741 ttttttttag agttttttaa gagatatttt cttaaattgg ctattaaatt taaagatgac
    34801 ataaatataa tcaatatgca taaaatttca aataaattaa atactattag tgtagacaaa
    34861 gattagtgat tagtatttat acagggaggt acatataaat gaaagctact ggaatagtaa
    34921 gacgtattga tgatttaggt agagttgtaa tacctaagga aataagaaga actttaagaa
    34981 taagagaagg ggatccttta gaaatcttta ctgatagaga tggtggagta atattaaaaa
    35041 aatactcacc aataggagaa ctaacagatt tttcaaagga atatgctgaa gctctacaac
    35101 aatctacagg acacataatt ctgatatcag ataatgatgc atttatatct gtaagtggaa
    35161 cttctaaaaa agagttttta gaaagaaaaa taagtgatga gttagaagat ataatggata
    35221 agagaaaatc agtagtatta agtgctacta ataaaaactt aataccaatc catggtgacg
    35281 atgatgaaag taaatatagt gggcaggtta tagcacctat attagctgag ggtgacgcta
    35341 tagggtctgt agttatactt tctaaagaag gtaaagcttt aggagaggtt gaacaaaaac
    35401 tagcagaaac agcagctgcg ttcttaggca aacaaatgga acaataaagc tatcataatt
    35461 aaatatttgt agtttaaata gtaataatac ctctgaattt aaaataatat aaaatgagac
    35521 ttaatttaga gaaagtaaat cattacttaa attaagtaat attaaatcaa ttcaatttta
    35581 ataagtatta aatatagtat tttaatataa tttggttaat ttaaatttgg aggtatttta
    35641 tgaagaaaca atcccttata aaaggtagtc ttgtacttgc ttgtgctggt attttagcca
    35701 agtttcttgg tttgtttttt agatggccat taataatgtt aataggagat gaaggtttag
    35761 gatattatca aatgacctat ccattatata tgctttttgt tgccattgca gcaggtatac
    35821 ctatagcagt atctaaatta gtatctgaaa aaaatgctct aggaaagtat gaggaaagtt
    35881 ttcaggtaat aaaagaagct ttaaaggtaa tggttttact aggaataggg acaagcattt
    35941 ttttagctgt aggtggtaaa tttcttctac catttttaaa atgggatgat aaggcgtatt
    36001 attctatact aggaatttgt ttagctccta cattaatagg aataataact cctattagag
    36061 gtttttttca aggatttcag aatatgactc caacagctat atctcaaata ctagagcaaa
    36121 taggaagagt tatagttggt gttggattag cttatatatt aatagataaa gggattgaat
    36181 actcagcagg gggagcatcc tttggagcag tagctggagc ctttgtagca gccttatatt
    36241 taattcctaa atatagaaag ataaaaaata gttttaaact aggtaagata aaaggtagtc
    36301 ataaaatatt tatggaaata ataaatatag cccttcctat atctataggt gctgctgtag
    36361 gaagtgttat gggggttata gattctgtct tagttcctca aaagctttta caagctgggt
    36421 ttacaggaca agaggctaca attttatatg ctcagcttac aggaaaagct actgtattta
    36481 ctaatattcc tatgacctta gccactgcat tagctgcctc aataatacct ataatctctg
    36541 aattatatat tcttaaagat aaagtacaag ttaaacaaaa aatagatact acaatgaaat
    36601 tatgcatgat agtttctatt ccatgttttt taggtttatt ttttatggca gaacctataa
    36661 tgaatctttt gtttccagga aaagctgatg gctttatgat attaaaatat ttatcaatca
    36721 gtgttccatt tttagttggg actcagataa caacatccat attacaagga atgggatatt
    36781 attatagacc tgtttttcat ctttttatag ggtgtatatt aaaagtctta ttaactatga
    36841 atttaatacc tataagatct ttaaacattt atggtgctgt aatagcaaca ataatatctt
    36901 attctgtggt attaatatta aatttactat atatggctaa gagatttaaa tatgctccaa
    36961 atataaaaga tgtaataata aagcctatct tcgcatcaat tttaatgatt ggtacagttt
    37021 tatatttata tacacaagct ttaaaatata gcgaaagtaa cagtatatct tgcttaatag
    37081 ctgtatttgt gggtattatt atatatatga tatcattgtt tgtacttaaa atttttagcg
    37141 tagaagaaat aaatggacgg ctaagaaatt aattttttat aagttgcgaa ggagaatatg
    37201 gaatgcttaa gataatgggt ctaggaccag gtgcctatga agcattaaca ataggtgctt
    37261 taaaagaatt aaaaaacaat aaaaatatat attttagaac agaaaaacat cctacagtgg
    37321 atttcttaaa agatgaagga attaaatttg aatcatatga tcatgcatat gaaaaatatg
    37381 atagctttga tgatgtgtat aaatatattg cagaggattt aataactaaa attaaggatg
    37441 atgaggattt aatatatgca gtaccaggac atcctttagt ggcagaaaaa tcagtaatta
    37501 atttaattga attatgtaaa gaaaataatg ttcagtatga agttttacca gcagttagct
    37561 ttgtagatgc tatgatggaa gctctgcaag tagatccaat agaaggtgta aaaataattg
    37621 atgcctttga catgaaaaat caaatattag ataagcgtat tggaactata ataacgcaag
    37681 tttataataa atttatagcc tcagaggtta aattaagact tttagaagga tatgaagatg
    37741 atacagaaat aatatttgta agagcggctg gagttgaagg tttagagagt ataagaaaaa
    37801 tacctttata tgaattagat tggcaagaag atatagatta tttaacttca atatatatac
    37861 ctaaggattt aggaaataaa aaagattttc aagatttatt agatattata gaaactttaa
    37921 ggaacccagg tggatgtcct tgggatagag agcaaactca tgagagttta aagagtgctc
    37981 ttttagaaga atgctatgaa gtgatagatg ctattgaaaa tgaggatgag gatgctttaa
    38041 tagaagagct tggtgatgtt ttacttcaag ttgtatttca tgcttctata ggtaaggaag
    38101 atggatattt tgatattatg gatgtaatag gtggaatatc aaataaaatg ataaatagac
    38161 atcctcatgt ttttgggaat gaagaagcaa atacttcaga gcaagtttta gtaaattggg
    38221 atgaaattaa gaaagaagaa aaaggtatta aaactttaac agaagaaatg caaaacatag
    38281 ctaaatctct tccagctaca acaagagctt ataaagttca aaagaaagct aaaaaggtag
    38341 gttttgactg gaatgatgta aattatgcta tggataaagt aaaagaagaa ttaaatgaaa
    38401 taaaagaggt ttataattgt gaagataagt caataataga aggtgaagta ggtgacttac
    38461 tttttgcttg tataaatgta gcaagattcc tagaagttga tggagaatta gctttagata
    38521 aaacaattaa aaagtttata aagagattct cttatattga aaatgaagca ataaaaaaca
    38581 ataagaattt aaaagatatg accttagaag agatggataa actatgggaa gaggctaaaa
    38641 caagtgaaaa ataagtgttt ttactaattt atggcttatt aaaatgaaaa aaagcataaa
    38701 tttaaaaaaa aatgcaaaaa tatgagtatt tttagggtaa aaattgaaag tattgactat
    38761 acaaagaaag aaatatatat tatgataata taagttgata ctagttcatt tggataactg
    38821 gtaaacactt attgcagtaa actaactgga attttaagga ggatgtaaaa gtgaataaag
    38881 cagaattaat aacaagcatg gctgaaaaaa gcaacttaac taaaaaagat gctgaattag
    38941 ctttaaaagc attcatagaa agcgttagcg aaactttaga aaatggtgac aaagttcaat
    39001 tggttggatt tggaactttc gaaactagag ttagagctga aagagaagga agaaacccaa
    39061 gaactaaaga agttataaaa atacctgctt caatagctcc tgtattcaag ccaggtaaag
    39121 agttaaaaga aagagttaac gcttaatttt agatatttta agtttggtta atttaacttt
    39181 aaaaaagccc gagttatttc tcgggctttt ttatctatta attaagtttc tttaaaatta
    39241 aggaggatat cctatgagat tagataaata tttaaaggtt tctagaataa ttaaaagaag
    39301 aacagttgct aaggaagctt gtgaaagtgg aagagtaata ataaatggaa aagttgcaaa
    39361 gccttcaaat gatataaaag aagaggatat aatagaaata ttatttggaa acagaacttt
    39421 aaaagcaaga gttataaaca ttgctgatca tgttagaaaa gaaaatgcta aagagatgta
    39481 tgaaatacta gaaggcgaag aagataaaga ataatatggt ttacttaata aaagtgacta
    39541 atataattta aaagattata ttagtcattt ttttgtgtaa agatgaataa tcataagtta
    39601 gggaaaatat ttttttacat aggaggcttt cttatgactg ataaaaaaag cgaaaataaa
    39661 accatacaag gtgctagcag tttatcttta gaaaatagaa aaattttatc tattacaggt
    39721 gtaaatgaag ttataaactt tgatgaagaa aaaataagtt taaaaacaga tttaggtcca
    39781 ttattaataa agggatctga gttaaagatg aataaattag atgttcaaaa tggggaggtt
    39841 ataataggag gatttataag taatattgat tatttaaata aaaaaaataa gcgcaaaaga
    39901 aatataaatt tactttctaa aatatttaaa taggtgggag atatgctttt agatataaat
    39961 attcaattta aaattgttat ttttgcaata ttatctggaa taataattgg atttttattt
    40021 gatatttata gagagttcag aggtgtttgc aaaaataaaa tattaataac aattgaagat
    40081 atattatttt ggatatggtg tagtttaatt gtatttgtat ttttactaaa atataattat
    40141 gcccttatag gtgtatatgt atatgtattt atgggaataa ctttagttat ttatttaaaa
    40201 acattatcta aatattttag aagaatacaa cataaaattc tagctatact attaaagaat
    40261 ctgagaataa tatccaaaaa tataagatat gcatttaaaa atacattcac aaattaaaaa
    40321 gttaattcat aagttaaaaa ttgcttgaat taagctataa tagtgtttac aatagactta
    40381 tatcgtaccg ttgcaagtat gggggtataa gagtgaaaaa aagaatgaat ttaaaaaata
    40441 tggtaataat tatattaatt gctatattgg ttatttctta tgttagacaa gaacttacaa
    40501 tgaacaaaat acacaaagac atagaagcca agcaaactca gcttaatgag cttcaagata
    40561 aaaataagaa gttacaagat gaggtaaacc aatcttcaac agacgaatac atagagagaa
    40621 tggctagaga aagattaggt atgattaagg atggcgagaa ggttattact aactcagatt
    40681 cacctcaaga caagcaaaat aattctgaag taaaaaacaa tcagaataaa taaggttatt
    40741 attaaaaata acatatattt tatttataaa tttaaggagg aaacttttaa acatgacctt
    40801 aatggcagga aacatattag agggtagaat aattaacatc acaaactttg gtgcgtttgt
    40861 tgaagtggaa ggaaagacag gtttagttca tatatctgaa gtagcagata cttatgttaa
    40921 agatataaga gagcatctta aagaagatga taaagttaaa gtgaaagtaa tatcagttga
    40981 tgataaaggc aaaataagct tatcaataaa gcaagctatg ccaccaaaga agaaatctgt
    41041 taaacctgca gattttgact ggaataatga aaagaaagct aagccaaaac aaaactttga
    41101 agatataatg tcaaagtttt taaaggatag tgaagaaaga cttcaagatg taaaaaaaca
    41161 tcaagatgta aaacaaagaa aaagaaaatc aatataatat ttcttaaaat cccttggagc
    41221 atatctaagg gattttaaat ttagttttta tattttatct ttgtagaatt tgatacttat
    41281 ttaaaataga atccaaataa taaaatatag attataataa aaatatttct ttgttttaat
    41341 tatttttaag cttaattttt atatgttttt tagttttttt ctaaaaaaat catatgagat
    41401 tatggagttt ttttatgagt ttttttaact aaaccttaga tttcatgagg gtttattaat
    41461 atttataata aattttataa aaaactttaa aaaatatgtt gacagtatgc ttatgcttat
    41521 gtataataaa tcttgtcgtt gggaaacacg atgacatgcc gaagtggcgg aactggcaga
    41581 cgcacaggac ttaa
//
LOCUS       DAEVGI010000008        40509 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEVGI010000008 DAEVGI010000000
VERSION     DAEVGI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 40509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40509
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.008"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            89..307
                     /locus_tag="K8M77_000428"
     CDS             89..307
                     /locus_tag="K8M77_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HBI7084956.1"
                     /translation="MKKIIVDRIEGHFIVCEDEKENILELKKDDVIGDVKEGDVLVKG
                     KEGKFCLDKVLTEKRKKEIEDLMKGMWE"
     gene            310..840
                     /gene="lepB"
                     /locus_tag="K8M77_000429"
     CDS             310..840
                     /gene="lepB"
                     /locus_tag="K8M77_000429"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI7084957.1"
                     /translation="MSSSNTKKKSFFLDWIIPILAALILAALIHKFLLFKIKVPTGSM
                     MPTVEIGDQLFVTKIYNPNNIKRGDIVVFNSDELKEPLLKRVIGLPGEHVEIKSDGSV
                     YINGNKLEEDYVKYQGGKTDMSFDVPSGKFLMLGDNRNNSDDARYWNNPYIDGKDIEA
                     KAQLTVYPFDRIGFVK"
     gene            865..3006
                     /gene="ftsH"
                     /locus_tag="K8M77_000430"
     CDS             865..3006
                     /gene="ftsH"
                     /locus_tag="K8M77_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBI7084958.1"
                     /translation="MFKDKKMLKYIVIYSIIAFGILLTFNMVKDEMLYEKVDYSTFMQ
                     MLDKKEVKSVNFSGNQIEITPSDSSNLKGKILYTTNPAVAGISQPDLIKDLTAAGVGF
                     NVTKPENYQLLGLLMSWVFPLILIFFVGRMMFSKMNNKMGGGVMSFGKNNAKLYAENE
                     TGITFKDVAGQDEAKESLVEIVDFLHDTRKYVEIGAKLPKGALLVGPPGTGKTLLAKA
                     VAGEAKVPFFSMSGSDFVEMFVGMGAARVRDLFKQAEEKAPCIVFIDEIDAIGKSRDG
                     AIQGNDEREQTLNQLLTEMDGFDSSKGVVILAATNRPEVLDKALLRPGRFDRRIIVDR
                     PDLIGREEILKVHSRDVKLSDDVSLEEIAKSTPGAVGADLANIVNEAALRAVKHGRKF
                     VIQEDLDEAVEVIIAGQEKRDRILSPKEKKIVAYHEVGHALVAALLNNTDPVHKITIV
                     PRTMGALGYTMQLPEEEKYLVSKEEMIDQISVMLGGRAAEEVVFNSITTGASNDIERA
                     TQSARNMITIYGMSERFDMMALEAMSNRYLDGRPVRNCSETTAAIADEEVLQVIKKAH
                     EKSIKILIENRELLDEITGVLLDKETIMGDEFMEIVYGKYPEKREADEKAKKEIQSLR
                     EQALAKRKEKEEAIKKAREEALRLEEEQRKQAELKAMIEAQEEAAKLSRANNEALDSS
                     KENEEVKSNVNDGATEEKKDDSSTNNKVDGE"
     gene            3227..5347
                     /locus_tag="K8M77_000431"
     CDS             3227..5347
                     /locus_tag="K8M77_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7084959.1"
                     /translation="MTDKKDIEFLVEEKKLEEVSNILNEEMLSYINKRKFVTQYILDY
                     RKNAVEEYKDDEDKLIEYFDHERYVKEQAFTTIDKKLKELTILKESPYFGRVTFNDLE
                     FDQKDTLYVGRFGVTPEGSYEPVIVDWRAPVASLFYHGSLGEASYTSPDGPIKCDIEG
                     RRQIIVKKGELKGVFDSAIDVKDDILQMVLSNNSSDKLKDVIMTIQQEQDEIIRKERT
                     SNIVVNGVAGSGKTTIALHRVAYLLYNYRKELEDKVLILGPNGIFMEYISQVLPSLGE
                     VGVKQETFASFALKEMDSELYIMSFDKYLEKILSEDKEFIEDAKYKNSYEIIKDLDNL
                     VKDMDKDYFHVEDVKYFGDLVISKEEIEEMFNKHYEYMPLFRRSEKIKRIILSKIKDK
                     RDEKVWELNEELRKEKEKLTPEELLIEENNLEFKRKLRIREIVKEVMDSRAKLDSWIS
                     REDVLDIYDRFNGNKKEYTINDLAPILYLAIKLEGKKATKDYRHVVIDEAQDYSPLQF
                     KVVRELTGTKYFTVVGDVNQRLIKYSDLAPMMELGKIFDDVNLEIYNLNKSYRSTYEI
                     MEYANKYLDEDRIIPIVRHGKPVEEIEFHNNEELSESIIESLKEFSNEGLESIAIITR
                     DKEELEKVYNLISNKVHLVKFDNEDVLYKGGNVIIPSYFAKGLEFDGVIIVDNGSSKD
                     ENEDLIKYIMSTRALHRLKEIQFK"
     gene            5805..6641
                     /locus_tag="K8M77_000432"
     CDS             5805..6641
                     /locus_tag="K8M77_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBI7084960.1"
                     /translation="MKNNIFKKMLSVAMIGGLTLSLAGCGAKTAKENGSNDKVAKIKE
                     SGKLVVGLSADYAPYEFHIMKDGKDQIVGLDIDIAKEVAKNLGVDLEIKEMEFGAIIQ
                     SVKNGMIDMGISGITPDEKRKEAVDFSDIYYEAEQGILINKDNKESIKGIGDLKGKKV
                     GAQMGSIQAEIAKGIEGADVKLLDNVNTLILELKTGKLDAVITELPVAKIASEVNSEL
                     AVADEVIKNSEGGSAIAIQKGNKDLVDEINSTIKELKENGKIDKFTNDAIELVPYQKK
                     EE"
     gene            6672..7343
                     /locus_tag="K8M77_000433"
     CDS             6672..7343
                     /locus_tag="K8M77_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI7084961.1"
                     /translation="MGGLDFSFLKEYFPVFLEGAKWTLIISAITVILGALLGTILCFM
                     KRFKFSIFKVKILRIISTAYVEVIRGTPLLLQIYIVYFGLPSFGIQLEVLPSCIIAIS
                     LNSAAYVSEIIRAGIDAVDKGQLEAARSLGMTEGMAMRLIILPQAIKNILPAIGNEFV
                     AIIKESSMASTIGVAELMYSSKIVTGATYKGFEPLIVIAMFYFVMTFSLGRVMSYVEK
                     RFSKS"
     gene            7340..8062
                     /locus_tag="K8M77_000434"
     CDS             7340..8062
                     /locus_tag="K8M77_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI7084962.1"
                     /translation="MINVRNLYKSFGKNEVLKDINETIKKGEVVVIIGPSGSGKSTFL
                     RCLNLLEEPTSGVINFEGEDITDKNVDINKIREKMGMVFQQFNLFPHKTVMENLTIGP
                     TKIKKISNGDAVKKGSELLEKVGLLDKKNVYPNSLSGGQKQRIAIARALAMEPDVMLF
                     DEPTSALDPEMVGEVLGVMKSLAKDGMTMVVVTHEMGFAKEVGDRILFMDEGRIIEEG
                     TPEDIFQNPKNSRTKDFLSKVL"
     gene            8280..10088
                     /locus_tag="K8M77_000435"
     CDS             8280..10088
                     /locus_tag="K8M77_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBI7084963.1"
                     /translation="MKEKVKSFFKCNWFFIFLVITLQIKSMLLLSMLRTPGSRGINFD
                     LMYFTPPAWWAHIAIVTLIASFVYLFKGKGRIWAGIVIDILVTILFVADIWYYRVNGT
                     FLSIRHIIEPGIFNPVGKSLFNLAKVDVVFIVDFIILFLVYNFTGLKNVKYKNNIKTR
                     LIAFICLFGISATVIGVSHYYIDIQRKSDKSFLRISWAPFQTISDSSPLGYHGYDIYY
                     YANRKETLTDAQKNEIKTWFDENKEDLPDNKYKGMLQGKNVIALQVESLENFVIGKKV
                     YGQEITPNINKLLKNSLYFDNIKEQNNSGTSSDCDIMVNTSILPVREGTTVFGYPWAE
                     YNTLQKILKSKGYSTVSTHPEVPGNWNWAEVHKAFKADEIWDAHQFDQSEIIGLGMSD
                     ESYLRQVGERLKSQKQPFYTFLVTLTSHGPFDMPKDKQYLNLPEDLNENMLGAYFQSV
                     RYTDEAIGEFINQLKEEGLLDNTVIMIYGDHGGVHKFYEDKIKDAPLEGDWWKDDEKE
                     IPFLIYNPSINGETISKEGGQIDFLPTIAYLLGFNRDTFDNTAMGRVLVNTNRNASIL
                     NNGEIVGNPTPEEKAHLEKSFNIADMIIQGNYFKNN"
     gene            10392..11096
                     /locus_tag="K8M77_000436"
     CDS             10392..11096
                     /locus_tag="K8M77_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI7084964.1"
                     /translation="MDKILLIIPAFNESENLPKLIASIKKESYDYDVLIINDYSTDNT
                     GKVAKELGVNVINLPCNLGIGGAVQTGYKYAYENEYDYAIQVDGDGQHDPIYLELLHK
                     KIKEGYNFVIGSRFIEKKGFQSTFIRRVGIKFFYHLIKFLGKEKITDATSGFRIADKS
                     VIKLFSQYYPSDYPEPETIMLLIKNNFKIAEIPVVMKERENGKSSINIIKSIYYMIKV
                     TLAIILSYFSKKRSYV"
     gene            11098..11448
                     /locus_tag="K8M77_000437"
     CDS             11098..11448
                     /locus_tag="K8M77_000437"
                     /inference="COORDINATES: protein motif:HMM:NF021575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HBI7084965.1"
                     /translation="MDRVLQFIILSVCILFTIYIIKMINKEKLELKYALTWLFSSLGL
                     IIIAVFPQILDWVSVILHILSPVNALFLIVIFFLLVIIFTLTVAISKSKKQIIAISQE
                     VGILKEKIERGENN"
     gene            11459..11791
                     /locus_tag="K8M77_000438"
     CDS             11459..11791
                     /locus_tag="K8M77_000438"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBI7084966.1"
                     /translation="MVLVNCIMLVSGQILWKIGLKDASMGSVKDIILLMFNKYVFSGL
                     VIYALATIYWFYIIKTNDLSKVYPMQSMSYIISLFMSYYILKESISINSILGTIVILL
                     GVFIIAMK"
     gene            11806..13074
                     /locus_tag="K8M77_000439"
     CDS             11806..13074
                     /locus_tag="K8M77_000439"
                     /inference="COORDINATES: protein motif:HMM:NF024629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HBI7084967.1"
                     /translation="MTFWEKDKRYRYTLWLCILAMFVIGTVCTLKYGNYFLLGDLDKL
                     NNDDVRYLHTAKVLAEQGKLVYHNMDPTLFIMPGYPIFIALIVKIFGSGSLGIIAIRI
                     SQLVLQCVCLYILYFLTKELVNKKTAIIACILTVLYLPEYVAANLILTEVLYKTLYML
                     LFYFSIIAIRKNKTKYYVFSGISWALVCLVRPNAAAFPLFIIIFWIVNKYSIKDMIKY
                     TSIVFVIFVTLFSPWWIRNYKLTNKFVLFTESSANPKLLGTFIRWGAPSFYKDIPKEY
                     KYDEFLNDEYLTEDEQNNLANYMIKRSFQEEPLKYTYWYTLGKTEELYKEAYYWKPIF
                     RVNDTRMNFTHISYITLGILGIIAMIRRKIKGGKMLIVFLLINTAVYLPFITFSRYGY
                     PNIFVFIIGAAYTLNVLFCKDEIQSEKSLI"
     gene            13089..14915
                     /locus_tag="K8M77_000440"
     CDS             13089..14915
                     /locus_tag="K8M77_000440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBI7084968.1"
                     /translation="MKKNEYRFLSILNNKFLLFFLFSLLIIIKEVFFTWIWSSSDSIA
                     KLQIFNMYMYWPKLLIHIVFAMTIASGIFLFNKNGRIIYILIADIVITILMFLDISYY
                     RNYGNFLSIRHLFHEELFNPLNKELFNFYKRDILLLIDFIILVPLSIFSLKNDSGKKS
                     RRSIKIFILSWIINGIIIYTSHSLVDIKGVTNGKLTLFEKSFAPQVNMDDLGMVGYHE
                     YDLTSYILKKDKKLSTEEKVEINKWFEENKETLPDNKYKGLGKGKNLIIIQWESLENF
                     AINYKVDGQEITPNMNKLLSNSLYFDNIYEQNKNGTTSDAELMANTSLLPISESAYFI
                     QYPWKKQNTLQRLLEKHGYNTATAIADKGGVWNWLENHKSFGVQTIWDSSYFNRDELI
                     GSNITDGSLFRQTEEKIKTLKRPYYLFMATATSHGPFDLPTNYRGLKLPKEIDDTKLG
                     GYLQSLRYTDKMLGEFLNKLKGDGVLDNSIIVIYGDHGGINKYYKKELENIDFANNNW
                     KQEYLKVPMLIYNPEIKGEVINTYGGLVDLLPTVGYIMGVDKSDFEKTAMGRVLVNTN
                     VNATINSSGQILGNPKDEKEIRHLQDMYKISNNIIESNYFNN"
     gene            complement(14972..16153)
                     /locus_tag="K8M77_000441"
     CDS             complement(14972..16153)
                     /locus_tag="K8M77_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBI7084969.1"
                     /translation="MFSNLIIKTFIKDYKNTENESVRSSYGYLASIVGIIVNIILFLI
                     KFSVGLISASIAITADAFNNLSDAGSSVITMIGFKLAKMPADKEHPFGHGRIEYISAL
                     IVAFMVMLVGFQFTKSSIERILNPTPVTFEVVPFILLIVSIFFKFWLSRFNKLTGNRI
                     NSSALKAASVDALGDVFTSTCVVISFLAAKFTTFPIDGYIGVIVSLAILYAGFTLVKE
                     TINPLLGEAPDPELAKNIREMVLSYDHIIGVHDLIIHNYGVGKCMASIHAEIPSDINV
                     MKIHDIIDMAEREISKKLKIYLVIHMDPICLEDDEIKQAKEEIDKIIKYNPLIESMHD
                     FRIVGHGNKKNLIFDIVVNPEKLRKVMSESELKEEIIKAVKETHPQYNCIITIDKDFT
                     C"
     gene            16519..17814
                     /locus_tag="K8M77_000442"
     CDS             16519..17814
                     /locus_tag="K8M77_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBI7084970.1"
                     /translation="MERVFANELINFLYESPTAFHAVKNVKDTLEFEGFKELKEADRW
                     NLEVEGKYFVTKNDSALIAFRVGKGDIAENGFKIIGAHTDSPGFRIKPNPEITVENTY
                     VKLNTEVYGGPILNTWFDRPLALAGRVSLVSKNPLKPVNKIVNINRPILIIPNLAIHM
                     NREVNEGYKINRQKDTLPLLSLVNETLEKGNYLVELLAKELYCEKEDILDFELYPYEY
                     EKGCLMGLNEEFISSGRLDDLSMVHAGIKALMDAEVSQATNVMVCFDNEEVGSATKQG
                     GDSDFLKTILERIVLALGKDREDFLRSLSESFMISADLAHAVHPNLGEKHDPVVRPVL
                     GKGPVIKIAASQSYTSDSDSSAVYEMICRNAGVNVQKFVNRSDMRGGSTIGPISSTHL
                     PIRSVDMGTPILAMHSIREFGAVKDHFDCIKSFTEFFRI"
     gene            complement(17907..18101)
                     /locus_tag="K8M77_000443"
     CDS             complement(17907..18101)
                     /locus_tag="K8M77_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7084971.1"
                     /translation="MSKKYPPKDRDFYSPEKHDLVSYSKTGKTYTKLPNPLEDDIVCN
                     NISYSEFDYIEGDMLDDDDI"
     gene            18492..19331
                     /locus_tag="K8M77_000444"
     CDS             18492..19331
                     /locus_tag="K8M77_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBI7084972.1"
                     /translation="MSYKLICIDMDGTLLNNKHEISERNKEAIKKATERGVKVAVTTG
                     RLFTSAKYYAGLLGVKTPIISCNGAFIREKDEDKVIYESVLNEDQLDRIYNVIKKYDI
                     NMAYFNTPNTVISEKIVPEDHGYKVMNRMMGESKEKVMFSEGIDFREAFKTHGKHILK
                     AICIENDSNKLNDLFKAKEELKKYEDLEVVSSSPSNFEVMNKGTSKGRAVKVLADILN
                     INREEVMCLGDSENDLSMIEFAGLGVAMGNAEEFLKEKADYITDTNENDGVAKAIEKF
                     VLD"
     gene            complement(19539..22868)
                     /locus_tag="K8M77_000445"
     CDS             complement(19539..22868)
                     /locus_tag="K8M77_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HBI7084973.1"
                     /translation="MKDNILKKIKLNKPLSFFILIIWCAVVAFLFLTAPSLSKLVSEK
                     GGIQLPDDYSSKISDILQSDNKFNSKDSYIAVFHSSNKLTDENLKNIKDTIDRLKSKE
                     NSDYIQSINDSFDTPQLKNQLNSENGKTIIALMTVNPKDSSVSTIEKTLNDELKTPGV
                     ETYVTGQSLIENDVNVAAQKGLNKTQTITVIFIFIVLILLFKSVVTPFIPLLTVGISY
                     LGAQSVVGILADKFNFPLSNYTQIFMICIMFGIGTDYSILLLNRFKEELANGNNKLDS
                     VKTTFKTAGKTVLSSATPVFVVFASLNFIGFNLYRSAVAVAIGILFLIAALFTIFPAV
                     MIILGKNIFWPVKGTFKAEENRLWAKLANKAFSKPIPILGAIFTILLLPILFNNFHQS
                     FNNLNEIGDTYSAKEGFNIIADDFGIGQASPVSIYIENDDDMRKPEYVALIEKLSNNL
                     QNSSNDIKSVLSVTRPTGVRLDEIYVNDQAGQVSQGIDKANNGLNKINDGLSDASKKI
                     DSSKSKLKGAEEGTAKLQEGTKKTETGVSKLQSALNKLVESIREEHNGATELKDSIDG
                     ALDKVNSLKLSKSQLEEDYSAIVNSANDVLENLNKISSLTGNPNSVNVDTSKLKESLS
                     SLNSHLSEYSKSHPEVLNDENFKKFSDDINNLNNNSNNLQGTFNNTPEIKAINESIGA
                     LKVLINTLNSKKDTVINDLDKFNSGISEIENGLKALDSGLGKSLAGGEEISSKVPEIS
                     NALNQIANGQSKIQDGFKEFSGQINELSNGLEAGSNGLTEIQNGLKYANGFINDWSNL
                     SYNLSGICVPIEVFSNDEFKKSLNQYMSSDGKLAVINVITTKNPYSNEAVNEIPNLKS
                     LVQDGVKGTKLENANIGIGGMASTTYETKRMADSDYDKALIFVIVGVFITLIIILRSL
                     TMPIYLMISLLLTYFTSISVIQFVVTKIMGYPGISWITSFFGFVVLMALGIDYSIFIM
                     TRFNQDLNGSIKERIIRTMKSMGGVIISAVIILSGTFAALLPSGVLSLIEISMVVLTG
                     ILLYVVFVLPLFIPAMVKIFGKANWWPFKPALSKEDKSLDDSTTIVTTVVTTTTTTTT
                     TTKKGSSKVTKKESKTTTTKE"
     gene            complement(23129..24724)
                     /locus_tag="K8M77_000446"
     CDS             complement(23129..24724)
                     /locus_tag="K8M77_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBI7084974.1"
                     /translation="MSDLIKEIEKRRTFAIISHPDAGKTTLTEKFLLYGGAIRMAGSV
                     KARKAATHAVSDWMEIEKQRGISVTSSVMQFNYGGYCINILDTPGHQDFSEDTYRTLM
                     AADSAVMVIDGAKGVEDQTRKLFQVASLREIPIFTFVNKMDRETRDPFQLLEDIESEL
                     GIKSYPMNWPIGCGSHFKGVYDRATNTIHLFDGGNHGQSEVSTITGDLEDPKFKELLG
                     EDLHNKLMEDVELLDIAGDEFDLEKVRKGELTPVFFGSALTNFGVEPFLNDFLKLTTS
                     PLPRNSSIGEIDPMTEPFSAFVFKIQANMNKAHRDRLAFMRICSGKFEKGKDVFHVQG
                     GKKVKLAQPQQFLAQDREVVEEAYAGDIIGVFDPGIFSIGDTLCLNPKKFKFEGIPTF
                     APEHFARVKPVDTMKRKQFIKGVTQIAQEGAIQVFKELHIGMEEIIVGVVGVLQFEVL
                     EYRLKNEYNVDIKMERLPFRYVRWIENDNIDVDSLNLTSDTKKVKDFKDRNLLIFQND
                     WGISWAIDHNPGIILSGVGKSND"
     gene            complement(24939..25544)
                     /locus_tag="K8M77_000447"
     CDS             complement(24939..25544)
                     /locus_tag="K8M77_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI7084975.1"
                     /translation="MPVRIAILGGPRCGKTTLIQQLYVEFKIRGLDVGAATEYSTEYL
                     KEKGMIETISEQYGIYLGQLRLEESLNQFDYALTDYATFVPYIYGRFMLGDKKRSVKE
                     IEILKDLYHLALKDLQKYDHIFFVPREFGYTQDGIRWQDESTASAVDNSLKQFLDSEN
                     VKYTTIEGSTKDRAKQILSIVGLDNKDALPTMRELKKKNSK"
     gene            25930..26730
                     /gene="yidA"
                     /locus_tag="K8M77_000448"
     CDS             25930..26730
                     /gene="yidA"
                     /locus_tag="K8M77_000448"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBI7084976.1"
                     /translation="MYKLVAIDMDGTLLKEDKTISEKTKEAIKKAREKNVKVVLATGR
                     PVDGVKRYLEELDLFHNDEYVLTFNGAIVKEIGPDRVICRDTLKGSDLKYLYEVSKNA
                     GVNIHAFSNFGCVTPKMNKYTELEGRINGIEVCEADYNEIKEDEEIIKIMMIDEPEVL
                     EEGIKKLPEEVYEKYTVVRSAPYFLEFLSKTCNKGEGVKSLAESLGIKREEVIAIGDA
                     GNDLHMIEYAGLGVAMGNAFEEVKEKADFITKSNEEDGVAFVFEKFVL"
     gene            26767..27429
                     /locus_tag="K8M77_000449"
     CDS             26767..27429
                     /locus_tag="K8M77_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBI7084977.1"
                     /translation="MESINDIPIKSECSNEKSYLFYNVAKRCFDLICSLIALILFSPI
                     FLILYIWVKVDSKGPAFFGHKRIGKDRKYIDVYKFRSMVSNAKEVFDNFTPEQKAEFE
                     KNFKLDDDPRITKCGAFLRKTSLDELPQLINIIKGDMSIVGPRPIVDKEVEKYGEYAD
                     KLFSVTPGLTGYWQANGRSDTTYEERVQMDMYYIDNRSLWLDIKIIFKTAISVIKKEG
                     AV"
     gene            27430..28344
                     /locus_tag="K8M77_000450"
     CDS             27430..28344
                     /locus_tag="K8M77_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI7084978.1"
                     /translation="MKISIICPLYNAENYIMSLHNKILEQEKPEGTEVEVSYVLTKCK
                     DKTEEMLKNMNLHYTLIEPKDFSHSKTREMMAFKSTGDIIVFISQDVMMKNNFWLKNL
                     VQPIIDGECEASFSRQLCTNNTIEKYIREKNYPEESRVVSKKDINKYGLLTFFYSDAS
                     SAVKTSVYKELNGYDNKRLLINEDMYFAEKLIQAGYRIKYCSDSEVYHSHVFTLKSLY
                     ERYFDTGVFFRQNSQFLNYKGNKSGKDLAIHVLKRSLEDKNFKVTLNILPNFGARFIG
                     SFLGKKYESLPRSFVMASSMSKKVYSQI"
     gene            28367..29248
                     /gene="rfbA"
                     /locus_tag="K8M77_000451"
     CDS             28367..29248
                     /gene="rfbA"
                     /locus_tag="K8M77_000451"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI7084979.1"
                     /translation="MKGIILAGGSGTRLYPVTKAMSKQMVPIYDKPMIYYPMSVLMLA
                     GIREVLIISTERDLPNFKELFKDGSELGLKIEYKVQEAPNGLAEAFIIGEEFIGDDNV
                     AMILGDNIFYGQNFSSNLKEAADLEKGAMVFGYYVQDPKAFGVVEFDENGKVISLEEK
                     PEHPKSKYAVPGLYFYDNTVIEKAKGLKPSARGELEITDLNRLYMEEGTLKVNLFGRG
                     MAWLDTGTHASMLQASNFVEAVQNTQGTYIACLEEIAYRKGWISSEKVLNLAKPLMKT
                     GYGKYLVDIVEELEQKK"
     gene            29354..29923
                     /gene="rfbC"
                     /locus_tag="K8M77_000452"
     CDS             29354..29923
                     /gene="rfbC"
                     /locus_tag="K8M77_000452"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBI7084980.1"
                     /translation="MSNFKFIETKIKDLYIIEPKVFGDERGYFMETYSEKAFEEAGLT
                     MKFVQDNESKSKKGVLRGLHFQTKHTQGKLVRVTQGEVWDVAVDLRKGSPTFGQWEGV
                     HLSAENKRQFYVPEGFAHGFVVLSDEAVFNYKCTDFYAPEYDSGLLWNDKDVNVEWPL
                     EGIEEIILSEKDKKQKTLKELDIPFVYEG"
     gene            29937..30824
                     /gene="rfbD"
                     /locus_tag="K8M77_000453"
     CDS             29937..30824
                     /gene="rfbD"
                     /locus_tag="K8M77_000453"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBI7084981.1"
                     /translation="MKILITGSNGQLGNELQSIIKSGKAEIGSVSENIKNSEVRALDV
                     DKLDITSLENVKNVLNEEKPDVVINCAAATNVDGCESNEDFAFKVNSLGARNLAMVCE
                     EIGAKLVQVSTDYVFSGVGEKPLTEFDLTAPYSVYGKTKLLGENYVKEFCSKYYIVRT
                     AWLYGYVGHNFVYTMMRLGKEKESLNVVNDQKGNPTHANDLAYHILKLIETEEYGIYH
                     CTGKGECTWYDFAKEIMELAGNKCIVNPCTSEEYKTPAKRPEYSSLDNMMLRCTIGDE
                     MRDWKEALKSFFENQQKNQ"
     gene            30852..31901
                     /gene="rfbB"
                     /locus_tag="K8M77_000454"
     CDS             30852..31901
                     /gene="rfbB"
                     /locus_tag="K8M77_000454"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI7084982.1"
                     /translation="MKTYLVTGGAGFIGSNFVLYMLNKYEDIKIINLDKLTYAGNLEN
                     LKSIENDERHIFVQGDICDKELVSSLFEKYEIDYVAHFAAESHVDRSIREPEVFAKTN
                     VLGTVNLLNCAKNAWETEEGWKEGVKFLHVSTDEVYGSLGETGYFMETTPLDPHSPYS
                     SSKASSDLMVKAYADTYKMPVNITRCSNNYGPFQFPEKLIPLLINNCLNHKDLPVYGD
                     GMNIRDWLFVEDHAKAIDMVINGGRLGEVYNVGGHNERTNIQIVKTVIEYLHDNVDEE
                     ITENLIKYVEDRKGHDRRYGIDPTKIKEELGWYPETTFEVGIKKTIKWYLDNKEWMEN
                     VTSGDYQKYYEKMYR"
     gene            31926..33140
                     /locus_tag="K8M77_000455"
     CDS             31926..33140
                     /locus_tag="K8M77_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBI7084983.1"
                     /translation="MNLRNKSNTKYELFIYFVLFSIFIRDFKIYIPLFVLNILYVIYL
                     IFKKEIHINLKKWNITLALFIIWTVINTIIASFIFKYPVYYSNVIKLLLNMSFLLSTY
                     FVVDGANMKFHKKTLVRFLEFIILINFIQIIYIYFDGKLFNDFFSGALTESSDAAYTI
                     SSYHNIIGAENKNIWATKFALIYMIYLYICAFDNFNINLIEKISFIILGIVTVVLLLS
                     RTAQVAIIIPIVFLAFYSMRNLNYKYRIGIYAVSGILFLGVLFIFFDKFFHLKFNMTD
                     GGFTRLYIWKEFIISVFDTHFMVGNGIGYSAYFIQDILGRFESNLHNVFLNIFFELGI
                     IGIVLYITFIIQFFKDFIIRKNILKNLFMIIFPMLAIVCLQYLGYDNDLVVTLTLLII
                     INMIMKKDEQRI"
     gene            33137..33985
                     /locus_tag="K8M77_000456"
     CDS             33137..33985
                     /locus_tag="K8M77_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI7084984.1"
                     /translation="MIRKFSLIMATYGRKDEVGYFLESLMNSNYPMDKVEVIIVDQNK
                     EIDLEPIISKYKEKIKVVHIKSNERGLALNRNKGLEIATGDIIAFPDDDCEYLVDTLN
                     IVNNYFENEHCDLLMGRIVERNGEDSLRDWPKEKIEINKNNFYTKCSSVTMFLDSKKC
                     FVKFNDQLGAGKYFGACEDADLIYKNCKRGLKIIYNPELQIYHPHYDSNSNMDDKKIY
                     SYGLGFGAMVKSNFDFSMLILFIKAEGYHFLKSLIYLITFKTTKSKRSWLAFKSRIKG
                     FIEYKN"
     gene            34079..34819
                     /locus_tag="K8M77_000457"
     CDS             34079..34819
                     /locus_tag="K8M77_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HBI7084985.1"
                     /translation="MSKVKRKLKNIVRNIMLSETFANTNLVKNMRKNHKEKLEEGRRV
                     LFKEHAKDCLETIKETLDKNNLHFWLDYGTLLGAMREKDFIAHDLDIDLGMFYENQVE
                     EVEKAFKEANIKKVREFTLDGKVVEQTYSYKGLYFDIFYYFKDENLMWTYGFTFKNNK
                     LNKVSYKDKDISTGFEGMRYFVKKRGLEKIMFKGKEYTVPENPDGYLRENYGPNYMTP
                     IKEWDYVEAPTNQEKLNGGDIVMIEYYN"
     gene            34828..36279
                     /locus_tag="K8M77_000458"
     CDS             34828..36279
                     /locus_tag="K8M77_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HBI7084986.1"
                     /translation="MNKSISKNIIFKFLLNIFNVVVPIIIGPYVLRVLGPDLMGTINF
                     SQTIYGYFFIFAGFGVYQYGLREISRVRDDKKKLSQVYTSLFTLTFITNILTTIGYVL
                     FVQFNYSGTEIYVACMILTFNLLANIFYTEWVNEGLENYDFITIKTIIIRIVYVVFLF
                     ILVRSANDLKEYMILLVLSTFLNNIVSFIYIRKRIPFDFSDLKLLRHIKPMFLVVILS
                     NANVLYTQLDRYFIGEYINMDSVAYYTTAQNISNIINTLLLTVIYATIPRMSNYIANE
                     NHDEYKGLLDKISRMYFIVLFPAAIGMLVLAKEVILIYGGSKYLDAIPMLKVFAIYII
                     TLGFETILSNQVMYIRGKEKEQVKITFIGGAVNLVLNVTLLWTGYFNGTNAVITTMLA
                     NIVVIILEYLYIKFVMDLDFNIFSLDKMKYLAISLLFIPITYFIGKFISGVILFTLAV
                     MTINSLVYFLILLVIKDDCLLEIINKFTKKLRR"
     gene            36300..38723
                     /locus_tag="K8M77_000459"
     CDS             36300..38723
                     /locus_tag="K8M77_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBI7084987.1"
                     /translation="MIKKFIIATVITLSVTSINVIALENVNNNSDENKYSTLVGETYE
                     IVKKPNMFFLIPNDNVESYKDENGIKREEFKKDKEGQESINRLVTKTKSKYEIALAHE
                     NGKYTFLDSANTKEEAENKVKNLSGKYNTFAAMPVVLNDSGQVAYSEKSMGRLVKYKN
                     GSPAGYGEITNIYANPNLINDFTYINHGYVDDVPIIEDRGNVAKIEVGGYEGWVNKDT
                     SSGNYDLVIVPLNQVKNPSYYIVRDGELIHYISSDLTNYSEGGYEVVIGPAPNFLNEN
                     VKYYSYDNKYFYKDLSTLIGDLQNDNHNNSVNANNPFYPYYMYLPFRSKTTFTAEELN
                     NFIDKKTKSYSKLRGTGQAFIDAQDKYGANALLLLGLAANESAWGTSQIAQQKNNLFG
                     INAIDSSPGASANSFETVEGCINDFAKYYISRGYSDPEDWRYFGGYLGNKGSGANVKY
                     ASDPFWGEKAGQNAYIADYWTSGKGIAGLKDYNYYQLGIYTGASSVTNKDNEKLYDVG
                     SLYTERVEKIGATTILTSKEKINHNGKECYEINPVRTTPVISNGSPVAFPGPYDWNDK
                     GFVDASKVKLINEGKYSENVMGKWELNNGIWYYYIDGKYVTGWNNIDNNKYYFDQSGK
                     MQTGWLCIDNIWYHFRDNGTIDIGWREIDGYWYYFNNDGEMQKGWQTIGKYKYYFNDN
                     GVMNIGWRKIDNIWYYFNINGEMQTGWLFENNIWYHFETTGSMTVGWKKINNDWYYFN
                     SNGEMQTGWQNIGTPKYHFRDNGVMDIGWREIDGQWHYFNENGEMQTGWQYINGNSYY
                     FDEKGIWKQ"
     gene            38852..40504
                     /locus_tag="K8M77_000460"
     CDS             38852..40504
                     /locus_tag="K8M77_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBI7084988.1"
                     /translation="MKRKSIALVLSLGLLGSVTLGSIETVYAEGNKNTTNISEVKVDN
                     KVELSDKKNKEIKNGWEIINDKWYYHESDGSLATGFKKINDEWYRFDNNGEMQVGWQY
                     INDIWYHFRDNGPMDTGWRKINNDWYYFNNDGEMQTGWQSIGNKRYHFRDNGVMDTGW
                     RSLGSDWYLLNSDGEMQTGWQHINNIWYHLEDDGKMNTGWQDINGDKYYFNNSGEMQT
                     GWKYINDIWYHLRENGPADTGWRKINNDWYYFDNIGKMQTSWQDIDNSTYYFNDNGVM
                     SIGWRTIESEWYYFNNSGEKESNKVIDDWYLDKNGVGNKIVTEGVYGKSGKGRDLEYY
                     RIGHGKKVLFSVFGVHGYEDAWSGDSQELKIIAEKSVNRLRSEYNTKGIDLSEWSVYI
                     IPSANPDGRIDGWTNNGPGRTTVTTKNDINRSFPTGFSPFYSARNYTGPTALGSPEAK
                     ALYSFINKTMEGATEKVLLDVHGWENKTIGNPSIARYFDNEFGFRNINKYPGGFVITY
                     GNAIGAKSVLVEFPVPYSHQDILRRDFSGKFANGLVNILLNN"
BASE COUNT        15862 a         4216 c         6869 g        13562 t
ORIGIN      
        1 aaggtaaaaa attctaaaat tattgaggaa taattttaaa ttgattttaa agatttctca
       61 tactaatatt cggagcaagg agtataaaat gaaaaaaata atagtagata gaatagaagg
      121 acattttatt gtatgtgaag atgaaaaaga aaatatttta gaattaaaaa aagatgacgt
      181 aataggtgac gtaaaagagg gggatgtttt agttaaaggc aaagagggta agttttgctt
      241 ggataaagtg cttacagaaa aaagaaaaaa agaaatagaa gatttgatga aaggtatgtg
      301 ggaatagtaa tgagttctag taatacaaag aagaaatcat tctttttaga ttggattata
      361 ccaattttag cagctttaat attagcagct ttgattcata aatttttatt gtttaagata
      421 aaagttccta caggatcaat gatgcctaca gtagagattg gtgatcaatt atttgttact
      481 aaaatatata atcctaataa tatcaaaaga ggagatatag tagtttttaa ttctgatgaa
      541 ttaaaagaac cgcttttaaa acgtgttatt ggattaccag gtgagcatgt tgaaataaaa
      601 agtgatggat cagtttatat aaatggaaat aaacttgaag aagactatgt aaaatatcaa
      661 ggtggaaaaa ctgatatgtc ttttgatgtt ccaagtggaa agtttttaat gttaggtgac
      721 aatagaaata atagtgatga tgctagatat tggaataatc cttatataga tggaaaagac
      781 atagaagcaa aggcacagtt aacagtatat ccttttgata gaataggatt tgtaaaataa
      841 acgttagaga aggaggaatg aggtatgttt aaagataaaa aaatgcttaa atatattgtc
      901 atatatagta ttatagcgtt tggtatatta ttaaccttta atatggttaa agatgaaatg
      961 ctatacgaaa aagtagatta tagtactttc atgcaaatgt tagataaaaa ggaagttaaa
     1021 tcagtaaatt tttcaggaaa tcaaattgag ataacacctt cagatagtag taatttaaaa
     1081 ggaaaaatct tatatacaac taatccagca gttgcaggta ttagtcaacc agatttaatt
     1141 aaagatttaa ctgcagctgg ggtaggattc aatgtaacta aaccagaaaa ctatcaactt
     1201 ttaggtttat taatgtcatg ggttttccca ttaatactta tattctttgt tggtagaatg
     1261 atgttctcta aaatgaataa taaaatgggt ggcggagtaa tgtcatttgg taaaaataat
     1321 gctaagcttt atgctgaaaa tgagactgga ataacattta aagatgttgc aggtcaagat
     1381 gaagctaaag aatccttagt agaaattgta gactttttac atgatacaag aaaatacgta
     1441 gaaataggag ctaagcttcc aaagggtgct ttattagtag ggcctccagg aacaggtaaa
     1501 actttacttg ctaaggctgt tgcaggagaa gcaaaagtac catttttctc aatgtcaggt
     1561 tcagacttcg ttgagatgtt tgtaggtatg ggtgctgcga gagtaagaga tttatttaag
     1621 caagcagaag aaaaagctcc atgtatagtt ttcatagatg agattgatgc tattggtaag
     1681 agtagagatg gtgctattca aggaaatgat gaaagagaac aaactcttaa ccaattatta
     1741 actgagatgg atggttttga ttcttcaaaa ggggttgtta ttttagcagc tacaaataga
     1801 ccggaggttt tagataaagc tttattaaga ccaggtagat ttgatagaag aataattgtt
     1861 gatagaccag atttaatagg tagagaggaa atattaaagg ttcactctag agacgttaaa
     1921 ttatcagacg atgtatcttt agaagaaata gcaaaatcta ctccaggtgc tgttggggca
     1981 gacttagcaa acatagttaa tgaagctgct ttaagagctg taaaacatgg aagaaagttt
     2041 gtaattcaag aagacttaga cgaagctgtt gaagttataa tagcaggtca agaaaagaga
     2101 gatagaatac tttcaccaaa agaaaagaaa atagttgcat atcatgaagt aggtcatgca
     2161 ttagttgcag ctttattaaa taatactgac ccagttcata agataacaat agttcctaga
     2221 acaatgggag ctttaggata cacaatgcaa cttccagagg aagaaaaata cttagtatct
     2281 aaggaagaaa tgatagatca aatatcagtt atgcttggtg gtagagcagc agaggaagta
     2341 gtatttaata gtataactac tggtgcatca aatgatattg aaagagctac tcaatcagct
     2401 agaaatatga taactattta tggtatgagt gagagatttg atatgatggc tttagaagct
     2461 atgagtaata gatacttaga tggaagacca gttagaaact gttcagaaac tactgctgcc
     2521 atagctgatg aagaagtttt acaagttatt aaaaaagctc atgaaaaatc aattaagatt
     2581 ttaattgaaa atagagagtt attagatgag ataacaggtg ttcttttaga taaagaaact
     2641 atcatgggtg acgaattcat ggaaatagtt tatggtaaat atccagaaaa gagagaagct
     2701 gatgaaaagg ctaaaaagga aattcaaagt ctaagagagc aagctttagc taagagaaaa
     2761 gaaaaagaag aagcaatcaa aaaagctaga gaagaagcat taagattaga agaagagcaa
     2821 agaaaacaag ctgaattaaa agctatgata gaagctcaag aagaggctgc aaaattatca
     2881 agagctaata atgaagcttt agattcatct aaagaaaatg aagaagttaa atctaatgtt
     2941 aatgatggag ctacagaaga aaagaaagat gatagctcta caaataataa agttgatggt
     3001 gaatagaaat aaggcgcata aaaatataga aaaaaatatt ttctatttca cttttagtgt
     3061 tagtttaaaa taatatacac tataatttta taagatgata aaatgtgcat gaagtttaaa
     3121 aatgcttcat gcactttttg acttttcatt tgtattttta atgagaggaa agttaatgtt
     3181 ataattgggt ataatgtaat agagaaagta agaaaggaag attctaatga ctgataaaaa
     3241 agatatagaa tttttagttg aagagaaaaa attagaggaa gtttctaata tattaaatga
     3301 agaaatgctt tcatatataa ataaaagaaa atttgtaaca caatatatat tagattatag
     3361 aaaaaatgca gttgaagaat ataaagatga tgaagataaa ttaatagagt actttgacca
     3421 tgaaagatat gtaaaagaac aagcctttac tacaatagat aaaaaattaa aggaattaac
     3481 aatcttaaag gaatcacctt actttggaag agttactttt aatgatttag agtttgatca
     3541 aaaggatact ttatatgttg gtagatttgg agtaactcca gagggaagct atgagcctgt
     3601 aatagtagat tggagagccc ctgtggcttc acttttttat catggtagtt taggtgaagc
     3661 aagttataca tcaccagatg gacctataaa atgtgatata gaaggtagaa gacagattat
     3721 agttaaaaaa ggagaattaa agggagtttt tgattcagct atagatgtaa aagatgatat
     3781 tttacaaatg gttctaagta ataatagttc tgataagctt aaagatgtaa taatgacaat
     3841 ccaacaagag caagatgaaa tcataagaaa agagagaact tctaatatag ttgtaaatgg
     3901 ggtagcaggt agtggaaaaa ctacaatagc acttcataga gtagcatacc ttttatataa
     3961 ctatagaaaa gaattagagg ataaagttct tatcttagga ccaaatggta tatttatgga
     4021 gtatatttca caagtattac ctagtcttgg ggaagtagga gtaaaacagg agacttttgc
     4081 tagttttgct ttaaaagaaa tggatagtga attatacata atgagttttg ataagtattt
     4141 agaaaaaata ctttcagagg ataaagagtt tatagaggat gctaaatata aaaattcata
     4201 tgagataatt aaagatttag ataacctagt aaaagatatg gataaggatt attttcacgt
     4261 tgaagatgta aaatactttg gtgatttagt tatttcaaaa gaagaaatag aagaaatgtt
     4321 taataaacat tatgagtata tgcctttatt cagaagaagt gaaaaaatta agagaataat
     4381 attatctaag ataaaagata agagagatga aaaagtttgg gagcttaatg aagaattaag
     4441 aaaagaaaag gaaaagttaa ctcctgaaga acttttaatt gaagaaaata acttagaatt
     4501 taaaagaaaa ttaagaataa gagaaattgt taaggaagta atggattcaa gagctaagtt
     4561 agatagttgg atttcaagag aagatgtttt agatatctat gatagattta atggaaataa
     4621 aaaagaatat acaataaatg atttagcacc aatattatac ttagcaataa aattagaagg
     4681 taaaaaagca acaaaggatt atagacatgt ggttatagat gaagctcaag attattcacc
     4741 acttcaattt aaggttgtaa gagaattaac aggaactaag tattttactg ttgtgggaga
     4801 cgtaaatcaa agacttatta aatattcaga cttagctcct atgatggaac taggaaaaat
     4861 atttgatgat gtaaatctag agatttataa cctaaataaa agttatagaa gcacttatga
     4921 aataatggaa tatgcaaata aatatcttga tgaagataga ataattccta tagttagaca
     4981 tggtaaacca gttgaggaaa ttgaattcca taataatgag gaactttcag aaagcattat
     5041 tgagtcttta aaagaatttt caaatgaagg cttagaaagt atagctatta taacaagaga
     5101 caaagaagag ttagaaaagg tgtataatct tataagcaat aaagtgcatt tagttaagtt
     5161 tgataacgaa gatgtattat ataaaggtgg aaatgttata ataccatctt actttgcaaa
     5221 gggattagaa tttgatgggg ttattattgt agataatggc tcatcaaagg atgaaaatga
     5281 agatctaata aaatatataa tgagtactag agctcttcat agattaaaag aaatacaatt
     5341 taaataattt tactttttaa aaggatttta taaaaataga ttttaatgaa agttataagc
     5401 atattttgag aatttctaaa ctagatattc tcattatact ccttgtaaac ttttattatt
     5461 aaacttttat aaaatccttt ttattttact aaaggagatt aagttttatt gctgtataaa
     5521 atttgtttaa cataagaata aaatgtataa aaacacaaaa tgaagatcta gaatcaaata
     5581 ataaatttga aaataaaaca cattaaaatt aattaaataa ctaatgaata tgttttctat
     5641 ttgagtatat agttacaata tacttattaa aaataatttt atattaaaaa tcaacttttg
     5701 caatatattt atattaaaaa acatattgca tttttattca ttaataatgt ataattattc
     5761 atgaagagag caataagcga ataaatatta gggggaatat atatatgaaa aataatatat
     5821 ttaagaagat gttgtcagtg gctatgattg gaggattgac tttaagttta gcaggatgtg
     5881 gggcaaaaac tgctaaagaa aatggatcta atgataaggt agctaagata aaagaaagtg
     5941 gaaagttagt agttggtctt agtgctgatt atgcacctta tgaattccat ataatgaaag
     6001 atggaaaaga ccaaattgtt ggtttagata tagacattgc aaaagaggtt gctaaaaatc
     6061 ttggggttga tttagaaatt aaagagatgg aatttggggc tataattcaa tctgttaaaa
     6121 atggaatgat tgatatgggg atttcaggaa taactcctga tgaaaaaaga aaagaagcag
     6181 tagatttttc agatatttat tatgaagcag agcaagggat attaataaat aaagataata
     6241 aagaatctat aaaaggaatt ggggatttaa aaggaaaaaa agttggtgct caaatggggt
     6301 ctattcaagc agagatagct aaaggaatag aaggagccga tgttaagctt ttagataatg
     6361 taaatacttt aattttagaa ttaaaaactg gtaagttaga tgctgtaatt actgaacttc
     6421 ctgttgctaa aatagcttca gaggttaatt ctgaattagc tgttgcagat gaggtgatta
     6481 aaaactcaga gggtggttca gccatagcaa tacaaaaagg aaataaagac ttagttgatg
     6541 aaatcaattc tacaattaaa gagttaaaag aaaatggaaa gatagataag tttacaaatg
     6601 atgctattga attagttcca tatcaaaaga aagaagaata aaatcaattt taataattag
     6661 ggggcaatac tatgggggga ttagacttca gttttttaaa agagtatttt ccagtgtttt
     6721 tggaaggtgc taaatggaca ttaataattt cagcaataac agttattttg ggagctttat
     6781 taggaacaat tttatgcttt atgaaacgtt tcaaattttc aatatttaaa gttaaaattt
     6841 taaggattat ttctactgct tatgtagaag ttataagagg aacacctctt ttattacaaa
     6901 tatatatagt ttattttgga ttaccttcct ttggaattca attagaggtt ttaccttcat
     6961 gtattattgc tatttcttta aattcagcag cttatgtttc agaaataata agagctggta
     7021 tagatgctgt tgataaggga caattagaag ccgctagaag tttaggaatg acagaaggaa
     7081 tggctatgag acttataata cttcctcaag ctataaaaaa tatacttcct gctataggaa
     7141 atgagtttgt tgccataata aaggaatcat ctatggcatc tacaattgga gtagcagaac
     7201 ttatgtattc atcaaaaata gttacagggg ctacttataa aggttttgaa ccacttattg
     7261 ttatagcaat gttttatttt gttatgacct ttagtttagg aagagttatg tcttatgtag
     7321 aaaagaggtt tagtaaatca tgataaatgt tagaaattta tataaaagtt ttggtaaaaa
     7381 tgaagtttta aaagatataa atgaaacaat aaaaaaggga gaagtagtag ttataatagg
     7441 accttcagga tcaggaaaaa gcacattttt aagatgctta aatttattag aggaacccac
     7501 ttcaggggta attaattttg aaggggaaga tataacagat aaaaatgttg atataaataa
     7561 aataagagaa aagatgggaa tggtttttca acaatttaat cttttcccac ataaaacagt
     7621 tatggagaat ttaacaatag gaccaacaaa aataaagaag atttctaatg gtgacgctgt
     7681 aaaaaaagga agtgaattat tagaaaaggt aggtctttta gataaaaaga atgtttatcc
     7741 aaattcactt tcaggaggac aaaaacagag aatagctata gcgagagctt tagctatgga
     7801 accagatgtt atgctttttg atgagccaac atctgctttg gaccctgaaa tggtaggaga
     7861 agttttagga gttatgaagt ctttagctaa agatggaatg accatggttg tagtaaccca
     7921 tgaaatgggc tttgcaaagg aagttggaga tagaatatta tttatggatg aaggaagaat
     7981 aattgaagaa ggtactccag aggatatatt tcaaaatcct aaaaattcaa gaactaaaga
     8041 ttttctatca aaagttttat aaatgccttt aagagattaa attttaatag atttagtctt
     8101 ttaaataaat taattagtac aaaaagagga tgtaaagaca aaaatcttta tgtcctcttt
     8161 tgtattttaa aataaaaatt gattattgaa taaatattaa ttatttttta atgaaaattt
     8221 tactgtaaat gttataatat ttaagtaaat attatataga aagatagtga ggaaatttta
     8281 tgaaggaaaa agtaaaaagt ttttttaagt gtaactggtt ttttattttc ttagtgataa
     8341 cacttcaaat aaaatcaatg ttacttttat ctatgcttag aactccgggg tctagaggaa
     8401 ttaattttga cttaatgtat tttactcccc ctgcttggtg ggctcatata gcaatagtta
     8461 cgttaatagc gagttttgtt tatctattta aaggtaaagg aagaatatgg gccggtatag
     8521 ttattgatat tctagtaaca attttatttg tggctgatat ttggtattac agagtaaatg
     8581 gaactttttt atcaataaga catataattg agccaggaat atttaatcct gtaggaaaga
     8641 gcttatttaa tttagctaaa gtagatgtag tgttcatagt tgattttata atattatttt
     8701 tagtttataa ctttacaggt ttaaaaaatg ttaaatataa aaataatata aaaactagat
     8761 taattgcatt tatatgcctt tttggaataa gtgcaacagt aataggggta tcacactact
     8821 atatagatat tcaaagaaaa tcagataaaa gttttttaag aatatcatgg gcgccttttc
     8881 aaacaattag tgattcaagt ccattaggat atcatggata tgatatttat tattatgcta
     8941 acagaaaaga aactttaact gatgctcaaa aaaatgaaat taaaacttgg tttgatgaaa
     9001 ataaagaaga tttaccagat aacaagtata aaggtatgct tcaaggaaaa aatgttatag
     9061 ccttgcaggt tgagtctctt gaaaactttg ttataggtaa aaaggtttat ggtcaagaga
     9121 ttactccaaa cataaataaa cttttaaaga atagtttata ttttgataat ataaaagaac
     9181 aaaacaattc aggaacaagt tcagattgcg atataatggt taatacatca atacttcctg
     9241 ttagagaagg aactacagta tttggttatc catgggctga atataatact ttacagaaaa
     9301 tattaaagag taagggatat tcaacagttt caacacatcc agaggttcct ggaaattgga
     9361 actgggcaga ggttcataag gcatttaaag ctgatgaaat atgggatgct catcagtttg
     9421 atcaaagtga aataatagga cttggaatgt cagatgaatc ttatttaaga caggttggag
     9481 aaagattaaa gagtcaaaaa caacctttct atacattctt agtaacatta acaagtcatg
     9541 gaccatttga tatgcctaag gataaacaat atttaaattt accagaagat ttaaatgaaa
     9601 atatgttagg tgcatatttc caaagtgtta gatatacaga tgaagctatt ggagaattta
     9661 taaatcaatt gaaagaagaa ggtcttttag acaatactgt aataatgatt tatggagatc
     9721 atggtggagt tcataaattc tatgaagata aaataaaaga tgctccttta gagggagatt
     9781 ggtggaaaga tgatgaaaag gaaatacctt tcttaatata caatccaagt attaatggag
     9841 agactatatc aaaagaaggt ggtcaaatag atttcttacc aactatagca taccttttag
     9901 gatttaatag agatactttt gataatacag caatgggaag agtattagta aatactaata
     9961 gaaatgcaag catattaaat aatggagaaa tagttggaaa tccaacacct gaagaaaaag
    10021 ctcatttaga gaaatcattt aatattgcag atatgattat acaaggaaat tattttaaaa
    10081 ataattaatt aaatttaaag ctattagcta gattactatt aaaatctaac taatagcttc
    10141 tttatatgta ttttttgaca aaatatttaa actattacca aattaaatat gttataataa
    10201 tgtttgtttt agaacttgaa aaataagtag tttataaatt tagattagtt agttaagttc
    10261 taataaaaaa ttgtggatat agttatataa ttaaaatata ataattttta ataaatatta
    10321 taaaatataa aaaaacaaat atgtcattac acatagggat ttaagtgaat aaatataaat
    10381 taggaggaaa aatggataag atattgttaa taattccagc ttttaatgaa agtgaaaact
    10441 tacctaaatt aatcgctagt ataaagaaag aatcttatga ttacgatgta ttaattataa
    10501 atgattattc aacggataat accgggaaag tagctaaaga gttaggggta aatgtaataa
    10561 atttaccatg taatttaggt attggaggtg ctgtacaaac tggatataaa tatgcatatg
    10621 aaaatgaata tgattatgca atacaagttg atggtgatgg tcagcatgat ccaatatatt
    10681 tagagttatt acataaaaaa ataaaggaag gatataattt tgtaattggt tcaaggttta
    10741 ttgaaaagaa agggtttcag tcaacattta taagaagagt agggattaaa tttttttatc
    10801 atttaataaa atttcttggt aaagaaaaaa taacagatgc aacatcagga tttagaatag
    10861 ctgataaaag tgtaataaaa ttatttagtc agtactatcc ttcggattat ccagaaccgg
    10921 agacaataat gctattaata aaaaataatt ttaaaatagc agaaattcca gttgtaatga
    10981 aagaaaggga gaatggaaaa tcatcaataa atattataaa atctatatac tatatgataa
    11041 aggttacatt ggcaataata ttatcatatt ttagtaagaa aaggagctat gtataaaatg
    11101 gatagagtat tacaatttat aattttaagt gtatgtattt tatttactat ttatattata
    11161 aagatgataa ataaagaaaa attagaacta aaatatgctt taacttggct tttttctagc
    11221 ttaggactaa taataattgc tgtatttcct caaatattag attgggtatc agttatttta
    11281 catatattat ctccagttaa tgctttattt ttaatagtga tattcttctt attagtaata
    11341 atttttacat taacagtggc aatatcaaag tctaaaaaac agataatagc aatatcacaa
    11401 gaagttggta tattaaaaga aaagattgaa agaggtgaga ataattaaag ctgtattttt
    11461 ggttttagta aactgtataa tgttagtttc aggacaaata ttatggaaaa taggattaaa
    11521 agatgctagc atgggaagcg taaaagacat aatattatta atgtttaata aatatgtttt
    11581 tagtggttta gttatttatg cattggctac aatttattgg ttttatataa taaaaacaaa
    11641 tgatttgagt aaggtttatc caatgcaaag tatgagttat ataatatccc tatttatgag
    11701 ttactatatt ttaaaagaga gtatatcaat taattcaata ttaggaacaa tagtaatact
    11761 tttaggtgta tttattatag ctatgaaata attaaggagg agtttatgac tttttgggaa
    11821 aaagataaaa gatatagata tacattgtgg ttatgtatat tagctatgtt tgttatagga
    11881 acagtatgta cattaaaata tggtaattac tttttactag gggatttaga taagttaaac
    11941 aacgatgatg taagatattt acatacagct aaagtattag ctgaacaagg gaagttggta
    12001 tatcataata tggatcctac tttgtttatt atgccaggat acccaatttt tatagcacta
    12061 atagtaaaaa tttttggaag tggaagttta ggtataatag caataagaat atctcaatta
    12121 gtacttcaat gtgtttgctt atatatatta tattttttaa caaaagaatt agtaaataaa
    12181 aaaacagcaa taatagcatg tattcttaca gtattatatt tgccagaata tgtggcagca
    12241 aatcttatat taacggaagt attatataag actttatata tgcttttatt ttatttttct
    12301 ataattgcca taagaaaaaa taaaactaaa tactatgttt tttcaggtat aagttgggct
    12361 ttagtatgtt tggtaagacc aaatgcagca gcatttccat tatttataat aattttttgg
    12421 atagttaata agtattcaat taaggatatg ataaaataca catccatagt atttgtaata
    12481 tttgtaactc ttttttctcc atggtggatt agaaattaca aattaacaaa taaatttgtt
    12541 ttatttacag aatcatcagc aaatcctaaa ttattaggta catttataag atggggagct
    12601 cctagttttt ataaggatat accaaaagaa tataaatatg atgaattttt aaatgacgaa
    12661 tatctaacag aagatgaaca aaataattta gcaaattata tgattaaaag aagttttcaa
    12721 gaagaacctt taaagtacac ttattggtac actttaggta aaactgaaga gctttataag
    12781 gaagcatatt attggaaacc tatatttaga gttaatgaca caaggatgaa ttttacacat
    12841 atttcatata taactcttgg aatattagga attattgcta tgattagaag aaagattaaa
    12901 ggtggaaaaa tgttaatagt gtttttactt ataaatactg ccgtgtatct tccttttata
    12961 actttctcaa gatatggata tccaaatata tttgtattta taattggagc tgcatatact
    13021 ttgaatgttt tattttgtaa ggatgaaata caaagtgaaa aaagcctaat ttaggggtgt
    13081 gaagttacat gaaaaaaaat gaatatagat ttttatcaat acttaataac aaatttttat
    13141 tatttttcct cttttcttta ttgataataa taaaagaagt attttttact tggatatggt
    13201 caagtagtga tagtattgct aaattgcaaa tttttaatat gtatatgtac tggccaaagc
    13261 tattaataca tatagttttt gctatgacta ttgcaagtgg aattttttta tttaataaaa
    13321 atggaagaat aatttatata ttaatagcag atattgttat aacaattctt atgtttttag
    13381 atatttcata ttatagaaat tacgggaatt ttttatcaat aagacattta tttcatgaag
    13441 agttgtttaa tcctttaaat aaggaattat ttaattttta taaaagagat attcttcttt
    13501 taattgattt tataatttta gttcctctat caattttttc attaaagaat gatagtggta
    13561 aaaagagtag aagaagtata aagatattta tactaagctg gataataaat ggaataatta
    13621 tatacacaag ccattcttta gtagatataa aaggggttac taatggtaaa ttaacattgt
    13681 ttgaaaaatc atttgctcca caagttaata tggatgattt gggaatggta ggatatcatg
    13741 aatatgactt aacaagctat attttaaaaa aagacaaaaa gctaagtaca gaagaaaagg
    13801 ttgaaataaa taaatggttt gaagagaata aagagacttt acctgataat aaatataaag
    13861 gacttggtaa aggaaaaaat cttataatta tacagtggga atctttagaa aattttgcta
    13921 ttaattataa agttgatggt caagaaataa ctcctaacat gaacaaatta ttaagtaatt
    13981 cactatattt tgacaatatt tatgagcaaa ataaaaatgg gacaacttct gatgctgaat
    14041 taatggctaa tacatcatta ttaccaataa gtgaaagtgc gtattttata caatatccat
    14101 ggaaaaaaca aaatacactt caaagacttt tagagaaaca cgggtataat acagctacag
    14161 cgattgctga taagggtgga gtatggaact ggttagaaaa tcataaaagt tttggtgtac
    14221 agactatatg ggatagcagt tatttcaata gagatgaatt gataggatca aatattacag
    14281 acggaagttt atttagacaa acagaagaaa aaataaaaac attaaaaaga ccatattatt
    14341 tgtttatggc aacagcaaca tctcacggtc catttgatct tcctactaac tatagaggac
    14401 ttaaattacc taaagaaatt gatgatacta aattaggtgg ttatcttcaa agtttaagat
    14461 atactgataa aatgcttgga gaattcttga ataaacttaa aggtgatgga gtgctagata
    14521 atagtattat tgtcatttat ggagatcatg gaggaataaa taaatattat aaaaaagagt
    14581 tagaaaatat agattttgct aataacaatt ggaagcagga atacttaaag gtacctatgt
    14641 tgatatataa tccagaaatt aaaggtgaag ttataaatac atatggtgga ttggtagatc
    14701 ttttacctac tgttggatat atcatgggag ttgacaaaag tgattttgaa aaaacagcaa
    14761 tgggaagagt tttagtaaat acgaatgtaa atgctacaat aaattcaagt ggtcaaattt
    14821 taggtaatcc taaagatgaa aaagagataa ggcatctaca agatatgtat aaaattagta
    14881 ataatataat agagagcaat tattttaata attaactaaa tagaaaaaaa taaaaataca
    14941 gatagtttta attaaaacca tctgtatttt tttaacaagt aaaatcttta tcaatagtta
    15001 ttatgcagtt atattgaggg tgagtttctt ttactgcctt tataatttct tctttaagct
    15061 cactttcaga cataactttt cttagttttt ctgggttaac aacaatatcg aatataagat
    15121 ttttcttgtt accatgacca acaattctaa aatcgtgcat tgattctatt agtgggttat
    15181 attttattat tttgtctatt tcttctttag cctgttttat ttcatcatct tctaaacaaa
    15241 tagggtccat atgaattacc aaataaatct ttagtttttt agaaatttct ctttcagcca
    15301 tatctataat atcatgtatt ttcataacat ttatgtcaga aggtatttct gcatgaattg
    15361 aagccatgca tttacctaca ccatagttat gaattataag atcatgaact cctattatat
    15421 gatcatatga tagaaccatt tctctaatat ttttagcaag ctctggatca ggtgcttctc
    15481 ctaaaagagg atttatagtt tcttttacta aagtaaatcc agcatataga atagctaatg
    15541 aaacaataac acctatatat ccatcaatag ggaatgtagt aaattttgca gctaaaaatg
    15601 atataacaac acatgttgaa gtaaatacat ctcctaaagc atcaactgaa gcagccttaa
    15661 gagctgaaga atttattcta ttacctgtta atttattaaa tctacttaac caaaatttaa
    15721 agaatattga tactataagt agaataaaag gaactacttc aaaggttaca ggtgttggat
    15781 taagaattct ttcaattgat gatttggtga attgaaaacc tactaacatt accatgaatg
    15841 ctacgattag agctgaaata tattcaattc taccatgtcc aaatggatgt tctttatcag
    15901 ctggcatttt agctaattta aaacctatca tggttattac agaagagcca gcatcagata
    15961 agttattaaa ggcgtctgct gttatagcta tacttgctga aattaatccc acagaaaatt
    16021 ttattaagaa taaaattata ttaacaatta ttccaactat actagctaag taaccataag
    16081 agcttctaac actttcattt tctgtgtttt tgtaatcctt aatgaaggtt tttattataa
    16141 gatttgaaaa catattaatc atctctccat ttttattaag ttttaaatta agtttatatt
    16201 aaattaaaaa ataattgtta tagttgatta aaaaataaag catataagtt atataaattt
    16261 attcaactaa atctcattat attatatcat aacattttat gttgaaatag aatataaaat
    16321 attataaata aatctagaat tattaatgca tagttatttg ttaataaatg gagatattat
    16381 tatataacta aaatattgac taaattaata aaaaattaag aggaaatttt ttaaggtatt
    16441 ttaatgtttt attaattagg tattttgtgg taaagtataa taaaaagtat ctattttata
    16501 aagaaaggtg agatacatat ggaaagagtt tttgcaaatg aattaattaa ttttttatat
    16561 gaaagtccaa cagcttttca tgcagtaaag aatgttaagg acactttaga gtttgaaggc
    16621 tttaaagaat taaaagaggc tgatagatgg aatctagaag ttgaaggaaa gtattttgta
    16681 actaaaaatg attcagcttt aattgctttt agagttggta aaggtgatat tgctgaaaat
    16741 ggatttaaaa taattggtgc tcatactgat tcaccaggat ttagaataaa accaaatcca
    16801 gagattacag ttgaaaatac ttatgtaaaa ttaaatacag aagtttacgg aggaccaata
    16861 ctaaatactt ggtttgatag accactagct ttagctggaa gggtatcatt agtaagtaaa
    16921 aacccattaa aaccagtaaa taaaatagtt aatataaata gacctatact tataattcca
    16981 aacttagcta ttcatatgaa tagagaagtt aatgaggggt ataaaataaa tagacaaaag
    17041 gatacattac ctttattatc acttgttaat gaaactttag aaaaaggtaa ttatttagtt
    17101 gaacttttag caaaagagtt atattgtgaa aaagaggata ttcttgattt tgaattatat
    17161 ccatatgaat atgaaaaagg ttgcttaatg ggattaaatg aagagtttat atcttcagga
    17221 agattagatg atttatcaat ggttcatgca ggtattaaag cattaatgga tgctgaggta
    17281 tcacaagcta ctaatgtaat ggtatgtttt gataatgaag aggttggaag tgctactaaa
    17341 caaggtggag attcagattt cttaaaaaca atattagaaa gaatagtcct agctttaggt
    17401 aaagatagag aggatttctt aagatcatta tcagaatcat ttatgatttc agcagattta
    17461 gctcatgctg ttcatccaaa tttaggagaa aaacacgatc ctgtagttag acctgtttta
    17521 ggaaaaggac ctgtaataaa aatagcagca tcacaaagct atacaagtga tagtgattca
    17581 tcagctgttt atgaaatgat atgtagaaat gcaggagtta atgttcaaaa gtttgttaat
    17641 agatcagata tgagaggtgg ttcaactata ggaccaatat catcaactca tttaccaata
    17701 agatcagttg atatgggaac tccaatatta gcaatgcatt caataagaga gtttggtgct
    17761 gttaaagatc attttgattg tataaagtca ttcactgaat tctttagaat ttagtctatt
    17821 aataattttc aatatattta gttaatataa tttttattaa ttataacaaa agtgggatgt
    17881 agaaatttac atcccatttt ttatatttaa atatcatcgt cgtctagcat atcgccttct
    17941 atatagtcaa attcactata ggatatattg ttgcaaacta tgtcatcttc taatgggttt
    18001 ggcaatttag tgtaagtttt accagtctta gaataagaaa ctaaatcatg tttttctgga
    18061 ctataaaagt ctctatcttt tggaggatat tttttactca tttttatttc ccctttcaaa
    18121 ttttaatttt gagttttgtt ttagattaat gatttaattt aaactagata aattaaagtt
    18181 acattaataa aaacaaatta ttaatatagc tataaattag agttttaatc tctaaatttt
    18241 tagcttaaat ttatttttcc caaaataaat ttagttatta agctaaaatt agagtttgct
    18301 tggtaattag tatagtttta ggaaacctat gtgttatttt gttaaaacta ttattaataa
    18361 gttttaagta gcttatattt atttaaagtg gaattaataa agttgaaaaa agaaatacta
    18421 tactttagga tggtataata agaataaaaa atagaaaata catagttggt agtttggagg
    18481 agaatttaat tatgtcatat aaattaatat gtatagatat ggatggaaca ttattaaata
    18541 ataaacatga aattagtgaa agaaataaag aggctataaa aaaagctaca gagagaggcg
    18601 taaaggtagc tgtaacaaca ggtagattat ttacctcagc taaatattat gcaggacttt
    18661 taggagttaa aacccctata atatcatgta atggtgcttt tataagagaa aaggatgaag
    18721 ataaagtaat atatgaatct gttttaaatg aggatcaact agatagaata tataatgtca
    18781 taaaaaaata tgatattaat atggcctatt ttaatacacc aaacactgta atttctgaaa
    18841 agattgttcc agaagatcat ggctataagg ttatgaatag aatgatggga gaatcaaaag
    18901 aaaaggtaat gttctcagaa ggaatagatt ttagagaagc ttttaaaact catgggaaac
    18961 atatacttaa ggctatttgt atagaaaatg atagtaataa gcttaatgat ttatttaagg
    19021 caaaagaaga attaaaaaaa tacgaagact tagaagttgt aagttcttca ccaagtaact
    19081 ttgaagtaat gaataaaggt acttcaaaag gacgtgcagt aaaagtttta gctgatatat
    19141 taaatataaa cagagaagag gttatgtgtt taggagatag tgaaaatgat cttagtatga
    19201 tagagtttgc aggtcttgga gtagctatgg gaaatgcaga agagttttta aaagaaaaag
    19261 ctgattacat aacagataca aatgaaaatg atggagtagc taaggctata gaaaagtttg
    19321 tattagatta atttaaccat atttttttat aatatagaag acactaattt tatatgagat
    19381 taagtgaaaa gttaagtttt tttatttagt attttaaaaa tattttctta aataaagaga
    19441 gactaaaatc gtaaaattta tgattttagt ctctttattt tcataaaaat ttattttgca
    19501 aagtagaagt ttctataagt ttctctatat tatttatttt attccttggt tgtggttgtt
    19561 ttggattctt tttttgttac tttagaagaa ccttttttag ttgtagtggt agttgtggta
    19621 gtagttgtga ccactgttgt aacaatagta gtggaatcat ctaaactttt atcctctttt
    19681 gataaggctg gtttaaatgg ccaccaattt gcctttccaa atatttttac cattgctgga
    19741 ataaatagag gaagaacaaa gacaacatat aaaagtatgc ctgttagaac caccatggaa
    19801 atttcaatta aggataatac tccagatgga agtaaggctg caaaggtacc actaaggatt
    19861 attacggcag atattattac cccacccata cttttcattg ttcttattat acgttctttt
    19921 atagaaccat taagatcttg gttaaatctt gtcattataa atattgaata atctatacct
    19981 aaagccatta aaactacaaa accaaagaat gatgttatcc atgatatacc tggatatccc
    20041 atgattttag tgacaacaaa ttgtattaca cttattgaag taaaataagt tagtaataaa
    20101 gaaatcatta agtaaattgg cattgttaaa gatcttaata taataattaa ggtaataaat
    20161 actccaacta ttacaaatat taaagcttta tcataatcag agtcagccat ccttttagtt
    20221 tcgtatgttg tactagccat tccaccaata cctatattag cattttctaa cttagttcct
    20281 ttaactccat cttgaacaag agatttaaga ttaggaattt cattaactgc ttcattagaa
    20341 taaggattct ttgtagtaat tacatttatt actgctaatt taccatctga gctcatatat
    20401 tgatttaggg attttttaaa ttcatcatta ctaaatacct caattggaac acaaattcct
    20461 gaaagattat aagatagatt tgaccaatca tttataaatc cattagcata cttaagacca
    20521 ttttgaattt cagttaatcc attagaacca gcctcaagtc cattagataa ttcatttatt
    20581 tggccagaaa attctttgaa accatcttgg attttagatt gaccattagc tatttggttt
    20641 aaggcatttg aaatttcagg aactttagat gaaatttctt ctccccctgc taaggatttg
    20701 ccaagaccac tatctaaagc ctttaatcca ttttcaattt cagaaattcc agaattaaat
    20761 ttatctaaat catttattac agtatccttt ttgctattta aagtatttat tagaactttt
    20821 aatgcgccta tgctttcgtt aattgcttta atctctggag tattattaaa agttccttgt
    20881 aaattattag agttattatt taaattatta atatcatcag agaatttttt gaaattttca
    20941 tcatttaaaa cctctggatg tgactttgaa tattcactta aatgagaatt caatgatgaa
    21001 agagattctt taagtttact agtatctaca ttaactgaat ttggattacc tgttaaggaa
    21061 ctaattttgt ttaaattttc aagaacatca ttagcagaat taactatggc actataatct
    21121 tcttcaagtt gagatttact tagttttaaa gaattaactt tatctaaggc accgtctatg
    21181 gaatctttta gctctgtagc tccattatgt tcttcacgaa tactttccac aagtttatta
    21241 agagcacttt gaagcttgga aactccagtt tcagtttttt tagtaccttc ttgaagctta
    21301 gctgtacctt cttcagcccc ctttaacttg ctctttgatg aatctatttt tttactagca
    21361 tcacttaatc catcatttat tttgtttaat ccattatttg ccttatctat accctgtgaa
    21421 acttgaccag cttggtcatt tacataaatt tcatcaagac gaactcctgt tggtctagta
    21481 acacttaata ctgattttat atcattacta gagttttgta gattattaga aagtttttct
    21541 attaaggcta catattcagg ctttctcata tcatcatcat tttctatgta tattgaaaca
    21601 ggagatgctt gtcctatacc aaagtcatct gctattatat taaatccttc cttagcagag
    21661 taagtatccc caatttcatt taaattgtta aatgattgat gaaagttatt aaataaaata
    21721 ggaagtaaaa gtatggtgaa tatagctcca agtataggaa ttggtttact aaatgcttta
    21781 ttagctaact tagcccaaag tctattttct tctgctttaa aggttccttt aacaggccag
    21841 aaaatattct ttcctaatat aatcattact gctgggaaaa ttgtaaataa ggctgcaatt
    21901 aaaaataata taccaattgc tacagctacg gcacttctat ataaattaaa tccaataaaa
    21961 tttaaggatg caaaaacaac aaatactgga gtagcactag ataaaactgt tttaccagca
    22021 gttttaaagg ttgtttttac agaatcaagt ttattatttc cattagctaa ttcctcttta
    22081 aatctattaa gtagcaaaat agagtaatca gttccaatac cgaacattat acaaatcata
    22141 aatatttgag tgtagtttga aagaggaaaa ttaaatttat ctgctaaaat cccaacaaca
    22201 gattgagctc ctaaataact tattcctaca gtaagtaaag gaataaatgg agtaactact
    22261 gatttaaaca ataatataag tactatgaat ataaatatta cagttattgt ttgagttttg
    22321 tttagtcctt tttgtgcagc cacattaaca tcattctcta taagactttg tcctgtaaca
    22381 taggtttcaa cccctggagt ttttaattca tcatttaaag ttttttctat tgtacttaca
    22441 gaggaatcct ttggatttac tgtcattaag gctataatag ttttaccatt ttcagagtta
    22501 agttgatttt ttagctgagg agtatcaaaa ctatcattta tactctgaat ataatcacta
    22561 ttttcttttg atttcaatct atcaatagta tcctttatat tttttagatt ttcatctgtt
    22621 aatttattac ttgagtgaaa aacagctata tagctatctt tgctattaaa tttattatct
    22681 gattggagga tatctgaaat tttagaagaa taatcatcag gtaattgtat tcctcctttt
    22741 tcactaacta gctttgatag ggaaggggca gttaaaaata aaaatgctac aactgcacac
    22801 catattatta aaataaaaaa gctaagtggt ttattaagtt taatcttttt aagtatatta
    22861 tctttcatat attcccttct ttcttttacc actttaatta acttagttaa taaaaaatta
    22921 ataatttcat taagtttttt agatttgttt taaattaaat ttcaactgaa gtagaaggtg
    22981 ttctttaatt aaaagtaatg ttgctataaa tttatcatag cacacattaa gtttaaatat
    23041 gaactaagtg ttaacttgtt aaatgtaaaa aaacacatga ttctttaaga gttttcttaa
    23101 aatcatgtgt tttctttaaa ataatgtatt agtcgttaga tttaccaaca cctgaaagta
    23161 ttattccagg gttgtgatct atagcccatg aaatacccca gtcattttgg aatataagta
    23221 gatttctatc tttaaaatct ttaactttct ttgtatcaga agttaagttt agtgaatcta
    23281 catctatatt atcattttca atccatctaa catatctaaa tggaagtctt tccattttta
    23341 tatcaacatt gtattcattt tttaatctat attctaaaac ttcaaattga agaacaccaa
    23401 caacaccaac tatgatttct tccataccga tgtgtaattc tttaaatacc tgaatagcac
    23461 cttcttgagc tatttgagta acacctttta tgaattgttt tcttttcata gtatctacag
    23521 gtttaactct agcaaagtgt tcaggagcaa atgtaggaat accttcaaat ttgaatttct
    23581 taggatttaa gcataaagta tctcctattg agaatatacc tggatcaaat acccctatga
    23641 tatctccagc gtaagcttct tcaacaacct ctctatcttg agctaagaat tgttgaggtt
    23701 gagcaagttt aactttctta ccaccttgaa cgtggaaaac atcttttcct ttttcaaatt
    23761 taccagaaca tattctcatg aaagcaagtc tatctctatg agctttattc atgttagctt
    23821 gtattttaaa tacgaaagct gagaatggtt cagtcatagg atctatttca cctattgatg
    23881 aatttcttgg aagtggagaa gtagttagtt ttaagaagtc atttaaaaat ggctcaaccc
    23941 caaagtttgt aagagctgat ccaaagaata caggagttaa ttctcctttt ctaactttct
    24001 ctaaatcaaa ttcgtcacca gctatatcta aaagttcaac atcttccatt aacttattat
    24061 gtaaatcttc acctagaagt tctttaaatt taggatcttc taagtcacca gttatagttg
    24121 aaacctcgct ttgtccgtga tttccaccat caaacaagtg aattgtgttt gtagctctat
    24181 cataaacacc tttaaagtga cttccacatc ctattggcca attcattggg taagatttta
    24241 taccaagttc actttcaata tcttctagaa gttggaatgg atctctagtt tctctatcca
    24301 ttttgtttac aaaagtaaat ataggaattt ctcttaatga agcaacttgg aatagttttc
    24361 ttgtttgatc ctcaacccct ttagctccat caataaccat aacagctgag tcggcagcca
    24421 taagtgttct atatgtatct tctgagaagt cttggtgacc aggagtatct agtatattta
    24481 tacaatatcc accatagtta aattgcataa cagatgaagt aacagagata cctctttgct
    24541 tttctatttc catccagtca gaaactgcgt gtgtagcagc ttttctagcc tttacagaac
    24601 cagccattct aatagctcct ccataaagta agaatttttc agtaagagta gttttaccag
    24661 catcagggtg agatatgatg gcaaaggttc ttcttttctc tatttcctta attaaatcgc
    24721 tcaatctaat ttcctcctta atttttttat gtatattaaa atattaacta ttttccttca
    24781 ttaataatat ataaatatga aagattaaga aaataattgt tttatctctt aaatagccta
    24841 gactattata tcataaaata actatcaaat taataaatac ttttaagtaa aaaaataggt
    24901 catattctta acctttaata tgacctattt tatatatttt attttgaatt tttcttttta
    24961 agttctctca tagtaggaag tgcatcttta ttatctaatc caacaataga taatatttgt
    25021 ttagctctat ccttagttga accttctata gttgtgtatt taacattctc gctatctaaa
    25081 aattgcttta atgaattatc aacggcagag gcagttgatt catcttgcca tctaatacca
    25141 tcttgagtat aaccaaattc tctaggaaca aagaatatat gatcatactt ttgaagatct
    25201 ttaagagcta aatggtaaag atcttttagt atttcaattt cttttacaga tcttttttta
    25261 tcacctagca taaatcttcc ataaatatat ggtacgaaag ttgcataatc tgttaaagca
    25321 taatcaaatt gatttaatga ttcttctaaa cgaagttgtc ctaaataaat accgtattgt
    25381 tcagaaatag tttctatcat acctttttct ttaaggtatt ctgtactata ttcagttgcg
    25441 gctcccacat ctaagcctct gattttaaat tcaacataaa gttgttgtat taaagtagtt
    25501 tttccacatc ttggaccacc aagtattgca attctaactg gcatattaaa cccccattaa
    25561 attatgtatt tataataatt ttgaaaatag taaaataatt tatatttaat gtatttttta
    25621 gtcaaatcta ttatatcaat taaatcgaaa gctctaataa atgacaaatt tcattatatc
    25681 aacatgatag aagtatttca atattttaaa ttctttaaaa ataattaata aaaatttcag
    25741 tgcacactaa taataagaac aaattattac aatttatttg caataatttg aaaagaaata
    25801 atcatatgat ataatcttaa atgtatatgt caaaaaataa tgaaataaca taacaatttt
    25861 gatataatta cctaatatca aatgaaaaaa ttcaaaatat attttatata acgtttaaga
    25921 ggtgaataaa tgtataaatt agtagctata gatatggatg gaactttatt gaaagaagat
    25981 aaaactatat ctgagaaaac aaaggaagca ataaaaaaag caagagaaaa aaatgtaaaa
    26041 gtagttttag caacaggaag accagtagat ggagtaaaaa gatatttaga agaattagat
    26101 ttatttcata atgatgagta tgttttaact tttaacggag ctatagttaa ggaaatagga
    26161 cctgatagag taatttgtag agatacttta aaaggtagcg atttaaaata tttatatgaa
    26221 gtaagcaaaa atgctggagt taatatacat gctttttcta attttggctg tgtaactcct
    26281 aagatgaata aatatacaga attagaaggt agaataaacg gaatagaagt ttgtgaagct
    26341 gattataatg agatcaaaga agatgaagaa ataataaaaa taatgatgat tgatgaacca
    26401 gaagttctag aagaaggaat taagaagtta ccagaagaag tatatgaaaa atatactgta
    26461 gtaagaagtg ctccttattt cttagagttt ttaagtaaga cttgtaataa aggagaaggg
    26521 gttaaatcac tagctgaaag cttaggtata aaaagagaag aagttatagc aattggagac
    26581 gctggaaatg atcttcatat gatagagtat gcaggacttg gagttgctat gggaaatgct
    26641 tttgaagaag taaaagaaaa agctgatttt attactaaaa gtaatgaaga agatggcgta
    26701 gcttttgttt ttgaaaaatt tgttttatag atttcaataa aaatgataaa aattaagggg
    26761 gattttatgg aaagtataaa tgatattcca attaaatcag agtgtagtaa tgaaaaaagc
    26821 tatctatttt ataatgttgc aaaaagatgt tttgacttaa tatgttcgtt aattgcgtta
    26881 atattatttt caccaatatt tttaatttta tatatatggg tgaaggttga ttctaaagga
    26941 ccagcatttt ttgggcataa aagaattggt aaagacagaa aatatataga tgtttataaa
    27001 tttagatcca tggtttctaa tgcaaaagag gtttttgata attttacacc ggagcaaaaa
    27061 gctgaatttg aaaagaattt caaattagat gatgatccaa gaataactaa atgtggagct
    27121 tttttaagaa agacaagctt agatgaatta cctcaattaa taaatattat taaaggagat
    27181 atgagtatcg ttggaccaag accaatagta gataaagaag ttgaaaagta tggtgaatat
    27241 gcagacaagc ttttcagtgt tacaccagga ttaactggtt attggcaagc aaatggaaga
    27301 agtgacacaa catatgaaga aagagtacaa atggatatgt actatataga taatagatct
    27361 ttatggcttg atattaagat aatatttaag acggcaatat cagttataaa aaaagaagga
    27421 gctgtataga tgaagatatc tataatttgt ccattgtata atgctgaaaa ttatataatg
    27481 agtttacaca ataaaatatt agaacaagaa aaaccagagg gtacagaagt agaagtttca
    27541 tatgttttaa ctaaatgtaa ggataaaaca gaagaaatgt taaaaaatat gaatttacat
    27601 tatacactta tagaaccaaa ggatttttca cattcaaaaa ctagggagat gatggctttt
    27661 aagtcaacag gagatattat agtttttata tcacaagatg taatgatgaa gaataacttt
    27721 tggcttaaaa atttagtaca acctattata gatggggaat gtgaagcttc atttagtaga
    27781 caactttgta ctaataatac tatagaaaaa tatataagag aaaagaatta tcctgaagaa
    27841 tcaagagtgg tttctaaaaa ggatattaat aaatatggat tattaacatt tttctattca
    27901 gatgcatcat cagctgttaa aacaagtgta tataaagaat taaatggata tgacaataaa
    27961 agacttttaa taaatgaaga tatgtatttt gctgaaaagc ttatacaagc tggatataga
    28021 ataaagtatt gctcagattc tgaagtttat cattctcatg tatttactct taaatcattg
    28081 tatgaaagat actttgatac tggagttttc tttagacaaa actcacaatt tttaaactat
    28141 aaaggaaata agagtggtaa agatttggct attcatgtat taaaaagatc attagaagat
    28201 aaaaacttta aggttacatt aaatatatta cctaattttg gagcgagatt tattggaagt
    28261 ttcttaggaa agaaatatga aagtttacct agaagttttg ttatggcttc ttcaatgagt
    28321 aaaaaggtat atagtcaaat ttagtattaa ggtagaaggt gagaagatga aaggaatcat
    28381 attagccggt ggaagtggga caagattata tccagtaaca aaggcaatgt caaagcaaat
    28441 ggtaccaata tatgataagc caatgatata ttacccaatg tcagtattaa tgttagctgg
    28501 aataagagag gtattaataa tatcaacaga aagagattta cctaatttta aagagttatt
    28561 taaagatgga agtgaattag gtttaaaaat agaatacaag gttcaagaag caccgaatgg
    28621 acttgcagaa gcattcataa ttggagaaga attcatagga gatgacaatg tagctatgat
    28681 tttaggagac aatatattct atggacaaaa tttctcaagt aatttaaagg aagccgctga
    28741 tttagaaaag ggtgctatgg tatttggata ttatgttcaa gatcctaagg cctttggagt
    28801 agtagaattt gatgagaatg gaaaggtaat ttctttagaa gaaaagccag aacatccaaa
    28861 atcaaagtat gcagttccag gactttattt ctatgacaat acagtaatag aaaaggcaaa
    28921 aggattaaag ccatctgcaa gaggagaact agaaataaca gatttaaata gattatatat
    28981 ggaagaggga actttaaaag ttaatttgtt tggtagggga atggcttggt tagatacagg
    29041 aactcatgct tcaatgttac aagcatctaa ctttgttgag gctgttcaaa atactcaagg
    29101 tacatatata gcttgtttag aagagattgc ttatagaaaa ggatggattt cttcagagaa
    29161 ggtattaaac ttagctaaac cattaatgaa aactggttat ggaaagtatt tagttgatat
    29221 agttgaagag ttagaacaaa aaaaataata gagagatatt agacgattat ataaatttac
    29281 ataatataac aaattcttat attatgtaaa tttttttgaa ctttatatac aatttattga
    29341 aagtggtgaa aagatgagta actttaaatt tatagaaacc aaaataaaag atctatatat
    29401 tatagaacct aaagtttttg gagatgaaag aggatatttc atggaaacat atagtgaaaa
    29461 agcttttgaa gaagctggac ttactatgaa atttgttcaa gataatgaat caaaatctaa
    29521 aaagggtgtt ttaagaggat tacatttcca aactaagcat actcaaggaa aacttgttag
    29581 agtaactcaa ggagaagttt gggatgtagc tgtagactta agaaaaggat cacctacatt
    29641 tggacaatgg gaaggtgttc atttaagtgc agaaaataaa agacaattct atgttccaga
    29701 gggatttgct catggatttg tagttttatc tgatgaagct gtatttaact ataaatgtac
    29761 agatttttat gctccagaat atgatagtgg acttctttgg aatgacaaag atgttaatgt
    29821 agaatggcct ttagaaggaa tagaagaaat aatcttatca gaaaaagata agaaacaaaa
    29881 aactttaaaa gaattagata ttccttttgt atatgaaggt taatgggagg aaatctatga
    29941 agatattaat aacaggttca aatggacaat taggaaacga acttcaatca ataataaaaa
    30001 gtggaaaagc agaaataggt tcagttagtg aaaatataaa aaactcagaa gttagagctt
    30061 tggatgttga taagttagat ataactagct tagaaaatgt taaaaatgta cttaatgaag
    30121 aaaagccaga tgtagtaata aactgtgcag cagcaacaaa tgttgatggg tgtgaaagta
    30181 atgaagattt tgcatttaaa gttaattctt taggagcaag aaatttagct atggtttgtg
    30241 aagaaattgg agctaaatta gtacaagttt caacagacta tgtatttagt ggtgttggtg
    30301 aaaaaccatt aactgagttc gatttaactg caccttatag tgtttacgga aaaacaaaac
    30361 ttttaggtga aaattatgtt aaagagtttt gttcaaaata ttatatagtt agaactgctt
    30421 ggttatatgg atatgttgga cataactttg tttacacaat gatgagatta ggaaaagaga
    30481 aagaatcatt aaatgtagtt aatgatcaaa aaggaaatcc aactcatgca aatgacttag
    30541 cgtatcatat attaaagctt atagaaacag aagaatatgg aatatatcat tgtacaggaa
    30601 aaggggaatg tacttggtat gattttgcta aagaaataat ggagttagct ggcaataagt
    30661 gcattgttaa tccatgtact tcagaagaat ataaaacacc agctaaaaga ccagaatatt
    30721 catcattaga taatatgatg cttagatgta caattggtga tgaaatgaga gattggaagg
    30781 aagctttaaa aagctttttt gaaaatcaac aaaagaatca ataaaagtta ttatagttag
    30841 gagagaatta aatgaaaact tacttagtta caggtggagc aggatttata ggatctaact
    30901 ttgtattata tatgttaaat aaatacgaag atataaaaat aataaactta gataaattaa
    30961 cttatgcagg aaacttagaa aatttaaaat caatagaaaa tgatgaaaga catatattcg
    31021 ttcaaggaga tatatgtgat aaagaattag tttcaagttt atttgaaaaa tatgaaatag
    31081 actatgtagc tcactttgca gcagaatcac atgttgatag aagtataaga gagccagaag
    31141 tatttgctaa aactaatgta ttaggaacag taaatttact taactgtgct aaaaatgcat
    31201 gggaaacaga agaaggatgg aaagaaggag ttaaattcct acatgtttca acagatgaag
    31261 tttatggttc attaggagaa acaggatact ttatggaaac aacaccatta gatccacata
    31321 gcccatattc atcaagtaaa gcaagctcag atttaatggt taaggcttat gcagatacat
    31381 ataaaatgcc agtaaacata acaagatgct caaataacta tggaccattc caatttccag
    31441 aaaaacttat accacttcta ataaataact gtctaaatca taaggattta ccagtttacg
    31501 gagatggaat gaatataaga gactggctat tcgttgaaga tcatgcaaaa gcaatagata
    31561 tggtaataaa cggaggaaga cttggagaag tttataatgt tggtggtcat aatgaaagaa
    31621 ctaacattca aatagtaaaa acagttatag aatacttaca tgataatgta gatgaagaaa
    31681 taactgaaaa cttaatcaag tatgttgaag atagaaaagg tcatgataga agatacggaa
    31741 tagatccaac aaagataaaa gaagagttag gatggtatcc agaaacaact tttgaagttg
    31801 gaataaagaa aacaataaaa tggtacttag ataataaaga atggatggaa aatgtaacat
    31861 caggagatta tcaaaaatat tatgagaaga tgtatagata ataaattgaa tataggagat
    31921 aatatatgaa tttaagaaat aaatcaaaca caaagtatga actttttata tattttgtgc
    31981 tattctcaat atttattaga gactttaaaa tatatatacc tctatttgta ctaaatatat
    32041 tatatgttat atatcttatt tttaaaaaag agatacatat aaatttaaaa aaatggaata
    32101 ttactttagc tttatttata atatggacag ttataaatac tataatagca agttttattt
    32161 ttaaatatcc tgtatattat tcaaatgtta taaaactttt gcttaatatg agttttctat
    32221 tatctacata ttttgttgta gatggagcaa atatgaagtt ccataagaaa actttagtta
    32281 gatttttaga gtttataata ttgattaatt ttatacaaat tatatatatt tattttgatg
    32341 gcaagttatt taatgatttt tttagtggcg cactaacaga aagttctgat gcagcttata
    32401 caatcagtag ttatcataat ataattggag cagaaaataa gaatatttgg gctactaagt
    32461 ttgcattaat atatatgata tatctttaca tatgtgcatt cgataatttt aatataaatt
    32521 taattgaaaa aataagtttt ataattttag gtatagtaac agttgtactt ttattaagca
    32581 gaactgctca agtagctata ataataccga tagtattttt agctttttat agtatgagaa
    32641 atttaaatta taaatataga ataggtatat atgcagtaag tggaatttta ttcttgggag
    32701 tattgtttat cttttttgat aaatttttcc atttaaaatt taatatgaca gatggtggat
    32761 ttacaagatt atatatatgg aaagaattta ttataagtgt ttttgacact cattttatgg
    32821 taggtaatgg tataggatat agtgcttatt ttattcagga tatattagga aggtttgaat
    32881 caaatcttca taacgtattt cttaatatat tttttgaact tggaataata ggaattgttc
    32941 tttatattac ctttataatt caatttttta aagattttat tataagaaaa aatatattga
    33001 aaaatttatt tatgataata tttcctatgt tagccatagt ttgtttacaa tacttaggat
    33061 atgataatga tttagtagta actttaacac tattaattat aataaatatg attatgaaga
    33121 aagatgagca aagaatatga ttagaaaatt ttcattgata atggctacct atgggagaaa
    33181 agatgaagta gggtattttc ttgaaagtct aatgaattca aattatccaa tggataaagt
    33241 tgaagtaatc attgttgatc aaaataaaga aatagactta gagcccataa ttagtaaata
    33301 taaagaaaaa ataaaagtgg ttcacataaa atctaatgaa agaggattag ctttaaatag
    33361 aaacaagggg ttagagatag caactggaga tataatagct tttccagatg atgattgtga
    33421 atatctagta gataccctta atatagttaa taattacttt gaaaatgaac attgtgattt
    33481 attaatggga agaattgttg agcgtaatgg tgaagattca cttagggatt ggcctaaaga
    33541 gaagatagaa ataaataaaa ataactttta tacaaagtgt tcttcagtaa ctatgttttt
    33601 agatagtaaa aaatgctttg taaaatttaa tgatcaatta ggagcaggta aatattttgg
    33661 agcttgtgaa gatgcagatt tgatatataa aaactgtaaa aggggtctaa aaataatata
    33721 taatccagaa cttcaaatat atcatccaca ttatgattca aatagcaata tggatgataa
    33781 gaaaatatat agttatggtt taggttttgg tgcaatggta aaatcaaatt ttgatttttc
    33841 tatgctaata ttatttatta aggctgaggg atatcatttc ttaaaatcat taatatattt
    33901 aataacattt aagacaacta agtcaaaaag aagttggctt gcttttaaaa gtagaataaa
    33961 aggatttatt gaatataaga attaaccata aagcataaaa ggattttgaa atataaaatt
    34021 cttttatgca tttataatat aatttgctta tgaaataaga gatttttagg agatagttat
    34081 gagtaaagtg aaaagaaaat taaagaacat agtaagaaat attatgttga gtgaaacttt
    34141 tgcaaatacc aatttagtaa aaaatatgag aaaaaatcat aaggagaaat tagaagaagg
    34201 aagaagagtt ttatttaaag agcatgctaa agattgttta gaaactataa aggaaacttt
    34261 agataaaaat aatcttcatt tttggcttga ttatggaact ttgcttggag ctatgagaga
    34321 aaaggatttt atagcacatg atttagatat agatttagga atgttttacg aaaatcaagt
    34381 tgaagaagta gaaaaagctt ttaaagaagc taatataaaa aaagttcgtg aatttacttt
    34441 agatggtaag gtagtagagc aaacatatag ctataaagga ttatactttg atatatttta
    34501 ctattttaaa gatgaaaatc ttatgtggac atatggattt acttttaaaa ataataaact
    34561 aaataaagtt agttataaag ataaagatat atcaactggg tttgaaggaa tgaggtattt
    34621 tgttaagaag agaggccttg aaaaaatcat gtttaaagga aaagaatata cggttcctga
    34681 aaatccagat ggttatttaa gagaaaatta tggaccaaat tatatgacac ctataaagga
    34741 atgggattat gtagaagcac caacaaatca agaaaagtta aatggtggag acatagtaat
    34801 gatagaatat tataattagg aggaactatg aataaatcaa tatcgaaaaa tataatattt
    34861 aaattcttat taaatatttt taatgtagta gttccaataa ttataggtcc ttatgtatta
    34921 agagttttag ggccagactt aatgggaaca ataaacttct cccaaacaat atatggatac
    34981 tttttcatat ttgcaggttt tggagtatat caatatggtt taagagaaat aagtagagtt
    35041 agagacgata aaaaaaagct ctcacaggta tataccagtc tatttactct aacctttata
    35101 acaaatatac ttacaacaat tgggtatgta ttatttgttc agtttaatta ttctggaaca
    35161 gaaatatatg ttgcatgtat gatattaact tttaatttat tagctaatat cttttatact
    35221 gaatgggtaa atgaagggtt agaaaactat gacttcataa caataaaaac tatcataatt
    35281 agaattgttt atgtagtatt tttatttata cttgttagaa gtgcgaatga cttaaaagaa
    35341 tatatgattc ttttagtttt atcaacattt ttaaataata ttgtaagttt tatatacata
    35401 agaaaaagaa taccttttga tttttcagac ttaaaattat taagacatat aaaacctatg
    35461 tttttagttg ttatattatc aaatgccaat gtattataca ctcaactaga tagatatttt
    35521 ataggagaat atataaatat ggattctgtt gcttattata caacagcgca aaatataagt
    35581 aacataataa ataccttgtt gcttacagtt atttatgcaa ctattccaag aatgtcaaat
    35641 tatatagcaa atgaaaatca tgatgagtat aaaggtttat tagataaaat ttctagaatg
    35701 tattttatag ttcttttccc agctgctata ggtatgctag ttttagctaa agaggtaatt
    35761 cttatatatg gtggtagtaa gtatttagat gctataccaa tgcttaaggt ttttgctata
    35821 tatataatta ccttaggatt tgagaccata ttaagtaatc aggttatgta tataagagga
    35881 aaagaaaaag aacaagtaaa gataacattt atagggggag cagtaaacct agtattaaac
    35941 gtaacattat tatggacagg ctatttcaat ggaacaaatg cagtaataac aacaatgtta
    36001 gctaatatag tggttattat attagaatac ttatatataa aatttgttat ggacttagat
    36061 tttaacattt ttagcctaga taagatgaag tatctagcaa tatcattatt atttatacct
    36121 ataacttatt ttataggaaa atttataagt ggagttatac tatttacttt agcagtaatg
    36181 actataaact cattagttta tttcttgatt ttactagtta ttaaagatga ttgtttatta
    36241 gaaataataa ataaatttac taaaaaatta agaagataaa aataaaaggg ggagtttcat
    36301 tgattaaaaa gtttattatt gctacagtta ttactttgag tgtaacttca attaatgtta
    36361 tagctttaga gaatgtaaac aataattctg atgaaaataa atattctact ttagttggag
    36421 aaacttatga aattgtaaaa aaaccaaata tgtttttctt aatcccaaat gataatgtag
    36481 aaagttataa agatgaaaat ggaattaaaa gagaagaatt taaaaaggat aaagaaggtc
    36541 aagaatctat aaacagactt gttacaaaga ctaagagtaa gtatgagata gcattagctc
    36601 atgaaaatgg gaaatatact tttttagatt ctgcaaacac aaaggaagaa gcagagaata
    36661 aagttaaaaa tttaagtgga aaatataata cttttgcagc aatgcctgtt gttttaaatg
    36721 atagtggaca agtagcttat tcagaaaaat caatgggaag attagttaaa tataaaaatg
    36781 gaagtccagc tggatatgga gaaataacta atatatatgc taatcctaat ttaataaatg
    36841 actttacata tattaatcat ggttatgtag atgatgttcc aataattgag gatagaggaa
    36901 atgttgccaa aatagaagtt ggaggatatg aaggttgggt aaataaagat actagttctg
    36961 gaaattatga tttagtcata gtaccattaa accaagtaaa aaatccaagt tattatatag
    37021 ttagagatgg agagttaatc cattatataa gtagtgatct taccaattat tctgaaggtg
    37081 gatatgaggt tgttatagga ccagcaccta atttcttaaa tgaaaatgta aaatattata
    37141 gttatgataa caaatatttt tataaagatt taagtacatt aataggtgat ttacaaaatg
    37201 ataatcataa taattctgta aatgcaaata atccttttta tccatattat atgtatttac
    37261 catttagaag taaaacaaca tttactgctg aagaattaaa taattttatt gataaaaaga
    37321 caaaatcata tagtaaatta agaggaactg gacaagcttt tatagatgca caggataaat
    37381 atggagcaaa tgctctttta cttttaggac tagcggctaa tgaatcagct tggggaacat
    37441 ctcaaatagc tcaacaaaaa aataatttat ttggaataaa tgctattgat tcaagtccag
    37501 gagcatcagc aaattcattt gaaactgttg aaggctgtat aaatgatttt gcaaaatatt
    37561 atatttctag aggatattca gatccagaag attggagata ttttggagga taccttggaa
    37621 ataagggtag tggagctaat gttaaatatg cttcagatcc attctgggga gaaaaagcag
    37681 gacaaaatgc ttatatagct gattactgga caagtggaaa aggaatagca ggtttaaaag
    37741 attataatta ctatcagcta ggcatttata caggggctag tagtgttaca aataaagata
    37801 atgaaaaatt atatgatgta ggtagcttat atactgaaag agtagaaaaa ataggagcta
    37861 caacaatttt aactagcaaa gaaaagatta atcacaatgg aaaagaatgt tatgaaataa
    37921 atccagttag aacaactcct gtaatatcaa atggatcacc agtagcattc ccaggtccat
    37981 acgattggaa tgataaaggc tttgtagatg catcaaaagt taagcttata aatgaaggta
    38041 agtattctga aaatgttatg ggaaaatggg aattaaataa tggcatatgg tactattata
    38101 tagacggaaa atatgttact gggtggaata atatagataa taacaagtat tattttgatc
    38161 aatcaggtaa gatgcaaaca ggttggctat gtatagataa tatatggtat cattttagag
    38221 ataatggaac aatagacata ggttggagag aaatagatgg atattggtat tattttaata
    38281 atgatggaga aatgcaaaag ggttggcaga caataggtaa atataagtat tatttcaatg
    38341 ataatggagt aatgaatatt ggctggagaa aaatagataa tatttggtac tattttaata
    38401 taaatggaga aatgcaaaca ggatggttat ttgaaaataa tatatggtat cattttgaaa
    38461 ctacaggttc aatgactgta ggatggaaaa aaataaataa tgattggtac tattttaatt
    38521 caaatggaga aatgcaaaca ggatggcaaa atattggtac tccaaaatat cattttagag
    38581 ataatggtgt aatggacatt ggatggagag aaatagatgg acaatggcat tattttaatg
    38641 agaatggaga aatgcaaaca ggttggcaat atataaatgg aaatagttat tatttcgatg
    38701 aaaaaggaat atggaaacag taataaaaag actgattaaa ttcagtcttt ttattataga
    38761 ataaatttct aatattgata aatatagatc gataaggtaa tatttaagta taagcaagtt
    38821 tatacgctaa ttcaaaaaaa gaggtggaca agtgaagaga aaaagtattg cattagttct
    38881 ttctttagga ctgttaggtt ctgttacttt aggtagcatt gaaacagttt atgctgaagg
    38941 aaataagaac acaactaata ttagtgaagt aaaagttgac aataaggtag aattaagtga
    39001 caagaaaaac aaagagataa aaaatggatg ggaaattata aatgataagt ggtattatca
    39061 tgaaagtgat ggaagtttag ctactgggtt taaaaaaata aatgatgaat ggtatcgttt
    39121 tgataataat ggagaaatgc aagttggctg gcaatatata aatgatatat ggtaccattt
    39181 tagagataat ggacctatgg atactggttg gagaaagatt aataatgatt ggtattattt
    39241 taataatgat ggagaaatgc aaacaggatg gcagtctata ggaaataaaa gatatcattt
    39301 cagagataat ggagtaatgg acactggttg gagatcatta ggaagtgatt ggtatctatt
    39361 aaactcagat ggagaaatgc agacaggttg gcaacacata aataatatat ggtatcatct
    39421 tgaagatgac ggtaaaatga atactggatg gcaagatata aatggagaca aatattattt
    39481 taataatagc ggcgaaatgc aaacaggttg gaaatatata aatgatatat ggtaccattt
    39541 aagagaaaat ggacctgcag atactggttg gagaaaaata aataatgact ggtattactt
    39601 tgataatata ggtaaaatgc agacaagctg gcaagatatt gataactcaa catattattt
    39661 caatgataat ggagttatga gtattggatg gagaactata gaaagtgaat ggtattattt
    39721 taataatagt ggagaaaaag aaagcaacaa agttatagat gattggtatt tagataaaaa
    39781 tggagtagga aataagattg taactgaagg tgtatatgga aagagtggaa agggtagaga
    39841 tttagaatat tatagaatag gacatggtaa aaaggtatta ttctcagtgt ttggagtaca
    39901 tggatatgaa gatgcttgga gtggtgattc acaagaactt aaaataatag ctgaaaagtc
    39961 agtaaataga ttaagaagtg aatataatac taaaggaata gatttaagtg aatggagtgt
    40021 atatattata ccttcagcta atccagatgg aagaattgat ggatggacaa acaatggacc
    40081 aggtagaact acagtaacaa ctaagaatga tataaataga tcattcccta caggattttc
    40141 tcctttttat agtgctagaa actatacagg accaacagca ttaggttctc cagaggcaaa
    40201 ggctttatat agtttcataa ataaaactat ggaaggagca actgaaaaag ttcttttaga
    40261 tgtacatgga tgggaaaaca aaacaatagg taatcctagc attgctagat attttgataa
    40321 tgaatttggc tttagaaata taaataaata tccaggtgga tttgtaataa cttatggaaa
    40381 tgctatagga gctaaatcag ttttagtaga atttccagtt ccttattcac atcaagatat
    40441 attaagaaga gatttttcag gtaaatttgc taatggatta gtaaatattc ttttaaataa
    40501 ctaaaaata
//
LOCUS       DAEVGI010000009        36089 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEVGI010000009 DAEVGI010000000
VERSION     DAEVGI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 36089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36089
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.009"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(501..3020)
                     /gene="gyrA"
                     /locus_tag="K8M77_000461"
     CDS             complement(501..3020)
                     /gene="gyrA"
                     /locus_tag="K8M77_000461"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBI7084989.1"
                     /translation="MSLNEGKVIPIDINKEMKKCYIDYAMSVIVGRALPDVRDGLKPV
                     HRRILYSMQELGLYPERGYRKCARIVGEVLGKYHPHGDSSVYDALVRMAQDFSLRYML
                     VDGHGNFGSVDGDSAAAMRYTEAKMNKIASEMLRDINKNTVDFTPNFDGEEKEPVVLP
                     SRYPNLLVNGSSGIAVGMATNIPPHNLGEVIDGTIALIDNPELTSLELMTYIKGPDFP
                     TAGIIMGKSGIRAAYETGKGRIVVRAKAEIEEENGRHKIIVTELPYQVNKAKLIEYIA
                     DLVKDKKITGISDLRDESDREGMRIVIELKRDANPNVTLNLLYKHTKMQDTFGVIMLA
                     LVDNQPQILNLKQVLVHYINFQKDVITRRTQFELNKAKERAHILEGLIIALDNIDEVI
                     NIIRSSKTSEIAKNTLIERFSLSEKQASAILEMRLRRLTGLERDKIENEYNELMKLID
                     YLNAILASEERLLEVIKEELLEIKAKYSDERRTQIEKQENEIDIEDLIQEEDVVITLT
                     HTGYIKRISSDVYSAQKRGGKGIQAMTTKEDDFVENVLVTSTHSNLLFFTTKGKVYKI
                     KAYEVPDAGRTAKGTNLVNILPLEADEKIQTVLSFKDVQEDGYLFMGTKLGIVKKTPL
                     KDFKNIRKNGLIALNLREGDELLRAKITYGDADIIFVTQDGNAIRFNETDVRPMGRTA
                     SGVKAISLREGDVAVCMDIAVEDEKLLVVSENGYGKRTNLDEYKVQNRGGIGLITYKV
                     SDKTGKVVGATVCKEDDELMLINNQGVAIRINVSDISVTSRSAMGVKLMRTLEDEAVV
                     TIAKISGDTNEEEEEMTLLDEPEDTNIIVEEVATEVKDEEN"
     gene            complement(3042..4955)
                     /gene="gyrB"
                     /locus_tag="K8M77_000462"
     CDS             complement(3042..4955)
                     /gene="gyrB"
                     /locus_tag="K8M77_000462"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBI7084990.1"
                     /translation="MEQNNQNYDASQIQVLEGLEAVRKRPGMYIGSTSSRGLHHLVYE
                     IVDNSIDEALAGYCKNIKVYIHKDNSITVVDDGRGMPVGIHPKMGKPTVEVIMTVLHA
                     GGKFGGGGYKVSGGLHGVGASVVNALSELCEVIVTRDGYVWKQSYKRGAVLTGLEKIG
                     EAEGSGTKVHFKPDHEIFEETEYDFEILSQRLRELAFLNKGINITLIDEREDEVKEEV
                     YHYEGGIKSFVSYLNRNKEVLHDEPIYVEGLKDGIAVEVSLQYHDGFNENIFTFANNI
                     DTVEGGTHLAGFKTALTRVMNDYGRRFGHLKESDKNLSGDDIREGLTAVVSVKISEPQ
                     FEGQTKTKLGNSEVRSAVDSISGEGISTFLEENPEVGKIIIEKALLASRAREAARRAR
                     ELTRKSVLERSSLPGKLADCSSKDPKECEIYIVEGDSAGGSAKQGRDRKFQAILPLRG
                     KIMNVEKQRLDKILGSDTIRSMVTAIGAGIGPEFDIEKIRYNRIIIMTDADVDGAHIR
                     TLLLTFFYRYMRDLVEQGHVYIAQPPLYKISKGKQERYAYSDAAADEIIEEFGGKDSS
                     VNVQRYKGLGEMNAGQLWETTMDPESRILLRADIEDAMVADEIFTILMGDKVEPRREF
                     IQKNAKNVSNLDI"
     gene            complement(5195..5455)
                     /locus_tag="K8M77_000463"
     CDS             complement(5195..5455)
                     /locus_tag="K8M77_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HBI7084991.1"
                     /translation="MFLHLGENVVVPVKDVIGIFDIQTAMYSSDTIQFLRMAEEDGFV
                     ERITDEKPKSFVIAEIDKMSKVYLSPISSSTLTKRTDINYNP"
     gene            complement(5469..6554)
                     /gene="recF"
                     /locus_tag="K8M77_000464"
     CDS             complement(5469..6554)
                     /gene="recF"
                     /locus_tag="K8M77_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBI7084992.1"
                     /translation="MYIKSLQLINYRNYENLSIKLCPNVNVFIGDNAQGKTNVIEAIY
                     YCGFAKSHRTNRDKELIEWNKDRAFIRLDVHKDRLDKIIDVNILKDGKKAISINSIKI
                     SKIGELIGTFNVVMFSPEDLKIVKDSPGIRRRFIDMELSQLNKRYYHNLVQYNKVLHE
                     RNLVLKNKNINEEMLDIYDIQLAQYGENIIKTRLKYIEQLNKYSKEIHKEITSGKEEI
                     EFKYISTVKDLDNIKDSMIKLLEQNRKKDIDKRATSIGPHRDDFNIYLNNIDAKIYGS
                     QGQQRTSVLTIKFASLKIIKEITGEYPVLLLDDVLSELDFNRKRYVLTSIKNIQTVIT
                     CTGIEDLTSYLDENSKVFRVINGRIQC"
     gene            complement(6610..6816)
                     /gene="yaaA"
                     /locus_tag="K8M77_000465"
     CDS             complement(6610..6816)
                     /gene="yaaA"
                     /locus_tag="K8M77_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBI7084993.1"
                     /translation="MQEVKINTEFIKLDSFLKWCGAVSLGSEAKMFIMDGEVLVNGEV
                     CTQRGKKLRVDDTVEFNGEIYKLI"
     gene            complement(6942..8042)
                     /locus_tag="K8M77_000466"
     CDS             complement(6942..8042)
                     /locus_tag="K8M77_000466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBI7084994.1"
                     /translation="MIFTCDKNKLQESIAIAQKAITGKSTMPILEGLYIEASENIVTI
                     IGSDKDVSIETKFEADVTEPGKLVVDAKIFGEIIRKLPNDKIKIETVSEEAIRITCQK
                     SVFDLLHMNAEDFPSLPKINENMIFSINQGILKNMIKGTSFAIAQDETRPILQGILFE
                     IKDSKLNLVALDGYRLAIRTENIDTNNTINAVIPGKTLNEVAKILEDSEEIVNITFTP
                     NHILFNMGDTKVISRLLEGEFIKYDALLPKEHKLQIEVDKQTLQNGIERASLMAKEGN
                     SNLVKFDITDENIVITSNSQLGKVREEVTIKLQGDEIQIAFNSKYLLDVLKNMEEDKI
                     ELRMTSSISPCIIRNKNNDLCEYLVLPVRIAR"
     gene            complement(8336..9709)
                     /gene="dnaA"
                     /locus_tag="K8M77_000467"
     CDS             complement(8336..9709)
                     /gene="dnaA"
                     /locus_tag="K8M77_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBI7084995.1"
                     /translation="MDAQLNNLWEQALNIIKGEISEISFNTWIKSCTPISISDNILKL
                     SVPNEFTKGILDTRYKDLLIQALKIVTSRKFKIEFYLESDLEEEKENEEKQKEEKKEN
                     TNDVDGSIVVSDEMSATLNPKYTFQSFVIGNSNRFAHAASLAVAESPAKAYNPLFIYG
                     GVGLGKTHLMHAIGHYILQENPKAKVVYVSSEKFTNELINAIKDDKNEEFRNKYRKVD
                     VLLIDDIQFIAGKERTQEEFFHTFNALHEENKQIILSSDRPPKEIPTLEDRLRSRFEW
                     GLIADIQPPDFETRMAILKKKADVEGLNVPNEVMVYIATKIKSNIRELEGALIRIIAY
                     SSLTNRDVSVDLASEALKDIISNKESAPVTVKTIQESVANYYNLRIEDLKSQRRTRNI
                     AYPRQIAMYLSRKLTDMSLPKIGEEFGGRDHTTVIHAYEKISENLKTDEGLQSMINDI
                     TKKLTQK"
     gene            10740..10874
                     /gene="rpmH"
                     /locus_tag="K8M77_000468"
     CDS             10740..10874
                     /gene="rpmH"
                     /locus_tag="K8M77_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBI7084996.1"
                     /translation="MFMTYQPKKRQRSKEHGFRKRMKTKSGRNVLKRRRQKGRKRLTA
                     "
     gene            10935..11312
                     /gene="rnpA"
                     /locus_tag="K8M77_000469"
     CDS             10935..11312
                     /gene="rnpA"
                     /locus_tag="K8M77_000469"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBI7084997.1"
                     /translation="MLLSLRKNNEFRTVYRRGKSYANDLLVLYVYPNRKNVTKDGERF
                     NKVGVSVSKKVGKSVVRSRVKRLILENYRLNSSELKEGYDFVFIARVAINGKDFKQVG
                     KAMNNLIKKAGLRDNEKIVHSND"
     gene            11284..11493
                     /gene="yidD"
                     /locus_tag="K8M77_000470"
     CDS             11284..11493
                     /gene="yidD"
                     /locus_tag="K8M77_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBI7084998.1"
                     /translation="MKKLFIVMIKFYRKYISPLKRPCCRFYPTCSQYALEAIQKYGAF
                     KGGFMSIGRILRCNPFCKGGYDPVK"
     gene            11527..12243
                     /gene="yidC"
                     /locus_tag="K8M77_000471"
     CDS             11527..12243
                     /gene="yidC"
                     /locus_tag="K8M77_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBI7084999.1"
                     /translation="MQSILKPITNLFTMIFQAIHGFVASLDIFGVGAGYVITIFLLTL
                     LVRLILLPLNIKQTRSQQKMQEIQPEIAKLQKKYKNNPEKAQQEMMKLYKENNVNPMS
                     GCLPLLIQMPILFALYYVFYGLGELKGVSFLWLGDLWAPDRTFILPILSAATTYLSSL
                     LMTKFTQSQAGGAPGGMNMNTMNIVMAGMMGVMSIQFPSMLVLYWVIGNLIQMVQTYF
                     IVVLPSKKRAKEREQYKDIK"
     gene            12285..12908
                     /locus_tag="K8M77_000472"
     CDS             12285..12908
                     /locus_tag="K8M77_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBI7085000.1"
                     /translation="MNTIEITGKTVEEALKSALEQLNTTEDKVEVTVIEEGSKGFFNL
                     IGAKPAKISVKVKRDRILEAKKFLSDILDNMNIKAEINIEESKDLVKINLTGPKMGAI
                     IGYRGETLDSLQYLTSLVVNKAKEDKYKRVVLDTENYRQKREETLKRLANKIAYKARK
                     SGKILKLEPMNPYERRVIHSELQNNDFVRTFSEGEEPYRRVVVELKK"
     gene            13249..14625
                     /gene="mnmE"
                     /locus_tag="K8M77_000473"
     CDS             13249..14625
                     /gene="mnmE"
                     /locus_tag="K8M77_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBI7085001.1"
                     /translation="MKEFDTIAAIATALGEGGIAIIRVSGNKALEIVNKIFRGINGKD
                     LLDIKPYTMRYGHMIDENNEIIDEVIVSFMKGPRSFTAEDTVEINCHGGIVATNKVLQ
                     NVIKAGARLAEPGEFTKRAFLNGRIDLSQAEAVMDIITAKTELSMKSAMTQSQGRLST
                     EINNLRKEALDILALIEYAVDFTEDDEEPDETIQVKVKENVITLRGKVNNLIDTADEG
                     KLIRDGLSMVIVGKPNVGKSSLLNALLNEKRAIVTDIAGTTRDVIEEYINLDGIPVRL
                     VDTAGIRETEDVVEKIGVEKSKEKINEADLVILMLDTSRELDEEDKEIIDYIKDRKYI
                     VLLNKVDLDRKLSSEIVDNLENKIELSAKTGFGIDDLKSKIKDLFFNGSIDAESVMVT
                     NTRHKEALYRASENLDGALNGLNNNEFLDLVSIYVTSALRALGEITGAELEEDLVNKI
                     FAEFCCGK"
     gene            14640..16532
                     /gene="mnmG"
                     /locus_tag="K8M77_000474"
     CDS             14640..16532
                     /gene="mnmG"
                     /locus_tag="K8M77_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBI7085002.1"
                     /translation="MRYNAGSYDIVVIGAGHAGCEAGLAAARMGCKTLVCTLTLDSVA
                     MMPCNPNIGGTAKGHLVREIDALGGEMGVNIDHTFIQSKMLNTSKGPAVHSLRAQADK
                     KKYQERMKKVLETQENLHLRQLEVVSVEVEDGKIKGVLTKNGAFFECKAVIMTSGTYL
                     QSRIIIGDVSYSQGPNGLSNANELSKSLIDLGIDLRRFKTGTPARVNKRSVDFSKMIE
                     QPGDEEIIPFSFISGNIDRDQVSCWLTYTNEETHKVIQENIHRSPMYNGSIKGVGPRY
                     CPSIEDKVMRFQDKDRHQIFIEPEGDDTEEMYVGGMSSSLPEDVQVQMIKTVPGLENA
                     EIMRTAYAIEYDCIDPTQLKASLEFKNIDGFFSAGQINGSSGYEEAGAQGIVAGINAA
                     LKVQGKDPMILTRSDGYVGVLIDDLITKGTNEPYRMMTSRAEYRLLLRQDNADFRLTE
                     IGHNVGLVTEERWNKFQERKQNLERELERLKELQITNKTENNEKIVELGSTELKKPIR
                     MYELIKRPELDYFSLACLDPERPDLPKDIGDQINIIARYEGYIQTQLEQVAQFKKFEK
                     KVLPEDLDYNDVNSLRIEAIQKLNKIRPLNIGQASRISGVSPADISVLLIFLEHYRKT
                     GKNTDN"
     gene            16709..17428
                     /gene="rsmG"
                     /locus_tag="K8M77_000475"
     CDS             16709..17428
                     /gene="rsmG"
                     /locus_tag="K8M77_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBI7085003.1"
                     /translation="MQYFDLMKKACDSVGMEFNEDKYQKFMLYKDLLKEWNEKINLTA
                     ITEDEEIVKKHFIDCIKAFKADEFKKAKTVIDVGTGAGFPGLPIAIMREDVEVTLLDS
                     LNKRINFLNEVVNKLALKNVETIHSRAEDGARKKELRENFDIATSRAVANMCVLSEFC
                     IPYVKVNGNFIALKGPNITEELNDSKNAIGTLGGKLKGITEVEIEGTDLNHNLVIVDK
                     IKSTPKTFPRKAGNVTKKPLK"
     gene            17678..18451
                     /locus_tag="K8M77_000476"
     CDS             17678..18451
                     /locus_tag="K8M77_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBI7085004.1"
                     /translation="MKTICVFNQKGGVGKTTTNINICSYLAMQGFKVLAIDIDPQGNT
                     TSGLGIDKRKLDKSIYDLLTAGEEIETIIMKSELVENLYVVPATMELAGAEVEIIDRK
                     DREYILKNEINKIRDKFDYIFIDCPPSLGVLTINALVASHSVLIPIQAEYYALEGVGQ
                     LINTINLVKKSLNKDLDIEGVVMTMYDSRTNLSTEVYKEVKEYFKDRLYETTIPRNVR
                     LAEAPSFGLPICLYDERCRGAKSYEKLTEEFLKRQGVNI"
     gene            18452..19312
                     /locus_tag="K8M77_000477"
     CDS             18452..19312
                     /locus_tag="K8M77_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI7085005.1"
                     /translation="MAKKFGLGKGLGALIPEDNIKEEKKEKGGIINIELKDIKANKKQ
                     PRKFFDNNKLNELAESVKEHGIIQPLILKKEEKGYSIIAGERRYRAAKIAELTEVPAL
                     IMDISDEKLLQVSLIENIQREDLNPIEEGLAYERLLKEFALTQEELSKTMGKSRTTIT
                     NTMRLLKLDDRVKEYVMEGVISEGHGRAILPLENEAQYEIAQKIIDNSLSVRETEKLV
                     KKILNDAVEEKTSERRKPLNPYYKDVRDRLQDHFGTKVNLNANKNKGKIEIEYYSEED
                     LQRILDIINI"
     gene            19361..19858
                     /locus_tag="K8M77_000478"
     CDS             19361..19858
                     /locus_tag="K8M77_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HBI7085006.1"
                     /translation="MENVLNILTQYSTYITIGLIVLVLIQFILLIVALRSLSKVENKF
                     RKIMRGVNNKNLEELINSYLDKVEEVKKDSEETLETNKMLKAQVEKCTQKVSVIRYKA
                     FEDVGSDLSFSVALLDGENNGVILTGIYGRDYSTTYAKPIDKGISRYDLSEEELHVLN
                     AAMNK"
     gene            complement(20008..20598)
                     /gene="yyaC"
                     /locus_tag="K8M77_000479"
     CDS             complement(20008..20598)
                     /gene="yyaC"
                     /locus_tag="K8M77_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBI7085007.1"
                     /translation="MCTSFYINSLDSKASFKIRDYLCRELSPVIKENRPIIFICIGSD
                     RSTGDALGPLIGDKIKFLSKNNIFIYGTLEETVHAGNLKETVKIIKSKHKKPFIIAID
                     ACLGAVDNVGHVLIEKRPLKPGIALNKDLPEVGDLSILGIVNISGSLEFMVLQNTRLY
                     TVMLLANAISNGIHHCILKSVGFKKNILDSTLENIL"
     gene            complement(20648..21712)
                     /gene="ytvI"
                     /locus_tag="K8M77_000480"
     CDS             complement(20648..21712)
                     /gene="ytvI"
                     /locus_tag="K8M77_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBI7085008.1"
                     /translation="MKNLIIKSEKISVFFILYTLIFFTFFNTLKYTLPFLLAFIFSLL
                     LKTPTQFLIKKFKLNVGLASFISTFIFFLIIIILFTLFITSLAYESSSLTNYIQEIIN
                     TNSKEFQNYFISLQNWIEVNFIDNAVLNLIKNTLISSFKEITVLILSFGTELIQKVFL
                     LISYLPYTFMLLIFTVISTYFLSKELNTINFSKTLSNYLNSNYSNRILTILYEIKKMF
                     LTYCFSYLFLIFISFVLTFLGFSFLNIKYALLLSVIAALLDLLPVLGMPLIYFPLIFI
                     SYNSGNIFSAISLFILYIFIFLLRQFLEPKLMASTLGIHPLLILICIFIGLELNGLLG
                     IVFCIFLIIFFNIFKKVNVL"
     gene            complement(21724..22884)
                     /locus_tag="K8M77_000481"
     CDS             complement(21724..22884)
                     /locus_tag="K8M77_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBI7085009.1"
                     /translation="MNKIYFDNAATTFPKPDSVIKAMFDYMSFEGGSANRGSSSTALQ
                     SSRAVYECRYEIAKFFNFPKSENVIFTNNITTSLNMLLLGIIKSDWHIITTSMEHNSV
                     LRPLVKISEELPNVELDIVQCNNEGLVSVEKIKEKIKNNTKLIILSHASNLVGTIQPI
                     KEIGKLCKENDIFFILDSAQTAGVIPIDMTELNLNALAFTGHKSLLGPQGIGGFIIDD
                     KLNSICKNIFSGGTGSNSSLIEHPQELPDKFEYGTLNTPGIIGLLEGIKFIEKEGIEN
                     IKAKEEVLCQKAMDLLCEIPEVKIYGPMDAKKKTSTISFNIEGMDPEFTGFLLDSEFN
                     ITCRTGIHCTPLAHKTVGSYPAGSIRISLGYFNTIEEVYRFVEVIKELISRR"
     gene            complement(22896..23543)
                     /locus_tag="K8M77_000482"
     CDS             complement(22896..23543)
                     /locus_tag="K8M77_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBI7085010.1"
                     /translation="MSFFYLKAILSGIVTGLLVSIPLGPAAIEAATQSLDKGFRKGFE
                     VSLGAIAADYLYILLINFGVAQLLNLNRYVECAFWIISGLVLIIVNLIFKSSQKSLDE
                     KCIETIDSHKTGNGFINGFLITFVNPMTLSVWMAVSAGIMSIWKLSGRHFFLVALSAM
                     FITTLAWFILLNFMAAKGGSLVKKELTGKADKSVKYFMILLGLGFTIYGIFKIFM"
     gene            23701..23934
                     /locus_tag="K8M77_000483"
     CDS             23701..23934
                     /locus_tag="K8M77_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBI7085011.1"
                     /translation="MKDFIMVFNNTHEAMEGERILKENGIAFIIMPTPTYITKSCGIS
                     SAFNKDQLEKIKTLEIKFKNIYSITNKGFEIYK"
     gene            23967..24827
                     /locus_tag="K8M77_000484"
     CDS             23967..24827
                     /locus_tag="K8M77_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBI7085012.1"
                     /translation="MLLSSLLGINFEFDHINLGKISISLSTIDFLLKKFITICIIVMF
                     MTLVINIGNRLIDKAVQKQLDTNFKFSLDKGKAYTLGTLLKSILMYSVYFIGITTILS
                     IIFGNISWAFASVGGVAVGLGAQSFVKDVINGIFILFDNQYNVGDYVTIEGVSGIVEV
                     IGLRTTELRDQDGSLHIIPNGMIRIVTNNCRGDMRVQIDIGISYSDDINKVINTINSV
                     CDIYNKENNDITEPLKVWGVTDLTSSSVNLRICGKVKSMKLWETEVELRKRIKMTLDK
                     KKYMKEIITG"
     gene            24864..25076
                     /locus_tag="K8M77_000485"
     CDS             24864..25076
                     /locus_tag="K8M77_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBI7085013.1"
                     /translation="MINKFYVGDIVKMRKVHPCGSDEWEVIRVGADIKIKCLGCSRIV
                     MIPRSKFQKGVKKIIKSNAPEEEINE"
     gene            25220..25507
                     /locus_tag="K8M77_000486"
     CDS             25220..25507
                     /locus_tag="K8M77_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBI7085014.1"
                     /translation="MRKYETIFVAHPSLDEEAVKALIEKFKGVIENGNGTVDNVDFWG
                     KRKLAYEIAKVNEGYYTLINFTANPELPKELDRVFGITDGIIRHIIVKEEQ"
     gene            25521..25973
                     /gene="ssb"
                     /locus_tag="K8M77_000487"
     CDS             25521..25973
                     /gene="ssb"
                     /locus_tag="K8M77_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI7085015.1"
                     /translation="MNKVVLIGRLTKDPELRFAAGSGTAVARFTLAVNRQFKKDEADF
                     ISCIAFGKTGETIAQYITKGRQLAVSGNIRTGSYEAQDGTRRYTTDVVVESFDFIDSG
                     NGGARGNSGFGSGNDFGGSFGMPDNSFSDSSFNSNDDMTPIDDGDIPF"
     gene            26004..26246
                     /locus_tag="K8M77_000488"
     CDS             26004..26246
                     /locus_tag="K8M77_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBI7085016.1"
                     /translation="MSNVKRGGKFRRARKKVCIFCVDKAESIDYKDVAKLKKYITERG
                     KILPRRISGTCAKHQRQLTDAIKRSRNIALLPFTTE"
     gene            26529..26846
                     /locus_tag="K8M77_000489"
     CDS             26529..26846
                     /locus_tag="K8M77_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBI7085017.1"
                     /translation="MKKDNFNIMGDIKIIEEIKAQIICILGELFTLLTKGSNVAKDAI
                     VNCIASLIILLYILADKLGHSAIEVDETIKKSLKIGIVEEDNLEKQGGNLTKLFNHLK
                     ERR"
     gene            26864..27859
                     /locus_tag="K8M77_000490"
     CDS             26864..27859
                     /locus_tag="K8M77_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBI7085018.1"
                     /translation="MEEKNKSKSIVEAGIMSAITFVLMMLTSLPGIGTIISFAIPIPI
                     ALLYLKYNLKTSICSIVISGILIGFFMGSIYGIQLAITNGIIGLVLGTCVRKKFSGIK
                     SFIYLIVANTIGVLIEIALLFSVFTGITFNQYINDLVNVFNEAAKISEQMIGSSQANT
                     QMIELMKSMDASFIINMIPMVLVVVIIMRAFLSYTVGKYIINKLGFKLNSLPPFSKWF
                     FNPILIAAVVLINLFVMLLVSKGVINNKGMYMLTLNLLFAMLIIQGLAVVSNLLKYRY
                     RFSNFLIVFISILMVTSIPQLFGILGLVDVLIDIRGVDPNSLGSYIKEKLKRKLQ"
     gene            27892..29850
                     /locus_tag="K8M77_000491"
     CDS             27892..29850
                     /locus_tag="K8M77_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBI7085019.1"
                     /translation="MKSILEDYNKFLAYFAFVVLACLVFWKLNFKIVSLVIAFVYIIY
                     LIYKISYFLREEKDEEEKIDKLYKNVDEKVKDNIFNLIFPLTILNSTGEIIWYNHKFS
                     KLINSEDLLETNLVSVIKGVALDKIIRTGKENSQNIKIKSNIYEVYSEGLKLDESDKK
                     YFLVFFNDVTYLKKATKESIMLLEVDNFSEVLKTIDDDKKPLLIAEIERTINNYGNSL
                     NAMIKKYDNSKYILSVDDAIIGKEISKKFDILDTIREINLGNEIEVTLSIGVGRGGET
                     PAQNQDDATKAKELALGRGGDQAVIKSGKNIEFFGGNAKELEKRTRVRARVVAHVLKE
                     LVYESSTVYIMGHKNPDMDCFGASFGLASVVKKLGKNVNIVLEKDTNAIEFFLDRVKE
                     KEEYSKLFISTEEAKKNIDDNTLLIIVDVNSANYVLDRELTELIERVVVIDHHRRSPK
                     DIKGALLNYIEVYASSTSELVTEMVQYMLDKPKLNRIEAEGLLAGIYIDTKNFSFKTG
                     VRTFEAASFLRRLGADTVEIKKIFSNNLENYIAKAEIIKSAKVENKIAIAVCPENITD
                     TVVAAQAADELLNITGIQASFVLIKLGEDVAISARSLGDINVQVILEELGGGGHMTMA
                     GAKIKDTTIDEAIEKIKEAIAKNSREGE"
     gene            29852..30298
                     /locus_tag="K8M77_000492"
     CDS             29852..30298
                     /locus_tag="K8M77_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBI7085020.1"
                     /translation="MKVILLQDVKKLGKKGDVINASDGYARNFLFPKKLAQEATDNNL
                     HILNNKKENERRQKLAELEAAQALAADLKDKVIKIDGKAGDNGKLFGAITSKDVAGLI
                     KKQFNVEVDKKKIVMDTIKIAGTYNIEVKLYPEVSTKMKVMVVPVQ"
     gene            30314..32212
                     /gene="lonC"
                     /locus_tag="K8M77_000493"
     CDS             30314..32212
                     /gene="lonC"
                     /locus_tag="K8M77_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HBI7085021.1"
                     /translation="MKSIKELEVLQDVMNSELGVESQVEALKDIINNILDEGAFRARV
                     IRFKVQNYINSTDPYERLYGLSKIVSEGKGLSEVPTEETINEALEDVCAMISDAIARR
                     YVQNKIEKEVEQFLMEKQEKYVDELRVNIMKKKKGPENTKTEKKLEELEELDERVPNK
                     NIMSLLRPDSFDEVVGQERAVKSLLSKLASPYPQHIILYGPPGVGKTTAARIALETAK
                     KLKSTPFDDRSKFIEVNGTTLRWDPREITNPLLGSVHDPIYQGSKRDLAEIGVPEPKP
                     GLVTEAHGGILFIDEIGELDEILQNKLLKVLEDKRVEFSSSYYDPDDENTPKYIKYLF
                     DKGAPADFVLIGATTREPGEINPALRSRCTEVYFEPLSSRDIEKIVLNAAKKLNVKLE
                     EGLEKKIASYTIEGRRAVNILADAYGHAIYGLEGEVPEDLEITSKDLNEVVSIGRFTP
                     YEILENLEEKEVGHVYGLGVSGFLGSTIEIEATAFKAKKKGAGKIRFNDTAGSMAKDS
                     VFNAASVIKRLTDKDINDYDIHVNVIGGGKIDGPSAGAAITICIMSALLEKPIRQDLA
                     ITGEISLRGKIKPVGGIFEKIYGARRKGIKLVTVPKDNENEIPKGLEDIEVKAISSIE
                     ELMEIAFN"
     gene            32631..33965
                     /locus_tag="K8M77_000494"
     CDS             32631..33965
                     /locus_tag="K8M77_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI7085022.1"
                     /translation="MEAPVMKSLPQSIEAEQSVLGAMIIDRTTIAQAAEVLNKDSFYR
                     DAHKVIFQSIIEMYQRDQAVDQLTLLEYLKSTDRLEKAGGISYVTEISASVLSTVNIK
                     SYIKIVDEKAMLRRLIKSSTEIIEDCYNNQEDVEKVIDSAEKKIFDLSEKRSSNDFEP
                     LSVVLERGFEQIEAIFNNEGELTGVGSGFKDLDEMTSGFQKGDMVLIAARPSMGKTTF
                     ALNIAEHAALREGKSVVIFSLEMSKEQLAYKLLCSEANVDMLSLRTGKLTPEDWDNIA
                     RASGPLASAKIYIDDTAGVSVMEMRSKCRRLKMEYGIDLILIDYLQLMSGSGESRQQE
                     VAEISRNIKAIAKEMECPVIALSQLSRAPEQRADHRPMLSDLRESGSIEQDADLVMFL
                     YRDEYYNKETEDKNVGECIIAKQRNGPVGTVKLAWLGQYSKFADYEAIHKDV"
     gene            34144..34944
                     /gene="proC"
                     /locus_tag="K8M77_000495"
     CDS             34144..34944
                     /gene="proC"
                     /locus_tag="K8M77_000495"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBI7085023.1"
                     /translation="MNKKIGFIGCGNMGSAMVLGILKSGIVCKENIIASCSRESSAER
                     IKNELGINTTLDNKEVCNNSDIIFLAVKPYMYEKVIKEIRDCVDLNKTIVTIAAGINL
                     SDVEKWFKKETKIVKTMPNTPALVGEAMSAICPNELVNEEELKEVMDIFNAFGKSEVL
                     SEKDFHGFIALCGSSPAYVYMFIEAMADAAVREGISRDKAYKLAAQSVLGSAKMVLET
                     GEHPAKLKDNVCSPGGTTIEAVLELEETGFRSSVMSAMKKCSKKSKKM"
     gene            35280..36005
                     /locus_tag="K8M77_000496"
     CDS             35280..36005
                     /locus_tag="K8M77_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7085024.1"
                     /translation="MFKRSRGIALYHQLETELIDLINSGELKENDKLPSERELCEQYN
                     VSRTTARQAIGELERKEYVYKVHGKGTFISPKVYKQQLLKFYSFTEEMKKLGKNPSSK
                     ILSFDLIRADNKISEKLKVEENSLVYKIVRIRIADEVPMMVECTYLPEYRFIDLKEKM
                     LKEKPMYDIFREVYNVSLTKAKESFKPILISKSDSKLLNVEDGTAAMRIERVTFENER
                     VIEYTVSVSRGDKFEYTVVLEED"
BASE COUNT        13483 a         4588 c         5736 g        12282 t
ORIGIN      
        1 atgttaatat cttctgttta attttcaaag accatttctt cgtcgccctc aagcgacttc
       61 tttagtttaa catcactttc gaactttgtc aacatttttc ttcaaaaaac ttttttaact
      121 tatttactaa gttttgtttt ttgttttgtt gctgttcgtc agcgacaagt aatatcttat
      181 cacataagat tacataaaca tttagtttat atgctatatt ataaaattat attatatttt
      241 ctattaattt ctttattttt ctcaaaaatt taattattga tacacttggt aaagtttata
      301 ttatatttat actatttatt tgctattcat aaggattttt agatatttta atataataat
      361 ttgcctaaca aaactctttt taatctagtt aagttttatt tatattttat tatttaactt
      421 aaaaaatatg aattttaata gaaaaattta aaaaaataaa aactctgatg ctaaaagcac
      481 cagagtttca ttaaaagtta ttagttttct tcatccttaa cttcagtagc tacttcttct
      541 acaattatat ttgtatcttc tggttcatct aataaagtca tttcttcttc ctcttcatta
      601 gtatctccag agattttagc aatagttaca acagcttcat cttctagagt cctcataagt
      661 ttaactccca ttgcagatct acttgtaact gaaatatcac ttacatttat tcttatagct
      721 accccttggt tatttataag cataagctca tcatcttctt tacatacagt agcaccaact
      781 actttaccag tcttatcaga tactttatat gttataagac ctattccacc tctattttga
      841 actttatatt catctaggtt agttctctta ccataaccat tttcactgac aactagtaat
      901 ttttcgtctt ctacagctat atccatacaa acagcaacgt cgccttctct taagcttata
      961 gctttaactc cagatgcagt tctacccata ggtctaacat ctgtttcatt aaatcttata
     1021 gcattaccgt cttgagttac aaatataata tctgcatctc catatgttat tttagctctt
     1081 agaagctcgt caccttctct aaggtttaat gctattaatc cattctttct tatgttcttg
     1141 aagtctttta atggtgtttt cttaactatt cctagtttag ttcccataaa taagtatcca
     1201 tcttcttgaa catccttaaa tgaaagaaca gtttgtattt tttcatctgc ttctaatggt
     1261 aatatattta caagatttgt tcccttagca gttcttcctg cgtctggtac ttcataagct
     1321 tttatcttat aaaccttacc cttagttgta aagaataata agtttgagtg tgttgatgtt
     1381 acaagtacat tttcaacaaa gtcatcttct tttgttgtca ttgcttgtat tcccttacca
     1441 cctctctttt gagctgagta aacatctgaa gatattctct taatatatcc agtatgagta
     1501 agagttatta caacatcctc ttcttgtatt aagtcttcta tatctatttc attttcttgc
     1561 ttttctattt gtgttcttct ttcatctgag tacttagctt ttatttctaa aagctcttct
     1621 tttataacct ctaataatct ttcctcacta gctaagattg catttaagta atctattaac
     1681 ttcataagct cattatactc attttcaatc ttatctcttt caagaccagt aagtcttcta
     1741 agtctcattt ctaagattgc agaagcttgc ttttcactta aactaaatct ttcaataagt
     1801 gtgtttttag ctatttctga agtcttagaa cttcttatga tatttataac ctcatcaata
     1861 ttatcaaggg ctattattaa tccttctaat atgtgagctc tctctttagc tttattaagc
     1921 tcaaattgag ttcttctagt tataacatcc ttttggaagt ttatatagtg aactaaaact
     1981 tgttttaagt ttaatatttg tggttgatta tcaactaaag caagcattat aactccaaat
     2041 gtgtcttgca tttttgtatg tttatataaa agatttaatg taacatttgg atttgcatct
     2101 ctcttaagtt ctataactat tctcatacct tctctatcag attcatctct taaatctgat
     2161 attccagtaa tctttttgtc cttaacaaga tctgcaatat attctataag cttagcctta
     2221 ttaacttggt atggaagttc agttactatt attttatgtc ttccattttc ctcttcaatt
     2281 tcagctttag ctcttactac tattctacct tttccagttt cataagcagc tcttattcct
     2341 gatttaccca ttattattcc tgctgtaggg aaatctggtc ctttaatgta tgtcattaac
     2401 tctaaacttg ttaattctgg attgtctata agagctatag ttccatcaat tacctcacct
     2461 aagttatgtg gcggtatatt agttgccata ccaacagcta tacctgatga tccattaact
     2521 aaaaggttag gatatcttga aggtaataca actggttctt tttcctcacc atcaaagttt
     2581 ggagtaaaat caactgtatt tttattaata tctctaagca tttcagaagc tattttattc
     2641 attttagctt ctgtatatct cattgccgct gctgagtctc catcaacaga cccaaagtta
     2701 ccatgtccgt caacaagcat atatcttaaa gagaaatcct gtgccattct aactaatgca
     2761 tcataaactg atgaatcacc atgtgggtgg tatttaccta aaacttcccc aactattctg
     2821 gcacattttc tatatcctct ttcaggatat agtcctaatt cttgcattga atataaaatt
     2881 cttctgtgaa ctggttttaa accatctcta acatctggta atgctctacc aacaataacg
     2941 ctcatagcat aatctatgta gcactttttc atttctttat ttatatctat gggtattact
     3001 tttccctcat ttaagctcat gtacttcacc tcatattagt actatatatc taaattgcta
     3061 acatttttag cattcttctg aataaattct cttcttggct ctactttatc gcccataaga
     3121 attgtaaata tctcatcagc taccatagca tcctcaatat ctgctcttaa taatattctt
     3181 gattctggat ccatagtagt ttcccaaagt tgtcctgcgt tcatttctcc aagacctttg
     3241 tatctttgaa catttacact actatccttt ccaccaaact cttctattat ttcatctgca
     3301 gcagcatctg agtatgcata tctctcttgt tttcctttag aaattttata tagtggtggt
     3361 tgcgctatat aaacgtgtcc ttgctcaact aagtctctca tatacctata gaagaatgtt
     3421 aataatagag ttcttatgtg agcaccatca acgtcagcat cagtcatgat tattattcta
     3481 ttatatctta ttttttctat atcaaactca ggtcctatac cagctccaat tgcagttacc
     3541 attgatctta tagtatctga acctaagatt ttatccaatc tttgcttttc tacgttcatt
     3601 attttacctc ttaaaggtaa tatagcttgg aattttctgt ctcttccttg ctttgcagat
     3661 ccaccagctg agtctccctc aactatgtaa atttcacatt cctttggatc ttttgatgaa
     3721 caatcagcaa gcttacctgg taatgaactt ctttcaagta ctgattttct tgtaagttct
     3781 ctagctcttc ttgcagcttc tctagctctt gatgctaata aagctttttc tattataatt
     3841 ttaccaacct caggattttc ttctaagaaa gtagaaattc cttctcctga aattgaatca
     3901 acagcacttc taacttcact atttcctaat ttagttttag tttgaccttc aaattgaggc
     3961 tcagatattt taacagaaac aacagcagtt aaaccttctc ttatgtcatc tcctgataag
     4021 ttcttatctg attcctttaa gtgaccaaat cttctaccat aatcattcat tactcttgtt
     4081 aaagcagttt taaatcctgc taagtgagta ccaccttcaa cagtgtctat attattagca
     4141 aaagtaaaga tattttcatt gaaaccatca tggtattgta aagaaacctc tacagcaatt
     4201 ccatctttaa gaccttctac ataaattggt tcatcatgta aaacctcttt atttctattt
     4261 agataactta caaaagattt tataccacct tcatagtgat atacttcttc tttaacttca
     4321 tcttctcttt catcaataag agttatgttt attcccttat ttaagaaagc taattctcta
     4381 agtctttgtg ataaaatttc aaaatcatat tcagtttctt cgaatatttc atggtcaggc
     4441 ttaaagtgaa ctttagttcc actaccttca gcttctccta ttttctctaa acctgtaagt
     4501 acagcccctc ttttataaga ctgtttccaa acatatccat ctcttgttac aattacttcg
     4561 cataattctg aaagagcatt aactacagat gcaccaacac catgtaatcc accagatact
     4621 ttgtatccgc ctcctccgaa tttacctcca gcatgtagaa ctgtcattat aacttctact
     4681 gttggttttc ccatctttgg atgaattcct actggcatac ctcttccgtc atcaactaca
     4741 gtaattgaat tatccttgtg tatgtatact tttatatttt tacagtatcc agctaatgct
     4801 tcatcaatac tgttatcaac aatttcgtaa actaaatgat gaagacctct tgaactagta
     4861 ctaccaatat acattcctgg tctttttctt acagcttcta gtccctctaa gacttgaatc
     4921 tgacttgcat cataattttg attgttttgc tccaacatat aactcctcct atatagcagc
     4981 aatttacctt ttatagggtc ttccgtgaat aatctcaaac aacttagtcg aaattccttc
     5041 aatcctcaca cttaatgtaa acattattta agagctatta atttaaaggc tatgggttaa
     5101 cccttcccaa aataaatgtg gcaatcctat ttttataaaa tcttttaaaa ataaatcttt
     5161 agtaataacc tcaaatttat cttaattcaa aaatttaagg attataatta atatctgtac
     5221 gctttgttaa tgtagatgat gaaataggtg aaagatatac cttactcatt ttatctatct
     5281 cagctataac aaaagatttt ggcttttcat ctgtaattct ttcaacaaag ccatcctctt
     5341 cagccattct taagaattgt atggtatctg aactatacat agcagtttgt atatcgaata
     5401 ttcctataac atctttaact ggaactacta cattctctcc taagtgtaaa aacacaaata
     5461 ctcctccttt aacattgaat acgtccattt ataactctaa atacttttga gttttcatct
     5521 aaataactgg ttagatcttc tattcccgta catgttatta ctgtttgtat attttttatt
     5581 gatgttaaaa catatctttt tctattaaag tctaattctg acaatacatc atctaataat
     5641 aacacaggat attcacctgt tatttctttt attattttta aagatgcaaa ttttattgtt
     5701 aatacactag ttctttgctg accttgtgaa ccataaatct ttgcatctat attatttaaa
     5761 tatatattaa aatcatctct atgtggacct atagatgtag ctcttttatc tatatccttt
     5821 tttctatttt gttctaatag ttttatcata gaatctttaa tattatctaa atcttttaca
     5881 gtggaaatat atttaaattc aatttcttct tttcctgaag ttatctcttt atgaatttct
     5941 ttagaatatt tgtttaactg ttctatatat tttaatctag tctttataat attctctcca
     6001 tattgagcca attgtatatc atatatgtct aacatttcct catttatatt tttattcttt
     6061 aaaactaaat ttctttcatg taagacttta ttatattgaa ctaaattatg ataatacctt
     6121 ttgtttaatt ggctaagttc catatcaata aatcttcttc ttattccagg agaatctttt
     6181 actattttta aatcttctgg agagaacata actacgttaa atgttccaat taattctcca
     6241 attttgctta tctttattga atttatgcta atagcttttt ttccgtcttt taaaatattc
     6301 acatctatga ttttgtctaa tctatcctta tgaacatcta accttataaa agctctgtcc
     6361 ttattccact ctattagctc cttgtctcta ttagttctgt gtgacttagc aaatccacaa
     6421 taatatatag cttctattac attggtttta ccttgtgcat tatcacctat aaagacattt
     6481 acattaggac aaagcttaat tgataaattt tcataatttc tataattaat taattgcaaa
     6541 ctttttatat acataaaaac acctatattt aattatacca tattcttatg ttctttaggc
     6601 tatgtatctt taaattaact tataaatttc cccattgaac tctacagtat catcaactct
     6661 taattttttt cctctttgag tacatacttc accatttact aaaacttctc cgtccattat
     6721 gaacatttta gcttctgatc ctaagctaac tgctccacac cattttaaaa atgaatctaa
     6781 ctttataaac tctgtattaa tttttacttc ttgcataatt atacacacaa tgtatattta
     6841 aaaattacat tgtttctcct ttcaaaaaaa taaaataaaa aatagtctat ggtgagttta
     6901 gcaatactaa actcaccata gttataaaaa tttaatttaa attatcttgc tattctaaca
     6961 ggtagaacta aatattcaca taaatcattg ttcttatttc ttataataca tggagatatg
     7021 ctacttgtca ttcttaattc tattttgtcc tcttccatat tttttaaaac atctagtaag
     7081 tattttgagt taaatgcaat ttgaatttca tctccttgca atttgatagt aacctcttct
     7141 ctaacttttc ctaattgaga attagatgtg attactatat tttcatcagt tatatcaaac
     7201 ttaactaagt tactatttcc ttccttagcc ataagagatg ctctttcaat accattttga
     7261 agagtttgtt tatcaacttc aatttgtaac ttatgttctt ttggtaataa ggcatcatac
     7321 tttatgaatt caccttccag caatctagaa ataacttttg tatctcccat attaaataat
     7381 atgtgatttg gagtaaatgt tatgttgact atttcctctg aatcttctaa tatcttagcc
     7441 acttcgttaa gtgtctttcc tggtataaca gcattaattg tattgttagt atctatattc
     7501 tctgttctta ttgctaatct atatccatct aaagctacta agtttaattt agagtcctta
     7561 atttcgaata atataccttg aagaataggt ctagtttcat cttgagctat ggcaaaagaa
     7621 gttcctttta tcatattttt aagaattcct tggtttattg agaatatcat attctcgttt
     7681 atcttaggta agcttggaaa atcttctgca ttcatatgta ataaatcaaa tacagatttt
     7741 tgacaagtta ttcttatagc ttcttctgat actgtttcta ttttaatttt atcgttaggt
     7801 aattttctta ttatctctcc aaatatttta gcatcaacta ctaactttcc tggttcagta
     7861 acatcagctt caaatttagt ttctatacta acatctttat ctgatcctat tatagttact
     7921 atgttctcag aagcttctat gtatagacct tctaagatag gcattgttga ttttccagtt
     7981 atagcttttt gagctatagc tatagattct tgtaatttat ttttgtcaca tgtaaatatc
     8041 aaaataaaac cctcctttta ggacttattc acataagtta tacacagtgt gaatttttgc
     8101 ataatagata tattaataga taataaatat aataataata gtaatagtac ctgtggattt
     8161 gtggataaaa cctggacacc tttgtatacc aaggcttaca tggatttttt gcttgtggat
     8221 aacctgtaga ttactgtgaa caattatcca caataaaata acaggcaaaa aataataaac
     8281 ataataattc acaggttatc cacagataat atacagttat tatgtacttt gttaattact
     8341 tttgagtaag ctttttagta atgtcattaa tcatgctttg tagaccttca tcagtcttta
     8401 aattttcaga aattttttca taagcatgaa ttactgtagt atgatctctt ccaccaaatt
     8461 cttcacctat tttaggtaaa gacatatctg taagttttct acttaaatac atagcaattt
     8521 gacgaggata agctatattt ctagttcttc tttgagattt taaatcctct attcttaaat
     8581 tataataatt tgctacggat tcttgaatag tttttactgt aactggagca ctttctttat
     8641 tagatattat atctttaagt gcttctgaag ctaaatcaac gcttacatcc ctgttagtta
     8701 gagaggaata agctattatt cttataagcg caccttctaa ttctcttata ttcgatttaa
     8761 ttttagtagc tatatatacc ataacttcat ttggtacatt taagccttca acatcagctt
     8821 ttttctttag tattgccatt ctagtttcga aatcaggtgg ttgaatatct gctattaaac
     8881 cccattcaaa tctagatctt aacctgtctt ctaatgtagg aatttcctta ggcggtctat
     8941 ctgatgatag tataatttgt ttattctctt catgtagagc attaaaggta tggaagaact
     9001 cttcttgagt acgttcttta cctgctatga attgaatatc atctattaat aaaacatcga
     9061 cttttctata tttgtttcta aactcttcat tcttatcatc tttaatagca ttaataagtt
     9121 cattagtgaa tttttcagat gatacataaa ctacttttgc tttaggattt tcttgaagaa
     9181 tataatgtcc tattgcatgc attaagtgag tttttccaag tcctactcct ccatagataa
     9241 ataatgggtt ataagctttt gctggtgatt ctgcaacggc taaagatgct gcatgagcaa
     9301 agcggttact attacctatt acaaaggatt ggaaagtata tttaggattt aatgtagctg
     9361 acatttcatc acttacaact atagatccat cgacatcatt tgtattctct ttcttctctt
     9421 ctttctgctt ttcctcattt tctttttctt cttctaaatc agactcaaga tagaactcta
     9481 ttttaaactt tctagatgta actatcttta aagcttgaat taataagtct ttatatcttg
     9541 tatctagaat ccctttagtg aattcattag gaacagaaag ttttaatata ttatctgaaa
     9601 ttgatatagg agtgcagctt ttaatccaag tattaaagct tatttcagaa atttctcctt
     9661 ttattatgtt caatgcttgt tcccagagat tatttagttg ggcatccatc ttctatcctc
     9721 caatcataaa atgattttaa cttttagtta tgcacatata attaacatat tagattatta
     9781 ctttacatgt tgtgaataaa attattaaca ggctgttgat aatgtggata aattttatta
     9841 taaaaaaatt atcaacaaga aatatacaca gttcttgttg aaagtttaaa atatataaaa
     9901 ccgttgaaat aaaaggatta taaagactat aaataaaaaa taataagaat aatatacaag
     9961 ttatcaacag gattattcac aagtgtggat aagttgttat attagataaa gttattcaca
    10021 atgcattaat atgataacat aaaggtatag ttggattcaa taagttatcc acaaaaataa
    10081 cagctgttga taacttattt tgtgtaaaac ttatttagat ttatagttaa gtgaaaatat
    10141 taaaatttaa aggtacatat taggaaaaat atcttttaaa atttgaattt ttaacataat
    10201 agaagttaag ataatcaata tttaaatata ataaggataa attagggtgt aaaaagaggg
    10261 agagataata taaaggtaaa aagttaataa catattgtta tttatatgtg gataacttta
    10321 taactaaagt tataaagtta agtttattta tgataaatga gctaatctta tctatatata
    10381 tgtaattgta tatctttaag atttaaataa actttttatt gaagtaatta gtattaatta
    10441 agtagatgag taatttaata aattgtggat aaaataatag atagttttat ttagaataca
    10501 gtttatatat tttttagagt tattaatgta gggatttaat ttaatgtata aacaattatt
    10561 atttatatat ttaaaaaagg aaaataaggt agatgtaaca aatctttaag tgagttattt
    10621 tcttgacaga gggcgacttg aaagatataa ttaaacagta aaacctaaat tagcgaataa
    10681 gtaaaaaagc ttttagcttt ttactagaat aaaatattgg ttgaaggggg tgtatttaaa
    10741 tgttcatgac atatcaacct aaaaaaagac aaagaagcaa agagcatggt ttcagaaaga
    10801 gaatgaaaac taaatctgga agaaatgttt taaagagaag aagacaaaaa ggtagaaaaa
    10861 gattaacagc ataagggccg cattttgtgg cctttttctg cactaaaaag cagaaaaaag
    10921 gagagcaaat ttaaatgtta ctaagtttaa gaaagaacaa tgagtttaga acagtttata
    10981 gaagaggaaa gtcttatgct aatgacctac tagttcttta tgtttatcct aacagaaaaa
    11041 atgttacaaa ggatggagaa cgtttcaata aggtaggagt ttcagttagc aagaaggttg
    11101 gaaaaagtgt agttagaagt agagttaaaa gacttatact tgaaaattac agacttaatt
    11161 ctagtgaact taaagaaggc tatgactttg tgtttattgc tagagttgct ataaacggta
    11221 aggattttaa gcaagtaggg aaagccatga ataatttaat aaaaaaggca ggtctacgtg
    11281 ataatgaaaa aattgttcat agtaatgatt aaattctata gaaaatatat ttcaccttta
    11341 aagaggcctt gttgcagatt ttatccaact tgttctcaat atgcactaga agcaatacaa
    11401 aagtatggtg cttttaaagg gggatttatg agtataggaa gaatattaag gtgtaatcca
    11461 ttctgtaagg gtggatatga tcctgtaaaa taaatatata cggtttaaat ataggagggt
    11521 aatgtattgc agtcaatttt aaagccgata actaatttat tcacaatgat atttcaagct
    11581 atccacggct ttgttgcctc actagatatt tttggtgtag gtgctggata cgtaataact
    11641 attttcttat taactttatt agtaagatta atattattac cgctaaatat aaagcaaact
    11701 agatctcaac aaaagatgca agaaatacag ccagaaatag ctaaacttca gaaaaaatat
    11761 aaaaataatc ctgaaaaagc tcaacaagaa atgatgaagc tttataagga gaataatgta
    11821 aacccaatga gtggttgttt acctttatta atccaaatgc caatattatt tgcattatac
    11881 tatgtattct atggattagg tgagttaaaa ggtgtatcat tcttatggtt aggagattta
    11941 tgggctccag atagaacatt catattacca attttatcag ctgcaacaac atatttatca
    12001 tctttattaa tgacaaagtt tactcagtca caagctgggg gagcacctgg tggaatgaat
    12061 atgaatacta tgaatatagt aatggctggt atgatgggtg taatgtcaat acaattccca
    12121 tcaatgcttg tattatactg ggtaataggt aacctaattc aaatggttca aacatacttc
    12181 atagttgttt tacctagcaa aaagagagct aaggaaagag aacaatataa agatattaag
    12241 taataataat ttattttgct tttctaaggt ggtgaaacaa gggtatgaat acaattgaga
    12301 ttactggaaa gactgttgaa gaagctttaa aaagcgcatt ggaacagttg aacactacag
    12361 aagataaagt agaggttact gttatagaag aaggaagtaa aggcttcttt aatctaattg
    12421 gtgcaaagcc tgctaaaatt tcagttaagg ttaaaagaga tagaatctta gaggctaaaa
    12481 agtttttaag tgatattctt gataacatga atataaaagc tgaaattaat attgaagaaa
    12541 gtaaagacct agtaaagata aatcttactg gacctaagat gggagccatc ataggataca
    12601 ggggggaaac tttagactca cttcaatatt taactagttt agttgttaat aaggctaagg
    12661 aagacaagta caaaagagtg gttttagaca ctgagaatta tagacagaaa agagaagaaa
    12721 ctcttaaaag attagctaat aaaatagctt ataaggcgag aaagtcaggt aagatattaa
    12781 aacttgaacc tatgaatcct tatgaaagaa gagttattca ttcagagtta cagaataatg
    12841 attttgttag aacttttagt gaaggtgaag aaccttacag aagagttgta gtagaattaa
    12901 agaaataaaa aggaaagctt aaagctttcc tttttttatc cacaataaac tgtagataaa
    12961 ttgtggataa cttgttagta attatagaaa agtagtcata ccaaaggcta caattgttga
    13021 taaatttaaa aaataaggtg aaaatattat atttccacct tattttttta tcaagtatat
    13081 gatttaatta attggatagt attttaagta agtaatctat taaacaattt agtaaaatca
    13141 tatttttttg ccataaataa tatgatttaa aattttgact tattctattt tgttgggtaa
    13201 aatcaatatt agttcaaatt tgagaatatc attataaagg aggagcttat gaaagaattc
    13261 gatactattg ctgctatagc tacagcatta ggagaaggtg gaatagctat aattagagta
    13321 tcaggaaata aagctcttga gattgtaaat aaaatattca gaggaataaa cggaaaagat
    13381 ttattagata taaaaccata tactatgaga tatggtcata tgatagatga aaataatgag
    13441 attatagatg aagttatagt tagtttcatg aaaggtccta gaagctttac agctgaggat
    13501 acagtagaaa taaactgtca tggtggtatt gtagctacta ataaagttct acaaaatgta
    13561 ataaaagctg gtgcaagact tgctgagcca ggtgaattta caaaaagagc ttttttaaat
    13621 ggaagaatag atttaagcca agcagaagcg gttatggaca taataactgc taagactgaa
    13681 ctttcaatga aatcagctat gactcaaagt caaggaagac tttctactga gataaacaat
    13741 cttagaaaag aggctttaga tatattagct ctaattgaat atgctgttga ctttacagag
    13801 gatgacgaag aaccagatga aactatacaa gttaaggtaa aagagaatgt tataactctt
    13861 agaggtaaag taaataattt aattgatact gctgacgaag gtaaacttat tagagatggt
    13921 ttaagcatgg ttatagtagg aaaacctaac gtagggaaat catcactttt aaatgcttta
    13981 ttaaatgaaa agagagctat tgtaactgac atagcaggaa caacaaggga cgtaatagaa
    14041 gaatatataa atttagatgg aatacctgtt agactagtag atacagcggg aattagagaa
    14101 actgaagatg ttgtagagaa aataggtgta gaaaaatcta aagaaaaaat caatgaagct
    14161 gatttagtta tactaatgtt agatacaagt agagaactag atgaggagga caaggaaatc
    14221 attgattaca ttaaagatag aaaatacata gtacttttaa ataaggttga tttagataga
    14281 aagttatcct cagaaattgt ggataactta gaaaataaaa tagaactatc tgcaaaaact
    14341 ggttttggta tagatgattt aaaatcaaag ataaaagact tattctttaa tggatcaatt
    14401 gatgctgaaa gtgtaatggt aactaatact agacataaag aggctttata tagagcttct
    14461 gaaaacttag atggtgcatt aaatggactt aataataatg agtttttaga tttagtatca
    14521 atttatgtta catctgcttt aagagcctta ggagaaatta ctggagcaga gctagaggaa
    14581 gatttagtaa acaaaatatt tgcagaattt tgttgtggaa aataggagaa tgatgaaata
    14641 tgagatataa tgcaggaagt tatgatatag ttgttatagg tgctggtcat gcaggttgtg
    14701 aggcaggatt agcggcagct agaatgggat gtaaaacttt agtttgtaca ttaacattag
    14761 attcagttgc tatgatgcca tgtaatccta atataggtgg aactgctaag ggacatctag
    14821 ttagagaaat agatgcttta ggtggagaaa tgggagtaaa tatagatcat acatttatcc
    14881 aatctaaaat gttaaataca tcaaaaggac ctgctgtaca ctctctaaga gcacaagctg
    14941 ataaaaagaa ataccaagaa agaatgaaaa aggttttaga aacacaagaa aatcttcatt
    15001 taagacaatt agaagtagtt tctgtagaag ttgaagatgg aaaaattaaa ggtgttctaa
    15061 ctaaaaatgg tgcatttttt gagtgtaaag ctgttataat gacaagtgga acatacttac
    15121 aaagtagaat aataatcgga gatgttagtt atagccaagg tccaaatgga cttagcaatg
    15181 ctaatgaact ttcaaaatca ttaatagatt taggaattga ccttagaaga tttaaaactg
    15241 gtactccagc tagagtcaac aagagaagtg ttgatttctc aaaaatgata gaacaaccag
    15301 gagatgagga aataatacca ttctcattta taagtggaaa tatagataga gaccaagttt
    15361 catgttggtt aacatatact aacgaggaaa ctcataaggt aattcaagaa aatatacata
    15421 gatcaccaat gtataatgga agcataaaag gtgttgggcc aagatattgt ccatcaatag
    15481 aagacaaggt aatgagattc caagataaag atagacatca aatctttata gaaccagaag
    15541 gtgatgatac agaagaaatg tatgttggag gaatgtcatc atctcttcca gaggatgttc
    15601 aagttcaaat gataaaaaca gttccaggtc ttgaaaatgc tgagattatg agaactgcat
    15661 atgcaataga atatgattgt atagatccaa cacaattaaa ggcttcacta gaatttaaaa
    15721 atattgatgg attctttagt gctggacaaa ttaatggtag ctcaggatat gaagaagctg
    15781 gagctcaagg aatagtagct ggtataaatg cagccttaaa agttcaagga aaagatccta
    15841 tgatacttac aagatcagat ggatatgtag gagttttaat tgatgattta attactaaag
    15901 gtactaatga gccttataga atgatgacat caagagctga atatagatta ttattaagac
    15961 aagataatgc tgatttcaga ttaactgaaa ttggacacaa tgttggatta gttacagaag
    16021 aaagatggaa taagtttcag gaaagaaagc aaaacttaga acgtgagctt gagagactta
    16081 aggaacttca aataactaat aaaacagaaa ataatgagaa aatagttgaa ttaggttcaa
    16141 cagagcttaa aaagccaata agaatgtatg aacttataaa aagaccagag ttagactact
    16201 tctcattagc atgtttagac cctgaaagac ctgatttacc aaaggatatt ggggatcaaa
    16261 taaacataat tgctagatac gaaggataca ttcaaactca attagaacaa gttgctcaat
    16321 ttaaaaagtt tgagaaaaaa gttcttccag aggatttaga ttacaatgac gtaaatagtt
    16381 taagaataga agctatacaa aagctaaata aaattagacc tttaaatata ggtcaagctt
    16441 caagaatatc aggggtatcc ccagcagata tatcagttct gttaatattc ttagagcatt
    16501 atagaaaaac tggaaaaaat actgataact aaaagttatt cagaagtttt caacagtacc
    16561 tgtggataag tcttgtgtaa aacttaaaaa aggtactgtt ttaccccttt taaaaagtta
    16621 tccacatgtg gataacttta gttttttagt agaaaataaa attattaaaa taaattattt
    16681 atactcttaa agttttggag gattaagaat gcaatatttt gatttaatga aaaaagcttg
    16741 cgatagtgtg ggtatggaat ttaatgaaga caaatatcaa aaatttatgc tttacaaaga
    16801 tttattaaag gaatggaatg aaaaaataaa tttaacggca ataacagagg atgaagagat
    16861 agtaaaaaaa cattttatag attgtataaa agcttttaaa gcagatgagt ttaaaaaagc
    16921 taaaacagta atagatgttg gaacaggtgc agggttccct gggttaccta tagctattat
    16981 gagagaagat gtagaggtta ctctattaga ctcattaaat aagagaataa attttttaaa
    17041 tgaagttgta aataaactag cattaaaaaa tgttgagact atacattcta gagcagaaga
    17101 tggtgcaaga aaaaaagagt taagagaaaa ttttgatatt gctacatcaa gagctgttgc
    17161 taatatgtgt gttctaagtg aattttgtat tccatatgta aaggtaaatg ggaattttat
    17221 agctttaaaa ggcccaaata taactgaaga gttaaatgat tcaaagaatg ctattggtac
    17281 tttaggtggt aagcttaaag gtataactga ggttgaaatt gagggaacag acttaaatca
    17341 taacttagta atagtagata aaataaaaag tacgccaaag acttttccta gaaaagcagg
    17401 taatgtaact aagaaaccat taaaataaat ataattttta aaatactttt tagtttaaga
    17461 aaatattata attgaaagtt ataaaagctt ttaaaaaata ataaatatat tgaccataaa
    17521 aataggaatg tttcacgtga aacattccta ttttttcatt ttttaatcag aaataaccaa
    17581 tatcatcttt tattatgtaa tgtaaaaata tataataaaa tagatgtgtt agaagggagg
    17641 tggatgttca agaataagat attcttggat gagtttaatg aaaacaattt gtgtttttaa
    17701 tcaaaaaggt ggcgttggaa aaacaacaac aaatataaat atatgttctt acttagcaat
    17761 gcagggattt aaggttttag ctatagatat agatcctcaa gggaatacta caagtggatt
    17821 aggtatagat aaaagaaaat tagacaagtc tatatatgat ttattaactg ctggtgaaga
    17881 gatagaaaca ataataatga aaagtgaatt agttgaaaat ctttatgtag taccagcaac
    17941 aatggaatta gcaggtgctg aggtagaaat aatagataga aaagatagag aatatatact
    18001 taaaaatgaa ataaataaaa ttagagataa attcgattat atatttattg actgtcctcc
    18061 atctttagga gttctaacta ttaatgcttt agttgcatct catagtgttt taatacctat
    18121 acaagcagaa tattatgctt tagaaggggt aggacaatta ataaatacaa taaatttagt
    18181 taaaaagtct ttaaataagg atcttgatat tgaaggtgta gtaatgacta tgtatgattc
    18241 aagaactaat ttaagtacag aggtttataa ggaagtaaaa gagtacttta aggatagatt
    18301 atatgaaact actattccta gaaatgttag actagctgaa gctccgagtt ttggattgcc
    18361 aatatgttta tatgatgaaa gatgtagggg agctaaatct tatgaaaaac taactgagga
    18421 attcctaaaa agacagggag tgaatatata gatggctaaa aaatttggtt taggaaaagg
    18481 attaggagcc ttaataccag aagataatat taaggaagaa aaaaaagaaa aaggtggaat
    18541 aataaacatt gagcttaaag atataaaagc taataaaaaa caacctagga agttttttga
    18601 caataataaa ttaaatgaat tagctgagtc tgtaaaggaa catggtataa ttcaaccttt
    18661 aatattaaag aaagaagaaa agggatattc aataattgca ggggaaagaa gatatagagc
    18721 tgctaaaata gcagaattaa cagaggttcc tgctttaata atggatattt cagatgaaaa
    18781 acttctacaa gtatctttaa tagaaaatat tcaaagagaa gatttaaatc ctatagaaga
    18841 aggattagca tatgaaagat tattaaaaga atttgcttta actcaagaag agctttcaaa
    18901 aacaatggga aaatcaagaa ctacaataac aaatacaatg agattattaa agttagatga
    18961 tagagttaaa gagtatgtaa tggaaggagt tatttcagaa ggacatggaa gagctattct
    19021 acctttagaa aatgaggcac aatatgaaat agctcaaaaa ataattgata actctctttc
    19081 agttagagaa actgaaaaat tagttaaaaa gatattaaat gatgctgttg aagaaaaaac
    19141 atcagaaaga agaaaaccac ttaatccata ttacaaagat gttagagata gacttcagga
    19201 tcactttgga actaaagtta atttaaatgc taataagaat aaaggtaaga tagaaataga
    19261 atactattca gaagaagact tacagagaat actagacatt ataaatattt aaaatgtttc
    19321 acgtgaaaca ttttaaaaat acgaagggat gataaattac atggagaatg tactaaatat
    19381 acttacacaa tattcaactt atataacaat aggattaata gtattagttt taatacagtt
    19441 tatactatta atagtagctt taagaagttt aagcaaagta gaaaataaat ttagaaaaat
    19501 tatgagagga gttaataata aaaatttaga agaacttatt aactcttact tagataaagt
    19561 tgaagaggta aaaaaagatt ctgaagaaac attagaaact aataaaatgt taaaagctca
    19621 agttgaaaaa tgtactcaaa aagtatctgt tataagatat aaagctttcg aagacgttgg
    19681 aagtgattta agtttttcag ttgctttatt agacggagaa aataatggag ttattttaac
    19741 tggaatatat ggtagagatt atagtacaac atatgcaaag ccaatagata aaggaatatc
    19801 tagatatgat ttatcagaag aagaactaca tgttctaaat gctgcaatga ataaataaat
    19861 ttaagaagaa atataggtta tattaaaaca acgattttta gttgatttta atataaccta
    19921 tttttgtgtt aaacaaaatg tttcacgtga aacatttcaa caaatagggg agtatataaa
    19981 aaactatatg agtatttatg gcgaatttta taaaatattt tctaaagttg aatctaatat
    20041 attcttttta aatcctacgg attttaatat gcaatgatga attccattag atatagcatt
    20101 tgcaagtaac attacagtgt ataatctagt attttgtaaa accataaatt ctaaggaacc
    20161 agaaatattt actattccaa gtatgcttaa gtctcctact tcaggcaaat ccttgtttaa
    20221 tgctattcca ggtttaagag gtctcttttc aataagtacg tgtcctacat tatcaacagc
    20281 tcctaaacaa gcatcaatgg ctataataaa aggtttttta tgttttgatt taattatttt
    20341 tactgtctct tttaagtttc cagcatggac agtttcttct aatgttccat atatgaaaat
    20401 attattttta cttaaaaatt taatcttatc accaattaat gggccaagag catctcctgt
    20461 ggagcgatca gatccaatac atataaaaat aattggtcta ttttctttta taactggaga
    20521 aagttctctg caaagataat ctctaatttt aaaagaagct ttagaatcta aagaatttat
    20581 ataaaaactt gtacacatta tactaacctc cttacttgaa attattacca aaaacaaaaa
    20641 gtctatacta taaaacatta acttttttaa atatattgaa aaaaataatt agaaagatac
    20701 aaaatactat tcctaaaagt ccatttaatt ctaatcctat aaaaatacaa attaatatta
    20761 ataaagggtg tattcctaaa gtagatgcca ttaatttagg ttctaaaaat tggcgtaata
    20821 aaaaaataaa tatatacaat ataaataatg aaatagctga aaaaatattt cctgagttat
    20881 atgaaataaa aataagagga aaataaatta agggcatacc tagcacaggg agtagatcta
    20941 ataaagcagc aattacacta agcaataggg catatttaat atttaaaaaa gaaaatccta
    21001 aaaaggttaa tacaaaagaa ataaaaatta aaaataaata agagaaacag tatgtcaaaa
    21061 acattttttt tatttcatat aaaatagtta aaattctatt gctgtaatta gagttaagat
    21121 aattagacaa agttttacta aagttaatag tgtttaattc ttttgataaa aaataagttg
    21181 atattacagt aaaaataaga agcatgaaag tataaggtaa ataacttatt aataaaaaaa
    21241 ctttttgaat tagttcagtt ccaaaagaaa gaataagtac agttatttct ttaaatgaag
    21301 atattaaagt attttttatt aagtttaaaa ctgcattatc aataaagttt acttcaatcc
    21361 aattttgtag tgatataaaa taattttgaa attccttaga gttagtattt attatttctt
    21421 gaatatagtt tgttaaggat gaagattcat aagctaaaga tgtaataaac aatgtaaata
    21481 aaattattat tattaagaaa aaaataaatg tacttataaa ggaagcaagc ccaacattta
    21541 atttaaattt ttttattaaa aattgagtgg gtgtctttag tagcaatgaa aatatgaatg
    21601 ctagtagaaa gggcaaagta tattttaatg tattaaaaaa tgtaaaaaat ataagtgtgt
    21661 ataaaataaa aaacacactt attttttcac ttttgattat taaatttttc ataggtcaga
    21721 agtctacctc cttgaaatta attcttttat aacctcaaca aatctataga cttcttctat
    21781 tgtattaaag taccctaaac ttattcttat gcttccagct ggatatgaac caactgtctt
    21841 atgagcaagc ggagtacaat gaattccagt tctacatgtt atgttaaatt cactatctaa
    21901 caagaatcct gtaaattcag gatccatacc ttctatattg aaagatattg ttgaagtttt
    21961 ctttttggca tccataggac cataaatctt aacttctgga atttcacata ataaatccat
    22021 agctttttgg cataatactt cttcttttgc ttttatattt tcaatgcctt ctttttctat
    22081 gaattttatt ccctctagaa gccctattat tcctggagtg tttaaagttc catattcaaa
    22141 tttatcaggc aattcttgag gatgttctat tagtgatgaa ttacttcctg ttccgccaga
    22201 aaagatattt ttacatatag aatttaattt atcatctata ataaaaccac ctattccttg
    22261 aggtcctaaa agagacttat gacctgtaaa ggctaatgca tttaaattaa gttcagtcat
    22321 atcaattgga ataacccctg ctgtttgagc agaatctaaa ataaaaaaga tatcattttc
    22381 tttacaaagc ttccctattt ctttaattgg ttgaattgtt ccaactaggt ttgatgcatg
    22441 agataaaatt ataagctttg tgttattttt tattttttct tttatctttt caactgacac
    22501 taaaccttca ttattacatt gaactatatc tagttctaca ttaggaagct cctcgctaat
    22561 ttttactaaa ggtcttaaga cagaattatg ttccatagat gtagtaatta tatgccaatc
    22621 agatttaatt attcctaaaa gtaacatatt taatgatgtt gtaatattat ttgtgaaaat
    22681 aacattttca cttttaggaa aattaaagaa tttagctatt tcatatctac attcatagac
    22741 agctctacta ctttgtagag ctgtagatga ggatcctcta ttagcacttc cgccttcaaa
    22801 actcatataa tcaaacatag cttttattac agagtcaggt ttagggaaag tagttgctgc
    22861 attatcaaaa tatattttat tcataagcaa aaacctcaca tgaaaatttt aaatattccg
    22921 tatatagtaa atcctaatcc aagtaaaatc ataaaatatt ttacactttt atcagcttta
    22981 ccagtaagtt ctttttttac tagacttcca cctttggcag ccataaaatt taataatata
    23041 aaccaagcta aagttgttat aaacatagca cttagtgcta ctaaaaagaa atgtctacct
    23101 gaaagtttcc atatactcat tattccagca cttacagcca tccatactga taaagtcata
    23161 gggtttacaa aggttattaa aaaaccattt ataaaaccat taccagtttt atgactatct
    23221 atagtttcaa tacatttttc atctaaactt ttttgagaag atttaaatat taaattaact
    23281 attataagaa ctaatcctga aataatccaa aaggcacatt ctacatacct atttaaattt
    23341 aataattggg ctactccgaa gtttattaat aatatgtaca aatagtcagc agctattgcg
    23401 cctaatgaaa cttcaaagcc ttttctaaat cctttatcta gagattgtgt tgcagcttct
    23461 attgcagctg gacctaatgg tatagaaact aataggcctg ttacaattcc agataaaata
    23521 gcctttaaat agaaaaaaga catactcaaa ctccttttac agttgttttt ataaatttac
    23581 ataattatat tatagttcaa aatagaaaag ctttcaccct tattttgaaa gtgttaactt
    23641 ttatgatata tactaataat atataataat aaaattatgt gattttagga ggaaaatagt
    23701 atgaaagact ttattatggt ttttaataat acacacgagg ctatggaggg agaaagaata
    23761 cttaaagaaa atggaatagc gtttataata atgccaactc caacttacat aacaaaaagt
    23821 tgcggtataa gttctgcatt taataaagat caattagaaa agataaaaac tctagaaatt
    23881 aaatttaaaa atatatattc aataactaat aaaggatttg aaatatataa ataattattt
    23941 atttcaaaat cataaggagt ggtttaatgt tattaagtag tttgcttgga attaattttg
    24001 aatttgacca cattaatctt ggaaaaataa gtatttcttt atctactatt gattttctat
    24061 taaaaaaatt tataacaata tgtataatag ttatgttcat gacactagta attaatatag
    24121 gaaatagatt aatagataaa gctgttcaaa aacaattaga tacaaatttt aagttttctt
    24181 tagataaagg aaaagcttat actttaggaa ctttattaaa aagtatttta atgtattctg
    24241 tatattttat aggtataaca actatactat ctattatatt tggaaatata tcttgggctt
    24301 ttgcctctgt aggaggagta gccgtaggtt taggagctca aagttttgtc aaagatgtaa
    24361 taaatggaat atttatactt tttgataacc aatacaatgt tggggattat gtaaccatag
    24421 aaggagtaag tggaattgta gaggtaatag gtcttaggac aactgagctt agagatcaag
    24481 atggttctct tcatattatt ccaaatggaa tgataagaat agtaacaaat aattgtagag
    24541 gtgatatgag agttcagatt gatattggaa tttcatatag tgatgatata aataaagtaa
    24601 taaacactat aaactcagtt tgtgatatat ataataaaga aaataatgat ataacagaac
    24661 cattaaaggt ttggggagtt acagacctaa caagttctag tgttaatctc agaatttgtg
    24721 gaaaagtaaa atcaatgaaa ctttgggaaa cagaagtaga gttaagaaag agaataaaaa
    24781 tgactcttga taaaaagaaa tatatgaaag aaataattac aggttaattt atataaattt
    24841 tattttagag gaggttattt aatttgataa acaaatttta tgttggtgat atagttaaaa
    24901 tgaggaaagt gcatccttgt ggaagtgatg agtgggaagt tataagagtt ggagctgata
    24961 ttaaaataaa atgcttaggt tgctcaagaa ttgtaatgat tcctagatct aaattccaaa
    25021 agggagtaaa aaagattata aaatcaaatg ctccagagga agaaattaat gaataaaatt
    25081 tttaaaaaat aaatttataa aagttatatt tacattaata aagagggttg caataaccct
    25141 cttttaatgt taaaatattt aattgtaatc cctgctatgc tagagcatag ccgtagtcca
    25201 gagggaggag gtgtaaatga tgagaaaata tgaaactata ttcgtagctc acccatcatt
    25261 agacgaggaa gctgttaaag ctttaatcga aaaatttaag ggtgttatag aaaacggaaa
    25321 tggtacagtt gacaacgttg atttctgggg taagagaaaa ttagcttacg aaatcgctaa
    25381 agttaacgaa ggttactata ctttaatcaa cttcactgct aacccagaat taccaaaaga
    25441 gttagacaga gtattcggaa taactgacgg aataatcaga cacatcatcg ttaaagaaga
    25501 gcaataagag gtggtcgtaa atgaataaag tagttttaat tggtagactt actaaagatc
    25561 cagagcttag atttgcagca ggaagcggta cagcagttgc aagatttaca ctagctgtaa
    25621 atagacaatt caaaaaagat gaagctgatt ttatcagttg catagctttt ggaaaaactg
    25681 gtgagaccat agctcaatat attactaaag gcagacaatt ggctgtttca ggaaatataa
    25741 gaactggaag ttatgaagct caagatggaa ctagaagata tactactgac gtagttgttg
    25801 aaagttttga ctttatcgat tcaggaaatg gcggagcaag aggaaactca ggctttggtt
    25861 caggaaatga tttcggtggt tctttcggta tgccagacaa ttcatttagt gattcatcat
    25921 tcaatagtaa tgatgatatg acaccaattg atgatggaga cattccattc taattcagat
    25981 tttaggtaag gagggaaata gtgatgagta acgtaaaaag aggcggaaaa ttcagaagag
    26041 caagaaaaaa agtttgcata ttctgcgttg ataaagctga atcaatagat tacaaagatg
    26101 tagctaaatt aaagaaatac atcacagaaa gaggtaaaat attaccaaga agaatttctg
    26161 gaacttgtgc taagcatcaa agacaattaa cagatgctat caagagatca agaaatatcg
    26221 cattattacc attcacaact gagtaatggt tgcaaacccc tgatattttc aggggttttt
    26281 ttattatata ttaaattatt ataataatct aagtttttaa gtagtcttat taaatggttt
    26341 aagaagattt tatttgtgtg gtagttatat taaagtgtat acttattaaa acatgtacac
    26401 tttaatataa atgctactat aatttagagt ttttaagttt tctctgaatt aagtctcatt
    26461 tattgcataa gactaaaaaa ataaataaaa taaaggtata taataaaggg tataattggg
    26521 agggaaatat gaaaaaagat aactttaata tcatgggaga tataaagatt attgaagaaa
    26581 taaaggctca aattatctgc atattaggag aactatttac acttttaaca aaaggaagta
    26641 atgtagcaaa ggatgccata gtaaactgca tagcatcttt aattatttta ttatacattt
    26701 tagcagataa gttaggtcat tctgcaatag aagtagatga gacaattaaa aaaagtttaa
    26761 agattggtat agttgaagag gataatttag aaaagcaagg aggtaactta actaaattat
    26821 ttaatcattt aaaagaaaga agataattta tttggaggaa catatggaag aaaagaataa
    26881 gagtaaatct atagttgaag ctggaataat gtcagcaata acatttgtac ttatgatgct
    26941 tactagctta ccaggaatag gtacaataat aagctttgct attccaattc ctatagcttt
    27001 attgtattta aagtataatc ttaaaacctc tatttgttct attgttatta gtggaatttt
    27061 gattggattt ttcatggggt ctatatatgg aattcaatta gctataacaa atggaattat
    27121 aggtttggtt ttaggaacct gtgttagaaa gaagttttca ggaataaaat catttatata
    27181 tttaattgta gcaaatacaa ttggtgtatt aatagaaata gcattattat tttcagtatt
    27241 tacaggaatt acatttaatc aatatataaa tgacttagta aatgttttta atgaagctgc
    27301 taaaataagt gaacaaatga ttggaagttc acaagccaac actcaaatga tagaacttat
    27361 gaagtctatg gatgcaagtt ttataattaa tatgatacct atggtattag ttgtggttat
    27421 aataatgaga gcatttttaa gttataccgt tggaaagtat attattaata aattaggatt
    27481 taaattaaat agcttaccac ctttttcaaa gtggtttttt aacccaatat taattgcggc
    27541 agttgtatta ataaatcttt ttgttatgct tttagtaagc aagggagtaa taaataataa
    27601 gggtatgtac atgcttacac taaacttatt atttgctatg cttataattc aaggattggc
    27661 tgtagtctca aatttattaa agtatagata tagatttagc aattttttaa tagtttttat
    27721 tagtatatta atggttactt caataccaca attatttgga atattgggat tggttgatgt
    27781 attaattgat ataagaggtg tagatccaaa tagtttagga agttacatta aggagaaact
    27841 aaaaaggaaa ttgcaataac taatatttat atttagtaag gggctgttaa aatgaaatca
    27901 atattggaag attataataa atttttagct tactttgcat ttgtagtttt agcatgctta
    27961 gtattttgga aattaaactt taaaatagta tctttagtaa tagcttttgt atatataata
    28021 tatttaattt ataaaatatc ttacttttta agagaagaaa aggatgagga agaaaagata
    28081 gataaattat ataaaaatgt agatgagaaa gttaaagaca atatttttaa tttgattttc
    28141 ccattaacta tattaaattc tactggtgaa ataatatggt ataaccataa gtttagtaaa
    28201 cttataaata gtgaagattt gctagaaacg aatttagtat ctgttataaa aggtgttgct
    28261 ttagataaga taattagaac aggaaaagaa aattctcaaa atataaaaat aaaaagtaat
    28321 atctatgaag tttatagtga gggtttaaaa ttagatgaat cagataaaaa atatttctta
    28381 gtatttttca atgatgttac ttatttaaag aaggccacaa aagaaagtat aatgctttta
    28441 gaagttgata attttagtga agttttaaaa acaatagatg atgacaaaaa accattactt
    28501 atagcagaaa tagaaagaac aataaataac tatggaaatt cactaaatgc catgataaaa
    28561 aagtatgata atagtaaata tattttatca gtagatgatg ctattattgg caaggaaatt
    28621 tctaaaaagt ttgatatact agatactata agagaaatta acttagggaa tgaaatagaa
    28681 gtaaccttaa gcataggtgt tggaagaggt ggagaaactc ctgcacaaaa tcaagatgat
    28741 gccacaaagg ctaaggaatt agctttaggt agaggtggag atcaagcagt tattaaatca
    28801 ggtaaaaata ttgagttctt tggaggaaat gctaaggaac ttgaaaaaag aacaagagtt
    28861 agagctaggg ttgtagcaca tgtattaaaa gaacttgtct atgaaagtag tactgtttat
    28921 ataatgggac ataaaaatcc tgatatggat tgttttggtg cttcatttgg tttagcttca
    28981 gtagtgaaga agttaggaaa aaatgttaat atagtattag aaaaagatac taatgctata
    29041 gaatttttct tagatagagt taaagaaaaa gaagaatata gtaaactatt tatatctact
    29101 gaagaagcta aaaagaatat agatgacaat actttattaa taatagtaga tgttaatagt
    29161 gcaaactatg tattagatag agagttaaca gaacttattg aaagggtggt tgtaatagac
    29221 catcacagaa gaagtcctaa ggatataaag ggagcactat taaattatat tgaggtatat
    29281 gcttcatcta catcagaact tgttactgaa atggttcagt atatgttaga taaacctaaa
    29341 ttaaatagaa ttgaagcaga aggtctttta gctggaattt atatagacac taagaatttc
    29401 tcttttaaaa ccggtgtgag aacctttgaa gcggcttcat ttttaagaag attaggtgca
    29461 gacacagtag agattaaaaa aatatttagt aataacttag aaaattatat agcaaaagca
    29521 gaaataataa aatctgcaaa ggtagaaaat aaaatagcaa tagcagtttg cccagaaaat
    29581 attactgata ctgttgtggc agctcaggct gctgatgaac ttttaaatat tactggaata
    29641 caagcttcat ttgtattaat aaaattaggt gaagatgttg ctataagtgc aagatcttta
    29701 ggggatatta atgttcaggt gattcttgaa gaacttggtg gcggaggtca tatgactatg
    29761 gctggcgcta agattaagga taccactatt gacgaggcta tagaaaaaat aaaagaagct
    29821 attgcaaaaa actcaaggga aggtgaataa aatgaaagta atattacttc aagacgttaa
    29881 aaaattaggt aaaaaaggcg atgttataaa tgcatcagat ggatatgcta gaaacttttt
    29941 atttccaaag aaattagctc aagaagctac tgataataat cttcatatat taaacaataa
    30001 aaaagagaac gaaagaagac aaaagttagc tgaattagaa gctgctcaag ctttagctgc
    30061 tgatttaaaa gataaagtaa taaaaataga tggtaaggct ggagataatg gaaaattatt
    30121 cggagctata actagcaaag atgtagcagg acttatcaaa aaacaattta atgtagaagt
    30181 tgataaaaag aaaattgtta tggatacaat aaaaattgca ggaacttata acatagaagt
    30241 taaactatat ccagaagttt caacaaaaat gaaggttatg gttgtccctg ttcaataaga
    30301 ggaggattct attttgaaat caattaaaga attagaggtt ctacaagatg ttatgaatag
    30361 tgaacttgga gtagaatctc aagttgaagc actaaaagat ataataaata atatattaga
    30421 cgaaggtgca tttagagcga gagttataag atttaaagta caaaattata taaattcaac
    30481 agatccttat gaaagacttt atggattaag taaaattgtt tctgaaggaa agggtttaag
    30541 tgaagttcca actgaagaaa ctataaatga agctttagaa gatgtttgtg ctatgatatc
    30601 agatgctatt gctagaagat atgttcaaaa taaaatagaa aaagaagttg aacaattctt
    30661 aatggaaaag caagaaaagt atgttgatga acttagagta aacataatga aaaagaaaaa
    30721 aggtccagaa aatactaaga cagagaaaaa gcttgaggaa cttgaagaac tagatgagag
    30781 agttccaaat aagaatataa tgtctttatt aagacctgat tcatttgatg aggtagttgg
    30841 tcaagagaga gctgttaagt cacttctttc aaaactagct tcaccatatc ctcaacatat
    30901 aatactttat ggacctccag gggttggtaa aacaacagct gctagaattg ctctagaaac
    30961 agctaagaaa ctaaaatcaa ctccatttga tgatagatca aaattcatag aggttaatgg
    31021 tacaacttta agatgggatc caagagaaat cacaaaccca cttttaggtt cagtacatga
    31081 tccaatatat caaggtagca aaagagactt agctgaaata ggagttccag aaccaaaacc
    31141 aggtttagtt actgaagctc acggtggtat attatttata gatgaaattg gagaattaga
    31201 tgaaatactt caaaataaac ttttaaaagt tttagaagat aagagagttg aattctcatc
    31261 atcttactat gatccagatg atgaaaatac acctaaatat ataaaatatc tttttgataa
    31321 gggagctcca gcagattttg ttctaatagg agcaactact agagagccgg gagaaataaa
    31381 tcctgcttta cgttcaagat gtacagaggt ttattttgaa ccactatcat caagagatat
    31441 tgaaaagata gtattaaatg cagctaagaa gcttaatgtt aagcttgaag aaggtttaga
    31501 aaagaaaata gcttcttaca ctatagaagg tagaagagct gtaaatatat tagcagatgc
    31561 ttatggtcat gctatttatg gcttagaggg agaagttcca gaagacttag aaataacttc
    31621 aaaggattta aatgaagttg taagcatagg aagatttact ccttatgaaa tactagaaaa
    31681 cttagaggaa aaagaagtag gtcatgttta tggacttgga gtttcaggat tcttaggctc
    31741 aacaatagag attgaagcca ctgcttttaa agctaagaaa aagggtgctg gaaaaataag
    31801 attcaatgat actgctggtt caatggctaa agattctgta tttaatgctg catctgtaat
    31861 aaaaaggcta actgataagg atataaatga ctatgatata catgttaacg taattggcgg
    31921 aggaaagata gatggaccat ctgctggggc tgccattacc atatgtataa tgagtgcttt
    31981 attagaaaag ccaataagac aagacttagc tataactgga gagatttctt taagaggaaa
    32041 gattaaacca gttggaggta tatttgaaaa aatatacggg gctagaagaa agggaattaa
    32101 gttagtaact gttcctaaag ataatgaaaa tgaaatccct aaaggattag aagatataga
    32161 agttaaagct ataagttcta tagaagagct tatggaaata gcttttaatt aaaaataaag
    32221 aacctagatt tatttctagg ttttttattt tgtcttttaa atttacatag tataaataaa
    32281 actataggtt ttttacaaga tttcatgtat tatatatatt gtgataaagc atttttctag
    32341 ttatttttct atatatatat attgataaat tttaatgtat tatatatagt ggagtagtat
    32401 tttattaatt agtttttata attaaaaaat taattatgta ttatgtcact aaattatatt
    32461 tttttgtata aagtaattta tttgggatat attggataat agttgatgaa attataatag
    32521 aatatttaat tataaaaagt ttaagaaaag tttataaggt ttagtttatc ttataaactt
    32581 ttaatgatat tagaaattta ttttaaggga aaggaggaaa attaggaata atggaagcac
    32641 cagtaatgaa gagtttgcca caaagtatag aagcggaaca atctgtttta ggggcaatga
    32701 taatagatag aacaaccata gctcaagccg ctgaggtttt aaataaggat tcattttata
    32761 gagatgctca taaggtaata tttcaaagta taatagaaat gtaccaaaga gatcaagctg
    32821 tagaccaatt aactctttta gagtatttaa aatctactga tagacttgaa aaagccggtg
    32881 gtataagtta tgtaacagag ataagtgcat ctgtacttag tacagttaac ataaaatcat
    32941 acataaaaat agttgatgaa aaagctatgc ttagaagact tataaaatcc tctacagaaa
    33001 ttatagagga ttgttataac aatcaagaag atgttgaaaa agttatagat tcagcagaga
    33061 agaaaatatt tgatttatct gaaaagagat catcaaatga ttttgagcct ttaagcgttg
    33121 ttcttgaaag aggttttgag caaatagagg ctatatttaa taatgaaggg gaacttacag
    33181 gtgttggttc tggatttaaa gatttggatg agatgacatc tggattccaa aaaggggata
    33241 tggtgcttat agcagcaaga ccatctatgg gtaaaacaac ctttgctctt aacatagcag
    33301 aacatgctgc tttaagagag ggaaagagtg ttgttatatt ctctttggaa atgtcaaagg
    33361 aacagcttgc atataagtta ttatgttcag aggctaatgt ggatatgtta agtcttagaa
    33421 caggtaaatt aactcctgag gattgggaca atatagctag agcatcaggc ccactagcat
    33481 cagctaagat atacatagat gatactgctg gggtttctgt aatggaaatg agatctaagt
    33541 gtagaagatt aaaaatggaa tatggaatag atcttatact tatagactac ttacaactta
    33601 tgagtggaag tggtgaaagt agacaacaag aggttgcgga gatttcaaga aatataaaag
    33661 ctatagctaa ggaaatggaa tgtccagtta tagccttatc tcagctttct cgtgctccag
    33721 agcaaagagc agaccataga cctatgttat ctgaccttag agaatctgga tcaatagagc
    33781 aggatgctga ccttgttatg ttcttatata gagatgagta ctataacaag gaaacagaag
    33841 ataaaaatgt tggagaatgt ataatagcta agcagagaaa cggtccagtt ggaacagtta
    33901 agcttgcatg gctaggtcaa tacagtaaat ttgcagatta tgaagctatt cataaggatg
    33961 tttaataaat ttagggtgta tcagaatgct gatatgccct ttaattttat tattggtagc
    34021 tacatattaa aaggtatata ataattaaag tatttataat tagatactaa atagttaaaa
    34081 tgactattaa gaaattaagt taataaaata attacaatct ttattaaaaa aggagaggat
    34141 aatatgaata aaaaaattgg attcataggt tgcggtaaca tgggaagtgc tatggtttta
    34201 ggaatattaa agtcaggtat tgtgtgcaaa gaaaatatta ttgcatcatg ttcaagagaa
    34261 tcaagtgcag aaagaataaa aaatgaatta ggaataaata caactcttga taacaaagaa
    34321 gtttgtaata acagtgacat aatatttctt gcggtaaagc catatatgta tgaaaaagtt
    34381 ataaaagaaa taagagattg tgtagactta aataagacta ttgttactat agcagcaggc
    34441 attaatttaa gtgatgttga aaaatggttt aagaaagaaa caaaaatagt taagactatg
    34501 ccaaatactc cagctttagt aggagaagca atgagcgcta tttgtcctaa tgaattagta
    34561 aatgaagaag aattaaaaga agttatggat atatttaatg cttttggaaa atcagaggtg
    34621 ttaagtgaga aagatttcca tggatttata gcactttgtg ggtcatcacc agcttatgtt
    34681 tatatgttta tagaagctat ggcagatgct gctgttagag agggaataag tagagataaa
    34741 gcctataaat tagctgctca atcagtatta ggatcagcta agatggtttt agaaactgga
    34801 gagcatccag ctaagttaaa agacaatgtt tgctcaccgg gaggaacaac tattgaagct
    34861 gttttagagt tagaagagac tggatttaga agctctgtaa tgagtgctat gaaaaaatgt
    34921 tcaaaaaaat ctaaaaaaat gtaatgtttt ttattgacaa aaaataaaaa aacatatata
    34981 ctttaatcat gataatcatt atcaattaga gattgattat tattcatgaa ctggataaag
    35041 acaaacataa caaacacaaa caaacataat aaaactccta caagaactcc tagattttct
    35101 aggagttttt ttcatttaaa ataataattt aattataaat ttattataaa gatgtattat
    35161 ttctaaaact cacagttttt atatactgta agtttatgct ttatagagaa aaacattgaa
    35221 aaaaaatagt aaatatgata acactataaa tagtattatt agaatttagg ggtggaagaa
    35281 tgtttaagag aagcaggggc atagccctgt atcatcaatt agaaacagag ctaattgatc
    35341 ttataaatag cggagaactt aaggaaaatg ataagttacc atctgaaaga gaattatgtg
    35401 agcaatataa tgttagtaga acaacagcta ggcaagccat tggagaatta gaaagaaaag
    35461 agtatgtata taaggtccat ggaaagggaa cttttatatc tcctaaagtg tataaacagc
    35521 aattattaaa attttatagt tttactgaag aaatgaaaaa gcttgggaaa aatccatctt
    35581 caaaaatatt atcctttgat ttaataagag ctgacaataa gatttctgaa aaattaaaag
    35641 tggaagaaaa ctcactggta tataaaatag taagaataag aatagcagat gaagttccaa
    35701 tgatggtaga gtgtacttat ttacctgaat atagatttat tgatttaaaa gagaagatgc
    35761 ttaaagaaaa gccgatgtat gatatattta gagaagtcta taatgttagt ctaactaagg
    35821 ctaaggaaag ttttaaacca atattaattt caaagagtga ttcaaaatta ttaaatgtgg
    35881 aagatggtac agctgcaatg agaattgaaa gagttacctt tgaaaatgaa agagttatag
    35941 agtatacagt aagtgtttca agaggagata aatttgaata tactgtagtt ttagaagaag
    36001 attaataaag taaaggggat gtatcaaaat ataataaaac ttacaaacag tatgaagaga
    36061 atatctagat gagctttaga atttaaatt
//
LOCUS       DAEVGI010000010        35534 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEVGI010000010 DAEVGI010000000
VERSION     DAEVGI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 35534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35534
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.010"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(4..402)
                     /locus_tag="K8M77_000497"
     CDS             complement(4..402)
                     /locus_tag="K8M77_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI7085025.1"
                     /translation="MNLKKIHHVAIIASDYKKSKDFYVNLLGLKIIREVYREERDSYK
                     LDLEIGDSQIELFSFKNSPKRPSYPEACGLRHLAFEVENIEKQVRELKEKGIKVEEIR
                     IDEFTGRKFTFFSDPDDLPIELYERGCIKI"
     gene            complement(514..837)
                     /locus_tag="K8M77_000498"
     CDS             complement(514..837)
                     /locus_tag="K8M77_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HBI7085026.1"
                     /translation="MSIAIMACRKVIGKCAASGCFKAYNNSTAAFSIYGENKEDLASF
                     FYCAGCKDTLVEGENWTHKVKQLKKNGVETIHISHCIESKCDDLKKYEKILEKEGFKI
                     VHGTH"
     gene            complement(1151..2299)
                     /gene="yiaY"
                     /locus_tag="K8M77_000499"
     CDS             complement(1151..2299)
                     /gene="yiaY"
                     /locus_tag="K8M77_000499"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBI7085027.1"
                     /translation="MSYKFFMPAISLMGADCLKDAGDQLGELGFKKALIVTDKVLGQI
                     GIVKKVTDVLDNKNIEYAIYDETKPNPTVKNVNDGLALLKEKECDFVISLGGGSAHDC
                     AKGIALLATNGGEIKDYEGVDKSKKPQLPMVGINTTAGTGSEMTLFAIITDEERHIKM
                     ALVDKHLTPIIAVNDPMLMLAMPKSLTAATGMDALTHAIEAYVSTAATPITDACAEKA
                     IELISNYLVNAVENGQDVEARDMMAYAEYLAGMAFNNASLGYVHAMAHQLGGFYNLPH
                     GVCNAILLPHVQEYNKSTSASRLAKIAKIMGGNIEGLTDEQGADLCIDMIKSLSQTIG
                     IPEGLGVLGVKESDFETLATNALNDACSLTNPRKGNLEEVIAIFKKAM"
     gene            2488..3345
                     /locus_tag="K8M77_000500"
     CDS             2488..3345
                     /locus_tag="K8M77_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7085028.1"
                     /translation="MDRFDRQLELADGIETEYLRILYYTFEKNYKDTYKSYEYKRLCT
                     IVDGEKNVRINNGKNIIYDSSKYMLLPPNSSVEMKIEKPTKALVLELNEDLINTVSEK
                     LSYELDVDLNKSILEKPFFNENNELIELTTKKIVETATKDTNNKEFLLDLYAQEITYS
                     LLNTKGAKDILSSDFNNPINRSIHIMKENLKNNITVSEIAYDLNMSLPSFSSKFKKIM
                     GMTPNDYLTNLKLNEAARLLKFSSVTEVAYDLGYENISHFIKLFKKKFGLTPKQYSLK
                     YSGEVIKIQ"
     gene            complement(3480..3860)
                     /gene="gloA"
                     /locus_tag="K8M77_000501"
     CDS             complement(3480..3860)
                     /gene="gloA"
                     /locus_tag="K8M77_000501"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBI7085029.1"
                     /translation="MASKMLHTCIRVKDLDKSLKFYEEALGLIETRRRDYPEYKFSLV
                     YLSDKEDGYEVELTYNYDQEKPYDLGNGFSHIAVGVEDIEAERERHISLGYEVTPMKG
                     LSDGAATYYFVTDPDGYKVEVIKL"
     gene            complement(3977..4645)
                     /locus_tag="K8M77_000502"
     CDS             complement(3977..4645)
                     /locus_tag="K8M77_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBI7085030.1"
                     /translation="MKNLIIKDLKNIRVIFIFYILTMILGYSPFFIDLPKDRYDFLIN
                     SVFIYFTLLASMISVNFIIAKNTNKGAMTNKLLRSLPIDARSVVGTSFILPILIFLIF
                     SLPNVLAGIGISFMLGEKIIVNTFNLLLTFIIFYIYASITFSMAIIYPESSVVSYLRS
                     VPLFILIIGLALVEKLLKNINYDVSNILNSLPLVVLILAVISIFILIVFYKFSLNKFI
                     RKEL"
     gene            complement(4647..5273)
                     /locus_tag="K8M77_000503"
     CDS             complement(4647..5273)
                     /locus_tag="K8M77_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBI7085031.1"
                     /translation="MKELLKLDFFIQKKYIKSSIIVFLFLGITFALTQGMNYLYTMGT
                     FIVFYLTIIPIEVERKSKFNIYLNSLPTKKSNYIYSKYLSTLIYIIVVNLFLYLFCTI
                     LGVISGSDFTLTLRAILPNIIVQLFYVSLTLPVYMIISHKFYRVFNTIFIILIMMISS
                     ILFNSLENENLQVLFNITNGVYGLAFGILALIISLIVSLNIYKKSEVS"
     gene            complement(5275..6138)
                     /locus_tag="K8M77_000504"
     CDS             complement(5275..6138)
                     /locus_tag="K8M77_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085032.1"
                     /translation="MENVLEIKNISKNYKGFSLKDVSFNIPKGFVMGLIGPNGAGKTT
                     TIKAIMNLINLDSGEIYIFGEDIKKNEISIKDRIGFVYDDCCAFEDFTIKENKKIISK
                     FYSKWDEEKFKYYVEKFELNENKKLKQLSKGQKMRFSLALALSHKAELLILDEPTSGL
                     DPVFRSELLDILFEIIGEEEVSILYSTHITTDLEKLADYITFLNKGKVEFSLEKDLLL
                     EEYFLVKGPLDILNKDLEKELIGLRKTRYNFEGLTNNMRNLKELFGEKIIFEKATLDD
                     IVVYYSKKDVK"
     gene            complement(6172..6543)
                     /locus_tag="K8M77_000505"
     CDS             complement(6172..6543)
                     /locus_tag="K8M77_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7085033.1"
                     /translation="MNIVVSNTSGVPIYEQIAKAIKNEILSGDLKENSALPSIRSLAS
                     ELRVSVITTKRAYEELERDGFIYTLPGKGSYVAEQNKELLMEEKLREIEEKLGEAIDI
                     ANSIGLNFNDLVGMLKTLKEL"
     gene            complement(6810..6983)
                     /locus_tag="K8M77_000506"
     CDS             complement(6810..6983)
                     /locus_tag="K8M77_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBI7085034.1"
                     /translation="MSNVCLCKGVSEEKIVEAVKNGALSFEEVGEKTGAGTGACCGAR
                     CKCKIEELIEENK"
     gene            complement(7173..7862)
                     /locus_tag="K8M77_000507"
     CDS             complement(7173..7862)
                     /locus_tag="K8M77_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI7085035.1"
                     /translation="MDSIGIGIPKMVSGTHYGNLQKVRGGKRTFGITPHIPGGFITPE
                     QLEKIAKVTKKYNGVIKITSGQRLLITNLKGEDLPKIWDELGMEPAVKKQNSVKNVEM
                     CPAGFCKRSKFNTIGTGMKLLNKYKHMDMPCRTKIGVAGCRNACGSVYSKDVGVIADK
                     TGFLVTAGGSAGHNPRMADILIKDVDEKEALRIVDKIFEVYKGEAEAGEKLGDFIDRI
                     GIEVFKEKTLA"
     gene            complement(8080..9417)
                     /locus_tag="K8M77_000508"
     CDS             complement(8080..9417)
                     /locus_tag="K8M77_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI7085036.1"
                     /translation="MDPSPSILPKIILILVLILINAFFAAAEMAMVSVNKSKIKMLAE
                     KGNKKALLLKKVLKSPGNFLSTIQIGITFAGFFASASAATSISETLAQFMYKLNIPYG
                     NEISVILITVLLSYITLVFGELLPKRIALQKPEEIALMAIRPINVISKISTPFVKILS
                     ASTNLFIKILGLNKSEDKETVSKDEIKSMISIGQESGVIDKAEKDMLDNIFEFDHKVV
                     KEVMTPRGEVFAIKSTTPNETIAKKLISEQFSRVPVYSETRDNIVGILYLKDFFEAVV
                     KVGVDNIKLDQLIRPAYFVIENKAIDDLFKELQDSKQHMAVIIDEYGGFSGIVTIEDL
                     IEEVMGDILDEYDDSENYIDKIDNNTYVVDGLLTLDKLNDYLNLNLESQNIETIGGFV
                     VNLIGNIPQSENQMVEYDNLSFQVCKTNKKRIEKLKIYLNNSTSFNSDVILNN"
     gene            9958..10374
                     /locus_tag="K8M77_000509"
     CDS             9958..10374
                     /locus_tag="K8M77_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBI7085037.1"
                     /translation="MSLAKLIDEKRKAKARAERNKKAKVAVTGAAVGTLAGVLGGLLL
                     APKSGKETRNDIKEGSKKAAQKLNDKSVELKENLNTQVAKGKENISDAKSKIKEYLNS
                     KKCKNIEEAVEENLNVPVEVADEATSLETEEVKESN"
     gene            10380..10715
                     /locus_tag="K8M77_000510"
     CDS             10380..10715
                     /locus_tag="K8M77_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085038.1"
                     /translation="MYIDLNFIYLLLALLGCIAIVYLILILNKVLKLVSNINSLLDQN
                     KDNLTKTIKDLPKIADNVNDACQNIKDISDVATETTADLIVAKENIESNVETVKEIAK
                     IINQVFFKK"
     gene            complement(10784..11263)
                     /locus_tag="K8M77_000511"
     CDS             complement(10784..11263)
                     /locus_tag="K8M77_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085039.1"
                     /translation="MESQENSKKSSVAKNKLEKIYSTFLHDGYRFTEFRIGDENNWAY
                     IQYSAGVFEDYQLAIRRIFKEENSLKGNIKNRCVFLENKNGERIGTIMIVPSLSEEDG
                     VQEIKYLALLPKYQDKGLGKLLISKAYKMVDDIEGATRINLEAYNDKSIVLFENLEF"
     gene            complement(11706..12545)
                     /locus_tag="K8M77_000512"
     CDS             complement(11706..12545)
                     /locus_tag="K8M77_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBI7085040.1"
                     /translation="MLYYKVVNNKYLHKDKEWIMMLHGIGGNLNIFKKQIDVLSENYN
                     LLLVDLHGHGNSQNYTLKNMKKTKGEISFKYICEDIVEILDKLNIEKVNVLGISLGTI
                     VGMQFEKYFPNRVSSMILGGGIVGMNLKSKFFLNLALVTKNIIPKEMFYKIVAYMSMP
                     CKNHKLSRNIFLNAAEKLDKNECFRWVELMREHLENYKYVKNNVKKIFIMGDQDHVFL
                     PTIKKFVPEEDIVVIKNCGHICNIDSFKTFNHLTLNFLINNHGYMFGAKERRKYVYKY
                     NFT"
     gene            complement(12679..12840)
                     /locus_tag="K8M77_000513"
                     /pseudo
     CDS             complement(12679..12840)
                     /locus_tag="K8M77_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907716.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12844..12993)
                     /locus_tag="K8M77_000514"
                     /pseudo
     CDS             complement(12844..12993)
                     /locus_tag="K8M77_000514"
                     /inference="COORDINATES: protein motif:HMM:NF013921.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13157..13999)
                     /locus_tag="K8M77_000515"
     CDS             complement(13157..13999)
                     /locus_tag="K8M77_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085041.1"
                     /translation="MNEISDLAKITSILGIAATTYLVYENNSIFINEYELFTRKTPNS
                     FRGFKILHLNNLYGKTFGSENYRLIEKINSLKPDIIVTTGNMLTSSLDKDFVFLELCK
                     NLRSFYPIYYSVEGCKNLSGKKYEKYRDYLKGLMDIGVILLNNSKVSLIKKEDKLNIY
                     GLYIEDEIKSFSFKEGFKKENIFSKEDVVNKLGKPNKSEFNIVLANDVINFEAYVKWG
                     SDITFSGYTRAINRYIFNNLMERKKGIFIEKDSSMILSRGLGNDFLKMRIMNRPEITL
                     ITIR"
     gene            14197..14739
                     /gene="lepB"
                     /locus_tag="K8M77_000516"
     CDS             14197..14739
                     /gene="lepB"
                     /locus_tag="K8M77_000516"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI7085042.1"
                     /translation="MNLLSESNISTLNKKSHLKIKNLILFSVIVFLCSYFLTNYLIFK
                     AYIPTGSMRPTIMEEDEVIVSKIYTSINRGDIFVFSHESSEELLIKRVVGLPGEKVEV
                     KDGLLYVNDVFIDEPYVKNNESMNKTFYVPEGNYLFFGDNRAISEDARRWENPYVPKK
                     NLDGKALFTVYPRDRIGLLK"
     gene            complement(14827..15354)
                     /locus_tag="K8M77_000517"
     CDS             complement(14827..15354)
                     /locus_tag="K8M77_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085043.1"
                     /translation="MVNEKFGILAKIFLLILILVPITFRVYDLYYLNSSWDINSKVVK
                     RGKVYRDYIKEKKLTGVPNIYYKYNSLTPEEMMTLDVLANTKGYNIEEVLPEVEKYVL
                     SKNNEKNGAKLNEYEKIELNKCVKKIKDGVNENLEKNSSYIYWMKMLMSVFTALMIVL
                     SIYAIYMTNVFINEK"
     gene            complement(15749..15904)
                     /locus_tag="K8M77_000518"
     CDS             complement(15749..15904)
                     /locus_tag="K8M77_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085044.1"
                     /translation="MPKDSQPDNKVARMEKCNFQTPITSEEGKNNNRTAKKHSVKREG
                     FQSSHIN"
     gene            complement(16133..16711)
                     /locus_tag="K8M77_000519"
     CDS             complement(16133..16711)
                     /locus_tag="K8M77_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085045.1"
                     /translation="MKKVSLILINIILVCNLIACGAQVNEIAINFIDNEIEVEENEKR
                     DITIQSKDKHKQFRNSNTKIKKEALKTYFYNDKTLKDFEVPKKTIEEYENWFINMARD
                     RGESAKDISDAFRSVDMLIGEDVVKFPVIIDTVNFNDKEAYAIAYLCEEGENLDMEAQ
                     EAMELKRFDEVLIIGVYKDSSDIFYKDKYSLK"
     gene            complement(17176..18459)
                     /locus_tag="K8M77_000520"
     CDS             complement(17176..18459)
                     /locus_tag="K8M77_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBI7085046.1"
                     /translation="MSLSTNELKEIVRKIGKDLSAKIEDKKLQELFYNCFINTMDTTV
                     EVSEGDAFVITGDIPAMWLRDSTSQVEHYLPFVKEYPELKAIFTGLINRQVKCIFIDP
                     YANAFNKEPNGQKWDNDITKDSPWVWERKYEIDSLCYPVRLIHKYWKESGDETFFNDD
                     IKKAFNMIIDLWRVEQYHREKSDYSFQRLNCSVTDTLSHEGLGTPVTYTGMTWSGFRP
                     SDDACEYGYLIPANMFAVVALRYISEIAEKVYKDEELKEKADGLREEIDNAIEKHGKV
                     YKEGFGEVYAYETDGMGNYNFMDDANVPSLLSIPYLEYKGIEDEVYQNTRKFILSKNN
                     RFFFEGKAAKGIGSPHTPDQYIWHIALSIQGLTTNNQEEIEELIKLLKETDAGTGYMH
                     EGFHVDDPTKFTRDWFAWSNSLFSHFIYEKVINKK"
     gene            complement(18904..19563)
                     /gene="trmB"
                     /locus_tag="K8M77_000521"
     CDS             complement(18904..19563)
                     /gene="trmB"
                     /locus_tag="K8M77_000521"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBI7085047.1"
                     /translation="MRMRRKPWARPELEACDFFVANPKENKGNWKNTFKNTENPIYLE
                     LGCGKGTFMAVHGSDHPNINYIAIDIKDEVLVLAKRNIEKAYEEKNKALDNVKLMPQE
                     IALIDTILDSNDKIERIYINFCNPWPKDRHKKRRLTHPRQLTKYRDFLVDGGEIHFKT
                     DDDELFEESLEYFKECNFEITYITRDLHNSGYEYNVVTEHEEMFSKQGIKIKFLIAKK
                     L"
     gene            complement(19740..22352)
                     /locus_tag="K8M77_000522"
     CDS             complement(19740..22352)
                     /locus_tag="K8M77_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBI7085048.1"
                     /translation="MINLNEEQQKVVNEQKNNIILLASAGTGKTNTLAERVTSIIKNG
                     NSKPSEILCITFTNKACKEMSDRVMKIVGGEAKDITIRTFHSFCFDVVRTYAKRNTDI
                     FSDFTIFDEDDCKEVISKLSYYYATSNLMATNMQIQRVIDFIKVERVRIETLEDRVLD
                     EYKTVIDEIFKFREEKINGVCTNKGNLNLNLKNYIKFHGHELVTLYDSKLREDHALDF
                     NDLIILTKELFKDEKVVRAIKNKFKYINIDEVQDTSIIEYSIIEKLFEGNNVLICGDF
                     FQTIYSWRGSDPEMIFNKFKEKYNPVEIVFSKNYRATKNLNKASLEFLNNAFSSKVST
                     MYKDGILAASKEDGEKILLKETRGLREEARFIFDYVNKLCKKGEDLKRVCVLTRDNNY
                     NIGISEELYQIGGYEGADFEFILVDQFKFFRRQEVKDILAFLKLIANRYDSLSLKRIV
                     NRLPTGIGMKTLEDIESYKYKEVGIRLADYIDPLVLEYGEKFSLLINEFEKENIIVFD
                     VESTGVDVTEDEIIQIAAIKLNKKGEVVDKFEKFLKNKKPVKDSYYVHGFSDEMLQRI
                     GEDKEKVLKEFVEYSKDSIIVGHNVQYDINILYSELERSNLGKPKFKTFYDTLDIYRR
                     FYPGNINYKLENLSKVYDTKHKPSHDAMDDIIATGELLVRAINEKIKETSMERMALMS
                     KHLKAFTAISKKLNELFQIAESKRPYELVACVINGFNIKSMYAGDENKEKLNRLRDLY
                     ALFRDLDDKSKGNRDALLDIIRITGLSNGELESLIINRTKKPRIPIITVHQAKGLEFD
                     TVFLAGIQNNTFPSYMAIKEGNLAEEKRVFYVAITRAKKRLVITYNSQGKYGHRNEIS
                     QFINYIPKEFFKII"
     gene            complement(22407..23150)
                     /locus_tag="K8M77_000523"
     CDS             complement(22407..23150)
                     /locus_tag="K8M77_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI7085049.1"
                     /translation="MKKILNVAHRGLSGLYPENTMIAFRKAIEADCDGIEMDVHLTSD
                     GVPVVIHDEEVDRTTNGSGYVKDFTYEQLCNLDAGIKFSDEFKGEKIPTLKEFFDFMK
                     DNDKLINIELKNSIIHYEGLEEKVYKEIENYGFEDRVIISSFDHYSIRKCIRLNKNIK
                     TGILYCDCIFEPHNYCKVVGSSALHPEFNSITEEIVKAAHEDFQQINVYTVNEKKDME
                     KMIDLKVDMIITNYPNILKEVLENKYNKV"
     gene            complement(23492..24283)
                     /locus_tag="K8M77_000524"
     CDS             complement(23492..24283)
                     /locus_tag="K8M77_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI7085050.1"
                     /translation="MKLLTLNCHSWLEEEQLYKIKYLAKTIVEEKYDVIALQEASQSI
                     KASIKEGNLKEDNYGLILIEEINKLGGEKYHLVWDYSHIGYDIYEEGLCILTKHKIVN
                     SESFYISKSHCKTFWRSRKIVKATIDINNENIDFYSCHMGWWHDKEESFTFQVENLLA
                     HAKESKNRAFFMGDFNNNAFLKNEGYDFLKSNGLIDTYDLAENKDSGVTVNKNIAGWE
                     DNTGELRIDIIFTNRNTKVYDSRVIFNGKNKEVVSDHFGLEIETT"
     gene            complement(24294..25949)
                     /locus_tag="K8M77_000525"
     CDS             complement(24294..25949)
                     /locus_tag="K8M77_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBI7085051.1"
                     /translation="MKKFKLGSFDFWQKFGKALLVVVAVMPAAGLMISIGKVMGIYID
                     VDFIKTIARVMEDIGWAIIGNLNLLFAVAIGGSWAKERAGGAFAGTIAFVLINRITGV
                     IFSVSNAMLASDSTATVQSFTGQTLLVKDYFVSVLGAPALNMGVFVGIISGFLGAALY
                     NKYYNYNKLPNALAFFNGKRFVPFMVIIGSTISAIILSFVWPFVQYGLNTFGQWIATS
                     KDTAPIIAPFLYGTLERLLLPFGLHHMLTVPVNYTELGGVYHILTGPTAGQVVAGQDP
                     LWLAWITDLNNFKQAGDITSYNQLIGSVVPARFKAGQVILSSASLIGVALAMYKNVDP
                     DKKKKYKSVFFSAAIAVFLTGVTEPIEFMFMFISPILYVVYAVIAGLGFAIADIINLR
                     VHAFGFIELVTRSPLMINAGLTKDLINFIIVAVVFFFLNYFVFSFLIKKFKIATPGRM
                     GNYIDNEDENANKASSNSKASMDELAVKAIELLGGKENIVDVDACMTRLRVTVKEIEK
                     VGDEKAWKDNKALGLIVKDKGVQAIYGPKADVLKSDIQDILGI"
     gene            complement(26113..27777)
                     /locus_tag="K8M77_000526"
     CDS             complement(26113..27777)
                     /locus_tag="K8M77_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBI7085052.1"
                     /translation="MNKKWWKELIAYQIYPKSFMDSNGDGIGDIQGIISKLDYLKDLG
                     IDLIWLCPMYKSPNHDNGYDISDYKDILDEFGTMDDFNELLNEVHNRGMKLIIDLVIN
                     HTSHEHPWFIESRSSRDNPKRDWYIWREGKGYEEPNNWESIFKGSAWEFCENSEEYYL
                     HLFAKEQPDLNWENKEVRRELYNMINWWLDKGIDGFRVDAISHIKKEEGLKDMDNPEG
                     LKYVSSFEKHMNVEGINSHLKELKEETFSKYDIVTVGEANGVSANEADHWVAEDEGTF
                     NMIFQFEHLNLWNYEEGQGFDVKAYKDVLTNWQNSLEGKGWNALFIENHDIPRVVSTW
                     GNDKEYLTECAKAFGAIYFLQKGTPFIYQGQELGMTNVKYHSICEYDDVKTINTYNER
                     IESGVSEEIALKEAWVTSRDNSRTPMQWNSSKNAGFTCGKPWIGVNENYKTINVEVEE
                     RDENSVLNFYKKLIKLKKSNEALIYGVYDLILEEDENIFAYTRTLNNDKFLIMANLTG
                     ENAKYVYEKEKLNSKDLILNNYEVCEHKNLTEFILKPYECRVYKLS"
     gene            complement(28089..29081)
                     /locus_tag="K8M77_000527"
     CDS             complement(28089..29081)
                     /locus_tag="K8M77_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7085053.1"
                     /translation="MKVTIKDVAREANVAPSTVSRVLSDSHKISEETKIKVNKAIKKL
                     NYKPNAIARSLVNNKTKILGVVLPNEADDSFKNPFFVEAMRGMSMSAEENEYYIMYAF
                     SKNDEDELKTIKEFSSNNLLEGLCLLSVREEDKTIKYLKDINFPFVVIGRPDEEDVLW
                     VDNDNFQAMFKLVDKFIKDGQSDIAFIGGKKSWNVSKDRLSGFKGAFKANNLNYDKTI
                     VIEGIDFSEECGYLAMREIISKKNPKIVVTTDDLLALGATKFLNEEGIEGVKIVGFNN
                     TPMTKYQNPPISSIDINARELGYEATNLLIDYLNGNLVGRKKYCIVDTQFINRG"
     gene            complement(29292..30098)
                     /locus_tag="K8M77_000528"
     CDS             complement(29292..30098)
                     /locus_tag="K8M77_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI7085054.1"
                     /translation="MYCSKCGKEIPEESVFCPECGSRCKLSADKKNKEIDTKEIKELI
                     INLIFSPINTMKNGNINLSSKNKIIYILITTLIMPLITVLFLRSSSFNLIKSIPSTIA
                     KFSGSSSWNINDSIEFKNQFNMIMQNLFPTGKIYFLSLSSYILNYALLLGIIYIVFKF
                     IIKDNFDKINILNILFIISTFNVLLKIISSIALILGLLPWVALKLFSVILMIVLVYSL
                     LNTFIESREKLIYVYPATLVIVYLINMKFIFSIVVFIMSDMFLNNFLNFM"
     gene            complement(30128..31705)
                     /locus_tag="K8M77_000529"
     CDS             complement(30128..31705)
                     /locus_tag="K8M77_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBI7085055.1"
                     /translation="MRKCKSCGRIIEGNDRFCLICGAKIEEVKEELNNNETDVKTYEK
                     EKLENSNEELKESTEKPLKCEKNIYDNREKNKKSPLKGRSNKLILISLSLFGILFFSY
                     KQLGAYMSNPQNVIEKFKVSILDKNEQKLIDLLDTESSSLKIDEEGAKVIIDYFNKNL
                     DALDETINYLIRDSKLINKNDELSNMLNLNTDNEKKLFYIKNSKKGFLGLNKIKVYIN
                     PIFINVNSKVEGVKFFINGKEMCESDKRDFNLKVGPLVPGEYELSYKFNNEYVSLEQT
                     ETVDLIENFSLYDNEFIYEMGNELKYVTLKTSEKSKEATVYIDGKNTGKKAYELSDIG
                     PILYNTKISIEIKQEDNIFKSDEVEVGKRKELYIDLDSQLYEYEIKNNKEEDKIGKVS
                     SNFDNDYSELYYNVENLVKDYTYNFTVAVNNNNFEAIKKYLYQGEDLYDSQESIVKQF
                     YNDGIREELIDIKIEKINILNEQELSGTVEAIERYSIKQNDEEQEKLFNVLYEFKFNN
                     ISGNYELTNIKHLDKLN"
     gene            complement(31877..33748)
                     /gene="htpG"
                     /locus_tag="K8M77_000530"
     CDS             complement(31877..33748)
                     /gene="htpG"
                     /locus_tag="K8M77_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBI7085056.1"
                     /translation="MEKREFKAESKRLLDIVINSIYTNREIFLRELISNASDAIDKVY
                     YKTLGDSSLTFNKDDYYIKIKPNKEERTLTISDKGIGMTEKELDENLGVIAKSGSLQF
                     KKENEIKDGFDIIGQFGVGFYSGFLVADKITVITKAFGADKAYKWESDGVDGYTISEA
                     EKDSFGTDIILHLKANDEDEIYDEFLEEYKLKSLIKKYSDFIRYPIKLDVTKSRVKEG
                     TENEHEEYVEEETVNSMVPLWRRNKNELTDDDYNNFYVEKRFGFDKPLRHMHISVDGM
                     ISYNSILYIPENIPYDYYTKEYEKGLELYSNGVLIMEKCSELLPDYFGFVKGIVDSQD
                     LSLNISREMLQHDRQLSRIAKNIKTKIKNELESMMKNDRESYEKFYKSFGRQLKYGVY
                     DDFGMNKDELKDLLMFYSSKEKKMVSLAEYVERMAEDQKYIYYAVGESNERIEKMPQT
                     EMVLDKGYEILYFTEDVDEFAIKMLMNYKEKEFKSVSSGDLGIESEEENKKENEENKG
                     IFEAMKEALGEKISAVKASSRLKNYPVCLSSEGEVSIEMEKILSAMPNNQGVKAQKVL
                     EVNTNHEVFNSLKDALENDRDKFNLYTKLLYNQALLVEGLSIEDPVDFTNDICKLMK"
     gene            complement(33924..34562)
                     /locus_tag="K8M77_000531"
     CDS             complement(33924..34562)
                     /locus_tag="K8M77_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBI7085057.1"
                     /translation="MNISYELNIYKIIWIFTIGSILGYCVEMLWCYIRNGHFESRKGL
                     IYGPISPVYGIGGVALTVLLYRLINYNGIIIFFITAIIGGVFEYLCSWFQEKLFSSVS
                     WEYSSKALSIHGRTSVEYSVFWGILGVFFLRDIYPLFDKFMGLFSFQTVKTITLIFLV
                     LLIPDIIISSLAVRREVARMKGIKAKNALDRFLDKKYTDEFLKKIYPNMIFK"
     gene            complement(34740..35300)
                     /locus_tag="K8M77_000532"
     CDS             complement(34740..35300)
                     /locus_tag="K8M77_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBI7085058.1"
                     /translation="MRRLEEILDFNKNFVKNKEYEKYKATKKPEKKLVILSCMDTRLT
                     ELLPKAMNIKNGDAKIIKNAGATIMHPFGSIVRSILVAIYEFNAEDVLIVGHHGCGMS
                     NLNSKDMIKKMESRGISEETILTIKHCGIDVEKWLHGFGCVEESVKKSVTSLKNHPLM
                     PSDVNVHGLVIDPQTGELNVIVNGYE"
BASE COUNT        12008 a         5989 c         3395 g        14142 t
ORIGIN      
        1 ttattatatt ttgatacagc ccctttcata taattcaatg ggcaaatcat ctggatcact
       61 aaagaaagta aatttacgtc ctgtaaattc atctattctt atttcttcca ctttaatacc
      121 tttttcctta agttctctaa cctgcttctc aatattttca acctcaaagg caagatgtct
      181 aagtccacag gcctctggat agcttggtct tttaggtgaa tttttaaatg aaaataattc
      241 tatttgtgaa tcccctattt ccaaatcaag cttataggaa tccctttcct ctctataaac
      301 ttctcttata atttttaaac ccaataagtt tacataaaaa tcctttgatt ttttataatc
      361 tgaagcaata attgccacat gatgtatctt ctttaaattc atttattttc cccctaaatt
      421 ccatgaataa ttaagatgtt ttttcataat accctaacaa attcttctcc aagctaaaat
      481 ttaaaataag gctaatttaa cttagaatca atattaatga gttccatgaa ctattttaaa
      541 gccctctttt tctaaaattt tctcatattt ttttagatca tcacatttag attctataca
      601 gtgagatata tgtattgttt ctacgccatt cttttttaat tgctttactt tatgtgtcca
      661 attctctcct tcaactaggg tgtccttgca accagcgcaa taaaagaaac tagctaaatc
      721 ttctttattt tctccataaa tactaaaagc cgctgttgaa ttattatatg ccttaaagca
      781 tcctgatgca gcacattttc ctattacttt tctacatgcc attattgcta tactcattaa
      841 aattattccg ttgccaaaaa tctataaata cctataaacc tttctataaa aagcatcata
      901 catatacaag ttatcttgtt tatcatatgc cttttattaa cattttatag atttataaca
      961 acggttacct ccattctatc tatttataca aaaaaattat atcataaatt attcctaaag
     1021 acttaaaaaa agcaacaaag tctaagcctt gttgcccata tattatgaaa tttaaagtgg
     1081 tcaatgtaat tatatttact ttaaattaaa tatatctgat tttttaaaac tcttttttat
     1141 tccctaataa ctacattgct tttttaaata tagctattac ttcttctaag tttccttttc
     1201 ttggatttgt taatgaacaa gcatcattta aagcattagt agctaaagtt tcaaaatcac
     1261 tttcttttac tcctaatact ccaagtccct ctggaattcc tatagtttgt gataatgatt
     1321 ttatcatatc tatgcaaaga tctgctcctt gctcatcagt taatccttct atgtttccac
     1381 ccattatttt agcaatttta gctaatcttg aagcacttgt agatttgtta tattcttgaa
     1441 catgaggtaa taatattgca ttacatactc catgaggtaa gttatagaat ccacctaatt
     1501 ggtgagccat agcgtgaaca tatcctaaac tagcattgtt aaatgccatt cctgctaagt
     1561 attcagcata agccatcata tctctagctt ccacatcttg tccattttca acagcattca
     1621 ctaaataatt gcttataagt tctatagctt tctctgcaca agcatctgtt attggtgtag
     1681 cagcagttga aacataagcc tctatagcgt gtgttaaggc atccatacca gtagccgctg
     1741 ttaatgattt tggcatagca agcattaaca taggatcatt aaccgctatt attggtgtta
     1801 aatgcttatc tactaaagcc atttttatat gtctttcttc atcagttata atagcgaata
     1861 aagtcatttc actaccagta ccagcagttg tatttatacc taccattggt aattgaggtt
     1921 ttttagattt atctactccc tcatagtctt ttatttctcc gccattagta gctaataaag
     1981 ctattccttt agcacagtca tgagctgagc ctccacctaa tgaaataaca aaatcacatt
     2041 ctttttcttt taataatgct aaaccatcat taacattttt aactgttggg tttggtttag
     2101 tttcatcata tattgcatat tctatattct tattatctaa aacatctgtt acttttttaa
     2161 ctattcctat ttgacctaaa actttatctg ttactattaa agctttttta aatcctaatt
     2221 ctccaagttg atcaccagcg tcttttaagc aatctgctcc cattaaactt attgctggca
     2281 taaaaaattt ataactcata tatttctcct ctaactcttt ctattaagta tattctcaat
     2341 atttactttc actttataat ttaattttaa ttcatcgtta aaaatttaac taatccattt
     2401 ttttaaaaaa tatctcattt ttaaatcgac agaaattgac aatactataa taagctttta
     2461 tattatttat tatgaggtga gaattttatg gatagatttg atagacaact tgaattggca
     2521 gatggtatag aaactgaata tttaagaatt ctttattaca cctttgagaa aaattataaa
     2581 gatacatata agtcttatga atataaaaga ctatgcacta tagttgatgg agaaaaaaat
     2641 gtaagaatta ataatggtaa gaatatcata tatgattcat ctaaatatat gcttcttcca
     2701 ccaaactcaa gcgtggaaat gaaaattgaa aaacctacta aagccttagt tttagaatta
     2761 aatgaagatt taataaatac agtttcagaa aaactatctt atgaattaga tgtagattta
     2821 aataaatcta tattagaaaa accatttttt aatgaaaata atgaattaat agaattgaca
     2881 actaaaaaaa ttgtagagac tgctactaaa gatacaaata ataaagaatt tttacttgat
     2941 ttatatgcac aagaaataac ttatagccta ttaaatacta agggagctaa agatattctt
     3001 tctagtgatt ttaataatcc aataaatcgt tctattcaca taatgaaaga aaatcttaaa
     3061 aataatataa cagtttcaga aattgcttat gacctaaata tgtctcttcc aagcttttct
     3121 tcgaaattta aaaaaattat gggaatgaca cctaacgatt atcttacaaa cttaaaactt
     3181 aatgaagctg caagacttct taagttttca tcagttacag aggtagctta tgatttagga
     3241 tatgaaaata tttctcactt tataaaatta tttaaaaaga agtttggatt aacccctaaa
     3301 caatattcct taaagtattc tggtgaagtc ataaagattc aataaaaaaa taaagggcta
     3361 tataaaaata tatatataca aaactcacaa acatattctt agaattatta atactctttt
     3421 cacatagttt gtaagttttt gtatttttta tatctgccct tatttttata gaatactaac
     3481 taaagtttaa ttacctctac tttatagcca tctggatctg ttacaaagta atatgttgca
     3541 gctccatctg aaagaccttt cataggtgta acctcatatc ctaaagaaat atgtctttct
     3601 ctttcagctt ctatatcttc tactccaaca gctatatgac taaacccatt tcctaaatca
     3661 tatggctttt cttgatcata gttataagtt aattccactt catatccatc ttctttatct
     3721 gaaagataaa ctaaagaaaa tttatattct ggataatccc ttcttcttgt ttcaataagt
     3781 cctaaagctt cctcataaaa ctttaaagat ttatctaaat cttttactct tatgcatgtg
     3841 tgtaacattt tgcttgccat ttcacaacct ccatatttta aatattactt tttgtttttt
     3901 tattatatat tatcatatgt atatttgcat atccctatgt aatttaaaat aacactatat
     3961 tcttataaaa caagtattat aattcttttc ttataaattt atttaaggaa aacttgtaaa
     4021 aaacaattaa tataaatatt gatattactg ctaatataag taccacaaga ggtaatgaat
     4081 ttaaaatatt tgaaacatca taatttatat ttttaagtaa tttttctact aaagcaaggc
     4141 cgattattaa tataaataat ggaacgcttc ttaaataaga tactacactg ctctctgggt
     4201 atataattgc catactaaat gttattgatg catatatata gaaaattata aatgttaaaa
     4261 gtaaattaaa tgtatttaca atgatttttt ctcctaacat aaaacttata cctatacctg
     4321 ctaatacatt tggtaaagaa aatattaaaa aaattaatat aggtaatata aaagatgtgc
     4381 ctactacact tcttgcatca attggcagtg atcttaataa tttatttgtc attgctcctt
     4441 tattagtatt ttttgcaatt ataaaattaa cactaatcat tgaagctaac aaggtaaaat
     4501 aaatgaaaac actatttatt aaaaaatcat atctatcttt cggtaagtct ataaaaaaag
     4561 gagaatatcc caaaatcatt gtaaggatat aaaatataaa tattactctt atatttttta
     4621 agtctttaat aattaaattt ttcatattaa ctcacctcac tttttttata aatatttaga
     4681 gacactatta atgatattat tagagccagt attccaaatg ctaaaccata aactccatta
     4741 gtaatattaa ataatacttg taaattttca ttttcaagag aattaaataa tattgaacta
     4801 atcatcatta ttaaaataat aaatattgta ttaaacactc tataaaattt atgacttata
     4861 atcatatata ctggtaaagt cagtgataca taaaatagtt gaactattat atttggaaga
     4921 attgctctta atgttaatgt aaaatcactt cctgaaatta ctcctaaaat agtacagaat
     4981 aaatataaaa ataaattaac tactataata taaattaatg ttgatagata tttactataa
     5041 atataatttg attttttagt tggtaaagaa tttaagtaaa tattaaattt actttttctt
     5101 tcaacttcaa ttggtattat cgtaagataa aacacaataa aagttcccat agtatataaa
     5161 taattcattc cttgagttaa cgcaaaagta atgcctaaaa aaagaaacac aataattgaa
     5221 ctcttgatat atttcttttg tataaaaaaa tctaacttaa gtaattcttt catactactt
     5281 cacatccttt ttagaataat aaacaactat atcatctaaa gtagcctttt caaaaataat
     5341 tttttcaccg aaaagttctt ttaaatttct catattgtta gttagccctt caaagttata
     5401 tcttgttttt cttaatccta ttaattcttt ttctaaatcc ttatttaaaa tatctaaagg
     5461 tcccttaact aaaaagtatt cttctaataa caagtctttt tctaaggaaa attcaacttt
     5521 tcctttgttt aaaaaggtta tataatcagc taatttttca agatccgttg ttatgtgagt
     5581 tgaataaagt attgatactt cttcctcacc tattatttca aataaaatgt ctaaaagttc
     5641 acttctaaaa acaggatcta aacctgatgt tggctcatct aatattaaaa gttctgcctt
     5701 atgagataaa gctaaggcta aagaaaatct cattttttgt ccctttgata attgttttag
     5761 ctttttattt tcatttaact caaatttttc aacataatac ttaaattttt cttcatccca
     5821 ttttgaataa aatttagata ttattttttt attttctttt attgtaaaat cttcaaaagc
     5881 acaacaatca tcataaacaa atccaattct atcttttatt gatatttcat ttttctttat
     5941 gtcttcacca aaaatatata tttctccact atctaaattt attaaattca ttatggcttt
     6001 tattgttgta gtctttcctg ctccatttgg ccctattaat cccattacaa aacctttagg
     6061 aatattaaag cttacatctt tcaaagaaaa tcctttataa tttttactta tattttttat
     6121 ttcaagtaca ttttccatat aaaccttcct ccttaaatct atcccttatc tttataattc
     6181 ctttaaagtt ttaagcattc caactaaatc attaaaattt agtcctatgc tatttgcaat
     6241 atcaattgct tctcctaatt tttcttctat ttctctaagc ttttcttcca ttaaaagttc
     6301 tttattttgc tctgccacat aagatccctt accaggtaaa gtgtatataa aaccatctct
     6361 ttcaagttct tcataagctc tcttggttgt gattacactt actctaagct cagaagctaa
     6421 gcttcttatt gacggcaatg ctgaattttc ctttaaatct ccagataaaa tctcattttt
     6481 tatagcttta gctatttgtt catatatggg tacccctgaa gtgtttgaaa ctactatatt
     6541 caaaaactca cctctttctg tgtatatact gtatatattc tatatataca gtatatacac
     6601 cattttcata tagtgtcaat atattttcat tatttaattt tttatcttta aaatcatctt
     6661 tacttaaaac cttataaaat aagagctacg tataaaaaat ccttttttaa tacatagcta
     6721 aaattaattt aaagttttta ctattaagat attggaatta aaggcaaaaa agaggtgcac
     6781 ataaatgtgc acctcttagg tttatataat tatttatttt cttctattaa ttcttcgatt
     6841 ttacatttgc atctagcacc acagcaagca ccagttcctg ctcctgtttt ttctcctact
     6901 tcttcaaaag aaagagctcc gttctttact gcctcaacta ttttttcttc tgaaacacct
     6961 ttacataaac aaacatttga cataataaaa ttctcctctc gttttacctg aatattatat
     7021 attacttatc tctaaattca tcattactat taattaataa tctttattgt ttaaatagat
     7081 aataacagat aaaaactctt cttgcacata ttaaattctt ttatttttaa tcactaaaag
     7141 ctattatatc aaattaaatc tatatttctt tattatgcta aggttttctc tttaaatact
     7201 tcaattccta ttctatcaat aaaatctcct aatttttctc cagcctcagc ttccccttta
     7261 taaacttcaa agattttatc aactattctt aaagcttcct tttcatctac atcctttata
     7321 agtatgtctg ccattcttgg attatgacct gctgaccctc ctgccgttac taaaaatcct
     7381 gttttatcag ctataactcc aacatccttt gaataaacac ttccacaggc atttctacag
     7441 cctgctactc ctattttagt tctacatggc atatccatat gcttatattt atttaataac
     7501 ttcattcctg ttcctatggt attaaacttt gatctcttac aaaaaccagc tggacacatt
     7561 tccacatttt ttactgagtt ttgttttttt acagcaggct ccattcctag ctcatcccat
     7621 attttaggca aatcttctcc cttaagattt gttattaaga gcctttgacc tgatgtaatc
     7681 ttaattactc cattatactt tttagttacc tttgcaattt tttctaattg ctctggagta
     7741 ataaatcctc ctggaatatg aggagttatt ccaaaagttc tttttccccc tctaactttt
     7801 tgtaagttcc cataatgagt tccgctaacc atcttaggta ttccaattcc tatactatcc
     7861 attctaaaaa attcctccta tatattaaat gtaaaaattt caatttataa attactctta
     7921 gtatacacta aaaatcattt ttcaaaagta acctaggcta cttttattaa tatctgttat
     7981 taataaaaag gttgtatcaa aatactctaa tgcactttga tacaaccttt tttatataac
     8041 tacatctatt ctaagtgaaa ttctattcac ttttattttt taattgttta atataacatc
     8101 tgaattgaaa ctagttgaat tatttaaata aatttttagc ttttcaattc tcttcttatt
     8161 tgttttacaa acttggaaag aaagattgtc atattcaacc atttgatttt cactttgcgg
     8221 aatatttcct attaagttaa caacaaaacc accaatagtc tctatatttt gactttcaag
     8281 atttaggttt aaataatcat ttaacttgtc taatgttaat aaaccatcaa ctacataggt
     8341 attattatct attttatcta tatagttttc tgaatcgtca tactcatcta atatatcacc
     8401 cataacttct tcaattaagt cttctatagt aacaattcca gaaaaaccac catattcatc
     8461 tattattaca gccatatgtt gcttactatc ttgaagttct ttaaataaat catctatagc
     8521 tttattttca ataacaaagt aagctggacg tattaattga tctaatttaa tgttatctac
     8581 tccaaccttt acaacggctt caaagaagtc ttttaaataa agtattccta ctatattatc
     8641 cctagtttca ctataaacag gaactcttga aaattgctca cttataagtt tcttagcaat
     8701 tgtttcattt ggagttgttg attttatagc aaagacttct cccctaggag tcataacttc
     8761 tttaacaact ttatgatcaa attcaaatat attatctaac atatcttttt cagctttatc
     8821 aattacacca ctctcttgtc caatacttat catggatttt atttcatcct tagatacagt
     8881 ttctttatct tcagacttat ttaaccctaa tatttttata aataagtttg ttgaagctga
     8941 aagaatcttt acaaatggtg ttgatatttt agaaataaca ttgattggtc ttatagccat
     9001 taaagcaatt tcttctggct tttgtaatgc aattctcttt ggaagtaatt ctccaaaaac
     9061 taaagttata taagacaaaa gcacagttat aagtataact gatatctcat taccataagg
     9121 aatatttagc ttgtacatga attgcgctag agtttctgaa atgctagtgg ctgctgatgc
     9181 actggcaaaa aatcctgcaa atgttattcc tatttgaata gtagataaaa agttaccagg
     9241 tgattttaaa acctttttta ataaaagggc ttttttgttc cctttctctg caagcatctt
     9301 tatcttagat ttatttacag ataccatcgc catctctgca gctgcaaaga acgcattaat
     9361 taagataaga actaatatta gaataatttt tggtaaaata ctgggactcg ggtccattct
     9421 ttaactcctc ctctaaaaaa ataaattttc ttctttatct tatagattaa tataaaatat
     9481 cgtataagat aaccaatttt aaaccataat accatactgg tatcttttat tcaattatat
     9541 aatatgcaca atttaaccta aattatacta aataaataat ttaagtcact tatattattt
     9601 attacatata tttgaaaaat aatactataa tatttaactt ttaagatctt ttctacatat
     9661 gataaagaag acaaatttaa gctttaatat taagtaaggt gatttattgt gaaagaaaat
     9721 ttgaatcttt ttaaatttct ttttggaaca actattcttt tgtgttgttt aagtaaagat
     9781 gtaatcttaa attccttaat atacacaaaa acaacattta aagctaaaat tcacaaaagt
     9841 aaataaaata agtttctgta agataattat attaactaat aataattatt gattttttta
     9901 tactatatag tataatttaa ttaaaagata tgaataattt attgggaggt atggattatg
     9961 agtttagcaa aattaataga cgaaaaaaga aaagctaaag ctagagctga aagaaataaa
    10021 aaagctaaag ttgctgtaac tggggctgcc gttggtacat tagcaggagt attaggtggt
    10081 ttactacttg ctcctaaatc tggaaaagaa acaagaaatg atataaaaga gggttcaaaa
    10141 aaggctgctc aaaaattaaa tgataaaagt gttgaattaa aagaaaattt aaatacacaa
    10201 gttgcaaaag gaaaagagaa tatttctgat gcaaaaagca aaataaaaga atatcttaat
    10261 agtaaaaaat gtaagaacat tgaagaggct gtagaagaaa acttaaatgt tcctgttgag
    10321 gttgcagatg aagctacttc tttagaaact gaagaagtta aagaatctaa ctaataatta
    10381 tgtatataga tttaaatttt atttacctat tattagcttt attaggttgt atagctattg
    10441 tttatttgat attaatatta aataaagttt taaaattagt atcaaatata aatagtcttc
    10501 tagatcaaaa taaggacaac cttactaaga ctattaagga tttacctaaa atagctgata
    10561 atgtaaatga tgcttgtcaa aacattaagg atatatctga tgttgcaact gaaactacag
    10621 ctgacttaat tgttgctaag gaaaatatag aatctaatgt tgaaacagtt aaagaaattg
    10681 ctaagataat taatcaagtt ttctttaaaa aataaaaaaa ctcattccaa aaaaaatctt
    10741 aaaagattta aattggaatg agttttttta attatcatgc tctttaaaat tctaaattct
    10801 caaaaagaac tatagattta tcattataag cttctaaatt aattctagta gccccttcta
    10861 tatcatcaac cattttatat gcctttgata taaggagttt gcctaacccc ttatcttgat
    10921 atttaggaag aagtgctaaa tatttaattt cttgaacccc atcttcctct gaaaggcttg
    10981 gaacaatcat tatagttcca attctttctc cattcttatt ttcaagaaat acgcaacgat
    11041 ttttaatatt tccttttaag ctattttcct ctttaaaaat ccttctaatt gctaattgat
    11101 aatcttcaaa aacacctgct gaatattgaa tatatgccca gttattctca tcaccaattc
    11161 taaattcagt aaatctatat ccatcatgca ggaaagtact ataaattttt tctagtttat
    11221 tctttgctac tgaagatttt ttactatttt cttgactctc cattttcatt cctccctata
    11281 aagctttgta ttaaggtttt atcatattcc tatttctatt tctattataa ttttacccaa
    11341 taatttcatt ttattagtta caacaatatt attttatata actatttata ctaaattata
    11401 gaattctttt tgtaatattt ctagtaactt attattcatt tccatccatt aatgcttata
    11461 ttagatatat atatccaata tatccactaa taatgtatta gataaaaaat aagccatatt
    11521 aaaaccctta aataaaagtt ttaatatgac tttaatactt ttctctatct taaactattt
    11581 tcaaaattac tctagatcta catttaacta aaaatcaaaa ttctcctaga acatagccta
    11641 atatttaata taatgttttc tctaaggctg tttaattttc tttctaaaat atcacagaat
    11701 atattttatg taaagttata cttataaaca tattttcttc tttcttttgc cccaaacata
    11761 tatccgtgat tatttattaa aaagtttaat gtaagatgat taaaagtttt aaaggaatca
    11821 atattgcata tatgcccaca attttttatt acaacaatat cttcttctgg tacaaacttt
    11881 tttatagtag gaagaaaaac atggtcttga tctcccataa taaagatttt ctttacatta
    11941 ttttttacat acttataatt ttctaaatgt tccctcataa gctcaaccca tctaaaacat
    12001 tcatttttat ctaacttttc agcagcattt aaaaatatat ttcttgaaag cttatgattt
    12061 ttacatggca ttgacatata agctacaatt ttataaaaca tttcctttgg aataatattt
    12121 tttgtaacta aggctaaatt caagaaaaat ttagacttta agttcatacc tacaattcct
    12181 ccccctaata tcatagaaga aactctatta gggaaatatt tttcaaattg cattccaact
    12241 atagtaccaa gggatatacc taatacgttt actttttcta tatttaactt atctaatatc
    12301 tctacaatat cttcacaaat atatttaaaa gatatttccc ctttagtttt tttcatgttt
    12361 ttcaaagtgt aattttgtga atttccatgg ccatgaaggt ctactaaaag caagttataa
    12421 ttttctgaca gtacatctat ttgcttttta aatatattta aatttccacc tattccatga
    12481 agcatcataa tccattcttt atctttatgg agatatttat tatttactac tttataatat
    12541 agcatttata ttacactcct taatactctg atgtgaacta ctcttcactt aataagtggg
    12601 agcttcttgg tcaatatatc taatgataca agtttaccca agcttacagg gtagttccta
    12661 ccccagtata atatctattt atactatcaa tttctctaag tttttagttg cattaatatc
    12721 tctatcgtgt tctgttccac atttcggaca agtccaattt ctaagtgcta gactttttac
    12781 atcaccattt ttatatccac attcagaaca tagttgtgaa ctagcataat ttgatggagc
    12841 aaaatgataa ttgtcatgat cccattcttc taaagttata ttatacttag atgaaatata
    12901 ctcaaatatt tctttaagtc tgttggatat atcatcatta attacttttc tacgatattt
    12961 tataactata ctacttatta taagaatatc tattgttgac taacgtcaat ggctatccat
    13021 ctcacactta tagaagatag gagaattctg tacgattggt taaattctat catcttttca
    13081 taatctatat cccactgtct atggatattt gaataaaatt ttacacagca ttaacattgt
    13141 ttaaacctaa gcaatattac cttattgtaa tcaatgttat ttctggtcta ttcataattc
    13201 tcatttttaa aaaatcattt ccaagccctc tacttaaaat catagatgaa tctttttcta
    13261 tgaaaatccc cttttttctc tccattaagt tattaaatat atatctattt atggctctag
    13321 tatatcctga aaaagtaatg tcacttcccc actttacata ggcctcaaaa tttattacat
    13381 catttgctag cacaatatta aattcacttt tattaggctt ccctaattta tttactacat
    13441 cttcttttga aaatatattt tccttcttaa atccttcttt aaaagaaaag gactttatct
    13501 catcttctat gtaaagtcca taaatattta atttatcttc cttttttata agtgaaacct
    13561 tactattatt taaaagtatc acccctatat ccattaaccc ctttaaataa tctctatatt
    13621 tttcatactt tttcccactt aaatttttgc atccttctac agaataataa atcggataaa
    13681 agctccttaa atttttgcat agttctaaaa aaacaaaatc cttatctaag gatgaagtta
    13741 acatatttcc agttgtaaca ataatatctg gttttaaaga atttattttt tctataagcc
    13801 tataattttc acttccaaag gtttttccat ataaattatt taaatgtaaa atcttaaatc
    13861 ctctaaagga attaggagtt tttctagtaa ataattcata ttcatttata aaaatactat
    13921 tattttcata gactaaataa gttgtagcag caattcctaa aattgaagtt atctttgcca
    13981 aatctgaaat ctcattcata ttaatactaa ccctttaaat ttttactctt atattttata
    14041 tatgaatata aaaatataat taaaactcct aaacctttta cttttatttc caaatattgg
    14101 tttttatggt attattattt taagatagga ggtgtccttg ttgcaaattt aattaaattt
    14161 aatgaaatat agtacctaag gcaacataat atatttatga atttattatc tgaaagcaat
    14221 atatctacat taaataaaaa atcacactta aaaataaaaa atttaatact cttttctgta
    14281 atagtttttt tatgctctta ttttttaacc aattacctaa ttttcaaagc ttatattcct
    14341 actggttcta tgagacctac aataatggaa gaagatgaag taattgtgag taaaatttac
    14401 actagtataa atagaggaga tatctttgta ttcagtcatg aatcctccga agaattgctt
    14461 ataaaaagag ttgttggtct tccaggcgaa aaagttgaag taaaagatgg gcttttatat
    14521 gtaaatgatg tttttattga tgaaccttat gtgaaaaata atgagtcaat gaataaaact
    14581 ttttatgttc ctgaaggaaa ttacctattt tttggtgata atagagcaat atcagaagat
    14641 gcaagaagat gggaaaatcc ctatgttcct aaaaaaaatt tagatgggaa agctctcttt
    14701 actgtatacc ctagagatag aattggactt ttaaaataaa tctctatttt aagctattgt
    14761 tgattttcaa ttaataaact tagttaaaaa tcaacaataa cttaaaaaat ctctaaaact
    14821 ttcatcttac ttttcattta taaaaacatt tgtcatataa attgcatata tgcttaaaac
    14881 tatcattaat gcagtaaata ctgacattag cattttcatc caatatatat aagatgaatt
    14941 tttttctaag ttttcattta ctccatcttt tatcttttta acacacttat ttaattctat
    15001 tttttcatat tcattaagtt tcgctccatt tttttcatta tttttggata aaacatattt
    15061 ttcaacttca ggcaaaactt cttctatgtt atatccctta gtatttgcta aaacatctaa
    15121 agtcatcatt tcttctggag ttaaagaatt atatttataa taaatgttag gaactcctgt
    15181 taatttcttt tccttaatat agtctctata taccttcccc cttttgacaa ctttagaatt
    15241 tatatcccaa cttgaattta aataataaag atcataaact ctaaaagtta taggtaccaa
    15301 tattaaaatt aataaaaata tttttgctag tatgccaaac ttttcattca ccataataaa
    15361 gcctccatta attttcaata tataacaaaa agtcctataa gctaagggat acttatagga
    15421 caaatgcata tatatatgaa atatagggaa attaggttta tgctttaacc tgttaatatt
    15481 atattaacat actgttactt attattcaag tattttttca aaaattaaac atttttttga
    15541 ttctaattcc tataaataaa ggaagttttt ttatttaagc tttcttttct atatttcaca
    15601 agtgaactac tctccactta tagaagtggg agcttcttgg tcaatatacc caagggtaca
    15661 agttttctca agctaacccc gtcgcaccaa gggtttatac aaaactaata taggactaaa
    15721 accatatttt aatatagaag attatatttt agtttatgtg agaactttga aatccttctc
    15781 tctttacaga atgttttttt gctgttctat tattattttt tccttcttca ctagttattg
    15841 gagtttgaaa gttacatttc tccattcttg ctaccttatt atctggttga ctatcctttg
    15901 gcataaatat attcctcctt agttagttaa attcaatttt atttttctca taaaaagaaa
    15961 atattattat ggtaaataat gaaataacac taatataaat attctctttt aaattataaa
    16021 attaagtttt tcatgtataa taaagttttt atatttaaat tattagtatt atatcttcat
    16081 aaaaatccac aaaataatta attttaagta gaatatattt ttcaaccaaa ttttatttta
    16141 aactatattt atctttataa aatatatcag aagaatcctt atatactcca attattaata
    16201 cttcatcaaa tctttttaat tccattgcct cctgagcttc catatctaag ttttctcctt
    16261 cttcacataa gtaagctatg gcataagctt ctttatcatt aaagttaaca gtgtctatta
    16321 taactgggaa tttaactaca tcctctccta ttaacatatc tactgaccta aaggcatcag
    16381 aaatatcctt agcactttca cctctatctc tagccatatt tataaaccaa ttttcatatt
    16441 cctctatggt cttctttgga acttcaaaat cttttaatgt tttatcatta taaaaataag
    16501 tttttaaagc ttctttcttt attttcgtat tactatttct aaactgttta tgcttatctt
    16561 tagattgtat tgttatatct cttttctcat tttcttcaac ttcaatttca ttatcaataa
    16621 aatttatggc aatttcattt acttgtgctc cacaagctat taagttacat actaagatta
    16681 tatttattaa aattaggctt acctttttca taaattcacc tcagtatatt tttacctttt
    16741 ttccaaacta tattcaaata aaaccctaaa caataaaact ctgaaaataa taaaaagctg
    16801 tataaaaaca actataaaac ttataaatat attccgataa ttactgaatt tcgctaagaa
    16861 tttatagcaa ttactccgaa gattttacta aagcgaaaaa ctcccttcgg tcaaacagtt
    16921 tcgcttctta acgtaaaatc ttctacgtaa tagcttaaat tctaaagctt attcagatat
    16981 tctcttcata ctatttataa gttttaatgt aagttatttt ttatacagcc tattaaaatc
    17041 tataaatata tatttaactt aattagagga ttattaagag taataaaatt ttatatttta
    17101 aacaacactg gagatagtat taaagagtat aactctttaa aataaccact aattaagtta
    17161 aaacaagcaa atttattatt ttttatttat aactttctca tagataaagt gtgagaataa
    17221 tgagtttgac catgcaaacc aatctcttgt aaacttagta ggatcatcta catggaaacc
    17281 ttcatgcata tatccagttc ctgcatcagt ttcttttaat aatttaatta attcttctat
    17341 ttcctcttga ttatttgttg ttaacccttg aattgataaa gctatgtgcc aaatatactg
    17401 atctggagta tgtggacttc caattccttt agcagctttt ccttcaaaaa agaatctatt
    17461 gtttttactt aatataaact ttctagtatt ttggtaaact tcatcttcaa ttcctttgta
    17521 ctctaagtaa ggtattgata aaaggcttgg tacattagca tcatccatga agttatagtt
    17581 gcccatacca tcagtttcat atgcataaac ttctccaaat ccttctttat aaacttttcc
    17641 atgtttttct atagcattat ctatttcttc tcttaaacca tcagcttttt ccttaagctc
    17701 ttcatcctta taaaccttct ctgcaatttc tgaaatatat cttaaggcaa ctacagcgaa
    17761 catgtttgct ggaattaagt atccatattc acaagcatca tcacttggtc taaatcctga
    17821 ccatgtcatt cctgtgtaag taactggagt tcctaatcct tcatggctta aagtgtcagt
    17881 tactgaacaa tttaatcttt ggaagctata atctgatttt tctctatgat actgctccac
    17941 tctccaaaga tctattatca tattgaaagc ttttttaata tcatcattaa agaaagtttc
    18001 atcaccagat tctttccaat acttatgtat taatctaact ggataacata gtgaatctat
    18061 ttcatacttt ctttcccaaa cccaaggact atccttagtt atatcattgt cccatttttg
    18121 tccattaggc tctttattaa aggcatttgc atatggatct atgaatatac attttacttg
    18181 tctatttatt aacccagtaa aaatagcctt taattctgga tattccttaa caaatggtaa
    18241 gtaatgctct acttgagaag tggaatctct aagccacatt gcaggaatat caccagtaat
    18301 tacaaaagca tctccctctg aaacctctac agttgtatcc attgtattta taaagcaatt
    18361 ataaaataat tcttgtaact tcttgtcctc tattttagct gataaatctt taccaatttt
    18421 tcttacaatc tcttttaatt cgttagttga taaactcatt tgctttaccc ccaagttcaa
    18481 atatttatta tttagttatt tcctaaatta aaactaaatt ttaattaaat atacttctta
    18541 agctataatt aaaacctaag taatttttta atcaacacta attaaaaact aaatttattt
    18601 aaattctatg ctttactatt taactaaaag ttgacttaat tttatagaat tcctaagcaa
    18661 cttcagatac attgtatacc atttcatcac tgtaataaat atactttttt aaagttcttt
    18721 atgtttctaa aatttatcac ttaaatctag aaactagaca agttcagaat aaacaactta
    18781 taaattacta ataaatattc ataaaaaaca gtagatagtt ttgtttttac acaaaactat
    18841 ctactgtgct aaatattttg agtctcatat ttaattctta tgctctcata ttttcaagta
    18901 aaattataac tttttagcta ttaaaaactt tatttttatt ccttgttttg agaacatttc
    18961 ttcatgctct gttactacat tgtattcata tccactgtta tgtagatcac gagttatgta
    19021 agttatctca aaattacact ctttaaaata ctctaaagat tcttcaaaaa gctcatcatc
    19081 atcagtttta aaatgaattt ctccaccatc aactaagaaa tctctatatt ttgttaactg
    19141 tcttgggtga gttaatcttc tcttcttgtg tctatcttta ggccatggat tacagaagtt
    19201 tatataaatt ctttctatct tgtcattgct atctaatata gtatctatta aagcaatctc
    19261 ttgaggcatt aatttaacat tatctaaggc tttatttttc tcctcatatg ctttttctat
    19321 atttctttta gctaaaacaa gaacttcatc ttttatatct attgctatat agtttatgtt
    19381 tggatggtca gaaccatgta cagccataaa tgttcctttt ccacatccta attctaaata
    19441 tattgggttt tctgtatttt taaaagtatt tttccaatta cctttattct cttttggatt
    19501 agctacaaag aagtcacatg cttctaattc tggtctagcc caaggctttc ttctcattct
    19561 catcttaaaa tccactccat cctttagttt cagaattatt atacagattt atatgaagta
    19621 tatcaacctt aggcttttcc taatcttcta aaatttatat ataatatcta attacctctt
    19681 aaaataatct ttcatctaaa aaaatcaaca aacctttaaa caaaagtcta ttataaaaat
    19741 tatataattt taaaaaactc ctttggaata taatttataa actgacttat ttcatttcta
    19801 tgtccatatt ttccttgaga attataagtt ataactaacc ttttctttgc acgagttatt
    19861 gccacataaa atactctttt ttcttccgct aaatttccct ctttaatagc catataagaa
    19921 ggaaatgtat tattttgaat acctgctaaa aatacagtat caaactctaa gccttttgct
    19981 tgatgcacag ttattattgg tatcctaggc tttttagttc tatttattat taaagattct
    20041 aactctccat tagaaagacc tgttattctt ataatatcta acaaggcatc tctattccct
    20101 ttactcttat catctaaatc tctaaataaa gcatatagat ctcttaatct atttaacttt
    20161 tctttatttt catctccagc atacatactc ttaatattaa aaccattaat tacacaggca
    20221 actaattcgt aaggtctttt gctttctgct atttgaaaaa gttcatttaa ctttttacta
    20281 atagccgtaa atgcttttaa atgttttgac attaaagcca ttctttccat tgaagtttcc
    20341 tttatctttt cattgattgc cctaactaga agctcccctg tagcaataat atcatccata
    20401 gcatcatggc ttggcttatg tttagtgtca taaaccttac ttagattttc taacttataa
    20461 tttatatttc ccggataaaa tcttctatat atatctaaag tatcataaaa agttttaaac
    20521 ttaggctttc ctaaattact tctttcaagt tcactatata ggatatttat atcatattga
    20581 acattgtgcc ctactattat agaatcctta gaatactcca caaattcctt taaaactttt
    20641 tctttgtcct ctccaattct ttgaagcatt tcatctgaaa aaccatgaac ataataggaa
    20701 tctttaactg gtttcttatt ctttagaaac ttttcaaact tatctactac ttctcccttt
    20761 ttatttaatt ttatggctgc tatttgaatt atctcatcct ctgtaacatc aacccctgta
    20821 ctttccacat caaaaactat tatattttct ttttcaaatt catttattag tagggaaaat
    20881 ttttctccat attctaatac aagaggatct atataatctg ctaatcttat accaacctct
    20941 ttgtacttat aagattctat atcctctaag gttttcatac caattcctgt tggaagtcta
    21001 ttgactattc tctttaaact taaagaatca tatctattag ctattagttt taaaaaggct
    21061 aatatatctt taacttcttg tctcctaaaa aacttaaatt gatctacaag tataaactca
    21121 aaatctgctc cctcatatcc accgatttga taaagctctt cactaattcc tatgttatag
    21181 ttattatccc ttgtaagaac acatactctt tttaaatctt cacccttttt gcatagcttg
    21241 tttacataat caaagataaa cctagcctct tctcttaatc ctctagtttc ctttaataat
    21301 attttctcac catcttcttt tgatgctgct aaaataccat ctttatacat agtagaaact
    21361 ttactagaaa aagcattgtt caaaaattct aaggaagctt tatttaaatt tttggtggct
    21421 ctataattct tagaaaaaac aatttctaca ggattatatt tctctttaaa tttattaaaa
    21481 atcatttctg ggtcagatcc acgccaacta tatatggttt gaaagaaatc tccacatata
    21541 agaacattat ttccttcaaa aagcttctca attattgaat actctataat gcttgtatcc
    21601 tgaacctcat ctatatttat atatttaaat ttatttttaa tggctctaac aactttttca
    21661 tccttaaata attccttagt aagtattatc aaatcattaa agtctaaagc atgatcctct
    21721 ctaagcttag aatcatatag tgttactaat tcatgaccat gaaatttaat ataattttta
    21781 aggtttaaat ttaaatttcc tttgttagta caaactccat ttattttttc ttctctaaat
    21841 ttaaaaattt catcaattac agttttatat tcatctaaaa ctctatcctc taaagtttct
    21901 attcttactc tttcaacctt tataaaatca ataacccttt gaatttgcat attagtagcc
    21961 attaaattgc ttgtagcata gtaataactt aatttactta taacttcctt acaatcatct
    22021 tcatcaaata ttgtgaaatc tgaaaaaata tctgtatttc tctttgcata agttctaact
    22081 acatcaaagc aaaaactatg aaaagttctt atggttatat ccttagcctc tcccccaact
    22141 attttcatta ccctatcact catctcttta catgccttat tagtaaaagt gatacataaa
    22201 atttctgaag gctttgaatt tccatttttt attatggaag taactctctc tgctaaggta
    22261 ttagtttttc cagttccagc agaggcaagc aaaataatat tatttttttg ttcattcaca
    22321 accttctgtt gctcttcatt taaattaatc ataatagcct tccttattta aaattttatt
    22381 tcttaaaaat aaataaaaat aaattattaa accttattat atttattttc taaaacttct
    22441 tttaaaatat taggataatt agttattatc atatcaactt ttaaatctat cattttttcc
    22501 atatcttttt tctcatttac tgtgtacaca tttatttgtt gaaaatcttc atgggcagcc
    22561 ttaactattt cttctgttat actattaaat tcaggatgaa gtgctgaaga cccaactacc
    22621 ttacaataat tatgtggttc aaaaatacaa tcacaataaa gtattcctgt ttttatattt
    22681 ttattaagtc ttatacattt tcttattgaa taatgatcaa aggatgatat tataactcta
    22741 tcctcaaatc cataattctc tatttcttta tatacctttt cttctaaacc ttcataatgt
    22801 atgatgctat ttttaagctc tatatttata agtttgtcat tatccttcat aaaatcaaaa
    22861 aactctttta gtgtaggtat tttttctccc ttaaattcat cagaaaattt tattccagca
    22921 tctaagttgc ataattgttc ataagtaaag tcctttacat atccacttcc attagttgtt
    22981 ctatcaacct cttcatcatg aattacaact ggaactccat cacttgtaag atgaacatcc
    23041 atttctatac catcacaatc tgcttctatt gctttcctaa aagctatcat agtattctct
    23101 ggatacaatc cacttaatcc tctatgtgct acgtttaata ttttcttcat agttattcct
    23161 ttcctttctt ttatattttt atttcttatt tttaaactta attccataac tacatattaa
    23221 atatttagtt acaatttcat tataacacct gtgaaaacat ttactgaaat taaaatttat
    23281 ttatattcta aaaaaggtac tattttcttt aaattttact tttaaaatta tatgggctgt
    23341 atcaaaatat aataataaaa cttataaaca tattccgaga atcactagat ttcgctaaga
    23401 attaaaaatt gttcctctta aaaacaaaga tagtacaagt aacttttatt acttgtacta
    23461 tctttcttaa aactattttt aatataaatt actaagtagt ttctatttct aatccaaaat
    23521 gatctgatac aacttcttta ttttttccat taaatataac cctactatca taaacttttg
    23581 tgttcctatt agtaaaaatt atatctattc ttaattctcc agtgttatct tcccatccag
    23641 ctatattttt attaactgta acaccagaat ccttattttc tgctaaatca taagtatcaa
    23701 ttaacccatt actcttaaga aaatcatatc cttcattctt taaaaaggca ttattattga
    23761 aatcccccat aaagaaggct ctatttttgc tttccttagc atgtgcaagt aagttttcaa
    23821 cctgaaatgt gaagctttcc tctttatcat gccaccatcc catgtggcaa gaataaaaat
    23881 ctatattttc attgttaata tctattgttg cttttactat ttttcttgat ctccaaaaag
    23941 ttttacagtg actttttgat atataaaagc tttcactatt tactatttta tgcttagtta
    24001 atatgcacaa accttcctca tatatatcat atccaatatg agagtaatcc caaactaggt
    24061 ggtatttttc tccacctagt ttgtttattt cttcaattaa tattaatcca taattatctt
    24121 cttttaaatt tccttcttta atactagcct taatactttg actagcctct tgaagagcta
    24181 taacatcata tttctcttca actattgttt ttgctaagta ttttattttg tataattgtt
    24241 cttcttctag ccaagaatga cagtttaaag taagtaattt catttttaat ctcctaaata
    24301 cctaagatat cttgaatatc tgattttaat acatctgctt taggaccata tatagcttga
    24361 acccctttat ctttaactat aagacctaag gctttattat ctttccaagc tttttcatct
    24421 ccaacctttt caatctcttt aacagtaaca cgaagacgag tcatacaagc gtctacatct
    24481 actatgtttt cttttccacc tagtaattca atagctttaa ctgctaactc atccattgaa
    24541 gccttgctat ttgatgaagc tttatttgca ttttcatctt cattatcaat atagttaccc
    24601 atacgtcctg gtgtagctat tttgaatttc ttaattagga agctaaatac aaagtaattt
    24661 aagaagaaga atactactgc aactattata aagtttatta aatccttagt taagcctgca
    24721 tttatcataa gtggtgaacg agttacaagc tctatgaatc caaaagcatg aacccttaag
    24781 tttattatat cagctatggc aaatcctaaa cctgcaatta ctgcataaac tacatataat
    24841 atgggtgaaa taaacataaa cataaattca attggttcag ttacaccagt taagaatact
    24901 gctatggctg ctgagaaaaa tactgattta tattttttct tcttatctgg atcaacattt
    24961 ttatacatag ctaatgctac ccctattaat gaagcacttg aaagtattac ttgtccagct
    25021 ttgaaacgag caggtaccac tgaccctata agttgattgt atgaggttat atcgcctgct
    25081 tgcttaaagt tatttaaatc agttatccaa gcaagccata atggatcttg tcctgcaact
    25141 acttgtcctg ctgttggccc tgtaagtata tgataaactc caccaagctc tgtatagttt
    25201 actggaactg taagcatatg gtgtaatcca aatggtaata ataaacgctc taatgtacca
    25261 tataagaatg gtgctattat tggagctgta tccttagatg tagctatcca ttgaccaaaa
    25321 gtatttaatc cgtattgtac aaatggccat acaaaagaaa gtattattgc tgaaattgtt
    25381 gaacctatta taaccatgaa aggtacaaaa cgtttaccat tgaagaatgc taatgcatta
    25441 ggtaatttat tataattata gtacttatta tataaagctg ctcctaagaa acctgaaata
    25501 ataccaacaa atacccccat attaagtgct ggagcaccta acactgaaac aaagtaatct
    25561 ttaactaaaa gtgtttgccc tgtaaaggat tgaactgtag cagttgagtc actagctaac
    25621 atagcattgc ttacactaaa tattacacca gtgattctat taataagaac aaatgctatg
    25681 gtacctgcaa aagctccccc agcacgttct ttagcccaag atcctcctat tgcaacggca
    25741 aataataagt ttaggttacc aattattgcc caacctatat cctccataac tctcgctata
    25801 gtttttatga aatctacatc aatataaatt cccattactt tacctattga aatcataagt
    25861 ccggcagcag gcattacagc aacaactact aataatgcct taccaaactt ttgccagaaa
    25921 tcaaaagacc ctaatttaaa tttcttcatt ctcaaatccc tccatttatt attcttataa
    25981 catttattct aaggttattg gttatataaa ctccaaatat taaatttttt ctttatatag
    26041 atctagctaa aatacaaaac taaaaatcaa ccttaagtta aaattagtat aaataaaaat
    26101 ataataatct ctttaggaaa gcttatatac tctgcattca taaggtttta atataaactc
    26161 tgttaagttt ttatgttcac aaacctcata attgttaaga attaaatcct tagaattaag
    26221 tttttctttc tcatacacat acttggcatt ttctccagtt aaattagcca ttatcaagaa
    26281 tttatcatta tttaaagttc ttgtgtaagc aaatatattt tcatcctctt caaggattaa
    26341 atcatataca ccatagatta aagcttcatt agacttttta agttttataa gctttttata
    26401 gaagtttaaa actgaatttt catccctttc ttcaacttct acatttattg ttttataatt
    26461 ttcattaact cctatccaag gttttccaca agtaaatcct gcattcttac ttgagttcca
    26521 ttgcataggt gttcttgaat tatctcttga agttacccaa gcttctttta atgctatttc
    26581 ctctgaaaca ccactttcaa ttctttcatt gtaagtattt atagttttaa catcatcata
    26641 ctcacatatg ctatgatatt taacatttgt cataccaagt tcttgccctt gatatatgaa
    26701 aggggttccc ttttgtaaga aataaattgc tccaaaagct tttgcacatt cagttaaata
    26761 ttccttgtca tttccccaag ttgaaacaac tctaggtata tcatgatttt caataaaaag
    26821 tgcattccat cctttgcctt ctaaagaatt ttgccaattt gttaaaacat ctttgtatgc
    26881 cttcacatca aatccttgtc cctcttcata attccaaaga ttaagatgct caaattggaa
    26941 tatcatatta aatgttccct catcttcagc tacccagtga tcagcttcat tagcactaac
    27001 tccatttgct tctccaacgg taactatatc gtactttgaa aaagtttctt cttttagttc
    27061 cttaagatga gaatttattc cctctacatt catatgtttt tcaaaggatg aaacatattt
    27121 aagcccctct ggattatcca tatccttaag accttcttct ttttttatgt gacttatagc
    27181 atcaactcta aatccatcaa tacccttatc aagccaccag tttatcatat tatatagttc
    27241 tcttcttacc tctttattct cccagtttaa atctggttgc tctttagcaa ataaatgtag
    27301 gtaatactct tcactattct cacagaattc ccaagctgaa cctttaaata tgctctccca
    27361 gttatttggt tcctcatacc ctttaccttc tctccaaata taccaatctc ttttaggatt
    27421 atccctagaa gatcttgatt ctatgaacca tggatgttca tgactagtat gatttattac
    27481 taaatctata ataagcttca tccctctatt atgaacctca ttaagcaatt cattaaaatc
    27541 atccatagtt ccaaattcat ctaagatatc tttataatca ctaatgtcat aaccattatc
    27601 atgatttggt gatttataca ttggacatag ccatattaaa tcaataccta aatcttttaa
    27661 ataatctaat ttagatatta taccttgaat atcacctata ccatcaccat tagaatccat
    27721 aaagctctta ggataaatct ggtaagcaat tagttctttc caccactttt tattcatatt
    27781 ttctccttgt gtttacgtat attgattgaa ttttaaattc gaactatgtg caaacgtttg
    27841 cccacaattc aaaaaaattt gctttttaga atcaactaaa aaacagattt taaaataagg
    27901 ctaaaattca aagttcgcaa agctcctttg aattaagtct cattttatat ttttataata
    27961 tcatattatt aaatccttta caaaattgat ttaatagaaa atttatatta ttttctctat
    28021 ttatcttact tttcctctta tatccttcaa ttcatgtgca aacgtttgcc caaactccat
    28081 gaggaattct atcctctatt aataaattga gtatctacta tacaatattt ttttctacct
    28141 actaaatttc catttaaata atctataagt aaattagttg cctcataacc taattctctt
    28201 gcattaatat ctatagatga aattggtgga ttttgatatt tagtcatagg tgtattatta
    28261 aaacctacta tttttactcc ttcaatgccc tcttcattta aaaatttagt tgctcctaaa
    28321 gcaagtaaat catcagttgt aacaactatc ttaggatttt tttttgatat tatctccctc
    28381 atagccaaat atccacattc ttctgaaaaa tctatcccct caataacaat agttttatca
    28441 taatttaaat tatttgcctt aaaagcacct ttaaatccac ttaatctatc tttagaaaca
    28501 ttccaacttt tctttccccc aataaaagct atatcacttt gtccatcttt tataaattta
    28561 tcaactaatt taaacattgc ttgaaaatta tcattatcaa cccataaaac atcctcttca
    28621 tctggtcttc caataactac aaaagggaaa tttatgtctt ttaaatattt tatagtctta
    28681 tcttcttctc taacagatag taagcataat ccttctaata gattattact tgaaaattcc
    28741 tttatagtct ttagttcgtc ctcatcattt ttgctaaaag catacatgat atagtattca
    28801 ttttcctctg ctgacatact cattcctctc atagcttcta cgaaaaaagg atttttaaat
    28861 gaatcatctg cctcattagg taaaactacc cctaatattt ttgttttatt attaacaaga
    28921 cttctagcta ttgcatttgg tttataattt aattttttta tagccttatt tacttttatt
    28981 ttagtttctt cactgatttt atggctatca gataaaactc ttgaaacagt tgaaggggct
    29041 acattagctt ctctagccac gtctttaatt gtaactttca caaaatcact ccttcacact
    29101 ttaaaatata ccataaaatc ctcctacaat ctacaaagtg atttaattaa gaaataccct
    29161 agcttagcta gtggtatttc ttaattctat tacaatttat tttttatgaa taatataaac
    29221 tttctttcct tgtataagtg atgaattctt gttgaatttc tatttatact taattattta
    29281 attgaattaa tctacataaa attcaaaaaa ttatttaaaa acatatcaga cattataaat
    29341 actacgatac taaaaataaa tttcatattt attaaataaa caataaccaa agttgctgga
    29401 tatacatata taagtttttc tcttgattct ataaaagtat ttagtaaact atacaccaat
    29461 actatcatta atattacact aaataatttt aatgctaccc atggtaataa tcctaaaatt
    29521 aaagctattg atgatattat ctttaataat acattaaatg ttgaaattat aaataatata
    29581 tttaatatat ttattttatc aaaattatct tttataataa atttaaaaac aatataaatt
    29641 attcctaata aaagtgcata attaagtata taactactta aacttaaaaa ataaattttt
    29701 cctgtaggaa ataagttttg cataatcata ttaaattgat ttttaaattc aatagaatca
    29761 ttaatattcc aagaagatga tccagaaaat ttagctattg tgctaggtat tgatttaatc
    29821 aaattaaaac tacttgatct taagaataaa actgttatta gtggcataat taatgttgtt
    29881 attaaaatat atattatttt atttttagat gataaattaa tatttccatt tttcattgtg
    29941 tttattggac taaaaattaa atttattata agttctttaa tttcttttgt atctatttct
    30001 ttattcttct tatccgcact taatttacat ctactcccac actcaggaca aaaaactgat
    30061 tcttctggaa tctccttacc acattttgaa caatacataa aagccccctt atttttatga
    30121 aatataccta atttaattta tctaaatgtt ttatattagt taattcataa tttccactta
    30181 tgttattaaa tttaaattca tagagaacat taaataactt ttcttgttct tcatcatttt
    30241 gttttatact atatctttca attgcctcaa cagtaccact taactcttgt tcatttaata
    30301 tattaatttt ctcaattttt atatctatta actcttctct tataccatca ttataaaatt
    30361 gctttactat actctcctgt gaatcatata aatcttctcc ttggtacaaa tattttttaa
    30421 tagcttcgaa attattgtta ttaactgcaa ctgtaaagtt ataggtgtaa tctttcacta
    30481 aattttcaac attataatat aattcactat aatcattatc aaagttgctt gaaacttttc
    30541 caattttatc ttcttctttg ttatttttta tttcatattc atataattga ctatccaaat
    30601 caatatataa ttctttcctt tttcctactt caacctcatc agatttaaat atattatctt
    30661 cttgtttaat ctcaatacta atctttgtat tatataaaat tggtcctata tcacttaatt
    30721 cataagcttt tttcccagta ttttttccat caatatatac tgtagcttct tttgattttt
    30781 cacttgtttt taaagtgaca tacttaagct catttcccat ttcatatata aattcattgt
    30841 cataaaggct aaagttttct attaaatcaa ctgtttcagt ttgctctaag ctaacatatt
    30901 cattattaaa cttataggat aactcatatt ctcctggaac caaaggacca acctttagat
    30961 taaaatccct tttatctgat tcacacattt ccttaccatt tataaaaaat ttaactccct
    31021 caacctttga atttacattt ataaatatag gatttatata aaccttaatt ttatttaatc
    31081 ccaagaaacc tttttttgaa tttttaatat aaaacaattt tttttcatta tcagtattta
    31141 aattaagcat attacttaat tcatcatttt tattaattaa tttagaatct ctaattaagt
    31201 aatttattgt ttcatctaaa gcatctagat tcttattaaa ataatcaata ataacttttg
    31261 caccttcctc atctattttt agagatgagc tttctgtatc taataaatca attaattttt
    31321 gctcattttt atctaatata gatactttaa atttttcaat aacattttga ggattactca
    31381 tataagctcc taattgctta tatgaaaaaa ataaaatccc aaataaactt aaagaaatta
    31441 aaattaattt attactccta ccttttagag gtgatttttt gtttttttct ctattatcat
    31501 aaatattctt ttcacatttc aaaggtttct ctgtagactc ttttaattct tcatttgaat
    31561 tttctaattt ttctttttca taagttttaa catcagtttc attattattt aactcttctt
    31621 taacttcctc tatttttgct ccacagatta agcaaaaacg gtcattccct tcaataattc
    31681 ttccacaaga tttacatttt cgcaaaaaat cccccccact atttaaataa taaatctata
    31741 cacactttaa tattttatta gatcgtatac ataattatta cttttaaaaa caataatatt
    31801 tttactctat atactttatt attagtgttt ttttcaacaa aaaaagctac atcattattt
    31861 gatgtagctt ttaaaattat ttcattagtt tacatatatc atttgtgaag tctacaggat
    31921 cttcaatgct taatccttca acaagtaaag cttggttata aagtaatttt gtatataggt
    31981 taaatttatc cctatcattt tcaagggcat cctttaatga gttaaatact tcatgatttg
    32041 tattaacttc taatactttt tgtgctttaa ctccttggtt attaggcata gcacttaata
    32101 ttttttccat ttctattgaa acttcacctt ctgaagataa acatactgga taattcttta
    32161 gtctacttga agcttttact gcacttattt tttctcctaa agcttccttc atagcttcaa
    32221 atataccctt gttttcttca ttttcctttt tgttttcttc ttctgattct attcctaaat
    32281 caccactaga tactgattta aattcttttt ctttgtagtt cataagcatt tttattgcaa
    32341 attcatctac atcctctgtg aaataaagaa tttcataacc tttgtctaat accatttcag
    32401 tttgtggcat tttctctatt ctttcatttg attcaccaac agcataataa atatattttt
    32461 ggtcttcagc cattctttca acatattcag ctaggcttac cattttcttt tctttagatg
    32521 aatagaacat taataaatct tttaattcat ctttattcat accaaaatca tcataaactc
    32581 cgtattttaa ctgtctacca aatgatttat agaatttttc ataactttct ctatcatttt
    32641 tcatcataga ctctaattca tttttgattt tagtttttat atttttagca atacgactta
    32701 attgtctatc atgttgaagc atttctctag aaatattaag tgataaatct tgagaatcta
    32761 ctataccttt tacaaatcca aagtaatctg gtaaaagctc tgagcatttt tccattatta
    32821 aaacaccatt tgaatataat tctaatcctt tttcatattc cttagtgtaa taatcatatg
    32881 gtatattttc tggaatataa agaattgaat tatagcttat cataccatca acacttatgt
    32941 gcatatgtct taaaggttta tcaaatccaa atcttttttc cacataaaaa ttattatagt
    33001 catcatcagt aagttcattt ttatttcttc tccataaagg aaccatacta tttactgttt
    33061 cttcttctac atattcttca tgctcatttt ctgttccctc tttaactctt gatttagtaa
    33121 catcaagttt aattgggtat cttataaaat ctgaatattt cttaattaat gattttaatt
    33181 tatattcttc taagaactca tcataaattt catcctcatc atttgctttt aggtgtaaaa
    33241 ttatatcagt tccaaaagaa tctttctcag cttctgaaat tgtgtatcca tccactccgt
    33301 ctgattccca cttatatgct ttatctgctc caaaagcttt tgttattact gttatcttat
    33361 ctgcaactaa gaatcctgaa tagaatccaa ctccaaattg tcctataata tcaaagccat
    33421 ctttgatttc attttctttt ttaaactgta aagaaccact tttagctata acccctagat
    33481 tctcatcaag ttccttttct gtcataccaa tacctttatc tgatattgta agggttcttt
    33541 cttctttatt tggttttatt tttatataat aatcatcttt attgaaagtt aatgaactat
    33601 ctcccaaagt tttataataa accttatcta ttgcatcact agcatttgaa attaattctc
    33661 ttaaaaatat ttccctgttt gtataaatag agttaattac aatgtctaat agtctttttg
    33721 attctgcttt aaattctctt ttttccattt tgaattattc cctcccaaaa caaaaaactc
    33781 atttattagc actctattaa ctcgagtgct aatctctatt tttatatata acactttttg
    33841 aaattaatgt caatatttaa tccaaaatgt acttatcaaa atataccaat aaaaaacatg
    33901 ccattctcct ataaatttta ttattactta aatatcatat taggatatat ttttttcaaa
    33961 aattcatctg tatatttctt atcaagaaac ctatctaatg catttttagc ttttatcccc
    34021 ttcatccttg ctacttctct ccttacagct aaagatgata ttattatatc tggtattaaa
    34081 agtactaaaa atattaatgt gattgttttt actgtttgaa aagaaaataa tcccataaac
    34141 ttatcaaata atggataaat atcccttaag aaaaatactc caagaattcc ccaaaataca
    34201 gaatattcaa cacttgttct tccatgtatg cttagagctt tactgctata ctcccatgat
    34261 acactactaa ataatttttc ttgaaaccaa gaacataaat attcaaatac ccctccaata
    34321 attgcagtta taaaaaatat tattattcca ttataattaa taagtctata aagtaaaact
    34381 gtaagcgcaa cccctcctat gccatataca ggactaattg gtccataaat taatcctttt
    34441 ctactttcaa aatgtccatt tcttatataa caccaaagca tctctacaca atatcctaaa
    34501 atagatccaa tagtaaatat ccatattatt ttataaatgt ttaactcata acttatattc
    34561 atgatttctc tgttgtcaaa aaactataaa tattttaata ataatgttca ttaaatatta
    34621 ttatattttt atagatttat aacaacactc acctccattc ttatttaatt ttcaagtaaa
    34681 ttttatttaa aaaaaaaaca ctttccttaa taaattaaag caagtgtttt taattcgttt
    34741 tattcatatc catttactat aacgttaagt tctccagttt gcggatcaat tacaagccca
    34801 tgtacattta cgtcactagg cattaatgga tgatttttta atgatgttac acttttttta
    34861 acagattctt ctacacatcc aaatccatga agccatttct ctacatctat accacagtgt
    34921 tttattgtta aaatagtctc ctctgatatt cctctagatt ccattttttt tatcatatct
    34981 ttagaattta agttgctcat accacaacca tgatgcccta caattaaaac atcttctgca
    35041 ttaaattcgt aaatagcaac aagtatactt ctaacaatac ttccaaaagg atgcattatt
    35101 gtagctccag cgttttttat tatttttgca tcaccatttt ttatattcat agcttttggt
    35161 aataactcag ttagtcttgt atccatacaa gataaaatca ctaatttttt ctctggtttt
    35221 ttagttgctt tatatttttc atactcctta ttcttaacaa aatttttgtt aaaatctaaa
    35281 atttcttcta atcttctcat aataaatacc tctaaatatt ttaaaattaa ttatttatac
    35341 caatatacca taaagcttta attatatctt taaaaatttt tatgcaaaaa agcgatttaa
    35401 actctttaat ttaaattcac ttaaaaattc tctctttatc actttttaac ttcatttaat
    35461 tatacaaatt gatatattaa taaattacac catatacttt tttaccatca caaggtatta
    35521 tatctgcata ttta
//
LOCUS       DAEVGI010000011        35331 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEVGI010000011 DAEVGI010000000
VERSION     DAEVGI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 35331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35331
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.011"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            359..1777
                     /gene="gadC"
                     /locus_tag="K8M77_000533"
     CDS             359..1777
                     /gene="gadC"
                     /locus_tag="K8M77_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBI7085059.1"
                     /translation="MPNKSSANVKTLTLFGFFAMTASMVMTVYEYPTFATSGFNLVFF
                     LIVGGLLWFLPVALCAAEMATVDGWEEGGIFAWVGNTLGERWGFAAIFFQWFQITVGF
                     VTMIYFILGALSYVLNWPALNSNPLIKFIGVLIIFWGLTFSQFGGTKNTAKIAKAGFV
                     FGVVVPAIILFILGIMYIAKGNPVHVDLSAHALIPDFTKVNTLVVFVSFILAYMGVEA
                     SASHVNKLENASKNYPLAMFILVVLAIVLNTVGGLTVAAVIPADQLNLSAGVVQTFHQ
                     LVVVNLGKSFDWITRIVALLLALGVMAEVSSWVVGPSEGMYAAAKKGLLPKKLTEVNK
                     HEVPVPLVLVQGLVVTIWAAVLTFGGGGNNVSFLTAISLTVVIYLVGYLLFFIGYIIL
                     ILKHGDLKRAYHVPGGKTFKMIVAIAGFVVSVFALVISFVPPSQLTGKSISEYLTILS
                     ISFIVTVLIPFIIYALHDKWNK"
     gene            1897..2952
                     /locus_tag="K8M77_000534"
     CDS             1897..2952
                     /locus_tag="K8M77_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HBI7085060.1"
                     /translation="MNYYISILFRAIPLLMGIICLTYGSYIYTLADKFGNEYIIASHV
                     IIFFTTICIALFTTAATIIRQLINKYNSFFKFILPTIGYLFAAISMVGGLYFALNNGG
                     NPAYFVSGNIGFGVGLIACCVSTVALSSTKFLLIPSNSKNLKEGEKPKGCFSKEMSYV
                     LIAIPIICTLIGLIFSFNLLRDTDKVPSLVAGFVLFGISLVCGSLISLVASVCRQIQN
                     TFTNKERWKWSILVIVLGSINLILGICVLLFSKNQTFIAPGYVLIGLGLVCYSISSKV
                     LLLASVWRRKSKLSKRIPLIPVITALACLFLSAYLSESELVNMAYFVPAHVMVGLGAI
                     CFTLFSIVSILESGTSN"
     gene            3538..4938
                     /locus_tag="K8M77_000535"
     CDS             3538..4938
                     /locus_tag="K8M77_000535"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI7085061.1"
                     /translation="MLFGKNELGSQFETPIFGTVESNEPIPKYKLAKKSIAPQVAYRL
                     IKDDLLDEGNARQNLATFCQTYMDDEAVKLMSETLEKNAIDKSEYPQTTDMENRCVNI
                     LADLWHAPKELNYMGTSTVGSSEACMLGGMAMKFRWRNRAKALGMDVTSKKPNLVISS
                     GYQVCWEKFCVYWDIEMRLVPMDEQHMSINVDKVLDYVDEYTIGVVGILGITYTGKYD
                     DIKALDKKLEEYNKTAKITVPIHIDAASGGMFAPFIEPELEWDFKLKNVVSISTSGHK
                     YGLVYPGIGWVLWKDKEYLPQELVFEVSYLGGKMPTMAINFSRSASQILGQYYNFLRY
                     GFEGYRQIHQRTKDVAMYLSSELEKTGLFEIYNNGENLPIVCYKLKDDVKVNWTLYDL
                     ADRLLMKGWQVPAYPLPEDLQNVIIQRFVCRADLSRNLADLLMRDLKAAIEDLNNARI
                     LSKTQVDNKGVQGFTH"
     gene            5144..5518
                     /locus_tag="K8M77_000536"
     CDS             5144..5518
                     /locus_tag="K8M77_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBI7085062.1"
                     /translation="MSDKDLNKCCGGNCGCGSEKEEKHEGCGCGGHGHDHGHDHHEGC
                     GCGDEHLEHFVVDLEDEEGNTITCDVIDAFEYKDEEYVLVQHPDESVYLFKSQGEEGE
                     LVLPSEEEFDEVVKFYEENVAE"
     gene            complement(5565..7214)
                     /locus_tag="K8M77_000537"
     CDS             complement(5565..7214)
                     /locus_tag="K8M77_000537"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBI7085063.1"
                     /translation="MKDVIYITGHKNPDSDSICAAIAYAEFKNKTQDTLAIPVRLGNV
                     SQETQYILDYFGVEAPQFLETVKLKVEDLEMDNIAPLAPEVSLKMAWNIMRDKNLKSI
                     PVADGNNHLLGMLSTSNITETYMDVWDSNILAKSSTSLDNILDTLSAEAQNINEERKV
                     FPGKVVVAAMQVESLKEFISEGDIAIAGDRAEIQAELIELKVSLLIVTGGHTPSKEII
                     ELAKKNNITVITTPHDSFTASRLIVQSLPVDYIMTKDNLVTVSTDDLVEDVKVIMSET
                     RYSNYPVIDENNKVVGSIARFHLISTHKKKVIQVDHNERGQSVHGLEDAEVLEIIDHH
                     RVADIQTSNPIYFRNEPLGSTSTIVAKRFFENGIRPSREAAGLLCGAIISDTLLFKSP
                     TCTPQDIKICRRLAEIAGIVPETFAKEMFRAGTSLKGKSIDEIFNSDFKPFTIGGIKI
                     GVAQVNTMDIEGFMPLKDEMLDYMNQKADSMGLEMIMLLLTDIINEGSQILVTGRNPE
                     IAEEAFKVKLEDSTTFLPGVLSRKKQVVPPLTQIITTRVSK"
     gene            7459..10008
                     /locus_tag="K8M77_000538"
     CDS             7459..10008
                     /locus_tag="K8M77_000538"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBI7085064.1"
                     /translation="MHEKELFRGLTTQEAKQRIEKFGLNEITEKKKVSAIKILLQQFN
                     DFIIWVLIGATIISGIMGDVADAITIFVIVVINGILGFVQEFKTEKSLDALKSLAAPT
                     CKVLRDGNIKVINANELTIGDVVILEAGDRVPADGEIFECTNFMIDESLLTGESVGVN
                     KTNNSKEASLIYMGTKVLKGKGRFRVTAIGMSTKMGSIADMLQDIEEEKSPLKERLDG
                     LGKILLVLCLAICALVTILGIIRGNDLTEMFLLGVSLAVAAIPEGLAAIVTVSLALGV
                     SRMLKRNALVKKLPAVETLGCTSVICSDKTGTLTQNKMTVKEIFVDGKHCRLDVDKIP
                     NSPILMKAFVYCNDCNYDFSKDKLDDVLNGDPTETALIKAYFNNVDLLKNTVSNVNRV
                     FDIPFDSSRKMMSVIVKENGREACYVKGAPERLINKCRYIYEEGRVKLLTSQKKQQIL
                     NVVENMSNRALRCIGGAYKVENLTRSESLENELIFLGIAGIIDPPRPEVKDSVIKCRL
                     AGITPVMITGDHKNTAFAIGKDLNIAKSQDQVITGEELDKLDDKELKKRVNKLRIFAR
                     VTPNHKLRIVKAFKQNGNIVAMTGDGVNDAPAIKEADIGVAMGISGTDVTKEASSMIL
                     MDDNFETIVSAVEEGRIIYDNIRKFIRYLLSCNLGEVLTMFLATIFYLPTPMLPIQIL
                     FVNLATDGLPAIALGVDPADKDIMNQQPRSKKEGIFARGLTEKILVRGCLIGICTLLT
                     FIVGGLYGMDLNTCRTMALSTLVMSQLLHVFECRSERHSIFEIKLFTNMYLVGAVGIS
                     ILMLLSIIYIPFFSGIFHTTVLGINHWLIVLFFSTIIFFINSLYLFIKVKK"
     gene            complement(10204..10611)
                     /locus_tag="K8M77_000539"
     CDS             complement(10204..10611)
                     /locus_tag="K8M77_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085065.1"
                     /translation="MGFKEKLSKYYTDSYLEKYGDRMAQFQGSVISEKIEEKTILWIF
                     HKLVVTLIVKPERSKQIIKCVYKKNRWFKKPTFISVKKGNNVIVQGLKSNKENEKEYI
                     ALNNILNLTTKQDLVPVDHSQLKKARQQQFKQR"
     gene            10869..11543
                     /gene="deoC"
                     /locus_tag="K8M77_000540"
     CDS             10869..11543
                     /gene="deoC"
                     /locus_tag="K8M77_000540"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBI7085066.1"
                     /translation="MDKQQLAKMIDHTILKPEADKASIEKLCKEALEYNFASVCINPT
                     NIELAAKLLKGSEVKVCTVIGFPLGANTMEVKAFETKDAIAKGADEVDMVINIGRLKD
                     KDYEYVEKDIKAVVDAADKKALTKVIIETCLLTEEEKVKACELAKKAGADFVKTSTGF
                     STGGATPEDIKLMRETVGPDMGVKASGGVRSIEDAEAVIKNGATRIGASASIAICEGK
                     VSDSTY"
     gene            11744..14029
                     /locus_tag="K8M77_000541"
     CDS             11744..14029
                     /locus_tag="K8M77_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7085067.1"
                     /translation="MRRELTPKEVIYNEDFTSIIGEKESYGNEYEGVFRKIDEALSIN
                     KEGFNVYLIDEFSKQKLKDLISHLKDKMKSRGKPKDICYVTLEDIRVPKVIFLENGMG
                     EKLEETLEYLKSFYYDEIYAFYNSSINKEKEEIINDIQKKRNYYIGDLIKSAKEEGFD
                     LKATSSGFAFIPLVDGEAMTEEEFDELEENSKGDISIKADKLKEGAESVLEELKNIEL
                     DSIEKLKELLRTYLENESASVKGKIKDNFKNENEAYNYLIDVCESLEKLLVDNYTINF
                     DDDEEKINEIFSKYVCNIIKNSKGQEAPKVIFEEDPSLNNLLGTIEYENHNGVYSTDV
                     KLIKSGSLLEANEGCIILRLSSLVNNANSYYYLRRTLLHGKINYDFNRGYLEVLSLNG
                     LNPDPIPIKVNVILIGDFESYDILYNNDEDFKKIFRVRAEFSSLIGIDENKRSLLDTI
                     DKIIIDNELIKISTSGINTIGKQLARKAGTRKKILWDIDEIERILLLGNEEAKNNNKS
                     FIDKDSIEEVINQCSEIEKDYLEMYEEKKIILDIEDRIIGSVNGLSVIDFGYMSFGKP
                     IRITCTCYKGSGKIMDAQRESNLSGNIHNKSLNILRGFLSSFFNSYEALPVDFQLSFE
                     QLYGKIEGDSASVAEVIAMISSLSKIPVDQSIAVTGSLNQFGQVQPIGGVNEKIEGFF
                     NVCKKIDTYIGKAVLIPESNKGELILNSEIEEAVRKGEFKIYLMKDINEALSTLLLND
                     TMSLEDIGNKIREEIKKFNDD"
     gene            14163..14498
                     /gene="spoIIAA"
                     /locus_tag="K8M77_000542"
     CDS             14163..14498
                     /gene="spoIIAA"
                     /locus_tag="K8M77_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBI7085068.1"
                     /translation="MWLDFEKIGNVLVVSLIGELDHHSAEEVRVKIDDRISRENIRKL
                     VLDFSGVTFMDSSGIGVIIGRYKKISMRSGEVNIISVSKSIKRVFELSGIFKIIKYYE
                     NLDEALKVG"
     gene            14512..14934
                     /gene="spoIIAB"
                     /locus_tag="K8M77_000543"
     CDS             14512..14934
                     /gene="spoIIAB"
                     /locus_tag="K8M77_000543"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBI7085069.1"
                     /translation="MDNKMRLEFLAKSENEGFARVSVSAFIAQLDPTIEELTDIKTAV
                     SEAVTNAIIHGYECDESKVVIIEASICEDEISITVEDNGIGIENLEEAREPLYTSKPE
                     LERSGMGFTVMETFMDSLEVYSEKNKGTKIIMKKKMNT"
     gene            14952..15707
                     /gene="sigF"
                     /locus_tag="K8M77_000544"
     CDS             14952..15707
                     /gene="sigF"
                     /locus_tag="K8M77_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBI7085070.1"
                     /translation="MDISSIEKDNFNYDKNTTLIVMAKEGNKEAMNKLIEVNTPLVSS
                     ICKRFTNRGYDYEDIFQIGSMGLVKAINNFNDSFNVKFSTYAVPMIIGEIKRFLRDDG
                     IIKVSRSTKTLAKKLHYNKEELIKKLNRDPTVDELAEYSGVDKEEVIVALESASSMQY
                     LYDTIHHDEGTPVLLIDKLSEKGEDDDMVNKIALREAISSLDSKGKQIIMLRYFKDKT
                     QIQVAKMLGISQVQVSRIEKKVLKIMKEKLEED"
     gene            15846..16313
                     /gene="spoVAC"
                     /locus_tag="K8M77_000545"
     CDS             15846..16313
                     /gene="spoVAC"
                     /locus_tag="K8M77_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBI7085071.1"
                     /translation="MYENDKKIRTKFDQLEQQIRPKPKLLRNCIMAFIVGGLICVIGQ
                     FVRNTFLYYGFTEEQVSSLTPMTMVFLGALLTGLGLYDKIAAKAGAGTIVPITGFSNA
                     MVSPAIEFKKEGFVMGVGAKMFTIAGPVLVYGTGTSVIVGLIYYLLLRFGIVG"
     gene            16319..17338
                     /gene="spoVAD"
                     /locus_tag="K8M77_000546"
     CDS             16319..17338
                     /gene="spoVAD"
                     /locus_tag="K8M77_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBI7085072.1"
                     /translation="MSNNTKRVGSQTIELKSKPRIIATYSVVGPREGQGPLKEYFDKI
                     LQDDCNGCESFEKAESSLMITAIEGVIKKGNVKEEDVDYLFSGDLLNQLTSTNFVARE
                     FKIPFFGVYGACSTMAESLSLSSMIMDGDFAKYAIASTSSHFSSAERQFRFPLEYGCQ
                     RKEYSQWTVTGSGAMLLGKSGDYPYVTHVTTGIVKDYGIKDADNMGAAMAPAAIDTIY
                     KHFIDTGRKPEDYDVIATGDLGAVGKKLTEELLKEYGYDISKVYIDCGEKIFDNEKQG
                     TFAGGSGCGCSAVVACGYLYRMLKEKKLKRVLLVSTGALLSSTSALQGESIPGIAHAV
                     AIEYI"
     gene            17407..17772
                     /gene="spoVAE"
                     /locus_tag="K8M77_000547"
     CDS             17407..17772
                     /gene="spoVAE"
                     /locus_tag="K8M77_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBI7085073.1"
                     /translation="MRILQDYINAFLVGGAICVIGQILIDKTKLTPAKVLVAFVSIGT
                     ILGALGIYEKLVQIGGAGATVPLPGFGYALAKATIKEVNEQGLMGAFTGGLKGAAGGI
                     TAAIFFGYLMALIFNPKTK"
     gene            17824..19479
                     /locus_tag="K8M77_000548"
     CDS             17824..19479
                     /locus_tag="K8M77_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBI7085074.1"
                     /translation="MKKFEKVIVNRPLGLICIFTILGIITYYFGSFNLIGTAFLVVPL
                     ILFSYLLLDIENFNIGIIFFLIAILSCTIYYEPKFQQEGAKEIRIIEVTDKVILGKID
                     GRIVEVRNQSGSELEYGEKIYGVCKFKRNINLDRGHVGQVFLKKVLKREKDLVYKIKN
                     IPKKYYENLSENFSFSESALLNAVLWGDKENLTYNQKDFLSDLGVIHLICISGFHIAL
                     LFSIIYKKFSFKIALSICTIYVILVGASSSALRALIMITLLKSSKRLFKTYDPLSSIS
                     LGAVILLIYKPYNLFSMGFLLSFGGTLGIILFYKKLLEGFYMLPPKLNEYLSLGLSAQ
                     AFIYPILVIAFGKFSINFLISTFLVTPIIVILLQILLISILIGGNLIGVIVFLINIIF
                     MVFRGILVFLDKVVWVTPYLSPVFAIAYMTMFLWIYMSLKGFKKFKIGVYLVIPVLLL
                     NLYVFGAKIKVVEDKWNKAVVIESGFKKVALVNNNSDYFKKSIMKKEYVNEVIHLKKE
                     SQFELTKRYSISIEDNFNTITLMPENSDYDIIDLIQKKSAYIL"
     gene            19523..20569
                     /gene="holA"
                     /locus_tag="K8M77_000549"
     CDS             19523..20569
                     /gene="holA"
                     /locus_tag="K8M77_000549"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBI7085075.1"
                     /translation="MIDLDGLYENLKKGKIEKSYILVGLDELLIKEGIENIIDNVLDE
                     GFKDLNLIRLDGNNLNFDDFMNACETLPFMSDKKVVVLNRTNIFRDKQDKETEKLYKQ
                     CVEYFQNTPEHCVLIAYTTLKDKRDRVNRLSKLKKLEKSSCIVSVEKLKGAKLQQKVG
                     RIFQRYNKNIGKIELKYFCDSVENNFDVIEREVDKIVNYVGEREITRKDIDLLLPPRY
                     EDDIFDLVELISIKRAKQAIDLMDELIGRGQDPIFILSQIREQFQKLHRVKIRLSEKY
                     RIDEIKKDFLEASRVNLPDFVIEKFINQSKKFSNKQLNKCINLCLYTEKKLKSNSNLN
                     KKTELELMIVSTVI"
     gene            complement(20665..20928)
                     /gene="rpsT"
                     /locus_tag="K8M77_000550"
     CDS             complement(20665..20928)
                     /gene="rpsT"
                     /locus_tag="K8M77_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBI7085076.1"
                     /translation="MANIKSAKKRIKVTEKKTLRNKMIKSALKTAIKKFEVAVEANNK
                     AEAATLYVEAARALDMSASKGVVHKNMAARKKSRLAAKLNAMA"
     gene            21124..22101
                     /locus_tag="K8M77_000551"
     CDS             21124..22101
                     /locus_tag="K8M77_000551"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBI7085077.1"
                     /translation="MYNVRTDLAVESREIYKHRYNREIDGVVFEEKTVEEDIKVTNVD
                     ILNEEGAKAMEKPIGRYVTIDIPDYTHYDGGIMDEVSHVVAASLEELINLPEERTALV
                     VGLGNWNVTPDAIGPKVVGKLMVTRHLKKVMPDIIDDSVRPVCAIAPGVLGITGIETG
                     EIIKSLVEKIKPDLVVCIDALASRKLERVARTIQISNTGISPGAGVGNHRMQINEESL
                     GIPVIALGVPTVVDAATIANDAMDLVLDEMINQADAGKEFYNILNNIDKNEKGMMIKS
                     LLDPYVGDLMVTPKEIDDIIESVSKIIANGINIALQPNMVLEDINKFLN"
     gene            22250..23335
                     /locus_tag="K8M77_000552"
     CDS             22250..23335
                     /locus_tag="K8M77_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBI7085078.1"
                     /translation="MRGINKREIPIGVIVLLSLLIIFMFRFMKIAASKDMRENLSYIQ
                     LLNAGMPVAKGTYYDENAYLESNITLKSLALETLNIKPLDPIELVMNEVPYFGAVNKI
                     ASIDKVNYVSAEKVSSFDLNKDSIDIVSEEESKKSAELEASKNSEVYDPSLKKELDQS
                     KPEVLIYHTHNSEGYSEERTSNNEEHNVVGVGTLVVKELEENYGISVIHDKTNHSASY
                     EQSYNKSRETVKKYTNEYDDFKMVIDIHRDSVGEHNKKNLTANINGESLAKVMFVTTR
                     NNQYFNNAESLAYRFINKANELFPDILRRQETYKYNRGQNAFNQDFNKNSMLIEVGAE
                     VNTSKEAQATAKYIARLIAEELNRKSE"
     gene            23390..23752
                     /locus_tag="K8M77_000553"
     CDS             23390..23752
                     /locus_tag="K8M77_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085079.1"
                     /translation="MKKFMTILTLGIIVSSIISWGLIGVNIENTEAFNNQNYELSEKV
                     DYDRIKEETGMDLTIFSEDNSPVKIYENKDEVYLVFKEHTLNLSKGLFGNILIGTFNL
                     VNKLINDINQFIGNYIIM"
     gene            23873..25675
                     /gene="lepA"
                     /locus_tag="K8M77_000554"
     CDS             23873..25675
                     /gene="lepA"
                     /locus_tag="K8M77_000554"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBI7085080.1"
                     /translation="MDKKKYIRNFSIVAHIDHGKSTLADRLLEKTGTLTQREMEQQVL
                     DTMELEKERGITIKSQAARLIYKRENGEEYILNLIDTPGHVDFTYEVSRSLAACEGAI
                     LVVDATQGIQAQTLANCYLALDNDLEIVPVINKVDLASARPDEIKQEIEDVIGIEAED
                     APLVSAKTGLNIEDVLEEIVEKIPAPEGDENAPLKALIFDSYYDSYKGVVCHIRVKDG
                     KVKPGTKIKLMSTDKVYEVVETGVFTPALMPLKEGLSAGEVGYITASIKNVRDARVGD
                     TVTEAARPTEEALPGYKPAIPMVYSGIYPVDGAKYEELKEALEKLQINDAALSFEPET
                     SVALGFGFRCGFLGLLHMEIIQERVEREFNLDIITTAPSVIYKVTKTNGESFDLTNPT
                     NLPPMTEIAYMEEPVVKASIITPTDYTGAVMELCQDRRGKFIDMQYLEETRVVIHYEI
                     PLNEIVYDFFDTLKSKTRGYASLDYELKGYEQSKLVKLDILLNGDNVDALSMIVPEVK
                     AYQRGRAIAEKLKEIIPRHMFEVPIQAAVGSKIIARETVKAMRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQLGTVEVPQEAFMSVLKVD"
     gene            26046..26315
                     /locus_tag="K8M77_000555"
     CDS             26046..26315
                     /locus_tag="K8M77_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085081.1"
                     /translation="MLDANKRDVKIKCRKFRNNGKATGVINRKNGDNISISVKISDLD
                     KFLAKHGDHSDLEINLEGEVINTSIIEVQRDLLVHNAININLREI"
     gene            26565..27698
                     /locus_tag="K8M77_000556"
     CDS             26565..27698
                     /locus_tag="K8M77_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBI7085082.1"
                     /translation="MDKISLYIHIPFCAQKCLYCDFPSFARKDHLRKAYIEALNKEII
                     SLREKHNNLEINTIFIGGGTPSVLESDELEYLLKEVAKLNMAKDIEYSMECNPGNLTE
                     EKLEVMKKYGVNRISMGLQAKQDNLLKGLGRIHNYKTFKENFLLAKKVGFNNINVDLM
                     FGLPNQKLNEWEETLREIISLEPAHISAYSLIIEEGTAFYNLYENNKLKLPTEEEERK
                     MYHLAKKILEENGFNQYEISNYAKKGKECRHNLAYWNMDNWIGVGSASASYMDGKRIK
                     NISSVEEYINSINEKGEAIEEIINNSKNDNMEEFMFMGLRKINGIDENEFKNRFSMNI
                     NDVYGEIINKYIDAGLLIRESGRIFLSEKGIEISNIIMADFLL"
     gene            27867..28913
                     /gene="hrcA"
                     /locus_tag="K8M77_000557"
     CDS             27867..28913
                     /gene="hrcA"
                     /locus_tag="K8M77_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBI7085083.1"
                     /translation="MSANKEVRLVIDDRKLQILRAIIQDYISTGEPVGSRTIAKKYNL
                     GVSSATIRNEMADLEDMGFLEQPHTSAGRIPSSRGYRLYVDRMIEFERLSSEEEGLIR
                     NSIIDGTLYEVDKIIKQTSALLSELTKMTCIVKAPSVHKSFVKSIQLLKVDDVSILCV
                     LVTDNGVIRNTVIKVKSVPISEELIKISKIITERLKNLTIEQINLEVISNLNRALNGY
                     EDIVNAVLPALYESLKGDETSEVFLEGTINIFNYPEYNNIHKAKEILELLHDKKSISE
                     LISDSDDMTVKIGDEIFVPEAKECSIISAGYHVGDRSLGTIALIGPRRINYSKVLSIM
                     TEVMKELNETLKNK"
     gene            28944..29570
                     /gene="grpE"
                     /locus_tag="K8M77_000558"
     CDS             28944..29570
                     /gene="grpE"
                     /locus_tag="K8M77_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBI7085084.1"
                     /translation="MVDNKDFNEELKESIQEELDNETKSENPNIDEEVEEVSEDIKAD
                     EKVIDFEELKALKEENTMFKSKTKKLENELEALKDRLLRISSEYENYRKRTDKEKERI
                     YTDACEDVLIKMLPVLDNLERALAVDGTVEDLKKGVEMTVRQFEDALEKLQVEEISTE
                     NGFDPELHQAMMVVEQEGSEPNQVAQVFQKGYKRGDKVIRHSMVTVTK"
     gene            29627..31486
                     /gene="dnaK"
                     /locus_tag="K8M77_000559"
     CDS             29627..31486
                     /gene="dnaK"
                     /locus_tag="K8M77_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBI7085085.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGGEPVVITNSEGARTTPSVVSFQA
                     NGERLVGQVAKRQAITNPEKTIMSIKRHMGTDYKVNIDGKDYTPQEISAMILQKLKAD
                     AEAYLGEKVTEAVITVPAYFNDAERQATKDAGRIAGLDVKRIINEPTAASLAYGLDKM
                     DSAHKILVYDLGGGTFDVSILDLGDGVFEVVSTNGDARLGGDDFDQRIIDYIAEDFKA
                     QNGIDLRQDKMALQRLKEAAEKAKIELSSSTQTLINLPFITADATGPKHIDMTLTRAK
                     FNELTHDLVERTIDIMKEALKSGNVSLNDIDKVILVGGSTRIPAVQEAVKNFTGKEPS
                     KGVNPDECVAMGAAIQAGVLTGDVKDVLLLDVTPLTLGIETLGGVATPLIERNTTIPA
                     RKSQIFSTAADNQTSVEIHVVQGERQMAADNKTLGRFTLSGIAPAPRGIPQIEVAFDI
                     DANGIVKVSATDKATGKEANITITASTNLSDAEIDKAVKEAEQFAEEDKKRKEAIEVK
                     NNAEQIVYQTEKTLNELGDKVSAEEKSEIEAKIEEVKKVKDGDDIEAIKKAMEDLTQA
                     FYKISEKLYQQNGGAQGEGFDPNNMGGANAGTGAANSNDDNVVDADFEVQDDK"
     gene            31600..32763
                     /gene="dnaJ"
                     /locus_tag="K8M77_000560"
     CDS             31600..32763
                     /gene="dnaJ"
                     /locus_tag="K8M77_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBI7085086.1"
                     /translation="MAKRDYYEVLGLQKGASDDEIKRAFRKMAMKYHPDRNPGDKEAE
                     ENFKEVNEAYDVLKDPDKKAKYDQFGHAAFDGSGGFGGGGFGGFDAGGFDFSEMGGFG
                     DIFESFFGGGFGGGSSRRRNAPQRGADLEYRLNITFEEAVFGCEKEISITRTENCETC
                     NGTGAKAGTSPKTCPKCNGSGQIRVQRQTPLGSFVSTTTCDQCGGTGKVIEDPCPDCK
                     GKGTVRKNRKITVKIPAGVDTGNIIPLRGQGEQGANNGPAGDLYIRVNVAPSKIFRRE
                     GSDIYYDYKISMAKAALGAEITVPTVDGNVKYKVPAGTQPGTKFRLKGKGVPYVNGGG
                     RGNQYVHMVVEVPKHLNKEQEEALKAFMKASGESVDDIDEKDEGFFSKFKKKK"
     gene            32939..33631
                     /locus_tag="K8M77_000561"
     CDS             32939..33631
                     /locus_tag="K8M77_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085087.1"
                     /translation="MIEVVGVKKKFRRKKVLENITFNVKKGEITALLGLNGVGKSTLL
                     KIIMGLVKQDEGEVLFNGEKLNYKTYENIAFVPDVLNTYGDMKIKDAFEYMSMMYEKW
                     DMDKAYEMLKNFKLTDDLKINKLSKGNIARVKLILGFARHPEYLLLDEPFSGIDIFTR
                     EKFIESLIGYMDNNIGILLTTHELKEVENIVDKVVFLSDGNIKIEFYVEDVRENEGLS
                     MVEKIREVHEGE"
     gene            33624..34445
                     /locus_tag="K8M77_000562"
     CDS             33624..34445
                     /locus_tag="K8M77_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085088.1"
                     /translation="MSKGNFKKLSYLLNLQILRDLKITIGSAAGIMLLNIAYFSYSLS
                     DELKIYLEKTENIGAIQMFPPIGSFSSFSNRGKGMLLTLLILVCFCLYCWYLWLGEFK
                     GENKSAYTLLTLPFPKKFIIIYKFLSACFYYFSLVFFQIVALLINYIIFNIMVPTDAI
                     VKESLITILSSSISSFYGLIPVSFVNLITNGLFFIAMILLTFLFAILERSFGVKGGIL
                     GVILGCSFLFLVLIFPGKMDFYMFERFIWFVFIAILYNLFGWFYSKYLLEKKVHV"
     gene            34461..35222
                     /locus_tag="K8M77_000563"
                     /pseudo
     CDS             34461..35222
                     /locus_tag="K8M77_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470563.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13662 a         3724 c         6439 g        11506 t
ORIGIN      
        1 aatattcttt tttatattta ttggataaat ttttaataaa gtgttaatta aataactctc
       61 ttgataagat taaattaaaa agaaagaaaa taaaagaaaa ttgaagtatt ttagagataa
      121 acaaaattaa tagaatgaat taataattaa tacggaaaaa tgaaagaatg aaacttttga
      181 ataaattgtt aacaaaggtt attatttaaa catagttagt agctattatt caagacaaaa
      241 atttagagta tgataaattt ttgtcttagg taatagttgt taattgttaa agcttaagta
      301 ttaaacttaa gattatcaaa ttataaatta aacaataaca acagaggagg cgtttattat
      361 gccaaataaa tcaagtgcaa atgtaaaaac acttactttg tttgggttct ttgccatgac
      421 agcatcaatg gtaatgactg tatatgaata ccctacattt gcaacatcag gatttaattt
      481 agtatttttc ttaatcgtag gcggactttt atggttctta ccagttgcat tatgtgcagc
      541 agaaatggca accgtagatg gatgggaaga aggaggtatc tttgcttggg taggtaatac
      601 tttaggtgaa agatggggat tcgcagcaat cttcttccaa tggttccaaa taacagttgg
      661 atttgttaca atgatttatt ttatattagg ggctctatct tatgtgctaa attggccagc
      721 attaaatagt aatccattga taaaatttat aggtgtttta attatatttt ggggattaac
      781 tttctcacaa tttggaggaa ctaaaaatac agccaaaata gctaaagcag gatttgtttt
      841 cggagttgta gttccggcaa ttatattatt tatattaggt ataatgtaca tagcaaaagg
      901 taatccagta catgttgatt taagcgcaca tgctcttata cctgatttta caaaagtaaa
      961 tacattagtt gtattcgtat catttatttt agcatatatg ggagttgaag catcagcatc
     1021 acatgttaat aaattagaaa atgcaagtaa aaactatcca ttagctatgt ttatacttgt
     1081 tgtattagca atagttctta atacagtagg tggtttaaca gtagcagcag ttataccagc
     1141 tgatcaatta aacctaagtg ctggtgttgt tcaaacattc catcaattag tagttgtaaa
     1201 cttaggtaaa agttttgatt ggataacaag aatagtagca ttattattag ctttaggagt
     1261 tatggcagaa gtaagttcat gggttgtagg tccttcagaa ggtatgtatg cagcggctaa
     1321 aaaaggatta ttacctaaaa aactaactga agttaataaa catgaagttc cagttccatt
     1381 agtattagtt caaggtttag tagttactat atgggcagct gttttaacat ttggcggtgg
     1441 tggaaataac gtttcattcc ttactgcaat atcattaaca gttgtaatat atttagttgg
     1501 atatttatta ttcttcatag gatatattat tttaatatta aaacatggag atttaaaacg
     1561 tgcttatcat gtaccaggtg gaaaaacatt taagatgata gtagcaatag caggatttgt
     1621 tgtatcagta tttgctttag ttatttcatt cgtaccacca tcacaattaa caggaaaaag
     1681 tatatcagaa tatttaacta tactatcaat aagtttcata gtaacagttt taattccatt
     1741 tataatttat gctttacatg ataaatggaa taaatagcag aaataaatta gagttatatt
     1801 tgaagtagtc taaaagtata ggctacttca aatattttat agagaaatat tttaaaattt
     1861 tgaaatttta actatataat aaaagaggtg aggagtatga attattatat aagtatatta
     1921 tttagagcaa taccattatt aatgggaata atatgtctta cttatggatc atacatatat
     1981 acacttgcag ataaatttgg taatgaatat ataatagcta gtcatgtaat aatatttttt
     2041 actaccatat gtatagcttt attcactact gcagcaacaa taataagaca attaataaat
     2101 aaatataatt cattttttaa gtttattctc cctaccattg gatatttatt tgcagcaata
     2161 agtatggtag gaggattata ttttgcttta aacaatggag gaaatcctgc atattttgta
     2221 tctggaaata taggttttgg tgtgggactg atagcatgtt gtgtatctac agtggcatta
     2281 tcatcaacaa agtttttgct tataccaagt aatagtaaaa atttaaaaga aggtgaaaaa
     2341 cctaaggggt gttttagtaa agaaatgtcc tatgtactta tagctattcc tataatatgt
     2401 actttaatag gattaatatt ttcatttaat ctcttaagag atacagataa ggtacctagc
     2461 ttggtagctg gatttgtact ttttggtata tctttagttt gtggaagttt aatatcatta
     2521 gtagctagtg tttgtagaca aatacaaaat acttttacta ataaggagcg ttggaaatgg
     2581 tccatattag ttattgtatt aggaagtata aaccttatat taggaatatg tgtgctttta
     2641 tttagtaaaa atcaaacttt tatagcaccg ggatatgtat taataggttt agggttagta
     2701 tgctatagta tttcaagtaa ggtattatta cttgcttcag tgtggagaag aaagagtaag
     2761 ttatctaaga gaattccttt aatacctgtt ataacagcct tagcttgttt atttttatca
     2821 gcatatttat cagaatctga attagttaac atggcatatt ttgtaccagc tcatgtcatg
     2881 gttggattag gagctatatg cttcacatta ttctccatag tgtcaatatt agagagtggt
     2941 acatcaaatt aaatttatgt attttatgga agtgaatttt taaggattca cttctttttt
     3001 tataaatatt taatgaaaat aaaggtttat aaatattgaa tctattaagg tgttattgaa
     3061 aagaaatttt ttacttaaat atttaataaa atgttaatga aatataaatt ttaaaatata
     3121 ttttatataa ggatatattt aatcaacatt ttgttatata tgcattattt tattaaaata
     3181 tgaaataata agtttgtttg aaataaaata attaaaaata tggatcttat tttaagtatt
     3241 tattaataaa aatcattcta aaatgtttta tagagaaaaa aagagtaaat agaaggaatt
     3301 aagagtattg gagagaaaat ttaaatgtta ataaataaat aataaaatat taacaaaatg
     3361 taacgaattg acgaatttaa atattatacc aaaaggtgta ttatataaat aacttgatga
     3421 ggtagtcatt aaggcaatat agataaaatg atttatataa ctaaaaatta ctcttaattt
     3481 cttgaaagtt atgaagagat taagagtggt ttttagctaa attaggaggt atttaaaatg
     3541 ctttttggaa aaaatgaatt aggtagtcaa tttgaaacac ctatatttgg aacagttgaa
     3601 agtaatgaac ctattccaaa atataaatta gcaaaaaaat caattgcacc acaagttgca
     3661 tatagattaa taaaagatga tttactagat gaaggtaatg cacgtcaaaa cttagcaaca
     3721 ttttgtcaaa catatatgga tgatgaagca gtaaaattaa tgtcagagac attagagaaa
     3781 aatgctatag ataaatcaga atatccacaa acaacagata tggaaaaccg ttgtgtaaat
     3841 atattagctg atttatggca tgctccaaaa gaattaaatt atatgggaac ttcaacagtt
     3901 ggatcatcag aagcttgtat gcttggtggt atggctatga aatttagatg gagaaataga
     3961 gcaaaagctt taggtatgga tgttacatct aaaaaaccaa acttagtaat ttcttcagga
     4021 taccaagtat gttgggaaaa attctgtgtt tattgggata tagaaatgag attagttcca
     4081 atggatgaac aacatatgag tataaatgta gataaagttt tagactatgt tgatgaatat
     4141 acaattggtg tagttggtat attaggtata acttatactg gtaaatatga cgatataaaa
     4201 gctttagata aaaaattaga agaatacaat aaaacagcaa aaataacagt tccaatacat
     4261 atagatgctg cttcaggtgg tatgtttgct ccatttatag aaccagaact agagtgggac
     4321 ttcaaactta aaaatgtcgt ttcaataagt acatcaggtc ataaatatgg attagtttat
     4381 ccaggtatag gttgggtatt atggaaagat aaagaatact tacctcaaga attagtattt
     4441 gaagtaagct atttaggtgg aaaaatgcct acaatggcaa taaacttctc aagatcagct
     4501 agccaaattc ttggtcaata ctataacttc ttaagatacg gatttgaagg ttatagacaa
     4561 atacatcaaa gaacaaaaga tgtagctatg tacttatcaa gtgaacttga aaaaactggt
     4621 ttatttgaaa tatataataa tggtgaaaac ttaccaatag tttgctacaa attaaaagat
     4681 gatgttaaag taaactggac attatatgat ttagcagata gattattaat gaaaggatgg
     4741 caagtacctg cttatccatt accagaagat ttacaaaatg taataattca aagatttgta
     4801 tgtcgtgcag atttaagtag aaacttagct gatttattaa tgagagattt aaaagcagct
     4861 atagaagatt taaataatgc aagaatttta agcaaaactc aagtagataa taagggagta
     4921 caaggattta ctcactagta taaaaaaact ctccttaaag caaaaacttt aaggagagtt
     4981 tttttattat aaattataat tactagaaaa aatattttct attgattata atattatatt
     5041 atgttaagaa tataattaaa gattataaag aaaatgcttt tattattttg tatttaaggt
     5101 tacaattaat ataaagtaaa ttttccacaa gggaggaaag attatgtcag ataaagattt
     5161 aaacaaatgc tgcggaggta actgtggttg cggaagcgaa aaagaagaaa agcatgaagg
     5221 atgcggatgt ggtggacacg gtcacgatca tggacatgac catcatgaag gatgcggatg
     5281 cggagatgaa catttagagc atttcgttgt agatttagaa gatgaagaag gaaacacaat
     5341 aacttgtgat gttattgatg cttttgaata taaagatgaa gagtacgttt tagtacaaca
     5401 tcctgatgaa tcagtttacc tattcaaatc tcaaggtgaa gaaggagaat tagttcttcc
     5461 ttcagaagaa gaatttgatg aggttgttaa attctacgaa gaaaatgtag ctgaataata
     5521 aaaaaagatg atgtcttaca tcatcttttt tattttaagt aaaattattt actaactctt
     5581 gttgtaatta tttgagttaa tggaggtaca acttgtttct ttcttgaaag gactccaggt
     5641 aagaaagtag ttgaatcttc taatttaact ttaaaagcct cttcagctat ttctggattt
     5701 cttcctgtaa ctagtatttg tgaaccttca tttattatgt cagtaagtaa taacataatc
     5761 atttctaatc ccattgaatc agccttttga ttcatataat ctaacatttc atcttttaaa
     5821 ggcataaatc cttctatatc catagtgtta acttgagcaa cacctatttt aatacctcca
     5881 attgtgaaag gtttaaaatc ggagttaaat atttcatcaa tagattttcc ttttaaggaa
     5941 gttccggctc taaacatttc tttagcaaat gtttcaggaa ctattccagc tatttcagca
     6001 agtcttctgc aaatttttat gtcttgtgga gtacaagtag gtgatttgaa taataatgta
     6061 tctgaaatta tagcaccaca taaaagtcca gcagcttctc ttgatggtct tattccattt
     6121 tcgaagaatc ttttagcaac tattgttgaa gtacttccaa gaggttcatt tctaaagtat
     6181 ataggattac tagtttgtat atctgcaact ctatgatggt cgattatttc taatacttca
     6241 gcatcttcta aaccatgtac tgattgacct ctttcgttgt gatcaacttg aataactttt
     6301 ttcttatgag tagaaattaa gtggaatctc gcaattgaac caactacttt attattctca
     6361 tctattacag gataattact gtatctagtt tcactcatta ttacttttac atcttcaact
     6421 aaatcatcag tagatacagt tactaagtta tctttagtca taatatagtc aactggtaaa
     6481 ctttgaacga ttaatcttga tgcagtaaat gaatcgtgag gtgttgtaat aacagttata
     6541 ttatttttct tagcaagttc tattatttct ttagaaggtg tatgtccacc tgttactatt
     6601 aatagagaaa cttttaactc tattaattca gcttgtattt cagctctatc tccagctata
     6661 gctatgtcgc cttcgcttat gaattctttt aaagattcaa cttgcatagc agcaactaca
     6721 acttttcctg ggaatacttt tctttcttca tttatgtttt gagcctcagc agacaatgta
     6781 tctaatatat tgtctaatga agtagaactt ttagcaagaa tattactatc ccaaacatcc
     6841 atgtatgttt ctgttatgtt tgatgttgat aacattccaa gtaaatggtt atttccatca
     6901 gcaacaggta ttgattttaa gtttttatct ctcattatgt tccaagccat ttttaatgaa
     6961 acttctggag ctaatggagc tatgttatcc atttctaagt cttcaacttt tagtttaact
     7021 gtttctaaaa attgaggtgc ttctacacca aaataatcaa gtatgtattg agtctcttgg
     7081 ctaacatttc ctaatctaac tggtatagca agagtatctt gagttttatt tttaaattca
     7141 gcatatgcta tagcagcaca tattgaatct gaatcaggat ttttatgtcc tgtaatgtat
     7201 ataacatcct tcatttaggt tgcctccttt gttattctaa tttttttaat tcatgaatat
     7261 attttaattc ctaaattatg aaaagtaaag tttaacaaca ctcattttat catagtttaa
     7321 tagaattttc tatgaggtaa aatttaagag ttaattttat catagtatgt tacataagaa
     7381 aaaatttagt tctaaaaacc agttatttaa acatatatta caatagattg ttaggaaaaa
     7441 aaggaggaac aaaaagttat gcatgagaaa gaactcttta gagggttaac aactcaagaa
     7501 gctaaacaac gaatagaaaa atttggatta aatgaaataa cagaaaagaa aaaggtatct
     7561 gctataaaaa tattattaca acaatttaat gattttataa tatgggtttt aattggagct
     7621 actattatat caggaataat gggagatgtt gcagatgcta ttactatatt tgttattgtt
     7681 gtaataaacg gaattttagg atttgtacaa gaatttaaga cagaaaaatc tttagatgct
     7741 ttaaaatctt tagcagcacc tacttgtaag gtattaagag atgggaatat taaggtaatt
     7801 aatgctaatg aattaactat aggagatgtt gtaatattag aggctggaga tagagtacct
     7861 gctgatggag aaatttttga gtgtacgaac tttatgattg atgaatcatt acttactgga
     7921 gaatcagttg gagttaacaa aacaaataat tctaaagaag cttctctaat ttacatgggg
     7981 actaaagtat taaaaggtaa aggaagattt agagtaacag ctataggtat gagtacaaaa
     8041 atgggatcta tagcagatat gttacaagac atagaagagg aaaaatctcc tcttaaagag
     8101 agattagatg ggttaggaaa aattttactt gtattatgtt tagctatatg tgctttagtt
     8161 actatattag gtattataag aggaaatgat ttaacagaaa tgttcttact aggtgttagt
     8221 ttggcggtag cggctatacc agaaggatta gcggctatag taacagtttc tttagcttta
     8281 ggggtttcta gaatgttaaa aagaaatgcc ttagtaaaga aattaccagc tgttgaaaca
     8341 ctaggatgta cctcagttat atgtagtgat aagactggta ctttaactca gaataagatg
     8401 actgttaaag aaatttttgt agatggaaag cattgtagac tagatgtgga taaaattcct
     8461 aattcaccta ttttaatgaa agcctttgtt tattgtaatg attgtaatta tgacttttca
     8521 aaggataagt tagatgatgt attaaatggt gatcctacag aaacagcctt aataaaggca
     8581 tattttaata atgtagatct attaaaaaat acagtatcta atgtaaatag agtttttgat
     8641 ataccttttg attcaagcag aaaaatgatg tcagttatag ttaaagagaa cggaagagag
     8701 gcttgctatg taaaaggtgc tccagaaaga cttataaata aatgtagata tatatatgaa
     8761 gaaggaagag ttaaattact aacttctcaa aagaaacaac aaatattaaa tgttgttgag
     8821 aatatgagta atagggcact aagatgtata ggaggagcat ataaagtaga aaatttaact
     8881 agaagtgaaa gtcttgaaaa tgaacttata ttccttggaa ttgcaggaat aatagatcca
     8941 cctagaccag aggttaagga ctctgttata aaatgtagat tagcaggaat aactcctgta
     9001 atgataactg gtgaccataa aaatactgcc ttcgctatag gaaaagattt aaatattgca
     9061 aaatctcaag atcaagtaat aactggagaa gaattagaca aattagatga taaagaactt
     9121 aaaaagagag ttaataagct gagaatattt gctagagtta ctcctaatca taagcttaga
     9181 atagttaagg cttttaagca aaatggtaat atagttgcca tgactggaga tggagtaaat
     9241 gatgctccag ctataaaaga agctgatatt ggcgttgcta tggggatttc tggtacagat
     9301 gttacaaagg aagcttcttc aatgatttta atggatgata actttgaaac tatagtatca
     9361 gcagtagaag agggaagaat aatatatgat aatataagaa aattcataag atatttattg
     9421 tcatgtaatt taggcgaggt tttaacaatg tttttagcta ccatattcta tcttccaact
     9481 cctatgttac ctatacaaat actttttgta aatttagcta cagatggact accagcaata
     9541 gccttaggag tagatcctgc tgataaagat ataatgaatc aacaacctag aagtaagaaa
     9601 gagggtatat ttgctagggg gcttactgag aaaattttag taagaggatg tttaattgga
     9661 atatgtacat tactaacctt tattgttggt ggattatacg gaatggattt aaatacatgt
     9721 agaactatgg ctttatcaac attagttatg tctcaattat tacatgtttt tgaatgtaga
     9781 tcagaaagac attcaatttt tgaaataaaa ttatttacta atatgtactt ggtaggtgca
     9841 gtagggatat caatacttat gttattaagt attatttaca ttcctttctt tagtggaata
     9901 ttccatacca cagtactagg tataaatcat tggttaatag ttttattctt ctcaacaata
     9961 atattcttta taaatagctt atacctattt atcaaagtaa agaaataaat ttaatgagat
    10021 atataaatta ttattttttt aatataaaaa tggtgtatat ccaaataatt ttttataaaa
    10081 cttacaaaaa tatgaagaga atatctagat aagctttaga atttaaatta ttagcaaaat
    10141 ctagtaatta tcagaatatg tttgtaagct ttaaataaaa aagctgtatt aatatacagc
    10201 tttttatctt tgtttaaatt gttgttgtct agctttcttt aattgtgaat ggtctacagg
    10261 cactaagtct tgtttagtag ttaagttaag tatgttattt agagcaatat actctttttc
    10321 attttcctta tttgatttaa gtccttgaac tataacatta tttccttttt tcacacttat
    10381 gaaagtaggt tttttaaacc aacgattctt cttatatacg cattttatta tttgcttact
    10441 tctctcaggt ttaactatta aagtaacaac taatttgtgg aatatccaaa gaatagtttt
    10501 ttcttcgatt ttttcagaaa ttacgcttcc ttgaaattga gccattctat cgccgtattt
    10561 ttcaagataa gaatcagtgt aatatttaga taatttttct ttgaaaccca taattataac
    10621 tccttttaag ttgacgaatt tacatttatt attactataa atacatatta tatgatagat
    10681 agtattataa cataatatta agtatattaa aatactttga tattgtccaa agtcccttag
    10741 aataagaatt ttatataaac atatattgct aaagtttatt taatgtaaat acttaaatta
    10801 tagacttatt agaatttata ttctataatt aaatatgtaa tgaaaatgtt atcttaggag
    10861 gaagtagtat ggataaacaa caattagcaa aaatgataga ccacactata ttaaaaccag
    10921 aagctgataa ggcatcaata gaaaagcttt gcaaagaagc tttagaatac aattttgctt
    10981 cagtttgcat aaaccctact aatatagaat tagcagctaa acttttaaaa ggcagcgaag
    11041 taaaggtttg tacagtaata ggtttccctt taggagcaaa cactatggaa gttaaagctt
    11101 ttgaaactaa agatgcaata gcaaaaggtg cagacgaagt tgatatggtt ataaacatag
    11161 gaagattaaa agataaagat tatgaatatg tagagaaaga tataaaagct gttgtagatg
    11221 cagctgataa gaaagcgctt acaaaagtta taatagaaac ttgtctttta actgaagaag
    11281 aaaaagttaa agcttgtgaa ttagctaaaa aagcaggagc agactttgtt aaaacatcaa
    11341 ctggattctc aacaggtgga gcaactccag aagatattaa attaatgaga gaaactgtag
    11401 gaccagatat gggagttaaa gcatcaggtg gagtaagatc aatagaagat gctgaagctg
    11461 taatcaaaaa tggtgctact agaataggag catcagcttc aatagctata tgtgaaggta
    11521 aagtttcaga ttcaacatat taatatataa ctaaattaaa taagatattt acttagtaaa
    11581 gcctatagaa aaatttctat aggctttttt gtcgaagaaa aaaataaata aaaaatagaa
    11641 aatgatggct aattttaaag ttatagaata ttaatataat aaagtactta taaagaggag
    11701 gttactctat taaagctaat attagtttta tgagtgaatt tttatgagaa gagagttaac
    11761 tccaaaagaa gtaatttata atgaagattt tactagtata ataggtgaga aggaatccta
    11821 tggtaatgaa tatgaaggag tttttaggaa aattgatgaa gcattaagta taaataaaga
    11881 aggatttaat gtttatctaa ttgatgaatt ttcaaaacaa aagcttaaag atttaatatc
    11941 acatttaaaa gataaaatga aaagtagagg caagcctaag gatatatgtt atgttacctt
    12001 agaagatatt agagttccta aggttatatt tttagaaaat ggaatgggag aaaagttaga
    12061 ggaaacttta gaatatctaa aatcttttta ttacgatgaa atatatgctt tttataattc
    12121 ttcaataaat aaggaaaagg aagagattat aaatgatata caaaaaaaga gaaattatta
    12181 tataggggat ttaataaaga gtgcaaagga agaagggttt gatttaaaag caacttcttc
    12241 aggctttgca tttattcctt tagtagatgg tgaagctatg acagaagagg aatttgatga
    12301 gctagaagag aatagtaaag gggatatttc tataaaagct gataagttaa aagaaggagc
    12361 ggaaagtgtt cttgaagaat taaaaaatat agagttagat tctattgaaa aattaaaaga
    12421 attgcttaga acctatttag aaaatgaatc agcaagtgtt aaaggaaaaa taaaggataa
    12481 tttcaaaaat gagaatgaag cttataatta tcttatagat gtttgtgaaa gtttagaaaa
    12541 gctattagtt gataattaca caataaattt tgatgatgat gaggaaaaga taaatgaaat
    12601 tttttcaaaa tatgtttgta atatcataaa aaacagcaag ggacaagagg ctcctaaggt
    12661 tatttttgaa gaagatccaa gcttaaataa tcttttagga actatagagt atgaaaatca
    12721 taatggagta tattcaactg atgtaaaact tataaaatca ggttcattac ttgaagcaaa
    12781 tgaagggtgc ataatactta ggttaagttc tttggtgaat aatgcaaata gctattatta
    12841 tttgagaaga actttacttc atggaaagat aaattatgat tttaacagag gatatttaga
    12901 agtactttca ttaaatgggt taaatccaga tccaatacct attaaggtaa atgtaatttt
    12961 aataggagat tttgaaagtt atgatatttt gtataataat gatgaagatt ttaagaaaat
    13021 atttagggtt agagctgaat tttcaagttt aataggaata gatgaaaata aaagatcttt
    13081 attagatact attgataaaa taataataga taatgagtta ataaagatct ctacttctgg
    13141 aattaatact attggaaaac aattagcaag aaaagctgga acaaggaaga agattctttg
    13201 ggacattgat gaaatagaga gaatattact tcttggaaat gaagaggcaa aaaataataa
    13261 taaatcattt atagataagg attcaataga agaagttata aatcaatgca gtgaaattga
    13321 aaaagattat ttagaaatgt atgaagaaaa gaagataatt ttagatatag aagatagaat
    13381 tattggaagt gtaaatggat tgtctgttat agattttggt tatatgagtt ttggaaagcc
    13441 tattagaata acttgtactt gttataaagg tagcggaaaa attatggatg cacaaagaga
    13501 aagcaattta agtggaaata ttcataataa atctttaaat attctaagag gctttttaag
    13561 tagctttttt aattcttatg aagctttacc tgtagatttt caactaagtt ttgagcagct
    13621 ttatggaaag atagaagggg atagtgcttc tgtggcagaa gtaattgcta tgatttcttc
    13681 tttaagtaaa atacctgtgg atcaaagtat tgcagtaaca ggttcattaa atcaatttgg
    13741 acaggtacaa ccaataggtg gagtaaatga aaaaatagaa ggatttttca atgtatgcaa
    13801 gaaaatagac acttatattg gaaaagctgt tttaatacca gaaagcaata aaggtgagct
    13861 tatattaaat agtgaaatag aagaggctgt gagaaagggt gaatttaaga tatatcttat
    13921 gaaagatata aatgaagctc ttagtacatt gcttctaaat gacactatgt ctcttgaaga
    13981 tataggaaat aaaattagag aagaaattaa aaaatttaat gatgattaat ataatgttaa
    14041 acctatagat tttgtttcta taggtttaat ttattttagt attatttgac ataaataatt
    14101 aaaatatgtt aaaatatgga aaagagacta ttagggaggt gcaactctat aagagtgatt
    14161 gtatgtggtt agattttgag aaaataggaa atgttttagt tgtaagttta attggagaat
    14221 tagatcatca tagtgcagaa gaagtaagag taaaaattga tgataggatt agtagagaaa
    14281 atataagaaa gcttgtattg gatttttcag gtgtaacttt tatggatagt tcaggaattg
    14341 gtgttattat tggaagatat aaaaagataa gtatgcgatc aggtgaagtt aacataatat
    14401 ctgtaagtaa aagcataaaa agagtttttg aattatctgg aatatttaag ataataaagt
    14461 attacgaaaa tttggatgaa gctctaaaag tgggttaatg gagggttaga tatggataat
    14521 aaaatgagat tagagttttt ggctaaatct gaaaatgaag gatttgcaag ggtgagtgta
    14581 tcagctttta ttgctcagtt agatccaact attgaagaat taactgatat aaagactgct
    14641 gtttctgagg cggtaacaaa tgctataatt catggatatg aatgtgatga gagcaaagtg
    14701 gttattattg aggctagtat ttgtgaagat gaaataagta taactgtaga agataatggt
    14761 ataggaatag aaaatttaga agaagcaaga gaacccttat atacatctaa accagaatta
    14821 gagagatcag gtatgggatt tactgttatg gaaacattca tggattcttt agaagtttat
    14881 agtgaaaaga ataagggtac aaaaataata atgaagaaaa aaatgaatac ataggcaggt
    14941 gaataaagtc tatggatata agtagtattg agaaagataa ttttaattat gacaaaaata
    15001 caactctcat agtcatggct aaagaaggaa ataaggaggc aatgaataag cttatagaag
    15061 ttaacactcc tttagtttct tcaatatgca agagatttac aaatagaggg tatgactatg
    15121 aggatatatt tcagattggg tcaatgggat tagttaaagc tataaataac tttaatgata
    15181 gttttaatgt taaattttca acttatgctg tgcctatgat tataggtgaa ataaagagat
    15241 tcttaaggga tgatggaata ataaaggtaa gtagaagcac taagacttta gctaaaaagc
    15301 ttcattacaa taaggaagaa ctaataaaaa aattaaatag ggatcctact gtagatgaat
    15361 tagctgaata ctctggtgtt gataaggaag aggtaatagt agccttagaa tcagcaagta
    15421 gtatgcaata tctatatgat actatacacc atgatgaagg aacgccggtt ctcttaatag
    15481 ataaacttag tgagaagggt gaagatgatg acatggtcaa taaaatagct ttaagagagg
    15541 ctattagttc attagattca aagggtaaac aaatcataat gcttaggtat ttcaaggata
    15601 aaactcaaat tcaagttgca aaaatgcttg gtataagtca agttcaagtt tctagaatag
    15661 agaaaaaggt actaaaaatt atgaaagaaa aactagaaga ggattgatta taaatgaaat
    15721 gttttaggag cattatttta tgtgaaagta atcatataaa ataatgctca tttttttgta
    15781 tagattaaaa ataaaaaaca aataatataa gtatatttta atagaaaagg gtgattaata
    15841 tagctatgta tgaaaatgat aaaaaaataa ggactaagtt tgatcaactt gaacaacaaa
    15901 taagaccaaa accaaagctt cttagaaatt gtataatggc atttatagta ggtggattaa
    15961 tttgtgtaat aggacaattt gtgagaaata cttttttata ctatggcttc acagaagaac
    16021 aagttagtag tttgacacct atgactatgg tttttttagg ggctctttta acaggacttg
    16081 gattatatga taaaatagcc gctaaggctg gagcaggtac tatagttcct attactggtt
    16141 tttcaaatgc catggtgtca cctgccatag aatttaaaaa agaaggattt gtaatgggag
    16201 taggtgctaa aatgtttact attgcaggtc ctgttttagt ttatggtaca ggaacctctg
    16261 taatagttgg actaatatat tacttattac ttagatttgg aattgtgggg tgataagaat
    16321 gagtaataat acaaaaagag taggatctca aacaatagaa ttaaaaagta aacctagaat
    16381 aatagctaca tatagtgtgg ttggtcctag agaaggtcag gggcctttaa aagaatattt
    16441 tgataaaata ttgcaagatg actgtaatgg atgtgaaagc tttgaaaagg ctgaaagtag
    16501 cttaatgata actgctatag agggagtaat taaaaagggt aatgtaaaag aagaggatgt
    16561 agattatctt tttagtggtg atttattaaa tcagttaaca tcaacaaact ttgttgctag
    16621 agaatttaaa attccttttt ttggtgtgta tggagcttgt tctactatgg ctgaatcatt
    16681 aagtttatca tcaatgatta tggatggtga ttttgcaaag tatgcaattg cttccacatc
    16741 atctcacttt agttcagcag aaagacaatt tagatttcct ttagaatatg gatgtcaaag
    16801 aaaagaatat tcacagtgga ctgtaactgg gtctggagct atgcttttag gaaagtcagg
    16861 agattatcct tatgttactc atgtaactac aggaattgtt aaggactatg ggattaagga
    16921 tgcagataat atgggagctg ctatggcacc agctgctata gatactatat ataagcattt
    16981 tattgataca ggaagaaaac ctgaagatta tgatgtaata gcaactggag acttaggggc
    17041 tgtaggaaag aaattaacag aggaattatt aaaagagtat ggatatgata ttagtaaggt
    17101 ttatatagat tgtggagaaa agatatttga taatgaaaaa caaggaactt ttgcaggtgg
    17161 aagtggatgt ggatgctcag ccgttgtagc ctgtggatat ttatatagaa tgttaaaaga
    17221 gaaaaagtta aaaagagttt tattagtttc cacaggagcc ttattaagtt ctacatctgc
    17281 cttacaagga gaaagcatac caggaatagc tcatgctgta gctatagaat atatttaaaa
    17341 atattattat aagtaagtta gctattttgt gtgagtattt ttttatagaa atttaataat
    17401 gaggagatga gaattttgca agattacata aatgcttttt tagtaggtgg tgcaatctgt
    17461 gtaataggac agatattaat agataagact aaactaacac cagctaaagt attagttgcc
    17521 tttgtttcaa taggaacaat attaggagct ttaggaatat atgaaaagct agttcaaata
    17581 ggtggagcag gagccacagt tccattacca ggctttgggt atgccttagc taaagcaact
    17641 ataaaagaag taaatgaaca aggattaatg ggagcattta ctggtggatt aaagggtgca
    17701 gcaggtggaa taactgcggc tattttcttt ggttacttaa tggcgttaat atttaatcca
    17761 aaaactaaat aaatgattat aatttctata aaaggtcaat aattttttcg aaggagagat
    17821 tctatgaaaa agtttgagaa agttattgta aatagacctt tagggttaat ttgtattttt
    17881 actattttag gaattataac atactatttt ggtagtttta acttaatagg tacagctttt
    17941 ttagttgtac ctttaatttt gtttagctat cttttattag atatagaaaa ttttaatata
    18001 ggtataatat ttttccttat agctatttta agttgcacca tctattatga gccaaaattt
    18061 caacaagaag gggctaaaga aataagaata atagaagtta cagataaggt catattagga
    18121 aagatagatg gaagaatagt tgaagtaaga aaccaaagtg gaagtgaact tgaatatgga
    18181 gaaaaaatat atggagtttg taaatttaag agaaatatta atttagatag aggtcatgtt
    18241 ggacaggttt tcttaaagaa agtattaaaa agagaaaagg atttagtcta taaaataaaa
    18301 aatataccaa agaagtatta tgaaaactta agtgaaaatt tttcattttc tgaaagtgca
    18361 cttttaaatg ctgttttatg gggagataaa gaaaatttaa cttataatca aaaggatttt
    18421 ttatcagact taggagtaat tcatttaata tgtatatcag ggtttcatat agccttactt
    18481 ttttcaataa tttataaaaa gttttctttt aagatagctt taagtatatg tactatttat
    18541 gtaattttag ttggagcatc tagttcagca ttaagagctt taattatgat aactttatta
    18601 aagtcttcta aaaggctatt taagacctat gacccattaa gttctatatc cttaggagca
    18661 gtaatactac ttatttataa accttataac ctttttagca tgggattttt attatccttt
    18721 ggtggaacct taggcataat tttattctat aaaaaattat tagagggatt ttatatgtta
    18781 ccaccaaagt taaatgaata cttaagctta ggactgtctg cacaagcttt tatatatcct
    18841 attttggtaa ttgcttttgg aaaattttct attaactttt taatatcaac ctttttagta
    18901 actccaataa tagtaatatt attacagata ctattaattt ctattttaat tggtggaaat
    18961 ttaattggag ttatagtatt tttaataaat ataatattca tggtatttag ggggatttta
    19021 gtatttttag ataaggtggt atgggttacc ccttatttat ccccagtatt tgcaattgct
    19081 tatatgacta tgtttttatg gatttacatg agtcttaaag gatttaaaaa gtttaaaata
    19141 ggagtttatt tagtcatacc tgttttgctt ttaaatttat atgtatttgg agctaaaatt
    19201 aaagttgtgg aagataaatg gaataaggct gtagtcatag aatctggatt taagaaagtt
    19261 gccttagtaa ataataatag tgattatttt aaaaaatcca taatgaaaaa ggaatatgtt
    19321 aatgaggtta ttcatttgaa aaaagagtct caatttgaac tgacaaaaag atattcaata
    19381 tccatagaag ataattttaa taccataact ttaatgccag agaatagtga ttatgatata
    19441 attgatttga ttcaaaagaa aagtgcgtat atactttaac ttatgtaaat aagtagctgg
    19501 gatttgaaag gttgatattt taatgataga tttagacggt ttatatgaaa atttgaaaaa
    19561 aggaaagata gaaaaaagtt atatattagt tggattagat gagcttttaa ttaaagaagg
    19621 tatagaaaat ataattgata atgtattaga tgaaggattt aaggatttaa acctaattag
    19681 attagatggg aataacctta attttgatga tttcatgaat gcttgtgaaa cattgccttt
    19741 tatgagtgat aaaaaagtag tagttttaaa tagaactaat atatttaggg ataaacaaga
    19801 taaagaaact gaaaaattat ataagcaatg tgtagaatat tttcaaaata caccagaaca
    19861 ttgtgtttta atagcatata caactttaaa ggataaaagg gatagagtta atagattaag
    19921 caaactaaaa aagcttgaga agagcagttg tatagtttct gttgaaaagt taaaaggggc
    19981 aaaactgcaa caaaaggtag gaagaatctt tcaaagatat aataaaaaca taggaaaaat
    20041 agaattaaag tacttttgtg actcagtaga aaataacttt gatgttatag aaagagaagt
    20101 tgataaaata gtaaactatg taggggagag agaaataact agaaaagaca tagatttact
    20161 acttcctcct aggtatgagg acgacatatt tgatttagtt gagcttattt caattaaaag
    20221 agcaaagcaa gccatagact taatggatga attaataggc agaggacaag atcctatatt
    20281 tatactaagt caaatcagag agcaatttca aaagttacac agggttaaaa taagactttc
    20341 tgaaaagtat agaattgatg aaataaagaa agatttttta gaagctagca gggtaaattt
    20401 acctgacttt gtaatagaaa aatttattaa tcagagtaag aagttttcta ataaacagct
    20461 taataaatgt attaatctat gtctatatac agaaaagaaa cttaagagta attcaaactt
    20521 aaataaaaaa acagagttag aacttatgat tgtaagtact gtaatataaa aaatccagat
    20581 tagataattc taatctggat tttttactta aagaaatact tctaataaaa gaaaatggtg
    20641 ttagaaattc taacacccat ataattaagc catagcgttt aacttagcag ctaatcttga
    20701 tttctttcta gcagccatgt ttttatgaac tactccttta gaagcagaca tatctaaagc
    20761 tctagcagct tcaacgtata aagttgcagc ttctgctttg ttattagctt caacagctac
    20821 ttcaaacttc tttatagcag ttttaagagc tgacttaatc atcttatttc ttaaagtttt
    20881 cttttcagta acttttattc ttttctttgc tgatttaata tttgccattc tagttcaccc
    20941 ccttcaactt ttatagcgta tctgcagttt tggggaatct gcgtacgagt tcattattaa
    21001 caaaggtaat tataacatca taaatatata agttcaagtc ttttttcgtg tttatcgaca
    21061 taattatcat ttttaaggag attatatata atatataaat ttaaattaaa aagaggtgga
    21121 aaaatgtaca atgtaagaac tgatttagct gttgaaagta gagagattta taaacataga
    21181 tataataggg aaattgatgg ggtagttttt gaagaaaaga ctgtggaaga ggatattaaa
    21241 gttacaaatg ttgacatatt aaatgaggaa ggcgctaaag ctatggaaaa acctataggc
    21301 agatatgtta ccatagatat ccctgattac actcattatg acggtggcat aatggatgag
    21361 gtttctcatg tggtggcagc ctccttagaa gaacttatta atttaccaga ggagagaacg
    21421 gctttagtag ttgggcttgg aaactggaat gttacacctg acgccatagg tcctaaggtt
    21481 gtgggaaaat taatggtaac aagacattta aagaaggtta tgccagacat aatagatgat
    21541 tcagtaagac cagtttgcgc catagcacct ggagttttag gcataacagg aattgaaact
    21601 ggagagataa taaaatcatt agtggaaaaa ataaaaccag atttagtagt ttgtattgat
    21661 gccttagctt caagaaaact agaaagagtg gcaagaacta ttcaaataag caatactgga
    21721 atatcaccag gggctggagt tggaaatcat agaatgcaaa taaatgaaga aagcttaggt
    21781 atccctgtta ttgccttagg agtaccaact gttgtggatg cagctactat tgcaaatgat
    21841 gccatggatt tagttttaga tgaaatgata aatcaagcag atgctggcaa agaattttat
    21901 aatatattaa ataacataga caagaatgaa aagggaatga tgataaagag ccttttagat
    21961 ccttatgtag gagatttaat ggttacccca aaagaaatag acgatataat agaatcagtt
    22021 tcaaaaataa tagcaaatgg aattaatata gcccttcaac ctaatatggt gttagaggat
    22081 ataaataaat ttttaaacta agactgggtt taaaattaaa agttttatta agatattttt
    22141 tagaattatg agtgaatttg aagaagaata aattaaatat ttacctaaag agagtaacat
    22201 aatatttaac ctatcctcat ataaatgaaa atatgaggga ggggataatg tgagaggtat
    22261 taataaaaga gagataccca taggagtgat tgttctttta tctttactta taatatttat
    22321 gtttagattt atgaagattg cagcaagtaa agacatgaga gaaaatttat catatataca
    22381 acttttaaat gcaggaatgc ctgttgcaaa gggaacatat tacgatgaga atgcttattt
    22441 agaaagtaat attacattaa aaagtttagc cttagaaact ttaaatataa agcctctaga
    22501 tccgatagag ttagtaatga atgaagtgcc ttactttggt gcagtaaata aaatagcttc
    22561 aattgataaa gttaattatg tttctgctga aaaagtatct tcatttgatt taaacaaaga
    22621 tagtatagat atagtttctg aggaagaatc taagaaaagt gcagaattag aagctagtaa
    22681 aaatagtgaa gtttatgatc cgagtttaaa aaaggaacta gatcagtcta agccagaggt
    22741 gcttatttat catactcata attcagaagg gtatagtgaa gagagaactt caaataatga
    22801 agaacataac gtagttggag taggtacttt agttgtaaaa gaacttgaag aaaactatgg
    22861 catatcagtt attcatgata aaacaaatca ctcagcttca tatgagcaat cttacaataa
    22921 atctagagag acagttaaaa aatatactaa cgaatatgat gattttaaga tggtaataga
    22981 tattcataga gattctgttg gagagcataa caaaaagaat ttaactgcta atataaatgg
    23041 agaaagttta gctaaggtta tgtttgttac aactaggaat aaccaatatt ttaataatgc
    23101 tgaatccttg gcctatagat ttattaataa agcaaatgag ctttttcctg atattttaag
    23161 aagacaggaa acctataagt ataatagggg acaaaatgca tttaaccaag atttcaataa
    23221 gaattcaatg cttattgaag ttggtgcaga agtaaatact tctaaagagg ctcaagctac
    23281 agcaaagtat atagctagat taatagcaga agaattaaac agaaaaagtg aataaaataa
    23341 aagaattttg aacatcctta tagatagtaa tctatggggg tgttttattt tgaaaaaatt
    23401 tatgactatt ctaactcttg gcataatagt ttcttctata atttcttggg ggcttatagg
    23461 tgttaatata gaaaatacag aggcatttaa taatcaaaat tatgaattaa gcgaaaaagt
    23521 tgattatgat agaataaaag aagaaacagg tatggattta actatattta gtgaagacaa
    23581 tagtcctgta aaaatatatg aaaataaaga cgaggtttat ttggttttta aagaacacac
    23641 tttaaactta agtaaaggct tatttggtaa catactaatt ggcaccttta acctagtaaa
    23701 taaattaatc aatgatatta atcaatttat aggaaactat ataataatgt aaagtaaagt
    23761 atattgacag tttaatggca cattgtgata taatgtaact tgacttattc tacaatgtgg
    23821 gggtgaaaac tgttcaaaaa ctttttaaat agtttagaga gcaggtttag atatggataa
    23881 aaagaagtat ataagaaact tttcaatcgt agctcatata gatcatggta aatcaacatt
    23941 agcagataga cttttagaga aaactggaac attaactcaa agggaaatgg aacagcaagt
    24001 attagatact atggagcttg aaaaagaaag aggaataaca ataaagtctc aagcagcaag
    24061 acttatctat aagagagaga acggcgaaga atatatatta aatcttatag atactccagg
    24121 tcacgtagac tttacatatg aggtttcaag aagtttagca gcctgtgaag gtgctattct
    24181 tgtagtagac gctactcaag ggatacaagc tcaaacttta gcaaactgtt acttagcatt
    24241 agacaatgat ttagaaatag tacctgttat aaataaggtt gacttagcta gtgctagacc
    24301 agatgaaata aagcaagaaa tagaagatgt tattggaata gaagcagagg atgcaccttt
    24361 agtatcagct aaaactggat taaacataga agatgtttta gaagagatag ttgaaaaaat
    24421 tccagcacca gagggtgatg aaaatgctcc acttaaggct ttaatatttg actcatacta
    24481 tgatagttac aaaggagttg tgtgtcatat aagagttaaa gatggtaaag ttaagccagg
    24541 aacaaaaatt aaactaatgt caacagacaa ggtttatgaa gtagttgaaa ctggagtatt
    24601 tacaccagct ttaatgcctt taaaagaagg attaagtgca ggggaagttg gatacataac
    24661 agcttcaata aagaatgtta gagatgccag agtaggtgat actgtaacag aggcagctag
    24721 accaacagag gaggcattac caggatataa accagctata ccaatggttt actcaggtat
    24781 atacccagtt gatggagcta agtatgaaga attaaaagag gcattagaaa agcttcaaat
    24841 aaatgatgct gcattatcat ttgaaccgga aacatcagta gctttaggat ttggatttag
    24901 atgtggattc ttaggattat tacacatgga aataattcaa gagagagttg aaagagaatt
    24961 taacttagat ataattacaa ctgcaccatc agttatatac aaagtaacta agacaaatgg
    25021 agaatctttt gatttaacaa acccaacaaa ccttcctcca atgacagaga ttgcatacat
    25081 ggaagagcct gttgttaagg cttcaattat aactccaact gattacactg gagctgtaat
    25141 ggaattatgc caagatagaa gaggtaaatt catagatatg caatacttag aggaaacaag
    25201 agttgttata cattatgaaa ttccattaaa tgaaatagta tatgacttct tcgatactct
    25261 taaatcaaaa acaagaggat atgcttcatt agattacgaa ttaaaaggat atgagcaatc
    25321 aaaacttgtt aagttagata ttttattaaa tggagacaac gtagatgctt tatctatgat
    25381 agttccagaa gttaaggctt atcaaagagg tagagctata gctgaaaaac ttaaagaaat
    25441 aattccaaga cacatgtttg aagttccaat tcaagcagct gtaggttcaa aaattatagc
    25501 tagagaaact gttaaagcta tgagaaaaga cgtattagct aagtgttatg gtggagatat
    25561 ttcaagaaag aaaaaacttc ttgaaaaaca aaaagaaggt aagaagagaa tgagacaatt
    25621 aggaacagtt gaagttcctc aagaagcatt catgtcagtt ttaaaagttg actaattaat
    25681 ttctttatat agaggcaatg atgaatagta ttaagtattc atcattgcct tttatcttta
    25741 aaatattaat aacagtaatt aaatttttga ttattcttaa aaatttttgt gaattctttt
    25801 ttcggtatac acaaacattt taatagctct taataaaaga tatttataag tatggggatt
    25861 tatttaattt taaaaattta atatatataa tttgcaaagt agttagtctt ttattactag
    25921 attattttta gatacattta atttaaattt ctaaaataaa tattttttat ttagtcatta
    25981 aaaggaaatc tcttcaatat atttatagta aattaataaa aattatgtag aggagaggta
    26041 ttattatgtt agatgctaat aaaagagatg ttaaaattaa gtgcagaaaa tttagaaata
    26101 atggtaaggc aactggtgtt ataaatagaa aaaatggaga taatatttct atcagtgtta
    26161 aaatttctga cttagataag tttttagcta agcatggaga tcatagtgat ttagagataa
    26221 atttagaagg tgaagtaata aacacttcaa ttatagaagt tcaaagagat ttattagttc
    26281 acaatgctat aaatataaat ttaagagaga tataaagcta aaaatttttt gagtaaactt
    26341 atttatatgg caatattaat cttaaatctt aaaattttag ttttatttaa atgtagatat
    26401 ttaattttgt gaaagaatca tataaaagtc gtgggtttat tcactcatga ctttttttat
    26461 tgtaatatga aagaaagact cataactaat aatttgtatt taactacaaa atttatttca
    26521 tattagttaa aaagtttatt tattttacat ggaggtaagt aactatggac aaaatttctt
    26581 tatatataca cattcctttt tgtgctcaaa agtgccttta ttgtgatttt ccttcctttg
    26641 ctaggaaaga tcatttaaga aaggcttaca tagaagcttt aaataaggaa ataataagtt
    26701 taagagagaa acataataat ctagaaataa acactatatt tattggagga ggaacaccaa
    26761 gtgttttaga gtctgatgag ttagaatatt tattaaaaga agtggctaaa cttaatatgg
    26821 cgaaagatat agagtactct atggagtgta atccaggaaa cttaacagaa gaaaagctag
    26881 aagtcatgaa aaagtatggt gtaaatagaa taagcatggg acttcaagct aagcaggata
    26941 atcttcttaa aggactagga agaatacata attataaaac ctttaaagaa aattttttat
    27001 tagcaaaaaa ggttggcttt aataacataa atgttgattt aatgtttgga ttaccaaatc
    27061 aaaaattaaa tgaatgggaa gagactttaa gagagataat atctttagag ccagcacata
    27121 tatcagcata tagtctaatt atagaagaag gaactgcttt ttataattta tatgaaaata
    27181 ataagttaaa gctaccaaca gaagaagaag agagaaaaat gtatcaccta gcaaagaaaa
    27241 ttttagaaga aaatggattt aatcaatatg agatttctaa ttatgctaaa aaaggaaaag
    27301 aatgtagaca taatttagca tattggaata tggataattg gataggagta gggtcagcat
    27361 cagcttctta tatggatgga aaaagaatta aaaacataag tagtgttgaa gaatatataa
    27421 attctatcaa tgaaaaggga gaagccatag aagaaataat aaataattca aaaaatgata
    27481 atatggaaga atttatgttt atgggactta gaaaaattaa tggaatagat gaaaatgaat
    27541 ttaaaaatag atttagcatg aatataaatg atgtttatgg ggaaataata aataagtata
    27601 tagatgcagg acttttaata agggagtcag gaagaatatt tctaagtgaa aaaggtatag
    27661 aaatatcaaa tataataatg gctgattttc tattataaaa tttaaatagg ttatagtata
    27721 aattgaagaa aataaaagaa aaaaagagat attaaagaat aaataaggta aaattcaaaa
    27781 atttcatttg aaatgataaa aaatggaatt gacaaaaaac ttggtaggta atatattaat
    27841 agtgtactag ttagcactcg ttaataatga gtgctaataa agaggtgaga ttagtgatag
    27901 atgatagaaa gcttcaaata ttaagagcta taattcaaga ctatatatca actggagagc
    27961 ctgttggttc aagaactatc gctaaaaaat ataatcttgg agtaagttca gctactataa
    28021 gaaatgaaat ggctgactta gaggatatgg gatttttaga acagcctcat acttcggcag
    28081 gaagaattcc atcaagcaga ggatatagat tatatgttga taggatgatt gaattcgaaa
    28141 gattatcctc tgaagaggaa ggtcttatta ggaattcaat aatagatggt accttatacg
    28201 aagttgataa aataatcaaa caaactagtg ctcttctatc ggaacttact aaaatgactt
    28261 gtatagtaaa ggcaccttca gtacataaga gctttgtaaa atcaattcag cttttaaaag
    28321 ttgatgatgt aagtatttta tgtgttttag taactgataa tggtgtaata agaaatacgg
    28381 tgataaaagt aaagagtgtt cctatatctg aagaacttat taaaattagt aagataataa
    28441 ctgaaaggct taaaaacctt actattgaac aaataaactt agaggttatt agtaatctca
    28501 atagagcttt aaatggatat gaagatatag taaatgcagt tttacctgct ctttatgaga
    28561 gcttaaaagg tgatgaaact tctgaggtat tcttagaagg aacaattaat atattcaatt
    28621 atcctgaata taataatatt cataaggcaa aagagatttt agagctttta catgataaaa
    28681 aaagtatttc agaacttata agtgatagtg atgatatgac tgtaaaaata ggtgatgaaa
    28741 tatttgtacc agaagcaaag gaatgttcca taatatcagc gggatatcat gtaggagata
    28801 gatctttagg tacaatagct ttaataggtc ctagaagaat aaattattct aaagtcttat
    28861 caatcatgac tgaagttatg aaagagttaa atgaaacttt aaagaataag taagtaaaaa
    28921 aagagaagag gtgtggctgc tttatggtgg ataataaaga ttttaacgaa gagttaaagg
    28981 aaagtatcca agaagaatta gataacgaaa caaaatctga aaatcctaat attgatgaag
    29041 aggtagaaga agtttcagaa gatattaagg ctgatgaaaa agttattgat tttgaagaat
    29101 taaaggcttt aaaggaagaa aatactatgt ttaaaagtaa aacaaagaaa ttagaaaatg
    29161 aattagaggc cttaaaagat agattattaa gaatatcttc agagtatgaa aattacagaa
    29221 aaagaacaga taaagaaaaa gaaagaatat atactgatgc atgtgaagat gttttaatca
    29281 aaatgcttcc agtattagat aacttagaaa gagccttagc tgtagatgga actgttgaag
    29341 atcttaaaaa aggtgttgaa atgacagtga gacaatttga agatgcttta gaaaaacttc
    29401 aagttgaaga aatatctaca gaaaatggtt ttgatccaga attacatcaa gcaatgatgg
    29461 ttgtggaaca agaaggctca gagccaaatc aagtagctca agtattccaa aaaggataca
    29521 agagagggga taaggttatc agacattcaa tggtaactgt aactaaataa tttaattagc
    29581 atttaacaga tttaaattat agatattgtt agaggaggaa ttaacaatga gtaaaataat
    29641 cggtatagat ttaggaacaa caaattcatg cgtagctgtt atggaaggtg gagaaccagt
    29701 agttatcact aactcagaag gtgctagaac aactccatca gtagtttcat tccaagcaaa
    29761 cggagaaaga ttagtaggtc aagttgctaa aagacaagca ataacaaatc ctgaaaaaac
    29821 aataatgtca atcaaaagac atatgggaac tgactataaa gttaatatag atggaaaaga
    29881 ttatacacca caagaaatat cagcaatgat acttcaaaaa ttaaaagcag atgcagaagc
    29941 ttacttagga gaaaaagtaa cagaagctgt tatcacagtt ccagcttact tcaatgatgc
    30001 tgaaagacaa gcaactaagg atgctggtag aatcgctggt ttagatgtta agagaataat
    30061 aaacgaacca acagctgcat cattagctta tggattagat aaaatggata gtgctcacaa
    30121 aatcttagta tatgacctag gtggtggtac tttcgatgta tctatcttag acttaggaga
    30181 tggagtattt gaagttgtat caacaaacgg agatgctaga ttaggtggag atgacttcga
    30241 ccaaagaatt atagattata tagcagaaga ttttaaagct caaaatggaa ttgatttaag
    30301 acaagataaa atggctcttc aaagattaaa agaagctgct gaaaaagcta aaattgagtt
    30361 atcatcatca actcaaacat taatcaactt accatttata actgctgatg caactggtcc
    30421 aaaacacata gatatgacat taacaagagc taaattcaat gaattaactc atgacttagt
    30481 tgaaagaaca atagacataa tgaaagaagc cttaaaatca ggtaatgttt cattaaatga
    30541 tatagataaa gtaatcttag ttggtggatc aacaagaata ccagcagttc aagaagctgt
    30601 taaaaacttt actggaaaag aaccttcaaa aggagttaac ccagatgagt gcgtagcaat
    30661 gggtgctgct atccaagctg gtgtattaac tggtgatgtt aaagatgtat tattattaga
    30721 tgttactcca ttaacattag gaatcgaaac tttaggagga gttgcaactc cattaatcga
    30781 aagaaataca actatccctg caagaaagag ccaaatattc tcaactgcag cagataacca
    30841 aacttcagtt gaaattcacg tagtacaagg tgaaagacaa atggcagctg ataacaaaac
    30901 tttaggtaga tttactctat caggaattgc tccagctcca agaggaatcc ctcaaataga
    30961 agttgctttc gatatagatg ctaacggtat agttaaagtt tcagcaactg ataaagctac
    31021 tggaaaagaa gctaacatta caatcacagc ttcaactaac ttaagcgatg ctgaaataga
    31081 taaggctgta aaagaagcag aacaatttgc tgaagaagat aagaagagaa aagaagctat
    31141 agaagttaaa aataatgctg agcaaattgt ttaccaaaca gaaaaaactt taaatgaact
    31201 tggcgataaa gtttcagctg aagaaaaatc agaaatagaa gctaaaatcg aagaagttaa
    31261 aaaagttaaa gatggtgacg atatagaagc tatcaagaaa gctatggaag atttaactca
    31321 agcattctac aaaatatcag aaaaattata ccaacaaaat ggtggagcac aaggtgaagg
    31381 gttcgatcca aacaacatgg gtggagctaa tgctggaaca ggtgctgcaa atagcaacga
    31441 tgacaatgtt gtagatgctg atttcgaagt tcaagatgat aaataataat taaaactatc
    31501 acaagactta aagctttgat ataatataaa aagaattaat agagggaagg ctcaatagtc
    31561 ttcccttttt ataagtaaac ttataggtgg tgaataacag tggctaaaag agattactat
    31621 gaagtgcttg gacttcaaaa gggtgcttca gatgatgaga taaaaagagc ttttagaaaa
    31681 atggctatga aatatcaccc ggatagaaat cctggagata aggaagcgga agaaaacttt
    31741 aaagaagtaa atgaagcgta tgatgtttta aaagaccctg ataaaaaagc taaatatgat
    31801 caatttggtc atgctgcttt cgatggaagc ggtggctttg gtggtggcgg atttggtggc
    31861 tttgatgctg gtggattcga cttctcagaa atgggtggct ttggagatat ttttgaatca
    31921 ttctttggtg gaggatttgg tggcggatca tctagaagaa gaaatgctcc acaaagaggt
    31981 gctgatttag aatatagatt aaatataaca tttgaagaag ctgtatttgg atgtgaaaaa
    32041 gaaatatcta taactagaac tgaaaattgt gaaacttgta atggtacagg tgctaaagca
    32101 ggtacatctc ctaagacttg tccaaaatgt aatggttcag gtcaaataag agttcaaaga
    32161 caaactcctt taggaagctt tgtatctaca acaacttgtg atcaatgtgg tggaacaggt
    32221 aaagtgattg aagatccatg tccagattgt aagggaaaag gaactgttag aaagaataga
    32281 aagataacag ttaagatacc agcaggtgta gatactggaa acataatacc tttaagagga
    32341 caaggtgagc aaggtgctaa taatggtcct gcaggagatt tatatataag agtaaatgtt
    32401 gctccatcta agatatttag aagagaaggt tcagatattt actatgatta caagataagt
    32461 atggctaagg ctgcattagg agctgaaatc actgttccaa ctgtagacgg aaatgttaaa
    32521 tataaagttc cagcaggtac tcaaccagga actaagttta gattaaaagg aaaaggtgtt
    32581 ccttacgtta atggtggagg aagaggaaac caatacgttc atatggtagt agaggttcca
    32641 aagcatctaa ataaagagca agaagaagca ttaaaagcct ttatgaaggc atctggggaa
    32701 tcagttgatg atatagatga aaaagatgaa ggattcttct caaaatttaa aaagaaaaaa
    32761 tagtaaattt aaaatactct agttagaaat gactagggta ttttttgttt atataaattt
    32821 aaaaaagtaa ataatttaca agtatatgta ttagtgatat aatacactta agattggtgt
    32881 attaatgaag taatacatga gtacaaaaat atattaacat aaatctaggg gtgattaaat
    32941 gatagaagtg gttggtgtta aaaagaaatt tagaagaaaa aaagttttag agaatattac
    33001 ttttaatgta aagaagggag aaataactgc tttactagga ttaaatggag tgggaaagtc
    33061 tactctttta aagataatta tgggtcttgt taagcaagat gagggggaag tactttttaa
    33121 tggagaaaag ctaaattata agacctatga aaatattgca tttgtacctg atgttttaaa
    33181 tacttatggt gacatgaaaa taaaagatgc ctttgaatat atgagtatga tgtatgaaaa
    33241 gtgggatatg gataaagctt atgaaatgtt aaaaaatttt aaattaactg atgacttaaa
    33301 aataaataaa ctttctaagg ggaatatagc aagggtaaaa cttatactag gttttgctag
    33361 acacccagaa tatttacttt tagatgaacc tttttcaggt atagatattt ttacaagaga
    33421 gaagtttata gaatctttaa ttggttatat ggataataat attggaattt tactaacaac
    33481 ccatgaatta aaagaggttg aaaatatagt tgataaagtt gtctttttaa gtgatggaaa
    33541 tattaaaata gaattttatg ttgaagatgt aagagaaaat gaagggttgt caatggtaga
    33601 gaaaataagg gaggtacatg aaggtgagta agggaaattt taaaaagtta agttatcttt
    33661 taaatttaca gatattaaga gatttaaaaa taacaatagg atcagctgct ggaattatgc
    33721 ttttaaacat tgcctatttt agttatagtt taagtgatga attaaagatt tatttagaaa
    33781 aaacagagaa tataggtgct attcaaatgt ttcctcctat aggttctttt agtagttttt
    33841 ctaatcgtgg aaagggtatg ctattaacgc ttttaatact agtatgcttt tgtttatatt
    33901 gttggtatct atggcttggt gagtttaaag gggaaaataa aagtgcatat actcttttaa
    33961 cattgccatt tcctaaaaag tttattatta tatacaagtt tttatcagcg tgtttttatt
    34021 atttttcttt agttttcttt caaatagtag cattgcttat aaattatata atatttaata
    34081 ttatggttcc aactgatgct atagtaaaag aaagtttaat tactatatta tctagttcaa
    34141 taagtagttt ttatggattg attccagtta gttttgttaa tttaataact aatggcttgt
    34201 tttttatagc aatgatatta ttaacttttt tatttgcaat attagagaga agttttggag
    34261 taaagggtgg aatattaggg gtaatattag gatgttcttt tcttttctta gttttaatct
    34321 tcccaggaaa gatggatttt tatatgttcg aaagatttat ttggtttgtt ttcatagcaa
    34381 tcttatataa tttgttcgga tggttttatt ctaagtattt acttgaaaaa aaggttcatg
    34441 tgtaaggagg attagaaatt atgaaaaaaa gagtaccttt ggtgttaaca ataatatttt
    34501 taagtgcatt tataattttt tctggaatat ttatttacat taattgttcg ccaataaagt
    34561 ttaaagacat atatggaagt agaagtgaac ttggagatgt tgacttagtt ttttataaag
    34621 atagagatat ttttgaagaa gaagtaactg ttgaagctga aacagttagt agaagaaatg
    34681 ttattaatga gaggcgtttt gatgatattg atgtgcttaa agataagaag ttttttagag
    34741 gaatttatcc aacaagaaat actttctttg aggatgacga tgtaatggtt gatgttacta
    34801 atatatacgg taatggtatt caaaagcttg aagtcagatt taaggataaa aaaactaaca
    34861 cttatgaaac ttttaaggtt aaagtagatg aatatattag aaataataat atagataaag
    34921 tcacttataa agatggaaaa ataaatatat tattttcaat gaattacgag gaaaataata
    34981 tagtatttgg ggaaataaat ttatcagata agaagtttaa tacagttgat ataataaatt
    35041 tagatgaaga attgcactta aatgaagaat tttctcatat aaattcaata cctcaagagt
    35101 ttggaacttt attaaatgct gaagaagata cagtatatta taaattaaac gaacttgata
    35161 agaccgataa gaggggaata tatagtgatg agagcattat tgaattaaat gtaaattcta
    35221 aattgcaata tcaaattgaa ctagtcctta attatgattt aagaactaat taatataatg
    35281 aatatatttt atctaagtgt atttcaaata actcttctgg agtcttgtaa t
//
LOCUS       DAEVGI010000012        35182 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEVGI010000012 DAEVGI010000000
VERSION     DAEVGI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 35182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35182
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.012"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            331..597
                     /locus_tag="K8M77_000564"
     CDS             331..597
                     /locus_tag="K8M77_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085089.1"
                     /translation="MKGSSGPVRAKKPKRSTAYTLSLYLGWSSIFAALYFFIVGLPFS
                     IPGIIAGYIAFKKHNEGKLPLILNIIGFLLTVFTWVLFMKIPLK"
     gene            704..794
                     /locus_tag="K8M77_000565"
     tRNA            704..794
                     /locus_tag="K8M77_000565"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:739..741,aa:Ser,seq:tga)
     gene            800..890
                     /locus_tag="K8M77_000566"
     tRNA            800..890
                     /locus_tag="K8M77_000566"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:834..836,aa:Ser,seq:gct)
     gene            complement(928..1110)
                     /locus_tag="K8M77_000567"
     CDS             complement(928..1110)
                     /locus_tag="K8M77_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085090.1"
                     /translation="MDIFFYFIFFTFILYILLKEFFKKVFKEAFIELLNEDNEKLKLF
                     IKECLREHDFSKRNKN"
     gene            1359..2240
                     /locus_tag="K8M77_000568"
     CDS             1359..2240
                     /locus_tag="K8M77_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBI7085091.1"
                     /translation="MKVEKLNDIKNNSKEIIFNSNSKIVLFSDCHRGDGTYKDSLAPN
                     INIYLSAIRYYYKNKFTYIEVGDGDELWKFKNIQEIYDMNYEIYEVLEKFKEKKRLYM
                     IYGNHDKDKSKIKFLRKNKRRNRFNHSASDFYSTLNIYESLVLVHEESKKDFFVIHGH
                     QIDFLNNELAFLSKFLVRYVWAILEAFMGFKDPTSPAKSNNKRNLFDEKISKWAEENK
                     TRVILGHTHKTLFPKSRNESTYFNIGCCVLPRTITAIEIERGEISLIKWTIKADEKGS
                     LFVGRDIIGGPIRIENY"
     gene            complement(2330..2494)
                     /locus_tag="K8M77_000569"
     CDS             complement(2330..2494)
                     /locus_tag="K8M77_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085092.1"
                     /translation="MKIGDTFKKFMDTTAEIETKLGNPMACFLEEQEREEKEKKAHHK
                     EVKAKGKNDN"
     gene            2703..6179
                     /gene="addB"
                     /locus_tag="K8M77_000570"
     CDS             2703..6179
                     /gene="addB"
                     /locus_tag="K8M77_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBI7085093.1"
                     /translation="MGLKIIYGRAGTGKSTFCINQIKKKINNSPTNKLILLVPEQFTF
                     QTENKVLNAIGERYVLNAEVLSFKRLAHNVFNECGGATRTIMGDAGKSMLIFKVLEDL
                     GDNMTVFKNASRQKGFIDIASKTITEFKKYNVNNEVLDLTINEIEDENLKMKMEELKD
                     VFNEFNSRLHEGYVDEEDQLLLLNEKLDGCSLYDGAEIWIDEFSSFTPNQLSVIGKLL
                     KKAKSVNITLSIDEVNSPKGESDLFVATKNTEKRLMNLIQEEGIAFNGYINLNEDIPY
                     RFKENRELAHIERQLYAYPFKQYRGENNSLRLYRANNNYDEIEFVAKDILRLVREKQY
                     RFKDISVICRDVDNYEKVISAIFAEYEIPYYIDKKIDIASNPLIVFINSAVDIISKNW
                     TYESMFKYLKTGLIKEFRGIEGAELIDELENYVLAYGIKGKKWMEEWVNYSSSILKEE
                     EISEENKQRLERLNDIRETIVTPLDEFNKECKGKKTLKEFAIILYEFLDSKLDIMDTI
                     DKYVEYFKENDMAIEAKEYSEVRDIFIDVLEQAVDVLGNEVMNLDEFMKVLNIGLSQY
                     EMGLIPVALDQVNIGDITRIKSRGTKALYIIGVNDGVLPSASKEEGILSDNDREILLE
                     KGISLASDTRTKIFEEQFLVYTAFTIAEEYLVVTYPLADFEGKSQRPSIIVHRLKKIL
                     PNVKEESEGFKLVNDKYDKISAKIPTLNELMIAIRKNYDGAEIEDYWKYVYDWYLREP
                     KWKERIEYVRKGLEYTNLENNISKEKAKKLYEDNKNKISLSVSRLERYAQCPFAYYIQ
                     YGLKAKDRKIYEFTAPDLGSFMHEILDEFTNEIKEKDLKWSDLSKENCRNIINSLVDN
                     QVKNNKSSILNSSKRYSYFTDRFKRILTKSVMVISEQMKRSDFEIYKNELAFGFSKDV
                     NSIKLDLPSGESFYLNGRIDRIDKLNLDGETYLRIIDYKTGSKKFDLNKFYNGLQMQL
                     LVYLDALINNSENIVENQAMPGAILYFRIDDPILKSKGDLTEEEIKSEILKELKLEGL
                     LLDDVKVVKAMDNTLEPGTHSLIIPANMKKAGDLGKNKALITMEQFELLRKYVNEKMV
                     EICQNMIEGKIDIEPCKENKNIVCDYCNYSHICQFDSSLEDNRYKVIPKKKDEDIWKS
                     INEKVGGEVNGD"
     gene            6169..9984
                     /gene="addA"
                     /locus_tag="K8M77_000571"
     CDS             6169..9984
                     /gene="addA"
                     /locus_tag="K8M77_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBI7085094.1"
                     /translation="MGTKWTEEQELAINTRKCNLLVAAAAGSGKTAVLVERIIKMITE
                     GENPVDIDKLLVVTFTNAAASEMRERIGDAISKALEKDPSSEVLQRQLALLNRASITT
                     MHSFCLEVIKNNFHLIDLDPGFRIGDQTECELIKQDILADLFEDMYAKDDECFKDLVE
                     AYGGSKSDDNLNSIILKFYNFIMSGPWPESWLKDKVEEFNINSIEELEGKKWIEVLKE
                     SIILDLNNAYSMLTQARDIAEMGGGLEPYLVNIYPEIIQVEELRIALSEGIVKFYNKL
                     MGASFGRLKSVRKASVDDEKALEKTKSLRDESKKIIENLRDNVFETSLEEAVLGMKKM
                     YPLMKCLSGLIIEFSNRYRDKKREKDILDFNDLEHLCLEILIDKDEEGNIKPSQVALE
                     FKDKFEEVLVDEYQDSNTIQETIVGMVSRRDVENPNVFMVGDVKQSIYKFRQANPELF
                     LEKYINYREFEDSNRKIMLYKNFRSREEIINGVNYIFKTLMSNTVGELEYDEKEALNL
                     GASYGELNEENVEKEYIDEIENLKVAGDIELNILNKAGNKDYSDEDELGEEEEDLDSI
                     QLEARIIGKKINELMNPEDGSHYMVFDKDLGKYRKIKYKDIVILLRATKNWADTFVDE
                     LGTYGIPVYADTGTGYFQTIEIRTILALLHIIDNPMQDIYILSSLRSPIFSFTSEEFA
                     DLRLLNKDKYFFEIIKEVVDGIYDESISKDLKGKCKYFLDYLNKWREKAAYMPIDEFI
                     WFLYSDTSYYGYVGTMPNGVQRQANLRILFQRAKQYESTSFKGLFNFINFINKLKKSS
                     GDMGSAKILGENENVVRIMSIHKSKGLEFPVVILGGTGKQFNKMDLREDILLHETLGI
                     GTNCIDVKKRIKYDTLQKHAIKKKCELEVLSEEMRILYVAFTRAKEKLIITGAVSDLE
                     KSCENWCKASASSEDNRINPGNVLKGKSYLDWIGMALTKHKDGDAIRNVGNGDITLNL
                     DDKSNWSFKSFDRSELLETNNNKKEKNNIDIFESNNWIESKKDIKEVIEIRNRLGFKY
                     KYIESCNTPSNISVTELKRAHQEEEFMQESYNIIDNESSEENKKEKIKRKPRFMEERQ
                     EEFSAAKKGTITHFVMQHIDLDKVTYIDEIREEVLKMVKKELLTEEEGKVVNVFKIQK
                     FFKSELGQRMLSSYKSGKKVYRELPFITEIPSSIIEKNLDPKIYGEEKVRLQGIIDAF
                     FEEEDGYVLLDYKTDYVKEGEEEAFINKYKIQINLYKDTLNKILGEEVKEAYLYSFYL
                     EKELKISKE"
     gene            complement(10023..11291)
                     /locus_tag="K8M77_000572"
     CDS             complement(10023..11291)
                     /locus_tag="K8M77_000572"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBI7085095.1"
                     /translation="MKLPNNVQYILEKFDSNGFEAFIVGGCVRDSLLNKKPQDYDITT
                     NAFPEKIEELFDKTIPTGIKHGTVTVLIDKTPYEVTTYRVDGEYLNNRKPKDVKFVSN
                     IEEDLSRRDFTINAMAYSPYLGFKDCFNGKEDLKNKLIRCVGDPDKRFSEDALRMLRA
                     IRFSCQLNFKIEKLTAESIRKNFKLIKNISMERIQSEFTKIILSNEPDRGLMLLRKLG
                     FSDFLVKEFKNLKLINCYDLYDDIHDTYGLINSLPKKLHIRLAGLFYKVFNSENAVEK
                     CRTILKKLKYDNNTINDTCNLVENINSISCNMTRKKLKLLINSVGTENIFDLLDLQKS
                     YLSYMDEYDTECIDILKNRVSDILASKEPIFIKDLAITGNDLITELNFKPGKNIGVIL
                     NFLLENVMQTPELNNKEDLLNLSKQFYSYN"
     gene            11407..12399
                     /locus_tag="K8M77_000573"
     CDS             11407..12399
                     /locus_tag="K8M77_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBI7085096.1"
                     /translation="MERKDRLMEIPVKKIGEFVKGERVEGFFLLKSVTLKTTNSGGKK
                     YLDLVLSDKTGDILGKVWEIKPEHEELKSNTVFKIRGTVNSWQGTLQLKVEHASKVTE
                     EDNINLDDFVQSAPYTSDEMFNTIKDTVDNMNDIDIKNILNRVLEINKDKLMYYPAAK
                     SNHHSIKGGLLYHITTMLKLAENICVIYDFLNRDLVYAGVILHDMAKIKEMSSNEMGI
                     VDEYTLEGTLLGHITQGIKEIEVIGKEVNADSKIIMLLQHMVLSHHYEPEYGSPVKPL
                     IPEAEMLHYLDVMDARMYDMKKAIKETKENEFSERIWSLDSRRIYNHGMYEKNN"
     gene            complement(12430..13590)
                     /locus_tag="K8M77_000574"
     CDS             complement(12430..13590)
                     /locus_tag="K8M77_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HBI7085097.1"
                     /translation="MKNKKLLISISLSIFLLLIIVVSYFIFSYSNNLNKFKEDLSSNN
                     YEDAAYCFNNGTKSIILKKTFIKSSEDIITKKLSEIENEYYSGDISEEETVKDLNGLL
                     SLNTSNEEIFNFKNTIPLIKDSKDSFDIGISKLDEENYLEALNYFKSVHNLSDCKAQA
                     LECEETCLDKIREPILETVDEYISNEKYSKGIEYLDSKINSLPDDPKLQEKRDELENL
                     RLEHLEAYSQENIDKATARTAPVMEYYKKLNEDTINQFDITSNTNYLVFVNIAEQKTY
                     VYEGSKNDWTLDKTFTCSTGIEGKETPVGIFTVQTRAPWFFSPKYGQGGKYYVQFMGN
                     YLFHSIPFNSDKTTVSDPTLGVPASHGCIRLSVEDSKWLYDNVQNGSKIIIY"
     gene            13782..15002
                     /gene="pepT"
                     /locus_tag="K8M77_000575"
     CDS             13782..15002
                     /gene="pepT"
                     /locus_tag="K8M77_000575"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBI7085098.1"
                     /translation="MKKVHERFLEYVKVDTKSDETTRVTPSTKGQLELGKILAEELKK
                     IGVDEVRISDKGYVYACLKSNCDKDIPKIGFISHMDTAPDMSGKNVNPKIVENYDGKD
                     IELGNGYTLSPSFSPELPMYKGQTLITTDGTTLLGADDKAGVAEIITAIEYLINHPEI
                     KHGDIKIGFTPDEEIGEGADHFDVEGFGADFAYTLDGGRIGELEYENFNAASAKVEII
                     GKNVHPGSAKGKMINSILVAHEFVSMLPLNEVPEKTEGYEGFSFLLDIEGEVEKTSLS
                     FIIRDFDKEGFKNRKERFNEIANELNKKYGEGTVTVTLKDQYMNMKEMIEPRMHIVET
                     AEKAMKQCGIEPIKKPIRGGTDGARLSFMGLPTPNIFTGGENFHGRYEYISVNSMEKA
                     VEVILNIIKIYAEK"
     gene            complement(15108..15983)
                     /locus_tag="K8M77_000576"
     CDS             complement(15108..15983)
                     /locus_tag="K8M77_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBI7085099.1"
                     /translation="MKKFVLFTDSAADLPKSMVKDLDINYLGLICNIDNTEYIDNIDS
                     ELTPKAFYNKLREGIMPSTSQINSFRFVEAFEEYVKQGISILYLAFSSALSGTYNSSL
                     IAREELLEKYPDADIKIVDTRAACLGQGLLVYYAAQMKKDGKSIDEIYSWVEENKNKL
                     CHYFTVDSLDHLKRGGRISSTAAAIGSLLSIKPMLYVNDAGELHNFAKAKGRKKSLKM
                     LFQELEKRIVNPNEQTIFIAHSDCIEDAETLAEMIREKYPVKDILIDYIGIVIGSHTG
                     IGTLAVFFLGDTKEP"
     gene            16173..16249
                     /locus_tag="K8M77_000577"
     tRNA            16173..16249
                     /locus_tag="K8M77_000577"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16207..16209,aa:Arg,seq:acg)
     gene            16421..17560
                     /locus_tag="K8M77_000578"
     CDS             16421..17560
                     /locus_tag="K8M77_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family protein"
                     /protein_id="HBI7085100.1"
                     /translation="MNYDFIITILDEHNESSILLAEKLKPKEIIYLYKKDEELKVLNS
                     LRQFYLNKFPNCNFSDEKLGKDTISSIEEIIKRMKSKNTAICLNQGNKKDILIMYTLA
                     LKHNIDGFFLDIPKEELLKLNLESVQCEKCNFVDLDVEDIIDSIGASIVVDSTEISEI
                     NIIENMTNYIASNLDLWKKYKIRLSDNSVFIHDESNPRSIKIDKELLSREEVLLLDKI
                     LNFLEKNGQIKVKELEQCLKVTFQNEFIKGFIFKSGTWLEVLTKNIIEEIKSIDDIKS
                     GVLFLWNDKESRVKNELDVVAIKDSVLICVSCKDSKKYDEVALNELNVYSEQLGGENV
                     IKILVATHPPIKSSISKRAKEMGINLVVFDGNKKAFKEELEKIIK"
     gene            complement(17750..18634)
                     /locus_tag="K8M77_000579"
     CDS             complement(17750..18634)
                     /locus_tag="K8M77_000579"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBI7085101.1"
                     /translation="MIKIYNRKTKAYDVEQVAGLKYINWSYASPIGKSFLELFIKKKM
                     FSKLYGNFCDSPLSKKKIKAFIDEFNIDMSLCNKNIDEFENFNDFFARTLTPEARPID
                     YSENILISPGDGRLSAFENIDLDKVVQIKGYTYSLKELIDDPKVAEEFEGGTCLILRL
                     CPTDYHRFHFVDSGTCSESKKISGFYYSVNPIALNNVSELFCKNKREWSIFTSDNFGE
                     ILHVEVGATCVGTILQTYSPEKRVKKGEEKGYFKFGGSTTILFFKKDTIKIDSDIVEQ
                     TKLGFETKVNMGETIGHK"
     gene            18797..19366
                     /locus_tag="K8M77_000580"
     CDS             18797..19366
                     /locus_tag="K8M77_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI7085102.1"
                     /translation="MNRTKKAIFEAAINVFATSGYNGSTVDEIASKANVAKGTLYYNF
                     KSKEEIFNFVISKGLEIWHEKLTDIENLEDEPIEKLKKLFKMQFELLYENRAFFKMVM
                     SQLWGKETRQDELRNKITEYIEGIERILKEAISKKQIRECDISLLAHSLFGSLISTSL
                     YELSRDKEFNVNKVIDEITINILDGIVIK"
     gene            19421..19495
                     /locus_tag="K8M77_000581"
     tRNA            19421..19495
                     /locus_tag="K8M77_000581"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19454..19456,aa:Arg,seq:cct)
     gene            19640..20905
                     /locus_tag="K8M77_000582"
     CDS             19640..20905
                     /locus_tag="K8M77_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI7085103.1"
                     /translation="MDPYTFQIVLLIILIILSGFFSASETALMALSRIRVRHMVDEEV
                     KGAKLLEKLLEDPNKLLGGILIGNNIVNIGASSLASTLAFRIFGDGGVALATGVMTIL
                     VLIFGEITPKSLAKQNAEKVSLAVAPIINIVVTVFKPLVWLFSSVSSLIVRLLGGDLN
                     KSEPFITEEELKTMVDVSEEEGVLEVEEKEMIFNVFEFGDLQVKDIMVQRVNVIAVNV
                     TATYEEVLKVIKEQQFSRIPVYNESIDDIVGILNVKDLILLDSDEENFNVTEYMREPF
                     RTFEFKKITELFAEMKTTRNHIAVVLDEYGGTVGIVTIEDLIEEIVGDIEDEYDERDK
                     DIEVIKENEFVVDGSTRLSDLSDMIGFEIESEEFDSIGGYVIGSLGRMPKVGEEIKTN
                     DFRVIVEELDRNRVKKVRVYTYKREKEES"
     gene            20908..21339
                     /locus_tag="K8M77_000583"
     CDS             20908..21339
                     /locus_tag="K8M77_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBI7085104.1"
                     /translation="MLSLALEEAEKAREKGEVPVGAVIVKNGEIIARAHNLKETLKDP
                     TAHAEILAIREACNKLNNWRLHGCEMYVTLEPCPMCAGAILQSRLSKIYIGTFDDTTG
                     AAGSVVNILQNHNLNYFLEVVWENDDKCSKILTNFFKDRRG"
     gene            21402..21860
                     /locus_tag="K8M77_000584"
     CDS             21402..21860
                     /locus_tag="K8M77_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085105.1"
                     /translation="MSKLDMMVGYEMNFYEKKFEENIKKLSYYMHRCFNETIGEKVAG
                     IAIEENLLYAWSYYFVTIADEDPKMGDRIMMGLINHFNTLLEDENVRINGKEGAKLMI
                     DPSFCRVWFGTSLNVMLVKKYGEEDYKEVFNYIKDNYLQKECKIRSLKVS"
     gene            22118..22780
                     /locus_tag="K8M77_000585"
     CDS             22118..22780
                     /locus_tag="K8M77_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBI7085106.1"
                     /translation="MKEDQFNIETLIVAILVMLTIINIDVVHVTEAQSVKIEIETEED
                     IPIVSDVFITVDDAKKWAQGKGATKEFVELADLYWEYSNEHGDVNPALAYVQAAKETA
                     YGNFGGVLDASYHNPCGLKTNKGGSDKDPKAHMKFKSWNEGVKAHLDHLALYAGAEGY
                     PKAETKDPRHFASIFGKANSVIDLSKNWAPSPTYGEEILGMYNEMMEGAGNRDIEVMN
                     IF"
     gene            complement(22879..24351)
                     /locus_tag="K8M77_000586"
     CDS             complement(22879..24351)
                     /locus_tag="K8M77_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBI7085107.1"
                     /translation="MDNKNFQSKTGFNLENTYLTLPNIFFSEQNPKGSKNPKLIKFNT
                     SLAEELGLNEEILNSDFGLNIFAGNETFPGITPIAQAYAGHQFGHFTMLGDGRALLLG
                     EHVTKDGKRYDVQLKGSGRTIYSRGGDGKAALAPMLREYIISEGMHGLGIPTTRSLAV
                     VNTGEEVLRKRFEQGAILTRIASSHIRVGTFAYAAQWGTLEDLKSLADYTIKRHFPNI
                     ANSENKYILFLEEVINRQAELIVNWQSVGFIHGVMNTDNMVISGETIDYGPCAFMDTY
                     DTNTVFSSIDYAGRYAYGNQPNMALWNLARFSEALLPLLNPNLDEAVNIAKKSISNFS
                     KLYKKYWFNKMRAKLGLFTEKENDELLIEGLLSTMQKYEADFTNTFVSLTLNKFEDEK
                     VFSSDEFKTWYALWQNRLKEENRPKEEVRNLMMNNNPYIIPRNHLVEEALKNAEKGDF
                     TFMDNLLEALKNPYSYSKDLEKYTKLPEKSDIPYVTYCGT"
     gene            24680..25876
                     /gene="plc"
                     /locus_tag="K8M77_000587"
     CDS             24680..25876
                     /gene="plc"
                     /locus_tag="K8M77_000587"
                     /EC_number="3.1.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C"
                     /protein_id="HBI7085108.1"
                     /translation="MKRKICKALICATLVTSLWAGVSTKVYAWDGKIDGTGTHAMIVT
                     QGVSILENDMSKNEPESVRKNLEILKDNMHELQLGSTYPDYDKNAYDLYQDHFWDPDT
                     NNNFSKDNSWYLAYSIPDTGESQIRKFSALARYEWQRGNYKQATFYLGEAMHYFGDID
                     TPYHPANVTAVDSAGHVKFETFAEERKEQYKINTVGCKTNEDFYADILKNKDFNAWSK
                     EYARGFAKTGKSIYYSHASMSHSWDDWDYAAKVTLANSQKGTAGYIYRFLHDVSEGND
                     PSVGNNVKELVAYISTSGEKDAGTDDYMYFGIKTKDGKTQEWEMDNPGNDFMAGSKDT
                     YTFKLKDENLKIDDIQNMWIRKRKYTAFPDAYKPENIKVIANGKVVVDKDINEWISGN
                     STYNIK"
     gene            26064..26990
                     /locus_tag="K8M77_000588"
     CDS             26064..26990
                     /locus_tag="K8M77_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBI7085109.1"
                     /translation="MKVDIISGFLGAGKTTLIKKLLDTLVKAEKVAIVENEYGEVGID
                     GDLLKDRRIEVKEINSGCICCTIKGDFKQNILDIISNYRPDRIIIEPSGVANFSQVLE
                     SIKEAHIEGLRINMKITMVDAQNVHMYMKNFGDFYRSQLVNANTVILTRVEKLSDKEI
                     THVCNEIKTINNKANIITTELSKLSPERIIQVSEKKVENLIENINIKKPKRIGLRRVS
                     APEFFENWGVETPKTFEYSELVKILNEFQNNKHGEVLRAKGIIKSKDNKWFKFDFVPN
                     DISIVNYKSDYTGRVCVIGRNLNKDSIDKLFS"
     gene            27010..27594
                     /locus_tag="K8M77_000589"
     CDS             27010..27594
                     /locus_tag="K8M77_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085110.1"
                     /translation="MKKCSVDLITGFLSSGKTSFINHILDDFSKEEVLVIQCERGQEE
                     LINCRKNINVKKFYSMDELKEEDLIRSLKFYAPKRVFIECNGVSETKKFIDKVTNSKL
                     KEYLKIGIIVNLIDMTTFNMFLKNIPSMIIPNISLSNLIVLNKCNEISENALKEYKDM
                     IERFNHNAHIVNCIGKEGTNMNFNKERLINRMMG"
     gene            27597..28490
                     /locus_tag="K8M77_000590"
     CDS             27597..28490
                     /locus_tag="K8M77_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI7085111.1"
                     /translation="MELLNKFSIVFSSIVIEALPFILIGAVLASFMQVYISNNIFNKI
                     ISKNKLLGSIQAGIIGVFLPVCECATVPITKGLLNKKVPLNVAITYMLAAPIVNPLVI
                     LSTYYAFDGNIKVVLLRVGVGFSIAVIAGLLMLCLSGENNIFIDNSEGELQGKCLCGC
                     SEIDDNSSKLIRLLKHTSLEFYEIGKYFIVGATLAAIFQTFVPRDIIFYFENSAVLSI
                     IILMAFSFLISLCSEADAFVASTFMNRFSLGSITGFLIIGPMIDLKNTIMLFSIFKKS
                     FVIKLLFVVFSLCFIASALIS"
     gene            28503..29222
                     /locus_tag="K8M77_000591"
     CDS             28503..29222
                     /locus_tag="K8M77_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085112.1"
                     /translation="MRKFNFSEFIWFLILFCFEYLIVKMFMSEKIFLIISRDMKAYVI
                     LAIILLFLILVIQFFNIFKTSTRRSFKGGYLIFIFALVILNIANGLNINQVSLNLKGV
                     KLYHDRHKESEHNHLHSNEKLNSNEALVFTEKNFHSSLEELILHLEDFVGKEISIEGL
                     LYVSELYEKEEFAITQIDMTCCIADSVYLGVLCKGDLPDLKNGSEIKVKGLVESKKIK
                     NSAGEEIEIPFINVFYTDTLQ"
     gene            29489..30277
                     /locus_tag="K8M77_000592"
     CDS             29489..30277
                     /locus_tag="K8M77_000592"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBI7085113.1"
                     /translation="MNPVAFSIGSFEVRWYGIIIALGILIAMTLVSINAKKKNLNFDV
                     ILDLFLWCFPFAIIGARAYYVLFELENYHSFWDMINIRQGGLAIHGGIIGAFLTAFIY
                     CKVKKVDFLAYADIVAPAFILAQGIGRWGNFFNQEAHGGQVTNEFISKFPEFIQRGMY
                     INGAYYHPTFLYESIWDIFVAILLMIILYNITDRYKGVVISAYISLYSLGRFFIEGLR
                     TDSLYFMNIRVAQLVSLLGIIIGIVAIIIIVSRGKEKGKGIFIN"
     gene            30527..30616
                     /locus_tag="K8M77_000593"
     tRNA            30527..30616
                     /locus_tag="K8M77_000593"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30561..30563,aa:Ser,seq:gga)
     gene            30619..30883
                     /gene="ffs"
                     /locus_tag="K8M77_000594"
     ncRNA           30619..30883
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="K8M77_000594"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            31048..31551
                     /locus_tag="K8M77_000595"
     CDS             31048..31551
                     /locus_tag="K8M77_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085114.1"
                     /translation="MVRKLLVGILWVTLSLFVLGCSTSGEKNTINIPIEKSMEEEKHK
                     DEEEYIDKISSTIVNMSVDGSIKNMRTVFETGKLNNTMIEQLEISNNFFKCLIEDLKG
                     VNVPEKFASYNDDIIDNLNKAKSSLEVMKNSHDEETIKSNAKIFIESNKGVSNLVDKI
                     LVYYAGC"
     gene            31988..33274
                     /gene="brnQ"
                     /locus_tag="K8M77_000596"
     CDS             31988..33274
                     /gene="brnQ"
                     /locus_tag="K8M77_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBI7085115.1"
                     /translation="MNKKKDILVIGFALFSIFFGAGNLIFPPYIGLTSGSEWLISFLG
                     FIISDVGIIFLSIVAVSKAGSFQGVVGRAGKKFGLSLEILMMLCLGPILVVPRTAATT
                     FEMSISPLLGNINPYLFSVIFFLIVFVLTIKPNKVMDIIGKVLTPLLLISLAVLIIKG
                     IINPIGELEKVNSGKLFMTGITQGYQTMDALGTGGIVALVMASFASKGYKDKKENRIF
                     TIKSALIACIGLAIVYGGLTFLGATSSTLYDSSISQTTLLMNITNAILGSTGTIMLAI
                     VIGLACLTTAVGLASVTAKYFEDVSNKKLKYKYIVIAICVFSALASNLGVDKIIEIAV
                     PVLSLIYPVSILLVVMNIFEKFIPNDMVVKGATYATLLISLLTVIDSLGIKIEFVHKL
                     PLANLGFNWVVPAIVGGLIVGLIFRKREVVSLEETC"
     gene            33576..34379
                     /locus_tag="K8M77_000597"
     CDS             33576..34379
                     /locus_tag="K8M77_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085116.1"
                     /translation="MKYYFKNEFKRALFGKNAIISFSITLGILIAKFMKEFGFESIKY
                     ISQYPTFDAIDALLIVMSSNIVIVFPLLATIIFSDSYLQEKESGFTKFIYTRMPLKKY
                     VSIKVIVNSISSALVAGASSLFMLIFLVVAVGIRNPQIASYTNNINGPLSYLLDSRDT
                     RLIYALIIILLFSIAYMVTATLALGISAWVKNRYLVLITPFFYYIFCGTIVERLGINR
                     IFDFHLARVISLKRSMTTLPNVVIYPLLIFIVGVALFYIGVVIKNEKDL"
     gene            complement(34602..35006)
                     /locus_tag="K8M77_000598"
     CDS             complement(34602..35006)
                     /locus_tag="K8M77_000598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085117.1"
                     /translation="MNKNLLLSIAILIPFNIIIKIKERALFLKIKLNTINKIEIKTKY
                     KITKILVINTYDRGVKLSVTSLIVSSAKARFTLFELVEKKYSLHELLKLIKLPISIDI
                     IFIAIRNIIEKIKAVSIFAIYISCLEANLILK"
BASE COUNT        13362 a         3822 c         5940 g        12058 t
ORIGIN      
        1 tgaagaggct cccctgctaa gggagtaggt cgggcaaccg gcgcccgggt tcaaatcccg
       61 gattctccgc caaagcatct agttttacta ggtgctttta tttatgttta atgtaaaata
      121 aaaataattt gaagagacat ttactttata gagtatttta gataaacttc tttatgtaat
      181 taataagtat tagaaaaaag atttaagcaa aatattttta taatttatta aataaaaaat
      241 atattaatat aaaatttata gataatataa ttttgtttgt agttaaaaat gtataattta
      301 attatatgaa ttttattggg aggagatatt ttgaaaggat catcagggcc agttagagct
      361 aaaaaaccaa aaagatcaac agcatataca ctatcgttat atttaggatg gagtagtatt
      421 ttcgcagcac tatacttttt tattgtagga ttaccttttt caattcctgg tattattgct
      481 ggttacatag cttttaaaaa gcacaatgaa ggtaaattac cattaatatt aaatataata
      541 ggatttcttt taacagtatt tacttgggta ttatttatga agataccatt aaaataatta
      601 ttaagaaaaa agatactata atggaataaa aaatagtatc ttttttataa aaagtagttg
      661 acatataaaa tgaaaatgat ataataataa atgtgtcaaa catggagaga tggtcgagtt
      721 ggtttaaggc accggtcttg aaaaccggcg tgcgtgtgag cgtaccatgg gttcgaatcc
      781 cattctctcc gccacaagtg gagaattact caagtggctg aagaggctcc cctgctaagg
      841 gagtaggtcg ggcaaccggc gcccgggttc aaatcccgga ttctccgcca aaagcatcta
      901 ggtttactag atgctttttt attttactta atttttattt cttttagaaa agtcatgttc
      961 tctaagacat tccttaataa atagtttaag cttttcatta tcttcattta gaagttcaat
     1021 aaaagcttct ttaaaaacct ttttaaaaaa ttccttaagt aatatataaa gaataaaagt
     1081 aaaaaatata aaataaaaaa atatatccat atagatttct cccttcttaa ttaaaaatac
     1141 tataccataa aattgaaatt aagttattat aagtaattag gataaagagt ttttatgaaa
     1201 tttgactaat taacatagta ttattcctaa gaatattcct aagaaaagtg atttgaatta
     1261 gatttatttt caataaagtt taaattattt atcacagagc atatattaat ccaagaaaaa
     1321 tataaatata tatgaagcag tattttatgg agttatgaat gaaagttgaa aaactaaatg
     1381 atattaaaaa taattctaag gaaattattt ttaatagtaa ttctaaaata gtcttattta
     1441 gtgattgtca tagaggagat ggcacatata aagatagttt agctcctaat atcaacattt
     1501 acctgtcggc tataaggtat tattataaaa ataaatttac ttatatagag gttggagatg
     1561 gcgatgaatt atggaagttt aaaaatatac aagagatata tgatatgaat tatgagattt
     1621 atgaagtatt agaaaaattt aaagaaaaaa aaagacttta tatgatatat ggaaatcatg
     1681 ataaagataa gagtaagata aaatttttaa ggaaaaacaa gaggagaaat aggtttaatc
     1741 actctgcttc agatttttat agtactttaa atatatatga atctttagta ctagtgcatg
     1801 aagaaagtaa aaaagatttt tttgtaattc atggtcatca aatagacttt ttaaataatg
     1861 aattagcatt tttatcaaaa tttttagtga gatatgtctg ggctatttta gaggctttta
     1921 tgggattcaa agatcctaca agtcctgcaa agagtaataa taaaagaaat ttatttgatg
     1981 agaaaataag taagtgggca gaggaaaata aaactagagt tattttagga catactcata
     2041 aaacgctatt tcctaagagc aggaatgaat caacatactt taatataggg tgctgtgtat
     2101 tacctagaac tattacagct atagaaattg agaggggcga gatttcactt ataaaatgga
     2161 caataaaggc tgatgaaaag ggatcacttt ttgttggaag agatattata ggtgggccta
     2221 ttagaataga aaattattaa agaaaaaact ctaatgttat acacaaaaaa acattagagt
     2281 tttgtatttt ctgttgataa tttcaatttt tatgttaata aattatattt taattatcat
     2341 tttttccctt agcttttact tctttatggt gtgctttttt ctctttttct tctctctctt
     2401 gctcttcaag gaaacatgcc attggattgc caagcttagt ttcaatttca gctgtagtat
     2461 ccataaattt tttgaaagta tcacctattt tcatttaaaa caccccttat tggttaataa
     2521 ccatctttat gattatttat gatttcacca ttattatata atgtattgta atctatttct
     2581 ataataaaat accttatata ttttttttgt catattgcta tttaaaaggc ataattaagt
     2641 ataaaatgtt attataagag gtaataattt ggaaatatat aaataaattt cgaggtgtaa
     2701 atatgggctt aaaaattata tatggaagag ctggtactgg taaaagtact ttttgtataa
     2761 atcaaataaa aaagaaaata aataattcac ctaccaataa attaatttta cttgtcccag
     2821 aacagtttac ttttcagact gagaataaag tattaaatgc cataggggaa aggtatgttt
     2881 taaatgctga ggttcttagt tttaaaagac ttgctcacaa tgtttttaat gagtgtggag
     2941 gagcaactag aactattatg ggggatgctg gtaaaagcat gcttattttt aaggtactag
     3001 aagatttagg ggataatatg actgtgttta aaaatgcctc tagacaaaag ggctttatag
     3061 acatagcatc aaaaacaata actgagttta aaaagtataa tgtcaataat gaagttttgg
     3121 atttaactat taatgaaata gaagatgaaa atctaaaaat gaagatggaa gaattaaagg
     3181 atgtttttaa tgaatttaac tcaagacttc atgagggata tgttgatgaa gaggatcaac
     3241 ttttactatt aaatgaaaag ttagatggat gttctttata tgatggagct gaaatttgga
     3301 tagatgagtt tagttccttt actccaaatc agttaagtgt tataggaaaa ttattaaaaa
     3361 aagctaaaag tgttaacata actttatcca tagatgaggt aaattcacct aaaggggaaa
     3421 gcgatctttt tgtagctaca aaaaatacag aaaaaaggct aatgaattta atacaagaag
     3481 aaggaattgc ttttaatgga tatattaatt taaatgagga tattccatat agattcaaag
     3541 aaaatagaga attagcgcat atagaaaggc aactctatgc atatcctttt aaacagtata
     3601 gaggggaaaa taatagttta aggctttata gagcaaataa taactatgat gaaattgagt
     3661 ttgtagctaa ggatatatta agacttgtta gagagaagca atacagattt aaagatattt
     3721 cagtaatatg cagggatgta gataattacg agaaggttat atcggcgatt tttgcagagt
     3781 atgagattcc ttattatata gataaaaaga tagatatagc tagtaatcca cttattgttt
     3841 ttataaactc agcagtagat ataataagca aaaattggac ttatgaaagt atgtttaaat
     3901 atcttaaaac tggacttatt aaagagttta gagggattga aggggcagag cttattgatg
     3961 aactagaaaa ctatgtattg gcctatggaa taaagggtaa aaagtggatg gaagaatggg
     4021 ttaactatag ttctagcatt ttaaaagaag aagagatatc agaggaaaat aagcaaagat
     4081 tagaaaggct aaatgatatt agagagacta tagtaactcc tttagatgag tttaacaaag
     4141 agtgcaaagg aaagaaaacc ttaaaggaat ttgccataat tttatatgaa tttttagact
     4201 ctaaattaga tattatggat accatagata aatatgttga gtatttcaag gaaaatgata
     4261 tggcaattga ggcaaaggaa tattctgagg taagagatat tttcatagat gttttagagc
     4321 aagcagttga tgttctagga aatgaagtta tgaatttaga tgaatttatg aaagtattaa
     4381 atattggatt atctcaatat gaaatgggtt taattcctgt agccttagat caagtaaaca
     4441 taggggatat aacaagaata aaaagtagag gaacaaaggc tttatacatt ataggagtaa
     4501 atgatggggt gctcccttca gctagcaaag aagaaggaat attatcagat aatgatagag
     4561 aaattttact tgaaaaaggg atttcactag cttcagatac tagaacaaaa atctttgaag
     4621 agcaattttt agtttataca gcctttacca tagcagagga atacttagtt gttacttatc
     4681 ctttagcaga ttttgaaggt aaatctcaaa gaccttctat tatagttcat agacttaaaa
     4741 agatacttcc aaatgtaaag gaagaaagtg aaggctttaa attagtaaat gataagtatg
     4801 ataagatttc tgctaaaatt cctactttaa atgagcttat gatagcaatt agaaaaaatt
     4861 atgatggtgc tgagatagag gactattgga aatatgttta tgattggtat ttaagagaac
     4921 ctaagtggaa agaaagaata gagtatgtaa ggaaggggct tgaatacact aatttagaaa
     4981 ataatatttc aaaagaaaag gctaaaaagc tttatgaaga taataaaaat aaaatttcac
     5041 taagcgtttc aagattagag agatatgccc agtgtccatt tgcttactat atacaatatg
     5101 gattaaaagc taaagacaga aaaatatatg agtttacagc acctgattta ggtagcttta
     5161 tgcatgaaat attagatgag tttaccaatg aaattaaaga aaaagattta aaatggagtg
     5221 atttaagcaa ggagaattgc agaaacataa taaattcttt agttgataat caggttaaaa
     5281 acaataagtc atccatatta aatagttcaa agagatattc ttattttaca gatagattta
     5341 agagaattct tacaaaatca gttatggtaa ttagtgaaca gatgaagaga agtgattttg
     5401 aaatttataa aaatgaatta gcctttggat tttctaaaga tgtgaattca ataaaactag
     5461 accttccttc aggagaaagt ttttatttaa atggaagaat agatagaatt gataaattaa
     5521 atttagatgg tgaaacctat ttaagaatta tagattataa aactggctct aaaaagtttg
     5581 atttaaataa attttataat ggacttcaaa tgcaactttt agtttattta gatgccctta
     5641 taaataattc agaaaatata gttgaaaatc aagctatgcc aggggcaata ctttatttta
     5701 gaatagatga ccctatatta aagtctaagg gagatttaac agaagaagaa ataaaaagtg
     5761 agattttaaa agagttaaaa ttagaaggtc ttttactaga cgacgtaaaa gttgtcaagg
     5821 caatggataa tactttagaa cctggaacac actcccttat aatacctgca aacatgaaaa
     5881 aggcagggga tttagggaaa aataaagctt taataaccat ggagcagttt gaattattaa
     5941 gaaagtatgt aaatgaaaaa atggttgaga tatgccaaaa tatgatagaa ggtaaaattg
     6001 atatagaacc atgtaaagaa aataaaaata tagtgtgtga ttattgtaat tactcacata
     6061 tatgccagtt tgattctagt ttagaagaca atagatacaa agttattcca aagaaaaaag
     6121 atgaagacat atggaagagt ataaatgaaa aagtaggtgg tgaagtaaat ggggactaaa
     6181 tggactgaag aacaagaatt agctataaat actagaaaat gcaacctttt agtagcggct
     6241 gcggctggct caggaaaaac tgctgtatta gttgaaagaa ttataaaaat gataacagag
     6301 ggtgaaaatc ctgttgatat agataagtta ctagttgtta catttactaa tgctgctgcc
     6361 tctgaaatga gagagagaat aggtgatgcg atatctaaag ctttggagaa ggatcctagt
     6421 tctgaagtgc ttcaaagaca attagctctt ttaaatagag ctagtataac aactatgcat
     6481 tctttttgtt tagaagttat aaaaaataat ttccacttaa tagacttaga cccagggttt
     6541 agaataggag atcaaacaga atgtgaactt ataaagcaag atattttagc tgaccttttt
     6601 gaggatatgt atgctaagga tgatgagtgc tttaaagatt tagttgaggc ttatggtgga
     6661 agcaaaagtg atgataattt aaatagcata atattaaaat tctataattt tataatgagt
     6721 ggtccttggc cagaatcttg gttaaaggat aaggtagaag aatttaatat aaattctata
     6781 gaggaacttg aaggcaagaa gtggatagaa gtcttaaagg aaagtataat tttagactta
     6841 aataatgcat attcaatgct tactcaagct agggatatag ctgaaatggg tggaggactt
     6901 gagccttatt tagttaatat atatcctgaa ataattcaag ttgaagagct tagaattgct
     6961 ttaagtgaag gaatagtaaa gttttataat aagcttatgg gagcaagctt tggaagacta
     7021 aaatcagtta gaaaagctag tgttgatgat gaaaaagcct tagaaaaaac taaaagtctt
     7081 agagatgaaa gtaaaaaaat aatagaaaat ttaagggata atgtttttga aacttcctta
     7141 gaagaagctg ttttaggaat gaaaaagatg tatccattaa tgaaatgttt aagtggttta
     7201 ataattgaat tttctaatag gtatagagat aaaaaaagag aaaaggatat tttagatttt
     7261 aatgacttag agcatctttg cttagaaata ttaatagata aagatgaaga ggggaatata
     7321 aaaccttcac aggttgcctt agaatttaag gataaatttg aagaagtttt agttgatgaa
     7381 tatcaagata gtaataccat tcaagaaact attgtaggaa tggtatcaag aagagatgta
     7441 gaaaatccta atgtattcat ggttggagat gttaaacaaa gtatatataa gtttagacag
     7501 gcaaatccag aacttttctt agagaaatac attaattata gagagtttga agattctaat
     7561 agaaaaataa tgctttataa aaactttaga agtagagaag aaattataaa tggagtaaac
     7621 tatatcttta aaacattaat gagcaatact gtaggagagc ttgaatatga tgaaaaagag
     7681 gctttaaacc taggggcttc ctatggggag ttaaatgagg aaaatgtaga gaaagaatat
     7741 atagatgaaa tagaaaatct aaaggtcgct ggtgatatag agctaaacat attaaataaa
     7801 gcaggtaaca aggattatag tgatgaggat gaactaggag aagaggaaga agatttagat
     7861 agcatacagt tagaagctag aataattggc aaaaagatta atgagctaat gaatcctgaa
     7921 gatggaagtc actatatggt ttttgataag gatttaggaa aatatagaaa gattaaatac
     7981 aaggatattg ttatcctgct tagagcaact aaaaattggg cagatacctt tgttgatgag
     8041 ttagggactt atggaatacc tgtttatgct gacacaggaa caggatattt tcaaactatt
     8101 gaaattagaa ctatattagc cttattacac ataatagata atccaatgca ggatatttat
     8161 atattatctt ccttacgttc accaatattt tcatttacat ctgaagaatt tgcagattta
     8221 agattattaa ataaagataa atacttcttt gaaataataa aggaagttgt agatggaatt
     8281 tatgatgaaa gtatatctaa agatttaaaa ggaaagtgta agtacttcct agactattta
     8341 aataaatgga gagaaaaggc agcatatatg ccaatagatg agtttatttg gttcttatat
     8401 tctgatacat catactatgg gtatgttgga acaatgccaa atggagttca acgtcaagca
     8461 aatttaagaa tactattcca aagagcaaag caatatgaat caactagttt taagggatta
     8521 tttaatttta taaacttcat aaataagctt aaaaagtctt ctggagatat gggaagtgca
     8581 aagattcttg gagaaaatga gaatgttgta aggataatga gtattcacaa gagtaaaggg
     8641 ctagagttcc cagttgttat attaggtgga acaggaaagc aatttaacaa gatggactta
     8701 agagaagata tactacttca tgaaacatta ggtataggaa caaattgtat agatgttaag
     8761 aagagaataa agtatgatac tcttcaaaag catgctataa agaaaaaatg tgaattagaa
     8821 gttttatcag aagaaatgag gatactttat gttgccttta caagagctaa ggaaaaatta
     8881 attataactg gtgctgtatc agatttagaa aagtcttgtg aaaactggtg caaagcttca
     8941 gcttcttcag aggataatag aataaaccct ggaaatgttt taaagggaaa gagttattta
     9001 gattggattg gcatggcttt aacaaagcac aaggatggag atgctataag aaatgttggt
     9061 aatggagata taactttaaa tttagatgat aaatctaatt ggagttttaa aagctttgat
     9121 agaagtgagc ttttagaaac aaacaataat aaaaaagaaa aaaataatat tgatattttt
     9181 gaaagtaata attggattga atctaaaaaa gatataaaag aagttataga aataagaaat
     9241 agactaggct ttaaatataa atatatagag tcttgtaata ctccttcaaa tatttctgta
     9301 accgaattaa agagagctca tcaagaagaa gagtttatgc aagaaagtta taatattatt
     9361 gataatgaaa gtagtgaaga aaataaaaaa gaaaaaataa aaagaaaacc aagatttatg
     9421 gaggaaagac aggaagaatt ctcagcagct aagaagggga ctataactca ctttgtaatg
     9481 caacatattg atttagataa ggtaacttat atagatgaaa taagagaaga agttcttaaa
     9541 atggttaaaa aagaactttt aacagaagaa gaaggaaaag tagtaaatgt atttaaaata
     9601 caaaaattct ttaaaagtga attaggacag agaatgttaa gctcatataa atctggcaaa
     9661 aaggtatata gagaactacc atttataact gagattccaa gcagtattat agaaaaaaat
     9721 cttgatccta aaatctatgg agaggaaaaa gtaagattgc aaggtattat agatgccttc
     9781 tttgaggaag aggatggata tgttctttta gattataaaa ctgattatgt taaagaggga
     9841 gaggaagagg ccttcataaa taaatacaaa attcaaatta acttatataa agacacatta
     9901 aataaaattt taggggaaga agttaaggaa gcttatctgt attccttcta tcttgaaaaa
     9961 gaattaaaaa taagcaagga ataatttcct tgcttatttt tctatgttta gattagctaa
    10021 gcttaattat atgaataaaa ttgcttacta aggtttagta agtcttcctt attgtttaac
    10081 tctggtgttt gcattacatt ttcaagaaga aaatttaata taacacctat attttttcca
    10141 ggtttaaaat taagttcggt aattaagtca tttcctgtta tggctaagtc cttaataaat
    10201 atgggttctt ttgaagctaa tatatcagaa actctatttt ttaatatatc tatacactca
    10261 gtatcatatt catccatata agataaatat gatttttgta aatctaataa atcaaagata
    10321 ttttcggttc caactgaatt tataagtagt tttagttttt ttcttgtcat attacatgaa
    10381 atactattta tattttctac taagttgcaa gtatcgttga ttgtattatt atcatattta
    10441 agtttcttta atatagttct gcacttctca actgcatttt cagaattaaa aaccttatag
    10501 aataatcctg ctaatcttat atgaagcttt ttaggaagtg aatttattaa tccataagta
    10561 tcatgaatat catcatataa atcatagcaa tttattagtt ttaaattctt aaattcctta
    10621 actaaaaagt cagaaaatcc tagctttcta agaagcataa gacctctatc aggctcattg
    10681 cttagaatga ttttagtaaa ttcactttga attctttcca tacttatatt ttttattaat
    10741 ttaaaattct ttcttataga ttcagcagtt aatttttcta ttttaaagtt taattgacaa
    10801 ctaaatctaa ttgctctaag cattcttaag gcatcttcag agaagcgttt atcaggatct
    10861 ccaacgcatc ttattaattt gttttttaga tcttcttttc cattaaaaca atccttaaat
    10921 cctaaatatg ggctatatgc cattgcattt atagtaaaat ctcttcttga taaatcttct
    10981 tctatattag aaacgaactt tacgtcttta ggctttctat tatttaaata ttccccatct
    11041 accctataag tagttacttc ataaggagtt ttgtcgatta aaactgttac tgttccatgt
    11101 ttaataccag tagggatagt tttatcaaaa agctcttcta ttttttcagg gaatgcattg
    11161 gttgtaatat cataatcttg aggtttttta tttaataaag aatctcttac acaaccacca
    11221 actataaagg cttcaaaacc attagagtca aatttctcta agatatattg gacattgtta
    11281 ggtaatttca ttgaactgtc acctcctagt agttatttta tcaattaata taaaatttag
    11341 ctataaatag agtacttatt tgtttacaaa aaataataat atgcaataat taaaatagag
    11401 attaaaatgg aaagaaagga tagacttatg gaaataccgg taaaaaaaat aggtgagttc
    11461 gttaaaggag aaagagtcga aggttttttt cttttaaaat cagttacttt aaaaactact
    11521 aattctggtg gaaaaaaata tttagattta gttttaagtg ataaaactgg tgatatatta
    11581 ggtaaagtat gggagattaa acctgagcat gaagagttaa aaagcaatac agtatttaag
    11641 attagaggta cggtgaatag ttggcaggga actttacagt taaaagtgga acatgctagc
    11701 aaagttactg aagaagataa tataaactta gatgattttg ttcagagtgc gccatatact
    11761 agcgatgaaa tgtttaatac tataaaagat actgttgata atatgaatga tatagatata
    11821 aagaacattc taaatagagt tttagagatt aataaggata aacttatgta ctatccagca
    11881 gcaaagtcaa atcatcactc tataaagggt ggacttttat atcacataac aactatgtta
    11941 aaattagctg agaatatttg tgtaatatat gatttcttaa acagagattt agtttatgca
    12001 ggggtaatac ttcatgatat ggctaaaatc aaagagatgt cttctaatga aatgggaata
    12061 gttgatgaat atactttaga aggaacttta cttgggcata ttactcaagg aataaaggaa
    12121 attgaggtaa taggaaaaga agttaatgct gatagtaaga ttataatgtt acttcaacat
    12181 atggtcttat cacatcacta tgagcctgaa tacggaagtc ctgttaagcc tcttatacca
    12241 gaagctgaga tgttacatta tttagatgta atggatgcta gaatgtacga tatgaaaaag
    12301 gcaattaaag aaactaagga aaatgaattc tcagaaagaa tttggtcttt agatagtaga
    12361 agaatataca atcacggaat gtatgaaaaa aataactaaa aaacagctcg taatgagctg
    12421 ttttttatgt tagtaaatta ttatttttga accattttgt acattatcat atagccattt
    12481 agaatcttca actgataatc ttatgcatcc gtgggaagct ggcaccccta aagttggatc
    12541 tgatactgta gttttatcag aattaaatgg gattgagtga aataaatagt tacccataaa
    12601 ttgaacatag tattttccac cttgtccgta tttaggtgag aaaaaccaag gagctctagt
    12661 ttgtacagta aatattccta caggagtttc ctttccttca atacctgttg aacaagtaaa
    12721 tgtcttatct aaagtccaat cattttttga gccttcatat acataagttt tttgttcagc
    12781 tatattcacg aaaaccaaat aattagtgtt actagttata tcaaattgat ttattgtatc
    12841 ttcatttaat tttttataat attccataac aggagcagtt ctagctgtgg ctttgtctat
    12901 attttcttga ctataagctt caagatgctc caatctaaga ttttctaatt catctctttt
    12961 ttcctgtaat tttggatcat caggtagaga attgatttta gaatctaaat actctattcc
    13021 ttttgaatat ttttcattag atatatattc atctacagtt tctaaaatag gttctcttat
    13081 tttatctaag caagtttctt cacattctag tgcttgagct ttacaatctg aaagattatg
    13141 tacactttta aagtagttta aagcttctaa ataattttct tcatctagct tagaaattcc
    13201 aatatcaaat gagtcctttg aatcttttat tagaggaatt gtgtttttaa aattaaaaat
    13261 ttcctcatta gacgtattta atgataatag tccatttaaa tcttttacag tttcttcttc
    13321 agatatatct ccagaataat actcattttc tatttctgat aatttttttg taattatatc
    13381 ttctgatgat ttaataaaag tttttttcaa tataatactt tttgttccat tattaaaaca
    13441 ataggcggca tcctcataat tatttgatga taaatcctct ttaaatttgt ttaaattatt
    13501 tgagtatgag aaaataaagt atgaaacaac tataattaat aacaagaata ttgataaaga
    13561 aattgaaatt aaaagttttt tatttttcat attttacctc gattaaattt agtaatttaa
    13621 tttaattatg gacaataata ccatttataa tcattatagg atatttttac ttgttaataa
    13681 attacaacta ttgtattttt tttgtgaaac agtcataata ttagtataaa gtaatagttt
    13741 taacaaaata ttaacagttt ttatgtaagg cggtgttgag catgaagaaa gttcatgaaa
    13801 gatttttaga atatgtaaaa gtagatacta aatcagatga aacaacaagg gttactccta
    13861 gtacaaaagg tcaattagaa ttaggaaaaa tccttgcaga ggaattaaag aaaatcggag
    13921 tagatgaagt aagaataagt gataaaggat atgtatatgc ttgtttaaag agtaattgtg
    13981 ataaggatat tccgaaaata ggatttattt cacatatgga tactgcacca gatatgagtg
    14041 gaaaaaatgt taatcctaaa attgttgaaa attatgatgg taaagatatt gaacttggaa
    14101 atggatatac attatcacca agtttttcac cagaacttcc aatgtataaa ggtcaaactt
    14161 taataactac tgatggaact actcttttag gtgctgatga taaggctggg gtagcagaaa
    14221 taataacagc tattgaatat ttaataaatc atccagaaat aaaacatggt gatattaaaa
    14281 taggatttac tccagatgaa gaaattggag aaggcgcaga tcattttgat gttgaaggct
    14341 ttggagcaga ttttgcttac acactagatg gtggaagaat aggtgaatta gaatatgaaa
    14401 actttaatgc tgcaagtgct aaggttgaaa taataggtaa aaatgttcac ccaggaagtg
    14461 ctaaaggaaa aatgattaac tctattttag ttgctcatga atttgtttct atgcttcctt
    14521 taaatgaagt tccagaaaaa acagaaggat atgaaggttt ctcattctta ttagatatag
    14581 aaggtgaagt agaaaaaact tcattatcat ttataataag agattttgat aaagaaggat
    14641 ttaaaaatag aaaagaaaga tttaatgaaa tagctaatga attaaataaa aaatatggag
    14701 aaggtactgt tacagtaact ttaaaagatc aatacatgaa tatgaaggaa atgatagaac
    14761 ctagaatgca tattgtagaa actgctgaaa aagcaatgaa acaatgtgga attgagccaa
    14821 tcaaaaagcc tataagagga ggtactgatg gggcaagatt atcatttatg ggactaccaa
    14881 caccaaatat atttactggt ggagaaaact tccatggaag atatgaatat atatcagtaa
    14941 attcaatgga aaaagctgtt gaagtaatac tgaatataat aaaaatttat gctgaaaaat
    15001 aagattaaat aaaacaaacc tataaatata tttagataat taatcaaaat atatttatag
    15061 gttttaattt tttatttatt ttaataattt ataataaact tactttatta aggttctttt
    15121 gtatctccta agaaaaagac agctaatgtt cctatacctg tatgagaacc tataactatt
    15181 cctatataat ctataagtat gtcttttaca ggatattttt ctctaatcat ttctgctaaa
    15241 gtttctgcat cttcaataca atcactatga gcaataaaaa ttgtttgctc attagggttt
    15301 acaatacgtt tttcaagctc ttggaataac atttttaaag atttttttct tcctttagcc
    15361 ttagcaaagt tatgaagttc acctgcatca tttacatata gcattggctt tatacttaat
    15421 aagctaccaa tagccgctgc tgtagatgaa attcttcctc ctctttttaa atgatctaag
    15481 ctatctactg taaagtaatg acataactta tttttgtttt cttctaccca agagtagatt
    15541 tcatctatag attttccatc ttttttcatt tgagcagcat aataaactag taatccttgg
    15601 cctaagcatg cagctctagt atctactatc tttatatcag catcaggata tttttctaat
    15661 aattcctctc tagctataag tgaactatta tatgttccac ttaaggctga tgaaaatgct
    15721 aagtataaaa tagaaattcc ttgtttaaca tattcttcaa aggcttcaac aaatctaaat
    15781 gagtttattt ggcttgttga tggcatgata ccttctctta atttattata gaaagctttt
    15841 ggagttaatt cgctatcaat attatcaata tattctgtat tatcaatatt acatataagt
    15901 cctaaataat taatatctaa atcttttacc atgctttttg gcagatcagc tgccgaatca
    15961 gtaaataaaa caaatttttt caaaataaat cttcctcctt ttcttaatag agttattcta
    16021 aataagtata atatttaata tgaaaaatat ctagggtata taatacaaga atgatccata
    16081 aatagtgtat ttaataataa tttaagtttt gggaagaata tgtaatatct tgatttatta
    16141 atgaatatat gatattataa gtctgttaat atgcggtggt agctcagctg gatagagtag
    16201 tcggctacga accgattggt cgggggttcg aatcctcttc gccgcaccat aagcttgtaa
    16261 ctttaagtta caagcttttt ttatttatat gtatataatt tcaatttaga ataaaaaaaa
    16321 ttacttaggt gaaaataatt ataaaatgag cttaattcat caagaatttt agtccgatta
    16381 tcttataaat aagataaaat aatatgctaa aggtgataat atgaattatg attttataat
    16441 tacgatttta gatgaacata atgaatcaag tattttatta gctgaaaagt taaagcctaa
    16501 agagataatt tatttatata aaaaggatga agaattaaag gttttaaatt cattgagaca
    16561 gttttattta aataaatttc caaattgcaa ttttagtgat gaaaaattag ggaaggatac
    16621 tatttcatcc atagaagaaa ttataaagag aatgaaaagc aaaaatacag ctatatgttt
    16681 aaatcaaggg aataaaaagg atattttaat aatgtatacc ctagcattaa aacataatat
    16741 agatggattc tttcttgata tacctaaaga ggagctttta aagttaaatt tagaaagtgt
    16801 acaatgtgaa aagtgtaatt tcgtagattt ggatgtagaa gacatcatag acagtatagg
    16861 ggcttctatt gttgtggatt caacagaaat ttcagagata aatattatag agaatatgac
    16921 taactatata gctagtaatt tagatctttg gaaaaagtat aaaataagat taagtgataa
    16981 ttctgttttt atccatgatg agtcaaatcc tagaagtata aaaatagata aagaattatt
    17041 gagcagagaa gaagttttgc ttcttgataa aatacttaac tttttagaga aaaatggcca
    17101 aataaaagta aaagaattag agcaatgttt aaaggtaaca ttccaaaatg aatttataaa
    17161 aggatttatt tttaaaagtg gaacatggct tgaagtttta acaaaaaata ttattgaaga
    17221 aataaaaagc attgatgata taaaaagcgg tgttttattt ctttggaatg ataaagaatc
    17281 tagagttaaa aatgagctag atgttgttgc cataaaggat tcagtgctta tttgtgtttc
    17341 ttgtaaggat tcaaaaaaat atgatgaagt tgccttaaat gaattaaatg tttattctga
    17401 gcaattagga ggagaaaatg taataaaaat attagtagca acacaccctc caattaaatc
    17461 atctatttca aaaagggcta aagaaatggg aataaactta gttgtttttg atggcaataa
    17521 aaaagcattt aaagaagaat tagaaaaaat aataaaataa aattttttat ataggataaa
    17581 taaagaaaat agggagaaaa taaatttaaa ttctcctata atcttatttt ataaacaggg
    17641 ctgtatcaaa atataataaa acttacaaac atattccgag aattactaga tttctcttcg
    17701 tactgtttgt aagttttata aaagttattt tgatacacgc cctttttaac tatttatgac
    17761 caatagtttc acccatatta actttagttt caaaacctaa tttagtttgt tcaactatat
    17821 cactatctat ttttatagta tctttcttaa agaataagat agtagttgaa ccaccaaact
    17881 taaagtatcc tttttcctct ccttttttta ctctcttttc aggagaatat gtttgaagta
    17941 ttgtaccaac acaagtagca ccaacttcta catgaagaat ttcaccaaaa ttatcagagg
    18001 taaaaataga ccattctctt ttgttcttac agaaaagttc agatacatta ttaagagcta
    18061 taggattaac tgaataataa aaacctgaaa ttttcttgct ttcactacaa gttccactat
    18121 ctacgaagtg aaatctatga tagtcagttg ggcataatct tagtataaga caagttccac
    18181 cttcaaattc ttcagctact ttaggatcat ctattaattc ttttaaagaa tatgtatatc
    18241 cttttatctg aacaacttta tcaagatcta tattttcaaa tgcagataat ctgccatctc
    18301 caggagaaat taaaatattc tcactataat caataggtct agcttcagga gttaaggtcc
    18361 ttgcaaagaa atcattaaag ttttcaaatt catctatatt tttattgcaa agagacatat
    18421 caatattaaa ctcatcaata aaggctttaa tttttttctt acttaaagga gagtcacaaa
    18481 aatttccgta aagttttgag aacatcttct ttttaataaa taattctaag aagctttttc
    18541 ctataggaga tgcataagac caatttatat atttaagacc agctacttgc tcaacatcat
    18601 aagcttttgt tttcctgtta taaattttta tcataaatta tatctccctt taaaaattat
    18661 gttaaataca tatattatat tattatatat ataaattcaa cttgtaaaca agaaattaca
    18721 aatttatatt gttgattgaa taaaataagt ggtatatgat taaattgttt taagaataag
    18781 tgttggagtt ggtagtatga atagaactaa gaaagctatt tttgaagcag ctataaacgt
    18841 ttttgctact tcaggttata atggatcaac tgtagatgaa attgcatcta aagctaatgt
    18901 tgctaaggga acactttatt ataattttaa aagtaaagag gaaattttta attttgtaat
    18961 tagtaaaggc ttagaaattt ggcatgaaaa attaactgat atagaaaatt tagaagatga
    19021 gcctatagaa aaattaaaaa aactttttaa aatgcaattt gaattattat atgaaaatag
    19081 agcatttttt aaaatggtaa tgagtcagct ttggggaaaa gaaactaggc aagatgaact
    19141 aagaaataaa ataactgagt atattgaagg aatagagaga atattaaaag aagctatttc
    19201 aaaaaaacaa ataagagagt gtgatatttc cttactagct cacagtttat ttggaagtct
    19261 tatatcaaca tcactatatg aattaagtag agataaagag tttaatgtta ataaagttat
    19321 agatgagatt acaattaaca tcttagatgg tatagttata aaataagatt tgttgacata
    19381 tattagttaa agagttaaaa ttattataaa attcaaataa gtctccatag ttcaatggat
    19441 agaacaatcc cctcctaagg gataaatgta ggttcgattc ctactgggga taccatggtt
    19501 ttaactatga aggaaaaact ccatatttat atggagtttt ttaaatatta ttatttaata
    19561 tgaaattttt gtataatata ataattatat aggaaaataa aaaagaagaa aaatatatag
    19621 aataatagga ggggtttatt tggacccata tacgtttcaa attgtgcttc ttattatctt
    19681 gataatttta tcaggatttt tttcagcctc agaaacagct ttaatggctt taagtagaat
    19741 aagagtaagg catatggttg atgaagaggt aaaaggagct aagttattag aaaaattatt
    19801 agaagaccct aataaattat taggtggaat cttaatagga aataatatag ttaatatagg
    19861 tgcatcatca ttagcatcaa cacttgcttt taggatattt ggtgatggtg gagttgcctt
    19921 agctaccgga gttatgacaa tattagtatt aatctttgga gaaataacac ctaaatcctt
    19981 agctaagcaa aatgcagaga aggtttcttt agccgtagct cctataataa atatagtggt
    20041 tacagttttc aaacctcttg tttggttatt ttcttcagta tcatccctaa tagtaagatt
    20101 attaggtgga gatttaaata aaagtgaacc atttataaca gaggaagaat taaaaacaat
    20161 ggttgatgta agtgaagaag agggagttct tgaagttgaa gaaaaagaaa tgatattcaa
    20221 tgtttttgaa tttggagatc ttcaagtaaa agatataatg gttcaaaggg taaatgtcat
    20281 tgcagtaaat gttacagcta cctacgaaga agtattaaaa gtaataaaag aacaacaatt
    20341 ttcaagaata ccagtttata atgaatcaat agatgatatt gttggtattt taaatgttaa
    20401 agatttaatt cttttagata gtgatgagga aaatttcaat gtaacagaat acatgagaga
    20461 accttttaga acctttgaat ttaaaaagat aacagaacta tttgcagaga tgaaaacaac
    20521 tagaaatcac atagcagtag tgttagatga atatggtgga actgttggta ttgttactat
    20581 tgaggactta atagaagaaa tcgtaggaga tattgaagat gaatacgatg agagagataa
    20641 agatatagaa gttatcaaag aaaatgagtt cgtagtagat ggtagcacaa gactttctga
    20701 cttaagtgat atgataggat ttgaaataga atctgaggaa tttgattcta ttggaggata
    20761 tgtaataggt tctttaggaa gaatgcctaa agttggagag gaaattaaaa ctaatgactt
    20821 tagagttatt gttgaagaat tagatagaaa tagggtaaaa aaggttagag tttacacata
    20881 taaacgagaa aaagaggaga gttaagggtg cttagtttag ctttggaaga ggcagaaaaa
    20941 gcaagagaaa agggagaagt tcctgtagga gctgtaatag taaagaatgg agaaatcatt
    21001 gcaagggctc ataatttaaa ggagacctta aaagatccaa cagcccatgc agaaattctt
    21061 gccataaggg aagcttgtaa taaactaaat aattggagac ttcatggttg cgagatgtat
    21121 gttactttag agccttgtcc tatgtgtgct ggagctattc ttcaaagtag actttcaaaa
    21181 atttatattg gtacctttga tgatacaaca ggagcagctg gttctgtagt taatattctt
    21241 caaaatcata atctaaatta ttttcttgaa gtagtttggg aaaatgatga taaatgtagt
    21301 aaaatattaa caaatttttt caaagataga cgagggtaag taatgattta cttacccttt
    21361 ttatgttata ctgagaatac aaatattggt gggggtgtat tatgagcaaa ttagatatga
    21421 tggtaggata tgaaatgaat ttctatgaaa agaaatttga agaaaacata aagaaattat
    21481 cttactatat gcatagatgt tttaatgaaa ctataggaga aaaagtggca ggaatagcta
    21541 tagaagaaaa tcttttatat gcttggtcat attatttcgt tacaatagct gatgaagatc
    21601 ctaaaatggg tgacagaata atgatggggc ttataaatca ctttaataca ttattagaag
    21661 atgaaaatgt aagaataaat ggtaaagaag gggcaaaatt aatgatagat ccttctttct
    21721 gtagagtatg gtttggtaca agtttaaatg ttatgttagt taaaaaatat ggtgaagagg
    21781 actataaaga agttttcaac tatataaaag ataactactt acaaaaagaa tgcaaaataa
    21841 gatcattaaa ggtctcataa tattttttaa tcagtaagta aagctttact tactgatttt
    21901 tctttatgtg ctataattaa taaaaattta tagtgttttt atagatatta tatgctattt
    21961 tgtaggaact ttaattttaa tcttttggga agttattaat aaagagtaat gatttttagt
    22021 gagctcaagt tttgggctca ttttttttac ataaataaga ataggatagc atattattta
    22081 tagagaatat tatgtaatag tattaggagg atctattgtg aaggaagacc aatttaacat
    22141 agaaacttta atagtagcaa tattagtaat gttaacaatt ataaatatag atgtggttca
    22201 tgtaacagag gcgcagagcg taaaaataga aatagaaaca gaagaggata taccaatagt
    22261 ttcagatgtc tttataacag tagatgatgc taaaaaatgg gcacaaggga aaggagctac
    22321 taaggaattt gttgaacttg ctgatttata ttgggaatat tcaaatgaac atggagatgt
    22381 taatccagct ttagcgtatg tacaagcagc taaggaaact gcctatggta attttggagg
    22441 agtattagat gcttcatatc ataatccatg tgggttaaaa acaaataagg gtgggagtga
    22501 taaagaccca aaagctcata tgaaatttaa aagttggaat gaaggagtaa aggctcattt
    22561 ggatcactta gcattatatg caggggcaga agggtatcca aaggctgaaa caaaggatcc
    22621 tagacacttt gcttcaatat ttggaaaagc taattcagta attgatttaa gtaaaaattg
    22681 ggcaccaagt cctacctatg gagaagaaat tcttggaatg tacaatgaga tgatggaagg
    22741 ggcaggaaat agagacatag aggtaatgaa tatattttaa gttaggaaaa ttatatagag
    22801 atttaggtat aaaaattggg atataaaaag cttttgttta taagcttttt atatcccatt
    22861 aatttatata attaaatttt aagttccaca atatgttaca taaggaatgt cacttttctc
    22921 aggtaactta gtgtactttt ctaaatcttt agaataacta taaggattct ttagtgcttc
    22981 taatagatta tccataaaag taaaatcacc tttttcagca tttttaagag cttcttcaac
    23041 caagtgattt ctaggaatta tataaggatt attattcatc attaaattcc ttacttcttc
    23101 ctttggtcta ttttcttctt ttaatctatt ttgccaaaga gcataccaag ttttgaattc
    23161 atcactacta aatacttttt catcttcaaa tttattaaga gttaaagata caaaggtatt
    23221 agtaaaatct gcttcatatt tttgcattgt gcttaaaagc ccttcaatta gcaattcatc
    23281 attttctttt tccgtgaaaa gaccaagttt agctctcatt ttattgaacc aatatttttt
    23341 atataattta gaaaagtttg atatggactt tttagcaata ttaacagcct catctaggtt
    23401 agggtttaga agaggaagta gtgcttctga gaatctagct aaattccata aggccatatt
    23461 aggttgattt ccataggcat atctaccagc ataatcaatg gaactaaata ctgtgtttgt
    23521 atcataagta tccataaatg cacatggtcc ataatctata gtttctcctg agattaccat
    23581 attatcagtg ttcataaccc catgaatgaa tccaacactt tgccaattaa ctataagttc
    23641 agcttgacga tttattacct cttcaagaaa taaaatatat ttattttcac tattagctat
    23701 attaggaaag tgtcttttaa tagtatagtc agcaagactt ttaagatcct ctaaagtgcc
    23761 ccattgagct gcataagcaa aagttccaac tctaatgtga ctagaagcta ttcttgttaa
    23821 tatagcacct tgttcaaatc tttttcttaa aacttcctca ccagtattta ctacagcaag
    23881 gcttctagtt gtaggaattc caagaccatg cataccttca cttattatat attctctaag
    23941 cataggtgca agggcagcct ttccatctcc acctcttgaa tatattgttc taccagaccc
    24001 ttttaattga acatcatatc ttttaccatc tttagttaca tgttctccta ataagagtgc
    24061 tctaccatct cctagcattg taaaatggcc aaattggtga ccggcataag cctgtgcaat
    24121 tggggtaatt cctgggaaag tttcatttcc tgcaaatata ttaagtccaa aatcgctgtt
    24181 taaaatttct tcattaagcc caagttcttc agcaagagaa gtattaaact taataagttt
    24241 aggatttttt gaaccctttg gattttgttc actaaagaat atatttggaa gagttaaata
    24301 agtattttct aggttaaaac ctgtttttga ttgaaaattt ttattatcca tattaaaatc
    24361 ctttgcctta taatttattt caaattttat tccatccctt atattatgag taaaaattct
    24421 tattaaatta aaaaacaata tttaacttat tataacacta ataattgtaa attttcatat
    24481 taaaaataag tttaataatt tagagtgggt gagtttagat attttaaatt aaaatttgaa
    24541 ttttattaaa aaatatttaa aaaatattca aaagtttagt gaggttatgt taattatatg
    24601 gtataatttc aatgcgagtg ttaatcgtta tcaaaaaggg gagattaata cttgaaaaaa
    24661 attaacgggg gatataaaaa tgaaaagaaa gatttgtaag gcgcttattt gtgccacgct
    24721 agtaactagc ctatgggctg gggtatcaac taaagtctac gcttgggatg gaaaaattga
    24781 tggaacagga actcatgcta tgattgtaac tcaaggtgtt tcaatcttag aaaatgatat
    24841 gtccaaaaat gaaccagaaa gtgtaagaaa aaacttagag attttaaaag ataacatgca
    24901 tgagcttcaa ttaggttcta cttatccaga ttatgataag aatgcatatg atctatatca
    24961 agatcatttc tgggatcctg atacaaataa taatttctca aaggataata gttggtattt
    25021 agcttattct atacctgaca caggggaatc acaaataaga aaattttcag cattagctag
    25081 atatgaatgg caaagaggaa attataaaca agctacattc tatcttggag aagctatgca
    25141 ctattttgga gatatagata ctccatatca tcctgctaat gttactgccg ttgatagcgc
    25201 aggacatgtt aagtttgaga cttttgcaga agaaaggaaa gaacagtata aaataaacac
    25261 agtaggttgc aaaactaatg aggattttta tgctgatatc ttaaaaaaca aagattttaa
    25321 tgcatggtca aaagaatatg caagaggttt tgctaaaaca gggaaatcaa tatactatag
    25381 tcatgctagc atgagtcata gttgggatga ttgggattat gcagcaaagg taactttagc
    25441 taactctcaa aaaggaacag caggatatat ttatagattc ttacacgatg tatcagaggg
    25501 taatgatcca tcagttggaa ataatgtaaa agaactagta gcttacatat caactagtgg
    25561 tgaaaaagat gctggaacag atgactacat gtattttgga atcaaaacaa aggatggaaa
    25621 aactcaagaa tgggaaatgg acaacccagg aaatgatttt atggctggaa gcaaagacac
    25681 ttatactttc aaattaaaag atgaaaatct aaaaattgat gatatacaaa atatgtggat
    25741 tagaaaaaga aaatatacag cattcccaga tgcttataag ccagaaaaca taaaggtaat
    25801 agcaaatgga aaagttgtag ttgacaagga tataaatgag tggatttcag gaaattcaac
    25861 ttataatata aaataataaa agtaaaaaaa taattattgg ttttggtggt atttacaaaa
    25921 taaaagctta ggaaagataa agtctttctt aagcttttat tttacttatt tttagagatt
    25981 tagggattaa tactttaagt aataatcctt ttgttttaag aatataatca ataagaatat
    26041 ggtttctata ttaggaggat taaatgaaag tagatataat ttcaggattc ctaggagctg
    26101 gcaaaactac tttaataaaa aagcttttag ataccttagt aaaggctgaa aaggtagcta
    26161 ttgtggaaaa tgaatatgga gaagttggaa ttgatggaga tttacttaaa gatagaagaa
    26221 ttgaagtaaa agaaattaat tctgggtgta tatgttgcac cataaaggga gactttaagc
    26281 aaaatatttt agacataata tcaaattaca gaccagatag aatcataatt gaaccttcag
    26341 gagtagctaa tttttctcaa gttcttgaat ctataaaaga agctcatata gagggtttaa
    26401 gaataaatat gaaaataacc atggttgatg ctcaaaatgt tcatatgtat atgaaaaact
    26461 ttggggattt ttatagaagt caattagtaa atgcaaacac agtaatatta actagagtag
    26521 aaaaactttc tgataaggaa attactcatg tttgtaatga aataaaaaca ataaacaata
    26581 aggctaacat tataaccact gagttaagta agttaagccc agaaagaata attcaagttt
    26641 cagagaaaaa agttgaaaat ttaatagaaa atataaatat taaaaagcct aaaagaatag
    26701 gtctaagaag agtttctgca cctgagttct ttgaaaattg gggagtggag acaccaaaaa
    26761 catttgaata ttctgagtta gtaaaaattt taaatgaatt tcaaaataac aagcatggtg
    26821 aggttttaag ggcaaaggga ataataaaat ctaaagataa taagtggttt aaatttgatt
    26881 ttgtaccaaa tgatatttct attgttaatt ataaaagtga ttatacaggt agggtttgtg
    26941 ttataggaag aaatttaaat aaagatagca tagataaatt attttcttaa aaataaggga
    27001 gaggattatg tgaaaaaatg ttcagttgat ttaataacag gttttttatc atcaggaaaa
    27061 acatccttca taaatcacat tttagatgat ttttctaagg aggaggtttt agtaatacaa
    27121 tgtgagcgtg gacaggaaga attaataaat tgtaggaaaa atattaatgt aaaaaagttt
    27181 tatagcatgg atgaattaaa ggaagaggat ttaataagaa gcttaaagtt ttatgcgcct
    27241 aagagagttt ttattgagtg taatggggtt agtgaaacta aaaagtttat agataaagtt
    27301 acaaatagta agcttaagga atatttaaag attgggatta tagttaattt aattgatatg
    27361 acaactttta atatgttttt aaagaacata ccttcaatga taataccgaa catatcacta
    27421 agtaatctta ttgttttaaa taaatgtaat gaaataagtg aaaatgcttt aaaagaatat
    27481 aaagatatga tagaaagatt taatcataat gcccatatag ttaattgcat aggtaaggaa
    27541 ggaactaata tgaactttaa caaagaaaga cttataaaca ggatgatggg ataaacatgg
    27601 agcttttaaa taaattttct atagtctttt ctagtatagt aatagaagca ctgcctttta
    27661 tacttatagg ggcagtgcta gcttccttta tgcaagttta tatttcaaat aatattttta
    27721 ataaaatcat atctaaaaat aaattattag gatcaattca agctgggata ataggagttt
    27781 ttttacctgt ttgtgaatgt gccacagtgc ctataacaaa gggattatta aataaaaagg
    27841 tgcctttaaa tgtggctata acttatatgt tagctgctcc tatagttaat ccactggtta
    27901 tattatcaac atattatgcc tttgatggaa acataaaggt agtattgttg agagtgggcg
    27961 taggcttttc aatagctgtc atagctgggc tacttatgct ttgtttaagt ggagaaaata
    28021 atattttcat agataatagt gagggagaat tacaagggaa atgtctttgt ggatgttcag
    28081 aaatagatga taattctagt aaacttataa gacttttaaa gcatacatcc ttagagtttt
    28141 atgaaatagg aaaatatttt atagtgggag caactttagc agctatattt caaacctttg
    28201 taccaaggga tattattttt tattttgaaa atagtgcagt tttatcaata ataatcctta
    28261 tggctttttc attcttaatt tccttgtgtt cagaggcaga tgcctttgtt gctagtacct
    28321 ttatgaatag attttcttta ggatcaataa ctggattttt gattataggg cccatgatag
    28381 atttaaaaaa tacaataatg ttattttcta tatttaaaaa atcctttgtt attaaacttt
    28441 tatttgtagt cttttcactt tgcttcatag cttcagcttt gatatcttaa ggaggaacct
    28501 ttatgagaaa atttaatttt agtgaattca tatggtttct tatattattt tgctttgaat
    28561 atttaatagt aaaaatgttt atgagtgaaa agattttttt gattatatca agagatatga
    28621 aggcatatgt aattttggca ataatattat tatttttgat tttagttata caatttttca
    28681 atatttttaa aacctcaact agaaggagtt ttaagggtgg atatttaatt tttatttttg
    28741 ctttagttat tttaaatata gccaatggat taaatattaa tcaggtctcc ttaaacttaa
    28801 aaggggttaa gttatatcat gatagacata aagagagtga acataatcac ctgcactcta
    28861 atgaaaaatt aaatagtaat gaagctttag tatttacaga gaaaaatttt catagttcct
    28921 tagaagaatt aatacttcat ctagaagatt ttgtaggaaa agaaatttct atagaagggc
    28981 ttttatatgt tagtgaatta tatgaaaaag aagaatttgc aattactcaa atagatatga
    29041 cttgctgcat agctgattca gtgtacttag gagtattatg caagggcgat ttgccagatt
    29101 taaaaaatgg aagtgaaata aaagttaagg gattagttga atctaaaaag attaaaaata
    29161 gtgcaggaga agaaattgaa attccattta taaatgtatt ttacacagat acattacagt
    29221 agatttaatc aataaaaaat taaagagact ttttaataaa ttatcttatg tatgaatata
    29281 tcaataaaaa ttaatactag cttaaaacag gattctaaat ataaaactaa atagaaaaga
    29341 tataagctta ataataagac tgttatatta cagattttat tattaagttt ttttataata
    29401 tttttattta aatgcgaata ttacatattt acttaataat tttgctgtag tattattata
    29461 aaaaattaaa aggaatggga gaggttagat gaatccagta gcattttcaa ttggtagttt
    29521 tgaagtaaga tggtatggca taatcatagc attaggaatt ttaattgcaa tgaccttggt
    29581 aagtattaac gcaaagaaaa agaatttaaa ctttgatgtt atcttagatt tatttttatg
    29641 gtgctttcct tttgctatta taggggctag agcatattac gtgctttttg agcttgaaaa
    29701 ttatcatagc ttttgggata tgataaacat aagacaaggt ggcttagcaa ttcatggagg
    29761 aataatagga gctttcttaa ctgcgtttat ttattgtaag gttaaaaaag tagatttttt
    29821 agcctatgca gatattgttg ctccagcttt tatattagcc caaggaatag gtagatgggg
    29881 gaatttcttt aaccaagagg cgcatggtgg acaagtaact aatgagttta ttagtaaatt
    29941 tcctgagttt attcaaaggg gtatgtacat aaatggagca tattatcacc ctacattctt
    30001 atatgaaagt atatgggata tatttgtggc cattttattg atgataattt tatataatat
    30061 tacagataga tataagggag tagttatttc tgcttatata tctttatatt ctctaggaag
    30121 attctttatt gaagggctaa gaacagatag tttatatttt atgaatatta gagttgctca
    30181 attagttagc ttactaggaa ttattattgg aattgtggca ataataatta ttgtttcaag
    30241 aggaaaggaa aagggaaaag gtatatttat aaattagatt tatatgtggc tgtatcaatt
    30301 ttatttagaa gatattattt ttaattttct ttttaagctt tgatacagct catttttaag
    30361 tgattatatt aaaataaata taaggtaaaa gtattattta aatcaaaatt tctgatttat
    30421 actaatttta ttatataagt ataataatta taattaaatt ttcagtattg aattggttag
    30481 acttatatgt tagaatgaaa aagtattaca aattaatatt tattatggag agatgtccga
    30541 gtggtcgaag gagcacgcct ggaacgcgtg tgtaggggaa actctaccga gggttcaaat
    30601 ccctctctct ccgccatgcc gtgctagatg gggagttagc ggtgccctgt aacctgcaat
    30661 ccgctacagc aggatcgaag tcctcataga ggctctaact tgtggagtct gccctatgta
    30721 agtggtgttg agagttgggc cccacgcaat ggaaacctgt gaacctcgtc aggtccggaa
    30781 ggaagcagcg ataagcagtc attttcatgt gccgtggata cgtctggctt gagctaactg
    30841 cataggtaac gttcataagt tattgtcgaa gtgaggtgca cggttttatt atgtaattaa
    30901 acttttaaca gagtatgtta aaagtttttt ttattttgtt aaattatgtt ttctatatgt
    30961 ttacgaaaca ttaataaaaa cttttatact tgttgatata ataatgtaca tatgcttatg
    31021 ataaaatgta attttacgga ggaagctatg gtaagaaaac ttttggtagg aattctatgg
    31081 gtaactttat cgcttttcgt tcttggatgt agtacaagtg gtgaaaaaaa tacaatcaat
    31141 attcctatag aaaagagtat ggaagaagag aaacataaag acgaagaaga gtatatagat
    31201 aaaatttcat ctactattgt taatatgagt gtagatggtt ctataaaaaa tatgagaaca
    31261 gtatttgaaa ctggtaagtt aaataatact atgattgaac agttagaaat atcaaataat
    31321 ttttttaaat gtttaataga ggatcttaaa ggggtaaatg ttccagaaaa atttgcttca
    31381 tataatgatg atataataga taatttaaat aaggctaaat cctctttaga agttatgaag
    31441 aattctcatg atgaggaaac tataaaaagt aatgcaaaga tttttataga aagtaataag
    31501 ggcgtatcta acttagtaga taaaatatta gtttattatg ctggatgcta aaccaataat
    31561 attaaattgt cataaataaa ttaatattta aaagatattt tatgttattc ataggttttt
    31621 caaagttttt taatattata tcattaattc ctattgactt tttatgattt aacaattaaa
    31681 attctattaa aataataaat tggaatttgg agattattta atatgaaaaa tttaaactta
    31741 actagagctt caaaattaaa taccttttat tttagctatt atagttaagt ttgcgtttat
    31801 gatatataaa agactcatag atgcaaacta gggtttgtat ctatgatggc taagtttaaa
    31861 atgcatgtag agccacatag ataaacacta tgtggctttt tatttaaggc actaaggcca
    31921 catagtttta cactatgtgg cttttttata tgaaaaatat aaattaatta gattggggtt
    31981 gtaaaaaatg aacaaaaaga aagatatatt agtaatagga tttgccttat tctctatatt
    32041 ctttggggct ggaaatctaa tatttcctcc atacatagga ttaacatcag gaagtgaatg
    32101 gttaattagt tttttaggat ttataatatc agacgtagga ataatatttt tatccatagt
    32161 tgcagtttct aaggcaggat ctttccaagg tgttgttgga agagcaggta agaagtttgg
    32221 actttcctta gaaattttaa tgatgttatg cttaggacct atattagtag tgccaaggac
    32281 tgcagcaaca acatttgaaa tgagtatttc tccactttta ggaaatataa atccatattt
    32341 attttcagta atattctttt taatagtttt tgtgcttaca ataaagccta ataaggttat
    32401 ggatataatc ggaaaggttc taacaccttt attacttata tcattagcag tattaataat
    32461 aaaaggaatt ataaatccta ttggagaatt agaaaaagtt aattcaggca agctatttat
    32521 gactggaatt actcaaggat atcaaacaat ggatgctttg ggaactggag gaattgtagc
    32581 acttgttatg gcttcatttg caagtaaagg atataaggat aaaaaagaaa atagaatttt
    32641 tacaataaaa tctgccttaa ttgcatgtat tggactagct attgtttatg gaggattaac
    32701 attcttaggg gctacctctt caactctata tgatagcagt atttcacaaa caacattact
    32761 tatgaatata actaatgcaa ttttagggtc aacaggtact attatgttag caatagtaat
    32821 aggacttgct tgtcttacaa cggctgtagg attagcttct gttacagcta aatattttga
    32881 agatgtatct aataaaaaat taaaatacaa atatatagta atagcaatat gtgtatttag
    32941 tgcattagct tctaatttag gagtagataa aataatagaa attgcagtac ctgtactaag
    33001 tttaatttat ccagtttcta tattattagt agtaatgaat atatttgaaa aattcattcc
    33061 aaatgatatg gtggtaaagg gagctactta tgcaactctt ttaataagct tacttactgt
    33121 aattgatagc ttaggaataa aaattgaatt tgttcataag ttacctcttg caaaccttgg
    33181 atttaactgg gttgtcccag ctatagttgg tggcttaata gtagggttaa tatttagaaa
    33241 aagagaagtt gtatctttag aagaaacttg ttaaattatt ataaatctac gtaacatata
    33301 attggttaaa tagtaatata atgatggaaa aaataaagat atttataaaa tctatttcaa
    33361 ttttataaat atctttaatt attttataaa ataaaaatta aaaaaataaa aataaagtaa
    33421 aaataaatga aaaatatggt taaattttaa ttgatttaca aaaaagatat aaaagtataa
    33481 aatttttatt gaaaatatga tttatagggg gaataaacat gaaaaaatca ttaaaaatag
    33541 gattgaattt ttaggtaaaa ggatgtgttt taaatttgaa atattatttt aaaaacgaat
    33601 ttaaaagagc tttgtttggg aaaaatgcta ttatatcttt ttcaattaca ctaggtattt
    33661 taattgcgaa atttatgaaa gagtttggct ttgaatcaat aaagtatatt tcgcaatatc
    33721 caacttttga tgcaatagat gctttattaa ttgtaatgtc gagtaatata gttatagtat
    33781 ttccactatt agcaactatc atattttcag attcatactt acaagaaaaa gaaagtggat
    33841 ttacaaagtt tatttataca aggatgccat taaaaaaata tgtttctatt aaagttatag
    33901 taaatagtat ttctagtgca ttagttgcag gggcatcaag cctttttatg cttatttttt
    33961 tagtagttgc agttggtatt agaaatcctc agattgcttc ttatactaat aatataaatg
    34021 gaccattaag ttatttactt gattcgagag atacaagatt aatatatgct ttaataatta
    34081 ttttattatt tagtatagcg tatatggtta cagcaacatt agctttaggt atatcagctt
    34141 gggttaaaaa tagatattta gttttaatta ccccattctt ttattacatt ttttgtggaa
    34201 ctatagtaga aagattggga attaatagga tttttgattt tcatttagcg agagttatct
    34261 ctttgaaaag atctatgacc acccttccca atgtagtaat ttatccatta ttaatattta
    34321 ttgtaggagt agctttattt tatataggag tggttattaa aaatgaaaag gatttataat
    34381 tatatatcat ttaaaattca ctctacaaag ttattaatat tttttactat attagtaatt
    34441 tatcagtttt cattattatt atcattaatt tttgagggga aaagttatac tgtttatgat
    34501 tttatttggt ctaacttttc atatttaaca ttattttatt tcacaagttt aattttttta
    34561 attatgattt acaatatttt tgagaaaaag aatttttata attatttaag tattaaattt
    34621 gcttcaaggc aagaaatgta tatagcaaat atacttacag ctttaatttt ttctataata
    34681 ttccttatgg ctataaatat tatatctata ctaatgggaa gttttatgag ttttaataat
    34741 tcatggagtg aatacttttt ttcaacaagt tcaaataagg tgaatctggc ttttgctgag
    34801 gacactatta aacttgttac tgagagttta actcctctat catatgtatt tattactaat
    34861 atctttgtta ttttatactt agtttttatt tctattttat ttattgtatt taatttaatt
    34921 tttaaaaaaa gagctctttc ttttatttta ataattatat taaatggtat taatatggct
    34981 atggataata gtagattttt attcactaat aatatttaca tattaaattc taaagcaatt
    35041 gaagtaacta atggcactta tataatatca agattatgtt attggataat tttaatacta
    35101 ttagtttctt ttataggttt tatattgact aagaagaaag attgtaattt tggtgattag
    35161 atatgaaaaa atatttgaat at
//
LOCUS       DAEVGI010000013        32025 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEVGI010000013 DAEVGI010000000
VERSION     DAEVGI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 32025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32025
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.013"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            294..374
                     /locus_tag="K8M77_000599"
                     /pseudo
     CDS             294..374
                     /locus_tag="K8M77_000599"
                     /inference="COORDINATES: protein motif:HMM:NF012621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
     gene            1073..1654
                     /locus_tag="K8M77_000600"
     CDS             1073..1654
                     /locus_tag="K8M77_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085118.1"
                     /translation="MENLLKDYSNEEVLKALKIVTELKLEREQGDLPEFIRGRVSEVS
                     EDEFDLIQAYNYYMRELRRGSVLLSEEETEKCGFNSRLSIDVELKDLIIFLNTQPNIK
                     TLYSCSGHQFVRGQITFEGLLKFPQVDDFKIEYADERDETKVSFDIPERSYDYLNKLR
                     KLYNYFYLINYNNLKTLTRDEVSSIKIKENCTA"
     gene            complement(1791..4166)
                     /locus_tag="K8M77_000601"
     CDS             complement(1791..4166)
                     /locus_tag="K8M77_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HBI7085119.1"
                     /translation="MDNSIFFHIFNDDKNKLETKRIIKLSIFIVFSIFFILTLDLSYK
                     VLIRKNIEFIPNNSIPNFPLSLSLILGTMAYISSLIYYSSTKKDDFFIISLIYMNLSV
                     ELLITKGHNLIIFDKFIFIHAIFRIILLFYVAFNKKGISPLITKHKTISSVAVFLFSV
                     ITPMINYKIFSNKLFTKDIYFYATLMTIIIILYIIACIFLSKKSLDDCELIYSFIIAS
                     ILLIALRGLYWICEVLLPNITLLKTNNVVLLLTILSFLLAISGVFNEITTKNKKSSLL
                     QNELQVFYHLVEFNTSSSIILYDNKKKVIYTNKTIRERYCKSTELKDQLKEVEKLFVD
                     SIFIDDSEKNATKSLFNKGNWEGKIILKNDKIVSAYIQILNVENKNYFAVNLKDITEE
                     YTLTKNIKRNEQLLSCINNNVQDLIISVDNNGLITYVNDSVLETLNYTYEEIIGMPII
                     NLLGKNDEILNQLKLEDEEDSIKCKLVGKHSFVYVESIIRTLSDNNEIPYGKVIVAKN
                     LTSKKRLENLAIKFKEAKAYEQIRNEFFANISHELRTPLNIIYSTIQLLNSKHETNYV
                     NFNDFYGKYKQGLKINCYRMLRLINNLIDVSKIEVGFLKADFTNRDIVFLVENIVSLV
                     IPHSENKDINIIFDTNVEENIIKCDPVKIERLILNLLSNAIKFTQNHGKIFVDLNISK
                     DWVKISIKDNGIGIPKEMQASIFDRFVQADKSLKRRNEGSGIGLSIVKSIAELHDGKI
                     ELISDGIKGSEFIVWLPNVKLNYTEESNNLVDYITDDKNIELELSDIYEVH"
     gene            5001..5703
                     /locus_tag="K8M77_000602"
                     /pseudo
     CDS             5001..5703
                     /locus_tag="K8M77_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5863..6921
                     /locus_tag="K8M77_000603"
     CDS             5863..6921
                     /locus_tag="K8M77_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI7085120.1"
                     /translation="MNVVLKQRKRLDYLDISKAFLIISVVLCHSPFENAYYLYWFHMP
                     AFFIISGMLYKRGMNIKEQAIKFFVPYAIFSLIDINFNYIMYYHGNFSLSDLIHEIIK
                     YSYGGKATWGVFWFIPVMFVTKVVFNQLNKRLTSKKLAFLCILSYILAHIYSMSFIPS
                     SVTEITENQFVFWNLDAVLITLPYYALGYYITKLNIQRVFKETSTLILSSIGVVAMFI
                     LNSSMGIGYYLNIKYSYYKDPIFDILMPVTITIFILSLSYQISKFKCKKLFTIIGANS
                     LVIMYLHKQIATLFMDVFNYGYIMFTIIGVCLPLLMMVLINKSIVSKFLFNGDLTFYR
                     KIKDIKDIEPKDIIIDNK"
     gene            7104..7985
                     /locus_tag="K8M77_000604"
     CDS             7104..7985
                     /locus_tag="K8M77_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085121.1"
                     /translation="MTMQRKNFNNYKEVSLLIMLIPMLFILIDTLGRMNSKINNKNLF
                     EIIFNEIFLYREVIVKFIMAIVVIFVISKIKIIKEKFILGDFKIIIGYTTLGIISYKF
                     FGSEYGLSKSLDVYVMCIIAMIFILSGIREIISSIDNININIEEKSNFERLVYTRVKY
                     LGIIYFIIFSILIAIENLNSIMSSDFIVSLISNYNQHYNNMARVISVLGVVLIAALII
                     IIKKSFSNLNLCYEEYIYIIKHKTVTEEIKKDIDFVNLKVEANKKYINNKFKKLEDKK
                     TQVLLKGIFDFFKNNKH"
     gene            8014..10212
                     /locus_tag="K8M77_000605"
     CDS             8014..10212
                     /locus_tag="K8M77_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085122.1"
                     /translation="MDSKKSTNYDFILKTSIERYYDLLKNAEKAWETHAALTYIALRM
                     VVEDLLIKLEEKYEVNRDKEDNFKNTLNKLKEYVPSWIIEYGEYIRIKGEKSANKEID
                     SNLEENSTLLALKKVHEILIWYLKNVTKEMSLEADYSFITPESIKESECDLAIDKKVL
                     ENKEKFIISLEKQIKTNSDDEKKQEELEELLRNVKEEKKELTEKLEKKELELQEVLNS
                     FKELQVKLTTYIEKDQSAEESNNKYPDFYKGFLSLQGYELRVVYAFLKSLSLGSIFLN
                     KSKSIFLKSQRKEFLKFIEKEVEDLKKYSDGILKLKLYYILLKLTNTPVASVADKETF
                     DKSLDSIVEKAYFIIESRNGFNGKVDKLEAIINYYLIVIINSLSAEINHEEDNEGKVS
                     DIIYEVLKTIPLDERKIILNKMNFTDISEGAIKENIRLNQVEFLKAALDCGISESSEF
                     VIKSVFSIYVAFQKDFDFYEFRTRHDMLRAYIGPLLLVILLKEFKISRENLVPIFVME
                     ISISNKILRKERREKIYYNQLVQAWLEEKEKYDKILSEKDNLEKNLKEVQEARELLLT
                     NERKFVAEKDNTENAIKSQWDMFKEKALQSEEIKSFNSYEEYRRAVNQKNLIEVSEEY
                     SLKDRGIIKNFFSLDTWKYKLNKLEISKNIKVLEEKLIEEAKESGVFENELLTIRELE
                     KILEDKESGLEDIRVDLKENSVENEKLNRLISNSSKLMEKFKEDNFDFAK"
     gene            complement(10428..11243)
                     /locus_tag="K8M77_000606"
     CDS             complement(10428..11243)
                     /locus_tag="K8M77_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBI7085123.1"
                     /translation="MKFDLSKMYEKLLSWITTNGFKLVIGLILLYIGWKIINKVIKIL
                     ANIMKSRNVDPTLTSFAEAFSEVSLKVLLVITLMSYVGFDIAGLAALIASAGLAVGLA
                     LQGSLSNFAGGVIILLLRPFRVNDFIEASGYSGTVERITVFYTHLVTPDNKEILIPNG
                     TLANGSLINYSSKDTRRVDLIFSVGYDDDIIKVKNVLWDIINKNELILKTPEAFVGIS
                     QHAASSIDFTVRVWTKQEDYWKVHFSLLEEVKLRFDEENITIPYPQMDVNLTK"
     gene            complement(11850..12494)
                     /locus_tag="K8M77_000607"
     CDS             complement(11850..12494)
                     /locus_tag="K8M77_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HBI7085124.1"
                     /translation="MNSFMMDIKNNSNDLHAVAEKTGFLKRLLEGKASTESYAEYLYN
                     LYEVYNAIEVNLEKCKDNKVVKDFVLPEIYRAEAILKDLKFLLGENLNTMKPLASTRA
                     YVARINEIGETAPELLVAHAYTRYLADLFGGRTIYGMVKDLYKIDEEGLNYYKYETLS
                     DGPEMKGFVMNYHNKLNNIELNEEMKERFINEVANSYVYNIAISNELDFIRFNR"
     gene            12896..14119
                     /locus_tag="K8M77_000608"
     CDS             12896..14119
                     /locus_tag="K8M77_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBI7085125.1"
                     /translation="MRILHTADWHLGKNLEGLSRMDEQERFLEDFVHIVKDNNIDLVI
                     IAGDVYDSSNPPARAEKMFYDTLKKISANGERMTLVISGNHDNPERLVAAGPLARDHG
                     IIMVGTPKSIVEVGEYGRNKVLNSGEGFIELDINGEKSVILTVPYPSEKRLNEVLYGA
                     MDETCERIKSYSDRIKLLFDSLKGNFKEDTINLVVSHLFATGAENTGSERNIELGGSF
                     IVDKECFPEEAQYIALGHIHKPMILPGSNKKIRYAGSPLQYNKKEISFEKKCFIVDVK
                     ANEEANIDEIKFDVYKPIEVWKCDSIDEAIYKCEENKDRECWVYLEIKTDRYIREDEI
                     KAMKKLKGDIIEIIPKITLDEEENDERSFSEKSFEELFTDFYEKERGVKPEEDLLELL
                     LNIVKEEGDEENETN"
     gene            14106..17624
                     /locus_tag="K8M77_000609"
     CDS             14106..17624
                     /locus_tag="K8M77_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7085126.1"
                     /translation="MKPIKLEVKGLNSFIDKQVVEFDKLTERGLFGIFGPTGSGKSTI
                     LDGITLALYGDIARKSSNYINTNCDGVYVSYEFQITGNEVKRYRVDREFKRDNKSGGI
                     RNKSSKIIDITGGVENVLEEKAKAVTSKCEEIIGLKLDDFMRTVVLPQGKFSEFLKLE
                     GKERRNMLERLFNLRKYGDDLSSKLSLEIRKEKDKMNVLEGQLKGYEGVSEEALKAKE
                     EEINEINLSIKSKEELLNKIKKEFEEAEKVWNTQKELYDKRIEEESLVSRSEEIKSFK
                     ERVEISNKADKVIVFINNLEEILKEINKEDLKFNELNKKLEELTNLREENKLKFEEVS
                     KKKEEKLPDLRLKKEKLLESQKERDILFQIKADGVKLKEACKKIFEDRSKCDTKLNSI
                     EENEKRLNEELKEKEERKEELFVHEEFKNKINSGLFILSSYEGLDKQFNEIKSEEIEL
                     KKYIKNLTEDKEKSEKDLKVKIESLSKTRDKLESLLKKTPGDSNSILEKQIKLGEYRE
                     KLNKYKEIKNSLEESLKSKNNFEEKLKAFENQKLLLEKEVRELKEYINKVKVEELAHK
                     LRENLVDGECCPVCGSTHHELNKVEKINLEESNEKTTLLESKEEKLKELILEFSKIEA
                     TLEYENKKIEELNISIEEVGEVNEERLKSLEEEFNTLKEKVEEFTLKKENLEKDLEKL
                     KEEKNNLENIFNKAEVILCEKIVREKEIASKIKEVDKELKLKNSELNSIKNELKIEDI
                     KLENELILKKEKEKNLLEKEIRILRTQLEESNKIKDELREKRDALKEDYLSQKSLLDG
                     KVEVYREKERMIKGSLKGLIDEALPIEKIDIKGLLEDLQLEIDYIEKAYLNLSEEKEK
                     LEKAFNNMNQEVAVTKERVNSLNLRKENEEKKVNLALEEEGFKTILEVKEGILSKDEK
                     EKLKTLIEEYDNNLIKVRANIELLIKKLNGKSLTEEEWTRVLQEKNNTEKELKEVEEL
                     KIRLVTENESIKKKLEEQRDILHIKAKQEHKLALLSDLEKLFKGKKFVEFIAANQLKY
                     ISIEASKKLKDITNGVYGLEVDENGKFIIRDYKNGGAKRDASTLSGGETFLASLALAL
                     SLSSQIQLKGTAPLELFFLDEGFGTLDDNLLDVVMSSLERLHHERLSVGIISHVESIK
                     NRVPVKLILTPAEAGIGGSKVKIERS"
     gene            18028..19224
                     /locus_tag="K8M77_000610"
     CDS             18028..19224
                     /locus_tag="K8M77_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBI7085127.1"
                     /translation="MKILIINCGSSSLKYKLIDMVNEKDIIEGIIERIGLDQSRLVQK
                     NELREKYILEKEIKDHKEAIDIVLNTLVDSKVGVIESIDEITAVGHRVVHGGERYSSS
                     IIINEEVIKYLEECSKLAPLHNPANIIGIRACQSLMPNKEIVAVFDTAFHGTLPEKAY
                     IYAIDYALYKDHKIRKYGFHGTSHKYVSHKVAETMGKDIKDLKIITCHLGNGASISAV
                     KGGECIDTTMGFTPLAGIPMGTRSGNIDPSIIPFLVQECGYTIEEVSESLNKKSGVLG
                     ISGVSSDFRDIEDAASNGDKRAQLALDIFHYRIRAEIGSFIVNMGGVDVIVFTAGVGE
                     NSPETREECLKDLEFLGLTLDKEKNKVRGKLAEISQADSKIKAYVVPTNEELMIAKET
                     VELIGK"
     gene            19660..20481
                     /locus_tag="K8M77_000611"
     CDS             19660..20481
                     /locus_tag="K8M77_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HBI7085128.1"
                     /translation="MGFIVFCLIAYFVISNLSNKKDVSGNKANSNCPYCNAAFYLTEN
                     GDFNCSNCGRLFKYRDGIVYRQDERLPIVVELACTLFTILCKADGIVTKDEVSITKEL
                     LEKNFGLEDKDMKMAIDILNKSKSKPYNKTIINDLNNIFNNYDFSKIDAEDYKELILR
                     CAMIIAFCDDGEPSNNQNKILDDIVSIFNISATKYTNLLNYFRDNCIEKNKNNYYEIL
                     GVSEGASKEEIRAAFRKLSKLYHPDRYSSKDLPPEIIKEFEEKLAKIIEAYEALK"
     gene            complement(20730..21488)
                     /locus_tag="K8M77_000612"
     CDS             complement(20730..21488)
                     /locus_tag="K8M77_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085129.1"
                     /translation="MIFLKSILVDFFNCCEIPIRAVDNNMNILSEVGYSSFYDYIFNN
                     LNLNSKFERSYLKHDHNDSLIITFENNISFYSLFNYKSIDKDLIFIIGPLYLKEDLKN
                     SNDDLELYKIPLKTKSCLKYYNSLLKIILDEKLKDNFSISYSPYIGRAIDFIEKNYAE
                     EITIDSLCTEFKINKSYFCNIFKNETGQTFINFLNNYRIKKSKDLLKDLDLSLLDIAH
                     KVGFSNQSYYCTVFKKFTGLTPLKYREDNYKKGL"
     gene            21634..23865
                     /locus_tag="K8M77_000613"
     CDS             21634..23865
                     /locus_tag="K8M77_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HBI7085130.1"
                     /translation="MGRKKINKILGYALVTSMLVGVGADTVYAATNINSGSAIVRSEG
                     LRDGIYEANNVTSYVEEGNSTGENMARNAIKEKTKFKIENGKTLMTVYFNSSLYGFMN
                     NIEVRAGGEDLKIEENKDDKSITFEVPSPDTKVKIGLFITMMGRKVELFLVNDMNTVN
                     LLDEAPTINNAKDISVTQGDAIDLLSGVIGTDKEDSNLKVEISGDTSFIKDGKAEIPG
                     VYPITYKVTDSSGQFDEKTVNVTVNKKTTLGDGSYILKNTVQYVGQGNMETGNSMARK
                     VLSENSRIDISNGKNTVTLTFNSELYAFLKNFNVTVDGEKVEAEVNKDNRTIKFNIPD
                     LNSDIVVSTLVSMMGKEVSFKTTLNYDTAKKLENNLEDNNKPGNEDSENIPGGNENSG
                     NQDNSGNTNQGSNGSANEDKENESEGSSGEIVDANQLKNGIYNIKNDVSYIGDGNQDV
                     GNDMARKALSKNSKLEVKDDKKILTLKFNEEQFSFFKDFRITANGKDIIATPNEADRT
                     ISFEIPSLDADIVVTAFVSVMGRDVSFKTILNKGTLELVSGEDKPAIEEENKSEESNG
                     TSSSNNGSSANEGNSTVTENKVTKGKLYTIENKVVHKSQTGVDMARKYLNKISDLEEI
                     DGKTYLTLTFTGQEFMKDHKISVNGKDANYKVVSKNGDSIKLRFEIPNLDADIEVSLY
                     VIPMGRNVEFNVELLKDTKKFVKDFTLSSLPQTGSPIGGNSVALLGMAMMGASMFIRK
                     REE"
     gene            23928..24575
                     /locus_tag="K8M77_000614"
     CDS             23928..24575
                     /locus_tag="K8M77_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEAT domain-containing protein"
                     /protein_id="HBI7085131.1"
                     /translation="MRFLKPVLLVMLTLFISLTGVFGSVDVLANENLENGIYDVKNDV
                     YHDSDIGMSMARSYLNDTMQLKILGDEKEYTIGFSGTEYMENFRILVDGNDGNAEIVE
                     EDKENGTIKLKVKVPNKDSELEAKIYVGPMERDVQFKVIPKFDTLTLVEKLEEPKVEN
                     KESSSENLSDETGKSEDAISEATGLANNKNMFIYIGGGIIIVALIVSLVIFKRKK"
     gene            24577..25140
                     /locus_tag="K8M77_000615"
     CDS             24577..25140
                     /locus_tag="K8M77_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HBI7085132.1"
                     /translation="MRNKLAKGLFILGLVIVSTVLIGKLYKSSIEKKTLEEFKEKFNY
                     SEEEKKKTLEEIKNGDGIALIDIEKISVHTVIAEGSTLDVLENNIGHFENTAMPGENG
                     NFSIAGHRNTINNEVFRNIDKLQVEDEIKITTLTDIFQYEINEIFVTSPSDTDVLNQN
                     LDEKTMTIVTCTNRGKDRYIVKAKLIG"
     gene            25154..26041
                     /gene="isdE"
                     /locus_tag="K8M77_000616"
     CDS             25154..26041
                     /gene="isdE"
                     /locus_tag="K8M77_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HBI7085133.1"
                     /translation="MFLKKGIALSLICLLGFGVMGCSNNSGESKESLKKENGEEIVVA
                     TSVAVTEILDELGVKVSGVPTSSYDLPESTKDAVKVGNPMNPDLEIIKSLNPDVVVSV
                     DTLGEDYKKLFTDNNIPSEFIDLTTLEGLKTSISTLGERFNKTEKANEILNELKVKED
                     EFVNLSKEEKKNVLLVFAAPGSMMIATPSSYIGNLVDKIGANNIVKDDKKPFVSYSNE
                     EIVKLNPDMVLVMTHGMPEQAKKMAEEKFASDPAWSRIEAVKEGKVYYLENGYFGMSA
                     NLKVTESLDKLGEIIYGEK"
     gene            26028..27014
                     /locus_tag="K8M77_000617"
     CDS             26028..27014
                     /locus_tag="K8M77_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI7085134.1"
                     /translation="MVKNNKLKSLYIGLSILIVLILMIISLKLGSIDISFGELIKGLL
                     SGSNEGNIGIIKDLRMPRVIIAALVGGNLALAGVLLQAVIRNPLADPQVTGISSGACL
                     VSLIFIIFMPASGSIRPIVGFFGGLVSCIVVYLMAYKKELDTMRIVLAGAAINALLGG
                     LSQILTIGSGIGAGNIQSWISGSLAATSWDEVKILFLYSIIGMGFALILAKSCNVIVL
                     GNKNAKSLGFNSDLQMILITVVAVFLGSVSTAIVGIISFVGLVVPHICRILIGSDHRY
                     LIPFSIFVGGALVLLADTIGRMLVKPYEIPAGIIMAVIGAPFFLYLLRRSDM"
     gene            27015..27794
                     /locus_tag="K8M77_000618"
     CDS             27015..27794
                     /locus_tag="K8M77_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085135.1"
                     /translation="MIRGEKLSFNYKGEKNIINDLNIDIKEGEITTIIGPNGSGKSTL
                     LSLLCALNKSKTGHVYLGDINMNNLKYKDIAKILATVHQQNSVPKDIKVEELVAYGRY
                     PHKGYFKNNNKEDKNIIKWALECTGLDDLKHKGVMNLSGGERQRAFISMALAQKPKIL
                     FLDEPTTYLDIFHQIEILEIVKKLNKDNGLTVVMVLHDINQAIKYSHNIVVMKDGKIV
                     KEGKSENVIDENLLKEVYKVNGVINKCKETGEIYFIPKTIC"
     gene            27836..29563
                     /locus_tag="K8M77_000619"
     CDS             27836..29563
                     /locus_tag="K8M77_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBI7085136.1"
                     /translation="MIDKRLLNLCKESKKYIGLTVLMNWISVICNLMTIIVIGFSIQE
                     LFSLGNIENLNKKILILAALLGVRFCVNLLSGHFSYKSSEMVKITLRDKIYEKLLKLG
                     MRYDKVDSTSSIVQMAVEGVEALEVYFSKYLPQLIYSILAPLTLFCALAFISFKAALV
                     FILCLPLIPISIILIMKIAKKILKEYWGQYSDLGNTFLENLQGLTTLKVFDVDNERHE
                     LMNEEAESFRKITMKVLSMQLNSINVMDLIAFGGAALGSIVALLQFRAGTVSLGGMIV
                     ILLISAEFFIPLRLLGSYFHIAMNGIAAADRIFKVLEADESKVSSDSNLNEISNISLC
                     NVSFSYDGKREVLKDISLSINKGDYVAIVGESGSGKSTIASLILNNYKASKGKITLNG
                     ADINNIDFSKLYNRVSIISTNSYIFNGTIKDNLLIAKKNATLEEINYALKKANLLDFV
                     NSKKEKLDLEVGEGGSKLSGGQKQRLALARTILANREVIIFDEATSNIDVESENMIWK
                     SINEISKEKTVIVISHRLANVKNADRIYMLDKGILVEEGSHKDLMERKNKYYGMVNYQ
                     NELEIGGVI"
     gene            29564..31252
                     /locus_tag="K8M77_000620"
     CDS             29564..31252
                     /locus_tag="K8M77_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085137.1"
                     /translation="MRKSGFSIMKRLIVELKPLAPVMFITVFMGVLGFLAAIGITVFG
                     SVAISSLIDSTISFSFKTALILMIVLGILRGPLRYGEQLSGHYIAFKILVILRDKVFL
                     ALRKLAPAKLEGMEKGNLISMITSDIELLEVFYAHTIAPISIAIITNTIMGLILLSIN
                     VKVGIVGIIGYILIGFGVPYISSSFGKEAGVQYREAFGKTNSYILDSLRGIKEVLIFN
                     NGNERRGNIKKFSEELNEKQKVIKKHEGIIRAFSDLIIMLTILIALYVAYLEVLKGTM
                     TIGELIIVVTLIASSFGPVVALSNLSNNLLLTFASAQRIFNILDEKPSVEEVLGEGMN
                     VGNNIEYKDVSFAYKGKENVLKDVNIDIKLGDKIAVIGESGTGKSTFVKLLMRFWDVT
                     KGEISLGNKNVKEIQTKELRKKMALMGQETSLFNMSIEDNIKIGKLDATLDEVKEAAK
                     KAGIHEFIIGLEDGYKTKVGELGGNLSSGEKQRIGLARVFLRESEIVILDEPTSNLDT
                     LNEGIILKSIRTYLKDKTLILITHRKSTTRVCEKVFRINKGNLIQCEEVKHELA"
     gene            31239..31634
                     /locus_tag="K8M77_000621"
     CDS             31239..31634
                     /locus_tag="K8M77_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HBI7085138.1"
                     /translation="MSLLKIFYVILGFISLGLGLIGIILPILPTTPFLLLTAFCFVKG
                     SDKFDKWFKGTKIYKNHLESFEKNRAMTLKTKLTILLTADIMLLFPLILSKSIHLKIF
                     IVILMMFKFYYFIFRIKTVKVGEVVEQNR"
     gene            31618..31914
                     /locus_tag="K8M77_000622"
     CDS             31618..31914
                     /locus_tag="K8M77_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBI7085139.1"
                     /translation="MNRIDKEKEIITLMIKLYCKKKHGSLNGELCNECKELDEYAHKR
                     LTYCKFGNEKSSCKKCPIHCYKKDMKEKVKEVMKFSGPRILIYNPKEYIRHIFK"
BASE COUNT        12876 a         3023 c         5360 g        10766 t
ORIGIN      
        1 aacctttaat aaatttaaag ttttttagaa taatatatta ttttataata ataaatcgaa
       61 attctaatga tgtttgtagt tttgtatata gtataaaatt atttagatct gttgtttttt
      121 aggattcaaa agttaatttt gaataattta aagcgaattt agaatgaatt ttttaaaaat
      181 ttaattttta gtttttttta ggtgtattac taataaagtc gataaattag aatttaagct
      241 tgataaatta gtacgtagac ttaaagaata tatttgactt ataatgaaga agagaatgtg
      301 catcctgtgc agcaaccatt gagaatatgt taaataaaat gggtggtgta gagaatgtaa
      361 gtttagattt ttaggttaca tccaactatg attctaatag ggttgaaata agagatatta
      421 gagaaagttt aataagttag aatataatat tataagtcag aaaataaatt ttaagataaa
      481 taattaagag gagttatatc aaaggtatat atttttgata taacttttct ttttacttga
      541 tatgtaaaat atttacaatt aaatccaaaa tgaaagaaaa tgagatttaa ttttatctaa
      601 tttaaataaa tgggctaaaa tacgatttta ttttttgtaa gaaaacatat atactaatta
      661 tatggtaaat atttacaaga tggagtcgaa aatataataa tatataagtg aagaaatatt
      721 aaagtggagt tgataattaa aattataagc tttatattaa ttaagtttga ttcatatgta
      781 aatactagag taaatataaa tgcaattaat ctttgcttta tattaattaa atttgattca
      841 tatgtgaaaa ataaaagatt tattaagtac cattagaatt agcactaaaa tattcattaa
      901 tattagtatg aatatagttg caaatagtaa aaatattttg atatatagaa tatgtcagtt
      961 tttataaaat tatagtttta ttaattaaat tgaaaatatg cataatatga ggaatatgct
     1021 aagtggttat taaagttagg gtaactattc ttaaaattta gggggattta aaatggagaa
     1081 tttattaaag gattattcca atgaagaggt tttaaaggca cttaagatag taactgagtt
     1141 gaaattagaa agagaacagg gagacttacc tgaatttatt aggggaaggg tttcagaggt
     1201 ttctgaagat gagtttgatt taatacaagc atataattat tatatgagag aattgagaag
     1261 gggatcagtt cttttaagtg aggaagagac agaaaagtgc ggatttaata gtaggttatc
     1321 catagatgtt gagttaaagg atttaataat ttttttaaat actcagccta atataaaaac
     1381 tttatattct tgttcagggc atcagtttgt aagaggtcaa ataacatttg aggggctttt
     1441 aaaatttcct caagtagatg attttaaaat tgaatatgca gatgaaaggg atgagacaaa
     1501 agtaagtttt gatataccgg aaagaagtta cgattattta aataagctta gaaagttata
     1561 caattatttt tatttaataa attataataa tttaaaaact ttaactaggg atgaggttag
     1621 ttcaataaag ataaaagaaa attgtactgc ataaaaaaat taccccgtta agtagatttt
     1681 aaacagtatt ttatactgtg tttacttagc gtggggtaat ttaatataat aaagctttta
     1741 aatttataaa aaataagttg tttcaaaaag gtattttcat aagtattttc ttaatgtact
     1801 tcataaatat cagaaagctc taactctata tttttatcat ctgttatata atcaactaaa
     1861 ttattgcttt cttctgtgta atttaatttt acatttggta gccatactat aaattctgaa
     1921 ccttttattc catcacttat aagttcaatt ttaccatcat gtagttcagc aatagacttt
     1981 acaatgctaa gacctattcc actaccttca tttcttcttt ttaaagattt atcagcttgt
     2041 acaaatctat caaaaattga tgcctgcatt tctttaggaa taccgattcc attatctttt
     2101 atgcttattt taacccaatc ctttgagatg tttaaatcca caaatatttt accatgattt
     2161 tgggtgaatt ttattgcatt tgaaagtaag ttaagaatta atctttcaat ttttacagga
     2221 tcacatttta ttatgttttc ttcaacatta gtatcaaaga ttatattaat atccttattt
     2281 tcagaatgag gaataaccaa agatactata ttttctacaa gaaatactat atctctatta
     2341 gtaaaatcag cttttaaaaa tccaacttcg attttactaa catcaataag gttatttata
     2401 agtctaagca ttctataaca atttattttt agaccttgtt tatatttgcc atagaaatca
     2461 ttaaaattca catagttagt ttcatgctta gaatttaata attgtattgt agagtagata
     2521 atattaagtg gtgttctaag ttcatgtgat atattggcga aaaattcatt tcttatttgt
     2581 tcataagcct tagcttcctt aaatttgata gctaaatttt caagacgttt tttagatgtt
     2641 aagttttttg caactataac ttttccataa ggaatttcat tattatcgct taaagttcta
     2701 attatagatt ctacataaac aaaagagtgt ttaccaacaa gtttacattt aatactatct
     2761 tcctcatctt ctagttttaa ttgatttaat atctcatcat ttttacctaa aaggtttatt
     2821 ataggcatac ctatgatctc ttcataggta taatttaatg tttctaatac agaatcatta
     2881 acatatgtaa ttaaaccatt attatcaaca cttattatta aatcctgtac gttattattt
     2941 atacaactta ataattgttc atttctttta atattttttg ttagggtata ttcttcagtt
     3001 atatctttta aattcacagc aaaataattt ttattttcaa catttaatat ctgtatgtag
     3061 gcacttacta ttttgtcgtt ttttaaaata atcttacctt cccaattgcc cttattaaaa
     3121 agtgatttag tagcattttt ttcagagtca tctataaaaa tcgaatctac aaataatttt
     3181 tctacctctt taagttgatc ttttaattca gttgatttgc aatagcgttc tcttattgtt
     3241 ttatttgtat atataacctt ctttttatta tcatatagaa ttatagaact actggtatta
     3301 aattcaacta aatgatagaa aacttggagt tcattttgta gtaaagaact ttttttgttt
     3361 ttagtcgtaa tttcattaaa aactccactt atagccaata aaaatgacag aatagtaagt
     3421 agaagaacaa cattattggt ttttaaaagt gttatatttg gaagaagtac ttcacaaatc
     3481 cagtataatc ctcttaatgc tattaaaaga atactagcaa ttataaatga atatattaac
     3541 tcgcaatcat ctaaagattt ctttgataaa aatatgcaag caattatata gaggataata
     3601 ataatagtca ttaaagtagc ataaaaataa atatctttag taaataactt attagaaaaa
     3661 attttatagt taatcatagg tgttataact gaaaataaaa atactgctac tgatgaaatt
     3721 gttttatgtt tagtaataag aggtgatatt cctttcttat taaaggcaac ataaaaaagc
     3781 aaaattatcc taaatattgc gtgtataaaa ataaacttat cgaatattat tagattatgt
     3841 cctttagtaa ttaaaagttc tacagataaa ttcatatata ttaaagagat tataaaaaaa
     3901 tcatcttttt tagtgcttga ataatatatt aatgaactta tgtatgccat tgttcctaat
     3961 attaatgata gacttaatgg gaaatttgga atagaattat taggaataaa ctctatattt
     4021 tttctaataa gaactttata agatagatct agtgttaata taaaaaatat agagaaaact
     4081 ataaaaatag atagttttat tattctttta gtttctaatt tatttttatc atcattaaaa
     4141 atatgaaaaa atattgaatt atccatgttt taaatctact actcaaaata ttgtaaaaca
     4201 tatacatatt gagaattttc cttcttcatt gagcctcgtc ttgcttaagc taatacgatt
     4261 ttttattgat ctcaaaaggc ttaaattccg aagttggcca tccgtacata tataagtgct
     4321 tttgatttta agctatctta tatttcttag catttttaag attaatactt gcgtttaaat
     4381 ctttatcaat agtaagtcca caatcacatt tataaatgtt aatcttttct gatgaagttt
     4441 tgtactttga ctatttgctt ttgaatattt tgactagtaa aatttcctcc tttttctttt
     4501 tatttattat tcttaaacct tcatatttcc ttgaaagttt tctttcctct ttttaatttc
     4561 ttttctactt ttttaacagt agatgctttt atttatattt gttatatttt atagcatata
     4621 ttagatttta acacatatat gcatatattc acaaaaaagt taaaaatata atattaaaaa
     4681 tacaatataa ttaaaaatag tgtaatgtta atttagattt aattataaat ttatattata
     4741 tttggaatta taattttaag aaatttttat atttatataa attatgggaa atataaatat
     4801 agattaatat atgcactata tcaataattt aaactaggag atacttatta attaataaaa
     4861 atataatcaa atatagtcta atatgatgta atatagtcac aaaaggatgt tattatatga
     4921 gtaaacatta taagtctaaa gaatttgcag aattattaaa tgtatcagtt attacattgc
     4981 aaaggtggga taattaagga aaattaaagg aatttagaac tcctacttgt aaattcaata
     5041 gtcaaaagtg gtacagtaag tcaaaaagct tatagatatt atgtatctat attggttgaa
     5101 gaaagagata taaaaacatc taatagtaat ataggtataa aaatatctaa ttcaaataca
     5161 ggtatgaaaa tatctaacac aaataataaa ggcttaggaa ttgatttagg tattaaggaa
     5221 tttgcaatat gttcaaatgg taataaattt aaaaatataa aagaagggag agctactcgt
     5281 caaaatatac aaaaataagt agtcaaagta caaaaacttc atcagaaatt agctaatata
     5341 cgaactgatt atataaataa aacagtattt tcaattataa agcaaaaacc aagctatata
     5401 acgattgaag atttagcagt atctaatctg atgaagaata agcatttatc taaagctatt
     5461 gcaagtcaga agttctttaa atttcaaact aagttaacag ttaaatgtaa agaaaataat
     5521 atagaactta gaatagttga tagattttat caatcatcaa agacttatag tcaatgtgga
     5581 aaggttaaaa atgatttaaa actatatgat agagtttata aatgcgattg tggatttact
     5641 attgatagag atttaaacgc aagtattaat cttaaaaatg ctaagaaata taagatagcc
     5701 taaaaacaaa ggaacttata tatgtacgga gggctaactt cggaatttaa gcctttggag
     5761 agccatacaa aatcgtagta gcttaggcaa ggcgaggttc tatgaagaag gaataatctc
     5821 catatggata tatttgagca tattttgagt agcagaaata atatgaatgt tgttttaaaa
     5881 caaaggaaaa gattagatta tttagacatt tcaaaggcat ttcttataat aagtgttgta
     5941 ctgtgtcatt cgccttttga aaatgcgtat tatctttatt ggtttcacat gccagccttt
     6001 tttataataa gtggaatgtt atataaaaga ggaatgaata taaaggagca agcaataaaa
     6061 ttttttgttc cttatgctat attttcatta atagatatta attttaatta tattatgtat
     6121 tatcacggga atttttcttt aagtgattta attcatgaaa ttataaaata ttcatatggt
     6181 ggaaaggcca catggggagt gttttggttt atacctgtaa tgtttgttac aaaggtagta
     6241 tttaatcaat taaataagag attaacatct aagaagttag cttttttatg tatattaagt
     6301 tatatattag ctcacatata ttctatgagt tttataccat caagtgtaac tgagataact
     6361 gaaaatcaat ttgtattttg gaacttagat gctgttttaa taacattacc atactatgca
     6421 ttaggatact atataactaa attaaatata caaagagtgt ttaaagaaac ttctacatta
     6481 attttatcaa gtataggggt agtagctatg tttatattaa atagtagtat gggaatagga
     6541 tattacttaa atataaaata ttcttactac aaagatccta tttttgatat attaatgcca
     6601 gttaccataa caatttttat actttcctta agctatcaaa ttagtaagtt taagtgtaag
     6661 aaattattta ctataattgg agctaattct cttgtaataa tgtatttaca taaacagatt
     6721 gcaactttat ttatggatgt atttaattat ggatatataa tgtttactat aattggagta
     6781 tgtttaccac ttcttatgat ggtattaata aataaaagta ttgtcagcaa atttttattt
     6841 aatggagatc ttactttcta tagaaagatt aaagatatta aggatataga accaaaggat
     6901 ataatcatag ataataaata gttattaggc ttagaaaagt ttttctaagc cttttttgta
     6961 atatttaacc ttaaagaata atataatcat aaatttttat attttaaatg ttttaaccta
     7021 aaaattaaag gtatataata taataagaaa ttcataaaat gtttataaaa taactgtgaa
     7081 tggaagggag tagcgatgtt gcaatgacaa tgcagaggaa aaattttaat aattataaag
     7141 aagtatccct attaataatg ttaattccaa tgttatttat tttaatagat accttaggga
     7201 gaatgaattc taaaataaat aataaaaatt tgtttgaaat tatatttaat gagatatttt
     7261 tatacagaga agttatagtt aagtttataa tggccatagt tgtcatattt gttatatcaa
     7321 agataaaaat tataaaagag aaattcatat taggtgattt taaaattata ataggatata
     7381 ccacgctagg aataataagt tataaatttt ttggtagtga atacgggctt tctaaatctt
     7441 tagatgtata tgttatgtgt ataatagcta tgatttttat attatcagga ataagagaaa
     7501 ttatatcatc tatagataat ataaatatta atattgaaga gaaaagtaat tttgaaagat
     7561 tagtatatac aagagttaag tatttaggaa taatttattt tataatattt tcaatattaa
     7621 tagctataga aaatttaaat agtataatgt ctagtgattt tatagtaagt ttaattagca
     7681 attataatca gcattataat aatatggcca gagtgatttc ggtattagga gtggttttaa
     7741 tagcagctct aataattata attaaaaaat cattttctaa cttaaattta tgctatgaag
     7801 aatatattta cataataaaa cataaaactg ttactgaaga gattaaaaaa gatatagatt
     7861 ttgttaatct taaagttgag gctaataaaa aatacattaa taataaattt aaaaagcttg
     7921 aagacaaaaa aactcaagta ttattaaaag gtatttttga tttctttaag aataacaagc
     7981 attaaataaa tacatattct tagaggggaa agtatggata gtaaaaaaag tacaaattat
     8041 gattttatat taaaaacaag tattgaaaga tattatgatt tattgaaaaa tgctgaaaaa
     8101 gcatgggaga cccatgcagc tttaacatat atcgctttaa gaatggttgt ggaagactta
     8161 ttaattaagc ttgaagaaaa atatgaagta aatagagata aagaagataa ttttaaaaat
     8221 accttaaata agttaaaaga atatgtccca agttggataa ttgagtatgg tgagtatata
     8281 agaattaaag gggagaaaag tgctaataaa gaaattgata gcaatttaga agaaaatagt
     8341 acattattag cattaaagaa agtgcatgaa attttaatat ggtacttaaa aaatgtaact
     8401 aaagagatga gcttagaggc ggattatagt ttcattactc cggaaagtat aaaagagagt
     8461 gaatgtgact tagcaataga taaaaaggtc cttgaaaata aggaaaaatt tattataagt
     8521 ctagaaaaac aaataaaaac taatagtgat gatgaaaaaa aacaagaaga gcttgaagaa
     8581 cttttaagaa atgttaagga agaaaagaag gagcttacag aaaaactaga gaagaaagag
     8641 ttagagcttc aggaagtttt aaatagtttt aaagaacttc aagttaaact tactacttat
     8701 atagagaagg atcaatctgc tgaagaaagt aataataaat atccagattt ttataagggg
     8761 tttttatcct tacaaggata tgaacttagg gttgtctatg cctttttgaa gagtttaagt
     8821 ttaggtagta ttttcttaaa taaatctaaa agtatatttt taaaatctca aaggaaagag
     8881 tttttaaagt ttattgaaaa agaggtagag gacttaaaaa aatattctga cgggatttta
     8941 aaattaaagt tatattatat tttattaaag cttactaata ctccagtagc ctcagttgca
     9001 gataaggaaa cttttgataa atctttagat agtatagttg agaaggctta ttttataatt
     9061 gaaagtcgta atggatttaa tggtaaggta gataaattag aagcgataat aaattactat
     9121 ttaatagtaa taataaatag cttgtcagct gaaataaatc atgaagagga taatgaaggc
     9181 aaggtttctg atataattta tgaagtttta aaaacaatac ctttagatga gagaaaaatc
     9241 atattaaata agatgaattt tacagatata tctgaggggg caattaagga aaatataaga
     9301 ctaaatcaag tggagttttt aaaagctgcc ttagactgtg gaataagtga gagctctgaa
     9361 tttgttataa aatcagtttt ttcaatttat gtagcctttc aaaaagattt tgatttttat
     9421 gaatttagaa ctagacatga tatgttaaga gcttatatag gtcctttatt actagtaatt
     9481 cttttaaagg agtttaaaat ttcaagagag aatttagttc ctatttttgt aatggaaata
     9541 agcatatcaa ataaaatatt aagaaaagaa agaagagaaa aaatatacta taatcagtta
     9601 gttcaggctt ggttagaaga aaaagagaaa tatgataaaa tactttcaga aaaagataac
     9661 ttagaaaaaa atcttaagga agtgcaagag gcaagggaat tattattaac aaatgagaga
     9721 aaatttgtag ctgaaaaaga taatactgaa aatgctataa aatctcaatg ggatatgttt
     9781 aaagaaaagg ctcttcaatc tgaagagatt aaatccttta attcttatga agaatataga
     9841 agggctgtta atcaaaaaaa tctcatagag gtgtcagaag aatatagtct taaggatagg
     9901 gggattatta aaaacttctt ttcattagat acatggaagt acaagctaaa taaattagaa
     9961 ataagtaaaa atataaaagt tttagaagaa aagcttattg aagaggctaa ggaatctgga
    10021 gtttttgaaa atgagctttt aaccataaga gaattagaaa aaatattaga agataaagag
    10081 agtggcttag aagatataag agttgattta aaagaaaatt cagttgaaaa tgaaaaatta
    10141 aatagactaa taagtaatag cagtaaactt atggaaaaat ttaaagaaga taactttgat
    10201 tttgctaaat aaaaaagctg gtatttcata ccggctttaa atttatatta aaaatattta
    10261 aaataacttt tgtcaatatt ttgaaaaaag ttattgacaa aagtcgttga aaacgttatt
    10321 ataaaattaa gataattatt taagatttag tgtggtagga ttgataaaag cctaaattaa
    10381 atgtgtttat ttgctcattt aatttaggct ttttattccg ttacatttta tttagttaag
    10441 tttacatcca tttgaggata tggaatagtt atgttttcct catcaaatct taatttaact
    10501 tcctctagta agctaaaatg aaccttccaa taatcttctt gtttagtcca aactctaaca
    10561 gtgaaatcta tagaacttgc agcatgttgg cttataccta caaatgcttc aggtgttttt
    10621 aaaatcaact catttttatt tataatatcc caaagaacat ttttaacttt gattatgtca
    10681 tcatcatatc caacgctaaa tattaaatca actcttctag tatcttttga tgaatagttt
    10741 ataagacttc cattagctag tgtaccattt ggaataagaa tttctttatt atctggtgtt
    10801 actagatgag tatagaacac agttattctt tcaacagttc cagaatatcc tgaggcttct
    10861 ataaaatcat taactctaaa aggtcttaat aaaagaatta ttactccacc agcaaaatta
    10921 gataaacttc cttgtaaggc aagtccaact gccaaacctg ctgaagctat aagagctgct
    10981 agtccagcta tatcaaatcc aacataactc attagggtta taactagaag aacttttaga
    11041 gatacctctg aaaaagcttc tgcaaatgat gttaatgtag gatctacatt tctacttttc
    11101 attatattag ctaaaatctt aattacttta ttgataattt tccatccaat ataaagtaga
    11161 atcaaaccaa ttactaattt aaagccatta gttgtaatcc aagataaaag cttttcgtac
    11221 atcttactaa gatcgaactt cataaataaa cctcctatat ttattgctta ttaccaaagc
    11281 aaatatatta taaattttat tttaacaaat atcaactaaa aagaagcaag ataaatagat
    11341 aattgcaaat gatttagtgg gatatatcat gataaaaaat aaagtgtttt caaattagag
    11401 aagttggaga agttttaact gttagctgaa tttttgaaaa tattaaaaat aataattaat
    11461 ttatgtttat aaatcttaaa attagggaag tatataagat gaaaagacct tataaaaacc
    11521 atagttttgg gcagaactat ggggtggcat tactagggaa agagctatgg agtatccata
    11581 gctctaattt aatataatat ttatattaaa agaaatatga aattaaagga taaagtcata
    11641 taaaaaaggg cttataccaa agtaacttct taacaaaact tctaaaatgt atcttaagaa
    11701 attatgagat tcaagccatt ctatagatta tataacattg ggagtaagca ctataaattt
    11761 tcaatttaat aattctcaag atgtttataa gttttatatt taagtttttg atataatccc
    11821 tttttctaat atatttttag gagtataaat tatctattaa atcttataaa gtctaattca
    11881 tttgaaattg ctatgttata aacataagaa ttagcaactt catttatgaa tctttctttc
    11941 atttcttcgt ttaattcaat attgttaagt ttattatgat agttcataac gaaacctttc
    12001 atttcaggtc catcacttaa agtttcatat ttatagtaat ttaatccttc ttcatctatt
    12061 ttatataaat cttttaccat accatagatt gttcttccac caaataaatc tgcaagatat
    12121 ctagtataag catgtgctac taaaagttca ggagcagttt ctcctatttc atttattcta
    12181 gcaacataag ctcttgtaga agctaatggt ttcattgtat ttaaattttc tcctaataag
    12241 aattttaaat ctttaagtat agcttcagca cgataaattt caggaagtac aaagtcttta
    12301 actactttgt tatctttgca tttttctaag tttacttcta ttgcgttata aacttcgtaa
    12361 agattataaa gatattcagc gtagctttca gtagaagctt ttccttctaa taatcttttt
    12421 aagaatcctg ttttttcagc tacagcatgt aaatcgttac tattgttttt tatatccatc
    12481 ataaatgaat tcattgaaat cacctcataa atctattcaa aattttaata tgttaaagtt
    12541 ttaatatctc ttttataaat tcaatatact atttaaaaat atcataatca aatattagat
    12601 tttatgaatt agtttcatca aataattatt ttttatacaa tttaataata aaatatatac
    12661 ttttttaaaa gttaagccaa caattatttg agaataatta ttattaataa aaaatactag
    12721 aattttatta ataatataaa tttaaaatag caatttattc tataaaatat ttatcaaaat
    12781 cttattaaat ataaattggg tttagtataa tgtttataag gattaaagtt agaaataatc
    12841 ttatgtaaag aggaaagttt ctataaattt aaaaaggtgt taggagagat gcggaatgag
    12901 gatacttcac actgctgatt ggcatttagg aaagaactta gaaggattaa gtcgtatgga
    12961 tgagcaagaa cgatttttag aggattttgt tcacatagta aaagataata atatagattt
    13021 agttattata gcaggggatg tatatgattc tagtaatcct ccagctaggg cagaaaaaat
    13081 gttttatgat accttaaaaa agatatcagc taatggagag aggatgactt tagttatttc
    13141 aggaaaccat gataatccag aaagattagt ggctgcaggg ccattagcaa gagaccatgg
    13201 aataatcatg gttggaactc ctaagagcat agttgaagta ggggaatatg gaagaaataa
    13261 agttttaaat tcaggagaag gatttattga attagatata aatggagaaa agtctgtaat
    13321 attaactgtt ccatatccaa gtgaaaagag attaaatgaa gttttatatg gtgcaatgga
    13381 tgaaacttgt gagaggataa aaagttatag tgatagaata aagcttttat ttgattccct
    13441 taaaggaaat tttaaagaag atacaattaa tttagtggta tctcatcttt ttgcaacggg
    13501 agcagaaaat actggttcag agagaaatat agagcttgga ggaagtttta ttgttgataa
    13561 ggaatgtttc ccagaagaag cccaatatat tgccttaggg catattcata agcctatgat
    13621 tctaccagga agtaataaaa agataagata tgcaggttca ccacttcaat ataataaaaa
    13681 agaaataagc tttgaaaaaa agtgtttcat tgtagatgtt aaagcaaatg aggaggcaaa
    13741 tatagatgaa ataaaatttg atgtgtacaa gcctatagaa gtttggaagt gtgatagcat
    13801 agatgaagct atttacaaat gtgaagagaa taaagataga gaatgttggg tttatttaga
    13861 gataaaaaca gatagatata taagagaaga tgaaataaag gctatgaaga agcttaaagg
    13921 ggatattatt gaaattattc ctaagataac actagatgaa gaggagaatg atgaaagaag
    13981 tttttcagaa aaatcttttg aagaattatt cactgacttt tacgaaaagg aaagaggggt
    14041 taagccagag gaagatttat tagagctttt acttaatata gttaaagagg aaggggatga
    14101 agaaaatgaa accaattaag ctagaggtta agggattaaa tagttttata gataagcaag
    14161 ttgttgaatt tgataagtta acagagagag ggctttttgg tatttttgga cctactggaa
    14221 gtggaaaatc tacaattcta gatggaataa cccttgcttt atatggtgat attgcaagaa
    14281 aaagctcaaa ctatataaat actaactgtg atggcgtcta tgtaagttat gagtttcaaa
    14341 taacaggtaa tgaagtaaaa aggtatagag ttgataggga atttaaaaga gataataaaa
    14401 gtggcggaat aagaaataaa agttcaaaga taattgatat aacaggcgga gttgaaaatg
    14461 tactagagga aaaggcaaag gctgtaactt caaagtgtga ggaaattata gggttaaaat
    14521 tagatgattt tatgaggact gttgttctgc cacaagggaa atttagtgag tttcttaagc
    14581 tagaaggaaa agagagaaga aatatgcttg agagattatt taatcttaga aaatatgggg
    14641 atgatctttc tagtaagctt agtttggaaa taagaaaaga aaaagataag atgaatgtct
    14701 tagaaggaca attaaagggg tatgaaggag ttagtgaaga agctttaaaa gcaaaggaag
    14761 aagaaattaa tgaaatcaac ttaagtatta aaagtaaaga agagttatta aataagataa
    14821 aaaaagaatt tgaggaagcg gaaaaagttt ggaatactca aaaagaactt tatgacaaaa
    14881 gaattgaaga agagagttta gtaagtagaa gtgaggaaat aaaatctttt aaagaaagag
    14941 ttgaaatttc aaataaggca gataaggtaa tagtatttat aaataatctt gaagaaattt
    15001 taaaggaaat aaataaagag gatttaaaat ttaatgagtt aaataagaaa ttagaggaac
    15061 ttacaaattt aagagaagaa aataagctaa agtttgaaga ggtttctaaa aagaaggaag
    15121 aaaaattacc tgacttaaga ttaaagaagg aaaaattatt agaaagccaa aaggaaaggg
    15181 atatactttt tcaaataaag gctgatgggg ttaaattaaa ggaagcttgt aagaagattt
    15241 ttgaggatag aagtaaatgt gatactaaat taaattctat agaagaaaat gaaaagagat
    15301 taaatgaaga attaaaagag aaggaagaga gaaaagaaga gctttttgta catgaagaat
    15361 ttaaaaataa gattaatagt ggattattta ttttaagttc ttatgaaggc ttagataaac
    15421 aatttaatga gataaagagt gaagaaattg aattgaagaa atatataaaa aatcttacag
    15481 aggataaaga aaagtcagag aaggatttaa aagtaaagat agaaagttta agtaagacaa
    15541 gggacaaact agaaagtctt ttaaagaaaa caccagggga tagtaattct attttagaaa
    15601 aacaaataaa acttggagag tatagagaga agcttaataa atataaagag ataaaaaatt
    15661 ctttagaaga gtccttaaag agtaaaaata attttgaaga aaaacttaaa gcttttgaaa
    15721 atcaaaaatt attattagaa aaagaggtaa gagagcttaa ggagtatata aataaggtta
    15781 aggttgaaga gttagctcat aaacttaggg agaacttagt agacggagaa tgttgtccag
    15841 tttgtggatc aactcaccat gaacttaata aggttgagaa aattaattta gaggaatcaa
    15901 atgaaaaaac aactttatta gaaagtaagg aagaaaagtt aaaagaactt attttagagt
    15961 tctcaaaaat agaagctacc ttagaatatg aaaataagaa aattgaggaa ttaaatattt
    16021 ccatagaaga agtaggagaa gttaatgagg aaagattaaa atccttagaa gaagagttta
    16081 atactttaaa agagaaggta gaggagttta cccttaaaaa agaaaatcta gaaaaagatt
    16141 tagaaaaatt aaaggaagaa aagaataatt tagaaaatat cttcaataag gctgaggtta
    16201 ttttatgtga aaaaatagtt agagagaaag aaatagcttc aaagataaag gaagtagata
    16261 aggagttaaa gttaaaaaat tctgagctta attccataaa aaatgaacta aagattgagg
    16321 atattaagct agagaatgag ttgatattaa aaaaagagaa agaaaaaaat ctattagaga
    16381 aagagattag aatattaaga actcagctag aagaatcaaa taagataaag gatgaattaa
    16441 gagaaaaaag agatgcatta aaggaggatt atttaagtca aaaatcctta ctagatggta
    16501 aagtagaagt ttatagagaa aaggaaagaa tgattaaagg atctttaaag ggcttaattg
    16561 atgaagcttt acctattgaa aaaatagata taaaaggctt attagaagat ttacaattag
    16621 agattgatta tatagaaaag gcttatttaa atctaagtga ggaaaaagaa aaactagaaa
    16681 aagcctttaa taatatgaat caagaggtag ctgttactaa ggaaagagtt aattcattaa
    16741 atcttagaaa agaaaatgag gagaaaaaag taaacttagc tttagaggaa gaaggattta
    16801 agacaatatt agaggttaaa gaaggtattt tatctaagga tgaaaaggaa aaattaaaaa
    16861 cattaattga agaatatgat aataacttga ttaaggttag agcaaatata gaattattaa
    16921 ttaaaaagtt aaatggaaag agtttaacag aagaagaatg gacaagggtt cttcaagaga
    16981 aaaataatac tgaaaaagaa ttaaaggaag ttgaagaatt aaaaattaga cttgttactg
    17041 aaaatgagtc tataaagaaa aaacttgaag aacaaaggga tatacttcat attaaagcta
    17101 aacaagaaca taagcttgct cttttaagtg atttagaaaa attatttaaa ggaaagaagt
    17161 ttgtagaatt tattgcagca aatcaattaa aatatatatc catagaggcc tctaaaaagc
    17221 ttaaggatat aaccaatgga gtttatggat tagaggttga tgaaaatggt aagtttataa
    17281 taagagatta taaaaatgga ggtgctaaga gagatgcctc aactctttca gggggagaaa
    17341 ctttcctagc ttccttagcc ttagcattat cactttcatc acaaattcag ttaaaaggaa
    17401 cagccccttt agaattattc ttcttagatg agggctttgg aactttagat gataatcttt
    17461 tagatgttgt tatgagttcc ttagaaagac ttcatcacga aagactatct gttggtataa
    17521 taagccacgt tgaatctata aaaaacagag ttccagttaa gcttatatta acaccggcag
    17581 aggcaggaat aggtggaagc aaggttaaaa tagagagaag ttaaatcaaa atcaaaagag
    17641 cttaataaat aagctctttt catttaatat ttaaaaaggc tttaaattta gttaaagaga
    17701 tttatatatt tattattagt tttagataat agatattcaa aataaatctt gtaaaattat
    17761 tgagatagtt ataaatagga gtgtttatag aaaatagaaa tttttttatt ataaataaag
    17821 tggttataaa ataatttgtt acataaatta cctaatttgt agttattata ttattttctt
    17881 taaaataaaa taattttacg aaaatattta caaaatcttt ttatgtttaa ggttgaaata
    17941 tattagatta gaatataaaa tttatcttaa gattggcatg ggttaaaggt ttaaatcaat
    18001 ttaactgaga ttaggagagg gataaaaatg aaaatactta taattaattg tggaagttca
    18061 tcattaaaat acaaattaat tgatatggtt aatgaaaagg atatcataga ggggataatt
    18121 gaaagaatag gacttgatca atctagatta gtccaaaaaa atgaattgag agaaaaatat
    18181 atattagaaa aagaaataaa agatcataaa gaagctatag atatagttct aaacacttta
    18241 gtagattcaa aggttggagt tattgaatct attgatgaaa ttacagcggt aggtcataga
    18301 gtagttcatg gaggagaaag atactcttct tcaataatca taaatgaaga ggttataaaa
    18361 tatttagaag agtgcagtaa attagctcca ctacataatc cagcaaatat aattggaata
    18421 agagcatgtc aaagtcttat gccaaataaa gaaatagtag ctgtattcga tacagccttt
    18481 catggaacat taccagaaaa agcttatata tatgcaatag attatgcttt atataaagat
    18541 cataaaatta gaaaatatgg attccatgga acttcacata aatatgtatc tcataaggtt
    18601 gcagaaacta tgggaaaaga tataaaagat ttaaaaataa ttacatgcca cctaggaaat
    18661 ggtgcaagta tatcagcagt aaaaggtggg gagtgtatag atacaactat gggatttact
    18721 ccacttgctg ggattccaat gggaactaga tcaggtaata tagacccatc aataattcct
    18781 tttttagttc aggaatgtgg atatactata gaagaggttt ctgaatcctt aaataaaaaa
    18841 tcaggagttt taggaatatc aggagttagt tcagatttta gagatataga agatgccgct
    18901 tcaaatgggg acaagcgtgc acaattagcg ttagacatat tccactatag aataagagct
    18961 gaaataggtt catttatagt aaatatgggt ggtgtagatg taatagtatt tactgctggt
    19021 gtaggtgaaa attcacctga aactagagaa gaatgtttaa aagatttaga attcttagga
    19081 ttaactttag ataaagaaaa aaataaagtt agaggaaaac ttgcagaaat atcacaagct
    19141 gattcaaaaa ttaaagcata tgttgttcca acaaatgaag aattaatgat agctaaggaa
    19201 actgtagaat taataggtaa ataatttgtt tagaaaaagt attattgata tttatttaag
    19261 aaagaatatt ataaattaag tttaaagtaa tattaaaact aaaaattata ataaataaaa
    19321 gttgagaatg tagggatttt atttgaaaat aaatttctta cattctcatt tttttataag
    19381 atatagttac gtattcaatt atgaaaaata atttttatag attactataa tatttttcta
    19441 ttttaatatc tacttcataa tctattattt aataagtgaa caagtgtgtt ttcttgtaat
    19501 cctaaattcc tagagtttaa aagttaagtg ctaaattaaa attattttaa agtaaaatat
    19561 agtaaatttt gaaaattata ttcttttatt gtataatatt tttatgaaat ttatgttaat
    19621 tttgtttaaa atttatttta tatacagata ggagttttta tggggtttat tgtattttgt
    19681 ttgatagcat actttgtaat atctaatttg tcaaataaga aagatgttag tggaaataaa
    19741 gctaatagca attgtccata ttgtaatgca gctttttact taactgaaaa tggagatttt
    19801 aattgttcaa attgtggtcg tttattcaaa tatagagatg gtattgtgta taggcaggat
    19861 gaaagattac ctattgtagt tgagttagca tgtacacttt ttacaatatt atgtaaagct
    19921 gatgggattg taacaaaaga tgaagtttca ataactaaag aactacttga aaaaaatttt
    19981 ggattagaag ataaagatat gaaaatggct atagatattt taaataagtc taaaagtaag
    20041 ccatataata aaaccataat aaatgattta aataatattt ttaataatta tgatttttct
    20101 aagatagatg ccgaagacta caaagaactt atacttagat gtgctatgat tatagcattt
    20161 tgtgatgatg gagaaccttc taataatcaa aataaaatat tagatgatat tgttagtata
    20221 tttaatatat ctgctactaa atatacaaat ttattaaatt attttagaga taactgtatt
    20281 gaaaaaaata aaaataatta ttatgaaata ttgggggttt cagaaggagc tagtaaagaa
    20341 gaaattagag ctgcttttag aaaattaagt aaattatatc accctgatag atattcatct
    20401 aaagatcttc ctcctgaaat aataaaagaa tttgaggaga agttagcaaa aataattgaa
    20461 gcttatgaag cattaaaata aacgaaatta attttaaaaa tatataataa aaacaataac
    20521 tttaaattag agatattagg tagcttgaaa ttattttaat tagatattac tggaaataat
    20581 gatttccagt aatatctttt tagaattagt agtaagtatt gttataaata taataagtga
    20641 agtttaccat tatagtttta gtctatttat aaaagttata atatctttag taattacttt
    20701 acacatctat tttctaaaaa tatttaagtt tacaaacctt ttttatagtt atcctctctg
    20761 tattttaaag gagttagacc agtaaatttt ttaaatactg tgcagtagta actttgatta
    20821 ctaaatccaa ccttatgtgc aatatctaaa agtgataaat caagatcctt aagtaagtcc
    20881 ttactctttt ttattctgta attatttaaa aagtttataa atgtttggcc tgtttcattt
    20941 ttaaatatat tacagaagta ggacttattt attttaaatt ctgtgcaaag agagtctata
    21001 gtaatttctt ctgcgtaatt cttttctata aaatctatag ctcttcctat ataaggagaa
    21061 tatgaaattg aaaaattatc tttaagtttt tcatctaaga ttattttcaa tagagaatta
    21121 taatacttaa gacagctttt agtctttaaa ggtattttat aaagttctaa atcatcatta
    21181 gaatttttta aatcttcttt taaatatagg ggacctatta tgaaaattaa gtctttatca
    21241 attgatttat aattaaacaa tgaataaaaa gatatattat tttcaaaggt aattattagt
    21301 gagtcattat gatcatgttt taaatatgag cgctcaaact tgctatttaa gtttaagtta
    21361 ttaaatatat aatcataaaa agaagagtaa ccaacctcag aaagaatatt catattatta
    21421 tctacagctc ttattggaat ttcacagcag ttaaaaaaat ctacaagaat tgattttaaa
    21481 aaaatcacac aaaatcctcc tttttaaatt taatatatta aaaaaaatct taatatagag
    21541 aaaaacaatt ataaaaatct atgataatat aagtatatta acattattga taatcatttt
    21601 caatgggaaa agaaagtcaa agggggatta aatttgggaa gaaagaaaat taataaaatt
    21661 ttaggttatg cacttgtaac atctatgcta gttggagtag gagcagatac tgtatatgca
    21721 gccactaata ttaatagtgg aagtgcaatt gtgcgttcag aaggacttag agatggaata
    21781 tatgaagcta ataatgtaac ttcatatgtt gaagaaggaa attctacagg ggaaaatatg
    21841 gcaagaaatg ctataaaaga aaaaacaaag tttaagattg aaaatggaaa aacattaatg
    21901 actgtttatt ttaatagtag tttatatgga tttatgaaca atattgaagt tagagctgga
    21961 ggagaagatt taaagataga agaaaataaa gatgataaat caataacatt tgaagtacca
    22021 tcacctgata caaaggtaaa aataggatta ttcataacaa tgatgggaag aaaggttgag
    22081 cttttcttag ttaatgatat gaatactgtt aatttattag atgaagcacc tactataaat
    22141 aatgctaagg atattagtgt tactcaagga gatgctattg atttattatc tggagttatt
    22201 ggaactgata aggaagattc taatttaaaa gttgagataa gtggagatac atcatttata
    22261 aaagatggga aggcagagat tcctggagta tatcctataa cctataaagt tacagattca
    22321 tcagggcaat ttgatgaaaa aacagtaaat gttacagtta ataaaaaaac aactttaggg
    22381 gatggaagtt atatccttaa gaatacagtt caatatgttg gacaaggaaa catggaaaca
    22441 ggaaattcca tggctagaaa agtgttatca gagaattcaa gaattgatat atcaaatgga
    22501 aaaaatacag tcacattaac ttttaatagt gaattatatg ctttcttaaa gaacttcaat
    22561 gtaacagttg atggagaaaa agtagaagca gaagtaaata aagataatag aactataaag
    22621 tttaatatac ctgatttaaa ttcagacatt gttgtatcta cactagttag tatgatgggg
    22681 aaagaagtta gttttaaaac tactttaaat tatgatacag ctaagaaatt agaaaataat
    22741 ttagaagata ataataagcc tggtaatgaa gatagtgaga atattccagg tggaaatgag
    22801 aatagtggaa atcaagataa ttcaggaaat actaaccaag gaagcaatgg aagtgcaaat
    22861 gaagataaag aaaatgaaag tgaaggaagt tcaggagaaa tagtagatgc aaatcaatta
    22921 aagaatggta tctataacat aaaaaatgat gtttcataca ttggtgatgg aaatcaagat
    22981 gtgggaaatg atatggcaag aaaagctcta tctaagaata gtaaattaga ggtaaaagat
    23041 gataaaaaaa ttcttacact aaagtttaat gaagagcagt tctcattctt taaggacttt
    23101 agaataactg caaatggcaa agatattata gcaacgccaa atgaggctga tagaacaata
    23161 tcttttgaga taccaagtct tgatgctgat atagttgtta cagcctttgt aagtgtaatg
    23221 ggtagagatg taagctttaa aacaatactt aataagggaa ctttagaatt agttagtgga
    23281 gaggacaaac ctgccataga agaagagaat aaatcagaag aaagtaacgg tacaagctct
    23341 tctaataatg gaagctcagc taacgaagga aacagtacag ttacagaaaa taaagttact
    23401 aagggaaaac tttacacaat agaaaacaaa gttgttcata aaagtcagac tggtgttgat
    23461 atggcaagaa aatatttaaa caaaatatct gacttagaag aaattgatgg aaaaacttat
    23521 ttaacattaa catttactgg tcaagagttt atgaaagacc acaagatatc tgtaaatggt
    23581 aaagatgcca actataaagt tgtatctaag aatggtgatt caattaaatt aagatttgaa
    23641 attcctaatt tagatgcaga tattgaagta agtttatatg taataccaat gggaagaaat
    23701 gttgagttta atgtagaact tttaaaagat actaaaaagt ttgtaaaaga ctttacccta
    23761 tcaagcttac cacagactgg ttcaccaata ggaggaaact cagttgcatt attaggaatg
    23821 gcaatgatgg gagcttctat gtttattaga aaaagagaag agtaaaagca gtttagtttg
    23881 agaggatttc ctctcaaact atttttaaac aaatggaggg aaattagatg agatttttaa
    23941 aacctgtttt attagttatg ttaacacttt ttatttcttt aacaggagtt tttggaagtg
    24001 ttgatgtact agcaaatgaa aacttagaaa atggaatata tgatgttaaa aatgatgttt
    24061 atcatgattc agatataggg atgtcaatgg ctagaagcta tttaaatgac actatgcagc
    24121 ttaaaatttt aggagatgaa aaagaatata ctataggttt ttcaggaaca gaatatatgg
    24181 aaaatttcag aatcttagta gatggtaatg atggaaatgc tgagattgtt gaggaagata
    24241 aagagaatgg aactataaaa ctaaaagtta aggttcctaa taaggattca gagctagagg
    24301 cgaaaatata tgtaggacct atggaaagag atgttcaatt taaggtgatt ccaaagtttg
    24361 atactttaac tcttgttgag aagttagaag aacctaaagt ggaaaacaag gaaagtagta
    24421 gcgaaaatct ttctgatgaa actggaaaaa gtgaagatgc tataagcgaa gcaacaggtt
    24481 tagcaaataa taagaatatg ttcatttata ttggtggcgg aattataata gttgctttaa
    24541 tagtttcttt agtaatattt aaaaggaaaa agtaacatga gaaataaact agctaaagga
    24601 ttatttattt taggtttagt tatagtttct acagttctta taggaaaact ttataaaagt
    24661 agtattgaga aaaaaacttt agaagagttt aaagaaaagt ttaattattc agaagaagag
    24721 aagaaaaaga ctttagaaga gataaaaaat ggagatggaa tagctttaat agatatagaa
    24781 aaaataagtg tacatacagt tattgctgaa ggtagtacct tagatgtgtt agaaaataac
    24841 attggtcact ttgaaaatac tgctatgcca ggcgaaaatg gaaattttag tattgcagga
    24901 catagaaata ctataaataa tgaggtcttt agaaatatag acaagcttca agtagaagat
    24961 gaaataaaaa tcacaacttt aactgacata tttcagtatg aaattaatga gatttttgtc
    25021 acctcaccta gtgatactga tgttttgaat caaaatttag atgagaaaac aatgaccata
    25081 gttacatgta caaatagagg aaaagacaga tacatagtta aagcaaaatt aataggttag
    25141 tggagggaaa atagtgtttt taaaaaaggg tatagcttta agtttaatat gccttttagg
    25201 ctttggagtt atgggatgtt caaataactc tggagaatca aaggaaagtt taaaaaaaga
    25261 aaatggagaa gagattgttg tggcaacctc tgttgctgta acagaaatat tagatgaatt
    25321 aggagtaaag gtaagtggag ttccaactag ttcttatgat ttgcctgaaa gtactaagga
    25381 tgcggttaaa gttggtaatc ctatgaatcc agatttagaa ataataaaat ctttaaatcc
    25441 agatgttgta gtatctgtag ataccttagg agaagattat aaaaaactat ttactgataa
    25501 caatatacct agtgaattta tagatttaac tacattagaa ggattaaaaa caagtatatc
    25561 aactttaggt gaaagattta ataagacgga aaaagcaaat gaaatattaa atgagcttaa
    25621 agttaaggaa gatgagtttg tgaacttatc taaagaagaa aaaaagaatg ttttattagt
    25681 atttgcagca ccaggaagca tgatgattgc tacgccttca tcttacatag gaaatttagt
    25741 tgataagatt ggagcaaata acatagttaa agatgataaa aagccatttg tttcatatag
    25801 caatgaagaa atagtaaagc taaatccaga tatggtatta gttatgactc atggaatgcc
    25861 agaacaagct aaaaaaatgg ctgaagaaaa atttgcttca gatccagctt ggagtagaat
    25921 tgaggctgtt aaagaaggaa aggtctatta cttagaaaat gggtactttg gtatgagtgc
    25981 aaacctaaaa gtaacagaat ctttagataa gttaggtgaa ataatatatg gtgaaaaata
    26041 ataagttaaa aagcttatat ataggtttat caatattaat agttttaatt ttaatgataa
    26101 tttctttaaa gctaggaagt attgatataa gttttggtga actaataaaa ggtcttttaa
    26161 gtggaagtaa tgaaggaaat ataggaataa taaaagattt aaggatgcca agagttatta
    26221 tagctgcttt agttggagga aatcttgcct tggcaggggt attacttcag gcagtaataa
    26281 gaaatccctt ggctgatcct caagtaacag gaatatcttc tggagcatgc ttagtatcat
    26341 taatatttat aatatttatg ccagcttcag gatcaataag accaatagta ggtttctttg
    26401 gagggcttgt ttcttgtata gttgtgtatt taatggctta taaaaaagag ttagatacta
    26461 tgagaatagt ccttgcaggg gctgctataa atgctctttt aggagggcta tcacaaatat
    26521 taactatagg tagtggcata ggtgcaggga atatacaatc ttggataagt ggaagtttag
    26581 ctgccacctc atgggatgaa gttaaaattt tatttttata ttcaataata ggaatgggtt
    26641 ttgcattaat tttagctaag tcatgtaatg ttatagtttt aggaaataaa aatgcaaaaa
    26701 gtttaggttt taattcagat ttacaaatga ttctcataac agtggtagct gtatttttag
    26761 ggagtgtatc aacggctatt gtaggtatta tttcctttgt tggattagtt gtaccacata
    26821 tttgtagaat attaattggc tctgatcata gatatttgat tcctttttca atatttgttg
    26881 gaggagcatt agtcttactt gcagatacaa taggaagaat gctagtaaag ccatatgaaa
    26941 taccagcagg aataattatg gcagttattg gagcaccgtt cttcttatat ctactaagaa
    27001 ggagtgatat gtagatgatt agaggggaaa aattatcatt caattacaag ggagaaaaaa
    27061 atatcattaa tgatttaaat atagatataa aagagggaga aataacaact attattggcc
    27121 ctaatggaag tggaaaatca actttgctaa gtctattatg tgctttaaat aaaagtaaaa
    27181 ctggacatgt ttatttgggg gatataaaca tgaataatct taaatataag gatatagcaa
    27241 agattttagc aactgtccat caacaaaata gtgtgccaaa ggatataaag gttgaagaac
    27301 ttgttgccta tggtagatat cctcataaag gatattttaa aaataacaat aaggaagata
    27361 aaaacataat taaatgggct ttagaatgta caggacttga tgatttaaaa cataagggag
    27421 ttatgaattt atcaggggga gaaagacaaa gagcttttat atcaatggct ttagctcaaa
    27481 agcctaaaat tttattttta gatgagccaa caacttattt agatattttt catcaaatag
    27541 aaatattaga gattgtaaaa aagttaaata aggataatgg gctaactgtt gttatggttt
    27601 tacatgatat aaatcaagca attaaatata gtcataatat tgttgtcatg aaagatggaa
    27661 agattgtaaa agagggaaaa tcagagaatg taatagatga aaatttatta aaggaagttt
    27721 ataaggttaa tggtgttatt aacaaatgta aggaaactgg agagatttat tttataccca
    27781 aaactatttg ttaaataaat tttataaact tcagaaagat ggtgtaggaa ttatgatgat
    27841 agacaagagg cttttaaact tgtgtaagga atctaagaaa tacataggtt taactgtttt
    27901 aatgaattgg atttctgtaa tttgtaatct gatgacaata atagttattg gatttagcat
    27961 tcaggaactt tttagtttag ggaatattga aaacttaaat aaaaaaatat taattttagc
    28021 tgctctttta ggagtaagat tttgtgtgaa tctattaagt ggacacttct catataaatc
    28081 ttcagagatg gttaagatta ctcttagaga taagatatat gagaaacttt taaagcttgg
    28141 aatgagatat gataaggtag attctacatc ttcaattgtt caaatggcag ttgaaggggt
    28201 agaggcttta gaggtttatt ttagtaaata cttacctcag ttaatatatt ctattttagc
    28261 tcctttaact ttattttgtg ctttagcctt tatttcattt aaagcagctt tagtatttat
    28321 actttgtctg cctttaatac caatatcaat aatattaatt atgaaaatag caaagaaaat
    28381 attaaaggaa tattggggac agtattcaga tttaggaaat acattcttag aaaaccttca
    28441 agggcttaca acattaaagg tttttgatgt ggataatgaa agacatgaac ttatgaatga
    28501 agaggctgaa agctttagaa agataactat gaaagtttta agtatgcaat taaactcaat
    28561 aaatgttatg gatttaattg cttttggagg agctgctctt ggaagcatag tggctttatt
    28621 acaatttaga gctggaacag taagtcttgg aggaatgata gtaattttat taatatcagc
    28681 agaatttttc atacctctta gattattagg atcatatttt catattgcta tgaatggaat
    28741 agctgctgca gatagaattt tcaaagtttt agaagctgat gaaagcaagg tttcttcaga
    28801 tagtaatctt aatgaaatat caaatatctc tctttgtaat gtaagtttct cttatgatgg
    28861 aaaaagagaa gttttaaagg atatatcttt atcaataaat aaaggagact atgtagcaat
    28921 agttggtgaa agtggaagtg gaaaaagtac tatagcttca ttgattttaa ataactataa
    28981 ggcttcaaaa ggaaagataa ctttaaatgg agctgatata aataatatag atttttcaaa
    29041 gctttataat agggtatcta taatatcaac taacagttat atattcaatg gaacaattaa
    29101 agataattta ttaatagcta agaagaatgc aactttagaa gagataaatt atgcattaaa
    29161 aaaggctaat cttttagact ttgtgaattc taaaaaggaa aaattagatt tagaagttgg
    29221 tgaaggtgga tcaaagcttt caggaggaca aaagcaaaga cttgctttag ctagaactat
    29281 attagcaaat agagaagtaa taatctttga tgaagcaact tcaaatattg atgttgaaag
    29341 tgaaaatatg atttggaaat ctataaatga aatatcaaaa gagaaaacag taatagttat
    29401 ttctcatagg cttgctaatg ttaaaaatgc agatagaatt tatatgctag ataaaggaat
    29461 tctagttgaa gagggaagtc ataaggatct tatggaaaga aagaacaagt attatggtat
    29521 ggtaaattat caaaatgaat tagaaatagg aggtgttata taaatgagaa aatcaggttt
    29581 ttcaataatg aaaaggctta tagtagagct taagcctctt gcccctgtaa tgtttataac
    29641 agtatttatg ggagttttag gatttttagc agctatagga ataactgtat ttggaagtgt
    29701 agcaatatca agtttaatag atagtactat aagtttttca tttaaaacag ctcttatttt
    29761 aatgatagta cttggaatat taagaggacc tttaagatat ggagaacaat taagtggaca
    29821 ctatatagct tttaaaatat tagttatatt aagagataaa gtatttttag ctcttagaaa
    29881 acttgcacca gctaaattag agggaatgga aaagggaaat ttaatttcta tgataacttc
    29941 tgatattgaa cttttagaag ttttttatgc tcatactata gcaccaatca gtatagcaat
    30001 tataactaat accataatgg gattaatact tttaagtata aatgtaaagg tgggtatagt
    30061 tggaataatt ggttatatac taataggttt tggagttcct tatattagta gtagctttgg
    30121 aaaagaagct ggtgttcaat atagagaagc cttcggaaaa acaaattctt atattttaga
    30181 ttctcttaga ggaattaaag aggttttaat tttcaacaat ggtaatgaaa gaagagggaa
    30241 tataaaaaaa ttctctgaag aattaaatga gaagcaaaag gttataaaaa aacatgaggg
    30301 aataataaga gcctttagtg atttaatcat aatgttaaca atacttattg ctttatatgt
    30361 tgcttattta gaggttttaa aaggaactat gacaatagga gagttaataa ttgtggttac
    30421 tttaatagca tcttcctttg gacctgttgt agccttaagt aatctatcta acaatcttct
    30481 tttaactttt gcttcagctc aaagaatttt taatatctta gatgaaaagc cttctgtaga
    30541 agaagtttta ggagaaggaa tgaatgttgg aaacaacata gagtataagg atgtttcttt
    30601 tgcatataag ggaaaagaaa atgtattaaa agatgtaaac atagatataa aattaggtga
    30661 caagattgct gtaattggag aaagtggaac tggaaagagt acttttgtaa aattacttat
    30721 gagattctgg gatgttacaa agggtgaaat tagcttagga aataagaatg taaaagagat
    30781 tcagacaaaa gagttacgaa agaaaatggc cttaatggga caagaaacaa gtctatttaa
    30841 tatgagtatt gaagacaata ttaaaatagg aaagttagat gcaactttag atgaagtaaa
    30901 agaagctgca aagaaagcgg gaatacatga gtttattatt ggcttagagg atggatataa
    30961 aacaaaagta ggagaattag gaggaaactt atcttcagga gaaaaacaaa gaattggact
    31021 agctagagta ttcttaagag aaagtgaaat agtaattcta gatgaaccta caagtaactt
    31081 agatacttta aatgaaggaa taatcttaaa gtctataaga acatatttaa aagataaaac
    31141 acttatactt ataactcata gaaagtctac aactagggta tgtgaaaagg tatttagaat
    31201 taataaggga aatttaattc aatgtgaaga ggtgaaacat gagcttgctt aagatatttt
    31261 atgttatttt aggttttatt tccttaggat tagggttaat aggaataatc ttacctatat
    31321 tacctactac accattttta ttattaacag cattttgttt tgttaaagga tcagataagt
    31381 ttgataagtg gttcaaaggg acaaagatat ataaaaatca tttagaaagc tttgaaaaaa
    31441 atagggctat gactttaaaa actaaattaa ctatactact tacagctgat attatgttat
    31501 tatttccact tatactatca aagagtattc atctaaaaat atttatagta atattaatga
    31561 tgtttaaatt ctattatttt atctttagaa ttaaaaccgt aaaagtaggt gaggttgttg
    31621 aacagaatag ataaagaaaa agagataata acattaatga taaagcttta ttgcaagaag
    31681 aaacatggaa gtttaaatgg ggaactttgt aatgaatgta aggagttaga cgagtatgct
    31741 cataaaagat taacttattg taagttcgga aatgaaaaaa gttcttgtaa aaagtgtcct
    31801 attcattgtt ataaaaaaga tatgaaagaa aaagttaaag aggtaatgaa gttttcagga
    31861 cctagaatac ttatatataa tccaaaggaa tatataagac atatatttaa atagtttttt
    31921 taaaaagaga tgcatctaaa cagggaaaat atttcttgtt tggatgcatc tttttattta
    31981 tatattgaag atttatgtgg taagctaaaa atatattttt aactt
//
LOCUS       DAEVGI010000014        30487 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEVGI010000014 DAEVGI010000000
VERSION     DAEVGI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 30487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30487
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.014"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            137..1363
                     /locus_tag="K8M77_000623"
     CDS             137..1363
                     /locus_tag="K8M77_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7085140.1"
                     /translation="MGKRTKILATVMAATMLFAGSLVGCGEKADSGSTDGSGKELTVW
                     SHLTTPEVEELNKIASKWGEENGVKVKVVEDKSEMQAYIQAANSSKGPDIMFGLAHDN
                     LGTFQKAGLLAEVPEGIINDSDYASHQVLDAVTIGGKRYAVPIAQETSALFYNKDKVK
                     EVPKTMEDLVKVAKDGVGFEYDINNFYPTYGFIAADGGYVYKDNNGTLDPTDIGLGTP
                     GAIKGYQFVQDLVQKDKLMPADITGDIAKGDFLSKKSGFYISGPWDISAFKDGGVNFG
                     VAPMPTLFEKQVPTFLGVQTAFVSEKSQNKDLAWKLVKYLSENSGDVLLSKGNRIPVL
                     NKYLDSADFKNNEYMSAFAEQAKFATPMPNIPEIQAMWGPAGANLQLLTSGQVTPEKC
                     AEMTVEQIKQGISQQK"
     gene            1450..2403
                     /locus_tag="K8M77_000624"
     CDS             1450..2403
                     /locus_tag="K8M77_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI7085141.1"
                     /translation="MQVTSDRRADKKQKRLKDTIKAMPYLLPALISIIIFTIIPIVYT
                     VVIAFTDYTMYSQGHIKFVGFANFIEVLTGPFKEVFLPVFVWNIIFAVVSTAGTFFLG
                     LIVAMAVNNPNIKEKSVYRAILIIPWALPATVAILSWQGLLNGSYGAINNLLLNLHLI
                     SNPIPWLTDPTWARVALIIVNIWLGFPYMMNVCLGALGAIPDSYYEAADVDGASKWLQ
                     FRKITLPSLAQISYPLLISSFAFNFNNFGSAFLITKGGPPRMSTQFAGYTDILASVNY
                     KLSTQFGRFEIASAISIIIFLILGTISYYQMKLSGQFEEVE"
     gene            2405..3298
                     /locus_tag="K8M77_000625"
     CDS             2405..3298
                     /locus_tag="K8M77_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI7085142.1"
                     /translation="MQSKVQKKSGIVTTSELKYERKLRPAERRIAWISRIFIWFMLLV
                     VLFPVFAVVSASMAKGEVFTQTTLIPSAFTLENYSKVLTETNFLIWVKNSLIICVIVS
                     VLQLLLTVPMAFAFSRLKFWGRKNGLMMLLLLQMFPAAMSLPAILALAYRLDGMDHII
                     TLIIVQAGAGAFNIWLLKGAIDGIPKELTEAAYVDGASTFQTFTMIILPLLRNMLLVI
                     FLFTFIGAYSEFVFASALLKSPESLTLAVGMQQFITNNFSANWTQYSAAAIMASIPIV
                     AFATIAQKYMAKGLTAGSVKG"
     gene            3593..4606
                     /locus_tag="K8M77_000626"
     CDS             3593..4606
                     /locus_tag="K8M77_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7085143.1"
                     /translation="MNIKDIARLAGVGVSTVSRVINDHPDVKDETREKILKIIKESNY
                     IPNNSARILKKNNTNNIGVLVKGVFNPFFSEMINIIGNRINEAGYTMILQQNDYATED
                     DVDNLIAFVKEKRLQGIICLGGNFLNINDESFQFLDIPVVLTSVNTLSKESKSKFSSI
                     GIDNVLAAKASIQYLIDKGHRNIGILLGEKNDVGISGLRLEGYKKALEENNIPYSEEN
                     VFIGDYDYSGAYRVTKEIINNRKDITAIFSISDIMAVGAAKSVIDQGLQVGEDISIMG
                     FDGMDISKYYNPGITTVKQPKKNMANNSIDLLLALLAKKEEHKHIIFETKIIERESCK
                     KVV"
     gene            4630..6777
                     /locus_tag="K8M77_000627"
     CDS             4630..6777
                     /locus_tag="K8M77_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HBI7085144.1"
                     /translation="MRINCINNHITKYTFGTPFNTESVVLKEGSEIENQGLKYLNFEN
                     NTFTLKLKEEDIVYGLGENVRGLNKRGWRYESFCTDDFSHTPEKKSLYGAHNFLMIYG
                     EKNFGVFIDYPGRVKFDVGYSRSDELKIEIDDENFALYLIEGDTLKEIAKSFRELIGE
                     SYIAPKWAFGAQQSRWSYKDSKEVLEVLDNFNESELPLDCIYLDIDYMEDFKNFTINK
                     EAFPDFENFVKEVKEKGVRLIPIIDAGCKIEEGYDVYEEGVKNGYYCLDEEGKPFVAA
                     VWPGRVHFPDFLNKDARLWFGNKYKVLTDKGIEGFWNDMNEPAIFYSEKRLNEAFEKI
                     SEAKGKNLGIYDYFDVKDTFPRLQNSMEDYQSFYHRVGNEKIRHDKVHNLFGFNMTRA
                     AAEGLENIDENKRFLLFSRASTVGMHRYGGIWTGDNMSWWEHIKLNLQMMPNINMCGF
                     IYTGADTGGFGGDTTEDLVIRWSQFSMFTPLFRNHSALGTRHQEPYSFRGESVKVLKN
                     ILELRYVMVPYLYSEYMKAVLNNEMYFKPLTFEYSDNFVKRVEDQMLLGDSLMVAPIY
                     EQNALGRYVYIPEEMLLWRARKYDDGDYEVLEKGHHYVEAKLNEVPIFIRKNKIVTMA
                     SKPRKRVEEIDTSELNLIAFVCDKAEYIYYNDDGITKNYKNGEYEELKIKITKVQSDY
                     EVLVENKNSRVKKLNIKIFDLNKNMIEKEILIQ"
     gene            6799..8292
                     /gene="malQ"
                     /locus_tag="K8M77_000628"
     CDS             6799..8292
                     /gene="malQ"
                     /locus_tag="K8M77_000628"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBI7085145.1"
                     /translation="MRRASGIIMHIASLPGKYGIGTFGKEAFEFVDFLKKAGQGYWQI
                     LPLGPTSYGDSPYQSFSAFAGNPYFIDFDILNKEGLLNKKDYQGINFGNDPEKIDYAL
                     LFDKKMRVLRIAYEKSLDKNKEEIEKFREENKLWLEDYALYMAIKNENELVSWQEWDE
                     KLRLRDKKILEEYKVKLEKEINYWVFLQYNFFKQWNELKEYANSFGIKIIGDMPIYVA
                     EDSADVWANPKAFLLDENNIPKKVAGCPPDAFSETGQLWGNPIYDWNYMDDTGYSWWI
                     DRVRESFKLYDILRIDHFRGFEAYWQIPYGDETAVNGEWVKGPGIKLFNAIKEELGEV
                     NVIAEDLGYLTQEVIDFRNETGFPGMKVLQFAFDSREESDYLPHNYPVNSIAYTGTHD
                     NDTFRGWFEVTGNREDVEYSKKYLKLTEEEGYNWGFIRGVWSSVSHTAIALMQDFLNL
                     GNEARINYPSTLGGNWQWRVKDDALTDELAEKIYDITKLYGRVNINE"
     gene            8285..10648
                     /locus_tag="K8M77_000629"
     CDS             8285..10648
                     /locus_tag="K8M77_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBI7085146.1"
                     /translation="MNKEQIKRGIERYLKVKYGKSSIKDAKDFEIFNALSLTLLEGIV
                     DDWNHTEELYEKEKNAYYFSAEYLMGRALGNNLISLGLYNEVKEVLDELGFDLNRIEE
                     IEEDAGLGNGGLGRLAACFMDSGASLEVPLKGYGIRYNNGLFSQYFEDGFQKEDVDSW
                     LKYGEPWSIRKEDEFVTVDFEDMTVKAIPYDTPIIGYASKNINTLRLWKCEPVKEFDF
                     KLFNEQKYDEALELKNRAEDISRVLYPNDSNRDGKLLRLRQQYFLVSASLKDIIRKHK
                     EVFGNVTEDFAKMHAVQLNDTHPVLAIPELIRLLVDEEGFTFEEAYNVASKTFAYTNH
                     TILAEALEKWDVDLIEELFPRILEIIEKIDAEFIVSLEEKGYSEEEIEEFRIVNDGKV
                     RMANLAIHVGFAINGVAKLHTDILKNIELKNWYELYPEKFQNKTNGITPRRWLRLCNQ
                     ELSALITELLGSEDWVKNLDLLKGLEKYKDDEEVLKRFMDIKHTKKEQLAAYIKEHEG
                     VELDPDSIFDIQIKRLHEYKRQLLNTLYILDLYYRLKENPDMDIPKVTFIYGAKAFPG
                     YKRAKSIVKLTNEVAKLINNDESIKGKIKVVFVENYRVSYAEKLFPAADVSKQISTAG
                     KEASGTGNMKFMLNGAPTFGTNDGANVEIVRESGVDNNFIFGLEVEEIEELKGKYNPV
                     DYYESDADLKRVLDSLVDGTFDDGGNGDFEDLYNSLLEEGDQYFLLADFKAFKDTEDK
                     VFEAYKDKLGWAKKAFENTCNAGMFSSDRTIQEYCDDIWGIKKCTNK"
     gene            10991..13009
                     /locus_tag="K8M77_000630"
     CDS             10991..13009
                     /locus_tag="K8M77_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HBI7085147.1"
                     /translation="MKFGKKDWTSYDRGIEKEWMLTNGISGFCGGTAIGANCRKYQGL
                     LVACTNSPEERYMILSNLNEEINIDGKLHQLSSCKYTDKIVDGFKNLQGFYYDGLPHF
                     RFFVDGVVINKKIAMEYGKNTVVIEYDILNGSKESKINIKPLFTFRNPGICSGEENLK
                     FSKTVDKNKIQLTPQLNKDINIRFFTYGGKILDKTDKIVDKIYYDVDKSTGDKFVDKY
                     YIPGTVEINLNPNERKKVYLLCTIENEENFDCEKIIENEEKRINNLKNTFADSRILAK
                     YLPIAGDQFIVNRESIKGKTILAGYPWFLDWGRDAMIAINGLTLSTGRLEDAKDIIRS
                     FSLYEKDGLIPNMFPGKGQEPLYNTVDASLWFINAVYNYLLYANSYEALEFVEKEVYK
                     TIKNIIKAYKEGTKFSIKMDEEDYLINAGSGLDQVTWMDVRVNGIVVTPRHGKPVEIN
                     ALWYNALKIAALLKNKFEKEEKNEYEELAKKVKDSFTKTFWNEDRKYLYDVVNNHEKD
                     DSLRPNQIWAVSLPFTMLDREKEKHIVQKVFEELYTPYGLRSLSRNNKDYHGIYIGKL
                     FDRDMAYHQGTTWAFPLGGFFTAYCKVNDYSKEAVDLIDSLMRDMEDHIKDQCLGSIA
                     EIFDGDNPYKARGCYAQAWSVGEMLRVYYEDILGNYKKLKKVHKDIFI"
     gene            13125..13964
                     /locus_tag="K8M77_000631"
     CDS             13125..13964
                     /locus_tag="K8M77_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBI7085148.1"
                     /translation="MKTSLNIENKINMITEMRGDSVFQVLEYDSLKGGNSLELAVKLN
                     QMKEANIKLRQVRIILEDSSVNIEKGALSYMRGPISMKNKIGGPIGLGKKLFSSKVTG
                     ESTFKPKYEGTGDIFLEPSFGHYALIELEDDEIIVDDGLFYACESSVEITAHMNKSLS
                     SMVLGNEGVFQTKLSGNGIAVLEIPVPEEEIFKCKINNDVLKVDGNFAILRTGDIDFT
                     VEKSSKSLVSTATGGEGFLNVFRGTGEVWLIPTKSVYEKLEKGWNSLKPLTDPKGSSN
                     TKI"
     gene            14103..14402
                     /locus_tag="K8M77_000632"
     CDS             14103..14402
                     /locus_tag="K8M77_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpR-like protein YerC/YecD"
                     /protein_id="HBI7085149.1"
                     /translation="MNGVQSKLKGKDLDFLFEAILRLKDIDECYNFFEDICTISELKA
                     LAQRLHVAKMLKEKKTYTEIAEETKASTATISRVNRCLNYGANGYNNILNRLNEK"
     gene            14468..14785
                     /locus_tag="K8M77_000633"
     CDS             14468..14785
                     /locus_tag="K8M77_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBI7085150.1"
                     /translation="MPNLSCGVDNCAYNQNDMCSLNSIKVNGSFANHSEATCCGSFVE
                     KGGFTNAAVDMGPAEPATSVACEAEKCCYNKNCKCHAESIDISGDYAQHQDNTECSTF
                     RQE"
     gene            14955..15647
                     /locus_tag="K8M77_000634"
     CDS             14955..15647
                     /locus_tag="K8M77_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085151.1"
                     /translation="MNILLVDDEEKIIEVLEAYLKKEGFSVFSCSNGEEALKIFDNNK
                     IDLILLDLMLPDLSGEEVCKVIRAKSCVPIIMITAKTEEEDLLEGFDIGADDYVTKPF
                     SVKQLIARIKAIIRRSTPCPNEGSDILVFNNGELKINVDTREVWVRDELITLTSTEFN
                     ILLCLSSYPKKIFTRDEIIELVLGDKSDSFDRVIDSHIKNLRGKIEENTRKPKFIVTV
                     YGVGYKFEGKKI"
     gene            15628..17031
                     /locus_tag="K8M77_000635"
     CDS             15628..17031
                     /locus_tag="K8M77_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7085152.1"
                     /translation="MKVRKYKSLNFKVIVPSIVIIIIVTLLTVVISKFYFDKKFGDYI
                     MIKNQNTVQNILMELSEQYSDNKWDYKGMEKISYNALDKGIIVALYDKEDKEIMNIEK
                     NSKDKCNRIMNFIKSSMEGKYGSTTSQFEPVYYPLIKSGEKIGEVRIKFYGPIFYMQN
                     ELVFLDIVNKIILGIGVLLILASTVIGFIISRSITRPINKLMTKAKYISKGEYDKKIE
                     INTDILEINDLINSINNLSQSIKEQENIRKRLTGDISHELKTPLTNIQSHLEAMIDGI
                     WEPTEERLLSVKEEAERLSSLVSDMQKLNKYDESSIKLKKDNVNISDIICFVIFQFSN
                     LAKSKNIKIEYEKKNINLYCDKDKITQALVNILSNAIRYSNEGSTIFIEEKLKDNKVI
                     ISIEDQGIGISEEDLKYVFERFYRADKSRTRATGGTGIGLTIVKSIVSSHGGEVKLES
                     KLGEGSKFTIILPKKDI"
     gene            complement(17295..18587)
                     /locus_tag="K8M77_000636"
     CDS             complement(17295..18587)
                     /locus_tag="K8M77_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HBI7085153.1"
                     /translation="MTTNESNYIDENKNPIETEDFAKALAAEEENSCKRKKEVIEKIR
                     SLAKSAQNWKEANKEFNALIEEFNNIYYYDQETENTLKNELREAKNEFYTARKEFFEK
                     ANEGFKENAEKKEDVIKRLEALVYTSDIKASDMLTKSLSEEFYAIGFAGKDLNTELYD
                     RFKKARNAAQETRKAAMEGLREEFGNKADRKREIISELKALVNNENWKEATEKFNALC
                     NEFKGIGFSGKEGNEEISLGYKEAKDAFFAKRQEFFDALKAENKVNIEKREALIEELK
                     KLYENDSWKEASAKVKEISDEFFKIGFCGKEENEKLINEFKEIRDGFYAARQEYFDKI
                     NAARANKQKDFLNSLLKNKDEFVKKIKKYISQDEERLSDFTNRLFNVRPGNNSLETIE
                     KYQEIVEDIKGRIESNKAKVKEVQNEMHEIRNELNNLK"
     gene            19058..20404
                     /locus_tag="K8M77_000637"
     CDS             19058..20404
                     /locus_tag="K8M77_000637"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBI7085154.1"
                     /translation="MSRLFGTDGVRGIANTELTAELAYNLGRAGAYVLTEGTHKPKIL
                     VAKDTRISGDMLEAALVAGILSVGAEALCLGVVPTPAVAHLTRAYGADAGVMISASHN
                     PVEYNGIKFFDDKGYKLSDDLEDEIQRVIESGFENVPSPTGANLGREIIEKAALEDYI
                     SFAKDTIGISLEGLRVALDCANGASHEAAVRAFRELGAEIFVINDNPDGTNINENCGS
                     THPEELMEYVVKKKCHMGFAFDGDADRCLAVDEQGNLVDGDFILTICAKYLKELGRLK
                     DDTLVVTVMSNLGLMIACKNEKINTAVTKVGDRYVLEEMLAKGYSLGGEQSGHIIFLD
                     HNSTGDGLVTALQVASIVKRTGKSLFELKNVMKVLPQVLVNAKVPNNMKNIYEEDEEI
                     IAEIKKMEAALDGCGRVLIRPSGTEPLVRVMLEGENQAEIDEMAHNLANMIEAKCN"
     gene            complement(20554..21078)
                     /locus_tag="K8M77_000638"
     CDS             complement(20554..21078)
                     /locus_tag="K8M77_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI7085155.1"
                     /translation="MSYTTEKQPQGKALDIKGFLREALISELVAINGYSKHIDEISLV
                     EIKELLHHIMEEEKRHYGQFLEALRRYDKEEFEVYVESVDHVEIKDKPLKKTYHENKK
                     LTAYSILNKIRDDIKGEYEAILLYDDLINKIEDKCLVKMIRDITKEEKEHVEELTLAL
                     MRLDSDKYGPIEEN"
     gene            21865..23697
                     /gene="glmS"
                     /locus_tag="K8M77_000639"
     CDS             21865..23697
                     /gene="glmS"
                     /locus_tag="K8M77_000639"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBI7085156.1"
                     /translation="MCGIVGYVGQKKATDILVEGLSKLEYRGYDSAGVAVLEDNKIKA
                     EKHKGRLANLEGMLNENPIEGGIGIGHTRWATHGEPSDVNSHPHLNNKETIAVVHNGI
                     IENYNELRNWLMEKGYEFKSETDTEVIPNLVDFYYKGDLLDAVMEATKHMEGSYAIGV
                     ICNDEPEKLVAVRKDSPLIVGLGEKEYFIASDIPAVLNYTREVYLLEDKEFVVLTNDG
                     VTLFDEEKNPVEKEVYHITWNVDAAEKGGYEDFMLKEINEQPKAIKDTMTSRIMEGKE
                     VTLDDISITKEYLDNVDRVYIVACGTAYHAGVIGKYAIEKLVRIPVEVDIASEFRYRD
                     AVITDKTLIIVLSQSGETADTLAVLRDGQAKGARVLAVTNVVGSSVSREANDVLYTWA
                     GPEIAVASTKAYVTQLIAMYTLALHFAELKGSKSVEEIEEIKKAMLELPEKVEEILKN
                     TDLIKEFAVKASTEKDLYFLGRGMDYGVAMEGSLKLKEISYIHSEAYAGGELKHGPIA
                     LIEKDIPVISLLTQRELMDKMISNVQEVVTRGANVLGVCFKGDMEESKRKMFEGLIEI
                     PETLSLLSPVLSVVPLQLFSYYVAKAKGFDVDKPRNLAKSVTVE"
     gene            23714..23884
                     /locus_tag="K8M77_000640"
     CDS             23714..23884
                     /locus_tag="K8M77_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085157.1"
                     /translation="MNNESLLKLLAEYKETKKCLETGLNWLEEKDYAKGKLDIVNVII
                     RDLEAAIGAERI"
     gene            24097..24909
                     /locus_tag="K8M77_000641"
     CDS             24097..24909
                     /locus_tag="K8M77_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI7085158.1"
                     /translation="MKNFQENNILEDKKVCINENEEAWNKETYNAWVKRFGTAKEAAE
                     KIKSMPEKKLNVLLDKFGDVKGKKIFNLMGSNGNKAVALALLGADVTVVDFSEGNRRY
                     ALDLAEACGVKINFILSDVLKMPKEVMSGDYDIVFAEMGILHYFSDLSPFMNVIHSLL
                     KDGGKAVLRDFHPVSTKLITSRGSTAKVRKHKVTGDYFDTSLEEKEVAYSKYLEEGKV
                     EKVFLRKWNLGEIVTAVANTGLKIESLTEEPNLSSDVFDKGIPKTFTIVARK"
     gene            25135..25713
                     /locus_tag="K8M77_000642"
     CDS             25135..25713
                     /locus_tag="K8M77_000642"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBI7085159.1"
                     /translation="MDKGELRKEIKLKRENIDRNTKLMADEKIRKTLLESEIYKNSKV
                     VFIYINMDSEVNTVEIIKELLASDKKVAVPKVIPVSLKERQMKALKIDSLLQLNESGA
                     FGILEPSIECKDISEEVDLIIIPGLAFDLKGNRLGYGGGFYDRFLSKYPNSKRVALCY
                     DFQVFDEIPNEFFDEKVDLIISEEKIISLKKS"
     gene            25809..27503
                     /locus_tag="K8M77_000643"
     CDS             25809..27503
                     /locus_tag="K8M77_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBI7085160.1"
                     /translation="MKFEDFKYERPNIEKDREDIRSFIKELEEVKDFNGAKEVIGKIN
                     KIRNNTSTMMALSEVRHTINTEDEFYNEESDFWDENSPLIEEVNNEFYKALLSSPFKD
                     QIGDYYGETIIKEAEYSQKSFSKEVIEDMQLENKLGSQYQKLIASAKIEFDGEERTLA
                     QLVPFVTSEDREVRKRASEAKYNFFVKHEDEIDDIFDKLVKVRTKIAKKLGFNNFVEL
                     GYVRMRRYDYNKEMVENFRKQVEEFIVPIDSKLYERQAKRLGLETLKYYDEKFEFLSG
                     NPTPKGDSKWIVENAKVMYSELSKETKEFFDFMVENDLMDLVAKKGKAAGGYCTNFPN
                     YKAPFIFSNFNGTAGDVDVLTHEAGHAFQNFRSSWIEMQECQWPTMESCEIHSMSMEF
                     FTWPWMYLFFKEDTDKYKFYHLGNAIKFIPYGVTVDEFQHFVYENPECTPKERKDAWR
                     RIEKKYLPHKNYDECDFLERGGWWFQQNHIFLSPFYYIDYTLAQICALQFWKKDRENH
                     EKAWEDYLNLCNIGGTKTFLGLLKYANLKSPFENGCVESIVGVVDEYLESIDDSKF"
     gene            complement(27958..29889)
                     /gene="thrS"
                     /locus_tag="K8M77_000644"
     CDS             complement(27958..29889)
                     /gene="thrS"
                     /locus_tag="K8M77_000644"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBI7085161.1"
                     /translation="MIKITLKDGSIKEVEAGLSIFEIAQSISQGLARNACCGILNGKV
                     EDLRFIVNEDSSLEICTFDSKEGQHAFNHTASHVLAAAVKRLFPQDKLAIGPSIDNGF
                     YYDFDTEKSFSADQLNKLEEEMKKIIKENPEIKRFELPRNEALELMKDEPYKVELIND
                     LPEGEVISFYQIGDFVDLCAGPHLMAVKPIKAVKLLRSTGAYWKGDEKNKMLSRVYGT
                     AFPKKSELDAYLEALEEAKKRDHNKLGRELGIFTTDENVGQGLPLLMPKGARIIQTLQ
                     RWIEDEEQRRGYVLTKTPLMAKSDLYKISGHWDHYKDGMFVLGDEEKDSEVFALRPMT
                     CPFQYAIYNSTQHSYRDLPVRFAETSTLFRNESSGEMHGLIRVRQFTLADGHIVCTPE
                     QLEDEFKNTVDLVKYVMETLGIADDITYRFSKWDPNNTEKYINDPEAWENTQNIMREI
                     LNHLNIDFTEADDEAAFYGPKLDIQFKNVHGKEDTIITIQIDFALAERFGMYYIDKDG
                     EKKRPYIIHRSSIGCYERTLAMLIEKYAGALPTWIAPVQAKVLPLSDKYADYANEVVE
                     ELRRRGVRVEADHRAEKIGYKIREARLERTPYILVVGEKEAENKEVSVRSRKNGEEGA
                     MPLADFVNRIVLEIANREN"
     misc_binding    complement(29971..30190)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        11661 a         3380 c         5418 g        10028 t
ORIGIN      
        1 cgaaatgttt ttaaaaagat tttaaataat tagcattatg aaatattttt ttataatatt
       61 ttggtatcgt ttccgagagt gagaatgtaa atttatagct ttttagctaa ttaaaattta
      121 tgagggggat tttataatgg gaaaacgtac aaaaattcta gcaactgtaa tggctgcaac
      181 aatgttattt gcaggatcat tagtaggatg tggagagaaa gctgattcag gtagtactga
      241 tggttcagga aaagaactta cagtatggtc acatttaact actccagaag tagaagaatt
      301 aaataaaata gcttcaaaat ggggagaaga aaatggagtt aaagttaagg ttgtagaaga
      361 caaatcagaa atgcaagctt acatacaagc tgcaaatagt tcaaagggac cagatataat
      421 gtttggacta gctcatgata acttaggaac attccaaaag gctggacttt tagcagaggt
      481 tccagaagga attataaatg attctgatta tgcatcacat caagttttag atgctgtaac
      541 aataggtgga aaaagatatg cagttccaat agcacaagaa acatcagctc ttttctataa
      601 taaggataaa gttaaagaag ttccaaaaac catggaagat ttagttaagg ttgcaaaaga
      661 tggagttgga tttgaatatg atattaataa tttctatcct acttatggat ttatagcagc
      721 agatggagga tatgtttata aagataataa tggaactctt gacccaactg atatcggatt
      781 aggaacacca ggagctataa aaggatatca atttgttcaa gatttagttc aaaaagataa
      841 attaatgcca gctgatataa ctggagatat agcaaaggga gatttcttat ctaagaaatc
      901 aggattttat atttcaggac cttgggatat atcagcattt aaagatggag gagtaaattt
      961 tggagtagct ccaatgccaa cattatttga aaaacaagta ccaactttct taggtgttca
     1021 aactgctttc gtaagtgaaa aatctcagaa taaagattta gcatggaaat tagttaaata
     1081 cttatcagaa aattcaggtg atgtattgtt aagtaaaggt aacagaattc cagtattaaa
     1141 taaatactta gatagtgcag atttcaaaaa taatgagtat atgagtgctt tcgcagaaca
     1201 agctaaattc gctacaccaa tgcctaatat accagaaatt caagctatgt ggggacctgc
     1261 tggagctaac ttacaattat taacttcagg acaagttaca ccagaaaaat gtgctgaaat
     1321 gacagtagaa caaattaaac aaggtatatc tcaacaaaaa taattaagaa gtttttatag
     1381 ggggatgtaa attccccctt atttatagaa gtttatatta aaaagaggta tttgaggagg
     1441 ctagatataa tgcaagttac gagtgataga agggcagata aaaagcagaa aaggttaaaa
     1501 gatacaataa aagcaatgcc atatttgtta ccagcattaa tatcaataat tatatttact
     1561 attattccta ttgtttacac tgtagtaata gcatttactg attacacaat gtattcacaa
     1621 ggacatatta aatttgtagg gtttgcaaat tttatagagg ttttaacagg accatttaaa
     1681 gaagtatttt tacctgtttt tgtatggaat ataatttttg cagtagtatc aactgcgggt
     1741 acatttttct taggattaat agttgccatg gcagtaaata accctaatat aaaagaaaaa
     1801 tcagtttata gggcaatact tataattcca tgggctttac cagctacagt agcaatactt
     1861 tcatggcaag gtcttttaaa tggaagttat ggagcaataa ataatttatt attaaattta
     1921 catttaatat caaacccaat tccatggtta acagatccaa catgggcaag ggtagcactt
     1981 ataatagtta atatatggtt aggattccct tatatgatga atgtttgttt aggagcctta
     2041 ggagcaattc ctgatagtta ctatgaagca gcagatgttg atggtgctag taaatggctt
     2101 caatttagaa aaataacatt accatcatta gctcaaatat cgtatccact attaatttca
     2161 tcatttgcat ttaactttaa taactttgga tcagcattct taatcactaa aggtggtcca
     2221 ccaagaatgt ctactcaatt cgctggttat acagatattc ttgcatctgt aaactataaa
     2281 ttatcaacac agtttggtag atttgaaata gcatctgcaa ttagtataat aatattctta
     2341 attttaggaa ctatatcata ctatcaaatg aaattaagtg gacaattcga ggaggtagaa
     2401 tagaatgcaa agtaaagtac aaaaaaagag cggaatagta actacctcag aacttaaata
     2461 tgaaagaaaa ttaagacctg ctgaaagaag aattgcatgg atatcaagaa tatttatatg
     2521 gtttatgtta cttgtagtat tattccctgt atttgcagta gtttctgcat caatggctaa
     2581 aggagaagtt tttactcaaa caactttaat tccatcagca tttacattag aaaattactc
     2641 aaaagtttta acagaaacaa attttttaat atgggtcaaa aattcattaa taatatgtgt
     2701 aatagtatca gtactacaac ttttattaac agtacctatg gcttttgctt tctcaagatt
     2761 gaaattctgg ggaagaaaaa atggattaat gatgttatta ttgcttcaaa tgtttccagc
     2821 agctatgtca ttaccagcaa tattagcact tgcttataga ttagatggta tggatcatat
     2881 aattacattg ataatagtac aagctggtgc tggtgcattt aatatttggc tacttaaagg
     2941 agctatagac ggtattccaa aagagcttac agaggcagct tatgttgatg gagcatcaac
     3001 ttttcaaact ttcacaatga taatattacc tttactaaga aacatgcttt tagtaatatt
     3061 cttatttaca tttataggag catatagtga atttgttttt gcatcagctt tattaaagag
     3121 cccagaaagt ttaacacttg ctgtaggtat gcaacaattt attacaaata atttctcagc
     3181 taactggact caatattcag cagctgcaat aatggcttca attccaatag ttgcatttgc
     3241 tactattgca caaaaatata tggctaaagg actgactgct ggatcagtta agggctaata
     3301 tagtataaat tttatgttgg ataaagctta aataccttaa atttaataaa tacttcttat
     3361 aggataaaat atacttatgg attttgtgga aggaaaatta ccttccacaa tccattaaaa
     3421 tatagtggta tctaagatta ttaaattaaa aatataaaaa attagtatat ttatctaaac
     3481 tcttgtaagt attattgcta atgttttcaa agcagtttat aattaaaatg gtatcgttac
     3541 cgaaactatt tttaagtttt ttaagattat agggaaaatc aggaggagtt atatgaatat
     3601 aaaagacata gcaagacttg ctggcgttgg tgttagtaca gtatcaagag ttataaatga
     3661 tcatccagac gtaaaggatg aaacgagaga aaaaatctta aaaataatca aagaaagtaa
     3721 ttacatacct aataatagtg ctaggattct taagaaaaat aatacaaata atataggagt
     3781 tctagtaaag ggggtattta acccattctt ttctgagatg ataaacatta taggtaatag
     3841 aataaatgaa gctggctaca caatgatatt acagcaaaat gactatgcca cagaggatga
     3901 tgtggataat ttaatagctt ttgttaaaga aaaaagactt caaggtatta tctgcttagg
     3961 aggaaacttt ttaaacataa atgatgaaag ttttcaattc ttagatattc cagtagtttt
     4021 aacctctgtt aatacacttt ctaaagaaag taaaagtaag ttttcatcaa taggaataga
     4081 taacgtatta gcagctaagg cctcaattca atatcttata gataaagggc atagaaacat
     4141 aggaatttta ttaggagaaa aaaatgatgt tggaataagt ggattaagac ttgaaggcta
     4201 taaaaaagct ttagaggaaa ataatatacc ttattcagaa gaaaatgtat ttataggtga
     4261 ttatgattat agtggagcat atagagtaac aaaggaaatt attaataata gaaaggatat
     4321 aacagctata ttttcaatat cagatattat ggctgtaggg gcagcaaaat cagttataga
     4381 tcaaggattg caggtaggag aagatatttc tattatggga tttgatggaa tggatataag
     4441 taaatattat aatccaggaa ttacaactgt aaagcagcct aagaaaaata tggctaataa
     4501 tagcatagat ttattattag ctcttttagc taaaaaggaa gagcataagc atataatttt
     4561 tgaaacaaag ataatagaaa gagagtcatg taagaaagtg gtatagtact tataattggg
     4621 ggagatatta tgagaattaa ttgtattaat aatcacataa ctaaatatac ttttggaact
     4681 ccttttaata ctgagtcagt tgttttaaaa gaaggttcag aaatagagaa ccaaggatta
     4741 aagtatttaa attttgagaa taacactttt acattaaaac ttaaagagga agatatagtt
     4801 tatggactag gtgaaaatgt aagaggatta aataaaagag gttggagata tgaaagtttt
     4861 tgtacagatg atttttcaca tactccagag aaaaaaagcc tttatggagc tcataacttc
     4921 ttaatgatat atggagaaaa aaattttggg gtatttatag attaccctgg aagagtgaag
     4981 tttgatgttg ggtatagtag aagtgatgag ttaaagatag agattgatga tgaaaacttt
     5041 gctttatacc ttattgaagg agatacttta aaggaaatag caaaaagctt tagagagctt
     5101 ataggtgaaa gttacatagc tcctaaatgg gcctttggtg ctcaacaaag tagatggagt
     5161 tataaggata gtaaagaggt tcttgaagtc ctagataatt ttaatgaatc agaacttcct
     5221 cttgattgta tatatcttga tatagattat atggaggatt tcaagaactt taccataaat
     5281 aaagaagcct tccctgattt tgaaaacttt gtgaaggaag ttaaagaaaa aggagtcaga
     5341 cttattccaa taatagatgc tggatgcaaa attgaagagg gatatgatgt ctatgaggaa
     5401 ggtgttaaaa atggttatta ctgtttagat gaggaaggaa aaccctttgt agcagcagtt
     5461 tggccaggga gagttcattt tccagacttc ttaaataagg atgcaagatt atggtttgga
     5521 aataaatata aagtacttac tgataaagga atagaaggct tttggaatga tatgaatgag
     5581 cctgcaatat tctattctga aaaaagatta aatgaagctt ttgaaaaaat atcagaagca
     5641 aaaggaaaga atttaggtat atatgattat tttgatgtta aggatacatt tccaagactt
     5701 caaaatagta tggaagatta tcaaagtttc tatcatagag taggaaatga aaaaataaga
     5761 catgacaagg ttcataatct ttttggattt aatatgacaa gagcagcagc tgaaggtctt
     5821 gaaaatatag atgaaaataa gaggttttta ctattttcta gagcatcaac agttggtatg
     5881 catagatatg gaggaatatg gactggagat aacatgtctt ggtgggaaca cattaagtta
     5941 aaccttcaaa tgatgccaaa tattaatatg tgtggattta tctatacagg agctgatact
     6001 ggcggatttg gaggagatac aacagaagat ttagttataa gatggtcaca atttagtatg
     6061 tttacacctt tattcagaaa tcactcagct ttaggtacaa gacatcaaga accatattca
     6121 tttagaggtg aaagtgtcaa agtattaaaa aatatattgg agttaagata tgttatggtt
     6181 ccatatcttt atagtgagta tatgaaagct gttttaaata atgaaatgta ttttaaacca
     6241 ttaacatttg aatatagtga taactttgta aaaagagtag aagatcaaat gcttctaggt
     6301 gatagtttaa tggtagcacc tatatatgag caaaatgctt taggaagata tgtatatatt
     6361 ccagaggaaa tgcttttatg gagagctaga aaatatgatg atggagatta tgaggtttta
     6421 gaaaaaggac atcattatgt tgaagctaaa ttaaatgaag ttcctatatt tataagaaaa
     6481 aataaaatag taacaatggc aagtaagcca agaaaaaggg tagaagaaat agatactagt
     6541 gaattaaatc taattgcttt tgtttgtgat aaagctgaat acatttacta caatgatgat
     6601 ggtatcacta aaaattataa gaatggtgag tatgaagagc taaaaattaa gattacaaaa
     6661 gtacaatcag attatgaggt attagtagaa aataaaaatt caagagttaa aaagttaaat
     6721 ataaaaatat ttgatttaaa taaaaatatg attgaaaaag agatattgat tcaataatag
     6781 taactagggg gaagtaatat gagaagagca agtggaataa taatgcatat tgcttcctta
     6841 cctggaaagt atggaatagg tacttttggt aaggaagcat ttgagtttgt agatttttta
     6901 aagaaagctg gacaaggata ttggcaaata ttgcccttag gtcctacaag ttatggggat
     6961 tcaccatatc aatcattttc agcctttgca ggaaatccat attttataga ttttgatatt
     7021 ttaaataaag aaggattact taataaaaaa gattaccaag gaattaattt tggaaatgac
     7081 ccagaaaaaa tagattatgc tttattattt gacaagaaga tgagagtgtt aagaatagca
     7141 tatgaaaaat ctttagataa aaacaaagaa gaaattgaaa agtttagaga agaaaataaa
     7201 ctttggcttg aagattatgc tttatatatg gcaatcaaaa atgaaaatga attagtaagt
     7261 tggcaagaat gggatgaaaa attaagatta agagataaaa agatcttaga agaatataaa
     7321 gttaaattag aaaaagaaat aaactactgg gtattcttac aatataattt ctttaagcaa
     7381 tggaatgaat taaaagagta tgcaaatagt tttggaatta agataattgg agatatgcct
     7441 atatatgttg cagaggatag tgcagatgtt tgggcaaatc caaaagcatt tttattagat
     7501 gaaaataata ttcctaaaaa ggttgctgga tgtccaccag atgctttttc agaaacaggt
     7561 caattatggg gaaatcctat atatgattgg aattacatgg atgacacagg gtattcttgg
     7621 tggattgata gagtaagaga aagctttaag ctttacgaca tattaagaat agatcacttt
     7681 agaggatttg aagcttattg gcaaatacca tatggagatg aaactgctgt aaatggtgag
     7741 tgggttaaag gccctggaat aaaattattt aatgcaatta aggaagagtt aggagaggtc
     7801 aatgtaatag cagaagacct tggttattta actcaagagg ttatagattt tagaaatgaa
     7861 actggattcc caggaatgaa ggttttacaa tttgcctttg attctaggga agaaagtgat
     7921 tatcttccac ataattatcc agttaactca atagcttata caggtactca tgataatgat
     7981 acatttagag gttggtttga agttacagga aatagagaag atgtggaata ttctaaaaaa
     8041 tatttaaaac ttactgaaga ggaagggtat aactggggat ttatcagagg agtttggagc
     8101 agtgtatcgc atacagcgat agctctaatg caagatttct taaacttagg aaatgaggca
     8161 agaataaact atccatctac tcttggtggc aattggcaat ggagagttaa agatgatgct
     8221 ctaactgatg aattagcaga gaagatatat gatataacaa aattatatgg aagggtgaat
     8281 attaatgaat aaagaacaaa taaagagagg aattgaaaga tatctaaaag taaagtatgg
     8341 taaaagttca attaaagatg ctaaggactt tgaaattttc aatgcattat cactaactct
     8401 tttagaggga attgttgacg attggaatca tacagaagag ctttatgaga aagaaaagaa
     8461 tgcatattat ttttcagctg aatatttaat gggtagagct ttaggaaata acttaataag
     8521 tttaggactt tataatgaag taaaagaagt tttagatgaa cttggctttg atttaaatag
     8581 aattgaggaa atagaagagg atgctggttt aggaaatggt ggattaggta gattagcagc
     8641 ttgttttatg gattcaggag cttcattaga agtaccttta aaaggttatg gaattagata
     8701 taataatggt ttattctctc aatattttga agatggattc caaaaagaag atgtggatag
     8761 ttggctaaaa tatggagaac cttggagcat aagaaaagag gatgaatttg taactgttga
     8821 ttttgaagat atgacagtta aggcaattcc atatgatact ccaattatag gatacgctag
     8881 taaaaatata aatactttaa gactatggaa gtgtgagcct gtaaaagaat ttgattttaa
     8941 attattcaat gaacaaaaat atgatgaggc attagagctt aaaaatagag cagaagatat
     9001 atcaagagtt ttatatccaa atgactcaaa tagagacggt aaattattaa gattaagaca
     9061 acaatatttc ttagtaagtg cttcattaaa agatataata agaaagcata aagaggtttt
     9121 tggaaacgtt actgaagatt ttgcaaagat gcatgctgtt caattaaatg atactcatcc
     9181 agttttagca attccagagc ttataagact tcttgtagat gaagaaggat ttacttttga
     9241 agaagcttac aatgtagctt caaaaacttt tgcctataca aaccatacta tattagcaga
     9301 agctttagaa aaatgggatg ttgacttaat agaggaatta ttcccaagaa tattagaaat
     9361 aattgaaaag atagatgcag agttcatagt tagccttgaa gaaaaaggat actcagagga
     9421 agagatagaa gagtttagaa tagttaacga tggaaaagtt agaatggcta acttagcaat
     9481 acatgtagga tttgctatta atggtgttgc taaattacac acagatatat taaaaaatat
     9541 tgagcttaaa aattggtatg aattatatcc agagaagttc caaaacaaaa caaatggaat
     9601 aactccaaga agatggttaa gactatgtaa ccaagagcta tcagctttaa tcactgaact
     9661 tttaggaagt gaagattggg ttaaaaatct agacttatta aaaggattag aaaaatataa
     9721 agatgatgaa gaagtcttaa agagattcat ggatattaag cacactaaaa aagagcaatt
     9781 agctgcatat attaaagaac atgaaggtgt tgagttagat ccggattcaa tatttgatat
     9841 acaaattaag agattacatg agtataagag acaattatta aatactctat atatcttaga
     9901 tttatattat agattaaaag aaaatccaga tatggatata ccaaaggtaa catttatata
     9961 tggagcaaag gctttcccag gatataagag agctaaatca atagttaaat taacaaatga
    10021 agttgctaaa cttataaata atgatgaatc tataaaagga aaaataaagg ttgtatttgt
    10081 agaaaactac agagtttctt atgcagaaaa attattccca gcagcagatg tatcaaaaca
    10141 aatatcaaca gctggtaaag aggcatcagg aacaggaaac atgaaattca tgttaaatgg
    10201 tgcaccaact ttcggaacta atgatggagc taatgtagaa attgttagag aaagtggagt
    10261 agataataac tttatctttg gattagaagt ggaagagata gaagaattaa aaggaaaata
    10321 taatccagtt gattactatg aatcagatgc agatttaaag agagttttag attcattagt
    10381 agatggtact tttgatgatg gaggtaatgg tgactttgaa gatctttata attcattatt
    10441 agaagaagga gatcaatact tcttattagc agattttaaa gcatttaaag atactgaaga
    10501 taaggtattt gaagcttata aagataaatt aggatgggct aaaaaggcct ttgaaaatac
    10561 ttgtaatgca ggtatgttct caagtgacag aacaatacaa gaatattgtg atgacatctg
    10621 gggaattaaa aaatgtacca ataaataatg gggaaaataa ctttataaaa acgtaataat
    10681 aaaacttaca aacatattca gaaaattgct agatattttc ttcatattgt ttgtaagttt
    10741 tatgttatat ataactagga aatataagta atcataggaa tagctttgtt aaaacttgaa
    10801 aacttagtat aaatccaaat tatatttgga tgataggaat ttatgttttt attaaagtat
    10861 aaatttaaag tttggttttg agttttgtgc aaggcatttt ctaaatctat ataaagaatt
    10921 aaaattcgtg aaatttatat ttaaatagtt aaaagggtac tttagatatt gaattaaggg
    10981 gtgtcaattt atgaagtttg gaaaaaaaga ttggactagt tatgatagag ggatagaaaa
    11041 agagtggatg cttactaatg gtataagtgg attttgtgga ggaactgcta taggagcaaa
    11101 ctgtagaaaa tatcaagggt tattagtagc gtgtactaat tctccagaag agagatatat
    11161 gattctttca aatttaaatg aggaaataaa tattgatgga aagcttcacc agttgagttc
    11221 ttgtaaatat acagataaaa tagtggatgg atttaaaaac cttcaaggat tttattatga
    11281 tggactacct cattttagat tttttgtaga tggagtagtt ataaataaaa agattgctat
    11341 ggaatatgga aagaacactg tagtaataga atatgacata ttaaatggaa gtaaagaatc
    11401 aaaaataaat attaaaccac tatttacttt taggaatcca ggaatatgta gtggagaaga
    11461 aaatttgaaa ttttcaaaaa ctgtggataa aaataaaatt caacttactc cacagttaaa
    11521 taaggacata aatataagat tttttaccta tggtggaaaa atattagata aaactgataa
    11581 aattgttgat aaaatttatt atgatgtgga taaaagtaca ggtgataagt ttgtggataa
    11641 atattacatt ccaggaactg ttgaaataaa tttaaatcct aatgaaagaa aaaaggttta
    11701 tttactttgt actatagaaa atgaagagaa ttttgactgt gaaaagataa tagaaaatga
    11761 agagaaaaga ataaataatc ttaaaaatac cttcgctgat tcaagaattt tagctaaata
    11821 tcttccaatt gcaggggatc aatttatagt aaatagagag tctataaagg gaaaaacaat
    11881 tttagctggc tacccttggt ttttagattg gggaagagat gctatgattg ctattaatgg
    11941 attaacttta tcaactggaa gattagaaga tgctaaagat attataagaa gttttagcct
    12001 ttatgagaaa gatggattaa taccaaatat gttccctgga aaaggacaag agccacttta
    12061 taatacagta gatgcttcct tatggtttat aaatgcagtt tataattatt tgctatatgc
    12121 taatagttac gaagctttag agtttgttga gaaagaagtt tataaaacta taaaaaatat
    12181 aataaaggct tataaagaag gtactaagtt ctcaataaaa atggatgaag aggattattt
    12241 aataaatgct ggttcaggat tagaccaagt aacctggatg gatgttagag taaatggcat
    12301 agtggttact ccaagacatg gtaaaccagt tgaaataaat gctttatggt ataatgcctt
    12361 aaaaatagca gccttattaa aaaataagtt tgaaaaagaa gaaaagaatg agtatgaaga
    12421 attagctaaa aaggttaaag attcatttac taagactttt tggaatgagg atagaaagta
    12481 tctttacgat gttgtaaata atcatgagaa ggatgatagt ttaagaccta atcagatttg
    12541 ggcagtatca ttacctttta caatgttaga tagagaaaaa gaaaagcata ttgttcaaaa
    12601 ggtttttgaa gaattataca ctccttatgg attaagaagt ttaagtagaa acaataagga
    12661 ctatcatgga atttatatag gaaagctttt tgatagagat atggcttatc atcaaggaac
    12721 aacatgggca tttcctcttg gaggattttt cacagcttac tgtaaggtaa atgattattc
    12781 taaagaagct gtagatttaa ttgattcttt aatgagagat atggaagatc atattaaaga
    12841 tcaatgctta ggaagtattg ctgaaatttt cgatggtgat aatccttata aagctagagg
    12901 atgctatgct caagcttgga gtgttggaga aatgcttaga gtttattatg aggatattct
    12961 aggaaactat aaaaaattaa aaaaagtcca caaagatata tttatataaa tgttttgcta
    13021 gagattaatt tagtgatctc tagctttttt aatttttgta attattaagt ttgtaatttt
    13081 aggatataat taaaggaaga acttagcata aaggggcgat ttacatgaaa acatcattga
    13141 atatagaaaa taaaattaat atgataactg aaatgagagg agattctgta tttcaggttt
    13201 tagagtatga tagtttaaag ggcggcaact ccttagaact tgcagttaaa ctaaatcaaa
    13261 tgaaggaagc taatataaag cttagacaag taagaattat tttagaagat agctctgtaa
    13321 atatagaaaa aggagcttta agctatatga gaggtccaat atctatgaaa aataagattg
    13381 gtggaccaat aggtttaggt aaaaaacttt tttctagtaa agttacaggt gagagtacat
    13441 ttaaacctaa atatgaagga acaggggata tatttttaga accttccttt gggcattatg
    13501 ccttaattga attagaagat gatgaaataa tagttgatga tggattattt tacgcttgtg
    13561 aaagtagtgt agaaataact gctcatatga ataaatcttt atcctctatg gttttaggaa
    13621 atgagggagt atttcaaact aagctttcag gaaatggaat agcggtttta gagattccag
    13681 taccagaaga agagatattt aaatgcaaaa taaataatga tgtgttaaag gttgatggta
    13741 attttgccat attaagaact ggagatattg actttacagt tgaaaaatca tcaaaatcac
    13801 tagtatctac agctactgga ggagaaggtt ttttaaatgt atttaggggg acaggtgaag
    13861 tgtggctaat accaactaaa tctgtttatg aaaagcttga gaagggatgg aattcattaa
    13921 aaccattaac agatccaaag ggtagctcta atactaagat ataatttata aagattaaat
    13981 tagttttatc ataagtatta ttttatttat tattataaag agatataatt tatttaataa
    14041 ataaaaatat ttaattacca taggtttgta ttaatataaa tgtagtttaa ggagaagatt
    14101 atatgaacgg agtacaatct aaactaaaag gcaaggattt agatttttta tttgaggcta
    14161 tattaaggtt aaaagatata gatgaatgtt ataatttttt tgaagatata tgcactattt
    14221 cagaattaaa agcattagca caaagattac atgttgcaaa aatgcttaag gaaaaaaaaa
    14281 catatactga aattgcagag gagactaagg cttcaacagc tactataagt agagtaaata
    14341 gatgtttaaa ctatggagca aatggctata acaatatatt aaatagatta aatgagaaat
    14401 aattagaaga ataattattt taaaaagagg catactatta acattaatat ttaaggagga
    14461 atatagtatg ccgaatttaa gctgtggtgt agataactgt gcatataatc aaaatgatat
    14521 gtgttcatta aattcaataa aagtaaatgg ttcatttgct aatcattcag aagctacttg
    14581 ttgtggaagc tttgttgaaa aaggcggatt tactaatgct gctgttgata tgggccctgc
    14641 agagccagca actagcgttg catgtgaagc tgagaaatgc tgctacaata aaaactgcaa
    14701 atgtcatgca gaaagcatag atatttcagg tgattatgca caacaccaag acaatacaga
    14761 atgttcaact tttagacaag aataataaaa tattaatttt actaatttag aaggaagact
    14821 atatgattta gtcttctttt taaataaatt tatttatttt attggacaag cttataattc
    14881 tacatattat ttatatagtt tttagtttaa atataactaa tgaaaattct aattatttat
    14941 aagaggtgga aagtttgaat atattattag ttgatgatga agaaaaaatc attgaggtat
    15001 tagaagctta tttgaaaaaa gaaggtttca gtgttttttc ttgtagtaat ggagaagagg
    15061 ctttaaaaat atttgataac aataaaatag atttgattct tttagatctt atgcttccag
    15121 atttaagtgg ggaagaggtt tgtaaggtta ttagagcaaa gagttgtgtt ccaataataa
    15181 tgataacagc taagactgag gaagaggatt tattagaagg ctttgatatt ggagctgatg
    15241 attatgttac aaaacctttt agtgtgaaac aactgattgc tagaataaaa gctattatta
    15301 gaagaagcac accatgtcct aatgagggaa gtgatatttt agtatttaat aatggagagt
    15361 taaaaattaa tgtagataca agagaagtat gggttagaga tgaattaata actttaacat
    15421 caacagagtt taatatactt ctttgcctaa gtagctatcc aaagaaaata tttactagag
    15481 atgaaataat agagctagta ttaggggata aaagtgatag ttttgatagg gttatagatt
    15541 ctcacataaa aaatttaaga ggtaaaatag aagaaaacac tagaaaacct aagttcatag
    15601 taactgtata tggggtggga tataaatttg aaggtaagaa aatataaaag tttaaatttt
    15661 aaggttatag ttccatctat agttatcata ataattgtaa ctttacttac tgtggtcata
    15721 tctaaatttt attttgataa aaaatttggt gactatatta tgattaaaaa tcagaataca
    15781 gtacaaaaca ttttgatgga gctaagtgaa caatattctg ataataagtg ggattacaag
    15841 ggtatggaga aaataagcta taatgcatta gataaaggga ttatagttgc tttatatgat
    15901 aaagaagata aggaaataat gaatatagaa aaaaattcta aagataaatg caatagaatt
    15961 atgaatttta taaaaagttc tatggaaggg aaatatggct ctacaacaag tcaatttgaa
    16021 cctgtgtatt atcctttaat aaagagtgga gaaaaaattg gagaggttag aattaaattt
    16081 tatggcccta tattttatat gcagaatgaa ttagtttttc tggatatagt aaataagata
    16141 atattaggta ttggagtttt actgattcta gcatctacag taattgggtt tataatttca
    16201 aggtcaataa caagacctat aaataagcta atgacaaagg ctaagtatat ttctaaaggg
    16261 gaatatgaca agaagattga gataaataca gatattttag agataaatga tttgataaat
    16321 tccataaata atctttcaca gagtataaaa gagcaagaga acataagaaa aagacttact
    16381 ggggacattt ctcatgaatt aaaaactcct ttaacaaata tacagtctca cttagaggcc
    16441 atgatagatg gaatatggga acctacagag gaaaggttac taagtgttaa ggaagaggca
    16501 gaaagactat cgtccttagt aagtgatatg caaaagttaa ataagtatga tgagtcttct
    16561 attaagctta aaaaggataa tgtgaatata agtgatataa tttgctttgt aatatttcaa
    16621 ttttctaatt tagcaaagag taaaaatata aagatagaat atgaaaaaaa gaatattaat
    16681 ttatattgtg ataaggataa aataacacag gctttagtaa atattctttc aaatgctatt
    16741 aggtactcaa atgagggaag tactattttt atagaagaaa aattaaaaga taataaggtt
    16801 ataatatcca tagaagatca aggtataggt atttcagaag aggatttaaa atatgtcttt
    16861 gaaagatttt atagggcaga taagtctagg actagagcta ctggaggaac aggaatagga
    16921 ctaactatag ttaaatccat agtatcttct catggaggag aggttaagtt agaaagtaaa
    16981 ctaggtgaag gaagtaagtt tactataata ttacctaaga aagatatatg attattttat
    17041 ggaaaagtaa tcttattttt agggctatat aaataagtaa ataaattaga aactacataa
    17101 ttttagttta acagtttgaa ttatattatt atgaaaattg aaattgtata gtttagtaaa
    17161 acaatatacc ccttaagtag attttaaaaa ggtgtttttc ttaaatctac ttaagtggta
    17221 ttttaaatat ttataagtta acaacatata taataatata caaaatatat tttcctatgt
    17281 cataacctat atttttattt taaattgttt agttcatttc ttatttcatg catttcattt
    17341 tgaacttctt taactttagc tttattgctt tcaattctac ctttgatatc ttcaactatt
    17401 tcttggtatt tttcaatagt ttctaaagag ttgtttccag gtctaacatt aaataatctg
    17461 ttagtaaagt cagaaagtct ttcttcatct tggctaatat atttttttat ctttttaaca
    17521 aactcatctt tatttttaag taaagagttt aagaaatctt tttgtttatt agctctagca
    17581 gcatttatct tatcaaaata ttcttgtcta gcagcgtaga aaccatctct tatttcttta
    17641 aactcgttta taagtttttc attttcttct tttccgcaga atcctatttt aaagaattca
    17701 tcacttattt ctttaacctt agcagaagct tctttccaac tatcattttc atatagtttc
    17761 tttaattctt ctataagagc ttcccttttt tctatattaa ctttattctc agcttttaga
    17821 gcatcaaaaa attcttgtct cttagcaaag aaagcatcct tagcttcttt atatcctaga
    17881 gaaatttctt cgtttccttc ttttccagag aaacctatac ctttaaattc attgcaaaga
    17941 gcattgaatt tttcagtagc ttctttccag ttttcattat taactaaagc tttaagttct
    18001 gagataattt ctctttttct atcagcttta tttccgaatt cttctcttaa gccttccata
    18061 gcagcttttc tagtttcttg agctgcattt ctagcctttt taaatctatc atataattca
    18121 gtgtttaaat ctttacctgc aaagcctata gcatagaatt cttcagataa agatttagtt
    18181 aacatatcag aagcttttat atcacttgta tatactaagg cttcaagtct ttttataaca
    18241 tcttctttct tttcagcatt ttctttgaaa ccttcatttg ctttttcaaa gaattctttt
    18301 ctagcagtgt agaattcatt tttagcttct cttaattcat tttttagggt attctcagtt
    18361 tcttggtcat agtaataaat attattaaac tcttcaataa gagcgttgaa ttctttatta
    18421 gcttctttcc aattttgagc actttttgct aagcttctta ttttctcgat tacttctttt
    18481 tttctcttac aagaattttc ctcttcagca gctaaagctt tagcaaaatc ttcagtttct
    18541 ataggatttt tattttcatc tatgtaatta gattcattag ttgtcatgta aattatcccc
    18601 tttcttttat atcactcatt aatattttaa ggcgtaatta gacttatgtc cataattaaa
    18661 atgttttcaa taatatttat gtaggatata tagaaatata aagtttatgg ctctttaaat
    18721 agacataaat ttattacctt tatagtttca agtatatgcg aatcttgatg atatattata
    18781 ttaatataaa taaatctatg taataattag tgaaaataat gttaactaaa acaagctaaa
    18841 aataatattt tagtaataaa tatattttat tcatgtggta acataaaaaa tgactatata
    18901 atattaaaaa attttaattt ttaataacta atatttgaca aatattatta catacagtat
    18961 tctttgaaag tgtaaaaagt attaattaag tagcaaaatt ataggtattt taattaaata
    19021 taaatataat ataaaattat tagaggggtg acataaattg tcaagattat ttggaacaga
    19081 tggagttaga ggaatagcaa atactgaatt aacagcagaa ttagcatata acttaggtag
    19141 agctggagct tatgttttaa cagagggaac tcataaacct aagatattag tagcaaaaga
    19201 tactagaata tcaggagata tgctagaagc agctttagtt gcaggcatat tatcagttgg
    19261 agcagaggca ttatgcttag gagtagttcc aacaccagcg gtagctcact taacaagagc
    19321 atatggagca gatgctggtg ttatgatatc agcttcacac aatccagtgg aatataatgg
    19381 aataaaattc tttgatgata agggatataa attatcagat gatttagaag atgaaataca
    19441 aagagttata gaaagtggct ttgaaaatgt accttcacca acaggagcta atttaggaag
    19501 agaaataata gaaaaagctg ctttagaaga ttatataagc tttgctaaag atacaatagg
    19561 aatatcttta gaaggattaa gagtagcttt agattgtgca aatggtgcat ctcacgaagc
    19621 tgctgttaga gctttcagag aattaggagc ggaaatattt gtaataaatg ataatccaga
    19681 tggaactaat ataaatgaaa attgtggatc aactcatcca gaagaattaa tggagtatgt
    19741 tgttaagaaa aaatgtcata tgggatttgc ttttgatgga gatgcagata gatgcttagc
    19801 tgttgatgag caaggaaact tagttgatgg agatttcatc ttaacaatat gtgctaaata
    19861 cttaaaagaa ttaggtagat taaaagatga tactttagtt gtaactgtaa tgagtaactt
    19921 aggattaatg atagcttgta agaatgaaaa aataaacact gcagttacaa aagttggaga
    19981 tagatatgta ttagaagaaa tgttagctaa aggatattct ttaggtggag aacaatcagg
    20041 tcatatcata ttcttagatc ataactcaac tggagatgga cttgttacag cacttcaagt
    20101 agctagcata gttaagagaa ctggaaaatc tttatttgaa ttaaaaaatg taatgaaggt
    20161 attaccacaa gttttagtta atgcaaaagt acctaataac atgaaaaata tttatgaaga
    20221 agatgaagaa ataatagctg aaatcaagaa aatggaagct gctttagatg gttgtggaag
    20281 agtattaata agaccatcag gtacagaacc attagtaaga gttatgcttg aaggtgaaaa
    20341 ccaagctgaa atagatgaaa tggctcataa cttagcaaac atgatagaag caaaatgtaa
    20401 ttaagatatt atattaaact ataaaaatat tatataaaac cataaaatta taaaagctat
    20461 aaataacatg aatagtttta aataaaatta tcatgtattt atagcttttt atgatttaaa
    20521 atatttttat ttatttgatt atgaaaagtt atattaattc tcttcaattg gtccatattt
    20581 atctgaatca agtctcatta aggcaagggt aagttcctcc acatgttctt tttcctcctt
    20641 agttatatct cttatcattt taacaaggca tttatcttct attttattta ttaaatcatc
    20701 atagagtaat atggcctcgt attctccttt aatatcatct cgaattttat ttaaaatact
    20761 ataagcagtt aattttttat tttcgtgata tgttttcttt aaaggtttat cttttatttc
    20821 tacatgatca actgattcta catagacttc aaattcctct ttatcatatc ttctgagagc
    20881 ttctaaaaat tgaccatagt gtcttttttc ctcttccatt atgtgatgta ataattcttt
    20941 tatctccact aaagaaattt catctatatg tttgctataa ccgtttatag caactagttc
    21001 tgagattaaa gcttctctta aaaatccttt tatatccaag gcttttcctt gaggttgttt
    21061 ttctgtagta tagctcataa aaaatctcct aaattatttt tatagtattt ataatatgac
    21121 ttttcccaag aaaaggaaca gttaaaaata aatatttact tattttaata ttttttataa
    21181 gaaattaaag tataaaaatt aattactata aataagttag cttttttact gagaaatttt
    21241 aaacaatgga aagcgtctat acactaatta tactttgaag ttaaaatgaa agaaataaat
    21301 ataaaaattt cttttataat gtggaattta aaaatttaat tttaaaatta aatactatag
    21361 atgtctatac aactatacat taatatatta aataaaagaa atatagaggt atatacagat
    21421 aattaaaaat atttcttaaa aataaatgaa aataactaaa aatgaactaa aataagtttt
    21481 aaaagcttga atttttttaa aaaagtgtta gaataagttt gtagcttttt agaaatatga
    21541 tgcatagaat atattaactt tcaaaataat agattaaagc gccagaactt aaaattttat
    21601 tgatagacta ataagtaatt taactctagt agcttaaaat atattagaat ttttaagatt
    21661 ttaagttgac gaggttgggg agtatcgaat tttcggcgga tgccccacgg taaagcacta
    21721 ccgtaaaaaa ttggttaaag attaaaagcg attttaagga caaagccgat tgggtgttta
    21781 aaaccttcta tgcagtaaga agttatatag agcttatttt gaaagttaga atataatttt
    21841 acaaaaggga aggaagaaaa taatatgtgc ggaatagttg gatacgtagg acagaaaaag
    21901 gcaacagata tattagttga aggattatca aaattagagt atagaggata tgactcagct
    21961 ggagttgcag ttttagaaga taataagata aaggctgaaa agcataaagg aagattagca
    22021 aacctagaag gaatgttaaa tgaaaatcca atagagggtg gaatcggaat aggacatact
    22081 agatgggcta cacatggaga accatcagat gttaactcac atcctcattt aaataataaa
    22141 gaaacaatag ctgtagttca caatggaatc attgaaaact acaatgaatt aagaaactgg
    22201 ttaatggaaa aaggatatga attcaaatca gaaactgata cagaagttat tccaaactta
    22261 gtagattttt actataaagg tgatttatta gatgcagtta tggaagctac taaacacatg
    22321 gaaggttcat atgctattgg agttatatgt aatgatgagc cagaaaaatt agtagctgta
    22381 agaaaagata gtccattaat agttggatta ggagaaaaag aatatttcat agcttctgat
    22441 atcccagcag ttttaaatta tacaagagaa gtttatcttt tagaagataa agagtttgta
    22501 gtattaacaa atgatggagt tactcttttt gatgaagaaa aaaatccagt ggaaaaagaa
    22561 gtttatcata taacatggaa tgttgatgct gctgaaaaag gtggatatga agatttcatg
    22621 ttaaaagaaa ttaacgagca accaaaagct ataaaagata caatgacttc aagaataatg
    22681 gaaggaaaag aagtaacttt agatgatata tcaataacta aagaatattt agataatgtt
    22741 gatagagtat atatagttgc ttgtggaaca gcttaccatg ctggagtaat tggaaaatac
    22801 gctatagaaa aattagttag aataccagta gaagttgata tagcttcaga atttagatat
    22861 agagatgctg ttattacaga taaaacttta ataatagtat taagccaatc aggagaaact
    22921 gcagatactc tagcagtttt aagagatgga caagcaaaag gtgcaagagt gttagccgta
    22981 actaacgtag taggaagctc agtttcaaga gaagctaacg atgtattata tacatgggct
    23041 ggtccagaga tagctgttgc atcaacaaaa gcatatgtaa ctcaattaat tgctatgtac
    23101 acattagcat tacactttgc tgaattaaaa ggaagcaaat cagtagaaga aattgaggaa
    23161 atcaaaaaag caatgcttga attaccagaa aaagttgaag aaatccttaa aaatacagat
    23221 ttaataaaag agtttgcagt taaagcgtct acagaaaaag atttatactt cttaggaaga
    23281 ggtatggatt atggagttgc aatggaagga tcattaaaac ttaaagagat ctcttacatt
    23341 cactcagagg cttatgcagg tggagaactt aaacatggac caatagctct tatagaaaaa
    23401 gatattccag taatatcatt attaactcaa agagagctta tggataaaat gataagtaat
    23461 gttcaagaag ttgttacaag aggagctaac gttttaggtg tttgctttaa aggtgacatg
    23521 gaagaaagta agagaaaaat gtttgaagga ttaatagaaa taccagaaac attaagctta
    23581 ttatcaccag ttttaagtgt tgtaccatta caattattct catactatgt ggctaaagct
    23641 aaaggatttg atgttgataa accacgtaac ttagctaaat cagtaactgt tgaataaaaa
    23701 ggggattttg aatatgaata atgagagttt attaaaatta ttagcagagt ataaggaaac
    23761 taaaaaatgc cttgaaacag gattaaactg gctagaagag aaagactatg ctaaaggtaa
    23821 gttagatata gtaaatgtaa taataagaga tttagaagca gcaattggtg ctgaaagaat
    23881 ttaatataaa attagcttaa aattttaacc ttatttatat gtgttagggg tgtttaaatc
    23941 attaaatata gtacttaaaa tgctataaag tgattttaaa cactccttat tttagtgaaa
    24001 atatattgaa aaatactttt tattgaagta taatactttc gctaaaacct cataattctg
    24061 aggaataagc taatattaat taatgaggta tttatagtga aaaattttca agaaaataat
    24121 attttagaag ataaaaaagt atgtataaat gaaaatgaag aggcttggaa taaagagaca
    24181 tataatgctt gggtaaagag atttggaact gctaaggaag cagcggaaaa aattaaatct
    24241 atgcctgaaa aaaaattaaa tgttttgtta gataaatttg gagatgttaa aggaaagaag
    24301 atatttaatc ttatgggctc taatggaaat aaggctgttg cattagcttt attaggggca
    24361 gatgttactg tagtggattt ctcagaagga aatagaagat atgctttaga tttagctgag
    24421 gcttgcggag taaagattaa tttcattcta agtgatgttt taaaaatgcc taaagaagtt
    24481 atgagtggag attatgacat agtatttgca gagatgggaa tacttcatta tttctcagat
    24541 ttatctcctt ttatgaatgt tatacatagt ttattaaagg atggaggaaa agctgtatta
    24601 agagattttc atcctgtatc aactaagtta ataacttcaa gaggatctac agcaaaggta
    24661 agaaagcata aagttacagg agattacttt gatacttcat tagaagagaa agaagttgct
    24721 tactcaaaat acttagaaga aggaaaagta gaaaaagtat ttttacgtaa gtggaattta
    24781 ggagaaatag taactgccgt tgccaatact gggcttaaga tagaaagttt aacagaggag
    24841 cctaatttat caagtgatgt ctttgataaa ggaattccaa agacttttac aatagtagct
    24901 agaaaataat ttaatagact tggtcgtgta tcaaaataga tttttataaa acttacaaac
    24961 agtatgaaga agtaattctc ggaatatgtt tgtaagtttt attattatat tttaatacag
    25021 catattattt tttattatga tgtatttata cgaaaagaat attaagtgta tttatatagt
    25081 attgcaatac agaatatggt aaaattatta aaagtctatg aggaggaatt tcatatggat
    25141 aagggagagt taagaaaaga aattaagcta aaaagagaaa atatagatag aaatactaag
    25201 ttaatggctg atgaaaaaat aagaaaaact cttttggaaa gtgagatata taagaatagt
    25261 aaggttgtat ttatatatat aaatatggac tctgaagtaa atacagttga gataataaaa
    25321 gaattattag cttcagataa aaaggttgca gttcctaaag ttattcctgt tagtttaaaa
    25381 gaaagacaaa tgaaggcttt gaaaatagat tccctgcttc aattaaatga aagtggtgcc
    25441 tttggaatat tagaaccaag cattgaatgt aaagatataa gtgaggaagt tgatttaatt
    25501 ataatacctg gtttagcctt tgatttaaag ggaaatagat taggctatgg aggaggattt
    25561 tacgatagat ttttaagtaa ataccctaat agtaaaagag ttgctttatg ttatgatttt
    25621 caagtttttg atgaaattcc aaatgaattc tttgatgaga aggttgattt aataataagt
    25681 gaagagaaaa ttattagttt aaaaaaatca taatagtaaa ataatatttt tgataatttt
    25741 actttattga taataaattt tacttatgtc agaattatat aaaatataat tgtgaggtgg
    25801 ggtaaacaat gaaatttgaa gattttaaat atgaaagacc taatatagaa aaagatagag
    25861 aagatattag atcttttata aaagagttag aagaagtaaa ggactttaat ggggctaaag
    25921 aagtaatagg caaaataaat aagataagaa ataatacatc aactatgatg gccttatcag
    25981 aagttagaca cacaataaat actgaggatg aattctataa tgaagaaagt gatttctggg
    26041 atgaaaattc tcctcttatt gaagaagtta ataatgaatt ctataaagca ttattaagtt
    26101 caccatttaa ggatcaaata ggtgattatt atggtgaaac tataataaaa gaggcagagt
    26161 attctcaaaa aagtttttca aaagaagtaa tagaagatat gcaacttgaa aataaattag
    26221 gtagtcaata tcaaaaactt attgcttcag ctaaaattga atttgatgga gaggaaagaa
    26281 ccttagctca attagttcct tttgtaacat cagaggatag agaagttaga aaaagagcat
    26341 cagaagctaa atataacttc tttgtgaagc atgaagatga aatagatgat atctttgata
    26401 aacttgtaaa ggtaagaaca aaaatagcta aaaagcttgg ttttaataac tttgtggaat
    26461 taggctatgt aagaatgaga agatatgact ataataagga aatggttgaa aacttcagaa
    26521 aacaagttga agagtttata gttcctatag atagtaaatt gtatgaaaga caagctaaga
    26581 gattagggct tgaaacttta aaatattatg atgaaaaatt tgaattctta agtgggaatc
    26641 caactccaaa gggagattca aagtggatag ttgaaaatgc aaaggtaatg tattcagagc
    26701 tttcaaagga aaccaaagaa ttctttgact ttatggttga gaatgattta atggacttag
    26761 ttgctaagaa aggaaaggca gctggaggat attgtactaa tttccctaac tataaagctc
    26821 catttatatt ctcaaacttt aatggaacag ctggggatgt agatgtatta actcacgagg
    26881 ctggccatgc gttccaaaac tttagaagtt cttggataga aatgcaagag tgtcaatggc
    26941 caactatgga aagctgtgaa atacactcaa tgagtatgga attctttact tggccttgga
    27001 tgtatttatt ctttaaagaa gatacagata aatataaatt ctatcactta ggaaatgcca
    27061 ttaaatttat tccttatgga gtaacagttg atgaattcca acactttgta tatgaaaatc
    27121 cagaatgtac accaaaggaa agaaaagatg catggagaag aatagagaaa aaatatcttc
    27181 cacataaaaa ctacgatgaa tgtgatttcc tagaaagagg tggttggtgg ttccaacaaa
    27241 accatatatt cttatcacca ttctattaca ttgattatac tttagctcaa atatgtgcac
    27301 ttcaattctg gaaaaaagat agagaaaatc atgaaaaagc atgggaagat tatttaaatc
    27361 tatgtaacat aggtgggact aaaacattcc taggcttact aaaatatgct aacttaaaat
    27421 caccctttga aaatggatgt gttgaaagta tagtaggagt agtggatgaa tacttagaaa
    27481 gcatagacga cagcaaattt tagtttttaa aacaaaattt attatttatt tagagtatga
    27541 ttaagtaagt ttattatgaa tatagattaa acttattaaa attaataata actataaaat
    27601 actattaatt ttattaagta tagatttgtt tgcttaatat ttcttttaaa ataaaaaact
    27661 tggagtattc cttatggatt tcataggaat acatccaagt tcttttttga ttataaatta
    27721 aaatgttatt aatataagct tattaaaaat ataaatagta tgaagaggat atatgtatga
    27781 actttggaat ttaagtaatc atggaaagaa atttgcgtta agaagattga atgttcgacg
    27841 cgaagctagt tttcaatctt tagtaaattt cttggaatga ttaccataaa ttcttagtga
    27901 aatacattaa tcctcggaat atatttatat ttttagttaa ttaggctttg tattttctta
    27961 gttttctctg ttagctattt caagaactat tctgtttacg aagtcagcta aaggcatagc
    28021 accttcttca ccgttctttc tgcttcttac agaaacttct ttgttttcag cttctttttc
    28081 tccaacaact aagatataag gagttctttc taatctagct tctcttattt tataaccgat
    28141 tttttcagct ctgtggtcag cttcaactct aactcctctt cttcttaatt cttcaacaac
    28201 ttcatttgca taatcagcat atttatctga taaaggaaga actttagctt gaactggagc
    28261 tatccaagtt ggaagagcac ctgcatattt ttctataagc attgctagag ttctttcata
    28321 acatccaatt gaacttctat gaatgatgta aggacgtttc ttttctccat ctttatcgat
    28381 gtagtacatt ccaaatcttt cagctaaagc gaagtctatt tggatagtga tgattgtatc
    28441 ttcttttcca tgaacattct tgaattggat atcaagtttt ggaccataga aagcagcttc
    28501 atcatcagct tcagtaaagt ctatatttaa gtggtttaat atttctctca ttatattttg
    28561 agtattttcc caagcttcag gatcattgat gtatttctca gtattgtttg gatcccattt
    28621 tgagaatctg taagtaatat catcagcaat tcctaaagtt tccataacat atttaactaa
    28681 gtcaacagtg tttttgaatt catcttctaa ttgctctgga gtacatacta tgtgtccatc
    28741 agcaagagtg aattgtctta ctcttataag accgtgcatt tctcctgaag attcatttct
    28801 aaatagagta gaagtttcag caaatcttac tggtaagtct ctataactgt gttgagttga
    28861 gttatatatt gcgtattgga atgggcaagt cattggtctt aaagcaaaca cttcactatc
    28921 tttttcctca tcacctaaaa cgaacatacc atctttatag tgatcccagt gtcctgaaat
    28981 tttgtaaagg tcacttttag ccattaaagg agttttagtt aaaacatatc ctcttctttg
    29041 ttcttcgtct tctatccatc tttgaagagt ttggattatt ctagctccct ttggcattaa
    29101 tagtggaaga ccttgaccta cattttcatc agtagtaaag attccaagct ctctacctaa
    29161 tttattatgg tctcttttct tagcttcttc taaagcttct aaataagcat ctaattctga
    29221 tttcttaggg aaagcagttc catatactct tgaaagcatt ttgttctttt cgtcaccttt
    29281 ccagtaagca ccagtagatc ttaataattt aactgctttt attggtttaa cagccattaa
    29341 gtgaggtcct gcacaaaggt caacaaaatc acctatttga tagaatgaaa ttacttcacc
    29401 ttctggaaga tcatttataa gttcaacttt gtaaggttca tccttcataa gttctaaagc
    29461 ttcgtttcta ggaagttcaa atctttttat ttcaggattt tcttttatta tttttttcat
    29521 ctcttcttct aatttgttta attggtcagc agagaatgat ttttcagtat caaaatcata
    29581 atagaaacca ttatctattg atggtcctat agctaattta tcttgaggga ataatctttt
    29641 aacggcagct gctaatacgt gagaagcagt gtggttaaaa gcatgttgac cttctttaga
    29701 atcaaaagta cagatttcta atgaagaatc ttcattaact ataaatctta agtcctcaac
    29761 ttttccgttt aatataccac aacaagcatt tctagctagt ccttggctta tgctttgagc
    29821 tatttcaaaa attgatagtc cagcttcaac ttcttttatt gaaccatctt taagtgtaat
    29881 ttttatcata ttaatttctc ctttcaaaat aaatttcttt taattttcat tcatataaaa
    29941 taaaaactct catccctctt ctaaatatag aaaagggacg agagtgtata ttcccgtggt
    30001 tccacccaaa ttgtacttta ataattaaaa ataaagtacc acttaaaatt aatcgtaacg
    30061 tgattatgac ggactggatt agagtcactc agaggtagtc ttcaactaat tccatttaaa
    30121 agtacttaca gctactgtac cttctctctg aaaatgtacc aaagtttact cgtctcttca
    30181 ttgtgttaat aaatttataa tatttacttt agattataag tttattattt ttgtatgtca
    30241 atggataaaa taaaaataaa ttctaattgg aatataagat attataaggg tttaggtgga
    30301 aataagttct caatattata aattttaatt caaaaaaatt actaagttaa ttaaagtttt
    30361 tagaaaataa tttgcaaaaa aatcttgaaa tagaatttga aaaatattat aataaaaaca
    30421 tagttttgca ggtgtggcgg aattggcaga cgcactagac ttaggatcta gcgcctacgg
    30481 cgtgggg
//
LOCUS       DAEVGI010000015        29043 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEVGI010000015 DAEVGI010000000
VERSION     DAEVGI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.015"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            211..1686
                     /gene="spoIVA"
                     /locus_tag="K8M77_000645"
     CDS             211..1686
                     /gene="spoIVA"
                     /locus_tag="K8M77_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBI7085162.1"
                     /translation="MEDFNIYKDIAERTQGDIYVGVVGPVRTGKSTFIKRFMDLMVIP
                     KIDNAYKKERAKDELPQSGSGKTIHTTEPKFVPNEAVEIALDDGIKFSVRMVDCVGYI
                     VKGANGYFDDGESKKVHTPWFDYEIPFEDAAEIGTRKVITDHSTIGLVVTTDGSITGI
                     DRDDYLDAEERVVAELKSIDKPFIIVLNSLDPRAEETLDLKQELEIRYGVPVQIMDVA
                     NMNENDINDLFTKVLKEFPVKEINIDMPKWIEKLEPSHWLKSNFIDIVKDMCKNISKI
                     RDVKDLLSTYGEDFLGVADISEMNLGDGTVRVKMTPKNGIFYKIISEMCDEELNDESD
                     LIALIKDLHKAKSEYDKVAEAINSVKETGYGLVAPQLSEMKFEKPDIDKQGSKYVVKL
                     KASAPSLHLIKADIQTEICPIMGTEKETQEVFKTLLEQFESDPEKLWQSNMFGKSLET
                     LVQEGLRSKLYKMPDDIQSKIQKTLQRIINEGEGNLICIIF"
     gene            2038..3021
                     /locus_tag="K8M77_000646"
     CDS             2038..3021
                     /locus_tag="K8M77_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBI7085163.1"
                     /translation="MKKIAVIFNGGTISMKVDDRIKAAVPSLTGEEIMAMVTGIESFA
                     TIESHSFSSLPSPHITPEIMLELSKFINSLVEREDIDGVVVTHGTDTLEETSYFVNLT
                     TKTSKPIVFTGAMRSGSELGYDGPSNLAASICTAASDEAKNRGVLVCFNGELNSASEV
                     TKANSMALNAFRTPNFGPIGIVDNNRVIFDRRVPQEDFIPLEVIDKKVAIIKCAAGMD
                     GDFIYHCIEKKYDGIIIEALGRGNVPPTMVDAIKDALDNGIVIVLTSRCFEGRVSDSY
                     GYLGGGKNLKELGVIFGESMPSQKARIKLLTLLGFNKDYRYIKHSFEKERY"
     gene            3278..4675
                     /locus_tag="K8M77_000647"
     CDS             3278..4675
                     /locus_tag="K8M77_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBI7085164.1"
                     /translation="MENKIHALSEEGNLRVLPENKSEYKREFLAGTTSFLAMAYIIAV
                     NPSILSAAGMPAGAIVTATCISAVIGCLIMGFYAKLPFGLAPGMGLNAFFTFSVVIGM
                     GISWEVALTAVFVEGIIFILLSLFKVREAVVDAIPINLKYAVTAGIGLFIAFIGFNGA
                     GVVIGNPDTMVAMGQVGPKMLIAMVGLCIIVILEKKKVKGSMLVGIVVSTLLAWGYAS
                     INTEAAASMGIYLPNGIFKFESIAPIAGKVNFSYLTSPQHVFNFITIVFTFLFVDFFD
                     TVGTLIGVASRANMLDKKGRVPNAGKALMTDAIATTAGALLGTSTVTVYVESATGVEE
                     GGRTGLTAITIGALFFVAMFFSPIFVSVPACATAPALIYVGYLMLTSVLKIDFSDITD
                     AVPAFLIIALMPLTYSIGDGLTIGVLTYVILNILHNIFAKNKEDKKELSMVMIVLAII
                     FVIKLCLPLITQMIG"
     gene            4895..5779
                     /locus_tag="K8M77_000648"
     CDS             4895..5779
                     /locus_tag="K8M77_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBI7085165.1"
                     /translation="MVKSMTGFGRATSEEGKERIFSLEMKSVNHRYLDMNIRMPRSMV
                     SLEEKIRNIISSKLSRGKVDIFINYKDYAKNQGVAVLNEDLAKSYVNSLEQLKSLFPN
                     MQDDLSLSLVARYPDVITIEEKSEDLEAIWEEINSLLNMAVENMISMRKVEGEKLASD
                     ILVKCSSIEEIVAFIEEKSEVIVASYKQKLEDRLKNLLGEVPVDENRVAMEVAVFADK
                     ASIDEEIIRLRSHINQLRKTLTLDEPIGRKLDFIVQEMNREANTIASKSTDLEITNKV
                     IDIKNIIEKIREQVQNIE"
     gene            5793..6062
                     /locus_tag="K8M77_000649"
     CDS             5793..6062
                     /locus_tag="K8M77_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HBI7085166.1"
                     /translation="MSIKLINIGFGNIVSANRLVAIVSPESAPIKRIIQEARDRGMLI
                     DATYGRRTRAVIITDSDHVILSAVQPETVAHRLSTKEEVVVEDDE"
     gene            6052..6702
                     /gene="gmk"
                     /locus_tag="K8M77_000650"
     CDS             6052..6702
                     /gene="gmk"
                     /locus_tag="K8M77_000650"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBI7085167.1"
                     /translation="MMNKIHKNNRGVLIVISGPSGAGKGTICKALLEKHDDIFISVSA
                     TTRNPRVGEVDGVNYHFLTKEEFKQRIAEDDFLEHAEVYGNYYGTPKSSVEKMLDEGK
                     NVILEIDIQGALKVKEKATDGVFIFILPPSMEELKQRIIKRGSETPESLMTRFKSAYK
                     EINYVSKYNYAVVNDNVEDAVKKIEAILLAEKCRVDRLKENLLESKEDEMHEQLYD"
     gene            6683..6895
                     /locus_tag="K8M77_000651"
     CDS             6683..6895
                     /locus_tag="K8M77_000651"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBI7085168.1"
                     /translation="MNNSMINPSIVDLLKRVEDRYSLVILSAKRARQIIDGAETFVDV
                     ESNKPLTIAINEIDEGFVNYKDTEEK"
     gene            6901..8088
                     /gene="coaBC"
                     /locus_tag="K8M77_000652"
     CDS             6901..8088
                     /gene="coaBC"
                     /locus_tag="K8M77_000652"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBI7085169.1"
                     /translation="MKDKKCVVVGVSGGVAVYKALDVISRLRKKDVEVHVIMTKSATE
                     FVTPLSFQSLSQNMVIIDMFAEPKAWEIQHISLAKKADLMLIVPATANIIGKVANGIA
                     DDMLSTTIMATKAPVIFCPAMNTNMYENPIVQRNINLLKEFGYEFIEPASGRLACGDE
                     GKGKLQDAEIIAEETLRRLHSTKDLLGKKVVVTAGPTMVPIDPVRILTNRSSGKMGYS
                     IAEEARDRGAEVVLISGPTSLIKPNGIKVIDIKTNEDMFNAIKNEFKDADIVIKSAAV
                     ADYKAKNYSNEKIKKTGDDLNLIFERDRDILKTLGDMKENQILVGFAAESSNLKENAK
                     GKLERKNLDYIVANDISNQETGFASDENKVTIISKSGEEVSLEKMSKREVAKNIFDII
                     KGR"
     gene            8127..10322
                     /gene="priA"
                     /locus_tag="K8M77_000653"
     CDS             8127..10322
                     /gene="priA"
                     /locus_tag="K8M77_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBI7085170.1"
                     /translation="MEKFAEVIVNSEAVTIDKPFTYKIKEDLVHNIKVGHRVLIPFGN
                     GNKKIEGFVLKILDNVENKSIRYKSIYNVCDNEPLLSEDSLKLVKFLREKYLCKYIDA
                     IRVMIPPKIMSGNKEKTKQVIVFKKYIEELSEPQKRALELIEEKSGGFTKAEWNKIFN
                     ISTYMINKLIELGCVSSEEVRVGRENLREFKPYPPKSLNEEQIRAYDTILESDKNIFL
                     LKGVTGSGKTEVYMNLVSYMLLFEKSSLILVPEIALTPQMIERFKGRFGKDVAVFHSR
                     LSDGERYDEWYRVKNDQVKVVIGARSAIFLPFNDLGLIVVDEEHESSYKSDMNPKYNT
                     IEVCEYLSVLKNCKVVLGSATPSVVSFYKALKGNYELIELKKRVNNLELPNVSIVDMR
                     EELKSNNKTMFSRDLYLKIKDRLEKKEQIILFLNRRGFSSFVSCRSCGFVFKCNNCDI
                     SMTYHNNGYLVCHYCGNTEKMPKLCPKCGSNYVKHFGVGTEKLEEAVHHHFKEAKVLR
                     MDVDTTRKKNSHEEIYNSFKSKEADILIGTQMIAKGLDFPDVTLVGVMAADMSLNLPD
                     YKSSEKTFQLITQVSGRAGRGDKKGDVIIQSYSPEHYALTCARDNNYEEFFKREINLR
                     KLMAYPPFDDLFLINLSAKGEIKVKIFAKKLEAYLRNILKEYDNIFIYEACPCSISKI
                     KNVYRWQILIKGKLDLEVCSKIKKGVYELSKDVYNEIKIGLDINPNSLM"
     gene            10336..10779
                     /gene="def"
                     /locus_tag="K8M77_000654"
     CDS             10336..10779
                     /gene="def"
                     /locus_tag="K8M77_000654"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI7085171.1"
                     /translation="MAIRNLRFNDDEILRKKCRVVDDINDRIKVLVEDMIETMYENNG
                     VGLASPQVGILKRIFVVDAMDGAGSRVFINPEILEKSGEQTDEEGCLSLPGRHKPVKR
                     ANKIKIKALDVNGNEFVLDAEGFLARAIQHEYDHLEGVLFIDHEL"
     gene            10799..11728
                     /locus_tag="K8M77_000655"
     CDS             10799..11728
                     /locus_tag="K8M77_000655"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBI7085172.1"
                     /translation="MKIVFMGTPDFAVPSLKSLINEFGVEAVFTQPDRPKGRGKKLGM
                     SPVKEVALEHNIPVYQPLRLKNEPETIEELKNMEPDFIIVVAFGQILPKEVLDIPKYG
                     CINLHASLLPKFRGAAPLNWSIIKGEKVTGNTTMLMDVGLDTGDMLLKDEVEITDNMT
                     AGELHDILMERGGELLVRTIKGILNNEITPEKQNEEETCYAPMLNKEIAKIDWSLSAQ
                     DIHNLVRGLNPWPVALTSYDDITMKVHQTRVEKGESNKEPGTIIAVDKTGIKVSTGKD
                     ILVIEKLQFPNSKQLFVEQFINGNTIEVGKVLK"
     gene            11746..12441
                     /locus_tag="K8M77_000656"
     CDS             11746..12441
                     /locus_tag="K8M77_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBI7085173.1"
                     /translation="MFYPYYIDPTYLILIPAILISAWAQFKVSSTFNKYSTVRSINGY
                     TGAQVARILLNDAGLQEVEIQQVPGRLSDHYDPRAKVLRLSSDVYGSTSVASIGVAAH
                     EVGHAIQDKESYSALVFRNAIVPLVNFSSSLSWILFFIGILLSYSTLVTIGIILFSVV
                     VLFQLVTLPVEFNASSRALKLLEARGILYDKEVDGARNVLSAAALTYVAATLMAVLQL
                     VRLIAISNRNSND"
     gene            12452..13780
                     /gene="rsmB"
                     /locus_tag="K8M77_000657"
     CDS             12452..13780
                     /gene="rsmB"
                     /locus_tag="K8M77_000657"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBI7085174.1"
                     /translation="MNARKIIVEILDNVLLNGAYSNIEINKQFASNDIDPKDKGLITE
                     VVYGTIKYKKMIDIILSNFVADIGKIDESVVNILRSAIYQMKFLDRVPPYAIVNEAVN
                     LTKETEPNLAKFVNGVLRNYLRNENKNFKVGLRNNEALCYDFSFDRWMIEMFIKQYGK
                     DDALRILRGLNTIPYVTVRVNTCKADYDEVYERLEEEGYDIEEGAFSPEAIIIKKGSA
                     IEKNKLYQEGLITVQDESAMLVAPLFDLKDDEQVMDLCSAPGTKATHIGELMMNKGKV
                     VAFDIHDHKLTLIKENIDRLGLTNVEVELGDATKINSKYINWADRVLLDVPCSGLGII
                     RKKPEIKWNKKNNDLTEVVKVQKEILKNAWNYLREGGELVYSTCTLNKKENEEVIDWF
                     VEKNSDCEVEKVFLGKADNVVYNDNGSVTILPNKYMDGFFIAKLKKKESK"
     gene            13812..14855
                     /gene="rlmN"
                     /locus_tag="K8M77_000658"
     CDS             13812..14855
                     /gene="rlmN"
                     /locus_tag="K8M77_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBI7085175.1"
                     /translation="MKNILDFTLEELKEWMKENGENAFRAKQIFDWIYKKEVFNFEEM
                     KNISKALIGKLSENFYIGIPEVIDYLSSSEDGTRKILLGLGDGNIIECVIMKYKYGNS
                     ICVSTQIGCRMGCKFCASTLEGMVRNLTAGEILSEVLIGQKLLGERISNIVLMGSGEP
                     LDNYDNVMKFLEIVNADYGLNIGQRHITLSTCGLVPKIREMADKEMQVTLAISLHAVS
                     DEKRKTIMPIANKYSISEILDACNYYIEKTGRRITFEYSLVSGVNDTKEDAKSLGRLL
                     KGMLCHVNLIPVNEIKENEFKKSTKKDIETFLNTLKTYGVEATVRREMGSDINAACGQ
                     LRRSYIKSKGLKL"
     gene            14868..15587
                     /locus_tag="K8M77_000659"
     CDS             14868..15587
                     /locus_tag="K8M77_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBI7085176.1"
                     /translation="MLGFVTDKGNVRELNEDFLSFSREDEFSIYVVGDGMGGHNAGEV
                     ASKRAVESIICFVKENFGVIPLDNLLKDAILHANSKIYSMSKESSGLSGMGTTITAIL
                     EVKNIVYIANVGDSACFGINENGIKKLTKDHSLVQELVDSGCITEGEAKNHPRKNVIT
                     RAIGTNSNVDVDVFKVDRHEYKAYLLCTDGLINDVDEDEIWKKVIKSNDFDKACNELV
                     EMAKARGGKDNITVLVFGGEK"
     gene            15589..17559
                     /gene="pknB"
                     /locus_tag="K8M77_000660"
                     /pseudo
     CDS             15589..17559
                     /gene="pknB"
                     /locus_tag="K8M77_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
     gene            17855..18718
                     /gene="rsgA"
                     /locus_tag="K8M77_000661"
     CDS             17855..18718
                     /gene="rsgA"
                     /locus_tag="K8M77_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBI7085177.1"
                     /translation="MEGIIIKGIGGFYYIKTDEGIIECKARGKFRYNSLKPMVGDRVT
                     IKVENGKGVIEDIHERSSELIRPTVANVTQAFVVFAIKNPDINLDLLNRFLTLCEYND
                     IHAVVCLNKEDLCTDEEKENLKELINDIGYEVLFINAKEGKGFDALKERLEHNITVLC
                     GPSGAGKSTLLNSFIDREHMETGSVSEKIGRGKHTTRHSELIDVDNGYLVDTPGFTTL
                     DVTFIDRDSLKYCFPEFNDYNNLCKFNGCNHYKEPKCAVKEAVEEGKINKLRYEFYIK
                     TLEEIINRRGN"
     gene            18718..19371
                     /gene="rpe"
                     /locus_tag="K8M77_000662"
     CDS             18718..19371
                     /gene="rpe"
                     /locus_tag="K8M77_000662"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBI7085178.1"
                     /translation="MVKIAPSILSADFSILGEQVKRVEKGGAEFLHIDVMDGHFVPNI
                     SFGFPIMKAIRNLSNLVFDVHLMIEEPGRYVDEFISAGADLITVHYESDRHIDRTINY
                     IKSKGCKAGIALNPGTPVSMLKNLIPSLDMVLIMSVNPGFGGQSFIPYSLDKIKEVKE
                     IAKACGKEDLLIQVDGGVGENNIKEIVEAGANVLVAGSAVFKEDKIEENIKALREAL"
     gene            19373..20002
                     /locus_tag="K8M77_000663"
     CDS             19373..20002
                     /locus_tag="K8M77_000663"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBI7085179.1"
                     /translation="MKVLIIAGGEKPSSDLLKSEIKECDLLIAADRGAEAYLENGFTP
                     DYALGDFDSIGEEYKYVLDKIKVEKFNPEKDNTDTEIAFFKAVELGATEIVFLGVTGT
                     RLDHVMANLGLLREALERNINACIIDNNNKIYLKNKRTTLKKEFGNYISFQAFGSPVD
                     NFSIKGSKYELYNHKLLIGDSLCVSNEFIDEYIDISFEKGEVLVIYSRD"
     gene            20068..20151
                     /gene="spoVM"
                     /locus_tag="K8M77_000664"
     CDS             20068..20151
                     /gene="spoVM"
                     /locus_tag="K8M77_000664"
                     /inference="COORDINATES: protein motif:HMM:NF033436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein SpoVM"
                     /protein_id="HBI7085180.1"
                     /translation="MRIMAIKLPKFLAKIVRMFKGNKKSDT"
     gene            complement(20245..20436)
                     /locus_tag="K8M77_000665"
     CDS             complement(20245..20436)
                     /locus_tag="K8M77_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBI7085181.1"
                     /translation="MARRCEICNKGVVAGVQYSHSHRQSKRTWAPNIKKVKAIVKGTP
                     KTVHVCTRCLRSGKVQRAI"
     gene            20695..21042
                     /locus_tag="K8M77_000666"
     CDS             20695..21042
                     /locus_tag="K8M77_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBI7085182.1"
                     /translation="MIGYSNEYGNVSYTDDVLAKIVGLSTMECYGVVGMVSKSATEGL
                     WELMRIENLTKGVKIDDKNDKLTIEVFVMVEYGTKISVIANNIIQKVKYNVENYTGLK
                     VSSITVNVQAVRV"
     gene            21056..22702
                     /locus_tag="K8M77_000667"
     CDS             21056..22702
                     /locus_tag="K8M77_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBI7085183.1"
                     /translation="MKFNKVNGEHLYYMMSNAANKLELQSEYVNSLNVFPVPDGDTGT
                     NMSMTFRAAVKEIEGMDNKNIGEVSKKLAKGALMGARGNSGVILSQILRGISKGLEGK
                     EEADASEFANALLEGSKSAYKAVMRPTEGTILTIVRIAGEVAVALKEDNITELMREVC
                     RESKIMLDKTPEMLPALKKAKVVDSGGMGLLIILQGMQEALENGLKVTTGTPQAVKSS
                     AVKAQRSETMSEEDIKFGYCTEFIILGDSNHAEEFKSKVINKGDSLVVVGYEDVIKVH
                     IHTNNPGKVLEEAVKFGELSKIKIDNMREEHREMLEGMYEEAAETEESVAAGEFKKYA
                     FISVAMGEGMKNIFKDLGVDYVIEGGQTMNPSTQDMLEAIEKLNAEHIFILPNNKNII
                     MAANQAAEISDKDIRVIPTKTIPQGITCITMFNPEADVEENTEELKEAMEMVKTTSVT
                     YAVRDTEVDGKEIKEGNILGLVEGKIKEVGEDPYKVAEDLIDSLVDEDSELITIFYGK
                     DCEEEKVDALIEKLEGKYEDLDVQCYKGEQPLYYFIMSVE"
     gene            22892..24925
                     /gene="recG"
                     /locus_tag="K8M77_000668"
     CDS             22892..24925
                     /gene="recG"
                     /locus_tag="K8M77_000668"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017826132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBI7085184.1"
                     /translation="MNIYDDIGLVKGVGPKLKERLNKVGIFTVLDLLLYFPRDYEFLN
                     DDISLNGDTSDEKAILKCKVKSYGSSIKTRNGKTLTTINFTYNNLKVIGKWFNQPYIK
                     RNFILGNEYNLMGKFKKVNNTLEVINPLIPCKEANKSEILPIYTLKNGLTNKILVKLI
                     NEILKNMIIKENLPEEIVKKYKLISLDKAIRNIHFPVGRGELQSAINRLKFQELFTYS
                     LKIIMMKAHIKKENSGISFKMSPLLKDLKESLPYTLTNAQSRTLREILLDQKRNIAMN
                     RLVQGDVGSGKTLVALISMFNVYMNGYQTVLMAPTEILANQHYAEAKKYLDKFGVDIE
                     LLTGSTKEKEKKRIKEKIASGKEIMLIGTHALIQDDVELNNLGLVVTDEQHRFGVEQR
                     SRLINKNKRADVLVMTATPIPRTLSLYLYSDLDISIIDELPPGRKPIDTMLVDMNQRM
                     KAYNFALNEVEKGRQFYIVSPLIEENEKLNLNSVEKIYEELKNGIFKDVRIEILHGKM
                     AGKDKDKIINTFKNGEIKGIISTTVIEVGVNVPNSTMMIIENAERFGLAQLHQLRGRV
                     GRGEHKSYCILIANTKNDITRRRMKIMTESSDGFYIAEEDLKLRGAGEVFGMRQHGDE
                     GFILANVVDDINILKCANHEAKLIVNNINEDNKKLCTEIMRGIERNSRYICFN"
     gene            24991..25548
                     /gene="rsmD"
                     /locus_tag="K8M77_000669"
     CDS             24991..25548
                     /gene="rsmD"
                     /locus_tag="K8M77_000669"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBI7085185.1"
                     /translation="MRIIAGKARGRKLLSPPTYETRPTLDRVKESMFSMIQWYIPDAV
                     VIDVFAGTGSLGLEAASRGAKECYLVDKSPVTFPVLKKNIENLGFGNFCHALNTDAYS
                     ALRSLASRGKVFDLMFIDPPYMKNLIPEAIEIIEEKNLLQEDGLIVTKIDSSEEIFEG
                     TEKIKLTKVKKYGNTTVCFYKIEED"
     gene            25550..26044
                     /gene="coaD"
                     /locus_tag="K8M77_000670"
     CDS             25550..26044
                     /gene="coaD"
                     /locus_tag="K8M77_000670"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBI7085186.1"
                     /translation="MRVGVYPGSFDPITKGHLDLIERAASKFDKVIVAVLININKKGM
                     FSIEERVNLIEKCVAKYNNVEVKSFNGLLIDFVRKEKADVIIKGLRSVTDFEYEFQMA
                     LMNRELANEVETVFMVTSPNYSYISSSAIKQVASFNGEIKNFVPKEIVEDLEERIISL
                     RGEG"
     gene            26053..26583
                     /locus_tag="K8M77_000671"
     CDS             26053..26583
                     /locus_tag="K8M77_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI7085187.1"
                     /translation="MESQVIGILDYLRDIVEGASKVPITGKVVVDRKEVLELLDQVIN
                     CLPDEIKKAQWVLGEKERILAEARKENESVKQETYEMMKKRIENHNIVKEAELRAQEI
                     IANAQRQAKTIRLGSREYADEILSQLEIEITNKREEFLNHMKNNMEKFAIDFTEDLDK
                     ATGEIRNNIKELRGDK"
     gene            complement(26566..27726)
                     /gene="ylbJ"
                     /locus_tag="K8M77_000672"
     CDS             complement(26566..27726)
                     /gene="ylbJ"
                     /locus_tag="K8M77_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBI7085188.1"
                     /translation="MYSILFLLIIILSITLIKLLKIKGNIIFSLFLTLTVIYFIVNPK
                     LSMEASLAGAKLFFYSVLPTMFPFMVICNMIINLDGIKLYSKILGPILCKPLGLSYPC
                     SFALVASFLCGYPLGAKYSTDLYKKELIPHDEFVRLLNIASNIGPLFLLGAVGTSMLG
                     NSTLGYLLLIPSYLSAIIMGIITKNKKREKKLSLSKEVITETKSTFNIGEVIKKSIED
                     ASLNILILCGYVIMFSVIISMLKTLFISTGTLTNLSSILNIPPDIFNGLFLGSIEVTN
                     GCNIIASSNLSLFSKLLLISFLSAFGGLSIIMQTSSFFYKENVSIIKYFLFKVLQGII
                     AFVLMFFVYIIFKNNIAVSLIESSFLITLSPIILIIGITLGLAILYKLLVAS"
     gene            complement(27791..28999)
                     /locus_tag="K8M77_000673"
     CDS             complement(27791..28999)
                     /locus_tag="K8M77_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBI7085189.1"
                     /translation="MNITGIITEYNPFHLGHELHLKNSKEITNCDGVICVMSGNFVQR
                     GLPALTDKWTRTKMALEAGVDLVVELPTLFATSSAEFFAFGAVSLLNSLNVVNNICFG
                     SECGDIDLIKKLSEIIVNEPPIFKEYLKDYLKEGLPFPKARSEALMKYLDYNNYKTDF
                     SCLEKVLNSSNNILAIEYCKSLYKLQSTIKPFTIQRLGADYNDEELSKNEIASASAIR
                     KIIYTSNIEESFDFMPEYSYNLLKNTSFSDLDKMFDLVKYAIVSNPNILKEIPEASEG
                     IDNKIIQNIGKANSLDELINLCKSKRYSYTRLNRILCHILLNVNKDLLSLRKYSPNYV
                     RILGFNNKGREILKEIKKNSEINIVNKLSKAKTDPLLEFDIKATNIYSFLNPSVKINS
                     DYLISPIIFR"
BASE COUNT        11510 a         3007 c         5497 g         9029 t
ORIGIN      
        1 aataaaaata tttaaaataa atagcattaa tttagatttt tttgaataaa taatattaat
       61 acataatatt agaaagagaa agttgaagtt taaataaagg agttaaaaag tatatacaat
      121 taaagtaaaa atatattttg ctaaacagta tactttttca aagtttctaa tatatatata
      181 gtgttaataa tatttatagg agggtgtatc ttggaagatt tcaatatata caaagatata
      241 gcagaaagaa cacagggaga tatttatgtt ggagttgttg ggccagtaag aacaggtaaa
      301 tctacattta taaaaagatt tatggactta atggtaatac ctaagattga taatgcttac
      361 aaaaaggaaa gagcaaagga tgaattacca caaagtggat caggaaaaac tattcataca
      421 acagagccta aatttgtacc taatgaggca gtagaaattg ctttagatga tggcattaag
      481 tttagtgtga gaatggttga ttgtgttgga tacattgtta aaggggctaa tggttatttt
      541 gatgatggag aatctaaaaa agttcatact ccttggtttg actatgaaat tccatttgaa
      601 gatgcagcag agataggaac tagaaaagta ataacagacc attcaacaat aggattagta
      661 gttactacag atggaagtat aactggtata gatagggatg attacttaga tgctgaagaa
      721 agagttgtag ctgagttaaa atcaatagac aaacctttta taattgtact taattcatta
      781 gatccaaggg cagaagagac tttagactta aaacaagaat tagaaatcag atatggagtt
      841 ccagttcaaa taatggatgt agccaatatg aatgaaaatg acataaacga tttatttaca
      901 aaagtactta aagaatttcc agttaaggaa attaatatag acatgccaaa atggattgaa
      961 aaattagagc cttctcattg gttaaaatct aattttatag acatagttaa agacatgtgt
     1021 aaaaacatat caaaaattag agacgttaag gatctactta gtacttatgg agaagatttc
     1081 ttaggggtag cagatataag tgagatgaat ttaggggatg gaactgtaag agttaaaatg
     1141 actcctaaaa atggtatctt ctataaaata ataagtgaaa tgtgtgatga agaattaaat
     1201 gatgaaagtg atttaatagc tttaatcaaa gatttgcata aagcaaaatc tgaatatgat
     1261 aaggtagctg aagcaataaa tagtgttaag gaaacaggtt atggactagt tgctccgcaa
     1321 ttatcagaaa tgaagtttga aaaaccagat attgataagc aaggttcaaa atatgttgta
     1381 aaacttaagg ctagtgctcc tagtcttcac ttaataaaag cagatattca aacagaaatt
     1441 tgcccaataa tgggaactga aaaagaaact caagaggtat ttaaaacatt acttgagcaa
     1501 tttgaaagcg atccggaaaa attatggcaa agcaatatgt ttggcaagtc cttagagaca
     1561 ttagttcaag aaggtttaag aagtaaactt tataagatgc cagatgatat tcaaagcaag
     1621 attcaaaaaa ctcttcaaag aatcattaat gaaggggaag gaaatttaat ctgtattatt
     1681 ttctaagttg attaatatat tcaaagacca tatcaaattt tattttgata tggtctttta
     1741 aatttttaat gaagtaattt tatgaatttt agaatgtgaa aaatacatat aagtattttt
     1801 ataatatatt tattttgcga aaagaagatt atattaaaaa atatgcttta atttattagt
     1861 tttataaaat ttgtaaaaag attataatta ttaggggaat gattaagtta ttagggctta
     1921 ttagtattta atataagttt agctttgaaa agattgaata gaattattaa tttaattata
     1981 ataagcatta tgacgaatga tacttagtca aataaaaaaa gaaattcgga ggtactcatg
     2041 aagaaaatag ctgtaatttt taatggtgga acaatatcaa tgaaagttga tgataggata
     2101 aaggctgcag ttccaagttt gacaggagag gaaataatgg ctatggtaac aggaatagaa
     2161 agctttgcaa caatagagtc acatagcttt tcttcattgc caagtccaca tattactcca
     2221 gagataatgc ttgaactatc aaaatttata aattctttag tagagagaga agacatagat
     2281 ggagttgtag taactcatgg aacagatact ctagaagaaa catcttattt tgttaattta
     2341 acaactaaga caagtaaacc aatagttttt actggagcta tgagaagtgg atcagagtta
     2401 ggatatgatg gaccatctaa tttagctgca tcaatatgta cagctgcatc agatgaagca
     2461 aaaaatagag gagttctagt ttgttttaat ggggaattaa atagtgcttc agaggtaact
     2521 aaggctaatt caatggcttt aaatgctttt agaacaccta attttggccc aataggaata
     2581 gttgataata atagggtaat atttgataga agagttccac aggaagattt tataccctta
     2641 gaggttattg ataaaaaagt agccataata aaatgtgctg caggtatgga tggagatttt
     2701 atatatcact gtatagagaa aaaatatgat ggaatcataa tagaagcttt aggaagagga
     2761 aatgtaccac caactatggt tgatgcaata aaagatgctt tagataatgg aatagttata
     2821 gtacttactt caagatgttt tgaaggaaga gtaagtgata gttatggata tcttggaggt
     2881 ggaaaaaatc ttaaagagct tggtgttata tttggagaaa gtatgccaag tcaaaaggct
     2941 agaataaagc ttttaactct tcttggattt aataaagatt atagatatat taaacatagt
     3001 tttgagaagg aaagatatta aacacatata ttttaaaaat aataataaaa aaatattcga
     3061 taattattga ataataactt agaatagtgt acaataacat tcatagacag atgaaaagtt
     3121 cattttatat aataatgttc gtgttttaat gagggatgtt aatagtaaac ttgattaaag
     3181 ttattattca ataattggat aaaagctatt ttaagtttac taaacttaaa atagcttttt
     3241 tatatttatg aacttaagag gaatatggag gttttttatg gaaaataaaa ttcatgcttt
     3301 aagtgaagaa ggaaatttac gtgttttacc tgagaataaa agtgaatata agagagaatt
     3361 cttagctgga acaacaagtt tcttagcaat ggcttatata atagctgtaa atccatctat
     3421 attaagcgca gcaggaatgc cagcaggtgc tatagtaacg gcaacatgta tatcagcagt
     3481 tattggatgt ttaataatgg ggttttatgc aaaactacct tttggactag ctcctggaat
     3541 gggactaaat gcattcttta ctttttcagt agttatagga atgggaattt cttgggaagt
     3601 agctcttaca gctgtttttg tagaaggaat aatatttata cttttatcat tatttaaagt
     3661 aagagaggct gttgttgatg caattccaat aaatttaaaa tatgcagtta cagcagggat
     3721 aggtcttttc atagctttca taggatttaa tggagctgga gttgttattg gaaatccaga
     3781 tacaatggtt gctatgggac aagttggtcc taaaatgtta atagcaatgg ttggactttg
     3841 tataatagta attttagaaa agaaaaaagt taaaggttca atgctagttg gtatagtagt
     3901 ttcaactctt ttagcttggg gatatgcttc aataaatact gaagccgcag ctagtatggg
     3961 aatctatttg ccaaatggaa tatttaaatt tgaatcaata gctccaatag caggtaaagt
     4021 taatttttca tatttaactt caccacagca tgtatttaat tttataacta tagttttcac
     4081 atttttattt gttgattttt ttgatacagt aggaacttta ataggagtag cttcaagagc
     4141 taatatgtta gataagaagg gaagagttcc taatgcaggt aaagcattaa tgacagatgc
     4201 tatagcaact acggcaggcg ctttacttgg aacatctact gtaacagttt atgttgaaag
     4261 tgctactgga gttgaagaag gtggtagaac aggattaaca gctataacaa taggagcttt
     4321 atttttcgta gcaatgttct tttcaccaat atttgtatca gtaccagcat gtgctactgc
     4381 accagcttta atatatgttg gatatttaat gctaactagt gtgttaaaaa tagactttag
     4441 tgatattaca gatgcagtac cagcattttt aataatagct ttaatgcctc taacttatag
     4501 cataggtgat ggattaacaa ttggtgtttt aacatatgta atattaaata tattacacaa
     4561 tatctttgct aaaaacaaag aagataaaaa agaattatca atggtaatga tagttttagc
     4621 aattatattt gtaataaaac tttgtctacc attaattact caaatgatag gttaagataa
     4681 aagtgaattt tatttagaat atgtacttta aaagacttta ttataagatt gagagttatt
     4741 taataaaaaa taatttgaga tttaggtaaa gtctttttta ttttgaagaa ataattctta
     4801 aaaaataaat gttaacaaag tgtaaaaaat aacttgttat tacttgaatt ttatattaat
     4861 ctatatttag attattttca aggaggctct ctttatggtt aaaagtatga ctggttttgg
     4921 gagagcaact agtgaagagg gaaaagaaag aatattttct ctagaaatga agagtgtaaa
     4981 ccatagatat ttagacatga acataagaat gccaagaagt atggtttctt tagaagaaaa
     5041 aataagaaat attatttctt ctaaacttag caggggaaaa gttgatatct ttataaatta
     5101 taaagattat gctaaaaatc aaggagttgc tgttttaaat gaagatttag ctaaaagtta
     5161 tgtaaattct ttagaacaat taaagtcctt gtttcctaat atgcaagacg acttaagttt
     5221 atcattagtt gctagatacc cagatgtaat aactatagaa gaaaaatcag aagatttaga
     5281 agctatatgg gaagagataa actcactatt aaatatggct gtagaaaata tgatttctat
     5341 gagaaaagtt gaaggtgaga aattagctag tgatatatta gttaagtgta gctctataga
     5401 agaaatagta gcttttatag aagaaaagtc agaggttatt gttgcttctt ataagcaaaa
     5461 gttagaggat agacttaaga atttattagg ggaagttcca gtagatgaaa acagagtggc
     5521 tatggaagtt gctgtttttg ctgacaaagc ttcaattgat gaagagataa taagacttag
     5581 aagtcatatt aatcaattaa gaaaaacttt aactttagat gaacctatag gtagaaagct
     5641 agatttcata gtacaagaaa tgaacagaga agctaataca atagcatcaa agtcaacaga
     5701 tttagaaata accaacaagg ttatagatat aaaaaatatt attgaaaaga ttagagaaca
     5761 agttcaaaac atagaataat aggaggtagc aaatgagtat aaagttaatt aacattggtt
     5821 ttggaaacat agtttcagct aatagattag tagcaatagt aagtcctgaa tcagcaccta
     5881 taaagagaat catacaagaa gctagagata gaggtatgct tatagatgca acttatggta
     5941 gaagaacaag agccgtaata attacagatt cagatcatgt aattctttca gctgttcaac
     6001 ctgagacagt ggctcacaga ttatcaacta aggaagaagt agttgttgaa gatgatgaat
     6061 aagatacata agaataatag aggtgtatta atagttattt ctggtccttc aggtgcaggt
     6121 aagggaacta tttgtaaagc cttattagaa aaacacgatg atatattcat atcagtttct
     6181 gctacaacta gaaaccctag agtaggagaa gttgatggag ttaattatca tttcttaaca
     6241 aaagaagaat ttaaacaaag aatagcagag gatgatttcc ttgagcatgc tgaagtatat
     6301 ggaaattatt atggaactcc taaatcaagt gttgaaaaaa tgcttgatga aggaaaaaac
     6361 gtaatattag aaatagatat acaaggtgct ttaaaagtta aggaaaaggc tactgatgga
     6421 gtatttatct ttatattacc tccttcaatg gaagagctta agcaaagaat aataaaaaga
     6481 ggtagcgaaa ctccagaaag tttaatgact agatttaagt cagcttataa agaaattaat
     6541 tatgtgtcaa aatacaatta tgcagtagtt aacgataatg tagaagatgc agtaaagaaa
     6601 atagaagcta tattattagc agaaaaatgt agagtagata gattaaaaga aaacttatta
     6661 gagtcaaagg aggacgaaat gcatgaacaa ctctatgatt aatccatcaa tcgttgattt
     6721 attaaaaagg gtagaagata gatattcttt agtaatatta agtgctaaga gagcaagaca
     6781 aataattgac ggagcagaaa cttttgttga tgttgagtca aataagccat taacaatagc
     6841 tataaatgaa atagatgaag gatttgtaaa ttacaaagac actgaggaga aataagagct
     6901 atgaaagata agaaatgtgt tgttgtagga gtaagcgggg gagtggctgt ttataaagcc
     6961 ttagacgtca taagcagact aagaaaaaaa gatgtagaag tacatgtaat aatgactaaa
     7021 tccgctactg agtttgtaac accattatct tttcaatcat taagccaaaa catggttata
     7081 atagatatgt ttgctgaacc aaaggcttgg gaaatacagc atatatcact agcaaagaaa
     7141 gcagatttaa tgcttattgt accagctaca gcaaacatta taggaaaggt tgcaaatggc
     7201 atagctgacg atatgttatc aacaactata atggcaacaa aggcgccagt tattttttgc
     7261 cctgcaatga atacaaatat gtatgaaaat cctatagttc aaaggaacat aaatcttcta
     7321 aaggagtttg gttatgaatt tatagagcca gctagtggaa gattagcatg tggtgatgag
     7381 ggaaaaggta aacttcaaga tgccgaaatt attgcagaag aaacattgag aaggttacat
     7441 tctactaagg atttacttgg taagaaggtt gttgttactg caggacctac tatggtgcca
     7501 atagatccag taagaatttt aacaaataga tcttcaggta aaatgggata ctcaatagca
     7561 gaggaagcga gagatagggg agcagaagtt gttcttatat caggacctac ttctttaata
     7621 aaacctaacg gaattaaagt tattgatata aagacaaatg aagatatgtt taatgctata
     7681 aaaaatgaat ttaaagatgc tgatatagtt attaagtcag cggctgtggc tgattataaa
     7741 gctaaaaatt atagcaatga aaagattaaa aaaacaggtg atgatttaaa tttaattttt
     7801 gagagagata gagatattct aaaaactctt ggagatatga aagaaaatca aattctagta
     7861 ggctttgcag cagaaagtag caacttaaaa gaaaatgcta aaggtaaact agagaggaaa
     7921 aatctagatt atattgttgc aaatgacata agtaatcaag aaacaggttt tgcaagtgat
     7981 gaaaataaag tcactataat aagcaaatct ggtgaagagg tatctttaga aaaaatgtct
     8041 aaaagagaag ttgcaaagaa tatatttgat attataaaag ggcgctaatt gcgccttttt
     8101 attctatata aacaagggtg attattttgg agaaatttgc tgaagtaatt gtaaatagtg
     8161 aagctgttac aattgataag ccttttacat ataagattaa agaagattta gttcataata
     8221 taaaggttgg acatagagtt ttaattcctt ttggaaatgg aaataaaaaa attgaaggtt
     8281 ttgttttaaa aatattagat aatgttgaaa ataaaagtat aagatataaa tctatatata
     8341 atgtttgtga taatgaaccc ttattaagtg aagattcttt aaagcttgtt aaatttttaa
     8401 gagaaaaata tttatgtaag tatatagatg ctataagagt tatgattcca cctaaaataa
     8461 tgagtggaaa taaggagaaa acaaaacaag ttatagtatt taaaaaatat atagaagaat
     8521 taagtgagcc acaaaaaaga gctttagagc taattgaaga aaaaagtggt ggttttacaa
     8581 aagcagaatg gaataaaatt tttaatatat caacatatat gataaataaa ttaatagaat
     8641 taggttgtgt ttctagtgaa gaagtaaggg ttggaaggga aaatttaaga gagtttaagc
     8701 cttatccgcc taagagttta aatgaagaac agataagagc atatgataca attttagaaa
     8761 gtgataaaaa tatattttta ttaaagggcg taactggaag tggaaaaaca gaagtatata
     8821 tgaatttagt tagttatatg ctcctttttg aaaagagttc attaatatta gtaccagaaa
     8881 tagctctaac tcctcaaatg attgaaagat ttaaaggaag atttggaaaa gatgtagcgg
     8941 tctttcatag taggctttca gatggagaga gatatgatga atggtatagg gttaaaaatg
     9001 atcaagttaa ggttgttata ggggcaagat ccgctatatt tttacccttt aatgatttag
     9061 gactaatagt agtggatgaa gaacatgaaa gtagttataa atcagatatg aatcctaaat
     9121 ataatactat agaagtttgt gagtatttaa gtgttctaaa aaattgtaag gttgttttag
     9181 gttcagcaac ccctagtgtt gttagttttt ataaagcact taaagggaat tatgaattaa
     9241 ttgaacttaa aaagagagtt aataatttag aacttcctaa tgtatctatt gtggatatga
     9301 gggaagagct aaaatcaaat aataaaacaa tgtttagtag ggatttatat ttaaagataa
     9361 aagatagatt agagaaaaaa gaacaaataa ttttgttctt aaatagaaga ggattttcat
     9421 cctttgtttc ttgtagaagt tgtggatttg tttttaaatg taacaattgt gacatatcaa
     9481 tgacttatca taataatgga tacttagttt gtcattattg tggaaatact gaaaagatgc
     9541 ctaagctttg ccctaagtgt ggtagtaact atgttaaaca ttttggggta ggaacagaaa
     9601 aattagagga agcagtacat catcatttta aagaagcaaa ggttttaagg atggatgtag
     9661 atacaacaag aaagaaaaat tctcatgagg aaatttataa ctcatttaaa agtaaagaag
     9721 cagatatatt aataggaact cagatgatag ctaaagggct tgatttccca gatgtaacct
     9781 tagttggagt tatggctgca gatatgtctt taaatttacc agattataag tctagtgaaa
     9841 agacatttca acttataact caggtaagtg gtagagcagg acgtggtgat aaaaagggag
     9901 atgtaataat tcaaagttat tctccagaac attatgcttt gacctgtgca agggataata
     9961 attatgaaga gttctttaaa agagaaataa atttaagaaa acttatggca tatccaccct
    10021 ttgatgattt gtttttaata aacttaagtg caaagggaga aataaaagta aagatttttg
    10081 caaaaaaatt agaggcgtat ttacgtaata tattaaaaga atatgataat atatttatat
    10141 acgaagcatg cccttgttct ataagtaaaa taaaaaatgt ttatagatgg caaattctaa
    10201 ttaagggaaa attagattta gaggtatgct ctaaaataaa aaaaggggtt tatgaattat
    10261 ccaaagatgt ttataatgag attaagattg gtttagatat aaatcctaat agcttaatgt
    10321 aaggaggaag ttattttggc aataagaaac ttaagattta atgacgacga aatattaaga
    10381 aaaaaatgta gagtagttga tgacattaat gatagaataa aagtcttagt agaagatatg
    10441 atagaaacta tgtatgaaaa taatggagtt ggattagcat ctccacaagt tggaatacta
    10501 aagagaatat ttgttgttga tgctatggat ggagcgggtt ctagagtatt cataaatcca
    10561 gaaatcttag aaaaaagtgg agagcaaact gatgaagaag gatgcttaag cttaccaggt
    10621 agacataaac cagtaaagag ggctaataaa ataaagataa aggctttaga tgttaatgga
    10681 aatgagtttg tattagatgc agaaggattt ttagcaaggg ctatacaaca tgaatatgat
    10741 catttagagg gagtactttt tatagatcat gagctttaag actaaggagg gatatgacat
    10801 gaagatagta tttatgggaa caccagattt tgctgttcca tcattaaaga gcttaataaa
    10861 tgaatttgga gtagaagctg tttttactca accagataga ccgaagggaa gaggaaagaa
    10921 attaggtatg tcacctgtaa aggaagttgc tttagaacac aacataccag tttatcaacc
    10981 tttaagatta aaaaatgaac cagaaactat agaagaacta aaaaatatgg agccagactt
    11041 tataatagta gtagcttttg gtcagatatt acctaaagag gttttagata ttcctaagta
    11101 tgggtgtata aatttacatg cttcattact tcctaaattt agaggagcag cacctttaaa
    11161 ttggagtata atcaaaggag aaaaggtaac aggaaataca actatgctta tggatgtagg
    11221 acttgatact ggtgatatgc ttttaaaaga tgaagtagaa ataactgata atatgactgc
    11281 aggagaatta catgatattt taatggaaag aggaggagag ttattagtta gaactataaa
    11341 gggcatactt aataatgaaa taactccaga aaagcaaaat gaagaagaaa catgttatgc
    11401 tccaatgctt aataaagaaa tagctaaaat tgattggtct ctttcagcgc aggatattca
    11461 taatttagtt agagggttaa atccttggcc tgtagcttta acttcatatg atgatattac
    11521 aatgaaagtt catcaaacta gagttgaaaa aggggagagc aataaagagc caggaactat
    11581 aatagctgta gataaaacag gaattaaggt ttcaacagga aaagatatac ttgttataga
    11641 aaaattacag tttccaaata gtaagcaact atttgtagag caatttataa atggaaacac
    11701 aatagaagta ggtaaagttt taaaataatt taagagggtg atggtatgtt ttatccatat
    11761 tatattgatc caacatattt aatacttata ccagcgattt taatatctgc atgggcacaa
    11821 tttaaagttt ctagcacatt taacaaatat agcaccgtta gaagtataaa tggatatact
    11881 ggtgcacaag ttgcaagaat tcttttaaat gatgctggac ttcaagaagt agaaattcaa
    11941 caagtaccag gaagattaag tgaccattat gatccaagag ctaaggtttt aaggttatca
    12001 tctgatgttt atggaagcac ttctgtagca tcaataggcg tagcagctca tgaagttggg
    12061 cacgctatac aagataaaga atcttattct gcattagttt ttagaaatgc catagtacca
    12121 ttagtaaact tttcatcaag cttatcatgg attttgtttt ttattggaat attactaagt
    12181 tatagtactt tagtaactat tggtataata ttattctcag tagttgtgct ttttcaatta
    12241 gtaacattac cagtagaatt taatgcctca tcaagagcat taaaactttt agaggcaaga
    12301 ggaatattat atgataaaga agtagatgga gctagaaatg tattaagtgc agcagcatta
    12361 acttatgttg cagcaacact tatggccgtt ttacaattag taagacttat agctataagt
    12421 aacagaaatt caaatgatta gaggttaaaa tatgaatgca agaaaaataa tagttgaaat
    12481 attagacaat gtcttattaa atggagcata ttcaaatata gaaataaata agcaatttgc
    12541 atctaatgat atagatccaa aagataaggg attaataaca gaggttgttt atggaacaat
    12601 aaaatacaaa aaaatgatag atataattct ttcaaatttt gttgctgata ttggtaagat
    12661 agatgagagt gtagtaaaca tattaagaag tgctatatat caaatgaaat tcctagatag
    12721 ggttcctcca tacgcaatag ttaatgaagc ggtaaactta actaaagaaa ctgaacctaa
    12781 tttagctaag tttgtaaatg gagttttaag aaattattta agaaatgaaa ataaaaactt
    12841 taaagttgga ttaagaaata acgaagcttt atgttatgac ttttcttttg atagatggat
    12901 gatagaaatg ttcataaaac aatacggaaa agatgatgct ttaagaatac ttagaggatt
    12961 aaatacaatt ccgtatgtaa cagttagagt taatacatgt aaagctgatt atgatgaagt
    13021 ttatgaaaga cttgaagaag aaggatatga tatagaggaa ggtgcatttt caccagaagc
    13081 tatcataatc aaaaaaggta gtgcaataga aaaaaataaa ctttatcaag aaggattaat
    13141 aacagttcaa gatgaaagcg ctatgttagt agctccttta tttgatttaa aggatgatga
    13201 acaagtcatg gatctatgta gtgcaccagg aactaaagca actcatatag gcgaattaat
    13261 gatgaataaa ggaaaagtag tagcttttga tattcatgac cataagttaa ctttgataaa
    13321 ggaaaatata gatagattag gattaactaa tgttgaagtt gaattaggag atgctacaaa
    13381 gataaattct aagtatataa attgggctga tagagtatta ttagatgtac cttgctcagg
    13441 tcttggaatt ataagaaaga aaccagaaat aaaatggaat aaaaagaata atgatttaac
    13501 agaagttgtt aaggttcaaa aagaaatatt aaaaaatgct tggaattatt taagagaagg
    13561 tggagaatta gtttactcta cttgtacttt aaataaaaaa gaaaatgaag aagttataga
    13621 ttggttcgta gaaaaaaatt cagactgcga agtagaaaaa gtatttttag gtaaggctga
    13681 taatgttgta tataatgata acggaagtgt taccatatta cctaataagt acatggatgg
    13741 tttctttatt gctaagctta agaaaaaaga aagtaaatag caaagaggat aaacatggaa
    13801 ggtgagactg tatgaaaaac atcttagatt ttacattaga agagttaaaa gagtggatga
    13861 aggaaaatgg agagaatgct tttagggcaa agcaaatctt cgattggata tacaaaaagg
    13921 aagtatttaa ttttgaagag atgaaaaata tttcaaaagc tctaataggc aagctttcag
    13981 aaaattttta tataggtatc cctgaagtta tagattattt atcatctagt gaagatggta
    14041 ctagaaaaat ccttttagga ttaggtgatg gaaacataat agaatgtgtt attatgaaat
    14101 ataaatatgg caattctatt tgtgtttcca cacaaatagg atgtagaatg ggatgtaaat
    14161 tctgtgcatc taccttagaa ggaatggtaa gaaatttaac tgcaggagaa atattatcag
    14221 aagttttaat tggacaaaaa cttctaggtg agagaatatc taatatagtt ttaatgggaa
    14281 gtggagagcc cttagacaat tatgataatg ttatgaagtt cttagagatt gttaatgctg
    14341 attatggttt aaatataggg caaagacata taacattatc aacatgtggt cttgtgccaa
    14401 aaattcgtga aatggctgac aaggaaatgc aagtaacttt agcaatttca ttacatgctg
    14461 tttcagatga aaagagaaaa acaataatgc caatagctaa taagtattca attagtgaaa
    14521 ttttagacgc ttgtaattat tatattgaga aaacaggaag aagaataact tttgaatatt
    14581 ctttagttag tggagttaat gatactaaag aagatgccaa gagcttagga agattattaa
    14641 aaggcatgct ttgtcatgtt aatttaattc ctgtaaatga aataaaggaa aatgagttta
    14701 aaaaatctac taagaaggat atagaaacat ttttaaatac tcttaaaaca tatggagtag
    14761 aagctacagt aagaagagag atggggtcag atataaatgc agcctgtggt caattgagaa
    14821 gaagctatat taagtcaaaa gggttaaagc tgtaggggga aatatttatg ttaggatttg
    14881 taacagataa aggaaatgtt agagagttaa atgaagattt tttatctttt tctagagaag
    14941 atgaattttc aatatatgta gttggtgatg gaatgggtgg acataatgct ggagaagtag
    15001 ctagtaaaag agctgtggaa agcattattt gttttgttaa agaaaatttc ggtgttatac
    15061 cattagataa tcttttaaaa gatgcaattt tacatgctaa tagtaaaata tatagtatga
    15121 gtaaagaaag ttcaggtcta tctggtatgg gtacaacaat aacagctata ttagaggtta
    15181 aaaatatagt ttatattgct aatgttggtg atagtgcttg ttttggtatt aatgaaaatg
    15241 gaataaaaaa attaacaaag gaccattcct tagttcaaga acttgttgat agtggttgca
    15301 ttacagaggg ggaagctaaa aatcatccaa gaaaaaatgt aataactagg gctattggaa
    15361 ctaattcaaa tgttgacgtt gatgtattta aggttgatag acatgaatat aaagcatact
    15421 tactttgtac agatggatta attaatgatg ttgatgagga tgaaatttgg aaaaaagtaa
    15481 ttaaatctaa tgattttgat aaagcatgca atgaattagt tgaaatggct aaagcaaggg
    15541 gaggaaagga taatattaca gtgttagttt ttggaggaga gaaatagaat gataggtaaa
    15601 atcttaggga ataggtacga attacttcaa tgtgtaggtg aaggtggtat gtcctttgtt
    15661 tataaagcaa aatgtagaaa attaaataga ttcgtagctg ttaaaatact aaaagatgaa
    15721 tttaaaaaca atgaagaaat tgttagaaga tttaaaaaag aagctacagc tattgctaac
    15781 ttatccaatc caaatgtagt aaatgtatta gatgttggaa ctcaagatga tattaattat
    15841 attgttatgg aatatattga aggtaaaact ttaaaagata taattaatga aaaaggtgct
    15901 ttaccttatg aaattgccat aagtatagga ataaaggttg ctaaagcttt agaatgtgcc
    15961 cataaaagtg gaattataca tagagatgtt aaaccacaaa atatattagt tacagaagaa
    16021 ggagttgtta aagtaacgga ctttggaata gctaagtcta tggattcatc tactatagca
    16081 cacactaata gtgttatggg gtctgcacat tatttttcac cagagcaagc aaaaggaaca
    16141 tatactgatt atagaactga cttatattct ttaggaatag tattatatga aatggttact
    16201 ggagttgttc cttttaatgg tgattcgcct gttactgtag ctgtaaaaca tatacaggag
    16261 aaagcaatac cacctaaaaa tataaatcaa aacattccta atagtttaaa tgatttaata
    16321 atgaaagcta tggaaaagga tcctgttaat aggtatcaaa cagctaaaga aataatagga
    16381 gacctagaaa agataaagaa ggatccgaat gttacaatat catcaaaatc tgcagaagat
    16441 gaagatcaat ttacaagagt tatgtctcca gttgttgttc caaatactga aactaataac
    16501 ttagaacctg atgaagatga tgaggatgat gatgaatatt atgaagagga tgaagatgaa
    16561 gaagaaaata atgttcaaac taaacctcaa aaagctatta ataaaaagaa aaaaaaatca
    16621 ccaatattaa ttgtaattgc aacaatttta gttgtagcat tagggataac tcttggattc
    16681 cttggtatga agaaatttat ggaaggtgga aaagatgtta aaatacctaa tgttgtagga
    16741 gaaaaagtag aagatgctaa gagtaagtta gaaggattag gattaaaggt attagaagtt
    16801 acagaagaaa gtgatcaaga aaaaggaata gttttaaaag ttgatccaaa tgtagattct
    16861 actgtaaaga ccggtagcga agtaaaactt actgttagtg gtggagaagg taaaataaag
    16921 gttccaaact ttgcagaaat gaatttagat agtgtaaaga aaacattaaa aaatctaggt
    16981 ctagagcttg gtaacgttgg tgaagaatat agtgatagtg ttcctagagg ggaagttatt
    17041 tctcaaagtc ctaatgctaa tgagtctgta gataaaggtt ctaaagtaaa tgttactata
    17101 agtaaaggaa aagaaataaa atcagaaact ataaatatac cagatgtatc aggaaaaagt
    17161 gtagaggaag ctaaatcaat attatctaat gctggtgttg gagtaaatcc tgttaaaggt
    17221 gaggcagcta aaagtgaagg agaagcaggt aaggtttata gtcaaagtca atctggatct
    17281 cttacaatta agcaagggga aaaggtaact ataacaatta attaccacgg tgattatgtg
    17341 aagcctgaaa aatcagttcc aggagtaact ggtaaatctg tagatgaggc taaatcagag
    17401 ttaactaatg cgggctttaa agtaaatgct ataaaaggtg aagcggctaa aagtgcagat
    17461 caagctggaa aggtatatag tcaagatggt ggatcaagtg ctaaagaagg tgcaactata
    17521 acaataactt attatgataa atatgttgag ccaaatcaaa agccagatgg aaataatgat
    17581 ggtactggaa ctggtaatga acaaggcggt caaccttcaa ataatcaaca tccgaataat
    17641 gatactaata aaaaatcata atttttaaat taaaaataaa aagggagtaa atgctctctt
    17701 tttatttttt ataaattgag ataataggct tatttataga taaaaatagg cataagtaga
    17761 aggaagcaag gttatatcat ttttaagctt cttgcatttt atattaaaat ttgtttatta
    17821 taagaatata taatattaaa gatagggagg aaacatggaa ggaattataa taaaaggtat
    17881 aggtggattc tattatataa aaacagatga aggaattatt gaatgtaaag ctagaggtaa
    17941 attcagatat aattcattaa agcctatggt aggagataga gttaccataa aagtagagaa
    18001 tggaaaaggt gtaatagagg atatacatga gagaagctca gagcttataa gaccaactgt
    18061 tgcaaacgta acacaagctt tcgttgtatt tgcaataaaa aatcctgaca taaatttaga
    18121 tttattaaat agatttttaa ctctttgtga gtataatgat atacatgcag ttgtatgttt
    18181 aaataaagaa gatttatgta cagatgagga aaaagaaaac ttaaaagaat taataaatga
    18241 tataggttat gaggttctct tcataaatgc caaggaagga aaaggttttg atgctttaaa
    18301 ggaaagatta gaacataaca taacagtcct ttgtggccct tctggagcag gaaaatctac
    18361 acttttaaat tcttttatag atagagaaca tatggaaaca ggaagtgtaa gtgaaaaaat
    18421 aggaagaggt aagcatacta ctaggcatag cgaacttata gatgtagaca atggatactt
    18481 agttgatact ccaggattta caactttaga tgttactttt atagatagag atagcttaaa
    18541 atattgcttc ccagaattta atgattataa taatctttgc aaatttaatg gatgtaatca
    18601 ttataaagaa cctaagtgtg ctgtaaaaga agcagttgaa gagggtaaaa taaacaagct
    18661 aagatatgaa ttttatataa aaaccttaga agagataata aataggaggg gtaattaatg
    18721 gttaaaatag caccatcaat attatcagca gattttagta ttttaggaga acaagttaag
    18781 agagtagaaa aaggtggggc agagttctta catatagatg taatggatgg tcattttgtt
    18841 ccaaatataa gctttggatt tccaataatg aaagctataa ggaacttaag caatcttgtt
    18901 tttgatgttc acttaatgat agaagagcca ggaagatatg tagatgaatt tataagtgca
    18961 ggagcagatt taataactgt acattatgaa tcagatagac atattgatag aacaataaat
    19021 tacataaaaa gtaaagggtg caaggctgga atagcgttaa atccaggtac accagtttca
    19081 atgcttaaga atttaatacc ttcattagat atggtattaa taatgtctgt taatccagga
    19141 tttggaggac aaagctttat accatatagt ttagataaga taaaagaagt aaaagaaata
    19201 gctaaggctt gtggtaagga agacttatta atccaagttg atggaggagt aggtgaaaat
    19261 aatataaaag agatagtaga agctggagct aatgttttag ttgctggatc agctgtattt
    19321 aaagaagata aaatagaaga aaacattaaa gctttaagag aggcactata aaatgaaagt
    19381 tcttataata gctggaggag aaaagcctag tagtgattta ttaaaaagtg aaattaaaga
    19441 gtgtgactta ttaatagctg ctgatagggg agctgaagcc tatttagaaa atggatttac
    19501 tccagattat gccttaggag actttgattc tataggagaa gaatataaat atgtattaga
    19561 taaaataaaa gtagaaaaat ttaatcctga gaaggataat actgatactg aaattgcttt
    19621 ttttaaagcg gttgaattag gagctacaga aattgttttc ttaggggtta caggaacaag
    19681 attagatcat gttatggcta atttaggact tttaagagaa gctttagaga gaaacattaa
    19741 tgcatgtatt attgataata ataataaaat ttacctaaag aataaaagga caactctaaa
    19801 aaaagaattt ggtaattata tatcatttca agcttttggt tctccagtag ataatttttc
    19861 tataaaaggt tctaaatatg aactttataa tcataaactt ttaataggag attcattatg
    19921 tgtgtctaat gagtttattg atgaatatat tgatatatca tttgaaaaag gtgaagtttt
    19981 agttatatat tcaagagatt aaacacaatt tgagtttctt ctgcataaaa taaaataagg
    20041 aataatttta aaggtaggag agagtttatg agaataatgg ctattaagtt acctaagttc
    20101 ttagctaaaa tagttagaat gttcaaaggt aacaagaaat ctgatacata agatggaaaa
    20161 aattaaggga taagttttgc ttatccctta attttatgta aaataaaaat gcagttatta
    20221 cactgcattt tatccaaaga tttcttatat agctctttga acttttccag atcttaagca
    20281 tcttgtacaa acgtgaacag tttttggtgt tcctttaacg attgctttaa ccttttttat
    20341 gttaggagcc caagttctct ttgattgtct gtgagagtgg ctgtattgta ctccagctac
    20401 aactcctttg ttacaaatct cgcatcttct tgccattaaa aacacctcct ttaaaatgaa
    20461 gataaaatct tcaataggac agataatatt ctatcataat atatattagt tatgcaagta
    20521 taatattaaa ataattcttt aaaaatgtgt ttcaaaaggt atatagttct tgaataatca
    20581 aagtatttaa tatagaatat agtatatgta ataggtttgt gtgtttattt taagtgactt
    20641 ttataggaat ttaatataaa atgcagtata tctaatagtt gaggaggaat cgccatgata
    20701 ggatattcaa atgaatatgg aaatgtgtct tatactgatg acgttttagc aaagattgtt
    20761 ggattatcaa caatggaatg ctacggagtt gttggaatgg tttctaagag cgctacagaa
    20821 ggcctttggg agttaatgag aatagaaaat ttaacaaaag gcgtaaaaat agatgataaa
    20881 aatgataagt taacaataga agtatttgtt atggtagaat atggtacgaa aatatctgta
    20941 atagcaaaca atataattca aaaagttaaa tataatgttg aaaattatac tgggcttaaa
    21001 gtatctagca taacagtaaa tgttcaagcg gttagagtct aggaggtaaa acagaatgaa
    21061 atttaataaa gtaaatgggg aacacttgta ttatatgatg agtaatgcag caaataagct
    21121 agagttacag agtgaatatg taaactctct taacgttttc ccagttccag atggagatac
    21181 aggaacaaac atgtctatga catttagagc tgctgttaag gaaatcgaag gaatggataa
    21241 caaaaacatt ggtgaagttt ctaagaaact agctaagggt gctctaatgg gggcaagagg
    21301 aaactcaggt gttattcttt cacaaattct tagaggtata tcaaaagggc ttgaaggaaa
    21361 agaagaggct gatgcaagtg aatttgctaa tgcactttta gagggaagta aatcagcgta
    21421 taaggctgtt atgagaccaa cagagggaac aatacttaca attgttagaa ttgctggtga
    21481 agtggctgta gccttaaaag aagataatat aactgaactt atgagagaag tatgtagaga
    21541 aagtaaaatt atgcttgata agacaccaga aatgttacca gcattaaaga aagctaaagt
    21601 tgttgattca ggcggaatgg gattattaat aatccttcaa ggaatgcaag aagctttaga
    21661 aaacggatta aaagtaacta caggtacacc acaagctgtt aaatcttcag cagttaaggc
    21721 tcaaagaagt gaaactatga gcgaagaaga tattaaattt ggatactgta ctgagtttat
    21781 aatattagga gactcaaacc atgcagaaga atttaagtca aaagttataa ataagggtga
    21841 ttctttagtt gttgttggat atgaggatgt aattaaagtt catattcaca caaataatcc
    21901 aggaaaagtt ctagaagagg ctgttaaatt tggagaactt tcaaaaatta agattgataa
    21961 catgagagaa gagcatagag aaatgcttga aggtatgtat gaagaggcag ctgaaacaga
    22021 agagagtgtt gcagctggag agtttaaaaa atatgcattt atatcagtag caatgggtga
    22081 aggtatgaaa aacatattta aagacttagg tgttgactat gttatagaag gtggtcaaac
    22141 tatgaaccca agtactcaag atatgttaga agctattgaa aaattaaatg ctgagcatat
    22201 atttatttta cctaacaata aaaatataat tatggcagct aatcaagcag ctgaaatatc
    22261 agataaggat ataagagtta taccaactaa gacaattcct caaggaataa cttgtataac
    22321 tatgtttaac ccagaagctg atgttgaaga aaatactgaa gaattaaaag aagctatgga
    22381 aatggtaaaa acaacttctg taacatatgc agttagagat actgaggttg atggaaaaga
    22441 aatcaaagaa ggtaatattt taggcttagt tgaaggtaag ataaaagaag ttggagaaga
    22501 tccatataaa gtagctgagg atttaataga ttctttagtt gatgaagatt cagagttaat
    22561 aactatattc tatggtaaag attgtgaaga ggaaaaagtt gatgccttaa ttgaaaaact
    22621 agaaggaaaa tatgaagatt tagatgttca atgttataaa ggcgaacaac cattatatta
    22681 cttcataatg tcagtagaat aattaagagt taataatttt tagtgaaatt ttagtttttt
    22741 aaagctaata tgaatagaaa ttttattata gggttatagc tataagtttt atggttataa
    22801 tcctttaatt ttgcaatttt aagaatataa ttaaggtata tttatttttt atagatgatt
    22861 aaatagcttt attattaagg aggggattag gattaacata tacgatgata tagggttagt
    22921 aaaaggtgtt ggacctaaac ttaaagagag acttaataaa gttggtatat ttactgtttt
    22981 agatttactt ttatattttc caagggacta tgaattttta aatgatgata taagtttaaa
    23041 tggagataca agtgatgaaa aagctatttt aaaatgtaaa gtcaaaagtt atggaagttc
    23101 cataaaaact agaaatggaa aaactttaac cacaattaac tttacatata ataatttaaa
    23161 ggttataggg aaatggttta atcagcctta tataaaaagg aatttcattt taggtaatga
    23221 atataatctt atggggaaat ttaaaaaggt caataatact ctagaggtta ttaatccatt
    23281 aataccttgt aaagaagcta ataaaagtga gattttacct atatacactt tgaaaaatgg
    23341 attaacaaac aaaattttag ttaaacttat aaatgaaata cttaagaata tgattataaa
    23401 agaaaattta ccagaggaaa tagtgaaaaa gtataaactt atatccttag ataaagctat
    23461 aagaaacata cattttccag tgggaagagg agagttacaa agtgcaataa atagacttaa
    23521 gtttcaagaa ttattcacat actccctaaa aataataatg atgaaagctc atataaagaa
    23581 agaaaatagc ggaataagtt ttaaaatgag tcctcttctt aaggacttaa aagagtcact
    23641 tccatatact ttaactaatg cacaatcaag aactcttaga gaaatacttt tagatcaaaa
    23701 gagaaatatt gccatgaata gattggttca aggggatgtt ggatctggaa aaactttagt
    23761 agcattaata tctatgttta atgtgtacat gaacggatat caaactgttc ttatggcacc
    23821 tacagaaata ttagctaatc aacattatgc tgaagctaaa aaatatttag ataaatttgg
    23881 agttgatata gaacttttaa ctggaagcac taaggaaaaa gagaagaaga gaataaaaga
    23941 gaaaatagct tcaggtaaag aaataatgtt aataggaact catgccttaa tacaggatga
    24001 tgttgaacta aataatttag gtcttgtggt aacagatgag caacatagat ttggagtaga
    24061 acagagaagt aggcttataa ataaaaataa gagagcggat gttttagtaa tgactgctac
    24121 ccctattcca agaactcttt ccttatattt atatagtgac ttggatattt ctataataga
    24181 tgaattacct ccaggaagaa agccaataga tactatgcta gtagatatga atcaaagaat
    24241 gaaggcttat aactttgctt taaatgaagt agagaaagga agacaattct atatagtatc
    24301 acctttaata gaggaaaatg aaaaattaaa tctaaactct gttgaaaaaa tttatgaaga
    24361 gcttaaaaat ggaatcttta aggatgtaag aattgaaatt ttacacggta agatggctgg
    24421 aaaagataag gataaaatta taaatacctt taaaaatggg gaaataaaag gtataatatc
    24481 tacaacggtt atagaagtag gtgttaatgt accaaactct acaatgatga tcatagaaaa
    24541 tgcagagagg tttggtttag ctcaacttca tcagcttaga ggaagagttg gaagaggaga
    24601 acataagtct tattgtattc ttatagctaa tactaaaaat gatattacta gaagaaggat
    24661 gaaaataatg acagaaagtt cagatggttt ttatattgca gaagaggatt taaaactaag
    24721 aggtgctggt gaagtctttg gaatgagaca gcatggagat gaaggattta ttctagctaa
    24781 tgtagttgat gatataaata tattaaaatg tgctaatcat gaagctaaat taatagtaaa
    24841 taatataaat gaagataata aaaagctttg tacagaaata atgagaggaa ttgaaagaaa
    24901 tagtaggtat atttgtttca attagtaaat tgttcaaaaa ctagtaatat gataaaatga
    24961 ttaaggaaaa atcaaaggag gacattataa atgagaataa tagcaggaaa agcaagagga
    25021 agaaaactat tatcaccacc aacatatgaa acaagaccaa cactagatag agtaaaagaa
    25081 tcaatgttta gtatgattca atggtatata cctgatgctg tagttataga tgtttttgca
    25141 ggaacaggaa gtttagggtt agaagcagca agtagaggag ctaaagaatg ctacttagtt
    25201 gataagagtc cagtaacatt ccctgtttta aagaaaaata tagaaaattt aggcttcgga
    25261 aatttttgtc atgccctaaa cacagatgct tattctgcat taagaagttt agcttcaaga
    25321 ggtaaagtat ttgatttaat gtttatagat cctccatata tgaaaaatct tattccagaa
    25381 gctatagaga taattgaaga aaagaactta cttcaagagg atggattaat agttactaaa
    25441 atagattcaa gtgaagaaat ttttgaggga actgaaaaga taaaacttac aaaagttaag
    25501 aagtatggaa atacaacagt ttgtttttat aaaatagagg aggactaaaa tgagagtagg
    25561 ggtttatcca ggaagttttg accctattac taaaggacat ctagatctta ttgaaagagc
    25621 ggcaagtaaa tttgataagg tcatagttgc tgttcttata aacataaata aaaaaggtat
    25681 gttttctata gaagaaaggg ttaatcttat agagaagtgc gttgcaaaat acaataatgt
    25741 tgaggttaaa agttttaatg gacttttaat tgattttgtg agaaaagaaa aagctgatgt
    25801 aataataaaa ggtttaagat cagttactga ttttgaatat gaattccaaa tggcacttat
    25861 gaacagagag ttagcaaatg aggttgaaac tgtatttatg gttacaagcc ctaattactc
    25921 ttatataagc tcgtctgcaa taaaacaggt tgcaagtttt aatggagaaa taaaaaactt
    25981 tgttccaaaa gagatagtag aggatttaga agaaaggatt attagtttaa gaggggaggg
    26041 ataattatta acatggagtc acaagttatt ggaattttag attacttaag agatattgtt
    26101 gagggagctt ctaaggtccc tataacaggt aaggttgttg ttgatagaaa agaagtttta
    26161 gaacttttag accaagtgat taattgtctt ccggatgaga ttaaaaaagc acagtgggtt
    26221 ttaggagaga aagaaagaat cttagctgaa gctagaaaag aaaatgagag tgttaaacaa
    26281 gaaacttatg aaatgatgaa aaaaagaatt gagaatcata atatagtaaa agaagcagaa
    26341 ttaagagccc aagagataat agctaatgct caaaggcaag caaaaactat tagactaggc
    26401 tcaagagaat atgctgatga aatattatct caattagaaa ttgaaataac taataaaaga
    26461 gaagaatttc ttaatcatat gaaaaataat atggagaagt ttgctattga tttcacagaa
    26521 gacttagata aagcaactgg tgaaataaga aacaatatta aggaattaag aggcgacaag
    26581 taatttatat aaaattgcta agccaagggt tattcctata attaatatta ttggagaaag
    26641 tgtaattaaa aatgagcttt ctataagtga aactgcaata ttatttttaa atataatata
    26701 aacgaaaaac ataagtacaa aggcaattat tccttgcaaa actttaaata aaaagtattt
    26761 tattatagat acattttctt tataaaagaa agaagaagtt tgcattatta ttgaaagtcc
    26821 accaaaagca cttaaaaatg atattaagag taatttgcta aaaagtgata aattactaga
    26881 agctattatg ttacatccat ttgtaacttc tatgcttcct aaaaataaac cgttaaatat
    26941 atcaggaggt atatttaata tagaacttaa gtttgttaat gttccagttg aaataaataa
    27001 ggtttttaac atacttatta ttactgaaaa cataattaca tatccgcata ggataagtat
    27061 atttaaggaa gcatcctcaa tagacttttt aatcacttct cctatgttaa aagtagattt
    27121 agtttcagta atgacttctt tagaaagaga gagttttttc tctctttttt tatttttagt
    27181 tattataccc ataataattg cagaaagata acttggtatt aacaataagt atccaagggt
    27241 tgaattacca agcatagatg ttcccacagc acctaataga aataaagggc ctatattaga
    27301 agctatattt aaaagtctta caaattcatc gtggggaata agttcctttt tatataagtc
    27361 agtagaatat tttgcaccaa gtggatatcc acaaagaaaa cttgcaacta aagcaaagct
    27421 acaaggataa cttaatccta atggcttaca tagaatagga cctaatatct ttgaatatag
    27481 ttttattcca tctaaattaa ttatcatgtt acatataacc ataaatggaa acatagttgg
    27541 aagcacagag taaaaaaata actttgcacc agctaaggat gcttccatgg aaagtttagg
    27601 atttactata aagtaaatta ctgttagggt taaaaataaa gaaaaaataa tattgccttt
    27661 tatttttaat aattttatta aagttatact tagtattatt ataagtaaaa ataaaatact
    27721 atacatggtt tcctcctttt acgaaatcat atatagtatt tatatttaag tacatataat
    27781 aatattattg ttatctaaaa ataataggac taattaaata atcactgtta attttaactg
    27841 atggatttag aaagctataa atattagtgg cttttatgtc aaattctaac aaaggatctg
    27901 ttttagcttt tgataattta ttaacgatat ttatttcaga atttttctta atctctttta
    27961 aaatttccct tcctttatta ttaaatccta agattcttac ataattagga gaatattttc
    28021 taagagaaag aagatcttta tttacattta atagtatgtg acataaaatt ctgtttaatc
    28081 tagtatatga ataacgctta cttttgcata ggtttattaa ttcatctaaa gaattagctt
    28141 ttcctatgtt ttgaattatc ttattatcta ttccttcaga agcctctggt atttctttta
    28201 atatattagg attgcttact atagcgtatt ttactaagtc aaacattttg tctaaatcac
    28261 taaaagaagt attttttaat aagttatagc tatactcagg cataaaatca aaactttctt
    28321 ctatatttga agtgtaaata atttttctta tggcagaagc agaggctatt tcattttttg
    28381 aaagttcttc atcattgtaa tctgctccta atctttgtat agtaaaaggt tttatagtac
    28441 tttgaagctt ataaaggctt ttacaatatt caatggctaa tatgttatta gaagagttta
    28501 gaactttttc taagcatgaa aaatcagttt tataattatt ataatctaag tacttcatta
    28561 aagcttcact tctagcttta ggaaagggaa ggccttcctt taaataatcc tttaaatatt
    28621 ctttgaatat aggtggttca ttgacaataa tttcactaag ttttttaatt aaatctatat
    28681 ctccacattc tgatccaaaa caaatattat taactacatt taaagaatta agcaaagata
    28741 ctgcaccaaa ggcaaaaaat tctgctgaag aagttgcaaa aagagttgga agttctacaa
    28801 ctaaatcaac tccagcttct aaggccattt ttgttctagt ccatttgtct gttaaagcag
    28861 gcaaacctct ttgcacaaag tttccactca taacacaaat aactccatcg caatttgtaa
    28921 tctcttttga attttttaga tgaagttcat gacctagatg aaaagggtta tattcagtta
    28981 ttatgccagt tatattcatt taaatcacca ctttttccta ttttgataaa actataataa
    29041 cct
//
LOCUS       DAEVGI010000016        27915 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEVGI010000016 DAEVGI010000000
VERSION     DAEVGI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 27915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27915
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.016"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(117..863)
                     /locus_tag="K8M77_000674"
     CDS             complement(117..863)
                     /locus_tag="K8M77_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI7085190.1"
                     /translation="MEKKLLGKIVYITGASKGIGRGIAREFGKAGAFVLVGYNSDLEG
                     ANETLNMIKELGGSGEILGGNVANKDERNNIVSSIKNKFGKLDVLVNNAGISKIGLFM
                     DASDEEIDSIMNVNLIGAMKLTRDSMDLLREGSNSSIINISSIWGNVGASCEVLYSTT
                     KGGMNLFTKALAKEIAPWGIRVNCIAPGVINTEMNSWLSKEEKEGLEEEIPMNRFGEV
                     EEIGRVAKFLASEDSSYLTGQILTVDGGML"
     gene            complement(969..1931)
                     /locus_tag="K8M77_000675"
     CDS             complement(969..1931)
                     /locus_tag="K8M77_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBI7085191.1"
                     /translation="MKIFMKFIDFHCDTASRMLQENKKLFRNDLKVDINKLREGEALA
                     QFFALFINKECNFDTYSYCKEMLQNFKREINENSRDIVLCRNISDLEKAEKDGKIGAF
                     ITIEEGDAIKGDIEKLREFKEEGVSLITLTWNYINDLGYPNYEFKYKDKGLTKKGIEV
                     VEEMNNLGMLIDVSHLSDGGFYDAIKYSKSPIIASHSNSRTKTNHSRNLTDHMIKELA
                     NNGGVTGINFCNAFLKEEWEEDLNLASIRNMVRHIKHIRNIGGIDVISLGSDFDGIEN
                     EVEIKDSSKMNLLLNALEIEGFKAEEIEKIYYKNAERIIRDVLR"
     gene            complement(2181..3413)
                     /locus_tag="K8M77_000676"
     CDS             complement(2181..3413)
                     /locus_tag="K8M77_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBI7085192.1"
                     /translation="MNFDNLEREDEQIAHLVQKEKERQENSIELIASENFVSKAVMEA
                     MGSYLTNKYAEGYPSKRYYGGCHVVDEVEDLARERVKKLFGAEHANVQPHSGSQANMA
                     VYFSILESGDTVLGMDLSHGGHLTHGSPVNFSGRLFNFVSYGVDKETETINYETVREL
                     ALKHKPKLIVAGASAYSRIIDFKTLREIADEVGAYLMVDIAHIAGLVATGLHPSPVPY
                     ADFVTSTTHKTLRGPRGGLILCKEKFAKVLDKNIFPGIQGGPLMHIIAAKAVCFKEAL
                     EPSFKTYMEQVVKNAHVLAEALESYGFKLVSNGTDNHLILVDLTNKDITGKDAEILLD
                     SIGITLNKNTVPNETRSPFVTSGVRIGTPAITTRGFKEEEMKEIASIINDAIKEKDGD
                     LEPLKARVKALCAKYPLY"
     gene            3593..4363
                     /locus_tag="K8M77_000677"
     CDS             3593..4363
                     /locus_tag="K8M77_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBI7085193.1"
                     /translation="MDFDKVLHIATFAGRIILESGGETYRVEETICRICAAYGVRYAE
                     SFVTPTGIMVSVYDENNYTTTLVKRVTNRTVDLQKIHLVNELSRKLYTEKYSLDEVLE
                     KLIDINENCPKYNFRTNLVFSAITAAGFTTILGGNIEDCVVSFFVGMFVKLATFKAGE
                     LGINAFFINTLGGAIAALLSLIAYSLGVGSNLDTMIIGSIMLLVPGLSITNAIRDTIA
                     GDLISGLIRAAEAFLVAISIAIGTGMIMSIWIYFGGGI"
     gene            4364..4885
                     /locus_tag="K8M77_000678"
     CDS             4364..4885
                     /locus_tag="K8M77_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBI7085194.1"
                     /translation="MVLIKEVLATFIATIAFGVLFNIHGKNLFFAGIGGALGWLVYKI
                     GLYFGLSVILSMFFSAIAFSAYSEIYARILKTPVTTIVVCALIPLVPGGGMYNTMYEA
                     IKGDAMSTWNQAIMTISSSGALALGVLFVSTITRLINNRKKQYTHGSLNPKNILLFKK
                     LKNKSNIDFHTKK"
     gene            complement(4903..5175)
                     /locus_tag="K8M77_000679"
     CDS             complement(4903..5175)
                     /locus_tag="K8M77_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBI7085195.1"
                     /translation="MNDNEALRKDILVRLRKIEGQVKGIQGMVEKDVCCRDVLTQISA
                     IRAAINKVGVLLIENYARNCMGIGEEDKEELAGLEQLIKTLNSFVK"
     gene            complement(5461..7254)
                     /gene="aspS"
                     /locus_tag="K8M77_000680"
     CDS             complement(5461..7254)
                     /gene="aspS"
                     /locus_tag="K8M77_000680"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBI7085196.1"
                     /translation="MGEALNGLKRNIMCGDARESHIGQKVTVMGWVQRNRNLGGLQFI
                     DLRDREGILQVVFNDDLGEEILEKAKSIRPEYCIAVTGEIVKRESVNPNMPTGMVELK
                     AEELKILSESDTPPIYIKEDLDAAESIRLKYRYLDLRRPDMQNIFKIRHKTTKAIRDY
                     LDQNGFLEMETPILTKSTPEGARDYLVPSRNYPGMFYALPQSPQLFKQLLMVSGFDRY
                     FQIVKCFRDEDLRANRQPEFTQVDLEMSFVEQDDVMALNEGLIKHVFKEVLGVDVKTP
                     IKRMTFKDAMEKYGSDKPDLRFGMEITNLSDVVKECGFKVFTDAVANGGSVRGLCLEG
                     GASMGRKDIDRLGEFVKTFKAKGLAWIQLKEEGVKSPIAKFFSEEELNKIIETMGAKT
                     GDLILIVADKNSVVLKALGELRLELSRKFDLVKDKSEFNFTWITEFDLLEYDEEEGRY
                     FAAHHPFTMPMDEDIKYLDTDPGRVRAKAYDLVLNGEELGGGSIRIHDTKLQEKMFEV
                     LGFTQESAWERFGFLLEAFKFGPPPHGGLAFGLDRMIMFLAGTENIKDVITFPKNQNA
                     FCYLTEAPNIVDEEQLKELGIETIKKEDTAE"
     gene            complement(7275..8522)
                     /locus_tag="K8M77_000681"
     CDS             complement(7275..8522)
                     /locus_tag="K8M77_000681"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBI7085197.1"
                     /translation="MAIQAQKGTKDMLPNDAYKWHYIEEKLRKISAEYGIREIRTPMF
                     EATELFKRGVGETTDVVQKEMYTFEDKGGRSITLKPEGTAPAVRAFIENSLYADAQPT
                     KMFYFTPCFRYEKMQKGRLREFHQYGIEVFGSQEASIDAEILSLVMRALTEDFGIKGL
                     SLNINSLGCPKCRAKFNEALKQYLKENYDNLCETCKTRFEKNPMRIIDCKEKRCKEIV
                     KEAPSILDYICEECSDHFSKLKAYLDVMGIEYNIDPQIVRGLDYYSKTVFEVIKDGLT
                     VCGGGRYDYLVEEVDGPKTPAMGFGLGLERLLLILDEEGIEIPEPVRCEVYIGSMGDR
                     AKLEAMKLAFNLRKSGIKAEIDHLGKSVKAQMKYANKIGAKYTFVIGDSEIEENKIKI
                     KRMSDGEQFEVSLDINEIVNIVK"
     gene            complement(8554..9996)
                     /locus_tag="K8M77_000682"
     CDS             complement(8554..9996)
                     /locus_tag="K8M77_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBI7085198.1"
                     /translation="MYIKIYLNDLKYRYDVYQMFNIFYTFKELKFVNEDEERDYDVFI
                     SENMVKISEGDNSFSYEFKEGYGFKTELKKGIFKFLSETLKDEYPWGTLVGIRPSKIA
                     LSLIREGKSEEEIIKYFEDNYMAREEKAKLCIEVAEREESFVNKEEKNISIYVGMPFC
                     PTRCLYCSFAANPIAGCKKDVEPYLEALSKEISAISDYVSKKGLKIETVYFGGGTPTS
                     VNNEQFEVLMKHIYDSFVNNKGIKEFTVECGRPDSITEEKLKTMKRYEVSRISINPQS
                     MNDKTLKSIGRGHLTEDVVDKFNLARSLDFDNINMDIIIGLPNEDISEVSKTCSMIKE
                     LNPDSLTIHGMSIKRASRLHENLVLHNTITIAEQKNLNKMYEMSKVLGRELNMHPYYM
                     YRQKNMVGNMENVGYSKDNKECIYNIQMIEDKQTIIALGADAVSKVVFLEEDKNRIER
                     FANVKDVKEYVKRIEEMVEGKIELLDTLYK"
     gene            complement(9986..10606)
                     /locus_tag="K8M77_000683"
     CDS             complement(9986..10606)
                     /locus_tag="K8M77_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI7085199.1"
                     /translation="MLIKTIPVGMYQANCYVVIDEETKDCIIVDPGEEASRIENVIES
                     LDAKPKMILLTHGHFDHVGAVQKLAEKYKIPFYIDKKDEDMIERNVDVFGKLPKADGY
                     LKDGDTLKFSDNYEIKCLETPGHTPGGLCFLIDNCLFTGDTLFRESIGRSDFEGGNHA
                     TLLKSINDKLVPLGDDIVVYPGHGPSSTIKHERQRNPFLAGDFYVY"
     gene            complement(10766..11215)
                     /locus_tag="K8M77_000684"
     CDS             complement(10766..11215)
                     /locus_tag="K8M77_000684"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBI7085200.1"
                     /translation="MRVVVQRVNKSSVKVDNEIVGSINKGFNVLVGIGKEDTIEDLKY
                     MKDKVLNLRVFEDEEDKMNLSLKDVCGELLLISQFTLYGDCRKGRRPNFMNALGGDEA
                     KKLFDEFVSMCREEGIKVETGVFGAHMVVDIENDGPVTLILDSKKNF"
     gene            complement(11230..13410)
                     /locus_tag="K8M77_000685"
     CDS             complement(11230..13410)
                     /locus_tag="K8M77_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBI7085201.1"
                     /translation="MLEELISKIKANGNNVDIDLVKKAYDLAFEAHKEQKRESGEPYI
                     THPISVAMILADMGMDTNTIVAGLLHDVIEDTDYTYEDISNIFNVEVANLVDGVTKLG
                     KIKYKSKEEQQADNVRKMLLAMAKDIRVIIIKLADRLHNMRTLKYMKPEKQKKKAQET
                     LDIFAPLAHRLGISKIKWELEDLCLRYIHPEEYYDLVNMIAEKRVEREKFISRIIKEL
                     KENLDKANIDSDIEGRPKHFYSIYRKMVNKHKSIEQIFDLTAIRILVNTVKDCYAVLG
                     IVHTIYKPIPGRFKDYIAMPKPNMYQSLHTTVIGSEGKTFEIQIRTFEMHRTAEYGIA
                     AHWKYKSGINGTDSKDMTFENKLTWLRDILEWQKEAVDATEFMEGFKLDLFSDEIFVF
                     TPKGVVINLPVGATPLDFAYKIHTDIGNKCVGAKVNGKIVTLDYKLKTGEIVEILTSS
                     SSRGPNIDWLNIANSNQARSKIKQWLRKARREENLERGKEMLDKECKKQSLVFSDLCK
                     GPLYDKLLKRYHLNNVEEIYVAIGEGELLSSTVISKLKENIVKQVAEEELNKNIEEQI
                     AKTERQIKKKQNYGVTVKGLNNIMVRFARCCNPVPGDDIAGYITKGRGVSVHRKDCSN
                     FKAIVEKQEEKVVDVSWGTEKGAAYVAELEVKAEDRMCLLSDVMLVITDSNFRLLSLN
                     AKSGRNGVANINIQVKIDNIEQLKELMKKIRRLQGILDVYRVNK"
     gene            complement(13562..14080)
                     /locus_tag="K8M77_000686"
     CDS             complement(13562..14080)
                     /locus_tag="K8M77_000686"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBI7085202.1"
                     /translation="MSLKDKIRVIEDFPKKGISFKDITTLIADGEGLRDSVDQMAEFF
                     KDKNIDVVVGPEARGFIFGVPVAYALGVGFIPVRKPGKLPGDTVRVEYDLEYGKDALE
                     IHKDAIKPGMRVAIVDDLLATGGTIAAVAKLVEQAGGEVAGLAFTIELTELKGRDKLK
                     GYEVTSLVDYDV"
     gene            complement(14125..14979)
                     /locus_tag="K8M77_000687"
     CDS             complement(14125..14979)
                     /locus_tag="K8M77_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HBI7085203.1"
                     /translation="MQKAWKNIYCTNYISGYSPFNIKNMNKAIERLASAINNREKIVI
                     YGGCDLDSICAISSLMLILKYLNADVEYCVSSEHEEKNYDIDEYTLREDIAFLGAELL
                     ITVGCGLKNKEVENLCNRFNIDLIIMENKRTKCIYDSTYINPSEEKCSYRYKDLTNSG
                     LTFKLMQAIAIYYNMKRINKYLDLILIGSVSKNVKNKGENGIILKEGIQYLRYTNSIG
                     LNAIKSFFNISDINIDSINKIVNEITPREGAISKKDNAKIVVELLTTNDKDRAEQITK
                     YIYNEKIR"
     gene            complement(15179..16048)
                     /gene="secF"
                     /locus_tag="K8M77_000688"
     CDS             complement(15179..16048)
                     /gene="secF"
                     /locus_tag="K8M77_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBI7085204.1"
                     /translation="MLKIVEKKKIWFTISILIIVIGFGFMFTKGMNFGIDFRGGTQVV
                     INMGTDFNKTDVDKIVEEYSKDAVTNQVNGTELQIKAKELNSSQVNDIFKALKEKYDL
                     KDDALVSQNEIGASVGRDLTTNAIVALAIAVIGMLIYVIIRFEFSFGIAAIIALLHDV
                     LITISVYAIFGITINTPFIAAILTIVGYSINDTIVIFDRIRENRKKLRGKSVEEIADM
                     SITQTMSRSINTTLTTLITIVCVYIFVPTVRDFAFPLIVGIASGACSSIFIASPIWCI
                     LANRQKNKKAINK"
     gene            complement(16041..17300)
                     /gene="secD"
                     /locus_tag="K8M77_000689"
     CDS             complement(16041..17300)
                     /gene="secD"
                     /locus_tag="K8M77_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBI7085205.1"
                     /translation="MKSKLGSFILFFISIAVISFLALAGFKGFTLGDYQFKTFNEVIT
                     KGLDLQGGVSVLMEIQSNDVTVDQLNTTKELLSLRVNKVGVSETVVTTEGNNRIRIDI
                     PGQYDSKTIVDSLQKTGELTFVGPEDDVILTGKDVEKSSVYIDPQTGKPVIKLELNEE
                     GKQKFAEATKKYKGQKIAIKMDNETLTDPVVNDIISNGEAIISGNRSMEEAEKVSGII
                     NAGALPVPVKAVSVETVGAQLGANALPNALKAGAIGVAIIFLFMILYYRVPGFIACMS
                     LSVYILLVLYIFALVGVTLTLPGIAAFLLTVGMAVDANVLIFERIKEELGNGRSITSA
                     MKIGFSNALRSIMDSNITTLIAGLVLYFFGSGPVKGFALTLLIGIVISMFTAIIMTRF
                     FMNLGFNMGILNKPSMFGRIKGGRHNA"
     gene            complement(17398..18771)
                     /gene="scfB"
                     /locus_tag="K8M77_000690"
     CDS             complement(17398..18771)
                     /gene="scfB"
                     /locus_tag="K8M77_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBI7085206.1"
                     /translation="MALIHKFMQGGEYYVVDVNSGSVHIVDDLIYNMIDNDNKLSSKE
                     SLIEKLKDKYPVEEIEEAYEDLLQLVEEDALYSGDLYEEVAKESDKAPSYIKALCLNV
                     VHDCNLRCKYCFADEGEYKGCRKPMSAEVGKKAIDFVLANSGGIKNIEVDLFGGEPLM
                     VFDTIKEIIDYGKKRGQEVGKNVRFTMTTNATLLNDERIDYIDKNIGNIILSIDGRKE
                     VNDAVRIRVDGSGSYDRILPNIKKMVEKRDPSKQYYARGTFTRNNTDFFQDVMALANE
                     GFTEISIEPVVLPDEHELSLRREDLPTIFGEYDKLYNEMVRRLEEGDNIFKFYHFNID
                     LNGGPCVYKRIAGCGAGHEYIAVTPDGEIYPCHQFVGNTDFLLGNIYDGEIRKDLSTE
                     FKQAHIYNKPKCKECWARFYCSGGCQANNFNFNGDIHVPYEVGCEMQKKRIECAIALK
                     SKTMDAE"
     gene            complement(18841..18981)
                     /gene="scfA"
                     /locus_tag="K8M77_000691"
     CDS             complement(18841..18981)
                     /gene="scfA"
                     /locus_tag="K8M77_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBI7085207.1"
                     /translation="MKRIKTVNKSSLKKSLCKPADCRECANSCQSACKTSATVANLAC
                     EN"
     gene            complement(19176..19445)
                     /gene="yajC"
                     /locus_tag="K8M77_000692"
     CDS             complement(19176..19445)
                     /gene="yajC"
                     /locus_tag="K8M77_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBI7085208.1"
                     /translation="MNFQQIATMVLPFILMFGVFYFLLILPEKKRKKKYDAMIDELKV
                     NDKIITRGGIIGRIVKLKDDSVIIETTQDRTKIEFSKQGISSKID"
     gene            complement(19484..20626)
                     /gene="tgt"
                     /locus_tag="K8M77_000693"
     CDS             complement(19484..20626)
                     /gene="tgt"
                     /locus_tag="K8M77_000693"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBI7085209.1"
                     /translation="MTKKRYTLLKKDGKARRGEFVTPHGTIQTPVFMNVGTLAAIKGA
                     VSSMDLKEIGCQVELSNTYHLHLRPGDKIVKQMGGLHNFMNWDRPILTDSGGFQVFSL
                     AGMRKIKEEGVYFNSHIDGRKIFMGPEESMQIQSNLGSTIAMAFDECIPNPSTREYVE
                     KSVARTTRWLERCKKEMDRLNSLDDTVNKEQMLFGINQGGVYEDIRIEHAKTIREMDL
                     DGYAIGGLAVGETHEEMYRVIDAVVPHLPEDKPIYLMGVGLPSNILEAVERGVDFFDC
                     VLPARNGRHGHVFTKEGKINLMNAKFELDARPIDEGCQCPACKNYTRAYIRHLFKAKE
                     MLAMRLCVLHNLYFYNKLMEDIRDAIDGGYFAEFKAKKLEEWNGRA"
     gene            complement(20650..21675)
                     /gene="queA"
                     /locus_tag="K8M77_000694"
     CDS             complement(20650..21675)
                     /gene="queA"
                     /locus_tag="K8M77_000694"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBI7085210.1"
                     /translation="MKVSDFYFELPEELIAQYPLEKRDSSRLMVLDKKTGEIEHRKFH
                     DILEYLNEGDTLVLNNTRVLPARLIGEKEETGGKIEFLLLKRIEGDKWECLAKPGRKA
                     KVGTVFTFGEGKLKAIVREIGEEGNRIIEFKYDGIFEQVLDELGQMPLPPYIHEKLED
                     KERYQTVYSKEKGSAAAPTAGLHFTEDLLKEIKDKGVNIAYLTLHVGLGTFRPVKVDD
                     VNNHVMHSEYYHLDKENAELINKTKEAGKRVIAVGTTSSRTLETIGDENGRVREQSGW
                     TDIFIYPGYKFKIVDNLITNFHLPESTLIMLVSALAGQDNIMNAYNTAVKEKYRFFSF
                     GDSMFIK"
     gene            complement(21697..22737)
                     /gene="ruvB"
                     /locus_tag="K8M77_000695"
     CDS             complement(21697..22737)
                     /gene="ruvB"
                     /locus_tag="K8M77_000695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBI7085211.1"
                     /translation="MSERLVTSNEIGIDSTNEYSLRPEKINEYIGQDKVKERLNIFIK
                     AAQRRGEALDHVILYGPPGLGKTTLANIIANEMGGNLKITSGPAIERAGDLAAILTTL
                     NTNDVLFIDEIHRLNRSVEEILYPAMEDYVLDIIIGKGAASKSIRLDLPKFTLIGATT
                     RIGMLSSPLRDRFGVLCSMEYYTDEQLKEIIIRSAEILGCHITEEGAFEIAKRSRGTP
                     RIANRLLKRVRDFAEVLYDNEITEEAAKKSLEILEVDGEGFDRIDNKILEAIIDNFNG
                     GPVGIETLAYFVGEELDTIEDVYEPYLLQKGFIVRTPRGRMATDKAYKHLGRVRFNES
                     KIDSKQCTLFEK"
     gene            complement(22751..23356)
                     /gene="ruvA"
                     /locus_tag="K8M77_000696"
     CDS             complement(22751..23356)
                     /gene="ruvA"
                     /locus_tag="K8M77_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBI7085212.1"
                     /translation="MYEYIRGQFQGISKDYVVIELNNIGYRIFTSGNTMSNMPKVGDE
                     VLLYLEQIVREDFIGLYGFTTKEELEMFKLLLSINGVGAKAALSLLSISTVNNLKYAI
                     MMGDEKHITRAPGIGKKTAQRIILELKDKLKPDELTSEEEQLIEGINDNSDYSFNINE
                     TLSALMALGYTEKEAQKALEKVDKTLSIENMIKESLKLLMR"
     gene            complement(23584..24177)
                     /locus_tag="K8M77_000697"
     CDS             complement(23584..24177)
                     /locus_tag="K8M77_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085213.1"
                     /translation="MNFNNIKNNDKIKNLIFILLSIFIFASSYMITYFYNSRKIQQTN
                     MKVTEKKGELVLAENMDVIFTRRTLDDHVVVDYKTTIGEMVKNDEINGENMEELISAV
                     EKDGYKLVSSTSGEIIFLNDMGILEAGKYYIGEKDGYIAIFKAGEDGRPFIENPEDVS
                     TKKIEDLPEVDRSKISNFEKKYDTREECEENITNYIS"
     gene            complement(24324..27851)
                     /locus_tag="K8M77_000698"
     CDS             complement(24324..27851)
                     /locus_tag="K8M77_000698"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase/5'-nucleotidase"
                     /protein_id="HBI7085214.1"
                     /translation="MLKKVRDKKFTALAVATSMIFGITTPLTTVKATENLAPENLEKK
                     GEEASLKKEGDVTIQILGTSDLHGRFMNYEYARNQKSDGGLNQISTLVNEARKENPNT
                     IVVDNGDTIQGNFNHLFKNGPNPMVMGMNAIGYDVFSLGNHEFNYGMDNLNNVVSQAN
                     DNLDVLCANLYKDGKRVYEGYTTREIEGVEVALIGVVSPHIMKWDSENLKGYEAKNPA
                     EEVGFVIDEIENRPEGGADVYAVVSHVGLESEYGNGDSARAIAEMNPEVSAIVAGHSH
                     TNVPEEKINNAVISQPTSNGQMVSKIELTVRKNGEKVEVVDKKSKLLSTKGVAEDQKI
                     NDLLTDFHNTAIADATAPVGEMVGGNLADKNEIKEIPQSLVEDQGITDFINEVQLYNS
                     RKHLESKNINPDEVYMVSGAALFSASSNLYEGNISKADISNIYRYDNKLYTIKTNGKQ
                     LKKYMEKNAEYYNTYKDGDLTVSFNEDVRMYLYDMFDGISYEINISKEPGQRIENLKF
                     EKDGKPVEDSDVVYLTVNDYRYNSGLAAGIMDQGEHEKIYDTNNDQISDMRDLIVDYI
                     QNVKGGKITKKVNNNWKITGNDWDSEQRELAVKLVNEGKIKIPVSEDGRTPNVKAVTW
                     NDVLEALGTKEVEIPIVTFNDFHGSLAESKSDVGAAKLVGEIKRVKEENPNTVVVTGG
                     DIYQGSAMSNLLKGEPVTAMLKEMGLEFSAVGNHEFDWGYEYIPDWAKAGEFDFLASN
                     IYEKETGEPVEWAKPYGVVEREGKKVGFIGLATPETAYKTKPDNVSHLEFKDPVEATK
                     IWVDYLENEENVDAVVVLSHLGSEQNRETGEITGEIVEVAEVPGVDAIISAHSHQKVE
                     GKVNGVPVIQAYKNGRNLGYVNLKFDYKDELVVTTKLDDISKRKDTLPVDKNMKDILA
                     NFEADLAPIMNEKVADLSVDLPHDRDTGVSPMGATVTETMRRIVDADIAITNGGGVRA
                     PLMAGTVTVGDMYTILPFDNTLITMEMKGSDIIKVLEHGIEPDNFGWGQHAGVKLWYT
                     PGAERGEKITSVRLADGTKLENDKYYKVVTNDFMAVGGDSYDFSAAKNVVDTNLVIRD
                     EMANYWKENGIDPITDLATLLEAGEDTTVDLEKPENPGNPETPENPDNGENLPNTGMP
                     FGTGAVAAMGTVLTIGGLAVLRKKKNNAA"
BASE COUNT         8483 a         5410 c         2974 g        11048 t
ORIGIN      
        1 aactttttaa cgtgatatct tctacataat aatttaaatt ctaaagctca tctagatatt
       61 ctcttcatat tgtttgtaag ttttattata tttttatata gaatctttta gtttttttaa
      121 agcattccac catctacagt taatatttgt cctgttaagt atgaagaatc ctctgaagct
      181 aaaaattttg ccactcttcc aatctcttca acctctccaa atctattcat aggaatttct
      241 tcctctaggc cctctttttc ttccttacta agccatgaat tcatctctgt atttattaca
      301 cctggagcta tacaatttac tcttattccc caaggagcta tttcttttgc taaggcctta
      361 gtaaataaat tcattcctcc cttagtagtt gaatataaaa cttcgcagga agctccaaca
      421 tttccccata ttgatgatat atttataatt gatgaattac ttccttctct taataaatcc
      481 atagaatccc ttgtaagctt catagcacct attaagttaa cattcataat agaatctatc
      541 tcttcatcac tagcatccat aaataatcct atttttgaaa ttccagcatt atttactaat
      601 acatcaagtt ttccaaactt atttttaatg gaacttacta tattatttct ctcatcctta
      661 tttgctacat ttccacctaa aatttctcct gaaccaccta gttccttaat catatttaag
      721 gtttcattag ccccttctaa atcactatta tatccaacta aaacaaaagc tccagccttt
      781 ccaaattccc ttgctattcc tcttccaatt ccctttgaag ctcctgttat atatactatc
      841 tttcctaata attttttctc cattaatcct tcatcctttt ctgcataaca ttaaaattct
      901 taaaagttgt attaaaataa cttattaaaa cttattttca taaatcatta tataactaag
      961 ttataagact atcttagaac atctcttatt attctctctg cattcttata atatattttc
     1021 tctatttcct ccgctttaaa tccttcaatt tccaaagcat ttaataataa attcatctta
     1081 cttgaatctt taatttcaac ttcattttct ataccatcaa aatcagatcc taaggaaata
     1141 acatctattc ccccaatatt tcttatgtgt tttatatgtc taaccatatt tcttatggaa
     1201 gctaaattta aatcctcttc ccactcttct tttaagaaag cattgcaaaa atttattcct
     1261 gttactccac cattattagc taactcttta atcatatggt cagttaaatt tctgctatga
     1321 ttagtctttg ttctactatt agaatgagat gctataattg gactctttga atattttatt
     1381 gcatcataaa atcctccatc agaaagatga gaaacatcta ttaacattcc tagattattc
     1441 atttcttcca caacttctat acccttttta gtaagaccct tatccttgta tttaaactca
     1501 taatttggat atcctaagtc atttatataa ttccaagtta aggttattaa agaaactccc
     1561 tcttctttaa actcccttaa cttttcaata tcacccttaa ttgcatctcc ctcttcaata
     1621 gttataaaag cccctatttt accgtctttc tctgcctttt ctaaatcact tatatttctg
     1681 cataagacta tatctcttga attctcattt atttctcttt taaaattctg tagcatttct
     1741 ttgcaataag aataagtatc aaaattacat tctttattta taaacaaagc aaaaaattgt
     1801 gctaaagcct ctccttctct taatttattt atatcaacct tcaaatcatt tctaaaaagc
     1861 tttttatttt cttgaagcat acgtgatgct gtatcacaat gaaaatctat aaatttcata
     1921 aaaatcttca taggtaaatt cctatgaaag ttcacctctt tcatctagta atatgaatat
     1981 tttcacaata taaactatat caaaattttt tctcttttta aattattctc ttgtaatact
     2041 attattatta aaaaattaaa gccatgttac aaatttaaat tgcaacatga ctttaattct
     2101 ttattcagta tttcttaaag agaatactaa attcaccaaa gcaaaatttt aatactcccc
     2161 ttaaaaacta cttttaatta ttaatataaa ggatacttag cacataaagc tttaactctt
     2221 gcttttaatg gttctaaatc tccatctttt tctttaatag catcatttat tatagaagct
     2281 atttctttca tttcttcttc tttaaatcct ctagttgtta tagcaggagt tcctattcta
     2341 acaccgctag taacaaatgg gcttcttgtt tcatttggta cagtattttt atttaaagtt
     2401 attcctatac tatctaaaag aatttctgca tctttaccag ttatatcttt attagttaaa
     2461 tcaacaagta ttaaatggtt atcagttcca tttgaaacta atttaaatcc ataagattct
     2521 aaagcttctg ctaaaacatg tgcattttta acaacttgct ccatatatgt tttaaaactt
     2581 ggttctaatg cttctttaaa acaaacagcc tttgcagcta ttatatgcat taaaggacca
     2641 ccttgtattc ctgggaaaat atttttatct aatacttttg caaatttttc tttacaaagt
     2701 attaatcctc ctcttggtcc tcttaaagtt ttatgagttg ttgatgtaac aaaatcagca
     2761 taaggaactg gtgatggatg taagcctgta gctacaagcc ctgcaatgtg agctatatca
     2821 accattaagt atgctcctac ctcatctgct atctctctta aagttttaaa atctataatt
     2881 cttgaatatg cactagctcc tgcaactatt aatttaggtt tatgtttaag agctaattct
     2941 cttacagttt catagtttat tgtttctgtt tctttatcta caccatatga cacaaaatta
     3001 aataatctac ctgaaaagtt aactggtgaa ccatgagtta agtgtcctcc atggcttaaa
     3061 tccattccta atacagtatc acctgactct aatattgaaa aataaacagc catattagct
     3121 tgtgatcctg aatgtggttg tacattagca tgctcagccc caaataattt ttttactctt
     3181 tctcttgcta aatcttcaac ctcatctact acgtgacatc caccatagta tctttttgaa
     3241 gggtaaccct cagcatattt atttgttaaa tatgatccca tagcttccat aacagctttg
     3301 ctaacaaagt tttctgaagc tataagctct atactatttt cttgtctttc cttttccttt
     3361 tgaacaagat gtgcaatttg ctcatcttct ctttctaagt tgtcaaaatt cattttcttc
     3421 accccaaacg taaaatgtat attacttatt ataaatttat taattatttt tagcaacaaa
     3481 aaataatttg tttatatttt gattaaacta atatgaattt gtatagatta ttttaacttt
     3541 tcatatgtta taattactat attggttata aatttacaca ggggagaata atatggattt
     3601 tgacaaagta ttacatattg caacttttgc aggtagaata atactagaaa gcggtggaga
     3661 aacttacaga gttgaggaaa ctatttgtag aatctgtgca gcttacggtg ttagatatgc
     3721 tgaaagtttt gttaccccaa ctggaattat ggtttctgtt tacgatgaga ataattatac
     3781 aactacttta gtaaaaagag ttactaatag aactgttgat ttacagaaaa tccacttggt
     3841 taatgaactt tcaagaaaac tttatacgga aaaatattct cttgatgaag ttttagaaaa
     3901 attaatagat ataaatgaaa actgtcctaa atacaatttc agaacaaatc tagtattttc
     3961 agccataaca gctgctggat tcaccactat attaggaggt aatatagaag actgtgtagt
     4021 atcatttttt gttggaatgt ttgtaaaact agctactttt aaggctggag aattaggaat
     4081 aaacgccttt tttataaata ctctaggtgg agcaatagct gctctcttat ccttaatagc
     4141 ctatagctta ggagttggaa gcaatctaga cacaatgata ataggtagta taatgctatt
     4201 agttcctggc ttatctatta caaatgctat tagagacaca atagctggcg atttaatatc
     4261 aggacttata agggcagctg aggccttctt ggtagctatt tctatagcta tcggaactgg
     4321 tatgattatg agtatttgga tttactttgg aggtggtata taattggttt taattaaaga
     4381 agttctagct accttcatag ctaccatagc ttttggagta ttatttaata ttcacggaaa
     4441 gaatttattc tttgccggaa taggtggagc cttaggttgg ctagtttata aaataggatt
     4501 atattttgga ctaagtgtaa tattatcaat gttcttttca gcaattgcct ttagtgctta
     4561 ttctgaaata tatgcaagaa ttttaaaaac tccagtaacc acaatagttg tttgcgcttt
     4621 aatcccctta gttcctggag ggggaatgta taatactatg tacgaagcta taaagggaga
     4681 tgctatgtca acttggaatc aagccataat gacaatttca tcttcaggtg cattagcctt
     4741 aggagtttta tttgtctcta caatcactcg tttgataaac aatagaaaaa aacaatatac
     4801 tcatggatca ttaaatccta aaaacatatt attatttaaa aaattaaaaa acaaaagcaa
     4861 tatagatttt catacaaaaa aatagaccac aaaggtggtc tattatttta caaaactatt
     4921 taatgttttt attaattgtt ctaagcctgc taattcctct ttatcctctt ctcctatacc
     4981 catacaattt ctagcatagt tttctataag aagtacccca actttgttta tagcagctct
     5041 tatggcagat atttgagtta atacatccct acaacaaaca tccttctcta ccattccttg
     5101 aattcctttt acttgcccct ctatttttct taatcttaca agtatatctt ttcttaaagc
     5161 ttcattatca ttcaaaattt acacctcata ccattattgt aatctaatat ataatatttt
     5221 taatttatat tattatttca tattaattac ttaatatacc ttaattacta tttgaaagtc
     5281 aataaaaata gcaatctata attaagattt actttttaaa tggtaaagta ataaaaactc
     5341 ttaacttata aagtataagc ctttttactc attttccata taaattgact aaaattagcg
     5401 tgagtaaaaa cccacgctaa ttaagtttac tttagtatct caatttagat tgctcaaaaa
     5461 ttattctgcc gtatcttctt tctttattgt ttcaattcct aattctttta attgttcttc
     5521 atctactata ttaggtgctt cagttaaata acagaatgca ttttggtttt tagggaatgt
     5581 tataacatcc ttgatatttt cagttcctgc taagaacatt atcattctat ctaaaccgaa
     5641 agctaatccg ccgtgtggtg gtggtccaaa tttaaatgct tctaataaga atccaaatct
     5701 ttcccaagct gattcttgag taaatcctaa tacttcaaac attttttctt gaagtttagt
     5761 atcatgtatt cttatagatc ctccacctaa ctcttctcca tttaatacta agtcataagc
     5821 cttagctcta actcttcctg gatcagtatc taaatacttt atatcctcat ccattggcat
     5881 tgtaaatgga tggtgagctg caaagtatct tccttcttct tcatcgtact caagaagatc
     5941 aaactctgtt atccatgtga agttaaactc actcttatct ttaactagat caaattttct
     6001 tgaaagttct aatcttaatt ctcctaaagc ttttaaaact actgagtttt tatcagcaac
     6061 tataaggatt aaatctcctg ttttagctcc catagtttca attattttgt ttagctcttc
     6121 ttcactaaag aatttagcta ttggtgattt aacaccctct tcttttaatt gaatccatgc
     6181 taacccttta gctttgaaag ttttaacgaa ctctcctaat ctatctatgt cttttcttcc
     6241 catagaagct ccgccttcta agcataaacc tctaacagaa ccaccattag ctacagcgtc
     6301 tgtgaatact ttaaatccac attctttaac aacatcactt aagtttgtga tttccattcc
     6361 aaatcttaag tctggtttat ctgaaccgta tttttccata gcatctttaa atgtcattct
     6421 ctttattgga gtttttacat ctactcctaa aacttcttta aatacatgtt ttattaaacc
     6481 ttcatttaaa gccataacat catcttgctc aacaaatgac atttctaagt caacttgagt
     6541 aaactctggt tgtctgttag ctcttaagtc ctcatctctg aaacacttaa ctatttggaa
     6601 gtatctatca aaacctgata ccattaataa ttgtttaaat aattgtggtg attgaggtaa
     6661 tgcatagaac attcctggat agtttcttga tggaacaaga taatctctag ctccttctgg
     6721 agttgacttt gtaagtattg gcgtctccat ttctaagaaa ccattttgat ctaaataatc
     6781 tcttattgct ttagttgtct tatgtcttat tttgaagata ttttgcatat ctggtcttct
     6841 taaatctaag tatctatact ttaatctaat actttcagca gcatctaaat cttcttttat
     6901 atatattgga ggagtatctg attcagataa aatctttaat tcctcagctt ttaactcaac
     6961 catacctgtt ggcatgtttg gatttactga ttctctctta actatttcac ctgttacagc
     7021 aatacaatat tcaggtctta ttgattttgc tttttctagg atttcttctc ctaaatcatc
     7081 attaaataca acctgtaaaa ttccttctct atctcttaag tctataaatt gaagacctcc
     7141 aagatttcta tttctttgaa cccagcccat tacggttact ttttgtccaa tatggctttc
     7201 tctagcgtcg ccacacatta tgttacgctt taatccattt aaagcttcac ccattttaat
     7261 tctcctcctg tctactactt aactatattt actatttcat ttatatctaa gctgacttcg
     7321 aattgttctc catcgctcat tctcttaatt ttaattttgt tttcttctat ttcagagtca
     7381 cctataacaa aagtatattt agctcctatt ttattagcat acttcatttg agccttaaca
     7441 ctctttccta agtgatctat ttcagcctta ataccagatt ttctaagatt aaatgctaat
     7501 ttcatagctt caagcttagc cctatctccc attgatccaa tataaacttc gcatctaaca
     7561 ggctcaggaa tttctattcc ttcttcatct aatattaaaa gaagtctttc taaacctaat
     7621 ccaaatccca tagctggagt tttaggacca tctacttctt ctactagata atcatatctt
     7681 cctccaccac aaactgttaa tccatctttt ataacttcaa aaacagtttt actatagtaa
     7741 tctaatcctc ttactatttg tggatctatg ttatactcta ttcccataac atctaagtaa
     7801 gcttttaact tgctaaagtg atcactgcac tcttcgcaga tgtaatctag tattgaagga
     7861 gcttccttaa ctatttcctt acatctcttt tctttacagt ctatgattct cataggattc
     7921 ttttcaaatc ttgttttaca agtttcacaa agattatcat agttctcttt taaatattgt
     7981 tttaaagctt cattgaattt tgctctacat tttggacatc ctaaactgtt tatatttaag
     8041 cttaatcctt ttattccaaa atcctctgtt aatgctctca taactaaaga taagatttct
     8101 gcatcaatag aggcttcttg tgaaccaaaa acttctattc catattgatg gaattctctt
     8161 aatcttcctt tttgcatttt ttcatatcta aaacatggag taaaatagaa catttttgtt
     8221 ggctgagcat cagcatataa actattttca ataaatgctc taacagctgg agctgtcccc
     8281 tctggtttaa gagttatgct tcttccacct ttgtcttcaa aagtatacat ttccttttga
     8341 accacgtcag tagtttctcc aactcctctt ttgaaaagtt cagttgcctc aaacatagga
     8401 gttctaatct ctctaattcc atattcagct gatatttttc ttaacttttc ttctatataa
     8461 tgccatttat aagcgtcatt aggtaacata tcctttgtac ccttttgagc ttgtatcgcc
     8521 ataaacaact cctcctacat aatattaaat tttttattta tataaagtat caagtaattc
     8581 tatcttacct tcaaccattt cctctattct ttttacatat tcctttacat ctttaacatt
     8641 tgcaaatctt tctatacgat ttttatcttc ttctaaaaac actaccttag aaacggcatc
     8701 tgctcctagt gcaattatag tttgcttatc ttcaatcatt tgaatattgt agatacattc
     8761 cttgttatct tttgaatatc ctacattctc catgttacca accatatttt tttgtctata
     8821 catataatat ggatgcatat ttagttctct acctaaaact ttactcatct catacatctt
     8881 attaagattt ttttgctctg caatagttat agtgttatgt aaaactaaat tttcatgaag
     8941 tcttgatgcc cttttaatag acataccatg aatagttaaa ctatctggat taagctcctt
     9001 tatcatagag catgttttag aaacctctga aatatcttca tttggaagac ctattataat
     9061 atccatattt atgttatcaa agtctaagct tcttgccaaa ttgaatttat ccactacatc
     9121 ctctgttaaa tgccctctac ctattgattt caaggtttta tcgttcatac tttgaggatt
     9181 tatagatatt ctagatactt catatctctt catagttttt aatttttctt cagttataga
     9241 atcaggtctt ccacattcaa cagtgaactc ctttattcct ttattattaa cgaaactatc
     9301 atatatatgt ttcattaata cttcaaactg ttcattattt actgaggttg gagtgcctcc
     9361 accaaaataa acagtttcta tctttaagcc tttctttgat acataatcac ttatagctga
     9421 aatttctttg cttaaagctt ctaaataagg ctcaacatct ttcttgcatc cagctatagg
     9481 atttgctgca aaggaacaat aaaggcatct tgtaggacaa aaaggcatac caacatatat
     9541 acttatgttt ttttcctctt tgtttacaaa actttcttct ctttctgcaa cttctatgca
     9601 aagcttagct ttttcttccc tagccatata attatcttca aaatatttta ttatctcttc
     9661 ctcagatttt ccttctctta ttagagataa tgctatttta cttggtctta ttccaactaa
     9721 tgttccccaa ggatattcat ccttaagagt ttcagataaa aatttgaata ttcccttttt
     9781 aagttcagtt ttaaatccgt atccttcttt gaattcataa gaaaaactat tgtctccctc
     9841 tgaaatttta accatatttt cagatataaa gacatcgtag tctctctctt catcttcatt
     9901 tacaaacttt aattccttaa aagtatagaa tatattaaac atttgataaa cgtcatatct
     9961 atactttaag tcattaagat atattttaat atacataaaa atctcctgct aaaaatgggt
    10021 ttctttgtct ttcgtgttta attgttgaac ttggtccgtg tcctggatat actactatat
    10081 catcacctaa tggtaccagt ttatcattta tactcttaag aagagtagca tgattacccc
    10141 cttcaaaatc acttcttcca attgattctc taaatagagt atctcctgta aaaagacaat
    10201 tatctattaa gaagcataat cctcctggtg tatgtcctgg tgtctctaga cattttattt
    10261 cataattatc tgagaactta agagtatctc catcttttaa atatccatct gcctttggaa
    10321 gttttccaaa aacatcaaca tttctttcta tcatatcttc atctttttta tctatataaa
    10381 aaggtatttt atatttttct gcaagttttt gcacagctcc aacatgatca aaatgtccat
    10441 gagttaataa tatcattttt ggttttgcat ctaaagattc tattacattt tcaattcttg
    10501 atgcttcttc acctggatct actattatac aatccttagt ttcttcatct ataacaacat
    10561 aacaattagc ttgatacatt cctactggaa ttgtttttat taacatatca atccctccta
    10621 aaaaatgaag tttaatttat atttttaacc gaatttatag gagtctaact tattaagcta
    10681 aactcctata aaaactttca atgtttaact tttctaaaag cctatattta ttataatgct
    10741 ttatacacaa aatagtaata tttgtttaga aattcttttt gctatctaaa attaaagtta
    10801 caggcccatc attttctatg tcaactacca tatgggcacc aaagactcca gtttcaactt
    10861 ttattccctc ttctctgcac atgcttacaa actcatcaaa aagtttctta gcttcatccc
    10921 ctccaagagc attcatgaaa tttggtcttc ttccctttct acagtctcca taaagggtaa
    10981 attgcgatat aagaagtagt tctccacaaa catcctttaa agataaattc attttatctt
    11041 cttcatcttc aaaaactctt aagtttaaaa ccttatcttt catatatttt aagtcttcaa
    11101 tagtatcctc tttgccaatt ccaactaata cattaaagcc tttatttata cttccaacta
    11161 tctcattatc cactttaaca gaggatttat tcactctttg aactacaact ctcacgatta
    11221 tgcccccttt tatttattta ctctataaac atctaatatt ccttgtagtc ttcttatttt
    11281 cttcattaat tcttttaatt gttctatatt atcaatcttt acttgaatat ttatatttgc
    11341 tactccattt ctacctgatt tagcatttaa agaaagtagt ctaaaattag agtcagttat
    11401 aactaacata acatcagata ataaacacat tctatcttct gctttaacct caagttcagc
    11461 aacatatgca gctccctttt cagttcccca actaacatct acaactttct cttcttgttt
    11521 ttctactata gctttaaaat tagaacagtc ttttctatgt acagaaactc ctcttccctt
    11581 agttatatac ccagctatat catctccagg tacaggatta caacaccttg caaatctaac
    11641 cattatatta tttaatccct taacagttac tccatagttt tgttttttct ttatttgtct
    11701 ttcagtttta gctatttgtt cttcaatatt cttatttaat tcctcttcag caacctgttt
    11761 tacaatattc tctttaagct tagatattac agtagatgaa agtaactctc cttctcctat
    11821 agctacatat atttcttcaa cattatttaa atgatatctc tttaataatt tatcatataa
    11881 tggccctttg caaagatctg aaaatactaa ggattgcttt ttacattcct tatcaagcat
    11941 ttcctttcct ctttctaaat tctcttctct tcttgctttt ctaagccatt gctttatttt
    12001 acttctagct tgattgctat tagctatatt taaccagtct atattaggtc ctctagatga
    12061 tgaggatgtt aatatctcta ctatttcccc agttttaagc ttgtaatcta gagttactat
    12121 ctttccgttt actttagctc ctacgcattt atttcctata tctgtatgga tcttatatgc
    12181 aaagtctaag ggagttgctc ccactggcaa atttataact actcccttag gagtaaatac
    12241 aaatatttca tctgagaata agtcaagttt aaacccttcc ataaactcag ttgcatcaac
    12301 agcttccttt tgccactcaa gtatatctct aagccatgtt aacttatttt caaaagtcat
    12361 atcttttgaa tcagtgccat taataccact cttatatttc caatgagctg ctattccgta
    12421 ctcagccgtt ctatgcattt caaaagttct tatttgaatt tcaaaagtct ttccttcgct
    12481 tcctattact gttgtatgta aagattgata catattaggt tttggcattg ctatataatc
    12541 tttaaatcta cctggtattg gcttataaat agtgtgtact atacctagta ctgcatagca
    12601 atctttaacc gtattaacta aaatccttat agctgttaaa tcaaagattt gttctatgct
    12661 cttatgttta ttcaccattt ttctatatat actgtagaaa tgttttggtc ttccttctat
    12721 atcgctatct atgttagctt tatctaaatt ttcttttagc tctttaatta tacgagaaat
    12781 aaatttttct ctctccaccc ttttttcagc tatcatattt actaagtcat aatattcttc
    12841 tggatgaata tatcttaaac acaaatcttc taactcccac tttattttag atatacccag
    12901 tctatgagct aagggtgcaa atatatctaa agtttcttga gcttttttct tttgtttttc
    12961 aggcttcata tatttaagtg ttctcatatt atgaagtcta tctgctagtt ttattattat
    13021 aacccttatg tctttagcca ttgctaaaag catttttctt acattgtcag cttgttgttc
    13081 ttctttgctt ttatatttta tttttccaag cttagtaact ccatcgacta aattagctac
    13141 ttcaacatta aaaatattac ttatatcttc ataagtataa tccgtatcct ctattacatc
    13201 atgaagaagt cctgcaacta tagtatttgt atccattccc atatcagcta atatcatagc
    13261 tacactaata ggatgagtta tgtatggttc tcctgattct cttttttgtt ctttatgtgc
    13321 ttcaaaagct aaatcatatg cttttttaac taaatctata tcaacattat tcccattagc
    13381 ttttatttta gaaattagtt cttctaacat actggattat tctcccctac ttaaaatttc
    13441 ttttaatata ttatattatt atataacaaa aaaataaaat aaaccaataa taaaattaat
    13501 ggctggtcaa taaaccagcc atttttaact ctattataat tttttacaaa attaaagaat
    13561 tttaaacatc ataatcaact aatgatgtta cttcgtatcc ttttaattta tctcttcctt
    13621 ttaattctgt taactcaata gtaaatgcta atccagcaac ttctccacca gcttgttcta
    13681 ctaatttagc aacagcagct attgtaccac cagtagctaa aagatcgtca actatagcaa
    13741 ctctcatacc tggttttata gcatctttgt gtatttctaa agcatctttt ccatactcta
    13801 aatcatattc tactctaact gtgtcccctg gaagtttacc tggctttctt actggaatga
    13861 atccaacacc taaagcatag gctacaggaa ctccaaatat aaatcctcta gcttctggtc
    13921 caactacaac gtcaatattc ttatctttga agaattctgc catttggtca actgaatctc
    13981 ttaagccttc tccatctgct attagagtag ttatatcttt aaaacttatt cccttcttag
    14041 gaaaatcttc tataactctt attttatcct ttaaactcat tactttccct ccaaaatcaa
    14101 taaatatata taaattttta attactacct tattttttca ttataaatat atttagttat
    14161 ttgctccgct ctatccttat catttgtagt taataactcc acaactatct tcgcgttatc
    14221 ttttttacta atggcacctt ctcttggagt tatctcatta actattttat taatactatc
    14281 tatatttata tcactaatat taaagaaact cttaatagca ttcaatccta tactattagt
    14341 atatcttaaa tattgaatgc cttcctttaa tattattcca ttttcccctt tatttttaac
    14401 gttctttgaa acactaccta taagtattaa atctaaatac ttatttattc ttttcatgtt
    14461 ataatatata gcaatagctt gcattaactt aaaagttaac cccgaattag tcaaatcctt
    14521 atatctatat gaacactttt cttcacttgg gtttatatat gttgaatcat atatgcattt
    14581 tgtgcgttta ttttccatta ttattagatc tatattaaat ctattacaga gattctccac
    14641 ctctttattt ttaaggccac aacctactgt tattaaaagt tctgcaccta aaaaagcaat
    14701 atcttctctt aaagtatatt catctatatc ataatttttc tcttcatgtt ctgaacttac
    14761 acaatattca acatcagcat ttaaatactt tagtataagc attaaagatg atattgcaca
    14821 tatggaatct aaatcacatc caccatatat aactatcttt tctctattgt taattgcaga
    14881 tgctagtctt tctattgctt tattcatatt tttaatgtta aaagggctat accctgatat
    14941 gtaattagtg caatatatgt ttttccaagc cttctgcata atttaactcc tccataaaat
    15001 taagaaaaca agtatattat aatttaaaat tacctttttg acaaatactt ttcaaataaa
    15061 tacgtattca ttagtcattt ttattacttt tctcctattt ttcttaaaaa gtaataaaaa
    15121 ttctaattaa aataaaaaaa atgctaaaag ttataaaact tttagcattt ttatactatt
    15181 acttatttat tgccttttta tttttttgtc tgttagctaa aatacaccaa attggacttg
    15241 ctataaatat tgaagaacaa gctcctgatg ctataccaac tattaatggg aaagcaaaat
    15301 ctctaactgt aggaacaaat atataaacac aaactatagt gattaaagtt gtaagtgttg
    15361 tatttataga tcttgacatt gtttgagtaa tactcatgtc tgcaatctct tccacagatt
    15421 tccctctaag tttttttcta ttttctctta ttctatcaaa tataactatt gtatcattta
    15481 tagaataacc tactattgta agtatagccg ctataaatgg agtatttatt gttattccaa
    15541 atatagcgta aacacttata gttattaata catcgtgtaa tagagctatt atagctgcaa
    15601 taccaaaaga aaattcaaat cttataatta cgtatataag catacctatt actgctatcg
    15661 caagagctac tatggcattt gttgttaaat ctcttccaac tgaagcccca atttcatttt
    15721 gtgatactaa ggcatcatct tttaaatcgt atttttcttt aagagcctta aagatatcat
    15781 taacttgaga actatttaac tcttttgctt ttatttgaag ttcagtacca ttaacttgat
    15841 ttgtaacggc atcctttgaa tattcttcta caattttatc aacatcagtt ttattaaaat
    15901 ctgtacccat gtttattaca acctgagtac cccctctaaa gtcgattcca aagttcatac
    15961 ctttagtaaa cataaatcca aaacctataa caattataag aattgatata gtaaaccata
    16021 ttttcttctt ctctacaatt ttaagcatta tgtctacctc cttttatacg accaaacatt
    16081 gaaggcttat ttaatattcc catgttgaat cctaaattca taaagaatct tgtcattatt
    16141 atagcggtaa acatacttat tacaatacct attaataatg ttaaagcaaa tcctttaact
    16201 ggtcctgatc caaagaagta tagaactaaa ccagcaataa gggttgttat attagaatcc
    16261 attattgatc ttaaagcatt gctaaaacct attttcatag ctgatgttat acttctacca
    16321 tttccaagtt cttctttgat tctttcaaat ataagtacgt tagcatctac tgccatacct
    16381 actgttaata agaaagctgc tatacctggt aaagtaagag ttactcctac taatgcaaat
    16441 atataaagca ctaataatat ataaacactt agtgacatac atgcaatgaa tcctggaact
    16501 ctatagtata gtatcataaa taagaatatt atagctacac ctattgctcc tgctttcaga
    16561 gcatttggaa gagcatttgc acctaactga gcacctacag tctcaactga aactgcctta
    16621 actggaactg gtaatgcacc agcatttatt attccagata ccttttcagc ttcttccatt
    16681 gatctatttc ctgaaataat agcttcacca ttagaaatta tgtcatttac tactggatct
    16741 gttaatgttt cattatccat ttttatagct attttttggc ctttatattt ttttgttgcc
    16801 tcagcaaact tttgcttacc ttcttcgtta agctctaatt ttataactgg cttacctgtt
    16861 tgagggtcta tatatacact tgacttttca acatctttac ctgttaaaat aacatcatcc
    16921 tcaggcccta caaatgttaa ctcccctgtt ttttgaagag aatccactat agtttttgaa
    16981 tcgtattgtc ctggaatatc aattcttatt ctattatttc cctctgtcgt tactacagtt
    17041 tctgaaactc caaccttatt aacccttaaa gatagaagtt cctttgttgt gtttaattga
    17101 tctactgtta cgtcattact ttggatttcc ataagaacag aaactccacc ttgtaaatcc
    17161 aatcctttag ttataacctc attaaacgtt ttaaattgat agtcccctaa tgtaaatcct
    17221 ttgaaccctg ctagagctaa aaaacttatc actgctatgg agataaaaaa taatatgaag
    17281 ctacctaatt ttgatttcat ggttatcccc ctctgtaaaa ttttctctga taaactaaat
    17341 tatattaata tgttgccata tagtcaatat atggcaacaa ttattaatat tatattttta
    17401 ttcagcgtcc atagtctttg actttaatgc aatagcacac tcaattcttt tcttttgcat
    17461 ttcacatcca acttcataag gaacatgaat atcgccatta aagttaaagt tattagcttg
    17521 acatccacca ctacagtaga atctagccca acattcttta cattttggtt tattatatat
    17581 gtgagcttgt ttaaattctg ttgaaagatc ttttctgatt tctccatcat agatatttcc
    17641 tagtaagaaa tcagtatttc caacaaattg gtgacatgga tatatttctc catctggagt
    17701 tacagcaata tactcatgac ctgctccaca tcctgctatt ctcttataaa cacaaggacc
    17761 accatttaaa tcaatgttaa agtgatagaa tttgaatatg ttatctccct cttctagtct
    17821 tctcaccatt tcattgtata atttatcata ctctccaaag atagttggta aatcttctct
    17881 tcttaaagat aattcatgtt catctggaag aacaactggc tctattgaaa tctcagtaaa
    17941 tccttcatta gcaagagcca ttacatcttg gaagaagtca gtattatttc ttgtaaatgt
    18001 tcctctagca taatattgtt tacttggatc tctcttttca accattttct ttatgtttgg
    18061 aagtattcta tcataacttc ctgaaccatc aactcttatt ctaacagcat cgttaacttc
    18121 ttttcttcca tctattgaaa gaatgatatt tccgatgttt ttatctatat aatcgattct
    18181 ttcatcattt aaaagagttg cattagtagt catagtaaat cttacatttt tgcctacttc
    18241 ttgtcctctt tttttaccat aatctattat ttcttttata gtatcaaata ccattagtgg
    18301 ttccccacca aataaatcaa cttctatatt ctttattcca cctgaatttg ctaatacaaa
    18361 atcaatagct tttttaccaa cttcagcact cattggtttt ctacatcctt tgtattctcc
    18421 ctcgtcagcg aaacaatatt tacatcttaa attacagtca tgtacaacat ttaagcatag
    18481 agcttttata tatgaaggtg ccttgtcact ttctttagca acctcttcat ataaatctcc
    18541 tgagtataaa gcgtcttctt ctactaattg aagtaaatct tcataagcct cttcaatttc
    18601 ctcaactgga tatttatctt ttaacttttc aattaaagat tccttactac ttaatttgtt
    18661 atcattatct atcatattgt aaataaggtc atcgacaata tgaacacttc ctgagtttac
    18721 gtctactacg taatactctc caccttgcat aaatttatga attaatgcca caatatttcc
    18781 tcctaactaa gaactatggc agcaacttta aaagttactg ccacttaaga atttttataa
    18841 ttaattttca caagctaagt ttgctactgt agctgatgtt ttacaagctg attggcatga
    18901 gttagcacac tctctgcaat ctgctggttt acataagctc ttttttaagc ttgacttgtt
    18961 tactgttttt atacgcttca tttactcttc ctccaatcaa aactatcttt aatattatac
    19021 catgttaagg tttaatttta aagttctttt taaaaagcta atttgattta cccccttatt
    19081 ctttcacatt ataatataaa attcaatacc taaaatgaaa aaatagttat tttaatgcat
    19141 aaaatacatt aaaataacta tttctttaaa ttatactaat caatctttga acttatgcct
    19201 tgttttgaaa actcgatctt tgttctatct tgagtagtct ctattataac gctatcatct
    19261 tttaatttta ctattctacc tatgattccg cctctagtta tgatcttatc attaactttt
    19321 agctcatcta tcatagcatc atattttttc tttcttttct tttctggtaa gataagtaag
    19381 aaataaaata caccaaacat taaaataaaa ggtaatacca ttgttgctat ttgctgaaaa
    19441 ttcatactag ccactccctt cttattatgt aaattttata ttattaagct cttccattcc
    19501 actcttctaa ctttttagct ttgaattctg caaagtatcc gccatctata gcatctctta
    19561 tatcctccat aagcttatta tagaagtata gattgtgaag aacacataat ctcatagcta
    19621 acatttcttt agccttaaat aagtgtctta tatatgctct tgtgtaattt ttacatgcag
    19681 gacattgaca tccttcatct attggtctag catctaattc aaacttagca ttcattaagt
    19741 ttattttacc ttctttagtg aaaacatgac catgtcttcc atttctagca ggtaaaacac
    19801 aatcaaagaa gtctactcct ctttctactg cctctaatat atttgatgga agaccaaccc
    19861 ccattaaata tattggttta tcctctggta agtgaggaac tacagcatct ataactctat
    19921 acatttcttc atgagtttct ccaactgcta atcctccaat agcatatcca tctaaatcca
    19981 tttctcttat agttttagca tgttctattc ttatatcttc ataaactcca ccttggttaa
    20041 taccaaaaag catttgctct ttattaacag tgtcatctaa tgaatttaat ctatccattt
    20101 cctttttaca tctttcaagc catcttgttg ttcttgcaac tgacttttct acatattctc
    20161 tagttgatgg gtttggaata cattcatcaa aagccatagc tattgttgaa cctaagttac
    20221 tttgtatttg catactttct tctggtccca tgaatatttt tctaccatct atgtgtgagt
    20281 taaaataaac tccctcttct tttatctttc tcattcctgc taatgagaaa acttggaatc
    20341 cacctgaatc tgttaagatt ggtctatccc aattcataaa gttatgtaaa cctcccattt
    20401 gctttacaat cttatctcct ggtcttaaat gtaaatggta tgtattagaa agttctactt
    20461 gacatcctat ttcttttaaa tccattgaag aaacagcacc ttttatggct gctaaagttc
    20521 ctacattcat aaaaacagga gtttgaattg taccgtgagg agttacaaac tcacctcttc
    20581 tagcttttcc gtcttttttt aaaagagtat atcttttttt agtcatgaaa aaagtccttc
    20641 ctttgctttt tattttataa acattgaatc tccaaatgag aagaatctgt atttttcttt
    20701 tactgcggtg ttataagcat tcattatatt atcttgacca gctaaagctg aaacaagcat
    20761 tattaaagtt gattcaggta aatggaagtt tgtaattaaa ttatcaacta ttttaaactt
    20821 atatcctgga tatatgaata tatctgtcca tccactttgt tctctaactc ttccattttc
    20881 atctccaata gtttctaaag ttcttgaaga tgttgttcct acagcaatta ctctctttcc
    20941 agcttcctta gttttattta taagttctgc attttcttta tctaaatgat aatattctga
    21001 atgcataaca tgattattta catcatcaac ctttactggt ctaaatgtac caagtcctac
    21061 atgtaaagtt aaatatgcta tattaacacc tttatcttta atttctttta ataaatcctc
    21121 agtaaaatgt aatccagctg ttggtgctgc tgctgatccc ttttctttag aataaactgt
    21181 ttgatatctt tctttgtctt ctaacttttc atgtatataa ggtggaagtg gcatttgtcc
    21241 taattcatct aaaacttgtt caaatatacc atcatactta aactctatta ttctgtttcc
    21301 ttcttcacca atttctctaa ctatggcttt aagtttacct tctccaaaag tgaaaactgt
    21361 acctacttta gcttttctac ctggcttagc taaacactcc cacttatctc cttctattct
    21421 ctttaaaaga agaaattcta ttttaccacc agtttcttct ttttctccta taagtctagc
    21481 aggtaatact ctagtattat ttaaaactaa agtatctcct tcatttaaat actctaatat
    21541 atcatggaat tttctatgtt caatctctcc agttttttta tctaaaacca ttaatcttga
    21601 agaatctctc ttttcaagtg gatattgagc tataagttcc tctggtagct caaaataaaa
    21661 atcacttact ttcattttaa ttacaccttc tctattttat ttttcaaata acgtacactg
    21721 tttagaatct atcttagatt cattaaatct aactcttcct aagtgtttat acgctttatc
    21781 tgttgccatt cttccccttg gagttctaac tataaaccct ttttgtagaa gatatggctc
    21841 ataaacatct tctatagtat ctaattcctc tccaacgaag taagctaagg tttctatccc
    21901 tactggtcct ccattgaagt tatctattat ggcttctaga attttattat caattctatc
    21961 aaaaccttct ccatcaactt ctaaaatctc taaggatttt tttgcagcct cttctgtaat
    22021 ttcattatca tataaaactt ctgcaaaatc tctcactctc tttaaaagtc tatttgctat
    22081 tctaggagta ccccttgacc ttttagcaat ttcaaaggcc ccttcttcag ttatatggca
    22141 tcctaaaatt tcagcacttc taattattat ttcctttaat tgttcatcag tataatattc
    22201 catagaacat aaaaccccaa atctatctct taaaggtgaa ctaagcatac caattctcgt
    22261 tgtggcacct attagagtaa atttaggtaa atcgagtctt atggatttac tagcagcccc
    22321 cttaccaata attatatcta aaacataatc ctccatggca ggatatagta tttcctctac
    22381 acttctattt aatctatgta tctcatcaat aaataaaaca tcattagtgt ttaaggttgt
    22441 taatatcgcc gctaagtctc ctgctctttc aatagcaggc cctgatgtta ttttaagatt
    22501 tccacccatt tcatttgcta ttatattagc taatgttgtc tttccaagac ctggtggtcc
    22561 atataaaatt acatggtcta aggcttcccc tcttctttga gcagctttta tgaatatgtt
    22621 taatctttct ttaactttgt cttgacctat atattcattt attttctctg gtctcaaact
    22681 gtattcatta gttgaatcta ttccaatctc attagaggta actaatcttt cactcattta
    22741 attacacctc ctaacgcatt aaaagtttta aagactcctt tatcatattt tctatagata
    22801 atgtcttatc aactttttct aaagcttttt gagcttcctt ctctgtatag cctaatgcca
    22861 ttaaggcact taatgtttca tttatattga aactataatc agaattatca ttaattcctt
    22921 ctattaattg ttcctcttca gaagtaagtt catctggttt taacttatcc ttaagctcta
    22981 gaataattct ttgagccgtt ttctttccta ttccaggtgc tcttgttata tgcttttcat
    23041 ctcccatcat tattgcgtac tttaaattat taacagtact aatggataaa agtgataaag
    23101 cagcttttgc tcctactcca tttatggata ataaaagctt aaacatttct aattcttctt
    23161 ttgttgtaaa tccatataga cctatgaaat cttctctaac gatctgctct aaatatagaa
    23221 gaacttcatc tcccactttt ggcatattag acattgtatt tccagatgtg aaaattctat
    23281 atcctatatt atttaattct attacaacat aatccttact tatcccttga aattggcctc
    23341 ttatatattc gtacatattt aatgctccta tgtataataa gttctttaaa tctatataat
    23401 actttaacat gaagagggaa tattatcaat gaattacaca aaatttacta ttcattaaaa
    23461 acactctaaa ctctctattt tcctagatca tactttcatt taagtaataa aaaaagccac
    23521 atcttactaa gacatagcta ttgattatat atactttttc aaatgataaa tctacttaca
    23581 tttttaactt atataatttg ttatattttc ttcacactct tctcttgtat catatttctt
    23641 ttcaaaatta cttattttac ttctatcaac ctctggcaaa tcttctatct tctttgtgct
    23701 aacatcctct ggattttcaa taaatggtct tccatcttca ccagccttaa atattgctat
    23761 gtacccatct ttttcaccta tgtaatattt accagcttct aatattccca tatcatttaa
    23821 aaaaattatt tctcctgaag ttgatgaaac taatttatac ccatcttttt ctacagcaga
    23881 aattaactcc tccatatttt ctccattgat ttcatcattt ttaaccattt ctccaatagt
    23941 agttttataa tcaactacaa catggtcatc taaagttctt cttgtaaata ttacatccat
    24001 attctcagct aaaacaagtt cacctttttt ctctgtaacc ttcatattag tttgttgaat
    24061 tttcctacta ttataaaagt aagttatcat ataactgctt gcaaatataa atatgcttaa
    24121 aagaataaat attaagtttt tgattttatc attatttttt atattattaa aattcatatc
    24181 atcacctcat tggtgattat taacatattt tctcttttta tacacacatt ttatgttttt
    24241 tatatcaaaa atcaaaatta gactaaagta tatcataaaa taaaaaggga gtgattaaaa
    24301 ttcactccca aaataattaa ttattatgca gcattattct ttttctttct taatacagct
    24361 agtccaccaa ttgttaatac tgtacccata gcagccactg ctccggttcc aaatggcata
    24421 cctgtgttag gtaagttttc accattatca ggattttctg gtgtttctgg gttacctgga
    24481 ttttcaggtt tttctaaatc gacagttgta tcttcaccag cttctaataa agtagctaaa
    24541 tcagttatag gatctattcc attttctttc caataatttg ccatttcatc tcttataact
    24601 aaatttgtat ccactacatt tttagcagct gagaaatcat aagaatctcc acctacagcc
    24661 atgaaatcat tagtaactac tttataatac ttgtcatttt ctaatttagt tccatcagct
    24721 aatctaacag aagttatctt ttctcctctt tcagcccctg gagtgtacca aagtttaaca
    24781 ccagcatgtt gtccccatcc aaagttatct ggttctattc catgctctaa aacttttatt
    24841 atatctgaac ctttcatttc cattgtaatt aaagtattat caaatggaag tattgtgtac
    24901 atatctccaa cagtaactgt tccagccatt aatggggctc ttactcctcc accattagtt
    24961 attgctatat cagcatcaac tattcttctc atagtttcag ttacagtagc ccccatagga
    25021 cttactccag tatctctatc atgtggtaaa tctactgata aatcagcaac tttttcattc
    25081 attataggag ctaaatcagc ttcaaaattt gctaatatat ctttcatatt cttatcaact
    25141 ggaagagtat cttttctctt agaaatatca tcaagtttag ttgtaactac taattcatct
    25201 ttatagtcaa attttaagtt aacatatcct aaatttctac catttttata cgcttgtata
    25261 actggtactc cattaacttt tccttcaact ttttggtgac tatgagctga tattatagca
    25321 tcaactcctg gtacttccgc aacttcaaca atctcaccag ttatttctcc agtctctcta
    25381 ttttgttcac ttcctaaatg acttaaaaca acaacggcat ctacattttc ttcattttct
    25441 aaataatcta cccaaatctt agttgcttct acaggatctt taaattctaa atgagaaacg
    25501 ttatctggct ttgtcttgta tgcagtttca ggagtagcaa gtcctataaa tccaactttc
    25561 tttccttctc tttcaacaac gccataaggc ttagcccatt caactggttc tccagtttct
    25621 ttttcatata tatttgatgc taagaaatca aattctccag ctttagccca gtctggtata
    25681 tattcatatc cccagtcaaa ctcatgatta ccaactgctg aaaattctaa gcccatttcc
    25741 ttaagcatag cagtaactgg ctcacctttt aaaagatttg acatagcact tccttgatat
    25801 atatctccac ctgtaacaac tacagtattt ggattttctt cttttactct ctttatttct
    25861 ccaactaact tagctgctcc aacatcactc ttagattctg ctaaagatcc atggaaatcg
    25921 ttgaaagtaa ctattggtat ttcaacttcc ttagttccta aagcttctaa aacatcattc
    25981 caagttacag cttttacatt tggagttctt ccatcttctg atacaggtat ttttatttta
    26041 ccttcattta caagtttaac agctaattct ctttgttctg aatcccaatc attacctgtt
    26101 atcttccagt tattattaac ttttttagtg atttttccac cttttacatt ttgaatataa
    26161 tcaactatta aatctctcat atctgatatt tgatcattat ttgtatcata tattttctca
    26221 tgctcacctt ggtccattat tccagcagct aatccagaat tatatctata atcatttact
    26281 gttaagtaaa ctacatctga gtcttcaact ggcttaccat ctttttcaaa ctttaaattt
    26341 tcaattcttt gacctggctc tttagatata tttatttcat aacttattcc atcaaacatg
    26401 tcatataaat acattctaac atcttcatta aaagatactg ttaaatcccc atctttataa
    26461 gtattataat actcagcatt tttttccata tactttttaa gttgttttcc atttgttttt
    26521 atagtatata atttattatc atatctataa atatttgaaa tatctgcctt gcttatattt
    26581 ccttcatata agtttgatga agcactaaat aaagctgctc cagaaaccat ataaacctca
    26641 tctggattta tattctttga ttctaaatgc tttctactat tatataattg aacttcattt
    26701 ataaagtcag ttataccttg atcttcaact aatgattgtg gaatttcttt tatttcattt
    26761 ttatcagcaa gatttcctcc aaccatttcg ccaacaggtg ctgttgcatc agcaattgct
    26821 gtgttatgaa aatcagtaag taaatcattt attttttgat cctctgctac accctttgtg
    26881 cttaataatt tagacttttt atctacaact tctacttttt ctccattttt tctaacagtt
    26941 aattctattt tagaaaccat ttgaccatta cttgtaggtt gacttataac tgcattgttt
    27001 attttttctt ctggtacatt agtatgacta tgtcctgcaa ctatggcaga tacctctggg
    27061 ttcatttcag ctatggctct agcactatct ccattgccat attcactttc taatcccaca
    27121 tgtgaaacta ctgcataaac atcagcacca ccctcaggtc tattttctat ttcatctatt
    27181 acaaatccaa cttcttctgc aggattttta gcctcatatc cttttagatt ttctgaatcc
    27241 catttcatta tatgaggact tacaactcca attaaagcaa cttcaactcc ttctatttct
    27301 ctagttgtat atccttcata aaccctctta ccatctttat aaagatttgc acataataca
    27361 tctaaattat cattagcttg acttacaaca ttattaagat tgtccattcc atagttaaat
    27421 tcatggtttc ctaaagagaa aacatcatat cctatagcat tcattcccat aaccatagga
    27481 ttaggaccat ttttaaataa gtgattaaaa ttcccttgta tagtatctcc attatcaacc
    27541 actattgtat taggattttc ctttctagct tcattaacta atgtggatat ttgatttaat
    27601 ccaccatcag atttttgatt acgagcatac tcataattca taaatctacc atgaaggtct
    27661 gatgtaccta aaatctgaat agttacatct ccttcttttt ttaaactagc ttcttctcct
    27721 tttttctcta ggttttccgg tgctaaattt tcagtagcct ttacagtagt taacggtgtt
    27781 gtaattccaa agatcattga agtagcaact gctaatgctg taaatttctt atcccttact
    27841 ttcttaagca tattttcccc ctcatttagt ttactatttc tataaaagta aattttatac
    27901 atatttattg tacat
//
LOCUS       DAEVGI010000017        27899 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEVGI010000017 DAEVGI010000000
VERSION     DAEVGI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 27899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27899
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.017"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(122..6436)
                     /locus_tag="K8M77_000699"
     CDS             complement(122..6436)
                     /locus_tag="K8M77_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HBI7085215.1"
                     /translation="MFSKKFKKQMAYVLTSTFIFGLVNPSAIKAMASTDVEIAEGVTV
                     TAKGNTEGNTADLTIDGDLSTYWESSNDYKWIEVDLGGIYELSKIEIFNKDEAVYKYN
                     IYASEDGENFNKIAYKNNDNVSDSNGNVHTIDNVRAGKIRIDVVQNSNSDRVNIAEIN
                     VFGKNTGESLPEVKKIATSNFSETPWATEYEKFNSDSAYANEKTLNEIKNLVGRVIGE
                     KFKDKFIFEIRDQLNGNDVFEVSNSRGGKVLIKGNNGVSLASGFNYYLKNYCNVSYNP
                     IMESNLKMPETMPSVGERVVIDTPYEHRYALNFCTYSYTMAFWDWDQYEEFLDWCAMN
                     GVNLVLDIIGQEEVLRRTLNEFGYSDEEVKEFISGPAYFAWFYMQNMTGFGGPLPNDW
                     FEQRAELGRKMHDRMQSFGINPVLQGYSGMVPRDFKEKNPEAQTISQGGWCGFDRPDM
                     LKTYVNEGEVDYFQNVADVFYEKQKEVFGDVTNFYGVDPFHEGGNTGDLDNGKIYEII
                     QNKMIEHDNDAVWVIQNWQGNPSNNKLEGLTKKDQAMVLDLFSEVSPDWNRLEERDLP
                     WIWNMLHNFGGRMGMDAAPEKLATEIPKALANSEHMVGIGITPEAINTNPLAYELLFD
                     MAWTRDQINFRTWTEDYIERRYGKTNEEILEAWNIILDTAYKKRNDYYQGAAESIINA
                     RPGFGIKSASTWGHSKIVYDKSEFEKAIEIFSKNYDEFKDSDAFLYDFADILKQLLAN
                     SAQEYYEVMCNAYNNRDAEKFKFVSGKFLELIKLQERVLSTRPEFLIGNWIEDARTML
                     KDADDWTKDLFEFNARALVTTWGSRNNADGGGLKDYSNRQWSGLTGDYYYARWEKWIN
                     GLQIELDGGAKAPNIDWFKMEYDWVNKKSDTDKLYPTEASNENLGELAKIAMESYSVT
                     NMDKILGENESVERVNIALGKPVISETHGETEANPLSNITDGNTGTMWVADREGYPAS
                     VTVDLQGVKSVDGIEFAFRNQAGDRAVAYKVEVMDESGQWKTVSDKSEDYSSKGVIEK
                     VDFTGKAQKVRVSLTNVDLNLSPSYKIPELAEIMVYGGKEKEDEYTNVALGKPVNGAE
                     AENGKPLSNITDGDLNSLWVSNNGQTPATTSIDLQGTENVEFLELHFEKLGLRFQFKV
                     EVEDANGNKETVLDMTSNKEDNEKSYKIPVGKDINKVHVTMTGKAQGGQHPGAWAAIA
                     EVKAMSKEKPANYVNIAENKNVSGSNSQSGNPLYNITDGDLSSLWISDNGAIPANATI
                     DLEGNSFVDFLELHFEKEGFRFKFKVEVEDESGNRETVLDMTSNTEDNKKSYNIPVKK
                     EISKIHATITGKADGGEYPLAWAAIAEIKAMSKEAINKVEQASIVLPQGNMKATATSE
                     HPDVGNEGLAKFAIDGKENTIWHTKYSPVDNLPQSITLELGGSYEINKFTYLPRSGAK
                     NGNITKYELHVSEDGNDFRKISEGNWDDGGSLKTLKFNSTKATHVKLVALEGVGGFAS
                     AAELNVFAIPENLAMKDETKVEVTSNNSEANNLRDGNENTLWVPGQEEEKSVTFDLSK
                     EKDISAIDIVSKGNSPLKYSIEISNDGTEWTKIVDENNNEENKAVYSNILKLGEMGRF
                     VRFNFNSENVKIGEIKIYKGDSTSSLASYIRVVEDIYNSAVVGDMAGNYTEEAKESLG
                     NAIDEAKLALGNDINSKEANEVMSTLKSALNEFYKGFVQINRTKLAALISDVDILLNE
                     ILPSLMNSMNYNEKAIVNAEIQKLKLENNEAKEVYNKKQVSQSEIDTAYDELKNTLDS
                     YYDAVSAETAYKSRILLAKKKVENAVVGNGNGQYPKEAVDALNEAIRIAEEGFESSTT
                     LEEIINVTKTLKEAIDTFVESVNVIDKSQLKEIISSIESKLENAEGVYIPNAIEELKK
                     ALDKANEVLNNEGANQEDINKAISDLTEAEAKFELSKVPNKEELNFEIERAYDVLEKL
                     KGVDHIEGIKIKLEDAILEAERVKNDENSLKVDVDKVLGELKIVIEEALKAIDSSEVI
                     VSPVRDFKATNISKKNVTVQWSAPENSFGLEGYVLYKDGKKVAELGKDETTYKFKGLN
                     RHTIYNFKIAAKYNNGELSSKEAITLRTER"
     gene            complement(6777..6950)
                     /locus_tag="K8M77_000700"
     CDS             complement(6777..6950)
                     /locus_tag="K8M77_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBI7085216.1"
                     /translation="MSHKNNKNSKDNKKTSQKTKKEVEKMKNETANEIGYSKESRKLG
                     KDKTRNNPYMGPQ"
     gene            complement(7057..8433)
                     /locus_tag="K8M77_000701"
     CDS             complement(7057..8433)
                     /locus_tag="K8M77_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7085217.1"
                     /translation="MIITRDKRFYRLLFSIALPIAVQNLITFMVSMVDTLMVGALGEI
                     QLSAVSIANNLFFVLTILMFGLAGGSNIMISQYWGKGNVKTIHKILAIMYRVCLLITG
                     IFIFIALFLPKYFMGIFTTDKAVIDFGASYLRIVCIGYLFYSITNCTIMMLRSVKTVS
                     ISIIVYTASLVVNSILNWIFIFGNLGAPELGIRGAAIATICARITEFSIVLVFMFIYE
                     RKIGLKIEHLLKLDKEILKDYVGLCTPVLCNELLWAIGASMISVIVGRMGTEVVAANS
                     INGVAHQFVTVFIFGMSNATAVIIGNTIGEGKKEKAKEYAYSIGIFSVVMGCISGLMI
                     LLIKPFVVNFYNVSYSTKLIAMEIMTVTSGIIVFQSLASNFMMGVLRGGGDAKFVLIN
                     DLIFMWLVAIPGGFFVAFVLELPVVLVFLVIKCDEILKSLTSVYRVISGKWVNDVTKD
                     YEFEEVKC"
     gene            complement(8703..10139)
                     /locus_tag="K8M77_000702"
     CDS             complement(8703..10139)
                     /locus_tag="K8M77_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7085218.1"
                     /translation="MKFNELNLSEDIIKALNNLGYKNPSEVQEASIPKLLKGEDIIVK
                     SQTGSGKTASFGIPVCEKINIEEKKVQCLVLTPTRELAIQVKDELSNIGRIKKVRCAA
                     IFGKQPFNEQIRELKQRVHIVVGTPGRVFDHMERGTLDLSEVNYVVIDEADKMLSMGF
                     IDQIKDILGELRLNKTTALFSATFSKEIEEICEEYMRNPMLLEVKSKVFNRDRIEESA
                     IEIEEKNKGKALWKCLYAYHPESAIVFCNTRNKVKSVYKMLKDEGITCNELHGDMEQK
                     KRIEVMENFRDKHFKVLVCTDIAARGIHVDSIELVINYEVPMETESYVHRIGRTGRAG
                     REGKAITFVNHYEKKYLENVEEYIGYEIRKASLPSDEEVKAGRKIFKESQKVQLKESK
                     KPKKEVHGDIVKIHVNGGKKKKIRAFDIVGCFSNLPGLTGEDIGIIDVQDGFSYVDIL
                     NGKGDKVLKSFKEVTIKGKKVKIQKARK"
     gene            complement(10529..11650)
                     /locus_tag="K8M77_000703"
     CDS             complement(10529..11650)
                     /locus_tag="K8M77_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBI7085219.1"
                     /translation="MKFVLAPDSFKESMTSMEACNAMERGIKKVFKDAECIKVPMADG
                     GEGTVEALVHATFGRIERVNVTGPLGNKVEAFYGVLGNGKTAVIEMASASGIQLVKRE
                     ERNPLITTTFGTGELIKAALDQGIKHLVIGIGGSATNDGGCGMLQALGGKLLDKEGNQ
                     IAFGGGALKDIESIDLSSLDERLKEVSIEVACDVTNPLVGEKGASAVFGPQKGATKEV
                     VKELDNNLAHYASVIKKDLGKEVSHIEGSGAAGGLGAALLLLGGELKRGIELVIKHTE
                     LAEKVKGADFVFTGEGSIDDQTIYGKTPMGVATVAKENGVSTIAFSGKVGEGIEGLYE
                     IGIKSIFSIMTGVLTLDEALKEGSRNLELTTENVVRLLK"
     gene            complement(12042..12557)
                     /locus_tag="K8M77_000704"
     CDS             complement(12042..12557)
                     /locus_tag="K8M77_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBI7085220.1"
                     /translation="MKKGLVVLLSAIVISGALVGCGSDTPKKVEDQNQKQEQQQESKV
                     ETFKVGDTIQTKDFKITVNKVETSEGGEFVKPKDGNEFIKADITIENTSKEEQNVSSM
                     IMFKVVDKDGRSYNQAIVEDQNGQLDGKVAPGRKMTGEYVVEVPKGATGLQLEFDSSL
                     LNSGQVIVDLN"
     gene            12692..13078
                     /locus_tag="K8M77_000705"
     CDS             12692..13078
                     /locus_tag="K8M77_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085221.1"
                     /translation="MNIGKNIRRIRKSKGLTIRDLSKKSEVSQGYISDLENERETRPS
                     LDILGKLANALEVSITNFLEVEIYDELPLANNYRDALIGLTNYINYVTRKNNCELNDI
                     DSEEIVELIESIVLTKIKFYKKKRAR"
     gene            complement(13104..14132)
                     /locus_tag="K8M77_000706"
     CDS             complement(13104..14132)
                     /locus_tag="K8M77_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7085222.1"
                     /translation="MQSRNNNNLKGIDVSNWKGNINFESVKNDGVEVVYIKATEGDYY
                     KDKYAKQNYNGAKEQGLKVGFYHFFRANKNAKEQANYFINYLNEIGATNYDCKLALDI
                     ETTEGIGVRDLTSMCIEFLEEVKRLTRKEVVVYTYTSFANNYIDSRLGNYQVWIAHYG
                     VSTPGANNIWNEWVGFQYSESGSVAGVSGGCDMNEFTEEILIDSNNFNLNNAITKNVD
                     TYLNIRAKGSSSSSIVGTIPAGEKFLIKWVDSNYLGWYYINYNGKTGYVNADFVEKLQ
                     MATTKNVSTFLNVREEGNLNSRIVTKINVGDVFRIDWVDSNFLGWYRITTKNGKVGFV
                     NAEFVKKL"
     gene            complement(14217..14717)
                     /locus_tag="K8M77_000707"
     CDS             complement(14217..14717)
                     /locus_tag="K8M77_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HBI7085223.1"
                     /translation="MKDVLLNQIGPVVIAAVVGIIAVIIKSVGDVVIQLINKKKEEVE
                     QQLELNKHEKEIRVAKEIWNIVEENFRLKDKIEELAKSKADEFDKLLLEKIPYLTKNE
                     VNDIRQAIAGEINKSKAMLNTDDLRKQANDVVTENNKLKSENAELKNKLNAISNYIPA
                     EQVENK"
     gene            complement(14752..15153)
                     /locus_tag="K8M77_000708"
     CDS             complement(14752..15153)
                     /locus_tag="K8M77_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI7085224.1"
                     /translation="MEKIFDCFKWLVITLATGVTWMFGAWDTPLRILILLMILDYISG
                     VLKGYINKELSSDIGFKGIARKAMIFFILILAVMLDRLLNTGTWIFRTITCYFYIANE
                     GISLLENGVVLGVPVPDKLKDALIQLREIKK"
     gene            complement(15213..16718)
                     /locus_tag="K8M77_000709"
     CDS             complement(15213..16718)
                     /locus_tag="K8M77_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085225.1"
                     /translation="MKDAVKFEVPQIQLSGKYKIQLFDKYTNELIEEVEKHNVISKIP
                     FSTAFYNHIYRGFLFNDNNYGCDFSNNKNGYINFLMLTNDPDAPENDDKNPVVLGETI
                     GYAQYDDTNSVEDIKRGIFNKNETKRIFDYYPHGGKIKRVTKHIVFDFGTDKANGTFD
                     NIYLLPTENCSNPDSYRGQQTFFDAFVVSNNEDYKSNSDKYYQGDRGPISHSEKYLYM
                     QCLYYKEVTNRQYWDKIARIDLDTWERKYITLNVPSEEASKPAYIIYACGMFWRIDRE
                     WKTTRYNLDGEYKDTIKLSKSFQKGTYYYLNQYNNFIGDNITTINYNKFMFFTGDDEN
                     LFISYIREDKDKDSKTYYESYILILDKNGEKVNETFFAKYNSLPETPPKLNIAYINNE
                     KYLIANNYTDEKPQVFKVNNGTLIKKSSNILKSVSDFKKPFFYSKDGFLFVVGSGDGG
                     WTYSIYVYTLIPWGSHCKLSSPVTKTSANTMKIQYDVTVDYIMPGMISNLK"
     gene            complement(16731..17729)
                     /locus_tag="K8M77_000710"
     CDS             complement(16731..17729)
                     /locus_tag="K8M77_000710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085226.1"
                     /translation="MSKIENGIYIEDFSESPFFDIKNWQVVNFDNKKILVSNKIGDNS
                     STILEFKVTTNKIEIDYGVSSESNYDKFYIFIDDVEKVCISGLQNKQTLTENLDFGEH
                     KVTFKYSKDSSNGKNRDRGEIFEIRYKIPYKELFLIKDSDSIKGFKDKSLTVLENKIA
                     IKEDFLSYGLSKKELNAIIKNRTPFQSSRPKILFYTDNPKPDIKLYEKGTPHGEIVSM
                     KNSVILDKEYIQYIKDINIEAKLGEDSILKFAISKDDGNSWITFYDNRWVPIGLDNIS
                     LATRGIDLNTLNNLTEDQLKLISDTRKIKLAWYMKRGSLEENIIIKQIKLDYKTNL"
     gene            complement(17729..19552)
                     /locus_tag="K8M77_000711"
     CDS             complement(17729..19552)
                     /locus_tag="K8M77_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085227.1"
                     /translation="MRNVSNDYKAAIRKSSRVVCSKLVFEDENIKTIDDTDIISISLN
                     ESILSGEEFQLGGAIATNIDIEISNYLGRYNEFTFKDLKFKLIFGIELANTNFEYVEY
                     GEYKVFEVKETGKSLKLECYDSFIDMDVPYKTVLQYPQPVHTIISDISKVFNVKLSEK
                     LSSGKYNNSDLIITDRPDDATYRQILKDVAVIVGGFAVIKNGLLDIITIKETDIEINP
                     SNFMKTTITSNYEKLSLIDLNGFTVGKLGGNKLELDIPLLDANDKNEVKKVASSILEI
                     YKEFTYTGFKSDWQGDFSLEVGDSLKVTDINNKEYNSFVASNKISYSGGFKSTTEAKL
                     NKKISKTANREVQRLQNKIKEAENGMYYYCNNNDVNVISAWGQFAFIGFALNGSTNLL
                     LNVAIQVEGTGTLECKIILDSATLGFMPKIKINEFGLLSFTIPLIQLQSSSSHGLSIM
                     LKASDNMIVKGNQGQITIYGDKLSGALSDEYPHAEVKEEIKYDDFYSLQTNLTDNVSV
                     AIEKVINIENSDEVNYTGELFGNASDNINIETVEFGYISDINNYSKSNYEYNTNSIVV
                     DNDGMTIKTLENTVDFIKVNDIYEVDMIDSNTIENIFSEVK"
     gene            complement(19562..19906)
                     /locus_tag="K8M77_000712"
     CDS             complement(19562..19906)
                     /locus_tag="K8M77_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085228.1"
                     /translation="MLVINGVEIATPKIFQVDINDIDGETNRNAKGEMLRDRIATKRK
                     LNCEWPPLSEEECSELLKSVKNIFFQVTYPDPMEGRITKTFYVGDRNVPALSCNDGEI
                     YWKGLKMNFIEK"
     gene            complement(20358..23273)
                     /locus_tag="K8M77_000713"
                     /pseudo
     CDS             complement(20358..23273)
                     /locus_tag="K8M77_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481430.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23317..23679)
                     /locus_tag="K8M77_000714"
     CDS             complement(23317..23679)
                     /locus_tag="K8M77_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085229.1"
                     /translation="MRKRLSDFKKVKNNQWYDLFEDWELIDASFTAQYGIRLRNEPDM
                     SWDEFCTLLSGIMPKTPLGQIISIRSEEDENMLKSFTEEQHKIRNEWRSRQLEEMTDE
                     EKEEQIKELQEILKKAFS"
     gene            complement(23639..23989)
                     /locus_tag="K8M77_000715"
     CDS             complement(23639..23989)
                     /locus_tag="K8M77_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085230.1"
                     /translation="MAKVYNIMNKLICEKPIIKIDENHEYKINNSKNNAIYIQSLVKN
                     KDKKENDIEVLEKIVKASLSKEAFEYIESLDLSLEAYSVIVNAIMAGISNLELDEIEE
                     LNEKEAKRFQESKE"
     gene            complement(24001..24450)
                     /locus_tag="K8M77_000716"
     CDS             complement(24001..24450)
                     /locus_tag="K8M77_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085231.1"
                     /translation="MAFTGVFPVYNLKFKIGTKGKESQSQDMQPIADMENFGIKIDGK
                     VEDWTPMTTSGWARSLMTGKSFSISLKGKRNVGDPGNDYVAATAWKDGLDCSTKGEIE
                     FPDGSKIEFDCVIDVKNVGGDDSTKVAPLEFELKGDGKPTYTPSSEH"
     gene            complement(24460..24846)
                     /locus_tag="K8M77_000717"
     CDS             complement(24460..24846)
                     /locus_tag="K8M77_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085232.1"
                     /translation="MLLSEVREYLKTKIECPQWYIGKIDATKEQCIGIYSIRGPKNHI
                     ALGGLENTNYSTKAISILIHWGKNANIAEQKAQEVFNALFGQEAVIGGKRVIDFDMRT
                     SEPIGVGTDKNNIYEYVIEVNIIHER"
     gene            complement(24846..25229)
                     /locus_tag="K8M77_000718"
     CDS             complement(24846..25229)
                     /locus_tag="K8M77_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085233.1"
                     /translation="MKAKVTIKLDRTKINTLINARNKALEETTEAILSDIKTSAVVPK
                     DTGELERSGFVDLSKLDDGIASIIFDTPYARRLYWHPEYNFRQDKNINAQGKWMQSYI
                     DGDNKEFVTDTYFKFLKMFSKGLIK"
     gene            complement(25229..25555)
                     /locus_tag="K8M77_000719"
     CDS             complement(25229..25555)
                     /locus_tag="K8M77_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085234.1"
                     /translation="MGIKLPFPKWLLKTPVEVYHTYMNEDGEPVEELIYKGLSIYNEK
                     GKNTLDAERRLVTLSGTVTIEGDIYPNKLIEGYIKVGDVKKDIYKSSRPRNPDGSVFS
                     TELELI"
     gene            complement(25559..25921)
                     /locus_tag="K8M77_000720"
     CDS             complement(25559..25921)
                     /locus_tag="K8M77_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085235.1"
                     /translation="MSYVDISYYKNNFKGDILNDDTLENRLERASDQIDKLTYNRIIG
                     IEFKNLSPFQQDKIKKAVCLQAEFIEQYGEFINMPLSGFSAGSTSVSFNGSIVNGITT
                     TQEVINYIYQTGLNSRRL"
     gene            complement(25957..26226)
                     /locus_tag="K8M77_000721"
     CDS             complement(25957..26226)
                     /locus_tag="K8M77_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085236.1"
                     /translation="MKAIKENKVYTITESEQNFYKQQGYDIVNDEGEVIERGAGKSIS
                     YEEYIKLKDELDPLKDENYTLKQENEKLKEENKKLKAENKELKKS"
     gene            complement(26243..27148)
                     /locus_tag="K8M77_000722"
     CDS             complement(26243..27148)
                     /locus_tag="K8M77_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBI7085237.1"
                     /translation="MAVYSYAEQFERELQQKYARELTSYDLEQSNPQVKFINAQTIKL
                     PNITVSGYKDHNRGNMGFNTGTISNEWEPKKLAHDRDIEFALDPMDIDETNLTLEVAN
                     VQNVFETEQAIPERDSYRYSKLYAEAKTYKSNGAVIDNTTLTTENVLDWFDEKMEKMD
                     DEGVPSEGRILYVTPAVNKIIKNAQNIQRSLDVNSNNGKIDRRIYSLDDVTIKKVPSS
                     RMKTKYDFTNGCVPAGDAKQINMILIHPSCQVTRSKYAYMKLFTPGTDSRTADKYVYQ
                     TREYGDTFLIKNKACGIAINAEAEG"
     gene            complement(27172..27780)
                     /locus_tag="K8M77_000723"
     CDS             complement(27172..27780)
                     /locus_tag="K8M77_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085238.1"
                     /translation="MSISNFNLRKRLGMKLAQDDGAGNGGAGSETNSTDNSDGEGERT
                     EETNPKEEKTFTQEEVNRMIKDRVAREKKGQLSKEELKAYQDWKESQKTEAQKQSEAL
                     TNAEKAKQDAEERANTLEAKVTCLSKGVLADNVDDVVILAKAMVSDDVTMDQAVDKVL
                     EKYPSFKGVQQQDENKGFKIGADGGKQKGNVEDALARAFGNK"
BASE COUNT         8656 a         5334 c         2762 g        11147 t
ORIGIN      
        1 aaattctaat aaaaaacaaa agaagctgta taaatatagt taaataaatt aaacttaatt
       61 gtacttagct ttttaataca gctccttttc atataaaaaa tataattatt taatttttat
      121 tttatctttc agttcttaaa gttatggctt cttttgaaga aagctctcca ttattatatt
      181 ttgctgcaat tttaaagtta tatatagtat gtctatttaa tcctttaaat ttataggtag
      241 tctcatcttt tcctaattca gctacttttt taccatcttt atatagcacg tatccttcta
      301 atccaaatga attttcaggt gcgctccact gaacagttac atttttcttt gaaatattag
      361 ttgctttaaa atctctaact gggcttacta taacttctga ggaatcaata gcttttaaag
      421 cttcttctat tacaatcttt aattcaccta aaactttatc cacatctact tttaatgaat
      481 tttcatcatt tttaactctt tcagcctcta aaatagcatc ttctaacttt atttttattc
      541 cttcaatatg atcaactcct ttaagttttt ctaaaacatc atatgctctt tctatctcaa
      601 aatttaattc ttccttatta ggaacttttg aaagttcaaa tttagcttct gcttcagtta
      661 aatcagagat agccttattt atatcctctt gatttgcacc ttcattgttt aatacctcat
      721 tagctttatc taaagctttt ttaagttctt ctatagcatt aggaatatat actccttctg
      781 cattttctaa tttactttct attgaagata ttatctcttt taattgtgat ttatcaatta
      841 cattaactga ttcaacgaaa gtatctatag cttcttttaa agtctttgtt acatttataa
      901 tttcttctaa ggttgtagaa ctttcaaaac cttcctcagc aattcttata gcttcattta
      961 aagcatctac agcttcctta ggatactgtc catttccatt tcccactact gcattttcta
     1021 cttttttctt agctaaaagt atccttgatt tatatgcagt ttcagcactt acagcatcat
     1081 agtatgaatc taatgtattt tttaactcat cataagctgt gtctatttca ctttgtgaaa
     1141 cttgcttttt attgtaaact tctttagcct cattattttc taattttagc ttttgaattt
     1201 ctgcatttac tatagctttt tcattatagt tcatgctatt cataagactt ggtaatattt
     1261 catttagtaa aatatcaaca tcacttatta aagctgcaag tttagttctg tttatttgaa
     1321 caaatccctt atagaattca tttaaggctg attttaaagt gctcataact tcattagcct
     1381 cttttgaatt tatatcattt cctaaagcta attttgcctc atctatagca tttccaaggg
     1441 attccttagc ctcttcagta tagtttccag ccatatcccc aactactgca ctattatata
     1501 tatcttcaac tactcttata taagaagcaa gacttgaagt agaatctcct ttataaattt
     1561 tgatttctcc aattttaaca ttctcactat taaaattaaa tctcacaaat cttcccattt
     1621 cgcctaactt taatatatta gaatatacag ctttattctc ttcattgttg ttctcatcaa
     1681 ctatctttgt ccattctgtt ccatcattag aaatctctat agagtatttt aatggagaat
     1741 ttcctttgct tactatatct attgctgata tatccttttc cttacttaaa tcaaaagtta
     1801 cagacttttc ctcttcttgt cctggaaccc aaagagtatt ttcatttcca tctcttaaat
     1861 tatttgcttc agaattattt gaagttacct caaccttagt ctcatctttc atagctagat
     1921 tttctggaat tgcaaataca tttaattcag cagctgaagc aaatcctcca accccttcta
     1981 aagctacaag tttaacatga gttgcctttg tactattgaa ttttaatgtt tttaaacttc
     2041 caccatcatc ccaatttcct tcagatattt ttctaaaatc atttccatcc tcacttacat
     2101 gaagctcata tttagttata tttccatttt tagcaccact tcttggtaag taagtaaact
     2161 tattaatttc gtaacttcct ccaagttcta aagtaatact ttgtggtaaa ttgtctacag
     2221 gagaatattt tgtatgccat atagtatttt cctttccatc aatagcaaac ttagctaagc
     2281 cttcatttcc tacatcagga tgctctgatg ttgctgtagc tttcatattt ccttgtggta
     2341 atactatgga agcttgctca actttattta tagcttcttt actcatagct ttaatttctg
     2401 caatagctgc ccacgctaaa ggatattctc caccatcagc ttttcctgta atagtagcat
     2461 gtattttact tatttctttt tttacaggaa tattatatga ctttttatta tcctctgtat
     2521 tagaagtcat atccaatact gtttctctat ttccactctc atcttcaact tctactttaa
     2581 atttgaatct aaatccttcc ttttcaaaat gaagttctaa gaagtctaca aaactatttc
     2641 cttctaagtc aattgtggca ttagctggta tagctccatt atcacttatc caaagtgaac
     2701 ttaaatctcc gtctgtaata ttatataaag gatttccact ctgagaatta gaaccactta
     2761 catttttatt ctctgctata tttacatagt tagctggttt ttcttttgac atggccttta
     2821 cttctgcaat agctgcccaa gcccctggat gttgtccacc ttgtgctttt cctgtcatag
     2881 taacatgaac tttatttata tcttttccta caggtatttt atatgatttt tcattatctt
     2941 ctttatttga agtcatatct aatacagttt ctttatttcc atttgcatct tctacttcta
     3001 ctttaaattg gaatctaagt cctagctttt caaagtgaag ctctaagaac tcaacatttt
     3061 ctgttccttg taaatctata gatgtagttg ctggagtttg tccattatta ctaacccaaa
     3121 gagaatttaa atctccatct gtaatattag ataatggttt tccattttca gcttcagctc
     3181 cattaactgg ttttcctaaa gcaacgttag tatactcatc ttccttttct tttcctccat
     3241 ataccattat ttctgctaat tctggtattt tatatgatgg acttaggttt aaatctacat
     3301 tagttaatga aaccctaact ttttgagcct ttccagtaaa gtcaaccttt tcaatgactc
     3361 ccttactaga ataatcctca gatttatctg atactgtttt ccactgtcca ctttcatcca
     3421 taacctctac tttgtatgct acagccctat caccagcctg atttctaaaa gcaaattcta
     3481 ttccatctac acttttaacc ccttgtaaat ctacagttac acttgcagga tatccctccc
     3541 tatcagcaac ccacatagtt cctgtatttc catctgtaat attacttaaa ggattagctt
     3601 cagtttctcc atgagtttca ctaattacag gctttcctaa agcaatatta actctttcaa
     3661 ctgattcatt ttcacctaat attttatcca tattagttac tgaataactt tccatggcta
     3721 tcttagcaag ttctcctaag ttttcattag aagcttctgt aggataaagc ttatctgtat
     3781 cactcttctt gtttacccag tcatactcca tcttaaacca atctatatta ggcgctttag
     3841 ctcctccatc taattcaatt tgtaatccat ttatccattt ttcccatcta gcataataat
     3901 aatccccagt taatcctgac cattgtctat tagaataatc tttaagacca ccaccgtcag
     3961 cattatttct tgaaccccaa gttgttacta aagctctagc attaaattca aataaatcct
     4021 tagtccaatc atcagcgtcc ttaagcatag ttctagcatc ttcaatccaa tttcctatta
     4081 agaattcagg tctagttgat aatactctct cttgaagttt tataagttct agaaattttc
     4141 ctgaaacaaa tttaaatttc tctgcatctc tattattata agcattacac ataacctcat
     4201 aatattcttg agctgagtta gctaataatt gttttaatat atcagcgaaa tcataaagga
     4261 atgcatcact atctttaaat tcatcatagt tctttgaaaa tatttcaata gctttttcaa
     4321 actcactctt atcataaact atttttgaat gtccccaagt tgaagcactt tttattccaa
     4381 atccaggtct tgcatttata atagactcag cagcaccttg gtaataatca tttctctttt
     4441 tgtaagcagt atctaatata atattccaag cctctaaaat ctcttcatta gtcttaccat
     4501 atcttctttc aatataatct tcagtccatg ttctaaagtt tatttgatct ctagtccaag
     4561 ccatatcaaa taaaagttca tatgctaaag gatttgtatt tatagcctct ggtgtaatac
     4621 ctataccaac catatgctct gagttagcta aggctttagg aatttctgtt gcaagttttt
     4681 ctggcgctgc atccattccc attcttcccc caaagttatg aagcatattc caaatccatg
     4741 gtaaatccct ttcctctaat ctattccagt ctggacttac ctctgagaaa agatctaata
     4801 ccatagcttg gtctttttta gttaatcctt ctaacttatt attactagga tttccttgcc
     4861 agttttgtat aacccaaact gcatcattat catgctctat catcttattt tgaattattt
     4921 catatatctt tccattatct aaatcaccag tatttcctcc ctcatggaat ggatctactc
     4981 catagaagtt agttacatct ccaaacactt ctttttgctt ttcatagaaa acatctgcaa
     5041 cattttggaa ataatctacc tccccttcat ttacatatgt tttaagcata tctggtctat
     5101 caaagccaca ccatccccct tgtgaaatag tttgtgcttc tggatttttt tccttaaagt
     5161 ctctaggtac catacctgag tatccttgaa gaacaggatt tattccaaaa ctttgcattc
     5221 tatcatgcat ttttcttcca agctctgctc tttgctcaaa ccaatcgtta ggaagtggac
     5281 ctccaaatcc agtcatattt tgcatataaa accaagcaaa atatgcaggt cctgaaataa
     5341 attctttaac ctcttcatca ctatatccaa attcatttaa ggttcttctt aaaacctctt
     5401 cttgtccaat tatatctaat actaagttaa ccccattcat agcacaccaa tctaggaatt
     5461 cttcatattg atcccaatcc cagaaagcca ttgtataaga gtaagtacaa aagtttaatg
     5521 catatctatg ctcatatgga gtatctataa ctactctctc cccaacagaa ggcattgttt
     5581 caggcatttt taaattagac tccattattg gattgtaact tacattacaa taatttttta
     5641 aataatagtt aaatcctgat gctaaggaaa caccattatt accttttatt aaaacctttc
     5701 caccccttga attagaaacc tcaaaaacat catttccatt taattgatct cttatttcaa
     5761 aaataaactt gtccttaaat ttttctccta ttactctacc aactaaattc tttatttcat
     5821 ttaaagtctt ttcattggca taggcactat cactattaaa cttttcatat tcagtagccc
     5881 aaggggtttc actaaaatta cttgtagcaa ttttcttaac ttccggtaaa ctctctcctg
     5941 tatttttacc aaatacattt atttcagcta tattcaccct atcactatta ctattttgga
     6001 ctacatcaat tctaatcttt cctgccctta cattatcaat ggtatgcaca ttaccattac
     6061 tatctgatac attatcatta tttttatatg ctattttatt aaagttttct ccatcttctg
     6121 aagcatatat attatattta tacacagctt catccttatt aaatatttcg atctttgata
     6181 attcgtaaat tccacctaag tcaacttcta tccatttata atcatttgaa gattcccaat
     6241 aggtagataa atcaccatct atggttaaat cagctgtatt gccctcagta tttccctttg
     6301 cagttacagt aaccccttct gcaatctcaa catcagttga tgccattgcc ttaattgcac
     6361 ttggattaac taatccaaat ataaaagtac ttgttaaaac ataagccatt tgcttcttaa
     6421 attttttact aaacaattta aaatcccctt cactataata tattcaataa aactactttt
     6481 tgttgtgctt tactattata ttaatatgtt tagtaaatta aacaaatgta tgttattaca
     6541 aaaaactaga tgttcctaca aaaaataact aaatatttaa ttctcatctt aaccaaaata
     6601 aacaaaaaat atttttaatc catagtacta aggatttttt ataagaacta aatttttaat
     6661 cactatcact caattaaaag taatagcaaa aattaaagct tatagctaac tttaaactta
     6721 ttaaaattag ctacaagccc taactattta aattaaaata ttttattatt ttttaattat
     6781 tgtggtccca tatatgggtt atttctagtt ttatcttttc caagttttct tgactcttta
     6841 gagtatccta tttcattagc agtttcattt ttcattttct ctacttcttt tttagttttt
     6901 tgactagtct ttttattatc tttgctgttt ttattatttt tatgagacaa agttaaacac
     6961 tcctttcaaa ttaaattctt aattagtatt taactttaat aactcatata tccttaaaac
     7021 aactttccaa aactgcttac tatttcctaa aaaccactaa cactttactt cctcaaattc
     7081 ataatcctta gttacatcat ttacccattt tccactaatt accctatata cactggtaag
     7141 agattttaat atttcatcac attttataac taagaatact aagacaactg gaagttctaa
     7201 aacaaatgct acaaaaaatc cacctggaat agcaacaagc cacataaaaa taagatcatt
     7261 tattaagaca aacttagcat ctccaccacc tctaagtaca cccatcataa aattacttgc
     7321 aagagattga aatactatta ttcctgaagt tacagtcatt atttccatag ctattaattt
     7381 tgttgaataa gatacattat agaaattaac aacaaatggt tttataagta aaatcataag
     7441 tcctgatata catcccatga caacactgaa aatccctata ctataggcat attcctttgc
     7501 tttctctttt ttaccttctc ctatggtatt tcctataatt actgctgttg cattagacat
     7561 accaaatata aaaactgtta caaattgatg agcaactcca tttatactat tagcagctac
     7621 aacctctgtt cccattctac caactattac agatatcata gaagctccaa ttgcccaaag
     7681 taattcatta cataaaacag gagtacataa tccaacatag tcttttaaaa tctctttatc
     7741 tagctttaat aaatgttcta tcttaagacc aatctttctt tcatatataa acataaacac
     7801 taaaactatt gaaaactcag taattctagc acagatagta gctatggcag ctcctcttat
     7861 tccaagttcg ggagcaccta aatttccaaa tataaaaatc cagtttaata ttgaatttac
     7921 cactaaagaa gcagtataaa ctataattga tatactaaca gtttttactg aacgaagcat
     7981 cattattgta cagtttgtaa tagaatagaa caaatatcct atgcacacaa ttcttaaata
     8041 acttgctcca aaatctataa cagccttatc agttgtgaaa attcccataa agtattttgg
     8101 taaaaataac gctataaata tgaaaatacc agttattaat aaacataccc tatacattat
     8161 ggccaaaatc ttatgaattg ttttaacatt tccctttccc caatactgag aaatcataat
     8221 attagatccc ccagctagtc caaacattaa aattgtaagt acaaaaaata aattattagc
     8281 tatagaaact gctgatagtt gaatttcccc caaagctcca accatcaaag tatctaccat
     8341 acttaccata aaggttatta aattttgtac tgctattggc aaagcaatac taaaaagcaa
     8401 cctataaaat cttttgtctc tagttataat cataattagc ccccttacta aaaatatatt
     8461 cttataaaaa tcaatgtaca ttatagcatt ctgtactaac ttataatatg cgtttaaatt
     8521 tagcaatata taaatattaa agcaatttta ttaatatttt tatttttaaa ttaattctta
     8581 attaaaaaaa gccctaagtt ttaaacttag agctttaaaa tctaaactag attatcattt
     8641 gcataataat aatttccatt tataacttaa atgtatttta tcttaaaccg tatagaataa
     8701 ttttattttc ttgctttttg tattttaact tttttacctt ttatagtaac ttccttaaag
     8761 ctctttaaaa ctttatcacc ttttccattt aatatatcaa cataagaaaa accatcttga
     8821 acatctatta ttccaatatc ttctcctgtt aaacctggta agttactaaa acatccaaca
     8881 atatcaaagg ctcttatttt ctttttcttt ccaccattaa catgtatttt aactatatca
     8941 ccatgaactt ctttcttagg tttcttactc tcttttaatt gaaccttttg actttcctta
     9001 aatatttttc ttccagcttt aacttcttca tcagaaggaa gacttgcttt tcttatttca
     9061 tagccaatat attcttcaac attctctaaa tatttcttct cataatgatt tacaaaggta
     9121 atagcctttc cttctcttcc tgctctacca gttcttccta ttctatgaac ataactttct
     9181 gtttccattg gaacttcata gtttataaca agctctatac tatctacatg aattcctcta
     9241 gcagctatat cagtacaaac taatacttta aagtgcttat ctctaaagtt ttccataacc
     9301 tcaatacgct tcttttgctc catatcccca tgaagttcat tacaagttat tccctcatcc
     9361 ttaagcattt tataaacaga cttaacctta tttcttgtat tacaaaatac tatagcactc
     9421 tctggatgat aagcataaag gcatttccat aaagctttcc ccttattttt ctcctctatc
     9481 tcaattgcac tttcttctat tctatcccta ttaaatactt ttgatttaac ctctaaaagc
     9541 ataggatttc tcatatactc ttcacatatt tcctctattt ccttagaaaa tgtagcagaa
     9601 aataatgcag tggttttgtt taatcttaat tctcctaata tgtcctttat ttgatcaata
     9661 aatcccattg aaagcatttt atcagcttca tctatgacta catagttaac ttcacttaag
     9721 tctaaagttc ctctttccat atgatcaaat actcttcctg gagttcctac tactatatgt
     9781 actctttgtt taagctctct aatttgctca ttaaaaggtt gtttcccaaa tatagccgca
     9841 catctaacct tttttattct tcctatatta gaaagttcat ctttaacttg aattgctaac
     9901 tctctagttg gtgttaaaac tagacactga accttttttt cttcaatatt tatcttttca
     9961 caaacaggta ttccaaaact tgcagtttta ccactaccag tttgagattt aactattata
    10021 tcttcaccct ttaataactt tggaatagaa gcttcctgaa cctctgatgg atttttatat
    10081 cctaaattat taagtgcttt aataatatcc tcacttaaat ttaattcatt aaatttcata
    10141 tattgttttt atctcctttt ttaaaacttt attagtcttc ttatagttat accatatatt
    10201 cattttttct actaatttta atttttaaaa tcctaagaaa ataagataac attacatttt
    10261 tccatatatc ataaagaggg aaacagttaa aattcttaaa attaaagaaa taatataata
    10321 ctatgttttc tcctttatta acttatatat tttaaaatat cttaattata aagaattttc
    10381 tttataataa aaaagtgaac acaaacaata tattttttat tgcttatgtt cactttaaac
    10441 tatctcttgt aagcaagtat taaattttct ttaattcttt atcttcactc tatcacttta
    10501 taaatataac tttataaagt cataaacttt attttaaaag tctaactaca ttttcagttg
    10561 ttaattctaa atttctactt ccttctttta aagcttcatc taaagttaaa actccagtca
    10621 ttatactaaa tatactcttt atacctattt cataaaggcc ttctatcccc tctccaactt
    10681 ttcctgaaaa ggcaattgta gaaactccat tctccttagc tacagtggca actcccattg
    10741 gtgtttttcc gtatatagtt tgatcatcta tacttccttc accagtaaat acaaagtctg
    10801 ctccctttac cttttcagct aactctgtat gttttattac aagctctata cctcttttta
    10861 attctccacc taatagtaat aaagcggcac ctaatcctcc agcagcacca gaaccttcta
    10921 tgtgtgaaac ttcttttcct aggtcttttt ttattactga agcgtaatga gctaaattat
    10981 tatctaattc tttaaccact tctttagttg ctcccttttg agggccaaat actgctgagg
    11041 ctcctttttc tcctactaat ggattagtta catcacaagc tacttctatt gatacttcct
    11101 ttagtctttc atctaaagaa cttaaatcaa tactttctat atcttttaat gctcctccgc
    11161 caaaggctat ttgatttcct tccttgtcta aaagttttcc acctaaggct tgaagcattc
    11221 cacatcctcc atcattagtg gcacttccac ctattcctat aactaagtgt tttattcctt
    11281 gatctaatgc agcctttata agctctccag ttccaaaagt tgttgttatt aaaggatttc
    11341 tttcttctct cttaacaagt tgaatcccag aagcactagc catttctata acagcagtct
    11401 ttccattacc taaaactcca taaaaagctt ccaccttatt tcctaaaggt ccagtaacat
    11461 ttactctttc tattcttcca aaggtagcat gaactaaagc ttctacagtt ccctctccac
    11521 cgtcagccat tggaaccttt atgcattctg catctttaaa gaccttttta attcctcttt
    11581 ccatagcatt acaggcttcc attgaagtca tactttcctt aaatgaatct ggtgctaata
    11641 caaatttcat ctttatttcc tcctactaga ttttatctta atctttcatt ttaaaatcca
    11701 ctgtgctttt actaaaaact ctattttaaa tcataattct taaaaattaa taaatttcat
    11761 accaagttta aattaaacct tattaaaaat tttctttatt ataaagtaac aaaatattta
    11821 taccttagcg ataataacta tatagttaaa acttttcttt atttattaaa taactatttt
    11881 aatattattc caaatattat agttgataca attgtcattg ttaatccaac taaagattca
    11941 tatggtataa gtttaagtct ctctttcata tccattgaaa cgcttcctat aatgtttatt
    12001 ttaacaaaaa aagactagga tttcctagtc ttttttactt cttaatttaa atcaacaata
    12061 acttgtccgc tatttagtaa tgaactatca aattctaatt gtaatccagt agctccctta
    12121 ggtacttcta ctacatactc tcctgtcatt tttcttcctg gagctacttt cccatctaat
    12181 tgaccgtttt ggtcctcaac tatcgcttga ttatatgatc ttccatcttt atctacaact
    12241 ttaaacataa tcattgatga aacattctgt tcttctttag aagtattttc gattgttatg
    12301 tcagctttta taaattcatt accatcttta ggtttaacaa actctccacc ctcagatgtt
    12361 tcaactttat taactgttat cttaaagtct ttagtttgta tagtatctcc aactttaaaa
    12421 gtttctactt tactttcttg ttgttgctct tgcttttgat tttgatcttc taccttttta
    12481 ggagtatcac ttccacaccc tactaatgct cctgaaataa ctatagcact taataaaaca
    12541 accaatcctt ttttcattat tttatcctcc tagttcttta ttcattaaca ttatataaca
    12601 ttattgtaaa tttaaaaagt ttttactttt taaatactta gctatattct ttatttaaga
    12661 tgttataata taaaaatagt gaggtgttaa catgaatata ggaaaaaaca taagaagaat
    12721 tagaaaatca aaaggtctta caattaggga tttatctaaa aaaagtgaag tttcccaagg
    12781 ctatattagt gatttagaaa atgagagaga aacacgacca tcacttgata tattaggtaa
    12841 attagcaaac gctctagaag tgtcaataac taacttttta gaagtagaaa tctatgatga
    12901 gcttccttta gctaataatt atagagatgc tctaattgga ttaactaatt atataaatta
    12961 tgtaactagg aaaaataatt gtgaacttaa tgatattgat tcagaagaaa tagttgaatt
    13021 aattgaaagt atagtcctta ctaaaattaa attttataaa aaaaaaaggg ctaggtagga
    13081 attaccccac ctagcttttt taactataat tttttaacaa attcagcatt aacaaatcca
    13141 acttttccat ttttagttgt tattctatac caacctaaaa agttactgtc tacccaatca
    13201 attctaaata catcaccaac atttattttt gtaactattc ttgaatttaa atttccttct
    13261 tctcttacat ttaaaaatgt acttacattc ttagtagtag ccatttgtaa tttttcaaca
    13321 aaatctgcat taacatatcc tgttttacca ttatagttta tgtaatacca tcctaaataa
    13381 tttgaatcaa cccattttat taagaatttc tcaccagctg gtattgttcc gacaatacta
    13441 gaactagaac ttccttttgc tcttatatta agataggtat caacattttt tgtaattgca
    13501 ttgtttaaat taaagttatt agaatcaatt aatatttctt ctgtaaactc attcatatca
    13561 catccaccac ttacaccagc tacgcttcca ctctctgaat attggaatcc aacccattca
    13621 ttccatatat tattagctcc tggtgtagac actccataat gtgcaatcca aacttgataa
    13681 tttcctaatc tgctatctat atagttattt gcaaaacttg tataagtata aaccactact
    13741 tcttttcttg taagtctttt aacctcttct aaaaactcta tacacataga agttaaatcc
    13801 cttaccccta taccttcagt agtttctata tctaaagcta atttacaatc atagttagtt
    13861 gctcctattt catttaaata attaataaaa taattagctt gttctttagc atttttattt
    13921 gctctaaaga aatgatagaa tcctaccttt aaaccttgtt cttttgctcc attatagttt
    13981 tgtttagcat atttatcttt gtaataatca ccttctgtag ctttaatata aaccacttct
    14041 acgccatcat tttttacact ctcaaaattt atatttcctt tccagtttga tacatcaatt
    14101 ccttttaaat tattattgtt tctactttgc attattaatt ccttctttct tgaaattatt
    14161 tataattaaa gacaataaaa aaaggctatc tctaacctaa tctattgcct tagtttctat
    14221 ttattttcta cttgctctgc tggtatgtag ttagaaattg catttaattt attttttaat
    14281 tctgcatttt cagattttaa tttattgttt tctgtaacaa catcattagc ttgttttctt
    14341 aaatcatctg tatttaacat agctttactt ttgtttattt caccagctat agcttgtctt
    14401 atatcattta cttcattttt tgttagatat ggtatttttt ctaataataa tttatcaaat
    14461 tcatctgcct tgctttttgc taattcttcg attttatctt ttaatctaaa attttcttct
    14521 actatattcc aaatttcttt tgcaactcta atttcttttt catgcttatt taattctaat
    14581 tgttgctcta cttcttcttt ctttttatta ataagctgaa ttactacatc tcctacactt
    14641 ttaatgatta ctgctattat tccaacaact gctgcaataa caactggacc aatttgattt
    14701 aataatacat ctttcatttt aattacctct ttcttataat ttattttagt tttatttttt
    14761 aatttctcta agttgtatta aggcatcttt aagcttatca ggaactggaa ctcctaatac
    14821 taccccattt tctaataagc ttataccttc atttgcaatg tagaagtagc atgttattgt
    14881 tctgaatatc caagttcctg tatttaaaag cctatctaac attaccgcta atattaaaat
    14941 aaaaaatatc atagctttcc ttgctattcc tttaaaacct atatcgctgc ttaattcttt
    15001 atttatatat ccctttaata caccacttat ataatcaagt atcattaata aaattaatat
    15061 ccttaacggt gtgtcccatg caccaaacat ccaagtaact cctgtagcta atgtaattac
    15121 tagccattta aaacaatcaa aaattttttc cattaatcaa tccttctttc ttaatttttc
    15181 gcataaaaat agagctaaca attaagttaa ctctatttca aattagaaat cattccaggc
    15241 attatataat ctactgttac atcatattga attttcatag tgtttgctga agtttttgta
    15301 actggtgatg acaacttgca atgagacccc caaggaatta acgtatatac atatatacta
    15361 tatgtccaac ccccatctcc agatcctacc acaaataaaa aaccatcttt agaataaaaa
    15421 aatggttttt taaagtctga tacagattta agtatattac tacttttttt aattaacgtt
    15481 ccattattaa ctttaaatac ttgaggtttt tcatcagtat agttattagc tattaaatat
    15541 ttttcattat ttatatatgc tatatttaac tttgggggag tttcaggtaa tgaattatat
    15601 ttagcgaaaa aagtttcatt taccttttca ccatttttat ccaatataag tatataagac
    15661 tcataatatg tttttgagtc tttatcttta tcttctctta tataagaaat aaaaagattc
    15721 tcatcatcac cagtaaaaaa cataaattta ttataattaa tagtagtaat attatcacca
    15781 ataaaattat tatattgatt caaataataa tatgttccct tttgaaaact ttttgatagt
    15841 tttattgtat ctttatattc accgtctaaa ttatatctag ttgttttcca ttctctatct
    15901 attctccaaa acataccaca tgcataaatt atatatgctg gtttagaagc ttcttcgcta
    15961 ggtacattta aagttatata tttcctttcc caagtatcta agtctatcct agctatctta
    16021 tcccaatatt gtctattagt aacctcttta taatataagc attgcatata taaatatttt
    16081 tccgaatgag aaattggtcc tctatcgcct tgataatact tatctgaatt tgatttataa
    16141 tcttcattat tagacacaac aaatgcatca aaaaaagttt gttgccctct atatgaatct
    16201 gggttcgaac aattttcagt tggtaataaa taaatattat caaaagtacc atttgcttta
    16261 tctgttccaa aatcaaaaac tatatgtttt gttacccttt taattttacc tccgtgggga
    16321 taatagtcaa atattctctt tgtctcattc ttattaaata tacctctttt tatatcttca
    16381 actgaattgg tatcatcata ttgggcataa ccaattgttt ctcctagtac aactggattt
    16441 ttgtcatcat tttctggagc atcaggatca ttagttaaca taagaaaatt tatataacca
    16501 ttcttattat ttgaaaaatc gcaaccataa ttattatcat taaataaaaa tcctctgtat
    16561 atatgattat aaaaagctgt agaaaaaggt atcttgctaa tcacattatg cttttcgact
    16621 tcttctatta attcatttgt atatttatca aataattgta ttttatattt accacttaat
    16681 tgtatttgtg gcacttcaaa tttaactgca tccttcatat tattcaccac ctataaatta
    16741 gttttataat ctagttttat ttgttttata attatatttt cttctaagct tcctcttttc
    16801 atataccacg ctaactttat ttttcttgta tcagatatta actttaattg atcctctgtt
    16861 aaattattta aagtatttaa atctattcct ctagtagcca aagaaatatt atctaaacct
    16921 attggcaccc atctattatc ataaaaagta atccatgagt taccatcatc tttagaaata
    16981 gcaaacttta atatactatc ttcaccaagc tttgcttcaa tatttatatc ttttatatat
    17041 tgaatatatt ctttatctaa tataacacta tttttcatac tcactatttc accatgcgga
    17101 gtaccttttt cataaagttt tatatctggc ttaggattat ctgtataaaa taaaatttta
    17161 ggtctactac tttgaaaagg tgttctattc tttataattg catttaattc cttcttgctt
    17221 aatccgtaac ttaaaaaatc ttctttaatt gctattttat tttctagtac tgttagtgat
    17281 ttatccttaa aaccttttat tgaatcacta tcttttatta agaataactc tttataagga
    17341 attttatatc taatttcaaa tatctctcct ctatctctat tcttaccatt gctgctatct
    17401 tttgaatact taaaagttac tttatgctct ccgaaatcca agttttcagt caatgtttgt
    17461 ttattctgca aaccacttat gcatactttt tctacatcat ctatgaatat ataaaattta
    17521 tcataattac tttctgaact aacaccataa tctatctcta ttttattagt agttacttta
    17581 aattctaata ttgtactaga gttatcacct atcttattgc taactaatat ttttttatta
    17641 tcaaaattta caacttgcca atttttaata tcaaaaaaag gactctcgga gaagtcctct
    17701 atatatattc cattttcaat tttactcatt atttaacctc actaaaaata ttttctatag
    17761 tattactatc aatcatatca acttcataaa tatcattaac ttttataaaa tcaacagtat
    17821 tttctaaagt tttaatggtc ataccgtcat tatcaacaac tatagaatta gtattatatt
    17881 catagtttga tttagaataa ttattaatat ctgatatata tccaaattca actgtttcaa
    17941 tattaatgtt atcagaagca ttaccaaata attctccagt ataatttact tcatcagaat
    18001 tttctatatt aataactttc tcaatagcaa ctgatacatt atcagttaag ttagtttgca
    18061 atgaatagaa atcatcatat ttaatttctt ctttaacctc ggcatgagga tactcatcag
    18121 ataatgctcc acttaactta tcgccgtata ttgttatttg tccttgattt cctttgacaa
    18181 tcatattatc agaagcttta agcattatac ttagcccgtg actagaacta ctttgtaact
    18241 gaattaatgg aatagtaaaa gataataaac caaattcatt tatttttatt ttaggcataa
    18301 atccaagtgt ggctgaatct agtattatct tacattctaa tgttccagtt ccttctactt
    18361 ggatagctac attaagcaaa agattagtag atccattaag agcaaatcct ataaaagcaa
    18421 actgtcccca tgctgatatt acattaacat cattgttatt acagtaatag tacataccat
    18481 tttcagcttc tttaatttta ttttgaagcc tttgaacttc tctattagct gttttactaa
    18541 tttttttatt taactttgct tccgtagtag atttaaagcc accagaataa gaaattttat
    18601 tactagcaac aaatgaatta tattctttat tattaatatc agttacttta agagaatctc
    18661 ctacctctaa agaaaagtct ccttgccaat ctgatttaaa acctgtataa gtaaattctt
    18721 tatatatttc taaaatgctt gaagcaacct ttttaacttc atttttatca ttagcatcaa
    18781 gtaagggtat atctaactct agcttatttc cacctaattt acctacagta aaaccattta
    18841 agtctattaa agataatttc tcatagttac ttgttattgt tgttttcata aagttactag
    18901 gatttatttc tatatctgtt tctttaatag ttattatatc taataaacca tttttaatta
    18961 cagcgaatcc tccaactata acagcaacat cttttaatat ttgtctataa gtagcatcat
    19021 ctggtctatc tgtaattatt aaatcagaat tattatattt tccactagaa agcttttcac
    19081 taagcttaac attaaatacc ttactaatat cacttattat tgtatgtact ggttgagggt
    19141 attgtagtac cgtcttataa ggcacatcca tatcaataaa gctatcataa cattcgagtt
    19201 ttaaactttt accagtttcc ttaacttcaa atactttata ttccccatat tccacatatt
    19261 caaaatttgt attagctaat tcaattccaa atattaattt gaattttaaa tccttaaatg
    19321 taaactcatt atacctacca agataattac ttatttcaat atcaatatta gttgctatag
    19381 ctccaccaag ttgaaattcc tctccactta gaatagattc atttaaagat atagaaataa
    19441 tatcagtatc atctatagtt tttatatttt catcttcaaa aacaagctta ctacaaacaa
    19501 ccctactact tttccttata gctgctttat agtcattaga tacatttctc atagaactca
    19561 cctacttctc gatgaaattc atctttaaac ccttccaata aatctcacca tcattacaag
    19621 ataatgcagg tacatttcta tcgcctacat aaaatgtttt agttattcta ccttccattg
    19681 gatccggata agtaacttga aaaaatatat ttttaacact ctttaataat tcggaacact
    19741 cttcttcact taaaggtggc cattcacaat taagttttct tttagttgct attctatctc
    19801 ttagcatttc acctttagca tttctattag tttcaccatc tatatcatta atatcaactt
    19861 gaaaaatttt aggagtggct atttccactc catttattac taacatctaa ccactcctat
    19921 atatttaata tttgttttcc tgtctttctt tctaatcttc taaaagcttt tattattaaa
    19981 tcacatattt tatcatcacc aacgattaac tctaagttta aatcaccatt atagttatta
    20041 ttattattgt tatcgccatt tttgtttatt ccttctttta aagcttctat aatagcctct
    20101 tttaattttt ctatgtaatc ataatccatt ttagaatcat tcgatacatt ctgtcctaac
    20161 atagttaaat taggctgttt tattatgtta ggtatactaa ccatcttact tattctttca
    20221 gataatatat tagctaataa attcaatcca ccagtattat tttctaatgg tactacggcc
    20281 tctgttccag cttcacccac tacagcttgt gtaggcttat caactatacc acctttagct
    20341 agataaggaa tcttaggtaa attaacacca aaatgttttc cacctaatcc acctggcaac
    20401 caatctggag ccgtaaagct tattttattt aatccactta tagccgcatt aactaatcca
    20461 ataacggtat taagtggcgc tttaattacg gcacctaatc cattcattat tccaccaaat
    20521 atatttacaa ctccttgcca tgctctgctc caatttcctg taaatatacc tgtaataaaa
    20581 tctataattc cgccaaatat tctttttata gcattccatt tattttcaac attcctcata
    20641 aaggcattta taatattgcc aaaaactcca aaagatttag tccaatctgt agtaaataat
    20701 cctgtaagga aattatcaaa ctttgtaaag actgatttta tagaagccca aacattatta
    20761 actccatttc taaaccaatc acatttatta tataaagtta caaatacagc agctaaacct
    20821 aaaagtaatc caacaacaag agtaattgga ttcatagaca tagctaagtt aagagctttc
    20881 tgtgctatag tcatagtatt agtagctaaa gctactgctt tagttgcgac aacatcagct
    20941 aatttagcac ctttactaat aacaaattgt gctatttgtt ttgaaatttc tgctgttcct
    21001 ttagcaatag atacagcaaa atctttagca tacattaaag ttaatttagc agtttctaac
    21061 ttatcagcta tcttagttaa agtacacgcc ttcaaagctg tacccattgt atttataaca
    21121 cctataactc caccagcctg ttgcataaaa gccattagtt tagccacctt ccaagctgtg
    21181 aaaaatgctg ttactgtaac tatcattata tctaaaactg gttttccatt tcctaataac
    21241 caacctatta aattactaaa tgcatctaaa acacttccta caatatctgc taatttagct
    21301 acaactggag atataagatt tacaaaccag ttaatcatag gagcaacaaa attagtataa
    21361 atatatcctg ctaattcaaa aattttagca cctaatctta caaagccttg gaataaatga
    21421 cttccaccat tatcccaaac ataaactaat ttttgagtta agttttctaa aactcctgct
    21481 gttgcattta agacttgcat aaatgttgct gctatacctg ggcctacttc tccccatact
    21541 tctcttaaag aatctcccat atgcttaatt aatgtaagaa catttagaaa agcattagct
    21601 aaagcttgaa caatggctgt tcctattcca ccagcattcc aggcatctgc aaatgtaata
    21661 cctatatctc ctatgagatt aaatatattc tgtagtattt gtaatatggt agttaaaatt
    21721 gcctcaccag ttccattagt ccaaacttcc atgaagctat gacctattgc acctaataat
    21781 tcctttattc catataaagc gtgttttata ctttctatag ttgcagctcc ttcactagcc
    21841 catgcatttt taaaaggttg aaatattctt gaaagtgtat cttttaattt atcaaccata
    21901 ccattaattg tttgcatagc tgcacttgtt gggcttaagt caacattagg agctaataat
    21961 ggtataggtt ttatccctcc acttttttct tttggttctt ttggaccttt taatttatta
    22021 atttcatcga atccagctaa tgtaccttta gattttttag cagctttacc aattccattt
    22081 acagcattag aagttttttt actttgcttc tccatatttt tcattgctgc tatggaagca
    22141 ttcatacttt gagctgctcc aaaactagct tgatatgttt taccaaatat agcatttata
    22201 aaagctgcaa tatatgctgt tacatttgct aatgcactca ttaaagcatt taacgcaggt
    22261 aatatgtatt catatattgg cataaatgct actattagat tagttcttat ttgaattaaa
    22321 ctatttgcaa actgtgcatt tgtcattaat gcactaccaa tataacctcc aactgctgaa
    22381 atacctttca ttataatagg aaatactatt ccccatttaa acatactatc tataaacatt
    22441 cctgttgcac ttctagcacc atttatattt tctctatatt gtctagttga attattggta
    22501 ttccttaacc ttctgcttac tctgctagta gcattttcaa gcctcttcat gctattactt
    22561 gcttcattta atcctatagt agagttctta gatgcatttc ctaatctttc aaactctcta
    22621 tccaaatctg ctaatttaaa tccagttgca tcagatttag ctataagctt atttataaca
    22681 gcttctgtct ttaatatttg ttcttgaatt ttattttttc tagcttgatt aaaagttgaa
    22741 ttgtatgatt cttttaaccc tgccaatttt tcttgctgtt gctctattct cctatttgta
    22801 atctctaaac tattagataa attatcaatt ttagctttaa gtgactctaa attaatagaa
    22861 gaactttttg gtggtcctct aggttttaaa actttttgat aagtttgact tggtgtagca
    22921 atattttgaa ctggtgaaaa gtttaatggt atttttattt tctttgcatt agcaacaata
    22981 gatcttatag catctaatgc cttttccttt atctcttcaa tagattttaa aatcgtagct
    23041 ttattcttct ctacattaga ttttattgtt tcatttatgg aatttaatgc accatttaaa
    23101 gcttctgaaa taccattaga tatattttca aaatttaaat ttttaattga attctctaat
    23161 ttttttccta taccttctga tattttatta gcaaattgtt caatctgttt atctatatca
    23221 ccagtaatct ctatatcaag accaatcttc ccaactgaat cttcatctgc catttacaac
    23281 tcaccacctt tctcaaaata aaaaagacac ttagctttaa ctaaatgcct ttttaagaat
    23341 ttcttgtaac tcttttattt gttcctcttt ttcttcatct gtcatttctt ctaactgtct
    23401 acttctccac tcattacgaa tcttgtgttg ttcttctgta aaactcttaa gcatattttc
    23461 atcttcttca cttcttatag agattatttg acctaaagga gttttaggca ttattccact
    23521 aagtaaagta caaaattcat cccaactcat atctggttca ttcctaagtc ttataccata
    23581 ttgagctgta aaagaagcat ctatcaattc ccaatcttca aatagatcat accattgatt
    23641 attctttact ttcttgaaat cgcttagcct ctttctcatt tagttcttct atttcatcta
    23701 actctaaatt agatattcca gccattatag cattaactat tacactatat gcctctaagc
    23761 ttaagtctaa actttctata tattcaaaag cttctttaga taatgaagct tttactatct
    23821 tctccaaaac ttcaatatca ttttcttttt tatccttatt cttgactaat gattgaatgt
    23881 atatagcatt atttttactg ttatttattt tatattcatg attctcatct atttttataa
    23941 ttggtttttc acatattaac ttattcatta tattatatac ttttgccatt attatacctc
    24001 ctaatgttct gatgatggtg tatatgttgg tttcccatca cctttaagtt caaactctag
    24061 tggagcaacc tttgtactat catctccacc aacgttttta acatcaatta cacaatcaaa
    24121 ttcaatcttt gagccatctg gaaactctat ttctcctttt gtactacaat ctagtccatc
    24181 tttccacgct gttgctgcaa cataatcatt tcctggatca ccaacatttc tttttccttt
    24241 taaagatata gagaaactct ttcctgtcat taaacttctt gcccaccctg atgttgtcat
    24301 aggtgtccaa tcttctacct taccatctat tttaatacca aagttctcca tatcagcaat
    24361 aggctgcata tcttgagatt ggctttcttt tccctttgta cctatcttaa atttaagatt
    24421 atatactgga aatactcctg taaatgccat aattattcac tacctttcat gtattatatt
    24481 tacttctatt acatattcat atatgttatt tttatctgtt ccaactccta taggttcgct
    24541 agttctcata tcaaaatcta taactctttt cccacctata acagcttctt gaccaaataa
    24601 agcgttaaat acttcttgag ctttttgttc tgctatatta gcgttctttc cccaatgtat
    24661 tagtatagaa atagccttag tagaatagtt tgtattttct aatccaccta aggctatatg
    24721 attttttggc cctcttatgc tatagatacc tatacattgc tctttagttg catctatctt
    24781 tcctatatac cattgagggc attctatttt agtttttaaa tattctctta cttcacttag
    24841 caacattatt taatcaatcc tttgctaaac atttttaaga atttaaaata agtatctgtt
    24901 acaaattctt tattatctcc atcaatataa gattgcatcc atttaccttg tgcatttata
    24961 tttttatctt gtctaaaatt atattctgga tgccaatata atcttctagc atatggtgta
    25021 tcaaaaatta tagatgctat tccatcatct aacttcgata aatcaacaaa accacttctt
    25081 tcaagttctc cagtatcctt aggaactaca gcacttgtct taatatcact taatatggcc
    25141 tctgtagttt cctctaaagc tttatttcta gcatttatta aagtgtttat cttagttcta
    25201 tctaatttta ttgttacttt agccttcatt agataagctc caattcagta gaaaatacac
    25261 ttccatctgg attacgtggc cttgatgact tataaatatc ttttttaaca tctccaacct
    25321 ttatatatcc ttcaattaat ttattaggat aaatatcacc ttcaattgtt acggtaccac
    25381 ttaaagttac aagtctacgc tctgcatcga gagtattctt tcccttttca ttatatatac
    25441 ttaatccttt ataaattaac tcttctactg gttcaccatc ctcattcata taagtatggt
    25501 aaacctctac tggtgttttt aaaagccatt taggaaatgg taatttaatt cccatagttt
    25561 agagcctcct actatttaaa ccagtttgat atatataatt aataacttcc tgtgtggttg
    25621 ttattccatt tacaatactt ccattaaaag atactgaagt actaccagct gaaaatccac
    25681 ttaaaggcat attaataaac tcaccatatt gctctataaa ttcagcctgt aaacaaactg
    25741 cttttttaat tttatcttgt tgaaatggag ataaattttt aaattctatt cctattattc
    25801 tgttatatgt caatttatca atttgatctg atgctctttc taatctattt tctaaagtat
    25861 catcattaag gatatcacct ttgaaattat ttttataata tgaaatatct acataagaca
    25921 tatactcaca tccttataaa gaaaaagaag ccttaactaa gacttcttta actctttatt
    25981 ttcagcttta agctttttat tttcttcttt taatttctca ttttcttgct ttaaagtata
    26041 gttttcatct tttaatggat ctaattcatc ttttaacttt atatattcct cataagaaat
    26101 agatttccct gctccacgtt ctataacttc cccttcatca ttaactatgt catatccttg
    26161 ttgtttataa aagttctgtt cagattcagt tattgtataa actttatttt ctttaatagc
    26221 cttcatatgt tattactcct ttctagccct ctgcctctgc attaatagcg ataccacatg
    26281 ctttattttt tattaagaaa gtatctccat actccctagt ctgatacaca tatttatcag
    26341 ctgttcttga atcagtacca ggagtaaaca acttcatata agcatattta cttctagtta
    26401 cttggcaaga tggatgtata agtatcatat tgatttgttt tgcatcacct gccggaacac
    26461 atccattagt gaaatcatac tttgttttca ttcttgatga tggtactttt ttgattgtaa
    26521 catcatctaa agaatatatt cttctatcta tctttccatt attgctatta acatctaaac
    26581 ttctttggat attttgtgca ttcttaatta ttttgttcac tgctggtgta acataaagga
    26641 ttcttccttc tgatggtact ccctcatcat ccattttctc catcttttca tcaaaccaat
    26701 ctaatacatt ttctgtagtt aaagttgtat tgtctataac agctccattt gatttatatg
    26761 ttttagcctc tgcataaagc ttagagtatc tataagaatc tctctctgga atagcttgtt
    26821 ctgtttcaaa tacgttttga acattggcca cttctaaagt taaattagtt tcatctatat
    26881 ccataggatc taaagcaaat tctatatctc tgtcatgagc taattttttt ggttcccatt
    26941 cattagatat tgttcctgta ttgaaaccca tattacctct attgtgatcc ttgtatccac
    27001 ttactgttat attaggtaat ttaatagttt gtgcattaat gaatttaact tgtggattag
    27061 attgctctaa atcataagaa gttaattctc tagcatactt ttgttgtaat tctctttcaa
    27121 attgttcagc ataactatat actgccattt atatcactcc taatcttttt attatttatt
    27181 tccaaaggct cttgctagtg catcttcaac atttcctttt tgttttccgc catcagcacc
    27241 tattttaaag cctttgtttt catcttgttg ctgcactcct ttaaagcttg gatatttttc
    27301 taaaacttta tctactgctt gatccatagt aacatcatca cttaccatag ctttagctaa
    27361 tataacaaca tcatcaacgt tatcagctaa aacaccttta gataaacaag ttacttttgc
    27421 ctctaatgta ttagctctct cttctgcatc ttgtttagct ttttcagcat tagttaaagc
    27481 ttcactttgc ttttgagcct cagtcttttg actttctttc caatcttgat aagctttaag
    27541 ttcttcttta gatagttggc ctttcttttc tcttgccacc ctatccttaa tcatcctatt
    27601 tacttcttct tgagtaaaag tcttttcttc ttttggatta gtttcttctg ttctctcacc
    27661 ttctccatct gaattatcag ttgaattagt ttctgaacct gctcctccat tgcctgctcc
    27721 atcatcttga gctaatttca tacctaatct ttttcttaaa ttaaaatttg atatagacat
    27781 aaatacctcc atttatagcc tatcggctat taattccatg cacagtttaa agtcttaagc
    27841 aagttttgga caaaataaaa aagtggctag atttcaacca cttttaatat ttataatta
//
LOCUS       DAEVGI010000018        27814 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEVGI010000018 DAEVGI010000000
VERSION     DAEVGI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 27814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27814
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.018"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(85..1911)
                     /locus_tag="K8M77_000724"
     CDS             complement(85..1911)
                     /locus_tag="K8M77_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7085239.1"
                     /translation="MGIKLTSSFSRWWKNYSLSVRSIENMFIVIFAVFIPVLYIILFG
                     GYSFDNIAKLNSKANPDIFNRVIYRDALINPHKYKECRLLAQSVDISDFLYNETNYLS
                     KLDSLETKEQKQTFITKLQERATSYSGVVLINKKTGEYYSNRVWFYEKPYNLSTPKEV
                     LESLSKNDNVVFKEINSLDNYEEIYFSRGELYDSEINSIRIAFIATMTTTLLMLLLII
                     KKLVMIKNMGIRAYKESWKNGYFFRFLDSLNVLITKKMFIEEFLKDKVVMFVISFAII
                     YILINGFMRNIEFVWKQYHYVLTDNRYLWIIPIVLVVHFIVKFIRKYDGLDNVIRGIE
                     KVKKGDTDIEIKDNDDRQVRELADDVNDLRLGYKELVEEGIKNEKLKTELISNVSHDL
                     KTPLTSIINYVNILQLNNIKEEERKEYIEILDKKSQTLKKLIDDLFEVSKMSSGKVEL
                     FKYNIDIIQLVYQCIAEVEDVYSEKEVEFKINGPEECMVKIDPQRMSRVFQNLITNAL
                     KYSLEKTRVYVEIKDLENYLEISFKNISSYALDFDEKDILERFARGDESRNSTIEGSG
                     LGLAIAKTIVELHNGKFRVECEGDLFKAFVIIPKNKDDNNEE"
     gene            complement(1889..2587)
                     /locus_tag="K8M77_000725"
     CDS             complement(1889..2587)
                     /locus_tag="K8M77_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085240.1"
                     /translation="MEKEKFNVLVVDDEKEIRDAIDIYLRGEGINVIKSGDGFEALEI
                     LDKEDIHLVVLDIMMPKLDGMRTCLKIRESRNIPIILLSAKSEDSDKILGLNIGADDY
                     VTKPFNHLELVARVKSQLRRYDYPLNRENTQDLIVIKDLTIDTVNKQVSLRGENIKLT
                     ATEYKILTLLASHPGRIFSIKEIYERVWEEPFYKSENTVTVHIRRMREKIEINSKEPE
                     YIKVVWGLGYKIDK"
     gene            2841..3698
                     /locus_tag="K8M77_000726"
     CDS             2841..3698
                     /locus_tag="K8M77_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBI7085241.1"
                     /translation="MNYIKEKKELLIDNAFILIGCFISSLGVNLFLSNAKLLSGGATG
                     IALIFQYLMGVNSGIVVLLINIPLFILSYFKLSKQFTFNSAIGMLALSLSLMITAPVS
                     HLITLDDKLLYCVFGGAICGFGYGLVFSKGGSTGGTDIVTMVIRKKYSNFNIGSLSFI
                     LNMCIVGIGAIFFGLETALYTLISIFVQTVLVDKVIKGIHSKQLLLIITDKEQQVINY
                     IIEDLHRGVTSLLAEGEYTHDRKKMLYCLVTTRQMIELKNTIHYIDPNAFITIMDVSE
                     VKGKGFKNI"
     gene            complement(3959..4630)
                     /gene="cwlD"
                     /locus_tag="K8M77_000727"
     CDS             complement(3959..4630)
                     /gene="cwlD"
                     /locus_tag="K8M77_000727"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBI7085242.1"
                     /translation="MKKTIKIVAIIMLSFLVLNINLLKVNGEANNKVIVIDPGHGGID
                     GGAKSESGVIEKDINLSISLKTKAALESKGYKVIMTRSEDVGLYTEGKKVREKKIEDL
                     GNRVKIKKENKCDAFISIHQNMFPQKNCKGAQVWSANNEPSQKLGRIIQQKFKEEVDQ
                     NNKREAKVAKKEYKILNDGYEGASVIVECGFLSNPEECELLGKEDYQNKIANTLVNAI
                     DEYFK"
     gene            complement(4821..6434)
                     /locus_tag="K8M77_000728"
     CDS             complement(4821..6434)
                     /locus_tag="K8M77_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBI7085243.1"
                     /translation="MKDAIFTLMEVAGGLGLFLFGMKLMGEGLENAAGDKLKSILEKV
                     TKNPISAVLVGAFVTMVIQSSSATTVMVVGFVNAGLMNLAQAAGVIMGANVGTTITAQ
                     LVAFKLDEIAPLFVIVGVVLLMSAKQKKRKDIADIILGFGILFMGMGIMSSALKPLSH
                     SPMFSQLIMAIGDNWVLGIFTGLALTAILQSSSATTSILIALASTGSITINIVLPILF
                     GCNIGTCVTALISSIGANKTAHKAAAIHLMFNILGTLIFIPFLKPLGYIVQEMSPGDV
                     QRQIANAHTIFNITATIILVPLSKYMIIIVNKLIKGEDEVELFGPKYLDERLLETPVI
                     AAGQVQKETLRMANKAKENLELSMKAFRENNESLVKKVYDNEKLINILEEEITAYLVK
                     LSKCDLSAKESNLVASTFHIVTDIERIGDHVENIADLTLEKIGRKLKYSEDALKEIDY
                     IYDQTIKALDITIDAYANENVEEAGNIYEVERKIDASQKEFRENHIKRLSQGFCNAYD
                     GAIFMDLLSNFERIGDHATNIAESVMEVY"
     gene            complement(6811..7203)
                     /gene="rpsI"
                     /locus_tag="K8M77_000729"
     CDS             complement(6811..7203)
                     /gene="rpsI"
                     /locus_tag="K8M77_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBI7085244.1"
                     /translation="MAKVQYMGTGRRKKSVARVRLVPGEGRVIVNNREIETYFGLETL
                     RVVVNQPLVLTETKDKYDVLVNVHGGGLSGQAGAIRHGISRALLKADENLRPELKKAG
                     FLTRDPRMVERKKCGLKKARRSPQFSKR"
     gene            complement(7230..7664)
                     /gene="rplM"
                     /locus_tag="K8M77_000730"
     CDS             complement(7230..7664)
                     /gene="rplM"
                     /locus_tag="K8M77_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBI7085245.1"
                     /translation="MKSYIAKAQEVERKWYVVDAAGKPLGRVASQVASILRGKNKPTF
                     TPNVDCGDFVIVINAEKVVLTGKKLDQKMLRKHSLYAGGLKETPYREVLEKKPEFAFE
                     EAVRRMLPTGVLGRKMLKKLNVYRGAEHNHAAQKPEVLELRY"
     gene            complement(7811..8545)
                     /gene="truA"
                     /locus_tag="K8M77_000731"
     CDS             complement(7811..8545)
                     /gene="truA"
                     /locus_tag="K8M77_000731"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBI7085246.1"
                     /translation="MRNIKLTIEYDGTSYFGWQKQPIGNTIQQKVEEAIKKVTKEEVE
                     ILGSSRTDSGVHAKAYVANFKTNSNIPGKNFKAALNSKLPKDIVIMNSEEVAEDFHAR
                     YMTTGKTYCYTILNREEPPALERNYVYHVKKQLDVESMKEACKYFLGKHDFKAFQRPG
                     GTVKTSVRTITDIHIEIEGNKIKIYVSADGFLYNMVRLIVGTLLKVGRGKEKPEYIKE
                     VIDSGDRKKAGICVPPTGLCLEKVFY"
     gene            complement(8702..9508)
                     /locus_tag="K8M77_000732"
     CDS             complement(8702..9508)
                     /locus_tag="K8M77_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBI7085247.1"
                     /translation="MLKDITIGQYIPGDSFVHKLDPRTKILISFIFIASLFIVDKFWG
                     YIFIIAFLGATVLISKLQFKYLYKGLKPVFLLIAITAALNIFMIKGTEDTLIWHWKFL
                     YIYKEGIRTSIFMALRLILLIMGTSVLTLTTSPIELTDGIESLLKPFKKIGIPAHELA
                     MMMTIALRFIPTLIDETDKIMKAQKARGADFESGNIIQKAKSLIPLLIPLFISSFRRA
                     DELAMAMEARCYRGGDGRTRMKILKYSKNDFISFGMAGVLLVLSIVVRVF"
     gene            complement(9524..10381)
                     /locus_tag="K8M77_000733"
     CDS             complement(9524..10381)
                     /locus_tag="K8M77_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBI7085248.1"
                     /translation="MSIKIENLNHIYMPKTPFEKIALNNINCEIEDGEFVALIGHTGS
                     GKSTFIQHLNGLLNPTSGNIIVDGVNIADKKVKLSDIRKKVGLVFQYPEYQLFEETIE
                     KDIEYGPRNLGISEEEISKRVKKSMEMVGLDYETYKDKSPFDLSGGQKRRVAIAGVIA
                     MEPKVLILDEPTAGLDPKGREDILAQIRILHKEYGMTIIMVSHSMEDVAKIADRVIVM
                     NSGEIVLDGKIAEVFKEVEILEKIGLAVPQVTYLIRELRNKGFNISEEIFTISQAKEA
                     LLEIIRNNN"
     gene            complement(10366..11211)
                     /locus_tag="K8M77_000734"
     CDS             complement(10366..11211)
                     /locus_tag="K8M77_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBI7085249.1"
                     /translation="MGENMIKSEDLVFKYVNTEEQTEKVAINHVSMEVKKGEFLVILG
                     HNGSGKSTMAKHMNALLLPSGGKMYVDGLDTSDIENIWEVRRRAGMVFQNPDNQLVAT
                     IVEEDVAFGPENLGVDPKEIRERVDDSLKAVGMYEYRKHAPHLLSGGQKQRIAIAGIL
                     AMRPKCIVLDEPTAMLDPSGRNEVMKTIKEVNKKFGITIILITHYMDEAAQADRIIVM
                     DKGEKVMEGVPREIFSQVKKIKSIGLDVPQVTELAYELQKEGVDISTEILNIDEMVNA
                     LCQLK"
     gene            complement(11634..11975)
                     /gene="rplQ"
                     /locus_tag="K8M77_000735"
     CDS             complement(11634..11975)
                     /gene="rplQ"
                     /locus_tag="K8M77_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBI7085250.1"
                     /translation="MAGYRKLGRPTDQRKAMLRNLVTSFLKHGKIETTETRAKETRSL
                     AEKMITLAKRGDLHARRQVLAFVTEEEVVKNLFDNIAPKYAERNGGYTRMYKVGPRRG
                     DGAEVVILELV"
     gene            complement(11992..12939)
                     /locus_tag="K8M77_000736"
     CDS             complement(11992..12939)
                     /locus_tag="K8M77_000736"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBI7085251.1"
                     /translation="MLEIEKPVIQCVESNDNGTYGKFEIEPLERGYGITLGNALRRIL
                     LSSLPGVAPTSVKIDSVLHEFSTITGVKEDVTEIILNLKMLALTMEGEGPKTIYIDAQ
                     GPGVVTGADIKTDGDVEVVNKDLHIATLDNDGKLYMEIVVNRGRGYVTQNKNKTEDLP
                     LSAIAIDSIYTPVKRVNFSVQNTRVGQITDYDKLTLEIWTNGTIRIEEAISLSAKILI
                     EHFKLFMTLTDNANDVEIMIEKEEDKKEKALEMTIEELDLSVRSYNCLKRAGINTVQE
                     LAGKSMDDMMKVRNLGKKSLEEVERKLNELGLNLRLNDE"
     gene            complement(13024..13644)
                     /gene="rpsD"
                     /locus_tag="K8M77_000737"
     CDS             complement(13024..13644)
                     /gene="rpsD"
                     /locus_tag="K8M77_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBI7085252.1"
                     /translation="MARYTGATCKLCRREGMKLFLKGDRCYTDKCAFVRRSYAPGQHG
                     ASRKKLSNYGTQLREKQKAKRIYGVLEGQFRNTYERAEKMRGIAGENLLKLLEMRLDN
                     VVYRLGYGASRTEARQLVNHGHFLVNGKKVDIASFKVSVNDVITVCEKSRGSERFKMF
                     AENPKALPKWLEANVENFEGKVIAEPAREDIDVPVNETLIVELYSK"
     gene            complement(13668..14063)
                     /gene="rpsK"
                     /locus_tag="K8M77_000738"
     CDS             complement(13668..14063)
                     /gene="rpsK"
                     /locus_tag="K8M77_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBI7085253.1"
                     /translation="MAAQKVKKTRRRKERKNVEHGAAHIQSTFNNSIVTLTDAKGNAL
                     AWASAGGLGFKGSRKSTPFAAQMAAETAAKAAMEHGLKSVEVYVKGPGAGREAAIRSL
                     QAAGLEVTLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(14080..14445)
                     /gene="rpsM"
                     /locus_tag="K8M77_000739"
     CDS             complement(14080..14445)
                     /gene="rpsM"
                     /locus_tag="K8M77_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBI7085254.1"
                     /translation="MARIAGIDLPREKRVEIGLTYIYGIGLPTSQKILEVTGVNPDTR
                     VKDLTEEEVNSIRNYIKDLTVEGDLRREVALNIKRLVEIGSYRGIRHRKGLPLRGQKT
                     KTNARTRKGPKKTIANKKK"
     gene            complement(14682..14795)
                     /gene="rpmJ"
                     /locus_tag="K8M77_000740"
     CDS             complement(14682..14795)
                     /gene="rpmJ"
                     /locus_tag="K8M77_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBI7085255.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGRVMVICENPKHKQKQG"
     gene            complement(14808..15026)
                     /gene="infA"
                     /locus_tag="K8M77_000741"
     CDS             complement(14808..15026)
                     /gene="infA"
                     /locus_tag="K8M77_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBI7085256.1"
                     /translation="MSKSDIIEMQGTVLEALPNAMFEVELESGHKILAHISGKLRMNF
                     IRILPGDKVTVELSPYDLTRGRITWRAK"
     gene            complement(15034..15312)
                     /locus_tag="K8M77_000742"
     CDS             complement(15034..15312)
                     /locus_tag="K8M77_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HBI7085257.1"
                     /translation="MQETDLIGRLVTSNAGRDTGKSYIVIGLVDDHLLVANGATKTIE
                     MPKKKKIKHVVLTEILDDELKSCIISKDKNANLKIKRFLKLSSTNKEV"
     gene            complement(15401..16150)
                     /gene="map"
                     /locus_tag="K8M77_000743"
     CDS             complement(15401..16150)
                     /gene="map"
                     /locus_tag="K8M77_000743"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBI7085258.1"
                     /translation="MIILKNENEIDLMRKAGKILGETLNLLKEKVRPGITTTELDRIA
                     EEFITKHGATPSFKGLYGFPASLCISVNNQVIHGFPGSYELKDGDIVSIDCGVCLNGF
                     HSDAARTFGVGNISEEAEKLIRITEESFFKGIEKAYVDNRLTDISNEIQQYVEANGFS
                     VVRDFVGHGIGRKVHEDPEVPNFGRPGRGPKLMAGMVLAIEPMVNMGSYRVKTLDDGW
                     TVVTADGKLSAHYENTVAILPNGPEILTLIK"
     gene            complement(16150..16800)
                     /locus_tag="K8M77_000744"
     CDS             complement(16150..16800)
                     /locus_tag="K8M77_000744"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBI7085259.1"
                     /translation="MKIVLLGPPGAGKGTQAKSISNRYSIPHISTGDIFRKNISENTP
                     LGIEAKSYMDNGQLVPDEVTINMVKDRLQQDDCKNGYLLDGFPRTVHQAEALDNFLTE
                     REESIDTALLIEVPKEFILERMTGRRVCPSCGASYHIKFNPPTNDGKCDLCGSDVIQR
                     KDDTEETVKERLDVYENQTQPLIDFYKNKKQLSVVDGTQAINEVFESICKILGSDK"
     gene            complement(16827..18104)
                     /gene="secY"
                     /locus_tag="K8M77_000745"
     CDS             complement(16827..18104)
                     /gene="secY"
                     /locus_tag="K8M77_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBI7085260.1"
                     /translation="MLSTLRNAWKVQDLRKKIIWTVFLIAIFRMGSYIPVPGIDTDSL
                     KALTQSGSLVSFYDLISGGSFSRFSIFALGVVPYINASIIMQLLTVAIPKLEQLSKEG
                     DDGRKKIQKITRYASIVIGAITAYGSYVIIHNVGALKSNSPVSMFLILLTLVVGSTFL
                     MWLGDQITVKGVGNGTSLIIFANILSSLPMTGYQIYNLSKIGKINVVEVALFIFFTLA
                     LLAGVIYLSLAERRITVQYAGKAVGNKMMKGQSTHIPLSIIGTTVIAIIFAMSVMSFP
                     TTIAQFFPEAGWSQWITGSSYSPFNAKTWMYPVLYALLTIFFTWFYTQITFKPDEMAE
                     NMHKSSGFIPGIRPGKPTEIYLEKVLNRISMFGGCFAAIIAVVPILVANYTPFQGIQF
                     GGTSLLILVSVSLEIMRQLESQLTMRHYQGFLK"
     gene            complement(18107..18547)
                     /gene="rplO"
                     /locus_tag="K8M77_000746"
     CDS             complement(18107..18547)
                     /gene="rplO"
                     /locus_tag="K8M77_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBI7085261.1"
                     /translation="MKLHELRPAAGSKSAPKRVGRGTGSGLGRNAGKGEKGQNARSGG
                     GVRPGFEGGQMPLYRRLPKRGFTNPFTKHFVTINVDRLNIFDNGTEVTPELLLERRVV
                     SKLMDGVKILGNGNIEKSLTIAGCKLSKQAAEKIVAAGGKVEVK"
     gene            complement(18581..18760)
                     /gene="rpmD"
                     /locus_tag="K8M77_000747"
     CDS             complement(18581..18760)
                     /gene="rpmD"
                     /locus_tag="K8M77_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBI7085262.1"
                     /translation="MAMLKVTLVKSLIGRKKDHIATAHALGLRKIGKTVEHEGTPQIK
                     GMINKISYLLKVEEA"
     gene            complement(18766..19263)
                     /gene="rpsE"
                     /locus_tag="K8M77_000748"
     CDS             complement(18766..19263)
                     /gene="rpsE"
                     /locus_tag="K8M77_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBI7085263.1"
                     /translation="MRIDPSTLDLKEKVVSISRVTKVVKGGRNFRFSALVVVGDENGH
                     VGVGTGKSIEIPEAIRKGIEDAKKNLVEVSIVGTTVPHEIHGKFGTGDVLIMPATEGT
                     GVIAGGPARSVLELAGLKDVRAKSLGSNNPRNMVKATINGLANLRTAEDIAKLRGKSV
                     EEIIG"
     gene            complement(19284..19643)
                     /gene="rplR"
                     /locus_tag="K8M77_000749"
     CDS             complement(19284..19643)
                     /gene="rplR"
                     /locus_tag="K8M77_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBI7085264.1"
                     /translation="MFKKADRKEARERRHLRVRKKVFGTPERPRLSVYRSEKNIYAQI
                     IDDVNAVTLVAASSLDKAIEVKGSNKEAAKLVGELVAKRAIEKGINDVVFDRGGYVYH
                     GRVEALASGAREAGLKF"
     gene            complement(19674..20213)
                     /gene="rplF"
                     /locus_tag="K8M77_000750"
     CDS             complement(19674..20213)
                     /gene="rplF"
                     /locus_tag="K8M77_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBI7085265.1"
                     /translation="MSRVGKMPIAIPAGVTVTVTPENVVTVKGPKGELVKAMHKDINI
                     AVEDAQVVVTRPSDVKEHRALHGLTRALLNNMVVGVSQGFSKTLELNGVGYRAQLQGK
                     KLVMNLGYSHPVEVEAVDGVDFKLDGTTKVIVEGIDKEKVGAVAANIRSWRKPEPYKG
                     KGIKYSDEVIRRKEGKTGK"
     gene            complement(20310..20708)
                     /gene="rpsH"
                     /locus_tag="K8M77_000751"
     CDS             complement(20310..20708)
                     /gene="rpsH"
                     /locus_tag="K8M77_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBI7085266.1"
                     /translation="MVMTDPIADLLTRVRNANSVRHEVVEVPSSSVKKAIVNILLQEG
                     YLKGVEEYNDGVVPMMRLTLKYGANNERVITGLKRISKPGLRVYCKKDEVPRVLNGLG
                     IALISTSKGLVVDREARKLGLGGEVICYVW"
     gene            complement(20742..20927)
                     /locus_tag="K8M77_000752"
     CDS             complement(20742..20927)
                     /locus_tag="K8M77_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBI7085267.1"
                     /translation="MARKAMIEKWKKEPKYKTRAYTRCRLCGRPHSVLKKFGICRICF
                     RELAYKGEIPGCRKASW"
     gene            complement(20944..21483)
                     /gene="rplE"
                     /locus_tag="K8M77_000753"
     CDS             complement(20944..21483)
                     /gene="rplE"
                     /locus_tag="K8M77_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBI7085268.1"
                     /translation="MNRLQERYEKEVVPAMMEKFGYKNIMQVPKIEKVVINMGVGEAK
                     DNPKVLESAVSDLQIIAGQKPVLTRAKKSVANFKIRENMALGCKVTLRKTNMYEFVDK
                     LVSIALPRVRDFRGVSAKAFDGRGNYSLGIKEQLIFPEIEYDKVDKVRGMDIIFVTSA
                     NTDEEARELLRFLGMPFAQ"
     gene            complement(21506..21820)
                     /locus_tag="K8M77_000754"
     CDS             complement(21506..21820)
                     /locus_tag="K8M77_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBI7085269.1"
                     /translation="MKVHVRKNDTVVVISGKDKGKTGEVLRVIPKTGKVVVKGVNLVK
                     KHQKPNRQNMQGGIIEMEAAINSSKVMLFCEKCKKATRISHKLLEDGAKVRVCKKCGE
                     TF"
     gene            complement(21844..22212)
                     /gene="rplN"
                     /locus_tag="K8M77_000755"
     CDS             complement(21844..22212)
                     /gene="rplN"
                     /locus_tag="K8M77_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBI7085270.1"
                     /translation="MIQQQTLLKVADNSGAKEIMCIRVLGGSKRKFGNIGDVIVASVK
                     SATPGGVVKKGEVVKAVIVRSVRGLRRADGSYIKFDENAAVIIKDDKQPRGTRIFGPV
                     ARELRDNEFNKILSLAPEVL"
     gene            complement(22243..22497)
                     /gene="rpsQ"
                     /locus_tag="K8M77_000756"
     CDS             complement(22243..22497)
                     /gene="rpsQ"
                     /locus_tag="K8M77_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBI7085271.1"
                     /translation="MERNLRKKRLGRVVSDKMDKTIVVAVETKVRHPLYGKTVNRTTK
                     FKAHDENNEARFGDRVFIMETRPLSKDKRWRLVEIVEKAK"
     gene            complement(22519..22728)
                     /gene="rpmC"
                     /locus_tag="K8M77_000757"
     CDS             complement(22519..22728)
                     /gene="rpmC"
                     /locus_tag="K8M77_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBI7085272.1"
                     /translation="MKARELKELRTSNPQDLIKKLGDLKAELFNLRFQLATGQLENPM
                     RIREVKKSIAQIKTIIREEELKIEQ"
     gene            complement(22728..23162)
                     /gene="rplP"
                     /locus_tag="K8M77_000758"
     CDS             complement(22728..23162)
                     /gene="rplP"
                     /locus_tag="K8M77_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBI7085273.1"
                     /translation="MLMPKRVKHRKVQRGRMKGKATRGNFLAYGDFGIQATTCGWITS
                     NQIEAARIAINRYIRRGGKLWIKIFPDKPVTEKPAETRMGSGKGSVEYWVAVVKPGRV
                     LFELSGVDEEKAREAMRLASHKLPVKTKFVTRRDFEEMGGEE"
     gene            complement(23193..23861)
                     /gene="rpsC"
                     /locus_tag="K8M77_000759"
     CDS             complement(23193..23861)
                     /gene="rpsC"
                     /locus_tag="K8M77_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBI7085274.1"
                     /translation="MGQKVHPHGLRVGVIKDWDAKWYADKKNFADNLIEDQQIRKFIK
                     KELFSAGIAKIEIERSAKRVKLNIHTAKPGVIIGKGGSGIERLKASLKNIIAEKNVLI
                     NIVEVKNAETNAQLMAENIAAQLEKRISFRRAMKQTIQRAMKAGTLGVKTACSGRLGG
                     AEIARTEQYNEGTIPLQTIRADIDYGFAEADTTYGKIGVKVWVYNGEVLPTKKVEKKE
                     EANA"
     gene            complement(23882..24217)
                     /gene="rplV"
                     /locus_tag="K8M77_000760"
     CDS             complement(23882..24217)
                     /gene="rplV"
                     /locus_tag="K8M77_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009167817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBI7085275.1"
                     /translation="MEAKAIAKYVRMSPTKVGVVLDLIRGKNVNEAFAILKYTPRDAA
                     EVISKVLKSAVANAENNHELDANRLFVAEAHVGHGPTLKRFRPMDHGKAFRINKRTSN
                     ITLVVKERA"
     gene            complement(24245..24526)
                     /gene="rpsS"
                     /locus_tag="K8M77_000761"
     CDS             complement(24245..24526)
                     /gene="rpsS"
                     /locus_tag="K8M77_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBI7085276.1"
                     /translation="MSRSIKKGPFVHAGLLKKIEEMNQNGDKKVIKTWSRSSTIFPQM
                     IGHTIAVHDGRKHIPVYVTEDMVGHKLGEFVLTRTFKGHIKNEKTSKRK"
     gene            complement(24597..25430)
                     /gene="rplB"
                     /locus_tag="K8M77_000762"
     CDS             complement(24597..25430)
                     /gene="rplB"
                     /locus_tag="K8M77_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBI7085277.1"
                     /translation="MALKKFNPTTPSRRQMTMPTFEEVTTNAPEKSLLVSLKKTGGRN
                     AQGKITVRHHGGGAKRKYRIIDFKRNKDGIPAKVATVEYDPNRSAYIALVVYADGEKR
                     YILAPVGLKVGDTVVSGPEADIKPGNALQLKHMPVGTFVHNIELQAGKGGQMVRSAGT
                     SAQLMAKEGNYATLRLPSGEMRYVRIECKATVGTVSNTTHEIVNIGKAGRKRHMGWRP
                     TVRGSVMNPCDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKNKKYSDRFIIKRRNA
                     K"
     gene            complement(25485..25778)
                     /gene="rplW"
                     /locus_tag="K8M77_000763"
     CDS             complement(25485..25778)
                     /gene="rplW"
                     /locus_tag="K8M77_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBI7085278.1"
                     /translation="MKLTSHDIIRKPVITEKSMAAMAENKYTFIVHMAANKVQIKRAV
                     EEVFNVKVADVKTMRFEGKTKRVGVHIGKRADFKKAVITLAEGSSIEFFEGMQ"
     gene            complement(25778..26392)
                     /gene="rplD"
                     /locus_tag="K8M77_000764"
     CDS             complement(25778..26392)
                     /gene="rplD"
                     /locus_tag="K8M77_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBI7085279.1"
                     /translation="MPKVGLFNKEGQQVGDIQLNEQVFGVEVNKYALHQVVVAQLANK
                     RQGTQSAKTRSEVRGGGIKPWRQKGTGRARQGSIRAPQWIKGGVVFAPKPRDYRMSIP
                     KSMRKVAMTSALTSKVADMVVLEDLNFEAPKTKEAVKMLNAFEAKKTLIITAEVNENV
                     YKSARNIEGVTVMPVNNINVYDLLNCKTLMITKEAVNKIEEVYA"
     gene            complement(26417..27046)
                     /gene="rplC"
                     /locus_tag="K8M77_000765"
     CDS             complement(26417..27046)
                     /gene="rplC"
                     /locus_tag="K8M77_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017353789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBI7085280.1"
                     /translation="MKKAIIGKKVGMTQIFDENGRVIPVTVVEAGPCVVVQKKTVETD
                     GYDAIQVGFGELREKLVNKPRRGHFAKAGVSLRRTLKEFRMEDVANYNVGDEIKVDTF
                     EIGDKVDVSGVSKGKGFQGTIKRWNASRGPMSHGSKFHRAPGSMGAASDPSRTFKNKR
                     MPGHMGAKNTTVLNLEVVKIMPEKNIILIKGGIPGPNKGTIVIRNSVKA"
     gene            complement(27129..27437)
                     /gene="rpsJ"
                     /locus_tag="K8M77_000766"
     CDS             complement(27129..27437)
                     /gene="rpsJ"
                     /locus_tag="K8M77_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBI7085281.1"
                     /translation="MSKQKIRIRLKAFDHTILDQSAEKIVETAKSTGAKVVGPVPLPT
                     EKDVITILRAVHKYKDSREQFEVRTHKRLIDIVNPSPKTVDALMRLNLPAGVDIEIKL
                     "
BASE COUNT         7965 a         5504 c         3317 g        11028 t
ORIGIN      
        1 tatgttaatt aaaaaccttc ataaattttt acaactataa tttttttaaa tttattgaaa
       61 ttaagttaaa caacctctat cttattactc ttcattatta tcatctttat ttttaggaat
      121 tattacaaaa gctttaaata aatcaccttc acattcaacc ctaaactttc cgttgtgaag
      181 ttctacaata gttttagcta ttgctaagcc taatccagat ccttctattg ttgaatttct
      241 tgattcatct cctcttgcaa atctttctaa aatatccttc tcatcaaaat ctaaagcata
      301 ggaagaaata tttttaaatg atatttctaa ataattttct aaatccttta tttcaacata
      361 gaccctagtt ttttctaagc tatattttaa agcattagta attagatttt ggaataccct
      421 agacattctt tgaggatcta ttttaaccat gcattcctct gggccattta ttttaaattc
      481 aacttccttt tctgaatata catcttctac ctcagctata cattgatata caagttgaat
      541 tatatctata ttgtatttaa ataactctac ctttcctgaa gacatcttag aaacttcaaa
      601 caagtcatct ataagtttct ttaaagtttg agatttttta tctaatattt ctatatattc
      661 ttttctttcc tcttccttta tattatttaa ctgaagtata tttacataat ttattatgga
      721 tgttaatggt gtttttaaat catgtgaaac atttgatata agctcagttt ttaacttttc
      781 attctttata ccttcttcaa caagttcctt ataacctaat cttaaatcat taacatcatc
      841 tgctaattcc cttacttgtc tatcatcatt atcttttatc tctatatccg tatcaccttt
      901 tttaactttt tctattcctc ttattacatt atctaatcca tcatattttc ttataaactt
      961 cactataaaa tgaacaacta aaacaattgg aattatccat aggtatctat tgtctgttaa
     1021 aacatagtga tattgcttcc atacaaattc tatatttctc ataaatccat ttataagaat
     1081 atatattata gcaaatgaaa taacaaacat aacaacctta tcttttaaaa attcttctat
     1141 aaacatcttc tttgttatta aaacatttaa actatctaaa aatctaaaga aatacccatt
     1201 tttccaactc tctttatatg ctcttattcc catattttta atcataacta atttttttat
     1261 gattaaaagt aacattaaaa gagttgtagt catagttgct ataaaagcaa ttctaattga
     1321 atttatttca gagtcataaa gttctcctct tgagaaatat atttcttcat aattatctaa
     1381 agaatttatt tctttaaata ccacattatc atttttagat aaactttcta aaacttcttt
     1441 tggcgttgaa agattataag gtttttcata aaaccatact ctattagaat agtattctcc
     1501 tgttttctta tttataagaa ctacacctga ataactagta gctctttctt gaagctttgt
     1561 aataaatgtt tgtttttgct cctttgtctc taagctatct aatttgctta aatagttagt
     1621 ttcattataa agaaaatctg atatatctac agattgagct aataacctac actctttata
     1681 cttatgtgga tttattaatg catccctata aatgactcta ttaaatatat caggatttgc
     1741 cttagaattt aattttgcta tattatcaaa ggagtatcct ccaaataaga taatatataa
     1801 aacaggtata aacacagcaa atattactat aaacatattt tctatgcttc ttactgaaag
     1861 gctatagttt ttccaccatc ttgaaaaact acttgtcaat tttataccca agtccccaca
     1921 ccacctttat atactcaggt tcttttgaat ttatttctat cttttctctc attcttctta
     1981 tatgaactgt tactgtattt tcacttttat aaaaaggttc ttcccaaact ctttcataga
     2041 tttctttaat tgaaaatatt cttcctggat gtgatgctaa aagtgttaat atcttatact
     2101 ctgttgcagt taattttata ttttctcccc ttaaagaaac ctgcttattt acagtatcta
     2161 tagttaaatc ttttattact attaaatctt gagtattttc cctatttaaa ggataatcat
     2221 atcttcttaa ttgagatttt actcttgcaa caagttctaa gtgattaaaa ggtttcgtaa
     2281 cgtaatcatc tgctcctatg tttaaaccta atatcttatc actatcttct gattttgctg
     2341 aaagtaatat tattggtata tttctacttt ctcttatttt taaacaagtt ctcattccat
     2401 ctaacttagg catcattata tctaagacca ctagatgtat atcttcctta tctaaaattt
     2461 ctaaggcctc aaatccatct ccagacttta ttacatttat tccctctcct cttaaatata
     2521 tatctattgc atctcttatt tctttttcat catctacaac taaaacatta aacttttctt
     2581 tttccataat aaagcactcc ttaaacttat gcctccccta aatattagag gagaaaaatt
     2641 aaaattaacc tatttattta ttacgatttc attaagatat actttctact attaatttga
     2701 tgaaattctt agtatataca atgtatttta ccataaaaac acattagttg ctttttcaat
     2761 gtatttattg gctttacttt accaaaaaaa gcattataat tacctatgaa attatttatt
     2821 gtaaatttag gagaatccca atgaattaca tcaaagagaa aaaagaatta ctaattgata
     2881 atgcatttat tctaataggt tgctttattt catctttagg agtaaaccta tttttatcaa
     2941 atgcaaaact tcttagcggt ggagcaacag gtattgcact tatctttcaa tacttaatgg
     3001 gagttaactc aggtattgtt gttttactca taaacattcc attatttata cttagttatt
     3061 ttaaactatc aaaacagttt acatttaact cagctatagg aatgctagct ttatcacttt
     3121 cgttaatgat aacagcccca gtatcacacc ttattacctt agatgacaaa ttactatact
     3181 gtgtttttgg tggtgctatc tgtggttttg gttacggttt agtattttct aaaggtggat
     3241 caactggcgg aacagatata gttactatgg ttatacgtaa aaaatattca aattttaata
     3301 taggttcatt aagctttata ttaaatatgt gtatcgttgg tataggagca attttctttg
     3361 gactagaaac agcactttat actcttatat ctatatttgt acaaacagta ctagttgata
     3421 aagttattaa agggatacac tcaaaacaat tattgcttat aataaccgat aaagaacaac
     3481 aagttataaa ttatataata gaagatcttc atagaggagt aacttctctt ttagctgaag
     3541 gagaatatac tcatgacaga aagaaaatgc tttactgtct agttactaca agacaaatga
     3601 ttgaattaaa aaacacaata cattatatag atccaaatgc ctttataact ataatggacg
     3661 tttcagaagt taaaggaaaa ggctttaaaa atatataaaa tcaaaaaagc tatactcata
     3721 aggcatagct ttttttaatg tcataataac ttaatctact aaaatatata taataatgga
     3781 atgattttta ggagctttta tgaataagtt taatttagtg aatttcttaa tatgtttttt
     3841 atttattata ttatttttct taattctaaa ccttatattt tcatcttata aattttactc
     3901 aattactcca aatgatatgg aagttaaatt gacattacta atttttcttt aaataatctt
     3961 atttaaaata ttcatctata gcatttacta atgtatttgc aattttattt tgataatcct
     4021 ctttacctaa tagttcacat tcttctggat tagataaaaa accacattca actattacag
     4081 aagctccttc atatccatca tttaaaatct tatattcttt ttttgcaact tttgcttccc
     4141 tcttattatt ttgatcaact tcttctttaa atttttgttg tattattctg cctagttttt
     4201 gactaggttc attattagca ctccacactt gagcaccttt acaattcttt tgaggaaaca
     4261 tattttgatg tatgcttata aaagcatcac acttattttc ttttttaatt tttactctat
     4321 ttcctaagtc ttctattttc ttttctctaa ccttcttacc ctcagtatat aatcctacat
     4381 cctcacttct agtcattatg actttatatc ctttactctc taaagcagct tttgttttaa
     4441 gtgatattga taaattaatg tccttttcaa ttactccact ttcactcttc gctcccccat
     4501 ctatgcctcc atgacctggg tcaataacta ttaccttatt atttgcctcc ccattaacct
     4561 ttaataagtt aatatttaat actaaaaaag agagcattat aattgctact atttttattg
     4621 ttttcttcat taagaatacc tcctatttaa atctaaatta ttataatcct aaatcttaaa
     4681 aagaatatat ttactaataa atacttcaaa atattatctc ttttagaatt atgaaactca
     4741 atttattttt tcctatatat aaagaaatat tctaagtaaa agaaaaaaga atacctaata
     4801 ttaggtattc tttaatacta ttaataaact tccataacac tttcagctat gttagtagca
     4861 tgatcgccaa ttctttcaaa gttgcttaat aaatccataa atattgcacc atcataagca
     4921 ttgcaaaaac cttggcttaa tctcttaata tgattttctc taaactcttt ttgagaagca
     4981 tctatctttc tttctacttc atagatattt ccagcttcct ctacattttc atttgcataa
     5041 gcatcaattg ttatgtctag cgctttaatt gtttgatcat atatataatc tatttctttc
     5101 aaagcatctt cactatattt taattttctt cctatctttt ctaaagttaa atcagctata
     5161 ttctcaacat gatcaccaat tctctcaata tcagttacaa tgtgaaaagt tgatgctact
     5221 aaattacttt ctttagcaga taaatcacat ttagataatt taactaggta agcagttatt
     5281 tcctcttcca atatatttat aagtttttca ttgtcatata cttttttaac taagctttca
     5341 ttgttttctc taaaagcctt catacttaat tctaagtttt ctttagcttt attagccatt
     5401 cttaaagttt ctttttgaac ttgaccagca gctattactg gtgtttctaa taatctttca
     5461 tcaagatact taggtccaaa aagttcaact tcatcttcgc cttttattaa tttattaact
     5521 ataattatca tatacttact taatggtaca agtattatag tagcagttat attgaatatt
     5581 gtatgagcat tggctatttg tctttgaaca tctcctgggc tcatttcttg aacaatatat
     5641 cctaaaggtt ttaaaaatgg tataaatata agtgtaccta atatattgaa cattaaatgt
     5701 atggcagcag ctttgtgtgc tgttttattt gctccaatac ttgatattaa agctgttaca
     5761 catgttccaa tattacatcc aaataatatt ggcagaacta tatttatagt tatagaacca
     5821 gttgatgcta aagcaattaa tatactcgtt gtagctgatg aactttgtaa tatagccgta
     5881 agtgctaaac ctgtaaatat tcctaatacc cagttgtctc ctatagccat tataagttgt
     5941 gagaacattg gtgaatgaga taatggtttt aaagctgaac tcatgattcc cattcccatg
     6001 aataaaatac caaaacctaa tattatatca gcaatatctt ttctcttttt ttgttttgct
     6061 gacattaata aaacaacacc tactataaca aataatggtg ctatttcatc taatttaaag
     6121 gcaacaagtt gagcagtaat agttgtacct acattagccc ccatgattac tccagcagct
     6181 tgtgctaagt tcataagacc agcattaaca aaaccaacta ccattaccgt agtagcactt
     6241 gaactttgta ttaccattgt aacaaaggca ccaactaaaa ctgctgatat aggattttta
     6301 gtaactttct ctaatatact ttttaattta tcaccagcgg cattttctaa tccttctccc
     6361 attaacttca ttccaaaaag gaataaacct aatccaccag caacttccat taaggtaaaa
     6421 attgcatctt tcaagatact tctcctccac tttgcttaat ttgtaaaata caaacttatt
     6481 tttttaattt taaaatatca atcctataac attttacaat ataatatctc ttttgttaag
     6541 ttttaaagct tctttttaac ctaaacttaa tacagtatcc aattaataga aattttctac
     6601 aaataatatc caaataataa aaaaaactaa aataatactc ctctaatact taattatata
     6661 gccatttcta atacttttta atatttatta tcagaacata tatttgttta tactttgtta
     6721 ataattgaaa ttaaaatact ttatttaaaa tatataaata tataaaaaaa gaaggaggta
     6781 ttcctccttc tttctgataa aagcaatata ttatctcttt gagaattgtg gagatcttct
     6841 tgctttcttt aaaccacatt tctttctttc aaccattctt gggtctctag ttaagaatcc
     6901 agctttcttt aattctggtc ttaagttttc gtcagctttt aataaagctc ttgatatacc
     6961 atgtcttata gctcctgctt gacctgataa tccaccacca tgaacgttaa ctaaaacgtc
     7021 atatttgtct ttagtttcag ttaaaactaa tggttggtta actacaactc ttaaagtttc
     7081 taatccgaaa tatgtttcta tttctctatt atttactata actctacctt cacctggtac
     7141 aagtcttact cttgcaactg atttctttct tcttccagtt cccatgtatt gaacttttgc
     7201 catttgaact cctcctcccg agcttataat tagtatctta actcaagtac ttctggcttt
     7261 tgagcagcgt gattgtgctc agctcctctg tacacgttta atttctttaa catttttctt
     7321 cctaaaacgc ctgttggaag cattcttcta actgcttctt cgaaagcaaa ttcaggtttc
     7381 ttttctaaaa cttctctgta tggagtttct tttaatccac cagcgtataa gctgtgtttt
     7441 cttaacattt tttggtctaa tttcttacca gttaaaacaa ctttctcagc gtttataacg
     7501 ataacgaaat ctccacagtc aacatttggt gtaaatgttg gcttgttctt tcctcttaat
     7561 attgaagcaa cttggctagc aactcttcct agtggctttc cagcagcgtc aacaacatac
     7621 cattttcttt caacttcttg tgcttttgca atgtatgatt tcatcgtttt ccctccctag
     7681 aattttaaaa taggttttat atgtcctcgt actttatgtt aagattcata ttgaatcccg
     7741 tacatctctc ttacaaacaa atacaattat aatacacgct tacgggcgtg tcaatacatt
     7801 tcatctaatt ttagtaaaaa actttttcta agcataatcc tgttggtgga acacaaattc
     7861 cagctttttt tctatcacca gaatctatca cttcctttat atattctggc ttctcttttc
     7921 ctcttcccac tttaagtaat gttcctacaa ttaatcttac catattatat aaaaaaccat
     7981 ctgccgacac atatatttta attttatttc cttctatttc aatgtgaata tcggttattg
     8041 ttcttacaga ggtcttaact gtcccacctg gtctttgaaa agccttaaag tcatgttttc
     8101 ctaaaaagta tttacatgct tctttcatag attcaacatc taattgcttt ttaacatgat
     8161 atacataatt tctttctaat gctggtggtt cttctctatt taatattgtg taacaataag
     8221 ttttacctgt agtcatatac ctagcatgaa aatcctcagc tacctcttct gaattcatta
     8281 ttactatatc ctttggtaac tttgaattta atgcagcctt aaaatttttc cctggtatat
     8341 tagaattagt tttaaaatta gccacatagg ccttagcatg gactcctgaa tccgttctac
     8401 tacttcctaa tatttctacc tcttctttag taaccttctt tatagcctct tctaccttct
     8461 gctgaatagt atttcctata ggctgttttt gccacccaaa ataagatgtt ccatcatatt
     8521 ctatagttaa ttttatattc ctcatataat cattccttta ttttttataa aacaatattt
     8581 taaatcataa ataattaatt tttcaaacaa agaaagacta caaatttgca gcctttcttt
     8641 tatattatca atatttattt tatattaaaa gtctaaatgt aaaaacaaaa tttaatttat
     8701 actagaaaac tcttactact attgataaaa ccaaaagtac tccagccatt ccaaaagaaa
     8761 taaaatcatt tttagaatac tttaatatct tcattctagt tcttccatcg ccacctctat
     8821 agcatcttgc ttccatagcc atagctaact catcagctct tctaaaagaa ctaataaata
     8881 aaggaattaa tagtggtatt aaactcttag ctttttgtat tatgtttcct gattcaaaat
     8941 ctgcacctct agctttttgt gctttcatta ttttatcagt ttcatcaatt aaagttggta
     9001 taaatcttaa agcaattgtc atcatcattg ctaattcatg agctggaatc cctatctttt
     9061 taaatggttt caataaactt tcaataccat cagttaattc tattggtgat gttgtaagtg
     9121 ttaatactga agttcccatt ataagcaaga taagtcttaa tgccataaat atagatgttc
     9181 ttattccttc cttataaata taaagaaact tccaatgcca tataagagta tcttccgtac
     9241 cttttatcat aaaaatatta agcgctgctg ttatagctat taataaaaat acaggtttaa
     9301 gtcctttata taaatattta aattgtaatt tagatattaa tactgttgca cctaaaaagg
     9361 caattatgaa tatatacccc caaaacttat ctactataaa taatgaagca ataaaaataa
     9421 atgatattaa tatcttagtc cttggatcta atttatgtac aaaagaatcc ccaggaatat
     9481 attgacctat agtaatgtcc tttaacataa tttcttatcc ttcctaatta ttattcctta
     9541 ttatttctaa aagagcttcc ttagcttgag aaattgtaaa tatctcttct gatatattaa
     9601 agcccttatt tcttaactct cttattagat aagttacttg aggaactgcc aatcctattt
     9661 tttctaaaat ttcaacctct ttaaatacct ctgcaatctt tccatctaaa actatttctc
     9721 cactattcat aacaattact ctatcagcaa tcttagctac gtcttccatg ctatgactaa
     9781 ccattattat agtcattcca tactctttat gaagaattct tatttgagca agtatgtcct
     9841 ctcttccctt tggatctaaa ccagctgttg gttcatctaa tataagaacc ttaggttcca
     9901 tagctataac cccagctata gcaactcttc tcttttgacc tccacttaga tcaaaaggag
     9961 atttgtcttt atatgtttca taatcaagcc caaccatctc catagatttt ttaactcttt
    10021 tacttatttc ttcttcacta attcctaagt ttcttggacc atactcaata tctttttcaa
    10081 tagtctcttc aaataattga tactctggat attgaaatac taatcctact ttttttctaa
    10141 tatcgcttaa tttaactttt ttatctgcta tattaacccc atcaactatt atatttccac
    10201 tcgtaggatt taataatcca tttaaatgct gtataaatgt tgattttcct gaaccagtat
    10261 gcccaattaa tgctacaaac tctccatctt ctatttcaca gtttatatta tttagtgcta
    10321 ttttttcaaa aggtgtttta ggcatatata tgtgatttaa attttctatt ttaattgaca
    10381 taaagcattc accatctcat ctatatttaa tatttctgtt gatatatcta ctccctcttt
    10441 ttgaagctca taagctaatt cagtaacttg aggaacatct agaccaatcg actttatttt
    10501 tttcacttga gaaaaaattt ccctaggaac tccttccatt accttttccc ctttatccat
    10561 aactattatt ctatcagctt gtgcagcttc atccatatag tgagttataa gtattatagt
    10621 aattccaaac tttttattta cttcctttat agttttcatt acttcatttc tacctgaagg
    10681 atctagcatt gctgtaggtt catctaatac tatgcattta ggtctcattg ctaatatacc
    10741 agcaatagca attctctgct tctgacctcc tgataataaa tgtggtgcat gttttctata
    10801 ctcatacata cctactgcct ttaatgaatc atctactctt tctcttatct cctttggatc
    10861 aacacctaaa ttctctggtc caaaagctac atcctcttca actattgttg ctactaattg
    10921 attatctgga ttttgaaaaa ccattccagc tcttcttcta acttcccaaa tattttctat
    10981 atcagaagta tctaatccat ctacatacat ctttccacca ctaggaagta ataaagcatt
    11041 catatgcttt gccatagttg attttcctga accgttatga cctaatataa ctaaaaattc
    11101 tcccttttta acttccatag aaacgtgatt tattgcaacc ttttcagttt gttcttcagt
    11161 attgacatat ttaaaaacta agtcttcact tttaatcata ttttctccca tactagcctc
    11221 ctgtaaagct acctttaact actttagagt taaaatacat aactcttttt ctcaatatgt
    11281 attgagttta ataatttttt tatgtataaa aaaaatacct acttataaaa taatactatt
    11341 aataatttta ttttgtaaat agatatttcc ttattttaac cttttatata aaattatctt
    11401 ccttaacata tgtttattaa aaaatttctt taaacacata atataccttt aaattcaaaa
    11461 ctattatagg ttaaatctaa aattttatac tagtagtaaa aataatatat tctgttttat
    11521 tatatattac tttgttatat atatttaaaa tactcatgat aaaaaataat ataaaatatt
    11581 ttatatgaca aaagaggatt aagtaaaaac ttaatcctct ctaaagaaat atattatact
    11641 aattcaagta taacaacttc agctccgtct cctcttctag gaccaacttt gtacattcta
    11701 gtgtatccac cgtttctttc agcgtacttt ggagctatgt tgtcaaataa gtttttaaca
    11761 acttcttctt ctgtaacaaa tgctaacact tgtcttcttg cgtgtaaatc tcctcttttt
    11821 gcaagagtga tcattttttc tgcaaggctt ctagtttcct tagctcttgt ctcagtagtt
    11881 tctattttac catgctttaa gaagcttgta acaagatttc taagcatagc ttttctttga
    11941 tcagtaggac gacctaactt acgatatcct gccatggctt tacctcctca cctactcgtc
    12001 atttagtctt aaatttaagc ctaactcgtt taactttctt tctacttctt ctagagattt
    12061 cttacctaag tttctaactt tcatcatatc atccatgctc ttgcccgcaa gctcttgaac
    12121 agtgtttata cctgctctct ttaagcaatt gtatgatctt actgataagt caagctcttc
    12181 tatagtcatc tctaaagctt tttctttttt atcttcttct ttttcaatca ttatctcaac
    12241 atcattagcg ttgtcagtta aagtcatgaa taatttgaag tgctctataa gaattttagc
    12301 tgataatgaa atcgcttctt ctattcttat agttccatta gtccaaattt ctaatgtaag
    12361 tttatcatag tcagtaattt gaccaactct tgtattttga acagagaaat taactctctt
    12421 tacaggagtg tatatagaat ctatagcaat agctgataat ggaaggtctt ctgttttatt
    12481 tttgttttga gtaacatatc ctcttcctct attaactacg atttccatat ataacttacc
    12541 atcgttgtct aatgtagcga tatgtagatc tttgtttaca acttctacat caccatctgt
    12601 ttttatatct gctccagtaa caacacctgg tccttgagca tcaatataaa tagtttttgg
    12661 tccttcacct tccatagtaa gtgctaacat tttaagattt aagattatct ctgtaacatc
    12721 ttctttaaca cctgttattg ttgaaaactc atgaagaact gaatctattt taactgaagt
    12781 tggagccact ccaggtaaag aagaaagtaa tattcttctt aatgcattac caagagttat
    12841 tccataacct ctttctaatg gttctatctc aaacttacca tatgttccgt tatcgtttga
    12901 ttcaacacat tgaattactg gcttttctat ttctaacatt taaaaaccct ccttaatatt
    12961 aaaatggcaa ccttatgagg gcaactgatc caattgtaaa agtactctta agatcaataa
    13021 ttattatttg ctgtataact cgacgattag agtctcgtta actggtacgt cgatatcctc
    13081 tcttgcaggc tcagcaataa ctttaccttc gaagttttca acgttagctt ctaaccattt
    13141 tggtaaagct tttggatttt cagcaaacat tttgaatctt tctgatcctc tgctcttttc
    13201 acaaactgtt ataacatcgt taactgatac tttgaatgat gctatatcaa cttttttacc
    13261 atttactaag aaatgaccat ggtttactaa ttgtctagct tctgttcttg aagcaccata
    13321 tcctaatctg taaacaacgt tatctaatct catttctaat aatttaagta agttttcacc
    13381 ggcaattcct ctcatttttt cagctctttc atatgtgttt ctgaactgac cttcaagaac
    13441 tccgtatatt ctcttagctt tttgcttctc tcttaattgt gttccgtagt tagataattt
    13501 ctttctgctt gctccgtgtt gtcctggtgc ataacttctt ctaacgaaag cacatttatc
    13561 tgtataacat ctatcccctt ttaggaataa tttcatacct tctcttctac ataacttaca
    13621 tgtagctcca gtatatcttg ccattcatta cacctcccgt gacgttacta aactcttctt
    13681 ctttttggtg gtctacatcc attgtgtggg attggagtta catcttttat taaagtaact
    13741 tctaatccag ctgcttgtaa gcttcttata gctgcttctc ttccagcacc tggtcctttt
    13801 acgtaaacct caacgctttt taatccgtgt tccatagctg ctttagctgc tgtttcagct
    13861 gccatttgag ctgcaaatgg tgtgctcttt cttgaacctt tgaatccaag gccaccagca
    13921 cttgcccatg ctaatgcatt tccttttgca tcagttaaag taactattga gttattgaat
    13981 gttgattgga tgtgtgctgc accatgctca acattttttc tttcttttct tcttctagtt
    14041 tttttaactt tttgagctgc cattgtcttc cctccttaac tatttcttct tattagctat
    14101 tgtcttctta ggaccttttc tagttctagc attagtctta gttttttgtc ctctaagtgg
    14161 aagacctttt ctatgtctga ttcctctata tgaaccaatc tcaactaatc tctttatgtt
    14221 taaagcaacc tctcttctta agtcaccttc aactgttaaa tctttaatgt agtttcttat
    14281 tgagtttact tcttcctcag taagatcctt aactcttgta tcaggattaa ctccagttac
    14341 ttcaagaatt ttttgtgatg taggtaatcc tataccatat atataagtta gacctatttc
    14401 aactcttttt tctcttggta ggtcgatacc tgctattctt gccattaatg ttacacctcc
    14461 tggtaattaa aatacgaaat tgatttacat taaataagta actcaattgc tctattgttt
    14521 atttatattt aaaattttag gttgatggtt ttacccatca cagccgcacc taaaatttat
    14581 ttgctattat ttaaatgcct atataatcat attttacttt taaataaaag tcaataagaa
    14641 ttcgctccta attgaccttt ttattatgtt ttatttacct attagccttg tttttgtttg
    14701 tgcttaggat tttcacagat taccattact cttccttttc tttttataac tttgcacttt
    14761 tcgcaaatag gcttaactga tggtcttact ttcatcgcta accctcctta ttttgctctc
    14821 catgtaattc tacctcttgt aagatcatat ggtgaaagtt ccacagtaac cttatctcct
    14881 ggtaaaattc ttatgaagtt cattcttaac tttcctgaga tatgtgctaa tattttatgc
    14941 ccactctcta attcaacttc aaacattgca tttggtaaag cttctaaaac tgtaccttgc
    15001 atttcaatta tatcactttt tgacataagg taatcaaacc tccttgttag tgctgctaag
    15061 ctttaaaaat ctttttattt taagatttgc attcttatcc tttgatatta tacatgattt
    15121 taactcatca tccaatattt cagtaagcac tacatgtttt atttttttct tttttggcat
    15181 ttcaatagtc ttagttgctc cattcgccac tagtagatga tcatctacta atccaatcac
    15241 aatatatgat tttcctgtat ctcttccagc atttgaagta actaatctac cgattaaatc
    15301 agtttcttgc aacttatcca cctcaaagat taccttagtc ataaactaca aattccttat
    15361 aggcaaatgt taatttataa ctaagcaata tatattaaaa ctattttata agtgttaata
    15421 tttcaggacc attaggtaaa attgcaactg tgttttcata gtgtgcagaa agtttcccat
    15481 ctgctgttac tactgtccag ccatcatcta aagtcttgac tctatatgat cccatgttta
    15541 ccatcggttc aatagctaat accattcctg ccattaactt tggacctctg cctggtctac
    15601 cgaaattagg aacttcagga tcttcatgga cttttcggcc aattccatgt cctacaaagt
    15661 ctctaacaac agaaaatcca ttggcttcaa catattgctg tatttcatta gaaatatccg
    15721 ttaatctatt gtctacatat gccttctcta tccccttaaa aaaggattct tcagtaattc
    15781 taataagctt ttctgcctct tctgatatat tacctacacc aaaagttcta gcagcatcac
    15841 tgtgaaatcc atttagacaa actccacaat caatagaaac aatgtctcca tcctttaatt
    15901 cataacttcc tgggaaacca tgaattactt gattattaac agatatacat aatgaagctg
    15961 ggaaaccata caatcctttg aaggacggtg tagctccatg cttagttata aactcttcag
    16021 ctattctatc taattccgta gtagtaatac caggtcttac tttctcctta agcaggttaa
    16081 gtgtttctcc aagaatcttt cctgctttcc tcattaggtc gatttcattc tcattcttaa
    16141 gaattatcat tacttgtcgc tccctaatat tttacagata ctctcaaaga cctcgtttat
    16201 tgcttgtgtt ccatctacta ctgaaagttg ctttttgttt ttataaaaat caattaatgg
    16261 ttgtgtttga ttttcataca catcaagtct ttccttaact gtttcttcag tgtcatcttt
    16321 tctttgaata acatctgatc cgcaaagatc acattttcca tcattagttg gtggattaaa
    16381 ctttatgtga taacttgctc cacatgaagg acaaactctt cttcctgtca ttctttcaag
    16441 tatgaattct ttaggtactt ctattaaaag agctgtgtct attgactctt ctctttcagt
    16501 taagaagtta tctaaagctt ctgcttgatg aactgttctt gggaaaccat ctaataaata
    16561 tccgttctta caatcatctt gttgaagtct atcttttacc atgttgatag ttacttcatc
    16621 tggaactaac tgtccattat ccatgtatga tttagcctca atacctaaag gagtgttctc
    16681 tgaaatattt tttctaaaga tatctcctgt agatatatga ggaattgaat atctattgct
    16741 aattgatttt gcctgtgttc cttttcctgc tccaggagga cctaataaaa ctatcttcac
    16801 gggcatcatc tccataactt gtatttctat tttaagaatc cttggtaatg tctcattgtt
    16861 aattgagatt ctaattgtct cattatttct aatgaaacag aaactagaat taacagtgat
    16921 gttcctccaa actgtattcc ttggaaagga gtatagtttg caactaaaat aggaactact
    16981 gctataatag cagcaaaaca tccaccaaac attgaaattc tatttaatac tttctctaaa
    17041 taaatctctg ttggttttcc aggtcttatt ccaggaatga aacctgatga tttatgcata
    17101 ttctcagcca tttcatctgg cttaaatgtt atttgagtat agaaccaagt aaagaatata
    17161 gtcagtaatg catataatac tggatacatc caagtctttg cattgaaagg actgtaagaa
    17221 gatcctgtaa tccattgtga ccatccagct tctgggaaga attgagctat agttgttggg
    17281 aaactcataa cagacatagc aaagattatt gctataacag ttgttccaat aatacttaat
    17341 ggaatatgag ttgattgtcc cttcatcatt ttattaccta cggctttacc tgcatattgt
    17401 acagtaattc ttctttcagc taatgataag taaattacac ctgctaataa agctaatgta
    17461 aagaaaataa ataatgctac ttcaacaaca tttattttac ctatcttact taagttatat
    17521 atttgataac cagtcattgg taaacttgat aatatatttg caaatattat caaggaagta
    17581 ccatttccta ctcctttaac tgtgatttga tctcctaacc acattaagaa agttgatcca
    17641 actactagtg ttagtaatat taaaaacata ctaactggag aattgctctt aagtgcgcca
    17701 acattgtgaa ttatcacata acttccataa gcagtgattg ctccaataac aattgaagca
    17761 tatctagtta tcttttggat tttctttctt ccatcatctc cctctttaga caattgttct
    17821 aatttaggaa ttgcaactgt taaaagttgc ataatgattg aagcattgat gtatggtaca
    17881 acacctaaag cgaatatact aaatctacta aaagaaccac cagaaattaa atcatagaag
    17941 cttactaaac ttcctgattg tgttaaagct tttagactat ctgtatcaat tccaggaaca
    18001 ggaatataac ttcccatcct gaaaattgct attaagaata cagtccaaat tatttttttt
    18061 cttaaatctt gaaccttcca ggcattacgt agggttgata gcacgattat ttcacctcaa
    18121 cttttcctcc agctgcaact attttttcag ctgcttgctt tgataacttg caacctgcga
    18181 tagttaagct cttttctatg tttccatttc ctaagatctt aactccgtcc attagtttac
    18241 ttactactct tctttctaat agtaattctg gagtaacttc tgttccatta tcgaatatgt
    18301 ttaatctatc aacattgatt gttacaaaat gttttgtaaa tggattagtg aatcctcttt
    18361 taggaagtct tctgtataat ggcatttgac caccttcgaa tccaggtcta actccaccac
    18421 ctgatctagc attttgaccc ttttcacctt ttccggcatt tcttcctaag cctgaaccag
    18481 tacctctacc aactcttttt ggagctgatt tactaccagc tgctggtctt aattcatgaa
    18541 gtttcatatg ttattacacc tcctctaacg ctctatttaa ttatgcttct tcaactttta
    18601 gtagataact tattttgttt atcatacctt ttatttgagg agttccctca tgctctactg
    18661 ttttacctat ttttctaagt ccaagggcat gcgcagtagc gatatggtct ttctttctac
    18721 caattaagct tttaactaat gttaccttta gcatcgctat ccctcctaac ctattatctc
    18781 ttcaacagat tttcctctta atttagctat atcttcagct gttcttaagt tagctaatcc
    18841 gttgatagtt gcttttacca tgtttcttgg gttgtttgaa cctaatgatt tagctctaac
    18901 atcctttaat cctgctaatt caagaactga tcttgcagga cctccagcta taactcctgt
    18961 accttcagta gctggcatta taagtacatc tcctgtaccg aattttccgt gtatttcatg
    19021 aggaacagta gttccaacta ttgaaacttc tactaagttt ttcttagcat cttctattcc
    19081 ttttcttatt gcttcaggga tttcgataga tttaccagtt ccaacaccaa cgtgtccgtt
    19141 ctcgtctcca acaacaacta acgcactgaa tctgaagttt ctaccaccct taacaacctt
    19201 agtaactctg cttatactaa caaccttttc tttaaggtct agtgtgctag gatcgattct
    19261 catgtatttc ccctccttgt agattagaat tttaatccgg cttctcttgc tccgctagct
    19321 aaagcctcaa ctcttccgtg atatacgtat ccacctctgt cgaatactac atcatttatt
    19381 cctttttcta tagctctttt agcaactaat tctccaacta atttagcagc ttctttgttg
    19441 cttcctttaa cttcgatagc tttatctaag cttgaagcag ctactaaagt aacagcatta
    19501 acgtcgtcta ttatttgagc atatatgttc ttttcacttc tatatacaga taatcttggt
    19561 ctttctggag ttccgaatac cttcttacga actcttaggt gacgtctttc tcttgcttct
    19621 tttctgtctg cctttttgaa cataaggttc actcctttct atattagtat ttattattta
    19681 ccagttttac cttcttttct tcttataact tcatcagagt acttgattcc tttaccttta
    19741 tatggttcag gttttctcca tgatctgatg ttagcagcaa ctgctccaac tttttcttta
    19801 tcgatacctt caacgataac tttagttgtt ccgtctaatt tgaagtctac tccatcaact
    19861 gcttcaactt ctactggatg tgaatatcct aagttcataa caagtttctt tccttgtaat
    19921 tgagctctgt atccaactcc gtttaactct aaagtttttg aaaaaccttg gcttactcca
    19981 acaaccatgt tgttaagtaa agctctagtt aatccgtgaa gtgctctgtg ttctttaacg
    20041 tcacttggtc tagtaacaac tacttgagca tcttctacag ctatattaat gtctttgtgc
    20101 atagctttaa ctaactcacc cttagggcct tttactgtaa caacgttctc tggtgttaca
    20161 gtaacagtta ctccagcagg gatagctata ggcattttac ctactcttga cataatgcgc
    20221 acctcctgtg aatttggtcc ttatattttt ttaaatcatc tctttgcttc aatctattta
    20281 aatctatgtt attttctcac tctaaaaaat taccaaacgt agcagataac ttctccgcct
    20341 aatcctaatt ttctagcttc tctgtcaact actaatcctt ttgaagttga tattaaagcg
    20401 atacctaatc cgtttaatac tctaggaact tcatctttct tgcagtaaac tcttaagcct
    20461 ggttttgata ttctctttaa accagttata actctttcat tgtttgctcc gtactttaaa
    20521 gttaatctca tcataggaac tacaccatcg ttgtactcct ctactccttt aagataacct
    20581 tcttgtaata atatatttac gatagccttc ttaacacttg aagaaggtac ttcaacaact
    20641 tcgtgtctta ctgagttcgc gtttctcaca cgagttagta agtctgcgat aggatctgtc
    20701 ataaccatta tattgtgcct cctttcattc tactagttat attaccagct tgcctttcta
    20761 catccaggaa tttcaccttt gtaagctaat tctctgaagc agatacggca aattccgaat
    20821 ttctttaata cagaatgtgg tcttccacat aatctacatc tagtatacgc tctagttttg
    20881 tatttaggtt cttttttcca tttttcgatc attgccttac gagccatgcg tatcccctcc
    20941 ttattattga gcgaatggca ttccaaggaa tcttaataat tctcttgctt cttcgtctgt
    21001 gttagcacta gtaacgaaga ttatatccat tcctcttact ttatctactt tatcatactc
    21061 gatttctggg aatattaatt gttccttaat tcctaatgag tagtttcctc ttccatcaaa
    21121 tgctttagct gaaactcctc tgaagtctct tactcttggt aaagctattg aaactaattt
    21181 atctacgaat tcgtacatat tagtctttct taaagtaact ttacatccta aagccatgtt
    21241 ctctcttatt ttaaagttag caactgactt tttagctctt gttaatacag gcttttgtcc
    21301 agcgattatt tgtaaatcac taacagctga ttctagaacc ttaggattgt ctttagcttc
    21361 tcctactccc atgtttataa caactttttc tattttagga acttgcatta tgtttttgta
    21421 tccgaacttt tccatcatag ctggaactac ttctttttca tatctttctt gaagtctatt
    21481 cacgtaaagt acctcctttc aaattttaga atgtttctcc acacttctta catactctta
    21541 cttttgcacc gtcttctaat aacttatggc ttattcttgt agcctttttg catttttcac
    21601 agaataacat aacttttgag ctgtttattg cagcttccat ttctattatt ccaccttgca
    21661 tgttttgtct attaggtttt tgatgctttt taactaagtt aactccttta actactactt
    21721 taccagtctt aggaattact cttaaaactt caccagtttt tcctttatct ttacctgata
    21781 taactactac tgtgtcgttc tttctaacat gtaccttcat cttagccacc tcctaatcgt
    21841 ttattataga acttctggtg ctaatgataa gattttattg aactcattat ctcttagctc
    21901 tcttgcaact ggtccaaaga tacgagttcc tcttggttgc ttatcatctt ttattattac
    21961 agctgcattt tcatcaaatt tgatgtatga accgtcagct cttcttagtc ctcttactga
    22021 tcttacaata actgctttaa ctacttcgcc ttttttaaca actccgcctg gtgttgcact
    22081 tttaacgcta gcaactatta catctccgat atttccgaat tttcttttag atccgcctaa
    22141 aactcttatg cacattattt cttttgcacc tgagttgtca gcaaccttta gtaaggtttg
    22201 ttgttgtatc atgaaattaa cctcctttca gactttagaa aactattttg ctttctctac
    22261 tatctcaaca agtctccatc tcttatcttt tgataatggt ctagtttcca ttataaaaac
    22321 tctatctccg aatctagcct cattattttc atcatgagct ttgaacttag ttgttctgtt
    22381 aacagttttt ccgtatagtg gatgtctaac ctttgtttca accgcaacaa cgatagtctt
    22441 atccatttta tcagaaacaa ctctacctaa tctctttttt cttaaatttc tttccacagc
    22501 ggttacctcc tttcaggatt actgctcaat ctttaactct tcttctctta tgattgtttt
    22561 gatttgagct attgattttt ttacttctct gattctcatt gggttttcta attgacctgt
    22621 agctaattgg aatcttaaat tgaataattc agctttaagg tctcctaatt tctttattaa
    22681 atcttgagga ttgcttgttc ttagttcttt taactctcta gctttcatta ttcttcacca
    22741 cccatttcct caaaatctct tcttgtaacg aacttagtct ttacaggaag tttgtgtgaa
    22801 gcaagtctca tagcttctct tgctttctct tcatcaactc cagataactc gaataaaact
    22861 ctacctggtt taactactgc tacccagtat tcaactgaac ctttacctga acccattctt
    22921 gtttcagctg gtttctcagt tacaggtttg tctgggaata tttttatcca aagctttccg
    22981 cctcttctta tatatctatt gatagctatt ctcgcagctt ctatttggtt actagtgatc
    23041 catccacaag ttgtagcttg gattccgaaa tctccgtaag ctagaaagtt tcctctagtt
    23101 gccttacctt tcattctacc acgttgtacc ttacgatgtt taactctttt aggcattaac
    23161 atgttatatt cctcctttct tagtgcatcc tattatgcgt tagcttcttc ctttttttca
    23221 acttttttag ttggaagaac ttctccattg taaacccaca ctttaacacc gattttaccg
    23281 taagttgtat cagcttcagc aaatccatag tcgatgtcag ctcttattgt ttgtagtggg
    23341 attgttcctt cattgtattg ctcagttctt gctatttcag ctccacctaa tcttcctgaa
    23401 caagcagttt taacacctaa tgtgccagct ttcattgctc tttggatagt ttgcttcata
    23461 gctcttctga atgatattct cttttctaat tgagcagcaa tgttttcagc cattaattga
    23521 gcgttagttt cagcgttctt aacctcaaca atgtttatta aaacattttt ctcagctatt
    23581 atatttttta agctagcttt taatctttcg attcctgaac caccttttcc gatgattaca
    23641 cctggcttag ctgtgtgtat gtttaactta actcttttag ctgatctttc gatttcaatc
    23701 ttagcgattc cagctgagaa aagttctttc tttatgaatt ttcttatttg ttggtcttcg
    23761 attaagttat cagcaaaatt tttcttatca gcataccatt ttgcatccca atctttaatt
    23821 acaccaactc ttagtccgtg aggatgtact ttttgtccca tgcttttccc tccttacctt
    23881 attaagctct ttctttaaca acaagtgtta tgttacttgt tctcttgttt attctgaatg
    23941 ctttaccatg atccattggt ctgaatcttt ttaatgttgg tccgtgacct acatgtgctt
    24001 ctgcaacgaa taatctgtta gcatctaatt cgtgattgtt ctccgcatta gctaccgctg
    24061 attttaaaac tttgcttata acctcagcag catctcttgg agtatatttt aaaattgcga
    24121 aagcttcgtt tacgttcttt cctcttatta aatcaagaac aactcctact ttagttggag
    24181 acattcttac atatttagct atagctttag cttccatcta aagatcctcc tttcccggat
    24241 actattattt tctctttgat gttttttcgt ttttaatatg acctttgaaa gttcttgtta
    24301 aaacgaattc tcctaattta tgtccaacca tatcttctgt aacataaact gggatatgtt
    24361 ttcttccatc atgaacagct attgtatggc ctatcatttg tgggaatata gttgagcttc
    24421 ttgaccaagt tttgataact ttcttatctc cattttggtt catttcttct attttcttta
    24481 aaagacctgc atgcacgaaa ggtcctttct taattgatct actcactaga atttcctccc
    24541 ttcataaact taattatgcc gccaaactgt gaagagaata tttaattctc tccaaactat
    24601 ttagcgtttc ttctcttaat gatgaatcta tctgagtatt ttttgttctt tctagttttg
    24661 tatccaagtg ctggtttacc ccatggagta actggacttg gtctaccgat aggagatcta
    24721 ccttctccac caccgtgagg gtgatcgcaa gggttcatta cagaacctct aactgttggt
    24781 ctccatccca tgtgtctctt tctaccagct ttaccgatat ttacgatctc atgagttgtg
    24841 tttgatactg ttccaacagt agctttacat tcgattctta cgtatctcat ttcacctgat
    24901 ggtaatctta atgtagcata gtttccttct ttagccatta actgagctga agtacctgct
    24961 gatcttacca tttggccacc ttttccagct tgtaactcta tgttatgaac aaaagtacct
    25021 actggcatgt gttttaattg aagagcgtta cctggcttga tatcagcctc tggtcctgat
    25081 actactgtat ctcctacttt taaacctaca ggagcaagta tgtatctctt ctctccatca
    25141 gcataaacaa caagtgctat gtaagctgat ctgtttggat cgtactctac tgtagcaact
    25201 tttgctggaa ttccatcctt atttcttttg aaatctatta ttctatattt tctcttagct
    25261 ccaccaccat ggtgtctaac agttatttta ccttgtgcgt ttctaccacc agttttcttt
    25321 aaagaaacaa gaagtgactt ctctggtgca tttgtagtaa cttcttcaaa tgttggcata
    25381 gtcatttgtc ttcttgatgg tgtagttggg ttaaactttt taagtgccat tattgtccct
    25441 ccttcttata gcttatctgg ccttcaacca atacctgctt tttattattg cattccttcg
    25501 aagaattcga tgctgctacc ttctgctaaa gttataacag cttttttgaa gtcagctctt
    25561 tttccgatat gaactccaac tctcttagtt tttccttcga atctcatagt cttaacgtca
    25621 gcaactttta cgttgaaaac ttcttctaca gctctcttta tttgtacttt gttagctgcc
    25681 atatgaacta tgaaggtgta tttattttca gccatagctg ccatactctt ttcagttata
    25741 actggttttc tgattatatc atggcttgtt aacttcatta tgcgtacacc tcctcaattt
    25801 tgtttacagc ttctttagtt atcattaatg ttttgcagtt taataaatca taaacattga
    25861 tgttgttaac aggcataact gttacacctt ctatgtttct tgctgattta tatacatttt
    25921 cattaacttc agctgttatt attaatgtct tcttagcttc gaaagcattt aacattttaa
    25981 ctgcttcctt agtttttgga gcttcgaaat ttaagtcttc taaaacaacc atatctgcaa
    26041 ctttgctagt taaagcagaa gtcatagcta cttttctcat gctctttggt attgacattc
    26101 tgtaatctct tggttttggt gcaaatacaa ctccaccttt gatccattga ggcgctctga
    26161 tagaaccttg tcttgctcta cctgttcctt tttgtctcca aggcttgatt ccgcctcctc
    26221 ttacctcaga tcttgtttta gctgattgag taccttgtct cttgttagct aattgagcta
    26281 ctactacttg gtgtaaagca tatttgttta cttctactcc gaatacttgc tcatttaatt
    26341 ggatatctcc aacttgttgt ccttctttat taaataaacc tactttaggc attacgcttc
    26401 ctcctttcta cagaaattaa gccttaacgc tgtttcttat aactattgtg cctttatttg
    26461 gacctgggat tccccccttg attaagatga tgttcttttc aggcattatc ttaacaactt
    26521 ctaagttaag aacagttgtg ttcttagctc ccatatgtcc aggcattctt ttgttcttga
    26581 aagttcttga tgggtctgaa gcagctccca ttgaacctgg tgctctgtgg aatttagaac
    26641 cgtgagacat tggtcctcta ctagcattcc atcttttgat tgtaccttgg aatcccttac
    26701 ctttagaaac tcctgatacg tctactttgt ctccaatttc aaatgtgtca acctttattt
    26761 cgtctccaac attgtaatta gcaacatctt ccattctgaa ctcttttaaa gttcttctta
    26821 aagaaacacc tgctttagca aaatgtcctc ttcttggttt gttaactaat ttttctctta
    26881 attcaccgaa tccaacttgt attgcgtcat atccatcagt ttcaactgtt ttcttttgaa
    26941 ctacaacaca tggaccagct tcaactacag ttacagggat tactctacca ttctcatcga
    27001 atatttgagt cattccaact tttttaccta ttatagcttt tttcatgtgg ttacacctcc
    27061 taaacatatt agcggattgc ctctgcaatc ataacagttc aatctcttaa ttcaatctaa
    27121 ataaactatt aaagttttat ttcgatatca acacctgctg gtaagtttaa tctcattaat
    27181 gcatcaacag tttttggtga tggattaacg atgtctatta atctcttatg agttcttact
    27241 tcaaactgct ctcttgagtc tttgtactta tgtacagctc ttaagattgt tataacgtct
    27301 ttttcagttg gtagtggaac tggtccaaca acctttgctc ctgtagactt agcagtttca
    27361 acaattttct cagctgattg atctaatatt gtatgatcaa atgcttttaa tcttattctt
    27421 attttttgtt ttgacatttg gtttccctcc ttttcatgta cgctttactt ctaacgcaca
    27481 acagtaagca ttgtgtaaac tgtccctggt gtttcacact ttagacatgc gcacgcaacc
    27541 cctgtcgtcg gattctagat cctaacatgc tcatcaagaa ttacccggaa gtccggcaac
    27601 ctcttgattc atcgctgttc gctttacaac ttcttcatta tactatgtat attatgtttt
    27661 ttcaagtatt tttttagaag ttttaacata tacattgatg tactatcttt accccaaaac
    27721 cgttcaaaaa aataaagcac taatattaat aatatactat ttttttaatt tttcaacaag
    27781 aattttattc ctaaaaaaat tttatattat tatt
//
LOCUS       DAEVGI010000019        27240 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEVGI010000019 DAEVGI010000000
VERSION     DAEVGI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 27240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27240
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.019"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(133..1311)
                     /locus_tag="K8M77_000767"
     CDS             complement(133..1311)
                     /locus_tag="K8M77_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBI7085282.1"
                     /translation="MREVVIASAVRTALGSFGGSLKDVPAVDLGALVIKEALNKAGVK
                     PECVDEVLMGNVIQAGLGQNPARQAAVKAGLPVEIPSMTINKVCGSGLRCVALAAQMI
                     KAGDADVIVAGGMENMSQGPYVLKTARFGQRMGDGKMVDAMVNDALTDAFNGYHMGIT
                     AENIAEQWGLTREMQDEFAANSQIKAEAAIKNGKFKDEIVPVVIPKRKGDPIVFDTDE
                     FPRFGTTAEKLAKLRPAFKKDGTVTAGNASGINDGAAALVVMSAEKAKELGVTPICKI
                     VSYGSKGLDPSIMGYGPFYATKKALEGTGLTVADLDLIEANEAFAAQSLAVAKDLEFD
                     MSKVNVNGGAIALGHPVGASGARILVTLIHEMMKRDAKRGLATLCIGGGMGTALIVER
                     "
     gene            complement(1579..2733)
                     /gene="wecB"
                     /locus_tag="K8M77_000768"
     CDS             complement(1579..2733)
                     /gene="wecB"
                     /locus_tag="K8M77_000768"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBI7085283.1"
                     /translation="MEKIKVLTIFGTRPEAIKMAPLVKELEKRPEIESKVCVTAQHRE
                     MLDQVLELFDIKPDFDLDIMKNRQTLTGITNRVLEGLEKVFTEEKPDMILVHGDTTTT
                     FAGGLAAFYQQIRVGHVEAGLRTFDKYFPFPEEMNRKLTGALADLHFAPTKGSKANLL
                     REGINENDIKITGNTVIDAMEHTVEDNYVFENDELNKIDFENKKVIMITAHRRENWGE
                     GIENICTALNKIVEDNKDVELVYLVHLNPVVKDVVYKNLDGKERVHLLPPLDTKETHN
                     LMNKCFMVMTDSGGLQEEAPHLGKPVLVLRDVTERPEAVQSGTVKLVGTDVEKILEEA
                     NSLINDEKAYAKMSKAINPYGDGKASERIVKAILNYFGKNDEEVEEFISK"
     gene            complement(2797..3426)
                     /gene="upp"
                     /locus_tag="K8M77_000769"
     CDS             complement(2797..3426)
                     /gene="upp"
                     /locus_tag="K8M77_000769"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBI7085284.1"
                     /translation="MSKVTEITHPLILHKLAFIRDKNTGSKDFRELVSEVSMLMAYEV
                     TRNLPMEEIEIETPVCKTKCKVLAGKKVAIVPILRAGLGMVDGMLQLIPAAKVGHIGL
                     YRDEETLQPVEYFCKLPQDIAERDIIVVDPMLATGGSATDAITLLKKRGAKQIRLMCL
                     ISSPEGIEFVQKAHPDVDIYVACIDEKLNDHGYIVPGLGDAGDRLFGTK"
     gene            complement(3471..3920)
                     /gene="rpiB"
                     /locus_tag="K8M77_000770"
     CDS             complement(3471..3920)
                     /gene="rpiB"
                     /locus_tag="K8M77_000770"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBI7085285.1"
                     /translation="MKIAIGCDHGGFNLKNTVIKHLEEKGIEVKDFGTYSTDSCDYPD
                     ISIQVAEAVAKKEFEYGILICGTGIGISIAANKVPGIRAALCSDTFSAHATRQHNNAN
                     ILAMGERVVGPGLALDIVDTFLNSEFEGGRHQNRIDKITAIEEKYSK"
     gene            complement(4184..5236)
                     /locus_tag="K8M77_000771"
     CDS             complement(4184..5236)
                     /locus_tag="K8M77_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBI7085286.1"
                     /translation="MKTKVQFIKDIDKDIKYLEEAAEIIKKGGIGAFPTETVYGLGAN
                     ALDGEAVKKIFVAKGRPQDNPLIIHIADKKDIDKYVAEIPKYAQELMDKFWPGPLTII
                     LKKKDIVSDVTSAGLDTVGVRMPNDEIALKLIELSGVPIAAPSANISGRPSPTDIERC
                     EEDLNGRVDFIIGAKKSEIGVESTIVDATGEHLCVLRPGGITLEMLKDIDEGVYIDKA
                     ILTTLDKDTKPKAPGMKYKHYAPKAKVRMVQGPINKMVEKIKEIVHNYIDEDLKVGIM
                     ATDETISMYNEGIVKSLGTREDLTSVTRNLFETLRAFDDLEVDIIITEAFEEKDLGVA
                     IMNRLKKSCGFDITIV"
     gene            complement(5259..5849)
                     /locus_tag="K8M77_000772"
     CDS             complement(5259..5849)
                     /locus_tag="K8M77_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085287.1"
                     /translation="MDIDKAIKKQKKSYKRFMFVMVILLILLPLLVCFFEVYKDFLIV
                     YLIFLEIFIVIVMIKNFNNIRLEYTCENGILRIKPNVLSKYKYIYCDRVGLVHTENEL
                     EDMKIIIVFANKGRKGKFGKQVGEYFCKVHPGLSKDYNKMIKLNKETQWYYIIIKRGA
                     LKKFKLLDDIYKNCVTATYTNDSIENIKIARGQKNL"
     gene            complement(5928..7010)
                     /gene="prfA"
                     /locus_tag="K8M77_000773"
     CDS             complement(5928..7010)
                     /gene="prfA"
                     /locus_tag="K8M77_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBI7085288.1"
                     /translation="MILDRLNFIENKYDELSVKISDPSIMANQKEWRKLCKEHADLEV
                     IVNKYKEYKEATEELEANKEMLSEESDQEMREMINSEIKDLTERKKELEDEIQILLLP
                     KDPNDDKNVFVEIRGGAGGDEAALFAANLFRMYTKYAEKNRWKVELMSANETDIGGFK
                     EVVFMIKGAGAYSKLKYESGAHRVQRVPDTESSGRIHTSTATVAVLPEVDDVEIEIND
                     KDIKIDVFRASGNGGQCVNTTDSAVRITHLPSGLVVSCQDEKSQLKNKEKAMKVLRAR
                     LFEQAEAERLAGIAEDRKSQVGTGDRSERIRTYNYPQGRVTDHRINMTLYKLDSFLEG
                     DIDEILNALITEDQAQKMKAMGNTEF"
     gene            complement(7067..8830)
                     /gene="prmC"
                     /locus_tag="K8M77_000774"
     CDS             complement(7067..8830)
                     /gene="prmC"
                     /locus_tag="K8M77_000774"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI7085289.1"
                     /translation="MRKCQVGGQAVLEGVMMRGSKGTATAVRTPEGDIEVSFEKTIPY
                     TKKNKILGLPFIRGFVTLIESLIVGLKSLNYSASFFDDTEPSKFEDWLNNKFGEKANN
                     VIMTLTIMLSFVFAIILFVAIPTGITFLLKKLNIPDWSLSAIEGIISIGMLLGYMYLM
                     GKVDDIERVFQYHGAEHKTIFCYENEDELTVENVRKYSRFHPRCGTNFLFLVAIVSIF
                     IFSFTKWDSVAQRTAIRVAMLPVISGITYELIKWLGKSQGNFAKIIAAPGLQLQKLTT
                     REPDDLQIEVAIASLRRAEGLKEPNKKVGELLNLGNETLKEVGIDTYILDTQLLLGKI
                     LEKDKIWLITNKNEEVKKSDEIHFLNLLEKRKSKMPMQYILGTCEFMGLDFYVEEGVL
                     IPRGDTEIIVEEVLNNIDEDAEINVCDLCCGSGAIGLSLANYRKNIIVDLVDIDDIPE
                     KVTRKNIRELELSKRCGFIKSDLLSEVIKKGNKYDILVSNPPYIRTEVINTLMEDVKD
                     YEPHLALDGGEDGLIFYRRIIDESLEVLKENGILAFEIGHDQGEDVKNLMIEKGYYDV
                     KVIKDLAGLDRCVIGRVSLER"
     gene            complement(8856..9455)
                     /locus_tag="K8M77_000775"
     CDS             complement(8856..9455)
                     /locus_tag="K8M77_000775"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI7085290.1"
                     /translation="MSKLYFRYGAMNSGKSTHLMQVAHNYEERGMEVIIIKPSTDKKG
                     GDKVVSRLGVERVVDILIDNDENIMETIEDRLEKGRKIDCIIVDEVQFLKAHHIDELF
                     EIAVGKGIPVICYGLRTDFQMNGFEGSERLLLLAHSIEEMKTICRCGRKAILNGRMIN
                     GKFTFEGEQVAIDLVDNVEYQSLCGQCYFKYKYGKDSDK"
     gene            9611..9817
                     /gene="rpmE"
                     /locus_tag="K8M77_000776"
     CDS             9611..9817
                     /gene="rpmE"
                     /locus_tag="K8M77_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBI7085291.1"
                     /translation="MRQGIHPEYHHDTVVKCACGNTFTTGSVKPELKVEICSKCHPFF
                     TGKQKIVDVGGRVDKFNKRFNLNK"
     gene            9926..11050
                     /locus_tag="K8M77_000777"
     CDS             9926..11050
                     /locus_tag="K8M77_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBI7085292.1"
                     /translation="MKKVLILSTSTGYGHNQAANSLMELIKNDDTEILVHDFLKENRF
                     FDRSIVNGYDLCASSLGTLYGLLYKISNIKFINNLVSFLFLPVANKLVKFIHSFNPDL
                     IITTHPLAVSILSYLKKRQIIKVPVISVVTDFKCHYTYVSKIIDHYIVACDFTKENLA
                     SKGIPKERISPFGIPVKQDFYKEDYHNYIENIIQSPLNILLMGGGMGLDNISKVLKTL
                     IKNDNPLNLTIVCGNNAELKKELCKEYGHITGNKNLNILGYTTEIPKIMKSSDLIITK
                     PGGLTTTESLLSHLPMIIPFIIPGQESENREFLSKSNCAITINHLEELNKVINDLNKD
                     NNKLINMRQSILDVLSSYSPEETIKLCTKMLNDSYNKRRY"
     gene            complement(11093..12553)
                     /gene="rho"
                     /locus_tag="K8M77_000778"
     CDS             complement(11093..12553)
                     /gene="rho"
                     /locus_tag="K8M77_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBI7085293.1"
                     /translation="MNIEQYENMTLVQLKEQAKELGIKNISKKKKSELIEELKAEFNK
                     QNSNTKVIHKDGVILREKISQKEQGGNKESFSGERVVRNSNNYNNNNSNYNNSNDEGK
                     KEQLKDMISSSDSAKGILEILDNNNFGFLRCRNYLTSEDDIYVSPSQIRRFGLRTGDE
                     VQGKVRIPKDGEKFKALLYVERVNGESPEKAVGRKKFEELTPIYPKERLRLETENGRD
                     LSSRLMDIICPIGKGQRGMIVAPPKAGKTTLLKRIAQNISKNNPEVKLIVLLIDERPE
                     EVTDMKRSIDGEVIYSTFDEEPQNHAKVSSIVLERAKRMVEQGRDVVILMDSLTRLSR
                     AYNLTVTPSGRTLSGGLDPGALIMPKKFFGAARNLEEGGSLTILATSLVDTGSRMDDM
                     IFEEFKGTGNMEVHLDRKLQERRIFPAIDIYKSGTRKEDLLFNEEEREASYKIRRVLQ
                     KENNIEDVAEKLINLLSKTKNNKEFLQVVLKSNLEN"
     gene            complement(12816..14423)
                     /locus_tag="K8M77_000779"
     CDS             complement(12816..14423)
                     /locus_tag="K8M77_000779"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBI7085294.1"
                     /translation="MSKNTKYVFVTGGVVSSLGKGITAASLGRLLKNRGLSVSIQKFD
                     PYLNIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDENLTQNSNVTSGRVYWSVI
                     SKERKGEYLGGTVQVIPHITNAIKDRVHRVGKERDVDVVITEIGGTIGDIESLPFLEA
                     IRQIKYDVGKENVCFIHVTLVPYLGKAGELKTKPTQHSVKELRSIGIQPDIIVCRSEK
                     ELSEDIKKKIGLFCNIDASEVIQNLDAEHLYAVPLMLHKEGLDRLVCEKLGLGCRDID
                     NAEWIDMVHRITHLTHTTKIALVGKYVELHDAYISVVEALNHGGLSNDTNVEIEWINA
                     EDVTKENVDELLSGVDGVLVPGGFGDRGVEGKIEAIRWARENKKPFLGICLGMQCAVI
                     EYARNVLGLEGAHSSELNPETPFPVIDLMPEQKDVEDLGGTMRLGLYPCKLEDNTFCK
                     DVYASDLIYERHRHRYEFNNEYRTQLIESGLTIAGTSPDGRLVECVEVKDHPWFVAVQ
                     YHPELKSRPNRPHPLFVGFVGAALNNK"
     gene            complement(14763..15182)
                     /locus_tag="K8M77_000780"
     CDS             complement(14763..15182)
                     /locus_tag="K8M77_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBI7085295.1"
                     /translation="MIKKAIISICSTQDIGEKEKIEVVTVGEFSINGDEFIATYDETE
                     ISGMEGTKTTLKIKGNKVVLHREGTTSTKMEFQKNNTQVALYNTPYGMLELKTSTKEL
                     DLDVDEKGGNISIKYHLIAGGQEPIKTNLDMKIKVED"
     gene            complement(15205..16185)
                     /locus_tag="K8M77_000781"
     CDS             complement(15205..16185)
                     /locus_tag="K8M77_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBI7085296.1"
                     /translation="MTRALAMISGGLDSILAAKLIKDQGIEVIGICFKSYFFNEENAK
                     RMCKQIDMPLEVVDFSEEHFEMVKDPKHGRGKNMNPCIDCHAMMMRYSGELLKKFDAD
                     FIITGEVLNQRPMSQNRQALNTVKKESGFSEKILRPLCALNLEPTEMELNGLVDREKL
                     LKISGRSRKTQMELAEKWNIVDYPSPAGGCKLTEPGYAIRLSDLLDNQETVDKDEIEV
                     LKYGRHMRISPKNKVIVARNGEEWKEIVKFKREDDILVTSKNSTGAPVILRGEFNKED
                     LEIAARICGRYCKEKDSDAVEINYEKNGEINTITITPFKDEELKKYMINQ"
     gene            complement(16232..16861)
                     /gene="spoIIR"
                     /locus_tag="K8M77_000782"
     CDS             complement(16232..16861)
                     /gene="spoIIR"
                     /locus_tag="K8M77_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBI7085297.1"
                     /translation="MKKILSVLFLGFICLFIVGSSLNTRQALGVTEENIVEDISEKLI
                     RFHVLANSDSDIDQDLKLRVKDEVLKYISPILNESQSLEESREILKREDKNIIKIAED
                     YIKSQGFDYTVETTLTRENFPVKEYGNIVLPQGEYEAYRILIGEGKGKNWWCVMFPPL
                     CFIDVTKGQVAYDETEKKMKDVLSEEEFKSVNKKENNVNFEFKVIDLFK"
     gene            complement(16930..17796)
                     /locus_tag="K8M77_000783"
     CDS             complement(16930..17796)
                     /locus_tag="K8M77_000783"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBI7085298.1"
                     /translation="MKMKAYAKINIALDAIGKREDNYHLLRMIMQTVDLYDVIDIEKS
                     NDSNISISCNKHYVPTDERNLGYKAAVLFRDEFNIKNGVKISIKKNIPVAAGMAGGST
                     NAAAVLVIMNKLFNVNASLEVLKEIGLKIGADVPYCIEGGTALCEGIGEIITPLKPFE
                     NKILVVLKPNFGVSTKEVYTNLDINKIRKHVNIEGLIQAMENDDLDYVSKNMKNVLEN
                     VTLKKHTILKNIKEDMRKSGALGAMMSGSGPTVFAFFDDMLTAQRAFEFLKGKYKYSD
                     VYITRTINSNNL"
     gene            complement(17932..20556)
                     /gene="cphA"
                     /locus_tag="K8M77_000784"
     CDS             complement(17932..20556)
                     /gene="cphA"
                     /locus_tag="K8M77_000784"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HBI7085299.1"
                     /translation="MKINNQRIFEGRNIYSHRKCIRLDVDLEGYCETPTKDIDGFNER
                     LLSYVPELYVHRCGIDIEGGFVQRLKEGTYLAHVCEHTIIGIHNKLGIDIKYGKAREI
                     KDDFYYIIFEYKLKKTAILIAELAIDLINSIIKKKDINFDERIEIIKRVIMEESIGPS
                     TKAICDAAKEVGLPIITVGNGNLYQIGYGKKGKRFSATIGNNTKGIAIDIACDKILTK
                     ELLDIQNLPVARGEKIFNTIHLLEVVNRIGYPVVLKPQWGSKGNGVFVNINSEKELLR
                     AYAEITKECKEIMMEEYKVGNDYRVMLVDYKVAAVSLRKPPYITGDGVRNIRDLIEAM
                     NANPLRGEGHEKPLTKVKIDEELINMLSKLGYSLNSVLEYGEKVTLRGNANLSTGGSA
                     EDYTDLICKENIEICERAAKTIGLDICGIDICAKSISEPLYENDGIILEVNAAPGIRM
                     HHFPTIGKERNVGRKILDNMFKNNYSNIPVISVTGTNGKTTTVRLISYVLNLIGFNVG
                     CTTTSGVKIGNKYIHKGDDTGYNSARSVLLNPEVDIAVLESARGGLVRRGLAYDLADV
                     GVITNIREDHLGIDGINDMEDLSFVKSLVGEAVKDNGYSVINADDEWSLKVLDRIKKP
                     KILFSMNENNKYLQENLKLENPIVFYRDETIYVKNRGKEYKIASAEDMKFTMNGKLKH
                     NIENAMAACAALVGIEVDYCIISKGLKKFKSCEEDNRGRFNMFDVNGVNVILDYGHNI
                     DGYKVVIDSLKNLGFKNITGIIGVPGDRDLNTMKEVGKISGDFFDSIVIKEDKDLRGK
                     DKGEVASIINEGVLSSNRKDLNVKIILSEEEALRETLKLAKKGETIIMFFEDYESLYD
                     IINEFKNKGTKDLKSASI"
     gene            complement(20543..21370)
                     /locus_tag="K8M77_000785"
     CDS             complement(20543..21370)
                     /locus_tag="K8M77_000785"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HBI7085300.1"
                     /translation="MKGSSKKNLIIIGGAEDKKGEKIILNFVADKFKESEGIMLIATI
                     ATESPNIVIEQYKNIFSSMGIREIKELRVIEREEAFDENNISLVNEASVIFFTGGDQL
                     RITSLIGGTPLYDTLQKRSNEGCYFVGTSAGASVMSDVMIVGGEEGESPKKSSLNMSA
                     GLGLIKNTLIDQHFAQRGRIGRLLSGVCQNPEVLGIGIDEDTAIVINDDELTVIGSGA
                     VYFVDGRNIEYTNVSEKRQDEVFSIFNIKLHVLTAGKKMNLSTRVPFEEERNKNEDK"
     gene            complement(21470..23032)
                     /locus_tag="K8M77_000786"
     CDS             complement(21470..23032)
                     /locus_tag="K8M77_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBI7085301.1"
                     /translation="MVNLDIIKESLGFEKVLSENSLDIPFREEYLIPDTQPDVHDVLS
                     VDTKVYVINREVQSDRVLLEYEVECNIMYLAREEEGLGINSVIYKEKNSSFIDIAGAE
                     HSMMCDMECDLEHIGANIINERKIRVEGKLRTKYRVYKEEKIDVVKELDGLGDLQIKR
                     KPESIEKNITNMVCSMNGELPINVGMDKPQIGKIIKCGYMLHRQEIKLGEDKVQASCL
                     AKINILYRAYDSRDVVALEEDLYLSNDEEVVGVNSDMNCNGYFEIKGHNVNISQNDLG
                     ESRIIDVNLSVDANVSISKLEFAEIVEDLYSPTQNIMPLKEVCKINMAVSENSNESVI
                     KGNIDLGKNGVDATHTVDCKGKIVDMESYFVDQKVVIKGNLSVESIYKTTDEEKGLNK
                     VNGEIPFEVALDMPNSQSGMTFDTKANLEDLQCSIEGNILAVKAVVYFYAKCSDVFDK
                     EYVKDIEQNDDTVEKKASITIYTVQKGDTLWDLAKKYKTTIDTLVKLNDIEDADSIYV
                     GDKIMIPGRAVL"
     gene            complement(23388..23624)
                     /locus_tag="K8M77_000787"
     CDS             complement(23388..23624)
                     /locus_tag="K8M77_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBI7085302.1"
                     /translation="MSTRLTLNSIRKSIEDHVGEKVKLRANGGRKKILENEGILESVH
                     PSIFVVRLQKDTQRKVTYSYSDVLTKTVQLDFVG"
     gene            complement(23787..24668)
                     /gene="yabG"
                     /locus_tag="K8M77_000788"
     CDS             complement(23787..24668)
                     /gene="yabG"
                     /locus_tag="K8M77_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBI7085303.1"
                     /translation="MKVGDLVVRKSYSKDITFKIIDIKENEEGITYILKGIHIRIIAD
                     SKGEDLEKVEDAFAGDKDKYFDSKMNDIIKKVILSREQKNNQLMTRGSEDDNLKRTEK
                     GLVFGRPGRILHIDGDPSYMETCLKAYKQLNLNAVGIAVSEREQPLKILSLVKEYNPD
                     IVVLTGHDGVLKDSKGMLDLNNYRNSKYFIESVKILRNYNSSYDELVIFAGACQSCYE
                     SILEAGANFASSPNRVLIHCLDPVLVCERVAYTRIDEIVSIKDVIENTITGIKGVGGL
                     QTRGKYREGYPKSLYLN"
     gene            complement(24875..25876)
                     /locus_tag="K8M77_000789"
     CDS             complement(24875..25876)
                     /locus_tag="K8M77_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HBI7085304.1"
                     /translation="MMREFEIERQFDIKIEKLKPSKGVYYLKSNKGDRCLKRINYGTQ
                     KLLFVYGAKEHLAKNGFEHIDRYFLNIEDEPYALVNEDLYTLSNWIKGRECDFTNIEE
                     VKLAAKKLAELHEASKGYDPPENSKLKSDLGRWPYLMEKRGKALEKMRGMARKKNLKK
                     DFDIIYIKNVDFYKELAIRATKILNNSKYLSLCEEAEAEKVFCHHDYTYHNIIIGDDN
                     EVYIIDFDYCKREIRTYDIANFMKKVLKRVDWNIEYAEAIINAYNTVSPLREEEYEVL
                     YAYLLFPQRYWRLANRYYYNEVMWGQNIFINKINNIINEKESYMKFIEEFKSKYNQA"
     gene            25985..27112
                     /locus_tag="K8M77_000790"
     CDS             25985..27112
                     /locus_tag="K8M77_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI7085305.1"
                     /translation="MKICIDGRAATLYRGTGIGNYTYQIINNLHQIDFLNEYNILTPE
                     ASSLKLPKKNNFNYLSSSTNDKKNFWEFINTKNPKENIIGDVYHIPQNGIGFSKPNDI
                     KTVITLHDIIPMKMPDTVSETFLKIFNENIQNILDNTDGIITVSNFSKEDISKTFSYP
                     KEKIFVTHLAAEEIYTPLNKFHSSQYLKKHYGIDRDFLLYVGGFSPRKNILGLIDAFN
                     LVKNSYKRDLKLVIIGTKGPSYEIYRKKVDELNLSSSVIFTGFIPIDDMPIFYSASKA
                     LVYPSFYEGFGLPPIECMACGTPVIASNLTSMPEVCQDAALLVDPYDVDEIKENILTL
                     LNNHKFYSLMIYKGLSHSSKFNWKKTAYNTLEVYKHIYSQI"
BASE COUNT         8465 a         5029 c         2721 g        11025 t
ORIGIN      
        1 ctcaaattag tgcatttttt aaagatattt ttaaaattaa acataaattt taaataattt
       61 tccacaaaaa aagatactat cccaagataa gatagtatct ttaaaatata tattataata
      121 atggtaaact tattatcttt ctactattaa agcagttccc attcctccac ctatacaaag
      181 tgttgctaaa cctctcttag catctctctt catcatttcg tgaattaatg taactaatat
      241 tctagcccct gaagctccta ctggatgtcc taaagctata gctccaccat ttacattaac
      301 tttactcata tcaaattcta aatctttagc aactgctaaa ctttgagctg caaaagcttc
      361 atttgcttct attaagtcaa gatctgcaac tgttaatcca gttccttcta aagctttttt
      421 agtagcatag aatgggccat atcccataat tgaaggatct aatccttttg aaccataaga
      481 aactatttta caaattggag ttactcctaa ttcttttgct ttttcagcac tcataacaac
      541 taacgcagca gcaccatcat ttatacctga tgcattacca gctgtaactg taccatcttt
      601 tttgaatgct ggtcttaact tagctaattt ttcagcagtt gttccaaatc tagggaattc
      661 atcagtatca aatactattg gatcaccttt tctttttgga attactactg gaactatttc
      721 atctttgaat tttccattct ttatagctgc ttcggcttta atttgagaat ttgcagcaaa
      781 ttcatcttgc atttctctag ttaatcccca ttgttctgct atattttcag cagttatacc
      841 catatggtat ccattaaatg catctgttaa agcgtcattg accatagcat caaccatttt
      901 tccatcgccc attctttgac caaatcttgc agtttttaaa acatatggcc cttgtgacat
      961 gttttccata ccaccagcta ctattacgtc agcatctcct gcttttatca tttgagctgc
     1021 taaagccaca catcttaaac ctgaaccaca aactttattt attgtcattg aaggaatttc
     1081 aactggtaaa ccagctttta cagctgcttg tcttgcaggg ttttgtccta atccagcttg
     1141 aattacattt cccattaaaa cttcatcaac acattctggt tttacaccag ctttatttaa
     1201 agcttcttta ataactaaag ctcctaaatc tacagcagga acatccttta atgaaccacc
     1261 aaatgaacct aatgctgttc ttactgcact tgcaataact acctctctca tatcaaaaac
     1321 ctcccattta ggtaaaaatt tatcaaaaat ctatattttt tctaactaat ttaattataa
     1381 ccataaattt actagctttc aatatttttt gttaaatttt aaacaatctt ttttattatt
     1441 ttttctcttt tcgaaaaaaa agacagataa tttagcttta ttatagaagt cttttattaa
     1501 aatttctata atattctaaa tatctgtcta tttataccat ttattcaaat tatatttatt
     1561 aaacttttta acaaagtctt atttgcttat aaactcttct acttcttcat cattttttcc
     1621 aaagtaattt aaaatagctt ttactattct ttcagaagcc ttaccatctc catatggatt
     1681 tatagcttta ctcatttttg cataagcttt ttcatcattt ataagcgaat tagcttcttc
     1741 taatattttt tcaacatcag taccgactaa tttaactgtt cctgattgaa cagcttctgg
     1801 tctttctgta acatctctta aaactaatac tggttttcct aaatgaggag cttcttcttg
     1861 taatccacct gaatcagtca taaccataaa gcatttattc attaagttat gagtttcttt
     1921 tgtatcaagt ggtggtaaaa gatgtactct ttctttacca tcaagatttt tataaacaac
     1981 atcttttaca actggattta agtgaacaag gtaaactaat tcaacatctt tattatcttc
     2041 aactatttta tttaaagctg tacagatatt ttctatacct tctccccaat tttctcttct
     2101 atgtgcagtt atcattataa cttttttgtt ttcaaaatct attttattta attcatcatt
     2161 ttcaaaaaca tagttatctt ctacagtatg ttccatagca tcgattacag tgttacctgt
     2221 aatttttatg tcattttcat ttattccttc tcttaaaagg ttagcttttg atcctttagt
     2281 tggagcaaag tgtaaatcag ctaaagcgcc tgttaacttt ctattcattt cctctgggaa
     2341 tgggaaatat ttatcaaaag ttctaagtcc agcttcaaca tggcctactc ttatttgttg
     2401 atagaatgct gctaatcctc cagcaaaagt tgttgtagta tcaccatgta caagtatcat
     2461 atctggtttt tcttctgtaa atactttttc taatccttct aaaactctat ttgttattcc
     2521 agtaagagtt tgacgatttt tcattatgtc tagatcaaag tcaggcttta tatcaaatag
     2581 ttctaaaact tgatctaaca tttctctatg ttgagcagtt acacaaacct ttgattctat
     2641 ttccggtctt ttttctaatt cttttactaa tggagccatt tttatagctt ccggtcttgt
     2701 tccaaagata gttaaaactt ttatcttttc catgaatcat ttacctccaa aaataattaa
     2761 tagctactta aaattaagta gctatttaaa ttataactat tttgttccaa atagtctatc
     2821 tcctgcatca cctaatcctg gaacgatata tccatgatcg tttaattttt catcaataca
     2881 tgcaacatat atatcaacat ctggatgagc tttttgtaca aactctattc cttctggtga
     2941 agaaattaaa cacataagtc ttatttgctt agctcctctt ttctttaaaa gagttatagc
     3001 atctgtagca gagccacctg tagctaacat tggatcaaca actattatat ctctttcagc
     3061 tatatcttgt ggtaacttac agaaatattc tacaggttga agagtttctt catctctgta
     3121 tagtcctata tgccctactt ttgctgctgg gataagttgt agcataccat ctaccatacc
     3181 taatccggct cttaatattg gaactatagc gactttttta ccagctaaaa ctttacattt
     3241 agttttgcaa actggagttt ctatttctat ttcttccata ggaagatttc ttgttacctc
     3301 ataagccata agcatagaaa cttcacttac tagttctcta aaatccttag atcctgtatt
     3361 tttatctctt ataaatgcta acttatgtaa tataagtgga tgtgttattt ctgttacttt
     3421 actcattgta tcttcctcct aaaatttata taaaaatatt ttttatcaaa ttattttgaa
     3481 tacttttctt caatagctgt tattttatct attctatttt ggtgtcttcc accttcaaat
     3541 tctgaattta agaaagtatc aactatatct aaagctaatc ctggtcctac aactctctca
     3601 cccatagcta gaatatttgc attgttatgt tgtcttgttg catgagcact aaatgtatca
     3661 gaacataatg ctgctcttat tcctggaact ttatttgctg ctatagatat tcctatacca
     3721 gttccacaaa ttaatatacc atattcaaat tcttttttag ctacagcttc tgcaacctgt
     3781 atagatatat cagggtaatc acaagaatct gtgctgtatg taccaaaatc ttttacctca
     3841 ataccttttt cttctaaatg ttttatcact gtgtttttta aattaaatcc accatgatcg
     3901 caaccaattg ctattttcat ttcattaacc tccattatac ctgtttgttt taatatatta
     3961 ttttaaaaaa agtgcttttt caatgactaa taacactact taatttaaaa aattaaatag
     4021 taagtcaaat aaaaaagcac tttttttatt aactggaaga acactaaata aaggtattaa
     4081 actttcaaaa aaaacaaata tagaattttt aaaaaatata aattcaaaac agttcctttt
     4141 tttcaaaagt ttcattctta tctagctcct tccatatata ttcttaaact attgttatgt
     4201 caaatccaca ggattttttt aatctattca ttatggctac gcctaaatct ttttcctcaa
     4261 atgcctcagt tattataata tctacctcta agtcatcaaa agctctaagc gtttcaaata
     4321 aatttcttgt tacagaagtt aagtcttctc tagttcctaa tgattttact attccttcat
     4381 tgtacataga aattgtttca tcagttgcca taattccgac ttttaaatct tcatctatgt
     4441 aattatgcac tatttcttta attttttcaa ccattttatt tattggacct tgaaccattc
     4501 taacttttgc ctttggtgca taatgtttat acttcattcc cggtgcctta ggttttgtat
     4561 ctttatctaa agtagttaat atagctttat ctatatatac cccttcatct atgtccttta
     4621 acatctctaa agttatgcct ccaggtctta aaacacataa atgttcacca gttgcatcta
     4681 ctatagttga ttctactcct atttcacttt ttttagctcc tattataaaa tcaactcttc
     4741 catttaaatc ttcctcacat ctttctatat ctgtaggact tggtcttcct gatatattag
     4801 ctgatggtgc tgctattgga actcctgata gttctattag ctttaaggct atttcatcat
     4861 ttggcattct aactccaact gtatctaaac ctgctgatgt aacatctgaa actatatctt
     4921 ttttctttaa tattatcgtt aaaggacctg gccaaaattt atccattaac tcctgtgcat
     4981 atttaggtat ttcagcaaca tatttatcta tatctttttt atcagctata tgtattatta
     5041 aaggattatc ctgcggtctt ccctttgcca caaaaatctt tttaacagct tctccatcta
     5101 aagcatttgc gcctaatcca taaactgttt cagtaggaaa tgccccaata ccaccttttt
     5161 ttattatttc agcggcttcc tctaaatatt ttatatcttt atctatatct tttatgaatt
     5221 gtacttttgt tttcataaaa caatatcacc ttcatctttt ataaattctt ctgacctctt
     5281 gcgatcttta tattttcaat actatcattt gtataagttg ctgtaacaca atttttataa
     5341 atgtcatcta aaagtttaaa tttctttaaa gcccctcttt ttattattat ataataccat
     5401 tgtgtttcct tgtttaattt aatcatctta ttataatcct tgcttagtcc aggatgaact
     5461 ttacaaaagt actctcctac ttgctttcca aacttacctt ttcttccttt gttagcaaag
     5521 acaattataa ttttcatatc ttcaagttca ttttctgtat gaactaaacc tactctatcg
     5581 cagtatatat acttatattt agataacaca ttaggtttta ttcttagaat tccattttca
     5641 caagtatatt ctaatcttat attattaaaa tttttaatca taacaataac tataaaaatt
     5701 tctaaaaaga ttaaatatac tataagaaaa tccttataaa cttcaaaaaa acaaactaaa
     5761 agaggtaata atattagaag tattaccata acaaacatga atcttttata tgattttttt
     5821 tgttttttta tagctttatc tatatccata attatattcc tctttaatta ccattttaaa
     5881 actatgtatt tttaaagccc tacattattc atgtaaggct ttgtatatta aaattcagta
     5941 ttacccattg ctttcatctt ttgagcttga tcttcagtaa ttagagcatt taatatttcg
     6001 tctatgtcac cttctaagaa actatctaat ttatataaag tcatgtttat tctgtgatca
     6061 gttactcttc cttgtggata gttgtaagtt cttattcttt cacttctatc tccagtacca
     6121 acctgactct ttctatcttc agcaatacca gctaatcttt cagcttcagc ttgctcgaat
     6181 aatcttgctc ttaatacttt catagctttt tctttatttt taagctgtga tttttcatcc
     6241 tgacatgaaa caactaatcc tgaaggtaag tgagttattc ttacagctga atcagttgtg
     6301 ttaacgcact gaccaccatt acctgatgct ctaaatacgt ctattttaat atctttatcg
     6361 tttatttcga tttctacatc atcaacttct ggtaaaacag ctactgtagc agttgaagta
     6421 tgtattcttc ctgaactttc tgtatcagga accctttgaa ctctgtgtgc tccactctca
     6481 tatttaagtt ttgaataagc acctgctcct tttatcatga atacaacctc tttgaaacct
     6541 ccaatgtcag tttcatttgc actcataagt tcaactttcc atctattctt ttcagcgtat
     6601 ttagtgtaca ttctgaaaag attagctgca aatagtgctg cttcatctcc accagcacca
     6661 cctctgattt caacaaatac gtttttatca tcattaggat cttttggtag taaaagaatt
     6721 tgtatttcgt cctctaattc tttttttctt tctgttaagt ccttgatttc agagtttatc
     6781 atttctctca tttcttgatc actttcttct gaaagcatct ctttgttagc ttctaattct
     6841 tctgtcgctt ctttgtactc tttgtactta ttaactataa cttctaagtc agcgtgctcc
     6901 ttacaaagct ttctccactc tttttggtta gccattattg aaggatcact aatcttaact
     6961 gaaagttcat catatttatt ttcaataaaa tttaatctat caagtatcat ctattatcac
     7021 tccgtataca gattttcata ttttacaatt atagcacact actatgctat ctttcaaggc
     7081 ttactcttcc tataacacat ctatctaaac cagctaaatc ttttatgacc ttaacatcgt
     7141 aatatccttt ttcaatcata agatttttaa catcctcacc ttgatcatgt cctatttcaa
     7201 aagctaatat accattttct tttaatactt ctaaagattc atcaataatt cttctataga
     7261 atattaaacc atcttctccc ccatctaaag ctaagtgcgg ctcataatcc ttaacatctt
     7321 ccattaaagt atttatgact tccgttctta tatatggtgg attagaaact agaatatcat
     7381 acttatttcc ttttttaatg acttcactta aaagatcact cttaataaag ccacatcttt
     7441 ttgataattc taattctctt atattttttc ttgtaacttt ttctggtata tcatctatat
     7501 ctactaaatc tacaataata ttttttctat aattagctaa agataagcct atagctccac
     7561 ttccacaaca taaatcacat acattaattt ctgcatcttc atctatattg tttaatactt
     7621 cctctacaat tatttcagta tctcctcttg gaattaaaac tccctcttct acataaaaat
     7681 ctaatcccat aaattcgcaa gttcctaaaa tatattgcat aggcattttt gattttcttt
     7741 tttctaataa atttaagaaa tgtatttcat ctgacttttt aacttcttca tttttattcg
     7801 ttataagcca tattttatct ttttctaaaa tttttcctaa taataattga gtatctaata
     7861 tatatgtatc tatacctact tcttttaaag tttcatttcc taaatttaat aattctccaa
     7921 cttttttatt tggttctttc aacccttcag ccctccttaa agaagctatt gctacttcaa
     7981 tttgtaaatc atcaggctcc cttgtagtca atttttgcaa ttgtaatccc ggcgctgcta
     8041 ttatctttgc aaaatttcct tgagatttgc caagccattt tataagttca taagttattc
     8101 ctgatattac cggtaacatc gctactctta tagccgttct ctgagcaact gaatcccatt
     8161 tagtaaagga aaatataaat atacttacaa tagcaactaa aaatagaaag tttgttccac
     8221 atctaggatg aaatctagaa tattttctta cattttcaac tgtaagttca tcttcattct
     8281 cataacagaa tatagtctta tgttctgctc catgatattg aaatactctt tctatatcat
     8341 ctacttttcc cattaagtac atgtatccta aaagcatacc aatacttatg attccttcaa
     8401 tagcacttaa actccaatct ggaatattaa gttttttaag taaaaaagta attccagttg
     8461 gtattgctac aaataaaatt atggcaaata caaaggaaag cataattgta agtgtcatta
     8521 ttacattatt agctttttca ccaaatttat tatttaacca atcttcaaat ttagatggtt
     8581 ctgtatcatc aaaaaaactt gctgaataat ttaatgattt taatcctaca attaaagact
     8641 ctataagagt tacaaatcct cttataaaag gtagtcctaa aattttattt ttctttgtat
     8701 atggtatagt tttttcaaaa gaaacctcta tatctccctc tggagttcta actgctgtag
     8761 ctgttccttt tgatcccctc atcataacgc cttctaatac tgcttgaccg cctacttggc
     8821 attttctcat aatttgaacc ctatcctttc attaactact tatcagaatc cttaccatat
     8881 ttatatttaa aatagcattg tccacataga gattgatatt ctacattatc aactaaatct
     8941 atagccactt gttctccttc aaatgtgaac tttccattta tcattcttcc atttaatata
     9001 gcttttcttc cacatctaca tatagttttc atttcttcta tactatgtgc taataataaa
     9061 agtctttcac ttccttcaaa cccattcatt tggaagtctg ttcttaatcc gtaacaaatt
     9121 actggtattc ccttaccaac tgcaatttca aataattcat ctatgtgatg agcttttaaa
     9181 aattgaactt catcaactat tatacaatct atttttcttc ctttttcaag tctatcttca
     9241 attgtttcca taatattctc atcattatct attaaaatat ctacaactct ctcaacacct
     9301 agtcttgaaa caaccttatc tccacctttt ttatcagtag atggtttaat tattataact
     9361 tccatacctc tttcttcata attatgtgct acttgcataa gatgtgtaga tttacctgaa
     9421 ttcatagctc cgtatctaaa atataatttg ctcattttaa ctttaaactc cttatccttt
     9481 tataatattc taaatatgat tataacttaa ataagaaagc ttttaaacta ttgacgatta
     9541 aatatcccca tgatatactt gttaatagtt gtttagcaac atttttttat ttttgaaaga
     9601 ggtgaattac atgagacaag gcatacatcc agaataccat cacgatacag tggttaagtg
     9661 tgcatgtgga aacactttca caactggttc agttaaacca gaacttaaag ttgaaatatg
     9721 ctctaagtgc cacccattct tcacaggaaa acaaaaaatc gttgatgttg gcggaagagt
     9781 tgataaattc aacaagagat ttaatcttaa taaataattt ctttatggga aagactcaaa
     9841 tctttcccat ttttgtttat aaggcatatt tttcaaataa agtttgtttt tgtaaataga
     9901 tttttagtaa aagtgagtgg atatgatgaa aaaagttctt attttaagta catctactgg
     9961 ttatggtcat aatcaagcag ctaactcttt aatggaactt ataaagaatg atgatacaga
    10021 aattcttgta cacgattttc ttaaggagaa ccgtttcttt gatagatcaa tagtaaatgg
    10081 atacgattta tgtgcaagtt ccctaggaac tctttatggt ctactttaca aaattagtaa
    10141 tataaaattt ataaataatt tagttagttt tttatttttg ccagttgcaa ataaattagt
    10201 taaattcatt catagtttta atccagattt aataattaca acccatcctt tagccgtaag
    10261 tattctttct tatttaaaga aaagacaaat tataaaagta ccagttattt ctgttgttac
    10321 tgattttaaa tgtcattata cttatgtttc taaaataatt gatcattaca tagttgcttg
    10381 tgattttaca aaagaaaatc tagcctcaaa gggaatacct aaagaaagaa tatctccttt
    10441 tggtatacct gtaaagcaag atttttataa agaagattac cataattata tagagaatat
    10501 tatccaatca ccattaaata ttcttttaat gggaggcggt atggggttag ataatatatc
    10561 taaagtatta aaaaccctta tcaaaaatga taacccttta aatttaacta ttgtttgtgg
    10621 aaacaatgct gagttaaaga aagaattgtg taaagaatat gggcatataa cgggtaataa
    10681 aaatttaaat attttaggat acacaactga aatacctaag ataatgaaaa gttcggattt
    10741 aattattaca aaacctggtg gactaactac tacagaatca cttttaagtc atttaccaat
    10801 gataattcca tttataattc caggtcaaga aagtgaaaat agagaatttt tatctaaaag
    10861 taattgtgca attactatca accacttgga agaattaaat aaggtaatta atgatttaaa
    10921 taaagataat aataaattaa ttaatatgag acaaagtatt ttagatgtat taagttctta
    10981 ttctccagaa gaaacaatta aactttgcac taagatgcta aatgattctt ataacaaaag
    11041 aagatattaa agtttataga ctttaatatc ttcttttgtt ttgaaaactt atttaatttt
    11101 ctaaattgct ttttaaaaca acttgtaaaa actctttatt attttttgtt tttgataata
    11161 aatttataag cttttcagct acatcctcta tattattttc cttttgtaat actcttctta
    11221 ttttatatga tgcttctctt tcttcttcat taaataataa atcttctttt ctagtaccag
    11281 atttataaat atctatagct gggaatattc ttctctcttg aagctttcta tctaagtgta
    11341 cttccatatt tccagtaccc ttaaactctt caaaaatcat atcatccatt ctacttcctg
    11401 tgtcaactaa agaagttgct aaaattgtta aacttccgcc ctcttctaaa tttctagctg
    11461 ctccaaagaa tttcttaggc attataagtg cccctggatc taatcctcct gataaagttc
    11521 ttccagatgg agtaactgtt aagttatagg ctcttgataa tcttgttaaa gaatccatta
    11581 atataactac atctcttcct tgttcaacca ttcttttagc tctttctaat actatacttg
    11641 aaactttagc atggttttgt ggttcttcat caaaagttga atagattact tctccatcta
    11701 tacttctctt catatccgtt acttcttctg gtctttcatc tattaaaagt actataagtt
    11761 taacttcagg attattcttt gatatgtttt gagctattct ttttaaaaga gttgtttttc
    11821 cagccttagg tggtgcaact atcatccccc tttgtccctt acctattgga caaattatat
    11881 ccataagtct tgaacttaaa tctcttccat tctcagtctc aagtcttaat ctctcttttg
    11941 gatatattgg tgttaactct tcaaacttct ttcttccaac tgctttttca ggactttcac
    12001 catttactct ctctacataa agtaaagctt taaacttttc tccatccttt ggtattctaa
    12061 ctttaccttg gacttcgtcc ccagttctta atccaaatct tcttatttgt gatggagaaa
    12121 catatatatc atcttcactt gttaaataat ttctacatct taaaaagcca aaattattat
    12181 tatctaatat ttcaagtatt cccttagcag agtctgatga acttatcatg tcctttaact
    12241 gctctttttt tccttcatca tttgaattat tataattact attattgtta ttataattat
    12301 tactatttct aactaccctt tcaccagaaa aactttcttt gtttccacct tgctcttttt
    12361 gagatatttt ttctcttaaa ataacaccgt ctttatgtat tactttagta ttgctatttt
    12421 gtttattgaa ttcagctttt aattcctcaa ttaattcgct ttttttcttt ttacttatat
    12481 tttttattcc taattcttta gcctgctctt ttagctgaac taaagtcata ttttcatatt
    12541 gttctatatt caatttaagc acctcctgct tttttaatta gtggaataag gtaaagaaaa
    12601 gttattttag attgccttaa gacttttata tatagaaact tttgaatatc ctatttaaaa
    12661 atgtataaat ttcttatgtt aaatatttat attattatca tatcacataa aatctaatct
    12721 atatatagta tatttttata agattttatt tctaatatta ttaaataaaa aaggacacct
    12781 aacatttaag ttagatatcc ttaatatttc taaaattatt tattgtttaa tgcagcacca
    12841 acaaatccta cgaataatgg atgaggtctg tttggtcttg attttaactc tggatgatat
    12901 tgaactgcta cgaaccaagg atgatcctta acttctacac actcaactaa tcttccatct
    12961 ggtgaagtac cagctatagt taatccactt tctattaatt gagttctgta ttcattgttg
    13021 aactcatatc tatgtctatg tctttcgtag attaaatctg aagcataaac atccttacag
    13081 aatgtattat cttctaattt acatggatat aatcctaatc tcattgttcc acctaaatcc
    13141 tcaacatctt tttgctcagg cattaagtcg attactggga atggagtttc tggatttaac
    13201 tctgatgaat gagctccttc taatcctaat acatttctag cgtattctat aacagcacat
    13261 tgcattccta agcatattcc taagaatggt ttcttgtttt ctctagccca tcttatagct
    13321 tcgatttttc cttctactcc tctgtctccg aatccacctg gaactagaac tccatctact
    13381 ccacttaata attcatcaac attttcttta gttacatctt cagcatttat ccactctatt
    13441 tcaacattag tatcattgct taatccacca tgatttaaag cttcaactac tgatatgtat
    13501 gcatcatgta attcaacata tttaccaact aaagcaatct tagttgtatg agttaagtga
    13561 gttattctat gaaccatgtc tatccattca gcattatcta tatctctgca tccaagacct
    13621 aatttttcac aaactaatct gtctaatcct tctttgtgta acattagagg aacagcatat
    13681 aagtgctctg catctaagtt ttggataact tcacttgcat ctatattaca gaataatccg
    13741 atcttctttt tgatatcttc tgataattct ttttctgatc tacaaactat tatgtctggt
    13801 tgaattccta tacttcttaa ctctttaact gagtgttgag ttggttttgt ttttaactca
    13861 ccagcttttc ctaagtaagg tactaatgta acatgtatga aacaaacatt ttctttacct
    13921 acatcatatt tgatttgtct tatagcttct aagaaaggta atgattcgat atcacctatt
    13981 gttccaccga tctcagtgat tactacatct acatctcttt cttttcctac tctatgtact
    14041 ctatctttta tagcattagt tatgtgaggt ataacttgta cagtacctcc taagtattca
    14101 ccttttctct cttttgatat tactgaccag taaactctac ctgaagtaac atttgagttt
    14161 tgagttaagt tttcatctat gaatctttca tagtgaccta agtctaagtc tgtttcagca
    14221 ccatcatcag ttacgaaaac ttctccatgt tggtatgggc tcattgtacc tgggtcaatg
    14281 tttaggtatg gatcaaattt ttgaatgctt acacttaatc ctctgttttt taataatctt
    14341 cctaatgaag cagctgttat tccttttcct aatgaagaaa caactccacc tgttacaaat
    14401 acatacttag tatttttgct catatctctt cctcctaatg taaatttatt cttgacatcg
    14461 ttctttattt catttataat gataattacc tcatttagaa tgggtttttt attgcttgtt
    14521 ttaataacat agtagatatt ctatcaaaag ttaatgtaga agtctagtac ttttttgtgt
    14581 tttttaaatt ttttatttat agaacgttta ctatttatat tttgctcata tttaataaaa
    14641 ttaattatta atatttggat ttataagttg gaattagttt caactttttt tattttaaaa
    14701 ataaataatt tataaaaata aggctgtacc aaaaggtata gccttattta tctatttata
    14761 tactaatctt ctacttttat tttcatatca agatttgttt ttataggttc ttgtccacca
    14821 gctattagat gatattttat agagatattt cctccctttt catcaacatc taaatctaac
    14881 tcttttgttg atgtcttaag ttctaacatt ccataaggag tattataaag agctacttgt
    14941 gtattatttt tttgaaattc catttttgtg ctagttgttc cttctctatg taatacaact
    15001 ttatttccct ttatttttag agttgttttt gttccttcca ttcctgatat ctcagtttca
    15061 tcataagtag ctataaattc atctccatta atagaaaatt ctccaacagt tacaacttct
    15121 attttttctt tttctccaat atcttgagta ctacatattg atattatagc ttttttaatc
    15181 ataataatat cctttctaaa tcaattattg atttatcata tattttttaa gttcttcatc
    15241 tttaaatgga gttattgtta ttgtattgat ctctccattt ttctcataat ttatttcaac
    15301 agcgtcgcta tctttttctt tgcaatatct tccgcatatt ctagcagcaa tttctaaatc
    15361 ttctttatta aattctcctc ttaatataac aggagcccca gtagaatttt tagatgttac
    15421 taaaatatca tcttctcttt taaatttaac tatttctttc cactcttcac catttctagc
    15481 tactataact ttatttttag gtgatattct catatgtcta ccatacttaa gaacttcaat
    15541 ttcatcttta tctactgttt cttgattatc aagtaaatca cttaatctta ttgcatatcc
    15601 aggctctgtt aatttacatc cccctgctgg tgatggataa tcaactatat tccacttttc
    15661 agcaagctcc atttgagtct ttctacttct acctgatatt tttaacaatt tttctctatc
    15721 tactaagcca tttaactcca tctctgtagg ctctaagttt aaagcacata atggtcttaa
    15781 gattttttca ctaaatcctg attctttttt aacagtattt aatgcttgtc tattttgaga
    15841 cattggtctt tgatttaaaa cttcaccagt tattataaag tcagcgtcaa atttttttaa
    15901 taattctcct gaatatctca tcatcattgc atgacaatct atacatgggt tcatattttt
    15961 tcctctacca tgtttagggt ccttaaccat ttcaaaatgt tcttctgaaa aatctactac
    16021 ctctaaaggc atatctattt gtttacacat tctctttgca ttttcttcat taaagaaata
    16081 agatttaaaa catattccta ttacctctat tccttgatcc tttattaact ttgcagctaa
    16141 tatactatct aatccacctg aaatcatggc taatgctcta gtcatatttt tttcctccta
    16201 aaaatcactt tattatcata tgttaattct tttatttaaa taaatcaatt accttaaatt
    16261 caaaatttac gttattttcc tttttattaa cactcttaaa ttcttcttct gataatacat
    16321 ccttcatctt tttctctgtt tcatcatagg caacttgtcc cttagtaaca tctatgaaac
    16381 ataaaggtgg aaacataaca caccaccaat ttttaccttt tccttcgcca attagtattc
    16441 tataagcctc atattctcct tgtggcaaaa ctatattacc atattcttta acaggaaaat
    16501 tttctcttgt tagtgttgtt tcaacagtat aatcaaatcc ctgtgattta atataatctt
    16561 cagctatttt tataatgttt ttatcttctc tttttaatat ctctctagac tcttctaagc
    16621 tttgagattc atttaatata ggtgaaatat attttaacac ctcatcttta actctaagtt
    16681 ttaagtcttg atctatatca ctatcactat tggctaaaac gtgaaatctt attaactttt
    16741 cacttatatc ctcaacaata ttttcctctg taacacctaa agcttgtcta gtatttaaag
    16801 aacttcctac tataaataaa catataaatc ctaaaaataa aactgataat atttttttca
    16861 taacaaacac cccctgatac cttgattatt accaaggagt attttttata tacaataaaa
    16921 aatatttttt tataaattat tgctatttat agttcttgtt atatatacat ctgaatattt
    16981 atattttcct tttaaaaatt caaaagctct ttgagctgtt aacatatcat caaaaaaagc
    17041 aaatacagta ggtccacttc cactcatcat tgcacctaat gctccacttt ttctcatatc
    17101 ttcttttata ttttttaata tagtatgctt ctttaaagtt acattttcta aaacattttt
    17161 catatttttt gatacataat ctaaatcatc attttccata gcttgaatta agccttctat
    17221 attaacatgt tttcttatct tatttatatc taaatttgta taaacttctt tagtagatac
    17281 cccaaaatta ggttttaata caactaatat tttattttca aaaggtttta aaggggttat
    17341 aatctctcca attccttcac ataaagcagt tccaccttca atacaatatg gaacatctgc
    17401 tcctattttt aatcctattt cttttaacac ttctaaacta gcatttacat taaaaagctt
    17461 attcattata actaaaactg ccgccgcatt agtacttcca ccagccatcc ctgcagctac
    17521 aggtatattc ttcttaatac taatttttac accatttttt atattaaatt catctctaaa
    17581 taacactgcc gctttgtatc ctaagtttct ttcatctgta gggacataat gcttattaca
    17641 agaaatgctt atattactat cattactttt ctctatatct ataacgtcat acaaatctac
    17701 tgtttgcatt atcattctta ataaatgata gttatcttct ctttttccaa tagcatctaa
    17761 ggctatattt atttttgcat aagccttcat tttcatataa tcacccttct tacattaatt
    17821 aaagctaaat ataattatat tattttcttc taaaaacata aagagttaat taaaataaag
    17881 aaaaatcttc taaattacct actagaagat ttttctttta aattattttt actatatgct
    17941 agcacttttt aaatcttttg tgcccttatt tttaaattca tttattatat catatagaga
    18001 ttcataatct tcaaaaaaca ttataatagt ttcacctttt ttagctaact ttaaagtttc
    18061 tctaagtgct tcttcctctg acaatatgat ttttacattt aaatcttttc tatttgatga
    18121 taatacacct tcgtttatta tactagcaac ctcaccttta tcttttcctc ttaaatcttt
    18181 atcctctttt attactatgg aatcaaaaaa gtctccactt attttaccaa cttctttcat
    18241 agtatttaaa tctctatctc caggaacccc tattattcca gttatatttt taaaaccaag
    18301 atttttaaga gagtctataa ccaccttata tccatcaata ttatgaccat aatctaatat
    18361 tacatttaca ccattaacat caaacatatt aaatcttcct ctgttgtctt cttcacaaga
    18421 tttaaacttt tttaatcctt tagatataat gcaataatca acctctattc caactaaagc
    18481 tgcacaagct gccatagcat tttctatatt atgctttagt ttaccattca tagtaaattt
    18541 catatcctct gcagaagcta ttttgtattc tttacctcta ttttttacat atatagtttc
    18601 atccctataa aacacaatag gattttccag ttttaaattt tcctgtagat atttattatt
    18661 ttcattcatt gaaaataata ttttaggctt tttaattcta tctaaaactt ttaagctcca
    18721 ctcatcatca gcatttataa ctgaatatcc attatctttt acagcttcac caactaaaga
    18781 ttttacaaat gataaatctt ccatatcatt tattccatct atccccaaat gatcctctct
    18841 tatgttagtt attactccta catctgctaa atcataagct aatcctcttc ttaccaagcc
    18901 acctcttgca ctttctaaaa ctgctatatc aacctctgga tttaataata cagaccttgc
    18961 actattatat cctgtatcat cccccttatg tatatactta tttcctattt taactccaga
    19021 agtcgttgta caccctacat taaatccaat taaatttaaa acataagata ttaatcttac
    19081 tgtagtagtt tttccattag ttcctgtaac tgaaataaca ggaatattag aataattatt
    19141 tttaaacata ttatctaaaa tttttcttcc tacatttctt tctttgccta tagtaggaaa
    19201 atgatgcatt cttattcctg gggcagcatt aacttctaat attattccat cattttcata
    19261 tagaggctct gatattgatt ttgcacaaat atctatacca catatatcta atccaatagt
    19321 tttagctgct ctttcacaaa tttcaatatt ttctttacat attaagtcag tatagtcctc
    19381 agcacttccg cctgtagaaa gatttgcatt gcctcttaag gtaacttttt cgccatactc
    19441 taaaactgaa tttaaagaat atcctagctt acttaacata tttattaatt cttcatctat
    19501 cttaactttt gtcaaaggtt tttcatgccc ttctcctctt agtggattag cattcatagc
    19561 ttctattaaa tctctaatat ttctaactcc atcacctgtt atatatggag gttttcttaa
    19621 agaaactgct gctaccttat aatccactaa cattactcta tagtcatttc caaccttata
    19681 ttcttccatc attatttctt tacattcttt tgtgatttca gcataggctc ttaaaagctc
    19741 tttttcacta tttatattta caaaaactcc attacccttg cttccccatt gaggttttaa
    19801 tactacagga tacccaattc tattaaccac ttcaagaaga tgtattgtgt taaatatctt
    19861 ttctcctctg gcaacaggca agttttgtat atctaaaagt tcttttgtaa gaattttatc
    19921 acaggctata tctatagcaa ttccctttgt attattacca atagtagcac taaacctctt
    19981 tccttttttt ccatagccta tttggtataa attcccgttc cctactgtaa ttataggaag
    20041 gccaacttct ttagccgcat cacatatagc ctttgttgat ggccctatgc tctcttccat
    20101 aataactctc tttattattt ctattctttc atcaaagtta atatcttttt tctttattat
    20161 tgaattgatt aagtctatgg ctaattcagc tattaatatt gctgtttttt ttaatttata
    20221 ttcaaatatt atgtaataaa aatcgtcctt tatttctctt gcttttccgt attttatatc
    20281 tatacctagt ttattatgta ttcctataat tgtatgttca caaacatgag ctaagtatgt
    20341 tccctctttt aaacgttgaa caaatccgcc ttctatatct attccacatc tatgaacata
    20401 taactctgga acataactta ataacctttc attgaatcca tcaatatctt tagtaggagt
    20461 ttcacaatat ccctctaaat caacatctaa tctaatacat tttctatgtg aatatatgtt
    20521 tcttccctca aagatacgtt gattatttat cttcattttt atttctttcc tcctcaaaag
    20581 gcaccctagt tgataaattc atttttttgc ctgctgttaa tacatgtaac tttatattaa
    20641 atatactaaa tacctcatct tgtcttttct cagatacatt tgtatattca atatttcttc
    20701 catctacaaa ataaactgct ccactaccaa ttacagtcaa ttcatcatca ttaattacta
    20761 tagcagtgtc ttcatctata ccaattccta aaacttcagg attttggcac actcctgata
    20821 ataatctacc tatccttcct ctttgagcaa agtgttgatc tataagagta tttttaatta
    20881 aacctaatcc tgcagacata tttagtgaac tctttttagg tgattcccct tcttctccac
    20941 caactatcat aacatcactc atgacagaag cccctgctga tgttcctaca aaataacatc
    21001 cttcattact tcttttttgt aatgtatcat aaagaggagt tcccccaatt aaactagtta
    21061 ttctaagctg atctcctcca gtaaaaaata ttactgaagc ctcatttact aaagaaatat
    21121 tattttcatc aaaagcttct tctctttcta taacacgaag ctctttgatt tctcttattc
    21181 ccatacttga aaatatattt ttatactgtt ctatgactat attaggtgat tctgtagcta
    21241 ttgttgcaat caacataatt ccttccgatt ctttaaattt atctgctaca aaatttaata
    21301 taatcttttc acccttttta tcttctgctc cacctattat tattaaattt tttttactac
    21361 tacctttcaa attattcacc tcaaatcatg catttcttat ttttgccata aaatttatat
    21421 ttattcataa aaaagagttt tacgacataa tatcgtaaaa ctcttttttt tatagaactg
    21481 ctcttcctgg aatcataatt ttatcgccaa catatataga atctgcatct tctatatcat
    21541 ttaatttaac taaagtatct attgttgtct tgtatttttt tgctaaatcc cacaaagtat
    21601 cacctttttg aactgtgtat atagtaatgg atgctttctt ttcaacagtg tcatcatttt
    21661 gttcaatgtc tttaacatat tctttatcaa aaacatctga acatttagca taaaaatata
    21721 ctactgcttt aaccgctaag atatttcctt ctatactaca ttgtaaatct tctaaattag
    21781 ccttagtatc aaaagtcata ccagactgag aatttggcat atctaaagca acttcaaaag
    21841 gaatttctcc attcacttta ttaagtccct tttcctcatc agttgtcttg tatattgatt
    21901 caacagataa attacctttt ataacaacct tttgatcaac aaagtagctt tccatatcta
    21961 ctattttgcc tttacaatca actgtgtgag tagcatctac tccatttttc cccaagtcta
    22021 tatttccttt tattactgat tcatttgaat tttcactaac agccatatta atcttacaaa
    22081 cttcctttaa aggcattata ttttgcgttg gtgaatataa atcttcaact atttctgcaa
    22141 attctaattt tgatattgaa acatttgcat caacagataa attaacatct attattctac
    22201 tttctcctaa atcattttga gaaatattaa cattatgacc ttttatttca aaatatccat
    22261 tgcaattcat atctgaattt acacctacaa cctcttcatc atttgataaa tataaatcct
    22321 cttctaaagc tacaacatct ctagaatcat aagctctata aagaatatta attttagcca
    22381 agcaagatgc ttgtacttta tcttctccca attttatctc ttgtctatga agcatatatc
    22441 cacattttat tatttttcct atttgaggtt tatccatacc tacatttata ggtaattctc
    22501 cattcattga acaaaccata tttgtaatat tcttctctat actttctggt tttcttttta
    22561 tttgtaaatc tcctaaacca tctagttctt tgacaacatc tattttttct tctttataaa
    22621 ctctgtactt ggttcttagt tttccttcta ctcttatctt tctttcattg attatgttcg
    22681 cacctatatg ttctaaatca cattccatat cacacatcat actatgttca gccccagcta
    22741 tatcaataaa gctagaattt ttttctttat aaataacgct atttatccct aaaccttctt
    22801 cttctctagc taagtacata atattacact caacttcata ttctaataat actctatcac
    22861 tttgaacttc tctgttaatt acatatacct tagtatcaac tgaaagtaca tcatgaacat
    22921 ctggttgagt atctggtatt aagtattctt ctctaaaagg tatatctaaa gagttttcac
    22981 ttaagacctt ctcaaaacca agactttctt taataatatc taaattaacc aaatttatcc
    23041 ctcctaaata atctaatact ataatatata tgtatgatag ttaatttatg ataattctta
    23101 ttattgatac tttatttatt tttttattta aaacaacatt taagaaacgt ttacatgaat
    23161 ttttaaaata aaattaaatt tttttatctt ttttcgacat ttttttatta aaagtgttga
    23221 cacactgaaa aatatgtcgt attataaaga tggttattga ccatataacc tctcataaat
    23281 tctcaatacc cttttatacc atagttgaaa gatggaaacc caccatcttt ctttttttta
    23341 taaataaaat aaaaaagtta gctaattaat tagctaactt tttttattta accaacaaaa
    23401 tctagttgaa ccgtttttgt aagtacatct gaatagctgt atgtcacttt cctttgggtg
    23461 tctttttgta atctaactac aaaaatactt gggtgaacac tttctagtat tccttcattt
    23521 tccaaaatct tttttcttcc accattggct cttagcttaa ctttttcacc tacgtgatct
    23581 tctatgcttt ttctgatgga gttcaatgta agtctggtac tcattttaac accctctttc
    23641 tattatacat tatacactaa aagggatgtt ttttcaataa ataaataatt ttactatata
    23701 agtccatgtc ttgtcaacaa aaatatacta tatttaaact aaaacatcca attatattat
    23761 taacttaaat atcttatttt attctattaa tttaaatata aggattttgg ataaccttct
    23821 ctatattttc ctctagtttg taatccaccc actcccttta ttccagttat tgtattttct
    23881 ataacatctt ttattgaaac aatttcatct atccttgtat atgcaactct ttcacatact
    23941 aaaactggat ctaagcaatg tataagtact ctattaggtg aactcgcaaa attagcacct
    24001 gcttctaaaa tactttcata acaagattgg caagcaccag caaaaataac aagctcatca
    24061 taacttgaat tataatttct taaaattttt acagattcaa taaaatactt tgagtttcta
    24121 taattattta aatcaagcat tcccttggaa tctttaagaa ctccatcatg tccagttaat
    24181 acaactatat ctggattata ttccttaact aaagataaaa tttttaaagg ttgttctctt
    24241 tcggatactg ctattcctac agcatttaag tttaattgct tgtacgcctt taagcaggtt
    24301 tccatatatg atggatctcc atctatatga agtattctcc caggtcttcc aaataccaac
    24361 cctttttctg tcctttttaa attatcatct tcacttcctc ttgtcattaa ttgattattt
    24421 ttttgttctc tacttaaaat tactttttta ataatatcat tcattttaga atcaaagtat
    24481 ttatctttat ctccagcaaa ggcatcctca actttttcta aatcctctcc cttagagtca
    24541 gctattatcc ttatatgaat cccttttaaa atataagtta ttccttcctc attttcttta
    24601 atatctataa ttttaaaagt tatatcttta ctatatgatt ttcttactac taaatctcca
    24661 actttcatag attttctcct aacttatata gtcttattat cataatatga agttttattt
    24721 gtaagtgtgc tgatactata tactcatgta atttattgtt tttttattta aatttaaaaa
    24781 tatatattaa aaatccaata aaattaatat taaattagct ataaaacaaa ataaaaaatg
    24841 gtgcccaatt agtaggcacc taaaatataa ttatctaagc ttgattatat ttgcttttaa
    24901 attcttcaat aaatttcata taactttctt tctcattaat tatgttgttt attttattta
    24961 taaagatatt ttgtccccac ataacttcat tatagtagta tctatttgca agtctccaat
    25021 atctttgtgg gaataacaag tatgcatata atacctcata ttcttcttcc cttaatggac
    25081 ttactgtatt ataagcattt attatggcct ctgcatattc aatattccag tcaactcttt
    25141 ttaaaacctt cttcatgaag ttagctatgt catatgttct tatttctctt ttacaataat
    25201 caaagtctat tatatatact tcattatcat ctccaattat tatattgtga taagtataat
    25261 catgatgaca aaatactttc tcagcctctg cttcttcaca taaacttaaa tactttgaat
    25321 tatttaatat ttttgtggct cttattgcta actccttata aaaatcaaca ttttttatat
    25381 aaataatatc aaaatctttt tttaaatttt tctttctagc cattcctctc attttttcta
    25441 aggctttgcc tctcttttcc ataagatatg gccatcttcc taaatcactt tttaattttg
    25501 agttttctgg tggatcatat cccttgctag cttcatgtaa ttcagctaac tttttagcag
    25561 ctaatttaac ctcttctatg ttagtgaaat cacactctct tccctttatc caatttgaaa
    25621 gagtatataa atcctcattt actagagcat aaggctcatc ttcaatattt aagaaatatc
    25681 tatctatatg ttcaaatcca tttttagcta aatgctcctt tgctccataa acgaaaagaa
    25741 gtttttgagt tccatagttt atccttttta aacatctgtc acccttatta ctttttaaat
    25801 aatatacacc cttgctaggt tttaattttt ctattttaat atcaaattgt ctttctattt
    25861 caaactctct catcatatct attcaccccc taccatattt atatgtaaca acctcttatt
    25921 ttattaacat tttattccat atataaatat aataatatat atttatactg aaaggacata
    25981 tgctatgaaa atatgtattg atggaagagc agctacctta taccgaggta ctggaattgg
    26041 taactacact tatcaaataa taaataatct acaccagata gattttttaa atgaatataa
    26101 catacttact ccagaagcat cctctctaaa attacctaaa aagaataact ttaattattt
    26161 atcctcaagt acaaatgata aaaaaaactt ttgggaattt ataaatacaa aaaatcctaa
    26221 agaaaatatt ataggtgatg tatatcatat tcctcaaaat ggtataggct tttcaaaacc
    26281 taatgatata aagaccgtaa ttaccctaca tgatattatc cctatgaaaa tgcctgatac
    26341 agttagtgaa acattcttaa aaatttttaa tgaaaatata caaaatattt tagataacac
    26401 agatggaatt attacagttt ctaatttttc taaagaagat ataagtaaaa ctttttctta
    26461 cccaaaggaa aaaatatttg ttactcattt ggctgctgaa gaaatatata ccccattaaa
    26521 taaatttcac tcatctcaat atctaaaaaa acactatggt atagataggg attttttatt
    26581 atatgtaggt ggttttagcc ctagaaaaaa tattttagga cttatagatg cctttaactt
    26641 agtaaaaaat tcttataaaa gagatttaaa gcttgttatt ataggaacta agggaccttc
    26701 atatgaaatt tacagaaaaa aagtagatga gttaaattta tcttcctctg ttatttttac
    26761 tggatttatt cctatagatg atatgcctat attctatagt gctagtaaag ctttagttta
    26821 tccttctttt tatgaaggat ttggactacc tcctatagaa tgcatggcct gcggtactcc
    26881 tgtaatagca tctaatttaa cctcaatgcc tgaggtatgc caagatgctg ctcttttagt
    26941 tgatccttat gatgttgatg aaataaagga aaatatacta actttattaa ataaccataa
    27001 attttattcc cttatgattt ataaagggct aagtcattca agtaaattta attggaaaaa
    27061 aactgcttat aatacacttg aggtctacaa acatatttat tcacaaattt aaaatatact
    27121 ctctaaaatt tagttttaat atgtcttata aaaaatttat tattttaaaa tgaaaaggta
    27181 ctgtataaaa aatttaaaca taaaacttat aactttaata gaatttctta cttctaagaa
//
LOCUS       DAEVGI010000020        25525 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEVGI010000020 DAEVGI010000000
VERSION     DAEVGI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 25525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25525
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.020"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(32..1030)
                     /locus_tag="K8M77_000791"
     CDS             complement(32..1030)
                     /locus_tag="K8M77_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBI7085306.1"
                     /translation="MRSLNGNLIRISGELLKITLNKEVTGTLYYSEKLNGELKFIKHI
                     EKENEFEFNDPEPKNRTFYFIKSENEELILAERLVPLKRFCNFRDLGGYETKDGRKVK
                     WGLFYRSEALNKLKGEDLEYFKTLGIKYIFDYRSLEEVKLSPDRNVEGAKNINISAMR
                     NLDNQNLNMESYLKGILLKDSNQELPEKILMDGYSEMPLNNLAFKELINIIENPKNLA
                     VLQHCTSGKDRTGLGSALILLLLGVSEEKVIEDYMLSNEYRKGENHRILQIYEKHVSN
                     ETIKELIEGILGVKKKFIELSLNKIKDTYGSYETYFLKEYGLTKEKIEFLRDEYLY"
     gene            complement(1551..1916)
                     /locus_tag="K8M77_000792"
     CDS             complement(1551..1916)
                     /locus_tag="K8M77_000792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085307.1"
                     /translation="MEYIQLIAIIFCIYIVISILEQITLIEDFYSLLKTGSEIKQDDL
                     REFMQETKERFKKENICYLIMLHFINIICIVITVFIVTFGDVRGCFIPIIVGIFLLGF
                     ILTIVLNVEGVFKNMDINL"
     gene            complement(2209..2643)
                     /locus_tag="K8M77_000793"
     CDS             complement(2209..2643)
                     /locus_tag="K8M77_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin resistance protein BacA"
                     /protein_id="HBI7085308.1"
                     /translation="MLDQKTIDIIKSTVPVLKSNGLKITKTFYKNMFEQNPEVKPLFN
                     MNKQESEEQPKALAMAILAVAQNIDNLEAIKPVVNRIGVIHCNTQVQPEHYPIVGKHL
                     LGAIKEVLGDDATEDIINAWAKTYEVIAEVFINNEKEMYASR"
     gene            complement(3068..3805)
                     /locus_tag="K8M77_000794"
     CDS             complement(3068..3805)
                     /locus_tag="K8M77_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7085309.1"
                     /translation="MSGHSKWHNIQAKKGKMDAKRGKIFTKIGKEIAVAVKEGGANLD
                     GNSRLKDAVAKAKAANMPNDNIQRAIKKAAGEGDSVNYESIVYEGYGPSGVAVMVEVL
                     TDNKNRSAGNVRSAFTKGGGNMGTSGCVGFMFQKKGEIVIEKAELDEDEIMMMALDAG
                     AEDFASEEEVFIVTTSPEDFGTVREALEAEGLEFLEAAVKMIPDTETAINEDDAKKFQ
                     KMLDLLEDDDDVQEVYHNAEFPEGWDE"
     gene            complement(3931..4584)
                     /locus_tag="K8M77_000795"
     CDS             complement(3931..4584)
                     /locus_tag="K8M77_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBI7085310.1"
                     /translation="MNYITVKKAAEDRFEEKKSVFIGYVKRVTTEEEAKEFVAEINNK
                     HKDARHNCYAYVIGQNMGIQRYSDNGEPQGTAGIPILEVIKKQGITDCAVVVTRYFGG
                     ILLGTGGLTRAYTKGAAIAISAAGIVEKVEGAMLCTEIDYDLYGKIQYICGQNNWHIE
                     DTEFTDSVKIYILAEKTMIDEIKKSFTEATNGKAIMSTDLEGVYFKEGNRLYESFDE"
     gene            complement(4590..5996)
                     /locus_tag="K8M77_000796"
     CDS             complement(4590..5996)
                     /locus_tag="K8M77_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBI7085311.1"
                     /translation="MKKFSVVFKEDLCKYLIIYDNIKSLIENGEISEGEKLPTIRELA
                     DFLEVNKVTVINAYKKLVQEGYAYQSQGSGTYAKNKEVGKSFKHDYNDLFRKIASGDL
                     KNWIDFTGETTSANFFQVEKLKKVLDNVLVRDGVEALMYSDPLGYLELRRSINEEFWN
                     GKNNLDNILIISGAQQGIDIVGKSLVNINDNIVIERPTYGGALLVFKLRRANILEIPM
                     EKDGPNIEKFENLLKRNKIKCFYTMSYFQNPTGISCSLGKKKKIIELAHKYDFYILED
                     DYLSELVYSNDLEYIPYRSLDSERVIYIKSFSKIFLPGIRMGYLIAPDKFKEEFQALK
                     FNSDIATSSLMQRALQDYIAKGYWKEHIERLNEEYSKRYNFIKELIDNKLGDMVSYRE
                     PKGGLNLFLNINKNIDITSKKLFYELKDRQTIITPGSIFFKNPNDGDKSFRIGFSQID
                     YSKIEKGIDNIYDVLKGR"
     gene            complement(6011..6913)
                     /locus_tag="K8M77_000797"
     CDS             complement(6011..6913)
                     /locus_tag="K8M77_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBI7085312.1"
                     /translation="MSEIILKYQNSFKTVINDLQKNEDVIGVTAFGSIINGDIWEGSD
                     IDLFVIVNGDFKGVKDVYGEELGVSLHLKIVSKKFLVNFCQEEITGSTIHRKFISSKL
                     IFCKDQEISDKFTLIKYYTDAHSEKWNLVYLSELLQDMNLYKKCIHNEHFYAAFEALM
                     YSVDSFSRLYLNFNGYLITKSSTGMLMKLDENFEKMVNGIFKASDKQALEYVHFYIES
                     FLERNIRGITRALADFMKKQKDYLSSEEIKKSEEFQGFKIDFEELLGYLYKKNIVKKN
                     GREKFSPCGQKLITEKVYLIKDNV"
     gene            complement(6955..7425)
                     /locus_tag="K8M77_000798"
                     /pseudo
     CDS             complement(6955..7425)
                     /locus_tag="K8M77_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7557..9350)
                     /gene="hflX"
                     /locus_tag="K8M77_000799"
     CDS             complement(7557..9350)
                     /gene="hflX"
                     /locus_tag="K8M77_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBI7085313.1"
                     /translation="MIHGNTDGIRNSVLKELEELYKINVPKYSVCTEDMINIISRLSS
                     QIEREISVGINRKGKVISVAIGDSSSVEIPLIDIHEKRLSGVRIIHTHPSGVSRLSAL
                     DISALMKLKLDTIMAIAVEEGKAKDASLGFCYVENEILISEEAPHLTIDKCLQINILD
                     KINHVEEVIKTSNVEEDDSERAILVGCDTRESLDELEELAKACDIPTLEKVFQNRSKI
                     DASFYIGRGKVLEIANIRQLTRANLVIFDDELSGAQVKNLEANLGVKVIDRTTLILEI
                     FSRRAKTREAKIQVELAQLKYRASRLMGLGTVMSRTGGGIGTRGPGEKKLEIDRRHIR
                     ERIYDLQAELKKIKKIRETQRERRSKDKTSQVSLVGYTNAGKSTLRNTLCAESASTLA
                     TQAKDKVFEADMLFATLDTTTRAIKLPDNRDITLTDTVGFVSKLPHELVEAFKSTLEE
                     VIYSDLLCHVVDASSDNAQEEIIAVEKVLGELKALESAKILVLNKIDKADEEKLNELE
                     AKYSSIYNKVVKISARERINLDNLLEAISEELPYTLKSKEYIIPYTSQQVVAYLHRNT
                     NVSEEEYREDGTYIKAEVDEEVENKCREYEL"
     gene            9457..9981
                     /gene="hpt"
                     /locus_tag="K8M77_000800"
     CDS             9457..9981
                     /gene="hpt"
                     /locus_tag="K8M77_000800"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBI7085314.1"
                     /translation="MEDKNRNLLISENQIKEQIAIVGKKITEDYKDKNLYVLSLLRGS
                     FIFTADLVRTIDTKTQIGFMTTSSYGHGEESTGIVKVVNDIPDNIEGLDVLIVDDIID
                     SGITMDFVINHVKSLGAKSVKTCTLLDKPSRRKVDLKPDYCCFEIEDLFVVGYGLNYG
                     DHYRNIPYIFNWEK"
     gene            complement(10015..10704)
                     /gene="yunB"
                     /locus_tag="K8M77_000801"
     CDS             complement(10015..10704)
                     /gene="yunB"
                     /locus_tag="K8M77_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBI7085315.1"
                     /translation="MYYVNKNKSKIIDFKKYLIIILLMIIACFLIFIYAFDKIIAPTV
                     LLVSDSEMQAKTLDIINTNIAEVYTEKFNYNDVIKVQKDSYGAIKMINADTIKLNELA
                     TEVAIKSQRDIKEVGSIGVKMPIGYITKNNIMSYWGPSITVKMEPIGRVVVTYESLFE
                     SAGINQTRHKILVNVKTNLKIILPLKSKEVEVVNQIPISDTVIVGEVPNSMWGGNMLQ
                     GINEESREDTN"
     gene            10841..13303
                     /locus_tag="K8M77_000802"
     CDS             10841..13303
                     /locus_tag="K8M77_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBI7085316.1"
                     /translation="MTERKREYKDRKQKKNSPKNQSKVTKFLKWFFIGVLLLGITAIT
                     IVGFYVLSIIRSSPELDVQAIQSLNQPSILYDDQGNFMDNVITREQRYVVKSDEIPDN
                     LKKAFVAIEDERFYEHKGIDIKRIAGVIASNIKGKLSGSNTVQGASTITQQLIKNAVL
                     TNEVSYERKIKEMYLALELEKHLSKDEILTTYLNTIPMGGYQYGVSAAAQRFFSKNVS
                     DLNLIECAYLGGLTQAPTSYDGLSEINKENPSRYLNRTKSVLFKMHELGYISSEQYNN
                     AINEIDTTGIKFKPNNKLSKTNFEWFTRPTITQVKQDLMNKYKYTQDEVDKLIANGGL
                     KIYTSMDRNLQNNVQKVLDDPNNYKAITNNPNEKNEDDVYKLQASATIIDYKTGHVKA
                     LVGGRGEQPAMSHNRAYYDLKSIGSATKPLTVYGPAIDLGLGGAGSVVNDSPLTNKEL
                     SSTGYKDQPKNEYNSYRGPLTFREAIKISSNLAAIKVANEVGVSNSIAYGEKLGLVYG
                     PHSRGISTTALGQFQNDPNNPDGGNTYTLASAFGVFGNNGVKTNAKLYTKVLDSHGNV
                     LLDTSTPEEIKIFSPQTSYIVYDMLKDQVESGSAKPAKFGNIPVAGKTGTTTGDKDYL
                     FAGLTPYYSAAIWIGYDKPREMRTSSGIVTSPIFGKIMGLAHKGLQYKEVEQPSGISK
                     IAVCMDSGLKPTSLCTQDPRGSRVYYDWFINGSAPNQYCNYHTNSHVNNFNKNNNTTG
                     VNEKNKKQETKTEEIKPNENNKSNEQTPNTNSDNAPNNVPNNDNNNSNNIGGNMIPPQ
                     NQTENNTNNGVITPNQSINNKN"
     gene            13626..15026
                     /locus_tag="K8M77_000803"
     CDS             13626..15026
                     /locus_tag="K8M77_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBI7085317.1"
                     /translation="MEILNNLIVSFNDFLWGYILIILLICAGLLFSFKTKFVQFRYTK
                     EMFRLLGDGIGKSNKEKGGVSSFQAFCISTASRVGTGNIAGVAIAVAVGGPGAVFWMW
                     LIALIGSASSFVESTLAQIYKTKDKDSFRGGPAYYMEKGLGKRWLGIIFSILITISFG
                     FVFNAVQANTVSVAFNNAFGLSREAIGIILVIVTALVIFGGIHRVAKVSEIIVPILAV
                     LYILIAIIVLILNITEIPSVFKLIFESAFGIKQLTAGGLGAAVMQGIKRGLFSNEAGM
                     GSAPNAAATADVTHPVKQGLIQTLGVFTDTLLICSCTAFIILLSGQYADSSLTGIELT
                     QKALVSQIGPFGAYFIAICILLFAFSSIVGNFYYGQTNIEFIHTNKILLNIYRILVIG
                     MVLFGSIAKVDLVWNLADLFMGLMAILNIITILFLGKYSFLALKDYTAQKKAGIKEPV
                     FKASSIPGLENVEEWQ"
     gene            complement(15061..15453)
                     /locus_tag="K8M77_000804"
     CDS             complement(15061..15453)
                     /locus_tag="K8M77_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085318.1"
                     /translation="MINIKLDEDKRGKVIFRLNIDECHKENRILKRALFESRVVKNEF
                     KYNIPMKYFWPIINNVHKELISLSEDSRLEVLEFSDEYEEVYYYNYKATPAYMKKWRE
                     EGCPPIFKITINPKDLSVEKKIIFERLI"
     gene            complement(15501..16577)
                     /locus_tag="K8M77_000805"
     CDS             complement(15501..16577)
                     /locus_tag="K8M77_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085319.1"
                     /translation="MIKKRNCYRREGRKVYIRTPYLEELSYTEKLWGDKMSMTDIGKT
                     ISFPRYKWDSFYKKMVSPTDGRNLYCLVFDYNDNPVGEASFHGYDSITKTARLNVRIE
                     SEHRGKGYGSEAVYLILEYFFNEFGGLYMLDTVSLDENRERLEKIGFKIMYKNKDGYS
                     YRLSKEDFNSQIKDLKERKFGIFIYDKMVVSTVISFLEIFHDINKSLDKEIISVELVG
                     AKNNIIKDGMYDIKFENVKSIEEVDLDVIIVPGSKSNKFYENEEFEKLLNNINGCEIV
                     FIIGNSIIPFIKNKFLRGLKTYYSKEWVELIKEELALISYSDEDIIDNGRLIMVKKKS
                     CEIEGIKYIIRKLFGESLLNRFDL"
     gene            16900..17439
                     /locus_tag="K8M77_000806"
     CDS             16900..17439
                     /locus_tag="K8M77_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085320.1"
                     /translation="MEIGDKIRRLRVAKQLTQEELANRCELSKGFISQLENDLTSPSI
                     ATLIDILDILGTNLTEFFSEDTNEKIAFSKDDMFETENEELKYNLMWLVPNAQKNDME
                     PIMITIKPGGQYIEEEPHEGEEFGYVLSGAIYLHLGEKKVKVRKGESFYFKPKANHYI
                     SNAGKISAKVIWISTPPSF"
     gene            17471..18520
                     /locus_tag="K8M77_000807"
     CDS             17471..18520
                     /locus_tag="K8M77_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085321.1"
                     /translation="MKDNNIIELKGITKSYGKDTILDNLSLNIKKNEFLTLLGPSGCG
                     KTTTLKIIAGFETADSGQVVFENNIINDIPPYERQLNTVFQKYALFPHMNVYENIAFG
                     LKLKKIPKDIIDQKVKEMLKLVALEGYENRDIEALSGGQQQRVAIARALVNEPKVLLL
                     DEPLGALDMKLRKEMQIELKKIQQKLGITFIFVTHDQEEALTMSDTIVVMNKGEIQQM
                     GSPEDIYNEPANSFVAKFIGESNIVDGIMLDDFKVEFDGKIFDCVDKGFEKNEAIEVV
                     IRPEDFEMVKYENGMLKGTVTSIIFKGVHYEIEVKNENHTWILHNTKHAEIGSKIGLS
                     LDPESIHIMKKESDV"
     gene            18523..19368
                     /locus_tag="K8M77_000808"
     CDS             18523..19368
                     /locus_tag="K8M77_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085322.1"
                     /translation="MKNRNKNLSFAAYPYVLWSAIFIVIPLLLIVFFSFTSKVDGRFV
                     FSFENFQRLFEPIYFTVFIRSIWLAVLSTVLCLILGYPIAYMISKLPIKRRNMLILLF
                     ILPMWMNFLLRTYAWMAILGRDGLINTLLGYIGIGPIKMLYTDGAILLGMVYNFLPFM
                     VIPIYTVLIKIDKNLVNAAYDLGANKAQAFRKVILPLSIPGIISGITMVFMPAVSNFV
                     IPNLLGGGKYMLVGNLIEQQFTTIGNWNFGSALSIFMMILILISMAFMSKYEKNGKEG
                     GKQLW"
     gene            19362..20162
                     /locus_tag="K8M77_000809"
     CDS             19362..20162
                     /locus_tag="K8M77_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085323.1"
                     /translation="MVKKLGDFISKFYLILVFIFLYAPIVTLMIFSFNKTKSMAVWGG
                     FTFDWYSSLLENDRIISALYYTILIAVIASVIATVIGTISAIGINNMKKGPRTLLLNV
                     NYLPVLNPDIVTGISLMTLFAIGGVLFNLKPGFWTMLLAHITFDIPYVILSVLPKLRQ
                     LPPNLTDAALDLGAKPSYALRKVVIPQIMPGIAAGFLIAFTMSIDDFVISFFTTGPGV
                     SNLSIEIYSMARRGISPEINALSTLMFIVVLALLLIANRKQSLVKEER"
     gene            20164..21246
                     /locus_tag="K8M77_000810"
     CDS             20164..21246
                     /locus_tag="K8M77_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI7085324.1"
                     /translation="MKLKKFLALTCSTLLLSSLFLGCGPKKDEEATQDKNNNVLYVYN
                     WGDYIDPELLTKFKEETGIDVKYDVYDTNEIMYQKLNSGNVSYDIVIPSDYMIEKMKE
                     EDMLAKIDYSNIPNYKYIGEQFKNLAYDPTNEYSVPYMWGTVGIIYNTKKVSDPVDSW
                     NILWNPKYKDQVIMPDSVRDAMAVAEKKLGYSLNTENLDQIEAAKKELMTQKKDGLIL
                     AYMVDQVKDAMVGGEASLAVAWSGDAVTMIERNPDLAYAIPKEGSNKWFDAIGIPKNA
                     KHKENAEKFINFLCDSENAEQNVEYIGYSTPNTAAYDLLPEDIRDNKVAYPDKESLKN
                     CEVFIDLPSKILRKYDEAWLEIKCIY"
     gene            complement(21405..21836)
                     /gene="ndk"
                     /locus_tag="K8M77_000811"
     CDS             complement(21405..21836)
                     /gene="ndk"
                     /locus_tag="K8M77_000811"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBI7085325.1"
                     /translation="MRLEKSLVLIKPDAVERNLIGKILEVYEGAGLKIKAMEMKQINK
                     DFAEKHYEEHRDKQFFNSLIKYITRSPLVALILEGEDAIDKIRSLNGATNPEKAEFGT
                     IRRRFALSGTENSVHASDSIESAEKEIKLWFPKVFYEEICG"
     gene            22021..22290
                     /locus_tag="K8M77_000812"
     CDS             22021..22290
                     /locus_tag="K8M77_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBI7085326.1"
                     /translation="MIRKEFLVSGRVQGVGFRFFCKYQASLLSLTGYAENLDDGQVLI
                     EVQGDESSIRKFKTKILNGNGFSRVISIDEKDLTVDTREKRFSTY"
     gene            complement(22430..22585)
                     /locus_tag="K8M77_000813"
     CDS             complement(22430..22585)
                     /locus_tag="K8M77_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085327.1"
                     /translation="MKGNKNNKNYNNKKHKDHEPDDGTFISYKENEEFNSNGSSRYGY
                     EQDSCDL"
     gene            complement(22754..23320)
                     /locus_tag="K8M77_000814"
     CDS             complement(22754..23320)
                     /locus_tag="K8M77_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085328.1"
                     /translation="MAALTVDDILKESEVGTLIPLIVEVNDKVVPIIFVKDYLEALKD
                     IGDNVLVALKSSIISNKKLDLLLIMIRFDEREENTYDLWLNYGFNWHFDFLNQLINLD
                     KILIDFRDEDNNRIKTIEVCNTIKKDLEKYKESCEDSILIKEGKEENIIKVVKRKKYE
                     SWNNEDALNLIDEVLDDYSSIQDMWDNL"
     gene            23499..24770
                     /locus_tag="K8M77_000815"
     CDS             23499..24770
                     /locus_tag="K8M77_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBI7085329.1"
                     /translation="MIKFKKKLLSSILIALSVSLFAPIKATVQAAEISQPNIVGKYAV
                     TLDYETGEIIYAKGIDEKAYPASTTKVMTSLLFAEHASKNDSFPYTADAKVQQPYTLN
                     DSFGPIPVGEGMNANDLMKALLMFSANDAAAVIADGVAGSTEKFSVMMNDEVKKLGLK
                     NTHFVTPNGLHNDDHYSTAYDLAVILQNAYKNPWVRETMALKDSDITVNGKKVLLENR
                     NKELGINGNIGGKTGFTTPAGRCLVSVYERNGRKIIGTVLNSQYDAKDEIVFNDMNKI
                     IDYSYSVDKVPYIKAGTTIDTIPVEYKLFRWFGPTKKIDVPFVATENIDYYKNYVNEK
                     ETSKSINLNDMNAWQLASNPESAAVTVTQRAYVKDYPVKADIGTFTLIKANFLSYLGI
                     IVLVAIAIVLILLIIRAINLRKRKKRRRNIF"
     gene            complement(24827..25471)
                     /gene="ppaX"
                     /locus_tag="K8M77_000816"
     CDS             complement(24827..25471)
                     /gene="ppaX"
                     /locus_tag="K8M77_000816"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphatase PpaX"
                     /protein_id="HBI7085330.1"
                     /translation="MIKAVLFDLDGTLINTNDLILKSFKHTFKTILDLEPSEEEITMN
                     YGRPLQEIFKSYDENRIEEMINCYRKINLELHDDECKEFAGVDLMLKTLKSKGIKIGV
                     VTSKKNDMAERGAKLMGIFKYFDTFITSEITTKHKPDGEPVLKACENLGVSPSEALMV
                     GDSPYDILAGKNAGAKTCGVKYTALPLEKLEESKPDFYVDKPLEILDLVEKLNS"
BASE COUNT         8722 a         3972 c         3112 g         9719 t
ORIGIN      
        1 aaaggttgta agatttctta aatttattta actaatataa atattcatct cttaaaaact
       61 ctattttttc cttagttaat ccgtattctt ttaaaaaata tgtttcatag ctaccataag
      121 tatcttttat cttatttaaa ctaagttcta taaatttctt ttttactcct aaaatcccct
      181 ctattaattc ctttatggtt tcatttgata catgcttttc ataaatttga agtattctat
      241 gattttcccc ttttctatat tcattactta acatataatc ttctattacc ttttcttctg
      301 acacacctaa aagcaataat attaaggctg atcctaaacc agttctatct tttcctgagg
      361 tgcaatgctg taatacagct aggtttttag gattttctat tatatttata agttccttaa
      421 aagctaaatt atttaatggc atctcactat atccatccat aagtatcttt tcaggaagtt
      481 cttgattgct atcctttaat aaaattcctt ttaaatagga ttccatattt agattttgat
      541 tatctaaatt tctcattgca gatatattta tattttttgc cccctcaaca tttctatctg
      601 ggcttaattt aacttcttct aaggatctat agtcaaaaat atatttaatt cctagggttt
      661 taaaatattc taaatcttct ccttttaatt tgtttaaagc ttctgagcga taaaataatc
      721 cccacttaac ttttcttcca tcctttgttt catatccacc taaatctcta aaattacaaa
      781 atcttttaag tggcacaagt ctctctgcta aaatcaactc ttcattttca ctttttataa
      841 aataaaaagt tctattctta ggctctggat cattaaattc aaattcattt tctttttcta
      901 tatgtttaat aaactttaat tctccattaa gtttctcaga gtaatataaa gttccagtaa
      961 cttctttatt taatgttatt tttaacaatt ctccagagat tcttattaaa tttccattta
     1021 agcttctcat aaaacatcat ctcctaatat aacccaaatc ctttttttaa ttatactact
     1081 tatttttata aattgttaat ttttattaaa ccttatatat attttctctt tatcataaac
     1141 ttcttaactc tcttaagttt aaagaaattt tttcaaagaa ttaatgtttt tccatattat
     1201 gtatgataag atgttattat aagatgtaaa acttattatt tatatatttt tagatataat
     1261 aagatttatt tttctatagt aataaataat ttaaactttt tattatattt aaatctattt
     1321 tatttatgct taaggaggtc ccatggaatt attatttata ttgctttttt taataatgct
     1381 tataactgca ttttcaacct cactaagaga aactaagaaa taatttatta aaatttattt
     1441 tacattatat attctgacaa aataaaggca gtaatattta atatgtaaaa attaaacatt
     1501 aaatattact gcctttaaat aaatttattt tttaaaatct tactttctta ttataaattt
     1561 atatccatat ttttaaatac accttctaca tttagaacta ttgtcaatat aaatcctaat
     1621 aaaaatattc caactataat tggaataaaa caacctctta catctccaaa agtaacaata
     1681 aaaactgtta taactatgca aattatattt ataaaatgca acatgattaa ataacatata
     1741 ttttcttttt taaatctttc cttagtttct tgcataaact ctcttaaatc atcttgcttt
     1801 atttcactac cggtttttaa aagactataa aaatcttcta ttaaagttat ttgctctaat
     1861 attgatatta caatataaat acagaaaatt attgctatta attgtatgta ttccatatta
     1921 attcctcttt tctaaatata tatttatatt atatcaaatt tacatttagc tatatattgg
     1981 cttataactt taaaatatca aaaattttta caatatctta tttaatatat atttttaagt
     2041 aacaaaaggt tatttcctat ctataaagat ggaatataat ttgtatttaa tagaaaaact
     2101 caataagtat attgagaaaa tcacataagc aaaaataatt tccgtaaata atatctaaaa
     2161 atcaataaaa aagatgagct gatttaatag ctcatcttat atattgttct atcttgaagc
     2221 atacatctct ttttcattat ttataaacac ttcagctata acctcatatg ttttagccca
     2281 agcattgata atatcttcag ttgcatcatc acctaaaact tcttttattg ctcctaataa
     2341 gtgtttacct actattggat aatgttctgg ttgaacttgt gtattacagt gtataactcc
     2401 tattctatta actacaggtt ttatagcttc taagttatct atattttgag caactgctaa
     2461 tattgccatt gctaaagctt ttggttgctc ttcagattct tgtttattca tattgaacaa
     2521 aggttttact tcagggtttt gctcaaacat atttttatag aaagtttttg ttatttttaa
     2581 tccattagat tttaaaactg gtactgtact ttttattata tctattgtct tttgatctaa
     2641 cataatctcc tccataatat ttatttttaa ttaataatta ttattgatta tattatacaa
     2701 caatttaaaa ttttttcaag ttaaaaacat taaatatttt tttagtaaaa tgttaactta
     2761 taactaataa tacaaaatca taaattataa ccaatattta aatctgctat actattttat
     2821 taaacaaaca ctataattag tattatctat ataagtaaat ttaataaaat acaccctaat
     2881 ctataaacct aataatatac cgtaaaacta aatttttatt aaatttaaat aaaatttgaa
     2941 aatacaaaaa acaccctttc aatgtttata taaaattatt attttatatt aacattaaaa
     3001 gggtgttctc ttacttttat atatcattat ctattatttt ttgattttct aaaatgctta
     3061 aaacgtatta ttcatcccat ccttcaggga actcagcatt gtgatacact tcttgaacat
     3121 cgtcgtcatc ttcaagcaag tctaacattt tttggaactt cttagcatcg tcttcgttta
     3181 tagcagtttc agtatctggt atcattttaa ctgctgcttc taagaattct aatccttcag
     3241 cttctaaagc ttctcttact gtaccaaaat cttctggtga agttgtaact atgaatactt
     3301 cttcctctga tgcaaaatct tctgctccag catctaaagc catcatcatt atttcatctt
     3361 catctaattc agctttttct attactattt cacctttctt ttggaacatg aaaccaacac
     3421 atcctgatgt acccatgttt ccgccacctt tagtgaaagc acttcttacg ttaccagctg
     3481 atctattttt attatctgtt aatacttcaa ccattacagc tactcctgat ggtccgtatc
     3541 cttcataaac tattgattcg tagtttactg aatctccttc tccagcagct ttctttatag
     3601 ctctttgaat gttatcgttt ggcatgtttg cagccttagc ttttgctaca gcgtccttta
     3661 atcttgagtt accatctaag tttgctccac cttctttaac tgcaacagct atctcttttc
     3721 ctatcttagt gaatattttt cctcttttgg catccatttt acctttttta gcttgtatat
     3781 tatgccattt tgaatgtcct gacatcctta ttcctcctaa catatatgtg aactatagtt
     3841 taaatttctt taaatatctt gaaataatac ctaaatatta tatcatagag attttttctc
     3901 ttcaacttgg ctcttttcta tagttaaagc ttactcatca aaactttcat aaagtctatt
     3961 tccttcttta aaataaactc cttctaaatc agtactcatt atagcctttc catttgttgc
     4021 ttctgtaaag ctttttttta tttcatctat catagttttt tctgctaata tatatatctt
     4081 aacactatca gtaaactctg tatcctctat gtgccaatta ttttgaccgc atatatattg
     4141 aatctttcca taaagatcat aatctatttc tgtacaaagc attgctcctt ctactttttc
     4201 tacaatacct gctgcactta tagctatggc cgccccttta gtataagctc tagtaagtcc
     4261 ccctgtgcct aataatattc ctccaaaata tcttgttact actactgcac agtctgttat
     4321 tccttgtttt tttattacct ctaatattgg aatacctgct gttccctgtg gctcaccatt
     4381 atcactatat ctttgtattc ccatattttg tcctataaca taggcataac aattatgtct
     4441 tgcatcctta tgtttattat ttatttctgc tacaaactct ttagcctctt cctctgttgt
     4501 aactctttta acatagccta taaaaactga ttttttctct tcaaatctat cttcagctgc
     4561 tttttttacc gttatataat tcataatatc tacctacctt ttaatacatc gtatatatta
     4621 tctattcctt tttctatctt actataatct atttgggaaa atccaattct aaagctttta
     4681 tctccatcat taggattttt aaagaatatg cttcctggag taattatagt ttgtctatct
     4741 ttcagttcat aaaataactt tttagaagtt atgtctatgt tcttatttat gtttaagaat
     4801 aaattaagtc ctcccttagg ctctctataa gaaaccatat ctcctaattt attatctata
     4861 agttctttta taaaattata tctctttgaa tattcctcat ttaatctttc tatatgctct
     4921 ttccaatatc cctttgcaat ataatcctga agagctcttt gcattaaact tgaggtagct
     4981 atatcactat taaactttaa agcttgaaat tcctctttaa atttatctgg tgctattaaa
     5041 tatcccattc ttatacctgg tagaaatatt ttagaaaaac tttttatata tataactctt
     5101 tcagaatcta aactcctata tggtatatac tctaaatcat tagaataaac taattcggaa
     5161 agataatcat cttctaatat gtaaaaatca tatttatgtg caagttctat tatttttttc
     5221 ttttttccta aagaacaact tatccctgta ggattctgaa agtaactcat agtataaaaa
     5281 cactttattt tatttctctt taataaattt tcaaactttt caatattagg accatccttc
     5341 tccattggaa tttctaatat atttgctctt ctaagcttaa atactaaaag tgctcctcca
     5401 taagttggcc tttctattac aatattatca tttatattaa ctaaggactt acctacaata
     5461 tcaatacctt gctgagctcc agaaattata agtatattat ctaaattatt ttttccattc
     5521 caaaactctt catttatact tcttcttagt tctaaatagc ctaaaggatc actatacatt
     5581 agtgcctcta caccatctct aactaataca ttgtctaaaa ctttcttaag tttttctact
     5641 tggaagaagt ttgcactagt agtttcccca gtaaagtcta tccaattttt taaatctcca
     5701 gaggctattt ttctaaataa atcattataa tcatgcttaa aactttttcc tacctcctta
     5761 tttttagcat aagttccact tccttgggat tgatatgcat atccttcttg tacaagtttc
     5821 ttataagcat taatgacagt taccttatta acttctaaaa agtctgctaa ctctcttata
     5881 gttggtaact tttctccttc tgatatttcc ccattttcaa ttaaactttt aatattatcg
     5941 tatattatta agtatttaca taaatcctct ttaaaaacca cactaaattt cttcatctct
     6001 tacaccttat ttaaacatta tcttttatta aatatacttt ttctgttata agcttttggc
     6061 cacatggtga gaatttttct cttccatttt tcttaactat atttttctta taaagatatc
     6121 ctaataattc ctcaaaatct attttaaacc cttggaattc ttctgatttt tttatttctt
     6181 ctgaacttaa ataatctttt tgttttttca taaaatcagc taaagctctt gttattcctc
     6241 taatatttct ctctaaaaag ctttcaatat aaaaatgtac atattctaaa gcctgtttat
     6301 ctgatgcttt gaatattcca ttaaccattt tctcaaagtt ttcatctaat ttcataagca
     6361 taccagtaga gctctttgtt attaaatatc cattaaaatt taaatatagt ctactaaaac
     6421 tatctactga atacataaga gcttcaaagg cagcgtagaa atgttcatta tgaatacatt
     6481 tcttatataa attcatatct tgtaaaagct cacttaaata aacaaggttc cacttttcac
     6541 tatgagcatc tgtataatat tttattaaag taaatttatc tgatatttct tggtccttgc
     6601 aaaaaattaa ttttgaagat atgaattttc tatgtattgt gcttcctgtt atttcttctt
     6661 gacagaaatt aactagaaac tttttagata ctatttttaa atgtaaagat acgcctagtt
     6721 cctcaccata aacatccttt actcctttaa aatctccatt tacaataaca aataaatcta
     6781 tatctgaccc ttcccatata tctccattta ttatacttcc aaatgccgtc actccaataa
     6841 catcctcatt cttttgaaga tcatttataa ctgttttaaa agaattttga tatttcaata
     6901 ttatttcact caaatttttt tcctccttaa taactcccat taattaaata atatctataa
     6961 acaattccct ttttccacta aaacttttaa agactctttc aataaacaaa ttccaacctc
     7021 catctgttct aacatttcat aagaaatatc taaagctttt tttccttctc ctttttttcc
     7081 ataagtatta gattgataaa ggttagctgc tgccgctgta ttttgtattg caaaacttaa
     7141 aaaataaata atgtccaaag ctggtctaac taccctatca taatacattt tatattcgtc
     7201 acctatattt aatgtagcta cacttgcttg aatagtagct gcttttaata aaatatcctg
     7261 taactgatta tataccttat taaaatcatc tgcacataca ttattacttt cattattact
     7321 tgcagctgct tgagctgcaa aaagatcttc ctgcccatta gagtttcctt atctttctct
     7381 acgccctctt atgtttccac gatctaaagt atccgtagtc tccatgattc acatcctaaa
     7441 aattaatatc attattatta tacttatata tattataatt tatgatgtga aaaatgtaat
     7501 aataaaagct aaattcttgt tgaatttagc ttttactcat gaatttaata ctcttattat
     7561 aactcgtatt ctctacattt attttctact tcttcatcca cttctgcttt tatataagtt
     7621 ccatcttctc tatattcttc ttcacttaca ttagtatttc tatgaagata agctacaact
     7681 tgttgagatg tataaggaat tatatattcc ttgcttttta atgtataagg caattcttca
     7741 cttattgctt ctaataaatt atctaggttt attctttccc tagcagaaat ttttactacc
     7801 ttattatata ttgaagaata ttttgcttct aattcattta gcttttcttc atcagcctta
     7861 tcaattttat taagaactaa tatttttgct gattctaaag cttttaattc acctaatact
     7921 ttttctacag ctataatttc ttcttgtgca ttatctgagg aagcatctac aacatggcat
     7981 aaaagatctg agtatataac ttcttctaaa gttgatttaa aagcttcaac taattcatga
     8041 ggaagtttac ttacaaatcc aacagtatca gtcaatgtta tgtccctatt atcaggaagt
     8101 tttatagccc ttgttgtagt atctaaagtt gcaaaaagca tatctgcttc aaaaacctta
     8161 tccttagctt gagtagctaa agttgaagct gattctgcac atagagtatt tcttaaagta
     8221 gattttcctg cattagtata tccaactaag gaaacctgtg aagttttatc cttacttctt
     8281 ctctctcttt gagtttctct tatcttcttt atttttttaa gctcagcttg aagatcatat
     8341 attctttctc ttatatgtct tctatctatt tcaagtttct tttcccctgg tcctcttgtt
     8401 cctattccac caccagttct agacataaca gttccaagac ccataagtct agaagctcta
     8461 tacttaagct gagcaagttc tacttgaatt ttagcttctc ttgtcttagc tcttcttgag
     8521 aatatttcta aaataagagt ggttctatca attaccttaa ctcctaaatt agcttcaaga
     8581 tttttaacct gtgcccctga taattcatca tcaaaaatta ctaagttagc tcttgttaat
     8641 tgtcttatgt tagcaatttc taaaacttta cctcttccta tataaaaaga agcatctatt
     8701 ttacttctat tttgaaatac cttttctaat gtaggaatat cacaagcctt agcaagttcc
     8761 tctaactcat ctaagctttc tcttgtgtca catcctacta aaatagctct ttcactatcg
     8821 tcttcctcaa cattgcttgt tttaataact tcttcaacat gattaatctt atctaaaata
     8881 ttaatttgta aacacttatc tatagttaaa tgaggagctt cttcacttat aagaatctca
     8941 ttttctacat agcaaaatcc taaagaagca tctttagcct tgccttcctc tacagctata
     9001 gccattattg tatcaagctt taatttcata agggcagata tatctaaagc agaaagtcta
     9061 gaaaccccac ttggatgagt atgtattatt ctaactccag ataatctttt ttcgtgtata
     9121 tctattaatg gaatctctac acttgaagaa tctcctatag ctacactaat tacctttcct
     9181 tttctattta ttcctacact aatttctctt tctatttgag aagataatct agaaataata
     9241 tttatcatat cctctgtaca aacactatat tttggtacat ttattttata aagttcttct
     9301 aattctttaa gtactgaatt ccttattcca tcagtatttc catgtatcat atattaccac
     9361 ctcgtgatat ttattatgtt tcttgacaat tttcatttaa ttttatacta taaatacaat
     9421 attgtaaata atacggaata gttaggggag aaaattatgg aagataaaaa tagaaattta
     9481 ctaatttctg aaaatcaaat taaagaacaa atagctattg taggtaaaaa aataactgaa
     9541 gattacaaag ataaaaacct ttatgtttta tctctattaa gaggaagttt catatttaca
     9601 gcggatttag ttagaacaat agatactaaa actcaaatag gatttatgac tacttcaagt
     9661 tatggccatg gtgaagaaag tactggtata gttaaagttg taaatgatat tcctgataat
     9721 attgaaggct tagatgtctt aatcgttgat gatatcatag attcaggtat aacaatggat
     9781 tttgttataa accatgttaa atcactagga gctaaatcag ttaaaacttg cactctttta
     9841 gacaaacctt ctagaagaaa agttgattta aaaccagact attgttgttt tgaaatagag
     9901 gacttattcg ttgttggtta cggattaaac tatggtgatc actatagaaa tataccttac
     9961 atatttaact gggaaaaata attaaaagga ggaatttcct ccttttttaa taggctaatt
    10021 tgtatcttct ctactttctt cattaatacc ttgtagcata tttccacccc acatgctatt
    10081 tggaacttct ccaactatga cagtatctga aattggaatt tgatttacca cttcaacctc
    10141 ttttgattta agtggtaata ttattttaag attagttttt acattaacca aaattttatg
    10201 ccttgtttga tttattcctg cactttcaaa taaggactca taagtaacta ccactcttcc
    10261 tataggctcc attttaacag ttatactagg cccccaataa gacattatat tattttttgt
    10321 tatatatcca attggcatct ttacacctat tgatccaact tcctttatat ccctctgtga
    10381 ttttatagca acctctgtag ctaactcatt taattttata gtgtcagcat taatcatttt
    10441 tattgcccca tatgaatctt tttgaacttt tatcacatca ttataattaa atttttctgt
    10501 ataaacctca gcaatattag tatttataat atctaaagtt ttagcttgca tttccgaatc
    10561 tgaaacaagt aaaactgtag gtgcaattat tttatcaaaa gcatatatga aaattaaaaa
    10621 acaggcaata atcattaata aaataattat taaatatttt ttaaaatcta taattttact
    10681 tttattttta tttacataat acaaagacta cacctcttat aatttggttt actcttaata
    10741 ttattatgct ataatataat ataatatttc tttaaatata taataatata attatgtagt
    10801 tttttagaaa tagaaagttt tattatgata ggaggaaaaa atgactgaaa gaaaaagaga
    10861 gtataaagat agaaagcaga agaaaaattc acctaaaaat caatcgaaag taacaaaatt
    10921 tttgaaatgg ttctttatag gggttctgct tctagggata actgccataa caatagttgg
    10981 gttttatgtt ctttctatta tacgttcatc tccagaatta gatgttcagg caattcaatc
    11041 tctaaatcag ccatccattc tttacgatga tcagggaaac tttatggata atgttataac
    11101 tcgtgaacaa cgttatgtgg ttaaatctga tgagatacct gataacttaa aaaaagcttt
    11161 tgtagctatt gaagacgaaa gattttatga acataaagga atagacataa agaggattgc
    11221 tggagtaata gcttctaata ttaaaggtaa actctcagga agtaatacag ttcaaggagc
    11281 ttcaactata actcagcaac ttataaaaaa tgctgtgctt accaacgaag ttagttacga
    11341 gagaaaaatt aaagaaatgt acttagcttt agaattagaa aaacatcttt caaaagatga
    11401 aatccttact acgtatttga atacaattcc tatgggcgga taccaatatg gggttagcgc
    11461 agctgctcag agatttttca gcaaaaatgt ttcagattta aatttaattg aatgcgctta
    11521 tttaggaggg cttactcaag cacctacttc ctatgatggt ctctcagaaa taaataaaga
    11581 aaatccaagt agatatttaa atagaactaa atcggtacta tttaaaatgc atgaacttgg
    11641 ctatatttca agtgaacaat ataacaatgc gataaatgaa attgatacaa ctggtataaa
    11701 atttaaacca aataataaat taagtaaaac taattttgaa tggttcacaa gaccaactat
    11761 aactcaagtt aagcaagatt taatgaataa atataaatat acacaagatg aagttgataa
    11821 acttatagct aatggtggat taaaaatcta tacttctatg gatagaaatc ttcaaaataa
    11881 cgttcaaaaa gttttagatg atccaaataa ctataaagct ataactaata atcctaatga
    11941 aaaaaatgaa gatgatgttt ataaattaca agcatctgct acaataatag actataaaac
    12001 aggccatgtt aaagctttag ttggtggaag aggagagcaa cctgctatgt ctcacaatag
    12061 agcttattat gatttaaaat ctataggttc tgcaacaaaa ccattaacag tttatggtcc
    12121 tgccattgat ttaggacttg gtggtgctgg atctgtagtg aatgattctc cgctaactaa
    12181 taaagaacta tcttctactg gatataagga tcaacctaag aatgaatata atagctatag
    12241 aggaccttta actttcagag aagcaattaa aatctctagt aacttagcag ctataaaagt
    12301 tgctaatgaa gttggtgttt caaactctat agcttatgga gaaaaattag gtcttgttta
    12361 tggacctcat tctagaggta tttccacaac tgctttagga caatttcaaa atgatcctaa
    12421 taatcctgat ggaggaaata cttataccct tgcttcagcc ttcggtgttt ttggtaataa
    12481 tggtgttaaa acaaatgcta aattatatac aaaagtatta gattctcatg gaaatgtact
    12541 tcttgataca agtactccag aagaaattaa aatatttagt cctcaaactt cttatatagt
    12601 ttatgatatg cttaaagatc aagtagaaag tggctctgca aaacctgcta aatttggtaa
    12661 tattcctgtg gctggtaaaa caggaactac tactggagat aaagactatt tatttgcagg
    12721 attaactcca tattattctg ctgctatttg gattggatat gataaaccta gagaaatgag
    12781 aactagtagt ggtatagtta cgtcccctat tttcggaaaa ataatgggat tagctcataa
    12841 aggcctacag tataaagagg ttgaacaacc tagtggaata agtaagattg ctgtatgtat
    12901 ggattctggt cttaagccta cgtctctttg tactcaagac cctagaggca gtagagttta
    12961 ttatgattgg tttattaatg gttctgctcc taatcaatat tgtaattatc atacaaattc
    13021 tcatgtaaat aattttaata aaaataataa tactactgga gtaaatgaaa aaaataaaaa
    13081 gcaagaaact aaaaccgaag aaattaaacc caacgaaaat aataaatcaa atgaacaaac
    13141 tccaaatact aattctgata atgcaccaaa taatgtgcca aataatgata ataacaatag
    13201 caataacata ggtggaaata tgattccccc tcaaaatcaa acagaaaata atacaaataa
    13261 tggagttata actccaaatc aaagtataaa taataagaat tagtaatatt aaaataacac
    13321 tattacgcaa cagaataaag attttcttgc attttaactt tatgaaaaat aaagccacta
    13381 gaaattttct atttcatagt ggctttatta ttgattttaa tccctctata tctttgtaat
    13441 atagtactat tcattaatga tacctcttta ctttttatta acatttaatt cctctatttt
    13501 tcatttaaac atctagcata tgaataaatc aatattagtt cttgcatttt tttattattc
    13561 atattaaaat tagatataaa gtgtgacgta aaaaataaaa aacttatcaa caggaggagg
    13621 aatttatgga aatacttaat aacttaatag ttagttttaa tgattttctt tggggctaca
    13681 tattaattat tttattaata tgtgcaggcc ttctattttc ttttaaaact aaatttgttc
    13741 aatttagata tacaaaagaa atgttccgtt tattaggaga cggtatagga aagtcaaata
    13801 aagaaaaagg cggagtttct tcatttcagg cattttgtat aagtacagct tcaagagttg
    13861 gtactggtaa tatagcaggt gttgccatag ccgtagctgt tggtggacct ggggctgttt
    13921 tttggatgtg gcttatagcc ttaataggtt ctgcgtctag tttcgtagaa agtacattag
    13981 ctcaaatata taaaactaaa gataaagatt cttttagagg tggtcctgcc tattacatgg
    14041 aaaaaggact tggaaagaga tggttaggaa ttatattctc aattcttatt acaatctctt
    14101 ttggattcgt atttaatgca gttcaagcta acacagtaag tgttgcattc aataacgctt
    14161 tcggactttc aagagaagca attggtataa tattagtaat agtaactgct ttagttatct
    14221 tcggtggcat tcacagagtt gctaaagttt cagaaataat agttccaatt ttagcggttc
    14281 tttatattct tattgctatt atagttttaa ttttaaatat tactgaaatt ccatcagttt
    14341 tcaaacttat atttgaatca gcctttggaa taaaacaatt aactgccggt ggacttggtg
    14401 ccgctgttat gcaaggtatt aaaagaggtt tattctcaaa tgaagctggt atgggtagtg
    14461 caccaaatgc agctgctaca gcagatgtta ctcacccagt aaaacaaggt ttaattcaaa
    14521 ctttaggagt atttactgat acattattaa tttgttcttg tacagcattc ataatacttc
    14581 tttcaggtca atatgccgat agttcactaa caggtataga attaactcaa aaagctcttg
    14641 tatctcaaat aggacctttc ggagcttatt tcatagcaat ttgtatatta ttatttgctt
    14701 ttagttctat tgtaggtaac ttctattatg gtcaaacaaa catagagttt atacatacta
    14761 ataaaatatt attaaatata tacagaatac ttgttatagg tatggtatta tttggttcaa
    14821 tagcaaaagt agatttagtt tggaatttag ccgatctttt catgggatta atggctatac
    14881 taaatataat aactatatta ttccttggta aatattcttt ccttgcactt aaggactata
    14941 cagctcaaaa gaaagcagga attaaagaac cagtatttaa agcatcttct attcctggat
    15001 tagaaaatgt ggaagaatgg caataataaa aaagctttga gatttttttc tcaaagcttt
    15061 ttatattaat ctttcaaata taatcttttt ctccacactt aaatctttag gatttattgt
    15121 tattttaaaa attggaggac acccttcctc tctccatttc ttcatataag ctggtgtagc
    15181 tttataatta taataataaa cttcttcata ttcatctgaa aattctaaaa cttcaagtct
    15241 actatcttca cttaaactta ttaattcttt atgaacatta tttattattg gccaaaaata
    15301 cttcattggt atattgtatt taaactcatt ttttactact ctgctctcaa acaatgctct
    15361 ttttaatatc ctattctctt tatgacactc atctatattt aatctaaata taacttttcc
    15421 tcttttgtct tcatctaatt ttatgtttat catataataa acaactccta ttcataacac
    15481 tataaaatat agcatcaaat ctataaatca aatctattta ataaagattc tccaaacaat
    15541 tttctaatta tgtattttat tccttctatt tcacaggatt ttttcttaac cattataagt
    15601 cttccattat caataatatc ttcatcagaa tacgatatta aagcaagttc ttcttttatt
    15661 aattcaaccc attctttaga ataatatgtc tttaatcctc ttaaaaattt attttttata
    15721 aatggaatta tactattccc aataataaat acaatttcac atccatttat attgtttaag
    15781 agcttttcaa actcctcgtt ttcataaaac ttattactct tacttccggg aacaattata
    15841 acatctagat ctacttcctc tatagatttt acattttcaa actttatatc atacattcca
    15901 tcttttatta tattattttt tgcacctact aattccactg aaattatctc tttatctagt
    15961 gatttattta tatcatgaaa aatttctaag aaagatatta ctgtgcttac taccatctta
    16021 tcataaataa atattccaaa ttttctctct tttaagtctt tgatttgact attaaaatct
    16081 tccttagata atctatatga ataaccatct ttatttttat acataatctt gaaaccaatt
    16141 ttttcaagtc tttctctatt ctcatctaac gaaactgtat caagcatata taaaccacca
    16201 aattcattga aaaagtattc taaaataaga tataccgctt cacttccata accttttccc
    16261 ctatgctcgc tttctattct aacatttaac ctagcagttt tagttataga gtcatatcca
    16321 tgaaagcttg cttcacccac tggattgtca ttataatcaa aaaccaagca atatagattt
    16381 cttccatctg tagggctcac catcttttta taaaaactat cccacttata cctaggaaaa
    16441 gatattgttt tgcctatatc agtcatactc atcttatctc cccatagctt ttcggtataa
    16501 cttagttctt ctaaatatgg tgttcttata taaacctttc ttccttctct cctataacag
    16561 tttctttttt ttatcacatt accacaccaa tcatttatta attcaagagt attttatcac
    16621 aaattaccac aaactgtgaa attttttata tattatttat ccattgttac tttattgttt
    16681 agatatacta aatttaatag tagattgctc taaacttcat aaaaataccc ccttctaaag
    16741 ttgctaaata actattttaa gataatattt aaatattaat atatttttat ttttttgttg
    16801 atttttattt taaaagtatt tataattatt tatgtttgtt tagtaatttt aaacttaaag
    16861 tttagtaaaa cttatattaa tgaaattgag gtgtaaaact tggaaatagg agataaaatt
    16921 agacgtctca gagtggccaa acagttaact caagaagaat tagcaaatag atgtgaatta
    16981 tctaaaggat ttatttctca gcttgagaat gaccttactt ctccatctat agctacatta
    17041 atagatatat tagatatatt aggaactaat ttaacagaat tctttagtga agatactaat
    17101 gaaaaaatag ccttctctaa ggatgatatg tttgaaactg aaaatgaaga attaaaatat
    17161 aaccttatgt ggttagttcc aaatgcccaa aagaatgata tggaacctat aatgataact
    17221 attaaacccg gaggacaata tattgaagaa gagcctcatg agggtgagga atttgggtat
    17281 gtcctatctg gagcaatata tcttcatcta ggagaaaaaa aagtaaaagt aagaaaaggc
    17341 gagagtttct actttaaacc caaggctaat cattatattt ctaatgcagg aaaaatttct
    17401 gcaaaggtta tatggataag tactccacca tcattttagt ataaacattt agaaagcaga
    17461 ggtgttagat ttgaaagata ataatattat tgaattaaaa ggaataacta aatcatatgg
    17521 aaaagataca attttagata atctttcctt aaatataaaa aagaatgaat tcctaactct
    17581 tttaggacct agtggatgtg gtaaaacaac cactttaaaa attatagctg gttttgaaac
    17641 agcagatagt ggccaggtgg tctttgaaaa taatattatt aacgatatac caccttacga
    17701 aagacaatta aatactgtat tccaaaaata tgctctgttc cctcatatga atgtgtatga
    17761 aaatatagct tttggattaa aactaaaaaa aattcctaaa gatattatag atcaaaaggt
    17821 taaagaaatg cttaaattgg ttgcccttga aggatatgaa aatagagata ttgaagccct
    17881 tagcggtggt caacaacaaa gggttgccat agctagagct ttagtaaatg aaccaaaggt
    17941 acttttatta gatgaacctt taggtgcatt agatatgaaa cttagaaaag aaatgcaaat
    18001 agaacttaag aaaatccaac aaaaattagg aattacattt atatttgtaa ctcatgatca
    18061 agaagaagct ttaacaatgt cagatacaat agttgttatg aataaaggtg aaattcaaca
    18121 aatgggaagc cctgaagata tatataatga acctgctaac tcatttgtag caaaattcat
    18181 aggtgaaagt aatatagttg atggaattat gttagatgac tttaaggttg aatttgatgg
    18241 aaaaatattt gactgtgttg ataaaggctt tgaaaagaat gaagctattg aagttgtaat
    18301 aagacctgaa gattttgaaa tggttaaata tgaaaatggt atgttaaaag gaactgttac
    18361 ttcaattata tttaaaggtg ttcactatga aatagaagta aagaatgaaa atcacacttg
    18421 gatattacac aatacaaaac atgctgaaat aggatctaaa atcggattat cgcttgatcc
    18481 agaaagcata cacataatga aaaaagagag tgatgtttaa atatgaaaaa tagaaataag
    18541 aatttatcat ttgcagctta tccatacgtt ttatggagtg ctatattcat agttatccct
    18601 ttgcttttaa tagttttctt tagttttaca agtaaagttg atggaagatt tgttttctct
    18661 tttgaaaact tccaaagatt atttgagccc atatatttta ctgtttttat acgttctata
    18721 tggcttgctg ttttatcaac agttttatgt ttaattttag gttatccaat agcttatatg
    18781 atttctaaat tgcctataaa aagaagaaat atgttaatac ttttatttat attaccaatg
    18841 tggatgaact tcttacttag aacatatgcg tggatggcta tacttggtag agatggacta
    18901 attaatactc ttttaggtta tataggaata ggtccaatta aaatgctata tactgacgga
    18961 gcaatcttac ttggaatggt atataacttc ttaccattta tggttatacc aatatatact
    19021 gtacttataa aaattgataa aaacttagtt aatgcagctt atgacttagg tgcaaataaa
    19081 gcccaagctt ttagaaaagt tattttacca cttagtattc ctggaataat ctctggaata
    19141 actatggtat ttatgccagc tgtttcaaac ttcgtaattc caaaccttct aggtggtgga
    19201 aaatatatgt tagttggtaa ccttatagaa caacaattta ctactatcgg taactggaac
    19261 ttcggttctg ctttatcaat ctttatgatg atattaatac taatttcaat ggcatttatg
    19321 tctaaatacg aaaagaatgg aaaagaagga ggtaaacagc tatggtaaaa aagttaggtg
    19381 attttatttc taaattttat ttaatattag tatttatatt tttatatgca ccaatagtaa
    19441 cacttatgat attttcattt aacaaaacaa aatctatggc tgtttggggt ggctttactt
    19501 ttgattggta cagctcccta cttgaaaatg atagaataat atcagctctt tattatacaa
    19561 ttttaatagc agtaatagcc tctgttattg ctacagttat aggtactata agtgctatag
    19621 gaattaataa tatgaaaaaa ggtcctagaa ccttattatt aaacgtaaac tatttacctg
    19681 tgttaaatcc tgatatagtt acaggtattt cattaatgac tttatttgct attggtggag
    19741 ttttatttaa tttaaaacct ggtttttgga ctatgctttt agctcacata acatttgata
    19801 ttccttatgt tatattatct gttttaccta agctaagaca attgcctcca aatctaactg
    19861 atgcagcttt ggatttagga gcaaaaccat cttacgcttt aagaaaggta gtaattcctc
    19921 aaataatgcc tggtatagca gcaggattct taatagcatt tacaatgtca atagatgact
    19981 ttgttataag tttctttact acaggtcctg gtgtttctaa cctttctata gaaatttatt
    20041 ctatggctag aagaggtatt tctccagaaa taaatgctct ttcaacatta atgtttatag
    20101 ttgtcttagc attacttctt attgctaata gaaaacaatc tttagtaaag gaggaacgtt
    20161 aatatgaaat taaaaaaatt tttagcatta acttgttcaa cacttctttt atcatctctt
    20221 tttcttggtt gtggaccaaa aaaggatgag gaagctactc aagataaaaa caataacgtt
    20281 ctttatgttt ataactgggg agattacata gatccagaac tacttactaa atttaaagaa
    20341 gaaactggaa tagatgttaa gtacgatgtt tatgatacta atgaaataat gtatcaaaaa
    20401 cttaatagtg gtaatgtatc ttatgatata gtaattcctt ctgattacat gatagaaaaa
    20461 atgaaagaag aagatatgtt agctaagata gattattcta atattcctaa ttataaatat
    20521 ataggagaac aatttaaaaa tttagcttat gatccaacta atgaatactc agtaccatat
    20581 atgtggggaa cagttggaat aatatataat acaaagaaag ttagtgatcc tgtagatagt
    20641 tggaatatcc tttggaaccc taaatataag gatcaggtta taatgccaga tagcgttaga
    20701 gatgctatgg ctgttgcaga aaaaaaatta ggatattcat taaatactga aaacttagat
    20761 caaatagaag ctgctaaaaa ggaacttatg actcaaaaaa aagatggatt aatcttagct
    20821 tatatggttg accaagttaa agatgccatg gttggtggag aagcttccct tgctgttgct
    20881 tggtctggag atgctgtaac aatgatagag agaaatcctg atttagctta tgctattcct
    20941 aaggaaggat caaataaatg gtttgatgct ataggaattc ctaaaaatgc aaagcataaa
    21001 gaaaatgctg agaagtttat aaacttcctt tgcgattctg aaaatgctga gcaaaatgta
    21061 gaatatatag gatactctac tccaaacaca gccgcatatg accttttacc tgaggatata
    21121 agagataata aagttgcata tccagataaa gagtcattaa aaaattgtga ggtatttata
    21181 gatttacctt ctaaaatact tagaaaatat gatgaagctt ggttagaaat caagtgtatt
    21241 tattaaaaaa ggtctttatc aaaatataac aaataaagct tacaaacagt atgaagagaa
    21301 tatctagatg agctttagaa tttaaattat tagcgaaatc tagtaattct cggaatatgt
    21361 ttgtaagttt tataaaaagt tattttgata gagacccttt tatattatcc acaaatttct
    21421 tcataaaata cttttgggaa ccatagctta atttctttct cagcactttc tattgaatct
    21481 gatgcatgaa ctgaattttc tgttccactt aaggcaaatc ttcttcttat tgttccaaac
    21541 tcagcctttt ctggatttgt tgccccattt agagatctta ttttatctat agcatcttct
    21601 ccttcaagaa ttaaagctac aagaggactt cttgttatat attttattag agaattaaaa
    21661 aattgcttat ctctatgttc ttcataatgt ttttcagcaa aatccttatt tatttgcttc
    21721 atttccatag cctttatctt taatcctgct ccctcataaa cctctaatat cttacctatt
    21781 aaatttcttt caacggcatc aggttttatt aaaacaagac ttttttctaa tctcattgaa
    21841 attcctcctc aatattattt ccctctaaga tatattatac atataacttc ctatttatgt
    21901 aaacattcac aagaaaatta ctaaatattt aattgttaaa ttattatttc caccttagtt
    21961 agagaaataa gtgcctttta tatagtattg taattataat aaatatagga ggtgtttact
    22021 gtgattagaa aagaatttct agtaagtggt agagtccaag gagtaggttt cagattcttt
    22081 tgtaagtatc aggcaagctt attatcttta actggttatg ctgaaaattt agatgatggt
    22141 caagtactta tagaggtgca aggtgatgaa tcatcaataa gaaaatttaa aactaaaatt
    22201 ttaaatggta atggtttttc aagagttatt tctatagatg agaaggattt aactgttgat
    22261 acccgcgaaa aaagattttc tacatactaa ttataggtaa aggaaatggg gctattatca
    22321 aacttaaaat ttataaatat acaataaatg ttattcacta attattataa tatagtttat
    22381 aaattttgtt tttaaatttt taaaagcccc tactcctttt tatagctttt tataaatcac
    22441 agctatcttg ttcatatcca tatcttgagc ttccattgct attaaactct tcattttctt
    22501 tgtatgaaat aaatgtacca tcatctggtt catgatcttt atgtttttta ttattataat
    22561 tcttattatt tttattacct ttcatattta tcacccacct ttaattttat tatttcaagg
    22621 taggcttatt tatatactct atttttaagt tgattatata ttatagtttc ataaactttt
    22681 ttacttaaat tataagtttt tatatgaaca aagtcttaac ttctacctat tgacttaaaa
    22741 atttttactt atattataag ttatcccaca tatcttgtat gctactataa tcatctaata
    22801 cttcatcaat taaatttaaa gcatcttcat tgttccaaga ttcatatttt tttcttttta
    22861 ctactttaat tatgttttct tcttttcctt ctttaattaa aatagaatct tcacaggact
    22921 ctttatactt ttctaaatcc tttttaattg tattacaaac ttcaatagtc ttaattctat
    22981 tgttgtcttc atctctaaaa tctataagta ttttgtctaa gttaattaac tgatttaaaa
    23041 agtcaaaatg ccaattaaat ccataattta accatagatc ataggtattt tcttctcttt
    23101 cgtcaaacct aatcataatt aaaagcaagt ctaatttttt attgcttata atactgcttt
    23161 ttagggcaac taagacatta tcacctatat cctttaaagc ttctaaataa tctttaacaa
    23221 aaattatagg tactacttta tcatttactt ctactattag tggaattaat gttccaactt
    23281 cactttcttt taaaatatca tctacagtta aagctgccat attatcacat cctaaatatt
    23341 aattaattgc ttcaaatacc ttatattata taagatatgt attgtaaata ccacaattaa
    23401 agctttacat ctctttcttt attattattg ttattatgcc ctatagatgt tataataaaa
    23461 aaagtttaat gagactttga aattaggaga gatgaaaatt gattaaattt aagaaaaagc
    23521 ttttaagttc aatattgatt gctttatcag tatcactttt tgcgccaata aaagctactg
    23581 ttcaagctgc tgaaatttcc cagcctaata tagttggaaa gtatgctgtc actttagatt
    23641 atgaaactgg tgaaattatt tatgcaaagg gaatagatga aaaagcatac cctgctagta
    23701 caacaaaggt aatgacaagt cttttatttg ctgaacatgc ttcaaagaat gattcttttc
    23761 catatacagc agatgcaaaa gttcaacaac cttatacatt aaacgatagc tttggaccaa
    23821 tacctgttgg cgaaggaatg aatgctaatg atttaatgaa agctttactt atgttttcag
    23881 ccaatgatgc tgccgctgta attgcagatg gcgtggctgg aagtactgaa aaatttagtg
    23941 taatgatgaa tgacgaagta aagaaattag gacttaaaaa tactcacttt gttactccaa
    24001 acggcttaca taatgatgat cactattcaa cagcttacga tttagctgtt attttacaaa
    24061 atgcctataa aaatccttgg gtaagagaaa ctatggcact taaggatagc gacataactg
    24121 taaatggaaa aaaagtactt ttagaaaata gaaataaaga gcttggcatc aacggaaata
    24181 ttggtggaaa aactgggttt acaactcctg ctgggagatg tttagtgtca gtatacgaaa
    24241 gaaatggtag aaaaataata ggtactgttt taaattctca atatgatgct aaggatgaaa
    24301 ttgtatttaa tgatatgaat aaaattattg attacagtta ctctgtagat aaagttccat
    24361 acataaaggc tggtacaaca atagatacta ttccagttga atataaactt tttagatggt
    24421 ttggaccaac taaaaaaata gacgttcctt ttgttgcaac tgaaaacata gattattata
    24481 aaaattatgt aaatgaaaaa gaaacatcaa aatcaataaa cttaaatgat atgaatgctt
    24541 ggcaattagc ttctaatcct gaatcagctg ctgttactgt tactcaaaga gcttacgtta
    24601 aggattatcc agtaaaagct gatataggta cttttactct tataaaagct aatttcttaa
    24661 gttatttagg aataattgtt ctagtagcta ttgcgattgt attaatatta cttattataa
    24721 gagcaataaa tttaagaaaa cgtaaaaaac gtagaagaaa tatattttaa taaaaaaaaa
    24781 gatttagtct aaggctaaat ctttttttta ttaaaaacct attaaattag gaatttaatt
    24841 tttcaacaag atctaaaatt tctaaaggtt tatctacata aaagtctggc ttactctctt
    24901 ctaatttttc aagaggcaat gctgtatatt taactccaca agtcttagcc ccagcgtttt
    24961 tacctgctaa aatatcgtaa ggtgaatcac ctaccataag agcttcacta ggagatactc
    25021 ctaaattttc acaagccttt aaaacaggct ccccatctgg cttatgcttt gtagtgattt
    25081 cagaagttat aaaagtatca aaatacttaa atatacccat taatttagct ccacgttctg
    25141 ccatatcatt tttctttgaa gttacaactc caattttaat tcctttgctc tttaatgttt
    25201 ttaacattaa atcgactcca gcaaactctt tacattcatc atcatgtaat tctaaattta
    25261 tttttctgta acaattaatc atttcttcta ttctattttc atcataggat ttaaaaattt
    25321 cctgcaatgg tctaccatag ttcatagtaa tctcttcttc acttggctct aaatctaata
    25381 tagttttaaa tgtatgttta aatgatttta gtattaaatc attagtattt atcaatgttc
    25441 catctaggtc aaacaatact gcttttatca taaaattctc ctttattatt ttaaaaatta
    25501 gctttaaaat atatatattt ttaat
//
LOCUS       DAEVGI010000021        23815 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEVGI010000021 DAEVGI010000000
VERSION     DAEVGI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 23815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23815
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.021"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(125..1066)
                     /locus_tag="K8M77_000817"
     CDS             complement(125..1066)
                     /locus_tag="K8M77_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7085331.1"
                     /translation="MYSFHKFLEELYLNTKIPFIVVLDDKKFVLSDFDNYDNVSLELE
                     VNGKKGKIITQKEYESCTSLLRYLIENKIKEVSSNKEKLIRKALNGVTIDKNIIHKEL
                     GFLKGDFYIINVYLASNLQEGLELVNECYGNKNIVSILKEDNIVIIGALEDVDDHVNS
                     IKDSIDSNLYVKCYISYMKLNNINEIKEAYEINMSKINLAIKYDLDEVIYGERDLLFE
                     TLVDSIDEDLKMKMYEDFGLGFSKLDREMIKTIETFFKCGLNISDASKELYVHRNTLI
                     YRLDKIQKCTSYDIRNFNEAVIFKIAFLIWRGKEINK"
     gene            complement(1101..2210)
                     /gene="ugpC"
                     /locus_tag="K8M77_000818"
     CDS             complement(1101..2210)
                     /gene="ugpC"
                     /locus_tag="K8M77_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBI7085332.1"
                     /translation="MANLSLKHIYKVYPGDVTAVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISKGELYIGDRLVNEVEPKERDIAMVFQSYALYPHMTVYDNMAFGLK
                     LRKVPKDEIDKKVKDAAKILDIEHLLDRKPKALSGGQRQRVALGRAIVREPKVFLMDE
                     PLSNLDAKLRVQMRTEISKLHQRLQTTFIYVTHDQVEAMTMGTRIVVMKDGIVQQVDS
                     PQEIYNNPANIFVAGFIGSPQMNFIDGTIKEEGGKYFACFQSEKIEMPMDKARLLKEK
                     GYIGKNVIIGIRPEHLDDDQELVAANPTTVIKSKVEVTELMGAESYIYTKLGDQNITV
                     RVNGSTKLQNGQEAKFYVDANKIHIFDKETELKLV"
     gene            complement(2427..4145)
                     /locus_tag="K8M77_000819"
     CDS             complement(2427..4145)
                     /locus_tag="K8M77_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBI7085333.1"
                     /translation="MNKIIINDKTIEFDGDKTILDLARENGFDIPVLCELKNCGNKGQ
                     CGVCLVEQEGNDRLLRSCAIKAKDGMVIKTDSEKVLEARKERVAELLDEHEFKCGPCK
                     RRENCEFLKLVIKTKARAHKPFVVADKSEYVDDRSKSIVLDRSKCVKCGRCVAACRTR
                     TATNSIKFHRIDGVRLVGPEELKCFDDTNCLLCGQCVAACPVDALSEKSHIERVQDAL
                     NDPEKHVIVAIAPAVRTSMGELFKMGYGQDVTGKLYTALRKLGFDKVFDINFGADMTI
                     MEEATELIERIKNNGPFPMLTSCCPSWVREVENYFPELVENLSTAKSPQQIFGSASKT
                     YYPQVADIDPKKVFTVTVMPCTSKKFEADRPEMENEGIRNIDAVITTRELARMIKAAK
                     IDFAKLEDSEVDPAMGEYTGAGVIFGATGGVMEAALRTAKDFMENDNLDNVDYEAVRG
                     LAGIKEAEVEIAGNEYKLAVVNGAANVFELVKSGKINDYHFIEVMACPGGCVNGGGQP
                     HISAEDSDKIDIREVRASVLYNQDKNLEKRKSHQNSALLKMYENYMGKPGHGRAHELL
                     HMKYKK"
     gene            complement(4371..5441)
                     /gene="buk"
                     /locus_tag="K8M77_000820"
     CDS             complement(4371..5441)
                     /gene="buk"
                     /locus_tag="K8M77_000820"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBI7085334.1"
                     /translation="MAYKLLIINPGSTSTKIGVYEGEKEILEETLRHSAEEILKYDTI
                     FDQLDFRKEVILKVLKEKGIDINELDAVVGRGGMLKPIEGGTYEVNEAMVEDLKIGVQ
                     GPHASNLGGILSNEIAKEIGKRAFIVDPVVVDEMEDVARLSGVPELPRKSKFHALNQK
                     AVAKRYAKEHNTSYEDVNLIVVHMGGGVSVGAHRKGRVIDVNNALDGDGPFSPERAGG
                     VPSGELLEMCFSGKYSKEEVYKKLVGKGGFVAYANTNDARDLIKLSQEGDEKGSLIFN
                     AFIYQIAKEIGSMAVVLDGEVNAIVLTGGIAYSDYVTNAINKKVKWIAPMVVYGGEDE
                     LLALAQGAIRVLDGVEEAKIYK"
     gene            complement(5466..6374)
                     /gene="ptb"
                     /locus_tag="K8M77_000821"
     CDS             complement(5466..6374)
                     /gene="ptb"
                     /locus_tag="K8M77_000821"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBI7085335.1"
                     /translation="MAKNFNELFSKLRENSMKKVSVAVAQDEPVLEAVREAKELGIAE
                     AVLVGNEEKIKEIAKKIGMNLADFEIINEPNDKKAALLAVELASTGKTDMVMKGLVDT
                     ATFLRSVLNKEVGLRTGQLMSHVSVFEIPGMDRLLFLTDAAFNTYPELKDKVSMINNS
                     VKVAHACGVEVPKVAVIGAVEVVNPNMPATIDAALLAKMNDRGQIKGCIVDGPFALDN
                     AISEEAAEHKGVKGEVAGKADILLLPNIDVANVMYKTLTYMSECKNGGLLVGTSAPVI
                     LTSRADSFETKVKSIAIAAVVAHDLK"
     gene            complement(6557..8158)
                     /locus_tag="K8M77_000822"
     CDS             complement(6557..8158)
                     /locus_tag="K8M77_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085336.1"
                     /translation="MTIRINNISLDINEDLSLLKNKVAKKLRMSKDEIGDIKIIKESL
                     DARKKNLIKFNYCINIEHEDEEKIVARLNDKDVKIDKVNYDFDFTFGEKKLQHRPVVV
                     GLGPAGLFAALLLAKKGFNPIVFERGEDVDSRTKTVEEFWRTGELNPESNVQFGEGGA
                     GAFSDGKLTTRIKDTRCDYVLDALVRNGAPEEIIYKGKPHVGTDILKNVVKNIREEIK
                     RNGGEVHFNSRFEGIIKKDNKLKGIKVNGEEVPCEVAILALGHSSRDTYEMLFNEGVF
                     MKQKPFAIGVRIEHPQEIINLSQYGEKYANHPRLKAAEYRLAYQSKTLDRAVYSFCMC
                     PGGVVVNASSEEKRLCVNGMSYHARDKENANSALVVTVGPNDFGGDHPLEGMKFQRHY
                     EELAFKLGGENYNTPVQLVGDFMKDRVTTKLGKVNPSVLSNGYRFEDLRKCLPSYVID
                     GLKEGITDFDRKIKGFGHSDSVLTGIETRTSAPVRIERNEKLQSISLEGLYPAGEGAG
                     FAGGIVSAAVDGLKVAENIMKEYHV"
     gene            complement(8234..9556)
                     /locus_tag="K8M77_000823"
     CDS             complement(8234..9556)
                     /locus_tag="K8M77_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085337.1"
                     /translation="MLKKIEGKNFLVKFICLLLSFSLWLYIINVENPVRELKLNNVPV
                     QVVNSEVLKDYDLVMVPNQNLTVDLDLEGPSTEIYKVKKEQFKVVVNLSEYVLKEGDN
                     NIPVQIESYPNNINIKNNGFLRVNVILDKYQEKSLPIVSKIKVNTEHGTYADKINISP
                     QNATVSGAQSLVSKVKTLEVKGEINDVSKAVNMNLPVVAVDGDGNEIKDVNISPNKVD
                     VSFGVKKSKEVPVSVITTGTPKDGLALKSITPSIAKVTLLGSEDALSKISSIETSPID
                     VSQYGDDSEVSTVLKIPDGVSLASNDQAQIKVKLDFSKDAQKEIEVPVTTEGTLDGYT
                     PELDTKTIKVTINGPEDSVNSIDLSKFKCTIDISKLTADGGSVKPNISNSYDNIKVVS
                     INPTEVKVTFKKNTNSTESTENNSTKPSDNSNNNTSKGSNNENSNNKQ"
     gene            complement(9523..10380)
                     /locus_tag="K8M77_000824"
     CDS             complement(9523..10380)
                     /locus_tag="K8M77_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBI7085338.1"
                     /translation="MQELIMIFTNSLKDISIWSIIDILIVAFIFYRGYILIKETRAEQ
                     LLKGVILLLILIPISYVLRLQMLNFILTKTLTIGVLSIIIIFQPEIRRALEHIGSTAF
                     DDFHVIQDDQKLEEVIDQLIVAVEDMAETKTGALIAIEQGTGLAEIISTGTQLDAVIT
                     SALIENIFFKNTPLHDGATIIRNDRIVSAGCVLPLTNNNTINKKLGTRHRAAIGLSEI
                     SDALVIVVSEETGAISLAVKGRLTRNYDGKKLKNILLKVMRNRRDKRGKTYGEKVKIC
                     LKKLKERIS"
     gene            10541..11503
                     /locus_tag="K8M77_000825"
     CDS             10541..11503
                     /locus_tag="K8M77_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085339.1"
                     /translation="MDFKYKKYIDNSLIYIIFAVLILIFIIGFLFFRSHNEKSKLEDL
                     GRTISEINLTYDFSEDSITSEDYVSSIENKLKKLQETNSALKSLKVSQKHQNLSSPLK
                     DGLDKNIELFNKLLSILKTPDALNISDEYAIASKLKKECENYYSICRSNLIPVYLYKE
                     GNNYLQDIFYYINELIKTNRDKGFVQSQKNDFVVSMKSLLLKLSSMDEKLFETANLIK
                     ESNRDLKVLVTDIENKLSSLQELKNNLYSLPIPEQANDSFLALEKALKSYDKYINYFY
                     KGLLDEIENKKTPEDYYDKSSTLFDEFIYSLEAAEKSLDNYNKN"
     gene            11574..12176
                     /locus_tag="K8M77_000826"
     CDS             11574..12176
                     /locus_tag="K8M77_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBI7085340.1"
                     /translation="MKKSIYNYFIFTFALLIITTLNEKEVLGLSLSNEHVTKNNYIEL
                     TEMASNSPNPEAEQSSNSNNEVIAVFQSSNSTISLTKDDIYLMSQVVYAESKGEPFDG
                     KIAVASVILNRTTDSQFPDTIHGVITQKNAFSCVRNGKIDVVPDGDSYNAVLKAIEGY
                     DPTDEALYFYNPKIATCSWMKGVEKTGEKIIGQHVFFNVT"
     gene            complement(12208..13068)
                     /locus_tag="K8M77_000827"
     CDS             complement(12208..13068)
                     /locus_tag="K8M77_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI7085341.1"
                     /translation="MSERYDIAIIGSGPAGLSAALNAKIRNKKFILFGNNDLSTKLVK
                     APKVNNYLGFVGKDGMDLKNQFMGHLKEMDIEITEERINSIYAMGEYFALMVNEKMYE
                     ATSIIIATGIEYTKPMKGEEEFLGKGVGYCATCDAPLYKEKTVTIIGYNKEAEEEANF
                     VSELAAKVYYIPRYKGEFDLNSEIEVINDIPVEIQGDSKVTKLVTKNGEIETDGIFVL
                     RDSISPGQLVPGLKITDDHIEVDRLMKTNIEGCFAAGDCTGRPYQYMKAAGEGNVAAL
                     SAVAYLDSKK"
     gene            complement(13095..13409)
                     /gene="trxA"
                     /locus_tag="K8M77_000828"
     CDS             complement(13095..13409)
                     /gene="trxA"
                     /locus_tag="K8M77_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBI7085342.1"
                     /translation="MVKHINDQDFNTEVIEASEVVVVDFWATWCGPCKMIAPVIEELA
                     NEMQNVKFVKVDVDKNPGSAGKYQIQSIPTLLIFKDGKVVDTLVGFRPKAALVEAIKK
                     HV"
     gene            complement(13526..14695)
                     /gene="iadA"
                     /locus_tag="K8M77_000829"
     CDS             complement(13526..14695)
                     /gene="iadA"
                     /locus_tag="K8M77_000829"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI7085343.1"
                     /translation="MLKVIKNIEVYDPEYLGKKDIVILSDKIEGLYDSVNIPKDFINI
                     QVIDGEGMIAVPGFIDCHVHIIGGGGEGGFRTRTPELQLSSIIKGGITTLVGCIGTDG
                     VCRDMKGLVAKAHALCEEGVTCYCYTGSYDVPVNTITKTIKSDLLLIDKVIGVGEIAL
                     SDHRSSQPTYEQFVNIVAQARVGGLLSGKAGIVNVHLGNGKRMIEYITRLINETEIPA
                     NQIIPTHIGRSRELFEAGINHAKAGGQIDLTTSSDPQHLEEGEVRAGEGLKKLLDAGV
                     DIERITFSSDGNGSMPLFDENGELKGLGICSVESLFNEVKEAIKLGVPLEKAIRVITS
                     NVANVLKLSHKGFISTGKDADIILLDKKNLDINSFISRGNILMDNKKLLVKGTFE"
     gene            complement(14860..16479)
                     /gene="ptsP"
                     /locus_tag="K8M77_000830"
     CDS             complement(14860..16479)
                     /gene="ptsP"
                     /locus_tag="K8M77_000830"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI7085344.1"
                     /translation="MKKGIAASKGYAIGTVFIQEHEEIIISDAKVSDIAAEKEKLSKA
                     LAQSKEQLEAIKEKTANEIGEHEAQVFEAHLMLLDDVEFTGQMEMTIENDQLNAMKAV
                     QNVTDTFVMIFDSMDDPYMRERAADIKDVSKRIIANLAGKGGNGMENVGANTVVVAHD
                     LTPSDTAQLDRSKVIGFLTNIGGRTSHSAIMARTLEIPAVVGLGDITTSVKNGDTVIV
                     DGIEGVAIINPDEATINEYKARLEKFKAEQEELKKLIDVKTTTKSGRRIEVCGNIGKP
                     EDIDQVLANGGDGVGLFRTEFLYMDRDEAPTEDEQFEAYKYVLEKADGKQVVIRTLDI
                     GGDKTLPYLPLPEEMNPFLGYRAIRLCLDRKDIFRVQIRALLRASVYGNLAVMFPMIS
                     GLEEFQQAKAFVEECKAELKAEGIAYSDSIQWGIMVEIPAAAVYADELAKHVDFFSIG
                     TNDLIQYTLAADRMSEKVSYLYNPMHPAVLRLIKMTIDGAHKHGKWVGMCGEMAGDER
                     AIPTLVEYGLDEFSMSATSILTAKKIIMEQE"
     gene            16954..18960
                     /locus_tag="K8M77_000831"
     CDS             16954..18960
                     /locus_tag="K8M77_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBI7085345.1"
                     /translation="MYSRELIIKTPLGLHTRYSAMIVNKASEIESKYKVKLYIKKESY
                     TDWLGISMLAILSLKVLPNESILIGSKNEGMIEKLAVQSLLEFIDKNINNPIESEDSE
                     IDEIIDASIVANEQVLESLPIGIVVIDIDQNIITINQYALRFIGCNKKDVKGKKINEV
                     IPSSQLPHVMLSNLKKYGSTLHINNRVGLVNSSPLFINDKIIGAISVIQDVSDIIGMK
                     EINEKFTKILENSQDMICFVDENGIINYLNPAYIKNFSKVSSDVIGKSIFDIAPNGLR
                     AKVFKEKTLLKDVIHKKNGINVISTIDPLFIDGQFKGVISTSRPVSLIKELMSKLNKS
                     EQELDYYKNEFLRQLSKNSSFNNIIGSTRTLKDIMYMCQKASETTSTVLIRGESGTGK
                     ELIAKAIHNNSNRKNKPFVRVNCASIPENLLESELFGYEKGAFTGAVQSKPGKFAIAD
                     TGTIFLDEIGDMPLSMQVKLLRVLQEREIESVGGITPKNIDVRVIAATNRNLEEMIED
                     GSFREDLYYRLNVLGINLPPLRERKEDIPELAEHFITKLNKKLHKTILGIKQDALNLL
                     IEYSWPGNIRELENIMERAINLCDGDYIDSSYLPSYLKPVESKSFNLNIDINHILPFE
                     EYEKQIIEAAMKKYKSFNKAGKALGLTHRTVSLKCKKYNIEVKK"
     gene            complement(19133..19726)
                     /gene="rpsD"
                     /locus_tag="K8M77_000832"
     CDS             complement(19133..19726)
                     /gene="rpsD"
                     /locus_tag="K8M77_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBI7085346.1"
                     /translation="MAKMMGPRFKMCRRLGLNVVGHPKAMKRADRGTSRADKNLSDYG
                     IQLLEKQRLRAYYGVMERQFTRYVDQAFNSKEQPGEALLMILESRLDNMVYRMGFASS
                     IRQARQMVNHGHFLVNGKKVNIPSFRLNIGDEVVLREKSRKTEMFVNNFKDSIGSEVP
                     YISKEEDNFKGIFARKPKREEIPITIQEQLIVEFYSK"
     gene            complement(20127..21161)
                     /locus_tag="K8M77_000833"
     CDS             complement(20127..21161)
                     /locus_tag="K8M77_000833"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBI7085347.1"
                     /translation="MEINKRPLFNEFGDIETNKKKMINGNTTNLNDFNNMKYTWVSEW
                     YRQAMNNFWIPEEINLAQDLKDYKKLTKEEKAAYDKILSFLIFLDSIQTANLGNINSY
                     ITASEVNLCLTIQAFQEAVHSQSYSYMLDSICSPEERNEILFQWKDDAILLQRNKFIG
                     DLYNNFLEDSSIENFIKSVMANYILEGVYFYSGFMFFYNLERNGKMPGSAQEIRYINR
                     DENTHLWLFRSIIKELKEEIPEVFTKELKEELREMMRTGVEHEIAWGHYVIGDNVTGI
                     NKNLIERYIKYIGNLRAKAIGLEPLFEGYNENPAPWVDYYADANQVKTDFFEAKSTAY
                     AKAGALIDDL"
     gene            complement(21257..23488)
                     /locus_tag="K8M77_000834"
     CDS             complement(21257..23488)
                     /locus_tag="K8M77_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBI7085348.1"
                     /translation="MTLLERYCAWSIEELNDETGLYTEEKLMVALDNIVTSKMEEKEI
                     IKSFIQVSLELTSQVEPRWQEIAAKLYVKNLYEEVRTNRGLEEGANPYDNFYGFIKEL
                     TDKGLYGKYILDNYSKEDIEELEKEIKRERDFLFTYSGINLLAKRYLVQDFNRLPLEL
                     PQQMFMGIAMHLAIPEKKEKRLYWAKRFYDVLSSIKATMATPTMSNARKPFYQLSSCF
                     IDTVDDSLAGIYKSLDNFSKVSKFGGGMGIYMGKVRALGSAIRGFKGASGGIIPWIKL
                     FNDTAIAVDQLGVRNGSVAIWLDAWHKDLPEFLQIKTNNGDDRKKAHDIFPGLCYPDL
                     FWKLAENDIDANWYMMCPHEIKTVKGYSLEDFYGEEWEKKYYECVEDDRIEKRVMSVK
                     DIVRLIIKSAAETGTPFAFYRDTVNKFNPNKHKGMIYSSNLCTEIMQNMSAMEIEQTE
                     IKDENGDVVVIEKTKPGDFVVCNLSSVVLGNVDVTSDEEVEYVVETQIRAMDNVIDLN
                     YYSVPFAEITNKKYRAVGLGTSGYHHMLVNNKIVWDSEEHLNFVDKVYERINFYAIKA
                     SLNIAKEKGSYPLFEGSDWSNGDYFELREYNSPQWKELKEEIAKYGMRNGYLFAVAPN
                     GSTATLAGTSEGVDPVMNRFWLEEKKGSITPKVAPGLCQENFWYYVSAYNVNQEFTVR
                     ANGVRQRHIDQGQSFNLYITTDYTMRQIMNLYISACKNGVKSIYYVRNKSLSVDECES
                     CSA"
BASE COUNT         7537 a         4320 c         2708 g         9250 t
ORIGIN      
        1 atttctattt aaactttata tttgataaaa gttaactata aaacaaaaac cgtatcaaaa
       61 ttaatttaat cctttccttt gatacggttt cctactatca taaatatact tttaattata
      121 ttacttactt atttatttct ttaccacgcc atattaagaa agctatttta aatattacag
      181 cttcattaaa attccttata tcataagatg tacacttctg aattttatct aatctgtaga
      241 tgagagtatt tctatgtaca tataattcct tagatgcatc acttatatta agtccacact
      301 taaaaaatgt ttctatagtc tttatcatct ctctatccaa cttagaaaat cctaacccaa
      361 aatcttcata catcttcatc tttaagtcct catctatgga atcaactaaa gtttcaaaaa
      421 gtaaatctct ttctccataa atgacttcat ctaaatcata ttttattgca agatttatct
      481 tagacatatt tatctcataa gcctctttta tttcatttat attatttaat ttcatatagc
      541 ttatataaca ttttacataa aggtttgagt caatagaatc ctttatacta ttaacgtgat
      601 catcaacatc ttctaaagca ccaataatta ctatgttatc ctcttttaaa atgctaacta
      661 tatttttatt accatagcat tcattaacaa gttctaatcc ttcttgaagg tttgaagcta
      721 aataaacatt aattatatag aaatcacctt ttaaaaatcc taattcctta tgaataatat
      781 ttttgtctat agtaactcca tttaaagcct ttctaataag tttttccttg ttacttgaaa
      841 cttctttaat cttattttca attaaatatc ttaataaaga agtacagctt tcatattcct
      901 tttgagttat tatttttccc tttttaccat taacctctaa ttctaaagaa acattatcat
      961 aattatcaaa atctgatagt acaaatttct tgtcatccaa aacaactata aaaggtattt
     1021 tagtatttaa atataactct tctaagaact tatgaaaact gtacatatta attacccctc
     1081 tatattaaag ctattgctga ttaaactaat tttaattcag tttctttatc gaatatatga
     1141 atcttattag catcaacgta gaacttagct tcttgaccat tttgtaactt agtgcttcca
     1201 tttaccctaa ctgttatgtt ttggtcccca agtttagtgt agatataact ttctgctccc
     1261 attaactctg taacttcaac ttttgatttt attactgttg ttggatttgc tgcaactaat
     1321 tcttggtcat catctaaatg ttccggtctt atacctatta taacattttt tcctatatat
     1381 cctttttctt ttaataatct tgctttatcc attggcattt caattttttc actttggaag
     1441 caagcaaaat attttccgcc ttcttctttt atagtaccat ctataaagtt catttgtgga
     1501 cttcctataa atcctgcaac gaatatatta gctgggttat tgtatatttc ttgtggtgag
     1561 tcaacttgtt gaactatacc atctttcata acaactattc ttgtacccat tgtcatagct
     1621 tcaacttggt catgtgtaac atatataaat gttgtttgta atctttgatg taatttagag
     1681 atctcagttc tcatttgaac tcttaattta gcatctaagt ttgatagagg ctcgtccatt
     1741 aagaatactt taggttctct tactatagct cttcctaagg caaccctttg tctttgacct
     1801 cctgataaag cctttggttt tctatctaat aaatgctcta tatcaagtat tttagcagca
     1861 tctttaacct ttttatctat ttcatccttt ggaacttttc ttaatttaag tccaaatgcc
     1921 atgttatcat atacagtcat atgtggatat agtgcgtaac tttggaaaac catcgctata
     1981 tctctttcct ttggttcaac ttcgttaact aatctatctc caatgtataa ttcacctttt
     2041 gatatttctt ctaatccagc aatcattctt aaagtagtag acttaccaca ccctgatggt
     2101 ccaacgaata ctataaactc tttgtcagct atttctaagt taaagtcctt aactgctgta
     2161 acatctcctg gataaacttt ataaatatgt ttaagtgata aatttgccat attaataacc
     2221 ccccataaat ttaaattatt ttctcgaatt ccttttataa ttttatttta tacccattaa
     2281 ccgttttcat ccatgatcat tatttacaaa agaattcctt attttttgta tgataatcta
     2341 aataaagtgt ataaatttta tttttattgt gatattttta aataaaaaaa caggatgcat
     2401 taaacatcct gtctattttt taaatattat tttttatatt tcatgtgtaa taactcatga
     2461 gctcttccat gacctggttt acccatgtag ttttcataca tttttaataa agctgagttt
     2521 tgatgtgatt ttctcttctc taaattctta tcttgattgt aaagaacaga agctcttact
     2581 tctctaatat caattttatc actatcttca gctgagatat gtggttgtcc cccaccgtta
     2641 acacatccac ctggacatgc cattacttcg atgaagtggt aatcatttat tttaccagac
     2701 ttaactaatt caaatacatt agcagctcca tttacaacag ctaatttata ttcatttcct
     2761 gctatttcta cttcagcttc ttttattcca gctaatcctc taacagcttc ataatctaca
     2821 ttgtctaagt tatcattttc catgaaatct ttagctgttc ttaaagcagc ttccataact
     2881 ccaccagtag ctccaaatat aacacctgct ccagtatact cacccatagc tggatctact
     2941 tcactgtctt ctaatttagc aaaatctatt ttagcagcct ttatcattct agctaactct
     3001 ctagttgtta taactgcatc tatatttctt attccttcgt tttccatttc aggtctatca
     3061 gcttcgaatt ttttagaagt acaaggcatt acagttactg taaatacttt tttaggatct
     3121 atatcagcaa cttgaggata ataagttttt gatgctgaac caaatatttg ttgtggtgac
     3181 ttagctgttg aaagattttc tactaattct gggaagtagt tttcaacttc tctaacccat
     3241 gatgggcaac atgaagttaa cattgggaaa ggcccattat tttttattct ttctataagc
     3301 tcagtagctt cttccattat tgtcatatca gcaccgaagt ttatatcaaa tactttatca
     3361 aaacctagtt ttcttaaagc agtatataat tttccagtaa catcttggcc gtaacccatt
     3421 ttgaataatt cacccattga tgttcttaca gctggtgcta tagcaactat tacatgcttt
     3481 tctggatcat ttaatgcatc ttgaactctt tcaatatgtg atttttctga taatgcatca
     3541 actggacaag cagctacaca ttgtccacat aataaacaat ttgtatcgtc gaaacatttt
     3601 aattcctctg gtcctactaa tcttactcca tcaattctgt ggaattttat tgagttagta
     3661 gcagttcttg ttctacatgc agcaacacat ctaccacatt taacacattt acttctatct
     3721 aaaactattg atttacttct gtcatctaca tattctgatt tatctgcaac tacaaatggt
     3781 ttgtgagctc ttgcctttgt tttaattaca agttttaaga attcacaatt ctctcttctt
     3841 ttacatggtc cacatttgaa ctcatgctca tctaaaagtt ctgcaactct ttcttttcta
     3901 gcttctagta ctttttcact atcagtttta ataaccattc catctttagc ttttattgca
     3961 caagatctta atagtctatc atttccttct tgctcaacaa gacaaactcc acattgtcct
     4021 ttgtttccgc aattttttaa ctcacaaagc actggaatgt caaacccatt ctctcttgct
     4081 aagtcaagta tcgtcttgtc accatcaaat tcgatagtct tatcattgat tattatttta
     4141 ttcatgctgt tcctccaatt tttggttttt tacttaatac taaacctcaa ctcatacatt
     4201 gtaaaaatca gttaatttaa aaatcgtcgt tattattcta acataatatt tgtgaaatat
     4261 aaatatttca gatttttatg aacacttttt taaaacaaaa tttaagggag aattacaaaa
     4321 ttcttccctt aaacaatcta aactattcaa atttatatat attaattaat ctatttatat
     4381 atctttgctt cttcaactcc atctaaaact cttatagctc cttgtgctaa agctaaaagt
     4441 tcatcttctc caccgtatac aaccataggt gcaatccatt ttactttttt atttatagca
     4501 ttagttacat aatcactata tgcaattcca ccagttaata ctatagcatt aacttctcca
     4561 tctaaaacta cagccattga tcctatttct tttgctattt gatatatgaa agcattaaat
     4621 attaatgagc ctttttcatc accctcttgt gatagcttta ttaaatctct tgcatcattt
     4681 gtgttagcat aagcaacaaa tccgcctttt ccaactaact ttttataaac ttcttctttg
     4741 ctatactttc ctgagaaaca catttctaat aattcacctg aaggaactcc acctgctctt
     4801 tctggtgaaa atggtccatc accgtctaat gcattattta catctataac tctacctttt
     4861 ctatgtgctc ctactgaaac tccgcccccc atatgaacga ctattaaatt aacatcttca
     4921 tatgaagtat tatgttcttt tgcatatctc ttagcaactg ccttttgatt taatgcatgg
     4981 aatttacttt ttctaggtaa ttctggaact cctgataatc ttgctacatc ttccatttca
     5041 tcaacaacaa ctggatctac tataaatgct ctcttaccaa tttcttttgc tatttcatta
     5101 gataatattc cacctaaatt tgaagcatgt ggtccttgaa ccccaatttt taagtcctca
     5161 accatagctt cattgacttc ataagttcca ccttctattg gcttaagcat tccacctctt
     5221 ccaacaacag catctaactc atttatgtca ataccttttt cttttaatac ctttaaaata
     5281 acttcttttc taaaatcaag ttgatcaaat attgtatcat attttagtat ttcttctgct
     5341 gaatgtctta aagtttcttc taagatttct ttttcacctt cataaacacc aattttagtt
     5401 gatgtagacc ctggatttat tattaataat ttatatgcca tataactttc ctcccccaaa
     5461 attctctact ttaaatcatg agcaaccact gctgctatag ctattgattt aacttttgtt
     5521 tcaaaactat ctgctctaga agttaatata actggagctg atgtaccaac taataatcca
     5581 ccattcttac attctgacat gtaagttaaa gtcttgtaca ttacatttgc aacatctata
     5641 tttggtaata ataatatatc tgcttttcct gcaacttctc cttttacccc tttatgctct
     5701 gcagcttcct ctgatatagc attatctaag gcaaatggtc catcaactat acatcccttt
     5761 atttgtcctc tatcattcat cttagctaat aatgctgcgt ctattgttgc tggcatatta
     5821 ggatttacaa cctctaccgc ccctataaca gctaccttag gaacttcaac tccacaagca
     5881 tgtgcaacct taactgagtt atttatcatt gataccttat cttttaactc tggataagta
     5941 ttaaaagctg catctgttaa gaaaagtaat ctatccattc ctggtatttc aaatacagat
     6001 acatgagaca ttaattgacc tgttcttaag ccaacttctt tatttaagac acttcttaaa
     6061 aaagtagcag tatcaactag acccttcatt accatatcag tttttcctgt ggatgctagt
     6121 tctactgcta ataatgctgc ttttttatcg ttaggttcat ttataatttc aaagtcagct
     6181 aaattcatac ctattttctt agctatttct tttatttttt cttcattacc aactaaaaca
     6241 gcctcagcta ttcctaattc ttttgcttct cttacagcct ctaaaactgg ttcatcttga
     6301 gcaactgcaa cagaaacttt tttcatacta ttttctctta gttttgaaaa caattcatta
     6361 aaatttttag ccataatact caaccctctt ccttgaattt tgttaaattt ataacaattg
     6421 taaacaaaaa aaaactgact atataaattt atcaattcaa aattaattct ccataactat
     6481 attctaccaa aaaatattct gaaaacatta gttttcagaa tattcaattt attatttttt
     6541 attaaattgt taaattttaa acatgatatt ctttcattat attttcagct acttttaatc
     6601 catctacagc tgctgaaaca ataccgcctg caaagccagc accctctcct gctggatata
     6661 aaccctctaa agatatactt tgtaactttt catttctttc aattctaact ggagctgatg
     6721 ttcttgtttc tataccagtt aatacactat cactatgtcc aaatcccttt atctttctat
     6781 caaaatcagt tattccctct tttaatccat ctattacgta agaaggtaaa cactttctta
     6841 aatcttcaaa tctatatccg ttgcttaata cacttggatt aacttttcct aacttagttg
     6901 taactctatc tttcataaaa tctccaacta attgaactgg agtgttataa ttttctccac
     6961 ctagtttaaa ggcaagttct tcatagtgtc tttggaactt cattccttct aaaggatgat
     7021 ctcctccaaa gtcatttgga cctacagtta caactagagc agagttagca ttttctttat
     7081 ctctagcatg atagctcatt ccatttacgc ataatctttt ttcctctgaa gaagcattta
     7141 caactacgcc tcctggacac atacagaatg aataaactgc tctatctaaa gttttacttt
     7201 gatatgctaa tctatattca gcagccttta atcttggatg gttagcatat ttttctccat
     7261 actgacttaa atttataatt tcttgtggat gctctattct aactccaata gcaaagggtt
     7321 tttgcttcat aaatacacct tcattaaata acatttcata tgtatcccta gaactatgtc
     7381 ctaaagctaa aattgctact tcacaaggta cttcttctcc atttactttt atccctttta
     7441 atttgttatc tttttttatt attccttcaa accttgaatt gaaatgaact tctccaccat
     7501 ttctttttat ttcttctctt atattcttaa ctacattttt taatatatca gttcctacat
     7561 gaggttttcc tttatatatt atttcctctg gtgctccatt tctcacaagg gcatcaagaa
     7621 cataatcaca tcttgtatct tttattctag ttgtaagttt tccatctgaa aatgctcctg
     7681 ctccaccttc accaaactgt acatttgact ctggatttaa ttctccagtt ctccaaaatt
     7741 cctctacagt tttagttcta gaatctacat cttctcctct ttcaaagaca attggattaa
     7801 atcccttctt agctaataaa agagctgcaa aaagccctgc tggccctaat cctacaacta
     7861 ctggtctgtg ctgaagtttt ttctccccaa aagtaaaatc aaaatcataa tttaccttat
     7921 ctatctttac atccttatca ttaagtctag ctactatttt ttcttcatct tcatgttcta
     7981 tgtttataca gtaattaaac tttataagat tcttttttct tgcatctaaa gattctttta
     8041 ttatttttat atctccaatt tcatccttag acatccttag ttttttagct actttatttt
     8101 ttaaaagact taaatcttca tttatatcta aactaatgtt atttattcta atagtcataa
     8161 aacttcctcc attttatttc tttagtaaat tattttacca cagaatacgc ttaaagtaat
     8221 ctgtggtaaa tatttattgt ttattgtttg agttttcatt attacttccc tttgaagtat
     8281 tattatttga attatcactt ggttttgtag aattattctc tgtactttct gttgaattag
     8341 tattcttttt aaaggttacc ttaacctctg taggatttat actaacaacc tttatattat
     8401 cgtaagagtt tgatatatta ggctttacac ttcctccatc agctgtaagc tttgatatat
     8461 ctatagtaca tttaaattta cttaaatcaa tactatttac tgaatcttca ggaccattaa
     8521 ttgttacttt tatagtttta gtatctaatt ctggagtata tccatctaaa gtaccctctg
     8581 tagttactgg tacttctatt tccttttgag catctttact aaaatctaat ttaactttaa
     8641 tttgagcttg atcattagag gctaaactta ctccatctgg aatttttaat actgttgata
     8701 cctccgaatc atctccatac tgtgaaacat ctattggtga tgtctcaata gagctaatct
     8761 tacttaaagc atcctctgat ccaagtaaag tcacctttgc aatacttggt gttattgatt
     8821 ttaaggctag cccatcctta ggagtgccag tcgtaattac actaactggt acttccttag
     8881 atttcttaac tccaaaggat acatctactt tattaggact tatattaaca tccttaattt
     8941 cgttgccatc tccatctact gccacaacag gcaaattcat attaactgcc ttactaacat
     9001 cgtttatctc accctttact tctaaagtct taactttact tactaatgat tgagctccag
     9061 atacagtggc attttgtggg cttatattta ttttatctgc ataagtacca tgctctgtat
     9121 ttacctttat tttgctaaca ataggcaaac ttttttcttg gtatttatca agaataacat
     9181 ttacccttag aaatccattg ttttttatgt ttatattatt aggataactt tctatttgta
     9241 ctggtatatt attgtctccc tcttttaaaa catactcact taaattaacg accaccttaa
     9301 attgttcttt tttgacctta tatatttcag tagatggccc ttctaaatct aaatccacag
     9361 tcagattttg attaggaacc attactaaat cataatcctt aagtacttca ctattaacaa
     9421 cctgaactgg cacgttattt aatttaagct ctctcactgg attttctaca tttattatat
     9481 atagccaaag agaaaaggat aataataagc atataaattt tactaagaaa ttctttcctt
     9541 caattttttt aagcatattt tcaccttctc tccataagtt ttccctctct tatcacgtct
     9601 atttctcata acttttaata atatattttt taatttttta ccatcataat ttcttgttaa
     9661 tctacctttt acagctaaag atatagctcc tgtttcttct gaaacaacaa taactaaagc
     9721 atctgatatt tcagataacc ctatagcagc tctatgtcta gtaccaagtt ttttatttat
     9781 agtattatta tttgttaatg gtaagacaca acctgctgaa acgattctat catttctaat
     9841 tatagttgct ccatcatgta atggagtgtt cttaaagaat atattctcta ttaatgcaga
     9901 agttattact gcatctaatt gggttcccgt agaaattatt tctgctaatc ctgtcccttg
     9961 ctctatagct attaaggcac cagttttagt ttcagccata tcctctactg ctactataag
    10021 ttgatcaata acttcctcta atttttgatc atcttgaata acatgaaaat catcaaaagc
    10081 agtacttcct atatgttcta atgctcttct tatttctggt tggaaaataa taattattga
    10141 taaaacacct atggtcaatg tttttgttaa tataaaattt aacatttgaa gtcttaaaac
    10201 atagcttata ggaattaaaa tcaataataa tattactccc tttaataatt gctctgccct
    10261 tgtttctttt attaatatat atcccctata aaatataaaa gctacaatta atatatctat
    10321 gatggaccaa atagatatat cctttaaact atttgtaaat atcattatta attcttgcac
    10381 aaatttcacc tccattaccg taacaattaa attatacaat aaaaatctaa ttataagata
    10441 cattttaaat gatttctttt cagttttaca tattctaggt aatgttatta tacattctct
    10501 catatatttt aatagaaaat tatataaggg ggtacttgct atggacttta aatataaaaa
    10561 atatattgat aattccttaa tatatataat atttgcagtt cttatattaa tatttataat
    10621 aggtttttta tttttcagat cacataatga aaaatctaaa cttgaagacc taggaagaac
    10681 tatttctgaa attaatctta cttatgattt ttctgaagat agtataacat ctgaagatta
    10741 tgttagttct atagaaaata aattaaagaa acttcaagaa acaaattcgg ctttaaaatc
    10801 tttaaaagtt tctcaaaagc atcaaaattt atcctctcca cttaaggatg gattagataa
    10861 aaatatagaa ttgtttaata aattactttc tatattgaaa actccagatg ctttaaatat
    10921 atctgatgaa tacgctattg cttcaaaatt aaagaaagaa tgtgaaaatt attattctat
    10981 atgtaggtct aacttaatac ctgtatatct atataaagag ggaaataatt atctacagga
    11041 tattttttat tatataaatg agctaataaa aactaataga gataaaggct ttgtccaaag
    11101 ccaaaagaat gattttgttg tttctatgaa atcattactt cttaaacttt cttcaatgga
    11161 tgaaaaactt tttgaaacag ctaacctaat aaaagaatca aatagggatt taaaggtttt
    11221 agttacagat atagaaaata aactttcttc tcttcaagag ttaaaaaata atttatattc
    11281 cttacctatt cctgaacaag ctaacgattc tttcttagct cttgaaaaag cactaaaatc
    11341 ctatgataag tatattaatt atttctacaa aggcctttta gatgaaattg aaaataaaaa
    11401 gacaccagaa gattattatg ataaatcctc aactttattt gatgagttta tctattcctt
    11461 agaagctgct gaaaagtctt tagataacta taataaaaat taaataggag tattaattct
    11521 atttttttgg ttacaatata caaagtaaaa taattaccta aaaggaggtt cttatgaaaa
    11581 aaagtattta taattacttt atcttcacct tcgccttatt aattatcact acacttaatg
    11641 agaaagaagt tttagggctt tctctatcaa atgagcatgt aaccaaaaat aattatatag
    11701 aattaacaga aatggcttct aattcgccaa atcctgaagc agaacaaagt agtaattcaa
    11761 ataatgaagt tattgctgta ttccaaagtt caaactctac aatttcactt actaaagatg
    11821 acatatattt aatgtctcaa gtagtttatg ctgaaagtaa aggtgaacct tttgatggaa
    11881 aaatagccgt tgcatcagta atacttaata gaacaacgga ttctcaattt ccagatacaa
    11941 ttcatggagt aataacacag aaaaatgcct tttcttgtgt tagaaatggc aaaattgacg
    12001 tagtccctga tggtgatagc tataatgccg ttctaaaagc catagaagga tatgacccaa
    12061 cagatgaggc attatatttt tataacccta aaatcgctac atgctcgtgg atgaaaggcg
    12121 tcgaaaagac aggagaaaaa attataggtc aacatgtatt ttttaatgtt acctaaagaa
    12181 aaagctaaca caatgtgtta gcttttttta tttcttactg tctaagtatg ctactgcact
    12241 taaagcagcc acatttcctt cacctgcagc cttcatatat tggtatggtc taccagtaca
    12301 atctccagca gcaaaacagc cttctatatt agtcttcatt aatctatcaa cttctatgtg
    12361 atcatcagtt atttttaatc ctggcactaa ttgtcctggt gatatgctat ctcttaatac
    12421 aaatatacca tcagtctcta tttcaccgtt tttagttact aatttagtaa ccttgctatc
    12481 tccttgtatt tctacaggaa tatcattaat aacttcaatc tctgaattta aatcaaattc
    12541 tcccttatat ctaggaatat agtaaacctt agctgctaat tcacttacaa aattagcttc
    12601 ttcttcagct tctttattat agccaattat agttacagtt ttttctttat ataaaggggc
    12661 atcacaagta gcacagtatc ctacaccttt acctaagaac tcttcttcac ccttcatagg
    12721 ttttgtgtat tcaatacctg ttgctatgat tattgaagtg gcttcataca tcttttcatt
    12781 taccataaga gcgaaatatt ctcccatagc ataaatacta tttattcttt cctctgttat
    12841 ctctatatcc atctctttta gatgacccat aaattgattc ttaagatcca taccatcttt
    12901 tccaacgaat ccaagatagt tatttacctt tggagcttta acaagctttg tacttaaatc
    12961 attgttacca aaaagaataa attttttatt tctaattttt gcatttaatg cagctgaaag
    13021 gccagccggt ccgcttccta ttatagctat atcatatctt tcgctcaaaa ttattcatct
    13081 ccttaagaag cttattaaac gtgttttttg atagcttcaa ctaatgcagc ttttggtcta
    13141 aaaccaacta aagtatcaac aacttttcca tctttaaata ttaataaagt tggtatgctt
    13201 tgaatttgat attttccagc tgaacctggg tttttatcaa catcaacctt tacaaattta
    13261 acattttgca tttcatttgc taattcttct ataactggtg ctatcatttt acaaggtcca
    13321 caccaagttg cccaaaaatc aacaactact acttcacttg cttctattac ttctgtatta
    13381 aaatcttgat catttatatg ttttaccatt atatacgcct cccgatatac ccctaagggg
    13441 tactttatta tttacaatta tattatattt aaaaaattaa gtcaacgtta caaataataa
    13501 ttattatcta atttttataa ttttgttatt caaaggtgcc ttttactaat aattttttat
    13561 tatccattaa aatattacct ctagatataa aagaatttat gtctaaattt ttcttatcta
    13621 ataaaattat atctgcatct tttcctgttg atataaatcc tttatgagat agttttaaaa
    13681 cattggcaac attactagtt ataactctta ttgctttttc taaaggtact ccaagcttta
    13741 tagcttcctt tacttcatta aaaagacttt caacagaaca aattcctaaa cctttaagct
    13801 ctccattttc atcaaataat ggcatacttc cattaccatc tgaagaaaaa gttattcttt
    13861 ctatatctac tccagcatct aaaagctttt taagtccttc gccagctctt acttctcctt
    13921 cttctaaatg ttgaggatct gaagatgtag ttaaatcaat ttggcctcca gccttagcat
    13981 gatttattcc tgcttcaaaa agctctctac ttcttccaat atgagtagga attatttgat
    14041 ttgctggaat ctctgtttca tttataagtc tagttatata ttcaatcatt cttttcccat
    14101 tacctaaatg aacatttact attccagcct ttcccgataa aagtcctcca actcttgctt
    14161 gagcaactat attgacgaat tgctcgtatg ttggttgaga acttctatgg tctgatagtg
    14221 ctatttcccc tacacctata actttatcta ttaataataa gtcacttttt atggttttag
    14281 ttatagtatt tacaggcaca tcgtaagacc cagtataaca gtaacaagtt actccctctt
    14341 cacaaagagc atgagctttt gcaactagtc ctttcatatc tctacaaaca ccatcagttc
    14401 ctatacaccc tactagggta gttattccac cttttattat actacttaat tgtaattctg
    14461 gagttctggt tctaaaacca ccttcaccac ctccaccaat tatatggaca tgacaatcta
    14521 taaagcctgg tacagctatc atcccttctc catcaattac ttgtatgttt ataaaatctt
    14581 taggaatatt cacagaatca tataatcctt cgatcttatc acttaatata acaatatcct
    14641 ttttccctaa atactcaggg tcataaacct caatattttt tattactttt aacaaaaaat
    14701 acacctcctt tatttaaatt attattattt ttacctaaaa ctcaataata aataatttaa
    14761 ttttataaaa acttattttt ttataaaaaa aaggcacgaa ttaattcgtg ccaaaactta
    14821 gggttttgag tgtcctatat taatttttaa tattattgac tattcttgtt ccattattat
    14881 tttcttagca gttaggattg atgtagcact cattgagaat tcatctaaac catattcaac
    14941 taatgttggt atagctcttt catctcctgc catctctcca cacattccta cccacttacc
    15001 atgtttgtga gctccatcta ttgtcatttt gattaatctt aacacagctg gatgcattgg
    15061 attgtaaagg tatgatacct tttcactcat tctgtcagca gctaatgtat attggattaa
    15121 atcgttagtt cctattgaga agaaatcaac atgcttagct aattcatcag cataaactgc
    15181 tgcagctggg atttcaacca tgatacccca ttgaattgaa tctgagtatg ctataccttc
    15241 tgcttttaac tcagctttgc attcttcaac aaatgcttta gcttgttgga attcttctaa
    15301 tcctgaaatc attgggaaca ttactgcaag atttccataa acagaagctc ttaataaagc
    15361 tcttatttga actctaaaga tatcttttct gtctaagcat aatcttatag ctctgtatcc
    15421 taagaatgga ttcatctctt ctggtaatgg taagtatgga agagttttat ctccaccgat
    15481 atctaatgtt ctgataacaa cttgcttacc atctgctttt tctaaaacat atttgtatgc
    15541 ttcaaattgt tcatcttcag ttggagcttc atctctgtcc atgtataaga actcagttct
    15601 aaatagtcca actccgtctc caccatttgc taaaacttga tctatatctt ctggtttacc
    15661 tatgtttcca caaacctcta ttcttctacc tgatttagta gttgttttaa catctattaa
    15721 tttctttaat tcttcttgtt ctgctttaaa tttttctaat ctagctttat attcatttat
    15781 agtagcttca tctgggttga ttatagctac accctcaata ccatcgacta ttacagtgtc
    15841 cccattttta actgaagttg ttatatcacc taatccaaca acagctggta tttctaaagt
    15901 tctagccatt atagctgagt gagaagttct tcccccgata ttagttaaga aaccaataac
    15961 tttacttcta tctaattgag cagtatctga aggtgttaag tcatgagcta caacaacagt
    16021 gttagctcct acattttcca ttccgtttcc acccttgcca gctaggttag ctattattct
    16081 cttagaaacg tcttttatat ctgctgccct ttctctcatg taagggtcat ccatagaatc
    16141 aaatatcata acgaaagtat ctgtaacatt ttgaacagct ttcatagcgt ttaattggtc
    16201 attttctata gtcatttcca tttgaccagt aaactctaca tcatctaata acattaaatg
    16261 ggcttcaaaa acttgagctt catgttctcc aatttcatta gctgtttttt cttttattgc
    16321 ttctaattgc tcttttgatt gagctaaagc tttagataat ttttcttttt cagctgctat
    16381 atctgaaacc ttcgcatcag atattattat ttcctcatgt tcttgtataa atacagttcc
    16441 tattgcatat ccttttgaag cagcaatacc ttttttcatt atgtagtcct cctttaagtt
    16501 tgtactcaac taaatacaac attaaatatc aatcacaatt caactaaatt cacttttcaa
    16561 attcactgct tatatataac agcaatttgc gtgccaagtt tttaattatt aatatttttc
    16621 aaaatatatt atactcataa accctagtaa aaatgcctat aaatcttata aaaatatttt
    16681 atttattaaa aacttacgtt aaaatactca gtcatattag ttgtttttat tatactaaat
    16741 gtgataaaaa atatcaaact aattttacca ttgatatttt ttatcaaatt ttctttttaa
    16801 aataatcttt ttattaagat atatatacat aaagccttta aaatagtatt tttttctgat
    16861 aatatattat taatacttga taaaaaatat caaccttatt cactttttat ataaattcat
    16921 aaaaaaataa aactttggac ggtgatttac tccatgtatt caagagaatt gattataaag
    16981 acgcccttag gattacacac tagatactct gctatgattg ttaataaggc ttctgaaata
    17041 gagtctaagt ataaagttaa actctatata aaaaaagaat cttatacaga ttggcttgga
    17101 ataagtatgc ttgcaatact ttctcttaag gtgttgccta atgaaagtat attaattgga
    17161 tctaaaaatg aaggaatgat tgaaaagtta gctgttcaat ctcttcttga gtttattgat
    17221 aaaaacataa ataaccctat tgaatctgaa gatagtgaaa tagatgaaat aatagatgct
    17281 agcatagtag ctaatgagca ggttcttgaa agtttaccaa taggtattgt tgttatagat
    17341 atagatcaaa atataataac aattaatcaa tatgctttaa gatttattgg atgcaataaa
    17401 aaagatgtta aaggaaaaaa gattaatgaa gttattccat catctcagct tcctcatgtt
    17461 atgcttagta atttaaaaaa gtatggttca actcttcata taaataacag agttggctta
    17521 gtaaatagct ctcctctttt cataaacgat aagattatag gggctataag tgttattcaa
    17581 gatgtatctg atattatagg tatgaaagaa ataaatgaga aatttactaa gatacttgaa
    17641 aactcacagg atatgatttg ctttgtagat gaaaatggca taataaatta tttaaatcct
    17701 gcatatatta aaaacttttc taaagttagt tctgatgtta taggaaaaag tatttttgat
    17761 attgctccta atggacttag agctaaagta tttaaagaaa aaactttatt aaaagatgta
    17821 attcataaga aaaacggtat aaatgttata agtactattg atcctttatt tattgatggt
    17881 cagtttaaag gagttatttc tacttctaga ccagttagtt taattaaaga acttatgtca
    17941 aaactaaata agtctgaaca agaattagat tattataaaa atgagttttt aagacagtta
    18001 tctaaaaatt cttcttttaa taatattata ggttcaacta gaactctaaa ggatataatg
    18061 tacatgtgtc aaaaagcttc agaaactaca tcaactgttc ttattcgtgg tgaaagtggt
    18121 acaggtaagg aacttattgc aaaggctata cacaataata gtaatagaaa aaataagcct
    18181 tttgtaaggg ttaactgtgc ttcaattcct gaaaatctct tagaaagtga attatttgga
    18241 tatgaaaagg gagcttttac tggggcagtt caaagcaaac ctggtaaatt tgctattgct
    18301 gatacaggta ctatattctt agatgaaata ggtgatatgc ccttatcaat gcaagttaaa
    18361 ctccttagag ttttacaaga aagagagatt gaaagtgttg gaggaataac tcctaaaaac
    18421 attgatgtaa gagttattgc tgcaactaat agaaatttag aagaaatgat tgaagatggt
    18481 tcctttagag aggatcttta ttacagactt aatgtcttag gtattaatct tcctccactt
    18541 agagaacgta aagaagatat tcctgaactt gccgaacatt ttataactaa gctaaataaa
    18601 aaattacata aaactatatt aggaataaaa caagatgccc ttaatctact aattgagtat
    18661 tcttggcctg gaaatatacg tgagcttgaa aatataatgg aaagagcaat aaatctttgt
    18721 gatggagatt atatagatag ttcttatctg ccttcatatt taaagccagt tgaatcaaag
    18781 tcttttaatt taaatattga tattaatcat atacttcctt ttgaagaata tgaaaaacaa
    18841 ataatagaag cagctatgaa gaagtacaaa tcctttaata aggctggaaa agctttagga
    18901 ttaactcata gaacagtttc tttaaaatgt aaaaagtata atatagaggt aaaaaaataa
    18961 taaaagaaaa aaagcacttg gagaccagca catctccaag tgcttttttt attagtaaga
    19021 gtttagtaac attctgaagt ataattatga taagactttt ttagttttaa ctacgggaac
    19081 agctaataat taaaactaaa tttaatatta aataagttat acagtatttc tattatttag
    19141 aatagaattc aactattaat tgttcttgta ttgttattgg aatttcttct ctcttaggct
    19201 ttctagcgaa aatcccttta aagttatctt cttctttact tatgtaagga acttcacttc
    19261 ctatgctatc tttaaagttg tttacaaaca tttctgtttt tcttgatttt tctcttaaaa
    19321 caacttcatc acctatattt aatctgaaag atggtatatt aactttcttt ccatttacta
    19381 agaaatgtcc atggttaacc atttgtcttg cttgtcttat tgagctagca aaccccattc
    19441 tatataccat attatcaagt cttgattcta aaatcataag taaagcctca ccaggttgtt
    19501 ctttactatt gaaagcttga tctacgtatc tagtaaattg tctttccata acaccgtagt
    19561 aagctcttaa tctttgcttt tctaataatt gaataccgta atcagataaa ttcttatctg
    19621 ctctgcttgt tcctctatca gctcgtttca ttgcttttgg atgtccaact acatttaatc
    19681 caagtcttct gcacattttg aaacgaggtc ccatcatctt agccatactt aacctcctaa
    19741 tgttttttgt tttctatgat gctatattaa cataaaacct cattcattga aagtgattat
    19801 cattattgta tgattcgttt aattaaaatt atcttattaa atctttaaaa tactaactta
    19861 tgcttataaa tttaaactta tttattctat aaaataaatt catttataat aaactctaat
    19921 tttagtaact agattcaatt agctatcaat ctattccact taaatcactc ttatttatat
    19981 atgaatttta gtatagacat aaaaaaggct atatcaaact cttaacttat aagaattcat
    20041 aaattttata tttataaatt ctctcataaa tagtattgat atagctctct tttataattt
    20101 atgcagtcaa atattaaatt ctatttttat agatcgtcta ttaaagctcc agctttagca
    20161 tatgctgttg atttcgcctc aaagaagtct gtttttactt ggtttgcatc agcataataa
    20221 tctacccatg gtgctggatt ctcattgtag ccttcaaata aaggctctaa acctatagct
    20281 ttagctctta agtttcctat gtattttata tatctttcta taagattttt atttattcca
    20341 gtaacattat cacctataac atagtgtccc catgcaattt catgttcaac accagttctc
    20401 atcatttctc ttagttcttc ttttaactct ttagtgaata cttctggtat ttcttctttt
    20461 aattccttta ttatacttct aaataaccat aaatgagtat tttcatctct gtttatatat
    20521 ctaatctctt gagctgaacc aggcattttt ccatttctct ctaaattata gaaaaacata
    20581 aatcctgagt agaaataaac accctctaaa atatagttag ccattactga ttttataaag
    20641 ttttctatgc ttgagtcttc taagaagtta ttatataggt ctcctataaa tttatttctt
    20701 tgaagtaata tagcatcgtc tttccattgg aataatattt cgtttctctc ctctggtgaa
    20761 caaatactgt ctagcatata actatagctt tgtgagtgaa ccgcttcttg gaaagcttgt
    20821 atagttaaac ataagttaac ttctgaagca gtaatataac tatttatatt tcctaagtta
    20881 gctgtttgta ttgaatctaa aaatattaag aaagaaagaa tcttatcata tgccgctttt
    20941 tcttccttag ttaacttttt ataatctttt aagtcttgag ctaagtttat ttcctctggt
    21001 atccagaaat tattcattgc ttgtctgtac cattctgaaa cccaagtata tttcatatta
    21061 ttaaagtcat ttaaattagt tgtattaccg tttatcattt tctttttatt agtttcaata
    21121 tctccaaact cattaaaaag aggtctttta tttatttcca ttttacgtca cctttcatct
    21181 ctttatttat ctatctataa ttaatagata ctcacctaag cctagatgag tatctttaaa
    21241 tttcctaatt tatattctaa gctgaacagc tctcacattc gtcaacactt agagatttgt
    21301 ttcttacata ataaatgctt ttaactccat ttttacaagc tgaaatataa agattcatta
    21361 tttgtctcat agtataatct gtagttatat aaaggttaaa tgattgtcct tggtctatat
    21421 gtctttgtct tactccgttt gctctaactg taaattcttg atttacattg taagctgaaa
    21481 catagtacca aaaattttct tgacataatc caggtgctac ctttggagtt atgcttcctt
    21541 ttttctcttc taaccagaat ctattcataa cagggtctac cccttctgaa gttccagcta
    21601 aagtggcagt tgaaccattt ggagctactg caaataggta tccatttctc ataccatatt
    21661 tagcaatctc ttcttttaat tctttccact gtggtgaatt atattctctt agttcaaaat
    21721 aatcaccatt gctccaatct gatccttcaa ataatggata gcttcccttt tcctttgcta
    21781 tatttaaact agcttttata gcatagaagt ttattctctc ataaacttta tctacaaagt
    21841 tcaagtgttc ctctgagtcc catacaatct tattatttac aagcatatga tggtatcctg
    21901 atgttcctaa tccaacagct ctatatttct tgtttgttat ttcagcaaat ggaactgaat
    21961 aataatttaa atctattacg ttatccattg ctcttatttg agtttcaaca acgtattcca
    22021 cttcttcatc tgatgtaaca tcaacatttc ctaataccac tgaagaaaga ttacaaacaa
    22081 caaagtctcc tggcttagtt ttttcaataa ctactacatc accattttcg tccttaattt
    22141 ctgtttgttc tatctccata gcactcatgt tttgcattat ttcagtacaa agatttgatg
    22201 aatatatcat tcctttgtgt ttattaggat taaatttatt tactgtatct ctatagaaag
    22261 caaatggtgt tcctgtttct gcagcacttt ttattataag tcttacaata tccttaacac
    22321 tcataactct cttttctatt ctatcatctt ctacacactc ataatatttc ttttcccatt
    22381 cttccccata gaaatcttct aaagaatatc ctttaacagt ttttatttca tgaggacaca
    22441 tcatatacca gttagcatct atatcatttt ctgctaactt ccagaataaa tctggataac
    22501 aaagtcccgg gaatatatca tgagcttttt ttctatcgtc tccgttgttt gtttttattt
    22561 gaagaaactc tggtaaatct ttatgccatg catctaacca aatagcaact gatccgtttc
    22621 taactcctaa ttggtcaaca gctatggctg tatcattgaa taattttatc caaggtataa
    22681 ttccacctga tgcaccttta aaacctctta tggctgagcc taaggctctt accttaccca
    22741 tataaattcc cattccgcca ccaaacttag aaacctttga gaaattatct aatgatttat
    22801 atataccggc taaactatca tctacagtat ctataaaaca tgaacttaat tggtagaatg
    22861 gctttctagc attagacata gttggagtag ccattgtagc ctttattgaa cttaaaacat
    22921 cataaaatct ttttgcccaa taaagtcttt tttccttctt ctcagggata gcaaggtgca
    22981 tagcaattcc cataaacatt tgttgaggaa gttctaatgg taatctatta aaatcttgaa
    23041 ctaagtatct ttttgctaat aaatttattc cactgtatgt aaataaaaaa tctctctctc
    23101 tttttatttc tttttctaat tcttctatat cttccttaga gtaattatcc aaaatatatt
    23161 ttccgtataa ccctttatca gtaagttcct ttataaaacc ataaaaatta tcatatggat
    23221 ttgctccttc ttctaatcct ctatttgttc ttacttcttc atataaattt ttaacatata
    23281 acttcgccgc aatttcctgc catcttggtt ctacttgaga agttaactct aatgaaactt
    23341 gtatgaaaga ttttattatc tctttttctt ccatttttga cgtaactata ttatctaaag
    23401 caaccattaa cttttcttca gtatataatc ctgtttcatc attcagctct tctatagacc
    23461 atgcacaata tctctctaat agtgtcatta actagccctc cacaatatat agtaattatt
    23521 tttaaatttc gcacaatatc ttgacaattt attataataa tttaaagttg ttttgtaaaa
    23581 ctcagatttc ttgaaaaagt tagcttattc tatgaataac ccttattata taagtcatat
    23641 tttctttttt tagtcttatt tgactaataa ttattattga cattttaaaa ttacttctac
    23701 agcaagaata ataaagcttt taattaagta taaatgaaaa ggggctgtat caaaataaat
    23761 tttgatatta gtcgtgaaaa aggtggattc ataaatttga atccaccttt tttgc
//
LOCUS       DAEVGI010000022        23087 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEVGI010000022 DAEVGI010000000
VERSION     DAEVGI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 23087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23087
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.022"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            64..1614
                     /locus_tag="K8M77_000835"
     CDS             64..1614
                     /locus_tag="K8M77_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBI7085349.1"
                     /translation="MNTLNDILSKEIEEFREKGHKFLNKEMTVGEFRKVSGGMGVYAH
                     RGGKEFVVRLRIPSGVARLSSLEQVYNWAKKYNLEKIHLTTRESIQLHGISIDAVCDI
                     MQDGLTKGIYTRGAGGNFPRNVALNPLSGVQIGETFDVTPYALAANAHYMSKIYTYNL
                     PRKIKTAFSNTEEDSAHCTVTDLGFLAVENKGKRAFKLYIGGGLGRNPRKSVEFDELI
                     DEKDVLYCLEAITNLFVAEGDYKNNSKARIRYIVERMGEEEFKNCFRKHLEEVKAKGG
                     LDLKVEDIKYSKKGEKTNIKHERLIPQKQEGLYTVLFHPVGGQLSLDVLKELIEKIEN
                     IEDVEVRLGMDENLYIRNLNGKEAEAILDFTQGKGGDTLAERSVSCIGTPICQMGVLE
                     SQKTLREFVKLLKENSIEEGVLPRVRFSGCPNSCGIHEVGNIGFAGKKKKVGDVLSNV
                     FELHFGGKLGIGETRLGDYYGDILQDKVPEFLIKVAKAVEAKNSTFEEWIKDNSEEFK
                     AIVEEYNV"
     gene            1928..2743
                     /gene="speD"
                     /locus_tag="K8M77_000836"
     CDS             1928..2743
                     /gene="speD"
                     /locus_tag="K8M77_000836"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBI7085350.1"
                     /translation="MLGLKEKICLYGFNNLTKTLSFNIYDICYAKTEREKEDYIKYID
                     EQYNSERLTKILCDVTEMIGAHVLNISKQDYEPQGASVNVLITEEALPVALIDPSCNK
                     GELSYLELRDSVVGHLDKSHLTVHTYPEFHPNNDIISFRVDIDVSTCGKISPLNALDY
                     LIGSFDSDVITIDYRVRGFTRDVDGRKCYIDHDIKSIQDYIDGETLKKYDAMDVNVYQ
                     SNIFHTKMMLKDMVLNNYLFNSDPYELSPNDRREIRDRISKEMIEIYGGVNIY"
     gene            2761..4215
                     /locus_tag="K8M77_000837"
     CDS             2761..4215
                     /locus_tag="K8M77_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI7085351.1"
                     /translation="MKRLSQENAPIYEALKWYKNQRIVPFDVPGHKQGRGNTELREFL
                     GEDCVSVDVNSMKPLDNLCHPNSVIKDAEELAAEAFSADKAFFMVNGTTSAVQAMIMS
                     VCKDGDKIIMPRNVHRSAINSLIITGVTPVYINPGIHGRLGISLGMAMEDIKKAIKEN
                     PDAKAIFVNNPTYYGICSNLREMVKLAHENNMYVLVDEAHGTHFYFDKRLPVSAMEAG
                     ADMAAVSIHKTGGSLTQSSMLLMKCNRIKPDYVRTIINLTQTTSGSYLLLSSLDIARR
                     NLALNGEKIFEEVVGIAEYAREEINKIGGYYAFSKEINNGDDIYDFDVTKLTINTLET
                     GLAGIEVYDILRDDYGIQIELGDVANILAIISVGDTRYNIERLVSALSEIKRIHEKDK
                     KDIFDHEYIDPIVKVKPKEAFYAKKKKVLLRESEGLISGEFVMCYPPGIPILAPGELV
                     TKETIDYIEYSKKKGSLVTGPEDMEIEYINVLDF"
     gene            4230..5081
                     /gene="speE"
                     /locus_tag="K8M77_000838"
     CDS             4230..5081
                     /gene="speE"
                     /locus_tag="K8M77_000838"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBI7085352.1"
                     /translation="MELWYTEEHTDKVRFSIKVDEQLYSGKSDFQRIDVFKSQEFGTF
                     FTLDGLMMVTEKDEFIYHDMIVHVPMATNPNIKNVLVIGAGDGGTVRELTRYETIEKI
                     DMVEIDKLVVDVCREYLPQTANKLDDPRVSLFFEDGLKFVRSVENKYDLIIVDSTDPF
                     GPGEGLFTREFYGNCFKALKEDGILVNQHESPYYEEYARGMKRAHKRIKECFPVCRVY
                     QAHIPTYPSGHWLFGFASKKYDPLNANIEKWNDLGLKTKYYNTDLHKGCFALPNYVKE
                     MLENVDE"
     gene            5068..5925
                     /gene="speB"
                     /locus_tag="K8M77_000839"
     CDS             5068..5925
                     /gene="speB"
                     /locus_tag="K8M77_000839"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBI7085353.1"
                     /translation="MLMNRNVSTFIGCEAEYDESSIVVFGAPFDSTTSFRPGTRFASQ
                     VMRGESWGLESYSPYQDLDLYDFNIFDGGEIELPFGNSEGALALIEDFSTKVVEDGKI
                     PAMIGGEHLVTLGAFKGVFKKYPDVHVIHFDAHADLREDYLGQKLSHATVIHRVWDLV
                     GDNKIFQFGIRSGEKEEFLWAKDHVYTNKFNCDTLDYALEMVKDKPVYVTIDLDVLDP
                     SVFPGTGTPEPGGIQFNELLNSILKLRGLNIVGFDINELSPQYDQTGASTAVACKVLR
                     EMLLVANNK"
     gene            complement(6241..6504)
                     /locus_tag="K8M77_000840"
     CDS             complement(6241..6504)
                     /locus_tag="K8M77_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBI7085354.1"
                     /translation="MKELFVSYLVIINLVAIILMYIDKQKAKKHQWRISEDTLIGVSI
                     IGGGIGALWGMHVFRHKTKHAKFTIGIPLIIAIQALLILYFIR"
     gene            complement(6770..9439)
                     /locus_tag="K8M77_000841"
     CDS             complement(6770..9439)
                     /locus_tag="K8M77_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBI7085355.1"
                     /translation="MSAIIFMINKSIKIKGMSCAACAARIEKVLGKMDGISKANVNLA
                     TEKLNLEFDENKISFKEIEEKINKLGFSVVRNLKKESFKVSGMSCASCAARIEKVLNK
                     LSGIYNATVNFANESLQVEYDEDEISLEEIKVKVKKLGFELKGNNKSTSFKVEGMTCS
                     ACAARIEKVTSKMDGVESSNVNFANSTLNISFDKDKLSTNDIKAKVEKLGYKLLDASQ
                     EDEHEKAKENETKRMKNRLIGSAIFTIPLFIISMGHMVGLHLPNIIDPMHNPLNFALI
                     QLLLTTVVIFICRDFFIHGFKNLFMRSPNMDSLIAIGSGAAYVYGLFAIYHIYMGDHS
                     YAMQLYFESAGTILTLISLGKYLETLTKGKTSDAIKKLMGLAPKTATLLVDGKEKIVS
                     IDDVKVFDLILVKPGEKLPVDGKVVEGYTSIDESMLTGESIPSEKKVGDTVFGASINK
                     NGRIIYEATKVGKDTVISQIVKLVEDAQGSKAPIAKLADTISGYFVPIVISLAVVASL
                     AWYFSGESKTFALTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKSGEALES
                     TQNLNTVVFDKTGTITEGKPKVTDIICENISKDELLLLAASAEKGSEHPLGEAIVRDA
                     EEKNLKLKNVLDFEAIPGKGIKCSIEDKRILLGNYKLMKDKNINLKNLLATSEELASK
                     GKTPMFIAIDEKIAGIIAVADTVKETSKKAIKTLQKMGLEVVMLTGDNLKTAKAIAKE
                     VGVNRVIAEVLPQEKAEKIKTLQDEGKKVAMVGDGINDAPALAISDIGMAIGSGTDIA
                     MESADIVLMKGDILHVVGAIQLSRQTMKNIKENLFWAFGYNTLGIPVAMGVLHIFGGP
                     LLNPMIGAFAMNFSSVSVLLNALRLKKFKPNYK"
     gene            9731..10387
                     /locus_tag="K8M77_000842"
     CDS             9731..10387
                     /locus_tag="K8M77_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBI7085356.1"
                     /translation="MKKLLYITVNSKPEKLSSSKTIGRAFISRFVELNPEFEIEEVDL
                     YKDFIPRLEHKHFSGRSSVVDCETASIDEKTKEEVKRIIELSEQFKNADLYVIAAPLW
                     TLSFPAPLKEYIDCVVQNKITIKVTPEEVKGLLDDKQREMVYIQSSGGEIPWLTKGIF
                     NKGLNYVEDIMKFLGIKKFHPLLVDGTGFSIEEKFKAEDKAVEKIDNVIKDIHFSNKG
                     "
     gene            11668..12804
                     /locus_tag="K8M77_000843"
     CDS             11668..12804
                     /locus_tag="K8M77_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBI7085357.1"
                     /translation="MIEIKNVSKQIGDRKILDNISLTIDKGSLVVLIGSSGCGKTTTL
                     KLINKLITPTSGEIFINGKSLAKENPIELRRNIGYVIQNNGLFPHLTIKENIELIPRL
                     KKGKDVEKIEQTTLDLLNMVGLDPEIYLNKYPSELSGGQQQRVGFARAFATDAEIILM
                     DEPFSALDPITRTSLQEELFTIQEELKKTIIFVTHDMDEALKIADKICIMNGGKIAQY
                     DTPENILRNPANDFVRDFIGSDRVWNNPDFIKAKDIMIKNPVSVKGARTILQGIEIMR
                     SNKVDSLLVIDKENVLKGIVTFKDIKITNEKSRVLSEIMSENPLRVNEDDSLVDILTV
                     MNENSVGFIPVVNSEEKLVGLITRSSLLSILSDQFLDMEVNIFE"
     gene            12797..14350
                     /locus_tag="K8M77_000844"
     CDS             12797..14350
                     /locus_tag="K8M77_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI7085358.1"
                     /translation="MNSLLQYVISQKTQILDLLVQHIYLTITAIGIAILIGVPLGILV
                     SRVKFLRKPIIGFVNLVQAVPSMALLGLLIPILGIGSTPAIFMVVVYSLLPIVKNTYT
                     GISGIDPVVLESAKGIGLTKNQSLFKIQLPLALPIIMSGIRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPACILALIVDFIFGKIEVAVTPKGLSNDKKKKN
                     TFVLKIISVIMIIAILFMGISSFISSKKDKVVIGSKNFTEQLILGNMYADLVQAKTDL
                     QVEKKLNLGGTSVAFGALEKGDVDMYVDYTGTLLVNVMKENNIDNSVDYYNSIKENMN
                     KEHGLTVMEPLGFNNTYNIAISKELADKYKINTISDLSKYSNDFVLSPTIEFQNRQDG
                     LVGLKNYYGMDFKNVKSLDGSLRYSALSNGESQAIDAFSTDGLLKKFDLKTLEDDKKF
                     FVNYSAVPIVNNKTLEKYPQLKDVLNSLSGKINEEKMIDLNYEVDVLGKSPEEVAKAF
                     LIREGLIEQ"
     gene            complement(14682..16868)
                     /locus_tag="K8M77_000845"
     CDS             complement(14682..16868)
                     /locus_tag="K8M77_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBI7085359.1"
                     /translation="MKRSYRDMAIIRKRLSFILAFIIIMCFILSIRLSYVMIVKGPEY
                     AELAVEQWTNEVKISARRGKILDRDLNELAVSSNVYRVDFNLNAIRSYTKKTGKTNEE
                     LALEISKVLEMDKDTVLKKLEFKLPSGAPAGAAILARRIEKEKADKVKELEIPGVMVS
                     PDTQRYYPNDNFLSHVLGTTNSDGVGLNGIELKYDRVLSGSPGLKITEMVKDGAEYPY
                     TISRFTAPVDGKDVVLTIDEKLQYFAEQIAEIGLVHHNADAASVIIMNPNNGEILAMS
                     NKPDFNPNKPYDGAENFSGNTSSDRIQKMWRNRSVSDSFEPGSIFKIITASAAVQEGV
                     AGKDETYTCSGGLQKGDRFIKCWKRGGHGTQTFEEIIQNSCNVGFMNLGEKLGAEKLN
                     EYIKKFGLGKKSGVDLPGESPGIVKKTENITDMDLATISFGQTNTVNPIQFMTAVNAV
                     ANGGTLIQPHVVKEISYINENNDRVIDETFVPKKTENLISKETADQIKLALENAVKNG
                     SAKGAYKEGYGVAGKTGTAQKVNPETGGYGGGYVASFVGFAPYDNPQISVMVSVDNPK
                     NGEYYGGRVAAPLAGMLFQNIFNYIDLTEFHLDEKLPEKETVVIPEVRGLPINDATSM
                     LKELGISYEIEGEGEFIVESNPAPGYPISPDTKITLIAGDSSNLNSDVIIPDFKNYSK
                     ESAEKLLNQLGLIGEFVGEGPSVTKQSISPDEVVNGNSKITLTLGY"
     gene            17210..17878
                     /locus_tag="K8M77_000846"
     CDS             17210..17878
                     /locus_tag="K8M77_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7085360.1"
                     /translation="MEHNKIKNINLSKRRKKIFRRRLIFLISIILIICGSIFFVFSKM
                     NKDESYRDAYKKNISSRELEKMRQELDIKEVNYKWGSGLKKGNSPKRLIIHHSATDSP
                     ETPEDIHKFHLDNGWSGIGYHFYIREDGTIYKGRDENVIGAHAKNANYNTLGICIEGN
                     FEKEGLKEAQKNSLVKLGTYLSLKYPIKDILPHREVVDTLCPGTLFPMDNIKSSIIEE
                     IKNI"
     regulatory      17968..18085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            18358..19455
                     /gene="ribD"
                     /locus_tag="K8M77_000847"
     CDS             18358..19455
                     /gene="ribD"
                     /locus_tag="K8M77_000847"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBI7085361.1"
                     /translation="MDEFFMKKALELARKGEGYVNPNPLVGAVIVKGGEIIGEGYHEF
                     FGGNHAEINAINSSIKSTEGATIYVTLEPCCHYGKTPPCVEAIIKNKFKRVVVGTLDL
                     NPLVSGKGIRILREAGIEVKVGVLEKECIRLNEIFNFYIKENRPFIALKWAMTLDGKI
                     ATKDFKSKWITNKKSREFVHSLRNKYSSIMVGINTVLKDNPDLRCRLNKNKELERKHF
                     RIVLDSKLKIPLDSEILKNQSESKTLIFTTNKKDKMKNEKLKDKGIEVIEVNLDEGKI
                     NINQVLECLKEKNIDSVLIEGGGNLNFTFLENKKVNKIYAFVAPKVFGGKNAITPVEG
                     EGIRNVLDAFEFPEVNLKTFHNDILIECSLS"
     gene            19477..20130
                     /locus_tag="K8M77_000848"
     CDS             19477..20130
                     /locus_tag="K8M77_000848"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBI7085362.1"
                     /translation="MFTGIVEEIGEVISIDKNEKSSLLKIRANKVLEDTKIGDSISTN
                     GVCLTITTKGKDYFTAYVMGETLRKSNLGNLKKSSKVNLERAMSINGRFNGHIVTGHI
                     DSTGEIISFKDEGEAIWVEIKVSYELSKYIVYKGSIAIDGISLTIAEVKGESFKVSVI
                     PHSQEETTLTKRKIGDLVNIECDPIGKYVEKLLGFKKDSRKKKSVLTEEFLTINGFI"
     gene            20279..21478
                     /locus_tag="K8M77_000849"
     CDS             20279..21478
                     /locus_tag="K8M77_000849"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBI7085363.1"
                     /translation="MKKFNLVEEAIEDIKNGKMVIVIDDEGRENEGDLVMAGELVTGE
                     KINFMATYGKGLICTPVSESIAENLGLNPMVSHNTDNHETAFTVSIDYKDTTTGISAF
                     ERAYTIKELINEESKAEDFRRPGHVFPLIAKNGGVLKRAGHTEAAIDLAKLAGLKEVG
                     VICEIMKDDGTMARTDELLEFAKIHNMKIITIEDLIAYRTERDNFVYRVVETNMPTKY
                     GEFKAIGFIDKLTGEHHVALVKGEIKPEEPVLLRVHSECLTGDVFGSRRCDCGEQFHR
                     AMEKINEEGKGILLYMRQEGRGIGLINKLKAYKLQDEGLDTVEANIALGFPEDLRDYG
                     VGAQILKELGVKEIRLMSNNPKKITGLEGYGLKIVERVPIEIKCNKENEFYLKTKKEK
                     MHHLIMQ"
     gene            21605..22066
                     /locus_tag="K8M77_000850"
     CDS             21605..22066
                     /locus_tag="K8M77_000850"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBI7085364.1"
                     /translation="MRILEGNLIGQDKKFAIVAGRFNEFIVSKLIGGALDAFKRHGVE
                     EENIDLAWVPGAFEIPLIAKKLAKSGKYAGVVCLGTVIRGATSHYDYVCGEVSKGIAN
                     VSLDTEVPVIFGIVTTENIEQAIERAGTKAGNKGFDAAIAAIEMANLLENI"
     gene            complement(22193..23065)
                     /locus_tag="K8M77_000851"
     CDS             complement(22193..23065)
                     /locus_tag="K8M77_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HBI7085365.1"
                     /translation="MKINHKITSIVLTLGLISSSIYTINVNAETLNSKIQVSNLNLSN
                     SYNIKNENLIKSSKEGWINTNGIWYFYKNGIMQTGWLNDNGIWYYLNENGSMATGWYK
                     VDSNWYYSNSSGVMQTGWLNDNGNWYYLNDNGSMATGWYKVDSNWYYSNSNGVMQTGW
                     LNDNGTWYYLNDNGSMATGWYKVYSKWYYSNSNGVMQTGWLNDNGTWYYLNSSGEMVT
                     NTIIDGWKIDSNGIATPLKDENHSIVYVTPNGKSYHYSKDCTALKRSSSILSMSLNEA
                     INKGKSDPCNLCVK"
BASE COUNT         8665 a         2742 c         3891 g         7789 t
ORIGIN      
        1 ataaagaaag aattgtttac taatggaatt tagaagcata aaattttagt gaggtgatat
       61 aaaatgaata cattaaatga tattttatca aaggaaattg aagaatttag agaaaaagga
      121 cataagtttt taaataaaga aatgactgtt ggtgaattta gaaaggtatc aggaggaatg
      181 ggagtttacg cacaccgtgg aggaaaagaa ttcgtggtga gattaagaat tccatctgga
      241 gttgctagat taagttcatt agaacaagta tataattggg ctaagaaata taacctagaa
      301 aaaatacatt taacaactcg tgaatcaata cagttacatg gaatatcaat agatgctgta
      361 tgcgacataa tgcaagatgg gttaactaaa ggaatataca ctagaggcgc tggtggaaac
      421 tttccaagaa acgtagcatt aaatccttta tcaggagttc aaataggaga aacttttgat
      481 gtaactcctt atgcactagc agctaatgcg cattatatga gtaaaatata tacttataat
      541 ttaccaagaa aaattaaaac tgcattttca aatacagaag aagattcagc tcattgcaca
      601 gttacagact taggattttt agctgtggag aataaaggta agagagcatt taaactttac
      661 ataggtggag gattaggaag aaatccaaga aaatctgttg aatttgatga gttaatagat
      721 gaaaaggacg tattatattg tcttgaagct ataactaatt tatttgtagc tgaaggagat
      781 tataaaaata attcaaaagc tcgtataaga tatattgttg agagaatggg agaagaagag
      841 tttaaaaatt gttttagaaa gcatttagaa gaagttaaag ctaagggggg attagacctt
      901 aaggttgaag atataaaata tagtaaaaaa ggtgaaaaaa ctaatataaa acatgaaaga
      961 ttaatacctc aaaaacaaga aggattatat actgttttat tccacccagt tggaggacaa
     1021 ttaagcttag atgtattaaa agaattaata gaaaaaatag aaaatataga agatgtagaa
     1081 gtaagattag gtatggatga aaatctatat ataagaaatt taaatggaaa agaagcagaa
     1141 gctatcttag attttactca aggtaaagga ggggatactt tagctgagag aagtgtttct
     1201 tgtataggta caccaatatg ccaaatggga gtattagaaa gccaaaaaac tttaagagaa
     1261 tttgtaaaat tattaaaaga aaattcaatt gaagaaggag tattaccaag agttcgtttc
     1321 tcagggtgtc cgaattcttg tggtattcac gaagtaggaa atatagggtt tgctggtaaa
     1381 aagaaaaaag ttggtgatgt tttatcaaat gtatttgaat tacactttgg tggaaaatta
     1441 ggcataggtg aaactcgttt aggagattac tatggagata tacttcaaga caaggttcca
     1501 gaattcttaa ttaaggttgc aaaggctgta gaagctaaaa attcaacttt tgaagagtgg
     1561 attaaggata atagtgaaga atttaaagct atagttgaag aatataatgt atagctgtta
     1621 acaaaatatt tacaaaatcc aaaaaaactt aataaataag tttttttgga ttttttttat
     1681 ttttacttaa aattcctttg aataggcata tttaaatact tttttagttt attttattaa
     1741 atattaggtt tagtaatact aagtatttta cttagttttt gttaccgaaa tattaacgat
     1801 gacctttgaa gatagcttga aataagggtt agaaagaatt tgctatagaa aaaaataaaa
     1861 tggtgatata taataaattc aaaatttata tttatgcaat taaataagtt gtttaaagaa
     1921 aggattgatg ttagggttga aagaaaagat atgcctttat ggttttaata atcttactaa
     1981 gacactaagt tttaatattt atgatatttg ttatgcgaaa acagagagag aaaaggaaga
     2041 ctatataaaa tatatagacg agcagtataa ttctgaacgt cttacaaaga ttctttgtga
     2101 tgttacagaa atgataggag ctcatgtcct aaatatttca aaacaagatt atgaacctca
     2161 gggagctagt gtaaatgttc ttataacaga ggaggcatta ccagtagcac taattgaccc
     2221 atcatgtaat aaaggagaac tttcatattt ggaacttaga gatagtgtag ttggacattt
     2281 agataaaagt catcttacag ttcatactta tccagagttt catccaaata acgatataat
     2341 ttcttttaga gttgatatag atgtatctac ttgtggaaaa atatctccat taaatgctct
     2401 agattactta atcggaagtt ttgattcaga tgtaataact attgattata gagttagagg
     2461 atttactaga gatgttgatg gtagaaaatg ctacatagac catgatataa aatctattca
     2521 agattatata gatggagaga ccctaaaaaa atacgatgct atggatgtta atgtttatca
     2581 atctaacata ttccatacaa agatgatgct taaggatatg gttttaaata actatttatt
     2641 taattctgat ccatatgaat tatctccaaa tgatagaaga gaaataagag atagaatatc
     2701 aaaagagatg attgaaatat acggtggtgt aaatatttat taaggaaagg agggatttct
     2761 gtgaaaagac tttctcaaga aaatgctcct atttatgaag cattaaaatg gtacaagaat
     2821 caaagaattg ttccttttga tgtaccaggt cataaacaag gaagaggaaa tacagaatta
     2881 agagagtttt taggtgaaga ttgtgtttca gtagatgtaa attcaatgaa acccttagat
     2941 aatctttgtc atccaaattc tgtaataaag gatgcagaag aattagcagc tgaggccttt
     3001 agtgctgata aggcattttt catggttaat ggaaccacat cagctgtaca agccatgata
     3061 atgagtgttt gcaaagatgg agataagatt ataatgccta ggaatgtgca tagatctgca
     3121 ataaattctt taataataac aggggtaact cctgtgtata taaatccagg aattcatggt
     3181 agacttggaa tttctctagg aatggcaatg gaagatataa aaaaagccat taaggaaaat
     3241 ccagatgcta aggctatttt tgttaataac ccaacatact atggaatttg ctctaattta
     3301 agagaaatgg ttaagttagc tcatgaaaat aatatgtatg tcttagttga tgaagctcat
     3361 ggaactcatt tttattttga taaaagactt ccagtatcag ctatggaggc aggggcagat
     3421 atggcagcag ttagcataca taaaacagga ggctcattaa ctcagagttc tatgttactt
     3481 atgaagtgta atagaattaa gccagattat gtgagaacaa taataaactt aacacaaaca
     3541 actagtggtt cttatttact tttatcttct ttagatattg ctagaagaaa tttagcatta
     3601 aatggagaaa aaatatttga agaggttgtt ggaatagctg aatatgcaag agaagaaata
     3661 aataagatag gtggttatta cgctttctca aaagaaataa ataatggtga tgatatttat
     3721 gattttgatg taactaagct tactataaac actttagaaa ctggacttgc tggaatagaa
     3781 gtttatgaca tattaagaga tgattatgga attcaaatag agcttggaga tgtggctaat
     3841 attcttgcca taatatcagt tggagatact agatataaca ttgagagatt agtttcagct
     3901 ttaagtgaga ttaagagaat tcatgaaaaa gataaaaaag atatttttga ccacgaatat
     3961 atagatccta tagttaaagt taaaccaaaa gaggcttttt atgctaaaaa gaagaaggtt
     4021 cttttaagag aaagtgaagg attaatttct ggagaatttg taatgtgtta tccaccagga
     4081 attccaatac ttgccccagg agagcttgta actaaggaga ctatagatta tattgagtat
     4141 tctaagaaaa agggaagttt agttacaggc cctgaagata tggaaattga atatataaat
     4201 gttttggatt tttaggagga atttaaatta tggaattatg gtacacagaa gaacatacag
     4261 acaaagtacg tttttcaatc aaagtagatg aacaacttta tagtggaaag agcgatttcc
     4321 aaagaataga tgtttttaaa tctcaagagt ttggaacttt cttcacatta gatggactta
     4381 tgatggtaac agaaaaggat gaatttattt atcatgacat gatagttcat gttccaatgg
     4441 ctacaaatcc taatataaaa aatgtactag ttataggagc aggagatggt ggaactgtaa
     4501 gagaacttac aagatatgaa actatagaaa aaatagacat ggttgaaatt gataagttag
     4561 ttgttgatgt ttgtagagaa tatttacctc aaacagctaa taaattagat gatcctagag
     4621 taagtttatt ctttgaagat ggattaaaat ttgtacgttc agttgaaaat aaatatgact
     4681 taataatagt tgattcaaca gatccctttg gacctggaga aggtttattc acaagagaat
     4741 tttatggaaa ttgctttaag gccttaaaag aagatggaat acttgtaaat caacatgaaa
     4801 gcccatacta tgaagagtat gcaaggggga tgaagagggc tcataagaga ataaaagaat
     4861 gtttcccagt atgtagagtt tatcaagctc atataccaac ttatccatca ggacattggc
     4921 tatttggttt tgcatcaaaa aaatatgatc cactaaatgc taatatagaa aagtggaatg
     4981 atttaggatt aaaaactaaa tattataaca ctgatttgca taaaggatgt tttgcattac
     5041 ctaattatgt taaggagatg ctagagaatg ttgatgaata gaaatgtaag cacttttata
     5101 gggtgtgaag ctgaatatga tgaatcaagc atagttgtat ttggagctcc ttttgattca
     5161 acaacttctt ttagaccagg tactaggttt gcaagccaag tgatgagagg agaatcttgg
     5221 ggattagaaa gttatagtcc atatcaagac ttagatcttt atgattttaa tatttttgat
     5281 ggaggagaaa tagagcttcc tttcggaaat tcagagggag cattagcttt aatagaggat
     5341 ttttcaacta aggttgttga agatgggaaa ataccagcta tgataggtgg agagcattta
     5401 gtaactttag gggcatttaa aggagtattt aaaaaatatc ctgatgttca tgtaattcac
     5461 tttgatgctc atgcagattt aagagaagat tatttaggtc aaaaactatc tcatgctact
     5521 gttatacata gagtttggga tttagtagga gataataaaa tattccaatt tggaatacgt
     5581 tctggtgaaa aagaagaatt tttatgggca aaggatcatg tttatacaaa taagtttaat
     5641 tgtgatactt tagattatgc cttagaaatg gttaaagata aacctgttta tgtaacaata
     5701 gacttagatg ttttagaccc atctgtattc ccaggtacag gaacaccaga accaggtgga
     5761 attcagttta atgagcttct aaattcaata ttaaaactta gagggttaaa tattgtggga
     5821 tttgatatta atgaattatc accacagtat gaccaaacag gagcatcaac ggctgtagct
     5881 tgtaaagttt taagagagat gttattagta gcaaataaca aatagaatga ttttaaagat
     5941 agattagcct tagataaatt taggatatgt atttaaaaat tagttaaaat taaaactagt
     6001 tcttaatcaa ttcagttaag ggtatattta gaaattttta aagttaatta caatttatgc
     6061 aaaatatact ttaagttaaa tttaatttaa aattcttaga tatttagata taaaaaatag
     6121 aactgtatca aaagctaata aaaaatttct taaattaatg agagagagta tttattaact
     6181 ctttcaaaaa tagtcttaga aattttttga aggttaattt gatacagttc ttttgagtat
     6241 ttatctaata aagtaaagaa ttaaaagtgc ttgaattgct attattaaag gaatacctat
     6301 agtaaacttt gcatgttttg ttttatgacg aaaaacatgc attccccata atgctccaat
     6361 tccgcctcct attatggaaa cacctattaa ggtgtcctca ctaattctcc attggtgttt
     6421 tttagctttt tgtttatcta tatacattag aattattgcc actaaattta ttataactaa
     6481 ataacttaca aaaagttctt tcaataatta cccctcctaa tttttctcat tagtatttat
     6541 tatatggttc ttaacttaat tataacttat ttataggaga atttattatt aatataaaaa
     6601 agatatagga tttaatatat aaaatatcta tcctatatta tcatacctta tttaaataaa
     6661 agcactaaat tttattttat taaaggctat ataaaaattg tggaaatgta ggaactttta
     6721 ttaaaaataa attttcctac atttttttga ataatgtata tttaaaagct tatttatagt
     6781 ttggtttgaa tttttttagt cttaaggcat ttaataatac tgatactgaa ctaaagttca
     6841 tggcaaaggc acctatcatt ggattaagta atgggcctcc aaatatatga agaactccca
     6901 ttgctactgg aatacctaaa gtgttgtatc caaaggccca gaataagttt tcctttatat
     6961 ttttcattgt ttgcctactt aattgaatag caccaacaac atgtaatata tcacctttca
     7021 ttagaactat atcagcagac tccatagcta tatcagtacc tgaacctata gccattccaa
     7081 tgtcagaaat agctaaagca ggagcatcat ttattccatc accaaccata gcgacttttt
     7141 tgccttcatc ttgaagagtt ttaatttttt ctgccttttc ttgaggaaga acctcagcaa
     7201 taactctatt tacacctact tcttttgcaa tagccttagc tgtttttaaa ttatctcctg
     7261 ttaacattac tacttcaagt cccatttttt gtagagtttt tatagctttt ttacttgttt
     7321 ctttaacagt atcagcaaca gcgattatac cagctatttt ttcatcaata gcaataaaca
     7381 taggagtttt tccctttgaa gctaattctt cagaagtcgc caataaattt ttaagattta
     7441 tatttttatc tttcataagt ttatagttac ctaataaaat tcttttatct tcaatagaac
     7501 acttaattcc cttaccaggt attgcttcaa aatctaaaac attttttagt ttgagatttt
     7561 tttcttcagc atctctaaca atagcttctc caagaggatg ttctgaacct ttttctgctg
     7621 aggctgctaa taaaaggagt tcatcttttg aaatattttc acaaataata tctgtaacct
     7681 taggttttcc ttcagttata gttcctgttt tgtcgaagac aacagtattt aaattttgtg
     7741 tactttctaa agcttcacca cttttaataa gtattccatt ttcagctcct ttacctgtac
     7801 caaccataat agctgttggg gtagctagac ctaaagcgca tggacaagcg attactaata
     7861 cagatataaa tattgtaagt gcaaaagttt tactttcacc agaaaaatac caagctaaac
     7921 ttgcaactac tgctaaagat attactatag gtacgaaata acctgaaata gtatcagcta
     7981 attttgcaat tggagctttt gatccttgag catcttctac taacttaact atttgagaga
     8041 ttacagtatc tttacctacc tttgtagctt cataaattat tcttccattt ttatttatac
     8101 tagctccaaa gactgtatct cctacttttt tttcagaagg aatactttct ccagttagca
     8161 tactttcatc aatggatgta tatccttcta caactttacc gtctacaggt aatttttcac
     8221 ctggcttaac aagtattaaa tcaaaaactt taacatcatc aatagaaaca attttttctt
     8281 tgccatctac aagtagagta gctgttttag gggctaatcc cataagtttt tttattgcat
     8341 cagaagtttt acctttagtt aaagtctcta agtatttacc taaacttatt aatgtaagta
     8401 ttgttccagc agattcaaaa tataactgca tagcatagct atggtcaccc atataaatgt
     8461 ggtatattgc aaataatcca taaacatatg cagcaccaga tcctatagct attaaggaat
     8521 ccatattagg acttctcata aataaattct taaaaccatg tataaagaaa tctctacata
     8581 tgaatataac tacagttgta agaagcaatt gaataagtgc aaagtttaag ggattatgca
     8641 ttggatctat tatattaggt aaatgaagac caaccatatg tcccattgat attataaata
     8701 atggaatagt gaatattgct gagccaataa gtctattttt cattctttta gtttcattct
     8761 cttttgcttt ttcatgttca tcttcttgag atgcatctaa aagtttatat cctaattttt
     8821 ctacctttgc ttttatatca ttagtagaaa gtttatcttt atcgaaagaa atattaagag
     8881 tagaatttgc gaagttaaca ttagaacttt caacaccatc cattttactt gttacttttt
     8941 caattctagc tgcacaggct gaacaagtca ttccttctac tttaaaggaa gtagacttat
     9001 tatttccttt tagttcaaag cctagttttt ttacttttac tttaatttct tctaaggaaa
     9061 tttcatcttc atcatattca acttgaaggc tttcattagc aaagttaaca gtagcattat
     9121 atattccaga taatttattt aaaactttct caattctagc tgcgcaggaa gcacaactca
     9181 ttccagaaac tttgaaagat tctttcttta agtttctaac tactgagaaa cctagcttat
     9241 ttattttttc ttctatttct ttaaaggata ttttattttc atcaaactct aaatttaatt
     9301 tttcagttgc caaattaaca ttagcttttg atatgccatc cattttacct agaacttttt
     9361 cgattctagc agcacaagcg gcacaactca taccttttat tttaattgat ttatttatca
     9421 taaaaattat agctgacata ttcccttgtt ataaagaaag gaataacagt tttcctcctt
     9481 ttaaattttt acctccccta gggggtggtt gtgattcaaa tatactcttt gatctttctt
     9541 ttgtcaataa taagctgata aggattatca atgaaattta agatttaggg caagtatttt
     9601 taataatata agtatatttt gattaaaaaa taaaaactta tttaaaatta gataattatt
     9661 aataaaaaac taaaaaaatc acaagataag aaaaagaagt taatttagaa aatttaaatg
     9721 aggtgagtat atgaaaaagc tactttatat tacagtaaac tcaaagccag agaagctttc
     9781 atcaagtaag actattggaa gagcttttat aagtagattt gtagaattaa atccagagtt
     9841 tgaaatagag gaagtggact tatacaaaga ttttattcct agattagagc ataagcactt
     9901 ttcaggaaga agttctgtgg tggattgtga gactgctagt atagatgaaa agacaaagga
     9961 agaagtaaaa agaataatag aattaagtga gcaatttaaa aatgcagatt tatacgtaat
    10021 agcagcccct ttatggacac tatcttttcc agcaccatta aaagaatata tagattgtgt
    10081 tgtccaaaac aaaataacaa ttaaagtaac accagaagag gttaagggat tgttagatga
    10141 caagcaaaga gaaatggttt atatacaatc ttcaggggga gaaattcctt ggcttactaa
    10201 agggatattt aataaaggct taaattatgt tgaagatata atgaaattct taggaataaa
    10261 aaaatttcat ccattattag tagatggaac aggttttagc atagaagaaa agtttaaggc
    10321 tgaagataag gctgttgaaa aaatagataa tgtaataaaa gacatacatt ttagtaataa
    10381 aggttaaaat aaaagctttt tatttagtta agaaaatttc atattaaaat taaatttggt
    10441 tatatttaaa tattgatttt taggttgatc aatagagtta agagaattta tcaaagtaat
    10501 ttattaataa aacttactag taaataacat attaattgta agttttattc atgagatttg
    10561 ataaattctt ttactttaaa tataagaaaa aatatctttt acacaagtat tataaattaa
    10621 aaagagaacg aatgtttgca tttcttactt ttatatgtta tgcttaactc tcagcttgtg
    10681 ggtttaaatg ctgaaattag aatatagaaa taaattattt ttataataaa ataaagttta
    10741 gagtttattc tcaggtttaa aaaggtatag aagagagata aaatatttaa attacaataa
    10801 cggtaaattg taattcactt cgtaaagagg attttcactt caataggtac aaaattattt
    10861 tatactaatg ttttaaaatt tctaaaaaca tgataaatgg tataaaaaca taacacctaa
    10921 aatattctta ataatccagt aaaaaattta aaagtatgtt attaattcag tatagtttta
    10981 cattatagga ctatttattt aagatttata aaaaggtact gcgtgaaaaa ttaataatat
    11041 gtattttcat atattatagc taattaaagt tgatttagag tgatatttga ttagtttaat
    11101 ttttgttgaa aaaatagaat atattcatac tagttgttaa ctcaaaagag aaactagatg
    11161 atttttaata cttacaatta aacctgtgaa ttaaaggaaa aaaataaaaa ttttgatata
    11221 taaatattaa attatgtcta taatttaagt gaaagttgaa ttctctatat agggagagtt
    11281 tatttaaaat gtaaattaaa aggaggatga aaatttgtta atatataata tttgtaatga
    11341 atttagtaaa agtaaaaaga ataaaagaag aactagtaca gatgtaaaga atattttaaa
    11401 tgaggcaaaa aataaataga ttttattatg tagagcactg ttttatataa tggtgttttt
    11461 ttattatttt aaatactatt ttaaaagctt atgaagatag taatatagcc attaatagag
    11521 actaatatag tatttagtat ttaataaatt atatgaaata aattttaggt taaacaaatt
    11581 aaaatttgaa tttaacttaa aatcgtgtta caataaacta acaactcttt aaaaaataaa
    11641 agttgtcaga agaaaagggg gatttttatg atagaaataa aaaatgtatc taaacaaata
    11701 ggggacagaa agatactaga taatatttct ttaactattg ataaaggaag tctagttgtt
    11761 ttaataggtt ctagtggatg cggtaaaaca actactttaa aacttataaa taagttaata
    11821 acacctactt ctggtgaaat atttataaat gggaaatctt tagccaagga aaaccctatt
    11881 gaattaagaa gaaatattgg atacgtaatt cagaataatg gattgtttcc acatttaaca
    11941 ataaaggaaa atatagagct tattccaaga ctaaaaaagg gcaaagatgt tgaaaaaata
    12001 gagcagacaa ctttagacct tttaaatatg gttggattag atcctgaaat ttatttaaat
    12061 aagtatccat cagagcttag tggaggacaa caacagagag taggttttgc aagagccttt
    12121 gctacagatg ctgagataat tttaatggat gagcccttta gtgctttaga tccaataact
    12181 cgtacttcac ttcaggaaga gctttttact attcaagagg aacttaaaaa gacaattatt
    12241 tttgttactc atgatatgga cgaagcttta aagatagcag ataaaatatg cataatgaat
    12301 ggaggaaaaa tagctcaata tgatactcca gaaaatatac ttagaaatcc agcaaatgat
    12361 tttgttagag attttatagg atcagatagg gtttggaata atcctgactt cataaaagca
    12421 aaggatataa tgataaaaaa tcctgtatct gttaaaggtg ctagaaccat attacaggga
    12481 attgaaataa tgagaagtaa taaggttgat agtttattag ttattgataa agaaaatgta
    12541 cttaaaggta ttgttacttt taaagatata aaaattacaa atgaaaaatc aagagtactt
    12601 tcagaaatta tgagtgaaaa tccactaaga gttaatgaag atgatagttt ggttgatatt
    12661 ttaactgtaa tgaatgaaaa ctcagtagga tttatacctg ttgttaactc ggaagaaaaa
    12721 ttggtagggc ttataacaag aagtagttta ctttcaatat taagcgacca attcttagac
    12781 atggaggtga atatatttga atagtttatt acaatatgta atttcacaga aaactcaaat
    12841 attagattta ttagttcaac atatatattt aacaattacg gctataggaa tagctatatt
    12901 aataggagtt cctttaggaa ttcttgtttc aagggttaag tttttaagaa agcctataat
    12961 aggatttgta aacttagtac aagctgtgcc ttctatggcc ttactaggat tgcttattcc
    13021 catattagga ataggaagta cacctgcaat ctttatggtt gtagtatatt cattgcttcc
    13081 aatagttaaa aatacttaca caggaatctc tggcatagac ccagtagtat tagaatctgc
    13141 taagggaatt ggacttacta agaatcaaag tttatttaag atacaattac cattagcatt
    13201 gccaataata atgtctggta taagaatatc agcagttaca gccgtagggc ttatgacttt
    13261 agcagccttt attggtgccg gtggacttgg atacttagta ttctcaggag ttcaaacggt
    13321 aaataataat atgattttag caggagctat accagcttgt attttagcct taatagttga
    13381 ctttattttt ggaaaaatag aggtagctgt tacaccaaag ggattaagta atgataagaa
    13441 aaagaaaaat acttttgttc ttaaaataat aagtgtaata atgataatag ctattttatt
    13501 tatgggaatt tcatcattta tatcaagtaa aaaagataaa gtggttattg gatcaaaaaa
    13561 ctttacagag caacttattt taggaaatat gtatgcagat ttagttcagg ctaaaacaga
    13621 tttacaagtt gaaaaaaagt taaatctagg aggaacatca gttgcctttg gagcacttga
    13681 aaaaggtgat gttgacatgt atgttgatta tactggaaca ttacttgtta atgttatgaa
    13741 agaaaataat attgataata gtgtagatta ttataatagc ataaaagaaa atatgaacaa
    13801 ggaacatggg ttaacagtaa tggaacccct aggttttaat aatacttata atatagctat
    13861 atcaaaagaa ttagctgata agtataaaat aaataccata tcagatttat caaagtacag
    13921 taatgacttt gtattatctc caactattga gttccaaaat agacaagatg gtttagttgg
    13981 attaaagaat tactatggca tggatttcaa aaatgttaaa tctttagatg gaagtcttag
    14041 atactcagca ttatcaaatg gggaatcaca ggctatagat gctttctcaa cagatggact
    14101 tcttaaaaag tttgatttaa aaactttaga agatgataag aaattctttg taaattatag
    14161 tgcagtacct atagttaaca ataagacttt agaaaaatat ccacaattaa aggatgtttt
    14221 aaactcttta agtggtaaga tcaatgaaga aaaaatgatt gacttaaact atgaagtaga
    14281 tgtattaggt aaatcaccag aagaggtggc taaagctttc ttaattagag aaggtttaat
    14341 agaacaatag tgagtattta ttataatata gaaaaattta tttcaaagaa aatgagagta
    14401 cttatgtact ctcattaatt aaataatgtt taacacctaa aatagcaaag gaaaaagttt
    14461 cgatgtagta aaggctaatt taggaaagcc acttaagtat caaacagttt taaaaaaagt
    14521 taataatgtt tacaaattat ttataaaact ttttgtattt aatgtcaaac aaatggaatt
    14581 aaataatttt aatatttaag cctatatttt gaaattaaat aattttaatc attaaataat
    14641 tatttaaata aaggatgtat acattttata ttaacaattt tttaatatcc taaggttaat
    14701 gtaatttttg aatttccatt tacaacttca tctggtgaaa tactttgttt agttacagat
    14761 ggaccttcac caacaaattc tcctataagg cctaactgat taagaagttt ttcagcagat
    14821 tctttagaat agtttttaaa gtctggaatt ataacatcac tatttaagtt tgaactatca
    14881 ccagctatta aagttatttt tgtatctgga gatattggat agccaggagc aggattactt
    14941 tcaactataa attctccttc accttctatt tcgtaagata tacctaattc ttttagcatg
    15001 cttgtggcat catttatagg taatccacga acttcaggaa ttacaacagt ttctttttca
    15061 ggaagttttt catctaagtg gaattctgtt aaatctatat agttaaatat attttggaat
    15121 agcattccag ctaaaggtgc tgcaactcta ccaccataat attctccatt tttaggatta
    15181 tcaacagata ccataacaga aatttgtgga ttatcataag gagcaaaacc aacaaaggaa
    15241 gctacatatc ccccaccata tccaccagtt tcaggattaa ctttttgggc agtacctgtt
    15301 ttaccagcaa ctccatatcc ttctttataa gctccttttg ctgatccatt ttttactgcg
    15361 ttttctagtg ccaattttat ttgatcagca gtttctttgc ttattaaatt ttctgttttc
    15421 ttagggacaa aggtttcatc tataactcta tcattatttt catttatata acttatttct
    15481 ttaaccacat gaggctgaat taatgtgcca ccatttgcaa cggcattaac tgcagtcatg
    15541 aattgaattg ggttaacagt attagtttga ccaaaagaaa tagttgctaa gtccatatct
    15601 gttatatttt ctgttttctt aactattcca ggagattcac caggtaaatc tacaccagat
    15661 tttttaccaa gaccaaattt ttttatatat tcatttaatt tttcagcacc taatttttct
    15721 cctaaattca taaatccaac gttacaagaa ttttgtatta tttcttcaaa ggtttgagtg
    15781 ccatgaccac ctcttttcca gcatttaatg aatctatcac ctttttgaag accaccacta
    15841 caggtatagg tttcatcctt accagctaca ccttcttgga ctgcggcgga agctgttatt
    15901 atcttaaaaa tagaaccagg ctcgaaggag tctgaaacag atctatttct ccacattttt
    15961 tgaattctat ctgaggaagt atttccagaa aaattttcag ccccatcata aggtttattt
    16021 ggattaaagt ctggtttgtt agacatagct aagatttcac cattgtttgg attcattatt
    16081 attactgatg ctgcatcagc attatgatgt actaaaccta tttcagcaat ttgttcagca
    16141 aagtattgta atttttcatc aattgttaag actacatctt tgccatctac aggagctgta
    16201 aatctagaaa tagtataagg atattcagca ccatctttaa ccatctcagt tatttttaat
    16261 ccaggacttc cagataaaac tctatcatat tttagctcaa taccatttag tccaacacca
    16321 tcagagttag tagtacctag tacatgagaa agaaaattat cattaggata atacctttga
    16381 gtatcaggag aaaccattac tcctggtatt tctaactctt taactttatc agctttctct
    16441 ttttctattc ttctagcaag gattgcagca ccagcaggag ctccggaagg aagtttaaat
    16501 tctaattttt ttaaaactgt atccttatcc atttcaagaa ccttagaaat ttctaaagct
    16561 aattcctcat tggttttccc agttttcttt gtataagatc taattgcatt taggttgaag
    16621 tcaactctat aaacgttaga actaacagca agctcattta gatctctatc tagtattttt
    16681 cctcttcttg cagatatttt aacttcattt gtccattgtt caacagctag ttctgcatat
    16741 tcaggtcctt taactatcat tacataagat aatctaatgc ttagtataaa acacataatt
    16801 ataataaatg ctaggataaa cgaaagtctt tttcttatta tagccatatc tctatatgat
    16861 cttttcaata ttaaatacct cctctaaaaa aacttatcta tacataatat tagttaaata
    16921 agaaattaat tataaaattt aaaaaaaagc ttatttaaaa taaaagttta tttttatgtg
    16981 tatagtaaaa tttataataa tttaatgtta gtatttaaaa tattttgagg atggaataaa
    17041 aaatagaagt aataaaatgt aaattcttaa aaataaggtg gagattgaaa gaatatttgg
    17101 ttattattta catataaggt ttttacatac tatgatgttt tttaatattt taaaatatta
    17161 gctgaaatat tatgggctat attaaaaaat aaaaggggga agagtagagt tggagcataa
    17221 taaaattaag aatattaatc tatcaaagag aagaaaaaag atttttagaa gaagacttat
    17281 atttttaatt agtattatat tgattatttg tgggagtatt ttctttgttt tttctaagat
    17341 gaataaagat gagagttata gagatgctta taaaaaaaat atttcaagta gagagctaga
    17401 aaaaatgagg caggaattag atataaaaga agttaattat aaatggggaa gtgggcttaa
    17461 aaaagggaat tcacctaaaa gattaattat tcatcattca gcaacggatt caccagaaac
    17521 tccagaagat attcataagt ttcatttaga taatggatgg agtggaatag gatatcattt
    17581 ttatataagg gaggatggga caatttataa aggtagagat gaaaatgtaa taggagctca
    17641 tgcaaaaaat gcaaattata atactttagg aatatgcatt gaaggaaatt ttgaaaaaga
    17701 gggtttaaaa gaggcgcaaa aaaattcact tgttaaacta ggaacttatt taagcttaaa
    17761 atatccaata aaagatatac ttccacacag ggaagttgtg gatactttat gtccaggcac
    17821 actttttcca atggataata taaaaagtag tattattgag gaaataaaaa atatatgatg
    17881 attatatagt gagaattaga ttttataaac tcaatactga attgacatga aaaagtttaa
    17941 atggtaattt ataggtataa tttaataaaa attcttcagg gcagggtgaa attccctacc
    18001 ggcggtaaag cccgcgagct agtttttagc atgattcggt gaaactccga ggccgacagt
    18061 aaagtctgga tgaaagaaga atatctaata agttatcttt ttattatgtt ttatatgcat
    18121 attaaattga ttgttttgct ttagaatctt tagctctgaa catttttttg ttcagagctt
    18181 tactttttat gctatttata tcggtattaa ttgttttgaa ttggttaaaa ttttaagatt
    18241 ctatttaaat aacttcaatt acttggataa ctaatgaaag ccctgggtta ataaaaattc
    18301 agtgatttta ttttttatta ggtacttcta aagaagaatt tggaaggaga agataacttg
    18361 gatgaatttt ttatgaaaaa agctttggaa ttagctagaa aaggagaggg gtatgtaaat
    18421 ccaaaccctt tggtaggagc tgttattgtt aagggtggag aaataatagg ggaaggatac
    18481 catgagtttt ttggaggaaa tcatgctgaa attaatgcta ttaatagttc tattaaatct
    18541 actgagggag caacaatata tgttacctta gaaccttgtt gtcactatgg taaaactcct
    18601 ccatgtgtag aggctataat taaaaataaa tttaagagag tagtagttgg aaccttagat
    18661 ctaaatcctt tagtatcagg aaagggtatt agaattttaa gagaggcagg tatagaggtt
    18721 aaggtaggag ttttagaaaa ggaatgtatt agattaaatg agatatttaa tttttatatt
    18781 aaggaaaata gaccttttat tgcacttaaa tgggcaatga ccttagatgg gaaaatagca
    18841 actaaggatt ttaaatcaaa atggataact aataaaaagt caagagagtt tgttcattcc
    18901 ttaagaaata aatatagttc aataatggtt ggaataaaca ctgttttaaa ggataatcca
    18961 gatttaagat gtagacttaa taaaaataaa gagttagaaa gaaagcattt taggatagta
    19021 cttgatagta aattaaaaat tcctttagat tcagaaatat taaaaaatca aagtgagtca
    19081 aaaaccttaa tttttacaac aaataaaaaa gataagatga aaaatgaaaa attaaaagat
    19141 aaaggtatag aggtaataga agtaaattta gatgaaggaa aaattaatat taatcaggtt
    19201 ttagagtgtt taaaagaaaa aaatattgat tcagttctta ttgaaggagg aggaaatttg
    19261 aattttactt ttttagaaaa taaaaaagta aataagatat atgcctttgt tgcaccaaaa
    19321 gtttttggag ggaaaaatgc tattactcct gttgaagggg aaggaataag aaatgtttta
    19381 gacgcctttg aatttcctga agtaaatttg aagacctttc ataatgatat tttaattgaa
    19441 tgtagcctta gttaatagaa aaaggaagtg aataatatgt ttactggtat tgtggaagaa
    19501 ataggagaag ttataagcat agataaaaat gaaaaatcat ctttactgaa aataagagct
    19561 aataaggtct tagaggacac caagataggt gatagtatat caacaaatgg agtttgctta
    19621 actataacaa ctaagggaaa agattatttt actgcatatg ttatgggaga aaccttaaga
    19681 aaaagtaatc ttggaaactt aaaaaaatca agtaaggtga atttagaaag agccatgagt
    19741 attaatggca gatttaatgg acatatagta actggccata ttgattccac tggagaaata
    19801 atttctttta aagatgaggg ggaagcaata tgggtagaaa ttaaagtttc atatgaatta
    19861 agcaaatata tagtttataa aggctctata gccattgatg gaataagttt aactattgca
    19921 gaggttaaag gggaaagttt taaagtttca gtaattcctc atagccaaga agagacaaca
    19981 ttaactaaaa gaaaaattgg tgatttagta aatatagaat gtgatcctat tggtaagtat
    20041 gttgaaaagc ttttaggctt taaaaaagat agtagaaaga aaaagtctgt tttaacagaa
    20101 gagtttttaa ctattaatgg gtttatttaa attgtatttt aaaatttaat atttattttt
    20161 atatattgtt ttacatcaaa tttgatttta aaaagtagat ttattagtta caaaaaatat
    20221 ttaataattt taaaaattct tgaaaggtgg tgttggggat ttttatctcc ttaaaagtat
    20281 gaagaagttt aatttagtag aagaggcaat agaagatata aaaaatggga aaatggttat
    20341 tgttattgat gatgagggaa gagaaaatga gggggatcta gttatggctg gagaacttgt
    20401 tactggagaa aaaataaatt ttatggccac ttatggaaag ggtttaatat gtactccagt
    20461 aagtgagagt atagctgaaa atttagggtt gaatcctatg gtttcacata atacagataa
    20521 tcatgaaact gcctttacag tatctataga ttataaagat acaactacag gaatatcagc
    20581 ctttgaaagg gcttatacaa ttaaagaatt aataaatgaa gaaagcaagg cagaggattt
    20641 tagaagacca ggacatgtat ttcctttaat agctaagaat ggtggcgttt taaagagagc
    20701 tggacataca gaggcagcaa ttgacttagc aaagctagct ggattaaaag aagttggggt
    20761 aatatgtgaa ataatgaaag atgatggaac tatggctaga actgatgaat tattagaatt
    20821 tgcaaagatt cataatatga aaataataac tatagaagat ctaattgctt atagaacaga
    20881 aagagataat tttgtttata gagttgttga aactaatatg ccaactaaat atggagagtt
    20941 caaggctatt gggtttatag ataaacttac aggggagcat catgtagctt tagttaaagg
    21001 agagataaaa ccagaggagc cagtattatt aagagtacat tcagaatgct taactggaga
    21061 tgtttttgga tcaaggagat gtgattgtgg agagcaattt catagggcta tggaaaaaat
    21121 aaatgaagag ggaaaaggaa tattactata tatgaggcaa gaaggaagag gaataggtct
    21181 tataaataag ttgaaagctt ataagcttca agatgaggga ttagataccg tagaagctaa
    21241 tatagcctta ggatttccag aagatcttag agattatggc gttggagcac aaattttaaa
    21301 agagttaggc gtaaaggaaa tcaggttaat gagtaataac cctaaaaaaa taactggctt
    21361 agagggatat ggacttaaaa tagtagaaag agtacctata gaaataaagt gtaataagga
    21421 aaatgagttt tatttgaaaa ctaagaaaga gaaaatgcac catttaataa tgcagtaatt
    21481 ttatagagtt atttaaaact ttagatttaa agctagattt aaagtgtgta aaaaagtata
    21541 gatattagat acgaagaatt aaaaaaatac ttgaagtata aaaatgttta aaggagagat
    21601 ttgaatgaga attttagaag gaaatttaat aggtcaagat aaaaagtttg ctatagttgc
    21661 aggaagattt aatgaattca ttgtatcaaa gcttataggg ggagctttag atgcttttaa
    21721 aagacatgga gtagaggaag agaatataga tcttgcttgg gttccgggag cttttgaaat
    21781 accattaata gctaaaaagt tagctaaatc aggaaaatat gccggagttg tttgtttagg
    21841 aacagtaata agaggagcta ctagccatta tgattatgtt tgtggagagg tatcaaaagg
    21901 aatagctaat gtaagcttag atactgaagt accagttatc tttggaatag taacaactga
    21961 gaatatagaa caagctatag aaagagccgg aactaaagct ggtaataaag gatttgatgc
    22021 agctattgca gccattgaaa tggcaaatct tttagaaaat atataacttt ccaagttaag
    22081 catataagtt caagttataa aattaaagat tctataactt gaacttataa ttaaaagtat
    22141 ttatcttaaa attaagaata actatttaaa taaacttagt gttaaagttt gtttatttaa
    22201 cacataagtt acaaggatca gattttcctt tatttatagc ttcattaagg ctcatgctaa
    22261 ggatagaaga gcttcttttt aaggctgtac aatctttgct atagtgatag cttttaccgt
    22321 ttggagttac atatacaata gaatggtttt catcttttaa tggcgttgct ataccgttag
    22381 aatctatttt ccaaccatca ataattgtgt tagtaaccat ctcaccagaa ctatttaaat
    22441 agtaccaagt accattgtca tttagccagc cagtttgcat aactccgtta gaatttgaat
    22501 aataccattt agaatagact ttataccaac ctgtagccat agagccattg tcatttaaat
    22561 agtaccaagt accattgtca tttaaccaac cagtttgcat aactccatta gaatttgaat
    22621 aataccagtt agaatcaact ttataccaac ctgtagccat agaaccattg tcgtttaaat
    22681 agtaccaatt gccattatca tttagccagc cagtttgcat aactccgcta gaatttgaat
    22741 aataccagtt agaatcaact ttataccaac ctgtagccat agagccattt tcatttaaat
    22801 agtaccaaat accattatca tttagccacc cagtttgcat aattccattt ttatagaagt
    22861 accatattcc atttgtatta atccaacctt ctttagaaga tttaattaaa ttttcatttt
    22921 ttatgttata tgagttagat aaatttaagt ttgatacttg gattttcgaa ttaagtgttt
    22981 ctgcatttac attgattgta tatatgcttg aagatattag tccaagtgtt aatacaatcg
    23041 atgttatttt gtgattgatt ttcataatat tccccctaaa acaaatt
//
LOCUS       DAEVGI010000023        22994 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEVGI010000023 DAEVGI010000000
VERSION     DAEVGI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 22994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22994
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.023"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(44..904)
                     /locus_tag="K8M77_000852"
     CDS             complement(44..904)
                     /locus_tag="K8M77_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBI7085366.1"
                     /translation="MFIITYKMPKELVDEQIELLQINDIFNVYYESPLDITTDMFGYG
                     YKEKENVIVDLKVAFDGNLEDFENFKSQVSSLLKETPSSIDEVKNEFEEFYIDPIHLN
                     DQWVLCAPTDNFENKKEIFFTPQGAFGTGLHETTQDILKFIFEEDFEGKSLLDLGTGS
                     GILSIAAGVKGASKIVAVDIRDVEDEVLLNASLNELENIEVVVGNVLHEEYELDEKFD
                     WIFINIGGEETAMFMDFIEEHIEKTGKLLVSGLVEWSFDWVKEKVENKGFKLNKKFQT
                     NEWCTAIFSK"
     gene            1167..2924
                     /locus_tag="K8M77_000853"
     CDS             1167..2924
                     /locus_tag="K8M77_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HBI7085367.1"
                     /translation="MKKNAAQREYKKLSGQINQIENIVRHSKAGALFEHESTLRKKIL
                     QLKEMKDQGLKGYESLYDRYEELLEEVGKRILENYNKKNDTNFDFYEVLRNNYNVFLN
                     SGIMTLLVKHHIPELISKEFDEKFPANPKDEYLHTRRLKRKFYLHLGETNTGKTYTAM
                     QRLKEVRKGVYLSPLRILALENFERLNNEGIKCNLLTGEEEILFEDATHVSCTIEKAN
                     IHEKYDVAVIDEIQMIDDSQRGYAWTRALLGLYCTEIHICGAFNAKNILKEIIEDCGD
                     DYEIIEYHRDIPLIVEDESFHPKNVQEGDALVLFSKKKVLQMAEQYSQMGIKCSIIYG
                     DLPPEVRKKQYEEFITGKNKILITTDAIGMGVNLPIKRIIFLSISKFDGEQMRELTSQ
                     EVKQIAGRAGRKGIYDTGYVATYRDNKEFIEERLEEEDISIKRAVLGPSDAILEIDNL
                     PLNEKLALWSTKKCEVPYYRKMDISEYLIILERLKSYKLLEEIQWELLKIPFDISKDD
                     LMNQFLNFVDQLFINDQEELFKPQCYSGTLYDLETYYQMVNMYYSFSKRFNLNFDLEW
                     IENERLTVSEEINNILMRI"
     gene            2936..3226
                     /locus_tag="K8M77_000854"
     CDS             2936..3226
                     /locus_tag="K8M77_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HBI7085368.1"
                     /translation="MRVLNLKITLRASWVHSLKEKRMVVKSIVQRLKNKFNVSVGEVH
                     EQDIHQIIVIGISAVCGDQKQVDSTLENLIDFVEENTDAEIINIESDNYIFK"
     gene            3359..3754
                     /locus_tag="K8M77_000855"
     CDS             3359..3754
                     /locus_tag="K8M77_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085369.1"
                     /translation="MNTYEAPENNYILVMPDGSVVGFEESDMAQAYINRYYQEKITSY
                     CDKVDYSEYDMTEWKSTENICTALGAYEGLCILYKLEDLISSIQQSSIFEDEKNELIA
                     KLRCKNIDININDYQIDDILSNIDGVIYQ"
     gene            complement(4322..5101)
                     /locus_tag="K8M77_000856"
     CDS             complement(4322..5101)
                     /locus_tag="K8M77_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478555.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBI7085370.1"
                     /translation="MKKNLIFYFSTSGNSLYTAKEIAKHLKDAEIISIVDAFYKKEFK
                     YEAETIGFVYPIYASGLPPMVSEFLSKLQLNKDSYIFAAECTGGGKGITFSMINKLLY
                     PKNLSLSNGEKFLLNSNYIRMGRNPKVGEDAKKVIDSNKFKIENFTLSILNRESKNLK
                     EKHTRINLLLYKLWKDGFKNKDKNFNTNEFCTACGICAKICPNKNIEIIDESPKWKGN
                     CCDCMGCVNNCPHKCINIGNKTKKKNRYKNPYILTSELLRK"
     gene            5274..6782
                     /locus_tag="K8M77_000857"
     CDS             5274..6782
                     /locus_tag="K8M77_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455090.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI7085371.1"
                     /translation="MKKKLMLLFLTISILMMVLVGCSTNSNNIQKNSTNKEKITVATS
                     GTYYPFTFLEGGELKGFEVDVWNEIGKRLGYDVEFKTASFSGLFGMLESGKVNTIANQ
                     ITVTPEREEKYYFSEPYVYSGAQIIVKKGNDSIKSFDDLKGKKVGVDLGSNYEKIVKD
                     KDENNEIKILTYQNTDAAFNELLLGRIDAVVIDRVSAIAAIKEKDLNLQLAGEPIDKI
                     ENAYPFVKDEKGKDLSENVNKALDEMKKDGTLKSISEKWLGMNVSVPNNQENGNNIID
                     NNKNNSIGFDFIYSLDLIPMLLKAINVTISLSVFGMILGLIVGIALAMIRVYKIPVLK
                     QIAEVYISFFRGTPLLVQLFLLYFGVPQVIPSLQNMNAFTAALIGLGLNASAYIAEIL
                     RSSIDAIDKGQMEACLSLGMTRAQALKRVVLPQAFRIAIPPLGNIFVDTVKGSSLAFT
                     LGVVELLAKAQMEAAASYKFFESYVVVAIMYWIIIGFFNYLQKILEKKLSVY"
     gene            6801..7538
                     /locus_tag="K8M77_000858"
     CDS             6801..7538
                     /locus_tag="K8M77_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI7085372.1"
                     /translation="MIKVKNLKKSYKDLEVLKDVNMEVKQGEVVVIIGPSGSGKSTFL
                     RCLNYLESPDKGEIQVGHVKVNAEKISKKEINDLRKQSAMVFQHYNLFNNKTVLQNVT
                     EALIVVEKMDKKKAEEIALENLKKVGMVDKKDNYPNTLSGGQKQRVSIARAMAINPNV
                     TLFDEPTSALDPELVAEVLAVIKDLAKEHKTMIIVTHEMSFARDVADRVVFMDGGIVL
                     EEGTPEEIFKNPKNERTKQFLERFIVN"
     gene            complement(7585..7890)
                     /locus_tag="K8M77_000859"
     CDS             complement(7585..7890)
                     /locus_tag="K8M77_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085373.1"
                     /translation="MQEKVLRKLFLGFIHIHILHHAKVEPFFGSKMIEELREHGYSMS
                     PGTLYPILHNMERDGLLLRKDRLIDGKIRKYYSITSEGNDILEEAKSKAFELFKEIK"
     gene            complement(8250..9587)
                     /locus_tag="K8M77_000860"
     CDS             complement(8250..9587)
                     /locus_tag="K8M77_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBI7085374.1"
                     /translation="MYKFVEINSSEIDKFNESDRKGHIFQTSYWAELKKDWKKKFIAG
                     YDNDNNIVITATILLRKAPYINKYMGYIPRSFTCDYNNKKLLIEFTEYLREFAKKNNI
                     SFITIDPDIHLKENEEALSEGTEIKNFLKSLGYKNTDSKNFEAIQPNFVFRLPLPTEG
                     NKMDIKKAVFKKFSSKTRYNIKVAEERGLSVEVYDKETLNEDVLDRFHEIMVTTGKRD
                     NFLVRHREYFKDMIDYLYPHCRLYMVKYSYENDFNRLSEKLNKQEEAKTKAINKIEEL
                     KVKLDAETDEDKKSRIEKKLNDQDKRLKEAERQIEGFKKKISDIEPFKGQEIYLSGSI
                     YLYYGNKAWYLYGASENILRDTMPNFAMQWSMICDSIDLGCDVYDFRGVSGDLNPENP
                     LYGLYKFKKGFNGNFVEFIGEFDIVVDNGIYTLYKKAFPQFKKIRNAIMNKKQ"
     gene            complement(9853..10530)
                     /locus_tag="K8M77_000861"
     CDS             complement(9853..10530)
                     /locus_tag="K8M77_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBI7085375.1"
                     /translation="MSEFFNLEKLLTFFNYIFWFFMLNIFFSILNIPVILFFLFVGLS
                     NITTYLPLFLVSLIPFGASFTALLYCMRKLIKYKDLDLFSDFIKGLKYNFKQSTLMWC
                     GELFLILLLSFNLRIFSTVYYSLPLTCVFIVLMVLTLLTTTYIFVLISRFSMSSMDIL
                     RASVILTFTKPAVSIGNIVIFLFSLMLFEISPGTTVIFMASIVSYLIMFLNKNLLESL
                     ETKSSQA"
     gene            10752..12488
                     /locus_tag="K8M77_000862"
     CDS             10752..12488
                     /locus_tag="K8M77_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7085376.1"
                     /translation="MKFSKSNLYKRLFVTYTIVILSVVSILCIYFLSKTSEEIKRNNL
                     YLNDKILQDVNETLEYNSSTSRRIMNMLYEDDFRLNDVIKYLNSDLVDYIKGKLDYYS
                     ESDKLYYNGIESFVKSSFQLNNFLKSISFISYSRNEISVFNRKGEIKTYPFNSNELLD
                     KKDIEYAIYNKNEISIINEIRDAASLKPEGILMTTYDIGIISDVIKKYKDTNSEVLIL
                     NKEGMILYDSENKYDYEYYPYFDKLKVGRHEILLEDSYYVDILPDNNGLMSLVRIKVS
                     NATKVPILFKVSALLVSLLLFIIAECVLYIKFKNLSSRMDSLVLAMEKVEKGDIDVHI
                     PLSKDRDEITFIADNFNKMCEKLNDHIRKSYLAELRQREAEIVALQNQINPHFLYNTL
                     ESIRMKAICNGDKEVGKMLYSLAFLFRSQVKGNLIVSIDQEIKYCNKYLELFKFRYDE
                     KFKFKIECEEDLYDKGIVKFTLQPLIENYFVHGIRLERDDNELKIYIYKHLEDIVVEV
                     IDNGRGITKEKLDDINRRIRECDHSGKSIGMLNVHERIKIKYGEPYGLTVTSEENKGT
                     NMILKFPLREVE"
     gene            12493..14016
                     /locus_tag="K8M77_000863"
     CDS             12493..14016
                     /locus_tag="K8M77_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085377.1"
                     /translation="MYKVMLADDENLILQGLENIIEWEELGLEIVNKASNGQEAIDKF
                     KENPVDIVLTDINMPQVTGLELLKELKKINSDVKFIILSGYDDFSYAKKAIELGVENY
                     ILKPIDEEELEKTLKNTINKIKQEKEENKSSLGKHNILIKLIKGKLDQGEIEENKECF
                     YMNLNSERYSLCIINTRSRYDSEEMLHNIVNVIKENTQNNFEIIYTLDEELILINSWD
                     EALSKKEIKKYYDKLKEQIINEYGIDVFLSVGEPICDLYKISSSYKEANNLKKYVLTL
                     GYNKCITTEDVEDINEKNINFSEVLDKLNKRIIAKDIEGAEKIIEETVEDKKLNPRNI
                     YDLSVKILFLLDGIVEEFKVEKQYTGNSLGEEIVALCSEDTREDIKTLLCSEIREVIE
                     LMHPTTIKYSPVIQQIISYVNENYYEEVSLKTLARKYNINTSYLGQVFTKEVGCSFSE
                     YLNKTKNMKAKDLILNTNMKINDIAKKVGYLDTSYFYRKFKKYYGVCPSTLRNIKNY"
     gene            14357..14848
                     /locus_tag="K8M77_000864"
     CDS             14357..14848
                     /locus_tag="K8M77_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBI7085378.1"
                     /translation="MYKLGIITSSDKGYSGERKDESGLVIANIAKEKGFKVEKYIVLP
                     DEREMLRDEMINMADNLGVDLILTTGGTGFSKRDVTPEATKDVIEKETLGIPEAIRSY
                     SMTITKRAMLSRATAGIRKDTLIVNMPGSPKACREVMDYILDDLKHGIDILKGDAREC
                     ARK"
     gene            14833..15603
                     /gene="modA"
                     /locus_tag="K8M77_000865"
     CDS             14833..15603
                     /gene="modA"
                     /locus_tag="K8M77_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7085379.1"
                     /translation="MRKKISLSIIMIIGMLSLLFVGCTNNKEEKTLTVSIAASLQKPM
                     EKIAANFEKENGVKIQYNVGGSGTLKKQISDGADVDLFFSANTKYAKELIDEGLVNKD
                     ESYKILNNQLVLIENNDYNKPINSLEDLKNVEGKIAIGEISTVPAGQYAKESLEKVGD
                     YKDIEDKLVFARSVTNVKTYVENGEANLGFVYKTDTLDLKNSKIVYEVPNDYHKPIEY
                     ELCLLKNSKNNKEAKKLIEYLKGEESKKIFSEYDFEVN"
     gene            15607..16263
                     /gene="modB"
                     /locus_tag="K8M77_000866"
     CDS             15607..16263
                     /gene="modB"
                     /locus_tag="K8M77_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBI7085380.1"
                     /translation="MIWEALLISLKVVLVSVFLSCTLGILLGYFMVEKNFKFKYIIET
                     IIIFPMFLPPSAVGYLILLILGKKGVIGEFLYNNFDMSIIFTWIGAVIVGIVVSIPIM
                     YQSLKTSLLSVNDDVKNAAREMGAINFKLYRLISLPLSKKGLYTGIILSIARVFGEFG
                     ATILVAGNIQGKTQTIPMAMYYAIENGDSGLANKILILIVLISVILTFSYNKIIKRIK
                     "
     gene            16302..16778
                     /gene="moaC"
                     /locus_tag="K8M77_000867"
     CDS             16302..16778
                     /gene="moaC"
                     /locus_tag="K8M77_000867"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBI7085381.1"
                     /translation="MDNKLTHFDNKGNAVMVDVSNKNETERIAIATGTVKASSETIEL
                     IKSDQIGKGDVLGVARVAGIMAMKNTSNLIPMCHPVMITGSSIDFEIDSEKNEIRITA
                     TSKVVHKTGVEMEALTGVSIAALTIYDMCKAVDKRMVIGDIHLVKKLGGKSGEFNF"
     gene            17049..17975
                     /locus_tag="K8M77_000868"
     CDS             17049..17975
                     /locus_tag="K8M77_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI7085382.1"
                     /translation="MKDRIKRFRNNKELLLLTIPGAIWFLVFAYLPMFGVIVAFKRWR
                     IHGGFFESLMNSKWVGFDNFKFLFKSSDAWLITKNTVLYNLVFIILGIVLPVTLAILL
                     NELLNKKLAKFYQSSMFLPYFLSWVVVSYCLYAFLSPEKGYVNGILQSMGGKGISWYT
                     EPKYWPFIIIFMSQWKAVGYGTVVYLASICGIDKSYYEAAMIDGASKFQQIKYITVPL
                     LKPVMIIMFITSIGGMFRGDLGLFYQLPKDSGALYPVTNVIDTYVYRGLMNLGDIGMS
                     SAASLYQSFVGLILIVTSNAIVRKVDEENAFF"
     gene            17993..18949
                     /locus_tag="K8M77_000869"
     CDS             17993..18949
                     /locus_tag="K8M77_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBI7085383.1"
                     /translation="MLETIKQKRLQKKAEPKEEVNKFNAVSKKSNFLLNVLFAILAFL
                     AIMPFIFVIAISLTNEQSLQLNGYRFIPEEWSLEAYKYIFSSGSQILTSYGVTITVTI
                     LGTLIGLAIMSMYGYALSRKSFAYRKFFTKVIFVPMLFSGGMVASYLVVTRFLGLKNT
                     IWALILPMCVSSFHIIILRTFFKTSVPDAIIESAKIDGASEWRLFLRIVLPISLPGIA
                     TIGLFLTLGYWNDWFNAMLYIDEPNLIPLQYLLIRLENSMEFLANNATSLGANAIEAT
                     KNMPKDTAKMAIVVISTLPIIFAYPFFQRYFVNGLTIGAVKE"
     gene            19265..22399
                     /locus_tag="K8M77_000870"
     CDS             19265..22399
                     /locus_tag="K8M77_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBI7085384.1"
                     /translation="MRFISERIRKTYEEIGRYVYSNTLNIDNYKFIAGKYDNIDEIKN
                     APEEGWIDFKTGDLWGEESHGWFKTSVKVPDEFSGQTIALSFHTFLGDWDNTTNPQFI
                     LYVNGEHIQGLDVNHQETILTHNAVAGEVYDIDLHAHIGRIEGKKSTLHGKLVTIDEE
                     ARKLYFNLSVPHIVATKLHEEDKRRIDMLTVLNDTINLIDLRRPFTKEYNESLKIANK
                     YIEENFYDKMCGHEDMVATCVGHTHIDVAWLWTVAQTREKVARSFSTVLKLMEEYPEY
                     IFMSSQPQLYKFLKEDHPKVYEKVKEKVREGVWEPEGAMWLEADCNVTSGESLIRQIL
                     HGKRFFKEEFNVDNEILWLPDVFGYSAALPQILKKSDVNYFMTTKISWNQINKMPYDT
                     FMWKGIDGTEILTHFITTTYPDQDPFKETMTTYCGNIHPGSIMGAWNRYQQKNINNDV
                     LVAFGFGDGGGGATYEMLENGKRLSKGIPGAPKVKIGKAKDYFKKLEKTVKDNNKLPK
                     WVGELYLEYHRGTYTSMGRNKRDNRVCENLYTSAEKFNSMAMLLGKNYPLADLNAAWE
                     TILLNQFHDILPGSSIKEVYDVTKEEYKDLIEKGNNFVGSALDNITSEINLKERSVVV
                     FNSLGFERDDIATFDVPEDIENPAVLDGEEEIICQRIDDGEKAIFFAKGIPANGYKSF
                     KIIESTNKEVENVSLNEKEAENDFLRIKFNEEGNIVSLFDKRSEREVLKEGTLGNRLQ
                     AFEDKPMEYSNWDIDIYYKEKMWNVDDVRSIEVVEEGPVRSTLRIERKFLESTIVQNI
                     YIYRDLDRIEFNSYVDWKEHEVLLKTSFPIDLNATEATYEIQYGNVKRPTHSNTSWDE
                     ARFEVCGQKWADLSEGDFGVSLLNNCKYGHDIQDGDMRLTLLKSGTHPWTETDQEEHF
                     FTYSIYPHMGTWKETHTVQKAYELNQPLYTKVCQKHEGILEDTLGLVKVNKDNIMIEV
                     IKKAEDTDHLIIRMYEFFNSRTKDTLEFSREIEEAFECNLMERNLEEAKFEGKKIEFT
                     INPFEIKTFKVRLK"
     gene            complement(22464..22958)
                     /locus_tag="K8M77_000871"
     CDS             complement(22464..22958)
                     /locus_tag="K8M77_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBI7085385.1"
                     /translation="MEKSAQIFCILEGILMAIIGILFFIKPMDSLLYFTILSGILIII
                     SGVFRIIKAFKSSRKGLYIFTGIISVLFGLMLCFVPLDSIDVLVVFYGSWALVNGVFL
                     LVGEFTYKSFGFNASTLYSILLIILGLLIIFEPIAFLIATPFIIGIYFIIIAVFEIYL
                     GFKL"
BASE COUNT         9028 a         2498 c         3726 g         7742 t
ORIGIN      
        1 tttattaaaa tataatttaa aaagttaaat aattaaattt agtttatttt gagaatattg
       61 cagtacacca ttcattagtc tggaattttt tatttaattt aaagccttta ttctcaactt
      121 tttctttaac ccaatcaaag ctccactcaa ctaagcctga aactagaagt tttccagttt
      181 tttctatgtg ttcttcaata aaatccataa acattgcagt ttcttctcct ccaatattta
      241 taaatatcca atcaaatttt tcatctaatt catattcttc atgaagaaca tttccaacta
      301 ctacttctat attttctagt tcatttaaag aagcatttaa aagaacttca tcttccacat
      361 ctcttatatc tacagctact atttttgaag caccttttac tccagcagct atagaaagta
      421 ttccagaacc tgttcctaaa tcaagaagag attttccttc aaaatcttct tcaaatatga
      481 attttaatat atcttgagta gtttcatgta atccagttcc aaaggcacct tgaggagtaa
      541 agaaaatttc ttttttattt tcaaagttat cagttggggc acataaaacc cattgatcat
      601 ttaaatgaat tggatcaata taaaattctt caaattcatt tttaacttca tctattgaag
      661 aaggtgtttc tttaagtaat gaagaaactt gagatttaaa gttttcaaag tcttctagat
      721 taccatcaaa ggctactttt aaatcaacaa taacattttc cttttcttta tagccatatc
      781 caaacatatc tgttgttatg tctagtggag actcataata aacattaaat atatcattga
      841 tttgaagtaa ttctatttgt tcatcaacaa gttcctttgg cattttataa gtaattataa
      901 acatatatat gtatctcctt atattaagtt ttaattatgt ataagaagat tatagcatat
      961 taaaattagt gaggagtttt atttaagatt aagctttcta taaaataaat atttactttt
     1021 ataaagggat taatattaaa atatattttg aagaaaataa gaaaagggag agaaaaattt
     1081 taactgtttg agatatttat agaaaaagat tacaatgaga atatataggt taaatcatta
     1141 tagaatttta actcggagga attttaatga aaaaaaatgc ggctcaaagg gagtataaaa
     1201 aattatcagg tcaaataaat caaatagaaa acatagtaag acattcaaaa gcaggagcac
     1261 tttttgaaca tgaatcaacc ttaagaaaaa aaatattgca attaaaagag atgaaagatc
     1321 aagggttaaa aggatatgaa tctctttatg atagatatga agaattatta gaagaggtag
     1381 gaaagagaat tctagaaaat tataataaga aaaatgacac taattttgat ttttatgaag
     1441 tattaagaaa caattacaat gtatttctta attcaggaat tatgactctt cttgtaaaac
     1501 atcatattcc agagctaata tctaaggaat ttgatgagaa gtttccagca aatccaaagg
     1561 atgaatatct tcatacaaga agacttaaaa gaaaattcta tcttcactta ggagaaacga
     1621 atactggaaa aacctatact gctatgcaaa gacttaaaga ggttagaaaa ggtgtatatt
     1681 tatctcctct tagaatttta gccttagaaa actttgaaag attaaataat gagggtatta
     1741 aatgtaatct tcttactgga gaagaagaaa tattatttga agatgcaaca catgtatcat
     1801 gtaccataga aaaagctaat atacatgaaa aatatgatgt ggcagttata gatgaaattc
     1861 aaatgataga tgattcacaa agagggtatg cttggactag agctttactt ggtttatatt
     1921 gtactgaaat acatatatgt ggagccttta atgctaagaa tatattaaaa gagattatag
     1981 aagattgtgg agacgactat gagattatag aatatcatag agatattcca cttattgtag
     2041 aagatgaaag ttttcatcct aaaaatgttc aagagggaga tgccttagtt ttattttcaa
     2101 agaaaaaagt tcttcaaatg gctgagcaat attcacagat gggaattaaa tgctccataa
     2161 tctatggaga tttaccacca gaggttagaa aaaaacagta tgaagaattt ataacgggaa
     2221 aaaataaaat tttaataaca actgatgcca ttggaatggg agtcaatctt cctataaaga
     2281 gaattatttt cttatcaata agcaaatttg atggagaaca aatgagagag ttaacttctc
     2341 aggaggttaa acagattgca ggaagagcag gaagaaaagg catatatgat actggatatg
     2401 tagctactta tagagataat aaggaattta ttgaagagag attagaggaa gaggatatta
     2461 gtataaaaag agcagtttta ggaccttcag atgcaatatt agagattgat aatcttcctt
     2521 taaatgaaaa attagcttta tggagcacaa agaaatgtga agttccatac tatagaaaaa
     2581 tggatataag tgaatattta ataatattag aaagattaaa atcatataaa cttcttgaag
     2641 aaattcaatg ggagctttta aaaattcctt ttgatatatc taaagatgac ttaatgaatc
     2701 aatttttaaa ttttgttgat cagctattta taaatgatca agaagaacta tttaaacctc
     2761 aatgttattc aggaacttta tatgacttag aaacttatta tcaaatggta aatatgtatt
     2821 attcttttag caagagattt aatttaaatt ttgacttaga gtggattgaa aatgaaaggc
     2881 ttactgtgag tgaagaaata aacaatattc ttatgagaat ttaataggtg attttatgag
     2941 agttttaaat ttaaaaataa ctttaagagc atcttgggta cattccttaa aagaaaaaag
     3001 aatggtggtt aagagcatag ttcaaagatt aaaaaataaa tttaatgttt ctgtaggaga
     3061 ggttcatgaa caagatattc atcaaattat agttatagga atttcagcag tttgtggaga
     3121 ccaaaagcaa gttgattcaa ccttagagaa tctaatagat tttgtagaag aaaatacaga
     3181 tgcagaaatt ataaatatag aaagcgataa ttatatattt aaataaagtt aaaaaactaa
     3241 atttcaaact tataatatat gctgaaaatg gaaaagtata ttccaaaact atataagtat
     3301 aaggctttta ggtgaggaga aactaaaata aaataaacaa gaataggagg agattagtat
     3361 gaatacatac gaagcaccag aaaataacta tattttagtt atgcctgacg gaagtgtagt
     3421 aggctttgaa gaaagtgata tggcacaggc atacataaac agatattatc aagaaaaaat
     3481 cacttcttat tgtgataaag tagattattc agaatatgat atgacagagt ggaaatcaac
     3541 agagaatata tgtacagctt taggagcata tgaaggttta tgcattttat ataagctaga
     3601 agaccttata tctagtattc agcaatcatc aatatttgaa gatgaaaaga atgaattaat
     3661 agcaaaatta agatgtaaaa atatagatat aaatataaat gattatcaaa ttgatgatat
     3721 attatctaat attgatggag taatatatca ataaaaatat aaaatattaa aatttattta
     3781 tcaaattata gtatataaat tcataaaaag gataatataa taatgtaacg tggaggagat
     3841 aacttaaaga tttctaccga aagttacatt taaaaaattt tagtgtatgg cagggtaaga
     3901 attatttttc ttacggaagt ggcgtaacta aaattttaga atataaatct taatcagaag
     3961 agataattac ttttactgaa ctaagattaa atcttctaat aaaaaagctc tttgatatct
     4021 tatagtaatc ttagagtttt atcttaaata aaggtattgg gtaatttaat ttacatgagt
     4081 ttgcttaagt tattaggtat ttttatttaa gagaagggat aagttgtttg ataacttatc
     4141 cctttttaat ttttaaagtt attttaaact taatactcac ttaaaataaa tataaattaa
     4201 taaaaatttt tgtgatgaaa atttaaatat taattacttt aattttttgt agtttttatt
     4261 ctttattaag aaattttctt agccttatct agaaatcaat atgagcttaa gatagaattt
     4321 cttactttct aagaagctca gaggttaata tatatggatt tttatatcta ttcttctttt
     4381 tagttttatt tcctatattt atgcacttat gaggacagtt atttacacat cccatacaat
     4441 cacaacaatt tcctttccat ttaggacttt catctataat ttctatattt ttatttggac
     4501 agattttagc acaaattcca caagcagtac aaaattcatt ggtgttaaaa ttcttatctt
     4561 tatttttaaa accatctttc caaagtttat ataaaagtaa attaattcta gtatgttttt
     4621 cctttaagtt tttagactcc ctatttaata ttgaaagagt aaaattttct attttaaact
     4681 tgtttgaatc aattactttt tttgcatctt ctcctacctt aggatttcta cccattctta
     4741 tatagtttga gttaagtaaa aacttttccc cattagataa acttaagttc ttaggataaa
     4801 gtaatttatt tatcattgag aaggttatgc cttttccgcc accggtacat tcagctgcaa
     4861 aaatatatga atccttattt aattgtaatt tagagaggaa ttcacttacc ataggaggaa
     4921 ggccagaggc ataaatagga tatacaaagc ctatggtttc tgcttcatat ttaaactctt
     4981 ttttatagaa agcatctact atggagatta tttctgcatc ctttagatgt ttagctattt
     5041 ccttagctgt gtaaagtgaa ttacctgagg ttgaaaaata aaatattaaa tttttcttca
     5101 tataatatcc atccttatta aaaaataatt cattatgttc attttaatat taaaaataca
     5161 taatttctat ataaagacta aatataatta atacgaaata aaaatcaaag ttgacaattt
     5221 tgatgggaat taatataatt ggaactatat taaaatggaa agaggtcatg ctaatgaaga
     5281 aaaaattaat gttattattt ctaacaataa gtattttaat gatggtatta gttggttgca
     5341 gcactaatag taataacatt caaaaaaata gcactaataa agaaaagata acagtagcaa
     5401 cttcaggaac atattatcca tttacttttc tagagggtgg agaacttaag gggtttgaag
     5461 ttgatgtatg gaatgaaata ggaaagagat tagggtatga tgttgagttt aaaacagctt
     5521 ctttcagcgg attatttgga atgcttgaaa gtggaaaggt taatacaata gctaatcaaa
     5581 taactgtaac tccagaaaga gaagaaaagt attatttctc agagccatat gtatatagtg
     5641 gggctcaaat aatagttaaa aaaggcaatg attcaattaa atcctttgac gatttaaagg
     5701 gcaaaaaggt tggagttgat cttggaagta actatgagaa aatagttaaa gataaagatg
     5761 aaaataatga gataaaaata ttaacttatc aaaatacaga tgctgctttc aatgaacttt
     5821 tacttggaag aatagatgct gtagtaatag atagggtttc agctatagct gccataaaag
     5881 aaaaagattt aaatttacag ctagctggag aaccaataga taaaatagaa aatgcctatc
     5941 cttttgttaa ggatgaaaaa ggcaaagatt tatcagaaaa tgttaataaa gctttagatg
     6001 aaatgaagaa agatggaact cttaaatcta tttctgaaaa atggctagga atgaatgtaa
     6061 gtgttcctaa taaccaagaa aatggtaata atattataga taataataaa aataatagta
     6121 taggttttga ttttatatat tcattagatt taatacctat gttattaaag gctattaacg
     6181 taacaatatc tttatcagta tttggcatga tattaggatt aatagttggt atagctcttg
     6241 ctatgataag ggtttacaaa attcctgtac taaagcaaat agctgaggtt tacatatcat
     6301 tctttagagg aacacctctt ttagtacaat tatttttact ttattttggt gtgccacagg
     6361 taataccttc acttcaaaat atgaatgcat ttactgcggc attaataggt ttaggactta
     6421 atgcatcagc ttacatagca gaaatattaa gatcatcaat agatgccata gataaagggc
     6481 aaatggaagc atgtctatca ttaggtatga caagggctca agctttaaaa agagtagttt
     6541 taccacaagc atttagaata gcaatacctc ctttaggtaa tatatttgta gatacagtaa
     6601 aaggatcttc attagcattt actttaggag ttgtagaatt attagctaag gctcaaatgg
     6661 aagcggcagc tagctataaa ttctttgaaa gttatgtggt agttgcaatt atgtactgga
     6721 taatcatagg cttctttaat tacctacaaa agattttaga gaaaaaactt tcagtttatt
     6781 aagaggggag gagagaagtc atgataaagg ttaaaaatct taaaaagagc tataaagatt
     6841 tagaagtttt aaaagatgtt aacatggaag ttaaacaggg tgaggtagtt gttataatag
     6901 gaccttcagg ttcaggtaag tcaacttttc taagatgttt aaattattta gaatcaccag
     6961 ataagggaga aatccaagtt ggtcacgtga aagtaaatgc tgaaaaaata agtaaaaaag
     7021 aaattaatga cttaagaaaa caatcagcaa tggtatttca gcattataat ttgttcaata
     7081 ataaaacagt tctacaaaat gttacggaag cattgattgt agtagaaaaa atggataaga
     7141 aaaaggcaga agaaattgcc ttagaaaatt taaagaaggt aggtatggtt gataaaaagg
     7201 ataattatcc aaatacctta tcaggaggac aaaaacaaag ggttagtata gcgagagcta
     7261 tggctataaa tcccaatgtt actctttttg atgaacctac atcagcctta gatccagaat
     7321 tggtagcaga agtattagct gttataaaag atttggctaa ggaacataaa acaatgataa
     7381 tagttaccca tgaaatgagt tttgcaaggg atgtagcaga tagagttgta tttatggatg
     7441 gaggaatagt tttagaggaa ggtactccag aggaaatttt taaaaatcct aaaaatgaaa
     7501 gaacaaaaca gtttcttgaa agatttatag ttaattaaaa aaaactatag tggtatagaa
     7561 atttaatata taccactata gtttttattt tatttcttta aataattcaa atgctttact
     7621 tttagcttct tctaatatat cattaccttc tgatgtaata ctataatatt ttcttatctt
     7681 accatctatt aatctatctt ttcttagtag taaaccatct ctctccatat tatgaagtat
     7741 tgggtataaa gttcctggac tcatagaata tccatgttcc ctaagttctt caatcatttt
     7801 tgatccgaaa aaaggttcaa cttttgcatg gtgtaagata tggatatgta taaaacctaa
     7861 aaataatttt cttaaaactt tttcttgcat ggggtcatct cctataacaa ataaatatat
     7921 ataaatacga tatcgaaaat agattactgt taatgtttcc tattatatta tactccttaa
     7981 ttctttcaaa tcaaaagatt aatatatggt attatgctta aaatttaatg taaaatggtc
     8041 ttgtagttta attagattat ataaagttaa ctataaggag tatttgttat aaatagttaa
     8101 aatattgatt aaaaggtagg gttatactag ggaaaagaat gaaattagga aataaaaatt
     8161 atttaatttt aagtatgtat agaagctttt ttagagaaat tttttatata aagtttttaa
     8221 ataaaaaaag aatgcatatg cattcttttt tattgtttct tattcattat agcatttctt
     8281 atttttttga attgtgggaa agctttttta taaagtgtgt atattccatt atcaacaaca
     8341 atatcaaact ctcctataaa ttctacgaag tttccgttaa atcctttttt gaatttataa
     8401 agtccatata atggattttc aggatttaag tctccagaaa cacctctaaa gtcatataca
     8461 tcacaaccta gatcaataga gtcacaaatc attgaccatt gcatagcaaa gttaggcatt
     8521 gtatctctta atatattttc tgaagcaccg taaagatacc atgctttgtt tccgtagtat
     8581 aagtatattg atcctgaaag atatatttct tgacctttga aaggttctat atcagatatt
     8641 ttctttttaa agccttctat ttgtctttca gcttctttaa gtcttttatc ttggtcattt
     8701 aatttctttt caattcttga tttcttatct tcatcagttt cagcatcaag ctttacttta
     8761 agctcttcta ttttgttaat ggccttagtt ttagcttctt cttgcttgtt taatttttct
     8821 gataatctgt tgaagtcatt ttcgtagcta tacttaacca tgtataatct acaatgtgga
     8881 tataagtaat ctatcatgtc cttaaaatat tctctatgtc ttactaagaa gttatctctc
     8941 ttaccagttg taaccatgat ttcatggaat ctgtctaata catcttcatt taaagtttct
     9001 ttatcataaa cttcaactga taatcctctt tcctcagcaa cttttatgtt atatctagtt
     9061 ttactagaga attttttaaa tacagccttc ttaatatcca ttttattccc ttcagttgga
     9121 agtggaagtc taaatacaaa gtttggttgt atagcttcaa agtttttact atctgtgttt
     9181 ttgtaaccta aactcttaag gaagttcttt atttcagtac cttctgaaag agcttcttca
     9241 ttttctttta aatggatatc tggatctatt gttataaagc ttatattatt ctttttagca
     9301 aactctctta gatattcagt aaattctatt agtaattttt tattgttgta gtcacatgta
     9361 aaacttcttg gaatatatcc catatattta tttatgtatg gtgcttttct taaaagtata
     9421 gtagctgtga taacaatatt attatcatta tcatatcctg ctataaattt cttcttccaa
     9481 tcttttttta attcagccca ataagatgtt tggaatatgt gaccctttct atcagattca
     9541 ttaaatttat caatttctga tgaatttatt tctacaaatt tatacaattt agtttcctcc
     9601 ttaaacttca tcagttctaa acatatatat acattaagac ataataagta tattgtaata
     9661 tgaaataatt ttctgttcaa gtgtaacatt gctattatac aggaatatta gggctttatg
     9721 taaactttgt aaaaaataaa gatgctatat caaaagataa taataaaact tacaaatgta
     9781 tcaggaaatt actagagatt tcttaatact atttgtaagt ttaattaaaa gttattttga
     9841 tataacatct ttttaagctt gtgaactttt agtttctaaa ctttctaaaa gatttttatt
     9901 taaaaacatt attaaataag atactatgct tgccataaaa attactgttg ttccaggact
     9961 aatttcaaat agcataagtg aaaataaaaa tataactata tttccaatag aaactgctgg
    10021 tttagtaaaa gttaaaatta cactagctct taaaatatcc attgaactca tagagaatct
    10081 tgatattaat acaaaaatat atgttgtagt taatagtgtt aagaccatta atactataaa
    10141 gacacaagta agtggtaaac tatagtaaac agttgaaaat attcttaagt taaaacttaa
    10201 aagtaatatt aaaaataatt ctccacacca cattaaggta gattgtttaa aattatattt
    10261 taaaccctta ataaagtcac taaataaatc taaatctttg tattttatta attttctcat
    10321 gcagtaaagc agtgctgtaa aacttgctcc aaaaggaatt agagaaacta aaaataaagg
    10381 taaatatgtt gttatattac taagtcctac aaataagaag aacaagatta caggtatatt
    10441 taatatagag aaaaatatat taagcataaa aaaccaaaag atataattaa aaaaagtaag
    10501 tagtttttca agattaaaaa attcactcat ttttatcact ccataaaaca atgtaatggt
    10561 ttaactgatt tgtaattata gtttaagata agtagagggt aaaaacaatt taaatatgca
    10621 tgaattttaa atgaaaccat aaaaaataca taaaaacaat aaaatatcat aatttgataa
    10681 gcttttcata tagggtagaa tatgatttaa taaaaattga ctaaggtaat gtgggtgaag
    10741 aggggaatta aatgaagttt agtaagtcaa atctttataa gagactattt gtaacatata
    10801 caatagttat attatccgta gttagtattt tatgcattta cttcttaagt aaaacttctg
    10861 aagagattaa gagaaataat ctttatttaa atgataaaat actacaagat gtaaatgaaa
    10921 ccctagagta taatagcagc acatcaagaa gaataatgaa tatgctctat gaagatgact
    10981 ttaggttaaa tgatgttata aagtatttaa attcagattt agttgattat ataaaaggaa
    11041 aattagatta ttatagtgaa agtgataaac tttattataa tggtatagaa agctttgtta
    11101 agagttcatt tcaattaaac aactttttaa aaagtatatc ctttataagt tattctagaa
    11161 atgaaatatc agtttttaat agaaagggag aaataaagac ctatcctttt aattcaaatg
    11221 aattattaga caaaaaggat attgaatatg caatctataa taaaaatgaa attagtataa
    11281 taaatgaaat aagagatgca gcttctttaa agccagaagg aatacttatg actacttacg
    11341 atatagggat aattagtgat gttataaaaa aatataagga tacaaattca gaggttctta
    11401 tattaaataa agaaggaatg attttatatg attcagaaaa taaatatgat tatgagtatt
    11461 atccatactt tgataagctt aaagtagggc gtcatgagat tttattagaa gatagttatt
    11521 atgtagatat attaccagat aacaatggat taatgtcttt agtgagaata aaagtaagta
    11581 atgctacaaa agtacctatt ctttttaaag tttcagcact cttagttagt ttattattat
    11641 ttataattgc agagtgtgtt ctttatataa aatttaaaaa tttaagtagt agaatggatt
    11701 ctcttgtttt agccatggaa aaggttgaaa aaggagatat agatgttcat attcctttaa
    11761 gtaaggatag agatgaaata acatttattg cagataattt taataaaatg tgtgaaaagt
    11821 taaatgatca tataagaaag agttacttag cagaattacg tcaaagagag gctgaaattg
    11881 tagcccttca aaatcaaatt aatccacatt ttctttataa tacactagag tcaataagga
    11941 tgaaggcaat atgtaatgga gataaagaag tagggaaaat gctttatagc ttagcatttt
    12001 tatttagaag tcaagtaaag ggaaatctta tagtttcaat agatcaggaa ataaaatact
    12061 gtaataaata tttagagcta tttaagttta gatatgatga gaaatttaag tttaaaatag
    12121 aatgtgaaga ggatttatat gataagggaa ttgttaaatt tactcttcaa cctttaattg
    12181 aaaactattt tgttcatggt ataaggttag aaagagatga taatgaatta aaaatatata
    12241 tctataaaca tttagaagat atagttgtgg aagtaattga caatggtaga ggaattacta
    12301 aggagaaatt agatgatata aatagaagga ttagggaatg cgatcattct ggtaaatcaa
    12361 tagggatgct aaatgttcat gaaaggatta aaattaaata tggggaacct tacgggttaa
    12421 ctgttactag tgaggagaat aagggaacta atatgatttt aaaattcccg cttagagagg
    12481 tggagtagta atatgtacaa ggttatgcta gctgatgatg agaatttaat tttacaagga
    12541 cttgagaata taattgaatg ggaagaacta gggttagaaa ttgtaaataa ggcaagtaat
    12601 ggtcaagaag ccatagataa atttaaggaa aatccagttg atatagtgtt aactgatatt
    12661 aatatgccac aggttactgg gttagagtta ttaaaggaat taaagaaaat taattctgat
    12721 gttaagttta taatattaag tggatatgat gatttttctt atgctaagaa agcaatagaa
    12781 ttaggtgttg aaaattatat attaaagcct atagatgaag aagaattaga aaaaacttta
    12841 aaaaatacaa taaataaaat aaaacaagaa aaagaagaaa ataaatcaag tttaggaaaa
    12901 cataatattc ttattaaact tataaagggt aaattggatc aaggtgaaat agaggaaaac
    12961 aaagaatgct tttatatgaa tttaaattca gaaagatatt ctctatgtat cataaatact
    13021 agaagtagat atgatagtga ggaaatgtta cataacatag ttaatgtaat taaggaaaat
    13081 actcaaaata actttgagat aatatacacc ttagatgaag aacttatttt aataaattct
    13141 tgggatgaag ccttaagtaa aaaagagatt aaaaaatatt atgataagtt aaaagaacaa
    13201 ataataaatg aatatggaat agatgtattt ttaagtgttg gagaacctat ttgtgatctt
    13261 tataaaatta gttcaagtta taaggaagca aataatttaa aaaaatatgt tcttacttta
    13321 ggatataata agtgtataac aacagaggat gttgaagata taaatgagaa gaatataaac
    13381 tttagtgagg ttttagataa attaaataaa agaataattg ctaaagatat agaaggggca
    13441 gaaaaaatca ttgaggaaac tgttgaggat aaaaagttaa atccaagaaa tatatatgat
    13501 ttatctgtga aaatactatt tttattagat ggtattgtgg aagaatttaa agttgaaaaa
    13561 caatatacag gaaatagctt aggagaggaa atagttgctc tttgtagtga agatacaagg
    13621 gaagatatta aaactttatt atgtagtgaa attagggaag ttatagaact tatgcaccca
    13681 acaaccataa aatatagccc tgtaattcaa caaataataa gttatgtaaa tgaaaactat
    13741 tatgaagagg taagtcttaa gactttagct agaaaatata atataaatac ttcttattta
    13801 ggacaagtat ttactaaaga agttggatgt tcattttctg agtatttaaa taaaactaaa
    13861 aatatgaaag ctaaagatct tatattaaat acaaatatga agataaatga tatagctaaa
    13921 aaagtaggat atttagacac tagttatttc tatagaaagt ttaaaaagta ctatggagtt
    13981 tgtccatcaa ctttaagaaa tataaaaaat tactaattat aaagctatgg aatattaagc
    14041 tgtattcaaa gataaatttt gagtatagct ttttttacaa aactttgtta aaaaaataat
    14101 atttaaaaca agaataaatc aaataataat aaaacataat ataatattta attataactc
    14161 tccttaagtt ttatatatta tgtgataaaa atcacatttt ccaaagtttt ggaatgataa
    14221 aatttaaaca attgaaggaa gttattatat taataattgt tttaggtaaa tgttgatttc
    14281 tggctatata tttagaaacc aacaatagtt taatatagac ttttttatta aaattaaaag
    14341 gtaggtgctt tagagtatgt ataaattagg aataataact agcagtgata aaggatactc
    14401 aggagaaaga aaggatgaaa gtggattagt aatagctaat attgcaaagg aaaaaggatt
    14461 taaggtagaa aagtatatag ttttaccaga tgaaagagaa atgcttagag atgagatgat
    14521 taatatggct gataacttag gcgttgatct tatacttaca actggtggta ctggatttag
    14581 taaaagagat gttactccag aggcaactaa agacgttata gaaaaggaaa ccttaggaat
    14641 accagaagct ataagaagtt atagtatgac aataactaaa agagctatgc tttctagagc
    14701 tacagctggt ataagaaaag atactcttat tgttaatatg cctggaagtc caaaagcctg
    14761 tagagaagtt atggactata ttttagatga tttaaaacat ggaatagata ttctgaaggg
    14821 agatgctaga gaatgcgcaa gaaaataagc ctatctataa taatgataat aggaatgtta
    14881 tcattattat ttgtaggatg tactaataat aaggaagaga aaactttaac agttagcatt
    14941 gctgcaagtc ttcaaaaacc tatggaaaaa attgcggcta attttgaaaa ggaaaatgga
    15001 gtgaaaattc aatataatgt aggtggatca ggaaccttaa aaaaacaaat tagtgatggg
    15061 gctgatgtag atttattctt ctcagcaaat actaaatatg ctaaagagct tatagatgaa
    15121 ggccttgtaa ataaggatga atcctataag attttaaata accaattagt tttaattgaa
    15181 aacaatgatt acaataaacc tataaattct ttagaagact taaaaaatgt agagggcaaa
    15241 attgcaattg gtgaaatatc aacagttcca gcaggacagt atgctaaaga aagtttagaa
    15301 aaagtaggag attataaaga tattgaggat aaattagttt ttgctagaag tgtaacaaat
    15361 gttaagacat atgttgaaaa tggggaagct aatttaggat ttgtatataa gacagatact
    15421 ttagatttaa aaaatagtaa aatagtttat gaggtaccta atgattatca taaacctata
    15481 gaatatgaat tatgtctatt aaaaaatagt aaaaataata aagaagcaaa gaagcttatt
    15541 gagtacctaa aaggtgaaga aagtaaaaag atatttagtg agtatgattt tgaggtgaac
    15601 taaaaaatga tatgggaagc cttattaata tccttaaaag ttgtattagt atcagtgttt
    15661 ttaagctgca ctttaggaat attacttggc tattttatgg tggaaaaaaa ttttaagttt
    15721 aaatacatta ttgaaacgat aataatattt cctatgtttt tacctccttc tgctgtagga
    15781 tatttaattc ttttaatatt aggcaaaaag ggagtaatag gtgaatttct atacaataat
    15841 tttgatatga gtattatatt tacttggata ggtgctgtta tagtaggaat agttgtttca
    15901 atacctatta tgtatcagag tttaaagaca tccttactta gtgtcaatga tgatgtaaaa
    15961 aatgctgcta gggaaatggg tgcaataaac tttaaacttt ataggcttat aagtttacct
    16021 ctttctaaaa aaggattata tacagggatt atacttagta tagctagagt ttttggagaa
    16081 tttggagcta ctattttagt tgctgggaat attcaaggaa aaactcaaac aatacctatg
    16141 gctatgtatt atgctattga gaatggcgat tcaggtttag caaataagat attaatactt
    16201 atagttttaa tatcggtgat tttaactttt tcttataata aaataattaa gagaataaaa
    16261 tagaattttt aaagtttaga gaagaggaga gagtaacata tatggataat aaacttactc
    16321 attttgataa taaaggaaat gcagttatgg tagatgtaag caataaaaat gaaactgaaa
    16381 gaatagctat tgcaacaggt actgtaaagg catcaagtga aactatagaa ttaataaaat
    16441 cagatcaaat aggaaaggga gatgttttag gtgttgctag agttgctggt ataatggcaa
    16501 tgaaaaatac cagtaattta atacctatgt gtcatcctgt tatgattaca ggatctagta
    16561 ttgattttga aattgattct gaaaaaaatg aaataagaat aacagctact tcaaaagtag
    16621 ttcataaaac aggagttgaa atggaggctc ttacaggggt atctattgca gcccttacta
    16681 tttatgatat gtgtaaagcc gtagataaaa gaatggttat aggagatata cacctagtaa
    16741 aaaaactagg tggaaaaagt ggagaattta atttttaaat tattcatgtg aaaaagtgag
    16801 ttagagttta gcctctaact cttttttatt ataaaaataa aagtatataa attatatatt
    16861 ttatggactt tctattgtta ttaaactttt taatatttat aattgaaatt tatacagtaa
    16921 aactaaatat tttcaacttt tcccaaaata cataaaatga taagatttta ttgtgagaaa
    16981 cgtttacaac aaaaggggga agaagtgtga agagtaaagc taaaaagctt ccaaaagaga
    17041 agcttacaat gaaagataga ataaaacgtt ttagaaacaa taaagaatta ttactactca
    17101 ctataccagg ggcaatatgg tttttagtat ttgcttactt accaatgttt ggagtaattg
    17161 tagcattcaa aagatggaga attcatggag gattctttga aagtttaatg aatagtaaat
    17221 gggttggatt tgataacttt aaattcttat tcaaaagtag tgatgcttgg ttaataacta
    17281 aaaatacagt tttatataat cttgtattta taattttagg aatagtgctt ccagtaacat
    17341 tagcaatttt attaaatgaa ttattaaata aaaagttagc taagttctat caaagtagta
    17401 tgttcttacc ttatttctta tcatgggttg ttgtaagtta ttgtttatat gcattcttaa
    17461 gcccagaaaa aggatatgtt aatggtattt tacaatctat gggtggaaaa gggatttcat
    17521 ggtatacaga gccaaaatat tggccattta tcataatttt tatgagtcaa tggaaagcag
    17581 ttggatacgg aaccgttgtt tatttagctt caatatgtgg tatagataag agttactatg
    17641 aagcagcaat gatagatgga gcaagtaagt tccaacaaat aaaatatata acagttccat
    17701 tattaaaacc tgtaatgata ataatgttca taacttcaat tggtggtatg ttcagaggag
    17761 acttaggtct attctatcaa ttaccaaagg attcaggtgc tttatatcca gtaacaaacg
    17821 taatagatac ttatgtatat agaggtctta tgaacttagg agatattggt atgagttctg
    17881 cagcaagttt atatcaatca tttgtaggat taatacttat agtaacttct aacgctatag
    17941 taagaaaagt agatgaagaa aacgcattct tctaaaaaag gtggtgtaat gtgtgttaga
    18001 aactattaaa caaaaaagac ttcagaaaaa agctgaacca aaagaagaag taaacaagtt
    18061 taatgctgtc tcaaaaaaga gtaacttttt attaaatgtt ttattcgcaa tattagcttt
    18121 cttagcaata atgcccttca tatttgttat agctatatca ttaacaaatg agcaatcatt
    18181 acaattaaat ggatatagat tcataccaga agagtggagc ttagaagctt ataaatatat
    18241 attctcatca ggtagtcaga ttttaacatc atatggggtt acaattactg ttacaatttt
    18301 aggtacttta attggactag ctataatgtc aatgtatgga tatgctttat caagaaagag
    18361 ctttgcttac agaaagttct ttactaaagt aatatttgta ccaatgttat tctcaggtgg
    18421 tatggttgct tcatacctag tagttacaag atttttagga ttaaagaata caatatgggc
    18481 tcttatactt ccaatgtgtg taagttcatt ccatataatt attttaagaa cattctttaa
    18541 aacatcagta ccagacgcta ttatagaatc agctaaaata gatggagcat cagagtggag
    18601 attattctta agaatagtat taccaatctc tcttcctggt atagcaacaa taggattatt
    18661 cttaacttta ggatactgga atgactggtt caatgctatg ttatatattg atgaaccaaa
    18721 cttaatacca ttacagtact tattaataag acttgaaaac tcaatggagt tcttagcaaa
    18781 taatgcaaca tcacttggtg caaatgctat agaagctact aagaacatgc caaaagatac
    18841 tgctaagatg gctatagttg ttatttcaac tttaccaatt atatttgctt atccattttt
    18901 ccaaagatac tttgtaaatg gattaacaat tggagcagtt aaagagtaat taaatagaat
    18961 gatattttaa agggttgtat caatagcaac ttaaaataaa acttataaat atatttcgag
    19021 aattattgga tttcgctaag aatttatagt cattactccg aagaatttac taaagtttcg
    19081 aaacttgcga tctttcggac agtctcaact ttttaacgta aactcttcac gtaatggctt
    19141 aaattctaaa gcttatccaa atattctctt catactattt ataagtttta ttattttaag
    19201 ttttgataca accttttgtt aaagtttgag gtttgtagaa aataaggata aggagaaatt
    19261 tagtatgaga tttatatcag aaagaataag aaagacttat gaagaaatag gaagatatgt
    19321 ttattctaat actttaaaca tagataatta taaatttata gcaggaaagt atgataatat
    19381 agatgaaata aaaaatgcac ctgaagaagg gtggatagat tttaaaacag gggatctttg
    19441 gggagaggaa agtcatggat ggtttaaaac ttctgttaag gttcctgatg aattttcagg
    19501 acaaactatt gctttaagct ttcatacttt tttaggagat tgggataata ctacaaatcc
    19561 acagtttatc ctttatgtaa atggagaaca tattcaaggg ttagatgtaa atcatcagga
    19621 aacaatatta actcataatg ctgtggcagg agaagtttat gatatagatt tacatgcaca
    19681 tataggaaga attgagggca aaaaatcaac tttacatggt aagctagtaa caatagatga
    19741 agaagctaga aaattatact ttaacttaag tgttcctcac atagtagcaa ctaaattaca
    19801 tgaggaagat aagagaagaa tagacatgtt aactgtgtta aatgacacaa taaacttaat
    19861 tgatttaaga agaccattta caaaagagta caatgaaagc ctaaaaattg ctaataaata
    19921 tatagaagaa aatttctatg ataaaatgtg tggacatgaa gatatggtag ctacttgtgt
    19981 tggtcatact catatagatg tggcttggct ttggactgta gctcaaacaa gagaaaaggt
    20041 tgcaagaagt ttctcaacgg tattaaagct tatggaggaa tatccagaat atatattcat
    20101 gtcttctcaa cctcagcttt ataaattctt aaaagaagac catccaaagg tttatgagaa
    20161 agttaaagaa aaagtaagag aaggggtttg ggaaccagaa ggagcaatgt ggcttgaggc
    20221 tgattgtaat gtaacttcag gagaatcctt aataagacaa atattacatg gaaaaagatt
    20281 ctttaaagaa gaatttaatg tggataatga aatactttgg cttccagatg tttttggata
    20341 ttcagcagca cttcctcaaa tattaaagaa atctgatgtt aattatttta tgacaactaa
    20401 gatttcatgg aatcaaatta ataaaatgcc ttatgatact tttatgtgga aaggaataga
    20461 tggaacagaa atattaactc attttataac tacaacttac ccagatcaag atccatttaa
    20521 agaaactatg acaacatact gtggaaatat tcatccaggt tcaataatgg gagcatggaa
    20581 tagatatcag caaaaaaata taaataatga tgttttagtt gcctttggat ttggagatgg
    20641 tggcggtggt gctacttatg aaatgcttga aaatggaaag aggctttcta agggaatccc
    20701 aggagcacct aaggttaaaa taggtaaggc taaggattac tttaagaaat tagagaaaac
    20761 tgtaaaagat aataataaac ttccaaagtg ggttggagaa ttatatcttg agtatcatag
    20821 aggaacttat acttcaatgg gaagaaataa gagagataat agagtatgtg aaaatctata
    20881 tacttcagca gaaaaattta actcaatggc tatgttgtta ggaaaaaact atcctttagc
    20941 tgatttaaat gctgcttggg aaacaatact tttaaatcaa ttccatgata ttttaccagg
    21001 ttcatcaatt aaagaggttt atgatgtaac taaggaagaa tataaggatt taattgaaaa
    21061 aggaaataat tttgtaggtt cagctttaga taatataaca tcagaaataa acttaaagga
    21121 aagaagtgtt gttgtattta actctctagg atttgaaaga gatgatatag ctacttttga
    21181 tgttccagaa gatatagaaa atccagctgt attagatgga gaggaagaaa ttatttgtca
    21241 aagaatagat gatggagaaa aagcaatttt ctttgctaaa ggaatacctg caaatggata
    21301 taagagcttt aaaataatag aatctaccaa taaagaagta gagaatgtat ctctaaacga
    21361 aaaagaagct gaaaatgact tcttaagaat taaattcaat gaagaaggaa atatagtttc
    21421 attatttgat aagagatcag aaagagaagt attaaaagaa ggaactttag gaaatagact
    21481 tcaagctttt gaagataagc ctatggaata tagcaactgg gatatagata tatactacaa
    21541 agaaaaaatg tggaatgttg atgatgttcg aagtattgaa gtcgttgaag aaggcccagt
    21601 aagaagcaca ttaagaattg aaagaaaatt cttagagtct actatagttc aaaatattta
    21661 tatttataga gatttagata gaatagagtt taattcatat gttgattgga aagaacatga
    21721 agtattatta aaaacttcat tcccaataga tttaaatgct acagaggcta cttatgaaat
    21781 tcagtatggt aatgttaaga gaccaactca tagtaataca tcatgggatg aagctagatt
    21841 tgaagtttgt ggacaaaaat gggctgacct ttcagaggga gactttgggg tatccttatt
    21901 aaataactgt aagtatggcc atgatataca agatggagat atgaggctta ctttattaaa
    21961 atcaggaaca catccatgga ctgaaacaga tcaagaggag catttcttta cttattctat
    22021 ttaccctcac atgggtacat ggaaagagac tcatacagtt caaaaggctt atgaattaaa
    22081 tcaaccttta tatacaaagg tttgtcaaaa acatgaggga atactagaag atactttagg
    22141 cttagtaaaa gttaataaag ataatataat gattgaggtt attaaaaaag ctgaagatac
    22201 agatcattta ataattagaa tgtatgaatt ctttaatagt agaactaagg atactttaga
    22261 attctcaagg gaaatagaag aagcctttga atgtaactta atggagagaa atttagaaga
    22321 agctaaattt gaaggaaaaa aaatagagtt tactataaat ccatttgaaa taaaaacatt
    22381 taaggttaga ttaaaataat aaaaagaggc tatatcaaaa tgaaattagt tcatttttga
    22441 tatagcctct ttctatgttt attttatagc ttaaatccta agtaaatttc aaatacagct
    22501 attataataa agtaaattcc tattataaat ggtgtagcta ttaaaaaggc aattggttca
    22561 aatattatta atagtcctaa tattattaat aatatagaat agagtgttga tgcattaaaa
    22621 ccaaagcttt tataagtaaa ctcaccaaca agtaagaata caccattaac taatgcccaa
    22681 gaaccataga atacaactaa tacatcaata ctatctaatg gaacaaaaca tagcattaaa
    22741 ccaaataata ctgatattat acctgtaaaa atatataagc cttttcttga agatttaaaa
    22801 gctttaatta ttctaaatac acctgagatt attattaata tacctgatag tattgtgaaa
    22861 tataataatg aatccattgg tttaataaag aataagatac ctataattgc cattaaaatt
    22921 ccttctaata tgcaaaatat ttgtgcgctt ttttccattt aaatcactcc taaattttta
    22981 aaaagttttt atat
//
LOCUS       DAEVGI010000024        22746 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEVGI010000024 DAEVGI010000000
VERSION     DAEVGI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 22746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22746
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.024"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(17..571)
                     /locus_tag="K8M77_000872"
     CDS             complement(17..571)
                     /locus_tag="K8M77_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085386.1"
                     /translation="MTRNFKKTIWLLVGVMIFGIIGMVGCTKKTNEKTENSGLSATTP
                     AEYKTMLEEKIKTYIGDANLGTEYDFSKVTEDTKQATYEEYEKYYKNLDTELTNLKNE
                     LNSKVSPSDGKVNDANRKIVESIDNLKMSISTVKSDLDANRNKILAMPKDEFISAMKD
                     IEKSAYDARVKVQESIDAVKNSFK"
     gene            complement(744..1694)
                     /gene="whiA"
                     /locus_tag="K8M77_000873"
     CDS             complement(744..1694)
                     /gene="whiA"
                     /locus_tag="K8M77_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBI7085387.1"
                     /translation="MSFSAKVKGEICRYIDISKEEALAQISAIMKVCGTLAFSGRQIS
                     FKMTTENPASARLMFTILKDYFDIHAKLMVKKSNSLKKNNIYMVVVTEEMGVKKLLEI
                     TGILREIDGIMSLDYHIDENLVDTEEKKKAYIRGAFIGGGSISNPEKTYHLEFVTHSQ
                     EYAEDLGKLINTFGLKAKVIQRKNSYIVYIKEGEQIVDLLNIIGAHTALLELENIRIM
                     KEMRNNVNRLVNCETANLSKTVNAAVRQVESIKLIEREIGLARLPKNLREVAELRLTY
                     PEESLKELGEMLEPPVGKSGVNHRLRKIEKIAEELRTGNF"
     gene            complement(1697..3043)
                     /locus_tag="K8M77_000874"
     CDS             complement(1697..3043)
                     /locus_tag="K8M77_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBI7085388.1"
                     /translation="MMMKKMLKPGIKVKRYIFLALASIITMAVAIAKLIDERHVTTIH
                     DKAVFIILIIFSGLVGYKSIVWCVKSVVSLVNKGYINLSIEEDDLEDLIEDDRLLIKG
                     PKIVVIGGGTGLSIMLRGLKHYTSNVTAIVTVGDDGGGSGILREDLGILPPGDIRNCI
                     LALANTEPLMNELLQYRFNDGRLKGQSFGNLFLAAMDGISENFEDAVQKMSSVLAVKG
                     EVLPVTLENMVLEAELMNGHRVRGESLIGEEVIDQSSPIKKLKIIPEDAKALDRALEA
                     IEDADAIVLGPGSLYTSVLPNLLVKDISKAIKKSKAFKIYNCNIMTQPGETDGFKVSD
                     HVQVILDHCGKDMVDCIIANSKDISEELKEKYLDKNSELVELDINKLKKMGLCVVDGD
                     LTKVKGDYIRHNSDYLAQLLIETVMEKHLLYDRKKILEYIYLSQRIKKREGSNRKE"
     gene            complement(3040..3924)
                     /gene="rapZ"
                     /locus_tag="K8M77_000875"
     CDS             complement(3040..3924)
                     /gene="rapZ"
                     /locus_tag="K8M77_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBI7085389.1"
                     /translation="MRFVIVTGLSGAGKTEATRSLEDLGYFCVDNLPPKLIPKFAEAC
                     VQSEGKIDKIALVIDIRGGIFFDDLFESIEYLKANDFNYEILFLEASDEVLVKRFKET
                     RRSHPLSPDGRIITGISEERMRLRELKDRADNIIDTSNYPIRNLREKINLLYGDGKPV
                     EQNLSITVLSFGFKYGIPSDSDLVFDVRFIPNPFYIPELKPFSGEDEPVKNYVLAQEE
                     TKGFLSRLSDMAEFLIPNYIKEGKRQLIISIGCTGGRHRSVAIANALYKDLLCKNFHV
                     TLEHRDINEDINRGDRKL"
     gene            complement(4162..5076)
                     /gene="murB"
                     /locus_tag="K8M77_000876"
     CDS             complement(4162..5076)
                     /gene="murB"
                     /locus_tag="K8M77_000876"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBI7085390.1"
                     /translation="MNQYMEFYKLLGEFYNEEDITVDSPMSEHIYFRVGGPADILVTP
                     VNEEQVVNTLKLCREYNVPYFILGNGSNILVKDGGISGVVIKFNKLNKITTEGNCVTA
                     QSGALLKDVSKAALENNLRGFEFACGIPGSIGGAVFMNAGAYDGEMAHVIKSARVIDE
                     NCNIKNLTKEELELGYRSSIVMKKGYVVIEATIELESGEYASIKDKIDDLTNRRESKQ
                     PLEYPSAGSTFKRPEGYFAGKLIQDSGLKGFSIGGAAVSEKHSGFVINKGGATAKDVL
                     DVIAHVQKTVKENFDVELHTEVRIIGRD"
     gene            complement(5226..7088)
                     /gene="uvrC"
                     /locus_tag="K8M77_000877"
     CDS             complement(5226..7088)
                     /gene="uvrC"
                     /locus_tag="K8M77_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBI7085391.1"
                     /translation="MFDFQHQLKILPDKPGVYIMKNSLGEVIYVGKAKVLKNRVRQYF
                     QNSKNHSEKVRAMVKNIAEFEYIVTDSEMEALILECNLIKKYSPRYNIALKDDKFYPF
                     IKITTNEDFPRVYVTRNFAKDGNRYFGPYTNGTAVYEVMGLIKKLFPLRTCKKAIVEG
                     GEPTRACLNYHINLCKAPCAGYVSKAEYWKMIDEIINILNGTDTSIIKNLKLEMEKAA
                     EELEFEKAAKIRDRILAIELISEKQKMFTVKEGDEDFIDLYTDEKDGCAQVFFVREGK
                     VTGREHFMIENISDDPVKEVISSFIASFYGGTAQIPKTIYVPEEIEDQELIEKFLTEK
                     RGSKVWIKVPKKGDKKNLLDMVRNNAKIMLDQFKEKMVEEKELNKSALTELADVLGLD
                     SLPARIEAYDISNIQGVDSVGTMVVFENGKAKNSDYRRFKIKSVKGPNDYESMREILS
                     RRFSHGLEEVNKIKERNLEYSKGKFCIFPDLIMMDGGKGQVNIALEVLKDFGIEIPVC
                     GLVKDDKHRTRGIIFNNEEILIRRGSGLMNLITRVQDEVHRYAITYHRSLRDKRTLNS
                     ILEDIPRIGEKRRRNLLMKFGSIDNIKKASMEELLDTPGIDKRAAESIKQYFSS"
     gene            complement(7245..8708)
                     /locus_tag="K8M77_000878"
     CDS             complement(7245..8708)
                     /locus_tag="K8M77_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBI7085392.1"
                     /translation="MAKKKDLSGSIKKVMFVYLVLLVGLISYIAYFQLIQVPKIAEMD
                     SNSAVMAAQNEVIRGEIKDRDGNVLAKSTKSGDLTQKREYPYGEIYSHPIGYASGIYG
                     NAGLEGAYSKELTTYENVSLKAFLKSFNLKKDFKERNESDKKVGNSIVTTLDTDLQKA
                     AYAALGANRGAVVALDPKTGAVLASVSKPGFNPNNMKETYEIANNPNGNPKDAILVDR
                     ALNGQYPPGSTFKVVTLTSALENLPGVIERTFNDTGSINVGTKDLPNENGNAYGNISL
                     RKALSVSSNVIFGGILGEELGNKKLRETAEKYGFNKDLYLGNLKAYSGNFPDRKTIDK
                     GLIAYDAIGQGEVLASPIEMAMVAATVANNGVRMEPYIVSKVLDKDGDTVKEFSSTKK
                     DTVMSKEQAQIINEYMQGVAKDRINDNWGYFKGMNVAAKTGTAQVPDGNSHAWLIAFA
                     PADDPKIAVATIVENGGSGSKVAAPVTARVMQAYFNK"
     gene            complement(8709..9938)
                     /locus_tag="K8M77_000879"
     CDS             complement(8709..9938)
                     /locus_tag="K8M77_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBI7085393.1"
                     /translation="MNTTLKYEKRMLLLVYLLCLALFTNIAIKQTPIDKMAFIIAGIL
                     MLIIGFSHFIIRKFYPDGDKYMLIFAAVLAVVGIAVLYRLDPKLAIKQLIWFSLGIAI
                     YMGIVIVMPDLKSFAKYKYIYMGGTLIFMAMAMIIGRTVNGSKNWVYIGSFGFQPSEI
                     GKIFFILYLASALMKYEKKDNIKYEFKQLLEPALIVMYSLGFMVLQKDLGSALMFFFV
                     SITMLYIATCNWKYVGTGLVLFSLGGTASYFLFNHVKKRVMIWKDVWKYANNESYQIV
                     QGFYAMSLGGMFGTGLYNGYPKLVPFASTDFIFTLIAQELGLVFGIGLLLLYFLLFYR
                     GIRAALNTDDPFSQLNAVGFSTLIVAQVLVIIGGVFSVIPLTGITLPLVSYGGTSMLT
                     VFIALGILQKISEEGLR"
     gene            complement(9952..10386)
                     /locus_tag="K8M77_000880"
     CDS             complement(9952..10386)
                     /locus_tag="K8M77_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HBI7085394.1"
                     /translation="MFSKLMTWVFGIIFIIILYSIIYYALKIMYKDVKGGKKKKAPGK
                     KAHGLEVLKTISDGTLGIGSVIPVTSTISIGRREDNSIVLNDQFVSSYHAKIYVKNND
                     FYLEDLASTNGTFINDSKVEGRVRLKVNDQVRMGSTVFKVIG"
     gene            complement(10720..13539)
                     /gene="uvrA"
                     /locus_tag="K8M77_000881"
     CDS             complement(10720..13539)
                     /gene="uvrA"
                     /locus_tag="K8M77_000881"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBI7085395.1"
                     /translation="MKDKIIVKGAKVHNLKNVSLEIPRDKLIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGQMDKPDVESIEGLSPAISIDQKTTSRNPRSTVGTV
                     TEIYDYLRLLYARVGVPHCPKCGKEITQQSVDQIVDQIMELKERSKIMILAPIIRGRK
                     GTHEKVLENIKKQGFVRARIDGEIYDLTEDQIKLEKNIKHNIEAVVDRIIVKDGIEGR
                     LTDSIETSLKMAEGLVLVNIIGEEDRLYSEHFACADCGISIDELAPRMFSFNSPFGKC
                     ERCDGLGTLMEIDEDLVVPNKDLSIRGGAISTWGDSRMKEESWTYCVLKALMEKYNFD
                     LDTPYKDLPKKVQEVLMYGEPEKLKVTYTKENVTAVYNHSFEGEINNLRRRYMETNSD
                     TMKAEIEKYMSDNPCPKCKGARLKPEALAVTVGGKNIFEFTSMAIREELDFINSIDFS
                     EKDKIISSQIIKEIQSRLSFLINVGLDYLDLARKAGTLSGGEAQRIRLATQIGSQLMG
                     VLYILDEPSIGLHQRDNDRLISTLKQLRDVGNTLIVVEHDEDTMREADYIVDIGPGAG
                     EHGGEIVAKGTLDEIMRNENSLTGKYLTGAKKVELPEKRRKGNGNFITVKGAKENNLK
                     NVTTKFPLGTLTMVTGVSGSGKSTLVNEILYKGLNKIVNKAKDLPGKFKEITGYENID
                     KIIDIDQSPIGRTPRSNPATYTGTFDIIRELFSQTQEAKMRGYKPGRFSFNVKGGRCE
                     ACSGDGIIKIEMQFLSDVYVPCEVCKGKRYNRETLEVKYKGKNIADVLNMTVEEALEF
                     FENIPRIKNKLQTLMDVGLGYIRLGQPSTQLSGGEAQRIKLAYELSKRSTGKTLYILD
                     EPTTGLHIHDVNRLVKILQRLVDGGNTVIVIEHNLDMIKCADYIVDLGPEGGDKGGTI
                     IATGTPEKIAETKESYTGKYLKKYL"
     gene            complement(14055..16220)
                     /gene="recQ"
                     /locus_tag="K8M77_000882"
     CDS             complement(14055..16220)
                     /gene="recQ"
                     /locus_tag="K8M77_000882"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBI7085396.1"
                     /translation="MNRAKEILKSFYGYSEFRKGQEEIINEIISGNDVVAIMPTGGGK
                     SICYQIPALILEGVTVVISPLISLMKDQVDAINSMGIESAYINSTLSNEEINNIFQSV
                     KDGHIKILYVAPERLESMEFLNLVSNIDISQVAVDEAHCVSQWGHDFRSSYKFIGRFI
                     NILRKRPVVSAFTATATEEVRKDIVKLLELNNPKVFVSGFDRDNLKIIIEKGVNKAHY
                     ILDYLKENKDVSGIIYCSTRREVDSLCELINSRGIGCTKYHAGLSDEERKKNQEDFVF
                     DKVSVMVATNAFGMGIDKPNIRYVIHNNMPKNIEGYYQEIGRAGRDGEKSECILIFSP
                     GDVQTQKYIIETGTLNPERKINELSKLQTMMDLVYSNGCYRRFILNYFGEDLEEDCHN
                     CSNCEMEGELVDKTIDAQKVISCVYRMKRPFGIGVIIDVLRASKNKKIIELGLDQLST
                     YGIMKDYSKEGLKDFINTLISHRYIDYIGEYPVVRLNQSSIDILKGNEKVMFKEKIKA
                     KRVSKNNELFDLLRSLRREIASEEGVPPYIVFGDNTLKEMSVRIPLNESQLSEISGVG
                     EKKILKYGEMFLNVINEYVKVNNIEIKWESGLKINKDELEEKFKKGNGKDGKKKSFEI
                     TIDMLREGKKFHTIVKERELSITTVLSHIQQYLEEGNKIDFEVDFDSLFSKREEELVL
                     DAIDKVGYSKLKTIKENLEEYVSYEKIRFIVTKFIINMI"
     gene            complement(16341..17927)
                     /locus_tag="K8M77_000883"
     CDS             complement(16341..17927)
                     /locus_tag="K8M77_000883"
                     /EC_number="2.6.1.1"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate transaminase/aspartate
                     4-decarboxylase"
                     /protein_id="HBI7085397.1"
                     /translation="MITKDLEKKLEGLGAFEISSKMLKLAKNNEKHNKFLNAGRGNPN
                     WINTEGRLALGRIVEFGVFESRRTIHEGNLAGYTNLDGIYERLMDFLNGGHEVDKFLR
                     DAIDYSIKTLSVNKDELVKEFVDGAIGNDYPVPNRCLVNTEKILKLYLEKELFGGVKL
                     ANETNVFPTEGGTAAICYIFNSLKENGLIKAGDKIAINTPIFTPYLQIPELKDYEMVE
                     VDLISKEENNWVIEDSELDKLRNKEIKALFFVNPSNPASKALSEEALNHIADIVKERK
                     DLMIITDDVYGTFVENFKTIYSVAPHNTLLVYSFSKLYGVTGWRLGLIAANENNVFDE
                     LISKLTEEKLQDLDKRYGLVTFEPRKFKFIERIVADSRSVGLYHTSGLSTPQQIQMAL
                     FALTSLITKGRDPYIESSKKLVRDRYHDLCNTLGIKEDDSRENAKYYSLINIYDLARD
                     LYDVNFKEYLKNNFKPIDFLLRLAEINGVVLMEGVGFGADPGILRVSEANLPDEAYKK
                     IAKQILEMLSEYYNQYKLEK"
     gene            complement(18236..19585)
                     /locus_tag="K8M77_000884"
     CDS             complement(18236..19585)
                     /locus_tag="K8M77_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBI7085398.1"
                     /translation="MEFKDFKERLFNRAKELNFEKYELYYGKSEALNITSSNGEVERY
                     GLSTSIGVSFRGIYKGKMGYSYTEKLDDESIEILLLKAIECAELVESEDEEFIFGDKA
                     KYVEVNSYDENISKLTPKEQIEKALKLSTEPSKINFEIIKSEYTKFNTTKLERVIVNS
                     EGIDLKEEKTIVQASISPIAKDGENMIVDGVSRVSTKLNDVNIDELMKKAINNTLRKK
                     GATSIKSGKYKVIIDGEVMASLIRTMEDNFLGHIAQENKTLLKDKIGEKIASNKVTLV
                     DNPFLKGGISTCSFDDEGVPTRVKNIIENGVFKGFLHSLKTAKKSKVNPTGNGFKSGF
                     KSTVKASPTNLYIKEGEKSFEELLSYVENGVYIIDLAGLHSGANAVTGDFSLAAEGIK
                     IENGVLKGAIKHITIAGNFFDLLNNIEEVGNDLEESGVTSGYGSPSIIIKELSVAGE"
     gene            complement(19601..20986)
                     /locus_tag="K8M77_000885"
     CDS             complement(19601..20986)
                     /locus_tag="K8M77_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBI7085399.1"
                     /translation="MLSKDTIKKVLSVALSTGGDFAEVFVEEKLNNNIVLTSGAVKDI
                     ISGKDYGIGIRIFKGLKCIYAYTNDDSLMSLVNTAKKAALALGDVIDGHTVNLNLVER
                     INTNIHPILYVPSSISSAKKIEKMKEAYKGAKGYNHEISQVTVTMKDEDQRVLIANSE
                     GLFTEDRRIRSRIAISSIASNGVDNQAGTRSPGASMGFEFFDTIDLSNLGKEASKSAV
                     TMLHADYCPAGKMTVAIENSFGGVIFHEACGHSLEATAVAKGNSIFCDKLGMQIASKK
                     VTAIDDGTIANAWGSLNIDDEGEPTRRKVLIENGILKSYMIDKFNGRRMGMPSTGSGR
                     RESYKYAPTSRMTNTFIGNGEDDNEKIIKSIENGLYAKQMGGGSVNPITGDFNFNVLE
                     GYLVKNGVIDRAVRGASLIGNGADILMNIDMVGKNLDYGQGVCGSLSGAVPTNVGQPL
                     LRVKEITVGGR"
     gene            21259..22563
                     /locus_tag="K8M77_000886"
     CDS             21259..22563
                     /locus_tag="K8M77_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HBI7085400.1"
                     /translation="MSITKERFFNEFKKDQATYDEIHENILDGVNIGGANFIILMCAI
                     IIASVGLNMNATAVIIGAMLISPLMGPIIAIGYSVGIYNLKLLKKSAIILIIEIFISI
                     TTATIYFSLSPISSAGSEILSRTSPNIWDVIIAFTGGIAGIIGITRKKSGNILPGVAI
                     ATALMPPLCTSGYGLATKNIHIFLGAGYLFFINSFFIALSTLLVVKFMKIPTRNTLSE
                     VKQKKLKKMIIVSTILITIPSLMSAATMVNSFLNSTNLSNFIENEMPSEYILNKEINT
                     KNKTIELVVIGNTIDNSEEEKLQEALKYYNFSGYKLIIQQSTDNFPELKMYLDKIKEQ
                     DSGFIGDLEINKGKNNSSINNNVTSALNSVTSSLPGKFDDITKVYSGVLDNELPVFII
                     NHSKDYIDKDAINAYILSNPELKNAKIYFEKEDSNKENTKPQ"
BASE COUNT         7228 a         4356 c         2358 g         8804 t
ORIGIN      
        1 atataggctt aatttcttat ttaaaactat tttttactgc atctatagat tcttgaactt
       61 taactctagc atcatatgca gatttttcaa tatctttcat tgcactaata aactcatctt
      121 taggcattgc taatatttta tttctgttag catctaaatc acttttaaca gtgcttatag
      181 acatttttaa attatctatg ctttcaacta tctttctgtt ggcatcatta acttttccat
      241 ctgatggaga aactttgctg ttaagttcat tttttagatt tgtaagttca gtatctaggt
      301 ttttataata tttttcatac tcttcataag ttgcttgctt agtatcctct gttacctttg
      361 aaaaatcata ttctgtacct aaatttgcat caccaatata agtttttatt ttttcttcta
      421 gcattgtttt atattctgct ggagtagttg ctgatagtcc tgagttctct gtcttttcat
      481 tagtcttttt tgtacatcca accataccta taattccaaa gatcattact cctactaaaa
      541 gccaaattgt ctttttaaaa tttcttgtca tattaatacc tccacttaaa ataatatttt
      601 tcaaaattat cttccccata aaaacaaatt aatattattc aatttggtag gtattttttt
      661 gcataaaaaa acttttaagt tttttaacag caaatttaat attcgctgtt aaaataatta
      721 cttaaaagat tttaaaaact ctgctagaag ttgcctgttc taagctcttc agctattttt
      781 tcaatttttc ttaatctatg atttacccct gattttccaa ctggtggttc aagcatttca
      841 ccaagctcct ttaaggattc ctcaggataa gttaatctaa gttctgcaac ttctcttaaa
      901 ttcttaggaa gtcttgcaag acctatttct ctttctataa gctttatact ctcaacctgt
      961 ctaaccgctg catttacagt cttactaagg tttgcagttt cacagtttac aagtctattt
     1021 acattatttc tcatctcttt catgattcta atgttttcaa gttctaaaag ggcagtatga
     1081 gctcctatta tatttaataa atctactatt tgctctccct cttttatata aactatataa
     1141 ctattttttc tttgtataac cttagctttt aacccaaaag tattaattaa ctttcctaga
     1201 tcctctgcat attcttgaga gtgagtaaca aactctaaat ggtaagtttt ttctggatta
     1261 cttatacttc ctccaccaat gaaggctcct ctaatataag cttttttctt ttcttctgta
     1321 tcaactaaat tctcatctat atgatagtct agtgacatta ttccatctat ctctcttaat
     1381 atacctgtta tttcaagtaa tttttttact cccatttctt ctgttacaac aaccatataa
     1441 atattatttt tctttaaaga attacttttt ttaaccataa gctttgcatg tatgtcaaag
     1501 taatccttta atatagtaaa catcaatctt gcagatgcag gattttctgt tgtcattttg
     1561 aagcttattt gtctaccact aaaagcaagt gtaccacata ctttcatgat agctgatatt
     1621 tgagcaagag cttcctcttt tgatatatca atatatctac atatttctcc ctttactttt
     1681 gctgaaaatg acatggctac tccttcctat ttgaaccttc tcttttctta attctttgtg
     1741 ataaatatat atactctaaa attttctttc tatcatataa taaatgcttt tccattactg
     1801 tttcaatgag aagttgtgct aaataatccg aattatgtct tatataatcc cctttaacct
     1861 tagttaaatc tccatcaaca acacatagtc ccatcttttt aagcttatta atatcaagtt
     1921 ctacaagctc agaattttta tctaaatact tttcttttaa ttcttctgaa atatctttag
     1981 agtttgcaat tatacagtct accatatctt ttccacaatg atctaatata acttgaacat
     2041 ggtcagatac cttaaatcca tcagtctcac ctggttgagt cattatattg caattatata
     2101 ttttaaatgc tttacttttc tttatagctt tacttatatc ttttaccaaa agattaggta
     2161 atacactagt atataaactt cctggcccta aaactatagc atcagcatct tctatagcct
     2221 ctaaagccct atctaaagcc ttagcatcct ctggtattat ctttaatttt tttattggac
     2281 tactttggtc tattacttcc tctcctatta gagattcccc tcttacccta tgtccattca
     2341 taagttctgc ttctaaaacc atattttcta aagttacagg taaaacttca cccttaacgg
     2401 caagtacaga actcattttt tggacagcat cctcaaaatt ttcagatatt ccatccatag
     2461 ccgctaaaaa taaattacca aaactttggc cctttagtct accatcatta aatctatatt
     2521 gcaataattc gttcatcaaa ggctcagtat tagctaaggc taaaatacag tttcttatat
     2581 ctccaggtgg cagtattcct aagtcttctc ttaaaatacc agatccgccc ccatcatctc
     2641 caactgttac tatagccgtt acattagatg tatagtgttt taatcctcta agcatgatgg
     2701 aaagacctgt tcctccacct ataacaacaa tctttggccc tttaattaaa agtctatcat
     2761 cttctattag atcttctaaa tcatcttctt caatagataa atttatatat cctttattaa
     2821 ctaatgaaac cactgacttc acgcaccaaa ctattgattt ataccctact aacccagaaa
     2881 aaattattag tattataaat acagctttat catgaatggt ggttacatgc ctctcatcaa
     2941 ttaactttgc aatggcaaca gccattgtaa ttatagatgc taaagccaaa aaaatgtatc
     3001 ttttaacttt gataccaggc ttaagcattt ttttcatcat catagttttc tatctcccct
     3061 atttatatcc tcattaatat ctctatgctc taaggtaacg tgaaagttct tacataaaag
     3121 atctttatat aaagcgttag ctatggctac tgatctatgt cttcctcctg tacatccaat
     3181 agagataata agttgtctct ttccttcttt tatataatta gggattaaga attctgccat
     3241 atctgaaagt cttgataaaa atccttttgt ttcttcttga gcaaggacat aatttttaac
     3301 tggttcatct tcccctgaaa atggctttaa ttctggaata taaaatggat ttggtataaa
     3361 tctaacatca aaaactaaat ctgaatctga tggaatacca tacttaaatc caaaggataa
     3421 aacagttatt gaaagatttt gctcaactgg cttaccatct ccatataaaa gatttatttt
     3481 ttctcttaaa tttcttatag gataatttga agtatctatt atattatcag ctctatcctt
     3541 aagttctcta agtctcattc tttcttcact tataccagtt attattcttc catctggtga
     3601 aagcggatga cttcttcttg tttctttgaa tctcttaact aaaacctcat cagaagcctc
     3661 taagaataaa atttcgtaat taaaatcatt agcctttaaa tactctatgc tttcaaataa
     3721 atcatcaaag aatattccgc ctcttatatc tattactaat gctattttat ctattttacc
     3781 ttcactttga acacaagcct ctgcaaactt aggtattaac tttggaggta agttatctac
     3841 gcaaaagtat cctaaatctt ctaaacttct tgttgcttct gtttttccag cccccgaaag
     3901 tccagtaact attacaaatc tcatattatc atctcctata aatctataat ctgtttaata
     3961 acttcaaaac ataataaatg ctttaatcac ttagtaatta aagcaaacaa actaatttct
     4021 taaatagatt ataacataaa ataagtcaat tattacttat tttataaacc tctaatttta
     4081 tatatttttc tattttctac gtaaagaatt tttatattat gatacggcaa aaaagagtat
     4141 gcacatgcat actccttctt actaatctct accaattatt ctaacttcag tatgaagttc
     4201 aacatcaaaa ttttctttaa ctgttttttg aacatgagct atgacatcta aaacatcctt
     4261 agctgtagct ccacctttgt ttattacaaa acctgaatgc ttttctgaga cagcagctcc
     4321 tccaattgag aaacctttta atcctgaatc ttgaataagt tttccagcga aatatccttc
     4381 tggtctttta aatgtactac ctgctgatgg atattctaaa ggttgcttac tctctcttct
     4441 gtttgtaaga tcatcaatct tatcttttat actagcatac tctccgcttt ctagttcaat
     4501 tgtagcctct ataactacat atcctttttt cataactatt gaacttctgt aaccaagttc
     4561 taattcctct ttagttaaat ttttaatatt acaattttca tcaattactc tagctgattt
     4621 tattacatgt gccatttctc catcataagc tccagcattc ataaacactg cgcctcctat
     4681 gcttcctggt attccacacg caaattcaaa gcctcttaag ttattttcta aagcagcctt
     4741 tgatacatct tttaataaag ctccactctg agctgttaca caatttccct ctgttgtaat
     4801 cttgtttaat ttgttaaatt taattacaac tccagaaatg cccccatctt taactaaaat
     4861 attagaaccg ttacctaata taaaataagg tacattatat tccctacaaa gttttaaagt
     4921 attaactact tgttcttcat taacaggagt tactagtata tctgctggtc ctcctactct
     4981 aaaataaata tgttcgctca ttggagaatc cacagtaata tcttcttcat tataaaattc
     5041 acctaataat ttataaaact ccatgtattg attcataccc aaactcctca attttaaatt
     5101 taatatccta atttaatagt ataaattaat tcttgacata ttttcaatat ttagtacaaa
     5161 tttttttatt ttcctatgcc ataatattat atatattatc ctaagtcaat ttctgttaat
     5221 aaaaattaac ttgaaaaata ttgttttata ctctctgctg ctcttttgtc tatccctggt
     5281 gtatctaata attcttccat agatgccttt ttaatattat ctatacttcc aaacttcata
     5341 agaagatttc ttcttctctt ttccccaatt ctaggtatat cttctaatat ggaatttaag
     5401 gttcttttat ctcttaaact tctgtggtat gttatggcat atctatgaac ctcatcctga
     5461 actctagtta ttagattcat aaggcctgaa ccccttctaa taaggatttc ttcgttattg
     5521 aatataattc ctctagttct atgcttatcg tctttaacaa ggccacagac gggaatttct
     5581 ataccaaagt cctttagaac ctctaaggct atgtttactt gcccttttcc accatccatc
     5641 attatcaaat ctgggaaaat acaaaactta ccctttgagt attctaaatt tctctctttt
     5701 attttattta cttcctctaa tccatgagaa aatcttctgc ttaatatttc tctcatactt
     5761 tcataatcat taggaccttt aacacttttt atcttaaatc ttctataatc tgaattttta
     5821 gctttcccat tttcaaaaac taccatggtc cctactgagt ccacaccctg tatattggat
     5881 atatcataag cttctattct agcaggcaaa gaatctaatc ctaaaacatc tgcaagttca
     5941 gttaaggcag acttatttaa ctctttttcc tcaaccatct tttctttaaa ttgatcaagc
     6001 attattttag cattatttct aaccatatct aaaagattct ttttatcccc cttcttaggg
     6061 acttttatcc aaaccttaga tcctcttttt tctgtaagaa acttttctat aagctcttga
     6121 tcctctattt cctctggcac ataaatggtc ttaggtattt gtgcagttcc tccataaaag
     6181 gatgctataa aggaacttat cacctcttta actggatcat cactaatatt ttcaatcata
     6241 aaatgctctc tgcctgtaac ttttccctct ctaacaaaga aaacttgagc acatccatcc
     6301 ttttcatcag tatataagtc tataaaatct tcatcgcctt cttttacagt aaacattttt
     6361 tgcttttcgc taatcaattc tatggccaaa attctatctc taattttagc agccttttca
     6421 aactctaatt cttctgcagc cttttccatc tctaatttta aattttttat tatggatgta
     6481 tctgtaccat ttaaaatgtt tataatttca tctatcattt tccagtactc agccttagat
     6541 acataaccag cacaaggagc tttacaaaga ttaatatgat aatttagaca tgctcttgta
     6601 ggctctccac cttccacaat agccttttta caggttctta agggaaataa cttctttata
     6661 agtcccataa cctcataaac ggctgtacca ttagtgtatg gtccaaaata tctattccca
     6721 tccttagcaa aattccttgt aacataaacc cttggaaaat cctcattagt ggttattttt
     6781 ataaagggat aaaacttatc atcctttaag gctatgttat atcttgggct atatttttta
     6841 ataagattac attctaaaat taatgcttcc atttcagaat ctgtaactat gtactcaaat
     6901 tcagctatat tcttaaccat agctctaact ttttcagagt gattcttaga gttttgaaaa
     6961 tattgcctta ctctattttt caataccttt gcttttccta cataaataac ttctccaaga
     7021 gaatttttca ttatataaac tcctggttta tcaggcaata tctttaattg gtgttgaaaa
     7081 tcaaacatca ttatcacctc gcatctttta ttataaaact tatttttata aattaaataa
     7141 catttatgta aaatcactat aaattaatac gccctaacat actatttttc atagtaatat
     7201 tagggcgatt atttttttat taagtattta gttatattct ataattattt attaaaatat
     7261 gcttgcatta ccctagctgt aacaggggct gccacctttg aacctgagcc accattttca
     7321 actattgttg caacagctat tttaggatca tcagctggag caaaagctat taaccatgca
     7381 tgagaatttc catcaggaac ctgtgctgta ccagtcttag ctgccacatt cattccttta
     7441 aaatatcccc agttatcatt tattctatct tttgcaacgc cttgcatata ttcatttatg
     7501 atttgagctt gttctttact cataactgta tcttttttag ttgagctaaa ctcttttaca
     7561 gtatccccat ctttatccaa aactttacta actatataag gttccattct aaccccatta
     7621 ttagctactg ttgctgcaac cattgccatt tcaataggac ttgctaaaac ttctccttga
     7681 ccaatagcat cataagctat aagtccttta tcaatagttt ttctatctgg gaaatttcca
     7741 gaataagctt taagatttcc taaatataaa tctttattaa atccatactt ttctgctgtc
     7801 tctcttagtt ttttgtttcc aagctcttct ccaagaattc ctccaaatat tacgttactt
     7861 gaaactgata aagcttttct taagcttata tttccataag catttccatt ttcatttggt
     7921 aaatcttttg taccaacatt tatagaacca gtatcattaa aagttctttc aattacccct
     7981 ggaagatttt ctaaagcaga tgttaaagtt acaaccttaa atgtagatcc tggaggatat
     8041 tgaccattta aagctctatc aactaatatg gcatccttag gatttccatt agggttatta
     8101 gcaatttcat atgtttcttt catgttatta ggattaaatc ctggctttga aacactggct
     8161 aacaccgctc cagtcttagg atctaaggca actacagccc ctctattagc tcctaaggct
     8221 gcataggctg ccttctgtaa atcagtatct aaagttgtta caatactatt tcctactttt
     8281 ttatcacttt catttctttc tttaaaatct tttttcaagt taaatgattt taagaaagct
     8341 tttaatgata cattttcata agtagttaac tcttttgaat atgctccttc taatcctgca
     8401 tttccatata ttccactagc atatccaatt ggatgagaat atatttctcc ataaggatat
     8461 tctctttttt gagttaaatc accactttta gtacttttag ctagaacatt tccatctcta
     8521 tcttttattt ctcctcttat gacctcgttc tgagcagcca taacagcaga gttgctatcc
     8581 atctcagcta tctttggaac ttgtattaat tggaagtaag ctatataact tataagccct
     8641 actaatagca ctaaatagac aaacataact ttttttatgc ttcctgataa atcttttttc
     8701 tttgccatct atcttagtcc ctcctctgaa attttctgaa gtattcctag agctatgaac
     8761 actgtaagca tagatgttcc accataacta actaaaggta aagttatacc cgttaatgga
     8821 attactgaaa atactccgcc tataattact aaaacttgag caactataag tgtactaaaa
     8881 ccaacagcat ttaattgtga aaatggatca tctgtattta acgccgctct tattcctcta
     8941 tagaataata agaaatataa taatagtaat cctataccaa aaacaagacc taattcttgg
     9001 gctattaagg taaatatgaa atctgtagat gcaaaaggaa ctaatttagg ataaccatta
     9061 taaagtcctg tcccaaacat tccacctaga gacattgcat aaaatccttg aactatctga
     9121 taactttcat tattagcata tttccaaaca tctttccaaa tcattactct ttttttaaca
     9181 tgattaaata agaaataact cgctgttcca cctaatgaaa ataatactaa tccagtaccg
     9241 acatatttcc aattacaagt agctatgtaa agcatagtta tagatacaaa aaagaacata
     9301 agagctgatc ctaaatcttt ttgtaaaacc ataaatccta aagagtacat tacaatcaat
     9361 gcaggttcta aaagttgttt aaattcatat tttatattat ccttcttttc atatttcata
     9421 agggcggatg ctaaataaag tataaaaaat attttaccga tttcagaagg ttgaaaacca
     9481 aagctaccta tataaaccca gtttttagaa ccatttacag ttctaccaat aatcatagcc
     9541 attgccataa aaatcaaagt accacccata taaatatact tatatttagc aaaacttttt
     9601 aagtccggca taactattac aattcccata taaatagcta ttcctaaact aaaccaaatt
     9661 aattgtttaa ttgctaattt aggatctaat ctataaagaa ctgctattcc aactacagct
     9721 aaaacagctg caaaaataag catatattta tccccatcag gataaaactt tcttattatg
     9781 aaatgtgaga atccaataat aagcattaat ataccagcta ttataaaagc cattttgtct
     9841 ataggagttt gcttaatagc tatattagtg aataaggcca agcataaaag atagactaat
     9901 agcaacatcc ttttttcata ctttaaagta gtattcatat tatcacctct tttatcctat
     9961 aaccttaaat acagtgcttc ccattcttac ttgatcattt acttttaatc taactcttcc
    10021 ctctactttt gagtcgttta taaatgtacc atttgtactt gctaaatctt ctaaataaaa
    10081 atcattattt ttaacataaa ttttagcatg atatgatgat acaaactgat catttagcac
    10141 aattgaatta tcttctcttc taccaataga tatagtactc gtcactggta taaccgagcc
    10201 aatacctaga gttccatcag agattgtctt taagacttct aatccatgtg ctttctttcc
    10261 aggggctttc ttctttttcc cgcctttaac atctttatac attatcttta atgcataata
    10321 aattatggaa tatagtatta ttataaatat tattccaaaa acccatgtca ttagtttcga
    10381 aaacatcact tcacctctac atattttata tttcttttat attttacttt atttaataac
    10441 ttgattatac aataaaagat tacaatttca taattataat taaaatccct aattattata
    10501 tcatctaaaa aagtgtaatt tatgatattt atatttttat ttctcttctt tttatatttt
    10561 ataacattat attaatttta taactatcaa attactaaat ttatctaata tatatgaata
    10621 aaatatagct atcagaattt acaaccataa attctggtag ctatttaaat tgatctatta
    10681 ttaaatacta tatttacttt ttaaaattat attcctttat taaagatatt tctttaaata
    10741 tttacctgta taggattcct tagtctcagc tattttctca ggagttcctg tggcaataat
    10801 agttccacct ttatctccac cttcaggccc taaatcaact atataatctg cacatttaat
    10861 catatctaaa ttatgctcta ttactattac tgtatttcct ccatcaacta atctttgaag
    10921 tatttttaca agtctattta catcgtgtat atgaaggcct gttgtaggtt catctaagat
    10981 atataaggtt tttcctgtac ttctcttaga taattcatag gctaatttaa ttctttgagc
    11041 ttctccacct gataattgag ttgaaggttg acctaatctt atatatccta aaccaacgtc
    11101 cattaaggtt tgaagcttat tttttattct tggaatattt tcaaagaact ccaaagcttc
    11161 ctcaacagtc atgtttaata catcggctat attttttccc ttatatttaa cttctaaggt
    11221 ctctctatta tatctttttc ccttacaaac ttcacatgga acataaacat cagataaaaa
    11281 ctgcatttct atctttatta ttccatctcc actacaagct tcacatcttc caccttttac
    11341 attgaaagaa aatcttcctg gtttataccc tctcatttta gcttcttgag tttgtgagaa
    11401 aagctctctt attatatcaa aggttccagt atatgtagcc gggttacttc ttggagttct
    11461 tcctatagga ctttgatcta tatcaataat cttatcaata ttttcatagc ctgttatttc
    11521 tttaaacttt cctggtaaat ccttagcttt atttacgatt ttatttaatc ctttataaag
    11581 aatttcatta actaaggtac tctttccaga tcctgagact ccagtaacca tagttaaggt
    11641 tcctaaagga aatttagtag taacattttt taaattattt tccttagcac ccttaactgt
    11701 tatgaaattt ccattgcctt ttcttctttt ctctggaagc tcaacctttt tagccccagt
    11761 taaatactta ccagttaagg aattttcatt cctcattatt tcatctaaag ttccttttgc
    11821 aactatctct ccaccatgct ctccagctcc tggccctata tcaactatgt aatctgcttc
    11881 tctcatagta tcttcatcat gctctactac tataagagta tttcccacat ctctaagttg
    11941 ttttaatgtg gatataagtc tatcattatc tctttgatgc agacctattg aaggctcatc
    12001 taatatatat aatactccca taagttgaga tcctatttga gtagccagtc ttattctctg
    12061 agcttctcct ccagataaag ttcctgcttt tctagctaaa tctaagtaat ctaagccaac
    12121 atttattaag aaacttaatc ttgattgaat ttcttttata atttgactac ttattatctt
    12181 atccttttct gagaaatcta tggagtttat aaaatctaac tcttctctta tagccatgct
    12241 tgtgaattca aatatatttt ttcctcccac tgtaactgct aaggcttctg gcttaagcct
    12301 tgcaccctta catttaggac aaggattatc actcatatat ttttctattt cagccttcat
    12361 ggtatctgag ttagtttcca tatatcttct tcttaaattg tttatttccc cctcaaagga
    12421 atgattatat acagccgtta cattttcttt tgtatatgta acctttaatt tttctggctc
    12481 tccatacatt aaaacctctt gaaccttctt aggtaaatcc ttatatggag tgtctaaatc
    12541 aaagttgtac ttttccatta aagctttaag aacacaataa gtccaagatt cttccttcat
    12601 tcttgagtct ccccaagtag aaatagctcc tcctcttatg cttaaatcct tattaggaac
    12661 aactaaatcc tcatcaatct ccattaaagt tcctaatcca tcacatcttt cacattttcc
    12721 aaagggtgag ttaaatgaaa acattcttgg tgcaagttca tctatactta taccacaatc
    12781 agcacaagca aaatgctcac tataaagtct atcttcctct cctattatat taactaaaac
    12841 taatccttca gccattttaa gagatgtttc tatagaatct gttaatcttc cttctattcc
    12901 gtccttaaca attattctgt caactacagc ttctatatta tgctttatat ttttttcaag
    12961 ttttatctga tcttctgtta aatcataaat ttctccatct attctagctc taacaaaacc
    13021 ttgcttctta atattttcaa gaactttttc atgagttcct tttcttcctc ttattattgg
    13081 agccaagatc attattttac ttctctcttt aagttccata atttgatcta ctatttgatc
    13141 cacagattgc tgagtgattt cttttccaca cttaggacaa tgtggtactc ccactcttgc
    13201 atataataat cttaaataat catatatttc cgttactgta cctacagtag atcttggatt
    13261 cctagaagtt gtcttttggt caatagatat agcaggtgaa agtccttcta tggactcaac
    13321 atctggttta tccatctgtc ctaagaattg tcttgcataa gatgataaag attctacata
    13381 tcttctttgt ccttctgcat atattgtatc gaaagctaga gaacttttac ctgaacctga
    13441 aagcccagta aaaacaataa gcttatccct aggaatttct aaacttacat tttttaaatt
    13501 atgtactttt gcacctttta ctattatctt atccttcata aaactctgct actttaactc
    13561 tatctcaaaa tatccatatt aagaaattac cttctcaata taataactta tcatatgaat
    13621 ctctctttca taaaaagtta tctttttact acttaaaaaa ttatatttta gcttcttact
    13681 cttaaatatt ttgagtagta gcttctccct tcttttatat ttttaaaatt tatatttcct
    13741 ttaaaatctg tctatcccac ttaatatctc tctataattt gtgcttaata tatagatata
    13801 aatctttggt ttataataat aagattacga aaatctaaat aacttcatat attattatgt
    13861 ctataaaatc tatgtattat ctaagaaagt acactaagat ttctctaaag ttatttaagt
    13921 aaattcaaca tattatctta tcaaaccaaa cataagttcg cataacaata ttttaattct
    13981 ctctcattta tatgtcaaat tcttttttta taataattat tatccttaat ctttttaaat
    14041 tttcaaataa ttgattaaat catgtttata ataaatttag taactataaa tcttattttt
    14101 tcatatgata catattcttc tagattttcc ttaatagttt ttaacttgct atacccaacc
    14161 ttatctatgg catctaaaac caactcttct tccctttttg aaaataaaga atcaaaatct
    14221 acttcaaagt ctattttatt tccctcttct aaatattgct gaatatgaga aagcactgta
    14281 gtaatagaaa gttctctttc ctttacaata gtatggaatt ttttcccttc tctaagcata
    14341 tctatagtaa tttcaaagga ctttttcttc ccatccttac cattaccttt tttaaacttt
    14401 tcttctagct catccttatt aattttcaat ccagattccc acttaatctc aatattgttc
    14461 accttaacat attcatttat tacatttaag aacatttctc catacttaag tattttcttt
    14521 tccccaacac cacttatttc tgaaagctgg ctctcattta atggtattct tacactcatc
    14581 tctttaagtg tattatcacc aaatactata tatggaggta ccccttcttc tgaagcaatt
    14641 tctcttctta aacttcttaa taaatcaaat aattcattat ttttagatac cctcttagct
    14701 tttatttttt ccttaaacat aactttttca ttacccttaa gaatatctat ggaactttga
    14761 ttaagtctaa ctacagggta ctcccctata taatctatat atctatgcga aattagagtg
    14821 tttataaagt ccttaagccc ttctttagaa taatccttca ttattccata agtacttagc
    14881 tgatctaacc ctaattcaat tatcttttta ttttttgagg ctcttaaaac atctattatt
    14941 actcctattc caaagggtct tttcatccta taaacacagg aaataacttt ttgagcatct
    15001 attgttttat caactaattc cccttccatt tcacaattgc tacaattatg acagtcttcc
    15061 tctaagtctt ctccaaaata attaagtata aatcttctat aacatccatt agaataaact
    15121 aaatccatca ttgtttgtaa cttacttaat tcatttatct ttctttcagg atttaaggtt
    15181 ccagtttcta ttatatattt ctgtgtctga acatctcctg gtgaaaaaat aagaatacac
    15241 tctgatttct ctccatctct tcccgctctt cctatttcct gataatatcc ctctatgttt
    15301 ttaggcatat tattgtgaat tacatatctt atatttggct tatctatacc cattccaaag
    15361 gcattagtag caaccattac acttacctta tcaaaaacaa aatcctcttg atttttcttt
    15421 ctttcctcat ctgaaagtcc agcatgatat tttgtgcatc ctattcctct tgaatttata
    15481 agctcacata aggaatctac ctctcttctg gtagaacaat aaataattcc agaaacatcc
    15541 ttattttctt ttaaataatc taatatatag tgagccttgt taactccctt ttctattatt
    15601 atttttaaat tatccctatc aaatccagaa acaaaaacct ttggattatt aagctcaagt
    15661 aattttacaa tatcttttct aacttcctct gttgcagttg ctgtaaaggc tgaaactaca
    15721 ggtctttttc ttaaaatatt tataaacctt ccaataaact tataactact tctaaaatca
    15781 tgtccccatt gagaaacaca atgtgcctca tcaacagcca cctgtgaaat atctatatta
    15841 gaaactaaat ttaaaaattc catagattca agtctttcag gtgccacata taatattttt
    15901 atatgtccat ctttaacact ttgaaatata ttatttatct cttcattact aagagtagaa
    15961 tttatatatg ctgactctat tcccatgcta tttatggcat caacttggtc tttcataaga
    16021 gaaataagtg gtgaaattac aactgtaacc ccctctaaaa tcaatgcagg aatttgataa
    16081 caaattgatt ttcctccacc agttggcatt atagctacaa catcatttcc acttataatc
    16141 tcatttatta tttcctcttg tccttttcta aactcactat atccataaaa actttttaaa
    16201 atttctttcg ctctattcaa tatttctctc cttccaattt ataaccaaaa aaatcattta
    16261 ttaaatacta attattttat cctctaaata aaaaggcaaa ctcaaaaagc ttgccttttt
    16321 tctatataat ctattatata ctatttttct aatttatatt gattataata ttcagataac
    16381 atttctaaaa tttgttttgc tattttctta tatgcctcat ctggcaaatt agcttcagat
    16441 acacgtaata ttcctggatc agctccaaaa cctacccctt ccattaaaac tactccattt
    16501 atctccgcta aacgtaataa aaaatcaata ggtttaaaat tattctttaa atattcttta
    16561 aaattaacat catataaatc tctagctaaa tcataaatgt ttattaaaga gtaatactta
    16621 gcattttctc tactatcatc ttctttaata cctaatgtat tacataagtc atgatatcta
    16681 tctctcacta attttttaga actttctata tacggatctc ttcccttagt aatcaacgaa
    16741 gttaaagcaa ataaagccat ttgaatttgt tgaggtgttg ataatccaga agtgtgataa
    16801 aggccaactg aacggctatc tgcaacaatc ctctcaataa atttaaactt tcttggttca
    16861 aaagttacaa ggccatatct tttgtctaaa tcctgtaatt tttcctctgt taatttactt
    16921 attaactcat caaaaacatt gttttcatta gcagctatta atcctaatct ccaccctgtt
    16981 acaccatata atttagagaa ggaataaact aataaggtat tatgaggtgc tacggaatat
    17041 atagtcttaa agttttcaac aaaggttcca taaacatcat cagttattat cattaaatcc
    17101 ttacgttctt taactatatc agctatgtga tttaaagcct cttcacttaa agccttagaa
    17161 gctggatttg aaggatttac aaaaaataaa gctttaatct ccttatttct taacttgtct
    17221 aattctgaat cctctataac ccaattattt tcttcctttg aaattaaatc tacttcaacc
    17281 atctcataat cttttaattc tggtatttgt aagtaaggtg taaatattgg agtgtttata
    17341 gcaatcttat ctccagcttt tattagtcca ttttctttta atgaattaaa tatatagcat
    17401 atagctgctg ttcccccttc tgttgggaat acattagttt cattagctaa ttttactcct
    17461 ccaaaaagtt ctttttctaa atacaatttt aaaatctttt ctgtatttac taaacatcta
    17521 tttggaactg gataatcatt tccaattgct ccatccacaa attctttaac taattcatct
    17581 ttattaacac ttaaagtttt aatgctataa tctatggcat cacgtaaaaa tttatctact
    17641 tcatgccccc catttaagaa atccattaat ctttcatata taccatctaa attggtgtat
    17701 ccagctaaat ttccctcatg tatagttctt cttgattcaa aaacaccaaa ttccactatt
    17761 ctacctaagg ctagtcttcc ttcagtattt atccagttag gatttcctct tcctgcattc
    17821 aaaaatttat tgtgcttttc attgttttta gctaacttta acatcttgct actaatttca
    17881 aaagctccta atccttctag ctttttttca agatcttttg ttatcatgaa taaccactcc
    17941 ttatagtaaa ttatccccaa atggtttata cctttatttt aacactttgt taagttttat
    18001 ggttaaattc ccacattatt taagcataat tttcctataa agataaaagt caatcccctt
    18061 aataattact atattccact aagattttag aattattact ctaaacatcc ttctaaaaat
    18121 tatatagata ttctctttat actatttgca agttttataa aaagttgttt ttatatagaa
    18181 ccttatcctt tacttttggt atagccctat agtataaaac tattaataaa aaatattatt
    18241 ctccagctac agaaagttcc ttaataataa tacttggact accgtatcct gaagttactc
    18301 cactttcctc taggtcattt cctacctctt caatattatt tagtaaatca aagaagtttc
    18361 ctgcaatagt tatatgtttt attgcccctt ttaaaactcc attttctatt tttatcccct
    18421 cagcagctaa ggaaaaatct cctgttactg catttgcacc tgaatgaaga cctgctaagt
    18481 ctattatata aactccattt tccacataac ttaaaagctc ttcaaaggac ttctctcctt
    18541 cttttatata aagattagta ggacttgctt ttactgtaga tttaaatcct gatttaaagc
    18601 catttccggt tggattaacc tttgacttct tcgcagtttt taaactgtgt aaaaaccctt
    18661 taaaaactcc attttcaatt atatttttaa ctcttgtagg taccccctca tcatcaaagg
    18721 agcaagttga aattcctccc tttaagaaag gattatccac aagggtaact ttattagacg
    18781 caatcttctc tccaatttta tcctttaaaa gtgttttatt ttcttgagct atatgaccta
    18841 agaaattatc ttccatagtt cttattaagg aagccataac ctctccatct ataataactt
    18901 tgtatttacc tgattttatg gaagtagctc ctttttttct taaagtatta tttattgctt
    18961 tcttcattaa ttcatctata ttaacatcat ttaacttagt tgaaacccta gaaactccat
    19021 ctacaatcat attttctcca tctttagcta taggagaaat tgaagcttga acaatagttt
    19081 tttcttcttt taaatcaatt ccttctgaat taactattac tctctctaac ttagttgtat
    19141 taaatttagt gtattcactt tttattattt caaaatttat ctttgatggc tcagtactta
    19201 actttaaagc cttttctatt tgctcctttg gagttaattt actaatattt tcatcatagg
    19261 aatttacttc tacatacttg gctttatccc caaatataaa ctcttcatct tcactttcta
    19321 caagttcagc acattcaatt gcttttaaaa gtaagatttc tattgattca tcatctaatt
    19381 tctctgtata tgagtatccc attttcccct tatatatacc tctaaaagaa actcctatag
    19441 atgtacttaa tccatatctt tcaacttctc cattagatga agtaatattt aaagcttcac
    19501 ttttaccata atataattca tatttttcaa aattaagttc cttagctcta ttaaataatc
    19561 tttctttaaa atccttaaat tccataataa caaatacctc ctatcttccc ccaactgtga
    19621 tttctttaac tcttaaaagt ggctgaccta cgttagttgg gactgctcca cttaaggaac
    19681 cacaaactcc ttgcccataa tctaagtttt ttccaaccat atctatgttc attaagatat
    19741 cagcaccatt tcctattaaa ctagctcctc ttacagccct atcaatcact ccatttttaa
    19801 ctaaatatcc ctcaagaacg ttaaaattaa aatcgcctgt tataggattt actgatcctc
    19861 cacccatttg cttagcatat aatccatttt ctatagattt tatgattttt tcattatcgt
    19921 cttctccatt tcctataaaa gtatttgtca ttcttgaagt aggagcatat ttataagatt
    19981 ctcttcttcc acttcctgta gaaggcatac ccattcttct tccattaaac ttatcaatca
    20041 tatatgactt aagtattcca ttttctatta aaacttttct tctagttggt tctccctcat
    20101 catctatatt taatgaaccc catgcattag ctatagttcc atcatctatt gcagtaacct
    20161 ttttagaagc tatttgcatg ccaagtttat cacagaatat agaatttcct ttagcaacag
    20221 cagttgcctc aagggaatgg ccacaagctt catggaatat aaccccacca aaggaatttt
    20281 caatggctac agtcatctta cctgctgggc aataatctgc atgaagcatt gttactgctg
    20341 acttagaggc ttcttttcct aaattactta aatctatagt atcaaaaaac tcaaatccca
    20401 tagatgcccc tggagatctt gttccggctt gattatctac tccatttgaa gctatggaac
    20461 ttatggctat tcttgatctt attcttctat cttcagtaaa taatccttca ctattagcaa
    20521 ttaaaaccct ttgatcctca tccttcatag taactgtaac ttgagatatc tcatgattat
    20581 atccctttgc tcccttataa gcttccttca ttttctcaat tttcttagct gaacttatag
    20641 atgaaggaac atataaaata ggatgaatat tggtatttat tctttcaact aaatttaaat
    20701 taactgtatg tccatctata acgtccccta aagctaaagc cgccttttta gcagtattaa
    20761 ctaaactcat aagggaatca tcatttgtat atgcatatat gcactttagt cctttaaaaa
    20821 ttcttatacc aattccataa tcttttcctg aaataatatc cttaacagcc cctgaagtta
    20881 atacaatatt attatttaac ttttcttcaa caaaaacttc tgcaaagtct cctcctgtac
    20941 taagagctac agaaagtact tttttaatag tatctttact taacataaaa tcactccccc
    21001 aattaataaa caattatctt taataagatt atgcctagtt ttaaatattt aattctattt
    21061 taattgtata aacatgaaaa ttatataatt aaaagaaata ttatcattat aaaaaatttt
    21121 aaattaatta gctttttcaa tccatcttaa aggtatcatc tatttacata agatttgaaa
    21181 agtgacatac tatgctaatt agcttaaaat ataggttatt gaacttattt ttaaattaaa
    21241 ggaaaaaggg gaattaatat gagtattact aaggaaagat tctttaatga atttaagaaa
    21301 gatcaagcta catatgatga aattcatgaa aatatattag atggggttaa tataggtggg
    21361 gctaatttta tcatattaat gtgtgccata ataatagcct ctgttggttt aaatatgaat
    21421 gctactgctg ttattatagg tgcaatgctt atttctccac ttatgggacc aataatagcc
    21481 atcggatatt cagtaggaat ttataattta aaacttttaa aaaaatctgc aattatacta
    21541 ataatagaaa tattcataag tataactact gccactattt atttttcttt aagtccaata
    21601 tccagtgcag gaagtgagat tttatctaga acctccccta atatatggga tgtaataata
    21661 gcttttactg gaggaattgc aggaataata ggtataactc gtaaaaaatc aggaaatatt
    21721 ttacctggag ttgccatagc aactgcctta atgcctccac tttgtacaag cggttatggt
    21781 ttagccacta aaaacattca tatattctta ggtgcaggtt acttattttt tataaactca
    21841 tttttcatag cactttcaac cttacttgtt gttaaattta tgaaaatacc aactagaaat
    21901 actttatcag aagtaaaaca aaagaaacta aaaaaaatga tcatagtttc aactatttta
    21961 attactatac caagtttaat gtcagcagca actatggtta atagcttttt aaatagtact
    22021 aatcttagta actttattga aaatgaaatg ccttctgaat atattcttaa caaggaaata
    22081 aacacaaaga ataaaacaat agaattagtt gtaataggta acaccataga taattcagag
    22141 gaagaaaaat tacaagaggc attaaaatat tataatttca gtggatataa acttataatt
    22201 caacaatcaa ctgataattt ccctgaatta aaaatgtatt tagataaaat taaagaacag
    22261 gattctggtt ttataggtga tttagaaata aataaaggaa aaaataactc tagtatcaat
    22321 aataatgtta catcagcatt aaactcagtt acttcatcat taccaggaaa gtttgatgat
    22381 ataactaaag tttacagtgg agttttagat aatgaattgc ctgtgtttat tataaatcac
    22441 tctaaagatt acattgataa agatgctatt aatgcttata ttctatctaa ccctgaatta
    22501 aaaaatgcta agatttactt tgaaaaagaa gattcaaata aggaaaatac aaaacctcaa
    22561 tagcttttat attaaggaac ttctaaaagg taattttaaa aaacaattta aaaaatgatt
    22621 ctaaaagaaa ataggaaatt ttaaacttca taatatacaa cacaaacaaa gagctacaaa
    22681 agattttaaa ataatttatg agttctaaag gtttatactt ataaatttaa aataactttt
    22741 atagct
//
LOCUS       DAEVGI010000025        21743 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEVGI010000025 DAEVGI010000000
VERSION     DAEVGI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 21743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21743
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.025"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            202..984
                     /locus_tag="K8M77_000887"
     CDS             202..984
                     /locus_tag="K8M77_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HBI7085401.1"
                     /translation="MNRLLELSNEIFRIKTDDYTFPLFSSLHFKIILCVVITIFIAII
                     FKEKIKKWRYRRAFEIITALILISTQIFLAIWYSRFPNFYLKESLPLYPCRIAIIMAA
                     IGLIWKKNDFAKSIAYFWGTIGAIGALMIPITNSYIFPHITYYTFFIGHYFLLIASLY
                     LILIEEFKVKSENVKQALIFSFIFAICAFVANYLFDANYAFLNPPNGYEILNLINIES
                     NIITTSIFAYFIFVLGIFLMYLPFNISDYKKDSLFECSLIKY"
     gene            1242..2183
                     /locus_tag="K8M77_000888"
     CDS             1242..2183
                     /locus_tag="K8M77_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085402.1"
                     /translation="MLKDLNEKIKECRENMALKEILEKRNLSLENEFNSKEKELGELE
                     KSLNKELKDVQKLKSLSFSNLISNILRNKDEKLEKEEREYLETKLKYDNFKFKVEKLR
                     YDVKENNNRLGQLINIEGKYKDLISEKRELVKKFNLNIRDEIIEIENEIKELLSNKTE
                     ISEALREANNCLMISEETLKSLRSAKNWGIYDIVGGGMISSAIKHNRIDDAKTYMERL
                     SYSVDRLNKELGNVDTSIFNEGLNISGFSYTFDIFFDNIFSDFSVQVEINDSLYKIED
                     FRNNVLNLIRKLEEKENSIGLKVNLLNERLERLIEEN"
     gene            complement(2248..3603)
                     /locus_tag="K8M77_000889"
     CDS             complement(2248..3603)
                     /locus_tag="K8M77_000889"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7085403.1"
                     /translation="MNHDYILGKEKIFTALMKLSIPATVAMLINAIYNIVDTIFVGRE
                     IGALGIAGVSVYLPLQMIMMSLASLIGVGTASIFSRKIGNNDLEGANKAFGNLILLVY
                     LFSIFTFIFGFIFLEELVRFLGASNEVLPYAIDYARSILPGVLFFPICVAANNIIRAE
                     GSAKDAMCCMSIGMISNIFLDYLFICKLNMGITGAGLATSISKSLSFSYLTYYILNKS
                     SLKLKLKYLKFDFSILKKIISIGTSAFITQVSISLVALLLNHSLVKYGGNFALSIYGI
                     IYKLTLFIQMPLSGLVQGMQPIIGFSTGANNITRVINTFKLTLIISTFIATTLTLIIL
                     IKTDMFMKLFTTDINLINEGSKVLKSNIIMYPFLGIYLTSIGFYQSTGKGKESLILSL
                     LRQFIFFIPLSIILPSLFNLGIWGIWLAFPISDFLAFICTLVIAKITINKKAITPKII
                     N"
     gene            3832..6246
                     /locus_tag="K8M77_000890"
     CDS             3832..6246
                     /locus_tag="K8M77_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HBI7085404.1"
                     /translation="MRKIIHINDQWFYANDYKGEYLKNEFDFSNFERVDLPHTNIELP
                     YNYFDEKSYQFVSTYVKTLKFDNSVKGKKVFLDFEGVMIAAEVYLNGIHVGGHKGGYT
                     NFSIDITEALKINEDNILKVVVDSTERPDIPPHGYVVDYLTYGGIYREVSLRVVEPIF
                     INNLYARAYDCLKEEKRLELYIEINNFEKYRDDLEIVVDFGDDTFEETLSTKLPIEEG
                     TSIKNIEIDQLNMVKLWDIENPKLYEIKVKLLKGSEVIDEYKDNFGFREAEFRSDGFY
                     LNGRRVKLVGLNRHQAYPYVGYAMPQRVQEKDAEILKYELGLNIVRTSHYPQSVHFLR
                     KCDEIGLLVFEEIPGWQHIGDEAWQAESIKNVEEMIKKDYNRPSIVLWGVRINESQDS
                     HDFYVKTNAMAKSLDPIRQTGGVRYLENSDFLEDVYTMNDFIHSGGEKVLRTQSEVTG
                     QVDKVPYLVTEYNGHMYPTKSFDQECKKVEHAYRHLRVINESFGLDEISGAIGWCAFD
                     YNTHSSFGSGDKICYHGVSDMFRNPKYAAYSYASQKKVEDGVVLAPITLGAKGERDGG
                     AILPFTVLTNCDYIKIFKDGIYIDTYYPNKEKFPNLPHPPIEVSHILSMDSEIPLTEE
                     AKKEIKDFVLNKLKDSNLTNLAEEDFKYIEEFSERVNIPVFKIMSLVYKLAGGWGDKE
                     NSLIIKGFIDNKEVASKEIGELRSMNKLEVTPDDLELSLDKTSYDATRIVVKLLDNLG
                     EVLFLNNDFIEVEIDGPLSIMGPSKFGISGGAVAFWVRTQEKTGLCKIKVKSMYFEEE
                     ISIEVK"
     gene            7041..7994
                     /locus_tag="K8M77_000891"
     CDS             7041..7994
                     /locus_tag="K8M77_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBI7085405.1"
                     /translation="MSKILISILLSLGLCSGGAGVRYFNSNNISHKNVQANQSVSNES
                     EEKVEAQQGEESKEETNILSSNPFTKVFENISKNFSKDKVADTTKKEESVKNTPQDEE
                     AKVESNYENGKLEIIQSNNENKEVAQSNNEEANPSKDKQEEKPVENNKVEKETNKDSV
                     QKQENKPVENQVEKPVEQSNNSSSYIAQIEQMIFNKVNEERAKAGVAPLSYSNTMQKY
                     ARIKSEDMGVRKYFSHEDPNGQLITAKMKADGVSYNAWGENIAYLSGYSGDSNIANQF
                     MTNWMNSSGHRANILSPNFTSIGVGVYKIGNTYYATQEFMK"
     gene            8308..10119
                     /locus_tag="K8M77_000892"
     CDS             8308..10119
                     /locus_tag="K8M77_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085406.1"
                     /translation="MRGNKKKGTLLSNLKYIMVYAWSQDKVLFAQCGVYTILASISPF
                     LGIFLPKFLIDELLGQRRIEIILMTLIGFFLLSSVVNYSIAWLRCAYSPRVTKIRTDY
                     ITMISDKIMKMDFKNTEDPEVLNKIKSVMNAVMSNNTGVEGVYHTLLGLFGRLTAFVG
                     YISIVLFLSPWILLFLIINVLISYALTMRVKKYEYSQKEKAADKDRRTFYVFDTMYDF
                     AYGKDIRIYDLKNILIDKFKKFRGERIDISNDVQGKQLKVKIVDVILLVIRECVVYGY
                     LIYNVLFTGMGIGDFTMYFSTINGFGDWMKGILDDLAHIKAQNMYLDDMREFLEIKSE
                     DKEKTRDIPIDSSYEIEFKNVSFKYPKTDKYIYKNLSLKIKKGQRLAIVGINGAGKTT
                     FVKLLCRLYEPTSGEILINGVNIKDFSKEEYYKILSVVFQDIKTFAFTVAENVSLENL
                     EDVDREKVLHCIEKAGVGDKINSLQKGIDTSLLKILDGEGVELSGGENQKLALARALY
                     KNGKIVILDEPTSALDAVAEYNIYKGFDELIGDKTAIYISHRLASTKFCDVIAFFENG
                     EIVEYGTHEELLKKNGKYSDMFNIQAQYYKEESGALA"
     gene            10119..11909
                     /locus_tag="K8M77_000893"
     CDS             10119..11909
                     /locus_tag="K8M77_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085407.1"
                     /translation="MENKEKYTSDLYIIKHFLKLAHGISKTYIPTLFFSSIFAAAVPF
                     LNIIMPKYIIDELLGARRIKVLILLVLLTVLGNGVLNLINRILEKKCNIKNEKVVAGF
                     DLLIGKKVMDLDFENIEDPEILNLKERALFPIRNQGAIWQLTYNISKELTEILKIIGL
                     IGVILTLDWIIIALIILIVLLSSFMYKKSQRLQHKFFDSLIPLNREFRYYINTINDFS
                     AGKDIRLYNVAPLFVNKLDEFNINSTNVFIKMFSKLGVYEGLNEIVLQLQMVIVYGYI
                     TYKVLINSIGIGDFTMYTNAAINFSKSISNLFGSYVTIRQMCRYLQAFMKFENIESKH
                     TKEGKKIENLEKVNIEFKNVSFKYPRSKEFTLKNVSIKINEGEKLSVVGLNGAGKTTF
                     IKLLARLYDPTEGEILLNGVNINEYDYDEYMKLLSVVFQDFKLMSFSIKENIALNDFN
                     KVKDEEIEEVLIKSGFGKDLEKLPKGINTPVYKNFDEGGIEFSGGQAQKLAIARAVYK
                     DAPIVILDEPTAALDPKAEYEIYSKFNELIGKKTTIYISHRLSSCRFCDNIAVFQKGK
                     LIQYGTHDELVKDKGNEYETMYSAQAQYYV"
     gene            12130..12627
                     /locus_tag="K8M77_000894"
     CDS             12130..12627
                     /locus_tag="K8M77_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBI7085408.1"
                     /translation="MDKKLSNKAIKSWILGRTIGLIVLVIIYLGIIFVLPKMDIQWAD
                     YILNNHMKIVNILSFIIIGITVLSAYIEPFFEYKQWSYRIDEEEIFFTEGVFFKRSVT
                     IPIVRIQNINLSEGPVNRKFNLADVKIGTAEGSYKIPNLDKEEVEKIREFLRIKINEN
                     VREEL"
     gene            12636..14250
                     /locus_tag="K8M77_000895"
                     /pseudo
     CDS             12636..14250
                     /locus_tag="K8M77_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
     gene            14610..14894
                     /locus_tag="K8M77_000896"
     CDS             14610..14894
                     /locus_tag="K8M77_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI7085409.1"
                     /translation="MEDKFEKKSRVFKALGDSNRLKIIDLLSSGEKCACEILKFFDIS
                     QSTLSHHMKILSECGLVKCRKEGTWNHYSLNLNNANKSILFFMEIITCLD"
     gene            15013..15411
                     /locus_tag="K8M77_000897"
     CDS             15013..15411
                     /locus_tag="K8M77_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HBI7085410.1"
                     /translation="MKKVAFICVHNSCRSQMAEAFGKILGLNVFESYSAGTEERDKIN
                     QDAVRIMKEIGVDMELTQRPKLLREIPEVDIVITMGCNVSCPTLPCKHREDWGLEDPS
                     GKSNEEFIYTRDKIKEKVEDLVSRIKNGQI"
     gene            15428..16744
                     /locus_tag="K8M77_000898"
     CDS             15428..16744
                     /locus_tag="K8M77_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7085411.1"
                     /translation="MKKIDLTKGNVLSVLTTLALPIMGSSFLQFAYGLIDMFWVGGLG
                     SSAVASIGSSSFFLGLGYSINALVVTGTGIKVSHEVGRKNEIETEDYIRNGIIMNFLI
                     GLIYALVLVVFGKGFIGFLNLNDPLVERDSYIYLAVSAPVLFFSFFNYLFTRILASFG
                     NNKLALKISATGIILNMILDPILIYTFKLGVFGAAVATLLANILMFFMYIYYGKGIFK
                     IDLKKKINYEKVREIARLGFPMASQRVLFTLVNIILARIIASFGADAIAAQKIGLQIE
                     SVSFMVIGGLNGAIAGFTGQNYGAKKLNRVNEGYKKALLLGAIYSVLVSALFIFIPGL
                     LVGLFVKDEATIIIGSSYLRVLGIVQIFSSFEMISNGVFSGLGLPKIPSAISIIFTML
                     RIPMALVLVKFLGINGVWWSIAISNILKGSTSCIIYRLKIYRNYIV"
     gene            16933..17571
                     /locus_tag="K8M77_000899"
     CDS             16933..17571
                     /locus_tag="K8M77_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085412.1"
                     /translation="MKRILIIEDTDTIREELTKLLTRYGYEVLAPTDFENILEYVKEN
                     NSDLILLDINLPYFDGYHICREIRKDSNVPIIVTSRDSDMDEIMSMNLGADDFITKPY
                     NTQVLLARIASLLKRTYSLSEQEVLTHGDLKLNLSNGTISCRGKEEELTKNELKILSH
                     LLKNKGNVVSREDLMDYMWNSDIFVDDNTLSVNVTRLRKKLEEVGVKNSMIT"
     gene            17547..18326
                     /locus_tag="K8M77_000900"
                     /pseudo
     CDS             17547..18326
                     /locus_tag="K8M77_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475116.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            18561..19856
                     /locus_tag="K8M77_000901"
     CDS             18561..19856
                     /locus_tag="K8M77_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBI7085413.1"
                     /translation="MGILDLFKAKENNELKEEIKELKKLLNPEQQEIANLVKEIDKLK
                     LEKDKISKEVDKLFSKKQELSNKIKLKKKDLILLDDEILMQEFGLYKPIYDFESSEKY
                     KQAIDNLRVVQKEMIKNKSAVIYSNNWTVDGSKAKGRKMTNDNIKQIIMAFNIECDNL
                     IAKVKYNNIQSIQKRIEKTFERLNKLNESNQVRLTSKYLECKLSELKLVHEYQVKKQE
                     EKEEQKRIREELREEAKLKKELEEAKKNTLKDITHFENALSKLNEQLKSNNLSDEEIK
                     NLQLKKEELEKNIDNLNLSLKDIDYRQENQRAGYVYIISNIGAFGKDVYKIGMTRRLE
                     PMDRIDELGDASVPFNFDVHAMIFADDAPKLENALHKAFENKKLNMVNQRREFFNVTL
                     EEIEKVVKENFDKTVEFKKEPEAEQFRQSLKIKDTVLTY"
     gene            20118..21686
                     /gene="cloSI"
                     /locus_tag="K8M77_000902"
     CDS             20118..21686
                     /gene="cloSI"
                     /locus_tag="K8M77_000902"
                     /EC_number="3.4.22.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain"
                     /protein_id="HBI7085414.1"
                     /translation="MFKKKLSLLMATITIGSVLLGGASTVSAAPRQKHKTVSEKIKEA
                     EKTEGDKKLTVMVYADCDNNLEEYILNDIEEMKEGYKNNPNLNIVVLVDRIPGYSNDS
                     KVLGSNFEDTRLYKIGENSAERISGKSEFPEITTTSNYEANMGDANTLKKFIKFCKKN
                     YEADKYMLIMSNHGGGAKDDKDRASTVNKAICWDDSNNKDCLYTGEISDVLTKDESVD
                     VLVFDACLMGTSEVAYQYRPNNGSFEAKTLVASAPVVWGNGYPYDKIFSRLKSTKGDN
                     GEVDSTLGGKEKIFEPSLVTNNELGALFVEEQRDSVNSYGVTDQQLSCYDLSKIETVK
                     KSVDALARNLSKNNKKDAIENLRGTGKNAPTMHYFKNYDEYEWIEYPYFDLYDLCEKI
                     SLSNEFDETTKKLSKNVMKNVDQLILYSFAGNDFKGFKEGKNGISIFLPDGNRNYYDQ
                     YSGQVIPHWAIQRWYNPLDTNAYGLRSGYGKLAWCKDGLDPKINKVGNWFELLDSWFD
                     KDNTSLGGYNRYRY"
BASE COUNT         8750 a         2016 c         3796 g         7181 t
ORIGIN      
        1 atagcttaaa atcaaaagca cttatatatg tacggagggc taacttcgga atttacgcct
       61 ttggagagcc atacaaaatc gtagtagctt aggcaaggcg aggctttatg aagaaggaat
      121 aatctccata tggatatatt tgaccatatt ttgagtagca ggaaactact cagcataata
      181 tttttgagta gggagaatac tttgaataga ttgttagagc ttagcaatga aatatttagg
      241 ataaaaacag atgattatac tttcccgtta ttttcatctt tacattttaa gattatttta
      301 tgtgtagtaa taaccatatt tatagcaatt atatttaaag aaaaaataaa aaagtggaga
      361 tatagaagag catttgaaat aattacagca cttattttaa tatcaactca aattttttta
      421 gcaatatggt actctagatt tcctaatttt tatttgaagg aaagtttacc tttgtatcca
      481 tgtagaattg caataattat ggcagcaata ggtttaatat ggaagaagaa tgattttgca
      541 aaatcaatag cttatttttg gggaacaata ggagctatag gagcattaat gatacctatt
      601 actaattctt atatttttcc acatataact tactatacat tttttatagg acattatttt
      661 ttattaatag cttcattata tctaatatta atagaggaat ttaaagtgaa aagtgagaat
      721 gtaaaacagg cacttatttt ttcttttata tttgcaattt gtgcttttgt agcaaattat
      781 ttatttgatg caaattatgc ctttttaaat cctccaaatg gatatgaaat acttaacctt
      841 ataaatattg aaagtaatat tattacaaca tcaatttttg catattttat ttttgtatta
      901 ggaatattcc ttatgtattt gccatttaat attagtgatt ataaaaaaga tagcttattt
      961 gaatgttcct taattaaata ttagtcttac taaagaaatt tacacaaata atatgggttt
     1021 gattatttta atttaataga gcttaggaag aattataata ctaagctaag taaaatttag
     1081 gatataagta tattaggggg ttattttaaa aatgtattaa atatatgtag aagattttag
     1141 ggaggtgaaa agggtcatat taaaggtttt atataaaatt tatgaatata ttatctcaaa
     1201 attttttata agatttattt ttatgcaccc taaaagtagt tatgcttaaa gatttaaatg
     1261 aaaaaataaa agaatgtcga gaaaatatgg ccttaaagga aattcttgaa aagagaaatt
     1321 tatcacttga aaatgaattt aatagtaagg agaaggaatt aggagagctt gaaaagagtt
     1381 taaataagga gcttaaggat gttcaaaagt taaagagttt atctttttct aatttaatat
     1441 ctaatatatt aagaaataag gatgagaagt tagaaaaaga agaaagagaa tatttagaga
     1501 caaaacttaa atatgataat tttaagttta aggtggaaaa gttaagatat gatgtaaaag
     1561 aaaacaacaa tagattaggg cagttaatta atattgaagg taagtataaa gatcttataa
     1621 gtgaaaagag agagttagtt aaaaagttta atctaaatat aagagatgaa ataattgaaa
     1681 tagaaaatga aataaaggaa ttactaagca ataagacaga aatttcagaa gccttaagag
     1741 aagctaataa ttgcttaatg attagtgaag aaactttaaa atccttaagg agtgctaaga
     1801 attggggaat atatgatatt gttggaggag gaatgatttc ttcagcaatc aaacataata
     1861 gaatagatga tgctaaaact tatatggaga ggcttagtta ttcagtagat agattaaata
     1921 aggaattagg gaatgtagat acctctatat ttaatgaagg tttaaatata agtggatttt
     1981 cttatacctt tgatattttc tttgacaata tatttagtga tttttcagtt caagtagaaa
     2041 tcaatgattc tttatataag attgaggact ttcgtaataa tgttttaaat ttaataagaa
     2101 aattagagga aaaagaaaat agtatcggtt taaaggtaaa tctactaaat gagagattag
     2161 aaaggttaat agaagaaaat taattattat agatatttta aaagagatat ttgtgtggct
     2221 tagtcacata agtatctctt ttaagtttta atttataatt ttaggggtta tagctttttt
     2281 attaatggtt attttagcaa ttactaatgt acatatgaat gctaagaagt ctgaaatagg
     2341 aaatgccaac catatgcccc atataccaag attaaataat gatggtaata ttatcgaaag
     2401 aggaataaag aaaataaatt gtctaagtaa agataaaatt aatgattcct ttcctttgcc
     2461 tgttgattga tagaagccaa tagatgttag atatatacca agaaatggat acataattat
     2521 attgcttttt aatactttac ttccttcatt tattagattt atatctgttg taaataattt
     2581 catgaacata tcagttttta ttaatattat tagtgttaga gttgtagcaa taaaggttga
     2641 tattattaaa gttaatttaa aggtattaat aactctagtt atattattgg ctccagtact
     2701 aaatccaata ataggttgca taccttgtac tagtccagat aatggcattt gtatgaataa
     2761 ggtaagttta tatattattc cgtatataga taaggcaaag ttgccgccat atttaactaa
     2821 agaatgattt agtagaagtg caactaaact aatagatact tgagttataa aggcggatgt
     2881 tcctatagaa ataatttttt ttaatattga gaaatcaaat tttaaatact taagttttaa
     2941 ttttaaagat gacttattta aaatataata agttaaatat gaaaagctta atgattttga
     3001 aattgatgtt gctaaaccag ctccagttat gcccatgttt aatttgcata tgaatagata
     3061 atctaaaaat atatttgaga tcataccaat agacatacaa cacatagcat cctttgcact
     3121 tccttcggct cttattatat tattagctgc tacacaaatt gggaaaaata aaacaccagg
     3181 aagtattgat ctagcataat ctattgcata aggtaaaacc tcattactag caccaagaaa
     3241 tcttacaagt tcctcaagaa atataaatcc aaatataaat gtaaatatgg aaaataagta
     3301 aactaataga attaaattac caaaagcctt attagctcct tctaaatcat tattaccaat
     3361 ttttcttgaa aaaattgagg ctgtaccaac accaattaat gatgccaagg acatcataat
     3421 catttgtaaa ggaagatata ctgagacacc tgctattcct aatgctccaa tttcacgacc
     3481 tacaaaaatt gtatcaacaa tattgtatat agcattgata agcatagcta cggttgccgg
     3541 aatacttaat ttcattagag cagtgaaaat tttttcttta cctaaaatat agtcatgatt
     3601 catataatac tcctaagtta gctttaatat acaattatta tatttaattt aattgtaaaa
     3661 tgagattagt atacttgaat aaaataaatc aataaatgag ataaatttat ttaaaaattt
     3721 cattactttt aattgttaaa agaaagttca taagaaaaca tgtataatgt atatatggta
     3781 ataattaact taaggtttat ataattaatc gtaaaattag gaggagaatt tatgaggaaa
     3841 ataattcata tcaatgatca atggttttat gcaaatgatt acaaaggtga gtatttaaaa
     3901 aatgagtttg attttagtaa ttttgaaagg gtagatttac ctcatacaaa tatagagtta
     3961 ccatataatt attttgatga gaaatcatat caatttgttt ctacttatgt taaaacttta
     4021 aaatttgata atagtgttaa agggaaaaag gtatttttag attttgaagg agttatgata
     4081 gccgcagaag tatatttaaa tggaattcat gttggtggac ataagggtgg ttacactaat
     4141 ttttcaatag atattactga ggctttaaaa attaatgaag ataatatatt aaaagttgtt
     4201 gttgactcaa cagaaagacc ggatatacct cctcatggat atgttgttga ttatttaacc
     4261 tatggaggaa tatatagaga agtttcctta agagtagttg aacctatatt tataaataat
     4321 ttatatgcaa gggcatatga ttgtttaaag gaagaaaaaa gattagaact ttatatagaa
     4381 ataaataatt ttgaaaaata tagagatgat ttagaaattg ttgtagactt tggtgatgat
     4441 acttttgaag aaactttaag tacaaagtta ccaattgaag aaggaacaag tattaaaaat
     4501 atagaaatag accaattaaa tatggttaag ttatgggata tagaaaatcc taagctttat
     4561 gaaataaaag ttaagttatt aaaaggttca gaagttatag atgaatataa agataatttt
     4621 ggatttagag aggctgaatt tagatcagat ggtttctatt taaatggaag aagagttaaa
     4681 cttgttggat taaatcgtca tcaggcttat ccatatgtag gatatgctat gcctcaaaga
     4741 gttcaagaaa aggatgctga gattttaaaa tatgaattag gactcaacat agttagaaca
     4801 tctcactatc cgcaatcagt acacttctta agaaaatgtg atgagattgg actattagtt
     4861 tttgaagaga tacctggttg gcaacatata ggtgatgaag cttggcaagc agaatctatt
     4921 aaaaatgtag aggaaatgat aaaaaaagat tacaatagac cttccatagt tttatggggc
     4981 gttagaataa atgagtctca agatagtcat gatttttatg tgaaaactaa tgctatggca
     5041 aagagtttag atcctattag acaaactggt ggggttagat acttagaaaa tagtgatttc
     5101 ctagaagatg tttataccat gaatgatttt atacacagtg ggggagaaaa agtattaaga
     5161 actcaaagtg aagtaacagg acaagtagat aaagttcctt atttagtaac tgagtataat
     5221 gggcatatgt atccaacaaa aagctttgat caagaatgta aaaaagttga acatgcttat
     5281 agacatttga gagttattaa tgaatccttt ggcttagatg aaataagtgg agccatagga
     5341 tggtgtgctt ttgattataa tacacatagt tcctttggtt caggagataa aatttgttac
     5401 catggagttt ctgatatgtt cagaaatcct aagtatgcag cttattccta tgctagccaa
     5461 aagaaagtag aagatggtgt ggttttagcg cctattactt taggggctaa aggagaaagg
     5521 gatggaggag caatacttcc atttacagtg cttacaaact gtgattatat aaaaatattt
     5581 aaagatggaa tatatataga tacttattat cctaataaag aaaagttccc taatttacca
     5641 catccaccaa tagaggtttc acatatttta tctatggatt cagaaatacc tcttactgaa
     5701 gaagcaaaaa aagaaattaa agactttgta ttaaataaat taaaagattc taatttaact
     5761 aatttagctg aagaagattt taaatatatt gaagagttta gtgaaagagt aaatatacct
     5821 gtatttaaaa taatgtcttt agtttataaa ttagctggag gttggggaga taaggaaaac
     5881 tccttaataa taaaaggctt tatagataat aaggaggttg cttcaaaaga aataggtgaa
     5941 cttagaagca tgaataagtt agaagttaca ccagatgatt tagaactttc attagataaa
     6001 acaagttatg atgctactag aattgtggtt aaacttttag ataacttagg agaggttctt
     6061 ttcttaaata atgattttat tgaagtagaa atagatggac ctttaagtat aatgggacca
     6121 agtaagtttg gaatttctgg tggagcagtc gctttctggg taagaactca agaaaaaact
     6181 gggctttgca aaataaaggt taagagcatg tactttgaag aagaaatttc tatagaagtt
     6241 aagtagagtt aacttattag tatttttata ggaagaatta agtaatttta aattaaagaa
     6301 atagattata ttttaaagtg aagttttgct ttagattaat ttaggattaa agatatattt
     6361 gaaatttaga atttagttaa ggaatttaaa ataaaaagag ggagttatat caaaacttag
     6421 atataagaat tataaatata tttggagagt tagtgaatta tactaagaat ttataatttt
     6481 tatttataag tttaaattga tataactcct ttttatgtaa cttttgaaag gacgacaact
     6541 gtcgaaatta taatttttaa gagtaagagg gtccattaat attattagct aaattaagaa
     6601 taaatatcct tgaaatttat atagaaatta ataggaaaaa atagggggga ggtgaataat
     6661 ttattattgt agagttattt ttactgtata accagtaata atattgggga attttaggag
     6721 aaaaactgga gatcgattaa atattcatat ataatttatt taaagtctat agacaagact
     6781 ttataattta ttcattccta agttagaaaa aagtgttgat attaaaggtt acaagaagat
     6841 gtaaaagcca agatgactta tacatataga ggtagttaag atagaacact ttttttattt
     6901 aatagctaaa tgatttttta gttgtattct tacttaaata taataaatta tttaagtagt
     6961 aatagcctct acaaagaaat actaaaagga ttttagttat acttattagg aaaaacaaag
     7021 gataactagg agggaaaaac atgagtaaaa ttttaatttc tatattatta tctttaggat
     7081 tatgttctgg aggtgctggg gtgagatatt ttaatagtaa taatatatct cataaaaatg
     7141 tacaagctaa ccaaagtgtt tcaaatgaaa gtgaagaaaa ggtagaagca caacaaggag
     7201 aggaatccaa ggaagaaact aatattttaa gcagtaatcc ttttactaaa gtttttgaga
     7261 atataagtaa gaatttttct aaggataaag tagcagatac tactaaaaaa gaagagagtg
     7321 ttaaaaatac tccacaagat gaagaggcaa aggtagaatc taattatgaa aatggaaagt
     7381 tagagattat tcaatcaaat aatgaaaata aagaagttgc acaaagtaac aatgaagagg
     7441 caaatccttc aaaggataaa caagaagaaa aaccagttga aaataataaa gtagagaaag
     7501 aaactaataa ggattctgtg caaaaacaag aaaataaacc tgtagaaaat caagttgaga
     7561 aaccagtaga acaatcaaat aatagtagtt cttatatagc tcaaatcgaa caaatgatat
     7621 tcaataaggt taatgaagaa agagctaagg caggggttgc acctttaagt tatagtaata
     7681 ctatgcaaaa atatgctcgt attaaatcag aggatatggg agtgagaaaa tatttttcac
     7741 atgaagatcc aaatggacaa ttaataacag ctaaaatgaa agctgatgga gtttcatata
     7801 atgcatgggg agagaatata gcttatttaa gtggatattc aggagattca aacatagcta
     7861 accaatttat gactaattgg atgaactcat caggacatag agctaatata ctttctccta
     7921 actttacttc tattggggta ggagtttata aaataggtaa tacatattac gctacacaag
     7981 agtttatgaa atagaataag aattttaaaa ggcgtgtatc aaaagtcatt ttgatatacg
     8041 tcttttttat aaagattttt agcttaataa attaattttt tataaaaagt aatatgaatt
     8101 tttatgggat ttttggtata ttgtgtataa tgtaagtggg ttttattttt tagtaataat
     8161 atttcttaca gttaacctat atagaatagc tgtggtatta aaaagtaaat tagagaaggt
     8221 aaaggagagg gggaaaaata tagtttaata aattagagct aaagatgaga aataagaggt
     8281 tttaattgga attaggggat ggaatttatg aggggtaata agaaaaaggg tactctttta
     8341 agtaatctca agtatattat ggtatatgct tggagtcagg acaaggtttt gtttgcgcaa
     8401 tgtggtgttt atactatttt ggcatctatt tcaccttttc taggaatttt tttaccaaaa
     8461 tttttgattg atgaactttt aggacagaga agaatagaaa taatcttaat gactttaatt
     8521 ggattttttc ttttatcttc agttgtgaac tattctatag cttggcttag atgtgcatat
     8581 agtcctaggg ttacaaagat tagaactgat tatataacaa tgataagtga taaaataatg
     8641 aaaatggatt ttaaaaatac tgaggatcct gaggttttaa ataaaataaa aagtgttatg
     8701 aatgcagtta tgagtaataa tacaggggtt gaaggagtat atcatacact tttaggactc
     8761 tttggaaggt taactgcctt tgtaggatat atatctatag tattattttt aagtccatgg
     8821 attttattat tcttaataat aaatgtctta ataagctatg ctttaactat gagggttaaa
     8881 aagtatgagt atagtcaaaa agaaaaggca gctgataagg atagaagaac tttctatgta
     8941 tttgatacaa tgtatgattt tgcttatggt aaagatataa gaatatatga cctaaaaaat
     9001 atacttattg ataaatttaa aaaatttagg ggagagagaa tagatatatc taatgatgtc
     9061 caaggaaaac aattaaaggt taagattgtt gatgtaatac ttcttgtaat aagagaatgt
     9121 gtggtttatg ggtatttaat atataatgtg ctttttactg gaatgggcat aggagatttt
     9181 acaatgtatt tttcaacaat aaatggcttt ggggattgga tgaaaggtat attagatgac
     9241 ttagcacata ttaaggctca aaatatgtat ttagatgata tgagagagtt tttagaaatt
     9301 aagagtgaag ataaagaaaa aactagggat atacctattg atagttctta tgaaatagaa
     9361 tttaaaaatg tatcatttaa atatccaaaa acagataaat atatttataa aaatctttct
     9421 ttaaaaatta aaaaggggca aagacttgct attgttggaa ttaatggagc aggtaaaaca
     9481 acctttgtta aacttttatg tagactttat gagccaacaa gcggagagat tttaataaat
     9541 ggggtaaata taaaagactt ttctaaggaa gaatattata aaatcttatc agtagtgttc
     9601 caagatatta aaacctttgc atttactgtg gcagaaaatg tttctctaga gaatttagaa
     9661 gatgttgata gagaaaaagt tttacactgc atagaaaagg ctggagtagg agataaaata
     9721 aattctcttc aaaagggaat agatacctct cttttaaaaa ttttagatgg agaaggggtt
     9781 gaattatcag gaggagaaaa tcaaaagtta gccctagcta gagccttata taaaaatgga
     9841 aaaatagtaa tcttagatga accaacctca gctttagatg ctgtagcaga atataatatt
     9901 tataagggat ttgatgaact aattggagat aaaacagcaa tttatatatc tcatagactt
     9961 gcaagtacaa agttctgtga tgttatagcc ttctttgaaa atggtgagat tgttgagtat
    10021 ggaactcatg aagaattatt aaagaaaaat ggcaagtata gtgatatgtt taatatacaa
    10081 gcacaatatt ataaagaaga aagtggggct ttagcataat ggagaataaa gaaaagtaca
    10141 caagtgattt atatataata aagcatttct taaaattagc tcatggaata tcaaagacct
    10201 atataccaac cttattcttt tcatctatat ttgcagcagc agttcctttc ttaaatataa
    10261 taatgccaaa gtatattata gatgaacttt taggagcgag aagaataaag gttcttattt
    10321 tattagtttt attaactgta ctaggtaatg gagtattaaa cttaataaat agaattttag
    10381 agaaaaaatg taatattaaa aatgaaaagg tagtggcagg atttgatttg cttataggaa
    10441 aaaaagttat ggacttggat tttgaaaata ttgaagatcc agagatttta aacttaaagg
    10501 aaagagcctt atttcccata agaaatcagg gagctatttg gcaacttacc tacaatatat
    10561 ctaaggaatt aactgagata cttaaaataa ttggattaat aggagttatt ttaactcttg
    10621 attggattat tatagcccta ataattttaa ttgttctttt aagttcattt atgtataaga
    10681 aatctcaaag attacagcat aaattttttg attccttaat tccactaaat agagagtttc
    10741 ggtattatat taatacaata aatgattttt cagcaggtaa agatataaga ctttataatg
    10801 tagccccttt atttgtaaat aaattagatg agtttaatat aaatagtacc aatgtattta
    10861 ttaaaatgtt tagcaaatta ggtgtgtatg agggattaaa tgaaatagta ttacaattac
    10921 aaatggttat agtttatgga tatataactt ataaagtttt aataaatagt ataggaattg
    10981 gtgactttac aatgtacaca aatgctgcca taaattttag caaatccata tctaatcttt
    11041 ttggaagtta tgtaactata agacaaatgt gtagatatct tcaggctttt atgaaatttg
    11101 aaaatataga atctaaacac actaaagaag gtaagaagat tgaaaactta gaaaaggtga
    11161 atattgaatt taaaaatgta tcctttaaat atccaagaag taaagaattc accttaaaaa
    11221 atgtttcaat aaaaataaat gagggtgaaa aactatcagt agttggatta aatggtgctg
    11281 gaaaaaccac ttttataaag cttttagcaa ggctttatga tccaacagag ggtgaaattc
    11341 tattaaatgg agttaatata aatgaatatg attatgatga atatatgaaa cttctatcag
    11401 tagtatttca agattttaaa ctaatgtcct tttcaattaa ggaaaatata gctttaaatg
    11461 attttaataa ggttaaggat gaggaaatag aagaggttct aataaagagt ggctttggaa
    11521 aagacttaga aaagttacca aagggaataa atactcctgt atataaaaac tttgatgaag
    11581 gtggaataga gttttctggg ggacaagcac agaaattagc catagctagg gcagtttata
    11641 aggatgcacc aatagtaatt cttgatgagc caacagcagc tttagatcca aaagctgagt
    11701 atgaaatata tagtaagttt aatgaactta taggaaaaaa gacaacaata tatatttctc
    11761 acagattatc tagttgtaga ttttgtgaca acatagcagt atttcaaaaa ggaaaactta
    11821 ttcaatatgg aactcatgat gaacttgtaa aagataaagg taatgagtat gaaactatgt
    11881 atagtgctca agcacaatat tatgtttaat taatatttat atttagaaaa taaaaagata
    11941 gggtttaatc acttttttag gtatatagaa gaaaaaagct tctatataaa tacttaggaa
    12001 agtgattttt tatattaaaa tagattcttt cttaaacttt gcttaatttt taggttttaa
    12061 attgacttaa taagactaat tatgatattt taaggaaaag ttaacagaaa ttgaatgggg
    12121 gatggactaa tggacaaaaa actaagcaat aaagctatta agtcatggat tttagggaga
    12181 acaataggat taattgtatt agttattatt tatttaggaa ttatatttgt tttacctaag
    12241 atggatattc aatgggcaga ttatattttg aataatcaca tgaaaatagt gaatatttta
    12301 agctttataa ttatagggat aacagtttta agtgcttata ttgagccttt ttttgaatac
    12361 aaacagtgga gttacagaat agatgaggaa gagatattct tcactgaggg agtttttttc
    12421 aaaagaagtg taactattcc tatagttaga attcagaata taaacttaag tgaagggcca
    12481 gtaaatagaa agtttaattt ggcagatgta aagataggaa ctgctgaggg aagctataag
    12541 attcctaatc ttgataaaga ggaagttgaa aagattagag agtttttaag gattaagatt
    12601 aatgaaaacg ttagagagga gctttaggca taattatgaa tgaaaaatta gctaataaag
    12661 aaataagtca atgtaattat gaaaaagaga gccttaataa cagggaaata aaaaatagtg
    12721 taagaagtct actttataat atagttaagg gaatgataag tgattttgta ttaggtctta
    12781 tatttgtagt atttttaaaa caatgggttt acatcttaat aatagtttta gtacttttag
    12841 gaattgcaat ttcaattctt agttggaaga aagagtatat gcttttaaaa ggagatgaag
    12901 ctcattatta taaggggatt ttttctaaaa atactacaat aattccaaag aagagtttta
    12961 aatctatgga tatttctcaa aatttaatag agaggatttt aggatataag attgtaaaaa
    13021 ttgaaagttc ctctagagag gttggagaag aagatattaa aatgagctta agtaatgaag
    13081 atatagcttt gcttaagagc tttgcctttg gagctaacaa agagcttaat aatgaagtaa
    13141 gtgaaggctt tgacttagag gagaatatac ttagaaaaag ttttttagat tctgaatttg
    13201 aaaagttaaa agaggattat gagagtaatt taaataatag gaaaaatata aatgagagtg
    13261 aaaaggtaca tataaataat agtgaagaag taaatattaa agaaaagaaa ataagcaata
    13321 aagatttaat tttatatgga tttacaagct ttaatctatt tatagctatt atttttatat
    13381 ttaatgcttg gggtaagatt gaaaaattta taaattcaga gtatgtggat agtgttataa
    13441 atgggtatat tgcaaatgag gcttctaaaa tagggattat atttgcaata gttggattaa
    13501 tcgtagcttt aattatatta aaagctatag ctacagtata ttattttata aaatattata
    13561 attttaccct tttaaaagaa ggagaaaata taaaaataaa atatggattc tttagcacaa
    13621 aggaattttc ttttaaggaa aatagtataa agcttataaa attaaaatca aatcctttaa
    13681 gacaactttt aaaaagatat gaaataaatg tagttataaa aggctattct ggagagggaa
    13741 aagagcagat aataatgtat cccataggaa atgacaaaga agttcaaaat ataataagag
    13801 aatttatacc tcagtggagc atagaaggtg atggagaagg aataagacat ggaaagatat
    13861 ttatgattct aaaaccagtt ttaatagtat ttattatatc tttagtagcc tatttaatta
    13921 aaagtaaaat gggtattgct tattaatata attagtctta taacaattcc ttcatcaatt
    13981 ttaaaaggaa gaaatataaa tcttaagatt gaggaaaata aagttcgtgc agtaacagga
    14041 ggattcttta gaacaataca tattcttaag gggaaggaca tacaagctgt aggctttaat
    14101 acaaatccca tacaagaaaa aaataatata ggcaaaattg taattgatta ttatagtgaa
    14161 aattcagagg aaataaaatt gccttatatg aataagaatt atgtggaagt actattaaat
    14221 agttctaagg gaattatgca taaaagataa gaataaatga agttaaaaat aaggaaacac
    14281 caccataagt aatgcttcat aattaagtaa gacaagttca tttatattca acaaataagg
    14341 aaacaacagt ttatataatg tgtcagagat aacaattcct agtaatcttt tttctaaaaa
    14401 ttccataaaa ggagaaataa aaaggttgta taagttaatt taactttgca atctttttta
    14461 gttgttttta aattggagat ttaaaaacaa ctataaatat attttaagaa ttatccctaa
    14521 gttcaaaata tataacattg acaattatcg atatataatt ataatgaaat atagttataa
    14581 aaagttttag tattaggaag tgaagaaata tggaggataa gtttgaaaaa aaatcaaggg
    14641 tttttaaagc tttgggagat tcaaatagat taaaaataat agatttgtta tcaagtggag
    14701 aaaaatgtgc ttgtgaaata ttgaagtttt tcgatatatc tcaatctacc ttatcacatc
    14761 acatgaagat tttaagtgaa tgtggacttg ttaaatgtag aaaagaagga acttggaatc
    14821 attatagttt aaatttaaat aatgcaaata aatctatatt gttttttatg gaaataataa
    14881 catgcttaga ttagtatgaa ttatttagga aatatgataa atggtgcaaa ttggtttaag
    14941 ttaattagaa ttgaaatgtt taaaaagata ttagtttatt atgaaaaata gtttagtaaa
    15001 aggggaagaa ttatgaaaaa agtagccttt atatgtgtac acaattcttg tagatcacaa
    15061 atggcagaag cctttggaaa aatactagga ttaaatgttt ttgaaagtta ttccgcaggt
    15121 actgaggaaa gagataaaat aaatcaagat gctgttagaa taatgaaaga aattggagta
    15181 gatatggaat taactcaaag accaaaatta ttaagagaaa ttccagaggt ggatatagtt
    15241 ataactatgg gatgtaatgt ttcctgtcca acattacctt gcaagcatag ggaagattgg
    15301 gggttggagg atccaagtgg aaagagtaat gaagagttta tatacacaag agataaaata
    15361 aaagaaaagg tagaagattt agtaagtcgt ataaaaaatg ggcaaattta aataggagaa
    15421 agggtaaatg aagaaaatag atttaaccaa gggtaatgta ctaagtgtcc ttaccacctt
    15481 agctcttcct attatgggaa gttcattcct tcagtttgct tatgggctta tagatatgtt
    15541 ttgggttgga ggtcttggaa gtagtgcagt ggctagcatt ggctcttcaa gctttttttt
    15601 aggactagga tattctataa atgccttagt agttacggga actggaatta aggtttctca
    15661 tgaagtaggg agaaaaaatg aaatagagac agaggattat ataagaaatg gtattataat
    15721 gaattttctt ataggactaa tatatgcatt agtattagtg gtttttggaa aaggattcat
    15781 aggattttta aatttaaatg atcccttagt tgaaagagat tcttatatat atttagctgt
    15841 aagtgctcca gtattatttt ttagcttttt taattatctt tttactagaa tattagctag
    15901 ctttggtaat aataaattag ctttaaaaat aagtgctaca ggtataattt taaatatgat
    15961 attagatcca atattaatat atacctttaa acttggtgtt tttggagcag cagtggcaac
    16021 cttacttgcc aatatactta tgttttttat gtatatatat tatggaaaag gaatatttaa
    16081 aatagattta aaaaagaaaa taaactatga aaaagtaaga gaaatagcta gattaggatt
    16141 tcctatggca tcacaaaggg tactttttac cctagtgaat attatattag ctagaattat
    16201 agctagcttt ggagcagatg ctatagcagc tcagaaaata gggcttcaaa tagaatctgt
    16261 aagctttatg gtaattggag gattaaatgg ggctatagca ggatttacag gacaaaatta
    16321 tggagcaaag aaactaaata gagtaaatga agggtacaag aaagccttgc ttttaggagc
    16381 tatctactca gtacttgttt cagccttatt tatttttatt ccaggattat tagtaggatt
    16441 atttgttaag gatgaggcta caataataat tggaagcagt tatttaagag tacttggaat
    16501 agttcagata tttagtagtt ttgagatgat atcaaatgga gtattctcag gattaggact
    16561 tcctaaaata ccatcagcca taagtataat ctttactatg ttaagaattc ctatggcctt
    16621 agttttagta aagtttttag ggataaatgg agtttggtgg agcattgcaa tatcaaacat
    16681 attaaaagga agcacatctt gtataattta tagattaaaa atttatagaa actatattgt
    16741 gtaattaata gtatttttca ttatgactgt attaggtaac ttaaattaaa aagtgcttta
    16801 atacagtttt tttatataat aaattaaaga caataaaatc gtaatatata ctaagaaagc
    16861 ttaatacata gctttaaaat tttatctgtt aagaatatta taaaattaat tataatggaa
    16921 ggactggtta gtatgaagag aattcttata attgaagaca cagataccat aagagaagaa
    16981 cttacaaaac ttcttactag atatggatat gaagttctag ctccaacaga ttttgaaaat
    17041 atattagagt atgttaaaga aaataattca gatttaatcc tattagatat aaacttgcct
    17101 tattttgatg gatatcatat ttgtagagaa ataagaaaag attctaatgt gcctataata
    17161 gttacaagta gagatagtga tatggatgaa attatgagca tgaatttagg agctgatgat
    17221 tttataacga agccttataa tactcaagtt ttattagcta gaatagcatc actacttaaa
    17281 agaacctatt ctttatcaga acaagaagtc ttaacacatg gtgatttaaa gcttaattta
    17341 tctaatggaa caatttcttg cagaggaaaa gaagaagagt taactaaaaa tgaacttaag
    17401 atattatcac atcttttaaa aaacaaagga aatgttgttt caagggaaga tcttatggat
    17461 tatatgtgga actcagatat ttttgttgat gataacacac tttcagtaaa tgtaactaga
    17521 cttagaaaaa aattagaaga ggttggagta aaaaattcta tgataacata agcattactt
    17581 tagataaatt agacagaaag tacctattgc ctgaggtaat aggagagtca aatttttatc
    17641 atggtaaatt tttatatgag gttttaaaag aaactgacaa agatatgcat gagcatgtta
    17701 aaaaatatat actggagcaa aaagaatata gagaatatat agaaacatgg gttcatgaga
    17761 taaaaactcc aatagcttcc actaggctta ttttagaaaa taatgaaagt gagtctagtg
    17821 aaaatattaa aaaggaaata aagaaaattg aagaatatat agatcaagct ttatattatg
    17881 ctagaagtac agatgttagc aaagattata ttgtaaaaga attttcttta gggaaaagtg
    17941 taagaaatgt aattaagaaa aattctaggg atttcataaa taagagaata gctatagatt
    18001 tagaagattt agattacaag gtttatagtg atgaaaaatg ggttgaattt ataataaatc
    18061 aagttataga taacgcaata aaatatagtg ctaaaaatag tagagtcaaa atatttgcaa
    18121 agaaatctaa aaatagcata actcttaaaa taaaagataa tggtgttgga atacctaata
    18181 aagacttagg tagagctttt gaaaaaggct ttactggaga aaatggaaga aaatttacta
    18241 aatctacagg tatgtggtta tacctatgca aaaatctttg tgataagtta ggcttaggct
    18301 taaaaataac ttcaaaagaa aattagggaa cagaagttaa catgattttt ccaataggag
    18361 atgccataat agcgaattag atactttata ccgcattatt caaaaatgag tatgcggtat
    18421 ttttatttta atataattaa taagttaaat atataattat attaaaaaaa taagttgtta
    18481 taagaaacta ttatagcttt tttttattat cataatatta tataatgtta aataatgtta
    18541 atgagggtag ggtgatataa ttgggaattt tagatttgtt taaagctaaa gaaaataacg
    18601 agttaaaaga agaaattaaa gaattaaaaa agctattgaa tcctgaacaa caagaaattg
    18661 caaatttagt aaaagaaatt gataaattaa aacttgaaaa agataaaatt tcaaaggagg
    18721 tagataaatt atttagtaaa aagcaagagt tatcaaataa aattaaacta aagaaaaaag
    18781 atttaatact tttagatgat gaaatattaa tgcaagagtt tggattgtat aaacctattt
    18841 atgattttga atcatcagaa aaatataaac aagctataga taatcttaga gttgttcaaa
    18901 aagaaatgat aaaaaataaa agtgcagtta tttattcaaa taactggaca gtagatggaa
    18961 gtaaagccaa aggtagaaaa atgactaatg ataacataaa gcaaattatc atggctttta
    19021 atattgaatg tgataattta attgctaagg taaaatataa taatatacaa agtattcaga
    19081 agagaataga aaagactttt gaaagactaa acaagttaaa tgaaagtaat caagtacgac
    19141 ttactagtaa atatttagaa tgtaaattat cagaacttaa actagtacac gagtatcaag
    19201 taaaaaaaca agaagaaaaa gaagaacaaa aaagaataag agaagaattg cgtgaagaag
    19261 ctaaattgaa gaaggagcta gaagaagcta agaaaaacac tttaaaagac attactcatt
    19321 ttgaaaatgc attatctaaa ttaaatgaac aacttaaatc taataattta tctgatgaag
    19381 aaattaaaaa tttacaactt aaaaaagaag aactagaaaa aaatatagat aatttaaatt
    19441 tgagtttaaa agatatagac tatagacaag aaaatcaaag agctggatat gtttatataa
    19501 tatctaatat aggtgctttt ggtaaagatg tatataagat aggtatgact agacgtttag
    19561 aacctatgga tagaattgat gagcttgggg atgcctcagt gccatttaac tttgatgtac
    19621 atgctatgat ttttgcagat gatgctccaa aattagaaaa tgcactgcat aaagcatttg
    19681 aaaataagaa attaaatatg gtgaatcaaa gaagggaatt ttttaatgtt actttagagg
    19741 aaatagaaaa agtggttaaa gaaaattttg ataaaactgt agaatttaaa aaagaaccag
    19801 aagcagagca gtttagacaa agtttaaaaa ttaaagatac tgtattaact tattaaattt
    19861 taatatataa gataaatatc aataaaaata aaagactaga taaaactctg gtcttttatt
    19921 tagttttagc tatatattcc caaaaaatta aatacttttt gtatatttat aaacttaaat
    19981 aatagattat tataaccagt tatgtataaa ttttgaccag ttatgcaaat tgtattgaaa
    20041 atttcatata ttaaattaat attaaattag tattaactta atattaactt aatgttaatt
    20101 ttcagagggg gatgtatatg tttaagaaaa agttatcatt attgatggct actattacca
    20161 ttggtagcgt tctattagga ggagcttcaa cagtaagtgc tgctccaaga caaaagcata
    20221 aaactgtatc agagaaaatt aaagaggctg aaaagactga aggtgataaa aaactaacgg
    20281 ttatggtata tgctgattgt gataataacc tagaagagta catacttaat gatattgaag
    20341 aaatgaaaga gggttataaa aataatccta atctaaatat tgtagtatta gttgatagaa
    20401 ttcctggtta ttctaatgat tcaaaagtgt taggttctaa ttttgaagac acaagacttt
    20461 ataaaatagg tgaaaattct gctgaaagaa ttagtgggaa aagtgaattc ccagagataa
    20521 ctactacaag taattatgaa gctaacatgg gagatgcaaa tactttaaaa aaattcatta
    20581 aattctgtaa aaagaactat gaagctgata agtatatgtt aatcatgtct aaccatggtg
    20641 gtggggctaa ggatgataaa gatagagcta gtactgtaaa taaggcaata tgctgggatg
    20701 atagtaacaa caaagattgt ctttatactg gagaaatttc agatgtttta actaaggatg
    20761 aatctgttga tgttttagta tttgatgctt gtttaatggg aacttcagag gtagcttatc
    20821 aatatagacc taataatgga agttttgaag ctaaaacact tgtagcatca gcaccagttg
    20881 tttggggaaa tggatatcct tatgataaaa tattttctcg tttaaaatct actaaaggtg
    20941 acaatggaga agttgattct actttaggag gaaaggaaaa aatatttgag ccatcacttg
    21001 ttacaaataa tgaacttggt gccttatttg tagaagaaca acgtgattct gtaaatagct
    21061 atggagtaac agatcaacaa ttaagttgct atgatctttc aaaaattgaa acggttaaaa
    21121 aatcagttga tgctttagct agaaatttaa gcaaaaataa taaaaaagat gctattgaaa
    21181 acttaagagg tacaggaaaa aatgcaccaa ctatgcacta ttttaagaat tatgatgaat
    21241 atgaatggat tgagtatcca tactttgatt tatatgattt atgtgaaaaa attagcttaa
    21301 gtaatgaatt tgatgaaaca actaaaaaat tatcaaagaa tgttatgaaa aatgttgacc
    21361 aattaatttt atattcattt gcaggtaatg actttaaagg cttcaaagaa ggaaaaaatg
    21421 gtataagtat tttccttcct gatggtaata gaaattatta tgatcaatat tcaggacaag
    21481 taataccaca ttgggctatt caaagatggt acaatccttt agacactaat gcttatggat
    21541 taagaagtgg atatggaaaa ctagcatggt gtaaagatgg attagatcca aaaataaata
    21601 aagttggtaa ctggtttgaa cttttagatt cttggttcga taaagataat acttcacttg
    21661 gcggatataa tagatataga tattaaaaga tgatttctta agattatata aaagaaatta
    21721 agttaagaaa taactctact tat
//
LOCUS       DAEVGI010000026        21349 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEVGI010000026 DAEVGI010000000
VERSION     DAEVGI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 21349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21349
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.026"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(96..1433)
                     /locus_tag="K8M77_000903"
     CDS             complement(96..1433)
                     /locus_tag="K8M77_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085415.1"
                     /translation="MDFERTRRKLLNIAMEAEEENKTLTDDFKREFWSLIEKVIISLY
                     DKENSFFGQFLIHVKREIRTDIKWPIATKPEMGYFTMVFNPSIILECDLKEVQALLKH
                     EVYHIMMSHYAREKALSRKYSKLAVSIAMDIAINQYIKNLPPYSKRLDYVNLEYNLEL
                     KPDMPMEKYAEEIQKAIERRKKYGITGDDKNAGDLVSQEKSHEVWEEVSISLDSLEGT
                     TKKTAINAYKGKAPKDLEKIILLMKEKPEIKWSEFLKDIIPTTRGSYRKTITRKDRRL
                     PERLDLRGKLPNSIPKILIAIDVSASISDKEFENIIIEVLGIVRNKNAEIKVIECDDE
                     IRRVYDLKGIKDIKPRSKKNGSTRFSPVFRYIKENNMRNHILVYFTDGVGEKDLEVKP
                     INYRTLWVLTGKKQLSLNKSFGTVKHLEKEKGDGYSLMDGLQDMKDNLHDWAR"
     gene            complement(1447..2562)
                     /locus_tag="K8M77_000904"
     CDS             complement(1447..2562)
                     /locus_tag="K8M77_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7085416.1"
                     /translation="MDFKEALSTVNLVLMTGEVPLLIGESGIGKTSLAKELSRINNYH
                     LVTIDGNLLKEGEIGGLPMVEKKEILFKGDLVKRSVTKYATHVKLMEIDDHIEKGEKV
                     LLFIDELNRCEHAVQQELMNLILNREINGYKLNEDVYILAAMNPSNKYDNYEDSDYQV
                     IEMDPAQEDRFVWIDISSNLKEWMSWAMGDGNIHEHIIEFLSTFPEYFHTPNSLETIK
                     ATPRSWERVSKAYRVYLANKKNFSKEILYNALKGNVGSTIAQEFIAYISNLNKPIISN
                     EEIFESDVLGFNIRERIMSENHSRMYIIAKSALYYLEGLKDYNKELEVFSDLLSCYPK
                     DLRLGIMKEIKSDHSEELYRNLLNEDKFLEAFFEIYS"
     gene            complement(2679..3464)
                     /locus_tag="K8M77_000905"
     CDS             complement(2679..3464)
                     /locus_tag="K8M77_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085417.1"
                     /translation="MNAMNNLELKKIKENPLYIREIENPSEEMQLEAIKKNPFTIQYI
                     ENQCEKCKEEAVRRNPTTIEYVKNPSEEICILAVKSLWNALKLIKEPSEKVVEEAVKT
                     KGWAIQFVKNPSEKLKQIAVSRDYDAIKYIEDPSPEVQLIAVKNHWSAIKFIKNPTLE
                     AMRASVKEDAEAINFLPNYTYEDLETFIGDNINVVKYLYESIDVDMVVDILTKKVAEE
                     NVDERYLLDFIDLEVLEMNKVEFIKEYGSKRAKIILVDSMLSI"
     gene            complement(3737..4528)
                     /locus_tag="K8M77_000906"
     CDS             complement(3737..4528)
                     /locus_tag="K8M77_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBI7085418.1"
                     /translation="MAIQNWKDFLTPYDQAVSELKVKLRSIRKEFRRKNEYSPIEFVT
                     GRVKEVSSILEKANKYSIPVDRIQYEMEDIAGIRIMCQFVDDIDTVVNIIRNRKDMQV
                     LYEKDYVSNVKPSGYRSYHVVIRYPINMADGQKDILAEFQIRTLAMNFWATIEHSLNY
                     KYKKNLPKDIQFKLKRAADAAFTLDEEMLEIKDEIKDAQTLFEVKSDIISTIMNSILN
                     LISIGKVAEASRYQISLNKLIEEGEVWELNNLLRAVQRDIDKYNV"
     gene            complement(4647..5339)
                     /locus_tag="K8M77_000907"
     CDS             complement(4647..5339)
                     /locus_tag="K8M77_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI7085419.1"
                     /translation="MALYAISDLHLALNTDKPMDIFGEKWRNHHEKIKENWNNKITEE
                     DTVLIAGDISWSMKSDESKDDLDWIDALPGKKIIIKGNHDYWWGSISKLNKMYENTKF
                     IQNNFFTYKDWAICGSRGWICEGSDKFTQKDKKIFDREQIRLRLSLEEAKKAGYEKII
                     CMVHYPPTNEKFEDSAFINIFKEFGVDKVIYGHLHGMALKGKVLNEERDGIDYKLTSC
                     DFINFDPIKILD"
     gene            complement(5595..6815)
                     /locus_tag="K8M77_000908"
     CDS             complement(5595..6815)
                     /locus_tag="K8M77_000908"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBI7085420.1"
                     /translation="MANVLDTLMERGYIKQFTHEAETRELLEKEKVTFYIGFDPTADS
                     LHVGHFIAMMFMAHMQKAGHRPIALIGGGTATIGDPSGKTDMRKMMTNETIAHNVACI
                     KKQMEKFIDFSDDKAILVNNADWLLNQNYVEFLREVGVHFSVNRMLSAECFKQRLERG
                     LSFLEFNYMLMQGYDFYVLNKKYNCKMELGGDDQWSNMIAGVELVRKKSQKSAYAMTC
                     TLLTNSEGQKMGKTVNGALWLDPEKTSPYEFYQYWRNVNDADVEKCLKLLTFIPMDEV
                     RRLSSLEGSQINEAKKVLAFEVTKLVHGEEEATKAKQAAEALFGKGGDMSNVPTYEMR
                     KDKLGSELLDILVEAEIVPSKAEGKRLVKQGGLSLNGEKVADFKKTLEEADFENGEVL
                     IKRGKKNYNKIVLA"
     gene            complement(7126..7596)
                     /gene="tnpA"
                     /locus_tag="K8M77_000909"
     CDS             complement(7126..7596)
                     /gene="tnpA"
                     /locus_tag="K8M77_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBI7085421.1"
                     /translation="MDNSSLAHSKWNCKYHIVFAPKYRRQIIYGKIKSDIGAILRKLC
                     DQKGVKIIEANACKDHIHMLVSIPPKLSVSQFMGYLKGKSSLMIFDRHANLKYKYGNR
                     QFWCKGYYVDTVGRNKKAIEEYIKNQIQEDIAYEQISLKEYIDPFTGEPIKKNR"
     gene            complement(7838..8251)
                     /locus_tag="K8M77_000910"
     CDS             complement(7838..8251)
                     /locus_tag="K8M77_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI7085422.1"
                     /translation="MIQLFFNQRGAGKSKNLVKLANEEIEKSRGSIVFIDNDNKRMLQ
                     LNKKVRLIPMDSYCIKSYDQFYGFLQGIISRDYDVESIYIDSIANILDDIKLEDLENY
                     LKKIEIMSRELNVNVFVTIHGDLTIMPENIKKYVA"
     gene            complement(8327..9460)
                     /locus_tag="K8M77_000911"
     CDS             complement(8327..9460)
                     /locus_tag="K8M77_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HBI7085423.1"
                     /translation="MNENKLRKLIKKNEYEKLDFKLKIELFTENVKKELAKDICAIAN
                     SRGGRGYIIIGVEDKTKKIVGVDADYITEERVQQIVASRIEPPVPISLEECFLDGKRL
                     LVIVIFNSYQKPYQIRENGAFYIRRGSTTDIMRKQELLSEFQKGISFNLETCPIVNSN
                     IDFLNEELVKRYFYLKGIKISKENREFLLSSSNIIHKNNISGKSMCTLGGLLVFSDVN
                     SIYIPHNMIKIRNLLGGEQTFIIRGNILDMMKSMEKKIREIVPNEYPVALIIDTIRNI
                     LIYRDYSQYNKIVEVVLGKKELTVYCPGEIVKRYRDGRIGYIKRNMWIYEKIISLDSR
                     GGEMNYNLGEKEVKESFTNKVKVITVNEENITKFIFNKYGTRN"
     gene            complement(9612..10736)
                     /locus_tag="K8M77_000912"
     CDS             complement(9612..10736)
                     /locus_tag="K8M77_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HBI7085424.1"
                     /translation="MNLKKVIASLLSVIIMSIMFIGCYNYKDINKVTFVTSLIFDENE
                     VGNIDIYLDCVKPYRSSNESSDKGRRVLYKGNGKTVLEAIKDINMYSSSKLDFTQCKG
                     YIFTEKAAKNGIRKYTDIINKNQEFMIRPYMFVLFGSPEELLNDVTVDEEYLGVFIDD
                     LVQRMNKSPRVIAIDANDYLELRTNYGNLLVLGALSIRDDMEEKRLELSGGALLKNEV
                     LVRRITAEEGMSYNLLMGDLKRGTLEVMNPQDPNTFITLDISNAKTKTSISYDGENIT
                     LYKDIKVKCLIGESQSRLIVDEKLLNLLELEEEAVIKQYLELFFESFKESDIDIVNVG
                     NLFYRKYPDEVLEKDPISITNLKINVDVEINGTTITGNTL"
     gene            complement(10711..12132)
                     /locus_tag="K8M77_000913"
     CDS             complement(10711..12132)
                     /locus_tag="K8M77_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HBI7085425.1"
                     /translation="MEKQLDFLKDKLKDSFDVKYREVDTALGPATIVFMDVLCSTQFI
                     SEYIVKPLTLVKDGVKDENDIMTKVIDINITNWSKDKNDTLLHVLSGDVVIIFDKFDK
                     VIYCETKGYTRRGVGIPITEAVIKGPREGFNEAFVDNVTLLRRRIKNHNLKFEPLYVG
                     EDTQTVVCISYIKNKAPKELIDEIREKIKNLDYKFILDTNYIEAKLREDRTLFDTVGY
                     TEKADEVAAKLLEGRVAIIVDGTPFVLTVPFFFIENFQTPDDYYLNRYFTSFTRILRW
                     IAFFIAMFLPGIYVALVTHHFSVLPSLFVFRLAVARAGVPFTIVAEVIIMMLLFQIIK
                     EAGLRLPQPIGTSMSLVSGLILGEAAVGAGIASRITIVVVALSAVCYFLIPKLYGAVS
                     IWSIGIVILAALFGIPGVILISVVLLSHLAHLRSCEYQYLFPLGTLSRYKFKDIIIRG
                     RLEEISKDIVGKDGKNEPKESYS"
     gene            12234..13316
                     /locus_tag="K8M77_000914"
     CDS             12234..13316
                     /locus_tag="K8M77_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HBI7085426.1"
                     /translation="MGKLNTRHFIFFIIAAISTSLVNYTSLFIKIGGRDTWIFTIISG
                     IIFFFVTSFIFSVISKIEYYDFKETCYLVLGKSLGNIYILIFSLTLLLMCIESASVSS
                     SSINVNIFAESPIWYCLLFFVITVFLIGKNRFNSILIICIVCISIVLAINLLLALLNI
                     RYIDYTLLLPIFKDRRISEYILCALTQLGSLSSLAIVLPILPRIDDKKNLKKISINTI
                     LLTSIFCTLCIVILISTLGSLRSANVFYPQFIQNQRIYFGGFVENGNIFVMISSTLSW
                     IVKYLITIFSLYTIWKDRVKYKRNFIALISVIVYVFSYLAAKNAYTLFILLRYYQYIL
                     LIVLFICPIIIYTLAYFKSHKFKYLK"
     gene            13471..13626
                     /locus_tag="K8M77_000915"
     CDS             13471..13626
                     /locus_tag="K8M77_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003407210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI7085427.1"
                     /translation="MEDKNLVCKDCGKEFVFTVGEQEFFKEKGFENDPVRCPACRKAR
                     KAQKMNR"
     gene            13879..15537
                     /locus_tag="K8M77_000916"
     CDS             13879..15537
                     /locus_tag="K8M77_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBI7085428.1"
                     /translation="MSNAEHSSNFIRNIIIDDLDSKKHDTIITRFPPEPNGYLHIGHA
                     KSIVLNFELGKEFNGRTNLRFDDTNPIKEDTEYVESIKEDVHWLGYNWSELHFASNYF
                     DEMYKRALLLIKKGKAYVCDLTPEEIKEYRGTLTEPGKESPYRNRSVEENLDLFERMR
                     KGEFEDGSKVLRAKIDMSSPNINFRDPIIYRIAHASHHNTGDKWCIYPMYDFAHPLED
                     AIEGITHSICTLEFADHRPLYDWFVKECEMENIPRQIEFARLNITNTVMSKRKLKQLV
                     DEGIVDGWDDPRMPTIAGLRRRGYTPKSIRNFCKAIGVAKADSTVDYQMLEHFIREDL
                     QETAPRAMAVINPLKLVITNYPEGKSEILEIENNPKDESAGKRAVTFSREIYIEREDF
                     MENPPKKYFRLFPGNEVRLKGAYFVKCNEVIKDENGEVTEIHCTYDPETKSGTGFTGR
                     KVKGTIHWVDSNNCIPAEFRLYEPLILDDCEENEGKHFLEQINPNSLTICKGFIEPSA
                     KDAKPQDKYQLFRHGYFNVDPNFTSDDNLVFNRIVSLKSSFKLK"
     gene            15955..16371
                     /locus_tag="K8M77_000917"
     CDS             15955..16371
                     /locus_tag="K8M77_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBI7085429.1"
                     /translation="MKKLNIIFWSGSGNTEAMANLIAEGAKENGLEVKVLNVSDASTL
                     DVEGCDVLALGSPAMGCETIEEGEMDPFVEEISGLVSGKPTLLFGSYDWGTGEWMDTW
                     KDRMEGYGANVVNTLIVNNTPEGEDIENCKAAGKAL"
     gene            16405..17028
                     /locus_tag="K8M77_000918"
     CDS             16405..17028
                     /locus_tag="K8M77_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBI7085430.1"
                     /translation="MNNDFLNLLETLNNEKYMRSFISYLISPVITGIKPSSTITLSKQ
                     GHNLYKLWDMYGEDFLKDLNLKHILLRETVNSKVVLIYDEINLMNTVYKKSSMDFLEK
                     LDYRLTMSIDEILTHLVNRYDSFHCPHELGIFLGIPVKDVECFMECTKSKCLLCGYWK
                     VYEEEKKAQEIFELYDSSKEIIMNHLLSGKDLKEVYNLTNNVYKFTI"
     gene            complement(17200..17895)
                     /locus_tag="K8M77_000919"
     CDS             complement(17200..17895)
                     /locus_tag="K8M77_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085431.1"
                     /translation="MTNKKVLIVDDEEHIRELIKFNLKKEGYDIEVAVNGTEALNIIR
                     EIKFDLILLDLMLPEIDGLEVCKEIRRNEETSDIPVMMITAKGEEFDKVLGLELGADD
                     YITKPFSIRELMARVKALLRRSNVKKEENIIKFGDVVVNFKTREVTKGTQNVELTLKE
                     FELLKLLIKNKDNILTRELLLDKIWGYEYIGETRTVDVHIRHLRKKIESDDKNPQYIQ
                     TIRGVGYKFTSNE"
     gene            complement(18325..19323)
                     /gene="trpS"
                     /locus_tag="K8M77_000920"
     CDS             complement(18325..19323)
                     /gene="trpS"
                     /locus_tag="K8M77_000920"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBI7085432.1"
                     /translation="MEDKKKIIFSGIKPSGDLTLGNYLGAIKNWTKLQEEYDCYFCVV
                     DLHAITVKQLPADLRRRTLEVLAIYIASGINPEKNTLFIQSHVPAHSEASWLLTCNSY
                     MGELSRMTQYKDKSKKMGDSIGAGLFNYPVLMAADILLYNADLVPVGKDQMQHLELAR
                     DIGTRFNNSYSKTFTIPEGYVPKEGAKIMDLQDPSKKMSKSDANPNGFILIMDEPNVI
                     RKKISRAVTDSIGVVNYTDEQPGVKNLINILCAIKGYTPDEVVKMYDGKGYAEFKNDV
                     AEAIVEELAPVQEKVKALLGDKKALEEIYKKGAEKANYAAMKTLRKMQKKIGLIPR"
     misc_binding    complement(19454..19668)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            20055..21329
                     /gene="brnQ"
                     /locus_tag="K8M77_000921"
     CDS             20055..21329
                     /gene="brnQ"
                     /locus_tag="K8M77_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBI7085433.1"
                     /translation="MNNKKSDFLIIGFALFSMFLGAGNLIFPPLLGRNLGADYYLGII
                     GFIITGVGVPLLGILSSMRVNGKFELFGDKVGKKFSLIYSIALFILIGPLLAIPRTAA
                     TTFELAFAPNFANINYFAVIVVYFLVNLIFVLKPSKVIDVLGKFLTPILLAILFFLIA
                     KGVLSPIDALTKSYTPDALFTSLLEGYQTMDAIAAVIFSSIILGSIKAKGYTGKASFK
                     LLIKAAVIAIGGLAIIYGGLIFLGAHTGDLALDLSNSQLLLFVAESILGHAGAILIGV
                     SMALACLTTSIGLLSAGGSFFERVSNGKLPYKFNVLLMTVTSILVASMGLDKIISLSA
                     TLLGVIYPVTIVLILLNLFHKRITNPLTHKVTVYVALIVSILITLGNFIPGVENLVSL
                     LPLYSKGFGWVVPALIGFIIPSIMPTRETATA"
BASE COUNT         7069 a         3539 c         2383 g         8358 t
ORIGIN      
        1 tcatgattga aatcacgggt gttcttgcca aaggcataaa aacaatatag cacatattga
       61 ttttcaatta aatcaacatg tgctatcttt ttaatttatc tagcccaatc atgaagatta
      121 tccttcatat cttgcaaacc gtccataagg ctatatccat cccctttttc tttttctaaa
      181 tgtttaacag ttccaaaaga tttatttaaa cttaattgtt tcttacctgt taaaacccaa
      241 agagttctat aatttatagg tttaacctct aaatcttttt ctccaactcc atctgtaaaa
      301 tatacaagaa tatgatttct catattattt tcttttatat atctaaatac tggagaaaat
      361 cttgttgagc catttttctt acttcttggc tttatgtctt ttattccttt taaatcataa
      421 actcttctta tttcatcatc acattcaata acttttattt cagcattttt atttcttact
      481 atacctaaaa cttctattat tatattttca aactctttat cagagatgga tgctgaaaca
      541 tctattgcta ttaatatttt tggaatagaa tttggcaatt ttcctctaag gtctaatctt
      601 tctggaagac gtctatcttt tcttgttata gtttttctat aacttcctct tgttgtagga
      661 attatatcct ttaaaaattc actccacttt atctctggtt tttctttcat tagtaagatt
      721 attttttcta aatccttagg agccttgccc ttatatgcgt ttatggctgt tttcttagtt
      781 gttccctcta aactatctaa acttatagaa acctcttccc atacttcatg ggatttttct
      841 tggcttacta aatcaccagc atttttatca tcaccagtaa taccatattt ttttcttctt
      901 tcaattgcct tttgaatttc ttctgcatat ttttccatag gcatatctgg ttttaattct
      961 agattatatt ctaaattcac ataatctagc cttttactat atggaggtaa attctttata
     1021 tactgattta tggcgatatc catagctata ctaacagcaa gttttgaata ttttctactt
     1081 aaagcctttt ctctagcata atgagacatc attatatgat atacttcatg ctttaaaaga
     1141 gcctgaacct cttttaaatc acattccaat attatgcttg gattaaatac cattgtgaaa
     1201 tatcccattt ctggtttagt ggctataggc catttaatat cagttcttat ttctctcttt
     1261 acatgaatta aaaactgacc aaaaaatgaa ttttctttat cataaagaga gattattacc
     1321 ttctctatta aactccaaaa ttctctctta aaatcatctg ttaaagtctt attctcctct
     1381 tcagcttcca ttgctatatt aagaagtttc ctcctagttc tttcaaaatc cataagattt
     1441 caccacctag ctatatatct caaagaatgc ttctaagaac ttatcctcat ttaataaatt
     1501 tctatataat tcttctgagt gatcgctttt tatttccttc attattccaa gtcttaaatc
     1561 cttaggataa caagataata aatctgagaa cacttctaat tctttattat aatcctttaa
     1621 tccctcaaga taatataaag cactcttagc tattatatac attcttgaat gattttcact
     1681 cattattctt tctctaatgt taaatcctaa aacatcactt tcaaaaattt cttcattaga
     1741 aataataggc ttattaagat tacttatata tgctataaat tcttgtgcta ttgttgagcc
     1801 tacatttccc tttaaagcat tatataatat ttccttagaa aagtttttct tatttgccaa
     1861 atacactcta taagccttag aaaccctctc ccagcttctt ggagtagctt ttattgtttc
     1921 tagcgaatta ggagtgtgga aatattcagg gaatgttgat aaaaactcta ttatatgttc
     1981 atggatattt ccatctccca ttgcccaact catccactct ttaagatttg aactaatatc
     2041 tatccaaaca aatctatctt cttgggctgg atccatttca ataacctgat aatcactatc
     2101 ttcatagtta tcatatttat ttgatggatt catagctgct aatatatata catcctcatt
     2161 taacttatat ccatttattt ctctatttaa tataagattc ataagttctt gttgcactgc
     2221 atgctcacat ctatttaatt catcaataaa taaaagaact ttttctcctt tttcaatatg
     2281 atcatcaatt tccataagtt ttacatgtgt agcatatttt gttacgcttc tttttactaa
     2341 atctccttta aataggattt ctttcttctc taccattgga agaccaccta tttctccctc
     2401 tttaagaaga tttccatcta tagtaactaa atgataatta tttattctac taagttcctt
     2461 tgctaaagaa gtttttccta taccactttc tcctattaaa agaggtactt ctcctgtcat
     2521 aagaactaaa ttaactgtac ttaacgcttc tttaaaatcc actttagtgt cccttctttc
     2581 tataaatctc tccgtgaaac ttaccttata acctctcttg tgaaaaatct aaaatatttt
     2641 gtaaactaat ctatatttat atttttaaat ctctattttt atattgataa catactatca
     2701 actaatatta tctttgctct tttacttcca tattctttga taaattctac tttgttcatc
     2761 tctaagactt ctaaatcaat aaaatctaaa agatatcttt catctacatt ctcttctgca
     2821 actttttttg taagtatatc aacaaccata tcaacatcta tactttcata aaggtattta
     2881 acaacattta tattatctcc tataaaagtt tctaaatctt cataagtata gtttggtaag
     2941 aagtttattg cctcagcatc ttccttaact gaagctctca ttgcttccaa agtaggattt
     3001 tttataaatt taattgctga ccaatgattt ttaacagcta taagctgaac ttctggtgat
     3061 ggatcttcta tatactttat tgcatcataa tccctagaaa cagctatttg ttttaacttt
     3121 tctgaaggat ttttcacaaa ttgtatagcc caccccttag ttttaacagc ttcttcaacc
     3181 accttttctg aaggttcctt aataagcttt aaggcattcc atagagattt tactgctaat
     3241 atgcatattt cctctgatgg atttttaaca tactctatag tagttggatt tcttcttaca
     3301 gcttcctctt tacatttttc acattgattt tcaatatatt gaattgtaaa tggatttttc
     3361 ttaatagcct ctagttgcat ctcctctgat ggattttcta tttctcttat atataaagga
     3421 ttttccttaa tcttttttaa ttctaaatta ttcatagcat tcataaatta cacttcctca
     3481 attttatttt tctctcaata aatgctcaat cattttaaaa ctcaaataat gtgttgctcc
     3541 gttaaagttg agtaattcta tcaactttct aaattacatt tattattata taacaattat
     3601 tattaaatat aaagacataa aataaaattt taaaataaac aataaaattt cttttccaaa
     3661 ttaattttcc ctaaatattt atataaaaat accaccttta aataaggtgg cattaaaaac
     3721 tacttttatt taaatattat acattatact tatctatgtc tctttggaca gctctcaata
     3781 aattattaag ttcccaaact tctccctctt caataagttt atttaaactt atttgatatc
     3841 tagaagcctc agccactttt cctattgaaa taagatttaa tatactattc attatagtac
     3901 ttataatatc tgatttaacc tcaaataaag tttgagcatc ctttatttca tcttttattt
     3961 caagcatttc ttcatctaaa gtaaatgctg catcagctgc acgttttaat ttaaattgta
     4021 tgtcctttgg cagattcttt ttatacttat aatttaaaga atgttctatt gtagcccaga
     4081 aattcattgc taaagttctt atttggaatt cagcaagtat atctttttgt ccatctgcca
     4141 tatttatagg atatcttata accacatgat agcttctata gccacttggt tttacattgc
     4201 taacatagtc cttttcataa agaacttgca tatcttttct attccttata atattgacaa
     4261 cagtatctat atcatcaaca aattggcaca ttattcttat acctgcaata tcctccatct
     4321 catattggat tctatctaca ggaatactat atttatttgc cttttctaat atactagata
     4381 cctctttcac ccttccagtt acaaactcga ttggagaata ttcatttttt cttctaaatt
     4441 cttttctaat acttcttaac tttaccttta attcacttac tgcttgatca taaggagtta
     4501 aaaagtcttt ccaattttgt atagccatag aaccacctat ttcctaactc tcttagagag
     4561 atttatttaa ctctcctcgc tcattatacc aaaaaaagta ttaaaactaa atatttcaaa
     4621 atcttaattt tacatgaaac agtgtcttaa tctaagattt ttattgggtc aaaatttata
     4681 aaatcacagc ttgtaagctt ataatctatc ccatctctct cttcatttaa taccttgccc
     4741 ttaagtgcca taccatgaag atgaccataa ataactttat caacaccaaa ctctttaaat
     4801 atattgataa aggctgaatc ctcaaatttc tcatttgtag gtggataatg taccatacat
     4861 attatctttt catatcctgc ctttttagct tcttctaaag ataatcttaa tcttatctgt
     4921 tccctatcaa aaatcttttt atccttttgt gtgaatttat cagaaccttc acaaatccac
     4981 cctctgcttc cacatatagc ccaatcttta tatgtaaaaa aattattttg tatgaacttt
     5041 gtattttcat acattttatt caatttagaa atacttcccc accaataatc atgattccct
     5101 ttaattatta tctttttacc cggaagtgcg tctatccaat ctaaatcatc tttactttca
     5161 tctgatttca ttgaccaaga aatatcccct gctattaaaa cagtatcttc ctcagtaatt
     5221 ttattattcc aattttcttt tatcttctca tgatgattac gccacttctc accaaagata
     5281 tccattggtt tatctgtatt aagtgctaaa tgcaaatcag agatagcata taacgccata
     5341 tttcatcacc tttcatatat ctatttagtc ttacatgtaa ttattagaga aaatattttc
     5401 tttagtaaat aaaataaatc atctaaacta atctacattt tgtcagtaat ttattataca
     5461 cttttattaa ttatatatac aagtttcaaa gcggtagaaa taaaactgga gattaaaaat
     5521 aaaaagagtg taaatagaat atattaccca taataattag ctaatatatt ctaatacact
     5581 cttctaaaaa agaattatgc taaaactatt ttattgtagt ttttcttacc tcttttaatt
     5641 aaaacttcac cattttcaaa gtcagcttct tctaatgtct ttttaaaatc agcaactttt
     5701 tctccattta atgataatcc accttgctta acaagtcttt ttccttcagc ttttgatgga
     5761 actatttcag cttcaactaa aatatctaaa agttctgaac ctaacttatc ttttctcatt
     5821 tcataagttg gaacattact catgtctcca ccttttccaa ataaagcttc agcagcttgc
     5881 tttgcctttg tagcttcctc ttcaccatgt acaagtttag ttacctcaaa tgctaaaact
     5941 tttttagctt catttatttg agaaccttct aaacttgaaa gtcttctaac ttcatccata
     6001 ggaataaatg ttaatagttt taagcatttt tcaacatctg catcattaac atttctccag
     6061 tattggtaga actcgtatgg agaagttttc tctggatcta accataatgc accattaact
     6121 gttttaccca ttttttgtcc ttctgagtta gttaataatg tacaagtcat agcatatgca
     6181 cttttttgtg atttctttct tacaagttca acaccagcta tcatgtttga ccattggtca
     6241 tctccaccta gctccatttt acagttatat tttttattta aaacatagaa gtcatatcct
     6301 tgcattaaca tgtagttaaa ttctaagaat gaaagacctc tttcaagtct ttgtttaaaa
     6361 cattctgctg aaagcattct attaactgag aagtgaactc caacctctct taagaattca
     6421 acatagtttt gatttaataa ccaatcagca ttgttaacaa gtatagcttt atcatctgaa
     6481 aaatctatga atttctccat ttgctttttg atacaagcaa cattatgagc tatagtctca
     6541 tttgtcatca tctttctcat atcagtctta ccacttggat ctccaattgt agctgtacct
     6601 ccaccaatta aagctatcgg tctatgtcca gccttttgca tatgagccat aaacatcatt
     6661 gcaatgaagt gtcctacatg taaactatca gctgttgggt caaatcctat ataaaaagta
     6721 actttttcct tctctaaaag ttctcttgtt tcagcttcat gagtaaactg ttttatgtac
     6781 ccacgttcca ttaacgtatc taatacatta gccatttgtt tatccctcca aaaaattcta
     6841 atccagctta cttgctggtg ctatatttag caatcatata gtatatagta tatcctttat
     6901 tatattaatt ctcaattgat tttaaaaaaa agctattttt ttaattatac aaatctaaat
     6961 aactacccaa aagtaaggct cactaaaaat gtataatcat aaccacccgt aaaacgggtg
     7021 gtttgctcta gccctataag ggcatgttac tggctgtgct actcgcactt tgaaagatct
     7081 gccaaccgca ttttttacac taactacccc ttaaaggggt ttttgttatc tgtttttctt
     7141 tatcggctca cccgtaaacg ggtcaatata ctcttttaaa cttatttgct catatgctat
     7201 atcttcctgt atttgatttt taatatattc ttctatcgcc tttttatttc ttccaactgt
     7261 atcaacataa tatcctttac accaaaattg tctattccca tacttatatt ttaagttagc
     7321 atgtctatca aaaatcatca aagaactttt ccctttcaaa tatcccataa attgagaaac
     7381 acttagtttt ggtggaatac ttacaagcat atgtatatgg tctttacatg catttgcttc
     7441 tattattttc actccttttt gatcacataa ctttcttaat attgctccta tatctgattt
     7501 tatttttcca taaattattt gtcttctata cttcggtgca aaaactatat ggtatttgca
     7561 attccacttg ctatgtgcta agctactatt gtccatttcg gacacctcct tgtaaaatta
     7621 agttttggtc ggcaaaccta ctattatttt acatcggagg ttttattttt ttctactcat
     7681 agctataagc tttttagaac cacaggtaga acctgtggta ttcattaata caaaaaaata
     7741 agaaccataa ttttaaaatt atagttctta ttatgccaat agtaataaat actatataaa
     7801 tctatataaa ataatttatt ttctaaacta gctaagctta agccacatat ttcttaatat
     7861 tttcaggcat tatagttaaa tctccatgaa tagttacaaa tacgtttaca ttaagttctc
     7921 tactcataat ttctattttt ttcaaataat tttctaaatc ctctaatttt atatcatcca
     7981 aaatatttgc tatgctatca atatatatac tttctacatc ataatctcta gaaattatac
     8041 cttgtaaaaa cccataaaat tgatcataac tctttataca ataactgtcc ataggtataa
     8101 gtctaacctt tttatttaac tgaagcattc ttttattgtc attatcaata aaaacaatac
     8161 tacctctaga tttttcaatt tcttcattag ctagtttaac taaatttttg ctctttccag
     8221 cgcctctttg gttaaagaat aattgaatca tccttaccac cccttaataa tatttaatta
     8281 ggtaaaaact tactttatat ataatattct taatatcata aaaatattaa tttcttgttc
     8341 catatttatt aaatataaat ttagttatat tttcttcatt aactgttata actttcacct
     8401 tattagtaaa gctctccttt acttcttttt cacctaaatt gtaattcatt tccccacctc
     8461 tactgtctaa gcttattatt ttttcatata tccacatatt tctctttata tatcctatac
     8521 gtccatctct atatcttttt acaatttctc ctggacaata tactgttaac tctttctttc
     8581 ctaagaccac ctcaacaatc ttattatatt gagagtaatc tctgtatatc aaaatatttc
     8641 ttatagtatc aattattaag gctacaggat attcattagg tactatttct cttatttttt
     8701 tctccatact tttcatcata tctaaaatat ttcctcttat tataaaagtt tgttctccac
     8761 ctaataaatt tcttattttt atcatattat gtggtatata aatactattt acatctgaaa
     8821 aaactaataa tccacctagt gtacacattg attttccaga gatattattt ttgtgaatta
     8881 tattgctact actaagtaaa aattctctat tttccttaga tattttaata ccctttagat
     8941 aaaaatatct ttttactaat tcctcattta aaaagtctat attactattt actatggggc
     9001 atgtctccaa attaaagctt ataccttttt gaaattctga taaaagctct tgttttctca
     9061 taatatctgt agttgaccct cttcttatat aaaaggctcc attttccctg atttgataag
     9121 gcttttgata gctattaaat attactatta ccaataatct ttttccatct aaaaaacatt
     9181 cctctaaact tatgggaaca ggtggttcaa tccttgaggc aactatttgt tgaactcttt
     9241 cttcagtaat ataatcagca tccacgccaa ctattttctt tgttttatcc tcaaccccta
     9301 ttattatata acctcttccc cctctagaat tagctatagc acatatatcc tttgcaagtt
     9361 cttttttcac attttctgta aaaagctcta tctttaactt aaaatctaat ttttcatatt
     9421 catttttctt tattaatttt ctcagtttat tctcattcat aataccctct tctttaataa
     9481 atattattac ccttatacct taaataaaat atggcataaa atcaaagccc atagaacatt
     9541 atattccata ggctttttaa attgattgta tttatagttt aagaatttat ttattaaatt
     9601 ataaacttat tttaaagtgt atttcctgta attgttgtgc cattaatttc aacatcaaca
     9661 ttaatcttta aatttgtaat gcttatagga tccttttcta atacctcatc tggatatttt
     9721 ctataaaaaa gattgccaac attaacaatg tctatatcac tctctttaaa gctttcaaag
     9781 aaaagttcta agtattgttt tattactgcc tcttcttcta actctaaaag atttaaaagt
     9841 ttttcatcca caatgagtct tgattgtgat tcaccaatta aacatttaac ctttatatcc
     9901 ttataaagag ttatattttc tccatcataa cttattgatg tctttgtttt tgcattagat
     9961 atatctaatg ttataaaagt attagggtct tgagggttca taacttctag agttcctctt
    10021 tttaaatctc ccattaaaag attatagctc ataccttctt ctgcagttat ccttctcact
    10081 aagacctcat tttttaacaa agctcctcca cttaactcta atcttttttc ttccatatca
    10141 tctcttatac ttaatgctcc taaaactaat agatttccat aattagttct aagctctaaa
    10201 taatcattgg catcaatagc tataactcta ggacttttat tcatcctttg aactaaatca
    10261 tcaataaaaa cacctaaata ctcttcatca acagttacat catttaaaag ttcttctgga
    10321 gaaccaaata aaacaaacat ataaggtcta atcatgaatt cttgattttt atttataata
    10381 tctgtgtact ttcttattcc atttttagca gccttctctg taaatatata tcccttgcat
    10441 tgagtaaagt ctaacttact actagaatac atatttatat ccttaattgc ttctaaaact
    10501 gtttttccat tacctttata taatactctt cttcctttat cagaactttc atttgaactt
    10561 ctataaggtt ttacacaatc taaatatatg tctatatttc ccacttcatt ttcatcaaat
    10621 attaagcttg ttacaaaagt aactttattt atatccttat aattataaca accaataaac
    10681 atgattgaca taattattac gcttagtaaa ctagctataa ctttctttag gttcattttt
    10741 accatcctta cctactatat cttttgatat ttcttctaat cttcctctta taataatatc
    10801 tttaaatttg tatctactta aagttcctaa tggaaataag tattgatatt cgcaacttct
    10861 caaatgtgct aagtgagata ataatacaac acttattaaa atcacaccag gaattccaaa
    10921 taaggcagct aaaattacta ttcctatact ccaaatcgat actgcaccat aaagcttagg
    10981 aatcaagaaa taacaaacgg cacttaaagc aactactaca atagtaattc ttgaagcaat
    11041 tccagctccc acagcagcct ctcctaaaat aagtcccgat actaaactca tagatgttcc
    11101 tataggctga ggtaatctta gacctgcttc ttttattatt tggaataata acatcattat
    11161 tatcacctct gctactattg taaaaggaac tccagctcta gcaactgcta acctaaaaac
    11221 aaataaacta ggcaaaaccg aaaaatgatg agtaactaat gcaacatata ttcctggtaa
    11281 aaacatagca ataaaaaatg ctatccatct taaaattcta gtaaaactag taaaatacct
    11341 atttaaataa taatcatctg gtgtttgaaa attttctata aaaaagaaag gtactgtaag
    11401 aacaaaagga gttccatcta ctattattgc aacccttcct tctaatagtt ttgctgctac
    11461 ctcatctgct ttttctgtat atccaacagt atcaaataaa gttctatctt ctctaagttt
    11521 agcctcaata taattagtat ctaatataaa cttataatct aaatttttta ttttttctct
    11581 gatttcatca attaattcct ttggagcttt attttttata taagatatac atactacagt
    11641 ttgagtatct tcccctacat ataaaggctc gaattttaga ttatgatttt ttatccttct
    11701 tcttaataag gtaacattat caacaaaagc ctcattaaaa ccttctctag gtccctttat
    11761 tactgcttcc gttattggaa tcccaacacc tcttcttgta taaccctttg tttcgcaata
    11821 aattacctta tcaaacttat caaaaattat tactacatcc cctgataata catgaagtaa
    11881 tgtatcgttt ttatcctttg accaatttgt tatgtttata tctataactt tagtcataat
    11941 atcattttca tcttttactc catccttaac taaagtcaat ggttttacta tgtactcact
    12001 tatgaactga gtagaacata aaacatccat aaaaacaatt gtggctggtc ctaaagctgt
    12061 atccacctcc ctatacttaa catcaaaact atcttttaat ttgtctttta aaaaatctaa
    12121 ttgtttttcc attcatttat cacccaaaaa tattatccgc tagtattatg tttaatattc
    12181 atttttttga gaatactaaa atttgaaatt ataaaagaaa tgaggtgatg tctttgggaa
    12241 agctaaatac aagacatttt atatttttta tcattgctgc cataagtaca tctttggtta
    12301 actatacatc cttatttata aaaataggcg gaagagatac ttggattttc actataatat
    12361 ctggaattat atttttcttt gtaacttcct ttatttttag tgtaatatca aagatagaat
    12421 attacgactt taaagaaact tgctatctag ttcttggtaa atcattagga aatatttata
    12481 tattaatatt ttcactaact ttattattaa tgtgcataga atcagcttct gtaagctcat
    12541 cttctataaa tgttaatatt tttgcggaga gtccaatatg gtattgttta ttgtttttcg
    12601 taataactgt atttttaata ggtaaaaata gatttaatag catacttata atatgtatag
    12661 tttgcataag tattgtactt gcaattaatt tgttattagc attattaaat attagatata
    12721 tagattacac acttcttctt cctatattta aggatagaag aatttcagaa tatattcttt
    12781 gtgctttaac tcaattaggc tcattatcaa gcttagctat agttttacct attcttccaa
    12841 gaatagatga taagaaaaac ttaaaaaaga tctcaataaa cacaatactt ttaacctcta
    12901 tattttgtac tttgtgtatt gtcatactaa tatcaaccct aggttctttg aggtctgcaa
    12961 atgtttttta tcctcaattt atccaaaacc aaagaattta ttttggtggt tttgtagaga
    13021 atggaaatat ttttgtaatg atttcttcta ctctttcttg gattgtaaaa tatttaataa
    13081 ctattttttc attgtataca atttggaaag atagggtaaa atataaacgt aattttatag
    13141 cattaatttc agtaatagta tatgtctttt cttacttagc cgctaagaat gcctataccc
    13201 tttttatact attaagatac tatcaataca tacttctaat agtgctgttt atatgcccta
    13261 taatcatata tacactagca tattttaaga gtcataaatt taaatatttg aagtaaaaag
    13321 tattgtattc ttttttctat atgatataat caatatataa aataaaattt agtagatatg
    13381 tactttattt ctgtcctgta aaatttattt tattagtttt atagtataga tctcaaacgt
    13441 acctagtaaa ttttacagga ggtaaaagaa atggaagata aaaatttagt ttgtaaagac
    13501 tgtggaaaag aattcgtatt cactgtagga gaacaagaat tcttcaaaga aaaaggattt
    13561 gaaaacgatc cagttagatg cccagcttgc agaaaagcaa gaaaagctca aaaaatgaat
    13621 agataattga taattaacgc aagtttattc ataaattaaa agttctgcac tttgcagaac
    13681 ttttttcatt tctatttcat aaaattaatc ttatttggaa atattatata gagcaattta
    13741 acttatgatt tttatacata attaaatata attttattaa aaactatata ctcatttttt
    13801 aaatatatta tggatagatt ttgcttttta tgatatattt atgagtatat aaaaatgaga
    13861 gaaaagggat gataaacaat gtcaaatgct gagcactcat caaactttat aagaaatata
    13921 ataatagatg atttagatag caaaaaacat gatacaataa taactcgttt tccacctgaa
    13981 cctaatggat atttacatat aggtcatgca aagtctatag ttttaaactt tgaattagga
    14041 aaagagttta acggaagaac taacctaaga ttcgatgata caaatccaat taaagaggat
    14101 actgaatacg ttgaatctat taaagaagat gttcattggt taggttataa ctggagcgaa
    14161 ttacattttg catcaaacta ctttgatgaa atgtataaaa gagctttact tttaattaaa
    14221 aaaggtaaag cttatgtttg tgatttaact ccagaagaaa tcaaagaata tagaggtact
    14281 cttactgagc ctggtaagga aagtccatat agaaatagat cagttgaaga aaacttagat
    14341 ctatttgaaa gaatgagaaa aggtgaattt gaggacggct caaaagtttt aagagctaaa
    14401 atagatatga gctcacctaa cataaacttt agagatccta taatttatag aattgctcat
    14461 gctagtcacc ataatacagg tgataaatgg tgtatctacc ctatgtatga ctttgctcat
    14521 ccattagaag atgctattga aggaataact cactctattt gtactttaga atttgctgat
    14581 cacagaccat tatatgattg gttcgttaaa gaatgtgaaa tggaaaatat tccaagacaa
    14641 attgagtttg caagacttaa cattacaaat actgttatga gtaaaagaaa attaaaacaa
    14701 ttagttgatg aaggcatagt tgatggttgg gatgatccaa gaatgcctac tattgccgga
    14761 cttagaagaa gaggttacac tcctaagtca ataagaaact tctgtaaagc tataggcgtt
    14821 gctaaggcag attctactgt tgattatcaa atgcttgagc atttcataag agaagacctt
    14881 caagaaactg ctccaagagc tatggctgtt ataaatccac ttaaattagt tataacaaac
    14941 tatcctgaag gaaaatcaga gattcttgaa atagaaaata atcctaagga tgaatcagct
    15001 ggtaaaagag ctgttacatt ctcaagagaa atttatattg aaagagagga tttcatggaa
    15061 aatcctccaa agaaatattt cagattattc ccaggaaacg aagtaagatt aaaaggtgct
    15121 tattttgtaa aatgtaacga agtaataaaa gatgaaaatg gggaagttac agaaattcac
    15181 tgtacttatg acccagaaac taagagtgga actggattta ctggaagaaa agttaaagga
    15241 acaatccact gggttgattc taataactgt attcctgctg aatttagatt atatgaacca
    15301 ttaatcttag atgattgtga agaaaatgaa ggaaagcact tccttgagca aataaatcct
    15361 aactcattaa ctatatgtaa gggattcatt gagccaagtg ctaaagatgc taaacctcaa
    15421 gataaatatc aattatttag acatggatac ttcaatgttg atcctaattt cacaagtgat
    15481 gataatttag tattcaacag aatagtttca ttaaagagtt catttaaatt aaaataataa
    15541 aaaataatat ttaagggcat gtatcaaaat attttttaat aaaacttata actctattct
    15601 atttataatg tttaaaagtt ttattattaa tatttgatac tgcccttttt cttatttaaa
    15661 tctactaata aattttactt gtatttattt tctataattt aagttaaata ttttctatgt
    15721 tccttaatcc ccttaaagat ctattataaa tttcctatca ctttactttt caaagaagtc
    15781 taataaaatt tatttttctt tcttctaatt cattcaaaaa aagatttcac tatttattaa
    15841 aaattattat catttactat tgacattatt atcttactat tgtattataa tgatataaac
    15901 taattgaaat tgattttcat taataataag aattgaattt acgaggtgat atatatgaaa
    15961 aaattaaata ttattttttg gtcaggttct ggtaatacag aagccatggc taacttaata
    16021 gctgaaggag ctaaagaaaa cggattagaa gtaaaagttt taaatgtttc tgatgcatct
    16081 actctagatg tagaaggatg tgatgtatta gctttaggtt ctcctgctat gggatgtgaa
    16141 actattgaag aaggcgaaat ggatccattt gttgaagaaa tttcaggact tgtatctgga
    16201 aaaccaactt tattattcgg ctcatatgac tggggaactg gcgaatggat ggatacttgg
    16261 aaagatagaa tggaaggcta cggtgcaaat gtagtaaata cattaatagt taataatact
    16321 ccagaaggag aagacataga aaattgtaaa gctgctggta aagcacttta agatttattt
    16381 aaaagaaagg tggaagaagc tgaaatgaat aatgatttcc ttaatctctt agaaactctt
    16441 aacaacgaaa aatatatgag atcctttata tcatatttaa tttctcctgt aataacaggg
    16501 attaagcctt cttccaccat tactctttca aagcaaggtc ataatttata taagctttgg
    16561 gatatgtatg gtgaagattt cttaaaagat cttaatttaa aacacatatt gcttagagaa
    16621 actgtaaatt ctaaggtagt attaatttat gatgaaataa atttaatgaa tactgtatat
    16681 aaaaaatctt ccatggattt tttagaaaaa ctggactata gattaactat gagcattgat
    16741 gaaatattaa ctcatttagt taatagatat gattcattcc attgtcctca tgaattaggt
    16801 atttttctag gaattccagt taaggatgta gaatgcttta tggaatgtac taagagcaaa
    16861 tgcctccttt gtggatattg gaaggtatat gaagaagaga aaaaagccca agaaatcttt
    16921 gaactttatg atagttcaaa agaaataata atgaatcact tgctttctgg taaggattta
    16981 aaggaagttt ataacttgac aaataatgta tataaattta ctatttgact ctcattgcta
    17041 ataaaccact caaatttctg ccattatctg agtggtttta tttttttctt atttttgctc
    17101 taaatttact acacctataa attgctcttc atatatgcaa tttatttttt taatatacta
    17161 ctttaataag taattataag tttatccttt acattgtaat tattcattgc ttgtaaactt
    17221 atatccaact cctcttatag tttgaatata ttgaggattt ttatcatcac tttcaatctt
    17281 tttacgtaaa tgtctaatat gaacatctac tgttcttgtt tcacctatgt attcatatcc
    17341 ccaaatttta tcaagtaaaa gctctctagt taatatattg tccttattct ttataagaag
    17401 ctttaataat tcaaactcct ttaatgttaa ttctacattt tgagttcctt ttgttacttc
    17461 tcttgtttta aaattaacta ctacatcacc aaatttaatt atattttctt cttttttaac
    17521 attacttctt ctaagtaaag cttttactct agccataagt tctcttattg aaaatggctt
    17581 tgttatataa tcatctgctc caagttctaa gcctaatact ttatcaaact cttccccctt
    17641 tgctgtaatc atcattactg gaatatctga agtttcttca tttcttctaa tttctttaca
    17701 tacttctaag ccatctattt ctggaagcat taaatctaaa agtattaaat caaacttaat
    17761 ttctcttata atatttagag cttcagttcc attaacagca acctctatat catatccctc
    17821 tttttttaag ttaaatttta taagttctct aatatgttcc tcatcatcta ctattaggac
    17881 ttttttattt gtcattcttg tccctccttt acccaaaaca ctaccctatt ttatagctta
    17941 tcataatatt taatacaaaa aaagacatac aaattatata gtcatcctta aattattttg
    18001 ctaattaatt ttctttattg tataattttt acaaattaaa ggaaataaca atttaaaaca
    18061 tataataagt tcaataaata taaactaact aataaaaagc tctataaaaa agttcactag
    18121 atataagcga agtaatattt gaataagtga ttagaatttc atttattctg taataaatat
    18181 aatgttaaaa ggctgagact gtttgaccaa cgggagtttc gaagccttaa ttatatttat
    18241 ggaagaatta atagaaattc ttcacgaaat tcaataatta ctaagctata tctagtgaac
    18301 tttacttaat agagttctta attcctatct tgggattaat cctattttct tttgcatttt
    18361 tctaagagtt ttcattgcag cataatttgc tttttctgca ccctttttgt atatttcctc
    18421 taaagctttt ttatctccta ataaggcttt aaccttttct tgaacaggag ctaactcttc
    18481 aactatagct tcagctacat catttttaaa ttcagcatat cctttgccat catacatttt
    18541 tacaacttca tcaggagtat atccttttat tgcacaaaga atatttatta aatttttaac
    18601 tcctggctgt tcatcagtat aatttacaac acctatacta tctgttacgg ctcttgaaat
    18661 tttctttctt ataacgttag gttcatccat tattaatatg aaaccatttg gattagcatc
    18721 tgatttagac atctttttgc ttggatcttg taaatccatt atttttgctc cctcttttgg
    18781 aacatatccc tctggtatag taaatgtttt gctataagaa ttgttaaatc tagtaccaat
    18841 atctctagca agctctaaat gttgcatttg gtcttttcca actggaacaa ggtctgcatt
    18901 atataaaagt atgtctgctg ccataagaac tggatagtta aataatcctg cccctattga
    18961 atcgcccatt ttctttgatt tatctttata ctgagtcatt ctgcttaatt cacccatata
    19021 tgaattacat gttaaaagcc atgaggcttc tgaatgagct ggtacatgtg attgtataaa
    19081 taatgtattt ttctctggat ttattcctga tgctatatat atagctaaaa cttctaaagt
    19141 tcttcttctt aaatctgctg gtaattgttt tactgttatg gcatgtaaat caacaacgca
    19201 gaaataacaa tcatattctt cttgtaattt agtccaattt tttatggctc ctaaataatt
    19261 tcctaatgtt aaatcacctg atggctttat tccactaaaa ataatttttt tcttatcttc
    19321 cattagtttt tctgatgctt ctaaaaatta attgaagtat cagatacacc tcctttataa
    19381 aaatttaatt aatcttttta tttcttaaat actgttttct tttaaaataa aaaagcccta
    19441 taagccataa gacttatagg acgaatatat cgcggtacca cctaatgtta caaagaaaat
    19501 tctttgtatc tctttcttgt aacgtaagaa ttacgattta gaatactaaa attcctctaa
    19561 acatctcaaa gatccattca acttaagcat aactatcata ctcccagcaa acatatgact
    19621 ctcttaaagt ttgtttttaa gtttactaat tcttgtcatt gatattttat attaacttta
    19681 atgttatttt atcatttttt attgtttagt caagattatt taatttcttt acttattatt
    19741 aatatgaatt ttataattac ccttttaatg ttcattttga cattatttta atattatagt
    19801 tattttttta ttaaattatc aattttatat tttcataatt ttatatataa aatatttttg
    19861 ttgatttttc ttaacattat gataatatag tattataacc tttaaaataa gaatttaata
    19921 aaagaatatt aacaaaacac tatataaaaa attacaaatc aaattatttt aaaattattt
    19981 accttttata ttttaaataa taatttttaa taaaaatatt aatttggttg cttatgaaag
    20041 acggggtgta tattatgaat aacaaaaaat cagatttttt aataatcgga tttgcactat
    20101 tctcaatgtt tttaggggct ggtaacctta ttttcccacc tttattagga agaaatttag
    20161 gtgcagatta ctatttaggg attataggat ttataataac tggagttgga gtaccactac
    20221 ttggtatatt atcttcaatg agagttaacg gtaaatttga attattcgga gataaagtag
    20281 gtaaaaaatt ctcattaata tattcaatag cattatttat acttattgga ccattattag
    20341 ctattcctag aacagcagct actacatttg aattagcatt tgctcctaac tttgcaaata
    20401 taaattactt tgcagtaata gttgtttact ttttagttaa tttaatcttt gtattaaaac
    20461 catcaaaagt tatagatgtt ttaggtaaat ttttaactcc tatattatta gctatattat
    20521 ttttcttaat agctaaggga gttcttagtc caatagacgc tttaacaaaa tcatatactc
    20581 ctgatgctct ttttacatca ttacttgaag gatatcaaac aatggatgca attgcagctg
    20641 ttatattctc ttcaataata ttaggttcaa taaaagctaa aggatatact ggtaaagcta
    20701 gctttaaatt acttattaaa gcagcagtta ttgcaatcgg aggactagca ataatttatg
    20761 gtggattaat attccttggt gctcatactg gggatttagc tttagattta tcaaattctc
    20821 aattattact ttttgttgct gaaagtattc taggacatgc aggtgcaata ttaataggtg
    20881 tatcaatggc attagcttgt ttaacaactt caataggatt actttctgct ggtggaagtt
    20941 tctttgaaag agtttctaat ggtaaattac catataaatt taacgtttta ttaatgacag
    21001 taactagtat attagttgct agtatgggat tagataagat aatatcatta tcagctactt
    21061 tacttggtgt aatctatcca gtaacaatag ttcttatatt attaaaccta ttccataaga
    21121 gaataactaa cccacttact cataaggtta ctgtatatgt tgctttaata gttagtatat
    21181 taataacact aggtaacttt atacctggag tagaaaactt agtttctctt cttccactat
    21241 actctaaggg atttggttgg gtagtacctg ctttaatagg atttataata ccaagcatta
    21301 tgcctacaag agaaactgct acagcttaaa atataatatc ttataatat
//
LOCUS       DAEVGI010000027        21264 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEVGI010000027 DAEVGI010000000
VERSION     DAEVGI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 21264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21264
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.027"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..730)
                     /locus_tag="K8M77_000922"
     CDS             complement(<1..730)
                     /locus_tag="K8M77_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085434.1"
                     /translation="MKNSKEALEVVYNNALDLILNKKLKEGEKLLEKIYGSGNINIDI
                     LNLLGIIKYMYCDFYEAKKYWNESLKVSCEDNKASEFIEDVESDDFKLISEKYIYSLN
                     LIEDKNYLQAIENLEEINKGRSELIEVKILLALLYMIIDEKNKAFKNIESALSYDISN
                     YKIKEIYNEIKCDLAKDEEKEESEELTFSESILNLIMILNYRINSFYEEELKYRNEEI
                     KSLYEEIKTMNLEIESLNTKKEKLS"
     gene            complement(748..2475)
                     /locus_tag="K8M77_000923"
     CDS             complement(748..2475)
                     /locus_tag="K8M77_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HBI7085435.1"
                     /translation="MGRIIGIDLGTTTSEIAYVKDGKPCIIRNIEEGDEHAIPSVVSI
                     EGDEIKVGKKAKNQILLKPELTVAEVKRVMGTENIIQIQGKEYRPEEISALILKKLKE
                     VAEYFLGEEVEEAVITVPANFNDIQRKATKNAGEIAGFKVERIINEPTAAAMAYGVDN
                     LDKNGNILVYDFGGGTFDVTILEMFNGVLDVKVSRGNNYLGGKDIDNKLIDHVVNEFN
                     KSTGVKLDTSDSRILARLKEGVEEAKKTLSTSKMAEIVLPYISADKDNNPINLEMVLT
                     REEFEFNVKEIIDSTEDIVNEALEDANITDNEIDTVLLVGGSSRIPYVRNMLEKRFKG
                     KIARGVNPDEAVALGASVQAAIKNDIPVGDSGTIIVTDTCNYTLGVALKGGIFDPIID
                     RDSKLPADVTKRYCTVMDNQTEVLVSVYEGESRYVSDNTLIDEFILSGIPKADTGKEK
                     IDITFTYDLNGILNVSAEILSTGDEISKIMSTKGLSKEEIKDLKEVVSSNENNNELVP
                     WKSANLFKLVEPNFRLAERKIPMLPSADREKLQEIMSDMKDAIVDNDKMALMKLDEEL
                     TDFLFDFVN"
     gene            complement(2463..3065)
                     /gene="grpE"
                     /locus_tag="K8M77_000924"
     CDS             complement(2463..3065)
                     /gene="grpE"
                     /locus_tag="K8M77_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBI7085436.1"
                     /translation="MNYEEALKDICEEKKYIDLFEEDRGLKEIKNSIKKIVIQNENMN
                     QGYIEEVYKIASSLEGITTYISKKSEEEELRSKRINKELEVREKRLVKSIISIVDNIK
                     YIYDFSQKSSNEVLNEAMNNLLNTVKRAMVNVDILLIDGKETFFDESLHECVSTIWND
                     ERKDYEINEVLKFGYIYKGKVERTAQVVVVKNKEDMKWEE"
     gene            complement(3083..4351)
                     /locus_tag="K8M77_000925"
     CDS             complement(3083..4351)
                     /locus_tag="K8M77_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI7085437.1"
                     /translation="MIDYFKELNIQIDASDNEVKNAYFNMTKKYPPEKFPKEYRVIRD
                     AYETLIDKSKRDAYILETFDIEIKNILNEGIDLAKSEKYDLAVLNFEKVLKKYPDNSK
                     VKKDLAVCLMRGRNYKKSSKILKELVIREPNNIEYYKLLINIYGDNYDLKNLEGVLKK
                     SLNLKNVEVDFYLKLFEIYNESELRDYTKAIKVLKDGLENKNINSKKYKLYLKFLDLS
                     DRLDCKDDFNKGCEALSGIILKDNYEEVKSSILNLLDRILKEFHFKNGVRLTSTALVL
                     IDEKKDVETLEKIINLRRSFLELSRLYEDKSINEDFKKIVFYNAVSKFLKDDIEFNKD
                     FERINQNFFNNLNFESDELVKSIGKLKNDYRNVYLETRKLSDKVLGRYSKVQKIKEEK
                     NSSKEFYSNRREGNPVKILFRKVINSFRDK"
     gene            4606..6933
                     /locus_tag="K8M77_000926"
     CDS             4606..6933
                     /locus_tag="K8M77_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI7085438.1"
                     /translation="MKHGPMLNKIKLFFIVQIILSFLVLAGTLISILVIPSHLPDYFI
                     WAIAFSFWVFQCGFSTIFAFRYRFSKSSLSTVKLSFILIGSIQILITLATNLFSIFDL
                     PIFNTLIIKNFQLLLLSLIFIVMIYKLIKDMLKYRVLKDYIIRDSFFTFIPFAFIIYK
                     NSKYFISSLYFLDDSVYSLALLNINSLRIFLEGFLIILIFVKTIYLRGNKKSSFLLLI
                     YFYISNLNCYIVSYFNSKLLFYHYLYQGIGIYFICPILLILSTYYSITEQEKTPDKVE
                     FDCSYMNINNSWISITIGIFPVSFSAIDIIASSLESGDFNGIESLVIILLVMILLVCR
                     DIILRKDNLRLIASVKRANEIDFLTNMYSRNYFLKKLEAMNTDYALFFIDIKSFKDIN
                     NIFGHVVGDKVLVEVANKFKELKLLLGKSVFYCRYSGNEFALISKLEQVDLILKFFRD
                     LENIKIQHNSDLIHINFNIGYSLNDSNINLDLLIQETDYALMRAKKLKGPNNSILMYD
                     DNLKTDLEHINILKKDFINDLLADKFHLVFQPKVCVKTLKIVGFETLLRWKHDKLGEI
                     SPCKFVPIAENLGEISSLDLYVFKKSCEFQRCLIDMGIEIKCSVNLSLNTLKSYEKIN
                     EILNIYKKYKIPKHLITAEILENVSLNNNAKTISYINLLRENGICISIDDFGTGYSSL
                     SQISNLYFDELKIPREFVINARTSSKLAVIEAISILAKKLNVTSVIEGVETYEDFKLF
                     SSLGFDIVQGYYFSKPLTKSEIIDYIKVNINNKIS"
     gene            complement(7025..7795)
                     /locus_tag="K8M77_000927"
     CDS             complement(7025..7795)
                     /locus_tag="K8M77_000927"
                     /inference="COORDINATES: protein motif:HMM:NF014566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI7085439.1"
                     /translation="MKKFFKTVGVCLGFPILNFLIVSFIQVFFLGYTKNNISLFQKNI
                     FSLTILGDILTLVLITIILLPSNERLLRRIKIKKINLKEYFYIIALSIGVSILLLFLS
                     GILSKIIPSYGDVVNQLNVASKSSLQLVIAIILIPIYEEIVFRGIIFGYLRKNFNIIV
                     AVLVQALIFGIMHLNLVQGIYTFILGIVLALIYMYSYSILGNITVHIIFNLLGALIVP
                     MLLSKFPIMVIVLLILGIVLFIFSVIKIIGKYEKSLYK"
     gene            complement(7808..8056)
                     /locus_tag="K8M77_000928"
     CDS             complement(7808..8056)
                     /locus_tag="K8M77_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085440.1"
                     /translation="MENQIKKSKAPIILGVISCIAWLIPLIGIIISIVGIVMSSKKIK
                     EFSCKAYKIALTLNIIGLVLTIIYFVINFVILYKQLLA"
     gene            8191..8511
                     /locus_tag="K8M77_000929"
     CDS             8191..8511
                     /locus_tag="K8M77_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBI7085441.1"
                     /translation="MENVKLMNKSKDLVHSLYNSLSKSKENNFDDIKEVLLKVYTKLD
                     LCDEKNIPLINRLVNYIYFTAYTKKLAFSSNEENIIRELSDIGKYAGLNGVYRSNYGD
                     KSQF"
     gene            complement(8609..9301)
                     /locus_tag="K8M77_000930"
     CDS             complement(8609..9301)
                     /locus_tag="K8M77_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBI7085442.1"
                     /translation="MKVRRRKYKTEQEVPSYWPSFVDIMTTVTLVFFFILLTSIGISS
                     IFVDNIAVERETVYDKIQGELNQYAVNEEVMNFNREEGKIDIKTETFFDSDSSVLKKD
                     GKKLASELGDIFFKLLSNEDIKEKIDYIEIVGHTDFAGSTIHGRELSTERAISFLNEM
                     MPEDSELENLHGEKFKASGMSEFENYASKEERDRGYDEYNIEETKKDRRIEVRMVFSN
                     EDLEKAIKERKN"
     gene            complement(9304..10734)
                     /locus_tag="K8M77_000931"
     CDS             complement(9304..10734)
                     /locus_tag="K8M77_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085443.1"
                     /translation="MSTISSILKSFLTQNILGYIGITLILIVICLGFIYNMRVKKKYE
                     NLLNAFNESEWINVSNNEEKKELKFLNSELKAMTDDFRRSAIKGTYNINTEVIIQKNI
                     EKKILKEENIANILPSISIALGLIGTFLGLTVAIMTTTGILSSGTQSMADFSRSMNMP
                     LGSMSSAFWTSIVGVIGSIILNYLNINLKRAKEDFYDVFEDYLDNILFSIYIKSDKDV
                     LTDFMNKALVSFSIKLDELFNKGINNLVEGINKNTVDMTSTFNGMNKHISDLENIVSY
                     FRVSVMGIKTPIKSFGEDLEKFSRVSAEFCNSVNSTNNKFLNKSTRLEESVNELSKAI
                     NDNKREINKLCMRLEVQSNEVRRGYNTFNDVFNIIKDEQSRNNKIISNQISKLNDGYR
                     EFERGINKFSSNVSNMKNEVANGIYYALQRETQELSSNIVDKLNVPLRDISLATEELS
                     RSTNRNREIGKVDSGWKKDSKEIGRC"
     gene            complement(11147..11923)
                     /locus_tag="K8M77_000932"
     CDS             complement(11147..11923)
                     /locus_tag="K8M77_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085444.1"
                     /translation="MFTKGFDEAYNRALELQKLFYDEKEKYNEELKNENRDIKHLVNK
                     INEEKIYILETLVPKYAKNMSILERQSIKATLGEEVSSEYIINDYNIRPIKELLLIDY
                     DEKSIIKNIKNPFIKVFKINKINKNLDEETMEIQVARDEMKKISQCAKDDKLKLIRIY
                     KLICSATNLGEAIEERFRDFIPMIQSSRLIYGDCNIEKRSYRIGNVMKCYDGIIQFLV
                     KICETKYRSVEGKRVKYIKDSLINEHLEELDLWKQSFNEI"
     gene            12096..12171
                     /locus_tag="K8M77_000933"
     tRNA            12096..12171
                     /locus_tag="K8M77_000933"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12129..12131,aa:Met,seq:cat)
     gene            complement(12205..13059)
                     /locus_tag="K8M77_000934"
     CDS             complement(12205..13059)
                     /locus_tag="K8M77_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBI7085445.1"
                     /translation="MKTLEYNESNKHKYLELKEKNSLDLYLGYCGMEKCLPSHSYGPA
                     VRNNYLIHYIIDGEGTYYVNGQSYRLRKNQGFLICPNTLTYYKASKDNPWTYMWIAFN
                     GIKAKKYLNYANLNEDNLIFEYTEDDSLEIYINRMFELNRMNYSNELEIQGLLYLFLS
                     KLAMSKNENSLKRNNISDFYVEKAIEFMQNNYLNPNLKITDVANFVGLNRSYLTHLFK
                     KSLKLSPQEFLLNYRINEATSLLKNTNLSVGTISKSIGYNDQLTFSKVFKKVKGISPK
                     TYRDNFLK"
     gene            13351..15543
                     /locus_tag="K8M77_000935"
     CDS             13351..15543
                     /locus_tag="K8M77_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBI7085446.1"
                     /translation="MSINYNENLKTFHLKTKNTSYILKILETGHISHLYWGRKLKADN
                     LEYFVRRRCFGSFCADTDNISGFQLELIPQECPTFGATDLRSPSLEFQYEDGTSATDL
                     RHKSHRIYEGKQRLSGLPAVYVESEEEATSLEITLVDSLKNLEVVLTYNVFENFDAIT
                     RSLKIVNNSDEKINIERVLSANVDFTTDEFDFIQLSGSWGRERHILRNPLRSGSQAIE
                     SRRGASSHAQNPFMALCSKDTNEEYGDVYGFSLVYSGNFLANVEVDMYRNARAQIGIN
                     PFDFKWLLESKEEFQAPEVVLVYSSKGLNGMSQIYHNLYRKRLCRGNYRDKVRPILIN
                     NWEATYFDFNEVKIKEIAKEASKLGMELFVLDDGWFGNRNDDKSSLGDWFVNEGKLKG
                     GLSKLAKDINNMGLKFGLWFEPEMISPISKLYEKHPNWCIHIPGRTRSQARSQLILDL
                     SRKEVCDYIIESVSKILESANISYVKWDMNRNMTEVGSLELTSERQRETAHRYILGLY
                     RVMEEITSRFPNVLFESCSGGGGRFDPGMLYYMPQTWTSDDTDAIERLKIQFGTSMVY
                     PPISMGCHVSAVPNHQANRITPLETRGVSAMAGNFGYELDITKLSEEEKEELKEQISL
                     YKEIRETVQFGTLYRLKSPFNSNEVAWMMISEDKNEVVVSYVRQWALVNESFSNLKLT
                     ALDKDSEYEIIGEDIVLSGDELMYIGLNIPELYGDYVSKLWKLKKKDL"
     gene            15884..16120
                     /locus_tag="K8M77_000936"
     CDS             15884..16120
                     /locus_tag="K8M77_000936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085447.1"
                     /translation="MIKRYITAFVYSDFYDWGFVDIYSIELTFVNETTVSLTDQVLQD
                     LCKTCFGGMGVNIITLCHKNLTLFHLIKANSNIS"
     gene            16194..17477
                     /locus_tag="K8M77_000937"
     CDS             16194..17477
                     /locus_tag="K8M77_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBI7085448.1"
                     /translation="MKRGLRNIIAMTLATFTVGSFLTACGNDNLKSGNSSDGNVILTY
                     SIWDKGQEPAMRAIADAFEKENPNIKINVEITPWDQYWMKMDAAAQGGALPDIFWMHS
                     SQISRYGRNGKLLDLTDRIKNSKKINLNNYPEGLVELYSPDNKNYAIPKDYDTTALWY
                     NKALFDEAGIPYPDETWTWDTLLETAKKLTNKENKIYGFGAPLNSHEGYYNFIFQNGG
                     YILSDDKKKCGYDDKKTLEALKWYIDLGLKEGVSPTQKEFEEVSPLTLFESGKLAMGI
                     FGSWNVANFKQNEYVRENCDIAVLPMGEKRASVYNGLGNAIGYNTKHPEEAWKFVEFL
                     GGKEANILQAEYGAAIPAYKGIDEKWVEVTKEFNARAHVEMLDYAEILPYSNRTARWK
                     VIEDDLWPRVWAGQANLDEVSKRMDKDIEEVLAED"
     gene            17522..18469
                     /locus_tag="K8M77_000938"
     CDS             17522..18469
                     /locus_tag="K8M77_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI7085449.1"
                     /translation="MNISVKSKEKLKLKRIEKEELKFNKKNMKEWGWGYVMIAPTIIG
                     LLILNIFPAIKTFILSFQKTGDFGNNVWTGLSNYKKLLTDEVVISAIGNTFKYAIIVV
                     PISIILSLIVAVLLNQNIKGKSLYRTIYFLPMVAAPAAIAMIWKWLFNSEFGIINQIL
                     NLIGINGPMWLSDSKTALISIAVVGIWSCIGYNMVLLLAGLQEIPKDYYEAANVDGAG
                     PIRTFFKITLPLLSPTMFFVVVTSVIGSLQVFDTIFMMIDKTNVAIEGTQSLVYLFYK
                     YSFVVNDQGYGSAIVMLLLAIIMVITFIQLKVQKKWVHY"
     gene            18486..19313
                     /locus_tag="K8M77_000939"
     CDS             18486..19313
                     /locus_tag="K8M77_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBI7085450.1"
                     /translation="MDKKIKTKKILIHGALICGALIMIVPFLWMVLTSLKTMGEATQV
                     PPKIFPSKPMYENYSEVSRLLPFGKFYMNTIIMLIFRVVGSVFFSAMAAYAFARLNFP
                     GKKIFFALVLFQMMIPAQIFIVPQFLLVQKLGLLNTISALVIPGIVSAFGTFLLRQFF
                     MGLPKELEEAAKLDGCNTWQTFYKVMLPLARSGLVALGIFTALFAFKDLMWPLVVNMS
                     VDKMTLSAGLASLQGQFSTNYPQLMAGSVLAIWPMLIIFVIFQRKFIEGIATSGSKL"
     gene            complement(19403..20152)
                     /gene="srtB"
                     /locus_tag="K8M77_000940"
     CDS             complement(19403..20152)
                     /gene="srtB"
                     /locus_tag="K8M77_000940"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HBI7085451.1"
                     /translation="MKNSLRIKKIISLILLIIIFFCAIKIYQKRLGYYTDSRDYDNLR
                     SLSPLSDSSINKDPEENKKDIENEKNLKNINSDYKFWINVEGTNIDFPVIQGKDNDFY
                     LHHNFNKEKSFSGSIFVDSENNLNDDSNLVVYGHNMRNDTMFAQIKHFKNENFFNANK
                     YVTLYREGKKSTFEIFSVYQENAKGLESQIKTKFSNKEDYEKYLKEQEEKSLFKREGI
                     DLKDNDRILTLVTCGYDFVNARIVIVAKEVN"
     gene            20338..21144
                     /locus_tag="K8M77_000941"
     CDS             20338..21144
                     /locus_tag="K8M77_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBI7085452.1"
                     /translation="MKNGKRVKNPKRNSSNRSKSKVLDISIIILIIALAVVIAVGTIR
                     DKKTLAKNMEIYSSNSSNNSEDNKKQATTSNPYEKIKDGKEVNILVLGNGVGLSEGKT
                     DESGNWTISLSKWLEETYKSKANIINLSQDKATVKVGLEELEKNKDNFDLAYIIFGEN
                     DQKVLKPNEFKEDYEKIVKNLKERNKDISIVPIIENSIRKKNEYTNIIEEIGKANNLK
                     VLDMATVFIKDPAPYAKLTTKSGIYPNNLGYSLYFSNAKSLIEANIAALN"
BASE COUNT         7442 a         2976 c         2701 g         8145 t
ORIGIN      
        1 aactcaattt ttcttttttc gtatttaaag attcaatttc taaattcata gttttgattt
       61 cttcatataa ggattttatt tcttcatttc tatactttaa ttcctcttca taaaaagagt
      121 ttatcctata attcaaaatc attattaaat ttagaataga ctcagaaaaa gttaattctt
      181 cactttcctc tttttcttca tcctttgcta aatcacattt tatttcatta tatatctctt
      241 ttatcttata atttgatata tcatatgata aagcactttc tatattttta aaggccttat
      301 ttttttcatc tattatcata tataataatg ctaatagaat tttaacttct attaattcac
      361 ttcttccttt attgatttcc tctagattct ctattgcttg taagtaattt ttatcttcta
      421 ttaagtttag tgaatatata tatttttcac ttattagttt aaaatcatca ctttctacat
      481 cttctataaa ttcagaagcc ttattatctt cacatgaaac tttcaaactc tcattccaat
      541 atttcttagc ttcataaaaa tcgcaataca tatattttat tattcctaaa agatttaaaa
      601 tatctatatt tatatttcca gagccataaa ttttttctaa gagcttttct ccctctttca
      661 atttcttatt taagattaaa tctaaagcat tattataaac aacttctaaa gcttctttac
      721 tatttttcat aagaaatgct cctaatatta atttacgaaa tcaaataaaa aatctgtaag
      781 ttcttcatct aatttcataa gtgccatttt atcattatct actatggcat ccttcatgtc
      841 actcataatt tcttgaagct tttctctatc agcacttggt agcataggaa tttttctttc
      901 tgctaaccta aaattaggct ctactaattt aaataaattg gctgatttcc aaggtacaag
      961 ttcattatta ttttcattac ttgaaactac ttctttaaga tccttaattt cttcttttga
     1021 aaggccttta gtagacatta ttttagaaat ttcatctcca gtagaaagaa tttctgctga
     1081 aacatttaaa attccattta aatcataagt aaaggtaata tctatttttt ctttaccagt
     1141 atcagccttt ggtattcctg ataatataaa ttcatctatt aaggtattat cactaacata
     1201 cctactttca ccttcataaa cacttaccaa cacctctgtt tgattatcca taactgtaca
     1261 atatcttttt gttacatctg caggtaattt tgaatctcta tcaattatag gatcaaaaat
     1321 tcctcccttt aatgccactc ctaaagtata gttgcatgta tcagtaacta ttattgtacc
     1381 tgaatctcca actggaatat catttttaat agcagcttgt actgaagccc ctaaagccac
     1441 agcctcatct ggatttactc cccttgctat ttttccttta aatctttttt caagcatatt
     1501 tcttacataa ggtattctac tagaaccacc aactaataat actgtatcta tctcattatc
     1561 agtaatattt gcatcctcta aagcctcatt tactatatcc tcagtagaat ctattatttc
     1621 tttaacatta aactcaaatt cttccctagt taataccatt tctaagttta ttggattatt
     1681 atctttgtca gcactaatat aaggaagtac tatttcagcc atcttagatg tggataatgt
     1741 ttttttagct tcctctaccc cttccttaag tctagccaaa attctactat ctgaggtatc
     1801 taattttaca cctgttgatt tattaaattc atttaccaca tggtctatta atttattatc
     1861 aatatctttt ccacctaaat aattatttcc tctacttacc tttacatcta aaactccatt
     1921 aaacatttct agtatagtta cgtcaaaggt tccccctcca aagtcataaa ctagaatatt
     1981 accattttta tctagattat ctactccata agccatggca gccgcagtag gttcatttat
     2041 tattctttct actttaaaac cagctatttc cccagcattt tttgtagctt ttctttgtat
     2101 atcattaaaa ttagcaggaa cagttattac ggcctcttct acttcttctc ctaaaaaata
     2161 ttcagctact tcttttaatt tttttaaaat tagcgctgaa atttcttctg gtctgtattc
     2221 tttcccttga atttgtataa tattttctgt tcccataact ctctttacct cagcaactgt
     2281 taattcaggc tttagtaaaa tttgattttt agctttcttt ccaaccttta tttcatctcc
     2341 ttctatagaa accactgatg gaatagcatg ttcatctcct tcttcaatat ttcttattat
     2401 acatggctta ccatctttta cataagctat ttctgaagtt gttgtcccta aatcaattcc
     2461 tattattctt cccatttcat atcctcctta tttttaacta ctactacttg agctgtcctc
     2521 tcaacttttc ccttgtatat gtatccaaac tttaaaacct cgtttatctc ataatctttt
     2581 ctttcgtcat tccaaatagt actaacacac tcatgtaagc tttcatcaaa aaaagtttct
     2641 ttcccatcaa taagaaggat atctacattt accattgccc tttttactgt gtttaataga
     2701 ttattcatag cctcatttaa tacttcatta ctagattttt ggctaaaatc ataaatatat
     2761 tttatattgt ccactatact tattatgctt ttaactaatc tcttttctct tacttctaat
     2821 tctttgttta ttcttttact tcttagctcc tcttcctcac tctttttact tatataggtt
     2881 gtaatccctt caaggctact agctatttta tagacctctt caatatatcc ttgattcata
     2941 ttttcatttt gtatgactat tttttttata gaatttttta tttcttttaa tcctctatcc
     3001 tcttcaaaca aatctatata ctttttctct tcacaaatat cctttaaagc ctcttcataa
     3061 ttcaaatcag taccccctta ttttatttat ctctaaaact atttattact tttctaaata
     3121 aaatttttac gggattcccc tctctcctat tagaataaaa ttccttagat gaattctttt
     3181 cctcttttat tttctgaact ttgctatatc tacccaaaac cttatcactt aactttcttg
     3241 tttctaaata cacattccta taatcatttt ttaacttccc aatagatttt actaattcat
     3301 cactttcaaa attaagatta ttaaaaaagt tttgatttat tctttcaaaa tccttattaa
     3361 attcaatatc atcctttaaa aatttgctta cagcattata aaatactatc tttttaaaat
     3421 cttcatttat actcttatcc tcataaagcc tagatagttc taaaaaacta cgccttaaat
     3481 taattatttt ttctaaggtt tccacatcct ttttttcatc tattaaaact aaagctgttg
     3541 atgtaagcct aactccgttt ttaaaatgaa actcttttaa aattctatct aacaaattta
     3601 atattgaact ttttacttct tcataattat cctttaaaat gattccgctt aaagcttcac
     3661 accccttatt aaaatcatct ttgcaatcta atctatctga taaatctaaa aacttaaggt
     3721 ataacttata cttcttagaa tttatatttt tattttctaa gccatccttt aaaaccttaa
     3781 tagcctttgt ataatccctt aattcgcttt cattgtaaat ttcaaataat tttaaataaa
     3841 aatccacttc tacatttttc aaattcaagc tctttttaag tacaccctct aaatttttaa
     3901 gatcataatt atctccataa atatttatta aaagcttata gtattctata ttgttaggtt
     3961 ctcttataac taattctttt aatatcttag aacttttttt ataatttcta cctctcatta
     4021 aacatacagc taagtctttc tttacctttg aattatcagg gtatttttta agaacttttt
     4081 caaaattcaa aactgctaaa tcatattttt cacttttagc taaatcgata ccttcattaa
     4141 gtatattttt tatttcaata tcaaaagtct ctaaaatata tgcatccctt ttagacttgt
     4201 ctattaaggt ttcataagca tcccttataa ctctatattc ctttggaaat ttttctggtg
     4261 gatatttttt agtcatatta aaataggcat ttttaacttc attatcagag gcatctatct
     4321 gaatatttaa ttctttaaaa taatcgatca tatatatcct cctaaaatta attaacgata
     4381 tatctctata ccataacttt atttcagtaa aactaataat attcattggt aagcctattt
     4441 tatttataaa acttagaata ctctagatta gaactatttt tttacccata attatattta
     4501 ttttaatttg acataaatta cttatattaa taaaatgtta atgaattatt tgataaaaat
     4561 aactaataat tcaaatatta tattaagaag ctttcggagg tttatatgaa gcatggacct
     4621 atgctcaata agataaaact tttttttata gttcaaatta tattaagctt tttagttttg
     4681 gcaggaacat taatttctat attggtaata ccatcacact tgccagacta ctttatatgg
     4741 gccatagcct tttcattttg ggtttttcaa tgtggcttta gtacaatctt tgcatttaga
     4801 tatagatttt ctaaaagttc tctaagcacc gtaaagctaa gctttatact aattggaagc
     4861 atacaaattt taataacctt agcaacaaac ctatttagta tatttgatct tccaattttc
     4921 aatacattaa taataaaaaa ctttcaactt ttattattat cattaatatt tattgtgatg
     4981 atttataaac ttataaaaga tatgcttaaa tatagggttt taaaggatta tattattcgt
     5041 gattcgtttt ttacctttat accttttgct tttattattt ataagaactc taaatacttt
     5101 atatcttcac tatatttctt agatgattca gtatatagtt tagctctatt aaacataaat
     5161 agcttaagaa tttttttaga aggatttctt attatattga tctttgtaaa aactatttat
     5221 ttaagaggaa ataaaaaaag tagctttcta ttattaatct acttttatat atcaaatctt
     5281 aactgctata tagtctcata ttttaatagt aagttgcttt tttatcatta cttatatcaa
     5341 ggtattggaa tctactttat atgtccaata ttattaatac tttccacata ttattcaatt
     5401 acagaacaag aaaaaactcc agataaagta gaatttgatt gttcttatat gaatataaat
     5461 aactcatgga taagtattac cataggtatt tttccagtat cattttcagc tatagatata
     5521 attgcatcct ctctagaaag tggtgatttt aacggcatag aaagcttagt aattatactt
     5581 cttgttatga ttttactagt gtgtagagat attatattaa gaaaagataa tctaagatta
     5641 attgcaagtg ttaagagggc taatgaaata gactttttaa ctaatatgta tagtagaaat
     5701 tattttttaa agaaacttga agccatgaat actgattatg cacttttctt tatagacata
     5761 aagagcttta aggacattaa taatattttt ggacatgttg ttggtgataa ggtattagta
     5821 gaagttgcaa ataaatttaa agaacttaaa ctacttctag gtaaaagtgt tttctactgt
     5881 agatatagtg gaaatgaatt tgccttaatc tctaaattag agcaagttga tcttatatta
     5941 aaatttttta gagaccttga aaatataaaa attcaacata actctgattt aatacatata
     6001 aactttaata ttggatattc attaaatgat tcaaatatta acttagactt actaattcag
     6061 gaaacagatt atgctctaat gagagctaag aagcttaaag gtcctaataa ctctatactt
     6121 atgtatgatg ataatttaaa aacggactta gaacacataa atatattaaa aaaggacttt
     6181 ataaatgatc ttttagcaga taagtttcat ttagttttcc aaccaaaagt ttgcgtaaaa
     6241 acattaaaaa tagtaggttt tgaaactcta cttcgttgga aacatgataa actaggtgaa
     6301 atttctcctt gtaagtttgt acctatagct gaaaatttag gtgaaatttc ttcccttgac
     6361 ttgtatgttt ttaaaaaatc ctgtgagttt caaagatgct taatagatat gggaatagaa
     6421 ataaagtgca gtgtaaattt atcattaaac actttaaaat cctatgaaaa aataaatgag
     6481 attctaaata tttataaaaa atataaaatt ccaaaacatt taataacggc agaaatatta
     6541 gaaaatgtat cactaaataa taatgctaaa acaatctctt atattaatct tcttagagaa
     6601 aatggaattt gtatatccat agatgatttt ggtacaggct attcttctct tagccaaata
     6661 tctaatctgt attttgatga attaaaaatc ccaagagagt ttgtaataaa tgcaagaacc
     6721 tctagtaaat tagctgttat tgaagcaata tccatattag caaaaaaatt aaatgtaacc
     6781 tcagttattg aaggggtaga aacttatgaa gatttcaaat tatttagtag tttaggattt
     6841 gatattgttc aaggatacta tttctctaaa cccctaacta aatctgaaat aatagattat
     6901 ataaaagtta atattaataa caaaataagc tagtagttaa ttaagttttc atatttaggc
     6961 aataagaaaa agggctatat caaaatataa taataaaaat tattttgata tctccccttt
     7021 ttctttattt atataaagat ttttcatatt ttccaattat ttttataact gaaaaaataa
     7081 aaagtacaat tcctaatatt aataaaacta taaccataat tggaaattta cttaataaca
     7141 ttggcactat taatgctccc agtaagttaa aaattatatg tacagtaata tttcctaaga
     7201 ttgaatatga atacatatag atcaaagcta acactatccc taaaataaat gtataaatac
     7261 cttgaactaa atttaaatgc ataattccaa aaattaaagc ttgtactaaa acagccacta
     7321 ttatattaaa gttttttctt aaatatccaa aaataattcc tctgaaaact atttcttcat
     7381 atataggtat taatattatg gctattacta attgtagtga agatttgctt gcaacattta
     7441 attgattaac aacatcacca tagcttggta ttattttgct taaaatacca cttaaaaata
     7501 ataatagtat acttactcct atgcttaagg caataatata gaagtattct tttaaattta
     7561 tcttttttat ttttattctt cttaataacc tttcattaga aggtaaaagt ataattgtaa
     7621 ttaatactaa agttaatata tctcctaata ttgttagtga aaaaatattt ttttgaaata
     7681 aggaaatatt attctttgta taacctaaaa aaaatacttg tataaaactt acaataagaa
     7741 aatttaaaat tggaaatcct aagcatactc ctactgtttt aaaaaacttt ttcatataca
     7801 atctcctcta agctaatagt tgcttatata atataacaaa gttaattaca aagtatataa
     7861 tagttaacac taagcctata atatttaagg ttaaggctat tttatacgcc ttacaactaa
     7921 actcttttat tttcttagaa gacataacta ttcctactat actgataatt attcctatta
     7981 atggaataag ccaagctata cagcttatta cccctaaaat tattggtgct tttgactttt
     8041 taatttgatt ctccattctt ttctcctttt ctcatttttt accttttgat attaattata
     8101 tattttaatg tattatttgt aaatatttat cgacttacat tcataaaagt actataattt
     8161 atctataatt atctttatga ggtattttaa atggaaaatg ttaaattaat gaataaatct
     8221 aaagatttag tacattcttt atataatagt ctttcaaaat caaaagaaaa taattttgat
     8281 gatattaaag aggtcttatt aaaagtttat acaaagctag atttatgtga tgagaaaaat
     8341 attcctttaa taaatagatt agttaactat atatatttta cagcttacac aaaaaaactg
     8401 gctttttcct ctaatgaaga gaatataata agagagttat ctgatatagg taaatatgct
     8461 ggtttaaatg gtgtatatcg ttcaaactat ggagataaat ctcaatttta aataaactaa
     8521 ttaataatga aatgttattc tgtaaaataa atgtatataa aaaacatcat aacaataagt
     8581 tatgatgttt tttaccctct tttgtaattt aatttttcct ttcctttatt gctttttcta
     8641 aatcttcatt gctaaatacc atacgcactt caattcttct atctttcttt gtttcttcta
     8701 tattatattc atcatacccc ctatctcttt cctctttaga agcatagttt tcaaactcag
     8761 acatccctga agccttaaac ttttctccat gaaggttttc aagttcacta tcctctggca
     8821 tcatttcatt taaaaagctt attgctcttt cagtagatag ctctcttcca tgaattgtgc
     8881 ttcctgcaaa atctgtatgt cctacaatct ctatataatc tattttttct tttatatcct
     8941 cattagatag taacttaaaa aatatatctc caagttcaga ggctaatttc tttccatctt
     9001 tctttaaaac tgaactatca ctatcaaaaa aagtttctgt ttttatatca atttttcctt
     9061 cttctctgtt aaaattcatt acctcttcat taactgcata ttgatttaat tctccttgaa
     9121 ttttgtcata tacagtttct ctttctacag ctatattatc tacaaaaata cttgaaattc
     9181 ctattgaagt aagtaagata aagaaaaaaa ctaaggtaac agtggtcatt atgtcaacaa
     9241 aggaaggcca ataagaaggt acttcttgtt ctgtcttata ctttcttctc ctaaccttca
     9301 tttttaacac ctaccaatct ctttactatc ttttttccac cctgaatcta cctttccaat
     9361 ttctctattc ctattagtac ttctacttaa ctcctcagtt gctaggctaa tatctcttag
     9421 aggaacattt aatttatcaa ctatatttga tgaaagttct tgagtttctc tttgaagagc
     9481 atagtatata ccatttgcaa cttcattttt catatttgaa acattacttg aaaatttatt
     9541 tattcctctt tcaaattctc tatatccatc atttaatttt gaaatttgat ttgaaataat
     9601 tttattattt ctgctttgct catcttttat aatattaaat acatcattaa aggtattata
     9661 ccctcttctc acctcattac tttgaacctc taatctcata caaagtttat ttatttctct
     9721 cttattatca tttatagcct ttgatagttc atttacactc tcttcaagtc tagtagattt
     9781 attaaggaat ttattatttg ttgaatttac agaattacaa aactctgcac ttaccctact
     9841 aaacttttct aaatcttccc caaagctttt tataggtgtt tttattccca taactgaaac
     9901 tctaaaatat gatacaatat tctccaaatc acttatatgt ttattcattc cattaaaggt
     9961 gcttgtcata tctacggtgt ttttatttat gccttcaact aaattattta ttcccttgtt
    10021 aaataattca tctaacttta ttgaaaaact tactaaagct ttattcataa aatcagtaag
    10081 cacatcttta tctgatttta tataaataga aaataatatg ttatctaaat agtcttcaaa
    10141 aacatcataa aaatcctctt tagctctctt taaattaatg tttagataat ttaagatgat
    10201 agaacctatt acccctacaa tacttgtcca aaaggcacta gacatacttc ctaaaggcat
    10261 attcatactt cttgagaagt cagccatgct ctgtgttcca gaggataata tacctgttgt
    10321 tgtcataata gccacagtaa gccccaaaaa tgtacctata agacctaagg ctattgaaat
    10381 agatggtagt atatttgcaa tattttcttc ttttaatatt ttcttttcta tattcttttg
    10441 aattattact tctgtattaa tattatatgt tcctttaata gcactccttc taaaatcatc
    10501 tgtcattgct tttaactctg aattaagaaa tttaagttcc tttttctctt cattatttga
    10561 aacattaatc cactcactct cattaaaagc atttaaaaga ttttcatact ttttcttaac
    10621 cctcatattg tagataaatc ctaggcatat aactattaaa attaatgtaa tcccaatata
    10681 ccctagaata ttttgagtta aaaaagattt taatatgcta cttattgtac tcattataaa
    10741 aaattctcct cccctattaa ttaatcttta tatgaaatat atgattacaa ataggttttt
    10801 ataatgaatt tccataataa ataaaataaa aaagccttat gaatactcaa gctacccttg
    10861 atttcataag acttaatttt aaatatttct taaactataa actatctata taaagaataa
    10921 aggctgaaga aattattctc caaccaaatc caaaataaat actaacacgc tataagtatt
    10981 tactatatgt agacagtatc atattctggt taaaaattca ataaaatcta catatttttc
    11041 tctattcttt aaaaattttt atcattttat tgaataaact ggctgtaaca ccataaaaaa
    11101 tcataaaaat ctgcctattt ctcttgtctt aactttataa ataaatttat atttcattaa
    11161 agctctgttt ccataaatct aattcttcta aatgctcatt aattaaagaa tcttttatgt
    11221 acttaactcg ctttccctct acacttcgat atttagtttc acatatcttt actaaaaact
    11281 gtattattcc atcataacat ttcataacat ttcctattct atagctacgc ttctctatat
    11341 tacaatcacc atatattagt ctgctgcttt gtatcatagg tatgaaatct ctaaaccgtt
    11401 cttctattgc ttcccctaaa ttagttgcag aacatattaa tttataaatt cttattaatt
    11461 taagcttatc atccttagca cactgtgata tctttttcat ttcatctctt gcaacttgta
    11521 tttccatagt ttcttcatct aaatttttat ttattttgtt tatcttaaag accttaataa
    11581 aaggattctt tatatttttt ataatagact tttcatcata atctatcagt agcagttctt
    11641 taataggtcg tatattataa tcatttatta tatattcact actgacttct tctcctaaag
    11701 tagcttttat actttgtctt tctaaaatac tcatattctt agcatattta ggtactagtg
    11761 tttctaatat atatattttt tcttcgttta ttttgttaac taaatgcttt atgtccctgt
    11821 tttcattctt taactcttca ttatattttt ctttttcatc ataaaataat ttctgaagtt
    11881 ctaaagccct attatatgcc tcgtcaaaac ctttggtaaa catttctcct cctgaataaa
    11941 aaattaaaac taatatacgt ccctatattt attatatatc acaaaagaga aagttctaag
    12001 tacttgtaac aatttaaata taataaatat ataatttcct tgactttaat tatatattaa
    12061 cgtatagtta aagagtagta gatacaattt aatgacgcgg gatggagcag ctggtagctc
    12121 gtcgggctca taacccgaag gtcgtaggtt caagtcctgc tcccgcaacc acagaaagtt
    12181 ctactttttt tagtagaact ttttttactt taaaaaatta tctctatacg tttttggact
    12241 aataccctta acttttttaa atacttttga aaaagttaat tggtcattat aacctataga
    12301 tttagatatt gttccaacag agaggtttgt gttttttaat aaagaagtgg cttcattaat
    12361 tctatagttt aataaaaatt cttgaggtga gagttttaat gattttttaa aaagatgagt
    12421 cagatagctt ctattaagac caacaaaatt agcaacatcg gttattttta aatttggatt
    12481 caaataatta ttttgcataa attcaattgc tttttcaaca taaaaatctg atatgttatt
    12541 cctttttaag gagttttcat ttttactcat agccaattta gagagaaaaa gatataatag
    12601 tccttggatt tcaagttcat ttgaatagtt cattctattt aattcaaaca ttctatttat
    12661 ataaatttct agagaatcat cctctgtata ttcaaaaata agattatctt catttaaatt
    12721 agcataattt aagtattttt ttgctttaat accattaaaa gcaatccaca tatatgtcca
    12781 aggattatct ttacttgctt tataataagt aagtgtattg gggcatataa ggaacccttg
    12841 attttttcta agcctatatg attggccatt tacatagtag gtaccttctc catctattat
    12901 gtaatgaatt aaataattat ttctaacagc gggtccatag gaatgactag gaagacattt
    12961 ttccatgcca caatatccca aatataaatc taaagaattc ttttctttaa gctcaagata
    13021 tttatgttta ttagactcat tatattcaag agttttcaat ttatcacatc ctaacttatc
    13081 taacattaat ccatatataa atcacattta tatatttaat tttaatacaa ttccttatat
    13141 aattctatta aagaataaat tagagataga ctattggtaa ttttgattta gcttttcata
    13201 ataagtttag tttaaagtaa aattaatcct tttcatttca tttataatgt taaggttttc
    13261 ttttaaggtt aaaatccatt tgaaaaagtc tatataaaaa tattagtatt aatatagggg
    13321 gaagagttaa taactaactc taggagaatc atgagtataa attataatga aaatttaaaa
    13381 acttttcatc tgaaaacaaa aaatacaagt tatattttga aaatattaga aacaggacat
    13441 ataagtcatt tatattgggg aagaaagtta aaagctgata acttagagta ttttgttaga
    13501 agaagatgtt ttggaagttt ttgtgctgat actgataata taagtggatt tcagttagag
    13561 ttaatacctc aggagtgtcc aacctttgga gctactgatt taagaagtcc aagtttagag
    13621 tttcaatatg aagatggtac atcagctact gatttaagac ataagtcaca tagaatttac
    13681 gaaggaaagc aaagactttc aggtttacca gctgtgtatg ttgaaagtga ggaagaggct
    13741 acttctctag aaataacttt agttgattct ttaaaaaact tagaggttgt cttaacatat
    13801 aatgtttttg aaaactttga tgctattaca agaagtttaa agatagtgaa taatagtgat
    13861 gaaaagataa acatagagag agttttaagt gctaatgtag attttacaac tgatgaattt
    13921 gattttattc agctctcagg atcttgggga agagagagac atattcttag aaatccttta
    13981 agaagtggaa gtcaagctat tgaaagtaga agaggggcaa gtagccatgc tcaaaatcca
    14041 tttatggccc tatgtagtaa ggataccaat gaagaatatg gagatgttta tggctttagc
    14101 ttagtttata gtggaaactt tttagctaat gtggaagttg atatgtatag aaatgcaaga
    14161 gctcaaatag gaataaatcc ttttgatttt aaatggttac ttgagtcaaa agaagagttt
    14221 caagcaccag aggtagtttt agtttattcc tcaaagggac taaatggcat gtctcaaatt
    14281 tatcataatc tttatagaaa gagattgtgt agaggaaatt atagagataa ggtaagacct
    14341 atacttataa ataactggga agccacatac tttgacttta atgaggttaa gataaaggaa
    14401 atagctaagg aagcttcaaa gttaggaatg gaactttttg ttcttgatga tggatggttt
    14461 ggaaatagaa atgatgataa aagttcctta ggagattggt ttgttaatga ggggaaatta
    14521 aagggtggac ttagtaaact agctaaggac ataaacaata tgggtttaaa gtttggatta
    14581 tggtttgagc ctgagatgat ttcacctatt agtaaacttt atgaaaaaca tccaaattgg
    14641 tgtattcata ttccaggaag aactagatca caggcaagaa gtcagttaat attagaccta
    14701 tcaaggaaag aggtatgtga ttatataata gaatctgtta gcaaaattct tgaaagtgct
    14761 aatatatctt atgttaagtg ggatatgaat aggaatatga cagaggtagg ttctttagaa
    14821 ttgacttcag agagacaaag agaaacagct catagatata ttttgggatt atatagggtt
    14881 atggaggaaa taacaagtag atttcctaat gtattatttg aaagttgctc aggtggtggt
    14941 ggaagatttg acccaggaat gctttattat atgcctcaaa cttggacaag tgatgataca
    15001 gatgccatag aaagattaaa aatacagttt ggaacctcta tggtttatcc tccaatttcc
    15061 atgggatgcc atgtttcagc agttcctaat catcaagcta atagaataac cccacttgaa
    15121 actagggggg tatctgccat ggctggaaac tttggatatg agcttgatat tactaagtta
    15181 agtgaggaag aaaaggaaga attaaaggaa caaataagtt tgtataaaga aattagagaa
    15241 actgtacaat ttggaacctt atatagatta aagagtccat ttaatagtaa tgaagtagca
    15301 tggatgatga tttcagaaga taagaatgag gttgttgtaa gctatgttag acaatgggct
    15361 ttagtaaatg aaagctttag caatttaaaa cttacagctt tagataagga ttcagagtat
    15421 gaaataatag gagaagatat agttcttagt ggagatgagc ttatgtatat aggtttaaat
    15481 attccagaac tttatggaga ttatgtttca aaactttgga agttaaagaa aaaagattta
    15541 taataaggat ttgtttaagc gttaatgtgt tcagttatat acttacaaat aacttaaagt
    15601 taaaagcaaa acttcaagtt tatcttttta atttatatct ttgaataaaa aagtgctgtt
    15661 tatctttaag tagagaatta atatggttat atataacttg aaacatatta aaaacaaact
    15721 taaaatatag tatagaataa tagttcataa ttaacttttt tatatttttt tataatatat
    15781 aggagtttaa ttatgaactt ttttaagtca taattaacta aaggaaagat aaaattacta
    15841 atagagataa ggttacatag tcatgaaaaa gatatattaa aatatgatta agcgttacat
    15901 aacagctttt gtttatagtg atttttatga ttggggtttt gttgatatat actctataga
    15961 gttgacattt gtaaatgaaa ctacagtttc tttaacggat caagttttac aggatttatg
    16021 taaaacttgt tttggaggga tgggtgtaaa cattatcaca ttgtgccata aaaaccttac
    16081 attattccat ttaattaaag ctaatagtaa tatatcataa agaagtaata acatatatag
    16141 ccatattttg ttttatttgg ttgtaaaatt aaacttgatt aggaggaaat agggtgaaaa
    16201 ggggattgag aaatattatt gctatgacat tagcaacatt cacagtagga agttttttaa
    16261 ctgcatgtgg aaatgataat ttaaagagtg ggaatagtag tgatggaaat gttattttaa
    16321 catatagtat ttgggataaa gggcaagagc cagctatgag agctattgct gatgcttttg
    16381 aaaaagagaa tcctaatata aaaataaatg ttgaaataac tccatgggat caatattgga
    16441 tgaaaatgga tgcagctgct caggggggag ctttgccaga tattttttgg atgcattcta
    16501 gccaaataag tagatatggc aggaatggga agcttttaga tttaacagat agaataaaga
    16561 atagtaaaaa aattaattta aataattatc cagaaggatt agtagaatta tattcaccag
    16621 acaataaaaa ctatgctatt cctaaggatt atgatacaac agcactttgg tataataagg
    16681 ctttgtttga tgaggcagga attccttatc cagatgaaac ttggacttgg gatactttat
    16741 tagaaacggc aaaaaaactt actaataagg aaaataagat atatggattt ggagcaccat
    16801 taaatagtca tgagggttat tacaacttca tatttcaaaa tggtggatat atattatctg
    16861 atgataagaa aaaatgtgga tatgatgata aaaaaacttt agaagcactt aaatggtata
    16921 ttgatttagg acttaaggaa ggagtatcac caactcaaaa ggaatttgag gaggtttctc
    16981 ctttaacttt atttgaatca ggaaaattag caatgggaat ttttggttca tggaatgtag
    17041 caaattttaa gcaaaatgaa tatgtaagag aaaattgtga tattgcagtt cttccaatgg
    17101 gagaaaagag agctagtgtt tataatggat taggaaatgc tattggctat aatactaagc
    17161 atcctgaaga agcatggaaa tttgttgaat ttctaggtgg aaaagaagct aatattttac
    17221 aagctgagta tggagcagca ataccagctt ataaaggaat tgatgagaaa tgggttgaag
    17281 taactaagga atttaatgct agggcacatg ttgagatgtt agattatgca gagatattac
    17341 cttattccaa tagaactgct agatggaaag taattgagga tgatctttgg ccaagagttt
    17401 gggctggaca agcaaattta gatgaggttt ctaagagaat ggacaaagat atagaagaag
    17461 ttttggctga ggattaaaaa tatatttaat cacttaatta aattagaggg agtaaatgat
    17521 tatgaatata tcagttaaaa gtaaggaaaa attaaaattg aaacgtatag agaaggaaga
    17581 attgaagttt aataagaaga atatgaagga gtggggttgg ggatatgtta tgatagctcc
    17641 aacaataatt ggactattaa tacttaatat ttttcctgca ataaaaactt ttattttgag
    17701 ttttcaaaag actggagact ttggaaataa cgtttggaca ggtttaagta attataaaaa
    17761 attattaaca gatgaagtgg tgattagcgc aataggaaat acctttaaat atgctattat
    17821 agttgttcct ataagtatta tcttatcttt aatagtagcg gttttattga atcagaatat
    17881 taaagggaaa tcactttata gaacaatata ttttcttcca atggtagcag cgccagcggc
    17941 aatagctatg atatggaagt ggctttttaa ttctgagttt ggaataataa atcaaatttt
    18001 aaatttaata gggattaatg gtcctatgtg gttatctgat tctaaaacgg ccttaatttc
    18061 tattgctgta gtaggtatat ggagctgtat aggatataac atggtacttt tattagcagg
    18121 tcttcaagaa attccaaaag attactatga agcagcaaat gttgatgggg ctggtccgat
    18181 aagaacattt tttaagatta cattacctct tttatcgcca acgatgtttt tcgtagttgt
    18241 aacaagtgta ataggttcac ttcaagtttt tgatactata tttatgatga tagataaaac
    18301 taatgtagca atagaaggaa ctcaatcttt agtttatctt ttctataaat attcttttgt
    18361 tgttaatgat caaggatatg gatcagctat tgttatgctt ttattagcaa ttataatggt
    18421 tattactttt attcaattaa aggtacaaaa gaaatgggtg cattattaga aaggagttta
    18481 gaattatgga taaaaaaatt aaaactaaga agatattaat acatggagca cttatatgtg
    18541 gagcgttaat aatgatagta ccctttttat ggatggtttt aacttcatta aaaactatgg
    18601 gagaggcaac acaggttcca ccaaaaattt tcccaagtaa acctatgtat gaaaattatt
    18661 cagaagtttc aagattatta ccttttggta agttttatat gaatacaata attatgctta
    18721 tttttagagt tgtaggatct gttttcttta gtgctatggc tgcatatgct tttgctaggt
    18781 taaattttcc tgggaagaaa atattttttg cattagtttt atttcaaatg atgattccag
    18841 cgcaaatttt tatagttcct caatttttac tagttcaaaa gttaggatta ttaaatacta
    18901 tttcagcttt agtcattcca ggtatagtaa gtgcttttgg aactttcctc ttaagacaat
    18961 tttttatggg acttcctaag gagttagaag aagcggctaa gttagatggg tgtaatacat
    19021 ggcaaacttt ttataaagtt atgttgcctt tagcaagatc tggattagtt gccttaggta
    19081 tatttacagc gttatttgca ttcaaagatc ttatgtggcc attagttgtt aatatgtctg
    19141 ttgataaaat gacattatca gctggattag catcattaca agggcaattt tcaactaatt
    19201 atcctcagct tatggctggt tcagtacttg ctatttggcc tatgttaatt atatttgtaa
    19261 tattccaaag aaaattcata gaaggaatag caacatcagg ctcaaaatta taaataaaag
    19321 gccctgtttt atttgggtgt tgattttcga atttaagtaa acgccattta aaacagagcc
    19381 taattatatt tataaacttt tcttaattaa cttcttttgc aacaattact attctcgcgt
    19441 taacaaaatc atatccacag gttactaaag ttaagattct atcattatcc tttaaatcta
    19501 ttccttctct tttaaatagt gatttttctt cttgttcttt taaatatttt tcataatcct
    19561 ctttatttga aaattttgtt tttatttgag attctaagcc cttagcattt tcttggtaaa
    19621 ctgaaaaaat ttcaaatgtt gatttttttc cttctctata aagagttaca tatttatttg
    19681 cattgaagaa attttcattt ttaaaatgtt taatttgagc aaacatagta tcatttctca
    19741 tgttatgacc ataaacaact agattagaat catcatttaa attattttct gagtcaacga
    19801 aaattgaacc tgagaagctt ttttctttgt taaagttatg atgcaagtag aaatcattat
    19861 cttttccttg aatcactgga aaatctatat tagttccttc aacatttatc caaaatttat
    19921 agtctgaatt tatatttttt aaattttttt cattctctat atctttcttg ttttcctctg
    19981 gatctttgtt aatagaagaa tcagatagag gagaaagact tcttaagtta tcataatctc
    20041 ttgaatcagt ataatatcct aaacgttttt gatatatttt aattgcacaa aagaaaatta
    20101 taattaatag aattaaactt attatttttt taattcttaa ggaatttttc ataatgctca
    20161 cctctaaata atgaattaaa tttatctata aaactacgat agtttcaata ttacaccaat
    20221 ttattagtta atacaacaat agattttttt tattttatac tgtaaaaaag accaattttt
    20281 atgtaaaata tatattatat aacatttatc tcacaaataa aaatatggag gaaaaatatg
    20341 aagaatggaa aaagagttaa aaatcctaaa agaaattctt ctaacagaag taaatctaag
    20401 gttctagata tttcaataat aattttaata atagctttag ccgtagttat agctgttgga
    20461 actataagag ataagaaaac tttagctaaa aatatggaaa tttatagctc aaattcaagt
    20521 aataactctg aagataataa aaaacaagcg accacatcaa atccttatga aaaaataaaa
    20581 gatggtaaag aagttaatat attagtttta ggtaatggtg tagggcttag tgaaggaaaa
    20641 acagatgaaa gtggtaattg gacaattagt ctttctaagt ggctagaaga aacatataaa
    20701 tcaaaggcta atataattaa tttatcccaa gataaagcta cagttaaagt aggtttagag
    20761 gaattagaga aaaataaaga taattttgat ttagcttaca taatatttgg tgaaaatgat
    20821 caaaaagtac ttaaacctaa tgaattcaaa gaagactatg aaaaaatagt taagaattta
    20881 aaggaaagaa ataaggatat ttctatagtt cctataatag agaattctat tagaaagaaa
    20941 aatgaataca caaatataat tgaggagatt ggaaaagcta ataatctaaa ggttttagat
    21001 atggctacag tatttataaa agatccagcc ccatatgcta aattaacaac taagagtgga
    21061 atatatccaa ataatttagg gtatagctta tatttttcta atgcaaaatc tttaatagaa
    21121 gctaatatag cagctttaaa ttaatataat ttatatttta gaaaagatta agtttagagg
    21181 gaaatttaga aaagtaaaat attttattat taagtaattt ataatatcac atttatagta
    21241 tcacatttat agtatattaa ttat
//
LOCUS       DAEVGI010000028        20942 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEVGI010000028 DAEVGI010000000
VERSION     DAEVGI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.028"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            127..984
                     /locus_tag="K8M77_000942"
     CDS             127..984
                     /locus_tag="K8M77_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085453.1"
                     /translation="MGRTNISYGAKNILYKLMGQLYKGKPLEILGVNDMPKIMDRLPR
                     NFFENEVLEGGPNEIFILEDGSILLFGYEDEKNIDENMFIYLEVAVKVIKRYYKHRGT
                     FPKVRMAIIYNSSVKEARNTLSFGDILICSESIFMNKLNAEKALKELKEKVLEDNLTQ
                     DDKIKFIMLPLMKSEHDDSNILRDCVQLAKSIKSERDEAIILNGIIESTDKFISEEEA
                     RELKELLEMTKIERLYNEERDLAVKNARKEEQLRIAKSLAGILSPELISEKLGITIEE
                     IYELQHAEG"
     gene            1189..1374
                     /locus_tag="K8M77_000943"
     CDS             1189..1374
                     /locus_tag="K8M77_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085454.1"
                     /translation="MELLVYVKGRRDPFTYSGDRIDVLDFEMNGIKYKQIRYFRKGFS
                     KSELIESELITRMREIK"
     gene            1621..2625
                     /locus_tag="K8M77_000944"
     CDS             1621..2625
                     /locus_tag="K8M77_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI7085455.1"
                     /translation="MKKLNKFLSLGLMVLFSLSILVGCNTSKKEEAKAPEEKTSIEIV
                     VPDGLPAISIVKMIKEKPEIMKNLDINYSIVKGSDALVSKVLKGEGDICIVPSNVAAI
                     AYNKEAKYKLAGTVGFGSLYVISSDNSVNSLEDLKGKDVYNVGQGLTPDLIFKILLQN
                     DGINPEKDLTLSYVNAASELAPLFIEGKAKYAVVPEPMLTQIMTKKPETKIVASLNEQ
                     WKKMSDSKMGYPQSSIIVKEDLAKNNSEAVQKVLKEIDNSTKWANENKEEAGAFAEEV
                     GITGKKEIIAKSLERANLNYVSALDSESEYINFYDKIYSLEPKAIGGKKVNEEIFLQK
                     "
     gene            2600..3355
                     /locus_tag="K8M77_000945"
     CDS             2600..3355
                     /locus_tag="K8M77_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI7085456.1"
                     /translation="MKKFSYKNKKLIFLSSVTLFILWQLASMIIGSEVILPSPYLTFN
                     GLIEVIKSSDFFKIIINTLGRTFISFTLSLVIALFLGVFSAFNKYVYNFMIPILNVMR
                     SFPTIGFIILALIWLSQGIAPILVGFVMSFPIFYDAIIGAILNIDKNILEMAKVYELN
                     IGNLIKNIYLPSISFAICRIMISTFSLTMKLVVGGEVIGQPKYGIGTALFVEKNYLNT
                     NMVFAWIIVVIIIGIIFSLIQKTIDGRVFRWMK"
     gene            3357..4043
                     /locus_tag="K8M77_000946"
     CDS             3357..4043
                     /locus_tag="K8M77_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085457.1"
                     /translation="MSIKLVNIEKNFGSKKIYDKFSLNFEEGKINCILGKSGCGKSTL
                     LNIIANLEEINSGEIIGIPKKIAYVFQEDRLIEWNSIYTNMELPLLKFYTKDEREEKI
                     KNILREVELGDCMNSYPKELSGGMRQRANIARALLYNGELLLMDEPFKSLDKSSKENI
                     IKIFKKNHLEKNNTVLMVTHDINEALSLGDNIFILGGNPVSLIDKFKDVQKSKEKNNI
                     EEKILLILKE"
     gene            4064..5386
                     /locus_tag="K8M77_000947"
     CDS             4064..5386
                     /locus_tag="K8M77_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7085458.1"
                     /translation="MSKKLNLLQDKESTLLRKYMIPSVLGMLGLSVCILFDTMFIGHK
                     IGDLGLAALNIALPIYNLYSAIGLTIGVGGATALSVAMGQKKFHRVNRIFTSAVFATI
                     IFCIIISLLEVFFLDKIVYMLGASEATFPLAKEYLKIILAFNPAFIFASAFIVFVRND
                     REPKLAMYAVIGSNTTNIVLDYVFIYIFNLGMFGAALATSIGQLVALGVLSIHFIKKN
                     NTMHIELGGINLDNIGKVLKNGVPSFLNEISAGFVIFIFNIVIYKFEGDLGVSAYGII
                     TNIVLIFMAVFNGVSQGVQPLISVNFGAKKEERVKEFLRLTRIIVLILGVVFLGMGLL
                     LPNQMIGLFTSDRGRLLSITRQGIYLYFIAFLFNGSNILNIAYLQAVEKSKESSLLST
                     LRGLVFVIIFIIVLPRIIGVYGVWLTIPVTELSTLLLFSIFEIKKKKI"
     gene            5620..5814
                     /locus_tag="K8M77_000948"
     CDS             5620..5814
                     /locus_tag="K8M77_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HBI7085459.1"
                     /translation="MDIGNYREMDPYMLLSIINLKLRDYYSDIESLCDDLEIEKEIIG
                     QRLKDCGYIYNRENNQFVAE"
     gene            5855..6820
                     /locus_tag="K8M77_000949"
     CDS             5855..6820
                     /locus_tag="K8M77_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBI7085460.1"
                     /translation="MNLRKEPYLLVFGASVVDVFGFSNASYRAYNSTPGHVKISFGGV
                     CRNIAENMARVGVNTNFMSILGNDEHGKSIVEHSKKIGYHMDDSMVIEGGSTPTYLAI
                     LDENGEMVSAIADMKSIGAMNTDFIDSKREIFENAEYTVLDSDNPEIMEYLLKNFKDK
                     TNFILDPVSAEKANWVKHLIKDFHTIKPNRHEAEILAGFPITDIDDLIKASNYFLGLG
                     IKKVFISLDADGIFYNDGVSCGKIKATEVDVKNVTGAGDSFVAGLGYGYMNKMPIEDI
                     VKFAMTMSNITISHEETIHPDMALDTVLAKLEKTAWVEEKYDLNK"
     gene            6897..7049
                     /locus_tag="K8M77_000950"
     CDS             6897..7049
                     /locus_tag="K8M77_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085461.1"
                     /translation="MSKKIKKGKLALLALAGSAFVASLLYDKNQKNKKDMENLELELQ
                     NIEAKE"
     gene            complement(7088..8743)
                     /gene="sulP"
                     /locus_tag="K8M77_000951"
     CDS             complement(7088..8743)
                     /gene="sulP"
                     /locus_tag="K8M77_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HBI7085462.1"
                     /translation="MYKPKLISLLDDKESGFSKEQFLKDLIAGIIVAIIALPLSIALG
                     ISSGVSPEKGLITAIIAGFIISLLGGSRVQIGGPTGAFVVIVFGIIQNHGVDGLIIAT
                     FMAGIILVLFGLLRFGSLIKYIPYPITVGFTSGIAITLLSTQVKDFLGLSITKTPSEF
                     IPKWEAYISHMNTTNLYTLAIGLLALIILIFWPKINKKIPGSLIALIVTTLVVFIFNL
                     PVATIGSQFGKISSNIPIPHIPNLNLNTLKALIGPAFTIALLGGIESLLSAVVSDGMI
                     GDKHNSNAELIAQGLANIGSSLFGGIPATGAIARTAANVKNGGRTPISGIVHSITLLL
                     IMLVFMPLAKFIPLTTLSAILIIVSYNMSEWRTFKAILKAPKSDIAILLITFFLTVLF
                     DLVIAIVIGMIVSMCLFIRRVATSIEVNELNESDCSYKSNIDTDMENLKVGENVLVYD
                     IRGHLFFGAVDTFMNTMKEINDDAKVLVLRMRHTKTLDVTGYKQIKNIALSCKSRNMT
                     LIISELQEQPKKVMRLMGFIYTLGEDHFATNFDEALEKANSLI"
     gene            8963..9598
                     /locus_tag="K8M77_000952"
     CDS             8963..9598
                     /locus_tag="K8M77_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085463.1"
                     /translation="MSLFLGKIHFWLFDKIKWFENLEEEVLKIAKERNMPVEEWISYA
                     NLNFGEKTPNKPLDEIIDESNIHGWLEGRINSAESRCAYYITNMLKEDSEVKKELIEL
                     YENHGKINADECKGQIDGENILEVYNSLNDYILDGMPCDRINEVLENSPEKIVWHMSR
                     DLHERFWESVGGDVNEFHDLRNSWIKAFVEEINPQLELVIYENGHKAIVRK"
     gene            9717..10253
                     /locus_tag="K8M77_000953"
     CDS             9717..10253
                     /locus_tag="K8M77_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085464.1"
                     /translation="MYLAQGAIISLDLGKGHIIDKDTSDDLKEKIQRTILYFFKKEVD
                     KNNLKFIDFTPYNKFFISNGEKLKSIVKRVNRSESDLHITLNINFSKSNEIFCFVEEF
                     DSKGKKFAENICSFFELINYKNKGIKESDVYNLLYIDKPSIIIDLNLNINDESEVDEK
                     GECIAKTLVESILSLGTK"
     gene            complement(10270..10986)
                     /locus_tag="K8M77_000954"
     CDS             complement(10270..10986)
                     /locus_tag="K8M77_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBI7085465.1"
                     /translation="MKKFVFLLITLIFSFSALATSSKPIKAAELEESTKKIFLTFDDG
                     PGGKVTEEILNTLKENDVKATFFLIGELVEKHPDLVKRMNDEGHSIGVHTFTHERNKI
                     YRNDSSFLEENLKTQGSIEKVIGKKVFSLRFPFGSNNSTYTLKKSLVDSLHEQGFRIY
                     DWTVDSTDALNPNLSPSSIVQKSISNSDYIVLLMHCGYSNNNSAKALPSIIKHFKSAG
                     YSFEVIDESTPEIYRLKNKC"
     gene            11162..11446
                     /locus_tag="K8M77_000955"
     CDS             11162..11446
                     /locus_tag="K8M77_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085466.1"
                     /translation="MEKYLRILLGITVTILIFAFAIWILPYVLIGVAILILIGWVYFK
                     FIRKYVNKNKKEKTETYTYTFTKEEKKSEADMQDDGPVIDVDFEEVKDDK"
     gene            11458..12342
                     /locus_tag="K8M77_000956"
     CDS             11458..12342
                     /locus_tag="K8M77_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI7085467.1"
                     /translation="MNFRKLKIFFETAKCLNMTKVAKSMYISQPSISQAIAELESDLD
                     VKLFDRIGKRLYLTHDGEVYFEYSRRILNLYEEANSIIRSSKEGQKGKIIIGASTTIG
                     IYILPKLIKEFNELHKNIEISLIIENTQLIEELIIENKVDIALVEGYVKSNELEVFDI
                     GKDELVFISNPNNPIFLKDKITLKDLENENFIMRESGSGTREIIENYLIKQGCNYNVY
                     MELGNTEAIVKVVETGLGIACVSCKAIEKRINDGLIKEIKIDDIKVNRDLYLIYHKDK
                     FISRNLKIFIDKIKNSDI"
     gene            complement(12394..13041)
                     /locus_tag="K8M77_000957"
     CDS             complement(12394..13041)
                     /locus_tag="K8M77_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBI7085468.1"
                     /translation="MKNMFFREPVNGFTHLFGAILSIFALVALTAKATYIYGIGLPLL
                     SVILFGFSLVLLYSASAIYHLVNAKEKTIAFLRRLDHSMIFILICGSYMPYCLIGLEG
                     KAQIFITSLIVILTILGILFKMVWFNCPRILSTLMYIGMGWISLGAIPFFKNIPALAL
                     IWLAIGGVIYTIGGIIYALKPKFLEFNNLGFHEIFHIFILLGSLCHFISIFFYLL"
     gene            13279..13833
                     /locus_tag="K8M77_000958"
     CDS             13279..13833
                     /locus_tag="K8M77_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBI7085469.1"
                     /translation="MNLNDLLESLNLDNNIKLEDIPELDLYMDQVIQLFENKLGGNKR
                     FKEDKILTKTMINNYAKGNLLMDIKNKKYSKEHIILMCFIYNMKGILALSDIKLALGD
                     IVEGYNKEEFNSEDIRKFYEIYLKGQKKNVKDIKSEINIDLNNLGEEFNSLESSEKKL
                     LLSAILVDKANIYKKLAEKILDSK"
     gene            13915..14562
                     /locus_tag="K8M77_000959"
     CDS             13915..14562
                     /locus_tag="K8M77_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085470.1"
                     /translation="MDYKDCQCNSRGIELNELEELFEDVRCNNYYNSCDNYHKYDHCD
                     HHNHCDHHHEYCDHHDNCDHHHDNCNHHDHNHGCCDDRRDCCKRPQPKPIPKKGTIEV
                     QSLLGCSDGEPISGMPIELYKVDCNTGFELVDCKYTDRCGKVCFKCLEDGLYAVKQPV
                     DRCYFEAPEYYPCYEVCISPRNKYVKVVIINKLRDMEDCNRPPIHCRCNSEASCQ"
     gene            14697..15161
                     /locus_tag="K8M77_000960"
     CDS             14697..15161
                     /locus_tag="K8M77_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyuridine 5'-triphosphate
                     nucleotidohydrolase"
                     /protein_id="HBI7085471.1"
                     /translation="MIIRIKYFDNATKLKKITKGNWIDIYANKDIFVKCGERAMVPLG
                     FALELPEGWEGHLAPRSSTFKTWGIIQTNSVGVVDDTYIGDNDQWHMPVYCLQGKDIE
                     SENGEEVKGTWIRKGDKIGQFRIMEVMPEIEFEEVESFGNKDRGGFGTTGTK"
     gene            complement(15326..17257)
                     /locus_tag="K8M77_000961"
     CDS             complement(15326..17257)
                     /locus_tag="K8M77_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBI7085472.1"
                     /translation="MIILSCRHLTKSFGIDEILRDVTFNINEGDKVGLIGPNGEGKST
                     LFKILTKQLDYDSGELFLDKNKTLGYLSQNLSLDSENTIYDEMLSVFSSLTELEDKIK
                     TLEEKLNEPYDASKEEYHNKLIKDYTLSQELYENRGGYTFRGEISRVLKGLGFLEEDF
                     NNQIINLSGGQKTRLALCKLLLRKPDLLLLDEPTNHLDLEAIEWLEEYLNGYKGTVFV
                     ISHDRFFLDSVTNTTFELIGGKIHCYNASYTKFLELRKKDIEARLKAYNLQQAEIKRQ
                     EEIIEKFRSFNREKSVRAAESRQKALDKMDRLEAPEVIKEASKISFETLVKSGNDVLH
                     IENLKKSYDSLLFENVNLDLKRGEKVALIGENGRGKTTLFKIIMGMLKQDSGSVSLGK
                     NVFTGYYDQEQSNLNLHKTIIDEVWDDFPNLTTTEIRNYLASFLFTGDDVFKEISLLS
                     GGEKCKINLLKLMLSKANLLLLDEPTNHLDIMSREALEDAILSYDGTLIVISHDRYFL
                     NKVINKIVELQENGLKTYLGNYNYYMDKKANPNRYEDIDEEINQGKTKTQIKEERKKK
                     KSAEKSARALRAQLRDVEKLIPQKEEELEKLQGMLCAEEVYSNPEESVRVNKEINSVQ
                     EEIDSLYATWEELSESLEE"
     gene            17427..18713
                     /locus_tag="K8M77_000962"
     CDS             17427..18713
                     /locus_tag="K8M77_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBI7085473.1"
                     /translation="MNNNFRELFIGVDEEITIYNGKKIVPINLDNAATTPALKEGLKC
                     VSDMLHVYSSIGRGKGYKANFCNEFYEKSRKKILEFFNVKNTEDYTVIYVKNATEGLN
                     LLAHSLGNKKDYIISTRMEHHANDLPWRENSNILYVEVDELGLLKKEKIEELIKKHKG
                     KVKYLTITGASNVTGYLNPINEIAKMAHDNGIKIIVDAAQLVAHKPINIGGTGNNDHI
                     DFLVFSAHKMYAPFGTGVIIALKELIKDKDPLLKGGGAVDLVSDNKVFLDSEPERFEA
                     GTPNIIGVCSLLSSIKAIKSIGFDKISLLEEDLKKVLLEGLERMPDIITYGDSNYENR
                     IGVVVFNVKDIHHDVISDRLANFRGISIRNGSFCAHPYVRRLLNFKDEEFSKYAYSNK
                     PRPGMLRASLGLYNTKEEIEEFLNTLEIVKEKDFRL"
     gene            18969..19334
                     /locus_tag="K8M77_000963"
     CDS             18969..19334
                     /locus_tag="K8M77_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBI7085474.1"
                     /translation="MKNPSEYHKEGYTCAEAIIKSYNEEFNKDIPVSLGSGMGTGMAV
                     GSLCGAVNGAVLAIGFIKGRESNEEKNEARAYSKELLNRIREEFNSEICLDLKKSKVS
                     CAEIIDFAYDTLKDVLKEK"
     gene            complement(19393..20595)
                     /locus_tag="K8M77_000964"
     CDS             complement(19393..20595)
                     /locus_tag="K8M77_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI7085475.1"
                     /translation="MNCIIAQSGGPTSVINASVYGLVEANKKLNLYENVYGGLNGIEG
                     ILNKKIINLSDLDELQLERFKNSPASGLGSCRYKLKPLSESEDEYLKLLDILNSLNIK
                     AFFYVGGNDSMDTTAKLNAFAKAKNIDIKFFGIPKTIDNDLMHTDHTPGFGSAAKFIS
                     TAVLETYLDSKVYTNNGIFIIETMGRDTGWLAASGALATINGNKCADLVYLPERAFSV
                     QSFLTDVARIFKEKKHVYIVASEGLKDENGKFLTETAPNDDSFGHAQLGGVGAYLKNL
                     IIQASITHRVKSLELNTLQRCSMHASSKIDVNEAVMVGQAALEASVNSEGGYMVAIRR
                     DSSFPYKSSTFLIEADKIANNIKYFPNEWINEEGNNITEEGLSYLKPLISDFEDRDSL
                     PLYHSFVL"
BASE COUNT         8131 a         2326 c         3392 g         7093 t
ORIGIN      
        1 tataatttta taactactat ttaataaaaa ctttttaatt taggcataga tgaaatatat
       61 atgtaaataa aatgtgatat aataattgaa aagaatatat tctcaacaaa gtgagggtga
      121 taaaacatgg gaagaactaa cataagttac ggagctaaga atattttata taagcttatg
      181 ggacaacttt ataaaggaaa accactagaa attttaggcg ttaatgatat gcctaaaata
      241 atggacaggc taccaagaaa cttttttgaa aatgaagttt tagagggagg gcctaatgaa
      301 atatttattt tagaagatgg ctctatttta ctttttggat atgaggatga aaaaaatata
      361 gatgaaaata tgtttatata tttagaggta gctgttaagg ttataaagag atattataag
      421 catagaggaa cttttccaaa ggttagaatg gctataattt ataattcaag tgtaaaggaa
      481 gctagaaata ccttgagttt tggagatata cttatttgtt cagaaagcat atttatgaat
      541 aaactaaatg cggagaaagc tttaaaagag ttaaaggaga aggttttaga agataattta
      601 actcaagatg ataagataaa attcataatg ttgccactta tgaagagtga acatgatgat
      661 agcaatattt taagagattg tgttcagtta gcaaaatcaa taaaatctga aagagatgag
      721 gcaattatat taaatggaat aattgagtct acagataagt ttattagtga agaagaagca
      781 agagaactta aggagttgtt agaaatgact aagattgaga gattatataa tgaagaaaga
      841 gatttagctg ttaaaaatgc aagaaaagaa gagcaattaa gaatagcaaa aagtcttgca
      901 ggtatattat cacctgagtt aatatcagaa aagctaggta taacaataga agaaatttac
      961 gagcttcaac atgcagaagg ctaaagagaa tagaaaaatt tacttttaat aaaaaagctt
     1021 tggatgagaa taatttttag agatgaaaat tgttttccac caaagcttat tttagtattt
     1081 taaataatat atttaaaata tgaacagcat aaagaagata ttcataaaaa tgaaaaatat
     1141 gctataatta aaattattat agatttttta aaggagagaa gataaaaaat ggaactttta
     1201 gtttatgtta aaggaagaag ggatcctttt acttatagtg gtgatagaat agatgtttta
     1261 gattttgaaa tgaatggaat aaagtataag caaataagat attttagaaa aggtttttca
     1321 aaatcagaac ttatagaaag tgaattaata actagaatga gagaaattaa gtagattaat
     1381 tttgccatga ataaaataag cctttactca taaaataaaa ctgatttaag atgaggaggt
     1441 gagatttatg gcaaagaaag atcaacaatc ttcttggaat tcaaaaagat ttaaagcacc
     1501 taaaaagaat aatgaagaac cagtgccaga aattcaaggg aaaaaataga tatatattga
     1561 aaaaacaatg aataagtggc aaaataaaac atagaaaatt tattatatgg gggtgcaggg
     1621 gtgaaaaaat taaataagtt tttatcatta ggtctaatgg tattattttc attaagtata
     1681 ttagtaggat gtaatacttc taagaaagaa gaagctaagg cgcctgaaga aaaaacatcc
     1741 atagaaatag tagtaccaga tggacttcca gctattagta tagttaaaat gataaaagaa
     1801 aaaccagaaa taatgaaaaa cttagatata aattattcaa tagtaaaggg atcagatgct
     1861 ttagtttcta aagtgttaaa aggagaggga gatatatgta tagttccttc aaatgtagct
     1921 gctattgcat ataacaaaga agctaaatac aaacttgcag gaacagtagg ttttggatca
     1981 ttatatgtta taagcagtga taattctgtt aatagcttag aagaccttaa aggaaaagat
     2041 gtttacaatg ttggtcaagg attgactcca gatttaatat ttaagatatt acttcaaaat
     2101 gatggaataa atcctgaaaa agatttaaca ctaagttatg taaatgcagc ttcagaatta
     2161 gctcctttat ttatagaagg aaaagctaaa tatgcagtcg ttccagaacc tatgttaact
     2221 caaataatga caaagaaacc agaaacaaaa atagtagcat cattaaatga acagtggaaa
     2281 aaaatgagtg attcaaaaat gggatatcct cagtctagta ttatagttaa agaggactta
     2341 gcaaaaaata attcagaggc tgttcaaaag gtcttaaagg aaatagataa tagtactaag
     2401 tgggcaaatg aaaataaaga agaagcaggt gcctttgcag aagaagtagg cataacaggc
     2461 aaaaaagaaa taatagctaa atctctagaa agagcaaact taaattatgt aagtgcttta
     2521 gatagtgaaa gtgaatatat taatttttat gacaagattt acagcttaga gcctaaagct
     2581 ataggaggta aaaaggtaaa tgaagaaatt ttcttacaaa aataagaaat taatattttt
     2641 atcttcagtt acacttttta ttttatggca attagctagt atgattatag ggagtgaggt
     2701 aatattacct tctccctatt taacgtttaa tggtttaatt gaagtaataa aatcaagtga
     2761 ttttttcaaa ataataataa atactttagg aagaactttc ataagtttta ccttatcctt
     2821 agtaattgct ttatttttag gcgtgtttag tgcttttaat aaatatgttt ataattttat
     2881 gatacctata ttaaatgtaa tgagatcttt tccaacaata ggctttataa tacttgcttt
     2941 aatttggtta agtcaaggca tagctcctat attagttggc tttgtaatga gttttcctat
     3001 attttatgat gctataatag gagctatatt aaatatagac aaaaatatac tagagatggc
     3061 taaggtttat gagcttaata taggtaattt aataaagaat atatatttgc caagcataag
     3121 ttttgccata tgtagaataa tgatttcaac cttttcttta actatgaagc tagtagttgg
     3181 aggcgaagtt attggacaac ctaaatatgg tataggaacg gcactttttg tggaaaagaa
     3241 ttatttaaat actaatatgg tatttgcatg gataatagta gtaattatta taggaataat
     3301 tttttcttta atacaaaaaa ctattgatgg tagagttttt aggtggatga aataaaatga
     3361 gtattaaatt agtaaatata gaaaagaatt ttggaagtaa aaaaatatat gataaatttt
     3421 ctttaaactt tgaagaagga aaaataaact gtattttagg gaaatctggc tgtgggaaaa
     3481 gcactttatt aaatataata gctaatcttg aagagattaa ctcaggtgaa attataggaa
     3541 ttcctaaaaa aatagcttat gtgtttcaag aggataggct tattgaatgg aatagtatat
     3601 acactaacat ggaacttccc ttattaaaat tttacacaaa ggatgaaaga gaagagaaaa
     3661 ttaaaaatat cttaagagaa gttgaactag gagattgtat gaactcatac cctaaagagt
     3721 taagtggagg catgaggcaa agagcaaata tagctagggc acttctttat aatggggaac
     3781 ttcttttaat ggatgaaccc tttaaatcct tagataaaag tagtaaagaa aatattatta
     3841 agatatttaa aaaaaatcat ttagaaaaaa ataatacagt tttaatggtt acacatgata
     3901 taaatgaggc tttaagctta ggagataata tatttatttt aggtggtaac ccagttagtt
     3961 taatagataa atttaaagat gtacaaaagt caaaagaaaa aaataatata gaagaaaaaa
     4021 ttttacttat tttaaaagaa taagagtata aaggaggggt aaaatgagca aaaaattaaa
     4081 tttactacag gataaagaaa gtacattatt aagaaaatat atgatacctt cagttctagg
     4141 aatgttaggc ttatcagtat gtatattatt tgataccatg tttataggac ataaaatcgg
     4201 agatttaggt ttagctgcac taaatatagc cttgccaata tataatttat atagtgcaat
     4261 aggtcttact attggagtgg gaggagcaac agccctatcc gtagctatgg ggcaaaaaaa
     4321 attccatagg gtaaatagaa tatttacttc ggcagtattt gcaactatca tattttgtat
     4381 aataataagt ttattagaag ttttcttttt agataaaata gtttatatgc ttggagcttc
     4441 agaagcaacc tttcctttag ctaaagaata cttaaagatt atcttagcct ttaatcctgc
     4501 ttttattttt gcatctgcat ttattgtttt tgttagaaat gatagagagc ctaaattagc
     4561 tatgtacgca gtaataggat caaatacaac taatatagta ctagactatg tatttatata
     4621 tatatttaat ttaggaatgt ttggagcagc cttagcaaca tctataggtc aattggtagc
     4681 cttaggagtt ttatcaatac attttataaa gaaaaataac accatgcata tagagcttgg
     4741 aggaataaat ttagacaata taggaaaggt acttaaaaat ggagttccta gtttcttaaa
     4801 tgaaatatca gcaggttttg ttatatttat ttttaatatt gtaatataca aatttgaagg
     4861 cgacttagga gtttcagcat atggaataat tacaaacata gttttaatat tcatggcagt
     4921 ctttaatggg gtatcccaag gggttcaacc tttaataagt gtaaactttg gagctaaaaa
     4981 ggaggagcgt gttaaagaat ttttaaggtt aacaagaata atagtactaa ttttaggtgt
     5041 agtcttctta ggtatgggat tattattacc aaatcaaatg ataggactat ttacaagtga
     5101 tagaggaaga ttattaagta taactagaca gggaatatat ctatacttta tagcattctt
     5161 atttaatgga agcaacatac ttaacatagc ttatttacaa gcagttgaaa aatcaaagga
     5221 atcctctctt ttatcaacac ttagaggatt agtatttgtt attatattta taatagtatt
     5281 accaagaatt attggggttt atggagtatg gctaacaatt ccagtaactg aattaagtac
     5341 acttttattg ttttctatat ttgaaataaa aaagaaaaag atataataaa ataaaaaccg
     5401 ttgattatca acggttttta ttgtaaatgt aagaagtaaa tatagtatag taagagttat
     5461 aatgattttt aaaacaggag gttaagttaa tataattaac ctaataacat actatgaaga
     5521 tttattaaaa tttgctaata aattaaataa aaaattgtag aagttaaata agttgtatat
     5581 aataatatta aagcactttt taaaggagag atttttttaa tggacattgg taattataga
     5641 gagatggatc catatatgtt acttagtatt ataaatttaa aattaagaga ttactatagt
     5701 gatatagaaa gtttgtgtga tgatttagaa attgaaaaag agattattgg acaaagactt
     5761 aaagattgtg gatacatata taatagagaa aataatcagt ttgttgctga ataattataa
     5821 tttatgtttt tttaaaatag ggaggattaa ttaaatgaac ttaagaaaag aaccatattt
     5881 attggtattt ggagcttctg tagtagatgt gtttggattt agtaatgcga gttatagagc
     5941 atataactca acacctggtc atgtaaagat atcttttgga ggagtttgta gaaacatcgc
     6001 tgaaaatatg gcaagagtag gggtaaatac taattttatg tctatattag gtaatgatga
     6061 gcatggaaag agtatagttg agcactcaaa gaaaatagga tatcatatgg atgattctat
     6121 ggtaatagaa ggtggaagca ctccaactta cttagctatt ttagatgaaa atggtgaaat
     6181 ggtttctgct attgcagaca tgaaaagtat aggagctatg aatactgatt tcatagattc
     6241 taaaagagaa atctttgaga atgctgaata tacagtttta gattctgata atccagaaat
     6301 aatggaatat ttattaaaga actttaagga taaaactaat tttatcttag atccagtatc
     6361 agcagaaaaa gcaaactggg taaaacatct tataaaggat ttccatacaa taaaacctaa
     6421 taggcatgaa gcagaaatat tagcaggttt ccctataaca gatatagatg acttaataaa
     6481 agcaagtaat tatttcttag gtttaggaat taaaaaggta tttataagtt tagatgctga
     6541 tggtatattc tataatgacg gtgtatcttg tggtaaaata aaggcaacag aagttgatgt
     6601 taaaaatgtt actggagcag gagactcttt tgtagctgga ttaggatacg gatatatgaa
     6661 taaaatgcct atagaggata ttgttaaatt tgctatgact atgtctaata taacaatatc
     6721 acatgaggaa acaattcatc cagacatggc tttagatact gttttagcta aattagagaa
     6781 aacagcttgg gtagaagaaa aatacgattt aaataaatag aataaaatat tttgtatttt
     6841 cttgtaggct taaactttaa tcctacataa atatataaat gtggtgaggt gagattatga
     6901 gtaaaaaaat aaaaaaagga aaacttgctc tattagccct agcaggaagt gcttttgtag
     6961 catcactgtt atatgataaa aaccaaaaaa ataaaaaaga tatggaaaac ttagaattag
     7021 agttacaaaa tatagaagct aaagaataga aaataaaaga cactagcaat tactagtgtc
     7081 ttttattcta aattaaagaa tttgcttttt ctaaagcctc atcaaaattt gtagcaaagt
     7141 gatcttcacc taaagtatat ataaatccca taagtctcat aacttttttt ggttgttctt
     7201 gtaactcaga tattattaaa gtcatattac gagacttaca acttagagct atatttttta
     7261 tttgtttata gcctgtaaca tctaaagtct tagtatgtct cattcttaaa acaagaacct
     7321 ttgcatcatc atttatttcc ttcattgtat tcataaatgt atctacagca ccaaagaaaa
     7381 ggtgacctct tatatcataa actaagacat tttctccaac tttaagattt tccatatcag
     7441 tatctatatt agatttataa gaacagtcac tttcatttaa ttcatttact tctatagaag
     7501 tagcaactct tcttataaat aagcacatag aaactatcat tcctatcact atagcaatta
     7561 ctaaatcaaa taatactgtc aagaaaaatg ttattagtaa tatagctata tcacttttag
     7621 gagccttaag tattgcttta aaagttctcc attcactcat gttatatgaa acaattatta
     7681 atattgctga taaagtagtt aatggaatga atttagcaag aggcataaat acaagcatta
     7741 taagtaataa agttattgaa tgaactatac cagaaatagg agttcttcct ccatttttaa
     7801 cattggcagc agttctagca attgctccag tagcaggaat tcctccaaat aaagaagaac
     7861 caatattagc taatccttgt gctataagtt ctgcatttga attatgcttg tctccaatca
     7921 taccatctga aacaacagca gataataaag attcaattcc acctaaaaga gctattgtaa
     7981 aagcaggtcc tattaatgct tttaatgtat taagatttag gttaggaata tgagggattg
     8041 gaatatttga gcttatttta ccaaattgac ttcctattgt tgctacaggt aaattaaata
     8101 taaatactac taaagttgtt actattaagg ctattaaaga tcctggaatc tttttattta
     8161 tttttggcca aaagattaaa ataataagtg ctagtaatcc tatagctaag gtataaaggt
     8221 ttgtagtatt catatgagat atgtaagctt cccacttagg tataaactca gagggggttt
     8281 tagtaattga aagtcctaaa aaatccttaa cttgtgttga taaaagagtt atagctatac
     8341 cagaggtaaa tcctaccgtt attggataag gtatatattt tattaagcta ccaaatcgta
     8401 ataaaccaaa taaaacaaga ataataccag ccataaatgt ggcaattatt agtccatcaa
     8461 ctccatggtt ttgtataata ccaaatacta taacaacaaa ggcgccagta ggaccaccaa
     8521 tttgaactct acttcctcct aataatgaaa ttatgaatcc agctatgatt gcagttatta
     8581 atcctttttc aggagatacc cctgaagaaa tacctaatgc aatggatagg ggaagtgcta
     8641 taatagcaac tattatacca gcgattaaat cttttaaaaa ttgttctttt gaaaatccgc
     8701 tctctttatc atctaaaaga gaaattaatt ttggtttgta cattgaaata tatcagctcc
     8761 ttataaaaca gtcgatttct attatattac ttaaatggta tatttacaat ttcgtagaac
     8821 gatttgaaac gttattaatc aaggaagaac tgttttgttc acaaaaagtt tacataaatt
     8881 atattaaagg taacctatgt tacagaagaa ttgggaaaga taaaatataa tttacttata
     8941 aattatatga agaggtgaaa gtatgagttt atttttagga aaaatccatt tttggttatt
     9001 tgataaaata aagtggtttg aaaatctaga ggaagaagtt ttaaaaattg caaaggaaag
     9061 aaatatgcca gtagaggagt ggatttctta tgcaaattta aattttggag aaaaaactcc
     9121 aaataaacct ttagatgaaa taatagatga aagcaatata catggttggt tagaaggaag
     9181 aataaattca gctgagagta gatgtgcata ttatataact aatatgttaa aggaagatag
     9241 tgaagtaaag aaggagctta tagagcttta tgaaaaccat ggaaaaataa atgcagacga
     9301 gtgtaaaggg caaatagatg gggaaaatat tttagaagtt tacaattctt taaatgatta
     9361 tatattagat ggtatgcctt gtgatagaat taatgaggtt ttagaaaatt ctcctgaaaa
     9421 aatagtatgg catatgagta gagacctaca tgaaagattt tgggaaagtg taggaggaga
     9481 tgtaaatgag tttcatgatt taagaaattc atggataaaa gcttttgttg aagaaattaa
     9541 tccacaatta gaattagtta tttatgaaaa tggacataag gctatagtaa gaaaataggt
     9601 tagactatta atttttaaat attataaata aaagtatagt gaaataggta agataatggc
     9661 actttgacaa ttaagtacat attataaatt aaaacccttt aacataggag gataatatgt
     9721 atttagctca aggtgccatt ataagtttgg acttaggtaa agggcatatt atagacaaag
     9781 atacatcaga tgatttaaaa gaaaaaattc aaagaactat tctttatttt tttaagaagg
     9841 aagtagataa aaataattta aagtttattg attttactcc ttataataaa ttttttattt
     9901 caaatggaga aaagttaaaa agcatagtga aaagagtaaa tagaagtgaa tcagatttgc
     9961 atatcacttt aaacattaat ttttcaaagt caaatgagat tttttgtttt gtagaagaat
    10021 ttgattcaaa agggaaaaag tttgcagaga atatatgctc attttttgaa ttaatcaatt
    10081 ataagaataa gggtattaag gagtcagatg tctataatct tttgtatata gataaaccaa
    10141 gtataataat agacttgaat ttaaacatta atgatgaatc tgaagtggat gaaaaagggg
    10201 aatgcatagc taaaacttta gttgaatcta ttttatcctt aggaacaaaa taaatgttga
    10261 gagagaatct caacatttat tttttaatct ataaatttca ggagtgcttt catctattac
    10321 ttcaaagcta tatccagctg atttaaaatg ttttatgatt gatggcaatg ctttagcaga
    10381 attattatta gaatatccac aatgcattaa aagtacaata taatcagaat tagaaattga
    10441 cttttgaact atagaacttg ggcttaggtt tggattaagg gcatcagtac tatctactgt
    10501 ccaatcatat attctaaagc cttgttcatg taaggagtct actaaggatt tttttaaggt
    10561 atacgtgcta ttattagagc caaatggaaa tctaagagaa aatactttct ttccaataac
    10621 tttttctatg gatccttgag tttttaagtt ttcttctagg aatgaagagt catttctgta
    10681 aattttattt ctctcatggg taaaggtatg tactcctatg gaatgaccct catcattcat
    10741 tctcttaact aaatcagggt gtttttctac tagttctcct attaaaaaaa atgtagcttt
    10801 tacatcattt tctttaaggg tgtttaaaat ttcttctgta accttaccac cagggccatc
    10861 atcaaaggtt aaaaatattt tttttgtgct ttcttctaac tcagctgctt taataggttt
    10921 tgaagaggta gctaaggctg aaaaagaaaa tattaatgta attagtaaaa aaacaaattt
    10981 tttcatttta tcacttcctt aaatattatt tttatattta aaacatatta attagtattt
    11041 tcaaaaaaag cattaaatat aagcatatta ggttaagaat tagaaaggaa taattgtttt
    11101 ttatttcttt cactttatgt tataattaat aaaataaaaa atataaagag agtgataata
    11161 tatggagaag tatttaagaa tattattagg aataactgtt acaatactta tattcgcttt
    11221 tgcgatttgg atattacctt atgttttaat aggagttgca atactgatcc ttataggatg
    11281 ggtgtacttt aaatttataa gaaagtatgt taataaaaat aaaaaagaaa aaacagaaac
    11341 ctatacttat acatttacaa aagaagaaaa gaaatcagag gctgatatgc aagatgatgg
    11401 tcctgttatt gatgttgatt ttgaagaagt aaaagatgat aaataaggat tgatttaatg
    11461 aattttagaa aattaaaaat attttttgaa acagctaaat gtttaaatat gactaaagta
    11521 gctaagtcta tgtatataag tcagccatct ataagtcaag ccatagctga attagaaagt
    11581 gatcttgatg ttaaactttt tgatagaata ggtaaaaggc tttatcttac tcatgatggg
    11641 gaagtatact ttgaatatag tagaagaata ttaaatcttt atgaagaggc aaatagtata
    11701 attagaagta gcaaagaggg tcaaaaaggg aaaataatta taggagcaag tacaacgata
    11761 ggtatatata tacttccaaa attaataaaa gaatttaatg agttacataa aaatatagaa
    11821 atctccttaa taatagaaaa tacacagtta atagaagagc ttattattga gaacaaggtt
    11881 gatattgctt tagtagaagg atatgtaaaa tcaaatgaat tagaagtttt tgacataggt
    11941 aaagatgagt tagtatttat atcaaatcct aataatccta tatttttaaa ggataaaata
    12001 actttaaaag atttagaaaa tgaaaatttt attatgagag aatctggaag tggtacaaga
    12061 gaaattatag aaaactatct tataaagcaa ggatgtaact acaatgtata tatggaactt
    12121 ggaaatacag aggctattgt aaaagttgta gaaacaggtc ttggtatagc ctgtgtttct
    12181 tgtaaggcta tagaaaaaag aattaatgat ggattaatta aagaaataaa aatagatgat
    12241 attaaagtaa atagagattt atatcttata tatcataagg ataaatttat ttctagaaat
    12301 ttgaaaatat ttatagataa aattaaaaat tcagatatat aaaaaaaagg atagcaatac
    12361 ttatttttta gtattactat cctttctttt cttttatagt agataaaaga aaattgatat
    12421 aaagtgacat aggcttccta ataatatgaa tatatgaaat atttcatgga atcctaagtt
    12481 attaaattca agaaattttg gtttgagtgc ataaattatt ccaccaattg tatatataac
    12541 tccacctatt gctaaccaaa ttaatgctaa agcaggtatg tttttgaaaa atggaatagc
    12601 acctaaagaa atccatccca ttcctatata cataagagta gaaagaattc taggacaatt
    12661 aaaccaaacc atcttaaata aaatacctaa aatcgtaaga attactatta aagaagttat
    12721 aaaaatttga gctttacctt caagaccaat aagacaataa ggcatataac ttccgcagat
    12781 aagtataaag atcatagaat ggtctaaacg tcttaaaaat gctatagttt tttcctttgc
    12841 attaactaaa tggtatatag cagaggcact ataaagcaac actaatgaaa aaccaaatag
    12901 aataactgat aaaagtggta atccaatccc gtatatataa gtagctttag ctgttagtgc
    12961 cactaaggcg aatattgaca aaatagcacc gaataaatga gtaaaaccat ttactggttc
    13021 tctaaaaaac atatttttca taacatacct ccaatgaaaa gtttttaagt agttttaaaa
    13081 actacttact atcatactac aattatcatt taaatatagg aatatatcaa cctttaaatt
    13141 agtgagaatt ttaatgatat atagataata ttgccaaata gcttgaattt gttttaattt
    13201 ccttataata taatgtatat agggtgaaat gtagttttga aaactatttt ataaattgaa
    13261 tggaatggaa gtgattttat gaatttaaat gatttattag aaagtttaaa tttagataat
    13321 aatattaagt tagaggatat accagaatta gatttatata tggatcaagt aatacaatta
    13381 tttgaaaata aattaggggg aaataagagg tttaaagaag ataagatttt aactaaaact
    13441 atgattaaca attatgccaa gggaaacttg ttaatggata taaaaaataa gaagtatagt
    13501 aaggaacata ttatacttat gtgctttata tataatatga aggggatatt agctcttagt
    13561 gatataaaac tagctttagg agatattgtt gaagggtata ataaggagga atttaattct
    13621 gaggatataa gaaagttcta tgaaatatat ttaaagggac aaaaaaaaaa tgtaaaagat
    13681 ataaaaagtg aaattaatat agatctaaat aatttagggg aagaatttaa ttctttagaa
    13741 agtagtgaaa agaaattact tttatctgca attctggtgg ataaggcaaa tatatataaa
    13801 aaactagctg aaaaaatact agacagtaag tagaaattag aataaaaagt aacactttat
    13861 aattggtagt cataatatat attaatcata tgataaaacc taggagggta aattatggat
    13921 tataaagatt gccaatgcaa ttctagagga atagaattaa atgaattaga agaattattt
    13981 gaagatgtaa gatgtaataa ttattataat agttgtgata attatcataa gtatgatcat
    14041 tgtgatcatc ataatcattg cgatcatcat catgaatatt gtgatcatca tgataattgc
    14101 gatcatcatc atgataattg caatcatcat gatcataatc atggatgctg tgacgataga
    14161 agagattgtt gtaagagacc tcaaccaaaa ccaattccta agaaaggaac tattgaagta
    14221 caatctttat taggatgttc tgatggtgaa ccaataagtg gaatgccaat agaattatat
    14281 aaagtagatt gtaatactgg ttttgaactt gtagattgta aatatacaga tcgttgtggt
    14341 aaggtttgtt ttaaatgttt agaagatgga ttatatgctg ttaaacaacc tgtagatcgt
    14401 tgctactttg aagcaccaga atactatcca tgctatgaag tatgtatttc tccaagaaat
    14461 aaatatgtta aagtagtaat aataaacaaa ttaagagata tggaagactg taatagacca
    14521 cctatacatt gtagatgcaa tagtgaagca agctgtcaat aattagaata aaattattta
    14581 gaagctcaca gaatattttg tgggctttaa tttttagaaa taaaattata taatagctat
    14641 agtttaattt tatttctaaa aaattatata gattaaacaa aagaaaggaa tttgatatga
    14701 taattagaat taagtatttt gataatgcaa ctaaattaaa aaagataaca aagggtaatt
    14761 ggatagatat atatgctaat aaggatatat ttgttaaatg tggagagaga gcaatggtac
    14821 ctttaggatt tgctttagaa cttccagaag gatgggaagg acatttagcg cctagaagtt
    14881 caacttttaa aacttgggga attatacaaa ctaatagtgt tggtgttgta gatgatacat
    14941 atataggaga taatgatcag tggcatatgc ctgtttattg cttacaggga aaagacattg
    15001 aaagtgagaa tggggaagaa gttaaaggaa cttggataag aaaaggggat aaaataggtc
    15061 aatttagaat tatggaagtt atgccagaaa tagagtttga agaggtagaa tcctttggaa
    15121 acaaagatcg tggtggattt ggaactacag gaacaaaata gataaataaa gaagtagtgt
    15181 attaaaataa ctttaagaaa tttattttaa tacactattt tatttaagtt caatttaaaa
    15241 gagataatac atggaaattt tttttgaaga actttccatg cagtctcttt tttagattgt
    15301 tatatggata aattatttta ttattttatt cttcaagaga ttcagaaagc tcttcccaag
    15361 tagcgtaaag agaatctatt tcttcttgaa ctgaattaat ttctttattt actcttacac
    15421 tttcttctgg atttgaataa acttcctcag cacaaagcat tccttgaagt ttttcaagtt
    15481 cttcttcttt ttgaggaatt aatttttcaa catctctaag ttgagctctt aaagctctag
    15541 ctgatttttc agcagatttt ttcttttttc tttcttcttt tatttgagtt tttgtttttc
    15601 cttggtttat ttcctcatca atatcttcat atctattagg attagctttc ttatccatat
    15661 aatagttgta atttcctaag tatgttttaa gaccattttc ttgaagttca acaattttgt
    15721 taattacttt atttaaaaag tatctatcat gggagattac tattaaagtt ccatcataac
    15781 ttaaaatagc atcttctaag gcttctctag acataatatc taagtgattt gtaggctcat
    15841 ctaaaagaag aagattggcc tttgaaagca taagttttaa aagatttatc ttacactttt
    15901 caccaccact taatagggaa atttctttaa atacatcatc accagtaaat aagaaagaag
    15961 caagataatt tctaatctca gtagtagtaa gattagggaa gtcatcccaa acttcatcta
    16021 ttatggtttt atggagattt aaattagatt gttcttggtc ataatatcct gtaaatacgt
    16081 ttttaccaag ggatacagaa ccagaatcct gttttaacat tcccattatt attttaaata
    16141 gagtagtctt acctctacca ttttccccta ttagggcaac cttttctcca cgttttaaat
    16201 ctaaatttac attttcaaat aaaagactat catagctctt ttttaggttt tcaatatgta
    16261 aaacatcatt tccactttta actaaggttt caaagcttat tttagaagcc tcttttataa
    16321 cctctggtgc ctctagacga tccattttat ctaaagcttt ttgtctactt tcagcagctc
    16381 taacactttt ttctctatta aatgaacgga acttttctat aatttcctct tgtcttttta
    16441 tttctgcctg ttgaagatta taagctttta atctagcttc aatatccttt tttctaagtt
    16501 caaggaattt agtatagctt gcattataac aatgtatctt tccaccaata agttcaaagg
    16561 ttgtatttgt tacagagtct aagaaaaatc tatcatggga aataacaaaa accgttccct
    16621 tataaccatt aagatattct tcaagccatt ctatagcttc taaatctaag tggtttgtag
    16681 gctcatctaa aagaagtaag tctggttttc ttaaaagtag tttacagagt gctaatctag
    16741 ttttttgacc tccactaagg tttataattt gattgttgaa atcttcctct aaaaaaccta
    16801 agcctttaag aactctagaa atttctcctc taaaagtata acctcctcta ttttcataaa
    16861 gttcttgaga taaggtataa tcctttataa gtttattatg atattcttcc tttgaagcat
    16921 cataaggttc attaagtttt tcttctaagg ttttaatttt atcttcaagt tccgttaaag
    16981 atgagaatac agaaagcatc tcatcataaa tagtattttc actatctaaa gataaattct
    17041 gagagagata tcctagggtt ttgtttttat ctaaaaataa ttcaccacta tcataatcta
    17101 gttgcttggt aagaatttta aataaggttg atttaccttc tccatttggt cctattaaac
    17161 caaccttgtc accttcattt atattaaatg ttacatctct taaaatctca tcaataccaa
    17221 aacttttagt taagtgtcta cagcttaaaa taatcatttt aaaactccta tcatcgaatt
    17281 tatagtatta atcacataca attatactaa taatgaagaa tttcatcaaa ttaaatgttt
    17341 gcttagaaag gatttcatga aatttgtata ttttaagaac ctaatttaaa cctttagaat
    17401 aatatatacc attggagtga tgtgttatga ataataattt tagagaattg tttataggcg
    17461 ttgatgaaga aataactatt tacaatggaa aaaagatagt accaattaat cttgataatg
    17521 cagctactac ccctgcttta aaggaaggat taaaatgcgt tagtgacatg ttgcatgtat
    17581 atagttccat tggaagagga aaaggttata aagcaaattt ttgtaatgaa ttttatgaaa
    17641 aatcaagaaa aaagatttta gagtttttta atgttaagaa tacagaggat tatactgtaa
    17701 tatatgtaaa aaatgctact gaagggttga atcttttagc acattcctta ggaaacaaaa
    17761 aggattatat aataagcact agaatggaac atcatgcaaa tgacttgcct tggagagaaa
    17821 atagtaatat tttatatgtt gaagtggatg aactaggact tttaaaaaaa gaaaagatag
    17881 aagaattaat taaaaaacac aaaggaaaag taaaatactt aacaataaca ggtgcctcta
    17941 atgtaacagg atatttaaat ccaataaatg aaatagcaaa aatggctcat gataatggaa
    18001 ttaaaataat agtagatgca gctcagttag ttgctcataa gccaataaat atagggggaa
    18061 ctggaaataa tgatcacata gattttctag ttttctcagc tcataaaatg tatgctccct
    18121 ttggaacagg ggtaattatt gcattaaaag agcttataaa agataaagat ccattgttaa
    18181 aaggcggagg agcagtggac ttagtctcag ataataaggt ctttttagat agtgagccag
    18241 agcgttttga agcaggaacc cctaatataa taggagtatg ttcactttta tcgtcaatta
    18301 aagcaataaa atcaataggt tttgataaaa taagcttatt agaagaagac ttaaaaaaag
    18361 ttttattaga aggtttagag cgtatgccag atataataac atatggagat tctaattatg
    18421 aaaatagaat aggagtagtt gtatttaatg ttaaagatat tcaccatgat gtaatatcag
    18481 acaggttggc taattttaga ggaatatcta ttagaaatgg tagtttttgt gctcatcctt
    18541 atgttagaag acttttaaat tttaaggatg aagagttttc aaaatatgct tatagcaata
    18601 agcctagacc aggaatgtta agagcaagtt taggactata caatactaaa gaagaaatag
    18661 aggaattttt aaatacttta gaaatagtaa aagaaaaaga ttttagattg tgaatctcat
    18721 tctaaataaa ttcttaagtt ttcatatata agaatttatt tagaaatttt ttgatttaaa
    18781 atcacagaaa taaaaagaaa tttttgatat tataaattat ataattaatt ttatattaaa
    18841 ttaaaaagta aaagttaaat attctattta gaagttcttt actagtttaa tttaataggt
    18901 tacaaatatt ataaataatt atcttttgtg aaatatgcag aagatatttt agaaagaagg
    18961 agaaaaatat gaaaaatcca tcagaatatc ataaggaagg atatacttgt gcagaggcta
    19021 ttataaaatc ttataatgaa gaatttaata aggatatacc agtatctcta ggaagtggta
    19081 tgggaacagg catggctgtt ggaagtttat gcggagccgt aaatggagca gttttagcta
    19141 ttggtttcat aaaaggaaga gagagtaatg aggagaagaa tgaagctaga gcttattcaa
    19201 aagaactttt aaatagaata agagaagagt ttaattcaga aatttgttta gacttaaaga
    19261 aaagtaaggt aagttgtgca gaaataatag attttgcata tgatacttta aaggatgttc
    19321 ttaaagaaaa ataaaagaca tgcattttct gcatgtcctt tattaaatgt aggtaattga
    19381 gaaaaataat atttataaaa caaaactatg ataaagagga agagaatctc tatcctcaaa
    19441 atcgctaatt aatggcttaa ggtatgataa accttcttcg gttatattat taccttcttc
    19501 atttatccat tcatttggaa aatattttat attattagct attttatcag cttcaattaa
    19561 aaaagtagag ctcttatatg ggaacgaaga atctcttctt atagcaacca tatagccacc
    19621 ttctgaatta acagaagctt ctaaggcagc ttgtcctacc ataacagctt catttacatc
    19681 tattttagat gaagcatgca tagaacatct ttgtagagta ttaagttcta gagatttaac
    19741 tctatgtgtt atactagctt ggattattag gttctttaag taagcaccta caccacctaa
    19801 ttgagcatgt ccaaatgaat catcatttgg tgcagtttca gtaagaaact ttccattttc
    19861 atcttttaat ccttcagaag ctactatata tacatgtttt ttttctttaa atattctagc
    19921 gacatcagtt aaaaagcttt gtactgaaaa tgctctttca ggtaaatata ctaaatcagc
    19981 acatttattc ccgtttatag ttgctaaagc accagaagct gctaaccaac ctgtatctct
    20041 tcccatagtt tcaattatga aaattccatt attagtataa acttttgagt ctaaataagt
    20101 ttctaaaact gctgtagata tgaattttgc agcgctacca aatcctggag tatgatctgt
    20161 gtgcattaaa tcattatcta ttgttttagg gattccaaag aattttatat ctatattttt
    20221 agcttttgca aaagcattta atttagccgt agtatccatt gagtcatttc cacctacata
    20281 aaagaaagct tttatattaa gtgaatttaa aatatctaaa agttttaagt actcatcttc
    20341 actttcgcta agaggtttta atttatatct acaagaacct aaaccagagg ctggtgaatt
    20401 tttaaatctt tctaattgaa gttcatcaag gtctgaaagg tttattattt tcttatttaa
    20461 aataccttct attccgttta atccaccata aacattctca tataaattaa gttttttatt
    20521 tgcttcaact agaccataaa cactagcatt tattactgaa gtaggtccac cagactgagc
    20581 tataatacaa ttcatttgaa aaatctctcc ttttattatg cacatttata taaaaaatgt
    20641 taaatgtgct atactattaa acttaataac gtactctatt tcataatata cactacctta
    20701 atatttattg tcaaataaat ttttgattta aattaaattt taaagtaatt agtgtttatt
    20761 ttagtgattt taaagaaaat aagattttat taaacattat ttggttgatt tttgttcaca
    20821 tagtgatata attagtaatc aagaaaggat atattatagt tgcaaatatg caattttgat
    20881 atatcctttt gcatttcaat tttttaaatc ataagtaaga ctaaattagt ttaataatca
    20941 aa
//
LOCUS       DAEVGI010000029        20872 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEVGI010000029 DAEVGI010000000
VERSION     DAEVGI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 20872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20872
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.029"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(25..1062)
                     /locus_tag="K8M77_000965"
     CDS             complement(25..1062)
                     /locus_tag="K8M77_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI7085476.1"
                     /translation="MFRVLKDKKRFIKKLILIFIIILFIFLLYQFEAFRDIIFIVLVS
                     GIFAYILKPLYRFLCERTKINKNFLAMSIVLSVIFLILFFLTVLIPSMFKEGESFDGL
                     INGVELFINDLIMKMKFMELGIFDVIEAQVTEKLNILLVSFATSIINNLISFSENILA
                     FAVIPVLAYYFLAYGDLLSNKFLYCCPMEKRALLKNLGRDVDKVLGKYILGQLLLSLL
                     VGIMTFIGMLILGIKFPLLLAFLNALLNIIPYFGAVLGAIPAIVVALVEGPNKILWVI
                     LTFLIIQQIEGNLIAPKITAESIDMHPILIIILLLIGEQIGGLLGMVFIVPIAVVIKV
                     LFDDWDYYMFL"
     gene            complement(1088..1561)
                     /locus_tag="K8M77_000966"
     CDS             complement(1088..1561)
                     /locus_tag="K8M77_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HBI7085477.1"
                     /translation="MYRIRDLIGLKVFDSNGKKVGVVCDLAIDYFNGKVMGFIITKKL
                     FSKRDYVDLTNVISLSDTIMTEGVHVHRGLKFSEIKNFDLINKDGHMIGILEDILIDY
                     DFYIRGLIVVSGLVDKFKNGKRVIQLKETILGEESILFFGNKNINMRMIPRKYFG"
     gene            complement(1843..2922)
                     /gene="mnmA"
                     /locus_tag="K8M77_000967"
     CDS             complement(1843..2922)
                     /gene="mnmA"
                     /locus_tag="K8M77_000967"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBI7085478.1"
                     /translation="MTKKKVVIGMSGGVDSSVAAYLLKEQGYDVIGVTMQIWQEDKEY
                     EEREGGCCSLSAVDDARRVAQKLDIPFYVLNFRDSFKRNVIDYFVDEYIQGRTPNPCI
                     ACNKYLKFDELLQKAKGIGADYVATGHYAKIEERDGRFQLIRSKDDRKDQTYALYNLT
                     QEQLEHTLMPCGEFTKDKIREIAKEIGLDVHNKKDSEEICFIPDNNHGRYICEAAPNK
                     VRTGNFVDKYGNVLGKHKGIVYYTIGQRKGLGLALGRPVFVTDINPVTNTVVVGPEED
                     IFKTDLVCKDINFISIDKLEDPMEVEAKIRYSAKPAKATISPMENGRVKVSFEDKQRA
                     ITKGQSVVFYKDDLVVGGGIIESLL"
     gene            complement(2941..3375)
                     /gene="nifU"
                     /locus_tag="K8M77_000968"
     CDS             complement(2941..3375)
                     /gene="nifU"
                     /locus_tag="K8M77_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBI7085479.1"
                     /translation="MIYSEKVMDHFKNPRNVGEIKDANGIGEVGNAKCGDIMRIYLKI
                     ENNIVEDVKFETYGCGSAIASSSMATELIKGKTVEEAWELSNKAVAEALDGLPPVKMH
                     CSVLAEEAIHKAINDYRQRQGLEPWDMKEHSHDIHEHVHGQE"
     gene            complement(3378..4574)
                     /gene="nifS"
                     /locus_tag="K8M77_000969"
     CDS             complement(3378..4574)
                     /gene="nifS"
                     /locus_tag="K8M77_000969"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBI7085480.1"
                     /translation="MNNRVVYMDYSATTYVKPEVLEEMLPYFTNKFGNPSAFYGVSRE
                     SRMAVDTARERVAKVLNADTNEIYFTGGGSEADNWAIKGIAFAHKNKGNHIITTKIEH
                     HAVLHTCQWLEKQGFEVTYLDVNEEGFVDLEELKNAITDKTILVSVMFANNEIGTIEP
                     VKEIGKICRERKVIFHTDAVQAVGNVKIDVKDMNIDLLSLAGHKVYGPKGIGALYIRK
                     GIRIDNLIHGGGQERARRAGTENIPAIVGLGKAMEIAGENLDEHIAKISKLRDKLIKG
                     LLEVPFTRLNGPKDGSKRLPGNVNVCFEFIEGEGILLSLDFEGICGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLSLGEGTTEEDVDYVLEKVPPIIARLRSMSPLWKNHLR
                     EVEGEN"
     gene            complement(4567..5028)
                     /locus_tag="K8M77_000970"
     CDS             complement(4567..5028)
                     /locus_tag="K8M77_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBI7085481.1"
                     /translation="MKLSTKGRYGVKAMVDLAINYGGSPVSIKSISQRQNISEYYLEQ
                     LFSSLRKAKLIKSIRGAQGGYILNRQPEDITVSDIIEVLEGPIEISDCLDGVTCNNVD
                     CCATRLLWKKIKTSIDEVTNSVTLKDIVEDYKAMKEKNEALKIVSRSEENE"
     gene            complement(5158..6405)
                     /locus_tag="K8M77_000971"
     CDS             complement(5158..6405)
                     /locus_tag="K8M77_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBI7085482.1"
                     /translation="MRPLADIMRPKKLEDIVGQKHIVGEGTPLSNLIKNKNMINCIFY
                     GPPGVGKTTLANIISNYTDKKFYKINATTSSIKEIQSITSDIDNLLNFKGIILYIDEL
                     QHFNKKQQQALLEFIEDGRVILIASTTENPYFAIHKAILSRSTIFQFKPVSTEDIIIA
                     LERAVSKLEKEGYVISLEEGVLKYIGEISQGDVRKAYTILELAIKSQEKDNILLNKEY
                     VEKLGQSNLKSDSSGDDYYNLLSALQKSIRGSDPDASIHYLARLIKGGNMDSVIRRIG
                     VIAAEDIGLAHPNALQVVNSGIDLALKIGMPEARIILSELVIYLATLPKSNSAYMAID
                     DALRDLERNNSGDVPKHLKDAHYSGASNLGVGGYLYPHNYPNSYINQEYMPENMRGKE
                     YYKPKDNKYESSIKAYWNNVKNK"
     gene            complement(6607..7506)
                     /locus_tag="K8M77_000972"
     CDS             complement(6607..7506)
                     /locus_tag="K8M77_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI7085483.1"
                     /translation="MEVLLDVKERMSILKNFYDEVRVVDPINNIVFSDDFNLKINNEF
                     NEKDCSLSKCYDIWGKNSACSTCISKKAYLKNDTFIKLECNKNHVFLVIASPIEFNEN
                     KYVVEILKDITNNGSIVDGSGDNNYLNLTIKKIGENLVKDHLTEVYNKRYIDQKFSKE
                     SNRNLKESIPTTVIMADIDSFKKVNDTYGHLVGDKILRDFAKVLNDNIRENNDWIGRY
                     GGEEFIIVLNNTNLENGVKVAEKLRKIIEKMSFNYGDLSIKITSSFGVCEISEKEDTF
                     DTIKNADEKLYMAKMTGRNKTVF"
     gene            complement(7692..8120)
                     /locus_tag="K8M77_000973"
     CDS             complement(7692..8120)
                     /locus_tag="K8M77_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBI7085484.1"
                     /translation="MSKVRAICISEKKGTAKVKVPKAEFIEDFGIKGDAHAGKWHRQV
                     SLLAFEKIEDFRADGGNVDFGAFGENLVVDGIELNKLPIGQKIKIGEVLLEVTQIGKK
                     CHDKCAIYYQVGRCIMPAYGIFTKVLNGGEVILGEEVELL"
     gene            complement(8123..9094)
                     /gene="moaA"
                     /locus_tag="K8M77_000974"
     CDS             complement(8123..9094)
                     /gene="moaA"
                     /locus_tag="K8M77_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBI7085485.1"
                     /translation="MKDKYGREIDYLRISLTDKCNLRCAYCMEKDHNDFIHNDKLMTL
                     DEILRVVKECASIGIKKVRLTGGEPLVREGIVDLIKNINKIPEIEEICLTTNGILLGD
                     KVKELSENGLKRVNISLDTLKEDRFKEITRIGTLDKVLYSIEKCLENNVKVKINTVIL
                     EDFNKDEILDLINLAYKNPIDLRFIELMPIGEGKKFKGVTNSEILEIIKKEKKVLSDG
                     KTLRLNGPAKYISIEGFKGKIGFISAMSDCFCEDCNRIRVTPEGFMKQCLHWKYGINL
                     RDKMRNGISDEELREIIKKSIYEKPEKHNFKMKEKDEDKRFMYEIGG"
     gene            complement(9240..10253)
                     /locus_tag="K8M77_000975"
     CDS             complement(9240..10253)
                     /locus_tag="K8M77_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBI7085486.1"
                     /translation="MKQIRVEDAVGTILSHDVTQIIPGKFKGRAFKKGHVIREEDIEK
                     LLSIGKEHVYVWEKEEGMLHENEAAERLKDLVCGEGLSFGEIKEGKIEFLADKEGLLK
                     IDKEKLLELNMLGEIIVSTIHGNFPVKKGDKVGATRVIPLVIDENKIIEAENLIKDKI
                     IEVKEIKNKKTFAITTGNEVYSGRIKDAFGPVLKEKLKEFNIDLERQVILPDDKEKII
                     DEIKRALDEGAELILCTGGMSVDPDDVTPTAIKECGGELITYGSPVLPGAMLLLAYYN
                     DVPIIGIPSCAMYSKRTALDLVLPRVLADERLTLRDIAEYGHGGLCLNCPVCTFPHCS
                     FGK"
     gene            complement(10372..10968)
                     /locus_tag="K8M77_000976"
     CDS             complement(10372..10968)
                     /locus_tag="K8M77_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBI7085487.1"
                     /translation="MIKKSAAILAGGKSSRMNYINKAFLKYEENYFIERIIKALEDYE
                     EIIIISNNPEEYTEFGLKVFKDIYPSQGPLSGIHSALNYIKNDYCLVVACDMPFINKE
                     VVNYLGNIKEDYEILIPKFQERLQPLCAIYKKSCKDIMEKELINNSNKLIKTCFKFSM
                     KVVEEFPFIEKIHKKEIKNFYNINTVHEYEDLIRKKEI"
     gene            complement(11012..12232)
                     /locus_tag="K8M77_000977"
     CDS             complement(11012..12232)
                     /locus_tag="K8M77_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBI7085488.1"
                     /translation="MKKFIALEEALEILNKNTKALKSEVVSIKDSLKRVLYGDVKSKI
                     NNPPFNKSVFDGYTFKSEDSKGTSKENPIELKIVDEIFAGMWPEIEIKHGEAVRIMTG
                     APIPVGADCVLKQEETERHGDLVKIFKEMKSYENISFMGEDIKIGETLIKKGKRLDYA
                     DLGIMASSGISEVLVYKKPRVSIISTGDEVCDINSTLKPGKIYDSNLYSLSARIEELG
                     YNVLSMEHVGDNILKIGEDIEKAFEKSDIVFTTGGASVGEKDLMQKVSESIGFEKLFW
                     KIKIKPGSAVVCSKKEEKILISLSGNPNAALTTFELLGKSVLKKLEGEEENINIKREK
                     GVLMDSFNKKSPQRRFLRGNVIYDEKGAKVYITQIKSGNGILSSLLNANCLIEIEKGN
                     EGLNRGEVVSIIKL"
     regulatory      complement(12381..12540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(12536..13018)
                     /gene="mobB"
                     /locus_tag="K8M77_000978"
     CDS             complement(12536..13018)
                     /gene="mobB"
                     /locus_tag="K8M77_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBI7085489.1"
                     /translation="MAKIVNVVASCSNTGKTVLIEGLIKELKNRGYTVATIKHDVHGF
                     DIDKEGKDTWRHRKAGAEAVIISSKERMALIREVQEEVPLEELIKQVEDFDFIIIEGY
                     KNSKYRKLEVYRQGISKKIITPKEKLIGIASDVSLSLEGIRVVDLNNYEEIANLVIEN
                     "
     gene            complement(13109..14332)
                     /locus_tag="K8M77_000979"
     CDS             complement(13109..14332)
                     /locus_tag="K8M77_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7085490.1"
                     /translation="MRYIVVGASAAGISGAKTLRELDKDAEIILVSKDENVYSRCILH
                     HYISVHRDIEALDFTDRDFFEKYNIEWKKGLEVKSIDDREHVIGLSNGESLKYDKILL
                     ATGASAFIPPVENLREAKNVVGLRNLEDAIKIKEEAEKVKNVVVLGAGLVGIDAIAGL
                     VGKNLNVTLVEMGDRVLPIQLDKYASSKYEKRFEDAGIKLKLGVRAEKVLIDENKNPK
                     ALLINTGEEIPCELIIVATGVRSNVAFLKDSSIETDRFGLIINEKGETNARDVYGAGD
                     ITGRNPIWPTAVKEGIIAANNMVGNEIFMEDFFGSKNTMNFLGLTTMSLGIVNVPDDS
                     YTEEIDISGENYKKIIHKDGKIYGAIIQGDLSYAGVLTQLIKEKIHVSKVKKPLFEID
                     YADFFNIKENLEYTY"
     gene            complement(14334..14747)
                     /locus_tag="K8M77_000980"
     CDS             complement(14334..14747)
                     /locus_tag="K8M77_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI7085491.1"
                     /translation="MKRIKINRDKCVGCLTCTTACIVSHDSEDTRSRIAITSEGKYTP
                     IFCRHCDRPECVYTCMSGAMKKDKETGFVTYDKSRCASCFMCVMACPYGVLKAEHEMN
                     KYIMKCDMCASTKEKTPQCVAKCPMGAITLEEVEE"
     gene            complement(14760..16838)
                     /locus_tag="K8M77_000981"
     CDS             complement(14760..16838)
                     /locus_tag="K8M77_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HBI7085492.1"
                     /translation="MKRIQSTCNYCALACNLDFYTEDGKIKRVVPTPHYPVNKGFSCI
                     KGLNLDKQCTKFNGSKKPLLKMKDGERKAIEWKEAFDLFASKMTAIQEKYGKESVAYI
                     STGQLPTEEMALLGHVGRSYMGINGDGNTRLCMASAVVAYKQSFGFDAPPYTLKDLEL
                     SDTIFFIGANPVIAHPIAWGRVRKNKDAKIITIDPRKSETAMNSDMWIDIKTKGDLAL
                     FYTLANVLIEKGWIDQDYINNYTEGFEDFKAHVKKYTLEDVEERTGISKMRVLELAKI
                     IHEGKRVSFWWTMGVNQSYEAVRTAQAIINLALITGNMGREGTGANSLTGQCNAMGSR
                     MFSNTTALYGGGEYNNKERRKVVADILGMDENMLPSKPTLDYEQIIKGINKGEIKGLW
                     VICTNPRHSFSNNEEFKKAMKNLDFFVVQDIYEDTDSSKECDLYLPSVPAIKKEGFLI
                     NTERRLSAMVPVLEKEEDELSDYEIFLGIGEALGMGSLLDKWRTPKDAFNLLRECSKG
                     MPCDITGVTYERLRGSKGIQWPCREGEELEADERRLFEDGKYYTPSGKAKFIFEDVTE
                     NPNATNEEFPFNLNTGRGTVGQWHTHTRTREIQAVINIVPQKAYVDINRKDAERLDIK
                     ENDEVLIHSSNGHTSKFIARLTDNLKEKDLYAPIHYIETNLLTPSVFDPYSKEPSYKT
                     VQVNIEKVRK"
     gene            complement(17209..18486)
                     /locus_tag="K8M77_000982"
     CDS             complement(17209..18486)
                     /locus_tag="K8M77_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7085493.1"
                     /translation="MKVVVIGGGWSGVAAAIEAKKMGADVVLFEKTDLLLGLGNVGGI
                     MRNNGRYTASEELIAMGGGDLINITDKVSRHKDIPFPGHEHAWLYDVNLVEGEVKRYV
                     ESLGIETKMVSRIIDIKQEDNKILGVYTSDGQYYTGDVFIETTGSTGPMGNCLRYGNG
                     CSMCILRCPSFGPRISISSRCGVEDIQGERVGDELGAFSGSCKLAKETLSEEIRKELE
                     EKGCVVLKIPKEDVNYDKLNTKVCQQYALKEFAENVVLLDTGHAKLMTTYYPLEKLRR
                     IKGLENAKYVDPYAGGKGNSIRYLSVAPRDDNMKVKGVSNLFCAGEKSGLFVGHTEAI
                     VTGTLAGHNAVRHALGIPYLILPRATVLGDIIAFANEESQSREGKKNRYTFAGSVYFN
                     RMKELGLYTIDKDEIQKRVAQLNLDGVFSKKLM"
     gene            complement(18518..19498)
                     /locus_tag="K8M77_000983"
     CDS             complement(18518..19498)
                     /locus_tag="K8M77_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBI7085494.1"
                     /translation="MDYEVIDCIDAGTEFCPCHLAEEGECILCSQLHGKCFCDCVNWK
                     GVCIYEEFVSNGFKAKEDRKTFTCDVIDAVEVEEGLLFIEFRAPHKLCIDLLGPGKFI
                     FIRTNDNPFFDVPISILESDADKNIIKVLIEVRGIKTKRLLNTEVKGEITIRGPYFNG
                     VFGIKNIDSTKNGEVLVLCRGIGLAPAVPVIKKLANEGNKIKILLDKAPFKESYIEKY
                     LEGYDIQYVPMNLINKGEISNEAKEVIKNGQWDLIHCAGADILTYKLIEYLNDLKDES
                     TKVSCCNNAKMCCGEGVCGSCTARFAGHKVKRLCKVQSDPRKIFEDRRFI"
     gene            complement(19745..20620)
                     /locus_tag="K8M77_000984"
     CDS             complement(19745..20620)
                     /locus_tag="K8M77_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBI7085495.1"
                     /translation="MKKKISILMFFLITISICSNVIVLGKESCIPEEVSVETMDVKDA
                     YGKQCKDITPDAHFAIAYDAKNKKILYEKNAFRNVPMASTTKILTALVAINYSDLDEE
                     VTISKTAASIRGSKVGFRAGEKVTMRELLFGLMYKSGNDAAIAIAEHIGGSIENFSIL
                     MNDFARMLGLKDSNFQSPHGLDSQMHFSSAYDLAVLMSKAMEEPFFREISGTKEVGAE
                     KYNFTRSYSNINKMLWRLPNANGGKTGYTGQAGKCLVSSVDHNGRNIIIVVLNCPTRW
                     EATERVYEYVKANYS"
BASE COUNT         6497 a         4045 c         2057 g         8273 t
ORIGIN      
        1 ataatttaat ctaatactaa aatatcataa aaacatataa taatcccaat catcaaatag
       61 aactttaatc acaacagcta taggaactat aaaaaccatt cctaaaagcc ctcctatttg
      121 ctctccaatt aaaagtaata ttatgattaa tattggatgc atatctatac tctcagctgt
      181 aattttaggt gctattaagt ttccttcaat ttgttgaatt attaaaaaag ttaatataac
      241 ccataaaatt ttatttgggc cttctactag tgcaacaact attgcaggta ttgcccctaa
      301 aactgctcca aaatagggta tgatattgag taacgcattt aaaaatgcta ataatagagg
      361 aaactttatt cctagtataa gcatacctat aaaggtcata attccaacta ataagcttaa
      421 aagtaattgt cctaaaatat atttacctaa tactttatca acatctctcc ctagattttt
      481 taatagagct ctcttctcca ttgggcagca atataaaaat ttattagata ataaatctcc
      541 ataagctaaa aaataatatg ctaatacagg tataacagca aaagctaaaa tattttctga
      601 aaagctaatt aaattattta taatactagt tgcaaaacta actaataata tatttaattt
      661 ttcagtaact tgagcttcaa taacatcaaa aatccctaat tccataaact tcatcttcat
      721 aatcaagtca tttataaata attctacgcc atttattaaa ccatcaaaac tttccccttc
      781 tttaaacata cttggaatta gaactgttaa aaaaaataat ataagaaaaa ttacactaag
      841 cactatagac atagctaaaa agtttttatt tatttttgtt ctttcacata aaaatctata
      901 taaaggctta agaatatatg caaaaatccc tgatacaagc actataaaaa taatgtctct
      961 aaaagcttca aattgatata aaaggaatat aaataaaata attataaata ttaatattaa
     1021 cttttttata aatctttttt tatcttttaa aactctaaac attcctaatc ctcctttttt
     1081 aatttaccta gccaaaatat tttcttggaa tcattctcat atttatattt ttattgccaa
     1141 aaaataaaat actttcttct cctaaaatag tttcttttaa ttgtataact cgctttccat
     1201 ttttaaattt atcaactaat ccacttacca ctattagtcc tcttatataa aagtcgtagt
     1261 ctattaaaat atcttctaat attcctatca tatggccatc cttatttata agatcaaagt
     1321 tttttatctc gctaaatttt aatcctctat gtacatgtac cccttctgtc ataatagtat
     1381 cgcttaaaga aataacatta gttaagtcca cataatctct ttttgaaaat aatttttttg
     1441 ttattataaa gcccatgact ttcccattaa aataatctat agcaagatca caaactactc
     1501 caaccttctt tccattagaa tcaaaaacct taagcccaat taaatctctt attctataca
     1561 ctctaaaagt catccctttc taatttaagt aaagccatta gtaatatatt tagtattatc
     1621 tatatccttc caaaaaatac atagtttcat aggtatttta aatttatttt tgtaaactta
     1681 aataataatc cttaaacttt ctaaataagt tattaactaa ataaaataag tatatattct
     1741 taacttaata aatacacttt gtatagtgaa ttttatatta aaaataacct tttaaaaagt
     1801 tttaatataa aagaaaaaaa gccccctttc gggagctttc tattaaagta ggctttcgat
     1861 tattcctcca cctacaacta aatcatcttt atagaaaaca acagattgac ctttagttat
     1921 agctctttgc ttatcttcaa aagaaacttt aactcttcca ttttccatag gacttatagt
     1981 agccttagct ggtttagctg aatatctaat tttagcttca acttccatag ggtcttcaag
     2041 tttatctata gatatgaagt ttatatcttt acaaactaaa tcagttttaa agatatcctc
     2101 ttctggtcct actacaacag tattagttac agggtttata tcagtaacaa atactggtct
     2161 tccaagagct aatcctaagc cttttctttg tcctattgta taatagacta ttcctttatg
     2221 ttttcctaag acatttccat acttatcgac aaagtttcct gttctaactt tgtttggagc
     2281 agcttcacat atgtatcttc catgattatt atctggaata aagcatattt cttcactgtc
     2341 ctttttatta tgtacatcta gaccgatctc tttagctatt tctctaatct tatccttagt
     2401 gaattctcca caaggcatta aagtatgttc taactgttct tgagttagat tgtataaagc
     2461 ataagtttga tcttttctat catcctttga tcttataagt tggaatcttc catctctttc
     2521 ttcgatttta gcatagtgac ctgttgcaac atagtctgca cctatacctt tagctttttg
     2581 taaaagctca tcaaatttaa gatatttgtt acatgcaata catggatttg gagttctacc
     2641 ttgtatatat tcgtctacaa aataatctat tacgtttctt ttaaaactat ctctaaagtt
     2701 taaaacgtaa aaaggaatat ctagtttttg agcaactctt ctagcatcat ccactgctga
     2761 cagcgaacaa catcctcctt ctctttcctc atattcctta tcctcttgcc atatttgcat
     2821 tgtaacacca attacatcat atccctgttc ctttaaaagg taagcagcaa cggagctatc
     2881 aactccgcca ctcataccta taacaacctt tttctttgtc acaaaatcac cactttaata
     2941 ttattcttga ccgtgaacat gctcatgtat gtcatgtgaa tgttctttca tatcccaagg
     3001 ctctaatcct tgtctttgtc tatagtcatt tatagctttg tgtatagctt cctcagctaa
     3061 tactgaacag tgcattttta ctggtggaag accatctaaa gcttctgcaa cagctttgtt
     3121 tgataattcc caagcttctt ctacagtttt tccttttata agctctgtag ccatgcttga
     3181 tgaagctata gcagatccac atccataagt ttcaaattta acatcttcta caatgttatt
     3241 ttctatttta agatatattc tcattatatc tccacattta gcgttaccaa cttctccgat
     3301 tccgttagca tctttaattt ctcctacatt tcttgggttt ttgaaatgat ccataacttt
     3361 ttcactatat atcataatta attctctcct tctacttctc ttaaatgatt tttccataat
     3421 ggtgacatac ttcttaatct tgcgattatt ggtggtactt tttctaatac gtaatcaaca
     3481 tcttcttcag ttgtaccttc acctaaactt aatcttaatg atccatgtgc tatttcatga
     3541 ggtaaaccta ttgctaataa cacgtgtgat ggatctaatg atcctgatgt acaagcactt
     3601 ccacttgaac cacaaattcc ttcaaagtct aatgaaagaa gaattccttc accttcaata
     3661 aattcaaagc atacatttac gttacctggt aatctcttgc taccatcttt tggtccgttt
     3721 aatcttgtaa atggtacttc taataatcct tttattaact tatctcttaa cttagaaatt
     3781 ttagctatat gctcatctaa gttctctcca gctatttcca tagcctttcc taatccaact
     3841 attgcaggta tgttctcagt tccagctctt ctagctctct cttgaccacc accgtggatt
     3901 aagttatcta ttcttatacc ttttcttata tataaagctc cgattccttt tggtccataa
     3961 actttatgtc cagctaatga aagtaaatcg atgttcatat ctttaacatc tatctttaca
     4021 tttcctacag cttgaactgc atctgtatgg aatattactt ttctttctct acaaattttt
     4081 cctatttcct taactggctc tatagttcct atttcattgt ttgcaaacat tacagaaact
     4141 aagatagttt tatcagtaat agcatttttt aattcttcta aatcaacaaa accttcttca
     4201 tttacatcta agtaagttac ttcaaagcct tgtttttcta accattggca agtatgtaat
     4261 acagcatggt gttctatttt tgtagttatt atatgatttc ctttattttt atgagcaaaa
     4321 gctattcctt ttattgccca gttatctgct tctgatccac caccagtaaa gtagatttca
     4381 tttgtatcag catttaatac tttagctact ctttctctag cagtatctac agccattctt
     4441 gattctcttg aaactccgta aaatgctgat ggatttccaa acttatttgt gaaatatggt
     4501 aacatttcct ctaatacttc tggcttaaca tatgtagttg ctgagtagtc catataaaca
     4561 actctattat tcattttctt cactcctact aacaattttt aaagcttcat ttttttcttt
     4621 catagcttta taatcttcta ctatatcttt aagtgttact gaattagtaa cctcatctat
     4681 gcttgttttt atttttttcc ataaaagtct agtggcacaa caatcaacat tattgcaagt
     4741 tacaccatct aaacaatctg atatttctat cgggccttct aaaacctcta taatatctga
     4801 aactgttata tcttctggtt gtctatttaa aatatatcct ccttgagcac ctctaatact
     4861 ctttataagc ttagcttttc ttaaagaact aaataattgt tctaaataat attcagatat
     4921 attctgtctt tgcgatattg actttattga cacaggtgat ccaccataat ttatagctaa
     4981 atcaaccatg gcttttacac catatcttcc tttggtagat aatttcattt aatcacctct
     5041 aaatctgact aattcgatgt actttatgtg attataatat caaaaccgag taaatttgtc
     5101 aacaataata ttcacatatt tttcacttaa aaaatggaat tttaaattca aattcaacta
     5161 tttgtttttt acattattcc agtaggcttt tatgctactt tcgtatttat tatccttagg
     5221 cttataatat tcttttcctc tcatattttc aggcatatat tcttgattta tataactatt
     5281 aggataatta tgtggatata aatacccacc aacacctaaa ttgcttgctc cactataatg
     5341 agcatctttt aaatgttttg gcacatctcc cgaattattt ctttctaaat ctcttaaagc
     5401 atcatcaata gccatatacg cactatttga cttaggtaag gtagctaaat atattactaa
     5461 ttcagataat attatccttg cttctggcat tcctattttc aaagcaaggt ctattccgct
     5521 atttacaact tgcaaagcat taggatgagc aagccctata tcttcagctg caattactcc
     5581 tatcctccta attacgctat ccatattccc acccttaata agtctagcta aataatgaat
     5641 tgaggcatca gggtcacttc ctcttatact tttttgaaga gcacttaata aattatagta
     5701 atcatctcct gatgaatcag atttcaaatt agattgccct aatttttcta catactcttt
     5761 atttaaaagg atattatctt tttcttgaga tttaatagct aattctaata ttgtgtaagc
     5821 ttttcttaca tctccttgag aaatctctcc aatatacttt aaaacacctt cttcaagaga
     5881 aatcacatat ccttcttttt caagtttact tactgctctt tctaaagcta ttattatatc
     5941 ttcagtactt actggtttaa attgaaatat ggtacttctg cttagtatag ccttatgtat
     6001 agcaaagtat ggattttctg tagtacttgc tattaatatt actcttccat cttcaataaa
     6061 ttctaaaagt gcttgctgct gttttttatt aaaatgctga agctcatcta tatacagtat
     6121 tattccctta aaatttaaca aattatctat atcagaagtt atactttgta tttcttttat
     6181 ggaagatgta gtagcattta ttttataaaa tttcttatca gtgtaatttg aaattatatt
     6241 agctaaagta gtttttccaa caccaggtgg accataaaaa atgcaattta tcatgttctt
     6301 attttttata agattgctta aaggagttcc ttctcctaca atgtgtttct gtccaactat
     6361 atcctctaat tttttaggac gcataatatc tgctaaaggt ctcaaatttt tcaattatca
     6421 aaatctcaat aatattacta tgttaactga atacttctct taattttgta ttaattaaaa
     6481 agctccgtat ttataaaaca taatatatta atatgaaacc tgataatttt cctcccttct
     6541 tatactttta tagttaatga atttcctaaa gtaaatctta atttaattct aaaattcatt
     6601 ataatactaa aatacagttt tatttcttcc tgtcattttt gccatatata atttttcatc
     6661 ggcatttttt atggtatcaa aggtatcttc tttttctgaa atttcacaaa ctccaaagct
     6721 agaagttatt tttatactta aatcaccata gttaaagctc attttttcta tgatttttct
     6781 aagtttctct gcaaccttaa ctccattttc taaattggtg ttatttagaa caattatgaa
     6841 ttcttctcct ccgtatcttc caatccaatc attgttttct cttatattat cattcaacac
     6901 ttttgcaaaa tcccttaaga ttttgtcacc tactaaatga ccataagtat catttacttt
     6961 tttaaaggaa tctatatcag ccataattac agttgtaggt atagattctt ttaagtttct
     7021 attagattcc tttgaaaatt tttgatctat atacctttta ttatagactt cagttaagtg
     7081 atccttaaca agattttctc caatcttttt tattgttaaa tttaaataat tattatctcc
     7141 actaccatct actatactgc cattattagt tatatctttt aatatttcaa caacatattt
     7201 attttcatta aattcaattg gtgaagctat aactaaaaat acatggtttt tattacattc
     7261 taactttata aaagtatcat tttttaaata agcttttttt gatatacaag tgctacatgc
     7321 tgaattttta ccccatatat cataacactt acttaaggaa caatcttttt cattaaattc
     7381 attatttatt tttaggttaa agtcatctga aaatactata ttatttatag gatctactac
     7441 tctaacttca tcataaaagt tctttaatat agacattctt tcttttacat ctagtaaaac
     7501 ttccaatatt ctttcctcca gtccttagaa cttttaattt ctttctacac cttactacat
     7561 attaaaggaa tatctaaaac taattattct taaatttata cctctctatt ctccgtacat
     7621 tctttattaa caattataca ataaaaaaag attctaataa aaactattag aacctttata
     7681 ttaaaaaatt tttaaagaag ttctacctct tctcctaaaa ttacttctcc tccattcaat
     7741 accttggtaa atatgccata agcaggcatt atgcatcttc ccacttgata atatattgca
     7801 catttatcat gacatttttt ccctatttgt gttacttcta aaagaacctc tcctatcttt
     7861 attttttgcc ctataggaag tttgtttagt tctattccat ctacaaccag gttttctcca
     7921 aaggctccaa aatccacatt tccgccatca gctctaaaat cctctatctt ttcaaaagct
     7981 aataaactta cttgtctatg ccacttccca gcatgagcat cacccttaat tccgaaatct
     8041 tctataaact cagccttagg aactttaacc ttagctgtac cttttttttc acttatacaa
     8101 atagctctta ccttgctcat ttttatcctc ctatttcata cataaatctt ttatcttcat
     8161 ctttttcttt cattttaaag ttatgttttt ctggcttttc ataaatactt tttttaatta
     8221 tctccctaag ctcttcatca cttattccat ttctcatttt atctcttaag tttattccat
     8281 acttccaatg taaacactgt ttcataaagc cttcaggagt gacccttatt ctattacaat
     8341 cctcacaaaa acaatcactc atagcactta taaagcctat ctttcctttg aatccttcta
     8401 tgcttatgta tttagctgga ccatttagtc ttaaggtttt tccatcactt aatacctttt
     8461 tttctttctt tataatttct aaaatctcac tattagtaac gcccttaaac tttttacctt
     8521 ctccaatagg cataagctct ataaatctaa gatctattgg atttttataa gctaagttta
     8581 ttaaatctaa aatttcatct ttattgaaat cttctaatat tacagtattt atctttacct
     8641 taacattatt ctctaagcac ttttctattg aataaagtac tttatctaag gttcctatcc
     8701 ttgttatttc tttaaatcta tcttctttta gtgtatctaa acttatattt actctcttta
     8761 atccattttc actaagttcc tttactttat ctcctagtaa aatcccatta gtggttaaac
     8821 aaatctcttc tatctctggt attttattta tattttttat taaatctact attccttctc
     8881 ttacaagagg ctcaccacct gtaagtctta ctttttttat tccaatagaa gcacattctt
     8941 tgacaactct caaaatctca tctaaagtca taagcttatc attgtgaata aaatcattgt
     9001 gatctttctc catgcaataa gcacatctta aattacattt atcagttaaa gaaattctta
     9061 aataatcaat ttctcttcca tatttatctt tcattttctc accttcttct ttatacaaat
     9121 tcaattccct cttttaggtg aataatgatt ttatctcttt atccaaactt ttttaatctt
     9181 taataacaat tttaatctaa tatatattat aatttttatc tattgaccta ataatctcct
     9241 tactttccaa aggaacaatg tggaaaagta caaactgggc aatttaaaca aagtccacca
     9301 tgaccatatt cagctatatc tcttaaagtt aatctttcat ctgctaaaac tcttggaagg
     9361 actaaatcta aagctgttct ttttgaatac atggcacagc ttggtattcc aataatagga
     9421 acatcattat aataagctaa aagaagcata gctcctggaa gtacaggtga accatatgtt
     9481 attaattctc ctccacattc ctttatagcc gtaggagtaa catcatctgg atcaactgac
     9541 attccccctg tacaaagtat aagttccgca ccttcatcta aagctctttt tatttcgtct
     9601 ataattttct ccttatcatc tggaagaata acctgtcttt caagatctat attaaattcc
     9661 ttaagctttt ccttgagaac aggaccaaag gcatctttta ttcttccact ataaacttca
     9721 tttcctgtag ttattgcaaa agtcttttta ttttttatct ctttaacttc tataatctta
     9781 tcttttataa gattttctgc ttctattatt ttattttcat ctataactag tggaattact
     9841 ctagtagccc ctaccttatc acctttttta actggaaaat tcccatgaat tgtagaaact
     9901 attatttcac ctagcatatt taattctaat aacttttcct tatctatttt taaaagtccc
     9961 tctttatcag ctaaaaattc tatttttcct tcttttattt ctccaaaact taatccttct
    10021 ccacaaacta gatcctttaa tctttcagca gcctcatttt catgaagcat tccctcttct
    10081 ttttcccata cataaacatg ttcttttcct attgataaaa gtttttctat atcttcttct
    10141 cttataacat ggcccttttt aaaggctctt cctttaaact tacctggtat tatttgagtt
    10201 acatcatgag ataatattgt tccaacggca tcttcaactc ttatttgttt cataataatc
    10261 ctgttataag ttatttattt taaatctctc tccatctaaa actatatact ttaatataaa
    10321 taactttaaa cagttcacct cctcctagta tttttagtaa taaaagattt cctaaatctc
    10381 ttttttcctt attaaatctt catattcatg aaccgtattt atattataaa aattctttat
    10441 ttctttttta tgtatttttt ctataaaagg aaattcttca actaccttca tagaaaattt
    10501 aaaacaagtt tttattaatt tattgctatt atttataagt tctttttcca taatatcttt
    10561 gcaacttttt ttatatatgg cacaaagagg ttgtagtctt tcctgaaatt taggaattaa
    10621 aatttcataa tcttccttta tatttcctaa atagttaacc acttctttat ttataaaagg
    10681 catatcacag gcaacaacta agcaataatc atttttaata taatttaagg ctgagtgaat
    10741 tccacttaaa ggcccttggc taggatatat atccttaaaa actttaagtc caaactccgt
    10801 gtactcttct ggattattag atattattat tatttcctca taatcttcta aggcttttat
    10861 tattctctct ataaaatagt tctcttcata ttttaaaaaa gccttattta tataattcat
    10921 tctactactt ttccctcctg ctaaaatagc agcactcttt tttatcacct taatacacct
    10981 cctacaaact tatttttaag ctaattattt attataattt tattatgctt actacttctc
    11041 ctctatttaa gccttcattt cctttttcaa tttctattaa gcaattagca tttagaagag
    11101 aacttaatat accatttcca cttttaattt gagttatata aacctttgct cccttttcat
    11161 catatataac attccctctt aaaaaccttc tttgaggact ttttttatta aaagaatcca
    11221 ttaagactcc cttttctctt ttaatattta tattttcttc ctctccctct aattttttaa
    11281 gtacagactt tcctaataat tcaaatgtag ttaaggcagc attaggattt cctgaaagac
    11341 ttattaatat tttttcttcc tttttgctac atacaactgc agagccaggt ttaattttta
    11401 ttttccaaaa taacttttca aagcctatac tttcagaaac cttttgcata agatcctttt
    11461 ctcctacaga agctcctcct gtagtaaata ctatatctga tttttcaaag gctttttcta
    11521 tgtcttctcc tatttttagg atattgtctc ctacatgttc catagacaaa acattgtatc
    11581 ctagctcttc aattcttgca cttaaactat ataaattact gtcatatatt tttcctggtt
    11641 ttaaggtgga atttatatca catacctcat cacctgtgct aattattgaa actcttggct
    11701 ttttataaac aagtacctca cttataccgg agctagccat tatacctaaa tcagcataat
    11761 caagtctttt acctttttta attaaagttt ctccaatctt tatatcctct cccataaaag
    11821 atatattctc ataagatttc atttccttaa atattttaac caaatctcca tgtctttctg
    11881 tttcttcttg cttaagtacg caatcagctc ccacagggat tggagcccca gtcataattc
    11941 taacagcttc tccatgtttt atttctattt ctggccacat ccctgcaaat atctcatcta
    12001 ctattttaag ctctatagga ttttcttttg aagttccctt agaatcttct gatttaaaag
    12061 tatatccatc aaaaactgac ttattaaagg gaggattatt aatcttagat ttaacatcac
    12121 catacaaaac tctctttaaa gaatccttta tgctaacaac ctcacttttt aaagccttag
    12181 tgttcttatt taatatttca agagcttctt ctaaagctat aaattttttc aaaatatcac
    12241 catcccttta taaaataaaa tgcttattat ttttgttata ccaacaagaa taataagcat
    12301 taaattaaat agtcctttta gaccttaata ttaaaactaa aaaacataat taacaatatt
    12361 attatgtaag ttctatgttt tttacttttg cttattctcc ttcccaaaat acgtggaggc
    12421 aaaattcttt caaaccttac cctgtggatt accaacttaa tgttaaataa tttagtaatc
    12481 caggcttaaa aatcatagaa ccttctcctt agatctttaa gcttggagag ttatactaat
    12541 tttctattac aaggttagca atttcttcat aattatttaa gtcaacaact ctaatccctt
    12601 ctagagataa actaacatca ctagcaattc ctataagctt ttcctttgga gttattattt
    12661 tcttactaat accttgtcta tatacttcaa gctttctata tttgctattt ttataacctt
    12721 ctattattat aaaatcgaaa tcttcaactt gctttattaa ctcttctaga ggaacttctt
    12781 cctgaacctc tcttataaga gccattcttt cctttgagga aattattact gcttcagctc
    12841 ctgcttttct atgacgccaa gtatcttttc cctctttgtc tatatcaaag ccatgaacat
    12901 catgctttat agtagcaaca gtatatcctc tatttttaag ctcttttata agcccttcta
    12961 ttaaaactgt ttttcctgta ttagaacagc tagcaactac attcacaatc ttagccaaag
    13021 ccttttcctc ctaattataa ctttaattta ttattttcta aattaaaatt tacatactta
    13081 tattaaataa atttaattat aatttatttt aatatgtgta ctctaaattt tcttttatat
    13141 tgaagaaatc tgcatagtca atttcaaata atggtttttt aacctttgat acgtgtatct
    13201 tctcttttat aagttgagtt aaaactcctg cataagataa atcaccttga attatagctc
    13261 cataaatttt tccatcctta tggattatct ttttataatt ctctcctgaa atatcaattt
    13321 cttctgtata agaatcatct ggaacattaa caatgcctaa agacattgtt gtaagtccta
    13381 agaaattcat tgtattttta cttccaaaga aatcttccat gaatatttca ttaccaacca
    13441 tattgtttgc tgctattata ccttctttta cagcagttgg ccatatagga tttcttccag
    13501 ttatatctcc agctccataa acatctcttg cattagtttc gcctttttca tttattatta
    13561 atccaaatct atctgtttct atagaactat cttttaagaa tgctacattt gatctaactc
    13621 cagttgcaac tattataagt tcgcaaggaa tttcttctcc tgtatttata agtaatgcct
    13681 ttggattttt attttcatca attaaaactt tttccgctct aactccaagt tttaatttaa
    13741 ttccagcatc ttcaaatctc ttttcatatt tagaagaagc atatttatca agttgaattg
    13801 gaagaactct atcccccatt tcaactaaag taacatttaa attttttcca acaagtcctg
    13861 ctatggcgtc tataccaaca agtcctgctc ctaagacaac cacgttttta accttttctg
    13921 cctcttcttt aattttaata gcatcctcta agtttcttaa tccaacaaca tttttagctt
    13981 ctcttaaatt ctctactgga ggaatgaatg ctgatgctcc tgttgctaat aatatcttat
    14041 catattttaa gctttctcca tttgaaagac ctattacatg ctctctatca tctatagatt
    14101 taacttctaa accttttttc cactctatat tatatttttc aaagaaatct ctatctgtaa
    14161 aatctaaagc ctcgatatct ctatgaacac ttatgtaatg atgtaatata catcttgaat
    14221 aaacattttc gtcctttgaa actaatatta tttctgcatc tttgtcaagt tctctaagtg
    14281 ttttggctcc acttattcca gcagctgatg cccccacaac aatatatctc atattattct
    14341 tctacctcct ctaaagttat tgctcccatt ggacattttg caacgcattg aggagttttt
    14401 tcttttgtac ttgcacacat atcacatttc attatatatt tattcatttc atgttcagct
    14461 tttaatactc catatgggca agccattaca cacataaaac aacttgcaca tcttgatttg
    14521 tcataagtta caaaaccagt ttctttatct ttcttcatag caccactcat acaagtgtaa
    14581 acacactctg gtctatcaca gtgtctacag aaaataggag tgtattttcc ttcacttgtt
    14641 attgcaattc tacttcttgt atcctctgaa tcatgagaaa ctatacaagc agttgtacat
    14701 gttaagcatc ccacacattt atctctattg atttttattc tcttcaaagt aattcccccc
    14761 tattttctaa ccttttctat attaacttga actgttttat atgaaggctc cttagaataa
    14821 ggatcaaata cagatggtgt taataaattt gtttctatat agtgtattgg tgcatataaa
    14881 tctttttctt taagattatc agttagtctt gctataaact tagatgtatg cccatttgat
    14941 gaatgaatta aaacttcatc attttctttt atatcaagtc tttctgcatc ttttctattt
    15001 atatctacat aagccttttg tgggactata ttaattacag cttgtatttc tctagttcta
    15061 gtatgagtat gccattgacc tacagttcct ctaccagtat ttaaattaaa tggaaactct
    15121 tcgtttgtag catttggatt ttcagttaca tcttcaaaga taaacttagc ttttccactt
    15181 ggagtatagt attttccgtc ttcaaataat cttctttcat cagcctctaa ttcttctcct
    15241 tctctacaag gccattgaat tcccttagaa cctcttaatc tctcataagt tactcctgta
    15301 atatcacaag gcattccttt actacattct cttaaaagat taaacgcatc ctttggagtt
    15361 ctccatttgt ctaaaagact tcccattcct aaggcttctc caattcctaa gaatatttca
    15421 taatcactta attcatcttc ttccttctct aaaacaggaa ccatagctga aagtctacgc
    15481 tcagtgttta ttaagaaacc ttctttttta atagctggaa ctgaaggtaa atataaatca
    15541 cattctttag aactatctgt atcttcataa atatcttgaa ctacaaagaa atctaggttt
    15601 ttcatagctt ttttaaactc ttcgttgtta ctaaatgaat gtctaggatt agtacagatt
    15661 acccataatc ctttgatttc tcctttattt attcctttta ttatttgttc ataatctaaa
    15721 gtaggcttac ttggaagcat attttcatcc atgcctaata tatcagcaac tacttttctt
    15781 ctctctttgt tattgtattc tccaccacca taaagagcag ttgtgttact aaacattctt
    15841 gatcccatgg cattgcattg tcctgttaaa gagttagctc ctgttccttc tcttcccata
    15901 tttcctgtga ttaaagcaag atttataatg gcttgagcag ttctaacagc ttcatagctt
    15961 tggttaactc ccattgtcca ccaaaatgaa actctctttc cttcatggat tatttttgca
    16021 agttctagaa ctctcatctt agagattcct gttctttctt caacatcttc taatgtatat
    16081 ttcttaacat gtgctttgaa atcttcaaag ccctctgtgt aattatttat ataatcttgg
    16141 tctatccatc ctttttctat aagaacattt gctaaagtat agaaaagagc taaatctccc
    16201 ttagttttta tatcaatcca catatctgaa ttcatagctg tctcagattt tcttggatct
    16261 atagtaatta ttttagcatc cttatttttt ctaactcttc cccaagctat tggatgagct
    16321 ataactggat ttgctccaat aaaaaatata gtatctgaaa gctctaaatc ttttaaagta
    16381 tatggaggtg cgtcaaatcc aaagctttgt ttgtaagcta caactgctga tgccatacaa
    16441 agtcttgtat taccatctcc atttataccc atgtagcttc ttcctacatg acctaaaaga
    16501 gccatttcct ctgttggtaa ttgacctgta cttatgtaag ctacactttc tttaccatac
    16561 ttttcttgaa tagctgtcat cttactagca aataaatcaa aagcttcttt ccattctata
    16621 gcttttcttt ctccatcttt cattttaagt aaaggcttct ttgaaccatt aaacttagta
    16681 cattgtttat ctaaattaag tccttttata caagaaaatc ccttattaac tggataatgt
    16741 ggagttggaa ctactctttt tatctttcca tcctctgtgt aaaaatctaa attacacgca
    16801 agagcacaat agttacaagt agattgtatt cttttcatac ttttttcctc cgttcacaaa
    16861 cataactttg ttaaaaattt atcatactat ttttgattat atcattatgt tatccataat
    16921 aatatgataa ttatcacgtt tttcaattat attttaaatt atctttattt ttatttaaac
    16981 cctaattaaa tgtgtaataa aataaccttt tctaaattac ttaattttat aatataaatt
    17041 ttaattcaaa ttatattcct acctaattta aaactctatt taattaatta aaacctctta
    17101 atttatttat aaaaataaag tatctaaaga atataaataa acttaatatt tatattctaa
    17161 agatactttt tgtgttcaaa ctatatttta aacataataa tcttaaattt acataagttt
    17221 tttgctaaaa actccatcta gatttaattg agctactctc ttttgaattt catctttatc
    17281 tattgtataa agtcctaact ctttcattct attaaagtaa actgaacctg caaaggtata
    17341 tctatttttc tttccttctc tagattgaga ttcttcatta gcaaaggcta ttatatctcc
    17401 taaaacagtt gctcttggaa gaatcaagta aggaattcct aaagcatgtc ttactgcatt
    17461 gtgtccagct agagtacctg taactatagc ctctgtatgc cctacaaaaa gccctgattt
    17521 ttctcctgca cagaataaat tactaactcc tttaaccttc atattatcat ctcttggagc
    17581 cactgatagg tatcttatag agttaccctt tccacctgca tatggatcaa catacttagc
    17641 attttcaaga cctttaatcc ttctaagctt ttcaagagga taataagtag tcataagctt
    17701 agcatgtcca gtatctaata aaacaacatt ttctgcaaac tccttaagag catattgctg
    17761 acaaaccttt gtgtttaatt tatcataatt aacatcttct tttggaattt taagaactac
    17821 acatcctttt tcctcaagct cttttcttat ttcctctgaa agagtttctt ttgcaagttt
    17881 acatgaacca ctaaatgctc ctagttcatc tccaactctt tctccttgta tatcttcaac
    17941 tccgcatctt gaacttatac taattctagg accaaaagaa ggacatctta aaatacacat
    18001 agagcatcca ttaccgtatc ttaggcaatt tcccatggga cctgttgaac cagtagtttc
    18061 aatgaacaca tctcctgtat aatattgtcc atcacttgta taaactccta aaattttatt
    18121 gtcttcttgt tttatatcaa tgattctaga aaccattttt gtttcaattc ctagactttc
    18181 tacatatctt ttaacttctc cctcaactaa gttaacatca taaagccaag catgttcatg
    18241 tcctggaaat ggtatatcct tgtgtctact aaccttatca gttatattta ttaaatctcc
    18301 acctcccata gctattaatt cctcagaggc tgtatatctt ccattatttc tcattattcc
    18361 gccaacatta ccaagtccta ataataaatc tgtcttttca aaaagaacta catctgcccc
    18421 catttttttt gcttcaatag cggctgcaac tcctgaccat ccaccaccaa taacaacaac
    18481 cttcataaaa actctccctt ctataaatta atctttatta aatgaatctt ctatcttcaa
    18541 atatttttct aggatcactt tgcaccttac aaagtctttt tactttgtga ccagcaaacc
    18601 tagctgtaca gcttccacat accccttctc cacagcacat tttagcatta ttacagcaag
    18661 agacttttgt agattcatct ttcaaatcat ttaaatattc tataagttta taagttaata
    18721 tatccgctcc agcacaatgg attaaatccc attgtccatt tttaataact tcctttgctt
    18781 catttgatat ttcacctttg ttgattaaat tcataggaac atactgaata tcatatcctt
    18841 ctaaatattt ttctatataa ctttctttaa aaggtgcctt atctaaaaga attttaatct
    18901 tatttccttc attagctaat tttttaataa ctggtacagc aggagctaat ccaattcctc
    18961 tgcaaagtac aaggacctct ccattcttag ttgagtctat attttttatt ccaaatactc
    19021 cattaaaata aggccctctt atagttattt ctcctttaac ctcagtattt aagagcctct
    19081 tagtttttat tcctctaact tcaataagta cttttattat atttttatct gcatcagatt
    19141 ctaaaataga tataggtaca tcgaaaaatg gattatcatt agttcttatg aatataaact
    19201 tgcctggtcc aagtaaatca atacaaagtt tatgaggtgc cctaaactct ataaataata
    19261 atccttcttc aacttctacg gcatctatga catcacaagt aaaagttttt ctgtcttcct
    19321 tagctttaaa tccattactt acaaattctt catatatgca aacccctttc cagtttacac
    19381 aatcacaaaa acactttccg tgaagttgag aacaaagtat gcattctcct tcttctgcta
    19441 agtgacaagg acaaaattcc gtaccagcat ctatacaatc aattacttca taatccataa
    19501 aaaccctccc atttgaattc ttactattaa aatatccctg agagattaaa aatgttacaa
    19561 aatttcttta ataaataatt aatccttaaa atattttcta aaattatttt aaagatagaa
    19621 ctttaaatcc ctggtattaa aagctattaa gcatttatat ataacaaaat aatatatttt
    19681 ttaaacttta aaacaaaaaa aatcaaaggt gttatattaa aatatcaata taacaccttt
    19741 gattttaaga ataatttgct ttaacatatt cataaactct ttcagtggct tcccacctag
    19801 ttggacaatt taaaactact atgattatat ttcttccatt atgatctaca gaacttacta
    19861 aacatttccc tgcctgacca gtataacctg tttttcctcc attggcatta ggtaatctcc
    19921 aaagcatttt atttatattg ctataacttc tagtaaaatt atatttttca gcccctacct
    19981 ccttagttcc tgatatttct ctaaagaaag gctcctccat agcttttgac attaaaaccg
    20041 ctaaatcata agcagaggaa aaatgcattt ggctatctaa tccatgagga gattgaaagt
    20101 ttgaatcctt aagacctaac attctagcaa agtcattcat aagtatagaa aagttctcta
    20161 tactacctcc aatatgctct gcaatagcta tggcagcatc atttcctgac ttatacataa
    20221 gaccaaaaag aagttctctc attgtaacct tttctccagc cctaaatcca acttttgacc
    20281 ctctaatact tgctgctgtt ttagatattg taacctcttc atctaagtca gaataattta
    20341 tggcaactaa agcagttaat attttagtgg tacttgccat aggtacattt ctaaaagcat
    20401 tcttttcata taaaatcttt ttatttttag catcataggc aatagcaaaa tgtgcatcag
    20461 gagtaatatc cttgcactgc tttccatatg catcttttac atccatagtc tctactgaaa
    20521 cttcctctgg aatacaactt tcctttccaa gaacaataac attagaacat atagatattg
    20581 ttattaaaaa aaacataaga attgaaatct tctttttcat aaaatatctc tccttaaact
    20641 aagctttttt atctatgaaa ccttctttta ttagctttcc actttataaa tttaataacc
    20701 tagtatattt ttggataatt aaagctatta ttgtcaataa tgtataaatg gtaaggaggt
    20761 ttatgatgtg cttaagtttt ttttattatt actactttta gttttattgt tttttccaat
    20821 aaatttaaaa ttcaaattag tattttcaga aaaagatttt aagattaatt tt
//
LOCUS       DAEVGI010000030        19053 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEVGI010000030 DAEVGI010000000
VERSION     DAEVGI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 19053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19053
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.030"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            102..1880
                     /gene="recQ"
                     /locus_tag="K8M77_000985"
     CDS             102..1880
                     /gene="recQ"
                     /locus_tag="K8M77_000985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBI7085496.1"
                     /translation="MRREFEVLNNIFGYREFRKGQQEVIDKILSGKDVFCILPTGGGK
                     SLCYEIPAYIFKGTTLVISPLISLMKDQVDNLNSLGINAAYISGGNDFEEVKNIIRKF
                     IKGEFRLLYVSPERLENRYFLDNIEEANIAQIVVDEAHCVSMWGHDFRKSYSKIKPFI
                     CNLKKRPIITAFTATATETVMKDSIELLGLHNPFIYKGSFSRDNLEINVLKEVDKLEI
                     ISEIISEHEEESGIIYCSTKNEVEELYKHMLYRGKSVGKYHGSLKDKEKNYYQEEFLN
                     DNFKVMIATNAFGMGIDKPDVKFIIHSTFPKSIENYYQEIGRGGRDGSLAKCYLLYSE
                     QDIRVMDYLISSTTEISRRTIELKKLEKIIEFCNYDKCLRKYILDYFGEENSIKYCNN
                     CTNCLKNSDLIDMTLEAQKILSCIYRTKEAFGESVLIDILRGIHGPKVEKYKLYELST
                     FGIMKEYTSKYIKDIIKELLSIKALERKEGTYSMVKLNRKSIGILKGKEKVFLDVNNN
                     EESMCMDLELFKKLRILRKDISRREGVKPYIVFTDSMIMEIINKNPKSKEDLKNIRGF
                     GEQKITKYGPFILTTLRDYEKYGKRN"
     gene            1971..3893
                     /locus_tag="K8M77_000986"
     CDS             1971..3893
                     /locus_tag="K8M77_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HBI7085497.1"
                     /translation="MDFKEFIKSDREKHNKQKFKGTFLDYLEIVKNNPHVAKLSHKRI
                     YDLIMDKGFEILRPEENAKIKKIYGNEKIKKYNFFKEDFYGIDTVIMKLMNYFHSASM
                     RGEEARQVLYLVGPVGAGKSSLVESIKRVLETAPPVYVLDGCPMHEEPLHLIPKHLRK
                     EFEDMLGVEIEGDLCPVCKYRLLNEFGGEYEKFPVSTKNFSIRSRKGIGVVPPVDPNN
                     QDTSILTGSIDISKMDMYPEDDPRIFSLNGAFNVGNRGLVEFIEVFKNDVEYLHTIIT
                     ATQEKSIPSPGKGSMIYFDGLIIAHSNEAEWNKFKSDHTNEAILDRIVKIEVPYCLEL
                     SEEIKIYEKILKKSNFDAHITPHTMEIASMFAILTRLLPSMKVDPITKLKLYNGEEIV
                     EKGTTKRIDVLELKEEAGTMEGMKGISTRFIIKAIDNALSNAEHKCINPLSVMESIIK
                     SVKDMDISQEDKKKYLGYIQDTIRKEYNKILEKEITKAFIHSFKEQAESLFNNYIDNA
                     EAYVNKTKLKDSSTSEELEPDEEFMRSIEEQIGISESSSKGFRADVTSYMFYIVRSGG
                     KMDYRSYEPLKEAIEKKLTASVKDLSRIITKSRVRDKDQDRKYNSMVNQMEKNGYCPH
                     CCDVILKYAANNLWKD"
     gene            3897..5075
                     /gene="yhbH"
                     /locus_tag="K8M77_000987"
     CDS             3897..5075
                     /gene="yhbH"
                     /locus_tag="K8M77_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HBI7085498.1"
                     /translation="MAIFRDQAENHVEHDRSIEDRRRHRQLVEKSIKENLGDILSEES
                     IIGETKNKKYKIPIRGIKEYQFIYGANNKGVTTGTGEESRGDRISSDKRKAISNNKAG
                     NQEGKDIYETEITLEELMDYIVEDLDLPNLDKKKYSEIIVESAAKKRGYQKYGVRPRL
                     AKKKTVMCKIARKQGKKRALREIGEEAKIGRFPFREDDLRYYKVKKHPKKESNAVMIF
                     IMDVSGSMDNTKKYLARSFFFVLSRFIRRKYNNVAFEFISHTTTAKNVNEYEFFHKGE
                     SGGTYISSGINAAIDLIKEKYNPGVWNIYPFYASDGDNWSEDNEKAMEAVNEISDLSN
                     MFGYIELLPSTYSTTMFYRFKKEISKENFVSVTVKEKKDLWNAIKYMLSEELQEKNKE
                     "
     gene            5102..6451
                     /locus_tag="K8M77_000988"
     CDS             5102..6451
                     /locus_tag="K8M77_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBI7085499.1"
                     /translation="MDYNLKDLEGWNNKIEEIAKSEGLDYYPQEYEIIGYNEMLSYEA
                     YVGMPSKYPHWSYGKAYEKNKTLYSLNMTGLPYEMVINSNPCLAYLMKDNTLLLQILT
                     MAHVYGHNDFFKNNRLFKKGTDANNALEMFKLDADTIRGYINDPSIGYSQVERILDSA
                     HSIRYQVPRVIGEKRESQDEQRKRVLEIFNKKKENRGILDSMEEILPPDISKIPLNPD
                     DDIIQFIIDYSNLEEWEKNILKIVRRESLYFLPQIETKIMNEGWASYWHYNILKKLNL
                     PQGLHLEFLKRHNDVVAPVSYGLNPYYIGFRIFQDIEKRYGKEKIFEVRALERDNSFI
                     RKYLTRELCEELNLFQYVKKGFDYVVEEVSDDLGWKSIRNLIADECGVSSIPHIRVRE
                     LNKKDMTLNLEHVFDGRELEANYAKKTLKYINILWGRKVNLSSKGKDGSDILFMCDE"
     gene            6503..7039
                     /locus_tag="K8M77_000989"
     CDS             6503..7039
                     /locus_tag="K8M77_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBI7085500.1"
                     /translation="MKSLKGSQTEKNLYKTFAGECRARTKYNLFAEEAREEEQHWIAD
                     IFDETAKNEYAHAREVFRRFLGNVGDILDNLMVAAMGESDEFKEIYKEFEETARSEGY
                     EEIADFFKELREVEESHNERYMDFYDKVKSKSLYSSKEPVKWVCTNCGYIHEGKEAPM
                     NCPLCRFPQGYFRLLNEC"
     gene            7061..7411
                     /locus_tag="K8M77_000990"
     CDS             7061..7411
                     /locus_tag="K8M77_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085501.1"
                     /translation="MGQIEIFRKHFKEVNNLSVVLSSYANTYRLLVGAAGELNNISKV
                     RKRDLDDALDRVDEMGKIIDSILEVINDNEEAYIKYIKLKSKFIMEKASKDIIQTEVE
                     QDLLFENSNREEEE"
     gene            complement(7457..7978)
                     /gene="pssA"
                     /locus_tag="K8M77_000991"
     CDS             complement(7457..7978)
                     /gene="pssA"
                     /locus_tag="K8M77_000991"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBI7085502.1"
                     /translation="MRKSSIPNIFTFINLSFGILSLLATFKSEYLLAAIFILLAGLVD
                     RYDGRVARYLNVSSDLGKELDSLADLVSFGVAPSVLTFVFYNFESLGPMGIIGYALLL
                     CFPICGAFRLARYNATEFDGVFRGVPITIAGAVVALFILLSIKFNLNILFPVVILVIF
                     SYLMVSNLQLKKF"
     gene            8298..9179
                     /locus_tag="K8M77_000992"
     CDS             8298..9179
                     /locus_tag="K8M77_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBI7085503.1"
                     /translation="MKKVRFIYNPYSGENAILKELDKIITMHQDKGYTIVPYRIQRGV
                     DVINALDIIDSTYEYILIAGGDGTIDTVVNAMKKRGIEIPIGIIPVGTANDFAKHLGM
                     PQDAGACVEQILDSYAVGVDLGKVNDNYFVNVLSTGLFTDVSQKTDTNLKNTIGKLAY
                     YLKGLEELPNFRKLDVKITANGKVFNEPIYLMLVFNGQTAGNFKLATKAKVQDGLLDI
                     VIFKACHIPELIPMFIKVLKGEHLEDSRIEYIRTDELLIESDEEIVTDIDGERGPDFP
                     LRVQCIKDGIQVLGINK"
     gene            9252..9932
                     /locus_tag="K8M77_000993"
     CDS             9252..9932
                     /locus_tag="K8M77_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085504.1"
                     /translation="MNQTFIYYTVLFLIIFITISNLVENYRYSPKKIKNIIGIAFLLQ
                     IIRIVSLIALALVSDQRYISLFKYVGFLDIIYIPLMALIAFYILLRSDKINFQFFKFA
                     PIVLTVFYIGVITIFKPFFKMSWEYGYVITTEKQILLKSIYILLLVSILSFLILFKKE
                     NHSDKKGIVFLGVTLAFIIIENSLELLGVKFLNGAIISELILCILGFYIVLTFKKNRV
                     SIRNCIKS"
     gene            complement(10113..10787)
                     /locus_tag="K8M77_000994"
     CDS             complement(10113..10787)
                     /locus_tag="K8M77_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI7085505.1"
                     /translation="MIKIKNLEKSFKGNKILENISFTLKEGEILTLLGPSGAGKTTIL
                     RILNGLETCDFGDITLDSLKICEKGIYKDFKAQKEIHKKIGLVFQTFNLFPHMNILEN
                     LILAPMHTLNLSLEEATKKALQILSKLSLEDKAKSYPCELSGGQKQRVAISRALMLNP
                     KYICFDEPTSALDPSLTKDVSNIIKSLAKENIGVLIITHDMEFAKEVSSRIIKLDSGK
                     ILDDIS"
     gene            complement(10799..11440)
                     /locus_tag="K8M77_000995"
     CDS             complement(10799..11440)
                     /locus_tag="K8M77_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI7085506.1"
                     /translation="MNYIITLMPSIIEGLKNTLGVFILTLLLSIPLGMIVAILRLSNI
                     KIVNFISSLYVLVMRGTPLLLQLIFIFFGLPIIGISIDRFPAAIIAFTLNYAAYFGEI
                     FRSGIKDIDKGQFEASKILGLSKWFTFKKIILPQSFKKTLPSITNEVITLVKDTSLVY
                     VVGIGELLRAGKIASNRDASLLPLFLIGIIYLLLIGILTKIFNRLEKKYSYYN"
     gene            complement(11454..12242)
                     /locus_tag="K8M77_000996"
     CDS             complement(11454..12242)
                     /locus_tag="K8M77_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7085507.1"
                     /translation="MKKLLFLLIGIVILSILIFGCSSKKIETKKDSNKVIIGMDETFV
                     PMGFKNENGDIVGFDVDLAKEAFKRMNLKVIFQPIDWSMKETELTNGNIDLIWNGYTM
                     TPEREKKVAFTNSYLKNRQVIVTLSNSNINTLKDLKGKTVTTQDGSSSLDTLFENPEL
                     TKSFKNGEPVLFDSFNDCFMDLEAGRSDAVVCDEILAQYYIFKNDPSKFHVLTEDLGE
                     ESYSIGLRKNSNLIDPLNKTLEDMKEDGTIAKISNKWFGKDLEK"
     gene            12552..13823
                     /locus_tag="K8M77_000997"
     CDS             12552..13823
                     /locus_tag="K8M77_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBI7085508.1"
                     /translation="MNKLTMFLYSNIINKKIYDEFNEVLGVLKDVYVTTEDGYPRIIG
                     YKVKRDGVIFDYEFKNIDFYQKDNGKFKIQIRGSREILPRNYSYLLSQNLLDRKIVDI
                     NGKKVVKVDDLRIVKIAGEFRVIAVETGKIVKFRRNGLEGLGKLLAKVFKEKFQEKVI
                     MWEDVESLEMINDNLKLAIPYKKLQKLHPADLADILEELDAINRKKVFDSLDEDLAAD
                     TLEEIEPEVQSSIIKGLSHSKTAELFENIPNDEIADIIDELDEEEREKVLIALENEDA
                     EEVKELMGYSDESVGSIMNKDFISFNLDLTVKETIELLRELDADEEVMYYVYITDEED
                     RLNGVVTLRDLIMNEENKRLKEIMHTSSISVNIDSTIEDAIEKSTKYDLISIPVVDYE
                     EKLVGMVLIHDIVDELIPNNLKRKFKKKYTD"
     misc_binding    13882..14118
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14162..15064
                     /gene="cysK"
                     /locus_tag="K8M77_000998"
     CDS             14162..15064
                     /gene="cysK"
                     /locus_tag="K8M77_000998"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBI7085509.1"
                     /translation="MVFNSLLSLIGNTPILKANNLFEGNNVFVKLEKFNPGGSIKDRA
                     ALGMIEEAEKNGLLKENGVIVEPTSGNTGIAIAMIGKIKGYKVIITMPETMSKERRDM
                     IKAYGAEIILTEGSKGMKGAIEEAERLASTNGYFMPQQFLNEANAKKHYETTAEEIIR
                     DIPDLDVFIAGVGTGGTITGVGRKLKEYNPNIKIVAVEPSDSAVLSGENSGPHKIQGI
                     GAGFVPKIYDSSVVDEIIKVKNEDAYTMAKNFADKEGILVGISSGAALKGAKYILKNI
                     EKDKKVLVIAPDGGEKYFSTGLYE"
     gene            15083..15592
                     /gene="cysE"
                     /locus_tag="K8M77_000999"
     CDS             15083..15592
                     /gene="cysE"
                     /locus_tag="K8M77_000999"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBI7085510.1"
                     /translation="MFKNLKYDIENVMKNDPAARTKLEVLLLYQSIHVLIFYRIAHGL
                     YKIKLFFLARLISQLGRFFTGIEIHPGAKIGKGIFIDHGMGVVIGETAEIGDNVTIYH
                     GVTLGGTGKDKGKRHPTIGNDVIIGCGAKILGPISIGDGAKIGANSVVLKNVPKGKTA
                     VGIPAVIKN"
     gene            15737..16729
                     /gene="queG"
                     /locus_tag="K8M77_001000"
     CDS             15737..16729
                     /gene="queG"
                     /locus_tag="K8M77_001000"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBI7085511.1"
                     /translation="MKEKILEFCKSLGLDTVGFVKCRRFSELEEVYNIRKDKGFENEF
                     EEKDIEKRVNPNVYMEDGKTIITIAFPYLYDEDYVDNGFSVYTRGMDYHYVVSNFLKK
                     ISEYIESLGGRAISLVDSNSLPERYIAYLGNIGFIGKNNMLITKKYGSYVFLGEIITD
                     LEIQCEEERTLEELRTHKECGTCEICYKNCPTKVLNRSKMKNTNSCMSYITQKKDIED
                     KYLKLMKGRIFGCDSCQKECPYNKEITYTNIEDFKPLTFMQKDSFEDFHKISKKEFNE
                     TFKKTSCGWRGKNTILRNIMIKKVLIDKEDISNYELNSESLKNFRNRLLNFYDV"
     gene            16767..17471
                     /locus_tag="K8M77_001001"
     CDS             16767..17471
                     /locus_tag="K8M77_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBI7085512.1"
                     /translation="MFTRIGANILVRLPDSIFKPIAGKLIGGCLDKYADLNVYGLDKI
                     KKEEGPFIFIGNHLSNADGLVLDKVLQKEYDPYFIAGVKLNDEALTNIGTRLVKNIKI
                     KPNSADRESLSKIVKAVKSGENIVIFPEGTRSRNAKMIEAKKGIILIARLTKAKIVPF
                     GMTGTEKLMPINDENMGSEKFHHSKVNIRFGTPIDLPKREKDEGKHEYEERALNYLMK
                     NIAKLLPEDYRGVYKD"
     gene            17483..18112
                     /gene="nth"
                     /locus_tag="K8M77_001002"
     CDS             17483..18112
                     /gene="nth"
                     /locus_tag="K8M77_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBI7085513.1"
                     /translation="MKKRTKEVLEILKEEYPDAKCELNYETPFQLLVATILSAQTTDK
                     KVNEVTKGLFKDYPDVDSFLTISQEELEDRIKQIGLYRNKAKNLIMMVRQLKENFGGE
                     VPKTMEGITSLAGAGRKTANVVLSNAFGVPSIAVDTHVFRVSNRIGLAHSDNVLETEK
                     QLQKELPKKEWSLAHHLLIFHGRRCCMARKPKCDICKINKYCDYFKNNR"
     gene            complement(18141..19004)
                     /locus_tag="K8M77_001003"
     CDS             complement(18141..19004)
                     /locus_tag="K8M77_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI7085514.1"
                     /translation="MNNKTKGIILITISALSFALMSTFVKLSGDLPSIEKSFFRNLVS
                     CFVAFYLVKKDNAVLFGQKENRLALIGRSALGTLGIWANYYAIDRLILSDATILNKLS
                     PFFVIIFSYLFLKEKLKPIHIICLLAAFSGVLFIVRPSGNLASNFIPAIIGLCSAAFA
                     GGAYTFVRYLGGKEKGATIVFFFSFFSIVTTFPIMLFQFETFSLEQFVFLILAGVAAS
                     CAQFALTAAYKYAPARDISIYDYTQVIFTALIGFVLFGDIPDKISLIGYALILLASFS
                     IFLYNKKEANK"
BASE COUNT         7584 a         2020 c         3351 g         6098 t
ORIGIN      
        1 accttaattt tacataggca taaatttatt ttacatatgt ttagtttaga tgtggtctta
       61 attttattaa gttagataat tcgtaatttt aggagaagga tatgagaaga gagtttgagg
      121 tattaaataa tatttttgga tatagggaat ttagaaaagg gcaacaggag gtaatagata
      181 agatcttaag tggaaaggat gtattttgta tattacctac tggaggagga aaatccttat
      241 gttatgaaat acctgcttat atttttaaag gaacaacact tgttatttct cccttaatat
      301 ctcttatgaa ggatcaagtt gataacttaa attccttagg aattaatgcg gcttatataa
      361 gtggaggaaa tgactttgaa gaggttaaaa atataattag gaaattcata aaaggagagt
      421 ttaggcttct ttatgtatca cctgagagat tagaaaatag atatttttta gataatatag
      481 aagaagctaa tattgcacaa attgtagttg atgaagctca ctgcgtatca atgtggggac
      541 atgactttag aaaaagttat agcaagataa aaccatttat ttgtaatcta aaaaaaagac
      601 caattattac agcctttaca gctacggcta cagagacagt tatgaaggat tccattgaac
      661 ttttaggtct tcataatccc tttatttata aggggagctt tagtagagat aatttggaga
      721 taaatgtatt aaaagaagtt gataaattag aaattataag tgaaattata agtgaacatg
      781 aggaagagtc aggaataatt tattgctcaa ctaaaaatga agttgaggaa ctttataaac
      841 atatgcttta tagagggaaa agtgttggga aatatcatgg atcattaaaa gataaagaaa
      901 aaaattatta ccaagaagaa tttttaaatg acaattttaa ggttatgatt gctacaaatg
      961 cttttggtat gggaattgat aagcctgatg ttaagtttat aatacattca acatttccaa
     1021 aaagtataga aaattattat caagaaatag gccgtggagg aagagatgga agcttagcta
     1081 agtgttatct tctttattca gagcaagata taagagtcat ggattattta ataagttcaa
     1141 ctacagaaat atctagaaga actatagagc ttaaaaaatt agaaaaaata atagagtttt
     1201 gtaattatga taagtgtctt agaaaatata ttttagatta ttttggagag gaaaattcca
     1261 taaagtattg caacaattgt actaactgct taaaaaatag tgatttaata gatatgacat
     1321 tagaggcaca aaaaatatta tcatgtatat atagaacaaa ggaagccttt ggagaaagtg
     1381 ttttaataga tattttaagg ggaattcatg ggccaaaggt tgaaaagtat aagctatatg
     1441 agctttcaac ctttggaata atgaaagaat atacaagtaa gtatataaag gacataataa
     1501 aggagctttt atctattaag gctttagaga gaaaagaagg aacatattct atggtaaaat
     1561 taaatagaaa atctatagga atattaaagg gaaaagaaaa ggtattttta gatgttaata
     1621 ataatgaaga aagcatgtgc atggatttag aacttttcaa gaaacttcgc attttaagaa
     1681 aagatatatc aagaagagaa ggagtaaaac catacatagt ttttacagat tcaatgatta
     1741 tggagataat aaataaaaat cctaaaagta aagaggattt aaaaaatatt aggggatttg
     1801 gagagcagaa aataacaaag tatggccctt ttattcttac aaccttaagg gactatgaaa
     1861 agtatggaaa aagaaattag ttttactaat ttcttttttt gttttataag gatattttgt
     1921 catagaacat aatagataag gaatatattt aggttaaagg agggattaca atggatttta
     1981 aagagtttat aaaaagtgat agggaaaaac ataataagca gaagtttaag ggaacatttc
     2041 ttgattattt agaaattgta aaaaataatc ctcatgttgc aaaactttct cataaaagga
     2101 tttatgatct tattatggat aagggctttg aaatattaag acctgaggaa aatgcaaaaa
     2161 ttaaaaagat atatggtaat gaaaaaataa agaaatataa cttttttaaa gaagattttt
     2221 atggaattga tacagttatt atgaaattaa tgaattattt tcattcagct tcaatgaggg
     2281 gagaagaagc aagacaagta ttatacttag taggacctgt gggagctggt aaatcctctt
     2341 tagtagaatc aattaaaaga gttttagaaa cagcaccgcc agtatatgtt ttagatggat
     2401 gtcctatgca tgaagagcca ctacatttaa tacctaaaca tttaagaaaa gaatttgagg
     2461 atatgttagg tgtcgagatt gaaggagatt tatgtccagt ttgtaagtat agattattaa
     2521 atgaatttgg tggagaatat gaaaagtttc cagtttcaac aaagaatttc tcaattcgtt
     2581 ctaggaaagg aattggagtt gtacctccag tagatcctaa taatcaagat acctcaattt
     2641 taacaggatc tattgatatt tctaaaatgg acatgtatcc agaggatgat ccaaggatat
     2701 tctcattaaa cggtgcattt aatgttggta atagagggtt agttgaattt attgaagtat
     2761 ttaaaaatga tgtggaatat ttacacacca taataactgc aactcaagaa aaatctattc
     2821 cttcaccagg taagggatca atgatttatt ttgatgggct tataatagct cactctaatg
     2881 aagctgagtg gaataaattt aaatcagatc atactaatga agcaatctta gatagaatag
     2941 taaaaattga agttccatat tgtttagagc ttagtgaaga aattaaaatt tatgaaaaaa
     3001 tattaaagaa atctaatttt gatgctcata taactcctca taccatggaa attgcttcaa
     3061 tgtttgctat attaacaaga ttgcttcctt ctatgaaagt tgatccaatt actaaactta
     3121 aactttataa tggagaggaa attgttgaaa agggaacaac aaagagaatt gatgttttag
     3181 agttaaaaga agaagctgga actatggaag gcatgaaggg aatttcaaca agatttatta
     3241 taaaggcaat agataatgct ctatcaaatg cagagcataa gtgtataaat ccattaagtg
     3301 ttatggaaag tataataaaa tctgttaagg atatggatat atctcaagag gataagaaga
     3361 aatatttagg atatattcaa gatacaataa gaaaagaata taataagatt cttgaaaaag
     3421 aaattacaaa ggcatttatt cactctttta aggagcaagc agaaagttta tttaataatt
     3481 atatagataa tgcagaggct tatgtaaata aaacaaaact taaggattct tcaactagtg
     3541 aagagttaga gccagatgaa gaatttatga gaagcataga ggaacaaata ggaatatcag
     3601 aatcttcttc caaggggttt agggcagatg taacatcata catgttctat atagttagaa
     3661 gtggcggaaa aatggactat agatcttatg aaccattaaa agaagccatt gaaaagaaac
     3721 taacagcttc agttaaagat ttatcaagaa taataactaa atctagagta agagataaag
     3781 atcaagatag aaagtataat tcaatggtaa atcaaatgga gaaaaatggt tattgtccac
     3841 attgctgcga tgtaatatta aaatatgcag ccaataacct atggaaggat taaaatatgg
     3901 ccatatttag agatcaagca gaaaaccatg tagaacatga tagatctata gaggatagaa
     3961 gacgtcacag gcagttagta gaaaaatcta ttaaagaaaa tttaggggat atactttcag
     4021 aggagagcat aattggagag actaaaaata aaaaatataa gattcctatt agaggaataa
     4081 aggaatatca atttatttat ggtgcaaata ataaaggggt aacaacaggt actggagaag
     4141 aaagtcgagg ggatagaatt tctagtgata aaagaaaagc tatttctaat aataaagcag
     4201 gaaatcagga gggaaaggat atatatgaaa ctgagataac cttagaggaa cttatggatt
     4261 atatagttga ggatcttgat ttacctaact tagataagaa gaagtactca gaaataatag
     4321 ttgaaagtgc agctaaaaaa agaggatatc aaaaatatgg tgtaagacca agacttgcaa
     4381 agaaaaaaac tgttatgtgt aaaatagcta gaaaacaagg aaaaaaaaga gcattgcgtg
     4441 aaataggaga agaagcgaaa ataggaagat ttccttttag agaagatgac ttaagatatt
     4501 ataaagtgaa aaaacatcct aaaaaagaaa gcaatgctgt aatgattttt ataatggacg
     4561 tttcaggttc tatggataat actaaaaaat atttagctag atcatttttc tttgttttat
     4621 ctaggtttat aagaagaaaa tataataatg tagcctttga atttatatct catactacta
     4681 cagctaaaaa tgttaatgaa tatgagtttt tccacaaagg ggaatcagga ggaacttata
     4741 tatcttcagg aataaatgct gccatagatt taataaaaga aaagtataac ccaggggttt
     4801 ggaatatata tcctttctat gcttcagacg gtgataactg gagtgaggat aatgaaaagg
     4861 ctatggaagc tgtaaatgaa atttcagatt taagtaatat gtttggatat atagagcttt
     4921 taccatcaac ttattctact acaatgttct acagatttaa aaaggaaata agtaaggaaa
     4981 attttgtctc tgtaactgta aaggaaaaga aggatctgtg gaatgctata aaatatatgc
     5041 tatctgaaga actacaggaa aagaataagg aatgagagtt ttaagataag gggctttaaa
     5101 tatggattat aatttaaaag atttagaagg atggaacaat aagatagaag aaatagcaaa
     5161 gagtgaaggg ttagattatt atcctcagga atatgaaatt ataggttata atgaaatgct
     5221 aagttatgaa gcatatgtag gaatgccttc taaatatcca cattggagtt atggtaaggc
     5281 ttatgaaaag aataaaactt tatattcatt aaatatgaca ggattaccat atgagatggt
     5341 tataaattct aatccttgcc tagcttattt aatgaaggat aatactttat tgcttcaaat
     5401 attaactatg gctcatgtat atggtcataa tgattttttt aaaaataata gattgtttaa
     5461 aaaaggaaca gatgcaaata atgctttaga aatgtttaag ctagatgcag atactataag
     5521 aggatatata aatgatccaa gtataggtta tagccaagtt gaaagaatat tagattctgc
     5581 tcattccata agatatcaag tgccaagggt tataggtgaa aagagggagt cgcaagatga
     5641 gcagagaaaa agagtattag agatattcaa taagaaaaaa gaaaataggg gtattttaga
     5701 ctctatggaa gaaatacttc ctcctgatat aagtaaaatt ccacttaatc cagatgatga
     5761 tattattcag tttataatag attatagtaa tttagaagag tgggaaaaaa atattctaaa
     5821 aatagttaga agagagagct tatacttttt accacaaata gagactaaga taatgaatga
     5881 aggctgggca agttattggc attataatat tttgaaaaag ttaaatttac cacagggact
     5941 tcatttagag tttttaaaaa gacataatga tgttgtggct cctgtttctt atggacttaa
     6001 cccttattat ataggtttta gaatttttca ggatatagaa aaaaggtatg gcaaagaaaa
     6061 aatctttgaa gtaagagcat tagaaagaga taattccttt ataagaaaat atttaactag
     6121 ggagctttgt gaggagctta atttattcca gtatgttaaa aagggatttg attatgttgt
     6181 agaggaagtt tctgatgatt taggatggaa gagtattagg aatcttattg cagatgaatg
     6241 cggtgtaagc tctattccac atattagagt tagggagcta aataaaaaag atatgacttt
     6301 aaatttagag catgtttttg atggaagaga attagaagct aattatgcaa agaaaacctt
     6361 aaagtatata aatattttat ggggaagaaa ggtaaattta agcagtaagg ggaaagatgg
     6421 atcagatata ttatttatgt gtgatgaata ggagaaaact ttcatattat agaatataaa
     6481 taatataaaa tatggagtta atatgaaaag tttaaaagga tctcagactg aaaaaaatct
     6541 ttacaaaacc tttgcaggag aatgtcgtgc tcgaacaaaa tataatttgt ttgctgaaga
     6601 ggcaagagag gaagaacaac attggatagc agatatcttt gatgaaactg ctaaaaatga
     6661 atatgcccat gctagagaag tttttagaag atttttaggt aatgtaggag acatactaga
     6721 taaccttatg gtagcagcta tgggggaaag tgatgaattt aaagaaatat ataaagagtt
     6781 tgaagaaaca gcacgtagtg aaggctatga ggaaatagca gactttttta aggaacttag
     6841 agaggtagag gaatctcata atgaaagata tatggacttt tatgataagg ttaaatcaaa
     6901 aagtttatat tcatcaaaag aacctgtgaa atgggtatgt acaaattgtg gatatattca
     6961 tgaaggaaaa gaagctccaa tgaattgtcc tttatgtaga ttccctcaag gatattttag
     7021 acttttaaat gaatgttagt attaaagagg agacttttaa atgggacaaa tcgagatttt
     7081 tagaaaacat tttaaagagg ttaataattt aagtgttgtt ttaagtagct atgctaatac
     7141 ttatagactt ttagttggag ctgctggaga attaaataat atatctaaag ttagaaagag
     7201 agatttagat gatgccttag atagagttga tgaaatggga aagataatag attctatttt
     7261 agaggttatt aatgacaatg aagaggctta tattaagtat ataaaactta aatctaaatt
     7321 tataatggaa aaggcttcaa aggatataat ccaaactgaa gtagagcaag acttactttt
     7381 tgaaaactct aatagagagg aagaagaata aataataaaa atcctattaa agaattttct
     7441 ttaataggat tttttgttaa aatttcttta attgtaaatt actaaccatt aagtaagaga
     7501 atataactaa aattactact gggaataaaa tatttaaatt aaactttatt gataataaaa
     7561 taaataaagc aacaacagct ccagcaatag ttataggtac tcctctaaat acaccgtcaa
     7621 attcagttgc attgtatcta gctaatctaa aagctccaca aatagggaag caaagtaata
     7681 atgcataacc tataattccc ataggaccta aagattcaaa attataaaat acaaaagtta
     7741 gtactgatgg tgccacacca aatgatacta aatctgctaa agaatctagt tccttaccaa
     7801 gatcactaga aacatttaaa taacgagcaa ctcttccatc atatctatca actaatccag
     7861 caagaagtat gaatattgct gctaataaat actcagattt aaatgttgct aataatgata
     7921 atataccaaa agataggttt ataaaagtaa atatatttgg tatactactt tttctcatat
     7981 aatcactcct aataaaaata ttaacaataa taaaattata ttaatgtaaa tcccaaaatt
     8041 aaatacatat taaaataact ttgaattaat tgtgaataaa aattgaaaca tacatattat
     8101 tacacattat tacacaatta ttaatataag tcaaaatcta attacttata tcacattatc
     8161 gacaatagga ttaacaaatg atataatttt ataatattgt agggtttgta taatatatta
     8221 aaaatgatac cattaattaa aagaaatatc aatttaattt atttatagga agtaattaaa
     8281 aggtggaggg taagacattg aagaaagtaa gatttatata taatccatat tcgggcgaga
     8341 atgctatttt aaaggaactt gataaaataa ttactatgca tcaagataag ggatatacta
     8401 tagttcccta tagaattcaa aggggagtag atgtaataaa tgctctagac ataatagact
     8461 ctacttatga atacatactt atagcaggtg gagatggaac tatagacact gttgtaaatg
     8521 ctatgaaaaa aagaggaata gaaattccaa taggaattat tccagtagga acagcaaatg
     8581 attttgcaaa acatttaggt atgcctcaag atgcaggtgc atgtgttgag caaatattag
     8641 attcttatgc agttggagta gacctaggta aggtaaatga taattacttt gtaaatgtat
     8701 tatctacagg gttatttact gatgtatctc aaaaaacaga tacaaattta aaaaatacaa
     8761 taggtaaatt agcatattat ttaaaaggtt tagaagaatt gcctaacttt agaaaactag
     8821 atgttaaaat aactgctaat ggcaaggtat ttaatgagcc tatatattta atgcttgtgt
     8881 ttaatggtca aacagcagga aactttaaat tagcaactaa ggccaaggtt caggatgggc
     8941 ttttagatat agttatattt aaggcttgtc atattccaga attaatacct atgtttataa
     9001 aggtattaaa gggagaacac ttagaagatt ctagaattga gtatataaga acagatgaac
     9061 ttcttataga atctgatgag gaaatagtta cagatataga tggagagaga gggccagatt
     9121 tcccattaag agttcaatgt attaaagatg gaatacaagt tttaggaata aataagtaat
     9181 ataaataata acttatagta atgagattta atatgtaaac aagataggtg agaaataaat
     9241 taggaggaat tatgaatcaa acatttattt attacacagt attgtttctt ataattttca
     9301 ttactataag taatttagta gaaaattata ggtattcacc taaaaaaata aaaaatataa
     9361 taggtatagc ttttttattg caaattataa gaatcgtatc cttaatagca ctagctttag
     9421 taagtgatca aaggtatata agtttattta aatatgtagg ctttttagat ataatatata
     9481 ttcctttaat ggcattaata gccttttata ttttacttag aagtgacaag ataaattttc
     9541 agttttttaa atttgcacca atagtgttaa cagtatttta tattggagta ataactattt
     9601 ttaaaccttt ttttaagatg tcttgggaat atggttacgt aattacaacg gaaaaacaaa
     9661 ttttacttaa atccatatat attcttttgt tagtatctat attaagcttt ttaatacttt
     9721 ttaaaaagga aaaccattct gataaaaaag gtatagtatt tttaggggtg accttagctt
     9781 ttataataat agaaaactcc ttagaattat taggagttaa atttttaaat ggtgctataa
     9841 ttagtgagct aatattatgt attttaggtt tttatatagt tttaactttt aagaaaaata
     9901 gagtttctat aaggaactgt ataaagagtt aataaaaaaa cttataaata gattatgaga
     9961 attattagat tttaagtaaa agtctagtaa ttctctttat actatttata agttttgctt
    10021 aagtaatttt ggtacattcc attatttttt gaggtaatac taagctaaaa ataactttta
    10081 aaaggttaag tggattagat ccaaaactct ttttaagata tatcatctaa tatttttcca
    10141 gaatctaatt ttataattct tgaagacact tcttttgcaa attccatatc atgagttatt
    10201 attaataccc caatattttc cttagctaag gattttataa tatttgaaac atccttagtt
    10261 aaagatgggt ctaaggctga ggtaggttca tcaaaacata tatatttagg gtttaacatt
    10321 aaagctctag aaatagcaac tctttgtttt tgcccacctg atagttcaca agggtaagat
    10381 ttagctttat cctctaaaga aagtttagaa agaatttgta atgctttttt agtagcctct
    10441 tctagagaga ggtttagagt gtgcattgga gctaaaatta agttttctaa aatattcata
    10501 tgaggaaaaa gattgaaggt ttgaaatact agtcctattt ttttatgaat ttccttttgt
    10561 gctttgaaat ctttatatat tcccttctcg caaattttta aagaatctaa ggttatatct
    10621 ccaaagtcac aggtttcaag tccatttaaa attctaagta ttgttgtttt accagctcca
    10681 gaggggccta gtaaggttaa tatttctcct tctttaagag taaaggagat gttttctaat
    10741 attttattac ccttaaagga tttctctaag ttttttattt ttatcatttt taatcctcct
    10801 aattataata tgaatatttt ttctctaacc tattaaatat tttagttaga attcctatta
    10861 acaatagata tattatgcct attaaaaaca agggaaggag agaagcatct ctgttacttg
    10921 caattttacc agctcttaaa agttcaccta tgccaactac ataaactaag gatgtatctt
    10981 taactaaagt aataacttca ttagtaattg aaggcaaagt ctttttaaat gattgaggaa
    11041 gaattatctt tttaaaggta aaccattttg aaagacctag gattttactt gcttcaaatt
    11101 gtcctttatc aatatctttt ataccagacc taaatatttc gccaaaataa gctgcgtaat
    11161 ttaaggtgaa tgctattatg gcagctggga atctatctat gctaattcct attattggta
    11221 gtccaaaaaa tataaatatt agttgtaata ataaaggggt tccacgcata actaaaacat
    11281 ataaagatga tataaagttt actattttaa tatttgataa tctaagtatt gcaactatca
    11341 ttcctaaagg aatagataaa agtagtgtta atataaaaac acctaatgta ttttttaagc
    11401 cttctattat tgatggcatt aaagtaataa tataattcaa agttaatacc tccttatttc
    11461 tctaaatctt ttccaaacca tttattagat attttagcta tagttccatc ttctttcata
    11521 tcctctaaag ttttatttaa agggtctatt aaattagaat tttttctaag tcctatgcta
    11581 taactttctt cccctaagtc ttcagtaaga acatgaaatt tactagggtc atttttaaaa
    11641 atataatact gagcaagaat ttcatcacaa acaactgcgt cacttcttcc agcttctaaa
    11701 tccataaagc aatcattaaa gctatcaaaa agaacaggct ctccattttt aaaggattta
    11761 gttagttctg gattttcaaa taaggtatca agagagctag aaccatcttg agtagttaca
    11821 gtttttcctt ttaaatcctt taaagtattt atattagaat tagaaagggt aactataact
    11881 tgtctatttt ttaagtatga atttgtaaat gcaacctttt tttctctttc aggtgtcatg
    11941 gtatatccat tccaaattaa atctatattt ccattagtaa gttcagtttc tttcattgac
    12001 caatctattg gttggaaaat aacttttaaa ttcatacgct taaaagcttc ttttgctaaa
    12061 tcaacatcaa aaccaactat gtccccattt tcatttttaa aacccatagg aacgaaagtt
    12121 tcatccatcc ctataatcac tttattacta tcctttttag tttctatttt ctttgaggaa
    12181 caaccaaaaa taagaataga taaaattact atgccaatta ataaaaataa taactttttc
    12241 atatctttct cctttcgatt tactacatta atactttatc atgatgaagc attaaagtca
    12301 aatgagaaat gtaataattt tatttaaata ttgacaaaac gttgcatttt agagtttata
    12361 taatataata aattagagag ttatgtaatt ttttatttat aggtggtgaa actaaatgga
    12421 agatagccct cgagaagata ggtgcaattt aaaattaaat attataagaa aagagggaag
    12481 ttattttcta gattaaactt ataatatact tctccttttc ttaaaataaa attaaagagg
    12541 aggagagctg gatgaacaaa ttaacaatgt ttttatacag taacataatc aataagaaga
    12601 tatatgatga attcaatgaa gtcctaggag ttttaaagga tgtttatgta actacagagg
    12661 atggttatcc aagaattata ggatataaag taaagagaga tggagtcata tttgattatg
    12721 agtttaaaaa tatagatttt taccaaaaag ataatgggaa atttaaaata caaataaggg
    12781 gtagtagaga aatattacct agaaattata gctatctttt atctcaaaat ttattagata
    12841 gaaaaatagt agatataaat ggaaaaaaag tagttaaggt tgatgattta agaatagtaa
    12901 aaatagcagg tgagtttagg gtaatagctg ttgaaactgg aaagatagta aagtttagac
    12961 gtaatggatt agagggatta ggaaaattat tagctaaagt ttttaaagaa aagtttcaag
    13021 aaaaagttat tatgtgggaa gatgttgagt ctttagaaat gattaatgat aacttaaagt
    13081 tagctattcc atataaaaag cttcaaaaat tacatccagc tgacttagca gatatacttg
    13141 aagaacttga tgccataaac agaaaaaaag tcttcgatag cctagatgaa gatttagctg
    13201 cagatacctt agaagaaata gaaccagaag tacaaagcag tataataaag ggactttccc
    13261 attctaaaac tgcagagctt tttgaaaata ttccaaatga tgaaatagca gacattatag
    13321 atgaactaga tgaggaagaa agagaaaaag tattaattgc cttagaaaat gaagatgcag
    13381 aagaagttaa agagcttatg ggatattcag atgaatctgt aggtagtata atgaacaagg
    13441 attttatatc ttttaattta gatcttactg taaaagagac tatagagctt ttaagagaat
    13501 tagatgcaga tgaagaagtt atgtactatg tttatataac tgatgaagaa gatagattaa
    13561 atggagttgt aactttaaga gatttaataa tgaatgaaga aaataaaaga cttaaggaaa
    13621 ttatgcacac atcttccata agtgttaata tagatagtac catagaggat gctatagaga
    13681 aatcaacaaa gtatgattta atatctattc ctgttgtgga ctatgaagaa aaattagttg
    13741 gtatggttct tattcacgat atagttgatg aattaatccc taataactta aaaagaaaat
    13801 ttaagaaaaa gtacacagat tagttgacaa attttaataa aagaatataa taaagtatat
    13861 aattcatatc aaaatagtcg gataaatgtt gtgataaagt aaagtattat aaggataggt
    13921 cacagagaga aagtggtgct ggaaatcttt caccaaagcc ttataagaag tgcgcttttg
    13981 aacaggaatt tcgaaaagtt ataagtagaa attttcgagt atcacccgtt aacgtgatag
    14041 agtatttata gtacttgatt aagtgaggtc tttgcctaat tagagtggaa ccacggagta
    14101 aaaaacttcg tctctaaata gtataagacc tttttattat atttttaagg aggaaattta
    14161 tatggttttt aacagtcttt taagtttaat aggaaacaca ccaatattaa aagcaaataa
    14221 tttatttgaa gggaataatg tatttgttaa gcttgaaaaa tttaatccag gaggaagtat
    14281 aaaagataga gctgctttag gaatgataga agaagctgaa aaaaatggat tattaaaaga
    14341 aaatggagtt atagtagaac caactagtgg aaacacagga attgctatag ctatgattgg
    14401 taaaattaaa ggatataaag ttattataac tatgccagag acaatgagta aagaaagaag
    14461 agatatgatc aaagcttatg gtgcagagat aattttaact gaaggaagca aaggaatgaa
    14521 gggagctatt gaagaagcag agaggttagc tagtacaaat ggatatttca tgcctcaaca
    14581 gtttttaaac gaagctaatg ctaaaaaaca ttatgaaact actgcagagg aaataataag
    14641 agatattcca gacttagatg tatttattgc aggggtagga actggaggaa ctattacagg
    14701 agtaggaaga aaattaaagg aatataatcc taatataaaa atagttgctg tagaacctag
    14761 tgattcagct gtattatcag gagaaaattc tggtcctcat aaaattcaag gaataggtgc
    14821 tggattcgta ccaaaaatat atgattcatc agtagttgat gaaataataa aggttaaaaa
    14881 tgaagatgca tacactatgg caaaaaactt tgcagataaa gaaggaattc tagtaggtat
    14941 atcttctgga gctgcattaa aaggggcaaa gtatatttta aaaaatatag aaaaagataa
    15001 aaaagtatta gttatagcac ctgatggagg agaaaaatat ttttcaacag ggctatatga
    15061 gtaatttgta ggagtgaaaa ttttgttcaa aaatttaaaa tatgatatag aaaatgttat
    15121 gaaaaatgat ccagcagcta gaacaaaact agaggtttta ttactttacc aaagcataca
    15181 tgttttgata ttttatagaa tagcacatgg actatataag ataaaattat tttttctagc
    15241 acgattaatt tctcagttag gaagattttt tacaggtata gaaattcatc caggggcaaa
    15301 aataggaaaa ggaattttta tagatcatgg aatgggtgtt gtaataggag aaactgcaga
    15361 aataggagat aatgtaacta tatatcatgg agttactctt ggaggaactg gaaaggataa
    15421 aggtaagaga catccaacta taggaaatga tgttattatt ggatgtgggg ctaaaatttt
    15481 agggcctata agtataggcg atggagcaaa aataggagca aactctgtag tattaaagaa
    15541 tgttccaaag ggaaaaaccg ctgttggaat tccagcagtc attaaaaatt aatttataaa
    15601 tatgccaatt tttattattt ggtttataat aagactatat ataaatttta ttagttagag
    15661 aaaaattaaa accatatgtt ttacatttac taactaaaaa ttttaatata gtctttttaa
    15721 tttgaggtga taatttttga aggaaaagat tttagagttt tgtaaatctt taggattaga
    15781 tactgtagga tttgtaaagt gtagaagatt ttcagaactt gaagaggtgt acaatataag
    15841 aaaagataaa gggtttgaaa acgagtttga ggaaaaggat atagagaaaa gagtaaatcc
    15901 taatgtgtac atggaagacg ggaagactat aataacaatt gcttttcctt atttatatga
    15961 tgaagactat gttgataatg gattttcagt ttatactaga ggaatggatt atcattatgt
    16021 tgtctctaat ttccttaaga agataagcga atatatagag tctttaggag gacgagcaat
    16081 ttctttagtt gatagtaact cattacctga aagatatatt gcatacttag gaaatatagg
    16141 attcataggt aaaaacaata tgctaatcac taaaaagtat ggttcctatg tgtttttagg
    16201 agaaataatt acggatttag aaattcagtg tgaagaagaa agaactttag aggaattaag
    16261 aactcataag gaatgtggta cctgtgagat atgctataaa aattgtccaa ctaaggtttt
    16321 aaatagaagt aagatgaaaa atactaattc atgtatgtct tatataactc agaaaaaaga
    16381 tatagaagat aaatatttaa aacttatgaa gggaagaata tttggttgtg atagctgtca
    16441 aaaagagtgt ccatataata aggaaataac ttatacaaac atagaagatt ttaaacctct
    16501 aacttttatg caaaaggatt cttttgaaga ttttcataaa ataagtaaaa aagaatttaa
    16561 tgaaactttt aaaaagactt cttgtgggtg gagaggtaaa aatacaattt taaggaatat
    16621 aatgattaaa aaggtcttaa ttgacaagga agatatatca aactatgaat taaattctga
    16681 aagtcttaaa aattttagaa atagactttt aaatttttat gatgtataat aatatattgt
    16741 taaaatttga ttcaggaggt aaagttgtgt ttactcgtat aggggctaat atattagtta
    16801 gattaccgga tagtattttt aaaccaatag caggaaagtt aataggtgga tgtttagata
    16861 aatatgctga tcttaatgtt tatggacttg ataaaataaa gaaggaagag gggccattta
    16921 tatttatagg gaatcattta agcaatgcag atggattagt tttagataag gttttacaaa
    16981 aagaatatga tccatatttt attgctggag taaagctaaa tgatgaggcc ttaactaata
    17041 taggtacaag attagttaaa aatataaaaa taaagccaaa ttcagcagac agagaatccc
    17101 taagtaaaat tgttaaggct gttaagagtg gagaaaatat agttattttc ccagaaggaa
    17161 caagaagtag aaatgcaaaa atgatagaag ctaaaaaggg aattatactt atagcaagat
    17221 taacaaaggc caaaatagta ccttttggaa tgactggaac tgaaaagctt atgccaataa
    17281 atgatgaaaa tatgggatca gaaaagtttc atcattcaaa ggttaacata aggtttggaa
    17341 ctcctattga ccttccaaaa agagaaaaag atgaaggaaa gcatgagtat gaagaaaggg
    17401 ctttaaatta ccttatgaaa aatatagcaa agcttttacc agaagattat agaggggtat
    17461 ataaggatta gggggatata ctatgaaaaa gagaactaaa gaggtattag aaatattaaa
    17521 agaagagtat ccagatgcta aatgtgagct aaattatgaa actccctttc aacttttagt
    17581 tgctactatt ttatcagcac agactacgga taaaaaggtt aatgaggtaa ctaaagggct
    17641 ttttaaagat tatccagatg tagattcttt tttaactata agtcaagagg agctagagga
    17701 tagaataaag caaataggac tttatagaaa taaagctaaa aatttaatta tgatggttcg
    17761 tcaattaaaa gaaaattttg gtggagaagt tcctaaaact atggaaggaa taacttcttt
    17821 agctggggca ggaagaaaaa ctgccaatgt ggttttatca aatgcctttg gagttccatc
    17881 tattgcagta gatactcatg tatttagagt ttcaaataga attggtttag cacattcaga
    17941 taatgtactt gaaactgaaa aacaacttca aaaagaatta cctaaaaaag aatggtcctt
    18001 agctcatcat cttttaattt ttcatgggag aagatgttgt atggctagaa agcctaaatg
    18061 tgatatatgt aagatcaata aatattgtga ttattttaaa aataatagat aaaaagctac
    18121 acaaaatagt gtagcttttt ttacttatta gcttcctttt tgttatataa aaatatagaa
    18181 aaagatgcta ataaaattaa ggcatatcct attagggata ttttatctgg tatatctcca
    18241 aataatacaa atcctattaa ggctgtaaaa ataacttgag tatagtcata aattgaaata
    18301 tctcttgctg gagcatattt ataggctgct gttaatgcaa attgagcgca agaagcagca
    18361 acccctgcaa gaattaaaaa tacaaattgc tctaagctaa aggtttcaaa ttgaaataac
    18421 ataataggaa aagtagttac tattgagaag aaagaaaaga agaaaacaat agtagctccc
    18481 ttttctttac cacctaaata tctaacaaag gtataagcac ctccagcaaa agctgctgaa
    18541 caaagtccaa ttatagcagg aataaagtta cttgctaaat ttccagaagg tctaactata
    18601 aataaaactc cagagaatgc agctaaaaga catattatat gaattggttt taatttttct
    18661 ttcaggaata aataagaaaa tataataacg aaaaatggac ttaatttatt aagaatagta
    18721 gcatctgata atattaatct atcgattgca tagtaattag cccaaattcc taaggttcct
    18781 aaggcagaac gtcctataag agcaagtcta ttttccttct gtccaaaaag tacggcatta
    18841 tcttttttca ctaaataaaa tgccacaaaa caactaacta agtttctaaa gaagcttttt
    18901 tctattgacg gtaaatcacc ggataatttt acaaatgtac tcataagagc aaaactaagt
    18961 gctgatatgg ttattaaaat tatacctttt gttttattat tcatttaatc ctcctattaa
    19021 attgttgaaa tttttataat aacattttag atg
//
LOCUS       DAEVGI010000031        19051 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEVGI010000031 DAEVGI010000000
VERSION     DAEVGI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 19051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19051
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.031"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            229..591
                     /locus_tag="K8M77_001004"
     CDS             229..591
                     /locus_tag="K8M77_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085515.1"
                     /translation="MEKDIKINEGLISICNDDYLNAMSLVKEKLNIYLEEPKVFSNEY
                     ILGTPGFKDNLRDNLKRALAIIFDFVAVYKKYEVNENIKNILEKARNIYAILEEAQGN
                     INEDVLKSLKEIRAIEFK"
     gene            788..1774
                     /locus_tag="K8M77_001005"
     CDS             788..1774
                     /locus_tag="K8M77_001005"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBI7085516.1"
                     /translation="MSVKESVLKILEENRERSISGEELAKHLSVSRAAIWKTIKSLRG
                     EGYNISAVTNRGYQLTKDNDLISAEGIKIFLNPKYRENYIRVYKTIDSTNQEAKKLLM
                     DNDIPHGTILIAEEQTAGRGRFQRKFFSPFNKGIYMSVILRPNIELSKAIHITTSAAI
                     SVCRAIETLTKKRPKIKWVNDIFLDDKKICGILTEATGNFESGRVENVVVGIGVNFKN
                     DSKDFPEDIKNIAGSIFGGEKPTITRNQLIAEILNELFELCENLEDKSIMTEYRILSS
                     VLGKEVSFLKNNKLNKGLAIDITDEGALVVKYENGEIDYLNSGEISVKTLNK"
     gene            1931..3016
                     /locus_tag="K8M77_001006"
     CDS             1931..3016
                     /locus_tag="K8M77_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HBI7085517.1"
                     /translation="MLKEIIFGISSIKGPTWLKEFNENNKQLEDLKILSGKVYSEKKK
                     LIDRDIMYLTYGIEGEKNVSYEIKNSYIPMLALHDIRIEDGESVAQMDYILITKSFIL
                     VLETKALSGDITVNESGEFIRYFKNKEGKVYRKEGIYSPVAQNERPVRVLTEYLKKNK
                     MIKKYPIYSCVVIAKSKSVINKYKAPKEIKSQIIKYDQLTSFIKEKLNYHKIKQDFKV
                     LDKVEYSIGKFLLENNKPVEFDYYKKYSLSEEDFIKKESEVCSEEKSNNDNFNDEVLF
                     NRDNKSYEGKILINKNKEELRRKLKQLRINFAIKEKLPSYCIFNDETLNDLLDKMPKN
                     KEELMKVKGLAKVKINKYGEEILGVLN"
     gene            3177..3911
                     /locus_tag="K8M77_001007"
     CDS             3177..3911
                     /locus_tag="K8M77_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HBI7085518.1"
                     /translation="MPFTLAHPAAVVFFKNKKFNLTALILGSMAPDFIYFLNFRPYGN
                     LGHEILGFFILNLPLCILLAYIFHNFIKSPLISHLPRPLDNWYGYLRTEQFGLHSWKE
                     VIVFIYSALLGMITHVLWDGFTHKTGFFMRHITWLRQKIIIFGADEIYIYKLVQHTST
                     VIGIIIVLFFLYKLRDSNTRILWGHKSFKDKFIYDLIVITTSIIVLVLSYIIFKYLSI
                     PYGIGNLVVSSINGIFIGILIASFKK"
     gene            4101..4406
                     /locus_tag="K8M77_001008"
     CDS             4101..4406
                     /locus_tag="K8M77_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085519.1"
                     /translation="MDRMLLLQNILEALVFVVSTILFAVLYGLIDKKIRLTDKIYGAF
                     KIEPKWEWIIHIVIALILIVLVHYFGVYIFKIWNLATVFITGAIAGICIYNMNRNNF"
     gene            4652..5302
                     /locus_tag="K8M77_001009"
     CDS             4652..5302
                     /locus_tag="K8M77_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBI7085520.1"
                     /translation="MVGEQRVRDVYIQYDKNGETPKYETPLAAGADLFAAEDMVIRPF
                     EKVAIPLGLKFAIPDNMELQIRARSGLSLRTNLKISNSIGTIDADSVSDISVILENNY
                     NIANLPYEIANDISILDDLKNNYIEVRLSDYLESKGHDISDYEDEGKKAYKKLLKTYI
                     YLDKNGNPYGTIYIKKGNRIAQMVLAKYYKANFIKTDDVSKFKSVNRGGGFGHTGF"
     regulatory      5556..5660
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5809..7119
                     /gene="thiC"
                     /locus_tag="K8M77_001010"
     CDS             5809..7119
                     /gene="thiC"
                     /locus_tag="K8M77_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBI7085521.1"
                     /translation="MNYTTQMDAAKKGIITKEMQVVAEKEGINIETLMNLMAEGKIVI
                     PANKNHKSISAEGVGQGLKTKINVNLGISKDCANIELELEKVKKAIDMNAESIMDLSN
                     YGKTYDFRKRLVEVSTAMIGTVPMYDVVGFYDKELKDITVDEFFDVVEKHAKDGVDFV
                     TIHAGLNRETIETFRRNKRLTNIVSRGGSLLFAWMELNNRENPFYEYFDRLLDICEKY
                     DLTLSLGDACRPGSIADATDAVQIKELITLGELTKRAWERNVQVIIEGPGHMAMNEIE
                     ANVLLEKKLCHGAPFYVLGPIVTDIAPGYDHITSAIGGAMAASYGADFLCYVTPAEHL
                     RLPNLEDVREGIVATKIAAHAADIAKGISGARDIDNKMSDARKRLDWDEMFSLAIDSE
                     KAIRYRKESTPEHKDSCTMCGKMCSIRNMNKILEGKDINLLRED"
     gene            7358..11158
                     /locus_tag="K8M77_001011"
     CDS             7358..11158
                     /locus_tag="K8M77_001011"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBI7085522.1"
                     /translation="MSGIRMVFVEKKAGFNVESQILLKDFKDNLGIEALEDVRVLNKY
                     ILGDMEEEQYVRTVNTILSEAPVDRVYEENFEIGQDEIAFGVEYLPGQYDQRADSASE
                     CIMLLTEEEKISVKSSKVIILKGNLNEEEIKKIKSYYINPVDSREVSPLSKVLEENLE
                     EPNDVEVLDGFLDLNEEGLKNFHRDKSLAMSLEDLKMIRDYFKSEDRNPTITEIKVID
                     TYWSDHCRHTTFETIIKDVYIEEGKYSEPIKKAYEDYKNSRAYVYGENLNNKEVKLMD
                     LATIAMKELRKRGELDDLDVSEEINACSINIEIETDKGTEEYLLMFKNETHNHPTEIE
                     PFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPTVEICETLKGKLPQRKITLGAAH
                     GYSSYGNQIGLATGQVSEIYHPNYAAKRMEVGAVIAATPKENVIRLKPSKGDIVILLG
                     GRTGRDGIGGATGSSKEHTEESINQCGAEVQKGNAPTERKIQRLFRNKEVAQMIKRCN
                     DFGAGGVSVAIGELCRGIDIDLNKVPKKYEGLDGTELAISESQERMAVVISSENADRF
                     IKLSEEENLEATIVAEVTDTDRLRMNWKDKTIVDIKRSFLDTNGAKQEISLKVKSPSA
                     YPYEIKNCDVKEEWLKSLRNLNVCSQKGLIERFDSTIGGGTVLMPLGGKYQLTPAEGM
                     AAKIPVLGGESKDASLMTYGFNPYLGVWSPFHMAFYSVIESVTKISAMGGDYKKVRLT
                     FQEYFEKLLRDEEKWGKPFAALLGAYKAQMDLGLPAIGGKDSMSGSFGELNVPPTLVS
                     FAVGLEKASRIISPEFKNIGSTLVLMKGEKLEDGTLEIEGFKNNLEKLYELIGEEKVV
                     SAYSLKFGGVSEGITKMSLGNRIGATLNNISKEELFGFNYGSLILEAKEGVNLEEEFK
                     GTNYKVIGNTIEADVIKCEEYDFEVSLEELEKSYEEKLEYVFKSKTEDKEGGFSDLIS
                     NDKDGANILDNGQMHIEEKLKSKITRVEKPRVVIPVFPGTNCEYDCRRAFEKEGAEVS
                     EVIIRNLNKEALSDSINMLKKEIDKSQIIMLPGGFSAGDEPDGSAKFIATIFRNPKIK
                     DSVMKLLNERDGLILGICNGFQALIKLGLLPYGKIIDIEEDMATLTYNNINRHMSSIV
                     RTKITSKKSPWFNEVSLGEVHSIPISHGEGRFVAPESLIKELVENDQIATQYVDLEGN
                     MAMNMPYNPNGSSLAIEGITSRDGRILGKMGHSERIGDNLYKNIPGEFDQKLFKSGVD
                     YFRK"
     gene            11481..11960
                     /gene="purE"
                     /locus_tag="K8M77_001012"
     CDS             11481..11960
                     /gene="purE"
                     /locus_tag="K8M77_001012"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003403061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBI7085523.1"
                     /translation="MKVAIFFGSKSDIDVMKGAGNALKEFGIPYNAYILSAHRVPEKL
                     IETLEKIEKEGCEVIIAGAGLAAHLPGVIASHTILPVIGVPVRAAVEGMDALLSIVQM
                     PKSIPVATVGINNSYNAGMLAVQMLSLKYPELREKLIQFRIDMKHKFIAENGEGVEL"
     gene            11960..12667
                     /locus_tag="K8M77_001013"
     CDS             11960..12667
                     /locus_tag="K8M77_001013"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBI7085524.1"
                     /translation="MVNQLEMLYEGKAKKIYATDKEDMVIVHYKDDATAFNGEKKAQI
                     ESKGVLNNEITSLIFEMLNKEGIKTHFVEKLNDRDQLCKKVEIVPLEVIVRNVAAGSM
                     AKRLGLEEGYELKTTVFELSYKDDSLGDPLINDYHAVGIGATTFEELNKIYEITAKVN
                     EILKEAFKKQNINLIDFKLEFGRYKGEILLADEISPDTCRFWDAKTGEKMDKDRFRRD
                     MGNVINGYREVLNRLRN"
     gene            12689..14134
                     /locus_tag="K8M77_001014"
     CDS             12689..14134
                     /locus_tag="K8M77_001014"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBI7085525.1"
                     /translation="MVKPNFDIMDPQNDKFKDECGVFGVFANKPIDVASINYYGLYAL
                     QHRGQESSGIAVANGEDIKVHKGLGVLTEAFEAEDLKRLREFNGYISIGHVRYSTAGA
                     KTVENAQPLVSNTKLGPISTAHNGNLVNADVIRSLLEDGGQVFHTSVDSEVITSLVAR
                     GAKKGIERAVIDAISAVKGSFAMVIMTKDKLIGIRDPHGIRPLCLGKFEEGYILTSES
                     CALDTIGAEFVRDIKPGEIVVIDNDGIKSYRYSENTVCQTCAFEYIYFARPDSVIDGL
                     DVQTTRVKQGEILFKEYPIDADIVVAVPDSGIPAAMGYAKASGIPYEVGFVKNRYIGR
                     TFISPSQEIRERAVAVKLNPLKVNVNGKRVILIDDSIVRGTTSKHLIESLRRAGAKEV
                     HFLVASPMVKYPCYFGIDTPYRSQLIGASRSVEEIREMIGCDSLGYLSLEGMYESFEG
                     RRNFCVGCFSGVYPVAAPMEALEDNLERVEN"
     gene            14135..15136
                     /locus_tag="K8M77_001015"
     CDS             14135..15136
                     /locus_tag="K8M77_001015"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBI7085526.1"
                     /translation="MLTYKEAGVNIEEGYRSVKLIKEYAKKTISEYVLNGLGSFAGMV
                     ELPEGYKKPVLVSGTDGVGTKLHIACKKRKFDTVGIDCVAMCVNDILCHGAKPLFFLD
                     YIACGKLEAEVSSDLVKGVAEGCIKSQCSLIGGETAEMPGMYKEGDYDIAGFAVGIVD
                     KDKIINGKDIKSGDKLIGIASSGVHSNGYSLIRKVFKNLDEDFNGKAIWEELLTPTKI
                     YVKPVLSLLEKFNIKGMAHVTGGGFYENLPRMLSKEGLSIVINKNSYDIPEIFKKLME
                     LGVKEEEMYNTFNMGIGFVLCVEEDEVEDVLKELSKQGEKAFEIGYINAGGEGVCIK"
     gene            15124..15738
                     /locus_tag="K8M77_001016"
     CDS             15124..15738
                     /locus_tag="K8M77_001016"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBI7085527.1"
                     /translation="MYKIAVLASGSGSNLQSILDNIDNGNINGEVSLVIGSKEGIFAL
                     ERAEKQGIKTSVVSKKEFEDKTSDEILRLAKENNIDLIVLAGYLSILKGKLLEEYGNR
                     IINIHPSLIPSFCGNKMYGINVHEAAIEKGVKFSGCTVHFVNDEVDGGAIIAQEIVEV
                     NFEDTPESLQKKVLEKEHILLPRIVKYLCEEKIEIHNGKVKILN"
     gene            15751..17256
                     /gene="purH"
                     /locus_tag="K8M77_001017"
     CDS             15751..17256
                     /gene="purH"
                     /locus_tag="K8M77_001017"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBI7085528.1"
                     /translation="MKKRALISVFDKDGVLELAKFLRDRDVEIISSGGTYKYLKENNI
                     EVKEISEITDFPEMLDGRVKTLHPLVHAGILAIRDNKEHMKTLEKREINTIDYVVVNL
                     YPFFEKVRENLSFEEKVEFIDIGGPTMLRAAAKNFKDVVVLSDKKDYEKVMNEIKENN
                     CVSFKLRKTLAGKVFNLMSAYDAAISNFLLEGEEEYPEYLSVSYKKIQDLRYGENPHQ
                     GAAYYSSTEFDGAMNSFEILNGKALSYNNIKDLDIAWKVACEFEETACCALKHNTPCG
                     VAVGENSKEVYLKAYDADPVSIFGGIVAINRKIDKATAEEMVKIFLEVVAAPDFDEDA
                     LEVLRTKKNLRVIKCKNTPQAKNYMVTVDGGILVQGEDNKLANEYKVVTKKEPTEMEL
                     RDMIFGMKVVKYVKSNAIVVVKDGVATGIGGGQVNRIWATKEALERGKGGAVLASDAF
                     FPFRDCVDEAAKNGIKAIIQPGGSIRDEESVEACNEHGISMVFTGVRHFKH"
     gene            17372..18622
                     /gene="purD"
                     /locus_tag="K8M77_001018"
     CDS             17372..18622
                     /gene="purD"
                     /locus_tag="K8M77_001018"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBI7085529.1"
                     /translation="MKILLVGSGGREHALAWKLGKSPKVEKIYVAPGNGGTAKENKCE
                     NIEITKICDLLKFAKNNNIDLTVVGPEEPLINGIVDAFKEEGLKIFGPDKAGAMLEGS
                     KSFSKDFMKKYGVKTADYEVFYDYEEAKSYLENCKYPRVIKADGLAAGKGVVICENKN
                     QAIETLDLFMIDDVFNGAGKKIVVEEFLQGVEASILSVTDGKVILPFLSAKDHKQIFD
                     GNKGKNTGGMGVVSPNKYVTEEVLADFYENILEKTLIGIREEGFDFKGVIFFGIMITE
                     EGAKLLEYNVRMGDPETQSVLSLMESDLVDVILNALDEKLNETTIKWNEGYCVNVVLS
                     SKGYPEAYEKGYEISIDESLKGEYFIAGAKKEGDTLVTSGGRVLSVVGKGETLEEAKE
                     NAYKNIEKVNFEGMYFRKDIGTAK"
BASE COUNT         7532 a         1802 c         3697 g         6020 t
ORIGIN      
        1 ataaaggata aatttcttat tgttaagaaa aatttaaatg aaaattggaa aattattttc
       61 ttttttcttt ttgtaggata taatcatatt actgggaata aatattttat tatttttagt
      121 actttatcaa ttagaatcca aaatttttta tataaataaa aaaaccacac aaacactata
      181 gttaattatc tttaaagaac taatgaaaga gttaaggaga tgatttaaat ggaaaaagac
      241 ataaaaatta atgagggttt aatttcaatt tgtaatgatg attatttaaa tgctatgtct
      301 ttagtaaaag aaaaattaaa tatatactta gaggaaccta aagttttttc aaatgaatat
      361 attttaggaa caccagggtt taaagacaat ttaagagata atttaaagag agcattggca
      421 ataatttttg attttgttgc agtttataaa aaatacgaag taaatgaaaa tataaaaaac
      481 atcttagaaa aggctagaaa tatttacgct atcttagaag aagcacaagg aaatattaat
      541 gaggatgttt taaaatcatt aaaagaaatt agagcaatag aatttaaata atttttatgg
      601 aaaggttagt aacattaaaa gttgctagcc tttttttata tggaaaatta ttttgagatt
      661 tatattgtag acttaaatat taaaaaggtt gtcaataata ggcataaaaa atataatcaa
      721 ggagagtatt aattaaagaa aagtaatttt aatatgaata atagaaataa attagggggc
      781 tagttaaatg tcggttaaag agtcagtact taagattcta gaagaaaata gggagagaag
      841 tatatcagga gaagaattgg ctaaacattt atcagtttct agggcagcta tatggaagac
      901 tataaaatca ttaaggggag agggatacaa tatttctgct gttaccaata gaggatacca
      961 attaacaaag gataatgact taatatcagc agagggaata aaaatatttt taaatcctaa
     1021 ataccgtgaa aactatataa gggtgtataa aactatagat tcaactaatc aagaagctaa
     1081 gaagctttta atggataatg atataccaca tggaaccata ttaattgcag aggaacaaac
     1141 agcaggtagg gggagatttc aaagaaagtt cttttcacct tttaacaaag gaatatatat
     1201 gagtgttatt ttaagaccta acatagaact ttcaaaggcc atacatataa caacctcagc
     1261 agccatatct gtttgtaggg caatagaaac attaactaaa aaaagaccta aaataaagtg
     1321 ggttaatgat atatttttag atgataaaaa aatttgcggt atattaacag aggcaacagg
     1381 aaactttgag agtggaagag ttgaaaatgt tgttgttgga attggagtaa attttaaaaa
     1441 tgattcaaag gatttccctg aagatataaa aaatattgca ggatcaatat ttggaggaga
     1501 aaagccaact attactagaa atcagttaat tgctgagatt ttaaatgaac tttttgaatt
     1561 atgtgaaaat ctagaggata aaagcataat gacagagtat agaattttat cttcggtatt
     1621 aggaaaagaa gtaagttttt taaagaataa taagctgaat aaaggattgg ctattgatat
     1681 tactgatgag ggagctttgg ttgttaagta tgaaaatggt gaaatagatt acttaaattc
     1741 aggtgaaata tcagtaaaaa cattaaataa gtaattttaa taattaagta agagtgttag
     1801 aagtaactat ttctaaatat gtttctaaca ctttttaaat acatactttt agcttaaaaa
     1861 tacagtgtta acaaaaagtt acagaatatg ctacaatatg aatgaaatgt taataggagg
     1921 tgttatataa atgttaaaag aaattatttt cggaatttct tctataaagg gacctacatg
     1981 gcttaaagaa ttcaatgaga ataataaaca attagaagat ttaaaaattc ttagtggaaa
     2041 agtttatagt gagaaaaaga aattaataga tagagatatt atgtacttaa cttatggtat
     2101 tgagggagaa aaaaatgtta gttatgaaat aaaaaattcc tatataccaa tgttagcttt
     2161 acatgatatt aggatagagg acggggaatc agttgcccaa atggattata ttctaataac
     2221 taaaagtttt attttagtct tagaaactaa ggccttaagt ggagatataa ctgtaaatga
     2281 gtcaggagaa ttcataagat attttaaaaa taaagaagga aaagtttata gaaaagaagg
     2341 aatatacagt ccagtagctc aaaatgaaag acctgttaga gtgttaactg agtatttaaa
     2401 gaaaaataaa atgataaaaa aataccctat atattcttgt gtagtaattg caaaatctaa
     2461 aagtgtaata aataagtata aagcaccaaa ggaaataaaa tcacaaatta taaaatatga
     2521 tcaattaaca tcttttataa aagagaaatt aaactatcat aagataaagc aagattttaa
     2581 ggttttagat aaagtagaat attcaatagg taaattttta ttagaaaata ataaacctgt
     2641 agagtttgat tattataaaa aatactcctt aagtgaagag gatttcataa aaaaagagtc
     2701 agaagtatgt agtgaagaaa aatcaaacaa tgataatttt aatgatgagg ttttatttaa
     2761 tagagataat aaatcttatg agggtaaaat attaattaat aaaaataaag aagaactgag
     2821 gagaaaactt aaacaattaa gaataaattt tgcaataaaa gaaaaacttc cttcttattg
     2881 catttttaat gatgagactc taaatgatct tttagataaa atgccaaaga ataaagaaga
     2941 gcttatgaaa gtcaagggat tggctaaggt gaagattaat aaatatgggg aagaaatatt
     3001 aggagtttta aattaaaatg gaatttttaa agaaaaatga gttatggtta ttaataatta
     3061 atgttattat aaataggttt catgttaatg gattaaaaga aaaattttgg tcgtttttac
     3121 ttagtctttt atatgttata attggataat attcaatata ataaggggga ataagtatgc
     3181 cttttaccct agcgcatcca gcagctgtgg ttttttttaa gaataaaaag tttaatctta
     3241 cagctttaat tttagggagt atggctccag atttcatata ttttcttaat tttagacctt
     3301 atggaaattt aggacacgaa atattaggct tttttatatt aaatttgcct ttatgtattt
     3361 tattagcgta tatttttcat aactttataa aatctccact tataagtcat ttgccgagac
     3421 ctttagataa ctggtatggt tacttaagaa cagaacaatt tggcttgcat tcatggaaag
     3481 aagtaattgt tttcatatat tcggctttat taggaatgat tacccatgtt ttgtgggatg
     3541 gatttactca taaaactgga ttctttatgc gtcatatcac ttggcttaga caaaaaatta
     3601 taatatttgg cgcagatgaa atttatatat ataagttagt tcaacataca agtactgtta
     3661 ttggaataat tatagtgtta tttttcttat ataaacttag ggatagtaat actagaattt
     3721 tgtggggaca taaaagtttt aaggataagt ttatatatga tttaatagtt ataacaactt
     3781 caataatagt tttagtttta agttacataa tatttaaata cttaagtatt ccttacggaa
     3841 ttggaaatct tgtggtttca agtataaatg gaatttttat tggaatttta attgcttcct
     3901 ttaaaaaata atttgtaaaa agatatattt ttattagttt attctctagg tttatactaa
     3961 aaataaggaa tattattatc tctatagatt atgtggattt aaagtctaaa tgatatatta
     4021 attttaattg taaagttcac ttcctattct ttaaaatggt ataatatagc tattaaggat
     4081 gaattgaaag ggaagaaact atggatagaa tgcttttatt acaaaatata ttggaagcat
     4141 tagtgtttgt tgtatcaaca attctatttg ctgttttata tggattaatt gataaaaaaa
     4201 taaggttaac tgataaaata tacggagctt ttaaaataga gcctaagtgg gaatggatta
     4261 tccacatagt tatagcttta attttaattg tgttagttca ttattttgga gtttatatat
     4321 ttaagatatg gaatttagca actgtattta taacaggtgc tattgctggg atttgtatat
     4381 ataacatgaa tagaaataat ttttaaaaat aattttaaat aagtaaaaag taacaggtta
     4441 gattgattaa acctgttatt tttttataaa gaattaagtt tttctataaa aaattcttaa
     4501 taaaagtaat aagttgtaaa tgtaagaata aagaaaatat attaacaaaa tattaatgat
     4561 acaactttga attaagaata tggatgattt acacatttgt taataaggga aatttaaaat
     4621 ataattttta ctaaagatgg aggtgagttt tatggttgga gaacaaagag taagagatgt
     4681 atatattcaa tacgataaaa atggagaaac ccctaaatat gaaacaccat tagccgcagg
     4741 agcagacctt tttgcagcag aggatatggt tataagacct tttgaaaagg ttgcaattcc
     4801 actagggtta aagtttgcaa ttcccgataa tatggagctt caaattaggg ctagaagtgg
     4861 actttcttta agaacaaact taaaaatttc aaactcaata ggaactattg atgctgattc
     4921 agttagtgat atatcagtaa ttttagaaaa taattataat atagccaatc ttccttatga
     4981 gatagctaat gatataagta tattagatga tttaaaaaat aattatattg aagtaagatt
     5041 aagcgattat ttagaatcta aaggacatga tataagtgat tatgaggatg aaggaaaaaa
     5101 agcttataaa aagttattaa agacatatat ttatttagat aagaacggaa atccatatgg
     5161 aactatatac ataaaaaaag gaaatagaat tgctcaaatg gttttagcaa aatattataa
     5221 agctaatttt ataaaaacag atgacgtttc taaatttaaa agtgtaaata gaggcggtgg
     5281 atttggtcat acaggctttt aaaattccat tgttttgtag agaaaactta aattataata
     5341 tataaatcgg aatagttatg aaagttttag aatgagatag gaacttattc taaagctttt
     5401 ttaatttcct acttatattt tacttggttt taatttagta tatctttttt atatttagct
     5461 taaataatta ttaagtatat tattacttat ttttataaat ttacttgaaa ttgaaaaaaa
     5521 ttgatagtat aatttagaaa aagtaaatat ttaatcgtaa gtgctagggg tgcattttgt
     5581 atgctgagag gataatatct atctaaccct taaaacctga tgtagttagt actaccgtag
     5641 ggaagcagaa gctattgttt atttatattt atgctttaaa aagttataaa tgtatctata
     5701 taagtgttta ttaagcttgc cttagggcaa gcttttattt tttataaaaa ataggatcct
     5761 atattaattt aactgcgcag ttaaaaaatt aaaataagga gaactaatat gaattataca
     5821 actcaaatgg atgctgctaa aaaaggaata ataacaaagg aaatgcaagt agttgcagaa
     5881 aaagaaggaa ttaatattga aactttaatg aatttaatgg ctgaaggaaa aattgtaata
     5941 ccagctaata aaaatcataa aagtataagt gcagagggtg ttggacaagg attaaaaact
     6001 aaaataaatg ttaacctagg aatttcaaag gattgtgcca atatagaatt agagttagaa
     6061 aaagttaaaa aagcaataga tatgaatgca gaatctataa tggatttaag taattatggt
     6121 aaaacttatg attttagaaa aagacttgta gaagtttcta cggctatgat aggaactgta
     6181 ccaatgtatg atgtagtagg tttctatgat aaagaactta aagatataac tgttgatgaa
     6241 ttttttgatg ttgtagaaaa acatgcaaag gatggagttg actttgttac tatacatgct
     6301 ggattaaata gagaaacaat tgaaactttt agaagaaata aaagacttac taatatagtt
     6361 tctaggggag gatctcttct ttttgcatgg atggaattaa ataatagaga aaatcctttt
     6421 tatgaatatt ttgatagatt attagatata tgtgaaaagt atgatttaac tttaagttta
     6481 ggggatgctt gtagaccagg ttcaatagct gatgcaactg atgctgtaca aatcaaagaa
     6541 ttaattaccc ttggagaact aacaaaaaga gcttgggaaa gaaatgtaca agtaataata
     6601 gagggaccag gtcatatggc aatgaatgaa attgaagcta atgttttatt agagaaaaaa
     6661 ttatgccatg gagcaccatt ttatgtttta ggaccaatag taactgatat tgcaccagga
     6721 tatgatcata taacaagtgc tataggaggg gctatggcgg cttcttatgg agcagatttt
     6781 ctttgttatg taacaccagc agaacattta agacttccta atttagagga tgtaagggaa
     6841 ggaatagttg ccacaaagat agcggctcat gcagctgata tagcaaaagg aatttcaggg
     6901 gcaagagata tagataataa aatgagtgat gctaggaaaa gactagattg ggacgagatg
     6961 ttttctttag caatagatag tgaaaaagca attagataca gaaaagaatc tactcctgaa
     7021 cataaagata gttgtacaat gtgtggaaaa atgtgctcta taagaaatat gaataagatt
     7081 ctagaaggga aggatataaa ccttttaaga gaagactaaa aatattagag atatattaaa
     7141 aaatattatt cgagactttt aatatatctc tatttttatt cttaaatacg aactttaatt
     7201 aataaaaata atataaatac gtctaaaatt atctgaaaaa acgaatgttt aaataatttt
     7261 aaatattgac aatacgtatt taaggtggta atgttaaatt aacgaatgat taatttttaa
     7321 tcattaataa tgttcgtgtt ttgaatgggg gattaaaatg tctggtatta gaatggtatt
     7381 tgtagaaaaa aaagctggtt ttaatgtgga aagtcagata ttacttaagg attttaaaga
     7441 taatttagga attgaagctt tggaggatgt aagagtttta aataaataca tattaggaga
     7501 tatggaagaa gaacagtatg taagaactgt aaatactatt ttgtcagaag ctccagtaga
     7561 tagagtatat gaggaaaatt ttgaaatagg acaagatgaa attgcttttg gtgttgaata
     7621 tttaccagga cagtatgatc aaagagctga ttcagcttca gagtgtataa tgcttcttac
     7681 tgaggaggaa aaaatttctg ttaaatcgtc aaaagtaatt attttaaaag ggaatttaaa
     7741 tgaggaagag ataaagaaaa taaaatctta ttacattaat cctgttgatt caagggaagt
     7801 tagtccactt tcaaaagtct tagaagaaaa cttagaggaa cctaatgatg ttgaggtttt
     7861 agatggcttt ttagatttaa atgaagaggg acttaaaaat ttccataggg ataaatcttt
     7921 agctatgagt ttagaagatt taaaaatgat aagagattat tttaaatcag aggatagaaa
     7981 tccaacaata actgagatta aggttattga tacttattgg tcagatcatt gtagacacac
     8041 aacttttgaa acaataataa aagatgttta tatagaagag ggtaagtatt cagaacctat
     8101 taaaaaggct tatgaagatt ataaaaattc aagagcctat gtttatgggg aaaacttaaa
     8161 taataaagaa gttaagttaa tggatttagc cacaatagcc atgaaggagc taagaaaaag
     8221 aggagaactt gatgatttag atgtttcaga agaaataaat gcttgttcta taaatataga
     8281 aatagaaaca gataagggaa ctgaagaata tcttttaatg tttaaaaatg agacacataa
     8341 tcacccaaca gaaatagagc cttttggagg ggcagccact tgtttaggcg gagctattag
     8401 agatcccctt tcaggaagaa gttatgttta tcaagctatg agagttacag gttcagcaga
     8461 tccaacagtt gaaatttgtg aaactttaaa gggaaaatta cctcaaagaa aaataacttt
     8521 aggagctgct catggatata gctcatatgg aaatcaaata ggccttgcaa ctggacaagt
     8581 atcagaaatt tatcatccaa attatgcggc taagagaatg gaagtaggag cagttatagc
     8641 tgccacgcca aaggaaaatg taataagact aaaaccttca aagggagata ttgtaatact
     8701 tcttggagga agaactggaa gagatggtat tggtggagct acaggttctt caaaggaaca
     8761 cacagaagaa tcaataaatc aatgtggagc agaagttcaa aaaggaaatg ctccaacaga
     8821 gagaaaaatt cagagacttt ttagaaacaa agaagtagct caaatgataa aaagatgtaa
     8881 tgactttgga gctggtggag tatcagtagc aattggagaa ttatgcaggg gaattgatat
     8941 agatttaaat aaagttccta aaaaatatga gggattagat ggaactgaac ttgctatatc
     9001 agaatctcaa gaaagaatgg ctgttgttat ttcctctgaa aatgcagata gatttataaa
     9061 actttcagaa gaggagaatt tagaagctac aattgtggct gaagttacag atactgatag
     9121 attgagaatg aattggaagg ataaaactat agttgatata aagagaagtt tcttagatac
     9181 aaatggggca aaacaagaaa tttctttaaa agtaaaatca ccaagtgctt atccatatga
     9241 aattaaaaac tgtgatgtta aagaggaatg gttaaaatct ttaagaaatt taaatgtttg
     9301 tagtcaaaaa ggattaattg agagatttga ttcaactata ggtggaggaa cagttcttat
     9361 gcctttagga ggaaaatatc aattaacacc agcagagggt atggctgcta aaatccctgt
     9421 cttaggagga gaaagtaaag atgcttcttt aatgacatat ggatttaatc catatttagg
     9481 agtatggagt ccattccaca tggcatttta ttcagttatt gaatcagtaa ctaaaatatc
     9541 tgctatggga ggagattata aaaaagttag attaactttc caagaatatt ttgaaaaatt
     9601 attaagagat gaagaaaaat ggggtaagcc ttttgcagca ttactaggag cttataaggc
     9661 acaaatggat ttaggattac ctgcaatagg tggaaaagat tcaatgtcag gaagttttgg
     9721 agagttaaat gttccaccaa ctttagtttc ctttgcagta ggtcttgaaa aggcttcaag
     9781 aattatttca cctgagttta aaaatatagg atcaacttta gttcttatga aaggtgagaa
     9841 attagaagat ggaactttag aaatagaggg atttaaaaat aacttagaaa aactttatga
     9901 gttaatagga gaagaaaagg tggtttcagc atattctctt aaatttggtg gagtatcgga
     9961 gggaattact aagatgtcac taggaaatag aattggggca acattaaata acatttctaa
    10021 agaagagctt tttggattta attatggaag tttaatatta gaggctaaag aaggggttaa
    10081 ccttgaggag gaatttaagg gaactaatta taaggttata ggaaatacaa tagaggctga
    10141 tgttataaaa tgtgaagaat atgactttga ggtttcttta gaggaattag aaaaatctta
    10201 tgaagaaaaa cttgagtatg tatttaaatc taaaactgaa gataaagaag ggggcttttc
    10261 agacttaata agtaatgata aagatggggc taatatttta gataatggac aaatgcatat
    10321 agaagaaaaa cttaaatcaa agataacaag ggtagaaaag ccaagagtag ttataccagt
    10381 attcccagga actaactgtg agtatgattg tagaagggca tttgaaaaag aaggtgctga
    10441 ggtaagtgaa gttataatca gaaatttaaa taaggaagcc ttaagtgatt caataaatat
    10501 gcttaaaaaa gaaattgaca aatctcaaat aattatgtta ccagggggat tctcagcagg
    10561 agatgagcca gatggatcag ctaaatttat agcaacaata tttagaaatc ctaagataaa
    10621 agattcagtt atgaagcttt taaatgagag agatggatta atacttggaa tttgtaatgg
    10681 cttccaagcc ttaattaagt taggacttct tccatatgga aaaataattg atattgaaga
    10741 agatatggca accttaactt ataacaatat aaatagacat atgtcatcca tagttagaac
    10801 taaaattaca tctaagaaat ctccttggtt taatgaggtt tctcttggag aggtacacag
    10861 tattccaata tcccatggag aaggaagatt tgttgcacca gaatctttaa taaaagagtt
    10921 agtagaaaat gatcaaatag ctactcagta tgtagactta gaaggaaata tggctatgaa
    10981 tatgccatat aatcctaatg gttcatcttt agcaatagaa ggaataacaa gtagagatgg
    11041 aagaattcta ggtaaaatgg gtcattcaga gagaatagga gacaatcttt ataaaaacat
    11101 accaggagaa tttgatcaaa aactatttaa atcaggtgta gattatttta gaaaataagt
    11161 ttataagttt taaattattt cttagataga actaaaggac tagcttaatt aagaaattac
    11221 ggttttatgt tatatattat ctctaaattt ttaagaagag gataaatttc tatcttaaaa
    11281 atttcataag gcatatattt cataatacag gtataaccag ttaagaaatt aaattttcac
    11341 ttgagtttta agctttagat atatatttta gatattattt aaaaatatat tatagaaact
    11401 ttttgaaatt aagttaggtc tttaaggtta tctagaagat aagtattatt aaaactttaa
    11461 aatataggga ggaaatttta atgaaggtag caatattttt tggaagtaaa tcagatatag
    11521 atgttatgaa gggagcagga aatgctctta aagagtttgg aattccttat aatgcttata
    11581 ttttatcagc tcatagggtt ccagaaaaac ttattgaaac tttagaaaaa attgagaaag
    11641 aaggctgtga ggtaataata gctggagcag gattagctgc ccaccttcct ggagttatag
    11701 cttctcatac tatacttcct gtaataggag ttccagtgag agcagccgta gagggaatgg
    11761 atgcattact atcaatagtt caaatgccaa aatcaattcc tgtagccact gttggtataa
    11821 ataatagtta taatgcagga atgttagcag tccaaatgct ttcattaaaa tatccagaac
    11881 taagagaaaa attaatacaa tttagaatcg acatgaaaca taaattcata gcagaaaatg
    11941 gagaaggagt ggaattataa tggtaaatca actagaaatg ttatatgagg gaaaagctaa
    12001 aaaaatatat gcaacagata aagaggatat ggtaattgtt cattataagg atgatgctac
    12061 agctttcaat ggagaaaaaa aggctcaaat agaaagtaag ggagtattaa acaacgaaat
    12121 tactagtctt atttttgaga tgttaaataa ggaaggaata aaaactcact ttgtagagaa
    12181 gttaaatgat agagatcaat tatgtaagaa agttgaaata gttcctttag aagtaatagt
    12241 tagaaatgtg gcagctggaa gtatggctaa gagattaggt cttgaagaag gatatgaatt
    12301 aaagactaca gtttttgaat tatcatataa ggatgattct ttaggagacc cacttataaa
    12361 tgactatcat gctgtaggaa ttggagctac gacttttgaa gaattaaata aaatttatga
    12421 aataactgct aaagtaaatg aaattttaaa agaagctttc aaaaaacaaa atatcaactt
    12481 aatagatttt aaattagagt ttggaagata taagggagag atattattag ctgatgaaat
    12541 ttcaccagat acttgtagat tttgggatgc taaaacagga gaaaaaatgg ataaagacag
    12601 atttagaaga gatatgggta atgtaatcaa tggttataga gaggttttaa atagactaag
    12661 aaattaggta gtaagaggag tagaaaatat ggtgaagcca aattttgata ttatggatcc
    12721 acaaaatgat aaattcaaag atgaatgcgg agtttttgga gttttcgcaa ataaaccaat
    12781 agatgttgct tctataaatt attatggact ttatgctctt caacatagag gacaagaaag
    12841 ttctggtatt gcagtagcaa atggagaaga tataaaagtt cataaggggt taggtgtttt
    12901 aacagaagcc tttgaagctg aagatttaaa aagacttaga gaatttaatg gttatatatc
    12961 aataggacat gttagatatt ctacagctgg agctaaaact gttgaaaatg cacaaccttt
    13021 agttagtaat actaaacttg gtcctatatc tactgctcat aatggtaatt tagttaatgc
    13081 agatgtaata agaagtcttt tagaggatgg aggacaagtt ttccatacta gtgttgattc
    13141 tgaggtgatt acaagcttag ttgctagagg agctaaaaaa gggattgaga gggctgtaat
    13201 tgatgctata tcagcagtta aaggttcttt cgctatggta ataatgacta aggataagct
    13261 aataggtata agagatccgc atggaattag acctttatgc ttaggaaaat ttgaagaagg
    13321 atatatatta acttcagaaa gttgtgcttt agacacaatt ggagcagagt ttgtgagaga
    13381 tataaaacca ggagaaatcg tggttataga taatgatggg attaaaagtt atagatattc
    13441 agaaaatact gtttgtcaaa cctgtgcctt tgaatatata tactttgcaa gaccagattc
    13501 agtaattgat ggtttagatg ttcaaacgac aagagttaag caaggagaaa tattatttaa
    13561 agaatatcca atagatgctg atattgtggt agctgtacca gattcaggaa taccagctgc
    13621 tatgggatat gctaaggctt caggaattcc ttatgaggtt ggatttgtga aaaatagata
    13681 tataggaaga acatttatat caccatcaca agaaattagg gagagagctg tagctgtaaa
    13741 gcttaatcct ttaaaagtta atgtaaatgg aaagagagtt attttaatag acgattctat
    13801 tgttagagga acaacttcca aacatttaat tgaatcttta agaagagctg gtgctaagga
    13861 agttcatttt ctagttgcat caccaatggt taaatatcca tgttattttg gcattgatac
    13921 accatacaga agtcagttaa ttggagcaag tagaagtgta gaagagatta gagaaatgat
    13981 aggttgtgat tctctaggat atttaagttt agaaggaatg tacgaaagtt ttgaaggtag
    14041 aagaaatttc tgtgtaggat gctttagtgg ggtttatcca gttgcagcac ctatggaagc
    14101 tttagaggat aatcttgaaa gggtggagaa ttaaatgcta acttacaaag aggcaggagt
    14161 aaatattgaa gaaggatata gatcagtaaa attaataaaa gaatatgcaa aaaaaacaat
    14221 tagtgaatat gtattaaacg gattaggatc ttttgctggc atggttgaac ttccagaggg
    14281 atataagaaa ccagtgttag tttctggaac agatggagtg ggaacaaaac tccatattgc
    14341 ttgtaagaaa agaaaatttg atactgttgg aattgactgc gttgcaatgt gtgtcaatga
    14401 tattttatgt catggggcta aaccattatt cttcttagat tatatagctt gtggaaaact
    14461 tgaagctgag gtttcttctg atttggtaaa gggagtggct gaaggatgta ttaagtctca
    14521 gtgctcttta attggaggag aaactgctga aatgccaggc atgtataaag aaggagatta
    14581 tgatattgct ggttttgcag taggaatagt agacaaggat aaaataataa atggtaaaga
    14641 tataaagagt ggtgacaagc ttataggaat tgcttcttca ggagttcatt caaatggata
    14701 ttctcttata agaaaggtgt ttaaaaactt agatgaagat tttaatggaa aagctatttg
    14761 ggaagaatta ttaactccta caaagattta tgttaaacct gtattaagtc ttttagaaaa
    14821 atttaatatt aagggtatgg ctcatgtaac tgggggagga ttttatgaaa atcttccaag
    14881 aatgttaagt aaagaaggac ttagtatagt aataaataaa aatagttatg acattccaga
    14941 aatatttaaa aagcttatgg aacttggagt taaagaagag gaaatgtata acactttcaa
    15001 catgggtata gggtttgttt tatgtgttga ggaagatgag gttgaagatg ttttaaaaga
    15061 actttcaaag caaggagaaa aagcttttga gattgggtat ataaatgctg gaggtgaagg
    15121 agtttgtata aaatagctgt attagcttct ggcagtggat ctaaccttca atcaatttta
    15181 gataatatag acaatggaaa tataaatgga gaagtttcct tagttattgg aagtaaggag
    15241 ggtatttttg ccttagaaag ggctgaaaaa cagggtataa aaacatcagt tgtttctaaa
    15301 aaagagtttg aagataaaac ttcagatgaa atcttaagac ttgctaagga aaataatatt
    15361 gatttaattg tactagcagg ctatctttca atattaaaag gaaaactttt agaagagtat
    15421 ggaaatagga taattaatat ccatccatct cttattcctt ctttctgtgg aaataaaatg
    15481 tatggaataa atgtacatga ggcagctata gaaaagggtg tgaaattttc tgggtgtaca
    15541 gttcactttg ttaatgatga ggttgatgga ggagctatta ttgcacaaga aatagttgaa
    15601 gttaattttg aagatactcc agaaagtctt caaaaaaagg ttttagagaa ggagcatata
    15661 ttgctaccta gaattgttaa gtacttatgc gaagaaaaaa tagaaattca taatggaaaa
    15721 gtgaaaatat taaactaggg aggtctttta atgaaaaaga gagctttaat aagtgttttt
    15781 gataaagatg gagttttaga acttgcaaaa tttttaagag atagagatgt agaaataata
    15841 tcaagtggtg gaacttataa atatttaaaa gaaaataaca tagaggttaa agagataagt
    15901 gaaataactg acttcccaga aatgttagat ggaagagtaa aaactctaca tcctttagtt
    15961 catgctggaa tattagcaat aagagataat aaagagcata tgaaaacttt agaaaagaga
    16021 gaaataaata ctatagatta tgtagttgta aatttatatc ctttctttga aaaagttaga
    16081 gagaatttaa gctttgaaga aaaggttgag tttattgata taggtggacc aacaatgtta
    16141 agagcggcag ctaagaattt taaagatgtg gtagttcttt cagataaaaa agattatgaa
    16201 aaagtaatga atgaaattaa agaaaataat tgtgtttcat ttaaattaag aaaaactctt
    16261 gcaggaaaag tatttaactt aatgagtgct tatgatgctg caatttctaa cttcttatta
    16321 gagggagaag aggaatatcc agaatatcta tcagtttcat ataagaaaat tcaagatctt
    16381 agatatggag aaaatccaca tcaaggagct gcttattata gttcaacaga atttgatgga
    16441 gctatgaata gctttgaaat tttaaatgga aaagccttat cttacaataa tataaaggat
    16501 ttagatatag catggaaggt tgcttgtgaa tttgaagaaa cagcttgctg tgcattaaag
    16561 cataatacac cttgtggggt agctgtagga gaaaattcta aagaggttta tttaaaggct
    16621 tatgatgctg atccagtttc aatatttggt ggaatagttg ctataaatag aaagatagat
    16681 aaggctacag cagaagaaat ggttaaaatt ttcttagagg tggtagcagc accagatttt
    16741 gatgaagatg ctttagaagt attaagaact aaaaagaatt taagagttat aaaatgtaag
    16801 aatactcctc aggctaaaaa ttacatggta acagtagatg gtggaatatt agttcaagga
    16861 gaagataata aacttgctaa tgagtataag gttgtaacta aaaaagagcc aacagaaatg
    16921 gaattaagag atatgatctt tggaatgaag gttgtgaaat atgtaaaatc taatgccata
    16981 gtagttgtaa aagatggagt tgccactggt attggtggag gacaagttaa tagaatttgg
    17041 gctactaaag aagctttaga aagaggaaaa ggtggagcag tattagcatc agatgctttc
    17101 ttcccattta gagactgtgt tgacgaagcg gctaaaaatg gaataaaagc aataattcaa
    17161 ccaggtggat ctattagaga tgaggagtct gtagaggctt gtaatgaaca tggaataagc
    17221 atggtattta caggagttag acattttaaa cattagaaat gaatagattt taggaggtga
    17281 agtaggtcat atagttaatt tgaattttaa atagttaatt atataagtta aaactattta
    17341 aactaagttt aatttatatg accttttata tatgaagata cttttagttg gatctggtgg
    17401 aagagagcat gctttagcat ggaaattagg aaaaagtcct aaagtagaaa aaatttatgt
    17461 tgctccagga aatggaggaa cagctaagga aaataaatgt gaaaatatag agattactaa
    17521 gatttgtgat ttattaaaat ttgctaaaaa taataatatt gatttaactg ttgtaggacc
    17581 agaagagcca cttattaatg gtatagttga tgcttttaaa gaggaaggat taaaaatatt
    17641 tggacctgat aaagctggag ctatgcttga aggtagtaaa agtttttcta aggattttat
    17701 gaaaaagtat ggcgttaaga cagctgatta cgaagttttc tatgattatg aagaagctaa
    17761 aagttattta gaaaattgta agtatccaag agtcataaag gctgatggat tagcagctgg
    17821 caagggtgtt gtaatatgtg aaaataaaaa tcaggcaata gaaacactag atttatttat
    17881 gatagatgat gtatttaatg gagcaggtaa gaaaatagta gtagaagaat ttctgcaagg
    17941 ggttgaggca tcaatacttt cagttacaga tggaaaggta atattaccat ttttatctgc
    18001 taaggatcac aaacaaatat ttgatgggaa taagggtaaa aatacaggag gaatgggagt
    18061 tgtttctcct aataaatatg ttactgaaga ggttttagct gacttttatg aaaatatatt
    18121 agagaaaact ttaataggta taagagaaga aggttttgac tttaaaggag ttattttctt
    18181 tggaataatg ataacagaag aaggggctaa acttttagaa tataatgtta gaatgggaga
    18241 tcctgaaaca caaagtgttt tatctttaat ggaaagtgac ttagtagatg taatactaaa
    18301 tgcactagat gaaaaattaa atgaaacaac tattaaatgg aatgaaggat attgcgttaa
    18361 tgttgtttta tcatcaaagg gatacccaga ggcttatgaa aaaggttatg aaataagtat
    18421 agatgaaagt cttaagggag aatactttat agctggagca aaaaaagaag gggatacctt
    18481 agtaactagt ggaggaagag tactttctgt agtaggtaaa ggagaaactt tagaagaagc
    18541 taaagaaaac gcatataaaa atatagaaaa agttaatttt gagggaatgt attttagaaa
    18601 agacataggt actgctaaat agctcttaga aattagcaaa tttaaatata ttataatcat
    18661 aagttatata tttagtattt aatattttta gatagaattt aaaaaataca gttttaattt
    18721 ataactataa ttacaataaa aattaagtac ttaaatataa actatctaag gtttagatga
    18781 gtcaatattt ttttaataag tataaagggg ctttagacag ggttacttta aaatgtgttt
    18841 agaaggtttg atattttttt aataagaaaa attatttgat ataaaataaa aggctttatt
    18901 agaaaaaaat tagatatagg taaacaatct aacagcagtg aaattagttt gttttatcta
    18961 tatctaataa aaatactcta atagagcttt tgtgtttgga cttttttagt tttgtcaaaa
    19021 ataaaaaaac tcttaaacta agtttaagag t
//
LOCUS       DAEVGI010000032        18885 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEVGI010000032 DAEVGI010000000
VERSION     DAEVGI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 18885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18885
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.032"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1656
                     /locus_tag="K8M77_001019"
     CDS             <1..1656
                     /locus_tag="K8M77_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBI7085530.1"
                     /translation="IKINIIFFNFISFISFVFGMFFDLLQVIKNKNFMQEELSAFFNL
                     IEFDCNSEVVVLSNNLKVLYANEKCRSKRISPENRENKTYIDLEKQIKGFLYDKNIIC
                     IESVLRNSKEWKGIIKLNGEDEVVKVNLQKIKKEKNLYYVLRINDITEEYKMEKNLKL
                     EEQRLRGVTENIKDLIFTIDVEGKISYVNKAVIDVLGYSEEELIGKNYYDLLLVESNL
                     NIIDSKYFNEDKILTIDKVRSKKGLVQLESISSRIKDNKNNTLGWVRVARNIEDVREI
                     EILKNKFEEIKQYDKVRSEFFANLSHELRTPINIIYSCIQLLNTSKKNKANFVNLYDK
                     YEKTLKQNCFRMLRLVNNLIDITKIDSGFIKMDFINYDIIKLTEDITMSVIPYVESKN
                     IDIIFDTNCEELEIRCDPDKIERIILNLLSNAIKFTEPGGKIEVSILIDETWVDIRVK
                     DTGIGIPSHMKEFIFGRFIQNDKSLNRNKEGSGIGLSLVKSLVELHEGKVFLRESNES
                     GSEFSILLPNVKLEDDVCENRSLDYKTEVEKISIEFADIYEIY"
     gene            1777..2682
                     /locus_tag="K8M77_001020"
     CDS             1777..2682
                     /locus_tag="K8M77_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI7085531.1"
                     /translation="MKVGVIMGGISSEREISIQSGNSVVHALDKDKYEAIPIVLNEKE
                     DLIEKVKGIDFALLALHGKFGEDGTVQSVLKTLGIPFSGCGPLSSAICMDKDMTKRIL
                     AFGNVRTARWVMVSSVDEIDYEKIENLGYPVFIKPNNGGSSVATTLVESKEAVKDAVL
                     EALKYDTEVMIEEYIKGDEITCPIIDGKMLPVLAIKPKGKFFDIASKYEDGGADEFIV
                     KLNEDLHKEVEKMALETYKLLKCDVYARVDMLVRDNVPYVLEVNTLPGMTKNSLFPKS
                     AAGINMSFEELLDTIIEKSLKVNRE"
     gene            2756..3709
                     /locus_tag="K8M77_001021"
     CDS             2756..3709
                     /locus_tag="K8M77_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBI7085532.1"
                     /translation="MQDKNPLSRFGPDLNEFSRDVNFSILSKNSDFIYLRASGSGTGK
                     LRIDNKFLEFAKECRRLGIPCGAYHFGKPSKDLDSAVIQADQFIDVLQQGFGDGEYGD
                     LFPVLDVETPTDKSLTTTELVNWIDRFRDRFEEKTRRRLMLYTGLFFIVLYDDFKVPG
                     KGYPLSDMPLWIAMYTKIPSNPRVPPNVGGWKRWTVWQFTDEGKLDGVGSPVDLNWGP
                     NSIDGLMPPSAVIGLYAYISGNKIFVTWRANKEDDLNGYNVFVNDNYAATLPRKATKI
                     VIDKSRFYLPKGKPIKISIEAFDITGDFSKERTEYILDNNG"
     gene            3885..5429
                     /locus_tag="K8M77_001022"
     CDS             3885..5429
                     /locus_tag="K8M77_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBI7085533.1"
                     /translation="MKEEKKKRSTGLLKSKKKIIISIVIVLAIIIGSIVAYIVSIQKK
                     VEEWNDKIYPNVYVENVNLSGMTKEKAIDILEKDVKEPVEHKTIKVQAADKSIEIKYS
                     DLSTEYNIDETVNEALNYGKDLNLFEKNNIINGKDKKELNLDFKYDESKLTDYEKKLT
                     EMVNKSAKDATISINGSNISVIEGEEGRIIEENKMVSLLKEAINANPKNNSVVEVPVE
                     VTKPKITKEMLSKIDGIIGSFTTSYTSSDANRSANVEIAAKTVNGTILMPGDTFSYNN
                     TLGERTTAKGYKDGAAYVGNKVVMVTGGGICQVSTTLYRAVLRAGIMPTERHNHSMTT
                     TYSGPSEDATVSWGSLDYQFKNPYDFPIYIQGYTSNKHVTFNVYGNVQGMDGKTYDLQ
                     TVVNETLKPSVKTVDDPNLPEGQKVVEQRPVTGYKSSGYLVTYQNGKEIDKKLIGHDV
                     YKQKDEIIKVGTKKAEQPKQEAPKQEQPATAKPEEPKQEETQPETSQTTTIPQTSNLG
                     QIKPTQ"
     gene            5507..6340
                     /locus_tag="K8M77_001023"
     CDS             5507..6340
                     /locus_tag="K8M77_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBI7085534.1"
                     /translation="MDYKMICIDMDGTLLNSKKVVSEENRIALKKAYEKGVHIIICTG
                     RNPKNAIYFSEFLGVNCAVIANNGAWVIDEDKDIVISKDVLNENQCMDIMNICKEYKV
                     IPSFHSKDSVYWPSRFRKYLCDIILNKKIPEKYRVKNIYVKEKEEWREVFKSNNIGKC
                     IIIELNTSKLKKIRENLIKKGNYEITQSGRYALEVNNKGVSKGRAVEALAEEYKIKRE
                     EIICIGDNENDLSMINYAGLGVAMGNAIDSLKKKADYITESNDKNGVAKVIYEFVLKN
                     D"
     gene            6429..6890
                     /locus_tag="K8M77_001024"
     CDS             6429..6890
                     /locus_tag="K8M77_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HBI7085535.1"
                     /translation="MNLNIVLYQPEIPQNTGNIARTCVLTNSKLHIIKPMGFVIDDKH
                     LKRAGLDYWSQLDLEIHESYEDFMKKYGDKKIYLSTTHAEDHYDEVKYEEEAFIMFGR
                     ESAGVPEDVHNAHHGIKVPMINTTTRSLNLSNTVAIIAYEALRQIGFPNMK"
     gene            6905..7744
                     /locus_tag="K8M77_001025"
     CDS             6905..7744
                     /locus_tag="K8M77_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBI7085536.1"
                     /translation="MDKIKIITDSTCDLSKEIIEKYDIDVMPMLINFGEESYLDGVEI
                     KVDSMMERIEREDLLPTTAQIVPTRFIEKYKGYLEEGYKVISIHISSNMSGTYQSACL
                     AKAELESDDIVVIDSRNVTVGLGLIVLKAARLIESGITLEDLEKEILEYRNHIKSTIA
                     FESLDNLVRGGRLSKGKALFVSALGIKLMLNVLDGEMNVQGKIRGTKKMVKAMIEQFD
                     SIPKKEGEPIILVELENEDIYLPIKEYLENNNIEYLKLPLGCSVAIHSGPKVCALFYV
                     EEY"
     gene            7834..8904
                     /locus_tag="K8M77_001026"
     CDS             7834..8904
                     /locus_tag="K8M77_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7085537.1"
                     /translation="MKLKKTFKVLALLLGVTSIIVGCGANNSSALKKADIIEKQVYNF
                     ALVKDDFSNEENYFADSAVEGLERIKKEYSAEIEVFDTNSSEDYEKRLREAAQNNDLT
                     FASAFKFLESLNNVAKDYPDKNFAIVDTISDLPNVKSIDFKDEEGAFLMGIIAGNATK
                     TNKVGFIGAIKEESIERFSSGFMAGLKVSNEEAYKNIREGNLVQYIGNFNDQKKASDI
                     AKKMYDEGADIIFESAGEAGKGLFRAAKEKGKYAIGADIDWAERFPEYANVILSSMIK
                     NLDEAVYTTAKEVINSDFKAGPNNKMEIGLKDNGIEIAKTTSNLVSKEILDKVEKYRV
                     SIINGEFNVPKTLDEVENFNIK"
     regulatory      8963..9136
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9507..10022
                     /locus_tag="K8M77_001027"
     CDS             9507..10022
                     /locus_tag="K8M77_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI7085538.1"
                     /translation="MNFTKEKKRLDVKMLCFSAILIAISVILANFPIFSSIALDSMPA
                     FVGGIIISPVVGGIIGLLAHLFVALRTGFPLSLPVHILVALEMFVVVYITSIIFNRRK
                     VILAGIVGTLLNGIGFTFITGVFMYFVLRGMNPVDFLKLLGLPLTLASLVNIVIAFIV
                     SKGLKNANIQV"
     gene            10003..10770
                     /locus_tag="K8M77_001028"
     CDS             10003..10770
                     /locus_tag="K8M77_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085539.1"
                     /translation="MQIYKFRDLTILENEKNKIVIACDSCGGIGENEGDFVKAENEIV
                     SYFSARVCLFELLAFRVKPLVIVNNLGMSMNNGGEKIIQGINRAIKEYNAENFFEEKS
                     NLSECVTGSTEDNFKTLQTFLGLTIIGEKEETLKVNFEKGDSICLLGIPKVGQEVLED
                     INNNLGEIVTFKDFKILMDNKDVKDILPIGSKGIIHEISELEKSNKIRINLSYEGDYL
                     RKSAGPATALLFILRNNSLEDIKRKIKTPIMEIGSII"
     gene            11019..12404
                     /locus_tag="K8M77_001029"
     CDS             11019..12404
                     /locus_tag="K8M77_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBI7085540.1"
                     /translation="MLMDFNLNLTQEQKLIMTQQMQLSIKLLQMSTYDLREYIEKEFS
                     ENPVLEAQYEDTKEVSKEQDRLEYKELLKYLESDNYGSQSYGEYDDEEISPFTFISKP
                     ESLTDYLEGQILELPIDEYMRSVCSYMVECLDQKGYLDIKKEELINELDCSEETFNRA
                     LIVIQNLEPAGIGARDLKECLEIQLERKGENDPIVKEIIYNHLDDLADNKYQVIAKDL
                     GITPKKAQDYGDLIKTLEPKPSRGFYTGDEVGFIIPDAEIRKIDGEFLILMNDGVLPM
                     LSVNPLYKAILKDSTNDKEATEYVKEKIEKAMFLIKSIEQRKSTLYKVLQKILEKQKD
                     YFEKGEKYLKPMTLKEIAEKLEMHESTISRAIRDKYILTSMGTIKIKNLFVNSISNKE
                     KSHGEEDVTVINIKKALEEVIKKEDKRKPLSDQAISEILKEKGMVISRRTVAKYREEL
                     GIKSSSKRKRF"
     gene            12632..13666
                     /locus_tag="K8M77_001030"
     CDS             12632..13666
                     /locus_tag="K8M77_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBI7085541.1"
                     /translation="MLEILKLQKIIVPEMVELLVKRYNIIRTIYYNQPIGRRALANNL
                     NLGERIVRTEIGFLKSQNLIQINTPGMSVTQEGEFMLESLKGFIHEIKGLSNLENKLC
                     DMLNIRNVIVVPGDCSEDENTIKELGKAAANYSKNIIKDGYTIAVTGGSTVKEVIDEL
                     PEMANLKNVLVVPARGGMGKKVETQSNTLAANLAKKLNGTYKMLHVPDNISKEVMDAL
                     MKQEDMKELIQNIKNADMLIYGIGQAKKMANKRGVPKDQIIKLLDLGAIGEAFGCYFN
                     DKSEVVYVMPTLGVTINDLRKIKNHIAVAGGKNKAEAILTTVYGNENAVLITDEGAAN
                     EILNFLNRDE"
     gene            13757..14755
                     /gene="gap"
                     /locus_tag="K8M77_001031"
     CDS             13757..14755
                     /gene="gap"
                     /locus_tag="K8M77_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI7085542.1"
                     /translation="MVKVAINGFGRIGRLALRLMIDNPEFEVVAINDLTDAKTLAHLF
                     KYDSAQGRFNGEIEVKEGAFVVNGKEIKVTAKSNPAELPWGELGVDVVLECTGFFASK
                     EKASAHLTAGAKKVVISAPAGNDLPTVVYNVNHDILDGSEDVISGASCTTNCLAPMAK
                     ALNDNFGLNKGFMTTIHAYTNDQNTLDAPHKKGDLRRARAAAANIVPNSTGAAKAIGL
                     VIPELAGKLDGNAQRVPVITGSLTELVCTLDKKVTVEEVNAAMKAASNESFGYTEDPI
                     VSSDVIGISFGSLFDATQTKIMEVDGQQLVKVASWYDNEASYTNQLIRTLKCLVSK"
     gene            14900..16093
                     /locus_tag="K8M77_001032"
     CDS             14900..16093
                     /locus_tag="K8M77_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBI7085543.1"
                     /translation="MNFNKKTIEDVQVKGKKVLVRCDFNVPLKDGVITDENRLNGAMP
                     TIKYLVDNGAQVILCSHMGKPKGEAKPEFSLAPVAKRLSEMLGKEVVFAADDNVVGEN
                     AKKAVAEMKDGDVVLLQNTRYRKEETKNGEELSKELASLAEMFVNDAFGTAHRAHCST
                     VGVTEYLKPAVCGYLIQKELKFLGDAVETPERPFVAILGGAKVSDKINVINNLLEKVD
                     TLIIGGGMAYTFLKAQGYTVGSSLVEEDKVEYAKEMLAKAEEKGVKLLLPVDHRVAKE
                     FKDVEAVVTEDQNIAEGFMGLDIGPKTEAIYAEAIKDAKTVIWNGPMGVFEFENFNKG
                     TIAVAKAMAEADATTIIGGGDSAAAVNILGFGDKMSHISTGGGASLEFLEGKVLPGIA
                     ALNDK"
     gene            16337..17083
                     /locus_tag="K8M77_001033"
     CDS             16337..17083
                     /locus_tag="K8M77_001033"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBI7085544.1"
                     /translation="MRTPIIAGNWKMHYTIDEAVKLVEELKPLVKDAKCEVVVCPTFV
                     CLDAVKKAVEGTNIKVGAQNMHFEEKGAFTGEIAPRMLEAMNIDYVIIGHSERREYFN
                     ETDETCNKKVKAAFAHNLTPILCCGETLEQRENGTTNDVIKAQITADLEGLTKEQAEK
                     VVIAYEPIWAIGTGKTATSDQANETIAAIRAMVAEMFGQEVADKVRIQYGGSVKPNTI
                     AEQMAKSDIDGALVGGASLVAADFAQIVNY"
     gene            17208..18746
                     /locus_tag="K8M77_001034"
     CDS             17208..18746
                     /locus_tag="K8M77_001034"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBI7085545.1"
                     /translation="MSKKPVMLMILDGFGISPNKEGNAVAAANKPNYDRLFNKYPHTE
                     LQASGLEVGLPEGQMGNSEVGHLNIGAGRIIYQELTRITKEIKEGTFFTNKALVKAMD
                     EAKENNTSLHLMGLLSNGGVHSHIDHLKGLLELAKKKGLQKVYVHAFMDGRDVAPSSG
                     KEFIVELENAMKEIGVGEIATISGRYYAMDRDNRWERVELAYNAMVLGEGEKASSAVE
                     AIEKSYHDNKTDEFVLPTVIEEDGHPVSRIKDGDSVIFFNFRPDRAREITRAIVDPEF
                     KGFERKQLHVNFVCMTQYDKTLECVDVAYRPENYTNTLGEYVASKGLNQLRIAETEKY
                     AHVTFFFNGGVEQPNTNEDRALIASPKVATYDLKPEMSAYEVTDELIKRLDEDKYDMI
                     ILNFANPDMVGHTGVQEAAVKAIEAVDECLGKVADKVLEKEGTLFITADHGNAEVMID
                     YSTGKPMTAHTSDPVPFLWISKDAEGKSLKDGGKLADIAPTMLTVMGLEVPSEMTGTC
                     LLNK"
BASE COUNT         7585 a         2004 c         3549 g         5747 t
ORIGIN      
        1 ataaaaataa atattatatt ttttaatttt atatcattta taagctttgt atttggtatg
       61 ttttttgatt tacttcaggt tataaaaaat aaaaatttta tgcaagaaga actaagtgca
      121 ttttttaacc taatagaatt tgattgcaat agtgaagttg tagtacttag taacaattta
      181 aaagttcttt atgcaaatga aaaatgtaga agtaagagaa tatcacctga aaatagagaa
      241 aataaaactt atattgattt agaaaaacaa attaaaggat ttttatatga taaaaatata
      301 atttgtatag aaagtgtgct tagaaattct aaagaatgga aaggtataat aaagttaaat
      361 ggagaggatg aagttgtaaa ggttaatctt caaaagataa aaaaagaaaa aaatctttat
      421 tatgtactta gaattaatga tataacagaa gaatacaaaa tggagaaaaa tttaaaatta
      481 gaggagcaaa ggcttagagg agttactgaa aatataaaag atttaatatt tacaattgat
      541 gttgaaggaa aaataagcta tgtaaacaaa gcagtaatag atgttttagg atatagtgaa
      601 gaagaattaa tagggaaaaa ttattatgat ttattattag ttgaatctaa tttgaatata
      661 atagacagca aatattttaa tgaagataag attttaacaa tagataaggt aagatctaaa
      721 aaagggttgg ttcaattaga atctatttct agtagaatta aggataataa aaataatacc
      781 ttaggatggg taagagttgc tagaaatata gaggatgtaa gagaaataga aatattaaag
      841 aataaatttg aagaaataaa gcaatacgac aaggttagaa gtgagttttt tgcaaattta
      901 tctcatgagc ttagaactcc tattaacata atatattcat gcatacagct tttaaacact
      961 agtaaaaaga ataaggcaaa ctttgttaat ttatatgata agtatgaaaa aactttaaaa
     1021 caaaattgtt ttaggatgtt aaggcttgta aataatctta tagatataac aaaaatagat
     1081 tctggtttta ttaagatgga ctttattaat tatgacataa taaagcttac agaagatata
     1141 actatgtctg taattcctta tgtagaatct aagaatatag atataatctt tgatactaat
     1201 tgtgaggaat tagagataag gtgtgaccca gataaaattg agagaataat tttaaattta
     1261 ttatctaatg ccataaaatt tacagagcca ggtggaaaaa tagaagttag tattttgata
     1321 gatgaaactt gggtagatat aagagttaag gatacaggta taggaattcc atcacatatg
     1381 aaagaattta tttttggaag atttatacaa aatgataaat ccttaaatag aaataaagaa
     1441 ggaagtggaa tagggttatc cttagttaaa tctttagtgg aattacacga aggaaaagtt
     1501 ttcttaagag aaagtaatga atcaggtagt gaattttcaa tattactacc taatgtgaaa
     1561 ttggaggatg atgtttgcga aaatagaagc ttagattata aaacagaggt tgaaaaaata
     1621 tcaatagagt ttgctgatat ttatgaaata tattagaaga tttaataagt ttaaatgatt
     1681 tcataaactt tctattttaa aaagataatg atataatatt aaaggcttgt ttaaaaaaag
     1741 acaagtcctt aattatttct atttggaggt aaaaatatga aagttggagt aataatggga
     1801 ggcatttctt cagaaagaga gatatcaata caaagtggta attctgtagt tcatgcctta
     1861 gataaagata aatatgaagc tattcctata gttttaaatg aaaaggaaga cttaatagaa
     1921 aaggttaaag gaattgattt tgctctttta gctttacatg gaaaatttgg tgaagatgga
     1981 actgttcaat ctgttcttaa aactttagga ataccatttt caggatgtgg tccattaagt
     2041 agtgctatat gtatggataa ggacatgaca aagagaattt tagcctttgg aaatgttaga
     2101 acagcaagat gggtaatggt ttcatcagta gatgaaatag actatgaaaa aatagaaaat
     2161 ttaggttatc cagtatttat aaaacctaat aatgggggat catcagttgc tacaacttta
     2221 gttgaatcta aagaagcagt taaggatgct gtattagaag ccttaaaata tgatacagaa
     2281 gtaatgattg aagaatacat taaaggtgat gagataactt gtcctataat tgatgggaaa
     2341 atgttacctg ttttagctat aaaaccaaag gggaaattct ttgatatagc ttcaaagtat
     2401 gaagatggag gagcagatga atttatagtt aaattaaatg aagatcttca taaagaagta
     2461 gaaaaaatgg ccttagaaac ttacaagtta ttaaaatgtg acgtatatgc tagagtagac
     2521 atgctagtaa gggataatgt tccatatgta ttagaagtaa atacattacc tggtatgaca
     2581 aagaatagtt tattccctaa aagtgcagca ggaataaata tgagttttga agaactttta
     2641 gatacaataa tagaaaaatc tttaaaggtt aatagagaat aattttattt agtaacaatt
     2701 ttccctttaa tattatattt tatatagtaa gaactacttt tcagggagag tattaatgca
     2761 agataaaaat ccattaagta gatttggtcc agatttaaat gaatttagta gagatgttaa
     2821 tttttcaata ctatctaaaa attcagattt tatttattta agagcttcag gctctggaac
     2881 aggtaaacta agaattgata ataagttttt agagtttgca aaagaatgca gaagattagg
     2941 aattccatgt ggtgcatatc attttggtaa accttctaag gatttagata gtgctgttat
     3001 tcaagcagat caatttattg atgttttgca acaaggcttt ggagatggcg agtatggaga
     3061 tttatttccg gtattagatg tggaaactcc tacggataaa tctttaacta ctacagaact
     3121 tgtaaattgg attgatagat ttagagatag atttgaagag aaaaccagaa gacgcttaat
     3181 gctatataca ggattattct tcatagttct atatgatgat tttaaggttc caggaaaagg
     3241 atacccttta agtgatatgc ctttatggat tgctatgtac actaaaattc cttctaatcc
     3301 aagggttcca ccaaatgttg gaggatggaa gagatggact gtgtggcaat ttactgatga
     3361 aggaaaatta gatggagttg gaagtccagt agatttaaac tgggggccaa atagcataga
     3421 tggtttaatg ccaccttctg cagttattgg actatacgca tatatatctg gaaataaaat
     3481 atttgttact tggagagcta ataaagaaga tgatttaaat ggatataatg tttttgtaaa
     3541 tgataattat gcagcaacat taccgagaaa agcaactaaa attgtaattg ataaaagtag
     3601 attttatctt ccaaaaggaa agccaataaa aatatcaata gaagcatttg atattactgg
     3661 agacttttca aaagaaagaa ctgaatatat tttagataat aatggttaat tcctaggata
     3721 gattattttt atgttaaata gagttaaaat tttttagctc tatttaattt ttctccaaaa
     3781 gctatgaaaa ttttgttata ataataaatg ttattatatt gtaataattt ttagtaatat
     3841 ttagattata gtttttttag aaggttttag agaggagagt aattgtgaaa gaagaaaaga
     3901 agaaaagaag tacaggctta cttaaaagca aaaagaaaat aataatatca atagttattg
     3961 tattagcaat tataataggt tctattgttg catatatagt tagtattcag aaaaaggttg
     4021 aagagtggaa tgataagata tatcctaacg tatatgttga aaatgtaaat ttatcaggaa
     4081 tgacaaagga aaaggccatt gatattttag agaaggatgt aaaagaacct gtagaacata
     4141 aaactataaa agttcaagcg gcagataaaa gtattgaaat aaaatattct gatttatcaa
     4201 cagaatacaa tatagatgaa accgttaatg aagctcttaa ttatggtaaa gatttaaatc
     4261 tttttgagaa aaataacatt ataaatggaa aagataaaaa agaattaaat ttagacttta
     4321 aatatgatga gtctaagtta acagattatg agaaaaaact tactgaaatg gtaaataaaa
     4381 gtgctaaaga tgctaccata agtataaatg gtagtaatat aagtgtaata gaaggcgaag
     4441 aaggaagaat aatagaagaa aataaaatgg tttctttatt aaaagaagct ataaatgcaa
     4501 atccgaagaa taattcagtt gtggaagtac ctgtagaggt tacaaaaccg aaaataacta
     4561 aggaaatgtt atcaaaaata gacggcataa taggaagctt taccacaagt tatacaagct
     4621 cagatgctaa tagaagtgct aatgttgaaa ttgcagctaa aacagttaat ggaactattt
     4681 taatgccagg agatacattt agttataata atactttagg agaaagaacc acagctaagg
     4741 ggtataaaga tggagcggct tacgtaggaa ataaagtagt aatggttaca ggtggaggaa
     4801 tatgtcaagt ttctacaacg ttatatagag ctgttttaag agctggaata atgccaacag
     4861 agagacataa tcatagtatg acaactactt attcaggacc aagtgaagat gctacagttt
     4921 catggggatc tttagactat caatttaaaa acccttatga ttttccaata tatatacaag
     4981 gatatacaag caataaacat gtaacattta atgtatatgg aaatgtacaa ggtatggatg
     5041 gaaaaactta tgacttacaa actgtagtaa atgaaactct aaaaccatca gttaaaacag
     5101 ttgatgatcc taatttgcca gagggacaaa aagttgttga gcaaagacca gttacaggat
     5161 ataagtcatc aggatattta gtaacttatc aaaatggaaa agaaatagat aagaaattaa
     5221 taggacatga tgtatataaa caaaaggatg aaattataaa ggttggaaca aaaaaagctg
     5281 aacaaccaaa gcaagaagca ccaaaacaag aacagccagc tactgcaaag ccagaggaac
     5341 caaaacaaga ggaaactcag ccagaaacat cacaaactac tacaatacct caaacatcta
     5401 atttagggca aataaaaccg actcagtaaa caagtaaatt taatattata aaaaggggag
     5461 aggttaataa attaactctc tccttttatt tttaggagga gaatttatgg attataaaat
     5521 gatatgtata gatatggatg gtacattatt aaattcgaag aaagttgtaa gtgaagaaaa
     5581 tagaattgca ttaaaaaaag cttatgaaaa gggagtgcac ataataatat gtacagggag
     5641 aaatcctaaa aatgcaatat atttttcaga atttttagga gtaaactgtg ctgtaatagc
     5701 taataatgga gcttgggtaa ttgatgaaga taaagatatt gtaatatcaa aggatgtttt
     5761 aaatgaaaat caatgtatgg atattatgaa catatgtaaa gagtataaag tgattcctag
     5821 ttttcattca aaggatagtg tatattggcc atcaagattt agaaaatatt tatgtgatat
     5881 tatattgaat aaaaaaatcc ctgagaaata tagagttaag aatatttatg taaaagaaaa
     5941 ggaagagtgg agagaggtct ttaaaagcaa taatataggt aagtgtatca taatagaatt
     6001 aaataccagt aagctaaaga aaataagaga aaacttaatt aaaaaaggta actatgaaat
     6061 tactcaatca ggaagatatg cattagaagt aaataataaa ggggtatcaa aaggaagagc
     6121 tgtagaggct ttagcagagg aatataagat aaaaagagaa gagattatat gtataggtga
     6181 taatgaaaat gatttatcta tgattaatta tgcaggttta ggtgttgcta tgggaaatgc
     6241 catagattct ttaaagaaaa aagcagatta tataactgaa agtaatgata aaaatggggt
     6301 agcaaaggta atatatgaat ttgtattaaa gaatgattaa cttgcatttt atttcattag
     6361 ataatatcat ataattgtga cttagtttaa aaatttataa ttttttacat ttatttggag
     6421 ggacaaaggt gaatcttaat atagtattat atcaaccaga aataccacaa aacactggta
     6481 atatagcaag aacatgtgtt ttaacaaatt caaagcttca tataataaaa cctatgggct
     6541 ttgttataga tgataagcat ttaaaaagag ctggattaga ttattggagt caattagatc
     6601 ttgaaattca tgaaagttat gaagatttta tgaaaaagta tggagataag aagatttact
     6661 tatcaacaac tcatgcagag gatcattatg atgaagtaaa gtatgaagag gaagctttta
     6721 taatgtttgg aagagagtca gctggagttc cagaggatgt tcataatgct caccatggaa
     6781 taaaagtacc aatgataaac acaactacta gaagtttgaa tttatcaaac acagtagcta
     6841 ttatagctta tgaagcttta agacaaattg gatttccaaa catgaaataa gggggaaata
     6901 tattttggat aaaataaaga tcataacaga tagtacctgt gacttatcta aggagattat
     6961 agaaaagtat gatatagacg taatgcctat gttaataaat tttggagaag agagctattt
     7021 agatggagtc gaaataaaag tagactctat gatggagaga attgaaagag aagatcttct
     7081 tccaactaca gctcaaatag ttccaactag gtttatagag aaatataagg gttacctaga
     7141 agagggatat aaagttatat ctatacatat atcatctaat atgagtggta cttatcaatc
     7201 agcatgttta gctaaggctg agctagaaag tgatgatata gttgtaatag attcaagaaa
     7261 tgtaacagtt ggacttggat taattgtatt aaaagcagct agattaatag aatcaggaat
     7321 aactttagag gatttagaaa aagaaatttt agaatacagg aatcatatta aaagtactat
     7381 agcctttgaa agtttggaca acttagtaag agggggaaga ctttcaaaag gaaaagcttt
     7441 atttgttagt gccttaggca taaaacttat gcttaatgta ctagatgggg aaatgaatgt
     7501 tcaaggaaaa ataagaggaa ctaaaaagat ggtaaaggct atgattgaac aatttgattc
     7561 aatccctaaa aaagaaggag agcctattat actagttgaa cttgaaaatg aggacattta
     7621 cttacctata aaggaatatt tagaaaataa taatattgaa tatttaaaac ttcctctagg
     7681 atgttcagta gcaattcatt ctggtcctaa agtttgtgca ttattttatg tagaagaata
     7741 ttaaaagaaa ctcctaaggg agtttctttt tttacataaa ttttcttatt taaagaaata
     7801 ataattgtga ctaaaaaaat aggaggacta aaaatgaaat taaaaaaaac gtttaaagtt
     7861 ttagctttgc ttttaggagt aacttcaata attgttggat gtggtgcaaa taattcctcg
     7921 gctttaaaaa aagcagatat tatagaaaaa caagtttata attttgcctt agtaaaagat
     7981 gacttttcta atgaggaaaa ttattttgct gattcagctg tagaaggatt agagagaata
     8041 aaaaaagaat atagtgctga aatagaagtt tttgatacta attcatcaga ggattatgag
     8101 aagagactta gagaagcagc tcaaaacaat gacttaactt ttgcttcagc ctttaaattt
     8161 ttagagagtt taaataatgt agctaaggat tatccagata aaaactttgc tatagtagat
     8221 actatatcag accttccaaa tgtaaaatca atagatttta aggatgaaga gggtgcattt
     8281 ctaatgggta taatagcagg gaatgctact aaaacaaata aggtaggatt tataggtgca
     8341 ataaaggaag aatcaataga aagattttca tcaggattta tggctggact aaaggtttca
     8401 aatgaggaag cctataaaaa cataagagaa ggaaatttag ttcagtatat aggaaacttt
     8461 aatgatcaaa agaaagcaag tgatatagct aaaaaaatgt atgatgaagg ggcagatata
     8521 atatttgaat cagcaggaga agctggaaaa ggacttttta gagctgctaa ggaaaaggga
     8581 aagtatgcca taggagctga tattgattgg gcagaaagat ttccagagta tgctaatgtt
     8641 atattatcat caatgataaa gaatctagat gaagcagtat atactacagc aaaagaagtt
     8701 ataaatagtg attttaaagc tggtccaaat aacaaaatgg aaataggcct taaggataat
     8761 gggattgaga tagctaagac aacaagtaat ttagtttcta aggaaatttt agataaggta
     8821 gaaaagtata gagtttctat tataaatgga gaatttaatg taccaaagac cttagatgag
     8881 gttgaaaatt ttaatattaa gtaaaagtaa tataaatatg aaggttgaca aaaggtatat
     8941 aagtgtgtaa ttataaagtg aaaatgaaat aagagcatta ggtgtttagt aacttaatag
     9001 ggaaagttaa aaactgcagc ccccgctact gttgataagg acgagaacaa aaagccactg
     9061 tgataaatag tcatggaaag gattgtttta ggatgattta ttagccagga gacctgccta
     9121 gtatgctatt cttattgttt cggagggaag cattatataa gtttaaaagt atgtaaatta
     9181 ttttaactgg tggggatagt tatccgtacc agttttttta ttttgaaaaa atatttacag
     9241 aaagaatgag tataagcttt cctaaaaatt aattagaaat agaaatttct aactaaaaaa
     9301 ttttaactct atttaattaa ggatcaatta ttctaaggtt atacaaagat attatgtttt
     9361 agtataatta tgaaaaattg aaattataat ttatgttttg ttgggtatga aattttattt
     9421 aatattaaaa gtataacttg ctagttaata gataccttat aatgtagagg aaggaaaata
     9481 atattaatta ggaggataaa ataattatga attttactaa ggaaaaaaag agattagatg
     9541 ttaagatgtt atgtttttca gctatactta tagcaataag tgttatatta gctaactttc
     9601 ctatattctc tagtatagca ttagattcta tgccagcttt tgttggaggt ataataatat
     9661 cacctgtagt tggaggaata ataggacttt tagctcattt atttgtggct ttaagaacag
     9721 gatttccttt aagtttacca gtacatattc tagtagcatt agaaatgttt gttgttgtat
     9781 acataacatc aataatattt aacagaagaa aagtaatttt agcaggaatt gttggtactc
     9841 ttttaaatgg aataggattt acttttataa caggagtatt tatgtatttt gttttaagag
     9901 gaatgaatcc tgtagacttt ttaaaacttt taggattacc acttacttta gcaagcttag
     9961 ttaatatagt tatagcattt atagtaagta agggattaaa aaatgcaaat atacaagttt
    10021 agagacttaa ctatattaga aaatgaaaaa aataaaatag taatagcttg cgatagttgt
    10081 ggtggaattg gtgaaaatga aggagacttt gtaaaagccg aaaatgaaat tgtatcatat
    10141 ttctcagcta gagtatgtct ttttgagctt ttagctttta gggtaaaacc attagtaata
    10201 gttaataatt tgggtatgtc tatgaataat gggggagaaa aaataataca aggtattaat
    10261 agagccataa aagagtataa tgctgagaac ttttttgaag aaaaatctaa tttatcagag
    10321 tgtgtaacag gaagcacaga ggataacttt aaaactcttc aaaccttttt aggattaaca
    10381 ataataggag aaaaagaaga aacattaaag gttaattttg aaaagggaga ttccatttgt
    10441 ttattaggaa tacctaaagt tggacaagaa gttttagagg atataaataa taatcttgga
    10501 gaaattgtta cttttaaaga ctttaagatt ttaatggata ataaggatgt taaagatata
    10561 ttacctattg ggtcaaaggg gattattcat gaaataagtg aattagaaaa gagtaataaa
    10621 ataaggataa acttatccta tgaaggtgat tatttaagaa aatcagcagg acctgctaca
    10681 gctttattat ttattctaag aaataattct ttagaagata taaagagaaa aataaagact
    10741 cctataatgg aaataggaag tattatttaa attaaatata aaaatagtaa gggcatttat
    10801 caaagcttaa taaaacttat gaaatattct tagaatcatt agatttctca aagaagtttt
    10861 gtatggttta ctaaaagtta ttttggtatg tgcctttttt tataattagc taaaatttta
    10921 aattattgaa aaataatttt atttgatgga taattatata agaaaggtta aaagataaaa
    10981 gtgaatagat tagaattaga taattttagg tgatatatat gttaatggat tttaatttga
    11041 atttaactca agagcaaaag cttattatga cccagcaaat gcaactttca ataaaacttt
    11101 tacaaatgtc aacatatgat ttaagagaat atattgaaaa agaattttca gaaaatcctg
    11161 tattagaagc tcagtatgag gatactaaag aggtgtcaaa agagcaagat agattagaat
    11221 ataaagagtt attaaaatac ttagagtcag ataattatgg ttctcaaagt tatggtgaat
    11281 atgatgatga agaaatatca ccttttactt tcataagtaa gccagaatct ttaacagatt
    11341 atttagaagg gcaaatatta gaactaccca tagacgaata tatgagaagt gtatgtagtt
    11401 atatggttga gtgtttagat caaaagggat atttagatat aaaaaaagaa gaattaatta
    11461 atgagctaga ttgttctgaa gagactttta atagggcttt aatagttatt caaaacttag
    11521 aacctgctgg tataggagca agagatttaa aggaatgctt agaaattcag ttagaaagaa
    11581 aaggtgaaaa tgaccctata gttaaagaga ttatatataa tcatttagat gatttagcag
    11641 ataataaata tcaagttatt gcaaaggatt taggaattac tcctaaaaaa gcacaagatt
    11701 atggagattt aataaaaact ttagaaccaa aaccatcaag aggcttttac actggtgacg
    11761 aagtagggtt tataattcct gatgcagaaa tacgaaagat agatggagaa ttcctcatat
    11821 taatgaatga tggagtttta cctatgcttt cagttaatcc tttatataaa gctatattaa
    11881 aagatagtac taatgataaa gaggctacag agtatgtaaa ggaaaaaata gaaaaagcta
    11941 tgtttttaat taaaagtata gagcaaagaa aaagtacttt atacaaagtt ctgcaaaaaa
    12001 tacttgaaaa gcaaaaggat tattttgaaa agggagagaa atatttaaag cctatgactt
    12061 taaaagaaat agctgagaaa ctagaaatgc atgaatcaac tatttcaaga gctataagag
    12121 ataagtatat tttaacttct atgggaacaa taaaaataaa gaatctcttt gtaaactcaa
    12181 taagtaataa agaaaaaagt catggagaag aagatgttac agttataaat ataaagaaag
    12241 ctttagaaga agtaattaag aaagaggata aaaggaagcc cttatcagat caagccataa
    12301 gcgaaatttt aaaagaaaaa ggaatggtta tttcaagaag aactgtggca aaatacagag
    12361 aagagttagg cataaagtca tctagcaaga gaaaaagatt ttaaaataga aaagaatttc
    12421 tatcttgttg caaattaggt tataaagtgc ttattttgag tgaaaaatat agagagactg
    12481 ggtaatctct ctatattttt ttatattttt taaaaaaata agaaaaaatt ttctaaaaac
    12541 cctttaattt tttcttaata tgttttataa taaacgtgcg ggacatttga gaagcgagtg
    12601 ggacttagag tgtccaaagg agttgttaaa tttgctagag attttaaaat tgcaaaagat
    12661 aatagttcct gaaatggtag aactattagt taaaagatac aatataatca gaacaattta
    12721 ttataatcag ccaataggta gaagagcttt ggcaaacaat ttaaatttag gtgaaagaat
    12781 tgtaagaaca gaaataggtt ttttaaaatc tcaaaacctt atacaaataa acactccagg
    12841 tatgtctgta acccaagaag gagagttcat gttagaatct ttaaaaggat ttatacatga
    12901 aataaaaggg ctatctaatc ttgagaacaa attatgtgat atgcttaata taagaaatgt
    12961 aattgttgtt ccaggagatt gcagtgagga tgaaaacact ataaaagagc ttggaaaagc
    13021 cgctgcaaat tattctaaga atatcattaa agatggctat actattgctg ttactggtgg
    13081 aagtactgta aaggaagtta tagacgaatt accagaaatg gcaaacctaa aaaatgtgtt
    13141 agtggtgcct gcaagaggtg gtatgggtaa gaaggttgag acgcagtcga atacattagc
    13201 tgcaaattta gctaaaaagt taaatggaac ctataaaatg cttcatgttc ctgataatat
    13261 aagcaaagaa gtaatggatg ctttaatgaa gcaggaagat atgaaggaac ttatacaaaa
    13321 tattaaaaat gcagatatgc taatatatgg tataggtcag gctaaaaaga tggctaataa
    13381 aagaggagtg cctaaggatc aaataataaa gttattagat ttaggtgcta taggagaagc
    13441 ttttggatgc tactttaatg ataagtcaga agttgtttat gtaatgccaa ctttaggggt
    13501 tacaataaat gatcttagaa aaataaaaaa tcacatagct gtagctggtg gtaagaataa
    13561 agcagaagct atattaacta ctgtttatgg caatgaaaat gccgttctta ttactgatga
    13621 aggagcagct aatgaaattt tgaatttttt aaatagagat gaataaaaaa atatgaaatc
    13681 tctaaaaata actttgttta aaatattttt aaatatttta tatataaaaa acaacatctt
    13741 ttaggaggta tttataatgg taaaagtagc tattaacgga tttggaagaa taggaagatt
    13801 agcgttaaga ttaatgatcg acaaccctga gtttgaggtt gtagcaatca acgacttaac
    13861 tgatgctaag actttagcac acttattcaa atacgattca gcacaaggaa gattcaatgg
    13921 tgaaatagaa gttaaagaag gagctttcgt agttaacgga aaagaaatca aagtaactgc
    13981 taaaagcaac cctgctgaat taccatgggg agaattagga gtagacgtag tattagagtg
    14041 tactggattc ttcgcatcaa aagagaaagc ttcagctcac ttaactgctg gtgctaaaaa
    14101 agttgttatc tcagctcctg ctggaaacga cctaccaaca gttgtttaca acgtaaacca
    14161 cgatatatta gatggaagcg aagatgttat ctcaggtgct tcatgtacta caaactgctt
    14221 agctccaatg gctaaagctt taaatgataa cttcggatta aacaaaggtt tcatgactac
    14281 aatccatgct tacactaatg accaaaacac tttagatgct ccacacaaaa aaggagactt
    14341 aagaagagct agagctgctg ctgctaacat agttccaaac tcaactggag ctgctaaagc
    14401 tatcggttta gttatcccag aattagctgg taaattagac ggaaacgctc aaagagtacc
    14461 tgtaataact ggttcattaa ctgagttagt ttgtacttta gataaaaaag taacagtaga
    14521 agaagtaaac gctgctatga aagctgcttc aaacgaatca ttcggataca ctgaagatcc
    14581 aatagtatca tcagacgtta tcggaataag cttcggatca ttattcgatg ctactcaaac
    14641 aaaaataatg gaagttgacg gacaacaatt agttaaagtt gcttcatggt atgacaacga
    14701 agcttcatac actaaccaat taatcagaac tttaaaatgc ttagtttcta agtaattttt
    14761 taaaattaaa tatttagact gattatataa aagggtctgg ttttatagtt aaagctatga
    14821 ggccagacta ctttaacata taaatgattt ttgtttatat taaaggtaat tataaaatat
    14881 tatttaagag gtgaatataa tgaacttcaa taaaaagact atagaagacg ttcaagtaaa
    14941 aggtaaaaaa gtattagtaa gatgcgactt taacgttcca ttaaaagatg gtgttataac
    15001 tgatgaaaac agattaaatg gagctatgcc aactataaaa tacttagttg ataatggcgc
    15061 tcaagtaatc ctttgctcac acatgggtaa accaaaagga gaagctaaac cagaattctc
    15121 attagcacct gtagctaaaa gattaagcga aatgcttggt aaagaagtag tgtttgctgc
    15181 tgacgacaac gttgttggag aaaatgctaa aaaagctgta gctgaaatga aagatggaga
    15241 cgtagtttta cttcaaaata caagatatag aaaagaagaa actaaaaatg gtgaagagtt
    15301 atcaaaagaa ttagctagct tagctgaaat gtttgttaac gatgcattcg gtactgctca
    15361 cagagctcac tgctcaacag ttggagtaac tgaatactta aaaccagcag tatgtggata
    15421 cttaatccaa aaagagctta agttcttagg cgatgctgtg gaaactccag aaagaccatt
    15481 cgttgctatc ttaggtggag ctaaagtttc agataagata aacgttataa acaacctttt
    15541 agaaaaagtt gatactttaa taataggtgg aggaatggct tacacattct taaaagctca
    15601 aggatatact gtaggaagtt cattagtaga agaagataaa gttgaatacg ctaaagaaat
    15661 gttagctaaa gctgaggaaa aaggggttaa attattatta cctgtggacc acagagtagc
    15721 taaagaattc aaagatgttg aagctgtagt tacagaagat caaaacatcg ctgaaggatt
    15781 catgggatta gatatcggac caaaaactga agctatctac gctgaagcta taaaagatgc
    15841 taaaacagtt atctggaacg gaccaatggg agtattcgaa ttcgaaaact tcaacaaagg
    15901 aacaatagct gttgctaaag ctatggctga agcagatgct actactataa tcggtggtgg
    15961 agattcagct gctgctgtaa acatcttagg attcggtgac aaaatgtctc acatctcaac
    16021 tggtggtgga gcttcattag aattcttaga aggaaaagta ttaccaggaa tagctgcttt
    16081 aaatgataaa taatattata aaccttaaag tagatattta gattgaagta taaagattgt
    16141 tgttttataa aagaataaga ctaagttcaa tgaaatttat ttttcattga atcttagcct
    16201 tattatacta aaagttaaga aaatcttagg cttattattt aatattttca aagcttttag
    16261 gaagataata tatagtaggg gattgagcct tatatatatt atttaatatt taaaatatta
    16321 atagaggtga atatagatga gaactccaat aatcgcagga aactggaaaa tgcactatac
    16381 aatagatgag gctgttaaat tagttgaaga attaaagcca ttagttaaag atgctaaatg
    16441 tgaagttgtt gtttgcccaa cttttgtttg cttagatgca gttaaaaaag ctgttgaagg
    16501 aacaaacata aaagtaggtg ctcaaaacat gcactttgaa gaaaaaggag cattcactgg
    16561 agaaatcgct ccaagaatgt tagaagctat gaacatagat tatgttataa ttggacatag
    16621 tgaaagaaga gaatacttca acgaaacaga tgaaacttgc aacaaaaaag ttaaagctgc
    16681 tttcgcacac aacttaactc caatcctttg ctgtggagaa actttagagc aaagagaaaa
    16741 cggaactact aatgacgtta taaaagctca aataactgct gatttagaag gattaactaa
    16801 agagcaagct gagaaagttg ttatagctta cgaaccaatc tgggctatag gaactggaaa
    16861 aactgctact tcagaccaag ctaatgaaac aatagctgct ataagagcta tggttgctga
    16921 aatgttcgga caagaagttg ctgataaagt aagaatccaa tacggtggat cagttaagcc
    16981 taatacaata gcagagcaaa tggctaaatc agacatcgat ggtgctttag ttggtggagc
    17041 tagcttagtt gcagctgact ttgctcaaat cgtaaactac taataaatat tagtaattaa
    17101 atagtagcaa taggcaagta ataccaattg tctattgcta ttgtttttta attaggtaca
    17161 cattataata aaagtataaa tagaaattta gtttttggag ggttattatg agtaaaaaac
    17221 ctgtaatgtt aatgatatta gacggatttg gaatttctcc aaacaaagaa ggtaatgcag
    17281 tagctgctgc taataaacca aattatgata gattatttaa taaatatcca cacactgaat
    17341 tacaagcttc aggattagaa gttggtcttc cagaaggaca aatgggtaac tcagaggttg
    17401 gtcatttaaa tataggtgct ggtagaataa tataccaaga gttaactaga ataactaaag
    17461 aaataaaaga aggaactttt tttacaaata aagctttagt taaggctatg gatgaagcta
    17521 aagaaaataa tacttcatta cacttaatgg gattattatc aaacggaggg gttcactctc
    17581 acatagatca cttaaaaggt cttttagagt tagctaagaa aaaaggactt caaaaagttt
    17641 acgttcatgc tttcatggat ggtagagacg ttgctccatc atcaggtaaa gaattcatag
    17701 ttgaattaga aaatgctatg aaggaaattg gtgttggtga aatagctact ataagtggta
    17761 gatattacgc aatggacaga gataacagat gggaaagagt agaattagct tataacgcta
    17821 tggttttagg agaaggtgaa aaagcttctt cagctgttga agctatagaa aaatcttacc
    17881 atgataacaa aactgacgag ttcgttttac ctacagtaat tgaagaagat ggacacccag
    17941 tttctagaat taaagatgga gattcagtaa tattcttcaa ctttagacca gatagagcta
    18001 gagaaataac tagagctata gttgatccag aattcaaagg atttgaaaga aagcaattac
    18061 acgtaaactt tgtttgtatg actcaatatg acaaaacttt agagtgtgta gatgttgctt
    18121 acagaccaga aaattacaca aatactttag gagaatatgt tgcgtctaaa ggattaaacc
    18181 aattaagaat tgctgaaact gaaaaatatg ctcacgtaac attcttcttt aacggtggag
    18241 tagaacaacc aaacactaat gaagatagag cattaatagc ttcaccaaag gttgctacat
    18301 acgatcttaa accagaaatg agtgcttatg aagtaactga tgaattaata aagagattag
    18361 atgaagataa atatgacatg ataatattaa acttcgctaa cccagatatg gttggacata
    18421 ctggagtaca agaagcagct gttaaagcta tagaagcagt tgatgagtgc ttaggaaaag
    18481 tagctgataa ggtattagaa aaagaaggta ctttattcat aactgctgac catggtaatg
    18541 cagaagtaat gatagattac tcaactggta aaccaatgac tgctcatact tcagatccag
    18601 ttccattctt atggatatca aaagatgctg aaggaaaatc attaaaagat ggtgggaaat
    18661 tagctgatat agctccaact atgttaactg ttatgggatt agaagttcca tcagaaatga
    18721 ctggaacatg tcttttaaac aaataatata agttagttat atattttaaa tagtattctt
    18781 cttcatgaag gatactattt tttataataa aataagtttt tagcttgtga ttttattact
    18841 catatagggc tttaattttt ttagattaat atttatttta ttaac
//
LOCUS       DAEVGI010000033        18824 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEVGI010000033 DAEVGI010000000
VERSION     DAEVGI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 18824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18824
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.033"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(115..570)
                     /locus_tag="K8M77_001035"
     CDS             complement(115..570)
                     /locus_tag="K8M77_001035"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBI7085546.1"
                     /translation="MVKVESFELDHTKVKAPYVRKAGIKIGPKGDIVSKFDLRFVQPN
                     KELLSDKGMHTLEHFLAGFMREKLDDVIDISPMGCKTGFYLTSFGDIDVKDIIEALEY
                     SLSKVLEQEEIPAANELQCGSAKLHSLELAKSHAKQVLENGISDKFYVE"
     gene            complement(668..1576)
                     /locus_tag="K8M77_001036"
     CDS             complement(668..1576)
                     /locus_tag="K8M77_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBI7085547.1"
                     /translation="MKYYNDIRDLIGNTPILKLNNISTKEGVNIYAKIEGTSPGGSCK
                     DRVGIYMIEKAEKEGKLKPGSTIIEATAGNTGIGIALAAINKGYKIIFIVPDKFSIEK
                     QKIMKALGAEIINTPKEEGMEGAINLANSLLSEIPNSLSLNQFKNEANPLAHYETTGR
                     ELYDGLDGQIDYFVAGAGSGGTISGVLKFLKENISEVKGILADPVGSIIGGGQCGTYK
                     IEGIGNNFIPETMDMSLVDDVIKINDEEAFDAVKLLAKKEGLIVGSSSGAAFAAVLKL
                     AEKIEKGNIVTIFPDRGDRYFSTDLF"
     gene            complement(1563..2717)
                     /locus_tag="K8M77_001037"
     CDS             complement(1563..2717)
                     /locus_tag="K8M77_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HBI7085548.1"
                     /translation="MKKNSLLIHGGISIDEKTGAVNIPIYQTSTYKQRSFGENSGYEY
                     SRTGNPTRESLEKLIADIEYGKRGFAFASGLAAITAVLSLFKSGDKLLITNNLYGGTF
                     RVIDKIFSNFGIGYEIIDDFNNIEEKIDKSVKAIYIETPTNPLLDVIDIKIASDKAKK
                     YNLLTIVDNTFMTPYFQNPLNLGADIVIHSATKYLGGHSDLVAGLVVVKDEELGEKIH
                     FIQNSTGGILQPFDSFLLIRGIKTLSVRMDRHELNANFIAEKLGDLEGIDNVYYPGLK
                     THIGHEIQKKQASGFGGIISFTLNKKYNYEKFFNSLELITFGESLGGVESLACHPASM
                     THAAMPLELRERIGITDRLIRISVGIEDKEDILEDIQQALVKAGDKNEIL"
     gene            complement(2781..3404)
                     /locus_tag="K8M77_001038"
     CDS             complement(2781..3404)
                     /locus_tag="K8M77_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI7085549.1"
                     /translation="MNSVKYFNSIAKEWNKMRVDYFKDELREMAIKSVDISNKVIADL
                     GAGTGFISLGIAKKANLVFSLDSSKNMLKELYSSAKENEINNIYPIKGELENLPLFDD
                     SIDLIFMNMALHHVANPDKAIKEMNRVLKPNGKVVITDVLEHKGEWAREEMFDTWLGF
                     NYDQLINWFEKAGFKNISIKNTGLVARGESSFGEVIEPGIFMSIAKK"
     misc_binding    complement(3547..3785)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4148..4606
                     /gene="mscL"
                     /locus_tag="K8M77_001039"
     CDS             4148..4606
                     /gene="mscL"
                     /locus_tag="K8M77_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBI7085550.1"
                     /translation="MWKEFKEFAMKGNVIDLAIGVIIGGAFGKIVTSLVNDIIMPVIG
                     RLVGKVDFSNLYINLSGQQFNSLQEAQAAGAATINYGLFLNNLINFLIIAFSIFIVIK
                     QINKLKNFTNKNDNIKSEPKEKKCPYCCTKIDIKATRCPHCTSVLEEATN"
     gene            complement(4754..8068)
                     /gene="colA"
                     /locus_tag="K8M77_001040"
     CDS             complement(4754..8068)
                     /gene="colA"
                     /locus_tag="K8M77_001040"
                     /EC_number="3.4.24.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagenase ColA"
                     /protein_id="HBI7085551.1"
                     /translation="MKKNLKRGELTKLKLVERWSATFTLAAFILFNSSFKVFAADKKI
                     ENSNNGQITREINADQISKTELNNEVATDNNRPLGPSIAPSRARNNKIYTFDELNRMN
                     YSDLVELIKTISCENVPDLFNFNDGSYTFFSNRDRVQAIIYGLEDSGRTYTADDDKGI
                     PTLVEFLRAGYYLGFYNKQLSYLNTPQLKNECLPAMKAIQYNSNFRLGTKAQDGVVEA
                     LGRLIGNASADPEVINNCIYVLSDFKDNIDKYGSNYSKGNAVFNLMKGIDYYTNSVIY
                     NTKGYDAKNTEFYNRIDPYMERLESLCTIGDKLNNDNAWFVNNALYYTGRMGKFREDP
                     SISQRALERAMKEYPYLSYQYIEAANDLDLNFGGKNSSGNDIDFNKIKADAREKYLPK
                     TYTFDDGKFVVKAGDKVTEEKIKRLYWASKEVKAQFMRVVQNDKALEEGNPDDILTVV
                     IYNSPEEYKLNRIINGFSTDNGGIYIENIGTFFTYERTPEESIYTLEELFRHEFTHYL
                     QGRYVVPGMWGQGEFYQEGVLTWYEEGTAEFFAGSTRTDGIKPRKSVTQGLAYDRNNR
                     MSLYDVLHAKYGSWDFYNYGFALSNYMYNNNIGMFNKMTNYIKNNDVSGYKDYIASMS
                     SDYGLNDKYQDYIDSLLNNIDNLDVPLVSDEYVNGHEAKDINEITKDIKEVSNIKDLS
                     SNVEKSQFFTTYDMRGTYVGGRSQGEENDWKDMNSKLNDILKELSKKSWNGYKTVTAY
                     FVNHKVDENGNYVYDVVFHGMNTDTNTDVHVNKEPKAVIKSDSSVIVEEEINFDGTES
                     KDEDGEIKAYEWDFGDGEKSNEAKAAHKYNKTGEYEVKLTVTDNNGGINTESKKIKVV
                     EDKPVEVINESEPNNDLEKANQITKSNMLVKGTLSQNDYSDKYYFDVAKKGNVKITLN
                     NLNSVGITWTLYKEGDLNNYVLYATRNDGTELKGEKILEPGRYYLSVYTYDNQSGAYT
                     VNVKGNLKNEVKEVEKDAIKEVENNNDFDKAMKVDSNSKIVGTLSNDDLKDIYSIDIQ
                     NPSDLNIVVENLDNIKMNWLLYSADDLSRYVDYANADGNKLSNTCKLKPGKYYLCVYQ
                     FKNSGTGNYTVNLQNK"
     gene            complement(8610..9065)
                     /locus_tag="K8M77_001041"
     CDS             complement(8610..9065)
                     /locus_tag="K8M77_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine regulator"
                     /protein_id="HBI7085552.1"
                     /translation="MSNLEKSLRHEVILDIIKSKCICKQEELIIELKECGINVTQATL
                     SRDLHEMNIIRKSFENHEHRYIVTKEDKFIKKFKSIFRSSVRSVSMQEYFISVNTLDG
                     MASLIGEFIDRLGDGRIAGTVAKKNHILVLCRSVNATQQIFKELDSLRL"
     gene            complement(9138..10082)
                     /gene="arcC"
                     /locus_tag="K8M77_001042"
     CDS             complement(9138..10082)
                     /gene="arcC"
                     /locus_tag="K8M77_001042"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI7085553.1"
                     /translation="MKIVLALGGNALQKDSKDKSAEGQLETCRQTAVSVADLIEDGHE
                     VSIVHGNGPQVGQILASIELAHQVDNGNPLFPFDVVGAFSEGYIGYHLQNTIREELSK
                     RGIKKSVDTIITQVIVNKNDPGFKNPTKPIGSFYTKEEAEKLEKDKGYTMKEDAGRGY
                     RRVVASPKPVDIVEKEAIKNMVYSGFVVIACGGGGIPVVEDGDRLEGVPAVIDKDFAA
                     EKLAEILDADALLILTAVDRVCVNFNKPDQKALKEINLEEVDKYIEEGQFAPGSMLPK
                     VEACKKFVLSGDKKVAIIASLTNAKAALRGQSGTKIVK"
     gene            complement(10136..11572)
                     /gene="arcD"
                     /locus_tag="K8M77_001043"
     CDS             complement(10136..11572)
                     /gene="arcD"
                     /locus_tag="K8M77_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBI7085554.1"
                     /translation="MAEQHNESKKLGFAQLAALIVGSMIGGGAFNLPSDMARGAGTGA
                     VIIGWIITGIGMIALAFVYQSLANRKPELTGGVYSYAKAGFGEYIGFNSAWGYWLSAW
                     IGNVSYSVLLFGAVGYFLPMFGAGNNVASIIGASVLLWAFALLILNGVSGAALINLIT
                     TIAKLVPIFLFIVISLFMFNFDKFTFQFWGTSDLGGIVTQVKSTMLVTLWCFIGIEGA
                     VVVSGRAKKQSDVGKATVLGLIGTLIIYMLITLMALGVMDRVKLSGLENPSMAYVLEF
                     AVGKWGAVLINLGLIISLFGSLLGWALLAAEIPYVAAKDGVLPKVFAKLNKKGTPSGS
                     LIITNLLVQAFLILTLVASSTYQALYSVASTAILVPYLLSAMYGFKLALTGETYDKDP
                     TGRTRDKIFGGIATVYAIWLCYAAGLKYLLLCFILYAVGIIFFYIGKKQNNKDSVVFV
                     GKEKILAIIVLVIAIIAIVMLFNGAITI"
     gene            complement(11716..12711)
                     /gene="argF"
                     /locus_tag="K8M77_001044"
     CDS             complement(11716..12711)
                     /gene="argF"
                     /locus_tag="K8M77_001044"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBI7085555.1"
                     /translation="MAVNLKGRSFLTLKDFTPAEIRYLLDLSHDLKAKKRAGILGDSL
                     KGKNVVLLFEKTSTRTRCAFECGAAEEGAHVTFLTNSQMGKKESIEDTAKVLGRMYDG
                     IEFRGFKQSTVEELAKHAGVPVWNGLTDADHPTQILADFLTIEEHAHKPLSEIKLVFT
                     GDTRNNMSYALMYGAAKMGMHFVALGPDSLKPDEDILKEMQEYSKETGATIEFSSNVD
                     EAVKGADVIYTDIWVSMGEDESLYPERVKLLTPYKVTREMMNKTGNKNTLFMHCLPSF
                     HDEDTEVCKDMMDRLGLDIREVEDEVFRSKNSVVFDEAENRMHTIKAVMVATAGK"
     gene            complement(12818..14059)
                     /gene="arcA"
                     /locus_tag="K8M77_001045"
     CDS             complement(12818..14059)
                     /gene="arcA"
                     /locus_tag="K8M77_001045"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBI7085556.1"
                     /translation="MRDDRALNVTSEIGRLKTVLLHRPGEEIENLTPDLLDRLLFDDI
                     PYLKVAREEHDAFAQTLREAGVEVLYLEVLAAEAIETSDEVKQEFISEFIDEAGVESE
                     RLKEALIEYFNSFSDNKSMVDKMMAGVRKEELKDYHRESLYDQVNNIYPFVCDPMPNL
                     YFTRDPFATIGHGITLNHMRTDTRNRETIFAKYIFRHHPRFEGKDVPFWFNRDEKTSL
                     EGGDELILSKEVLAVGISQRTDSASVEKLARKLLYYPDTSFKTVLAFKIPVSRAFMHL
                     DTVFTQVDYDKFTVHPGIVGPLEVYAITKDPEKDGQLIAVEECDTLENILKKYLKRDV
                     ELIKCGGGDEIIAAREQWNDGSNTLAIAPGEVVVYSRNYVTNEILEKKGIKLHVIPSS
                     ELSRGRGGPRCMSMPLIREDL"
     gene            complement(14611..16641)
                     /locus_tag="K8M77_001046"
     CDS             complement(14611..16641)
                     /locus_tag="K8M77_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBI7085557.1"
                     /translation="MLHGADYNPEQWIDMPNIWGEDVRLMKLSHTNVVAVGIFSWTML
                     EPEEGKFNFEWLDEIMDLMHKNGNYVILATPSGAKPIWMAHKYPETLRVTPNRVRNLY
                     GERHNHCYTSPIYREKIAIIDRLLAERYKDHPALILWHISNEFEGQCYCPLCEQAFRD
                     FLREKYDNDINKLNKAWWTKFWSHTYASFDEIEAPAPHGEPALHGLNLDWMRFVTHQT
                     LDYYKHERSILKEITPDIPVTTNFHDYISLFRGIDYWKFAPYLDVVSWDNYPYWHGER
                     TDDHEGSRIGFVHDLNRAILNGKPFMMMESSPSSTNWQPVAKLRRPGMHVLSSLQAVA
                     HGSDTVQYFQWRKSRGSSEKFHGAVVDHCGHENTRVFKDVTKVGEILSKLDDVIGTSV
                     EPQVAVIYDWENYWAINDAQGPRIEQKDYFETCQKHYKAFWDMSIPTDVINMDCDFSK
                     YKVVVAPMLYMVRPGVGERLEEFVKNGGTLVTTYWSGIVDENDLCFLGGFPGPLKKVT
                     GIWAEELDALYDEDVNYVSVEEGNSLGMKGEYEARIFCDLIHSEGAEVLATYKTDFYK
                     GMPALTCNNFGEGQAYYIAFRNNDEFLSDFYSSLAKKLTLKRAIEIDLPKGINAQVRM
                     DEKNEFVFFMNFSSEEKTIDIKDLDLTDMVTGEKVTKEMEIEPYGVRIVRRK"
     gene            complement(16837..18324)
                     /locus_tag="K8M77_001047"
     CDS             complement(16837..18324)
                     /locus_tag="K8M77_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI7085558.1"
                     /translation="MSERKRSLSSGALMLMTFTAVFSFGNIIDSSVNIGLATIPSYIF
                     GTVFYFLPFALMIGEFASASSDSESGINSWIKKSLGARWAFLGSWSYFFVNLFFFTSL
                     LPKILIYASYTFVGRNVFDGKTVLISVISIVLFWAVTIISTKGVSWISKITSISGVAR
                     IILGLGFIVLSFGVILFLGKAPAQEFTAETIMPKFNWSYFMVLAWILQAVGGAESIGV
                     YIKDVKGGNKTFIRTMVISTAIVGGLYALGAVSVGLVVPSEVLQGNFSNGLFDAFAIL
                     GANYGVGNIITNIVGFIMMLASLGSLVLWTAAPVKVLFSEIPEGIFGKWIAKTDKKGT
                     PVNALYVQAVIVTVLLLVPALGIGSVDSLLEMLINMTASTSLIPVLFFLVGYIVLRAK
                     KDHMERSFKVGSKNFGIAIGVLLLALFVFVFVISSIPAPQDFAAYFNGTLAEGATNPV
                     FVLLYNVLGLVFFLGFAEICWRKYEKKVGKAVANEWDQEEVSEIA"
BASE COUNT         6000 a         3456 c         2057 g         7311 t
ORIGIN      
        1 taattactta aataattaat tttttactta tagaagattc taacattttt aaaaggctat
       61 atcaaaacaa tttaatagtt ttaatatagc cctttaaatt taaaaatatt atgtttactc
      121 tacataaaat ttatcactta ttccattttc taaaacctgt tttgcatgac tcttagctaa
      181 ctctagtgaa tgtaactttg cacttccaca ttgtaattca tttgctgctg gtatctcttc
      241 ttgctctaaa accttagata gtgaatactc taaagcttct ataatatctt taacatcaat
      301 atctccaaaa ctagttaaat aaaaacctgt tttgcatccc attggtgata tgtctattac
      361 atcatctaat ttttctctca taaagcctgc taaaaaatgt tctaatgtat gcattccctt
      421 atcacttaaa agttctttat taggttgtac aaatctcaaa tcaaactttg aaactatatc
      481 tcctttaggt ccaatcttta ttcctgcttt tcttacatat ggtgccttaa cctttgtatg
      541 atccaattca aaactttcaa ctttaaccac ttaacacatc tccttcgaac tattatataa
      601 atattatctg tttatctttt tacttatttt attaaacaat ttaaaattct attttcttta
      661 atatctatta aaaaagatct gtactaaaat acctatctcc tctatctgga aatattgtta
      721 ctatattgcc cttctctatt ttctcagcta attttaatac tgctgcaaat gccgctcctg
      781 aagaggaacc aactattaat ccttcttttt tagctaataa ttttactgca tcaaaagctt
      841 cctcatcatt tatttttata acatcatcta ctaagctcat atccatagtt tctggaataa
      901 aattgtttcc tattccttct attttataag ttccacattg tccaccacca ataatagaac
      961 ctactggatc agctaatatt ccctttactt ctgaaatatt ttcttttaag aattttaata
     1021 caccagaaat tgttcctccg cttcctgctc cagcaacaaa ataatcaatt tgtccatcta
     1081 atccatcata taattctcta ccagtagtct cgtaatgagc taatggattt gcctcatttt
     1141 taaattgatt taatgataat gaattaggaa tctcacttaa taatgaattt gctaaattta
     1201 tagcaccttc catcccctct tctttaggag tatttattat ttcagcacct aaagctttca
     1261 ttatcttttg cttctctata gaaaatttat caggaactat aaatattatt ttataacctt
     1321 tattaatagc tgccaaagct ataccaatgc ctgtatttcc agcagtagcc tctataatag
     1381 tgcttccagg tttaagttta ccttccttct cagctttttc tatcatatat attccaactc
     1441 tatctttaca actcccccct ggactagtac cttctatttt ggcataaata tttactcctt
     1501 ctttggtact tatattattt agttttaata ttggagtatt tcctattaaa tctcttatat
     1561 cattataata tttcattttt atcaccagcc tttactaaag cttgttgtat atcttctaaa
     1621 atatcctctt tatcttctat accaactgaa attcttatta acctatctgt tattcctata
     1681 cgttctctaa gttctaatgg catagctgca tgagtcatag aagctgggtg acatgccaag
     1741 gactccactc cacctaagct ttccccaaag gttataagtt ctaaactatt aaagaacttc
     1801 tcataattat atttcttatt taaagtgaaa gagattattc caccaaagcc tgaagcttgt
     1861 ttcttttgta tttcatgtcc aatatgagtt ttaagtcctg gataatatac attatctatt
     1921 ccctctaagt cacctaactt ttcagctata aaattagcat ttaattcatg tctatccatt
     1981 cttactgata aagttttgat tcctcttatt agtaagaagc tatcaaaagg ttgaagtatt
     2041 ccaccagtag agttttgtat aaagtgtatt ttttctccaa gctcttcatc tttcacaact
     2101 acaagcccag caactaaatc actgtgcccc ccaagatatt tagttgctga atgaataact
     2161 atatctgccc caagatttaa tggattttga aaatatggag tcataaatgt gttatctact
     2221 attgttaata aattatactt tttagcttta tctgatgcta tttttatatc tatgacatct
     2281 aacagaggat ttgtaggtgt ttctatataa atagctttaa cactcttatc aattttttct
     2341 tcaatattgt taaagtcatc aattatttca taacctatcc caaagttaga gaatatctta
     2401 tcaattactc taaatgttcc accgtaaaga ttattagtaa ttaataattt atctccactt
     2461 ttaaataaac ttaatactgc agtaattgct gcaagacctg atgcaaaggc aaaccctctt
     2521 tttccatatt ctatatctgc aattaacttt tctaatgatt ccctagttgg gtttcctgtt
     2581 cttgaatatt catatccact attttcccca aaagatcttt gtttatatgt ggatgtttga
     2641 tatattggaa tatttactgc cccagttttt tcatctatac ttataccccc atgtattaat
     2701 aaagaattct tcttcataac tacatctcct tttatttatt gctctaaata atatatttaa
     2761 attaattttt tcacatgcta ctatttttta gctattgaca taaaaatacc tggttctatc
     2821 acttccccaa aggacgattc tccacgggca actaatcctg tattttttat acttatattc
     2881 ttaaatcccg ctttttcaaa ccaatttatt aattgatcat agttaaaacc taaccaagta
     2941 tcaaacattt cctctctagc ccactctccc ttgtgttcta acacatcagt tatgaccact
     3001 ttgccattag gttttaatac tctattcatt tccttaatag ccttatcagg attagctaca
     3061 tggtgtaatg ccatattcat aaaaattaaa tctatggaat catcaaagag tggaagattc
     3121 tcaagttctc cttttatggg ataaatatta tttatctcat tttccttggc agaactatat
     3181 aattctttta gcatgttctt agatgaatcc aaagaaaaaa ctaaattagc tttctttgca
     3241 ataccaagac ttataaaacc tgtacctgct cctaaatcag caataacttt attactaata
     3301 tccacagact ttattgccat ttctcttaat tcatccttaa aatagtctac tctcatttta
     3361 ttccattctt tagctatgga attaaaatat tttactgaat tcattttatt agccccctaa
     3421 ttaaaattta aataaactat attttttaaa ttgattaatt ttcaaataat ataataggat
     3481 atatttgcat taaaacaact atataaagca aatttaaaat aaaaaaacct cgtctctata
     3541 aaaatctata gagacgaggt aactcgcggt tccactctaa ttcagatata aaagatatac
     3601 ctgcactcaa aatttagcac taacatgcct aatcactata acgggtgatc ccggagaacc
     3661 ctactaaaaa tttcggattt ctgctccaaa gtgcacttca catttcttct aaatagaaaa
     3721 ctctcaccat cattttcctc tctgtgattt tccaaaatgc tactttactt tttcaatgca
     3781 tttatatcct agtaattgta tctgaaaact agtaattttt attaaatttt aatataattc
     3841 atattaaaaa tcaatacctt tttgaattta tttttaaaaa ttcattaact ctcttaaaat
     3901 aaaatctctt atgcatagat tttttattat tacaaaatat aaaatctata tttaatactt
     3961 tctataatca ctctttaaac taagttaaca taaattatta aaaattgact ttacatacca
     4021 atatctgtta caatttgttt attattttaa tgaaaatttt tatttttaga tattgaatat
     4081 taataaataa tttgtattaa attagttctt ataaacttat attaaaattt aaaaggagga
     4141 aaaaaatatg tggaaagaat ttaaagagtt tgccatgaaa ggaaatgtaa tagacctagc
     4201 tattggtgtt attataggtg gagctttcgg aaaaattgta acttcattag taaatgatat
     4261 tataatgcct gtaattggta gattagttgg aaaagttgac ttctctaact tatatataaa
     4321 cctaagtggg caacaattta actcattaca agaagctcaa gctgctggtg ctgctactat
     4381 aaattacgga ctgtttttaa ataacttaat taatttctta ataatagcat tttctatatt
     4441 tatagtaata aaacaaataa acaagcttaa aaacttcact aacaaaaatg ataatattaa
     4501 atctgaacct aaagaaaaaa aatgtcctta ttgctgtact aaaatagaca taaaagctac
     4561 tagatgtcct cattgtacat ctgttttaga agaagctact aattagtctt cataaaaata
     4621 cataaaactt attttaaaga aactttaagg ctgtatcatc cctcttaaag catagaaatt
     4681 ataaaagtat tgtttataaa ttctatattt gagctttgat acagcctcta tttatactat
     4741 tttaaccatt gttttatttg ttttgtaagt ttactgtgta atttccagta cctgagtttt
     4801 taaattgata aacacataag taatatttac ccggctttaa cttacaagtg ttacttaatt
     4861 tatttccatc tgcattagcg taatccacat atctacttaa atcatcagct gaatataata
     4921 accagttcat ttttatatta tctaagtttt caactactat gtttaagtca cttggatttt
     4981 gtatatctat gctataaata tccttaagat catcattgct taatgttcca actattttgc
     5041 tattactgtc taccttcata gctttatcaa aatcattgtt attttcaact tcttttatag
     5101 catccttttc tacttcttta acttcatttt taaggtttcc ttttacattt actgtgtaag
     5161 ctcctgattg attatcataa gtatatacac ttaaatagta tcttccaggc tctaaaatct
     5221 tttcaccctt taattctgtt ccgtcatttc tagttgcata taaaacataa ttgtttaggt
     5281 ctccctcttt ataaagtgtc caagttattc ctactgaatt taaattatta agagttattt
     5341 taacatttcc ttttttagct acatcaaaat aatatttatc tgaataatca ttttgtgata
     5401 aagtaccctt aactaacata ttagatttag ttatttggtt agctttttcc aaatcattgt
     5461 taggctcact ttcatttata acttcaacag gtttatcttc tactactttt atctttttac
     5521 tttcagtatt tattccacca ttattatctg taactgttaa ttttacttca tattctccag
     5581 ttttattata tttatgagca gctttagcct cattagattt ttctccatct ccaaagtccc
     5641 attcataagc tttaatttca ccatcttcat cttttgactc tgttccatca aagtttattt
     5701 cttcttcaac tattactgaa gaatcagatt ttataacagc cttaggctct ttatttacat
     5761 gaacatcagt atttgtatct gtattcattc catggaatac aacatcataa acatagttac
     5821 cattttcatc tactttatgg tttacaaagt atgcagtaac agttttatac ccattccagc
     5881 tctttttaga taattctttt aatatatcat ttaacttaga attcatatct ttccagtcat
     5941 tttcttcccc ttggcttctt ccccctacat atgttcctct catatcgtaa gtagtaaaga
     6001 attgagactt ttcaacatta ctagaaagat cttttatatt tgaaacttct tttatatcct
     6061 tagttatttc atttatgtcc ttagcttcat gtccatttac atattcatct gaaactaaag
     6121 gaacatctaa gttgtcaata ttatttaata aagaatctat atagtcttga tatttatcat
     6181 ttaatccgta atcactactc attgatgcaa tataatcttt ataaccagat acatcattat
     6241 tctttatgta atttgtcatc ttattaaaca ttccaatgtt attgttgtac atgtagtttg
     6301 ataaagcaaa tccataatta tagaaatccc atgagccata tttagcatgt aatacatcat
     6361 ataaagacat cctattattt ctatcgtaag ctaatccttg tgtaactgat tttcttggtt
     6421 ttattccatc agttctagtt gaacctgcaa agaactctgc tgttccttct tcataccaag
     6481 ttaaaactcc ctcttgatag aattctcctt gtccccacat tccaggaact acatatctac
     6541 cttgaagata gtgagtaaat tcatgacgga ataattcttc taatgtatat atactttcct
     6601 ctggtgttct ttcataagta aagaaagttc ctatgttttc aatatatata ccaccattat
     6661 cagtactaaa tccatttatt atacgattta acttatactc ttctggtgag ttataaataa
     6721 caacagttaa aatatcatct ggatttccct cttctaaagc cttatcattt tgaactactc
     6781 tcataaattg agccttaact tcctttgaag cccagtataa tctttttatc ttttcttctg
     6841 ttactttatc accagctttt actacaaatt tgccatcatc aaaagtatat gtttttggaa
     6901 gatatttttc ccttgcatct gcttttatct tattaaaatc tatatcattt cctgatgaat
     6961 ttttgccacc aaaatttaaa tctaaatcat tggcagcttc aatatattga tatgataaat
     7021 aaggatactc cttcatagct ctttctaaag ctctttgaga tattgatggg tcttctctaa
     7081 acttacccat tctacctgtg taatataagg cattatttac aaaccaagca ttatcattat
     7141 ttaacttatc acctattgta cataaacttt ctaatctttc catatatgga tctattctat
     7201 tatagaactc agtgttttta gcatcatatc ccttagtatt gtatattact gaattagtat
     7261 aataatcaat acctttcata aggttgaata ctgcatttcc cttgctatag ttcgaaccat
     7321 acttatctat attatcttta aaatcactta agacatatat gcaattatta ataacttctg
     7381 gatctgctga agcattacct ataagtcttc ctaaagcctc aacaactcca tcttgcgcct
     7441 ttgttcctaa tctaaaatta ctattatatt gaatcgcttt catagctggt aaacactcat
     7501 tttttaactg tggtgtattt aggtatgata attgtttatt gtaaaatcct aaataatatc
     7561 cagctcttaa aaactcaact aaagttggaa ttcccttatc atcatctgct gtataagttc
     7621 ttccactatc ctctagacca tatattatag cttgtacacg atctctatta ctaaagaaag
     7681 tatatgaacc atcattaaaa ttaaataagt ctggtacgtt ttcacaactt attgttttta
     7741 ttaattcaac tagatcacta taattcattc tgttaagttc atcgaatgta tagatcttgt
     7801 tgtttcttgc tcttgatgga gcaatactag gtcctaatgg tctattattg tctgtagcta
     7861 cctcattatt taattctgtt ttagaaatct gatcagcatt aatctctcta gtaatctgtc
     7921 cattattact attctctatt tttttatcag ctgcaaatac tttaaacgag ctattaaata
     7981 aaataaatgc tgctaaagta aaggtagctg accatctttc aactaacttt agtttcgtta
     8041 gctctcccct ttttaagttt ttcttcatgt ttaaatgcct cctctatctt aaacaatatt
     8101 tatataaata aaactttgct tatttaagct gaagttttct tatatacata atttaaaata
     8161 accttacaca tttaaaaatc attcaccatt tgttattaat ttagtaactt tttaattata
     8221 acataaaatt tgtaattttt tatatttcat ttctaaataa tcctattatt ctttattttt
     8281 tatagttatt tttttaatac attaataact caaattaaat gtattattat tccagttaat
     8341 cgcacaaaaa aatattacgg tgattcaatg tactttattg gcaactcagt aaattacact
     8401 cttcaccgta atattaaaag gtatttttta ggtaattgtt taagactttt tataaactaa
     8461 aatatctttg tattccttac tactgttaaa gtgtaaaaaa tataaaatat tttagtctat
     8521 ggattttttg tataatgatt aattatacaa tgcaacattt aagctattag tatttattct
     8581 taactctttg tagagttaat tgcttttaat tataatctta aggaatctaa ctctttgaat
     8641 atctgctgag tagcattaac acttctgcac aaaactagaa tatgattttt tttagctact
     8701 gttccagcta ttcttccatc accaagccta tctataaact ctccaatcaa actagccata
     8761 ccatctaatg tattaactga tatgaaatat tcttgcatag atacgcttct tacagaactc
     8821 ctgaatatac ttttaaactt tttaataaac ttatcttcct ttgtaactat atatctatgc
     8881 tcatgatttt caaaactttt tctaattata ttcatctcat gaagatctct tgacaaagtg
     8941 gcttgagtta catttattcc gcattctttt aactcaatta ttaattcttc ttgtttacag
     9001 atacatttgc tctttattat atctaatatt acttcatgtc ttaaagattt ctctaaatta
     9061 ctcataatat ctcctctgct ccctttaacc tcgtatgtat gctatataaa caatgaaatt
     9121 aatatatata ggcaatttta tttaactatt tttgttcctg attgtcctct taaagcagct
     9181 ttagcattag ttaaagatgc aattatagct acctttttgt ctccgcttag tacaaacttc
     9241 ttacaagctt caacctttgg aagcatactt cctggtgcaa attgaccttc ttctatatat
     9301 ttatctactt cttctaaatt aatttctttt aaagcctttt gatctggctt gttgaagttt
     9361 acacaaactc tatcaactgc tgttaagatt aataatgcat ctgcatctaa tatttcagct
     9421 aatttttcag cagcaaagtc cttatctata accgctggaa ctccttctaa tctatctcca
     9481 tcttctacta cagggattcc accgcctccg caagcgatta caacaaagcc actatatacc
     9541 atatttttta tagcttcttt ttctactata tctactggtt ttggtgaagc tactactctt
     9601 ctatatcctc taccagcatc ttctttcata gtatatcctt tatccttttc tagcttttct
     9661 gcctcttctt ttgtatagaa agaacctatt ggtttagttg gatttttaaa accaggatca
     9721 tttttgttaa cgattacttg agttataatt gtatctactg atttttttat tcccctcttt
     9781 gataattctt ctctaattgt gttttgaaga tgataaccaa tataaccttc tgaaaaagca
     9841 ccaactacat caaatgggaa taatggattt ccattatcaa cttgatgagc taattcaata
     9901 cttgctaata tttgtccaac ttgtgggcca tttccatgaa ctattgatac ttcatgtcca
     9961 tcttctatta aatcagctac tgaaactgct gtttgtctac atgtctctaa ttgaccttct
    10021 gcacttttat cctttgaatc cttttgtaat gcgtttccgc ctaatgctaa aacaattttc
    10081 ataagtcttt tcctccttca tattaatcac tttaacttta attttatttt tatatctaaa
    10141 tggttattgc tccgttaaat aacattacta ttgctataat tgctataact aaaacaatta
    10201 ttgctagtat tttttctttt cctacaaata caacactatc tttattattc tgttttttac
    10261 caatgtagaa gaatataatt cctactgcat ataatatgaa acataataat aaatatttaa
    10321 gtcctgctgc atagcatagc catatagcgt aaacggttgc tattccacca aaaattttat
    10381 ctctagttct tcctgtagga tctttatcgt aagtttcacc tgttagtgct aacttaaatc
    10441 catacatagc acttaaaaga tatggtacta atatagctgt actagctact gaatatagag
    10501 cttgataagt actgcttgct actaaagtta atattaaaaa tgcttgtact aataaattag
    10561 ttattattaa tgaaccactt ggtgttccct ttttatttaa tttagcaaag actttaggta
    10621 ataccccatc ctttgctgct acataaggaa tttctgctgc aagtaatgcc catccaagaa
    10681 gtgatccaaa taatgaaatt atcaacccta aatttataag aactgctccc catttaccaa
    10741 ctgcaaattc aagaacatat gccattgatg ggttttcaag acctgaaagt ttaactctgt
    10801 ccataactcc aagtgccatt aatgttatta acatatatat tattaatgtt cctataagac
    10861 caagtactgt tgccttacct acatcgcttt gtttcttagc tcttcctgaa acaactacag
    10921 ctccctcaat tcctatgaag caccataatg ttactagcat agtactcttt acctgtgtta
    10981 caattccacc taaatcactt gtaccccaaa attggaaagt aaatttatca aaattaaaca
    11041 taaataatga gataactata aataagaata ttggaactaa cttagctata gtggttataa
    11101 gatttataag tgctgctcca cttaccccat ttaatattag taatgcaaat gcccaaagta
    11161 atactgatgc gccaataata gatgctacgt tatttcctgc accaaacata ggtagaaaat
    11221 aacctactgc tccaaataga agtactgaat aagatacatt tcctatccat gcacttaacc
    11281 aatatcccca agctgaattg aatcctatat attctccaaa ccctgctttc gcataactat
    11341 aaacaccacc tgttaattca ggctttctgt tagctaatga ttggtaaaca aaagctaaag
    11401 ctatcatacc aatacctgtt ataatccatc ctattataac tgctccagta ccagctcccc
    11461 ttgccatatc tgatggaaga ttgaaagctc caccaccaat catagaacca actattaagg
    11521 ctgctaattg agcaaaccct aatttttttg attcattatg ttgctctgcc atataacttc
    11581 acccactttc atattattaa aaacagggca gcatttataa tgcaatgaaa ggaaaattca
    11641 tttattaaaa ttaaatactt taaattctaa ttattcctta gatgatatta taaatacagc
    11701 ccttaagaaa atttattatt ttccagcagt tgctaccatt actgctttta ttgtatgcat
    11761 tctgttttca gcttcatcaa atacaactga atttttgctt ctaaatactt catcttcaac
    11821 ttctcttata tcaagaccta atctatccat catatcttta catacttctg tatcttcatc
    11881 atggaatgat ggtaggcaat gcatgaataa agtattctta tttcctgttt tattcatcat
    11941 ttctctagta actttatatg gagttaataa tttaactctt tctggatata agctttcatc
    12001 ttcacccatt gatacccaga tatcagtgta gataacatct gcacccttaa ctgcttcatc
    12061 aacatttgaa ctaaattcta tagttgcacc agtttctttt gagtattctt gcatttcttt
    12121 aagaatatct tcatctggtt ttaagctatc tggtccaaga gctacaaagt gcattcccat
    12181 tttagctgca ccatacatta acgcataact catgttgttt cttgtatcgc cagtaaatac
    12241 taatttaatt tcacttaatg gtttatgtgc atgttcttct attgttaaga aatctgctaa
    12301 tatttgagtt ggatggtcag cgtcagttaa tccattccat actggaacac ctgcatgttt
    12361 agctaattct tcaacagttg attgtttgaa tcctctaaat tcaataccat catacattct
    12421 tccaagaacc ttagcagtat cttctattga ttctttttta cccatttgtg aattagttaa
    12481 gaatgttaca tgagcacctt cttcagcagc tccacattca aaagcacatc ttgttctagt
    12541 tgaagttttt tcaaatagta aaactacatt tttacctttt aagctatctc ctaatattcc
    12601 agctctcttt ttagccttta aatcatgaga aagatctaat aaatatctta tttctgctgg
    12661 tgtaaagtct tttaatgtta aaaagcttct tccttttaag ttaactgcca ttttgaattc
    12721 ctcctaatat taaatattct tatatacatt tttttgtaag tatgagaata ttaatttttt
    12781 taaatttcta atagtaaatt actagttaaa tgttatctta taaatcttct cttattagag
    12841 gcattgacat acatctagga ccccctctac ctcttgataa ttcagatgaa ggaataacat
    12901 gtaacttaat tcctttcttc tctaatattt cattagttac atagtttctt gaataaacta
    12961 caacctctcc tggagcaata gcaagtgtat ttgaaccatc attccattgt tctctagcag
    13021 ctattatttc atctcctcca ccacatttta ttaactcaac atctctcttt aagtatttct
    13081 ttaatatatt ttctaaagta tcacattctt caactgcaat tagttgtcca tctttttctg
    13141 gatctttagt tatagcataa acttctaaag gtcctactat gccaggatga actgtaaatt
    13201 tatcataatc aacttgagta aatactgtat ctaaatgcat aaatgctctt gatactggta
    13261 ttttaaatgc taatacagtt ttaaagcttg tatctggata gtaaagtaac tttctagcta
    13321 atttttcaac tgatgctgaa tcagttcttt gtgatatacc aactgctaaa acttcttttg
    13381 aaagtattaa ttcatctcca ccttcaagtg aagtcttttc atctctattg aaccagaatg
    13441 gaacatcctt cccttcaaat cttggatgat gtctaaatat atatttagca aatattgttt
    13501 ctctatttct tgtatctgtt ctcatgtgat ttaatgtaat accatgtcca attgttgcaa
    13561 atggatctct tgtaaaataa agatttggca ttggatcaca tacaaatgga tatatattat
    13621 ttacttggtc atataatgat tctctgtggt aatccttaag ttcttctttt cttacaccag
    13681 ccatcatctt atccaccatt gatttattat cactaaatga gttgaagtat tctattaatg
    13741 cttcttttaa tctctcgctt tcaactccag cctcatcaat aaattcactt ataaattctt
    13801 gttttacctc atcactagtt tctatcgcct cagcagcaag aacttctaaa taaagcactt
    13861 ctactcctgc ttctcttaaa gtttgtgcaa aagcgtcatg ttcttctctt gcaactttta
    13921 agtatggaat gtcatcaaat agtagtctat ctaatagatc tggtgttaaa ttttcaattt
    13981 cttctccagg tctatgtaat agaactgttt ttaatcttcc tatttcagaa gtaacattta
    14041 atgctctgtc atctctcata atataccctt cctccctctg tattttaaga ttttgtaggt
    14101 tgttttcctt cccacaccta tgttatacac cattaaaaaa acgattacaa gagcacctca
    14161 tggaaattat tgcatatatt ttttcatttt tattcaaaaa gatactatct agtaaatagg
    14221 tttattaaac aatttctgta aaatacatgt attacaatta aaattaataa ttgattataa
    14281 tgaatatata atgtataatt accgaataat attttttaaa aactttaata ttaaaccctt
    14341 ttcatgttaa atttattcat aaatttaatg taaatatttc aaattcttct tattttttct
    14401 ataaatgctt atattaataa ataataaaag ttataattct ttagtttaat ctcttctaaa
    14461 aaaaactatt gataaaaata aagacaagta agttttcacc tacctgtctc atataaaaaa
    14521 ttcaatttta actaaaagta ttcatcatta cttttttctt tttaatctat taaactaact
    14581 ataaatctta tttatacgtt aatatctatt ttattttctt ctaactattc taactccata
    14641 aggttctatt tccatttcct tagttacctt ttcaccagtt accatatctg ttaaatcaag
    14701 atctttaata tctattgttt tttcctctga tgagaagttc ataaagaata caaactcatt
    14761 cttttcatcc atacgaactt gagcattaat tccctttggt aagtcaattt ctatagctct
    14821 ctttaaagtt aacttcttag ctaagcttga gtagaagtct gataagaact catcattatt
    14881 tctaaatgct atataataag cttgcccctc tccaaagttg ttacaagtta aagctggcat
    14941 tcctttgtag aagtctgttt tgtatgttgc aagaacttca gctccttctg agtgaattaa
    15001 gtcacagaat attctagcct catattcacc tttcattcct aagctattgc cttcttcaac
    15061 agaaacatag tttacatctt catcataaag tgcatctaat tcttctgccc agattcctgt
    15121 aactttcttt aatgggccag ggaatcctcc taagaagcaa aggtcattct catcaacaat
    15181 accactccag taagttgtta ctaaagttcc tccattttta acgaattcct ctaatctttc
    15241 tccaacacct ggtcttacca tataaagcat tggtgctaca accaccttgt attttgagaa
    15301 atcacaatcc atgtttataa catctgttgg aattgacata tcccagaaag ctttatagtg
    15361 tttttgacaa gtttcaaagt aatccttttg ctctattcta ggaccttgag catcatttat
    15421 tgcccagtaa ttttcccaat cgtaaataac agctacttgt ggctctacag aagttcctat
    15481 tacatcatca agttttgata gaatttctcc aaccttagtt acatctttaa acactcttgt
    15541 attttcatgt ccacagtggt caacaacagc tccatggaat ttttctgatg atcctctact
    15601 ctttctccat tggaaatatt gaactgtatc tgaaccatga gctacagctt gaagagaaga
    15661 taaaacatgc attcctggac gtctaagttt tgcaactggt tgccagtttg ttgaacttgg
    15721 agaactttcc atcatcataa atggcttacc atttaaaatt gctctattta agtcatgaac
    15781 aaatccaatt ctacttcctt cgtggtcatc tgttctttct ccatgccagt aaggatagtt
    15841 atcccatgat acaacatcta agtaaggagc aaatttccag taatctattc ctctaaataa
    15901 tgaaatatag tcatggaagt tagtagttac aggaatatct ggtgtgattt cctttaatat
    15961 gcttctttca tgcttgtagt aatctaaagt ttggtgagta acaaatctca tccaatctaa
    16021 gtttaatcca tgaagagctg gttctccatg aggagctggt gcttctattt catcaaagga
    16081 agcatatgtg tgactccaga acttagtcca ccaagcttta tttaatttat ttatgtcatt
    16141 atcatatttc tctcttaaga aatctctaaa agcttgttca cataaaggac agtaacattg
    16201 accttcaaac tcatttgaaa tgtgccataa gattaaagct gggtgatctt tatatctttc
    16261 agctaataat ctgtctatta tagcaatttt ctctctatat ataggagatg tataacagtg
    16321 attatgacgc tctccataaa ggtttcttac tctgtttgga gtaactctta aagtttctgg
    16381 atacttatga gccatccata ttggcttagc tccacttggt gtagcaagta ttacatagtt
    16441 tccattcttg tgcattaaat ccatgatttc atctaaccat tcaaagttaa acttaccttc
    16501 ctctggctct aacattgtcc atgaaaatat gcctacagct acaacatttg tatgagaaag
    16561 cttcattaat ctaacatctt ctccccatat gttaggcata tcaatccatt gttctgggtt
    16621 gtagtctgct ccatgaagca tttttgttac tttagaactt attggtccat aatttttcaa
    16681 aataatccct ccatcttacc aagttaatca tttttttctt attaatctat aaagtaaatt
    16741 gtgtatttct aaataaattt atagttatag gaataagcta tatttttata gcttattcct
    16801 taattgatat cttttataat tttgtcatta ctttaattaa gctatttctg aaacctcttc
    16861 ttgatcccat tcattagcta cagcttttcc aactttcttt tcatattttc tccagcagat
    16921 ttcagcaaaa cctaagaaga atactaatcc taatacattg tataaaagta caaatacagg
    16981 atttgttgct ccttctgcta atgttccatt aaagtaagct gcaaagtctt gtggagctgg
    17041 aattgaagat attacaaata cgaatacgaa taaagcaagt aataatactc caattgctat
    17101 tccaaagttt ttagatccaa ctttaaatga tctttccata tggtctttct tagctcttaa
    17161 tacaatgtat ccaactaaga agaataatac tggaattaat gaagttgaag cagtcatgtt
    17221 tattaacatt tcaagtaagc tatcaactga acctattcct aaagctggta ctaatagtaa
    17281 tactgttact attacagctt gtacatataa agcatttaca ggagttcctt ttttatctgt
    17341 tttagcaatc catttaccaa agattccttc aggaatttca gagaataata ctttaacagg
    17401 tgctgcagtc cataaaacta atgaacctaa agaggcaagc atcattatga atccaactat
    17461 gttagttatt atgttaccta ctccatagtt tgcacctaat attgcaaagg catcaaataa
    17521 accatttgag aaattacctt gtaatacttc tgaaggtaca actaaaccta ctgaaacagc
    17581 tccaagtgca tataaaccac caactatagc agttgaaatt accattgttc ttatgaatgt
    17641 tttatttcca ccctttacgt ctttaatata tacacctata ctttcagcac cacctactgc
    17701 ttgtaaaatc caagctaaaa ccatgaaata tgaccagtta aacttaggca taatagtttc
    17761 tgctgtgaat tcttgagctg gagcttttcc taagaataaa ataactccga aagataatac
    17821 tatgaatcct aaccctaaaa ttattctagc tacaccagat atacttgtta tttttgaaat
    17881 ccaagataca cccttagtag aaattatagt tactgcccag aataatacta ttgatataac
    17941 agaaattaat actgtttttc catcaaatac atttctacca acaaatgtat aagatgcata
    18001 aattaatatt ttaggtaata gtgaagtaaa gaagaacaag ttaacaaaga aataagacca
    18061 tgatcctaag aaagcccatc ttgctcctaa agatttcttt atccaactat ttatacctga
    18121 ttcagaatct gaactagctg aagcaaactc accaatcatc agagcaaatg gtaaaaagta
    18181 aaatactgta ccaaatatgt atgatggtat tgtagctagt ccaatattta cactactatc
    18241 aattatgttt ccgaatgaaa ataccgctgt aaatgtcata agcattaaag ctcctgaact
    18301 aagcgacctt tttctctcac tcatcttaaa attccccctc atatctcata ttttaaatat
    18361 aaatcccctt ttaggaaacg tttactaaga ttataatatc attttttgtg gaaatattaa
    18421 tgtacaaaat tttaatattt agtaaaaaaa ttatattttt actaaattgt tttactaaat
    18481 catgcttaat tgaaatattt ttaatactaa gtattatgca taatatttat aaaaatctct
    18541 ctttattgat atattgtata aaaatattgt tattttttaa tattttatca ttattttcta
    18601 attaaattaa cttactaaca atgaaatata atcttttctt ttatttcttt aagtttttta
    18661 gttaaagttt actccttatt atttattaga gattaacctt gattttctat gaattatatt
    18721 gaaatttatt tattattacc cttaataatt tattataatt tatatttttt gattttatta
    18781 gaaaataatt ttaaagaaac tttataatta attgtttttg aata
//
LOCUS       DAEVGI010000034        18492 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEVGI010000034 DAEVGI010000000
VERSION     DAEVGI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 18492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18492
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.034"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     misc_binding    184..354
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            515..3154
                     /gene="alaS"
                     /locus_tag="K8M77_001048"
     CDS             515..3154
                     /gene="alaS"
                     /locus_tag="K8M77_001048"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBI7085559.1"
                     /translation="MKFMGANELREKYLSFFESKDHLRLQSFPLVPKNDKSLLLINAG
                     MAPLKPYFTGLEEPPKRRITTCQKCIRTGDIENVGKTSRHGTFFEMLGNFSFGDYFKS
                     EIIPWAWEFITETLGIPKDKLYVTIYLNDDEAYDIWTSKTDVDPSRIFRLGKDDNFWE
                     IGVGPCGPCTEIHFDRGEGKVETVEEFLEASDADRIVEFWNLVFTQFDKDEEGNYNEL
                     AQKNIDTGMGLERIATIMQGVDNIFEIDTVKNILNKACELTNAKYGEDKDKDVSLRII
                     TDHGKSVTFLICDGVQPSNEGRGYVLRRLLRRAARHGRLLGVKGIFLNEMVDAVVENY
                     GEAYPELKEKADYIKKIIKLEEERFNETIDSGMDILMSYISEMEEKNEKVLSGAKAFK
                     LYDTYGFPLELTQEILEEKGLELDIENFNKEMKEQRERARNARGESSYMGSEESPVNK
                     VDASIVTEFDGYVNLELNSKVIVLGNNEEFKAELKEGEEGFLLTDKTPFYAEMGGQVG
                     DRGNITSETGMAIVTDCKKNVGGKFVHYIKVIEGSLKEGQEVKLSVDASRRSNICKNH
                     TATHILHEALKEILGDHVNQSGSYVDEERLRFDFTHFAALTEEELEKVELLVNEKIMT
                     VSVVDTKEMSLDEARNSGATCLFDEKYAEKVRVVSVGDFSKELCGGTHVANSGEIGLF
                     KIVSESGVAAGIRRIEAVTGISALKFMELKNNMLKEAASMLKCNEKDIAKRIAAQAHE
                     LKEKDKEIAELKAKLVQGAEDDILKDKVEINGVELVTAELKDVDGNSLRDLADKVRNK
                     LNNGIVVLASDNGGKVNLVAMATKNSLANGVHCGKVIKEVAAVVGGGGGGRPDMAQAG
                     GKNPENIAKALEKAKEVVELLVK"
     gene            3298..3555
                     /locus_tag="K8M77_001049"
     CDS             3298..3555
                     /locus_tag="K8M77_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBI7085560.1"
                     /translation="MSNNFDHTMQFDFSKNKEDLTKSILTDVYNSLKEKGYNPVNQLV
                     GYLISGDPTYITNYNGARALVRKLERDEILEEVIKSYLEIK"
     gene            3603..4016
                     /gene="ruvX"
                     /locus_tag="K8M77_001050"
     CDS             3603..4016
                     /gene="ruvX"
                     /locus_tag="K8M77_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBI7085561.1"
                     /translation="MRILGLDIGSKTIGVAVSDPLGWTAQGVTTIKRDCYTKDVEAVM
                     KICKEYGVETIVAGMPKNMNGTIGPSGEMVKNLCEQIEKSFDGKIEFWDERLTTVAAH
                     RAMLEADLSRAKRKKIVDKIAATYILQGYLDRISK"
     gene            4032..4286
                     /locus_tag="K8M77_001051"
     CDS             4032..4286
                     /locus_tag="K8M77_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBI7085562.1"
                     /translation="MNNDLQPIVLVDEEGIETTFNVVTKLDIEEKEYFLLSPEGEEDV
                     VIAMQVVQDEDGEETLAPVENDFEIEMIEEAYATLFAEEE"
     gene            4307..4759
                     /locus_tag="K8M77_001052"
     CDS             4307..4759
                     /locus_tag="K8M77_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBI7085563.1"
                     /translation="MANTASVDFDALKEELKKKGYKLTPQRRAIVDTIIQNEGKHLTA
                     EEIYDEVKKTCPEIGLATVYRTIILLEEMGVIYKLDLEDGCSRYELAHDDEEHRHHHL
                     VCNKCGKVFEVEADLLDELEEEIETKYGFEILDHTVKFFGICKDCKNE"
     gene            4830..6497
                     /locus_tag="K8M77_001053"
     CDS             4830..6497
                     /locus_tag="K8M77_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBI7085564.1"
                     /translation="MKREKLKVKIIPLGGLNEIGKNLTVIEFKDDIIVVDCGLKFPDE
                     DMFGIDIVIPDVSYLVKNAEKVRGIFLTHGHEDHIGALPYVLKNLNVPVYGTKLTLGI
                     VETKLKEHGLLSTTELIRVKPRDIIKLKSSSVEFVKTNHSIADSVAIAVHTPLGVVLH
                     TGDFKVDYTPTDGEVMDFARFAELGRKGVLAMMADSTNVERPGYTMSERAVGENLKKI
                     FIGAKGRIIIATFASNIHRIQQIVEAAEMTGRKIAVSGRSMENIVQVAIELGYLTIDK
                     DSFVSIDSINKYPNEQVTIITTGSQGEPMSALARMASSEHKKVNIIEGDTVILSATPI
                     PGNEKLVSKVINQLFKKGAEVVYGKLADVHVSGHACQEELKLMQALVKPKFFIPVHGE
                     YRHLKQHAELAVDLGLSEKNFMIAENGDVIEITRDSIKKNGSVTSGQIFVDGLGVGDV
                     GNIVLRDRKHLSQDGILTVVVTISKEAASVVAGPDIISRGFVYVRESEDLMDEAKEIV
                     KCILRDCEKKGICDWATIKSNIRDGLRSFLYEKTKRKPMILPIIMEI"
     gene            6653..8485
                     /gene="typA"
                     /locus_tag="K8M77_001054"
     CDS             6653..8485
                     /gene="typA"
                     /locus_tag="K8M77_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBI7085565.1"
                     /translation="MSNEFTRNDIRNIAIIAHVDHGKTTLVDALLRQSNVFRTNEKVE
                     ERVMDSNDLEKERGITILSKNTAVHYDGVKINIIDTPGHADFGGEVERVLKMVESVLL
                     VVDSYEGAMPQTKFVLRKALELGLKPIVVINKIDKPDARPEEVIDEIFELFLELGADD
                     EQLDFPIVYASARDGVATINIDEPKDNMKDLFDTIIEKVESPKGSIEDSLQMLVTTLD
                     SSEYVGKIAIGKITRGVAKKNQQAAVVRQDGTVNKFKISSLYTHDGLKRIEVEEAQLG
                     DIVAISGISDVNIGETITDAQNPEGLPFVKIDEPTLNMNFMVNDSPFAGREGDFVTSR
                     HLRDRLLKELETNVSLKVKEITPDCFEVSGRGELHLSILIETMRREGYEFQVSKPNVI
                     TKIDENGVKVEPIEHLTIDVPEEFMGPVMEKLGPRKAEMVNMTSAVNGYSRLEFKIPA
                     RGLIGFRNEFMTDTKGNGIMNHVFDGFEKYKGEIPGRSRGSIVVFESGEAVTYGLFNA
                     QERGTLFIEPGTEVYAGMVCGECSRADDIDVNICKKKQLTNTRSSGADDALKLTPVRQ
                     MSLEQCLEFINNDELVEVTPVNVRMRKRILDSAERKRAINRSKK"
     gene            8627..9655
                     /gene="mltG"
                     /locus_tag="K8M77_001055"
     CDS             8627..9655
                     /gene="mltG"
                     /locus_tag="K8M77_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBI7085566.1"
                     /translation="MKKLKNSKSMVLISIFIILLVINLAVFVVKYNSIKRSPLQSNKA
                     DITFKVKDGESLNGLFERLNNENVLRSSFFSKIYIKFNNVEETIKPGTYTVNSDISFK
                     DFISVLTDGKVSDYKLTFPEGYTVEDIAKKLDESKVCTKDEFLKVEKEYPLPSYIKPN
                     NERKYELEGFLFPDTYAIPKGTTPKQIIEMMLNRFEGVISEIQSELGITISKEEYEKY
                     VIVASMVEKEARDDSERAEIASVIYNRLQKGMPLQIDATVLYALGEHKDTVLYKDLKV
                     DSPYNTYKIKGLPVGPICNPGKPSLLAAIKPAKTDYIYYLLNPSNNKHYFTNNYEDFI
                     AKKKEFGY"
     gene            9671..10315
                     /locus_tag="K8M77_001056"
     CDS             9671..10315
                     /locus_tag="K8M77_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBI7085567.1"
                     /translation="MSGVTFEYMEKYLRDLIPDRNDELKELEDFAKENKVPIIQKEGA
                     KFLEFMVSMNKPKKILELGTAIGYSSILMNKACGGECHITTIERDDNMINYAKENIKK
                     FGLEEKINILQGDCLEVLESLQDEFDMIFMDAGKGHYNHFLPECLRLLKKDGIIIADN
                     VLFRGMVASDDLVKRRKITIVKRMRKYLDMVSKDENLITTVIPMGDGIALTKRR"
     gene            10321..11550
                     /locus_tag="K8M77_001057"
     CDS             10321..11550
                     /locus_tag="K8M77_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI7085568.1"
                     /translation="MRKPEILAPAGSLEKLKTAIDFGADAVYIGGSKLNLRAFADNFT
                     NEQIAEGVKYAHDRGRKVYVTMNVFPHNADLEGLEEYIVGLNDLNVDAIIVSDPSIIM
                     TAKEVAPDLEIHLSTQANNVNWKSAKFWHSLGVKRIVLARELSFKEIEKIHENLPEDC
                     DLEAFVHGSMCMAYSGRCLISNYMTGRDSNRGACSQACRYKYYLMEEKRPGEYFQVIE
                     DDKGTYIMNSKDLCMIEYIPELVKSGIYSFKIEGRMKSPYYVAAIVKAYREALDKYWD
                     DPEGYEFDQKLMDNLLKVSHRRYHTGFYFGKSGEQVYESSSYIRDYDIVGVVRDYNEE
                     TKVATIEQRNRLFEGDKVEVLTPVGDYYEIQMNDMKDEKDEKIDVANKAQMIFKVKID
                     KPVKVNDMLIKCKEANS"
     gene            11550..12176
                     /gene="udk"
                     /locus_tag="K8M77_001058"
     CDS             11550..12176
                     /gene="udk"
                     /locus_tag="K8M77_001058"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBI7085569.1"
                     /translation="MKRPIFIGITGGTGSGKSTIAKEIYRQFGEECIAMIEQDSYYKD
                     QSHLSMDDRVKTNYDHPNAFDNNLLVSHLESLLNGHCIQKPSYDFSIHNRIEDTTKVE
                     PKEIVIVEGILILEDPRIRELLDIKIYVDTDADVRIIRRMVRDINERGRTIESVINQY
                     LNVVKPMHNQFTEPTKKFADIIIPEGGHNKVAIDIIVAKIKEVLGKYE"
     gene            12288..13955
                     /locus_tag="K8M77_001059"
     CDS             12288..13955
                     /locus_tag="K8M77_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBI7085570.1"
                     /translation="MFNRKRKIEIKNRLISLVSLLLLALLLIDMRIYYVQKKYSTKET
                     GNFKGNHKQYENISDLNYSLLDINGESIFQYDTKYKVVIDSMAFRLNNLNQNLENIMA
                     FNYIMQSEDKNFSFDNIVKSGGKLYYDVSQESFDKINSLKNIKGIYTYKTQEKKKDDN
                     WKIENMVTSTKGFALVENKDKKVETKEVDKSEKSLEMVINKELENNKDPKIIFERDHE
                     GVYGEGQYDIPEDNLNVKLTLDNKFQKIVRDVLAKEDYKTFKNAGAVLIDSASGEVLA
                     LAQKDESAPNIVLGSGSINGYEAGSIFKILTLEAAMEEKGISLSDKLRCDGLVCKKEK
                     IHGTISIEEAFEVSCNDIFSKLGAQIGTRRLLDFAKEQGVFSLALGLDEKTGMETKGD
                     VPENVSITNLSIGQSMQTSLIQMAGIISTVINEGEYVEPYILKDFENQKGEIKKEFKE
                     LRKNVISKKTAEDLKSVMNSTVLEGTANITKIEGVEIGAKTGTAEALGGELHGWFLGY
                     FKHGEKYYTLGVMVPNIKNVYEDRKPGGGNTAGPIFRDIVLELTKNK"
     gene            14018..14719
                     /gene="sigK"
                     /locus_tag="K8M77_001060"
     CDS             14018..14719
                     /gene="sigK"
                     /locus_tag="K8M77_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HBI7085571.1"
                     /translation="MFMLQYLLELVGSKIFLTGYVTGNSTFPKPLNEKEEKIYLDRLK
                     DGDVEAKRVLVERNLRLVAHIVKKYSSNYQNSKEMDDLISIGTIGLIKAIDSFDTNKG
                     IRLATYAAKCIDNEILMFFRNTKKTKGEVFLQDPIGVDKEGNEICLIDILSSDSDSVL
                     EAVENSLQVKELYKKMSDILSPREKEIIKMRYGLLDGDIKTQREIAAILGISRSYVSR
                     IEKKALKKLNKEFKC"
     gene            14900..15952
                     /locus_tag="K8M77_001061"
     CDS             14900..15952
                     /locus_tag="K8M77_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBI7085572.1"
                     /translation="MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILR
                     PEDTEAYAKEILQDAYEKYDAIGEYDTSYSIHGKGRFRVNIYKQRNSTALAIRVISLD
                     MPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
                     PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAA
                     ETGHLVFSTLHTIGAAKTIDRIVDVFPPHQQEQIKIQLASVLQTIISQQLVETIDGER
                     TASLEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMELANLYREGIITQETA
                     MNSAIDREILSRLLMY"
     gene            16030..17268
                     /locus_tag="K8M77_001062"
     CDS             16030..17268
                     /locus_tag="K8M77_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBI7085573.1"
                     /translation="MSKNIVGLDIGNRNICAAISGENENGEFEVLDFIVKDLQGVSKG
                     KIIDEESVVKTIKECLKELEEKSGHNIRGVYLSLKNNDCRMVENKGYSYTDNDDSLVF
                     KENVEEAYIEGRTLKLSEEECVADSAINSFYTEEFGFVQNPIGIRTERIEIDEDLIIA
                     PKSKIRTLNKIILEAGYEVLGTVSLGFGFKNVFLSKKTETSNVVIIDVGAEETQIYSY
                     KGNTLKDMDYIPLGGRNISKDLAICLSISEEEAERLKLQYSSKYYSIRKDYNNISFEE
                     HILDTYLIHDIIDARLSEIVELVNSKLMERDILNTTDMIILTGDGISYYEHIKERIEY
                     TTDKDVRIFTKKQFLFDNSSIINSISIVKEVYDRLKLICDEELLSGRKVTVDKENKSE
                     NKSVKKRGLSKIMAFLEELF"
     gene            17282..18427
                     /gene="ftsZ"
                     /locus_tag="K8M77_001063"
     CDS             17282..18427
                     /gene="ftsZ"
                     /locus_tag="K8M77_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBI7085574.1"
                     /translation="MLDFDVDIQSFTNIKVIGCGGGGGNAVNRMIQEGLRDVEFIAIN
                     TDKQALTLSHAQNKIQIGDKLTKGLGAGANPEIGKKAAEESRDEITEAISGADMVFIT
                     AGMGGGTGTGAAPVVAEIAKSMGILTVGIVTKPFPFEGRRRMTHAEMGIANLKEKVDT
                     LVTIPNERLLSMVDKKTTLLESFKKADDVLRQGVQGISDLITNPGLINLDFADVRAVM
                     LDKGLAHMGVGYGKGETRAQDAAREAISSPLLETSIVGATGVLLNVTGDSELGLLEIN
                     EAAEIVQEAADPDANIIFGTVIDETLKDEIRITVIATGFEKERQRMGMGAQGVTSGAT
                     QTQREVIVENVEEKIAEQEVAASSQTQQEDRYNDDLEIPMFLRRGRR"
BASE COUNT         7466 a         1950 c         3678 g         5398 t
ORIGIN      
        1 aaatatatat aataatatta ttttaatagt attttatata aaaataagta tttaacttag
       61 tagataaata atcataaaat atataggaat acatagtaat ttagtaaaat agatattaat
      121 acagttggca attatattga caggtaaaga aatttttttt ataatttgcc tataataact
      181 taatgaaaag cgttgacgag aatgagtagg catttatctt ttagtcagag aggaagtggt
      241 tggtgagaac ttcttaaaag agatgttgaa gctatctttg agctataatt tttagagtga
      301 tgtagtttta cataattagg gtggtaccgc ggagcatagt atttcgtccc ttttaattag
      361 gggtgaggtg ttttatgttc tttttttatg tgtgaaacta attataaaat aaaacaatat
      421 ctagagctaa gtatagagtt tgatcttatt ttataaatag cagattaaac ttaaaggcta
      481 aaggataaat acataaaatt tggaggaata aattatgaaa ttcatgggag caaatgaatt
      541 aagagaaaaa tatttaagct tttttgaaag caaagaccat ttaagattac agtcatttcc
      601 gttagtacct aaaaatgata agagtttatt attaataaat gcaggtatgg caccacttaa
      661 accttatttc acaggtttag aagaaccacc aaagagaaga ataacaactt gccaaaagtg
      721 cataagaact ggtgatatag agaatgttgg taaaacatca agacatggta cattctttga
      781 aatgctagga aatttctcat ttggagatta ctttaaatca gaaataattc catgggcttg
      841 ggagtttata acagaaactt taggaattcc aaaggataaa ttatatgtaa ctatatattt
      901 aaatgacgat gaagcttatg atatttggac tagtaaaaca gatgtagatc caagcagaat
      961 attcagatta ggaaaagatg ataacttctg ggaaataggt gtgggtcctt gtggtccttg
     1021 tacagagatt cactttgata gaggagaagg aaaagttgaa actgtagaag aattcttaga
     1081 agcttcagat gctgatagaa tagttgagtt ctggaactta gttttcactc aatttgataa
     1141 agatgaagaa ggaaactaca atgagttagc tcaaaagaac atagatacag gtatgggctt
     1201 agaaagaata gctacaataa tgcaaggtgt agataatatc ttcgaaatag atacagttaa
     1261 aaacatatta aataaagcat gtgaattaac aaatgctaaa tatggagaag ataaagacaa
     1321 agatgtatca ttaagaataa taactgacca tggaaaaagt gttactttct taatatgtga
     1381 cggtgttcaa ccatcaaatg aaggtagagg atatgtttta agaagacttc ttagaagagc
     1441 tgctagacat ggaagacttt taggagttaa aggtatattc ttaaatgaaa tggttgatgc
     1501 tgtagttgaa aattacggtg aagcttatcc agaattaaaa gaaaaagctg attacattaa
     1561 gaaaataatt aaattagaag aagaaagatt taatgaaaca atagactcag gtatggatat
     1621 acttatgagc tatatttcag aaatggaaga aaaaaatgag aaagttttat caggagctaa
     1681 ggctttcaaa ttatatgata cttatggatt ccctctagag cttactcaag agatattaga
     1741 agaaaaagga ttagagttag atatagaaaa ctttaataag gaaatgaaag agcaaagaga
     1801 aagagctaga aatgctagag gagaaagtag ctacatggga agcgaagaaa gtccagtaaa
     1861 caaagtggat gcttcaatag ttactgaatt tgatggatat gttaatttag agcttaactc
     1921 aaaagttata gtactaggaa ataatgaaga atttaaagct gaacttaaag aaggcgaaga
     1981 aggattctta ttaactgata aaactccttt ctatgctgaa atgggaggtc aagttggaga
     2041 tagaggaaat ataacttcag aaactggaat ggcaatagtt acagattgta agaaaaatgt
     2101 tggtggaaaa tttgttcatt acattaaggt tatagaaggt agcttaaaag aaggtcaaga
     2161 agttaaatta tcagttgatg cttcaagaag atctaacata tgtaaaaacc acacagctac
     2221 acacatttta catgaagctt taaaagaaat tttaggagat catgtaaatc aatcaggttc
     2281 atatgttgat gaagaaagat taagatttga ctttactcat tttgcagctt taacagaaga
     2341 agaattagaa aaagttgaat tattagtaaa tgaaaaaata atgactgtat ctgtagttga
     2401 tacaaaggaa atgtcattag acgaagctag aaatagtgga gcaacttgtc ttttcgatga
     2461 aaagtatgct gaaaaagtaa gagttgtttc agttggagat ttctcaaaag aattatgtgg
     2521 aggaactcac gtagctaact caggagaaat tggattattt aagatagttt cagaatcagg
     2581 agttgctgct ggaataagaa gaattgaggc tgttactgga ataagcgcat taaaattcat
     2641 ggaacttaaa aataatatgc ttaaggaagc tgcttcaatg cttaagtgta atgaaaaaga
     2701 tattgctaag agaatagcag ctcaagctca tgaattaaaa gaaaaagata aagaaatagc
     2761 agaacttaaa gctaaattag ttcaaggggc tgaggatgat atcttaaaag acaaagttga
     2821 aataaatgga gttgaattag ttacagcaga attaaaagat gttgatggaa attcattaag
     2881 agatttagct gataaagtta gaaataaatt aaataatgga atagttgttt tagcaagtga
     2941 caatggtgga aaagtaaact tagtagctat ggcaactaaa aattcattag ctaatggagt
     3001 tcattgtgga aaggtaataa aagaagttgc agcagttgta ggcggcggag gaggtggaag
     3061 acctgacatg gctcaagctg gtggaaaaaa tccagaaaat atagctaaag cattagaaaa
     3121 agcaaaagaa gttgtagaat tacttgtaaa atagttgtac ttttatgtaa atatagttta
     3181 taatgagtat aaagtaaagc gtgaataaaa attaatttaa aagataataa tatatataaa
     3241 tatataaatt aaaatatatt tactgtaagg gggtgagctg ctagtaagca gcatgctatg
     3301 agtaacaatt ttgaccacac aatgcagttt gattttagta aaaataagga agatttaact
     3361 aaatcaatat taactgatgt atacaactca ctaaaggaaa aaggctataa cccggttaat
     3421 cagctagttg gatacttaat ttcaggtgac cctacttaca tcactaatta caacggggca
     3481 agagctttag taaggaaact ggaaagagat gaaatacttg aagaagttat aaaatcttat
     3541 ctagagataa aataacaaag agtgcccgaa agggtactct ttcgtttatg taaggagatt
     3601 caatgagaat tttaggttta gatatcggaa gtaaaactat aggagttgca gtaagtgatc
     3661 cacttggatg gacagctcaa ggtgtaacaa ctataaaaag agattgctac actaaagacg
     3721 tagaggcagt catgaaaata tgcaaagaat atggagtaga aactatagtt gcaggtatgc
     3781 caaaaaatat gaatggaaca ataggaccat ctggagaaat ggttaaaaat ttatgtgaac
     3841 aaattgaaaa atcatttgat ggtaaaatag agttttggga tgaaagactt acaacagttg
     3901 ctgctcatag agcaatgcta gaagcggatt tatcaagagc gaaaagaaaa aaaatagtcg
     3961 ataaaatagc ggctacttat atattacaag gatacttaga cagaatatca aaataaaaag
     4021 gagaagttaa aatgaataac gatttacaac caatagtttt agttgatgaa gaaggaatag
     4081 aaacaacatt caacgtagta actaaattag atatcgaaga aaaagaatat ttcttattat
     4141 caccagaagg tgaggaagat gtagttatag ctatgcaagt tgttcaagac gaagatggag
     4201 aagagacttt agctccagtt gagaatgatt ttgaaattga aatgatagaa gaagcttatg
     4261 caactttatt cgcagaagaa gaataacggt aaagtgaggt tttaatatgg caaatacagc
     4321 ttcagtagat tttgatgctt taaaagaaga attaaagaag aagggttata agttaacacc
     4381 tcagagaaga gctatagttg atactataat acaaaatgag ggtaaacact taactgctga
     4441 ggaaatttat gacgaggtta agaaaacttg tccagagatt ggattagcta ccgtatatag
     4501 aacaataatt cttctagaag agatgggtgt aatctataag ttagatttag aagatggatg
     4561 cagtagatat gaacttgctc atgatgatga agagcataga caccatcatc tagtttgtaa
     4621 taaatgtgga aaagtttttg aggttgaagc ggatttatta gacgaactag aagaagagat
     4681 tgaaactaaa tatggctttg aaatattaga tcatacagta aagtttttcg gaatatgcaa
     4741 ggattgtaaa aatgaataga gaaattttct caaatgagga aacctctttt atacataagc
     4801 gtactactac aaaaaagagg agggaaaata tgaaacgtga aaagctaaag gttaaaatta
     4861 tacctttagg tggtttgaat gagataggta agaatctaac tgtaattgaa tttaaagatg
     4921 atataattgt agtcgactgt ggacttaagt tcccagacga agatatgttt ggtattgata
     4981 tagttattcc agacgtatca tatcttgtta aaaatgcaga aaaagttaga ggaatatttt
     5041 taactcatgg tcatgaagat catattggtg ctttaccata cgtgttaaaa aatctaaacg
     5101 tacctgttta tggaacaaaa cttactttag gaatagttga aactaaacta aaagaacatg
     5161 gtttattaag tactacagag cttataagag ttaaaccaag agatattata aaattaaaat
     5221 catcatctgt tgaatttgta aagactaatc acagtattgc agattcagtt gctatagcag
     5281 tgcatacacc actaggagtt gtacttcata ctggtgattt taaagtagac tacactccaa
     5341 ctgatggaga ggtaatggac tttgctagat ttgcagaatt agggagaaaa ggagttcttg
     5401 caatgatggc tgattctact aatgtagaga gaccaggata tacaatgtca gaaagagctg
     5461 taggagaaaa tcttaaaaaa atatttattg gagcaaaagg aagaataata atagctacat
     5521 ttgcttcaaa tattcatagg atacagcaaa ttgttgaagc cgcagaaatg acaggaagaa
     5581 agattgctgt ttcaggaaga agtatggaaa atatagttca ggttgccata gaactaggat
     5641 atttaactat agataaagat tcttttgtta gtatagattc tataaataaa tatccaaatg
     5701 agcaagtgac tataatcact acaggaagcc aaggagagcc aatgtcagca ttagctagaa
     5761 tggcatcttc agagcataaa aaagttaata tcattgaggg agatacagta atactatctg
     5821 caacaccaat acctggtaat gaaaaattgg tttctaaagt tattaaccaa cttttcaaaa
     5881 agggagcaga ggtagtctat ggaaaattag cagatgttca tgtttcaggt catgcttgcc
     5941 aagaagaatt aaaacttatg caagcgcttg ttaagcctaa attctttata ccagttcatg
     6001 gtgaatatag acaccttaag cagcatgcag agttagctgt tgatcttgga ttatcagaaa
     6061 agaattttat gatagctgaa aatggggacg ttatagagat aactagagat tcaatcaaga
     6121 agaacggatc tgttacttca ggtcaaattt ttgttgatgg acttggagtt ggtgatgtag
     6181 gaaacatagt tttaagagat agaaaacatc tttcacaaga cggaattctt acagtagttg
     6241 ttactatttc aaaagaggct gcttcagtgg tagcaggacc agatattata tcaagaggtt
     6301 ttgtttatgt gagagaatca gaagatttaa tggatgaagc taaagagata gtaaaatgta
     6361 ttttaagaga ttgtgaaaag aaaggaatat gtgattgggc tactataaaa tctaacataa
     6421 gagatggcct tagaagtttc ctttatgaaa aaactaagag aaaaccaatg atattaccaa
     6481 taataatgga aatataaaaa attagatgat aatatagtat gtaactgttt aagtaaaaaa
     6541 tgttgtgttg acttttttta agcttaacag tagaatctaa taaggtaaag aaaattgggt
     6601 acttaaatag tatccaattt tttatgcaca taaaattgga ggaataaata taatgagtaa
     6661 cgaatttaca agaaatgata ttagaaatat agctataatc gctcacgttg accatggtaa
     6721 aacaacttta gtggatgcat tattaagaca aagtaatgtt tttagaacaa acgaaaaagt
     6781 agaagagaga gtaatggact caaatgactt agaaaaagaa agaggtatta caatactttc
     6841 aaaaaataca gcagttcatt atgacggggt taaaataaat ataatagata caccaggaca
     6901 cgctgatttc ggaggagagg ttgaacgtgt acttaaaatg gtagaaagtg ttttacttgt
     6961 agtagactca tatgaagggg ctatgccaca aacaaaattc gttttaagaa aagctcttga
     7021 gttaggatta aaaccaatag ttgttataaa caaaatagat aaaccagatg ctagaccaga
     7081 agaagttatt gacgaaatat ttgaattatt cttagagctt ggtgcagatg atgagcaatt
     7141 agatttccca atagtttatg cttcagctag agatggagtt gctacaatta acatagatga
     7201 gccaaaagat aacatgaaag acctattcga tactataatc gaaaaagttg aatcaccaaa
     7261 gggatctata gaagatagct tgcaaatgtt agttacaact ttagattcaa gtgaatatgt
     7321 tggtaaaata gctataggta aaataactag aggagtagct aagaaaaacc aacaagctgc
     7381 tgttgtaaga caagatggaa cagtaaataa attcaaaata tcaagtcttt atactcatga
     7441 tggattaaag agaatagagg ttgaggaagc acaattagga gatatagttg caataagtgg
     7501 tatatctgac gtaaatattg gagaaactat aacagatgca caaaatccag aaggattacc
     7561 atttgttaaa atagacgagc ctacattaaa catgaacttt atggttaatg actcaccatt
     7621 tgctggtaga gaaggggatt tcgtaacttc aagacattta agagatagat tattaaagga
     7681 attagaaact aacgtaagtt taaaagttaa agaaataact ccagattgtt tcgaagtaag
     7741 tggtagagga gagctacatc tttcaatact tatagaaaca atgagaagag aaggatatga
     7801 attccaagtt tctaagccaa acgttataac aaaaatagat gaaaatggtg ttaaggttga
     7861 accaatagag cacttaacta tagacgttcc agaagaattt atgggacctg ttatggaaaa
     7921 attaggacct agaaaagctg aaatggtaaa tatgacttca gctgttaatg gatactcaag
     7981 attagagttc aaaatacctg caagaggact tataggattt agaaatgaat tcatgacaga
     8041 tacaaaaggt aacggaataa tgaaccatgt atttgatgga tttgaaaaat acaaaggaga
     8101 gatcccagga agaagtagag gatcaatcgt agtatttgaa tcaggagaag cagttacata
     8161 tggattattt aatgctcaag aaagaggaac tttattcata gagccaggta cagaagttta
     8221 tgctggaatg gtttgtggag aatgttcaag agcagatgac atagacgtta atatttgtaa
     8281 gaagaaacaa ttaactaata caagatcatc tggagctgat gatgctttaa aattaactcc
     8341 agtaagacaa atgtcattag agcaatgttt agaattcata aacaatgacg aattagttga
     8401 agttactcca gtaaatgtaa gaatgagaaa gagaatctta gacagtgctg agagaaagag
     8461 agctataaat agaagtaaaa aataataaat tttcttataa ttttatttta taactttggg
     8521 tacataaaca aaattagtaa aacaagtttg tttttatgag aaaaagattt ataattagat
     8581 aaatggcttt atggccattt atcttaaaat atgagggggt actactgtga aaaagctaaa
     8641 aaactctaaa agtatggttt tgatatccat attcataatt ttacttgtga ttaacttagc
     8701 tgtatttgta gttaaatata actccataaa gcgaagccct ttacagtcta acaaagccga
     8761 tataaccttt aaagttaaag acggagagtc actaaatgga ctttttgaaa gattaaacaa
     8821 tgaaaatgta cttagaagtt cctttttttc taagatatat ataaagttta ataacgtaga
     8881 agaaactata aaaccaggta catatactgt aaatagtgac ataagtttta aggactttat
     8941 aagtgtacta actgatggaa aagtatcaga ttataagtta acatttccag agggatatac
     9001 agtagaagat atagctaaga aattagatga atctaaggta tgcacaaaag atgagttttt
     9061 aaaagtagaa aaagagtatc cattaccatc ttatataaaa cctaataatg aaagaaaata
     9121 tgagttagaa ggatttttat tcccagacac atatgctatt cctaaaggca caacaccaaa
     9181 acaaataatt gaaatgatgc ttaacaggtt tgaaggggta attagtgaga tacaaagtga
     9241 actaggcatt actatttcaa aggaagagta tgagaaatat gtaatagtag cttcaatggt
     9301 tgaaaaagag gctagggatg atagtgagcg tgcagaaata gcatctgtta tatataatag
     9361 actacaaaaa ggtatgcctt tacaaatcga tgctacagtt ttatatgctt taggagagca
     9421 taaagatact gtgctttata aagatttaaa agttgattca ccatataata catataagat
     9481 taaaggactt ccagtggggc caatatgtaa tcctggaaaa ccttcacttt tggctgccat
     9541 aaaaccagct aaaacagact acatatatta tttattgaat ccgtcaaata ataagcacta
     9601 ttttactaat aattacgaag atttcatagc taagaagaaa gaatttggat actaaaattt
     9661 ggaggagcaa atgagtggag taacctttga atatatggaa aagtatctta gagatcttat
     9721 tccagataga aatgacgaat taaaagaact agaagatttt gcaaaagaaa acaaggtacc
     9781 aataatacaa aaggaaggtg ctaagttcct tgagtttatg gtgtctatga ataaaccaaa
     9841 gaaaatatta gaattaggca cagctatagg ttattcttca atactgatga acaaagcttg
     9901 tggtggagaa tgtcatatta ctacaatcga gagagatgat aatatgataa attatgctaa
     9961 agagaatatt aaaaaatttg gattagagga aaaaatcaat attttacagg gagattgttt
    10021 agaagtttta gagagtcttc aggatgaatt tgatatgata ttcatggatg caggtaaagg
    10081 acattataat cattttttac cagaatgttt aagactttta aaaaaagatg gaattataat
    10141 agcagataat gttttgttta gaggaatggt tgcttctgat gacttagtaa agagaagaaa
    10201 aataactatt gttaagagaa tgagaaagta tttggatatg gtctcaaagg atgaaaattt
    10261 aataacaact gttattccta tgggagatgg aattgcgtta actaagagga ggtagcaaac
    10321 atgagaaaac cagaaatatt agcaccagca ggtagcttag aaaaattaaa gacagcaatt
    10381 gattttggag cagatgcagt ttatattgga ggaagcaaac ttaacctaag agctttcgct
    10441 gataacttta caaatgaaca gattgcagaa ggtgtaaagt atgctcatga cagaggaaga
    10501 aaagtttatg ttactatgaa tgtatttcct cacaatgcag atttagaagg gttagaagaa
    10561 tacattgtag gacttaatga tttaaatgta gatgcaataa tagtttcaga tccatcaata
    10621 ataatgactg caaaggaagt tgcaccagat ttagaaatac acttaagtac tcaagcaaac
    10681 aatgtaaact ggaagtcagc taagttttgg catagcttag gagttaagag aattgtttta
    10741 gctagagagc ttagttttaa agaaattgaa aaaatacacg agaatttacc agaagattgt
    10801 gatttagaag catttgttca tggttcaatg tgtatggcat actcaggaag atgtttaata
    10861 tcaaactaca tgacaggaag agactcaaat agaggagctt gttcacaagc atgtagatat
    10921 aagtattatt taatggaaga aaagagacct ggagagtatt tccaagttat agaggatgat
    10981 aaaggtacat acataatgaa ctctaaggat ttatgtatga tagaatatat accagagctt
    11041 gttaagagtg gtatatactc atttaaaata gaaggaagaa tgaagagccc atactatgtt
    11101 gctgcaattg ttaaagctta cagagaagct ttagataagt attgggatga tccagaagga
    11161 tatgaatttg atcaaaaatt aatggataat cttttaaagg ttagtcatag aagatatcac
    11221 acaggatttt actttggaaa atcaggagag caggtttatg aatcatcatc ttatataaga
    11281 gattatgata ttgtaggagt tgttagagat tataacgaag aaactaaggt agcaactata
    11341 gaacaaagaa acagattatt cgaaggggat aaagtagagg tattaactcc agtaggagat
    11401 tactatgaaa tccaaatgaa tgatatgaag gatgaaaaag atgaaaaaat agatgttgct
    11461 aacaaagctc aaatgatatt caaggttaaa atagataagc ctgtaaaggt aaatgatatg
    11521 ttaattaagt gcaaggaggc aaacagctaa tgaagagacc aatttttata ggaataacag
    11581 gaggcactgg ctcaggtaaa agtacaatag ctaaggagat atatagacaa tttggtgaag
    11641 aatgtatagc aatgatagag caagattcat attataagga tcaaagtcat ttatcaatgg
    11701 atgatagagt aaaaactaac tatgatcatc caaatgcttt tgataacaat cttttagttt
    11761 cacatttaga atcactttta aatggacatt gtatacaaaa acctagttat gacttctcta
    11821 tacataatag aattgaagat acaactaagg ttgagccaaa agaaattgtt attgtagaag
    11881 gaatattaat actagaagat ccaagaataa gagagcttct agatataaag atttacgttg
    11941 atacagatgc tgatgttaga atcataagaa gaatggtcag agacataaat gaaagaggaa
    12001 gaactataga gtctgttata aatcagtact taaatgtagt aaagcctatg cataatcaat
    12061 ttacagaacc tacaaaaaaa tttgctgata taataatacc agaaggtgga cataataaag
    12121 ttgctataga tattattgtt gcaaaaataa aagaggtttt aggaaaatac gaataatatt
    12181 ttaaaaatgt atattattat tttacttaaa taagagataa aagtagttat ttcttattga
    12241 ataaaacacc cataacttgg ctagaattta gccaggaggg tgtttttatg tttaatagga
    12301 aaagaaaaat tgaaattaaa aatagattaa taagcttagt ttctttattg ttattagctt
    12361 tactattaat tgatatgagg atttattatg tacagaaaaa gtatagtaca aaagaaacag
    12421 gaaatttcaa aggaaatcac aaacaatatg aaaatatttc tgaccttaat tattcacttt
    12481 tagatataaa tggagaaagc atatttcaat atgatactaa atacaaagtt gttatagatt
    12541 ctatggcttt tagattaaat aatttaaatc aaaatttaga gaatataatg gcctttaatt
    12601 atattatgca atctgaagat aagaattttt cttttgataa tatagtaaaa agtggaggaa
    12661 aattatatta tgatgtttca caggaaagtt ttgataagat aaatagtttg aaaaatataa
    12721 aaggaattta tacttataag acacaagaaa agaaaaaaga tgataattgg aaaatagaga
    12781 atatggtaac ttctactaag ggttttgctt tagtagagaa taaggataaa aaagtagaga
    12841 caaaagaggt tgataaatca gaaaaatctc tagaaatggt aataaataag gagttagaaa
    12901 ataataaaga tcctaaaatc atttttgaaa gggatcatga aggagtatat ggagaagggc
    12961 aatatgacat tcctgaggat aatctaaatg ttaagttaac tttggacaat aaatttcaaa
    13021 aaatagttag agatgtttta gctaaggaag attataaaac ttttaaaaat gcaggagcag
    13081 tacttattga tagtgctagc ggtgaagtat tagctttagc tcaaaaggat gaatcagctc
    13141 caaatattgt tttaggatca ggtagcatta atggttatga ggcagggtca atatttaaaa
    13201 ttttaacttt agaggctgct atggaagaaa agggtattag cctttctgat aaattaagat
    13261 gcgatggatt agtatgtaaa aaggaaaaaa tacatggaac aataagcatt gaagaagctt
    13321 ttgaggtttc atgtaatgat attttttcta aattaggagc tcaaatagga actaggagat
    13381 tattagactt tgcaaaggag caaggagttt tttctttagc tttaggatta gatgaaaaaa
    13441 caggtatgga gactaaggga gatgttcctg aaaatgttag tataacaaat ctttctatag
    13501 gccaatctat gcaaactagt ttaattcaaa tggcaggtat aatatctact gttattaatg
    13561 aaggggaata tgtagagcca tatatattaa aagattttga aaaccaaaaa ggagagataa
    13621 aaaaagaatt taaggagtta agaaaaaatg taatctctaa gaagacagca gaggacttaa
    13681 aaagtgtaat gaactcaacg gttttagaag gaactgcaaa tataactaaa attgaaggag
    13741 ttgagatagg agctaaaact ggaactgctg aagctctagg gggagaatta catggatggt
    13801 ttttaggata ttttaaacat ggagaaaagt attatacttt aggagttatg gtgccaaata
    13861 taaaaaatgt ttatgaagat agaaagccag gaggtggaaa tactgcaggt cctattttca
    13921 gagatatagt tttagaactt acaaaaaata aatagattta gcaatatttt aactatcaac
    13981 tcatattata gtaataagca ttaatagggg gaacgatatg ttcatgttac aatacttatt
    14041 agaattagtt ggtagtaaaa tatttttaac tggctatgta acaggaaatt ctacttttcc
    14101 aaagccttta aatgagaagg aagaaaaaat ctatttagat aggttaaaag atggagatgt
    14161 tgaggcaaaa agagtactag ttgagcggaa tttaagacta gtagcacata tagttaagaa
    14221 gtattcttca aattaccaaa actcaaaaga aatggatgat ttaatatcta taggaacaat
    14281 tggtttaata aaggctatag attcttttga cactaacaag ggaatacgac ttgcaacata
    14341 tgcagctaag tgtatagaca atgaaatact tatgtttttt aggaatacta agaagacaaa
    14401 gggagaagta ttccttcaag atcctatagg agtagataag gaaggtaatg aaatttgtct
    14461 catagatatt ttaagtagtg attctgattc ggtattagag gctgttgaaa attcattaca
    14521 ggttaaggag ctttataaaa agatgtccga tatcttaagt cctagggaga aagaaataat
    14581 taaaatgaga tacggtcttt tagatggtga tataaaaaca cagagggaaa tagcggcaat
    14641 tcttggtata tcaagatcat atgtatctag aatagaaaaa aaggcattaa aaaaattaaa
    14701 taaggaattt aaatgttaaa tgatacttaa taaattgtat aaaaaaaaat aagttttata
    14761 aaattatgga attgatatat catatggttt ataatattta ttatacgata aaaaaggtgg
    14821 attcttctat aaataatttg ttcatttctt tatggagtga acaaattatt tattaagaat
    14881 taagtttagg aggagattta tgcaaagttt agcagaactt ttagagttaa cagttaaaga
    14941 aggagcatct gatttgcatt taacagtagg tatttctcct ataataaaag ttaatggaaa
    15001 attagtaagg ttagaacatg aaatattgcg ccctgaagat actgaagcgt atgctaaaga
    15061 gatattacag gatgcatatg aaaagtatga tgctattgga gaatacgata catcatattc
    15121 aatacatggt aaaggtagat ttagagttaa tatatataaa caaagaaata gtacagcttt
    15181 agctataaga gttatatctt tagatatgcc aactttagat agtttaggat atcctgagac
    15241 tttaaaggat atatgtaatc ttaaaagagg acttgtatta gttacaggac ctactggaag
    15301 tggtaaaagt acaactttag cagctttaat caatgaaata aatagtaata gagaatctca
    15361 tataataaca atagaagatc ctatagagtt tcttcataaa cataataaat ctattgtaaa
    15421 ccaaagagag ataggaaagg atactttaag ttatgaaaga gccttaaaag cagctcttag
    15481 agaggatcct gatgttattt tgattgggga aatgagagat ttggagacta tttcaacagc
    15541 tataacagcg gcagagactg gacacttagt attttcaact cttcatacta taggagctgc
    15601 taagactata gatagaatag tggatgtttt tccaccacat caacaagagc aaataaaaat
    15661 acagttagct tctgttttac aaacaattat atcacaacaa ttagtggaaa ccattgatgg
    15721 agagagaact gcgtcattag aaataatggt tgctacacca gctataaaaa acttaataag
    15781 agaaggaaaa actcatcaaa tagaatcttc tatccaaact ggaagtaaat atggaatgag
    15841 aactatggac atggaacttg ctaatctgta tagagaaggt ataataacac aagaaacggc
    15901 aatgaattct gctatagata gggaaatttt aagtcgatta ttgatgtatt aaatatagag
    15961 gtaatttgaa tttttaagag aatatatata ttatcacaat ttttagatag ggaggtacac
    16021 atagaaaaaa tgtctaaaaa catagtgggt ctagatattg gaaacagaaa tatttgtgca
    16081 gcgatatcag gtgaaaatga aaatggagaa ttcgaagtat tagattttat agtaaaagat
    16141 ttgcaaggtg tatctaaagg taaaataata gatgaagagt ctgttgtaaa aactattaaa
    16201 gaatgtctaa aagaactaga agaaaaaagt ggacataata ttagaggggt ttacttatca
    16261 ttaaaaaata atgattgtag aatggtagaa aataagggat attcttatac tgacaatgat
    16321 gattcattag tttttaaaga aaatgtagaa gaagcttata tagaaggaag aacattaaag
    16381 ctttcagagg aagaatgtgt agcagatagt gcaataaatt cattctatac tgaagaattt
    16441 ggttttgttc agaatcctat tggaattaga actgaaagaa tagaaataga tgaagattta
    16501 attatagctc ctaaatctaa aattagaact ttaaataaaa ttattttaga agcgggatat
    16561 gaggttttag gaacagtttc attaggattt ggatttaaaa atgttttttt aagtaaaaaa
    16621 acagaaactt caaatgttgt aattatagat gtaggggcag aagaaacaca aatatatagt
    16681 tacaaaggga atacactaaa ggatatggat tatataccat taggaggaag aaatataagc
    16741 aaagatttag ctatatgttt atctatatca gaagaggaag cagaaagatt aaaattgcaa
    16801 tattcttcaa aatattatag tattagaaag gattataata atatttcctt tgaggaacac
    16861 atattggata cctatttaat tcatgatatt atagatgcaa gattaagtga aatagtagaa
    16921 ttagttaata gtaaattgat ggaaagggat attctaaata ccacagatat gataatatta
    16981 actggtgacg gtattagtta ttatgaacat attaaggaaa gaatagagta tacaactgat
    17041 aaggatgtaa gaattttcac taaaaagcaa tttttatttg acaattcttc aataattaat
    17101 tcaattagta ttgttaaaga ggtatatgat aggttaaaat taatatgtga tgaagaatta
    17161 ctttcaggaa gaaaagtaac agtggataaa gaaaataaaa gtgaaaataa aagtgtaaag
    17221 aagagagggc tttccaaaat aatggcgttc ttagaggagc ttttttaaag gaggaattag
    17281 tttgttagat tttgatgtag atatacaatc atttactaat attaaggtaa ttggatgtgg
    17341 aggcggaggc ggaaacgctg ttaatagaat gattcaagaa ggattaagag atgttgaatt
    17401 tattgctata aatacagata aacaagcctt aacactatct catgcccaaa ataaaattca
    17461 aataggcgat aaacttacaa aaggactagg tgcaggggca aatcctgaaa taggaaaaaa
    17521 agctgctgag gaaagtagag atgagataac agaagctata tcaggagctg atatggtatt
    17581 tataactgct ggtatggggg gcggtactgg tactggagct gcgccagtag tagctgaaat
    17641 agctaaatca atgggaatat taacagtagg tatagttact aagccattcc catttgaagg
    17701 tagaagaaga atgactcatg ctgaaatggg tatagcaaat ttaaaagaaa aagttgatac
    17761 attagttaca atacctaatg aaagactttt atcaatggta gataagaaaa caactttatt
    17821 agaatcattt aagaaagctg atgatgtttt aagacaaggt gtacaaggta tatcagatct
    17881 tataactaat ccaggtttaa taaacttaga ctttgcagac gttagagctg ttatgttaga
    17941 taaaggttta gcacacatgg gtgttggata tggtaaagga gaaacaagag cacaagatgc
    18001 tgcaagggaa gcaatatcat ctccactatt agaaacatca atagttggtg ctactggagt
    18061 tttattaaat gtaacaggag attctgagtt aggtttatta gaaataaatg aagctgcaga
    18121 aatagttcaa gaagctgcag atccagatgc taacataata tttggtactg ttattgatga
    18181 aacattaaaa gatgaaataa gaataactgt tatagctact ggatttgaaa aagagcgtca
    18241 aagaatggga atgggagctc aaggagtaac aagtggagct actcaaactc aaagagaagt
    18301 tatagtagaa aatgttgaag aaaaaatagc tgagcaagaa gttgctgcgt cttcacaaac
    18361 tcaacaagaa gatagatata atgatgattt agaaatacct atgtttttaa gaagaggtag
    18421 aagatagtat ttttaaaaat aaaagcactt atggtaatta attactataa gtgcttttat
    18481 ttttacttta at
//
LOCUS       DAEVGI010000035        17652 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEVGI010000035 DAEVGI010000000
VERSION     DAEVGI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 17652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17652
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.035"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(6..851)
                     /locus_tag="K8M77_001064"
     CDS             complement(6..851)
                     /locus_tag="K8M77_001064"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085575.1"
                     /translation="MGKKILKLIMIVIVSISIISAIIILLINWKSIEGEWIGFWGSII
                     GSILGGVVTLVALRKTIKSETIARNEDRRLQVIPYLQYNIIQEEPKSKLWRYIILQPN
                     IKIVDEVIKPGVPYEYDLFFYIDIENVGMNSAVNLSILEIKYCGITDYSYIDYKIMKV
                     GEKIHLSFRALVNYQGKFSINRDGCGRVPVEIKLAYTDLIENYYEQDIVVDCCWSSSY
                     YIDEETNVKTDIKYNYYVSHGINDISSPRYIKEKNIMSERDKKMKMFNQKNKYIRKII
                     SKIKK"
     gene            complement(893..2014)
                     /locus_tag="K8M77_001065"
     CDS             complement(893..2014)
                     /locus_tag="K8M77_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBI7085576.1"
                     /translation="MELDLISSMHRAFYFHQAEQSKEGFQWEQWQRTKLREIEKYRKR
                     NKKLVEEYNKPIQEAINREIQGNFTKGQENTEKLIDEVKIKFPENIKEPQTVREYIAK
                     ELGKKATPQVEENFFGISEKKLNALQETVTNDLKKAQMSVLRKMDDVYRQIIFKTHVY
                     LQSGTKTINQAIDMATKDFLEKGINSITYKNGKQVNIASYAEMCLRTASQRATFLGEG
                     KKRDEYGIHLIVVTAHANTCKMCEPWQGKVLIDDIFSHGTKEDGDYPLLSEAVGKGFL
                     HPNCRHTLATYFPGITRIPTIPDGKEAIKLYEAEQKQRYYERQLRKWKRFKAGNCDEE
                     NKEIASKKVKELEKVLKDHLESNKELRRNQYREYALIYN"
     gene            complement(2127..3647)
                     /locus_tag="K8M77_001066"
     CDS             complement(2127..3647)
                     /locus_tag="K8M77_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HBI7085577.1"
                     /translation="MGWFKSMLTKAAIKYLNVQPALTNPITIQEAYTFETNVIRNKLW
                     YRGEPYELDQFFKNISSDPVNKARFWSAVPSEDLSIRKIHSGLPAMIADKLSDIVVAD
                     LDSIEVTGENYNTLWEEIRKDNKFNNMLGDIIATTLVSGDGAFKLSIDTEISKYPIIE
                     FFDGDKVEYITQRGRLKEIKFYTFYTKNNRQYKLSETYGKGYINYNLYDSNGNEVSLN
                     TLYETRELADVTYKDDFIMGVPLMFFKSPKFEGRGKSIFDNKSDTFDALDEVISQWID
                     AIRDGRVQKYIPEDLVPKDINGNLMKPNPFDNRFLKVGSSLAEDAKNEIDMKQANINY
                     EAYVESYSNAIDMCLQGIISPSTLGIDLKKTDNAEAQREKEKTTLYTRGKMVDILTEV
                     IPELVNIILKTNDVLNKKNTGEYEVSIVFGEYASPSFDTVVETVGKAKTYGVMSIEQC
                     IEEMYGDTWTDEEKEEEIQRIKKQNGYLVAEEPKTVDDYDMSYTDDNEVETDGQEG"
     gene            complement(3650..4999)
                     /locus_tag="K8M77_001067"
     CDS             complement(3650..4999)
                     /locus_tag="K8M77_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HBI7085578.1"
                     /translation="MSDEYKLSEKYLAFLKHRAPVEALEGTTAAGKTTVGILKFMLMV
                     AESPKKMHVIAAKTTGVAEKNLIQKEYGITDVFGDLVKYNGNGDKDNKIPHIRYITSN
                     GEKIIYILGYDNVDKWKMALGSQFGCVLIDEVNTASIEFVREICTRNDYLMMTLNPDD
                     PNLPIYSEFINCCRPLEKYKKDVPVEILGQLNSEEKPNWTYWFFTFYDNASLSEEAIE
                     KKKTSAPKGTKLYKNKILGLRGRATGLIFSNFERKNNVISKEKAKGMKFVQFTAGLDT
                     AYSQNSPDTFAFTFLGITDKKELVILDEEVYNNKDLETPLAPSDIAPKYFKFLEKNRK
                     EWGFARDVFVDSADQATIMELKKFKRTNPCMYNFINSYKKVTILDRIHLMLGWINTND
                     KVFYYVVDTCKEHIRELECYSWKEDKYEPEDANDHTINSSQYAWIPFRKLIGDYKEE"
     gene            complement(4992..5459)
                     /locus_tag="K8M77_001068"
     CDS             complement(4992..5459)
                     /locus_tag="K8M77_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBI7085579.1"
                     /translation="MKLTPKQKAFAEYYIETGNATESAIKAGYSRKTARVIGQENLLK
                     PALKSYIDEKMKELESKRIAKAEEVLEYLTRVLRGEETEQVVVTENIGDFMSEAKVVD
                     KEISAKDKIKAAELLGKRYRLFVDKVEKDSNVNVNSTTKLDSILNQLKDDDDE"
     gene            complement(5571..6284)
                     /locus_tag="K8M77_001069"
     CDS             complement(5571..6284)
                     /locus_tag="K8M77_001069"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBI7085580.1"
                     /translation="MKEEEILMFADLIDSLAKQNPNYGIDLFFKTPDTTKKEAFEIIY
                     RIEEIDRIMPSSTRPEKGKLSFEKGELLEKLAKKVLNIRNIFSVKERVVCDSNEIDLL
                     LQPATNNAIYEKLLPEYLRKDCLVECKNHKKSIDVTLIGKFYSLMRYKKVKFGFMVTN
                     KEITGQSPWEDGVGLTKKLYLRDDTFIINITIKMIKEALESDTSIINVIKKEVNEIIY
                     HTKFEEDITPHPAECLMKQ"
     gene            complement(6286..6651)
                     /locus_tag="K8M77_001070"
     CDS             complement(6286..6651)
                     /locus_tag="K8M77_001070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085581.1"
                     /translation="MLLDLLEKLQKILENYNPELTEKQIKDLSEVLEFHNHGILPASV
                     VKRILKISYKETHELMVYLMTKDMLKPKYKVYCENDMVTGAVKIYDDPAQVPVSFCDR
                     CDKGCSLIKNLVVEFEVCV"
     gene            complement(6602..7690)
                     /locus_tag="K8M77_001071"
     CDS             complement(6602..7690)
                     /locus_tag="K8M77_001071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085582.1"
                     /translation="MDLKEYKQIIKDTVFLDFPNHLKQKLINDIKSNNVGKYGTKEHE
                     GVVLLTGMADHNERINTCLDKNNLTLKLFDLLEIKKGYRYSIVYKFDNINFDELRDKL
                     MEVATNLDSEYYNNYSNSINNEEFQYLENDDYFFIKFHKYLNELDKKTVSMKCLRYPI
                     VFVFHKNINLFEVRFDRLSFNSDYSFHHRTIEARLAQLQSIYEFKYEYFDMENTIREI
                     VNNHKAFVEEIIWSFETAKSKGLTLRVGEDGVMPFLGDLEVLVNELRKKYNCDDVTKC
                     LEEIENYMNQTKQFANEKFRILSWLKYIEKGNTITLEKTIDLKIIFNYNKNEYSLLNI
                     YDNEINDMERISHVIRFIGKIAKDIGEL"
     gene            complement(8042..8127)
                     /locus_tag="K8M77_001072"
     tRNA            complement(8042..8127)
                     /locus_tag="K8M77_001072"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8093..8095),aa:Ala,seq:ggc)
     gene            complement(8421..8894)
                     /locus_tag="K8M77_001073"
     CDS             complement(8421..8894)
                     /locus_tag="K8M77_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HBI7085583.1"
                     /translation="MDKTLFKKTENLLRNYNTLETEIKLIEAEIKDIQESYTGCGAIG
                     YNEKSGPTNKFSSMVEDEVIRKEKDLFYLKRDLDYKVRLKRRIDLAIQTLRTKEEKDL
                     IRLRYINQPKVSWGTVARHLKYNKDYCRKELRPKVIKQIADFVFYNPGVQERFII"
     gene            complement(8904..9215)
                     /locus_tag="K8M77_001074"
     CDS             complement(8904..9215)
                     /locus_tag="K8M77_001074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085584.1"
                     /translation="MREMKLVRGEVTITIQVATLVDRGLDEEEMWEVVSELANNETVD
                     IDSDVELFDITNINLNEKLSERYENESLDCDDWQELLKKADKEEDEIIEILPGQIGFN
                     I"
     gene            complement(9215..9640)
                     /locus_tag="K8M77_001075"
     CDS             complement(9215..9640)
                     /locus_tag="K8M77_001075"
                     /inference="COORDINATES: protein motif:HMM:NF021182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085585.1"
                     /translation="MSRELKFRVWDKTSDSMLYQDDFERVELDTKNKMVTLIAEEESD
                     KSHYVLDYEDGIEAEILQYTGLKDKNGKEIYEGDILINTNKSKLNLGIENQKYLIVYR
                     ALGFGLKPLFKGMALKFNYTDLELIGNIYENPELLEGEE"
     gene            complement(9653..9835)
                     /locus_tag="K8M77_001076"
     CDS             complement(9653..9835)
                     /locus_tag="K8M77_001076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085586.1"
                     /translation="MEVLEGQISAFDILQNRVIKQTEEYEIVKYGEVYRIHFFDKKDR
                     DKCRVTKNFEYVKRYL"
     gene            complement(9849..10208)
                     /locus_tag="K8M77_001077"
     CDS             complement(9849..10208)
                     /locus_tag="K8M77_001077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085587.1"
                     /translation="MKAYRVYLQEMWIEYCIEYALSYDTALRIFNNKLRKLMRDTKDD
                     WCDKVDFGEEVLSFRDQLKWHEDIEIVFRKNPIIMYKDKERLKADVYHWEEPSYEYGD
                     SDIKNQAIILEEIEINE"
     gene            complement(10397..10579)
                     /locus_tag="K8M77_001078"
     CDS             complement(10397..10579)
                     /locus_tag="K8M77_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085588.1"
                     /translation="MELRLISSNRTELKVSVKRNKLLIEQLKFIGAKPTKESSRYCYF
                     EFEGDMKKTQSMLGLY"
     gene            complement(10590..10985)
                     /locus_tag="K8M77_001079"
     CDS             complement(10590..10985)
                     /locus_tag="K8M77_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI7085589.1"
                     /translation="MKVVVTGKIRGKARPRVCRGHAFTPKDTVEYEKLVRDCYQEQDG
                     RYLEGSIKVLIVAYYKIPKSYSKKRVQAIRDRLEKPRKKPDVDNIGKIILDSLNGIAY
                     KDDAYVADFRVIKEYTEELERVEFELSEV"
     gene            complement(10989..11171)
                     /locus_tag="K8M77_001080"
     CDS             complement(10989..11171)
                     /locus_tag="K8M77_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085590.1"
                     /translation="MVKINSIRELTKHSDKLPEEVALDIQYRIGDWIVSGGSYEDDYI
                     KQQFRYAERVINSKDR"
     gene            complement(11165..11338)
                     /locus_tag="K8M77_001081"
     CDS             complement(11165..11338)
                     /locus_tag="K8M77_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085591.1"
                     /translation="MKVNLLDMYTPHELGIMTYKDIVAKREKKDKEVYAYLKCRDIGL
                     RKRWSVKVGGYRW"
     gene            complement(11353..12084)
                     /locus_tag="K8M77_001082"
     CDS             complement(11353..12084)
                     /locus_tag="K8M77_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI7085592.1"
                     /translation="MTTTRSMEYKCPLCKDTGFILEYREHLQPLAKECSCKVNRDIER
                     AWSEFGVKPSQAKRISEYKPYDKLTENAKKKSVDYILNFENKQENNWFLLMGQPGAGK
                     SHLAIGIGVALFKEKHKKVIYMPYTEAIMGLKGNAKDFEKYTALLEKYKNAEVLVIDD
                     LFKDKVRNGNVIADMTEIDMKHIYPLLNYRYYNDLTTIISTECTPEMLEQLDGALAGR
                     IMERVDKNIVIFHGEEFNYRMKNFR"
     gene            complement(12161..12931)
                     /locus_tag="K8M77_001083"
                     /pseudo
     CDS             complement(12161..12931)
                     /locus_tag="K8M77_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12944..13813)
                     /locus_tag="K8M77_001084"
     CDS             complement(12944..13813)
                     /locus_tag="K8M77_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBI7085593.1"
                     /translation="MATASSLKNQLAKKGTGNSLSIGNTVKGLMKNPSIKKRFEGVLK
                     EKAPQYMSSIVNLVNSDTNLQKCDGMSVIASCMVAATMDLPVDKNLGYAWVVPYGNRA
                     QFQMGYKGYIQLALRTGQYKAINVVEIREGELVSWNPLTEEIEVDFSKRESDAVIGYA
                     GYFKLINGFEKTVFWTKEEVNNHANKFSKTINSKNSVWKSNFDAMAKKTVLRNLLSKW
                     GILSIEMQKAYTADEGLINKEFMDDVENVQANIEDIQEENDGVVDVDYTVDSNGEILE
                     GQENMFEGTPLEE"
     gene            complement(13816..14727)
                     /locus_tag="K8M77_001085"
     CDS             complement(13816..14727)
                     /locus_tag="K8M77_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBI7085594.1"
                     /translation="MEDKLEWLRERQKGIGGSDVGAIMGVSQYKTAFNIYLEKTEPII
                     KLEKQSEAAYWGDQFEEVVAKEFERRTGKKVRRDRRHFQNKDYPFMVANIDRRVVGED
                     AILECKTANQYLAKEWESEEVPPSYLLQVQHYLAVTGASKGYIAVLIGGQKFIWKEIN
                     RDEELINIIIEAEKEFWKMVQDKTPPALDGSSAAEKWVNEKYKNVNEGETINLDSSWR
                     ELLNQRQELKEYRDDVDKQIKEIENQLKQNIKNAEYANAPGYSISYKSVSQKRLDSSK
                     LKALLKDEYADYLKETTSRRLVIKEEK"
     gene            complement(14727..14912)
                     /locus_tag="K8M77_001086"
     CDS             complement(14727..14912)
                     /locus_tag="K8M77_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085595.1"
                     /translation="MGNIPDCCYEYRFNREELRAIKTCTECGYDIYAGEKYLPIGEKI
                     ICSECLDELMEEAEEEF"
     gene            complement(14915..15160)
                     /locus_tag="K8M77_001087"
     CDS             complement(14915..15160)
                     /locus_tag="K8M77_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085596.1"
                     /translation="MDLIRVAKNIADRVQERLSNLTVDKPLNVLIEEEIKRECIGTDQ
                     SNQCTQETIYKDIISPKYDLDNGVIYDIETGETVKEL"
     gene            complement(15255..15437)
                     /locus_tag="K8M77_001088"
     CDS             complement(15255..15437)
                     /locus_tag="K8M77_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085597.1"
                     /translation="MNYKYIESLKARYSKEEFENILLSVEQDLKFNNLRFKNPISEKY
                     FLEVLNITEKLYRRII"
     gene            complement(15447..15626)
                     /locus_tag="K8M77_001089"
     CDS             complement(15447..15626)
                     /locus_tag="K8M77_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085598.1"
                     /translation="MLTPGGKLILGIITIVVSLYLSIDFMLKSLDEKKAKESFKNILL
                     SVANMLILIFATNVI"
     gene            complement(15620..15805)
                     /locus_tag="K8M77_001090"
     CDS             complement(15620..15805)
                     /locus_tag="K8M77_001090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085599.1"
                     /translation="MAKKHDKPKVTWVDGKPFITNGWIDGSYFEIKQQPSIRSIEYVN
                     EVFAENAHKCIKEKKIC"
     gene            complement(15931..16656)
                     /locus_tag="K8M77_001091"
     CDS             complement(15931..16656)
                     /locus_tag="K8M77_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HBI7085600.1"
                     /translation="MNNLMIFKNEDLGIDVRTIKNEDGSISINAEDAALGFGWYQIKN
                     NKRYVKWERVNGFISEFENSPQVEKDDYIPESLFYLLAMKANNDVARKFQTWLAVEVI
                     PVIRKSGQYQLPVKKNLSSIDLFEAQVKALREVEEKVNEVDKKFDDLPLFEVDSKDLK
                     KKANRVVVALLGGKKSNAYKPLSKKVFSDLYGQIHREFGVNTCAAIKRKDLDLAKEII
                     DNYTLPRALKEEIELANSQLAFA"
     gene            complement(16720..16905)
                     /locus_tag="K8M77_001092"
     CDS             complement(16720..16905)
                     /locus_tag="K8M77_001092"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085601.1"
                     /translation="MTKLKQKRMELGYKQYKIAERLGISTRHYARAENGERLATDKEL
                     DVLSKIFKCTLNELKEE"
     gene            17124..17498
                     /locus_tag="K8M77_001093"
     CDS             17124..17498
                     /locus_tag="K8M77_001093"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085602.1"
                     /translation="MDFKDKLKDFRKRNSLTQAEFAKKYGFSRTTITELESGRKKPTL
                     KMIDKIAKATNTKTVEWLDEKENIEIKNFEGLKMVFDKLQETGDIDEEGNMNQKAKTF
                     IFKMVEAEIKLLAQQKKNKEKN"
BASE COUNT         5223 a         3451 c         1787 g         7191 t
ORIGIN      
        1 taagtctatt tttttatttt actaattatt tttcttatgt acttattttt ttggttaaac
       61 atcttcatct tcttatctct ttcagacatt atattttttt cttttatata tctaggagat
      121 gaaatgtcgt taattccgtg actgacataa taattatatt taatatctgt ttttacattt
      181 gtttcttcat caatataata actagaactc caacaacaat ctactactat atcttgctca
      241 tagtaatttt caatcaaatc agtatacgct aatttaattt ctacaggtac tctaccgcac
      301 ccatctctat ttatactaaa tttgccttgg taattgacta aagctctaaa acttaaatgt
      361 attttttcac caactttcat aattttataa tcaatatatg aataatccgt aattccacag
      421 tattttattt ctaaaatact taaattcaca gctgaattca tcccaacatt ttcaatatct
      481 atataaaaaa acaaatcata ttcatatgga acaccaggct ttattacttc atctactatt
      541 tttatattgg gctgtaaaat tatatatctc cataattttg actttggttc ctcttgaatt
      601 atattatatt gaagatatgg tattacttga agtcttctat cttcattcct cgcaattgtt
      661 tctgatttta tagtcttcct aagagcaact aatgtaacaa ccccacctaa aatagaacct
      721 attatcgatc cccaaaaccc tatccattct ccttctatac ttttccaatt aattaataaa
      781 attataattg ctgaaattat actaattgaa actataacta tcattattaa ctttaatatc
      841 ttctttccca tagctcctcc atttataatt aatgatataa aaaattatat cattaattat
      901 aaattaaagc atattctcta tactgatttc ttctaagctc tttattgctt tccaaatgat
      961 cctttaaaac tttttctaat tctttaacct ttttacttgc tatttcttta ttctcttcat
     1021 cacaattacc agccttaaat ctcttccact ttcttaattg tctttcataa tatctctgtt
     1081 tctgttctgc ttcataaagt ttaatggctt ctttaccatc tggtatagtt ggtattctag
     1141 ttatacctgg aaagtaagtt gctaatgtat gcctacagtt aggatgtaag aatccttttc
     1201 ctacagcctc acttaataat ggataatctc catcctcttt agtaccatga gaaaatatat
     1261 catcaattaa tacttttcct tgccatggtt cacacatttt acaagtatta gcatgagccg
     1321 taacaactat taaatgtatt ccatattcat ctcttttctt tccttcacct aaaaatgtag
     1381 ctctttgact tgctgttctt aaacacatct ctgcataact agcaatatta acttgtttac
     1441 cattcttata agttatacta tttatacctt tctcaagaaa atccttagta gccatatcta
     1501 tagcttgatt aatagtctta gtaccacttt gcaaatatac atgagtttta aatattattt
     1561 gcctatatac atcatccatc tttcttaaaa ctgacatttg agcctttttt aaatcatttg
     1621 taacagtttc ttgtaatgca ttaagtttct tttcgctaat accaaagaag ttttcttcaa
     1681 cttgtggagt agcctttttc cctagttctt tagcaatata ctctctaact gtttgaggtt
     1741 cttttatatt ctctggaaac tttatcttta cttcatctat taacttttct gtattttctt
     1801 gacctttagt aaaattccct tgaatctctc tgtttatagc ttcttggata ggcttgttat
     1861 attcctcaac tagtttctta tttctctttc tatacttttc aatttctcta agcttagtcc
     1921 tttgccattg ttcccattga aatccttctt tactctgctc ggcttggtga aaataaaaag
     1981 ccctatgcat actagaaatt aaatcaagct ccatttgctc gaatatattt ctaatatcat
     2041 aagacttatc tctttccttt ttagcatttt cttttatttt attttcagta attcctctaa
     2101 gtatatcagc taataattgt atatccttat ccctcttgtc catctgtttc aacctcatta
     2161 tcatcagtat atgacatatc ataatcatct actgtcttag gttcttcagc cacaagatag
     2221 ccattctgtt tctttattct ttgtatctct tcttcctttt cttcatctgt ccatgtatct
     2281 ccatacattt cctcaataca ttgctctata gacattactc cataggtttt agcttttcct
     2341 acagtttcta ctactgtatc aaagctagga cttgcatatt ctccaaatac aatacttact
     2401 tcatattctc cagtattctt tttatttaat acatcattgg tctttaagat tatattaact
     2461 aactctggta taacctctgt taatatatca accatttttc ctctagtata taaagtagtc
     2521 ttttctttct ccctttgagc ttctgcatta tctgttttct taaggtctat tcctaaagta
     2581 ctagggctta ttataccttg taaacacata tcaatagcat ttgaataact ctcaacataa
     2641 gcctcataat ttatattagc ttgtttcata tctatctcat tttttgcatc ttctgcaagg
     2701 ctagaaccta cttttaagaa tctattatca aatggattag gtttcattaa gttaccatta
     2761 atatcttttg gaactaagtc ctctggtata tacttttgaa ctcttccatc ccttatggca
     2821 tctatccatt gacttataac ttcatctaat gcatcaaagg tatctgattt attatcaaat
     2881 atactcttac ctcttccctc gaacttagga gatttaaaga acattaatgg tactcccatt
     2941 ataaaatcat ctttatatgt tacatctgca agttctctag tttcataaag tgtatttaaa
     3001 gaaacctcat taccattgct atcatataaa ttataattaa tatatccttt tccataagtt
     3061 tcacttaatt tatattgtct attattttta gtgtaaaaag tataaaactt tatttctttt
     3121 aatcttcctc tttgagttat atattcaacc ttatcaccat cgaaaaactc tattattgga
     3181 tatttgctta tctctgtatc tatagatagt ttaaaagctc catcaccact aactaatgta
     3241 gtagcaatta tatcacctag catattatta aacttattat cttttcttat ttcttcccat
     3301 aatgtattat aattctctcc tgtaacctct atactatcta agtcagctac aactatatca
     3361 cttaacttat cagctatcat tgcaggtaat ccactatgta tttttcttat acttaaatct
     3421 tcacttggaa cagcactcca aaatctagct ttatttactg gatcacttga tatgttctta
     3481 aagaattggt ccagttcata tggctctccc ctataccaaa gcttatttct aattacatta
     3541 gtttcaaatg tataagcttc ttgtatagta ataggattag ttaatgctgg ttgaacattt
     3601 aaatatttaa ttgctgcttt agttaacata ctcttaaacc accccatttc tattcctcct
     3661 tataatctcc tatcaatttt ctaaaaggta tccatgcgta ctgactagag ttgatcgtat
     3721 ggtcgtttgc atcctctggc tcatatttat cctctttcca gctatagcac tctaactctc
     3781 ttatatgctc tttacaagta tctacaacat aataaaatac tttatcatta gtattaatcc
     3841 aacctagcat taaatgtatt ctgtctaata tggttacttt cttataagag ttaataaagt
     3901 tatacataca tggattagtt cttttaaact tcttaagctc cattatagtt gcttggtctg
     3961 ctgaatctac aaatacatct ctagcaaatc cccattcttt tctattcttc tctaagaact
     4021 taaaatattt aggagctata tcactaggag ctaatggagt ttctaagtct ttattgttat
     4081 atacctcttc atctagtatt actaactctt tcttatctgt aatacctaag aaagtaaatg
     4141 caaaggtatc aggactattt tgagaatatg ctgtatcaag ccctgctgta aattgaacaa
     4201 atttcattcc cttagctttt tctttagaaa taacattatt ctttctttca aagttactga
     4261 atattaaacc tgttgctctt cctctcaaac ctagtatctt attcttatat agcttagtgc
     4321 ctttaggggc actcgtcttt ttcttttcaa tagcttcctc acttaatgat gcattatcat
     4381 agaaagtaaa aaaccagtag gtccaattag gtttttcttc tgaattgagt tgccctaata
     4441 tttctactgg tacatctttc ttatattttt ctaatggtct acaacaatta ataaactctg
     4501 aatatatagg taagttagga tcatctggat taagtgtcat catgagataa tcatttctag
     4561 tacatatctc tctaacaaat tcaatactag ctgtattaac ctcatctata agtacacaac
     4621 cgaattgaga acctaaggcc attttccact tatctacgtt atcataacct agtatatata
     4681 ttattttctc accattagaa gttatatatc ttatatgagg tattttatta tctttatcac
     4741 cattaccgtt atacttgact aaatcaccaa atacatcagt aattccatat tccttttgta
     4801 ttaagttctt ttcagcaaca ccagttgttt tagcagcaat aacatgcatt tttttaggag
     4861 attctgcaac cattagcata aactttaata ttcctactgt agtttttcct gctgcggttg
     4921 ttccctccaa tgcttctact ggtgctctgt gctttaaaaa agctaaatac ttctctgata
     4981 acttatattc atcactcatc gtcatcatcc tttaactggt taagtataga atctagttta
     5041 gttgtagaat taacatttac gttactatcc ttttcaactt tatcaacaaa caatctatat
     5101 ctcttaccta ataactctgc tgctttgatt ttatctttag ctgaaatttc cttatcaaca
     5161 actttagctt cactcataaa atcacctata ttttcagtaa ccactacttg ctctgtttct
     5221 tctcctctta gaactctagt taaatactct agcacttcct ctgctttagc tatcctttta
     5281 ctttctaatt ctttcatctt ttcatctata taagatttta aggcaggttt tagcaagttc
     5341 tcttgtccta ttaccctggc tgtctttcta ctatatcctg ccttaatagc tgattctgtg
     5401 gcgttgcctg tttcaatata atactcagcg aacgcctttt gttttggtgt aagcttcata
     5461 acaacaccac ctttcttttt attttattta cttccatcaa aaatggtagc aatagaaagt
     5521 gcctctattg ctaccatttc aactatatat cttacactaa tattctaagt ttattgtttc
     5581 atcaagcatt ctgctggatg tggtgttata tcttcctcaa acttagtgtg gtatattatt
     5641 tcattgactt cttttttaat aacattaata atgcttgtat ccgattctaa tgcttctttt
     5701 atcattttaa tagttatatt tattataaaa gtatcatctc ttaaatataa ctttttagtt
     5761 aatccaactc catcttccca aggtgattga cctgttattt ctttatttgt aaccataaag
     5821 ccaaatttaa cctttttata tctcattaac gagtaaaact tacctattaa cgtaacatct
     5881 atagactttt tatgattttt acattctact aaacagtctt tccttaaata ttctggtaat
     5941 aacttttcat aaattgcatt attggttgct ggttgtaata acaaatctat ttcattgcta
     6001 tcacacacaa ctctttcttt aacagaaaaa atatttctta tatttaaaac tttctttgct
     6061 aatttttcta ataattctcc tttttcaaaa gacaatttac ctttctctgg tctagtacta
     6121 gaaggcatta tcctatctat ttcctcaatt ctataaatta tttcaaatgc ctcttttttt
     6181 gttgtatcag gagttttgaa aaataaatca attccataat tcggattttg ctttgcaagg
     6241 ctatctataa gatctgcaaa cattaaaatt tcttcttctt tcatactaaa cacatacctc
     6301 aaattcaact actaaattct ttattaatga acaaccctta tcacatctat cacaaaaact
     6361 aactggaact tgtgctggat catcatatat tttaacagca ccagtaacca tgtcattttc
     6421 acaatatact ttatattttg gtttcaacat gtcttttgtc attagataaa ccattaattc
     6481 atgagtttct ttatatgaaa tttttaaaat tcttttaacc actgatgctg gtaaaatccc
     6541 gtgattatga aattctaata cttctgataa atctttaatt tgcttttcag ttaactctgg
     6601 attataattc tccaatatct tttgcaattt ttccaataaa tctaataaca tgactaattc
     6661 tctccatatc atttatttca ttatcatata tgtttaataa tgaatattca ttcttattat
     6721 agttaaatat aatttttaaa tctatagtct tctctagtgt aatagtattc cctttttcta
     6781 tatattttaa ccaactaagt attctaaact tctcattagc aaattgttta gtttgattca
     6841 tataattttc tatttcttct agacattttg tcacatcatc gcaattatat ttttttctaa
     6901 gctcgtttac taaaacttct aaatctccta agaaaggcat tactccatct tctccaactc
     6961 ttaaggttaa accttttgac ttagctgttt caaatgacca aattatctct tcaacaaatg
     7021 ccttatgatt atttacaatc tctcttattg tattttccat atcaaaatat tcatatttaa
     7081 attcataaat agattgtaat tgtgctagtc tagcttctat tgttctatga tgaaaagagt
     7141 agtcgctatt gaatgataat ctatcgaatc taacttcaaa taaattaata tttttatgaa
     7201 aaacaaatac tattggatac cttaaacatt tcatactaac agtcttttta tcaagctcat
     7261 ttagatattt atgaaattta ataaagaaat aatcatcatt ctctaaatat tgaaactcct
     7321 cattattgat agagttacta taattattgt agtattcact atccagatta gttgctactt
     7381 ccataagttt atctcgtagt tcatcaaaat ttatattatc aaatttataa actattgaat
     7441 atctatatcc ttttttaatt tctaataaat cgaataattt tagtgttaag ttgtttttat
     7501 ccaaacatgt atttattctt tcattatgat ctgccattcc tgttaataat acaacacctt
     7561 catgttcttt cgtcccatat ttaccaacat tattactttt tatatcatta ataagtttct
     7621 gtttcaggtg atttgggaaa tctaaaaaca ctgtgtcttt tattatttgc ttatactctt
     7681 ttaaatccat tgatgcctcc gttatttctt gcacttcaaa aacaaatcaa gtataataga
     7741 tatatattaa tgaaatacaa tttattttat agaaaccact tttgatttaa cccttatagt
     7801 ggtttctttt attttataat taattatata gcttgtctat gcaaaaaatg gaaaatccac
     7861 tcttaaaact ataaatttta ctatatttac aattatctac agttttgatt tgttgtaatt
     7921 tcataaagaa aataaaaaga acctagccta aactagattc tttaagacac atcacgcttt
     7981 atcattataa tccctgctat aataaaacgc ccccaaagga attttattta atttttagtt
     8041 atggaaagta agagattcga actcttgata aactattcag tttgttatga ctgccgtcat
     8101 tcccttaatt cacttgggta actttccata tagcaggaac ttaatcctgc aatgtaaggg
     8161 aaataagatt tttaaataag aacttttaaa ttagcaattg tgattaaaaa tttgtaaaag
     8221 aggaaggtaa aggagttgca cctttattaa tactcttcct tccacgatgc aaggctttta
     8281 caccttgctt tccgttagtt aagttttaat ttacatctag gaggatcata atgaacgctt
     8341 taccctatcc acaattacat aataacatat aaaagtgggt atatggtggg tattaaacgg
     8401 gtataaatag gggggtatat ttatataata aacctttctt gaactcctgg attatagaat
     8461 acaaaatctg caatttgctt tataacctta ggtcttaatt ccttcctgca ataatcttta
     8521 ttatatttta aatgtctagc aactgttccc caactaactt taggttgatt tatatatctt
     8581 aatctaatca aatccttctc ttcttttgtt ctaagagttt gtatagctaa atctattctt
     8641 ctctttaatc taactttata atctaaatct ctcttaagat agaataaatc tttttctttc
     8701 cttattactt catcttcaac catgctgcta aacttatttg ttgggccact cttttcatta
     8761 taacctatag ctccacaacc agtataagat tcttgaatat ctttaatttc tgcttctatt
     8821 agttttattt ctgtttctaa tgtattgtaa tttctaagta aattttctgt ctttttaaat
     8881 aatgtcttgt ccatagttcc tccttaaata ttaaatccta tctgtcctgg taatatttct
     8941 attatttcat cttcttcctt atcagctttt tttaatagct cttgccaatc atcacaatct
     9001 aaagattcat tttcatatct ttcactaagc ttttcattaa gatttatatt agttatatca
     9061 aaaagctcca catcactatc aatatcaaca gtttcgttat ttgctaattc agatacaact
     9121 tcccacatct cttcttcatc tagtcctcta tcaactaaag tagcaacttg tattgtaatc
     9181 gtaacttccc ccctaactaa tttcatttct ctcattactc ttcaccttcc aataactctg
     9241 gattctcata gatgttacct atcagttcta aatctgtata attaaatttt aatgccatac
     9301 ctttgaataa tggttttaag ccaaaaccta gtgctctata tacaattaaa tacttttgat
     9361 tctctatccc taaatttaat tttgatttat tagtgttgat taaaatatct ccctcataaa
     9421 tctcttttcc gttcttatcc ttcagtcctg tatattgcaa tatttctgct tctataccat
     9481 cttcataatc taatacataa tgtgatttat cgctctcttc ctcagctatt aaagtaacca
     9541 ttttattttt agtatccaac tcaactcttt caaaatcatc ttgatataac atactatctg
     9601 atgttttatc ccacactcta aacttaagct ctctactcat gcttattcct ccttataaat
     9661 atctcttaac atattcaaag ttcttagtta ctctgcattt atctctatct ttcttatcaa
     9721 aaaaatgtat tctatacact tcaccatact tgacaatctc gtattcttct gtttgtttga
     9781 ttaccctgtt ttgaagtata tcaaatgcac taatttgtcc ttctaacact tccatttttc
     9841 acaccaactt actcatttat ctcaatttct tctaaaatta tagcttgatt tttaatatcg
     9901 ctatcaccat attcataact cggctcttcc caatgataaa catccgcctt taatctttct
     9961 ttatccttgt acattattat tggatttttt ctaaatacta tttcaatatc ttcatgccat
    10021 tttaattgat ctctaaagct aagaacttct tctccgaaat ctactttatc gcaccaatca
    10081 tctttagtat ctctcattaa ctttcttaac ttattattga atattcttaa tgcagtatca
    10141 taagataatg catattcaat acaatattca atccacatct cttgtaaata caccctataa
    10201 gctttcatat taacacctct ccgtatcttt cttataaact ccatagaacg ttttatctat
    10261 taagacaata taactatcct ctttctccat aaaaacctca tgaacgctat ttttagttaa
    10321 tagaggatgg agccttgtcc aaccctccaa caactttatt tttggtttat caaagaaact
    10381 tatttgatcg ctcattttaa taaagtccta acatactttg agtttttttc atatcacctt
    10441 caaactcaaa gtaacaatat ctactactct ctttagtcgg tttagctcct ataaacttta
    10501 actgttctat gagtaactta tttctcttaa cgcttacttt aagttcagtt ctattgctag
    10561 aaatcaatct taattctata ctcacttttc tacacctcac ttaattcaaa ttcaaccctc
    10621 tcgagttctt ccgtatattc ttttataact ctgaaatcag ctacgtaagc atcatcttta
    10681 taagcaatac cattcaagct atctaatata atctttccta tgttatccac atctggtttc
    10741 tttcttggct tttctaatct gtcccttata gcctgtacac gctttttact ataagactta
    10801 ggtattttat agtaagcaac aattaaaacc tttatagagc cttctaagta tcttccatct
    10861 tgctcctggt aacaatctct tactaacttc tcatactcaa ctgtatcttt aggagtaaag
    10921 gcatgacctc tacacactct aggtctagcc tttcctctta tctttcctgt aactactacc
    10981 ttcataaact acctatcctt tgaatttatt actctttcag catatctaaa ctgctgcttt
    11041 atataatcat cttcatatga tccaccactc actatccaat cacctattct gtattgaata
    11101 tctaaagcaa cctcttctgg aagtttatct gagtgctttg ttaattctct tattgaattt
    11161 atctttacca tctgtatcct ccaaccttta cgctccatct ttttctaagt ccaatatctc
    11221 tacacttaag ataagcgtaa acttctttat ctttcttttc tctcttagct actatatctt
    11281 tataagtcat aattccaagt tcatgcggtg tatacatatc aagtaaattg accttcacta
    11341 aaaccacctt ctttatctaa aatttttcat tctataattg aactcttctc catggaatat
    11401 aactatattt ttatcaactc tttccataat cctacctgct aatgctccat ctagttgttc
    11461 taacatttct ggagtacatt ctgtagaaat aatggtagtt aagtcattat aatatctata
    11521 atttaataaa ggatatatgt gtttcatatc tatttccgtc atatcagcta ttacatttcc
    11581 atttctaact ttgtctttga atagatcatc aatcactagg acctctgcat ttttatattt
    11641 ttctaataat gcagtatatt tttcaaagtc tttagcatta cctttcaagc ccataatagc
    11701 ttccgtataa ggcatatata taaccttctt atgtttttct ttaaataaag ctactccaat
    11761 tcctatagct aaatgacttt tacctgctcc aggttgtccc attaaaagaa accaattatt
    11821 ttcttgctta ttttcaaaat ttaaaatata atctacagat tttttcttag cattttcggt
    11881 taatttatca taaggtttat attctgatat tcttttagct tggctaggct taaccccaaa
    11941 ctcactccaa gctctttcta tatctctatt gaccttacaa gaacattcct tagcaagtgg
    12001 ttgtaaatgc tctctatact ctaaaataaa acctgtatcc ttacataaag gacatttata
    12061 ttccattgat cttgttgtag tcatattttt gctctgttgt cttgcctttg ctattatcct
    12121 gtctaaagct tccatcatta actcctccat tagttttact atagttaccc tctaatactt
    12181 taggaaagtt gttaggctta ataaaccaat caaatgttat ttcaaagtct ttaaccttac
    12241 ccattagaaa atcacttttt ggtatttcat tgatagcatt aaaaactacc tcttctgaat
    12301 attgtttaat tctagctttt aacattttat atctattagt acctggagtt attgttttta
    12361 aggtagtaga taatcctaaa gagttccact tctctattaa tcgttgcact gtagtgctac
    12421 ataatatatc tttagatata ttattatcta tactatcctt acctaaccta accttacctg
    12481 ggtttccatt tggtatgtca cttggtatac cattggtatc catttggtat ccatttggta
    12541 taccaacatt attttcacta gaatctgtat ctattaaagg cacactattg cttaattcat
    12601 acatattatt ttcttctaat aacagttgtc ctttttcctc ttggtacatt gtaggcttat
    12661 atctatcgtt tctaatataa ttgtgtattc tccaatgctt tataacacat ataccactct
    12721 caaatggtat tatgaattgt ttagctatta gtaactttat atcatcacca ccacagccta
    12781 ccattctttg tatctttctt ggattattta cgaatccatc atcatctgct ctcatactta
    12841 agtgaaagta tagagcttgg gtggatagtg gcatatctaa aaatatgtca ctatctatta
    12901 ttgtttttga aaacattctt ctttctgcca aactcctcag ctcctattct tctaatggtg
    12961 ttccttcaaa catattttct tgcccttcaa gaatttctcc attactatca actgtataat
    13021 ctacatcaac aacaccgtca ttttcttcct gtatatcctc aatgttagct tgtacatttt
    13081 caacatcatc cataaactct ttatttatta agccttcatc tgctgtatat gccttttgca
    13141 tttctatact taaaatcccc catttagata ataggtttct taaaactgtt ttcttagcca
    13201 ttgcatcaaa attgcttttc caaactgaat tttttgagtt tattgtcttt gaaaatttat
    13261 ttgcatgatt attaacttct tctttagtcc agaatactgt cttttcaaat ccatttatta
    13321 atttgaaata tcctgcatac ccgattactg catcactctc acgcttacta aagtcaactt
    13381 caatttcttc tgttaatgga ttccatgaaa ctaattcgcc ttcccttatt tcaactacat
    13441 ttatagcctt atattgacct gttcttaatg ctaattgaat atatccttta taacccattt
    13501 gaaattgagc tctattgcca taaggaacta cccatgcata tcctaaattc ttgtccactg
    13561 gtaaatccat tgttgctgct accatacaac tcgctattac actcattcca tcacactttt
    13621 gtaagttggt atctgaatta actaaattaa ctattgaact catatattga ggagccttct
    13681 cttttaatac tccttcaaat ctcttcttta tacttggatt tttcattaat cccttaactg
    13741 tattacctat tgataaactg ttaccagttc ctttctttgc taattgattc tttaaacttg
    13801 atgctgttgc cataattatt tttcctcctt aatcactaat cttctacttg tagtttcttt
    13861 taaatagtca gcgtattcat ctttaagtaa tgctttaagt tttgaactat ctaatctttt
    13921 ttgacttact gatttatagc taatactata tccaggagca ttagcatact cagcattttt
    13981 tatattttgt ttaagctgat tctctatctc tttaatttgc ttgtctacat catctctata
    14041 ttctttaagc tcttgtcttt gattaagtaa ctctctccaa gatgaatcta aattgatagt
    14101 ttcaccttca ttaacattct tatatttttc attaacccac ttttctgcag cacttgatcc
    14161 gtctaatgca ggtggtgttt tatcctggac catcttccaa aattcttttt ctgcctctat
    14221 gatgatatta attaattctt catcacgatt aatctctttc caaataaact tctggccacc
    14281 tatcaaaact gctatatatc ctttagatgc tcctgtaact gctaagtagt gttgaacttg
    14341 taataaatag ctaggtggta cttcttcact ttcccattct ttagctaagt attgattagc
    14401 tgttttacat tctaaaattg catcttcacc aacaactctt ctatcaatat tagcaaccat
    14461 aaatggataa tctttatttt gaaagtgcct tctatctctt cttacttttt taccagttct
    14521 tctttcaaat tctttagcaa ctacttcttc aaattgatct ccccaataag ctgcttctga
    14581 ttgcttttcc aactttataa taggttctgt tttttcaaga taaatattaa aagctgtttt
    14641 atattgacta actcccatta tggctcctac atcggagcca cctattcctt tctgcctttc
    14701 tctaagccat tctaatttat cttccattag aattcctcct ccgcttcttc cataagttca
    14761 tctaagcact cactacatat tatcttctct ccaataggaa gatacttttc acctgcatat
    14821 atgtcgtaac cacattcagt acaagttttt atagctctta gttcttctct attgaatcta
    14881 tactcataac aacaatctgg tatatttccc ataattacag ctcctttact gtttcaccag
    14941 tttcaatgtc ataaattaca ccattgtcta aatcgtattt tggtgatatt atgtccttgt
    15001 aaattgtttc ttgagtacat tggttacttt ggtcggtgcc aatgcactct ctttttattt
    15061 cttcctctat taaaacgttt agaggtttgt ctactgttaa gttactaagt ctttcttgta
    15121 ctctatcagc aatattctta gctactctta ttaaatccat tactaacaca atatcctcca
    15181 tgatatatag aagaaaaacg tccaaataat taatgctaat aatgtaactc ctaagcaccc
    15241 tatattaatc tttttcatat aatcctccta tataattttt ctgttatgtt aagtacttct
    15301 aaaaaatact tttctgaaat aggattcttg aatctaagat tattaaattt taggtcctgc
    15361 tctacagata ataaaatatt ttcaaattcc tctttactat atctagcttt taaagattct
    15421 atatatttgt aattcatctt acactcctaa attacatttg tcgcaaaaat tagaattaac
    15481 atatttgcaa cacttaataa tatattctta aagctttctt tagctttctt ctcatctaag
    15541 ctcttaagca taaagtctat gcttagatat aagctcacta ctatagttat tatccctaag
    15601 attaactttc ctcctggagt taacatatct ttttctcctt tatgcattta tgggcatttt
    15661 ctgcaaaaac ttcatttaca tactctatac ttcttattga tggttgttgc ttaatttcaa
    15721 agtaagaacc atcaatccac ccattagtta taaatggttt tccatctacc caagttactt
    15781 taggtttatc gtgcttttta gccattccta ttctcctttg ctttatttac ctttagctct
    15841 aaaaagattt taatagagct tgtcttattc attcccttag tatcagaggg atatggtatt
    15901 tactgatact tgaccaataa atccatagcc ttaagcaaat gctaattgac tattagcaag
    15961 ttctatctct tcttttaatg cccttggtag tgtatagtta tcaattatct ctttagctaa
    16021 atctaaatcc ttccttttga tagctgcaca agtattaacc ccaaactctc tatggatttg
    16081 tccatataaa tctgaaaata ccttcttact taatggttta tatgcattag attttttccc
    16141 acctagtaat gcaactacta ctctattagc ctttttcttt agatccttgc tatcaacttc
    16201 aaatagtggt aaatcatcaa acttcttatc cacttcatta actttttctt cgacttctct
    16261 taaagcttta acttgagctt caaataaatc tatactggat aaattctttt ttactggcaa
    16321 ctgatattgt ccactttttc ttattacagg aataacttca acggctaacc aagtttgaaa
    16381 ctttctagct acatcattat tagctttcat tgctagtaga taaaacaaac tttcaggaat
    16441 gtaatcatct ttctccactt gtggggaatt ttcaaattca cttataaaac catttactct
    16501 ttcccactta acatatctct tgttattttt tatttgatac catccaaaac ctagtgctgc
    16561 atcttctgcg ttgatagaaa tacttccatc ctcatttttt attgttctta catcaattcc
    16621 taaatcttca tttttgaaga tcattaaatt attcatcagt tgaacctcct tctatttcat
    16681 tcaaaattct aaccagcgta tttaaatact cttccacact tactcctcct tcagttcatt
    16741 taaagtacat ttaaatattt tagataaaac atctaactct ttatcagttg ctaaacgctc
    16801 tccattctct gccctagcat aatgcctagt gcttattcct aaacgttctg caattttgta
    16861 ttgcttgtat ccaagctcca tacgtttttg ttttagtttt gtcataagtt tatttcctcc
    16921 cttccatgcg tttattatgt cttattttta cgacattatc aaatttactt tttcgtcatt
    16981 tttctttatt ttttaaattt aactttcaat tgcttgcatt ttctaaactt ttctttgtct
    17041 tattttagcg acgtttgtat tcttttttgt cttgtttttc ggacatgatt atgttatcat
    17101 taaaaatgaa aggaggaata catatggact ttaaagataa gttaaaagac tttagaaaaa
    17161 gaaattcttt aacacaagct gaatttgcta aaaagtacgg attctctaga acaactatta
    17221 cagagttaga atcaggaaga aagaaaccta cactaaaaat gattgataag atcgctaaag
    17281 ctactaacac taaaactgta gagtggctag acgaaaaaga gaatatagaa attaaaaatt
    17341 tcgaaggttt aaagatggta tttgataagt tgcaagaaac tggggatata gatgaggaag
    17401 ggaatatgaa ccaaaaagct aaaactttta tctttaaaat ggtggaagct gaaataaaat
    17461 tattggcaca gcaaaaaaag aataaagaaa agaactagag gttaatcctc tagctctttt
    17521 aatatgcttt ttaaagtttt taaataatct tccaccaaac tccactccca attgttctta
    17581 tttatacgaa cgtacgttct tatgttttat gattttattt tactacgttt atatttttat
    17641 ggcaactgtg ga
//
LOCUS       DAEVGI010000036        17548 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEVGI010000036 DAEVGI010000000
VERSION     DAEVGI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.036"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            116..1516
                     /locus_tag="K8M77_001094"
     CDS             116..1516
                     /locus_tag="K8M77_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI7085603.1"
                     /translation="MEKYIFWVTAIFQIGVFALMLYYLFIALFGFYKKKDTKTFEPQK
                     KFAMLVAAHNEEVVIENLVESLKHLDYPKELYDIFVIADNCTDDTAKIARAAGANVFE
                     RFNKEKRGKGFALEWMFDKIFKMDTHYDGIAVFDADNLVDKNFLKEMNNKMCEGFKVV
                     QGYIDSKNPQDSWITESYAIAFTSANRMLQLAKSNIGLSAQIGGTGFVIDTATLKKLG
                     WGATCLTEDLEFSCKLILNGEKVGWAHDAIIYDEKPLTLKASWNQRKRWMQGFADVAT
                     RYGMKLLKKSIKDRSLVAFDCTLYVMQPFVTLATGLAGLLTFVQIRAVDGLGIFVISY
                     LFNATVWQLFCVFQFLLTPFALLFDKKMSGKLFVVLGLYACNVFIQPYLINQLGILDN
                     IMLLTLFNVAYIGIFLIGTLLFLGKRNFQIFYRYLIYPLYILTWIPITIQGIIDKDKK
                     EWSHTKHVRQIGIYEV"
     gene            1762..2832
                     /gene="mnmH"
                     /locus_tag="K8M77_001095"
     CDS             1762..2832
                     /gene="mnmH"
                     /locus_tag="K8M77_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBI7085604.1"
                     /translation="MFGLISYEEIYGHEDEYIFIDVRSQKEAFEEPMIGSVNIPVLLN
                     EERDVVGTLYVRESAEAAKEQGIEFISRRLPEIFKQVQELYRNKHGKKLVIFCARGGM
                     RSGSLHSLLNSLGINCYKLEGGYKSYRSFIINKIEEFSKEIDFIVLYGNTGVGKTEML
                     YKLEEKGYDVLDLEGCANHRGSILGGVGLGDCNTQKRFDALVYDKLRHRNSNIVFIEG
                     ESKRIGRILIPDPLFEKMYEGAKLKISADPEVRAERLVKEYTAFENVNEQLDEALGVL
                     KKHISPERVEQYKDLVAKGDYKKVALELMEKYYDPRYGTSAKKKTFRNELEINDIENE
                     LFKLEEIYKEISEELREDVYEQ"
     gene            2822..4768
                     /locus_tag="K8M77_001096"
     CDS             2822..4768
                     /locus_tag="K8M77_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIA DNA topoisomerase subunit B"
                     /protein_id="HBI7085605.1"
                     /translation="MSNKNNNYDVTSLTSLEKLEPVRVRPGMYIGSTGSKGLHHCIWE
                     ILDNSIDEISNGYGNKAKVILNKDKSVTVIDNGRGIPTGMHPIKKKTGVEMVFTELHT
                     GGKFNNQNYKTSGGLHGVGAAVVNALSRWLEVEVKQNGKIYRQRFEYAYDKELKKDMP
                     GTPVTPLEVVGESKETGTKVTFLPDREVFSTSDFKFDIIDERLQELAFQNKGIRLELI
                     DNRKEESVSKEYYSERGLLDFIDYLNESKTPLHPTPVLFEGEKEVNNIQMYGEICLQF
                     TDSTTENIVSYVNNIPTTEAGTHETGFKTGMTRAFKEWTKKLNLIKEKDKEFEGDDLR
                     EGMTAIVRIKITNPVFEGQTKTKLGNNEAYTMMNDLAYTKFGHWIEDNKEVATMIINN
                     ALDAAARREKIKKINDAERKKIGKGTAPLAGKVAVCTLKNKEANEFIVVEGDSAGGSA
                     KQARDRRFQTIMPSKGKIMNTEKQKLENVIASEELKIFNTAIGTGILDNYNEEDLKYD
                     KIIIMSDADVDGYHIRTLWMTYIYRYMRPLIANGHLYLAQPPLYKVAKSGKGKDKILY
                     AYSDDELEEVKKKVGKGATIQRFKGLGEMNPDQLWETTLNPETRTLVQVTIEDAAKAE
                     KMVSLLMGDVVQPRKNYMYKYAEF"
     gene            4782..7721
                     /locus_tag="K8M77_001097"
     CDS             4782..7721
                     /locus_tag="K8M77_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBI7085606.1"
                     /translation="MAKKNIEIPKDNNIIRMPLEEVMPDNYLPYAVEVAKDRALPDVR
                     DGLKPVHRRILYGAYMLKAFPDKPYYKSARIVGDILGKYHPHGDSSVYDAMVILAQNF
                     STRAPLIDGHGNWGSIDGDGAAAMRYTEARLSSISMEMLRDIEKNVVDMVPNYSDSEM
                     EPKVLPARYPNLLVNGTFGIAVGLSTNIPPHNLREVIDGTLAYIDNNEITTRELMNYI
                     KGPDLPTGGVLIGEKTLLSAYETGEGKVTLRAKAKIETLENGRLGIVITEFPYRRNKA
                     RILQTISDMTGDKRHAKALDGIVDIRDESDRTGIRAVIEFKKAVDHDMADKVLKYLYK
                     KTDLQGNISFNMVALADGKPETMGLKTIISHYVNHQKDVVTRRTKRELEVAEKRFHIV
                     EGFIKAIGIMDEVIATIRDSKSKKDAHENLVLKFGFTDLQAEAILELMLYRLTGLEIK
                     VFQKEHKELSKKIKALRKILENESVLLGVIKDELKEVAEVYGDERRTALIEDESEAKI
                     DLEELIVAEDVMVTLSNEGFIKKIPLKTYNRSNIDENEIEYREGDYLKFLIKSNTKDT
                     LAIFTDKGTVYQIKCNSVADKKWKDKGERLEDLIRGLSLEDEKIIALESIENFLPNKC
                     FKFITANGLIKKTTLDKFVTAYSKLMAIKLKNDDLLASVSLIDSQDEERFVEIETTNG
                     LNFVVSEPELEFTDRNILGVQLVPLKSGNQIKSIRFVDNYEYKEFIIGINKKGNIKTF
                     SNMNSNSYEKVKVNSFRNIIAFSNKGKVFKFPAYLLQNTEESNISDLVDGFEKDELII
                     KVAPINEFGKIGEDLFVYFFSREGLVKKTSLREFLGEFNNQIAYKFKTPKDELVNVDI
                     NFENATVILVTKNGMGIKFLATAINPMGRIASGVTGISLKDDNKVIFGKVIPPSEGID
                     DKTLEAYNDYKKELTSNYEKLVLESKQKEKAEVNIEDIKLQNRAGRGSSLMILVLEDY
                     IRDVIIK"
     gene            7733..8308
                     /locus_tag="K8M77_001098"
     CDS             7733..8308
                     /locus_tag="K8M77_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBI7085607.1"
                     /translation="MKKVLVFLAEGFETIEALSVVDVCNRAKVTCHACSLTENRTVNS
                     AHGTMVLCDKLISDNDLETYDAIVLPGGMPGSTNLRDNEKVQSLIKKYNEENKIVAAI
                     CAAPIALAKAGVIEGKKVTSYPGFKEELGNVNYVEEDTVVVDGNTITSRGPATALVFG
                     LEILKKLGYEKEAEEIREGMLINFFLEKECK"
     gene            8521..9732
                     /locus_tag="K8M77_001099"
     CDS             8521..9732
                     /locus_tag="K8M77_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI7085608.1"
                     /translation="MQKIILNNGVRLLYKFKDIEHTSFCISLESGANVENKEEIGMAH
                     ALEHILFKGNEKLKEDEINEKLDDLFGFNNAMTNFPYVIYYGTTAKEDFEEGFSLYAD
                     IVLNSDLQEFGFSEELNVIKQESDEWKEDLEQHVEDLALMNGLPDERIGNLIIGEKNH
                     IEAISFKGLKDFYEKNYLSENMVVSVVSSLPLEKVKEIVEKNFNRAKRGKISKYSLER
                     NINCGIFSKKIEGNTGAKICCLFDINDLSMEEVTLLKVFNLWFGEGVSSVLYDEIRTK
                     NGLAYEVYSEVKYEKGIRLFKIYLGTSKEKEEEALGLIEKCISKAMDIEDYLSEEGLN
                     KLIKRFKLKNSLDLEKSIVLANRMAIYETMFNRGEYIFEELNLIENLSLKDMKNLIKR
                     VLKKSIVQIIN"
     gene            9744..11522
                     /gene="recJ"
                     /locus_tag="K8M77_001100"
     CDS             9744..11522
                     /gene="recJ"
                     /locus_tag="K8M77_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBI7085609.1"
                     /translation="MREQWLLKQRKRDEIENLKEELNIDLLTSILLVNRNIKDEKDAR
                     EFLFGGIENLNDPYLMKDMEKGVKRVKEAIEKGDKITIYGDYDCDGVSSTSILYKGLK
                     RCNANFNYHIPDREDEGYGMNSKRVKILKEEGTKVILTCDNGIAAFEEIDLAEKLGMD
                     VILTDHHDIPLVKDENGEINKEIPKAYAVINPKQEDCNYPFKSLCGAGIAFKFICALY
                     KELGIPQEEALELLEICAIATVCDVVDLLGENRIIVKNGLEMLNNTKNKGLIALKKYT
                     GLEGREMAQYHLGFVIGPCINATGRLETADLSVELLLAEDDERADALAKELFELNQRR
                     QELTKDSVDMVIKQIEDNNMENDKVILVYNSYIHESIAGIVAGRIKEKYNVPTIIMTK
                     GKDMPKGSARSIEEYNIFEELSKCKELISKFGGHPMAAGLSVEEKNIPLLRKMLLENC
                     KLTDYDIIPKVRIDVKLPLESLNYGLVDSLDKLEPFGKGNASPLFGEKDVKVSRVWIM
                     GKEQNVIKFRCKLRNSYKTIDAIAFGNKVEEFKEDFANKYGEEELLRVLDGGNCNFSI
                     DLIYYPNINEFNGNKSIQANVKYFRL"
     gene            complement(11643..11822)
                     /locus_tag="K8M77_001101"
     CDS             complement(11643..11822)
                     /locus_tag="K8M77_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBI7085610.1"
                     /translation="MSQHLVPEAKNGLSKFKNEVAAEMGVPFSDYNGDLSSKQCGSVG
                     GEMVKRMVEQYEKGI"
     gene            12029..13081
                     /locus_tag="K8M77_001102"
     CDS             12029..13081
                     /locus_tag="K8M77_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBI7085611.1"
                     /translation="MTGGGSAGHVTPNLALVPKLKELGFEIKYIGSKNGIEKEIITKE
                     NIPYYSISSGKLRRYFDIKNFTDPFKVLKGVMDASRILSKEKPDVIFSKGGFVTVPVV
                     IAASMKKIPVVSHESDLTPGLANKIASPFCDTLCVTFPESLKYIKDNKGKLTGTPIRE
                     DLLKGDKERGRKFCNFKENKKVLMIIGGSLGSKIINESVRKILNEILKEYNVIHLCGK
                     GNLDESLKNLEGYRQYEYISEELPDLMALADLVISRAGANTIFELLALRKLNILIPLS
                     ANASRGDQVLNANSFEKSGYSMVIKEEELNSELLLKSIKDLEKNREKYLNSMKMSKIG
                     NGVNNIIDIIKKSAHM"
     gene            13288..13713
                     /locus_tag="K8M77_001103"
     CDS             13288..13713
                     /locus_tag="K8M77_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBI7085612.1"
                     /translation="MSKVSVIYWSNGGNVEVLANTIADELTDLGVEVLIKHVSNAKVE
                     DVIEADAVALGSPSMDNNRIEQYDMEPFVNQFKLTPNNNKKMILFGSYGWDNGEFMVE
                     WVKRMKDYGFDVVGSLAVNESPNDDEINSAKELAKKLIK"
     gene            13824..17333
                     /gene="nifJ"
                     /locus_tag="K8M77_001104"
     CDS             13824..17333
                     /gene="nifJ"
                     /locus_tag="K8M77_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBI7085613.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTDVAAIFPITPSSPMAEWVDENSA
                     RGLKNIFGQPVKVMEMQSEAGAAGAVHGSLQAGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVSARALATSALNIFGDHQDVMAARQTGFAMLAEGSVQEVMDLSAVAHLAAL
                     KARIPFVNFFDGFRTSHEIQKVELLQYDELKELVDMEAVEEFRRRALNPNKPVTRGTA
                     QNPDIYFQEREAVNKFYDAVPEIVESYMKEITKLTGREYNCFDYYGAADAERVIVAMG
                     SVTDLIEETVDYLNAKGEKVGLIKVRLFRPFSNERLIKAMPKTVKKVAVLDRTKEPGA
                     AGEPLYLEVKNAFYGLENAPVIVGGRFGLGSKDTVPADIVAVYENLNKEDAKNGFTLS
                     IVDDVTNTSLAPVGDIDTTPEGTKACKFWGLGSDGTVGANKSAIKIIGDHTDMYAQGY
                     FAYDSKKSGGVTISHLRFGKQPIKSPYLINKADFVACHNQSYVNKYFVLDGLKKNGTF
                     LLNTIWTPEEVAEHLPANYKRFLAENNIKFYTLNAVKIAQEVGLGGRINMIMQSAFFK
                     LANIIPVEDAVKYLKDAVVTSYGKKGEKVVNMNHAAIDKGIDAIVEITVPAEWANAKD
                     EVVEAKEVPAFIKNIVEPMNRLEGDKLPVSAFNGMEDGTFEPGTAAYEKRGIGINIPE
                     WIADNCIQCNQCAYVCPHATIRPFLLTEEEAKNAPASTKLVAAKALKTEEPMQFTMAV
                     STLDCTGCGNCAQVCPAKEKALVMKPQHTQEDQIEAWDYCVNDVSPKKNPMNKNTVKG
                     SQFEQPLFEFSGACAGCGETPYAKLITQLFGDRMMIANATGCSSIWGGSAPSTPYTTN
                     HNGHGPAWANSLFEDNAEFGLGMFLGVKAIRERLVDLAGKAIEAGVKPEAKEALEAWI
                     AEVDNGEGTRDRADAVVAALQGETNEFAKEILKDQDYLAKRSQWIFGGDGWAYDIGYG
                     GVDHVLASGEDVNILVMDTEIYSNTGGQASKSTPTAAIAKFAAAGKRTKKKDLGMMAM
                     SYGYVYVAQIAMGADKNQTLKAIAEAEAYKGPSLIIAYAPCISHGLKAGMGNSQLEEK
                     RAVECGYWAMYRFNPMLKEAGKNPFSLDSKEPTGDFREFIMGEVRYAALAKAFPEAAE
                     ALFEKTERDAKERLENYKKLAAN"
BASE COUNT         6939 a         1989 c         3376 g         5244 t
ORIGIN      
        1 ttttagattt attatttcat attttgttaa aagataacaa aaaaatatat tttacaaaat
       61 atataaacaa ctgttataat gtcagaggtt aatttaaatt ataggagtgg aatttatgga
      121 aaagtatata ttttgggtaa cagctatttt tcaaataggc gtatttgctc taatgctata
      181 ctatttattc atagcgttat ttggattcta taagaaaaaa gatacaaaga cttttgaacc
      241 tcaaaagaaa tttgcaatgc ttgtagcagc ccataatgaa gaagtagtta tagaaaatct
      301 agttgaaagt ttaaaacatt tagattatcc aaaagaatta tatgatatat ttgttattgc
      361 agataactgc actgatgata ctgctaagat tgcaagagca gcaggggcaa atgtatttga
      421 aagatttaat aaagagaaga gaggtaaggg tttcgcatta gaatggatgt ttgataaaat
      481 attcaaaatg gatactcatt acgatggaat agcagttttt gatgccgata acttagttga
      541 taagaatttc ttaaaagaaa tgaacaataa aatgtgtgaa ggatttaaag ttgtacaagg
      601 atatatagat tctaagaatc cacaggattc atggataaca gaatcttatg ccatagcatt
      661 tacatcagct aatagaatgc ttcaattagc taagagtaat ataggtttat cagctcaaat
      721 aggtggaaca ggattcgtta tagatactgc aactcttaaa aagttaggat ggggagctac
      781 ttgcttaaca gaagacttag agtttagttg taagcttata ttaaatggag aaaaagtagg
      841 ttgggcacat gatgccataa tatatgatga aaaaccatta actttaaaag cttcatggaa
      901 tcaaagaaag agatggatgc aaggatttgc agatgttgct actagatatg gaatgaaact
      961 tttaaagaaa tctataaaag atagaagctt agttgctttt gactgtactt tatatgtaat
     1021 gcaaccattt gttactttag caactggatt agctggttta ttaactttcg tacaaataag
     1081 agcagtagat ggtcttggaa tattcgttat aagttattta tttaatgcca cagtatggca
     1141 attattctgt gtattccaat tcttattaac accatttgct ttattgtttg ataaaaagat
     1201 gagcggaaag ttatttgtag ttttaggact atatgcatgt aacgtattta tacaaccata
     1261 cttaataaat caattaggta tattagacaa tataatgtta ttaacattat tcaatgtggc
     1321 ttatatagga atattcttaa taggaacatt attattccta ggaaagagaa atttccaaat
     1381 attctataga tatttaatct atccattata catattaact tggataccaa taacaatcca
     1441 aggtataata gataaagata aaaaagaatg gtcacacact aaacacgtta gacaaatcgg
     1501 aatatacgaa gtttaattat tataagacat ccattaatgg atgtcttata tttttgctgt
     1561 aaaaatgatt acgagagtat aaagttgaca atattattag aagatattta tttaaataag
     1621 gtaagttttt gtattttaaa atcttttgat ataattaata atcgtgaagt actataaaga
     1681 tatgttcgat gggaacaaat gtttgacaat gtatttttaa agaattatac tattttagta
     1741 aattgaaagg atgatatata aatgtttgga ttaataagtt atgaggagat atatggacat
     1801 gaggacgagt atatctttat agatgttaga agccaaaagg aagcctttga agaacctatg
     1861 atagggtctg taaatattcc agtgctttta aatgaagaga gagatgtagt cggaacttta
     1921 tatgttagag aaagtgcaga ggctgcaaaa gagcaaggaa tagaattcat ctctagaaga
     1981 ctccctgaaa tatttaaaca agttcaagag ctttatagaa ataagcatgg taaaaaactt
     2041 gttatttttt gtgcaagagg tggaatgaga agtggaagtc tacattcatt actaaatagt
     2101 ttaggaataa attgttataa actagaaggc ggatataaat catacagaag ttttataata
     2161 aataaaatag aagaattttc aaaggaaatt gattttatag tattatacgg aaatactggt
     2221 gtaggaaaaa cagaaatgtt atataaatta gaagaaaagg gatatgatgt tttagattta
     2281 gaaggatgtg caaatcatag aggttcaatt cttggaggag ttggattagg agattgcaat
     2341 actcagaaga gatttgatgc attagtttat gataaactta gacatagaaa ttctaatatt
     2401 gtattcattg aaggagaaag taaaagaata ggaagaattt taataccaga tcctttattt
     2461 gaaaagatgt atgaaggtgc aaaacttaaa ataagtgctg atccagaagt gagagcagag
     2521 cgattagtta aggaatatac tgcctttgaa aatgtaaatg aacaattaga tgaagcttta
     2581 ggagttctta agaagcatat aagtccagaa agagtggaac aatataaaga cttagtagcc
     2641 aaaggtgatt ataagaaagt agccttagaa cttatggaaa aatattatga tcctagatat
     2701 ggaacaagtg caaagaaaaa aacatttaga aatgaattag agataaatga catagaaaat
     2761 gaattattta aattagagga aatatataaa gagatttcag aggaattaag ggaggatgta
     2821 tatgagcaat aaaaataaca attatgatgt tacctcttta acttctttag aaaagttaga
     2881 accagtaaga gtaagaccag gaatgtacat aggttctaca ggaagcaagg gacttcatca
     2941 ttgtatatgg gaaatattag ataactctat agacgaaata tcaaatggat atggaaataa
     3001 ggctaaggtt attttaaata aagataaaag tgtaactgta atagataatg gaagaggaat
     3061 tcctacggga atgcatccaa taaagaaaaa aactggtgtt gaaatggtat ttacagagct
     3121 tcacactggg ggtaagttta ataaccaaaa ctataagact tcaggaggac ttcatggtgt
     3181 tggagcagcc gttgttaatg ccttatcaag atggctagaa gttgaagtaa aacaaaatgg
     3241 aaagatatat agacaaagat ttgaatatgc ttatgacaaa gagcttaaaa aagatatgcc
     3301 aggaacacca gtaactccat tagaagtagt aggagaatct aaggaaactg gaacaaaggt
     3361 tactttttta ccagatagag aagtattttc aacatcagat tttaaatttg acataataga
     3421 tgaaagatta caagaattag ctttccaaaa taaaggaata agattagaac ttattgataa
     3481 tagaaaagaa gaaagtgtaa gtaaagaata ttattctgaa agaggacttt tagattttat
     3541 agattattta aatgaaagta aaacccctct tcatccaact cctgttttat ttgaaggaga
     3601 aaaagaagta aataacatac aaatgtatgg agaaatatgt cttcaattta ctgattcaac
     3661 aacagagaat atagttagtt acgtaaataa tattcctact acagaggctg gtactcatga
     3721 gactggtttt aaaacaggaa tgactcgtgc ttttaaggaa tggactaaaa agcttaacct
     3781 tataaaagaa aaagataagg aatttgaagg cgatgattta agagaaggta tgacagctat
     3841 tgtcagaata aaaatcacaa atccagtctt tgaagggcaa acaaaaacta agcttgggaa
     3901 taatgaagct tacacaatga tgaatgattt agcttacact aaatttggtc attggataga
     3961 ggataataaa gaagttgcaa ctatgattat aaataatgcc ttagatgcag ctgctagaag
     4021 agaaaaaatt aaaaagatta atgatgcaga gagaaagaaa ataggaaaag gaacagcacc
     4081 tttagcagga aaggttgcag tatgcacatt aaaaaataaa gaagctaatg aattcatagt
     4141 agttgaggga gattctgcag gtggatcagc taagcaagct agagatagaa gattccaaac
     4201 aataatgcct tcaaaaggta aaataatgaa tacagaaaaa caaaagttag aaaatgttat
     4261 agcttctgag gaattaaaaa tctttaatac tgccataggt acaggaattt tagataatta
     4321 taatgaagaa gatttaaaat atgacaaaat aataataatg agtgatgctg acgtagatgg
     4381 atatcatata agaactcttt ggatgacata tatctataga tacatgagac ctcttatagc
     4441 taatggtcat ctttatttag cacaaccacc tctttataaa gttgctaaat caggtaaggg
     4501 taaagataag attctttatg cttatagtga tgacgaatta gaagaagtta aaaagaaggt
     4561 aggaaaggga gcaactattc aaagatttaa aggacttgga gaaatgaatc cagaccaatt
     4621 atgggagaca actttaaatc cagaaacaag aactttagta caagttacta tagaagatgc
     4681 cgctaaggct gaaaaaatgg tttctttatt aatgggtgat gttgttcagc ctagaaagaa
     4741 ctatatgtat aagtatgctg agttctaaag gaggagatta aatggctaag aagaatattg
     4801 aaatacctaa ggataataat attatcagaa tgcctcttga agaggttatg cctgataact
     4861 atttacctta tgcagtagaa gttgctaagg atagagcact tccagatgta agagatggat
     4921 taaagcctgt acacagaaga attttatatg gggcgtatat gttaaaggct tttccagata
     4981 aaccttatta caaatcagct agaatagttg gggatatact aggtaaatat cacccacatg
     5041 gagatagttc agtttatgat gctatggtaa ttttagctca aaattttagt acaagagctc
     5101 ctcttattga tggacacgga aactggggaa gtatagatgg agatggagca gcggctatga
     5161 gatatacaga ggctagactt tctagtatat ctatggagat gcttagagat atagaaaaaa
     5221 atgtagtaga catggttcca aactactcag attctgaaat ggaacctaag gtattaccag
     5281 ctagatatcc aaatctctta gtaaatggta cttttggtat agcagttgga ctttcaacta
     5341 atataccacc acataattta agagaagtta tagatggtac tttagcttat atagataata
     5401 atgaaattac tactagagaa ttaatgaact atataaaagg accagacctt ccaacaggag
     5461 gagttttaat tggtgagaaa actttattat ctgcttatga aacaggagaa ggaaaagtaa
     5521 ctttaagagc taaggctaaa attgaaactt tagaaaatgg aagacttgga atagtaataa
     5581 ctgaattccc ttatagaaga aataaggcta gaatacttca aacaatatca gatatgactg
     5641 gtgacaagag acatgctaaa gctttagatg gaatagtgga tattagagat gagtcagata
     5701 gaactggtat aagagccgta atagaattta agaaggctgt agatcacgat atggctgata
     5761 aggttcttaa gtatctttat aagaaaactg atcttcaagg taacataagc tttaatatgg
     5821 ttgccttagc agatggaaaa ccagagacta tgggattaaa aacaataata tctcattatg
     5881 taaaccatca aaaggatgtt gttacaagaa gaacaaaaag agagttagaa gtagcagaaa
     5941 agagatttca catagttgaa ggtttcataa aggctatagg aataatggat gaggttatag
     6001 ctacaattag agattcaaaa tcaaagaaag atgctcatga gaatttagtt ttaaaatttg
     6061 gatttactga cttacaggca gaggcaattc tagaattaat gctttataga ttaactggat
     6121 tagagataaa agtattccaa aaagagcata aagaattatc aaagaaaata aaagcactta
     6181 gaaaaatttt agaaaatgag tcagttcttt taggtgttat aaaagatgaa ttaaaagaag
     6241 tggctgaagt ttatggtgat gaaagaagaa ctgccttaat agaagatgaa agcgaagcta
     6301 agatagatct agaagagtta atagtagcag aagatgttat ggttactcta tcaaacgaag
     6361 gatttataaa gaagattcct cttaagactt ataatcgttc aaatattgat gaaaatgaaa
     6421 ttgaatatag agaaggagat tatttaaaat tccttattaa atcaaatact aaggatacct
     6481 tagctatatt cactgataaa ggaactgttt atcaaataaa atgtaattct gtagcagata
     6541 agaaatggaa agataaagga gaaagacttg aggatttaat aagaggatta agtttagaag
     6601 atgaaaaaat tattgctctt gaatcaattg aaaatttcct tccaaacaaa tgctttaaat
     6661 ttataactgc taatggatta ataaagaaga ctactttaga taaatttgtt actgcttact
     6721 caaaacttat ggctataaag cttaaaaatg acgatttatt agcaagtgta tctttaatag
     6781 attcacagga tgaagaaaga tttgttgaaa ttgaaacaac taatggatta aattttgttg
     6841 tttcagaacc agaattagag tttacagata gaaatatact aggagttcaa ttagtaccat
     6901 taaagagtgg taaccaaatt aagagtataa gatttgtaga taactatgaa tataaagaat
     6961 ttatcatagg aataaataaa aagggaaata taaaaacttt cagcaatatg aatagcaatt
     7021 cttatgaaaa ggtaaaggtt aattccttta gaaatataat tgctttctca aataaaggaa
     7081 aggtatttaa attcccagca tacttacttc aaaatacaga agaaagtaat atttcagact
     7141 tagtagatgg atttgaaaag gatgaactta taataaaggt agctcctata aatgaatttg
     7201 gaaaaatagg tgaagattta tttgtttact tcttctcaag agaaggatta gttaaaaaga
     7261 catctttaag agagttctta ggagaattta ataatcaaat agcttataag tttaaaactc
     7321 caaaggatga attagtaaat gtagatataa attttgaaaa tgctacagta atcttagtaa
     7381 ctaagaatgg tatgggaatt aagttcttag ctacagctat taatccaatg ggaagaatag
     7441 cttcaggggt aacaggaata agcttaaaag atgataataa agttatattt ggtaaggtta
     7501 taccaccatc tgaaggtatt gatgataaaa ctttagaggc gtataatgac tataaaaaag
     7561 aattaactag taattatgaa aaacttgttt tagagtctaa gcaaaaggaa aaagccgaag
     7621 tcaatattga agatattaaa ctacaaaata gagcgggaag aggaagtagt ttaatgattt
     7681 tagtattgga agactatata agggacgtaa ttattaagta ggaggtatgg tcatgaaaaa
     7741 agtattagta tttttagcag aaggttttga gactatagaa gcattgtctg ttgtagatgt
     7801 ttgtaatagg gctaaggtaa catgtcatgc atgtagtctt acagaaaata gaactgttaa
     7861 tagtgctcat ggaacaatgg tgctttgtga taaattaata tcagataatg atttagaaac
     7921 ttatgatgca attgtacttc caggtggaat gccaggatca acaaatctta gagataatga
     7981 gaaagtacaa tcattaataa agaaatataa tgaagaaaat aaaattgtgg ctgcaatatg
     8041 tgctgctcca atagctttag ctaaggctgg cgttattgaa ggaaagaagg ttacaagtta
     8101 tccaggattt aaagaggaac ttggaaatgt taattatgta gaagaagaca cagtagttgt
     8161 ggatggaaac acaataacta gtagaggacc tgcaaccgcc ttagtatttg gattagaaat
     8221 attaaaaaag ctaggatatg aaaaagaagc agaggaaata agagaaggaa tgcttattaa
     8281 ctttttctta gaaaaagaat gtaagtagaa cttttataat ttaaagagag ttgtatcaaa
     8341 ataacttaaa aataaaattt ataaacatat ttggaaaatt acttagttaa tctaagaagt
     8401 ttatagtaaa tattccattt ataaattttc ttttgagttt tgatacggct ctttttattt
     8461 tttacaagtt attaggtatg ttaaaatata ataagattta atatatttgg aggaaaatat
     8521 atgcaaaaaa taattttaaa taatggtgtt agattattat ataaatttaa agatatagaa
     8581 cacacatctt tttgcataag cttagagagc ggagctaatg tagaaaataa agaagaaata
     8641 ggtatggcac atgccctaga gcatattttg tttaaaggaa atgagaagct taaggaagat
     8701 gagataaatg aaaaattaga tgatttattt ggatttaata atgctatgac taatttccct
     8761 tatgttattt attatggaac tactgcaaaa gaagattttg aagaaggatt ttctttatat
     8821 gcagatatag ttttaaattc agatcttcag gaatttggtt tttcagaaga attaaatgtt
     8881 ataaaacaag aaagtgatga gtggaaagaa gatttagagc aacatgtaga ggatcttgct
     8941 ttaatgaatg gcttaccaga tgaaagaata ggaaacttaa taataggaga aaaaaatcat
     9001 atagaagcta taagttttaa agggttaaaa gatttttatg aaaaaaacta tttaagtgaa
     9061 aatatggttg tatcagttgt ttcttctctt cctttagaaa aagtaaaaga gattgtagag
     9121 aaaaatttta atagggcaaa gagaggaaaa atatctaaat atagtttaga aagaaatatt
     9181 aattgtggga tattctctaa aaagatagag gggaatactg gggcaaaaat atgctgttta
     9241 tttgatataa atgatttaag tatggaagag gtaactttac ttaaggtatt taatctttgg
     9301 tttggtgaag gggtaagctc cgtattatat gatgaaatta gaactaagaa tggactagct
     9361 tatgaagttt atagtgaagt taagtatgaa aagggaataa gattatttaa aatttatcta
     9421 ggaacctcta aggaaaagga agaagaggcc ttaggactta ttgaaaagtg tatatcaaag
     9481 gctatggata tagaagatta tttaagtgaa gagggattaa ataaactaat aaagagattt
     9541 aagcttaaaa attcattaga tttagaaaaa agtatagttc ttgctaatag aatggctata
     9601 tatgaaacta tgtttaatag aggagaatat atttttgaag aattaaactt aattgaaaat
     9661 ttaagtttaa aagatatgaa aaatttaatt aaaagagttt taaagaaatc cattgtgcaa
     9721 ataattaact aagaggtgat ggcgtgagag agcagtggct acttaaacaa agaaaaagag
     9781 atgaaataga aaatttaaag gaagaattaa atatagattt attaacatct attcttttgg
     9841 tgaatagaaa tataaaagat gaaaaagatg cacgtgaatt tttatttggt ggaattgaaa
     9901 atttaaatga cccatatctt atgaaagata tggagaaagg ggttaaaaga gtaaaagaag
     9961 caatagaaaa gggagataaa ataaccatat atggagatta tgattgtgac ggtgtatcaa
    10021 gtacttcaat tctttataaa gggttaaaaa gatgcaatgc taactttaat tatcatattc
    10081 cagataggga agatgaaggg tatggtatga actctaagag agttaaaatt ttaaaggaag
    10141 aaggcactaa ggtaatttta acttgtgaca atggaatagc agcctttgaa gaaatagatt
    10201 tagctgagaa attaggcatg gatgtaatat taacagatca tcatgatatt cctttagtta
    10261 aagatgaaaa tggagagata aataaagaaa ttccaaaggc ttatgctgtt ataaatccta
    10321 aacaggaaga ctgtaattat ccatttaaat ccctatgtgg agcaggaatt gcctttaaat
    10381 ttatatgtgc attatataag gaactaggga ttccacagga ggaagcttta gaattattag
    10441 agatttgtgc cattgctact gtgtgtgatg tagttgattt attaggggaa aatagaataa
    10501 tagtaaaaaa tggcttagaa atgcttaata atactaaaaa taaaggatta atagccttaa
    10561 agaaatatac aggtcttgag ggaagggaaa tggcacaata tcacttaggg tttgttatag
    10621 gcccttgtat aaatgccact ggaagacttg aaacagctga cttatctgtt gaattgcttt
    10681 tagctgaaga tgatgaaagg gcagatgctt tagctaagga gctttttgag cttaatcaga
    10741 gaagacagga attaactaaa gacagcgttg atatggtaat aaagcaaata gaagataata
    10801 atatggaaaa tgacaaggtt attttagttt ataattccta tatacatgaa agtattgctg
    10861 gaattgtagc agggagaata aaagaaaaat ataatgttcc tacaataata atgactaagg
    10921 gaaaagatat gcctaaggga tctgcaaggt ctatagaaga atataatatt tttgaggaat
    10981 tatcaaaatg taaagagcta ataagcaaat ttggaggaca tccaatggct gcgggtttat
    11041 ctgtagaaga aaaaaacata cctttattaa gaaagatgtt attagaaaac tgtaaattaa
    11101 cagattatga tataatacca aaggttagaa tagatgttaa gttaccttta gaaagtttaa
    11161 attatggatt agtagatagt ttagataagt tagaaccctt tggaaaagga aatgctagcc
    11221 cgctttttgg agagaaagat gttaaagtaa gcagggtttg gataatggga aaagagcaga
    11281 atgtaattaa atttagatgt aaattaagaa atagttacaa aaccatagat gccatagcct
    11341 ttggaaataa ggttgaggaa tttaaagaag attttgctaa taaatatgga gaagaggaat
    11401 tattaagagt attagatggt ggaaactgca actttagcat tgatttaatt tattacccaa
    11461 atataaatga atttaatggg aataaatcta ttcaagctaa tgtaaaatac tttagattat
    11521 aaaattaaag gagcaatatt ttaaaatatt gctccaatgt tttgtacata attaggcata
    11581 aattaatcat atacaacatt aatatttaat tttatttaac agtagacttt aataggttca
    11641 ggttaaattc ctttttcgta ttgttctacc attcttttaa ccatttctcc accaacgctt
    11701 ccgcattgtt tagaacttaa atctccattg taatctgaga atggtactcc catttcagct
    11761 gctacttcat ttttgaattt tgataatccg ttttttgctt ctggtactaa atgttgtgac
    11821 atttttcatt cctccttagt tattaattga gtttgtatta ctattaattg agtttgcatt
    11881 tatatattgt gcagaataga gtaaaatatg agagggaatt ataggagtta ttacaaaaaa
    11941 atatttataa aaccatagtt tatttaaaat attataaaat cacattttat taagggagtt
    12001 gatttttttg aaaaagtata aaataattat gactggtgga ggttcagcag gtcatgttac
    12061 cccaaattta gctttagttc ctaaattaaa agagttaggt tttgaaataa aatatatagg
    12121 aagtaaaaat ggtatagaaa aagaaataat aactaaggaa aatatacctt attattcaat
    12181 atcatcaggt aagcttagaa gatattttga tataaaaaac tttacagatc cttttaaagt
    12241 attaaaaggt gttatggatg cctcaagaat actttcaaaa gaaaaaccag atgttatatt
    12301 ttcaaaaggt ggctttgtta cagtacctgt agtaatagca gcctcaatga aaaaaattcc
    12361 tgtggtatct catgaatcag atttaacccc tggattagca aataaaatag catcaccatt
    12421 ttgtgatacc ctgtgtgtta catttccaga aagtttaaaa tatataaagg ataataaagg
    12481 gaaattaaca ggaactccaa taagagaaga ccttttaaag ggagataaag aaagaggaag
    12541 aaagttctgt aacttcaaag aaaataaaaa agttcttatg ataattggtg gtagtttagg
    12601 ttcaaaaatt ataaatgaaa gcgttagaaa aattttaaat gagatattaa aagaatataa
    12661 tgttatacat ttatgtggaa aaggaaactt agatgaatca ttaaagaact tagagggata
    12721 tagacaatat gagtatatat cagaggaact tccagattta atggctttag cagatttagt
    12781 tatatctaga gcaggagcaa atacaatatt tgaattatta gctcttagaa agttaaatat
    12841 attaatacct ttatcagcta atgcttcaag aggagatcaa gttttaaatg ctaattcttt
    12901 tgaaaagagt ggatattcta tggtcataaa agaagaagag cttaattcag aattattatt
    12961 aaaatcaata aaggatttag aaaaaaatag agaaaagtac ttaaattcca tgaaaatgag
    13021 caaaataggc aatggtgtta ataatatcat agatataata aaaaaatcgg cacatatgta
    13081 aatttttata gttaattctt agcatatggt agaagtttta caagaattaa gaataaacta
    13141 taaaaatatg tttcaaaata tcggaatatg aaacttgaaa aatttctgtc aaagagtata
    13201 attattaatg tcgagtaaaa ttagttttgt atcaaataat attgggcttt aagataaaat
    13261 atattatcaa gtataagagg tgtttttatg agtaaagtat cagtaatata ttggagtaat
    13321 ggtggaaatg ttgaagtttt agcaaacact attgcagatg aattaacaga tttaggagta
    13381 gaggttttaa taaaacatgt ttcaaacgct aaagtagaag atgtgataga agccgatgca
    13441 gtagccttag gtagcccttc aatggataat aacagaatag aacaatacga tatggaacca
    13501 tttgttaatc aattcaaatt aactccaaat aacaacaaaa aaatgatctt atttggatca
    13561 tatggttggg ataatggaga attcatggtt gaatgggtta aaagaatgaa agattatgga
    13621 ttcgacgttg ttggtagttt agctgtaaat gaatcaccaa acgatgatga aattaattca
    13681 gcaaaagaat tagcaaaaaa attaataaaa taatacttat gctaataagt aattttaatt
    13741 aattacttta aatatattat aatttttaag attttaattt atatatctac aaaattaatg
    13801 aaagagaggt catctcaatg gcaatgagaa aaatgaaaac tatggatggt aacacagctg
    13861 ccgcttatat atcatatgcg ttcactgatg ttgcagcaat attccctatc actccatcat
    13921 caccaatggc agagtgggtt gatgaaaaca gtgcaagagg attaaagaac atatttggtc
    13981 aacctgtaaa agtaatggaa atgcaatcag aagcaggtgc tgcaggagca gttcacggtt
    14041 cattacaagc tggagcttta actactactt acacagcttc acaaggttta ttattaatga
    14101 taccaaacat gtacaaaata gctggtgaat tattaccatc agtattccat gtatcagcta
    14161 gagcattagc tacttcagca ttaaacatct tcggtgatca ccaagacgtt atggctgcaa
    14221 gacaaactgg attcgctatg ttagcagaag gatcagttca agaggttatg gatttatcag
    14281 cagttgctca cttagcagct cttaaagcta gaataccttt cgtaaacttc ttcgatggat
    14341 tcagaacatc acatgaaata caaaaagttg aacttttaca atacgatgaa ttaaaagaat
    14401 tagtagatat ggaagctgta gaagagttca gaagaagagc tcttaaccct aacaagcctg
    14461 ttacaagagg tacagctcaa aacccagaca tctacttcca agaaagagaa gcagtaaaca
    14521 aattctacga tgcagttcca gaaatagttg agagctacat gaaagaaatc actaagttaa
    14581 ctggtagaga gtacaactgt ttcgattact atggagcagc agatgctgaa agagttatcg
    14641 tagctatggg atcagttact gacttaatag aagaaactgt agactactta aacgctaaag
    14701 gagaaaaagt tggtttaatc aaagtaagat tattcagacc attctcaaat gaaagattaa
    14761 taaaagcaat gccaaaaact gttaaaaagg ttgctgtttt agatagaact aaagaaccag
    14821 gtgctgcagg agaacctcta tacttagaag ttaagaatgc attctacgga ttagaaaatg
    14881 ctcctgtaat agttggtggt agattcggat taggatcaaa agatactgta ccagctgata
    14941 tcgttgctgt ttacgaaaac ttaaataaag aggatgctaa aaatggattc actttatcaa
    15001 tagttgatga tgtaactaat acttcattag caccagttgg agatatagat actactccag
    15061 aaggaactaa agcttgtaag ttctggggat taggatcaga cggaacagtt ggagctaaca
    15121 agagtgctat caaaatcatt ggagaccata ctgacatgta tgctcaagga tactttgcat
    15181 atgactctaa aaaatcaggt ggggttacaa tttctcactt aagattcggt aaacaaccaa
    15241 taaaatcacc ttacttaata aacaaagctg atttcgttgc ttgtcataac caatcatatg
    15301 ttaacaaata cttcgtttta gatggattaa agaaaaacgg aacattctta ttaaacacta
    15361 tctggactcc agaagaagtt gctgaacatt taccagcaaa ctataagaga ttcttagctg
    15421 aaaacaacat taagttctac actttaaatg ctgttaagat agctcaagaa gttggattag
    15481 gtggaagaat caacatgatc atgcaatcag cattcttcaa actagctaac ataataccag
    15541 tagaagacgc agttaaatac ttaaaagatg ctgttgtaac ttcatacggt aaaaaaggtg
    15601 aaaaagttgt taacatgaac cacgctgcta tagacaaagg aatcgacgct atcgttgaaa
    15661 tcactgttcc agctgagtgg gctaacgcta aagatgaagt tgttgaagct aaagaagttc
    15721 cagcattcat caaaaacatt gttgaaccaa tgaatagatt agaaggagat aaacttcctg
    15781 tatcagcatt caacggaatg gaagatggta ctttcgaacc aggtactgct gcatacgaaa
    15841 agagaggaat cggtataaac ataccagaat ggatagcaga taactgtatc caatgtaacc
    15901 aatgtgctta cgtttgtcct catgctacaa taagaccatt cttattaact gaggaagaag
    15961 ctaaaaatgc tcctgcttca actaagttag ttgctgctaa agcattaaaa actgaagagc
    16021 caatgcaatt cactatggct gtaagtactt tagactgtac tggatgtgga aactgtgctc
    16081 aagtttgtcc tgctaaggaa aaagctttag ttatgaaacc acaacatact caagaagatc
    16141 aaatagaagc ttgggattac tgtgtaaatg atgtttcacc taagaaaaac ccaatgaaca
    16201 aaaacacagt taaaggtagc caattcgagc aaccattatt cgagttctca ggagcttgtg
    16261 ctggatgtgg agaaactcca tatgctaaac ttataactca attattcgga gatagaatga
    16321 tgatagctaa cgctactgga tgttcatcaa tctggggtgg atcagctcct tcaactccat
    16381 acacaactaa ccacaatggt catggaccag cttgggctaa ctcattattc gaggacaacg
    16441 ctgagttcgg attaggtatg ttcttaggag ttaaagctat aagagaaaga ttagttgatc
    16501 ttgctggaaa agcaattgaa gctggtgtta aaccagaagc taaagaagct ttagaagctt
    16561 ggatagctga agttgacaac ggagaaggaa ctagagatag agctgacgct gttgtagctg
    16621 cattacaagg tgaaactaac gagttcgcta aagaaatatt aaaagaccaa gactacttag
    16681 ctaagagatc acaatggatc ttcggtggag acggatgggc ttacgacatc ggttacggtg
    16741 gagttgacca cgttttagct agtggagaag atgtaaacat acttgttatg gatacagaaa
    16801 tctactcaaa tactggtggt caggcttcta aatcaactcc aactgctgct atcgctaaat
    16861 tcgctgctgc aggtaagaga actaagaaga aagatttagg aatgatggct atgagctatg
    16921 gttatgtata tgttgctcaa atagctatgg gtgcagataa gaaccaaact cttaaagcta
    16981 tagctgaagc tgaagcatac aaaggaccat cattaataat agcttatgct ccatgtatca
    17041 gccacggatt aaaagctggt atgggtaact ctcaattaga agagaagaga gctgttgaat
    17101 gtggatactg ggctatgtac agattcaacc caatgttaaa agaagctggt aagaacccat
    17161 tctcattaga ttctaaagaa ccaactggtg atttcagaga attcataatg ggagaagtta
    17221 gatacgctgc attagcaaaa gcattcccag aagctgctga agctttattc gaaaagactg
    17281 aaagagatgc taaagaaaga ttagaaaact acaaaaaact tgctgcaaac tagtaagtag
    17341 tagataacta attacgttta atatattgat tgaagtataa agtaatataa gagttagagt
    17401 atttatgctc taactcttat ttttttgttt taatttagaa gtatattggt gttatataat
    17461 taagaaatat aatttataaa gcaaaaataa ttaataatct agttttataa aagataaaag
    17521 tataaatata aaattaaaat aagaatta
//
LOCUS       DAEVGI010000037        17297 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEVGI010000037 DAEVGI010000000
VERSION     DAEVGI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 17297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17297
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.037"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(153..473)
                     /locus_tag="K8M77_001105"
     CDS             complement(153..473)
                     /locus_tag="K8M77_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085614.1"
                     /translation="MFKKESRYITRGANEKLDLRLQLILWSMIDKLNEKGKELDYLQV
                     FKIRKCEEGILIEHSQEVPRYKEKYVIDLENIEINGNIKVFVIDDYKYATMMLAEEES
                     VKLL"
     gene            complement(531..830)
                     /locus_tag="K8M77_001106"
     CDS             complement(531..830)
                     /locus_tag="K8M77_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085615.1"
                     /translation="MPKKVEEKIIKSTRYCNLLRQASLKDEDYTYCIEKIFVKSMKRN
                     EIRFSVYKDTVRGDDIYVPRSLDVTELELMELIKKSIKEKVFSDDFIEMLKQELK"
     gene            complement(843..1181)
                     /locus_tag="K8M77_001107"
     CDS             complement(843..1181)
                     /locus_tag="K8M77_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085616.1"
                     /translation="MKKIYKESKLLEVNNIPSEVIERMKAIIDILNENYGDNRNIESD
                     LGGYVVIVENIVDIKIMKQDKLQGLIPEYTDIIECSEGVNWTSSLFLLSIDFSVVVIA
                     DEELSKILLE"
     gene            complement(1344..1985)
                     /locus_tag="K8M77_001108"
     CDS             complement(1344..1985)
                     /locus_tag="K8M77_001108"
                     /inference="COORDINATES: protein motif:HMM:NF024811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBI7085617.1"
                     /translation="MIKAVIFDLDGLLIDSEIVSYKIYKEILNQFGHSFSIEEYAQNF
                     SGKTEVKNVANLIDTYNLPWTVEVGLDNVLKVEDKFINEGIDLKTGVKKLLTYLKDNN
                     LKIAIASSSTRDRALTILRQHNIVEYFDEFVFGNEVEKGKPNPDIFLKACDKISVNPE
                     ECLVLEDSESGIQAAYSANIPVICIPDMKVPNQYYLNMTKAVLSSLEEVIYYL"
     gene            complement(2015..2644)
                     /locus_tag="K8M77_001109"
     CDS             complement(2015..2644)
                     /locus_tag="K8M77_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBI7085618.1"
                     /translation="MNNIFLKEPWLKWAIEIQSIAQCGLTYTKDIYDKERYEQLRNIA
                     AEMLSYKTDIPIDKIKTLFCNESGYQTPKLDTRAVIFKDEKILLVRENNGLWSLPGGW
                     VDVLESIESNTIKEVKEESGLDVTAKRVIAIQDRNKHNKPLYPYGVCKVFVECEVTGG
                     EFIENIETIEMNYFLVENLPNLSEEKVTSKQIEMCFEAKNNPNWQVMFD"
     gene            complement(2937..3716)
                     /locus_tag="K8M77_001110"
     CDS             complement(2937..3716)
                     /locus_tag="K8M77_001110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085619.1"
                     /translation="MKDEMIEFLIESGSEITGATTGAVIGGVVAGPAGAMLGIAGGKA
                     VEKVFSKLGSEIKNRVLSKRENNKIGATATYALEKIDENIKSGKKLRDDGFFENDDTN
                     RSTADEIVEGIMFSAQREHEEKKLKYYGNLVANIAFDKTISREQANQLINISKRLTYR
                     QIKLINLFAINQQLPIQILRKEDYTNGGVSGCTLISVLQDTLELYNIGLVGGNGSVIL
                     EMPYIAPADIRVQGMGAMLYNLMELSKMPYTELEDLIEVLK"
     gene            complement(3788..5485)
                     /locus_tag="K8M77_001111"
     CDS             complement(3788..5485)
                     /locus_tag="K8M77_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7085620.1"
                     /translation="MGFKKLVNDTKIEKRRKIIAIYCRVSTDEQAEFGYSIDEQKRLL
                     EEWCKANDYIIYKCYSDRGISGKNIKDRPALKELLSDAKEGKFDMVISWKINRVSRKL
                     EDVLKIVNLLEKNNITFKSYSEPFETDTPAGRMQFQMMALIGEFERGTIAQNVKMGMI
                     AKAKSGNWCGGRVLGYDLVPNNSPEEEKKGKNKLEINEKEAEIVRFIFNEYSKGKGYK
                     AITNKMNKLGYKTKKGNNFSVGSIRDILTNPVYIGEIRYNVRQNWSEKRRRNINPNPI
                     RVKGKHEAIIDRELWDKVQLILESKKGKPSRIYDGEYPLTGILRCPKCGAGMVISRTT
                     NTLADGTKKRIAYYCCGNWKNKGTSVCNSNTIRVDKANEYVFKKIEELVSNEAMIKAV
                     VKNINKERKDKVKPAKRLLGDIDKELEKLDKRKRKIFEAYEDDILTKEEFQTRKNELN
                     EKIRILEEEKKPLLNTISEEVSEEIPYEFIKDILMNFSKILNSSVSREQQKKLLHMII
                     SEITMNESREIESIKLNINDKLVEYLVKEGGVPIKGIPSSFMLINVGLKVLNLDVVI"
     gene            complement(5625..6037)
                     /locus_tag="K8M77_001112"
                     /pseudo
     CDS             complement(5625..6037)
                     /locus_tag="K8M77_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6263..8749)
                     /locus_tag="K8M77_001113"
     CDS             complement(6263..8749)
                     /locus_tag="K8M77_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085621.1"
                     /translation="MNKNYKEFKQECTKAIKVLGADKRAVEIRVIKTKKGTVSGYYDD
                     SEKLLRDIFKYDGVNNIFFTLNKFSEDLLARGKDRLIEYASHTTSDSEITRREFLLID
                     VDPKRPAGVSSTDEELQSSEAVLREVVSYLRSEGFPEPVIACSGNGYHALYKLDLPNT
                     KEVTQLIKDFLYALDEKFSDDKAQVDKTTYNPARITKMYGTIACKGDSTETRPHRRSK
                     IVQSPDKLEVVKEDLLKKVADLYINKKNIDKNVKNVHIKSKSKGDIKIEEWLTDNGLN
                     VFKVKEESDRTVYVLDTCPWNSNHTDKSASITQFNNGAISAKCHHDSCSHENWSSLKK
                     LYEPKLSKNKSEYSDNNKGESKKSYADIVIEQALNYGDVFFHNSIDETFVAIDKNKFY
                     EIHKIEDNKYQMLLRKRFYDEVGKSVSKDNINQAIGVLEAKALYEGDELEVYKRCAEV
                     NGTIYYYLCDKNSTIIRIDEEGWKVDDSKKILFIRKNNMSEQVMPEPYEDLMGILSKH
                     FRFKSEEDKILHAVSLVTRLIPNIPHPIEVIHGEKGSSKTTTMKMNRSLIDPAPRDII
                     SIPKATQDLAITISNNYMPCFDNLDYLSAEKSDLLCIASTGGGYSKRKLYSNDDEIIV
                     NFKSRITLNGINVIATRADLLDRCIVLTLERIPEYERKEEKEVWENFNEDKPKIVGAM
                     FNTLAKAIPIYRNIELEKLGRMADFTKFGYAIAEACGIGGERFLEAYLNNQRKANQEA
                     VDSNPIASALIKYMNENIKFTGTVSTLLTVLNQVAEKEKIDTTSKLWAKEPNVLSRRL
                     NEIKSNLELEGIYYEITQRNHGRVIEVIKQ"
     gene            complement(8872..9999)
                     /locus_tag="K8M77_001114"
     CDS             complement(8872..9999)
                     /locus_tag="K8M77_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085622.1"
                     /translation="MCKTRHTDDEKRECVNEYLNGKDKYYLAGKYSISIRTIDRWIAK
                     INTNEIELRKRRRYGCIGLNIQDEILKSLNTNPIIKGFKISQWNEAITIKYIKDKYNI
                     SINRRMAKNLIEDSKNINDISYEDRVYNEIEEMESLGYNIVLLDYIKIGRIASREVEC
                     LELRKYTEEKLDVNLVVIRANNNMYLDIILSEAKVVDKSPRVNIRELSNKFKNNDEYE
                     RIVNIRKQQRKDIVNDKYNILDKVFKEERNKKIVFTTKCDKDIKRILGRKNNFKFYII
                     EDEVYNDLRQNIYEGEYGDNAIYYFYDENNTGRMYRNIREISQVVKGKIERYVLNSTI
                     DNYKLKNGVNYVDENDSNIQKNKDKLRKKIKILKSYFIHNI"
     gene            complement(10159..10617)
                     /locus_tag="K8M77_001115"
     CDS             complement(10159..10617)
                     /locus_tag="K8M77_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HBI7085623.1"
                     /translation="MYCKECGNKFKNEKAVVCVHCGTNKGQGNNYCSECGTEVKNKEG
                     AEVCLNCGCKLKNSKINISNSLKGSNGTGKPIGNSKVVAGISAILLGAIGIHNFYLGY
                     KEKGFIQVAIFLIAIIFFEPLVLVNNVWSIVDAVNIFRGKMKTATGEELV"
     gene            complement(10621..10905)
                     /locus_tag="K8M77_001116"
     CDS             complement(10621..10905)
                     /locus_tag="K8M77_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HBI7085624.1"
                     /translation="MSKKSENYSGIYKDMVEVLGEEITLKIYENYRGQQVTFPMRLYS
                     KSYILEYLNKNYNGKNLKELSRKLGYTCNWLQQVISKEIIREKNKEKVEV"
     gene            complement(11636..11992)
                     /locus_tag="K8M77_001117"
     CDS             complement(11636..11992)
                     /locus_tag="K8M77_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085625.1"
                     /translation="MKELSKEKSFEYSSKELLGVMRFDFYDGGLANQWNPRDLIIELN
                     DKKEIDLKKLQKELNYIQFDLIKSFDTVVRFCNGRGYDNETLVYIDLEIAKYVIKLVP
                     VRDSYSYIYAYLKEVR"
     gene            complement(12004..12309)
                     /locus_tag="K8M77_001118"
     CDS             complement(12004..12309)
                     /locus_tag="K8M77_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085626.1"
                     /translation="MFKKESRYITRGANEKLDLRLQLILWSMIDKLNEEGKELDYLQV
                     FKVRKCKEGIVIEHSQEVPEYKEKYVIDLENIEVNGNIKVFVIDDNEYSTMMLAEEY"
     gene            complement(12367..12669)
                     /locus_tag="K8M77_001119"
     CDS             complement(12367..12669)
                     /locus_tag="K8M77_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085627.1"
                     /translation="MSKNIKEDKIIKETKYCKIINQGKVGDDEYTYTIEKIYIKELKR
                     NEIRFCVYKSTRRGDETYIPRSLDVTELELMELIKKSIKEKVFSDEFIEMLKQELK"
     gene            complement(12687..13016)
                     /locus_tag="K8M77_001120"
     CDS             complement(12687..13016)
                     /locus_tag="K8M77_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085628.1"
                     /translation="MKKVYLKNQLKGYPIEVISNVLDVINVLGENYGANRDIEKDLGG
                     YIVIAENIVVIEMLKKDKLQGLIPEYTDIIECKEGVNWTSSLFLLSSDYAIVVVTTEE
                     LSKFLLE"
     gene            complement(13246..14472)
                     /gene="tetA(P)"
                     /locus_tag="K8M77_001121"
     CDS             complement(13246..14472)
                     /gene="tetA(P)"
                     /locus_tag="K8M77_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter TetA(P)"
                     /protein_id="HBI7085629.1"
                     /translation="MVNKLSAYKTYLLFSAITAMCFSLVATVMIVYHIEIVHLNPLQL
                     ILVGTTLELACFIFEIPTGIVADVYSRKLSIVIGGVLTGVGFILEGSISSFVFVLVAQ
                     IVWGLGSTFISGSLEAWIAEEEKNKDLDEIYIKGAQAGQIGAFIGIVLSTVIANFSVR
                     LPIIVSGVLFIILALFLWLYMPENNFKPSAPGDLNTFKKMVYTFKSGLKFVKSKSIIM
                     ILLAVTLFYGLSSEGYDRLSNAHFLQDTTLPKLGNLSSVTWFGIFGILGMILSFIVMH
                     FMAKNLKNEDNRKNGKLLLCINILYISSMLIFALTRNFSLMLIAYLATNTFRIINEPI
                     FSAWLNGHIDDNSRATVLSINGQMNSLGQILGGPIIGIIATNISVSIGIVCTSLLVAP
                     VLVLYIISMIMDKKIS"
     gene            complement(15208..15687)
                     /gene="rlmH"
                     /locus_tag="K8M77_001122"
     CDS             complement(15208..15687)
                     /gene="rlmH"
                     /locus_tag="K8M77_001122"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBI7085630.1"
                     /translation="MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTP
                     DNASEKEELQIKEKEGKLILSKIKDNMHVIAMDLKGNEITSEKFSKYIENCGVMGNSN
                     ITFVIGGSLGLSQEVIKRADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK"
     gene            complement(15933..16304)
                     /locus_tag="K8M77_001123"
     CDS             complement(15933..16304)
                     /locus_tag="K8M77_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBI7085631.1"
                     /translation="MSKELIIAIIFMNLALLFYSIGVWSEKIQKTLKPWHLAFFYLGL
                     ICDTTGTGAMEKIAGGINFSFHTITGGLALILMLVHAIWATVVLVKKDKNMIAKFHKF
                     SIIVWVIWLIPFFTGAAMHML"
     gene            complement(16535..17176)
                     /locus_tag="K8M77_001124"
     CDS             complement(16535..17176)
                     /locus_tag="K8M77_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBI7085632.1"
                     /translation="MDKNVMWLRECRINKVIKALEENNINGYLVNSKEELISKIEELT
                     NSGDIVACGGSMSLFEAGVIDHLRSGRYEFLDRYKEGLKPEDIKKIYRGAFSSDVYFT
                     STNAITEEGEIYNVDGNGNRVAAMLYGPDKVIIVAGYNKIVENLSEAIQRNRRISAPA
                     NAKRLNKKTPCAKVGNCMDCKSPEKICREYTLIRSQSNSNRIHVIFLNEELGY"
BASE COUNT         5442 a         3178 c         1462 g         7215 t
ORIGIN      
        1 aattggttta ttgctgctta aattattaat atctttgatt aacctgcaaa aaaatcaaat
       61 catcttgcag gtttgtttgc catgcaaaaa tttattcaaa cttatataat tttcaaagaa
      121 cgagtaattt tacaaatagt taagctattt tttcataaca acttaacaga ttcttcttct
      181 gctaacatca tagttgcgta tttataatca tctataacga aaacttttat atttccatta
      241 atctctatat tttctaaatc tataacatac ttttctttgt accttggtac ttcttgacta
      301 tgttcaatta aaattccttc ttcacatttt cttatcttga aaacttgtaa gtagtctaat
      361 tccttacctt tctcattcaa tttatcaatc atactccaaa gtataagttg taatcttaaa
      421 tctaattttt cattagctcc tctcgttata tatctgctct ctttcttaaa catcttaatc
      481 ctccttaaaa taaataaagc tacctaatta cttttcttgt aattaagtag ctattttaat
      541 tcttgcttta acatttctat aaaatcatct gaaaaaactt tctctttaat tgacttcttt
      601 attagctcca ttaactctaa ttcagttaca tctaaacttc ttggaacata gatatcatca
      661 cctcttacag tatctttata gaccgaaaat cttatctcat ttcttttcat agatttaaca
      721 aaaatctttt ctatgcagta tgtgtaatct tcatctttta gactagcttg tcttagaaga
      781 ttacagtatc tagtactttt tattattttc tcttcaactt ttttaggcat actatcaccc
      841 ctttattcta aaagtatctt agaaagttct tcatcagcta ttactactac tgaaaaatcg
      901 atagaaagta aaaatagtga agaagtccaa ttaactcctt cactacattc aattatatca
      961 gtatattccg gtattaaacc ttgtaattta tcttgtttca ttattttgat atctacgata
     1021 ttttctacta taactacata tccacctaaa tcagattcta tattcctatt atctccataa
     1081 ttttcattta gtatatctat tattgctttc attctttcaa ttacttctga tggtatatta
     1141 ttcacctcta ataatttact ttctttataa atcttcttca tcatcaatcc tccctaaaat
     1201 acaaaaactt ctatatgtaa tctaattaca catagaagtt tcttgtgagt aatatctttg
     1261 tttagttgtt attcttaatt ataatatata tttatatttt agaattattt cgtactatat
     1321 taaaattgtt cattaaatct actctataaa taataaataa cttcttctaa tgaagataat
     1381 actgcttttg tcatattcaa ataatattga tttggaactt tcatgtctgg tatacatatt
     1441 actggaatat tagctgaata tgctgcttga attcctgatt cactatcttc taaaactaag
     1501 cattcttctg gatttactga aattttatca caagctttta agaaaatatc tgggtttggt
     1561 tttccttttt caacctcatt accaaataca aattcatcaa agtattcaac aatgttatgc
     1621 tgtctcaata tcgttaatgc tctatctctt gtacttgatg aagcaatagc aatttttaaa
     1681 ttgttatctt ttaaatacgt taataatttt tttactccag tctttaaatc aataccttca
     1741 ttaataaact tatcttcaac ttttaatacg ttgtctaatc ctacctcaac agtccacggt
     1801 aaattatatg tatctattaa atttgcaaca ttcttaactt ctgtttttcc gctaaaattt
     1861 tgagcgtatt cttcaataga aaagctatgt ccaaattggt ttaatatttc tttatagatt
     1921 ttatatgaaa ctatctcact atcaattaat aaaccatcta aatcaaatat aactgctttt
     1981 atcatatatc tctcccctta aatacatata tatattagtc aaacattact tgccaatttg
     2041 gattattttt agcttcaaaa cacatttcga tttgtttaga tgtaaccttt tcttcagaaa
     2101 gatttggaag gttttcaact aaaaaataat tcatctctat agtctctata ttttcaataa
     2161 attctccacc agtaacttca cattcaacaa aaaccttaca cactccataa ggatataacg
     2221 gtttattatg tttatttcta tcttgaatag caataactct ttttgcagta acatcaagcc
     2281 cagattcttc ttttacttct tttatagtat ttgattctat tgattctaaa acatctaccc
     2341 aaccaccagg tagtgaccat aatccgttgt tctcacgaac aagtaatatt ttctcatctt
     2401 taaaaattac tgctctagta tctaatttag gggtctgata accactttca ttacagaata
     2461 aagtttttat tttatcaata ggaatatctg ttttatagct taacatttca gctgcaatat
     2521 ttcttaattg ctcatatcgt tctttatcat atatatcttt tgtatatgtt aatccacatt
     2581 gtgcaatact ttgtatctca attgcccatt ttaaccaagg ttcttttaaa aatatattat
     2641 tcataatatc accatccaaa ctttagacta tattatctat atattactat ttcgcattat
     2701 aatacattta ttcattactt tatattttta ttataccata ttattctatt tttaaataaa
     2761 tacatcaagt tattatttta caatagctta attactttat aaaaaaactc ttacctgtag
     2821 tttaatacaa gtaagagttt taatatctta taacattatt ctatttacaa tttcagtaaa
     2881 aaccgcattt atttatgata cggttctccg tatcagtatt aagtgaggtt ttttttctat
     2941 tttagtacct ctattaaatc ttctaattct gtatacggca tctttgataa ctccattaaa
     3001 ttatataaca tagctcccat accttgaact ctaatatcag ctggcgctat atatggcatt
     3061 tctaaaataa cactaccatt tccaccaact agcccaatat tatacaattc aagtgtatcc
     3121 tgtaatacag atatcaaagt acatccactt actccaccat tcgtatagtc ttcttttctt
     3181 aatatctgaa ttggtaactg ctgatttata gcaaataaat ttattaattt tatttgtcta
     3241 taagttaacc tctttgatat attaattaat tgattagctt gttccctact tatagtttta
     3301 tcaaatgcaa tattagctac taaattacca taatacttta gctttttttc ttcatgttct
     3361 ctttgagctg aaaacattat accctctaca atttcatcag ctgttgacct attagtatca
     3421 tcattctcaa aaaatccatc atctcttaac ttcttaccac tttttatatt ttcatctatt
     3481 ttctctaacg catatgtagc tgttgctcct attttattat tctctctttt tgataatacc
     3541 ctattcttta tttctgaacc tagctttgaa aatacttttt ctacagcttt tcctcctgct
     3601 atcccaagca tagctcctgc tggtccagca acaactccgc caatgactgc accagtagtt
     3661 gctccagtta tctctgaacc tgattctatc aaaaattcaa tcatctcatc tttcattcta
     3721 caatacactc ctcaaaataa ttagtattaa tataattata ctatattaat gctaattgaa
     3781 taataatcta tataacaaca tctaaattca ataccttcaa tccaacattt attaacataa
     3841 aagaagaagg aataccttta ataggtactc ctccttcttt tactagatat tcaactagct
     3901 tatcatttat attaagtttt attgattcta tttctcttga ttcattcatc gttatttcag
     3961 atattatcat atgtagtaac tttttctgtt gctctctact tacactacta tttaatatct
     4021 tactgaaatt cataagtata tctttaatga actcatatgg tatctcttca cttacctcct
     4081 cagaaattgt gtttagtaac ggcttctttt cttcttctaa aattctgatt ttctcattta
     4141 gttcattctt tctagtttga aactcctctt tagttaaaat gtcatcctca tatgcttcaa
     4201 agatttttcg ttttcttttg tccagttttt ctagctcttt atctatgtca cctaacaatc
     4261 ttttagctgg tttaacttta tcttttcttt ccttattaat atttttaaca actgctttaa
     4321 tcattgcttc attagatact aattcttcaa tctttttaaa cacatattca ttagctttat
     4381 ctactcttat tgtattacta ttacatactg aagttccttt attcttccaa ttaccacaac
     4441 agtaataagc tattctcttt ttagtaccat ctgctaatgt attagttgtc ctagaaatca
     4501 ccataccagc tccacactta ggacatctta gtattcccgt taatggatat tctccatcat
     4561 atattcttga gggctttcct ttcttacttt ctaaaattaa ttgaacttta tcccatagct
     4621 ctctatctat aatagcttca tgctttcctt taactcttat tggattagga tttatattcc
     4681 tccttctttt ctcactccaa ttttgtctta cattatatct tatttctcct atgtatactg
     4741 gattagttag tatatctctt atacttccta ctgaaaagtt atttcccttt ttagttttat
     4801 atcctaattt attcatttta ttagttattg ctttatatcc ctttccttta ctatattcat
     4861 taaatataaa ccttactatt tcggcttctt tttcattgat ttctagtttg tttttcccct
     4921 ttttttcttc ttctggtgag ttatttggta ctaagtcata tcctaagact cgtccaccgc
     4981 accaatttcc agattttgcc tttgcaatca tccccatctt aacattctgt gcaatagtcc
     5041 ccctttcaaa ctctcctatt aaagccatca tttgaaattg cattcttcct gctggtgtat
     5101 ctgtttcaaa aggttctgaa taagatttga atgttatatt gtttttctct aatagattta
     5161 caatctttaa aacatcttct aactttctac ttacacggtt tatcttccaa gatataacca
     5221 tatcgaactt tccttcttta gcatcactta aaagttcttt taatgcgggt ctatctttta
     5281 tgttttttcc acttattcct ctatcagaat aacatttata gattatgtaa tcattagctt
     5341 tacaccattc ttctaaaagt cttttttgtt catctatact atatccaaat tctgcctgtt
     5401 catcggtact tacccggcaa tatatagcaa ttattttcct ccttttttca atttttgtat
     5461 cattaaccaa tttcttaaat cccatagaac tcctcctaat atctaatcaa atttataata
     5521 tatacttaac tttctatttc actaccctaa acttattatg gaaaatatca tagtaattac
     5581 atttcgatat ttcaatactt cgatattttc cattgttaat taatctatat aaaatcagat
     5641 aattttaaaa attctttctc aactcctaat ggttcaaatt tatatatgaa tatataatct
     5701 ttcaacctaa attttctcat tactttttta acatttctcc tacattacca atgtcattac
     5761 aacattcctc ttgactaaac ccatatgaac ctattaagtt aaaatcaaaa aaattaatta
     5821 ctattttatc tttttcttcc ctgtttaaac ttacatggta gatacctgtt gaacaataca
     5881 attctgtaat taacttttct atccatcctt cttttcttct acttctttta tttttcataa
     5941 taactaactt attatgaaca gctacccata cacttttatt ttttaactcc atgtattcac
     6001 ttgagtcaca ttcaaatttt actttaatat tattcattgc taatctctcc tccatatatg
     6061 cttattaatt tacattatat ttttaattat tttcttcttt tacacactct ctagtaatat
     6121 aattaattat tatttctgaa attaaatcta ttatttcttg gcaactatta atattattca
     6181 ttttactaac tccttttatc tttaaattat cgacattatc gaaatatcaa ctccgataat
     6241 gtcttatatt ataaaaatct aattactgtt taattacttc aatcaccctt ccatgatttc
     6301 tttgagttat ttcataataa attccttcta attctaaatt agacttaatt tcatttaacc
     6361 ttctacttag aacattaggc tctttagccc atagtttgga tgttgtatca attttctctt
     6421 tctcggctac ttgatttaaa acggttaata atgtacttac tgttcctgtg aactttatat
     6481 tttcattcat atacttaatt aatgcacttg ctattggatt ggaatcaact gcttcttgat
     6541 tagcttttct ttgattattt aaataagctt ctaaaaacct ttcccctcca attccacaag
     6601 cttctgctat cgcatatcca aatttagtga aatcagccat tctgcccaac ttctctaact
     6661 ctatatttct ataaattggt atagcttttg ccaatgtgtt aaacatagct cctactattt
     6721 ttggtttatc ttcattaaaa ttctcccata cttccttttc ttcttttctt tcatattccg
     6781 gtattctttc taatgtcaga actatacatc tatctaaaag gtcagctctt gtagcaataa
     6841 catttatacc attcaaggta atacgacttt taaaattaac tattatttca tcatcgttac
     6901 tataaagttt ccttttacta tatcctccac cagttgaagc aatacataat aaatcacttt
     6961 tttctgctga aagataatct aagttatcaa agcatggcat atagttatta gagattgtta
     7021 ttgctaaatc ctgagttgct ttaggtattg aaattatatc tctcggtgct gggtctatta
     7081 aacttctatt cattttcata gtagttgtct tagaagaacc tttttctcca tgtattactt
     7141 caattggatg aggaatatta ggtattaatc tagtaacaag acttacagca tgaagtattt
     7201 tatcttcttc tgacttaaac ctaaaatgct tacttaatat tcccattaaa tcttcataag
     7261 gttcaggcat tacttgctca ctcatattat tctttcttat gaataatatt ttctttgaat
     7321 catctacttt ccacccttct tcatctattc ttattattgt agaattcttg tcgcataagt
     7381 aataataaat agttccattc acttctgcac atctcttgta aacctctaat tcatctcctt
     7441 catataaagc tttagcttct aaaactccta ttgcttggtt aatattgtcc ttagaaacag
     7501 acttaccaac ttcatcataa aacctctttc ttaaaagcat ttgatattta ttatcctcaa
     7561 tcttatgtat ttcataaaat ttatttttat ctattgctac aaaagtttca tctatggaat
     7621 tatgaaaaaa tacatctcca taatttaatg cttgttcaat tactatgtct gcataacttt
     7681 ttttagattc tcctttatta ttatcagaat attctgattt atttttcgat aacttaggct
     7741 cataaagctt ttttagagaa ctccaatttt catgtgaaca actatcatga tgacatttag
     7801 cactaatagc tccattatta aattgtgtta ttgaagctga tttatctgta tggttagagt
     7861 tccatggaca tgtatcaagt acatatacag ttctatcact ctcctcttta accttaaaaa
     7921 catttaatcc attatctgtt aaccactcct caatctttat atctccttta ctttttgatt
     7981 taatatgtac atttttcaca ttcttatcaa tattcttttt attaatgtac aggtctgcta
     8041 ctttctttaa taaatcttcc ttgactactt caagtttatc tggagattgt actatttttg
     8101 acctcctatg aggtctagtt tcagtactat ctcctttaca agctattgtt ccatacatct
     8161 tagttattct tgctggattg tatgttgttt tatctacttg tgctttatca tcactaaact
     8221 tttcatctaa agcataaaga aaatccttaa ttagttgagt tacttcttta gtattaggta
     8281 aatctaattt atataaagca tgatatccat taccactaca agcgattaca ggttcaggaa
     8341 atccttcact tcttaaatag cttacaacct ctcttaatac tgcttctgat gattgtagtt
     8401 cttcatctgt tgaagatacc cctgccggtc ttttggggtc tacatctatt aataagaatt
     8461 ctcttcttgt aatttccgaa tctgatgttg tatgacttgc atactctatt aacctatctt
     8521 ttcctcttgc aagtaaatcc tctgagaatt tatttaatgt aaagaagata ttattaactc
     8581 catcatattt aaaaatatct cttagaagct tttcactatc atcgtagtaa ccacttactg
     8641 ttcctttttt agtcttaatc actcttattt caactgctct cttatcagct cctaaaacct
     8701 ttattgcttt agtacattct tgtttaaatt ctttataatt cttgttcatc cacatatcct
     8761 cctaatcttt tttgctcctc gctacaagaa ttattataaa gtgatagttc ccttttctca
     8821 tctcactgga attaatttca aaataaagga cagaggagaa actcctctgt cttaaatgtt
     8881 atgtataaaa tatgatttta aaattttaat cttttttctt aatttatctt tatttttctg
     8941 tatattacta tcattttcat ctacataatt aactccattt ttaagtttgt aattatctat
     9001 agtactattt aagacatatc tttctatttt accttttaca acttgactta tctccctaat
     9061 atttctatac attcttccag tattattttc atcgtaaaaa taatatattg cattgtctcc
     9121 atattctccc tcataaatat tttgtcgtaa atcattatat acttcatctt caattatata
     9181 gaatttaaaa ttattcttcc tacccaatat ccttttgata tctttatcac atttagttgt
     9241 aaatacaatt ttcttattac gttcttcctt aaatacttta tctaaaatat tatatttatc
     9301 attaacaata tcttttcttt gttgttttcg tatatttaca atcctttcat actcatcatt
     9361 atttttaaat ttattactta gctctcttat gttaactcta ggagatttat ctacaacttt
     9421 tgcttccgat aaaattatat ctaaatacat attattatta gctcttataa ctacaagatt
     9481 aacatctaac ttctcttctg tatattttct aagttctaga cattctactt ctcttgaagc
     9541 tattctccct atctttatat agtctaataa aactatgtta taacctaaac tctccatctc
     9601 ttctatctca ttatataccc tatcttcata gcttatatca ttaatatttt tagaatcttc
     9661 tattaaattt tttgccattc tcctattaat acttatatta tacttatctt ttatatattt
     9721 aattgtaatt gcttcattcc attgagatat tttaaatcct tttattattg gattagtatt
     9781 taaactttta agaatttcat cttgtatatt tagaccaata caaccatatc ttcttctctt
     9841 tcttaattca atctcattag tattaatttt agctatccat ctatcaatag tcctaattga
     9901 tatagaatac ttaccagcta gatagtattt atccttacca tttagatatt catttacaca
     9961 ttctcttttt tcatcatcag tatgtcttgt tttacacata ttataaatac tcacctctga
    10021 ttaatttcta tatgattata gcatttcatc ttaacacttc caactttctt cttgaaaata
    10081 tcatcactat cgtaatatag attgtaacga tattttctgt aaaaaaaaag aggtgttacc
    10141 ctcttttaaa aaatatattt acactaattc ctcacctgtt gcagttttca tttttcctct
    10201 aaaaatattt acagcatcta ctattgacca tacgttatta actaaaacta aaggctcaaa
    10261 gaatattatt gctattaaaa atattgctac ttggataaat cctttttctt tatagcctaa
    10321 ataaaaatta tgaattccta tagctcctaa caatattgct gaaataccag ctactacttt
    10381 agaatttcct attggtttac ctgtcccatt acttcctttt aaactattag atatgtttat
    10441 ttttgaattc tttaatttac atccacaatt taaacatact tctgctcctt ctttattctt
    10501 aacttctgta ccacattcac tacagtaatt attaccttgt cctttatttg ttccacaatg
    10561 aacacataca acagcttttt cattcttaaa tttatttcca cattctttac aatacataat
    10621 ttatacctcc actttttcct tatttttttc tctaattatt tccttactta taacttgttg
    10681 tagccaatta cacgtatatc ctaattttct tgacaactct tttaagtttt taccattata
    10741 gtttttattt aagtattcta atatataact tttagaatat aatctcattg gaaaagttac
    10801 ttgctgacct ctgtaattct catatatctt taatgtaatc tcctctccca atacttccac
    10861 catatcttta taaataccac tataattttc actcttttta ctcatacata tatttactcc
    10921 tccttttcat ttataatata aataataacc tcacttacat tttttgtaat gttgattata
    10981 tatgtgattt tctattgtaa aatcacatat ataatttcaa tatctaaaaa tagacattga
    11041 aatttttgta ttaatattac ttatctgaat cattaatata aaaagagtta atgctcctaa
    11101 agtatagaaa cattaactct tttttaaatt ttatttattg gtattatata taatttaatt
    11161 attattaatt gtatatattt attctttatt ttttattcct ttttctctct attctggatt
    11221 atacttttat ttattttatt aaggctttag cccccctatc cccccgtcac tcctttcgag
    11281 ttccgagttc catctttcca agttccatta tttatatgtt ttatcttttt tcattttttt
    11341 atttttaagt aaaattagtg acctaaaact tttctattat ctacttattc aatttaaaat
    11401 caacttattt agctcttttt ttgagctttt ttcaatctaa tggctacttt tcttcactat
    11461 ctaaaaaatc tctgttttct gatgtgtttt tattgctttt tgccctattt ctatctatca
    11521 tcatatagct tttttaactt atcttatatt gttacttatt atttactgct tctattgaag
    11581 tagcaacaac tgttaatata gtaccaacta cttttattac cgttataata tccatctatc
    11641 tcacctcctt taaatatgca tagatatatg agtaactatc tctaacagga actaatttga
    11701 ttacatactt agcaatctct aaatctatat atactaatgt ttcattatca tatcccctac
    11761 cattacagaa ccttactaca gtatcaaaac tctttattaa atcaaactga atgtaattaa
    11821 gttctttctg caacttcttt aaatctattt cctttttatc atttaactca attattaaat
    11881 ctctaggatt ccattgatta gcaagccctc catcatagaa atcaaaacgc attactccaa
    11941 gtagttcctt agaactatac tcaaaagact tttctttact tagttctttc atcttattac
    12001 ctcctaatat tcttctgcta acatcatagt tgagtattca ttatcatcta ttacgaaaac
    12061 ttttatgttt ccattaacct ctatattttc taaatctata acatactttt ctttatactc
    12121 tggaacttct tgactatgtt caattacaat tccttcttta cattttctta ctttgaaaac
    12181 ttgtaagtag tctaattcct ttccctcttc attcaactta tcaatcatac tccaaagtat
    12241 aagttgtaat cttaaatcta acttttcatt agctcctctt gttatatatc tgctctcttt
    12301 cttaaacatc ttaatcctcc taaaaataat taaagctacc taattacttt tcttgtaatt
    12361 aagtagctat tttaattctt gcttcaacat ttctatgaat tcatctgaaa atactttctc
    12421 tttaatggac ttctttatta actccattaa ctctaattca gttacatcaa gacttcttgg
    12481 aatataagtc tcatcacctc ttctagtaga tttatataca cagaatctta tttcattcct
    12541 ttttagttcc ttgatatata tcttttctat tgtatatgta tattcatcat ctccaacttt
    12601 tccttgattt attatcttac aatacttagt ttccttgatt atcttatcct cttttatatt
    12661 cttactcaca ttaaattaac ctccatttat tctaatagga acttagaaag ttcttctgtt
    12721 gttactacta caattgcata atcacttgaa agcaaaaata aggaagaagt ccaattaact
    12781 ccttccttac attcaataat atcagtatat tctggtatta aaccttgtaa cttatctttc
    12841 tttaacattt cgataacaac gatattttct gctataacta tatatcctcc taaatctttc
    12901 tctatatctc tattagctcc ataattttca cctaatacat taatgacgtc taaaacatta
    12961 ctgattactt ctataggata tccttttaat tgatttttta aataaacctt tttcattata
    13021 aatttctccc ttctaagaaa gtaaaaaact tttacttgta tacataaata caagtaaaag
    13081 tttgtttttc taagttaata atatatattt aatttatctt ttaatacagt aattaaccta
    13141 attctaatat tgtaatttaa aagtgttttt gaagatttct acaaaaaatt tacgaacctt
    13201 cttgtattgt tcaatcaaat tatcttcatt tttaatccct tatattcatg atattttttt
    13261 atccattatc atagaaataa tatataacac taatactggt gctactaata atgaagtaca
    13321 tactatacca atacttactg aaatatttgt agctatgatt cctataatcg gtccacctaa
    13381 aatttgacct aaggaattca tttgtccatt tatagaaagc acagtagctc tagaattatc
    13441 atctatatgc ccatttaacc acgcactgaa tataggttca tttataattc taaaggtatt
    13501 tgttgccaaa taagctatta acattaaact aaagtttctt gtaagagcaa atatcaacat
    13561 agacgatata taaagtatat ttatgcataa taatagtttt ccatttttcc tattatcctc
    13621 attcttaaga ttctttgcca taaaatgcat tactatgaag ctcaatatca ttcctaaaat
    13681 tccaaaaatt ccaaaccaag tcactgaact aaggtttcca agtttaggaa gtgtagtatc
    13741 ttgtaaaaaa tgcgcattag aaagtctatc ataaccttca cttgataatc cataaaataa
    13801 agttactgca agtaaaatca taattataga tttacttttt acaaatttaa gaccagattt
    13861 aaatgtatat accatctttt tgaatgtatt taaatcccca ggagcagatg gtttaaaatt
    13921 attttctggc atatataacc ataaaaataa tgcaagaatt ataaataaaa ctccactaac
    13981 tataatagga agccttacag agaaattagc tattacagtg cttagtacta ttccaataaa
    14041 tgctcctatc tgccctgctt gtgctccctt tatataaatt tcatctaaat ctttattctt
    14101 ctcttcttcc gcaatccaag cttcaagcga gccactgata aaagtagacc ctaatcccca
    14161 tacaatctgt gctacaagta cgaaaacaaa actagaaata gaaccttcta aaataaatcc
    14221 cactcctgtt aaaactcccc caataacaat agatagttta cgactataca catctgcaac
    14281 tatacctgta ggaatttcaa atataaagca tgctaattcc aaagtagttc caacaagtat
    14341 aagctgaagt ggatttaaat gaactatttc aatgtgatac actatcataa ctgtagctac
    14401 taacgaaaaa cacattgctg taatagctga aaataataaa taagttttat atgctgaaag
    14461 tttattaacc attaatcatc accatcctag tatttcttaa aaaatttgtt tgtttttcat
    14521 aatcttcata tttaaatata taaatcatca aaataatctc cttaaacaaa aaaagctgta
    14581 aatgaacata attcacttac agcctcataa tcatataacc taaatataaa cataaaatat
    14641 agaactttaa ttatataact tatgattaag ctattcaaat taaaatctta taccatatct
    14701 atatatagca aaaatagaca taaaaatacc gtgtttagaa cacggttttc ctatcctgca
    14761 aataaagatg taacatgctc attataatac taattatttt tagagtacac aaataaacat
    14821 ggtaatatca atattatcaa taattgattc taccacaaat aaaccttgag ttattcaaga
    14881 tttttcatat acttttaaaa ataatcctta ttcagttaat ttaattaaaa attacctctt
    14941 tgcgcatatt tacaacacct ttacatttat attttttatg cttatatttt agtataatta
    15001 tagtatatat aattttaaaa attttttcaa tgctacagat tctgaaaaac aaattctaat
    15061 tttctcaagt agtgaaaaat gaagaatcta taaataaaat taaaataccc aacaaaaatt
    15121 caaactttag atttttgtaa gtttcattgc aaaaattaaa gcacacctat cgtccattta
    15181 tatatccaat tctatccaaa acctcattta tttatggtac ggttcgttct tcattattct
    15241 aaaggctcta taaacttgtt ctagtaacat tactctaaat aattgatgtg gaaatgtcat
    15301 ttttgaaaag cataatttgt aatcagctct ttttattact tcttgtgata aaccaaggct
    15361 tcctccaatt acaaaggtaa tatttgaatt tcccataact ccacaattct ctatatactt
    15421 tgaaaatttc tcagaagtta tttcattacc ttttaaatcc atagcaatta catgcatatt
    15481 atcctttatt tttgaaagta ttaacttacc ttctttttct tttatttgaa gctcctcttt
    15541 ttctgaagca ttatctggtg ttttctcatc aggaagttct attatttcta atttacaata
    15601 tctacttaat ctttttgaat actcatctat agcttgcttt aaatactttt cctttagttt
    15661 ccctactgaa ataactgtta tattcatctt tcgccctcct agttttaact accaataata
    15721 ttaccataaa gtaaagctct tagtaatcct ataaaatacc ctatacctct tattgataat
    15781 ttataattag tgagatatat aaatgagtta ttaattttta gtacatggtc ttaataaatt
    15841 ttaaatacta taatatttta aaactaaaat attaattttt atatataaaa aagactatgc
    15901 aagatatatt cttacatagt cttttaaaaa ttttatagca tatgcattgc agctccagta
    15961 aagaatggta ttaaccaaat tacccaaaca attatactaa atttgtggaa tttagctatc
    16021 atatttttat ctttctttac taaaactaca gttgcccaga tagcatgtac taacattaat
    16081 attaatgcta atccacctgt tattgtatgg aaactaaagt ttattcctcc agctatcttt
    16141 tccatagctc cagttcctgt agtatcacaa attagaccta aatagaaaaa tgctaagtgc
    16201 catggtttta aagttttttg aatcttttca gaccaaacac ctatactata aaataatagt
    16261 gctaaattca taaatattat tgctataatt aattcttttg acatcacttt catctccttt
    16321 tttatttgaa ccatgttcat attataatac cattgtattc tacttacaat ttagtaatat
    16381 taatttctta ataaaaaggc tttattaatt ttcaaaattt aataattgca aagcaataca
    16441 tttaataata attatttaca aattatcatt ctttatcaaa ttcagattta taaaaaaaag
    16501 taagaacatt tttacatgtc cttacttgta aattttaata tcctaattct tcatttaaaa
    16561 atattacatg tattctattt gaattacttt gacttctgat taaagtatat tctctacata
    16621 tcttttctgg acttttacaa tccatgcaat ttcccacctt agcacaaggt gttttcttat
    16681 ttaatctttt tgcattagca ggagctgaaa tccttctatt tctttgtata gcctcactta
    16741 aattttcaac aattttatta tatcctgcta caattatgac cttatctggt ccataaagca
    16801 ttgctgctac tctgtttcca ttaccatcaa cattatatat ttcaccctct tcagtaattg
    16861 catttgtact tgtgaaatat acatctgatg aaaaggcacc tctgtatatc ttttttatat
    16921 cttcaggttt taacccctct ttatatctat ctaaaaattc atatcttcca cttctaagat
    16981 gatctataac tccagcttca aataaggaca tagagcctcc gcaagctact atatctccac
    17041 tattagttaa ttcttctatc ttacttataa gttcttcttt tgaatttact aaatagccat
    17101 ttatattatt ttcttctaaa gcttttataa ctttattaat tctacattct cttaaccaca
    17161 taacattctt atccataata taaactcccg ttgccaaaaa tctataaata cctataaatc
    17221 ttgtttaatt ccctgttcta cgagtcagat tttgaaaact atgaatagta ttctactttc
    17281 ctttgatata actcttc
//
LOCUS       DAEVGI010000038        17082 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEVGI010000038 DAEVGI010000000
VERSION     DAEVGI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 17082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17082
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.038"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            201..1313
                     /locus_tag="K8M77_001125"
     CDS             201..1313
                     /locus_tag="K8M77_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7085633.1"
                     /translation="MKKKFLSAVLAVITTATLLAGCGSSNDGQSSSGDTGSKGDKMKV
                     GMVTDAGTIDDKSFNQGTWEGIKKAEKDLGVESKYLKPSGTTEADYLKEIGNLYDAGF
                     KFIVTPGFKFETAIYKGQEKYKDAKFVLLDGAPHSGAKDAKPEVSENTVSIFFAEEQA
                     GFMAGVATALQLKEGQLGFIGGMEFPPVQKFNWGFQQGVAFANEKLGTHMSLEAKNVI
                     YQGSFDDSAAGGQIAAQMYDNGVKAIFCAAGGVGVGAIQEAKNRAASGKEAWIIGVDV
                     DQYDDGIYEGDKSVILTSAVKRIDHASYDMIKKELDGTFPGGQTLTYDASNDGVGLPD
                     KNPNLSEETMTEVNKVFEQLKNGEIKVSNEKGDLIN"
     gene            1446..2981
                     /locus_tag="K8M77_001126"
     CDS             1446..2981
                     /locus_tag="K8M77_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085634.1"
                     /translation="MEYVVEMLNIRKEFPGIVANDDITLQLKKGEIHALLGENGAGKS
                     TLMGILFGMNQPDKGVIKVRGKEVKITNPNVANDLGIGMVHQHFKLVENFTVTQNIVL
                     GCEPKILLGLGMDLNKAAKRIEKLSNQYGLNVDPNAKIENISVGMQQRVEILKMLYRD
                     ADVLILDEPTAVLTPQEIDELIKIMKNLINEGKSIIIITHKLKEIKAAADRCTVIRRG
                     RYIGTVDVKTTSEAEMAKMMVGREVSFKVNKKPAKPGEIVLDIKNLSVKNNKKVLGLK
                     DFSIDVRAGEIVGIAGVEGNGQSELIEAITGLRKSESGTINFKNKDINRESIRNRINS
                     GIAHIPEDRHKRGLVLDYTIEENMVLEVYDKKPFSNKGLLNKKEIKKYAEKIIDEFDV
                     RSGEGAESIARSLSGGNQQKAIIGREIELNPELLIAAQPTRGLDVGSIEYIHKRLVEQ
                     RDRGKAVLLVSLELDEILNVSDRIAIINNGELIGIVNADETNENEVGLMMAGIRGGEK
                     HEV"
     gene            2971..4068
                     /locus_tag="K8M77_001127"
     CDS             2971..4068
                     /locus_tag="K8M77_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085635.1"
                     /translation="MKFKQFIISLMAIILGLIAGAILMVIMGHNPIDGYSFLFQGGLM
                     NIERIGNTLATATPLILTGLSLAFAFRTGLFNIGAPGQMLMGGFFATAVGLTVKLPAP
                     VLIPLMILAGILGGAIWAFIPGALKAKFNVHEVVSTIMMNWIAYWIVYYMVPMYFKGE
                     FLETESAQLPAAATLRIQSLTDFFQGSFINMGILIAIISVFAVWFILNKTVLGYELKA
                     VGFNRDAAEYSGISVNRNIVLSMMIAGALAGLAGVVQYTGNATSIQIGVMPTQGFDGI
                     AVALLGASNPIGVLFSALFFGLLYTGKGFMNAMTSIPPEIADTIMATIIYFSATSIIM
                     ERVLNKFQKRKKDKLNKNTNQETTKERVVSK"
     gene            4068..5021
                     /locus_tag="K8M77_001128"
     CDS             4068..5021
                     /locus_tag="K8M77_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085636.1"
                     /translation="MWETIQQIFPYAIAFTIPLLITALGALFSERSGVVNIGLDGLMI
                     VGSFSGAFTVSKLQEVMPGSSLPLWIGLGVAVLVGILFSLLHAFASINLNADQVISGT
                     AINMIAGALTVFLARNMTGSGNIRIMQGFIPKDVPLLSSIPVIGDLFFSKTYATTWLV
                     ILILAISTFLLYKTSFGLRLRACGEHPQAADAAGINVYKMRYIGVMISGAFSSLGGAI
                     ILVTYGGEFTGSVAGLGFLALAALIFGQWKPWGVLGATLFFGFATTIANVSQIIPSLS
                     TIPPVLLKICPYVVTLIALIAFSKTSQAPKAAGEPYQKDKR"
     gene            5224..5592
                     /locus_tag="K8M77_001129"
     CDS             5224..5592
                     /locus_tag="K8M77_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBI7085637.1"
                     /translation="MLAKDFLDFKNWCVVGDVGNEKKYAYRILNKLKENGYNAFGVSL
                     KEGENIFKSLKEVPCNIEVIDLCINPVKGIEFIKEAKTLGINKVLIQPGAESKEILSF
                     CKKNNICAIEGCALVELSRR"
     gene            5844..7133
                     /locus_tag="K8M77_001130"
     CDS             5844..7133
                     /locus_tag="K8M77_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBI7085638.1"
                     /translation="MEKFFKLKENNTDAKTEFIAGLTTFMTMAYILIVNPSILSATGM
                     DQGAVFTATALSAVIATLIMGLYAKLPFAQAPGMGLNAFFAYTIVIQMGYSFEFALTA
                     VLLEGIIFILLTIFNVREAIVDSIPRGIKNAISVGIGLLISLIGLEGAGIVVHTDGGT
                     IVSLGNIVSGSGLLAIIGLLITSVLIAKNVKGALFIGMIITAIIGIPMGITPMPSKII
                     STPPSIAPTFFKFDFHNIFSLDMVIALFTLLFMDMFDTIGTLVGVATKAKMLDKDGKV
                     PNIKKALFSDAVGTTLGAFLGTSTVSTFVESASGVAEGGRTGLTAVSTAFMFFLALFF
                     APLFAIITPAVTASALVLVGLFMIEPIKEIDLHDFTEAIPAFLTIIMMPFAYSISDGI
                     VFGVISYIILKLFTGKRKEISLTTVILGLVFLLKFLI"
     gene            7280..8197
                     /locus_tag="K8M77_001131"
     CDS             7280..8197
                     /locus_tag="K8M77_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBI7085639.1"
                     /translation="MKVGLVLSGGGGKGAYELGVWKALVEMKLDKYIEVISGTSIGAF
                     NAALFAQGDMDNAQALWDEVTMEKLVPMTKMDLLKKGVALALGVKSINFVKKHMSDRL
                     ENGDFPKDGAYEVVNKFIDINKVKESGKICYAACTELPDCKVKYFKINDYDEEVGREI
                     IMASASLPLIYDSSEVDGKKYLDGGMVDNTPIQPVYGEGCDLIIVVHLSKEGTVDRSL
                     YPNAQIIEIVPKSLDDSMINGTLNLDIDAKRLRTQQGYEDTMNLMSPIMTLAKIRFEF
                     EMNEKNPILYRLFNSFKEIKEKCSKRHYS"
     gene            8593..10929
                     /gene="gshAB"
                     /locus_tag="K8M77_001132"
     CDS             8593..10929
                     /gene="gshAB"
                     /locus_tag="K8M77_001132"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBI7085640.1"
                     /translation="MVNLDNGLLKIIKDESLEDYFIKANFGLEKENVRVTERGNLALT
                     PHPKAFGDRENNPYIKTDFSESQLEMVTPICNTLEEVYSFICNLNKVVSLEIMKNGEF
                     LWPQSNPPILPREEEIPIAKLSNREDELYRENLSYKYGKKKQVISGIHYNFSFKEEFI
                     KLLYKELKVKKDFREFKDDIYLRMARNFQKYHWLLIYLTGASPVFHESYIDEIKEEGE
                     KLGEDSYYIKDDTSLRNSSYGYKNKKDYYVSYNSIEEYASDIKNLVKDKEIQSIKEYY
                     NPIRLKSLGSEDMLESLLNKGIDYLEVRLLDLDPLSVQGVNKETLYLVHLFMIYTLLK
                     ENKEITYKDQEEFFKNHDMVSLKGRNEEAVIHENGIPVLLKDKGREILSEMDEIVEIL
                     FSNNEKFKNVIKRALEKINNPHDTISEKLIKDIKEEGYINFHMRLAKEYLNNFKNKEF
                     NLVGYEDLELSTQILILDAIKRGIEFNMMDRLENFISLSDGEKVEYVKQATKTSKDSY
                     ITALIMENKLVTKDILRENNIRVPKGKDYENIDEAKKDFRLFKDEKIVIKPKSTNFGL
                     GISIFPGEYSREDYDKAVEIAFREDSSILIEEFMTGKEYRFLVIGKEVVGILHREPAN
                     VIGNGESTIEELVSEKNKDPLRGKGYKTPLEKIKLGEIEEMFLKNQGLSFKSIPKNGE
                     KIYLRENSNISTGGDSIDFTDKIHPSYKDVALKSAKAVKALICGVDMVIDDIEEEAKE
                     KNHGIIELNFNPAIHIHCFPYKGENRKAGEKILDLLFN"
     gene            complement(11064..11729)
                     /locus_tag="K8M77_001133"
     CDS             complement(11064..11729)
                     /locus_tag="K8M77_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085641.1"
                     /translation="MNAKEFLDKEKTTYGNLYSELIFVLDDIEDLSEDSTLKERAYYK
                     NIKILKKYNDLLDKLSRELPEKKSFLSFLNKSKENEIIKECEDFKKTYKDSLDKIFVC
                     SKCMCVKCIRECGFNPCLSCNKTGKVNYCDKENTNLIVFKNFIKQQYNNETNENEPIE
                     ILCEVEMLDMDKRFRIIKEVLSGEQLVLEYVYNIKDGDLYNSIEDVDLFNEIIDIYES
                     NKI"
     gene            11877..12566
                     /locus_tag="K8M77_001134"
     CDS             11877..12566
                     /locus_tag="K8M77_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085642.1"
                     /translation="MKKHKRFGQLILFFIAFFFLVGCFNISPIKKVKAETFGIENTIA
                     KGGRSSGGSSRSSGSSSRSSSKSSSYNSSKKYSKPSSSSSEKKKSQGNSNYSTTDKFN
                     KPSSGSNSSSDKVESGKDSSNSDKGSTQGNNNYSTTDKFNSKAPNNQEGSSKENNKYE
                     NNDKSSGSSNKSYRSSNSGGFSFFDMLMLNNILNMFGNMFSFMFIRRPFLGIGTVLII
                     ALIIFAIRKRR"
     gene            12652..13365
                     /locus_tag="K8M77_001135"
     CDS             12652..13365
                     /locus_tag="K8M77_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBI7085643.1"
                     /translation="MGIFNRISNMIKAKTNSALDEMENPVELLDQKIRDMEKSFNEAK
                     RSSAQIFGNLKDTEKKMNEAKEESAQYDEKVRLAMSKGNEELAKKALKLKLDSDKKFE
                     SLKSSYEGQRAKADVLKGKLVELEKELDKTRSYRDEAVARLNNAEASKQINEVIANVQ
                     SKSNSINIDDIERSISRKESYAAGLEELKGESLDDEFAKLEELSLDDELNKYRTQNTQ
                     ETKTSDSEVDLELEKYKNK"
     gene            13661..14491
                     /locus_tag="K8M77_001136"
     CDS             13661..14491
                     /locus_tag="K8M77_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBI7085644.1"
                     /translation="MNYKLVCIDMDGTLLDSHHRVSERNKEALKKAIEKGVHIAISTG
                     RVFPSARIYGNIIGVNAPLICSNGSYIKDKNTGELIFKSTLDRETYFKICDIINKYKF
                     LAYVDSTDGLIADTDIPEDDSHRLMNSWVDEEEKIKFYKYDDLRDAYDEHGEDILKFI
                     IIKKEGGPNIEEAKEEFEALEGVDLVYASWGGCIEIMKKGTSKGSAVKALADKLGISL
                     EEVICIGDSGNDISMLEEAGLSVVMGNAPDFIKEYGDYITDTNENDGVAKAIEKFILE
                     "
     gene            14608..15012
                     /locus_tag="K8M77_001137"
     CDS             14608..15012
                     /locus_tag="K8M77_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 family protein"
                     /protein_id="HBI7085645.1"
                     /translation="MFSFTFDEKEYTLSEEKLLYFFNEDVEGFDIDKLFEILNSSEGI
                     SFGKAYYDGPCEECKFGLEEKKKSFPFLEFNMFIYAKNGKFVISNIEKAYEGLTYNKL
                     LRDKKVDKSYLVNINVCKNCGNFAVEVEELEV"
     gene            15189..15821
                     /locus_tag="K8M77_001138"
     CDS             15189..15821
                     /locus_tag="K8M77_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI7085646.1"
                     /translation="MSLLKKVSKIVVGVTMASLLLVGCGGTGKKAKENIPQDKLPVAT
                     IEVKNFGTIEAELYPNKAPNTVDNFISLANSGFYDGLTIHRIVKGFVLQGGDPKGTGT
                     GGPGYMIKGEFSSNGVENDIKHEEGILSMARSQNPDSAGSQFFIVTKEAPHLDGQYAA
                     FGKVTKGLDVVHEIEKISVGANDKPVEDVVIESIKVDTKGIEYKEPEKVK"
     gene            15963..17042
                     /gene="dinB"
                     /locus_tag="K8M77_001139"
     CDS             15963..17042
                     /gene="dinB"
                     /locus_tag="K8M77_001139"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBI7085647.1"
                     /translation="MKENRKIIHIDMDAFYASIEQRDNPKYKGKPLIVGGDPNRRGVV
                     ATCSYEARKYGIHSAMPSLTAYKLCPKAIFIRPRMEVYKKVSRQVMNILNEYSNLVEP
                     LSLDEAFVDVSKSKRCKGSATLIALEIKERIFKEVGLTASAGVSFNKFLAKMASDFRK
                     PDGITVITEENSKDFIRKLPIGKFFGVGRVTKNKLNNIGVFKGEDLLGFSEKELIGIL
                     GDRGKILYEFARGIDNRQVNPYRIRKSIGKEITLREDIEDIEEMIEILDKIAERVSES
                     LCLLNKKGKTVTLKVKFNDFKHITRSITLEHFLKEKKEIMECVKDLISIVDFKNKKVR
                     LLGITISSLEKNIIIEEREQLSFDV"
BASE COUNT         6738 a         1721 c         3166 g         5457 t
ORIGIN      
        1 aataaagtat taaaataaaa aaaacacata gaaggatagc gctttcacgg tttaatatta
       61 tgttgtaaac tcgaatatta agttaaaaat atcttataaa tattaatttg ttatttttga
      121 ttattaattt aattttagtt tattacataa tattaaaagt aagtattatt ttataggtat
      181 caaggaggaa tattaaaaaa atgaaaaaga aatttctttc agctgtattg gcagtaatta
      241 caacagctac tttacttgca ggttgtggaa gttcaaatga tggacaatca tcatcaggtg
      301 atactggttc taagggagac aaaatgaaag ttggaatggt taccgatgct ggtacaatag
      361 acgataagtc atttaaccaa ggaacttggg aaggaataaa aaaagctgaa aaagacttag
      421 gagtagaatc taagtattta aaaccaagtg gaacaacaga agctgattat ttaaaagaaa
      481 taggaaacct ttacgatgca ggattcaaat ttatagtaac tccaggattc aaatttgaga
      541 cagctatata taaaggacaa gaaaaatata aagatgctaa atttgtatta ttagatggtg
      601 ctcctcattc aggcgctaaa gatgcaaaac cagaagtatc agagaatacg gtttcaatat
      661 tctttgctga agagcaagct ggttttatgg caggtgttgc cacagcatta caacttaaag
      721 aaggtcaatt gggatttata ggaggaatgg aatttccgcc agtacaaaaa tttaattggg
      781 gattccaaca aggggtagcc tttgctaatg aaaaattagg aactcacatg tctcttgaag
      841 cgaaaaatgt tatatatcaa ggatcatttg atgattcagc tgcaggtgga caaatagctg
      901 ctcaaatgta tgacaatgga gtaaaagcta tattctgtgc tgctggtggt gttggtgtag
      961 gcgctataca agaagctaaa aacagagctg cttcaggaaa agaagcttgg ataataggtg
     1021 ttgacgttga ccaatacgat gatggtatat atgaaggaga taaatctgta atacttactt
     1081 cagcagttaa gagaatagat cacgcttctt atgacatgat aaaaaaagaa ttagatggaa
     1141 ctttcccagg tggacaaact ttaacatatg atgcatcaaa tgatggtgta ggtttacctg
     1201 ataagaatcc aaacttaagt gaagaaacta tgactgaagt aaataaagta tttgaacagt
     1261 taaaaaatgg agaaataaaa gtttctaacg aaaaaggaga cttaataaat taatttaaaa
     1321 ttcaaggaag tttttgttaa ggggatggag ctttcatccc cttttattat ctaataaaac
     1381 aaatatatta tataaaaatt acaattataa gtagtataga atttgagtat taaggaggat
     1441 aacttatgga atatgtagta gaaatgctta atatccgtaa agaatttcct ggtatagtag
     1501 caaatgatga tataactttg caacttaaaa aaggagagat acatgcttta cttggagaaa
     1561 atggagctgg taaatctact ttaatgggaa ttctttttgg aatgaaccaa ccagataaag
     1621 gggttataaa ggttagaggt aaagaagtaa aaattactaa tccaaatgtt gcaaatgatc
     1681 ttggaatagg aatggtacac caacatttta aattagttga aaattttaca gtaactcaaa
     1741 atatagtttt aggatgcgag cctaagattc ttttaggatt aggaatggat ttaaacaagg
     1801 cagctaaaag aattgaaaaa ttgtcaaatc aatatggatt aaacgttgac ccaaatgcaa
     1861 agattgaaaa tatatctgtt ggtatgcaac aaagggttga aatattaaaa atgctttata
     1921 gagatgctga tgttcttata ttagatgagc ctactgcagt tttaacccct caagaaattg
     1981 atgaacttat aaaaataatg aaaaatctta taaatgaggg aaaatcaata ataattataa
     2041 ctcataaact taaggagata aaagctgctg cagatagatg tacagttata agaagaggta
     2101 gatacattgg tactgtagat gtaaaaacta ctagcgaagc tgaaatggct aaaatgatgg
     2161 taggaagaga agtatcattt aaagttaata aaaagcctgc taagcctggg gagatagtat
     2221 tagatattaa aaatctttca gttaagaata ataagaaagt attaggatta aaggactttt
     2281 ctattgatgt tagagcagga gaaatagtag gtatagcagg ggtagaaggt aatggtcaaa
     2341 gtgaacttat tgaggctatt acaggactta gaaaaagtga aagtggaact ataaacttca
     2401 aaaacaagga tataaataga gaatctataa gaaatagaat aaactcaggg attgcacata
     2461 ttccagaaga tagacacaag agaggactag ttttagatta tacaattgaa gagaatatgg
     2521 ttctagaagt gtatgataag aaaccttttt caaataaagg tttattaaac aaaaaagaaa
     2581 taaaaaaata tgcagaaaaa ataatagatg aatttgatgt aagatctgga gaaggggctg
     2641 aatcaatagc aagatctctt tcaggaggaa atcagcaaaa agcaattata ggtcgtgaaa
     2701 tagaattaaa tccagaactt ttaatagcag cacaacctac tagaggactt gatgtaggat
     2761 ctatagagta tattcataaa aggcttgtcg agcaaagaga tagaggaaaa gctgtgcttt
     2821 tagtttccct tgaattagat gaaatattaa atgtctcaga tagaattgcc ataataaata
     2881 acggagaact tataggtatt gtaaatgcag atgaaactaa tgaaaatgag gtaggtctta
     2941 tgatggctgg tataagagga ggagaaaagc atgaagttta aacagtttat tatatcttta
     3001 atggctatta tattaggact tattgcaggt gcaattttaa tggttattat gggtcataat
     3061 ccaatagatg gttattcttt cttattccaa ggtggactta tgaacataga aagaatagga
     3121 aatactttag ctacagctac tcctcttata ttaacaggac tttccttagc ttttgctttt
     3181 agaactggat tatttaatat aggggctcca ggacaaatgc ttatgggagg attttttgct
     3241 acagcagtag gattaacagt gaaattacca gctccagtat taattccatt aatgattctt
     3301 gctggaatac ttgggggagc tatatgggca tttataccag gcgctttaaa agctaagttt
     3361 aatgttcatg aggttgtttc aacaataatg atgaactgga tagcttattg gatagtttat
     3421 tatatggttc ctatgtattt taagggagag ttcttagaaa ctgaatcagc acagcttcca
     3481 gcggcagcta ctttaagaat acaatcgctt acagactttt ttcaaggatc atttataaac
     3541 atgggaatat taatagcaat tatttctgta tttgcagttt ggtttatact aaataaaaca
     3601 gttttaggat atgaattaaa ggccgttgga tttaatagag atgctgcaga atattcaggt
     3661 atttcagtaa atagaaacat agtattatca atgatgatag ctggtgcttt agcaggactt
     3721 gcaggtgttg ttcaatatac aggaaacgct acaagcatac aaataggtgt tatgccaaca
     3781 caaggatttg atggcatagc agtagcttta cttggtgcaa gtaatcctat aggagtttta
     3841 ttctcagctt tattctttgg tcttttatat acaggaaaag gatttatgaa tgctatgaca
     3901 agcatacctc cagaaatcgc agatactata atggctacaa taatatattt ctctgctaca
     3961 agtataataa tggagagagt tcttaataaa ttccaaaaaa gaaagaaaga taaattaaat
     4021 aaaaatacta atcaagaaac aactaaagaa agggtggtga gcaaataatg tgggaaacaa
     4081 ttcaacaaat atttccttat gcaatagctt ttactatacc tcttttaata accgcattag
     4141 gtgctttatt ttctgaaaga agcggtgtag taaatatagg acttgatggt cttatgatcg
     4201 taggatcttt tagtggggca tttacagtat caaagcttca agaggtcatg ccaggtagct
     4261 cacttccttt gtggattggt ttaggagttg cagttttagt aggaatatta ttctccttat
     4321 tacatgcatt tgcaagtata aatttaaacg cagatcaggt aataagtggt acagctataa
     4381 atatgatagc tggagcttta actgtattct tagctagaaa tatgacaggg agtggtaata
     4441 taagaatcat gcaaggtttt ataccgaagg atgttccatt attatcttcg ataccagtaa
     4501 taggagattt attcttttca aaaacttatg caactacatg gttagttatt ttaatacttg
     4561 ctataagtac attcttatta tataagacaa gttttggatt aagacttaga gcttgtggag
     4621 aacatcctca agctgctgat gctgcaggta taaatgttta taaaatgaga tatataggtg
     4681 ttatgatatc aggagcattt tcatctcttg gtggagctat aatacttgta acttacggtg
     4741 gagaatttac aggaagtgtt gcaggacttg gattcctagc tttagctgct ttaatctttg
     4801 gtcaatggaa gccatgggga gtattaggag caacattatt ctttgggttt gctacaacta
     4861 tagcaaatgt atctcaaata ataccatcac tttcgactat acctccagta ttacttaaga
     4921 tatgcccata tgtggttact ttaatcgcat taatagcatt ctctaaaaca tctcaagcgc
     4981 ctaaggctgc tggagagcca tatcaaaagg ataagagata gtaaatttta aaaacttatc
     5041 tctggatagt attttattat atccagaggt agtttttttt attttgaaat atatttttat
     5101 agagaattaa tttaaagata agataaaatt gtaaaaacaa gattaattta ttttatatac
     5161 tttatatttt aaagataatt agccataata gagttaaata aagtttaaat ggaggaatga
     5221 attatgttag ctaaagactt tttggatttt aaaaattggt gtgttgttgg tgatgtagga
     5281 aatgaaaaaa agtatgcata tagaatttta aataaactta aagaaaatgg atataatgct
     5341 tttggagtta gtttaaaaga gggagaaaat atatttaaaa gcttaaaaga agttccttgt
     5401 aatattgaag ttatagactt atgtataaat cctgttaagg gtatagaatt tattaaagag
     5461 gctaaaactt taggaataaa taaggtttta attcaaccag gagctgaaag taaagagata
     5521 cttagttttt gtaagaaaaa taacatctgt gctatagaag gatgtgcatt agttgaatta
     5581 agtagaagat aatatttaaa aagttcgcat gtttaataaa aaagtattta tttaatagat
     5641 ttaaattatg aaaatatgtt tttgtacgta tcatatagga ataaaaatta attaatgtac
     5701 gcatttgttt taaaataatt acaaaaaaac ttattaattt cacttttttg acaaaaatat
     5761 tcgtaaaagt attgataaat ataaaaaatc tgataatata ttaaaggtga tttagaaaaa
     5821 caaatgaaag aaagagtgag attatggaaa agttctttaa gcttaaagaa aataacactg
     5881 atgcaaaaac agaatttatt gctggattaa ctacttttat gactatggct tatatactta
     5941 tagtaaatcc atcaatatta tcagcaacag gaatggatca aggagctgta tttacagcaa
     6001 cggctttatc agcagtaata gcaactttaa taatgggact ttatgctaag ttaccatttg
     6061 cacaagctcc aggaatggga ctaaatgcat tttttgctta tacaatagtt attcaaatgg
     6121 gatattcatt tgaatttgct ttaactgcag ttttattaga aggaataata tttatacttt
     6181 taactatatt taacgtacgt gaagcaatag tagactcaat accaagggga ataaaaaatg
     6241 ctatatcagt aggtatagga ttacttattt ctttaatagg attagaggga gcaggaatcg
     6301 tagtacatac agatggtgga actatagttt ctttaggaaa tatagtttca ggatcaggac
     6361 ttttagcaat aataggtctt ttaataacaa gtgttttaat agctaaaaac gttaagggag
     6421 cattatttat aggtatgatt attacagcaa taataggaat acctatggga ataactccta
     6481 tgccaagcaa gattattagt acgccacctt caatagcacc tactttcttc aagttcgatt
     6541 ttcataacat attctcttta gacatggtaa tagcattatt tacattatta ttcatggata
     6601 tgtttgatac aataggaact ttagttggtg ttgcaactaa ggctaaaatg ttagataagg
     6661 atggaaaagt acctaacata aagaaagctt tattttctga cgcagtaggt acaacattag
     6721 gagctttttt aggaacaagt acagtaagta cttttgtaga gagtgcatca ggggttgcag
     6781 aaggaggaag aactggatta acagcagttt caactgcgtt tatgtttttc ttagctttat
     6841 tctttgctcc attatttgca attataactc cagcagttac agcgtcagct ttagttttag
     6901 ttggattatt tatgatagaa ccaataaaag aaatagactt acatgatttt acagaagcta
     6961 taccagcttt cttaacaata atcatgatgc catttgctta ctcaatatca gatggtatag
     7021 tatttggagt tatatcatac ataatattaa aattattcac tggaaaaaga aaagagataa
     7081 gtttaactac tgttatctta ggattagtat ttttacttaa gtttttaata taacaattaa
     7141 gaaaatagac tatgtgattt atcatatagt ctatttttgt ataatttatt atcaattttt
     7201 tgcataatgt agtaaaataa tataatagat atcttattgg tataaaacga ttcatttaat
     7261 aatgaatgga ggaaatgtta tgaaagttgg attagtttta tcaggtgggg gaggaaaagg
     7321 agcatatgaa ctaggtgttt ggaaagcttt agttgaaatg aaattggaca agtacataga
     7381 agttatatca ggaacttcta ttggtgcatt taatgctgcc ttatttgctc aaggggatat
     7441 ggacaatgct caagccttat gggatgaagt tactatggag aaattagttc ctatgactaa
     7501 aatggatttg ttgaaaaaag gtgtagcttt agccttagga gtaaaaagta ttaactttgt
     7561 taaaaaacat atgtcagata gattagaaaa tggagatttt cctaaggatg gagcttatga
     7621 agttgtaaat aagtttatag atataaataa ggttaaagaa tcagggaaaa tttgttatgc
     7681 tgcctgtacg gaattaccag attgtaaggt taaatatttt aaaattaatg attatgatga
     7741 agaggtagga agagaaataa taatggcctc agcatcactt cctttaatat atgatagctc
     7801 agaggtagat ggtaagaagt atttagatgg aggaatggtt gacaatacac caattcaacc
     7861 tgtttatgga gaaggttgtg atttaattat tgtagtacat ttatctaaag agggaactgt
     7921 agacagaagc ctttatccaa atgctcagat aatagaaata gtgcctaaga gtttagatga
     7981 tagtatgata aatggaacat taaatttaga tatagatgca aaaagattaa gaacacagca
     8041 aggttatgag gatactatga atcttatgag tcctataatg actttagcga aaattagatt
     8101 tgaatttgaa atgaatgaaa aaaatccaat attatatagg ttgtttaata gttttaaaga
     8161 aattaaagaa aaatgttcta aaagacacta tagttaaaaa gctatagtgt ttttttattt
     8221 ataaaataaa aattaaagaa aaaggtcact atagaaataa cagactttta attataaaaa
     8281 taaaacgcat taaccaatag agatttttag agtgtttaga aatatggaga aaaagaaagt
     8341 aattttaaaa aaataaaaga aataaataca ataacacctt tgactatata aggaatttct
     8401 tatatattta taataaagtc tagaattcct ataagaatta aggtgattaa taaatattat
     8461 attattttta tcgattaata taatttaaaa gaaaaatagg ttagttcata aataatacat
     8521 aagtagatgt tttattataa atggttaaat agaactggtt agtatagata agagaattcc
     8581 aggaggagag aaatggtgaa tttagataac ggtttattaa aaattataaa agatgaaagt
     8641 ttagaagatt actttataaa ggctaatttt ggtttagaaa aagaaaatgt tagggttaca
     8701 gaaaggggaa acttagcttt aacacctcat ccaaaggcct ttggagatag ggaaaataat
     8761 ccatatataa aaacagattt ttctgagagt cagttagaaa tggtaacacc tatctgtaat
     8821 actttagagg aagtttatag ttttatatgt aatttaaata aggttgtatc tttagagatt
     8881 atgaaaaatg gagagttttt atggccacag agtaatcctc ctatattacc aagggaagag
     8941 gagattccaa ttgctaagct ttctaataga gaagatgaat tatatagaga aaatcttagt
     9001 tataaatatg gcaaaaagaa acaagttata agtggaatac attataattt ttcatttaaa
     9061 gaggaattta ttaagttact ttataaagaa cttaaagtga aaaaggattt tagagaattt
     9121 aaggatgata tttatcttag aatggctaga aactttcaaa aatatcattg gttattaata
     9181 tacttaactg gagcaagtcc tgttttccat gaaagttata tagatgagat taaagaagag
     9241 ggtgaaaaat taggagagga ttcttattac ataaaagatg atacttcttt aagaaatagt
     9301 tcttatggat ataaaaataa aaaggactat tatgtttcat ataacagtat agaagaatat
     9361 gctagtgaca taaagaattt agttaaggat aaggaaatac aaagtataaa agaatattat
     9421 aaccctataa ggttaaaatc actaggaagc gaagatatgc ttgaaagctt gcttaataag
     9481 gggattgatt atttagaggt aagactttta gacttagatc ctttaagtgt tcaaggagtg
     9541 aataaagaaa cactttatct agtgcactta tttatgattt atactttatt aaaagagaac
     9601 aaggaaataa catataaaga tcaagaagaa tttttcaaaa atcatgatat ggtttcttta
     9661 aagggcagaa atgaagaagc tgttatacat gaaaatggaa ttcctgtttt attaaaagac
     9721 aaaggaagag aaatactaag tgaaatggat gaaattgtag agatcttatt ttcaaataat
     9781 gagaaattta aaaatgttat taaaagagca ttagaaaaaa ttaataatcc tcatgataca
     9841 atttcagaga aacttattaa agatataaaa gaagagggat atattaattt ccatatgaga
     9901 ttagctaagg agtacttaaa taactttaaa aataaagaat ttaatttagt tggttatgaa
     9961 gatttagaat tatcaacaca aatattaata ttagatgcta ttaaaagagg aatagagttt
    10021 aatatgatgg atagattaga gaactttatt tctcttagtg atggagaaaa ggttgaatat
    10081 gttaagcaag caacaaaaac ttctaaggat tcatatataa ctgcattaat aatggaaaat
    10141 aaattagtta ctaaggatat tttaagggaa aataatataa gggttcctaa ggggaaagat
    10201 tatgagaata tagatgaggc aaagaaagat tttagattat ttaaagatga gaaaatagtt
    10261 ataaaaccta agtctactaa ttttggttta ggaattagta tatttcctgg agaatattca
    10321 agggaagact atgataaagc tgttgaaata gcttttagag aggatagttc aatccttata
    10381 gaagagttta tgacaggaaa ggaatataga tttctcgtaa tagggaaaga ggtagtagga
    10441 atacttcata gagaacctgc taatgtaatt ggtaatggag aaagtaccat agaagagctt
    10501 gtttctgaaa agaataaaga tccattaaga ggaaagggat ataagactcc tttagaaaaa
    10561 ataaaattag gagagataga ggaaatgttt ttgaaaaatc aaggactaag ctttaagtct
    10621 attcctaaaa atggagaaaa aatctattta agagagaact ctaatataag cacaggagga
    10681 gatagtatag actttactga caaaatacat cctagttata aagatgtagc attaaagtct
    10741 gctaaggctg ttaaagccct tatatgcgga gtagatatgg taatagatga tatagaggaa
    10801 gaggcgaagg aaaaaaatca tggcataata gaattgaatt ttaacccagc aatacatatt
    10861 cattgtttcc cttataaagg agagaataga aaagctggtg aaaaaatatt agatttattg
    10921 tttaattaaa ttagatataa aacttgtaga aataagatat taaataaaaa gtagatataa
    10981 aataaaattt taagggctgt atcaaaatat aaaaggttat tttaatacag cccttattaa
    11041 gtatttaaat aatttatttt tatttatatc ttattacttt cataaatatc tattatttca
    11101 ttaaaaaggt ctacatcttc aattgaatta tatagatcac catcttttat attgtagaca
    11161 tactcaagta caagttgttc accacttaaa acttctttaa ttattctaaa acgcttgtcc
    11221 atatctaaca tttccacttc acaaagaatt tcaataggtt cattttcatt agtttcatta
    11281 ttgtattgtt gttttataaa gtttttaaat actataagat tagtattttc tttatcacaa
    11341 taattaacct ttccagtttt gttacatgat aaacatgggt taaagccaca ttctcttatg
    11401 cattttacac acatgcattt agaacataca aatattttat caagagaatc tttataagtt
    11461 tttttgaaat cttcacattc ttttataatt tcattttctt tagatttatt taaaaatgac
    11521 aggaaagatt ttttttctgg aagttctcta gataacttat ctaataaatc attatatttt
    11581 tttaatattt taatgttttt gtagtatgct ctttctttta aagtagaatc ttcgcttaaa
    11641 tcttcaatat catctaaaac aaaaattagc tcactgtaaa gattaccata agtagttttt
    11701 tctttatcta aaaattcctt tgcattcatt tgctatactc cttcaccata taaataatat
    11761 atatatttat gatatcatat attatcaaat aattatgaat ttagactatt aaaatataca
    11821 aaatttaata attaatttat aatgatgata tataatacat ttaaggaggg gaagaggtga
    11881 aaaaacataa gaggtttggt cagttaatcc tattttttat agcttttttc tttttagttg
    11941 gatgttttaa tatttctcca attaaaaaag ttaaggcaga aacctttggt atagaaaata
    12001 ctattgctaa aggtggaaga tcctcaggtg ggagctcaag aagttcagga agttcttcta
    12061 gaagttcaag taagagtagt tcttataatt ctagtaaaaa gtattctaag cctagtagtt
    12121 catctagtga aaagaaaaaa agccaaggaa atagtaatta ttccactacg gataagttta
    12181 ataaaccttc atcaggaagc aattcaagtt ctgataaagt agaaagtgga aaagattcta
    12241 gtaactcaga taaaggtagt acacagggaa ataataatta ttcaactaca gataagttta
    12301 atagcaaagc ccctaataat caagaaggaa gttctaaaga aaataataag tatgaaaata
    12361 atgataagtc tagtggtagt tcaaataaaa gctatagaag tagcaattca ggaggattta
    12421 gcttttttga tatgcttatg ttaaataata tacttaatat gtttggaaat atgtttagtt
    12481 ttatgttcat aagaagacct ttcttaggta ttggaactgt tttaataata gctttaataa
    12541 tatttgcaat tagaaaaaga agatagttaa aattaataat tatgacatga atttgtaaaa
    12601 aagagatagt ataataaatc taaataaaaa gtaggtaaag gtggtaataa aatgggaata
    12661 tttaatagaa tatcaaatat gattaaggct aagactaact cagctttaga tgaaatggag
    12721 aatccagtag aattattaga tcaaaaaata agagatatgg agaaatcttt taatgaagct
    12781 aaaagatcat cagctcaaat ttttggtaac ttaaaagata ctgaaaagaa aatgaatgaa
    12841 gctaaggaag agtcagctca gtatgatgaa aaagtaagat tagcaatgag caaaggaaat
    12901 gaagagttag ctaagaaagc tttaaaactt aaattagata gtgataagaa atttgagtct
    12961 ttaaagtcaa gctatgaagg acaaagagct aaagctgatg ttttaaaagg aaaattagtg
    13021 gaattagaaa aagaattaga taaaacaaga agttatagag atgaagcagt agctagactt
    13081 aataatgctg aagcttcaaa acaaataaat gaagttattg caaatgttca aagtaagagt
    13141 aattcaatta atattgatga tatagaaaga agtatttcaa gaaaagagag ttatgctgct
    13201 ggattagaag aattaaaagg tgaaagttta gatgatgaat ttgctaaatt agaagaatta
    13261 agtttagatg atgaattaaa taaatataga actcaaaata cacaagaaac taaaacttca
    13321 gattcagagg tagatttaga attagagaaa tataaaaata aataatatga attttaaagg
    13381 gttatactaa aatagctcgt aaataaaact tataaatatt ggcaagagaa tatttgaatg
    13441 aacttaaatt tttagtgaaa ttcagagatt ctcagaagat atttataagt tttttattta
    13501 agaagagaat tttataagtt tttagaaaaa tattatatag gatttataac aaatagaatt
    13561 ttaagttaaa acatagctac ttttatttaa tggatatata ttaaatcttg attaaaaaag
    13621 aaagtctaca taaaatttaa tttagaaagg ggagaattaa atgaattata aattagtttg
    13681 tatagatatg gatggtacgc ttttagattc tcatcacaga gttagtgaaa gaaataaaga
    13741 ggctttaaag aaagccattg aaaaaggagt acatattgcc atatcaacag gaagggtttt
    13801 tccgtcagct agaatatatg ggaatataat aggagtaaat gcaccattaa tttgttcaaa
    13861 tggttcatat ataaaggata aaaatactgg tgagttaata tttaaatcta cactagacag
    13921 ggaaacttac tttaaaatat gtgatattat aaacaaatat aagtttttag cttatgtaga
    13981 ctctacagat ggactaatag cagatacaga tattccagag gatgatagtc atagacttat
    14041 gaatagctgg gtagatgagg aagaaaaaat aaaattctac aaatatgatg acttaagaga
    14101 tgcttatgat gaacatggag aagatatatt aaagttcata ataataaaaa aagaaggtgg
    14161 acctaatata gaagaagcta aggaggaatt tgaagcctta gaaggagtag atttagttta
    14221 tgcttcttgg ggtggatgta tagagatcat gaaaaaagga acttcaaagg gaagtgcagt
    14281 taaagccctt gcagataaat taggaataag cttagaagaa gtgatatgca ttggtgatag
    14341 tggaaatgat atatctatgc tagaagaggc aggattatct gtagtaatgg gaaatgcacc
    14401 agactttatt aaggaatatg gagattatat aacagataca aatgaaaatg atggcgttgc
    14461 taaggctata gagaagttta tattagagta atataaataa agagtttaat aaaagagaaa
    14521 tttagtttga catattgtct ttaaagttat taaatatgtt tagagataat aataaaatgt
    14581 gaattgttca tagaaaggga tgattaaatg tttagtttta cttttgatga aaaagaatat
    14641 actttatcag aggaaaaatt gctttatttc tttaatgaag atgttgaagg ttttgatata
    14701 gataagcttt ttgaaatatt aaatagtagt gaggggatta gctttggaaa agcttattat
    14761 gatggccctt gtgaagagtg taaatttgga ttagaggaaa aaaagaagag cttcccattt
    14821 ttagagttta atatgtttat ttatgctaag aatggaaagt ttgtaataag caatatagaa
    14881 aaagcttatg aaggtcttac ttacaataaa ttattaagag ataaaaaagt agacaagagc
    14941 tatttagtta atataaatgt ttgtaaaaat tgtgggaact tcgcagttga agttgaagaa
    15001 cttgaagtat aataaaaaag tttttggtat gtattcttat aatacaaaaa agaatacata
    15061 ccttttttgt tttaggaaaa tttaattata tagaaacaaa ttaatagctt atatgacata
    15121 aatttaaagt gcttagatga tataatttaa aacatattag caataaatta taagaaaggg
    15181 gaataaaaat gagtttatta aagaaagtta gtaaaatagt agtaggagtt acaatggcat
    15241 ctttacttct tgtaggatgt ggagggacag gtaaaaaggc aaaggaaaat ataccacagg
    15301 ataaattacc tgtagctact attgaagtta agaattttgg aactatagaa gcagagttat
    15361 atccaaacaa agctccaaat accgttgata actttataag cttagctaat agtggatttt
    15421 atgatggctt aactattcat agaatagtta aagggtttgt gcttcaaggt ggagatccaa
    15481 agggaacagg tacaggtgga ccaggatata tgataaaggg tgaattttct tctaatggag
    15541 tagaaaatga tattaagcat gaagagggaa tattatcaat ggcaaggtct caaaatcctg
    15601 atagtgcagg aagtcaattt tttattgtta caaaagaagc accacattta gatggtcaat
    15661 atgcagcctt tggaaaagtt actaagggat tagatgtggt tcatgaaata gaaaagattt
    15721 cagtaggagc aaatgataaa ccagttgagg atgttgttat agaatcaatt aaggtagata
    15781 caaaaggaat agagtataag gagcctgaaa aggttaaata aagggatttt aaattactaa
    15841 ttataatatt ttaagataag ttttaaatag atttagtgtt taaaacttat cttttataat
    15901 attggaattt aaaaagtctt agtgtaaaat taaaaatgaa catatgtttg catggaggct
    15961 ttatgaaaga gaatagaaag ataattcata tagatatgga cgctttttat gcatccattg
    16021 aacaaagaga taatccaaag tataaaggaa agccgttgat tgttggcggg gatcctaata
    16081 ggagaggggt agttgcaact tgctcttatg aagcaaggaa atacggaata cattcagcta
    16141 tgccttcttt aactgcatat aaattatgtc ctaaagctat ttttataaga cctagaatgg
    16201 aggtttataa aaaagtttct agacaggtta tgaatatatt aaatgaatac tctaatcttg
    16261 tagaaccctt gtccttagat gaggcttttg ttgatgtaag taaaagcaaa aggtgcaaag
    16321 ggtctgcaac cttaatagcc cttgaaataa aggagagaat atttaaagaa gtaggtctta
    16381 ctgcttcagc aggagtttct tttaataaat ttttagcaaa gatggcatca gattttagaa
    16441 aaccagatgg tataacagta ataacagaag agaattcaaa ggattttata agaaagctac
    16501 ctataggaaa attttttgga gttggaagag taactaaaaa taaattaaat aatatagggg
    16561 tatttaaggg ggaagattta cttggatttt ctgaaaaaga gcttataggt attttgggtg
    16621 acaggggaaa gatattatat gaatttgcta gggggataga taatagacaa gtaaatccat
    16681 acagaattag aaagtctata ggtaaagaaa ttaccttaag agaagacata gaagatatag
    16741 aggaaatgat agagatttta gataaaatag cagaaagagt tagtgagagt ttatgtttat
    16801 taaataaaaa aggaaagact gtaaccttaa aggtgaagtt taatgatttt aaacatataa
    16861 caagaagtat tactttagag cactttttaa aagaaaaaaa ggaaataatg gagtgcgtaa
    16921 aagatcttat aagtatagtt gattttaaaa ataaaaaggt aagattatta ggaataacaa
    16981 tatcatcttt agagaaaaat ataataatag aagaaagaga gcagttaagc tttgatgttt
    17041 agacaaatta ttaactatat gaaaacaaat ttcgatatta ta
//
LOCUS       DAEVGI010000039        16909 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEVGI010000039 DAEVGI010000000
VERSION     DAEVGI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 16909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16909
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.039"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            65..529
                     /locus_tag="K8M77_001140"
     CDS             65..529
                     /locus_tag="K8M77_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI7085648.1"
                     /translation="MKESELVLNNILVKLFNNILRIEEFALKSAPFNDLSITEMHTIE
                     AIGIERARTMSEVALDLKITVGTLTTAINKLIKKGYVNRRRIEEDRRVVMIELTEKGT
                     LAYKVHEKFHEEMIEHVLEELGINEEEVLISSLGKLDKFFQKKLELMKSKEN"
     gene            531..1505
                     /locus_tag="K8M77_001141"
     CDS             531..1505
                     /locus_tag="K8M77_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBI7085649.1"
                     /translation="MENAKIIGFGLYTPKNLVENERLQEFLETSDEWIRSRTGIERRY
                     ISLDENTSDLAVEASKKALNQAGLSAEEIDLIIVATVTPDNFTPSTACIVQDKLGAKN
                     AWAFDINAACTGFIYALKLGRSLIRSGEAKNALIIGAETLSKALNWEDRGSCVLFGDG
                     AGAIVLTSTEEDCGIKCVNVKSDGSKGDSLVIQGLPLNSPFKDGREVSKNYINMNGRE
                     IFKFATKVMEESIVEILEKENIKIEDISAIIPHQANLRIIDYVVKRLGIPREKFVTNL
                     QNYGNTSGASIPIALCESINEGNLKKGDNIIMVGFGGGLTWGAALIKL"
     gene            1602..2546
                     /gene="fabD"
                     /locus_tag="K8M77_001142"
     CDS             1602..2546
                     /gene="fabD"
                     /locus_tag="K8M77_001142"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBI7085650.1"
                     /translation="MAKLGFLFAGQGAQYVGMGKEFFDNFEESKEVFKRSSEALGMDM
                     EELCFSDPEGLLNKTEFTQPAIITTNMAILKALDKLGVKSHISCGLSLGEYSALIHSC
                     AINFEDGVKLVKKRGKFMQEAVAEGIGGMVAVLRMTPEQVDEIIEKSSSYGIVEGANY
                     NSPGQIVISGELVALEKAMEFIKEVGGRAIKLPVSAPFHCSMLQPAAEKLEDELNKIS
                     INKINGIVMSNVKGEAYLEEDNIIELLTSQVKKPVLFINDIEKMIESGVDTFIEIGPG
                     KALSGFVKKINKNVTVLNVEDLKSLEKTLSKLREMEVL"
     gene            2550..3290
                     /gene="fabG"
                     /locus_tag="K8M77_001143"
     CDS             2550..3290
                     /gene="fabG"
                     /locus_tag="K8M77_001143"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBI7085651.1"
                     /translation="MLKDKVAIVTGGTRGIGRAIALKLADNGANIVINYRNSDKEAEE
                     LKAILEEKGVKVLTVKCDISNFEDSKNLMDKCKEVFGKIDILVNNAGITKDTLIMRMK
                     EEDFDSVIDVNLKGTFNCAKHASAIMLKQRFGKIINMTSVVGIAGNVGQVNYAASKAG
                     VIGLTKSLAKELGSRGITVNAVAPGFINTDMTASLSEKVKEEASKNIPLKRLGDPEDV
                     ANLVGFLASDAANYITGQVINVDGGMVM"
     gene            3305..4546
                     /gene="fabF"
                     /locus_tag="K8M77_001144"
     CDS             3305..4546
                     /gene="fabF"
                     /locus_tag="K8M77_001144"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBI7085652.1"
                     /translation="MENRVVITGLGAITPIGNNVNEFWTNAKKGVCGIDEITLFDTSD
                     YKVKIAAEVKNFKAEEFIDKKEAKRLDRFTHFALVATEEAMKDAKLDLKDENVDPNRV
                     GVIVGSGIGGYGTLESQFDVLLTKGPNRVSPFAIPSGIINLVAGNIAIKYGAKGVCES
                     LVTACATGVSSIGAAYRSIKHGYSDIVISGGSEASITRAAIAGFTNLKALNINNDPKR
                     ASIPFDKERSGFVMGEGAGILILESLESAKKRGARIYGEIIGYGATCDAYHITSPDPE
                     GDGAKRAIEAAIEEGKINKDEISYINAHGTSTPLNDKFETLAIKGCFGEKAYDIPVSS
                     TKSMTGHLLGAAGAIESVICVKALEEGFIPPTIGYKVPDEDCDLDYVVNEGREAELNY
                     VINNALGFGGHNASLLFKKWD"
     gene            4558..5049
                     /gene="accB"
                     /locus_tag="K8M77_001145"
     CDS             4558..5049
                     /gene="accB"
                     /locus_tag="K8M77_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBI7085653.1"
                     /translation="MNLNEIKELIDKIDSSNIAYFEVKVDNSYIKMDKSLTRNLVEEK
                     KASNIEETEKTDISEEKNESISKVEEVKLQPKEERVQDEDLYLVTSPMVGTFYESPGV
                     GEKSYVSIGSKISSGDVLCIVEAMKLMNEIESEVSGEIIDILVQNGNMVEYGQPLFKV
                     RRA"
     gene            5052..5471
                     /gene="fabZ"
                     /locus_tag="K8M77_001146"
     CDS             5052..5471
                     /gene="fabZ"
                     /locus_tag="K8M77_001146"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBI7085654.1"
                     /translation="MMNINEIKEILPHRYPFLLVDKVEEITESKVVAYKNVTINEPFF
                     QGHFPDYPVMPGVLIVEALAQAGAIALLNKEEFKGKTPFFAGIDKVRFKKQVLPGDTL
                     RLEVEIIKLRGSIGFGKATATVDGKIACSGEIMFAIG"
     gene            5483..6835
                     /locus_tag="K8M77_001147"
     CDS             5483..6835
                     /locus_tag="K8M77_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBI7085655.1"
                     /translation="MFKKILIANRGEIAVRIIRACRELGILTVAVYSEIDKDALHVQL
                     ADEAVCIGPWNSRESYLNIQNILSACVLTGAEAIHPGFGFLSENPRFAKMCEECNIEF
                     IGPNWRAIELMGNKAMAREIMKKADVPVVPGYEGEIESPEFALKIAKKIGFPVIVKAS
                     SGGGGKGIRIVYKEEDFISNYNTAKSEAQANFGDDKIYIERFVENPKHIEVQLLCDKH
                     GNALHLFERDCSAQRKNQKVLEEAPSSILSGEQRDKLGKIAVKAAKAVNYSNVGTIEF
                     LLDKNGEFYFMEMNTRIQVEHPITEMITGVDLVKEQIKVAFGKELEITQDDLKINGHS
                     IECRINAEDPSKNFMPCPGLIKEINFPGGFGVRLDTAIYSGYKIPHCYDSMIGKLIVH
                     GKDREEAISKMKRALDEFAIDGITTNIDYHFLILDHEDFKNGTYNTSFLSEKLVNKYD
                     "
     gene            6828..7706
                     /locus_tag="K8M77_001148"
     CDS             6828..7706
                     /locus_tag="K8M77_001148"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBI7085656.1"
                     /translation="MIKNLLNKRKYITVSSVELNDTELSEDEKPNIPSGMWSKCEKCA
                     KILYTEDLRENFNVCPNCGHHFKLGAYERIKYLTDENTFVEFDKKIIGRNPLDFNGYE
                     EKIKGYQKKSHVIEGVVTGEAYIAQRKVVLCVMDSNFMMGSMGTAVGEKITRAIEYAT
                     KNRLPLIIFTCSGGARMQEGIYSLMQMAKVSGAIYKHGRENLLYITVLTNPTTGGVTA
                     SFAMEGDIILSEPGCLVGFAGRRVIEGTINEKLPDDFQTAEFLLEKGFIDKIVQRKDL
                     KQVITSLLRMHEVDYE"
     gene            7699..8514
                     /locus_tag="K8M77_001149"
     CDS             7699..8514
                     /locus_tag="K8M77_001149"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBI7085657.1"
                     /translation="MSRELIRTADAWNKVKIARDPNRPNAKFYINEIFDEFIELHGDR
                     NFGDDKAIIGGIALLNNLSFTVVGICKGENTKENIKRNFGMPHPEGYRKALRLMKQAE
                     KFKRPVICFVDTPGAFCGIGAEERGQGQAIAQNLVELMGLKVPLISIVIGEGGSGGAL
                     ALAVADKVFMLEHSIYSVLSPEGFASILWKDSSRAEEAASVMKITAQDLKSFNIIDKI
                     IKEPRGGAHKNPIKMAQNIKKTILEALGEMKGTDLDTLLNERYNKYRNIENNL"
     gene            complement(8614..9003)
                     /locus_tag="K8M77_001150"
     CDS             complement(8614..9003)
                     /locus_tag="K8M77_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBI7085658.1"
                     /translation="MDRRTRMVAPRNIINAKQLYSFYVLKELSKGHEVFGNKVLEAFR
                     DKFSSAPLPFPVSSSTIYDTLYDLEQKGLVTSYWIGDDFINKRTKKMYRITDEGKEYY
                     KLHVADYVDALRKNKSIIDILIKMLTN"
     gene            9129..9350
                     /locus_tag="K8M77_001151"
     CDS             9129..9350
                     /locus_tag="K8M77_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085659.1"
                     /translation="MSRKCCCSCNSCRRNSCGCRCGNNCCGFNNCSGFGNCGGFNSCG
                     GFGNCGGFPLLWLLLLGCGGFGNCGGNWF"
     gene            complement(9408..10226)
                     /locus_tag="K8M77_001152"
     CDS             complement(9408..10226)
                     /locus_tag="K8M77_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014521500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBI7085660.1"
                     /translation="MEKLLFGISGLPIGEEGQKFNYSTGIEYLKKIGLEAMELPFVRS
                     VNVTAKNREKIIEAKEKNDFYLSAHGSYFINLNAEEEEKKEKSIERILKGAEALKSVG
                     GRSLVFHPGFYLKASKEEALKEIKKNLKKLPYEGVDYRLETTGKGTQFGNLQELVDIC
                     KEVPSCKLCIDFSHIHARGIGALKEYKDFAEILEYIKDNLGNEALEDLHIHMSGIHYT
                     DKGERNHLPFLESDFNFIECLRALKDYKVKGCIICESPLLEKDALLLKETYEKL"
     gene            10443..11153
                     /locus_tag="K8M77_001153"
     CDS             10443..11153
                     /locus_tag="K8M77_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBI7085661.1"
                     /translation="MEERLQKYMARCGVASRRKCEEIICSGKVKVNDTVVDSLGTKVD
                     PSVDVVSVNNKVISPEENKVYIMLNKPEGYVTTNKDEKNRKTILDIVKVNERIYPIGR
                     LDYDSSGLLLLTNDGDVYNKIIHPRVNLNKEYIALVKGIFTEEEMNKFRKGVDIGDYK
                     TAECEIELLEKYTNSCKVKIIIHEGKNRQIRRMCAAFNHEVVSLKREAIGSLTLKNLK
                     KGEWRYLSSSEENYIKSL"
     gene            11159..11710
                     /locus_tag="K8M77_001154"
     CDS             11159..11710
                     /locus_tag="K8M77_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI7085662.1"
                     /translation="MFKYVSDVSNIYHNIITSFIENKNIAIDATLGNGHDCDFLSELF
                     KKVYAFDIQEEAVSSYKSKNKENVEVILDSHENFETYIKEENVDCIVYNLGYLPGNNK
                     EVTTVAHSTLKSIEIGLDLLGDNGLMIIALYSGHEEGKKEKDAVLQFTSKLPKNKYGV
                     LHTEFTNRAKSAPSLVVIEKKFK"
     gene            11743..12618
                     /locus_tag="K8M77_001155"
     CDS             11743..12618
                     /locus_tag="K8M77_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBI7085663.1"
                     /translation="MESQDLMRLIQVKFPKLSKGQKLIAEYILKNYDKAAFMTAAKLG
                     NNVGVSESTVVRFATELGFSGYPKLQKALQELIKNKLTSVQRLELSNDFISEENALKG
                     VLKADIENIRATLEKINHKTFDEVVNEIFKAKRIYIIGLRSSTALAEFLGFYLNLILD
                     NVRVISNGISDTFEQMLNLGEGDLVIGIGFPRYALKTIDALEFAKTRNAKIVSITDSL
                     ISPLASQSDHTLIAQSNMASFVDSLVAPLSVINALIIAVGIREKEKITSTFSELEKIW
                     KDYNVYSYNKNNIEP"
     gene            12835..14055
                     /locus_tag="K8M77_001156"
     CDS             12835..14055
                     /locus_tag="K8M77_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI7085664.1"
                     /translation="MAKVIVIGAGPAGMMAAISAAENHEVILLEGNERIGKKLFITGK
                     GRCNVTNAKDISEFFDFIPGNPHFLYSALYTYTNIDVMNFFENAGVKLKVERGSRVFP
                     NSDKSSDIISGLSRGLNEALVDLRLHSKVKDVIFNNNKIEAVILENGSKVKGDYFIIT
                     TGGKSYPLTGSTGIGFDLAKKMGHTIVEPKPSLVPIEIEESWVRELQGLSLRNIELKI
                     KNKKNSKVVYSGQGEMLFTHFGISGPLVLSGSRFIKDGEKFEISLDLKPALEEKQLDL
                     RIQKDFKKNLNKDFKNSLDELLPKKLIPVIIELSKIDENKKVNSITKEERRTLLNLLK
                     NLTFTVKGLRDISEAIVTAGGVSTKEIDPSTMQSKIVDNLYFAGEVIDVDAFTGGYNV
                     QIALSTGYLAGKSI"
     gene            14177..14830
                     /locus_tag="K8M77_001157"
     CDS             14177..14830
                     /locus_tag="K8M77_001157"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBI7085665.1"
                     /translation="MNKLITVAIDGPAGAGKSTIAKIIGEKFNLMYINTGSMYRAVTL
                     KALENNISAEEVDKLLVMIDGMDMHFENDELILNGENINSLITMPNISKNVSAYASIR
                     EVRERLVNLMRKMALKYSVIMDGRDIGTVVLKDANFKFFLTASPEERADRRYKELMGK
                     GVEVNYDEILQDIIKRDYLDSNREVDPLRKAEDAIEIDTTGIGIMGVVEKISSYMEK"
     gene            14832..15680
                     /locus_tag="K8M77_001158"
     CDS             14832..15680
                     /locus_tag="K8M77_001158"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBI7085666.1"
                     /translation="MERNVILAKNAGFCFGVKRAVDEAIKYQKEFGKKIYTLGPLIHN
                     NDVVNYLEDNDIFAIELSDTNSLNKGDVVLIRSHGVKESVIKDLTDKGLIVKNATCPY
                     VTNIQLKVKKCYEQGYKIIIVGDENHPEVIGINGWCNDSAIITNGKTELENIPAKVCV
                     VSQTTEKKETWNKVLNEIVKESKEIVAFNTICSATDVRQKSVQELSKEADLVFVIGGK
                     NSSNTTKLYEICKKECPKSYHIENVKELDESLLEDESVKTIGITAGASTPDWIINEVI
                     SKIKEL"
     gene            15789..16583
                     /locus_tag="K8M77_001159"
     CDS             15789..16583
                     /locus_tag="K8M77_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085667.1"
                     /translation="MNIVRFNLWDYFKYARILSLSLKRLCTNCFVKVTYKNEVYMLWE
                     IDYVANFDAYIKKITKIGSMRNIPEKSGVFSEFNISKYIYETVKEDEGIDLKIYGFEK
                     RASNIMLKIDISNFNKKNSENFTFTPFVKGRDESIRCKVQNEIFYEENRIPLKTKDIV
                     YECNRKAFLEDLAFFELKDDIIIGYGQILLLKDKYTIANFGIIEEFRGKGYGEALLIH
                     ILNQAKIKGLKEVYIKVKSDNINAVNLYKKTGFKEASEISIYELLG"
     gene            complement(16681..16830)
                     /locus_tag="K8M77_001160"
     CDS             complement(16681..16830)
                     /locus_tag="K8M77_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085668.1"
                     /translation="MYDLKELEKNQSNQEGANLEISDRSYYSNHLTDSVFKTKISYVP
                     TDSLL"
BASE COUNT         6554 a         1782 c         3161 g         5412 t
ORIGIN      
        1 atttgatatt caaaatattt gaattttata tctaataatt ttgaagtaag ggagcgattt
       61 tagaatgaag gaaagtgaat tagtattaaa taatatttta gttaaattat tcaataatat
      121 tttgaggata gaggagtttg cattaaaaag tgctcctttt aatgatttat ctataacaga
      181 aatgcatact atagaggcta tagggataga aagagccaga acaatgtcag aggtggcatt
      241 agatttaaaa ataacagtag gtactttaac aactgctata aacaaattaa taaaaaaagg
      301 atatgtaaat agaagaagaa tagaagaaga tagaagagtt gtaatgatag aacttacaga
      361 gaaaggaact ttagcttata aagtacatga aaaatttcat gaagaaatga tagaacatgt
      421 attagaggaa ctaggtatta atgaagaaga ggttttaata agttctttag gtaagttaga
      481 taagtttttt caaaagaaat tagaacttat gaaatcaaag gagaattaat atggaaaatg
      541 ctaaaatcat aggctttggc ttatatacac ctaaaaattt agtagaaaat gaaagattac
      601 aagaattttt agagactagt gatgaatgga ttagaagtag aactggaata gaaagaagat
      661 atatttcgtt agatgaaaat acttctgatt tagcagtaga ggctagcaaa aaagctttaa
      721 atcaagctgg gttaagtgct gaagaaatag atttaataat agtcgctact gtaactccag
      781 ataattttac tccatccaca gcttgtattg ttcaagataa gttaggagca aaaaatgcat
      841 gggcctttga tataaatgca gcttgtactg gatttatata tgcacttaaa ttaggacgat
      901 ctttaataag atcaggtgaa gctaaaaatg ctttaataat aggtgcagaa actttatcga
      961 aggcacttaa ctgggaagat agaggaagtt gtgttctttt tggagatggg gcaggggcta
     1021 tcgttcttac aagcacagaa gaagattgtg gaataaaatg tgtaaatgta aaatctgatg
     1081 gttctaaagg ggattccctt gttattcagg ggttacctct aaatagtcca tttaaagatg
     1141 gaagagaagt ttctaaaaat tatataaaca tgaatggaag agaaatattt aagtttgcaa
     1201 caaaagtaat ggaagaaagt atagtggaga ttttagaaaa agaaaacata aaaatagaag
     1261 atatatccgc tataattcct catcaagcaa atttaagaat tatagattac gtagttaaaa
     1321 gattaggaat accaagagaa aagtttgtaa caaatttaca gaattatgga aatacatcag
     1381 gagcaagtat tccaatagcc ctttgtgaat caattaatga aggtaattta aaaaaaggag
     1441 acaacataat aatggttggc tttggtggag gccttacttg gggagccgct ttaattaagt
     1501 tataattctt gaaaaaacaa aaatctagaa aataatacta aatgagaata taattataga
     1561 tatatttatt tttaatccgt taaattttgg gaggtatttt aatggctaaa ctaggatttt
     1621 tatttgcagg gcaaggagca caatatgtag gaatgggaaa agaattcttt gataactttg
     1681 aagaaagtaa agaagtattt aaaagaagta gtgaagcttt aggcatggat atggaagaac
     1741 tttgttttag tgatcctgag ggattattaa ataaaactga atttactcag cctgcaataa
     1801 taactacaaa catggctata cttaaagcac tagataaatt aggagttaaa tctcatataa
     1861 gttgtggatt aagcttagga gaatattctg cacttattca tagttgtgcc attaattttg
     1921 aagatggagt aaaactagtt aaaaaaagag gtaaattcat gcaagaagct gtagcagaag
     1981 gtattggtgg aatggttgca gttttaagaa tgactccaga acaagttgat gaaataatag
     2041 aaaaaagttc ttcatatgga atagttgaag gtgcaaatta taattcacct ggtcaaattg
     2101 ttatttctgg agaattagta gctttagaaa aagctatgga atttataaaa gaagttggtg
     2161 gaagagctat aaaattacct gtatcagctc catttcactg ttcaatgtta caaccagcag
     2221 ctgaaaagtt agaagatgag ttaaataaaa tttcaataaa caaaataaat ggaattgtta
     2281 tgtctaatgt taagggagaa gcctatttag aagaggataa tataatagaa cttttaactt
     2341 ctcaagttaa aaagcctgtt ttatttataa atgatataga aaaaatgatt gaaagtggtg
     2401 tagatacttt tattgaaata ggtcctggta aagctttaag tggatttgtt aaaaaaataa
     2461 ataaaaatgt tacagtgctt aatgtagagg atttaaaatc tttagaaaaa acattatcaa
     2521 aattaagaga gatggaggta ctttaaccta tgttaaaaga taaagtagca atagtaactg
     2581 gtggaacaag agggattgga agagcaattg ctttaaaatt agcagataat ggagctaata
     2641 ttgttataaa ttatagaaat tctgataaag aggcagaaga attaaaagca attttagaag
     2701 aaaaaggggt aaaagttctt actgtaaaat gtgatataag taattttgaa gattctaaaa
     2761 atcttatgga taagtgcaag gaagtatttg ggaaaataga tatacttgta aataatgcag
     2821 gtataacaaa ggatacttta attatgagaa tgaaggaaga agactttgat agtgtaatag
     2881 atgtaaactt aaaaggtaca tttaattgtg caaagcatgc ttctgccata atgttgaaac
     2941 aaaggtttgg taaaattata aacatgactt ctgttgtagg tatagctggg aatgtgggtc
     3001 aagtaaatta tgcagcatca aaggctggtg ttataggatt aactaaatct ttagctaaag
     3061 aattaggaag tagaggaata actgtaaatg ctgtagcacc tggatttata aatactgata
     3121 tgacagcttc tttatctgaa aaagttaaag aagaagcttc taaaaatatt cctttaaaaa
     3181 gattaggaga ccctgaagat gttgctaact tagtaggatt tttagcatca gatgcagcaa
     3241 attacataac aggtcaagtc ataaatgtag atggcggaat ggtaatgtag gaggagtaat
     3301 tattatggaa aacagagttg ttataactgg attgggagca attacaccaa ttgggaacaa
     3361 tgtaaatgaa ttttggacta atgctaaaaa aggtgtttgt ggaattgatg aaataacttt
     3421 atttgatact agtgattata aagttaaaat tgcagcagaa gttaaaaact ttaaagcaga
     3481 ggagtttata gataaaaagg aagctaagcg tttagataga tttactcatt ttgctcttgt
     3541 agctacagaa gaggcaatga aggatgctaa attagactta aaggatgaaa atgtagatcc
     3601 aaatagagta ggagttatag ttggttcagg aataggtgga tatggcacct tagaatcaca
     3661 atttgatgtt ttattaacta aaggaccaaa tagagtttca ccttttgcaa ttccatcagg
     3721 aataataaac ttagttgctg gaaatattgc tattaagtat ggggcaaaag gggtatgtga
     3781 atcactagtt actgcatgtg ctacaggggt aagctctata ggtgcagctt atagaagtat
     3841 aaaacatgga tattcagata tagttatatc aggtggatca gaggcttcta taactagagc
     3901 tgccattgca ggatttacca atcttaaagc attaaatatt aataatgatc ctaaaagagc
     3961 ttcaatacct ttcgataaag aaagatctgg attcgttatg ggagaaggcg ctggaatatt
     4021 aatattagaa agtttagaaa gcgctaaaaa aagaggtgct agaatttatg gagaaattat
     4081 aggatatggt gcaacttgtg atgcatatca tataacttca cctgatccag aaggtgatgg
     4141 agctaagaga gctatagaag cagctataga agaaggaaaa ataaataagg atgaaatttc
     4201 atatataaat gcacatggaa caagtactcc tttaaacgat aaatttgaaa ctttagctat
     4261 taaaggatgt tttggtgaaa aagcttatga cataccagtt tcatcaacaa aatcaatgac
     4321 tggtcattta ttaggtgcag ctggagctat agaatcagta atatgtgtta aagctttaga
     4381 agaaggattt attccaccaa caataggata taaagttcct gatgaagatt gtgatttaga
     4441 ttatgttgta aatgaaggta gagaagcaga attaaattat gtaatcaata atgcattagg
     4501 ttttggtggt cataacgcat cattattatt taaaaagtgg gattaggagt tgaaattatg
     4561 aatcttaatg agattaaaga gcttatagat aaaatcgatt cttctaatat tgcttatttt
     4621 gaggttaaag tagataattc atatataaag atggataaat ctctaactag aaatttagtg
     4681 gaagaaaaga aagcttcaaa tatagaagaa acagaaaaaa ctgatatatc agaagaaaaa
     4741 aatgaatcaa tttcaaaggt tgaggaagta aaattacaac ctaaagaaga gagagttcag
     4801 gatgaagact tatatttagt tacttcacct atggtaggga cattctatga atcacctgga
     4861 gttggagaaa agagttatgt ttctatagga agcaaaattt cttcaggaga tgttttatgt
     4921 atagttgaag ccatgaaact tatgaatgaa attgaaagtg aagtatctgg agaaattatt
     4981 gatatattag ttcaaaatgg aaatatggtt gaatatggtc aacccttatt taaggttagg
     5041 agagcataat tatgatgaat ataaatgaga ttaaagaaat acttcctcat agatatcctt
     5101 ttttacttgt tgataaagta gaggagataa ctgaaagtaa agttgtagcg tataagaatg
     5161 taacgataaa cgaacctttt tttcaaggac attttccaga ttatccagta atgccagggg
     5221 ttttaatagt agaggcttta gctcaggcag gtgctatagc actattaaat aaagaagagt
     5281 ttaaaggaaa aactcctttc tttgcaggaa tagacaaagt cagatttaaa aaacaagttt
     5341 taccaggtga tactctaaga cttgaagttg aaataataaa acttagagga agcattggat
     5401 ttggtaaagc tacagctact gtagatggaa aaatcgcttg ttctggagaa attatgtttg
     5461 caataggtta ggtgatttaa ttatgtttaa aaagatatta atagcaaata gaggtgaaat
     5521 agctgttaga ataatacgtg cttgtagaga attaggaatt cttactgtag ctgtttattc
     5581 tgaaatagac aaagatgcat tacatgtgca gttagctgat gaagccgttt gtataggtcc
     5641 atggaattct agggaaagtt acttaaatat tcaaaatata ttaagtgctt gtgttttaac
     5701 tggagctgaa gctattcatc ctggatttgg ttttttatct gaaaatccaa gatttgctaa
     5761 gatgtgtgaa gagtgtaata tcgagtttat tggccctaat tggagagcta tagagcttat
     5821 gggaaacaaa gctatggcaa gagaaataat gaaaaaggct gatgttcctg ttgtacctgg
     5881 atatgaaggt gaaattgaaa gtccagagtt tgctttaaag atagctaaaa aaataggctt
     5941 ccctgtaata gtaaaggctt cctcaggtgg tggtggaaaa ggaataagaa tagtttataa
     6001 agaagaagat tttatttcaa attacaacac agctaaatca gaggctcaag caaactttgg
     6061 tgatgataaa atttacattg agagatttgt tgaaaatcct aaacatatag aagtacaatt
     6121 attatgcgat aagcatggaa atgctcttca cctttttgag cgagattgtt cagctcaaag
     6181 aaaaaatcaa aaagttttag aagaagctcc ttcatcaatt ttaagtggtg aacaaaggga
     6241 taaacttggt aagattgcag ttaaagcagc taaagctgta aactatagca atgttggaac
     6301 aattgaattt ttattagata aaaatggtga attttatttt atggaaatga acacaagaat
     6361 acaagttgaa catcctataa ctgaaatgat aacaggtgtt gatcttgtta aggaacaaat
     6421 aaaggttgca tttggtaagg agttagaaat aactcaagat gatttaaaga taaatggtca
     6481 ttccatagaa tgcagaataa atgctgaaga tccaagcaaa aactttatgc catgtcctgg
     6541 cctaataaag gaaattaatt tcccaggagg atttggagtt cgtttagata cagcaattta
     6601 ttctggatat aagatacccc attgttatga ttcaatgatt ggtaagctaa tagttcatgg
     6661 taaagataga gaagaggcaa tttctaaaat gaaaagggct ttagatgaat ttgctattga
     6721 tggtataaca actaacatag actatcactt tttaatattg gatcatgaag attttaaaaa
     6781 tggtacttat aatacttcat ttttatctga aaagttggtg aataagtatg attaagaatt
     6841 tattaaataa aagaaagtat ataactgtta gttcagttga attaaatgat actgaattaa
     6901 gtgaagatga aaaaccaaat ataccctctg gtatgtggag caaatgtgaa aagtgtgcaa
     6961 aaatattata tactgaagat ttaagagaaa attttaatgt gtgtcctaat tgtggtcatc
     7021 attttaagtt aggagcatat gaaagaatta aatatttaac agatgaaaat acttttgttg
     7081 agtttgacaa aaaaataatt ggaagaaatc ccttagattt taatggatat gaagagaaga
     7141 ttaaaggata tcaaaagaaa agtcatgtta ttgaaggggt tgtaacagga gaggcttata
     7201 ttgctcagag aaaagtggtt ctttgtgtaa tggattctaa ttttatgatg ggaagcatgg
     7261 gaacggctgt tggtgaaaaa attactagag ccattgaata tgcaactaaa aatagacttc
     7321 ctttaataat atttacttgc tctggtggag caagaatgca agagggaata tattctctta
     7381 tgcaaatggc aaaggttagt ggggcaattt ataaacatgg tagagagaac ttattatata
     7441 ttacagtatt aacaaatcct actactggtg gggtaactgc tagctttgct atggaaggcg
     7501 atatcatatt aagtgagcca ggatgtttag ttggttttgc agggagaagg gtaatagaag
     7561 gaactattaa tgaaaagttg cctgatgatt tccaaactgc agaattttta ttggaaaaag
     7621 gatttataga taagatagta caaagaaaag atttaaaaca agtaataact tctcttttaa
     7681 gaatgcatga ggtggattat gagtagagaa ctaataagaa cagcagatgc atggaataaa
     7741 gtaaaaatag ctagagatcc taatcgtcct aatgcaaaat tttacataaa cgaaatattt
     7801 gatgaattta tagaattaca tggagataga aattttggag atgataaagc aataataggg
     7861 ggaatagctc ttttaaataa tctaagtttt actgttgtag gtatatgcaa gggtgaaaat
     7921 acaaaggaaa atataaaaag aaactttggt atgcctcatc cagaaggata tagaaaagca
     7981 ttgagactta tgaaacaagc agaaaaattt aaaaggcctg ttatttgctt tgtagatact
     8041 ccaggagctt tttgtggtat aggtgcagaa gagagaggac aagggcaagc tatagctcaa
     8101 aacttagttg aacttatggg attgaaggtt cctttaatat caatagttat tggagaaggt
     8161 ggaagtggag gtgctttagc cttagctgtt gcagataaag tatttatgtt agaacactct
     8221 atttattctg tattatctcc agagggcttt gcatctatat tatggaaaga ttcttctaga
     8281 gctgaggaag ctgctagtgt aatgaaaata actgctcagg atttaaaaag ctttaatata
     8341 atagataaaa taataaaaga acctagggga ggagcacata aaaatccaat taaaatggct
     8401 caaaacatta aaaagacaat tttagaagcc ttaggtgaaa tgaagggtac tgatttagat
     8461 accttattaa atgaaagata caataaatat agaaatatag aaaataatct ataatcatat
     8521 aaaataaaac acatgagttt tttagctcat gtgtttttta attaaaatta attcctcttt
     8581 aaattaatat ttacttaaag agaggattaa aatttagttt gtaagcattt ttattagtat
     8641 atctattatt gacttgtttt tccttaaggc atctacataa tcagcaacgt gaagtttata
     8701 atactctttg ccttcatctg ttattctata catctttttt gttcttttat ttataaagtc
     8761 atcccctatc caataacttg taactaagcc tttttgctct aagtcataaa gtgtatcata
     8821 aattgtgcta gatgatactg ggaatggaag aggagcacta gaaaatttat ctctaaaagc
     8881 ttctaaaact ttattaccaa aaacctcatg tccctttgaa agttctttta gtacataaaa
     8941 agaatataat tgttttgcat ttataatatt tcttggtgcg accatgcgag ttcttctatc
     9001 caaaaaaact tcacctccaa ttttacattt tatattataa gtataactca atttgttaca
     9061 aaaagtaatt aacaatttgt gatttcttga atatactata ctatacaaaa gaaacgaggt
     9121 gaaatattat gtcaagaaaa tgttgttgct catgcaattc ttgtagaaga aattcctgtg
     9181 gatgcagatg tggaaataat tgctgtggat tcaataattg tagcggtttt ggaaactgtg
     9241 gtggattcaa cagttgcgga ggatttggta attgtggagg atttccttta ctttggcttc
     9301 ttctattagg ctgtggcggt tttggaaact gtggcggaaa ctggttttaa ttaatatcta
     9361 attttaaata tagactagtt atataagata ggaggttttc tcctatctta taatttttca
     9421 taagtttctt ttagtaataa ggcatctttt tctaagagag gactttcaca tattatacat
     9481 ccctttactt tataatcctt aagtgccctt aagcattcaa taaagttaaa atcactttct
     9541 aaaaaaggta agtggtttct ttcccctttg tctgtgtagt gaattccaga catatgaata
     9601 tgtaaatctt ctaaggcttc atttcctaaa ttatctttta tatattctaa aatttctgcg
     9661 aaatctttat attcttttaa agcaccaatt cctctagcat gtatatgaga aaaatcaata
     9721 cataatttgc aagatggcac ttctttacat atatcaacta attcttgtag gtttccgaat
     9781 tgagttcctt taccagtagt ttctaatcta taatctacac cttcataagg taatttcttt
     9841 aagtttttct ttatttcctt taaagcttct tcttttgagg cttttaaata aaatcctgga
     9901 tggaatacta agcttctacc tccaacactt tttaatgctt cagcaccttt aagtattctt
     9961 tcaatagatt tttctttttt ttcctcttct tcagcattta aatttataaa ataagaacca
    10021 tgagctgaaa gataaaaatc atttttttct ttagcttcta ttattttttc tctgttttta
    10081 gctgtaacat taacggatct aacaaaagga agctccatag cttctagtcc tatttttttt
    10141 aagtattcaa ttcctgttga gtaattgaat ttctggccct cttcacctat tggtaaacct
    10201 gaaattccaa ataaaagttt ttccatgtta tcacctctta ctaaagtata gcaaaacact
    10261 aggaattctt caatagaaaa gaattattgt ttaatatgaa ttataatcta tcaaatttat
    10321 taatttatac ataatttttt aagttttttt cagaaaaaat gcaattattc gaaataaata
    10381 ttgcatttta cactatagtt atttaaaatt attaatagaa actatcatat ggagtgtgaa
    10441 aaatggaaga aagattgcaa aaatacatgg caaggtgtgg agtagcatca agaagaaaat
    10501 gtgaagaaat aatttgcagt ggaaaagtaa aggttaatga cactgttgtt gactcccttg
    10561 ggactaaggt tgatccatca gtagatgttg ttagcgttaa taataaagta ataagtcctg
    10621 aggagaataa agtatatata atgctaaata agcctgaagg atatgttaca accaataaag
    10681 atgaaaaaaa tagaaagacc atattagata tagtaaaagt gaatgaaaga atatatccta
    10741 taggacgtct tgattatgac agttcaggtt tacttttatt aactaatgat ggagatgtat
    10801 acaataagat aatacatcca agagttaatt taaataaaga atatattgcc ttagtaaaag
    10861 gaatttttac agaggaagaa atgaataaat ttagaaaagg cgtagatata ggagattata
    10921 aaacagcaga atgtgaaata gaattactag aaaaatacac aaatagctgt aaagttaaaa
    10981 taatcattca tgaaggcaaa aatagacaga taagaagaat gtgtgctgcc tttaatcatg
    11041 aagttgtttc tttaaagaga gaagctattg gaagcttgac cttaaagaat ttaaagaaag
    11101 gagaatggag atatttaagt agttcagaag agaattatat aaaatctcta tagtaattat
    11161 gtttaaatat gtatctgatg taagtaatat atatcataat ataataactt ctttcataga
    11221 aaataaaaat atagcaatag acgctacctt aggaaatggt catgattgtg attttttaag
    11281 tgaactattt aaaaaggtat atgcctttga tatacaagaa gaggcagtaa gttcatataa
    11341 atcaaagaat aaagaaaatg ttgaagttat tttagattca catgaaaact ttgaaactta
    11401 cataaaagaa gaaaatgttg attgtatagt ttataactta ggatatcttc caggtaataa
    11461 taaggaagta actacggttg cacatagtac tttaaaaagt atagaaatag gattagattt
    11521 attaggtgat aatgggttaa tgataatagc cctttatagt ggacatgagg aaggtaaaaa
    11581 agagaaagat gccgtacttc agtttacaag taaattacct aaaaataaat atggtgtttt
    11641 acatacagaa tttacaaaca gagctaagag tgcaccttct ttagttgtta ttgaaaagaa
    11701 gtttaaataa gatttaagta agtatagaga ggatttttta aaatggaaag tcaagattta
    11761 atgagattaa ttcaagttaa gtttccaaag cttagtaaag gacaaaagct tatagctgaa
    11821 tatatattaa agaattatga taaggctgca tttatgactg ctgctaaatt aggaaataat
    11881 gttggagtaa gtgaatctac agtggttaga tttgctacag aacttggttt ctcaggatat
    11941 ccaaagttac aaaaagcact tcaagaatta ataaagaata agttaacatc tgttcaaaga
    12001 ttagagttat ctaatgattt tataagtgaa gaaaatgctc taaaaggtgt tttaaaagca
    12061 gatatagaaa atataagagc aacattagaa aagataaatc ataaaacttt tgatgaagtg
    12121 gtaaatgaaa tattcaaagc taaaagaata tatataatag gacttagaag ttctactgct
    12181 cttgcagaat ttttaggatt ttacttaaat ttaatattag ataatgtaag agtaatttca
    12241 aatggaataa gcgatacttt tgaacaaatg cttaacttag gagaaggtga cttagttata
    12301 ggtataggtt tcccaagata tgcattaaaa acaatagatg ctttagaatt tgctaaaact
    12361 agaaatgcta agatagtttc tattacagat agtttaattt ctcctttagc atctcaatct
    12421 gatcatacac ttatagctca aagtaatatg gcttcatttg tagattcatt agttgcacct
    12481 ttaagtgtaa taaatgcatt aatcatagct gttggtataa gagaaaaaga gaagataaca
    12541 tcaacattta gtgagttaga aaagatatgg aaagactata atgtttattc atataataaa
    12601 aataatatag aaccttaata aaaagctgta taaaggtata aagaacatat aaattatttt
    12661 aagatagagc ataaatttta ttctattata taatttataa gtttcataaa ttatatcttt
    12721 atatagcttc atttttcgaa attacataga atattaaaga aatgcatttg ttgacaaatg
    12781 tatttaataa agtttattat tagtttgatt caattaaatt aaggagctga taaattggct
    12841 aaagtaatag ttataggcgc cggtccagca gggatgatgg cagcaatatc tgcagcagaa
    12901 aatcatgagg ttattctttt agaaggtaat gaaagaatag gaaaaaagct atttattact
    12961 ggtaaaggta gatgtaatgt aacaaatgca aaggatataa gtgaattttt tgattttata
    13021 ccaggaaatc cacatttctt gtatagtgca ttatatactt atactaatat tgatgtaatg
    13081 aacttttttg aaaatgctgg agttaaatta aaggtagaaa gaggatcaag agtttttcca
    13141 aattcagata aatcttcaga tataataagt ggattatcaa ggggattaaa tgaagctttg
    13201 gtagacttaa gattacatag taaagttaaa gatgttattt ttaataacaa taaaatagaa
    13261 gctgttattt tagaaaatgg ttcaaaggtt aagggtgact attttataat aacaactgga
    13321 ggtaagtcat atcctcttac tggatcaact ggaataggat ttgatttagc taaaaagatg
    13381 ggtcacacta tagtagaacc aaaaccatcc ttagtaccta tagaaataga agagtcatgg
    13441 gttcgtgaat tacagggcct atctcttaga aatatagagc ttaaaataaa gaataaaaag
    13501 aattctaaag tagtttatag tgggcaggga gagatgctat ttactcattt tggaatatca
    13561 ggacctcttg ttttaagtgg gagtagattc ataaaagatg gtgagaaatt tgaaatttct
    13621 ctagatttaa agcctgcctt agaggaaaaa cagttagatt taaggattca aaaggatttt
    13681 aaaaagaatt taaataaaga ttttaaaaat tctttagatg aacttcttcc aaagaaactt
    13741 atacctgtta ttatagagtt atctaaaata gatgaaaata aaaaggtcaa ctctataaca
    13801 aaggaagaga gaagaacatt attaaatcta ttaaagaatt taacttttac agtaaaagga
    13861 ttaagagata tatcagaagc tattgttact gcagggggag tttcaactaa agaaatagac
    13921 ccttcaacaa tgcaatcaaa gattgtagat aatctttact ttgcagggga agttatagat
    13981 gtagatgcct ttacaggggg atataatgtt caaatagcct tatcaacagg atatttagca
    14041 ggaaaatcaa tttaatttta aaattatagt tgctataaag ttaataaaaa ttttagttag
    14101 tttagtagaa actttatagt aaacttaaag agagtataaa ttttacaatg tttttatgag
    14161 aaaggactta agaattatga ataaactaat tacagttgct attgatggac ccgcaggagc
    14221 tggaaaaagt actattgcta aaataatagg agaaaaattt aatttaatgt atataaacac
    14281 aggatctatg tatagagctg ttactttaaa agccttagaa aataatattt cagctgaaga
    14341 agttgataaa ttattagtta tgatagatgg aatggatatg cattttgaaa atgatgagct
    14401 tatattaaat ggagaaaata taaactcatt aataactatg cctaacataa gtaaaaatgt
    14461 ttcagcttat gcttcaataa gagaagtaag ggaaagatta gttaatctta tgagaaaaat
    14521 ggccttaaaa tatagcgtaa taatggatgg aagagacatt ggaacagttg tattaaaaga
    14581 tgctaatttc aaatttttct taactgctag cccagaagaa agagctgata gaagatacaa
    14641 agagcttatg ggaaaaggtg tagaagttaa ttatgatgaa atattacaag atataataaa
    14701 aagagattac ctagattcta atagagaagt agatcctcta agaaaagctg aggatgctat
    14761 tgaaattgat actaccggca taggcataat gggggtagtt gaaaaaatat ctagctatat
    14821 ggagaaataa tatggaaaga aacgtaattt tagctaagaa tgctggcttt tgctttggag
    14881 taaaaagagc agttgatgaa gctataaaat atcaaaaaga atttggaaaa aaaatatata
    14941 ctttaggacc acttattcat aacaatgatg ttgttaatta tttagaggat aatgatatat
    15001 ttgcaattga actttcagat acgaattctt taaataaggg ggatgttgta cttataagat
    15061 cccacggggt taaagaaagt gtaataaaag atttaacaga caagggttta attgttaaaa
    15121 atgcaacttg tccatatgta actaatattc agctaaaggt taaaaaatgt tatgaacaag
    15181 gatataagat aataattgtt ggggatgaaa atcacccaga agttattgga attaatggct
    15241 ggtgtaatga ttctgccata ataacaaatg gaaaaacaga acttgaaaat attccagcaa
    15301 aggtttgtgt agtttcacaa actactgaga aaaaagaaac ttggaacaag gttttaaatg
    15361 aaatagtaaa agaaagtaaa gaaatagttg cttttaacac tatttgttca gctactgatg
    15421 ttagacaaaa aagtgtacaa gagctttcaa aagaggctga tttagttttt gttataggtg
    15481 gaaagaatag ttctaataca acaaaactat atgaaatatg caaaaaggaa tgtcctaaga
    15541 gctatcatat agaaaatgtt aaagaattag atgaaagtct attagaagat gaaagtgtta
    15601 aaactatagg aatcactgct ggggcttcga ctccagactg gataataaat gaagttattt
    15661 ctaagattaa agaattataa tttttaaata taatcctgca atttaattca taattatttt
    15721 gtactactgg tattaaactg ggaattgttt tatactataa tatatgacga agttttagga
    15781 gtagtagtat gaatatagtt agatttaatt tgtgggatta ttttaaatat gcaagaattt
    15841 taagtttatc cttaaaaagg ctatgcacta attgttttgt caaagtaact tataaaaatg
    15901 aggtttatat gctttgggaa atagattatg tagcaaactt tgatgcatat ataaaaaaaa
    15961 ttactaagat aggtagcatg agaaatatcc ctgaaaaaag tggtgttttt tcagagttta
    16021 atataagtaa atatatatat gaaactgtaa aagaagatga gggaattgat ttaaaaattt
    16081 atgggtttga aaagagggct tctaatataa tgttaaagat agatatttct aattttaata
    16141 aaaagaatag tgaaaatttc actttcaccc cttttgtaaa aggaagagat gaaagtattc
    16201 gttgtaaggt acaaaatgag atcttttatg aagaaaacag aattccacta aaaacaaaag
    16261 atattgttta tgaatgtaat agaaaagctt ttttagaaga tttagcattt tttgagttaa
    16321 aggatgacat tataattgga tatggtcaaa tattgcttct aaaggataaa tacactattg
    16381 ctaactttgg aataatagag gagtttagag gtaaaggata tggtgaagct ttacttatac
    16441 acatattaaa tcaagctaag ataaaaggat taaaggaagt atatataaag gttaagtctg
    16501 ataacataaa tgctgtaaat ttatataaaa agactggctt taaagaagct tctgaaataa
    16561 gtatatatga attgttagga taaattatat gcatgaaaat aaatctttaa taaaacttac
    16621 aaatccctaa agaaaaatta gaactttttc ttgaggtatt tgtaagtttt attattgagt
    16681 ttataaaaga gaatctgttg gtacatagga tatcttagtt ttgaatacac tatctgttaa
    16741 atggttacta taataagaac gatcgcttat ttctaagtta gctccttctt gatttgattg
    16801 atttttttct aattctttta aatcatacat aatattcacc tcctctttaa tagtattgac
    16861 aactttagaa aatcttattt agagttagaa aatattttaa tatggacaa
//
LOCUS       DAEVGI010000040        16902 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEVGI010000040 DAEVGI010000000
VERSION     DAEVGI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 16902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16902
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.040"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..1350)
                     /locus_tag="K8M77_001161"
     CDS             complement(<1..1350)
                     /locus_tag="K8M77_001161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085669.1"
                     /translation="MSKINKLRFVNLNYNHNTMKIDDETFYLDGQDTMLNLRNGGGKS
                     VLVQMVMAPLVNKRYRALKDRTFESYFTTNTPTYIMIEWILDDGAGYLLTGMMVRKKD
                     VLSDEDSKDKLDIINFIHEYKEKNEYDIKRIPIVEINNGKKMVKSFLNSKKLFEELKK
                     NKEVRFNYYDMNNPVTTKNYFEKLQEYKINHKEWETIIKQINLKESGLSELFIKAKDS
                     SGLVKSWFLPAVENKLRKDEDRIKNYRNLVKRYIEQYKANKANIDKKEKIELFNKLSE
                     DIKNSCDEFILTIEERKGLENKIANIIRILKNNYEDKENEEKELECLINQLVEKIAEL
                     FYEEYSIKIYRKEDKIEELEEYVKELDNLVNKSKEEKKALNRKNEILECSKIHKEYQS
                     RSQELQSLESELKILTKKKDDNTPHINNIGFTIREILNKEISDMKEKVDERKTDINVL
                     "
     gene            complement(1343..2050)
                     /locus_tag="K8M77_001162"
     CDS             complement(1343..2050)
                     /locus_tag="K8M77_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085670.1"
                     /translation="MAYSMEEIKISNKIFYYLLKHGELKEEENDLYKSYSENNNVTEL
                     VKELGESSECYIKKYGGVVYLIPREENDFLGYSKGELKKILCRSNANDKDYYLSQFVI
                     LTLLVTFYNSKGVSSKGRDYMKIGEFLNIITDRLNEGAERTKEEDSSGIAFSNILERF
                     EALKSSDKRSTSKTTKGGFINNILDFLEKQGLIDYIKNDDMIKTTKKLDNFMDWNILN
                     KNNYNRVLKALGESVDE"
     gene            complement(2016..3665)
                     /locus_tag="K8M77_001163"
     CDS             complement(2016..3665)
                     /locus_tag="K8M77_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085671.1"
                     /translation="MDFDFLDVQHIRMKRIGAYALLFRNSIAKGTWKKYGFSEGYEQD
                     NLIFAVLLYIMEEALKDDMCTIDNIGYFIDEINSIHFKKELSYEECKELAEFIVNTIL
                     CDEGKAMYFKAYNFKKVQYEDINISFIKNSMKYIDDVRRVVYSLTDDGYSFILSTLEV
                     EENLKITIHEILLKMHLEKASYDKAVDDIKNIFNQYRIRVKSIEDAIRKIKENPLSYT
                     TEDYEKMMSGNLNLLNEIKKQLIIHRGMVELRINEFLERDIHIRKLNKKEEENLNNLK
                     IIQNYLGRTIDEDQKLLKKHFDLKDVYGMELETISKMALIERFNFRNEVYDKILDNPN
                     KLEDIDIFLRPLLKRELPKKYNINKSLEYQKNINKNYSEEDEELSFEESDFIDEINKK
                     KIEKLKKYNNVIKLILELSMKQGGISLREIQGLIKESNELMKKLIPTVEIFREVIIEM
                     LKHRNININEIREERKNAIENGEIEFQLNKSILEVLDNNSELDSIKEINIKKLIDKDD
                     IKLEGVKTEDGEFKNFICSDILFEAIKEDKNGLFYGRNKNK"
     gene            complement(3674..4675)
                     /locus_tag="K8M77_001164"
     CDS             complement(3674..4675)
                     /locus_tag="K8M77_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085672.1"
                     /translation="MSLSSFKRKRIELDELEKLFKIDTYCDLYVKVNELISENKIKPI
                     ISSGQNGKSPTLYKRYTIIDIKEDNKELIDEIEYKLISKFNIEYYRNHLEKYIDHRKY
                     ILQLNDFFINKSEFLNYPVSMNERAFQIWGREKFLQKEEGKSILKNLGIDLGMLNYYD
                     TSEPLAYYSRSKNTPQNVLIIENKDTYYTLRNYLISNNKILGKEITTIIYGAGKNVWK
                     AFKDFRVSVENHVSNKNNKFFYFGDLDYEGILIYEKFYHMFSEEYNIRPFIKGYEKMV
                     EKSEMMEINLPKTKAGQNRNIENVFLQEFSEKLNGKILKILENNLYIPQEIINLTDL"
     gene            complement(4915..5922)
                     /locus_tag="K8M77_001165"
     CDS             complement(4915..5922)
                     /locus_tag="K8M77_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HBI7085673.1"
                     /translation="MSEKITFCEYCRDDVSYIEKEEYMISKLKGETYKYYGKKAICKE
                     CNNEVFVSEINDYNLNQLYNEFRKQNNIISLEHILEIPNKYKIGKRPLSLLLGWGEQT
                     FSRYCDGDMPTKQYSAILEKIYKEPSYYLELLEENKDRLKPIAYDKSKEATEILISEQ
                     QNLENSKIESVVNYLLCECEDITPLALQKILYYIQGFYYAFLKKFIFEENCEAWVHGP
                     VFRNIYYKYKEYRFDPIKEESINKQFDLSISEKVIIDSVIKNLGCYSGKVLEAFTHAE
                     MPWIETRGDLSASIGTDRIIDKRLIADYFNSVKEKNNMINPSDIGDYSKKMFEQILDN
                     N"
     gene            complement(5953..6368)
                     /locus_tag="K8M77_001166"
                     /pseudo
     CDS             complement(5953..6368)
                     /locus_tag="K8M77_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409522.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6879..7106)
                     /locus_tag="K8M77_001167"
     CDS             complement(6879..7106)
                     /locus_tag="K8M77_001167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085674.1"
                     /translation="MNLNDIEVKIKNLIDNKTYKNSEFIYEFLLCFDLPKASITRLKK
                     GDYNIAKDKTDILWKKKIFFKECSNNIYEEY"
     gene            complement(7261..8445)
                     /locus_tag="K8M77_001168"
     CDS             complement(7261..8445)
                     /locus_tag="K8M77_001168"
                     /inference="COORDINATES: protein motif:HMM:NF013601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBI7085675.1"
                     /translation="MVRKKFDWSKFEINHFHFFINQKINQYGGTIAHVDYGEDNYYEE
                     DKYYETIIEQLEVIKEKIKKYNAIFIDEGQDYDKRWFDIIEKYFLANNGEFVIFADEK
                     QNIYNRVELDEDKRIKTNIRGRWNELNKTFRIGIEFSRLAGEFQKEFLKDRYNLDDIK
                     VPDEEEINMFDQIDKNLSDNLNQDSIFNYLNIIDYKDIFKDIEEYVRKKNIEYGEICI
                     LGESIDNLRALEKEVRDSYNIESEITFETEEEYKSIKCSVDEIIQYDTLNCVFKNNNY
                     YSKLLKSRLDEVRRNKKYSFKVVSENLKISTIHSFKGWEIDTLVLILDENTDENVNEE
                     LIYTAITRCKKNLIIYGLRNSKYYKFFSENFKQVNDLQFSMYPVLPEFEFSMSKNNEL
                     IW"
     gene            complement(8539..9252)
                     /locus_tag="K8M77_001169"
     CDS             complement(8539..9252)
                     /locus_tag="K8M77_001169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085676.1"
                     /translation="MDKFTLEETRYNTDVNIVNKLKEDLDNSYEIYYKPNLNGDRPHI
                     LIMKKRIGIYIIKISDWDLSKYSYEKKENDTKKYFYEKNTHKRFRSPFDQVNSCKNNL
                     FEFYSKKLMERKIFNKKYYGVVSCGVYFQHFKESEFKHHIPLSKFMKFNKYINVWGYD
                     SNIVKDIKRWGEDKKYNLFDTEIYDEIFNFINPAIHLEGDGACISFDKKQKSLIESLE
                     NKKVKIKGVAGSGKSLVLA"
     gene            complement(9726..11093)
                     /gene="rlmD"
                     /locus_tag="K8M77_001170"
     CDS             complement(9726..11093)
                     /gene="rlmD"
                     /locus_tag="K8M77_001170"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBI7085677.1"
                     /translation="MVEKNKEYIFDIISQGYEGEGIAKIDNKYPIFIEGALKGEKVKV
                     RIVKVNKNFAYGKLMEVLEASEERVNPPCAIYKRCGGCKLQHASYKAQLDFKWDRVKD
                     CVSKIGKLDPSIVKYPLGMEEPWRYRNKVQLPIGLINGEVKIGFFAPRSHDIIDMESC
                     LIQDEIGDKVVKLTREWIEKFNIRPYNVDGEYDEKGIVRHIMIRRGFTTNEVMVVLVT
                     NGENLPHKEEFVDLIVKNIPGIKSVIQNINSKKTNVILGLESKTLWGEDTISDYIGDF
                     RFNISPLSFFQVNPTQTEVLYGKALEYANLTGNEEVFDAYCGTGTITLFLSQKAKKVY
                     GVEIIPQAIDNAWINAKENKVENVEFFVGESEVVIPDLINKGVKADVVVVDPPRKGCD
                     KKLLDAITNIDAKKIVYVSCDPSTLGRDLKVLEENGYKTLEVQPVDMFPNTAHIENVA
                     LLIKK"
     gene            complement(11395..12954)
                     /locus_tag="K8M77_001171"
     CDS             complement(11395..12954)
                     /locus_tag="K8M77_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBI7085678.1"
                     /translation="MFKNLTFNENEKKYIQIARYIRELINRGVLIEGSKLPSSREISS
                     ILSIGRNTVMSAYEILESEGFVETIKGKGTFVKKEFSNEKSTKVSSIKKENSEIREDK
                     WNIDWKYKTNEYGVLAESLDIIKSEEKWEKGMISFKSIAPPGNLFNIEELKRSFSNRI
                     ALEKHKIVNYGYAVGYKPFIDYLKDYMKEKGAYSEEKEIIVTNGFTEGLDMMLSSFTK
                     DGNYILCENPTHNTALKIMKTHKLNIIGVKMTKDGMDIEDLNKKIEKLGEKIKFAYLI
                     PSYHNPTGVVASIKKREEVYNILKDAGIPIIEDGFNEELLYESSPTIPLASLDRESNG
                     VIYIGSFSKILFPGLRIGWVYGDKKIIGILESVKRCRNIHTSFLDQGILYDFMISGAF
                     EKYIKKVRKVYKEKYKFTKRVIEKYIKDAEIWGEGGLYMFLKIKGVDTRELLKRCYDR
                     GVLFMPGDLFYIDNDLYLEKENRVFSLEEHNIKGREKRDTLRIGFTRLSEEEIEKGIK
                     IIGEEVKSTLT"
     gene            13221..13379
                     /locus_tag="K8M77_001172"
     CDS             13221..13379
                     /locus_tag="K8M77_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBI7085679.1"
                     /translation="MKHNSSIGCTVNECKYHCNEDNYCTLNKIEVVKNTAEACSTECT
                     DCGSFERK"
     gene            complement(13597..15132)
                     /gene="cls"
                     /locus_tag="K8M77_001173"
     CDS             complement(13597..15132)
                     /gene="cls"
                     /locus_tag="K8M77_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI7085680.1"
                     /translation="MEKFLKKLLSRAFIVGFLIFIQFVILMGAIWKVSEDFIYVYFLF
                     IFISIVVFIYIVSRKDNPSYKLAWAVPVLLVPVFGGLFYLIFGGNKTSKKFRKQIKAS
                     YDETAHLLYDDRTVLEELENLDKSIANQSRYIADYSQFPVYKNTTTEYLSPGEVFFER
                     LKEELKKAKHYIFMEYFIVHEGVMWNSILEILEEKVKEGVEVRFVYDDMGCLGTLPYK
                     YNEVLESKGIKCMVFNPFVPLLSLRMNNRDHRKITVIDGHTGFTGGINLADEYINEIV
                     RFGHWKDASIMLKGDAVWNLTVMFLQIWNFYSERKEQYEKYYPYFHHEDEFESDGYVQ
                     PYGDSPLDDEIVGENVYLNIINKAKDYVYINTPYLIIDNELVTALTLAAKSGIDVRIV
                     TPHIEDKWYAHIVTRAYYPQLIESGVKIYEYTPGFIHSKTFVSDDEIGIVGTINMDYR
                     SLYLHFECGVWLYKTKSVEQIKDDFLKTLEKCQRITLEDCAKVKVPTRILRSIIRVFA
                     PLM"
     gene            complement(15303..16100)
                     /locus_tag="K8M77_001174"
     CDS             complement(15303..16100)
                     /locus_tag="K8M77_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI7085681.1"
                     /translation="MNELGKIAVITGATSGIGKEYAFQLAKKGYDLILVGRRVEKIKA
                     VAEEIENNFKVKTHVEILDLTDDNKLDDFIKRLEKKDNIEFLVNNAGYGADDSFTRNE
                     YSKQYDMAKVHMLVTMKLCHSLSKKMKENNKGYIINVSSMAGFNVFPSSTMYCSTKAF
                     LISFTQCLAMELLENNIKVQCLCPGFTRTDFHSKLNMEESKLQNKGFVRWMSTREVVE
                     ISLKNINKKLKVIVIPGFCNKFLYFVSKFTPRWLYYKVAIKGWELMD"
     gene            complement(16228..16584)
                     /locus_tag="K8M77_001175"
     CDS             complement(16228..16584)
                     /locus_tag="K8M77_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBI7085682.1"
                     /translation="MTRLACSAHNCVNYVNGMCSALTIEVDGESAENKQETCCNTFAP
                     RTFVNAIKSAFNTNYVGTIMQALDSQEDVDHKIKCYAMNCTYNVGMTCTSTDIQVFGP
                     EATTSTSTECETFYKK"
BASE COUNT         5505 a         2943 c         1357 g         7097 t
ORIGIN      
        1 taaaacattt atatctgttt ttctttcatc aactttttct ttcatatcag atatttcttt
       61 atttaaaatt tctctaatag taaaccctat attatttata tgaggtgtat tatcatcttt
      121 ctttttagtc aaaatcttaa gttcgctctc taaactttgt aactcctgtg atctactttg
      181 atattcttta tgtatttttg agcattctaa aatttcattt tttctattta aagccttttt
      241 ttcttcttta cttttattta ctaagttatc taattcttta acatactctt ctaactcttc
      301 tattttatct tctttcctat aaattttaat agaatattcc tcataaaata attcagctat
      361 tttttctact aattgattta tcaaacattc taattctttt tcttcatttt ccttatcctc
      421 ataattgttt tttaaaatcc taataatatt agctatttta ttttccaacc cttttctttc
      481 ttctattgtt aatataaact catcacagct atttttaata tcttctgata atttattaaa
      541 aagttcaatt ttttcctttt tatctatatt agctttatta gccttatatt gttctatata
      601 ccttttaact aaattcctat aattcttaat tctatcttca tcttttctta atttattttc
      661 aacagcaggc aaaaaccaag atttaactaa tccagaacta tctttagcct ttataaaaag
      721 ttctgataat ccagattcct ttaaatttat ttgcttaatt atggtttccc attctttatg
      781 atttatctta tattcttgta atttttcaaa ataattttta gtagttaccg gattattcat
      841 atcataataa ttaaatctaa cttctttatt cttttttaat tcctcaaata attttttaga
      901 attcaaaaag cttttcacca ttttcttccc attattaatt tcaactatag gaattctttt
      961 tatatcatat tcattttttt ctttatattc atgtataaaa tttattatgt ctagcttatc
     1021 tttagaatct tcatctgata acacatcttt ttttctaacc atcattccag ttaataaata
     1081 tccagctcca tcatcaagta tccactcgat cattatataa gttggggtat ttgtagtaaa
     1141 atagctttca aatgttctat cttttaatgc tctatacctt ttatttacta atggagccat
     1201 caccatctgt acaagtactg attttccacc tccatttctt aagtttaaca ttgtatcttg
     1261 gccatcaaga taaaaagttt catcatctat tttcattgta ttatgattat aatttaaatt
     1321 aacaaatcta agtttattta ttttactcat caacagattc tcctaacgcc ttcagcaccc
     1381 tattataatt atttttattt aaaatattcc aatccataaa attatctaac tttttagtag
     1441 tcttaatcat atcatcattt tttatataat caattaatcc ttgtttctct aaaaaatcca
     1501 atatattatt tataaatcct ccttttgttg tttttgatgt acttctctta tcactacttt
     1561 ttaaagcctc aaatctctct aatatgtttg aaaaagctat tccagaacta tcctcttcct
     1621 ttgtcctttc agctccttca tttaatctat ctgtaattat gttcaaaaac tctccaattt
     1681 tcatataatc tcttccctta gaactaactc ctttagaatt ataaaatgtt actagtaatg
     1741 ttaaaatcac aaattgagac aaataataat ccttatcatt agcatttgat ctgcataata
     1801 ttttttttaa ttccccttta gaatatccta aaaagtcatt ttcttctcta ggaataaggt
     1861 acactactcc cccatacttt tttatgtaac attctgaaga ttcaccaagt tcttttacta
     1921 attcagttac attattattt tctgagtaac ttttatataa atcattttct tcttctttaa
     1981 gttcaccatg ctttaataaa taataaaaaa ttttattact tatttttatt tcttccatag
     2041 aataagccat ttttatcctc ctttatagct tcaaataaaa tatctgaaca gatgaaattt
     2101 ttaaattctc catcttcagt ttttactcct tcaagtttaa tatcatcctt atcaattaac
     2161 ttttttatat taatttcttt tatactatca agttcactat tattatctaa aacttctaat
     2221 atacttttat taagttgaaa ttcaatttct ccattttcaa tagcattctt tctttcttcc
     2281 ctaatttcat ttatatttat atttctgtgt ttaagcattt ctataataac ttctctaaag
     2341 atttcaacgg ttggaattaa ttttttcatt agttcattac tctcttttat taatccttga
     2401 atttctctaa gtgaaattcc tccctgtttc atggataatt ctaaaatcaa ttttattaca
     2461 ttattatatt ttttaagttt ttctattttc tttttattta tttcatcaat aaaatcactt
     2521 tcttcaaaag acaattcctc atcctcttca gaataatttt tatttatatt tttttgatat
     2581 tctaaagatt tatttatatt atacttttta ggcaactccc tctttaataa tggccttaaa
     2641 aagatatcta tatcctccaa tttattagga ttatctaata ttttatcgta aacctcattt
     2701 ctaaaattaa atctttcaat caatgccatc tttgatattg tttctagctc cataccataa
     2761 acatctttta aatcaaaatg ttttttcaat aatttttgat cttcatctat agttcttcct
     2821 aaataatttt gtattatttt taaattattt aaattttctt cctctttctt atttaatttc
     2881 ctaatgtgta tatcacgctc taaaaattca tttatcctaa gttcaaccat gcctctatga
     2941 attattaatt gtttttttat ttcatttaaa agattgagat ttcctgacat catcttttca
     3001 taatcttctg ttgtataact tagtggattc tccttaattt tccttattgc atcttcaatg
     3061 cttttaactc ttattctata ctggttaaat atatttttta tatcatcaac tgctttatca
     3121 taagaagcct tttctaaatg catcttaagc aaaatttcat gtatagttat ctttaaattt
     3181 tcttctactt ctaaggttga taaaataaat gaatacccat catcagttaa tgaatatact
     3241 actcttctaa catcatctat atacttcata ctatttttaa taaagcttat atttatatct
     3301 tcatactgga cctttttaaa attataagct ttaaaataca tggctttacc ttcatcacat
     3361 aaaatcgtat taactataaa ttcagccaac tctttacatt cttcataaga taattctttc
     3421 ttaaaatgaa tactgtttat ttcatcaata aaatacccta tattgtctat tgtacacata
     3481 tcatctttta aagcttcttc cattatataa agtaatactg caaagattaa attatcctgc
     3541 tcatatcctt ctgaaaatcc atactttttc caagtacctt ttgctatact atttctaaat
     3601 aataatgcat aagcacctat tcttttcatc cttatatgtt gaacatctaa aaaatcaaaa
     3661 tccattcttc ctcctataaa tctgttaagt taataatctc ttgtggaata tataaattat
     3721 tctctaatat cttcaatatt tttccattaa gtttttcact aaattcttgt aaaaatacat
     3781 tttctatatt cctattttga cctgccttgg ttttaggcaa attaatttcc atcatttctg
     3841 atttttctac cattttttca tatcctttta taaatggtct tatattatat tcttcagaaa
     3901 acatgtgata aaatttttca taaatcaata ttccttcata atctaaatca ccaaaataaa
     3961 agaatttatt atttttgtta gaaacatgat tttctacaga tactctaaag tctttaaacg
     4021 ctttccaaac attcttgcct gctccatata ttattgttgt tatttcctta cctaaaattt
     4081 tattgttgct tattaagtaa tttcttagtg tataatatgt atctttattt tctataatca
     4141 atacattttg aggagtattc ttacttcttg aataatatgc taaaggctct gaagtatcat
     4201 aataatttag cattcctaaa tcaataccta agttttttaa tattgatttc ccttcttctt
     4261 tttgcaaaaa cttctctctt ccccatattt gaaaagccct ttcattcatt gatactggat
     4321 aatttaaaaa ctcactttta tttataaaaa aatcatttaa ttgaagtata tattttctat
     4381 gatcaatata cttctctaaa tgatttctgt aatactcaat attaaattta ctaattaatt
     4441 tatattcaat ttcatctatt aattctttat tatcttcttt tatatcaatt atcgtatatc
     4501 ttttatataa agtagggctc ttaccatttt gtccactact aattataggt tttattttat
     4561 tctcacttat taactcatta acttttacat ataaatcaca ataagtatct attttaaata
     4621 atttttctaa ctcatctaat tctattcttt ttcttttaaa actacttaaa ctcaaaatct
     4681 tttctccttt ctcttataat taataatatt caaattgaat tttattgata ttattacccc
     4741 ctataaaatt atatcaaata aagaaatatt tatacaaatc caactctgtg ttatgatttg
     4801 ggtaatccat agtgattatc ctccgtatat tgtatttttt tgctattaaa tcataacaca
     4861 ggttaatcaa ccatggattt tttatttaat tcaatttcat tttacaacta agcactaatt
     4921 attatctaaa atttgttcaa acattttctt actataatca ccaatatcag agggatttat
     4981 catattattt ttttctttaa cagaattaaa gtaatccgct attaatcttt tatcaattat
     5041 tctatcagta cctattgaag ctgataaatc acctctagtt tctatccaag gcatctctgc
     5101 atgtgtaaat gcctctaata ccttaccact ataacaccct aaatttttta taacgctatc
     5161 tataataact ttctctgata ttgataaatc aaattgtttg ttaatagact cttctttaat
     5221 tggatcaaat ctgtattctt tgtatttata atatatattt ctaaaaacag gaccatgcac
     5281 ccatgcttca caattctctt caaatataaa ttttttcaaa aatgcataat aaaatccctg
     5341 aatataatat agtatttttt gtaatgcaag aggagtaata tcttcacatt cgcataataa
     5401 ataattaaca acagattcaa ttttactatt ctctaaattt tgttgttcac ttattaatat
     5461 ctccgttgct tctttgctct tatcatatgc tatgggtttt aatctatctt tattttcttc
     5521 taataattct aaataataac taggttcctt atatatcttt tctaatatag ctgaatattg
     5581 tttagttggc atatctccat cacaataacg actaaaagtc tgctctcccc atcctaataa
     5641 taaggataaa ggtcttttcc caatcttata tttattaggt atttctaata tatgctctaa
     5701 cgaaataata ttattttgtt ttctaaactc attatataat tgattcaaat tataatcatt
     5761 tatttcactc acaaatactt cattgttgca ctctttacaa atagcttttt ttccataata
     5821 tttataagtt tcacctttta acttcgatat catatattct tctttttcaa tataagaaac
     5881 atcatctcta caatactcac aaaatgtaat tttctcactc attttacatt cctcctaaaa
     5941 aatcccaata aattatctaa ataaatattc tattggctta tttctcttat gaaaagaaat
     6001 agtaactaat cttttttact atttctatat tctataatat taaatttaat atatatatat
     6061 caacaaactc ttcctcatca aaagcatcaa ataaatatct ttgatgacaa aatacataaa
     6121 gaacttcatg ttcatatcct tgatttgtat tattcaatga atgacaaaaa tcatttattt
     6181 ctatacttaa taaaatatct tttctttttt ttctcgtcta atctatattc attaatgaat
     6241 tgtatatttt ctaatctatt ttggttttgc gatattatgt attgattgtc ttttatgcaa
     6301 ccttttattt tatttaaaat gacttctact tcttctcttg tataattttg attataatgc
     6361 tctgacacct aatagacctc caaatttcat atcctttgtt attataatac tatattatat
     6421 acaggcactc aagattttat gtatcaatta gtacattttt atttgatatt agtattattt
     6481 ttaaaataaa ttatattaac tttaaaaata ataaaaagga ttctataaat gatacttcat
     6541 gtatagaacc cctttttatt caagtcaaat atactctatt ttactaagga tatttatatt
     6601 ttcactttaa attaaacaaa ttattccata taatctccct aacaacgctt gtatagagtc
     6661 atttaacact attttaaaga aataagaaaa ttacagaaac atctatcaga attatgaaga
     6721 tgctaaaaaa gcaatcttta aatacataga atgtttttat agtagtaaaa gactacattc
     6781 atctctttac ttttaatact actaatgaag tagaatttaa agctttaaat gcaacttaaa
     6841 taagttcgaa tttccatgtc caagatattc atacaaattc aatattcttc ataaatgtta
     6901 tttgaacatt ctttaaaaaa aatctttttc ttccaaagaa tatcagtttt atctttagct
     6961 atattataat cacctttttt caatctagtt attgatgctt ttggcaaatc aaaacataat
     7021 aaaaattcat aaataaattc actattttta tatgttttat tatctattaa attcttaatc
     7081 ttaacctcta tatcatttaa attcatattt atctcctaaa atatatttaa tcactattat
     7141 tttaacatat tattttatat atagtgatta tcattgataa atcatataaa gaacttaaaa
     7201 atcaatttga taaactataa tcaagagttt ctattgtcta gtaattttaa attaataagg
     7261 ttaccaaatc agctcattat tcttagacat actgaattca aattcaggta atactggata
     7321 catgctaaat tgtaaatcat taacctgttt aaaattttca gaaaagaatt tataatattt
     7381 agaatttctt aaaccatata taattaaatt tttcttacat cttgtaattg ctgtatatat
     7441 taattcttca tttacatttt catcagtatt ttcatctaaa attaatacaa gcgtatcaat
     7501 ttcccatcct ttaaagctgt gaatagtact tatttttaaa ttttcactca caactttaaa
     7561 agaatatttt ttatttcttc ttacttcatc aagtcttgat ttaagtagtt tactataata
     7621 attattattt ttaaaaacac aatttaaagt atcatattga ataatttcat ctactgaaca
     7681 ttttatactt ttatactctt cctctgtttc aaatgtaatt tcactctcta tattataact
     7741 atcccttact tccttttcta atgctcttaa attatctata ctttcaccta atatacaaat
     7801 ttcaccatac tctatattct tttttctaac gtattcttca atatccttaa atatatcttt
     7861 ataatcaatt atatttaaat aattaaatat tgaatcttga tttaaattat cacttaaatt
     7921 cttgtctatc tgatcaaaca tattaatttc ttcttcatct ggcactttga tatcatctaa
     7981 attatatcta tcttttaaaa attctttttg aaattcacca gctaatcttg aaaattctat
     8041 tccaattcta aatgttttat ttaattcatt ccatcttcct cgtatattag ttttaattct
     8101 tttatcttca tctaattcaa ccctattata tatattttgc ttctcatcag caaaaataac
     8161 aaattctcca ttattagcta aaaaatattt ttcaattata tcaaaccatc ttttatcata
     8221 atcttggcct tcatctataa aaattgcatt atatttcttt attttttctt taataacttc
     8281 tagctgttct ataattgttt cataatattt gtcttcttca taataattat cttctccata
     8341 atcaacatga gctattgttc ctccatattg atttatcttt tgatttatga aaaaatgaaa
     8401 atgatttatc tcaaatttac tccaatcaaa tttctttctc accatattta atctataatt
     8461 tatataattt cttaatgtta tgttaaatgt tagtataaga acttctgatt tagttctatt
     8521 taatgcattt actgctcttt atgctaatac taaagatttt ccagagccag caaccccttt
     8581 tattttaacc ttcttatttt ctaaactttc aattaaactt ttttgtttct tatcaaagct
     8641 tatacacgcc ccatcacctt ccaaatgtat tgctggattt ataaaattaa aaatttcatc
     8701 atatatctct gtatcaaaaa gattatattt tttatcttct ccccacctct taatatcttt
     8761 tacaatatta gaatcatatc cccatacatt tatatattta ttgaacttca taaatttact
     8821 taatggtata tgatgtttaa actcactttc tttaaaatgt tgaaaataca caccacaact
     8881 aacaacacca taatatttct tattaaatat tttcctttcc ataagctttt tactataaaa
     8941 ttcaaataaa ttgtttttac aagaatttac ttgatcaaat ggacttctaa atcttttatg
     9001 agtatttttc tcataaaaat atttttttgt atcattttcc tttttttcat aagaatattt
     9061 gcttaaatcc caatcactaa ttttaattat ataaattcca attctttttt tcattatcaa
     9121 tatatgaggt ctatcaccat ttaaattagg tttataatat atttcataac tattatctaa
     9181 atcttctttt agtttgttta ctatatttac atctgtattg tatctagttt cctctaatgt
     9241 aaacttatcc ataccattct tcttatttat ctatatatta cacatattct aattatagta
     9301 taatttttct aaaataaaaa tccataacat attaactctg cttatctaaa acaaaaaccc
     9361 tccaaaggct cttttctctg cctttagagg gtactatttt tttgattttt tattaatttt
     9421 tttgactttt tgtaaatgca agcacttttg tgactggttt taaaaaagtc cgtcaactcc
     9481 agaaccattg gtatcactga cttgcgactg actttgacga actttatata ttttccactc
     9541 actatatata tacaattaaa taaattgcat ttttttatac tatttttttt aatttcctat
     9601 atataaagag taaaaaaaat acgtcaatta tagcacagcc cttgatatca ctaaaaaagc
     9661 tctggtttca actctgtcat tagtccgtca caactcagtc acactaataa ttttaaactt
     9721 tacaattatt tcttaataag taaagcaaca ttttcaatat gtgctgtgtt tgggaacata
     9781 tccactggtt gaacttctaa agttttatag ccattctctt ccaatacttt taagtctctt
     9841 cctaaggtac ttggatcaca gcttacatat actatttttt tagcatctat atttgttatg
     9901 gcatctagta attttttatc gcatcctttt cttggtggat ctactactac cacatcagcc
     9961 tttactccct tatttattaa gtctggaatc accacttctg attctccaac gaagaattcc
    10021 acattctcta ctttattttc ctttgcattt atccaagcat tatctatggc ctgtggaatt
    10081 atttctactc cataaacttt tttagccttt tgtgatagga ataatgttat tgttcctgtt
    10141 ccacaatatg catcaaatac ctcttcattt cctgtaagat ttgcatattc taaagcctta
    10201 ccatataaaa cctctgtttg agtaggattt acttggaaga atgataatgg agatatgttg
    10261 aatctaaagt ctcctatata atctgatatt gtatcttctc cccatagagt tttactttct
    10321 aaacctaata ttacattagt tttctttgag tttatatttt gtataacact ctttattcca
    10381 ggaatatttt taacaattaa gtctacaaac tcttctttat gaggaaggtt ttctccattt
    10441 gtaactaaga caaccataac ctcattagtt gtaaaacctc ttcttatcat tatgtgtctt
    10501 actattccct tttcatcata ctcaccatct acattatatg gtcttatatt aaacttctct
    10561 atccattctc tagtaagttt aacaacctta tctcctattt catcttgaat taagcaagat
    10621 tccatgtcga taatatcatg acttcttgga gcaaagaatc ctattttaac ctctccatta
    10681 attaacccta ttggtaactg aaccttattt ctatatctcc aaggctcttc cattcctaaa
    10741 ggatatttaa ctatgctagg atctaacttt ccaatcttgc taacacagtc cttaactcta
    10801 tcccatttaa aatctaattg tgccttgtaa gaagcgtgct gaagtttaca tccaccacat
    10861 cttttataaa tagcacaagg tggattaact ctttcctctg aagcttctaa tacttccata
    10921 agttttccat aggcaaaatt tttattaacc ttaactattc ttactttaac cttttcgccc
    10981 tttaaagctc cctcaatgaa aattgggtat ttattatcaa ttttagctat tccttcaccc
    11041 tcatatcctt gagagattat atcaaaaata tattctttat tcttttcaac catccactaa
    11101 aactccctta tgtatattta atcatctatt ctctatggtt tatttaaaaa cctaaatttc
    11161 atttatatta gaataaatca ctttaatttt ctcttaatat aaaagctatt ttttaatact
    11221 atcctaaatt cagtattttt actaattatt attttagtta attacactaa aacacaaaat
    11281 gaactaaatc atcatattaa gttattatat aaacttaact aataactaac ttttaaagtt
    11341 tatcatatgg aataagcttt tacaacgcac tattaataag ctttctctac tagattaagt
    11401 taaagtactc ttaacttcct cacctattat ctttatgccc ttttctatct cttcctcaga
    11461 aagccttgta aagccaatcc ttaaggtatc tctcttctct cgccctttaa tattatgttc
    11521 ctctaaagaa aatactctgt tttctttttc taggtaaaga tcattatcta tataaaataa
    11581 atctccaggc ataaaaagaa ctcccctatc atagcacctt tttaaaagct ctcttgtatc
    11641 aacacctttt atttttaaga acatataaag tccaccttcg ccccaaattt ctgcatcttt
    11701 aatatatttt tctattaccc tcttagtaaa tttatatttt tccttataaa cttttctaac
    11761 tttcttaata tacttttcaa aggctccact tatcatgaaa tcataaagaa ttccttgatc
    11821 taagaaggag gtgtggatgt ttctacatct ttttacactt tctaatatcc ctattatttt
    11881 cttatctcca tatacccatc ctattcttaa acctggaaat aatattttag aaaaacttcc
    11941 tatgtatatt acaccattac tttctctatc taaggatgct aaaggaatag ttggagaact
    12001 ttcatataaa agctcctcat taaaaccatc ctctataata ggaataccag catcttttaa
    12061 aatattatag acctcttctc tctttttaat agatgccaca actcctgtgg ggttatgata
    12121 tgagggaatt aagtaagcaa atttaatctt ttcccccaat ttttcaattt ttttatttaa
    12181 atcctctatg tccatgccat ctttggtcat tttaacccca ataatattta gtttgtgagt
    12241 tttcattatt tttaaagctg tgttatgagt tggattttca caaagtatat aatttccatc
    12301 ttttgtaaat gaagaaagca tcatatctag tccctctgta aatccattag ttactattat
    12361 ttctttttcc tctgaataag cacccttttc tttcatataa tcctttaaat aatctataaa
    12421 aggcttatat cccacagcat aaccataatt aactatttta tgtttctcta aggcaattct
    12481 attagaaaag gatctcttta actcctcaat attaaaaaga tttcctggag gagctatact
    12541 tttaaaagaa atcattccct tttcccactt ctcttcactt tttataatat ctaaactttc
    12601 agctaatact ccatattcat tagttttata cttccaatca atattccact tgtcctctct
    12661 aatttcagag ttttcttttt taattgatga aactttagta gacttttcat tagaaaattc
    12721 tttttttaca aaagttcctt tgccttttat ggtttcaaca aagccttcac tttctaaaat
    12781 ttcataagca gacataaccg tatttcttcc tattgataaa atactgctaa tctcccttga
    12841 ggaaggcagt ttacttcctt ctattaaaac tcccctatta ataagctctc taatatacct
    12901 tgctatttgt atatacttct tctcattttc attaaaagtt aaatttttaa acataacttc
    12961 ctccaaagta aattcataaa aaacctttct tttaagataa aacttatttt taattacata
    13021 attttaaaaa tcaaataaaa tctctttttg atccacgctt tcaacattaa actaaataaa
    13081 ttctatcata tatgttttta ttcaaacaac tatatccatt catagattca atttccagta
    13141 taaataaaag catttttaca cattatatat actgttggta gttccaacaa agaggttctt
    13201 aatttaagga ggaatttatt atgaaacaca attcaagtat cggatgtaca gttaatgaat
    13261 gtaagtacca ttgtaatgag gacaattatt gtactctaaa caaaatcgaa gttgtaaaga
    13321 acactgctga ggcttgttct acagaatgta ctgattgtgg aagtttcgaa agaaaataat
    13381 aaattttcac tctaacacca aaaggaggct ataatttagc ctccttttca aaatttttat
    13441 gttgaattat attattttaa gcattatttc attaaatctc tcacccaaat tttacaactt
    13501 aatttttttc tattttaatt acataaatga agaaaataag ctttaatttc ttctaaaact
    13561 ttttaattac tattttaatt actgttacca ctttaattac ataagtggag caaatactct
    13621 tattatagat cttaaaattc tagtaggaac tttaacctta gcacaatctt ctaaagttat
    13681 tctttgacat ttttctaaag tttttaagaa atcatccttt atctgttcta ctgatttagt
    13741 tttatatagc caaactccac attcaaaatg aagatataaa cttctataat ccatatttat
    13801 agtacctact attcctattt catcatcaga gacaaaggtc tttgagtgaa taaatcctgg
    13861 agtatattca tatatcttaa caccagattc tataagctga ggataatagg cccttgtaac
    13921 tatatgagca taccatttgt cctcaatgtg aggagtaact attctaacat ctattccact
    13981 tttagcagca agagtaagtg ctgtaacaag ctcattatca ataataagat atggagtatt
    14041 tatataaaca tagtcctttg ccttatttat aatatttaaa tatacatttt cccctactat
    14101 ttcatcatct aaaggactat ctccataagg ctgaacatat ccatcacttt caaattcatc
    14161 ttcatgatga aaatatgggt aatatttttc atactgctct tttctttcac tatagaaatt
    14221 ccatatttga agaaacataa ctgttaagtt ccaaacagca tcccccttta acattattga
    14281 ggcatctttc caatgtccaa atctaacaat ttcatttatg tattcatcag ctaagtttat
    14341 tcctccagta aatccagtat gtccatctat aacagttatt tttctatgat cacgattatt
    14401 catccttaag gacaaaaggg ggacaaaagg attaaacacc atacatttaa ttcccttaga
    14461 ctctaaaact tcattatact tatatggtaa agtgcctaaa catcccatat catcataaac
    14521 gaatctaacc tctacccctt ctttaacctt ttcctctaat atttctaaaa tagagttcca
    14581 cataactcct tcatgtacta tgaaatattc cataaatatg tagtgctttg ccttttttaa
    14641 ctcttctttt aacctttcaa aaaacacctc tccaggagat aaatactcag ttgtagtatt
    14701 tttatatact ggaaactgag agtaatcagc tatgtacctt gattgattag ctatgctttt
    14761 atctaagttt tctagctcct ctaaaactgt tctatcatca tataataaat gtgccgtttc
    14821 atcataagaa gcttttattt gttttctaaa ctttttgctt gtcttatttc ccccaaatat
    14881 taaataaaat aatcctccaa agacaggaac taataataca ggaactgccc atgctaattt
    14941 ataggatgga ttatcttttc tgctaacaat ataaataaat actactatac taataaagat
    15001 aaacagaaaa tatacatata tgaaatcttc gcttactttc catattgccc ccataagtat
    15061 aacaaactgt atgaatataa gaaatccaac tataaatgct ctacttagta gttttttcag
    15121 aaacttttcc atttaatcct ccttgaaact tatgaatcca atttacctct ttaatttttt
    15181 atattaaaaa ctcttcttaa ctcttttaag aaatgatata ctaaataaat taatatctaa
    15241 tattaattta cgccatattt aattctaata gtaaaactat tatctctaag tactacataa
    15301 tattagtcca ttaactccca tccctttata gctaccttgt aatagagcca tctaggtgta
    15361 aacttactga caaaatataa aaatttattg caaaatccag gaataactat tacctttaat
    15421 ttcttattta tattttttaa gcttatttcc actacttctc ttgtactcat ccatcttaca
    15481 aagcctttat tttgcaattt actctcttcc atgtttaatt tgctatgaaa atctgttcta
    15541 gtaaatccag gacaaaggca ttgaaccttt atattattct ctaaaagttc catggctaaa
    15601 cattgagtaa aacttattaa gaaagccttt gtagaacaat acattgtgga acttggaaat
    15661 acgttaaatc cagccataga actaacattt attatgtatc ccttattatt ctctttcatc
    15721 ttcttgctta agctatgaca aagcttcata gtaactagca tatgaacctt tgccatatca
    15781 tattgcttac tatattcatt ccttgtaaag gaatcatcag ctccatatcc tgcattattc
    15841 actaaaaatt ctatattatc ttttttctct aatcttttta taaaatcatc aagtttatta
    15901 tcatcagtta aatctaaaat ttctacatgt gtcttaacct taaaattatt ctctatctct
    15961 tctgcaacag ctttaatttt ctcgactctt ctacctacta atattaaatc atatcctttt
    16021 ttagctaatt gaaaagcata ttctttccct attccactag ttgctccagt aattacagct
    16081 atcttaccta actcattcat tttactcccc cttatattgc acaaataatt ttgaagtaat
    16141 ttatattaat ttcttaagaa tatattttac catataatta aaaagctaga acctaaaatt
    16201 taatatttta gttctagctt ttaataatta ttttttataa aaagtttcac attcagttga
    16261 agtagatgtt gtagcttctg gtccaaaaac ttgtatatca gtagaagtac aagtcattcc
    16321 aacattatat gtacaattca tagcataaca ttttatctta tgatctacat cttcctgact
    16381 atctagtgct tgcattattg ttccaacata attagtatta aatgcacttt taattgcatt
    16441 tacaaaggtt cttggtgcaa aggtattgca acaggtttct tgtttattct ctgcactctc
    16501 tccatcaact tcaatggtca aagctgaaca cattccatta acatagttaa cacaattatg
    16561 agctgaacat gctaatcttg tcatattcaa atcctcctca aaatattatt tgaggttatt
    16621 ttttgtattt atattaaatt tatactataa aactatatta atctaataat tttaaataaa
    16681 aaaagaagta ctcgatggta tcagtcatca agtacttttt catcttggaa tcagttgcca
    16741 tttgattcta cttatattat ttcataagca ctatctaata tacactattt tcttttactt
    16801 gtcataaaaa tattgttaac tttttgttaa ctatatattt ttatagattt tatttaatat
    16861 aatttatttt tatctttatt ctattttaaa atatttacat tt
//
LOCUS       DAEVGI010000041        16750 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEVGI010000041 DAEVGI010000000
VERSION     DAEVGI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 16750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16750
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.041"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(443..1126)
                     /locus_tag="K8M77_001176"
     CDS             complement(443..1126)
                     /locus_tag="K8M77_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBI7085683.1"
                     /translation="MSLNTKNNLYREILDRIDRGGNWEMIVVDGVVGVGKSTLMNILA
                     ERGLIAYEEPVVDNPILEKFYYDRNRYSFPLQVFFLNKRFKHIKEAGLVENSVMDRSI
                     YGDVIFAKMLCEKGEMTVEEFELYKELLENMLEHVKAPKLMVYLEISVDEAMKRIQKR
                     GRDYEQVVEREYWEKLNNEYREYFENYNVSPILRINVDELDFENIPEDREYVLNLIDE
                     KLSELENNK"
     gene            complement(1146..2084)
                     /locus_tag="K8M77_001177"
     CDS             complement(1146..2084)
                     /locus_tag="K8M77_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085684.1"
                     /translation="MSFDKNVFIEAIRGNDREYVVKDKVGIILGRFFLKDLDSKNKKV
                     DVKFKFYKEDNYKLMKETLRKILKVLFAEKDIYKINLFVSDIKNFNAYLDLGFMLEGI
                     LSDNVCNNGIQKDEYIMGININDFNDKVKLVQFQLNTERLALRNLTPENTEEMLDYYI
                     RNEEHLRQYEPTRDSSFYTYEGQKEILTESFRQFIDGTSIDLGIFKDEKLIGKIKLSN
                     IVYGILRNAFVGYSIDKEHQGKGYMKEALNTVCSYAFEEMGLHRLEASTLMDNSRSQG
                     VLKACGFNELGISEKYLYINGEWRDHKIFYKVNDDL"
     gene            complement(2100..3275)
                     /locus_tag="K8M77_001178"
     CDS             complement(2100..3275)
                     /locus_tag="K8M77_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7085685.1"
                     /translation="MNSKKQNYVALAFLFIMMILAAIVENTKGIFIPVFKQEFGVNDN
                     TISNLLISTSAAYMVLTFIGGMLCEKFGQKKVFLAGLMVIFLSLIFLSFTKNYLMLYT
                     GMVISSAGIAMVAISCNTVLPILALTAQNVIMNVMHACYGLGSSIGQGLFGSLTARGV
                     NWRTMYFFVGLVYLAVFICFIFVKIPRTEIVKEKDKMTIIEALSNKVIVAYMLALGLY
                     VFTEQGISNWFVNYMKYGYNINEQKAGMYLSLFFAIFTIGRLFGGFVVEKRGYFNILY
                     KTLIIGAALCILGLLLGRNGMVIISVSGLFFSITFPTTVLTISKVFDKNVAYITGIVI
                     TSASLVGMILNKAIGLLNESVGPDKAFYIIPASAILSAILMFYLYLNTKKRLVKQKI"
     gene            complement(3567..4328)
                     /locus_tag="K8M77_001179"
     CDS             complement(3567..4328)
                     /locus_tag="K8M77_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBI7085686.1"
                     /translation="MLQHSLSRTELLIGKDALDKLAKSKVMVFGVGGVGSFTVEALAR
                     AGVGNLILVDDDTVCLTNLNRQIHATYKTISKNKVEVMKERVLSVNRNCNVETIQVFV
                     TPDNLEEIIPDDVDYVVDAIDTVSAKIALAVYCEQKGIKLMSSMGTGNKLNPAEFKVA
                     DIYNTKVCPLAKVMRYELRKRGVKKLKVVYSEEMPRKPKVEDVVTCKTGCVCTGGTKK
                     CSAKRQIPGSVSFVPPVAGMIIASEVVKDLIKEYI"
     gene            4704..5399
                     /locus_tag="K8M77_001180"
     CDS             4704..5399
                     /locus_tag="K8M77_001180"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBI7085687.1"
                     /translation="MTLGIIAAMSEELEILLKDMEVKSKETKANMTFHQGTLWGQEVV
                     AVVCGIGKVNAAVCTQILISEYKVDKVINVGVAGGIGMEIYPGDVVIGNSLVQYDMDT
                     SAFGDPIGQIPRLDTFDFKCSQELVDLCEEACKENEEIKSFTGRIVTGDQFVASVEKV
                     KWLEEEFGASACEMEGGSIAHVCYLNNIPFLVIRSISDNANNGAHMDFAEFTAIGVKN
                     STSILKSMILKMA"
     gene            complement(5455..6711)
                     /locus_tag="K8M77_001181"
     CDS             complement(5455..6711)
                     /locus_tag="K8M77_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBI7085688.1"
                     /translation="MVNIKAFKALRPRKDLVEKVAALPYDVLSRNEAKEEVKNNEFSF
                     LNIDKAETYFPENISEYSEEVYKKAGENLEKFIQNNIFIKEEKDCLYIYELDLDGKSQ
                     RGIVSCLSVDDYLNNTIKKHEFTRAKKEEDRVKHIKACNAQTGPIFLTYRDSEKISNI
                     VSKIVLKKPLYDFISDDGVLHRVWKIDDKSIIDEIIKEFKEINSIYIADGHHRLEAAV
                     KVCLERREKGECLPEDECNYFLGVLFPKTELTVIDYNRVVRDLNGLSEEEFLLKLEEK
                     FQITKLNKEDNPKPKHKGEFGMCINGDWFKLEYKDKNEISLIEKTKIDLVKNLDASIL
                     QDNILEPILGIKDVRNDERIDFVGGIRGLKELERRVSEDMKVAFALYPTSLEEVMKIS
                     DKGLIMPPKSTWFEPKLRSGIFIHKI"
     gene            complement(6845..7750)
                     /locus_tag="K8M77_001182"
     CDS             complement(6845..7750)
                     /locus_tag="K8M77_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBI7085689.1"
                     /translation="MLRILLNDGLDKKAISNLEMLGCDVDTNHYDIEDLKEKIKEVDC
                     IVIRSATKIRRELIDEAIKGGKLKLIIRGGVGLDNIDVKYAEQNGIKVRNTPNASSSS
                     VAEIILAHMFSLARFLNQSNITMKAGLWKKKEYVGVELEGKTLGIIGMGRIGSELAKK
                     CTALGMKIIYFDLMDIKNIDNNYRKVEFDELLRESDFISINISGTKSIIGSEELKKVK
                     KGAFIINTSRGKALDEDAIITSLNDGTLGGIGLDVFLEEPSKNLELIKHPKVSLTPHI
                     GASTKEAQMKIGEEVINIIKEEMYK"
     gene            complement(7820..8896)
                     /locus_tag="K8M77_001183"
     CDS             complement(7820..8896)
                     /locus_tag="K8M77_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBI7085690.1"
                     /translation="MHKKLFIPGPVEVREEVLEKMATPMIGHRSKEASQLQRRISDNL
                     RKLFFTNNEILLSTTSGSGLMEGAIRSCTLKKAAVFSVGAFGDRWYEMGISNNVPVDK
                     FEVPWGKSINVDLIDSVLKTGQYDLITVTHNETSTGVMNDLEAISKVIRKYPEVIFCV
                     DAVSSAGGSKIEVDKLNIDICITSSQKALGLPPGISICTFSEKARERAEKVKFRGTYL
                     DLLALYKYIKKKDYQYPSTPSISHMYALDYQLDYILNVEGLENRFNRHIEMAKIVREW
                     AEKYFEIYPEKGYYSNTLTTIKNNKNIDIGALNKELGKRGFQISNGYGKLKDKTFRIA
                     HMADCQVKDIKELLSNINEILELE"
     gene            complement(9060..9242)
                     /locus_tag="K8M77_001184"
     CDS             complement(9060..9242)
                     /locus_tag="K8M77_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085691.1"
                     /translation="MAISEEFIDIAENCVDYSARYREAVSSMNSCKSCLNCNEFHSGT
                     CEKEMENKFNILQDMI"
     gene            complement(9311..9490)
                     /locus_tag="K8M77_001185"
     CDS             complement(9311..9490)
                     /locus_tag="K8M77_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085692.1"
                     /translation="MAVGTSINCKYCIDFKNDRCDGTEKNCLCRLCPRNLGQCICVKY
                     CRETESVLFWNDEEE"
     gene            complement(9654..10784)
                     /locus_tag="K8M77_001186"
     CDS             complement(9654..10784)
                     /locus_tag="K8M77_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085693.1"
                     /translation="MKSNNLILDKYKSRGIRGLIVLGIAYFFLMGNYSMIRYFFQAGQ
                     GDLDISIINKCFSMGSVDKFLLFLFLVWSVGFLFQDKEKELKENLAVGEENTKKYFFS
                     KVILIYGVSLIPMLINIFIKLIFYIPYRNSFSLDKLIISFLYFIALGLLFTSIIFIMN
                     LIVKDRMFAGVFPIFFMEGLILIFSVSELLISDKLIWVRNSLGFIGEKVLLIFNLLNL
                     NFDTNSFYLGRQFIFIIILVLISVLLIYLSRLILKIMNFKYLYRPYFFEIPRYFIYIS
                     MSILIGFVFVHAVGYLIIMFIPSVSYVNGTFMVNIASLISMVLLFIFLDVLYKRKILN
                     KDLLEDDIKVCNEIQPCIGDFVILDKTTGFLDNKTIDKEENF"
     gene            complement(11060..11338)
                     /locus_tag="K8M77_001187"
     CDS             complement(11060..11338)
                     /locus_tag="K8M77_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HBI7085694.1"
                     /translation="MDIGALSLDDIILLIVGVLVAILILRLIFGSIGKIIGLVINSVF
                     GAILLMIVNYFGAYFGITIGINLLTALIAGILGIPGVVLMILFQVFVR"
     gene            complement(11354..11632)
                     /locus_tag="K8M77_001188"
     CDS             complement(11354..11632)
                     /locus_tag="K8M77_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HBI7085695.1"
                     /translation="MDKKKISDYLIKKIDYSNEDEEILKAIEQTILEMEVARSAFQNA
                     CDDKLIESLIYKEQDINARYEYLIREAKKRGIKVSMEHIFKNARISII"
     gene            complement(11858..12454)
                     /gene="recR"
                     /locus_tag="K8M77_001189"
     CDS             complement(11858..12454)
                     /gene="recR"
                     /locus_tag="K8M77_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBI7085696.1"
                     /translation="MEFYPVAIEKLIEEFAKLPSIGKKSAQRLTLHILNLPKDEVEEF
                     ANALVKARGTIKYCSVCGNFTDTDPCAICSNPNREKDIICVVEQPKDIMTMEKVKEFN
                     GLYHVLHGTISPMQGRGPQDIRIRELVARMSGDVKEVIVATNPTIEGEATAMYISKIL
                     KPLDVKVTRIAAGIPVGGDLEYADEVTLSKALEGRTVI"
     gene            complement(12552..12890)
                     /locus_tag="K8M77_001190"
     CDS             complement(12552..12890)
                     /locus_tag="K8M77_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBI7085697.1"
                     /translation="MARGGFPGGMGNMNNLMKQAQMLQKQMQSMQEEIEASEFEGSAG
                     GGAVVAKVNGKKELIALNIKPEVVDPDDVEMLEDLVFSAVKQALEKASEETSEKMGKL
                     TGGMGMPGLF"
     gene            complement(13002..14645)
                     /gene="dnaX"
                     /locus_tag="K8M77_001191"
     CDS             complement(13002..14645)
                     /gene="dnaX"
                     /locus_tag="K8M77_001191"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBI7085698.1"
                     /translation="MAYKALYREWRPQTFNDVVGQEHITTTLKNQIINERTAHAYLFC
                     GTRGTGKTSTAKVMAKALNCLDLKDGEPCNQCEMCKKINEGLAIDITELDAASNNGVD
                     NIRDIIDDVQYPPQEARFKVYIMDEVHMLSIGAVNAFLKTLEEPPKNVVFILATTDPQ
                     KLPITILSRCQRFDFKRISRKDITGRLRKIVTEQGIFCDDKSLDLVARVSDGAMRDSL
                     SILDQAISIGDGKVDYDELVSMLGLVTNDNLFKIVDSIIKRDIESAMEIIEELVLTGK
                     DMTLFIKDLIVHYRNLLMIKVTSNPEEVLDTAEENIEKLKVQGAKLKVEEIMRCIRIV
                     QECDEEAKLSKQARVYLELAVIKMCKIEYDTSKEVMLARINKLEQAIRSGNIQVNTNL
                     EEVKSQVSNEASNNKTLNNKQQNVIKSSNNYEVVNENSTLTPEYLRKVWKDILETFKS
                     RGKYMVYIALAKGISKVSCNKGVVEFRYTYEYRHNKERLEKHDFSKIVNDIFSEVLKE
                     PVRVVYSVEQGTEEEKAEEADLEARLSGIMGDGLEIVEE"
     gene            14836..15321
                     /locus_tag="K8M77_001192"
     CDS             14836..15321
                     /locus_tag="K8M77_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBI7085699.1"
                     /translation="MFSIIVAKSINNIIGVNNKMPWNIPSDLKRFKELTMGKKILMGR
                     KTFESLPFVLPGRTHLVLTTKKDFSYNHKDVIIYNDLDKLISEYKDSMEEIFVIGGGK
                     IYSEFLKYASKLYITEVLKEYKGDTYFPKVDYSKWIKTYESSIYEENATKFKYVIYEI
                     L"
     gene            complement(15397..16041)
                     /locus_tag="K8M77_001193"
     CDS             complement(15397..16041)
                     /locus_tag="K8M77_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI7085700.1"
                     /translation="MGNRNVVEISNLTKDIKGTKVLNKVNLQLESGKAYGIVGRNGSG
                     KSMLFKAICGLINTTEGSITVFDKVIKNGSFPDNVGMIIENPGFLLQYSAFKNLKILA
                     SINNKISDERIKETIALVGLDPEDKKIVKKFSLGMKQRLGIAQALMENPKLLILDEPM
                     NGLDSEGVKQIREILLKLKSNSVTILLASHNSDDIDEICDCVYTMENGVLSARI"
     gene            complement(16069..>16750)
                     /locus_tag="K8M77_001194"
     CDS             complement(16069..>16750)
                     /locus_tag="K8M77_001194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085701.1"
                     /translation="IVIFIYVFSVKINNYDSNFSFNEILEVIFAGPINLDNISEIFTW
                     TFYQAFLIYIIGNYIFNELSTRSIYVIPRLGNKQYWNICLQITCFLACIIYFTLGTFI
                     EFIFTAILNKGFNFSNIIDTLQIVVLLSLSSYYAITVYIFNIMVTKSHIKSFLILIVE
                     FYVSISIGNIFNIDKFIPLNQGILSKHIIYNFSYNWSFIFLGILIFINLVIINRLIVK
                     RDLLDITY"
BASE COUNT         5689 a         2931 c         1579 g         6551 t
ORIGIN      
        1 tttattttat catcaccttc gaactttgtc aacacttttt ttaaaaaact ttttaagttg
       61 ttttctttca acttattgtc tttttttgtt gcccgtcctt agtgacaggt attatcttaa
      121 caccatttct ttaatttaat ttaaatttta ctttagtttt tgaaaaacat ccaaactaat
      181 cttttaaatc cttttatttt ctctgtatat catatattga cacactagga aaagtttata
      241 gatattatat tcaattacta ttcatttatg gatagttttg tatgtttaac ccttgtaaaa
      301 acctaaatta ataataaatc atttatcata atcacccttt tttctttaaa atattcatat
      361 aattactaaa cttaaataaa aaaatacttt aataatataa aaaagaagag gatatttagt
      421 tattctcttc ttttaaataa acttatttat tattttctaa ttcgcttaat ttctcatcta
      481 taagatttaa aacatattct ctatcttctg gtatattttc aaaatctaac tcatcaacat
      541 ttattcttaa tatgggagag acattatagt tttcgaaata ctctctatac tcattattaa
      601 gcttctccca atattctctt tcaactacct gctcgtaatc tctacctctt ttttgaattc
      661 ttttcatagc ttcatctact gatatttcta gatataccat taattttggt gctttaacat
      721 gctctagcat attttctaaa agctccttat ataattcaaa ctcttcaact gtcatttcgc
      781 ccttttcaca tagcatctta gcaaatataa catctccata tatacttcta tccataactg
      841 aattctctac aagtccagct tcttttatat gcttgaatct tttatttaaa aagaatactt
      901 gaagtggaaa agaatatcta ttcctatcat aatagaattt ttctaatata ggattgtcca
      961 caacaggttc ctcataagct attaatcctc tctctgctaa gatgttcatt aaagtactct
     1021 tacctactcc aacaactcca tctacaacta tcatttccca atttcctcct ctatctatcc
     1081 tatctagtat ctctctgtaa agattatttt tcgtatttag agacaatatt gttcctcctt
     1141 aaaccttata agtcatcgtt caccttataa aatattttat gatctctcca ttcaccatta
     1201 atgtataaat atttttcact aatacctaat tcattaaatc cacaagcttt taatactcct
     1261 tgagatctag aattatccat taatgttgaa gcctctagtc tatgtaaccc catttcttca
     1321 aaagcataac tacatactgt attaagtgct tctttcatat aaccctttcc ttgatgctcc
     1381 ttatctatgg agtatccaac aaaggcattt cttaatattc catagactat attagataat
     1441 tttatttttc ctattagttt ttcatcttta aatataccta aatcaattga agttccatct
     1501 ataaactgtc taaaactctc tgttaaaatt tctttttgtc cttcataagt ataaaaactg
     1561 ctatctctag taggctcata ctgtcttaaa tgctcttcat ttcttatata ataatcaagc
     1621 atttcttctg tattctctgg agttaaattt ctaagggcta atctttcagt atttaattga
     1681 aactgaacta atttaacctt atcattaaag tcatttatat ttatccccat aatatattca
     1741 tctttctgaa taccattgtt acatacatta tctgataata taccctcaag cataaaacct
     1801 aaatctaaat aagcattaaa gttttttata tccgaaacaa aaagattaat tttatatatg
     1861 tctttttcag caaataaaac ctttaaaatc tttcttaaag tctctttcat aagtttatag
     1921 ttatcttctt tgtaaaattt aaatttaaca tcaacttttt tatttttact atctaaatcc
     1981 ttcaagaaaa atctacctag tattatgcca actttatctt ttacaacgta ttccctatca
     2041 tttcctctta ttgcttctat aaatacattc ttatcaaaag acatactcta ctctccaatt
     2101 tatatttttt gctttactag cctcttctta gtatttaagt ataagtagaa cattaaaatg
     2161 gcagataaaa ttgcacttgc aggtataata taaaatgcct tatctggtcc aacactttca
     2221 tttaacaatc caatagcctt gttaagaatc ataccaacta aactagctga tgttattaca
     2281 atacctgtaa tatatgcaac attcttatca aaaaccttac taatagttaa aactgtagtt
     2341 gggaatgtta ttgagaagaa taatcctgat acagatatta tcaccatacc gtttctacct
     2401 aaaagtaaac ctaagataca aagtgcagct cctattatta atgttttata taatatattg
     2461 aaatatcctc ttttttctac tacaaatcca ccaaatagtc ttcctatggt gaatatagca
     2521 aaaaatagtg ataaatacat tcctgccttt tgctcattta tattataacc atacttcatg
     2581 taatttacga accaatttga tataccttgc tctgtaaata catataatcc taaggcaagc
     2641 atataggcaa ctattacctt atttgataaa gcttctatta tagtcatctt atctttttct
     2701 ttaactattt ctgttctagg tattttaacg aatataaagc atatgaatac tgctaagtaa
     2761 actaaaccta caaagaaata catagttctc cagttaactc ctcttgcagt taatgaacca
     2821 aataatcctt ggccaattga agagcctaac ccgtagcaag catgcataac attcattatt
     2881 acgttttgtg ctgttaatgc taatatagga agaactgtgt tacatgatat tgcaaccatt
     2941 gctataccag cactacttat taccattcct gtatataaca ttaaatagtt ttttgtaaaa
     3001 gataaaaata taagtgataa aaatatcacc atcaatccag ctaaaaatac ctttttctgt
     3061 ccaaactttt cgcaaagcat tccacctata aatgttaata ccatataagc agctgaagta
     3121 gaaataagta agttacttat agtattatcg ttaactccaa attcctgttt aaatactggt
     3181 atgaatatac ccttagtatt ttcaactata gctgctaaaa tcatcattat aaatagaaaa
     3241 gctaatgcaa cataattttg ctttttactg ttcattatta cttctcctta ttatttaaaa
     3301 taaataaaac tttattagat ctatattatt aaaatatact actttaaacc ataaaaaata
     3361 tataatctat agtagttatt tataaaaaaa caaaaacata aaaattatac cataaaaaca
     3421 ttgaatagtg cttaattatg tatacttact ctacataatt aaaaacttct ttatatttta
     3481 aaatcaaaag ctttataaat agaaaaagag cttatatcaa aataattttt attatcatat
     3541 tttgataaag cccaaatttc tctatattaa atatattctt ttattaaatc tttaaccact
     3601 tctgatgcta ttatcatacc agctactgga ggtacaaaag atacacttcc tggtatttgt
     3661 cttttagctg aacatttttt agttccacca gtacaaacac atcctgtttt gcaagtaact
     3721 acatcttcaa cctttggttt tctaggcatt tcttctgaat aaacaacctt taacttctta
     3781 actcctcttt ttctaagttc atatctcata accttagcta gtggacatac ctttgtattg
     3841 tatatatcag ctactttaaa ctctgctgga tttaatttgt ttccagttcc cattgaactc
     3901 ataagtttta ttcccttttg ttcacaataa acagcaagag ctatcttagc tgaaacagta
     3961 tcaatagcat ctaccacata atctacatca tctggtatta tttcttctaa attatctgga
     4021 gtaacaaaaa cttgtatagt ctccacattg caatttctat ttactgaaag aactctttcc
     4081 ttcataactt ctactttgtt tttacttata gttttatatg tagcatgtat ttgtctgttt
     4141 aaatttgtta aacaaacagt atcatcatca actaaaatta agtttcctac tcctgctcta
     4201 gctaaagctt ctacagtaaa gcttcctact ccaccaactc caaataccat aactttgctc
     4261 tttgctaatt tatctaaagc atctttacct attagtaatt ctgttcttga caatgaatgt
     4321 tgtaacataa aatttctctc cttcttaagt taataaattt cccctatgtt aatcctctat
     4381 aatatattct aaaataaata tctcaaaatt ataatgttta ttcttatact taataaacac
     4441 acaaattcat taatattata tacattctac ttaatataaa taaagttttt catttatatt
     4501 aacatataat tcctttaaaa acttatttct acctagaaaa ttcatataaa atttcctgga
     4561 ttaagcccta atttaggtaa attttaatta ataataaaat tataaaatgt gctttcaaat
     4621 ctatttactt tttaaatttc tatgatatga tcttttttta atatataata actttatgaa
     4681 tttaatttga ggaggaattt aaaatgactt taggaataat cgccgctatg agcgaagaat
     4741 tagaaattct tttaaaagac atggaagtaa aatctaaaga aactaaggct aatatgactt
     4801 tccaccaagg tacgctttgg ggtcaagagg ttgtagccgt tgtatgtggt ataggtaagg
     4861 ttaatgctgc cgtttgtact caaatattaa taagtgaata caaagttgat aaggttataa
     4921 acgttggtgt tgctggtggt ataggcatgg aaatatatcc tggggatgtt gttataggta
     4981 actcattagt tcaatacgat atggacacat ctgcctttgg agatcctata ggacaaatac
     5041 caagattaga tacctttgat tttaaatgtt cacaagaatt agttgacctt tgcgaagaag
     5101 cttgtaaaga aaatgaagaa ataaagagct tcactggaag aatagttact ggtgaccaat
     5161 ttgttgctag tgtagaaaag gttaaatggt tagaagagga atttggagct tctgcttgtg
     5221 aaatggaagg tggaagtata gctcacgttt gttacttaaa caatattcca ttccttgtta
     5281 taagatcaat atctgataat gcaaataatg gagcacatat ggacttcgct gagtttacag
     5341 ctataggagt taaaaactct acttcaatat taaaatcaat gattttaaaa atggcttaat
     5401 ataaaaggat attatccaaa ataagtttta ttatattttg gataatatct ctttttatat
     5461 tttatgaatg aatattccac ttctaagctt aggctcaaac catgttgatt ttggtggcat
     5521 tattaacccc ttatcagaaa ttttcattac ctcttctaga cttgttggat aaagagcaaa
     5581 agcaactttc atatcctctg atactcttct ttctaattct ttaagccctc ttatacctcc
     5641 aacaaaatct attctctcat catttcttac atcttttatt cctaatatag gctctaaaat
     5701 attatcttga agtatagaag catctaaatt tttaactaaa tcaatttttg ttttttctat
     5761 tagtgaaatt tcatttttat ctttatactc taatttgaac caatctccat taatacacat
     5821 cccaaactca cctttatgtt taggttttgg attatcttcc ttatttaatt tagttatttg
     5881 aaatttttct tctaatttca ataaaaattc ctcttcactt aatccattta aatctctaac
     5941 tactctgtta taatctataa ctgtaagctc tgttttagga aataaaactc ctaagaaata
     6001 attacattca tcctcaggta agcattctcc cttttctctt ctttctaaac atacttttac
     6061 agctgcttct aacctatgat gcccatctgc tatatatatt gaattaattt ctttaaactc
     6121 ttttattatt tcatctataa tagatttatc atctatcttc caaactctat gcaaaactcc
     6181 atcatcagaa ataaaatcat ataaaggctt ttttaaaact atttttgaaa ctatattact
     6241 tattttttct gaatctctat aggttaaaaa tattggtcct gtttgagcat tacaagcttt
     6301 tatatgtttt actctatctt cttctttttt tgctctagta aattcatgtt tttttattgt
     6361 gttatttaaa taatcatcaa cagataagca tgaaacaatt cctctttgtg attttccatc
     6421 taaatctagc tcataaatat ataaacaatc tttttcttct tttataaaaa tattattttg
     6481 aataaatttt tccaaattct ctcccgcttt tttataaacc tcttcagaat actcactaat
     6541 attttctgga aaataagttt cagctttatc aatatttaaa aaggaaaact cattgttttt
     6601 tacttcctct tttgcttcat tcctactcaa aacatcgtat ggtaatgcag caactttttc
     6661 tactaagtcc tttcttggtc ttaaagcttt aaaagctttt atattaacca tattcctcac
     6721 gctccctcta aaaattaatc cctattttaa ttattattag attatattca tatataaaat
     6781 agggcttttt tcttttttat ctaaagttta atctttaaat ctacagagtt atatatttta
     6841 aattttactt atacatctct tcttttatta tatttataac ttcctcccct attttcatct
     6901 gagcctcttt tgttgaagct ccaatatgag gagttaaact tactttagga tgctttatta
     6961 attctaaatt tttacttggc tcttctaaga atacatctag tccaatgcca cctaaggttc
     7021 catcatttaa actagttatt attgcatcct catctagtgc ttttccccta gaagtattta
     7081 ttataaaggc tcccttttta acctttttta attcttcact tccaattata ctcttagttc
     7141 cagaaatatt tatagaaata aaatctgatt ctcttaataa ctcatcaaat tctacttttc
     7201 tataattatt gtctatattc ttaatatcca tcagatcaaa gtatattatt ttcataccta
     7261 aagctgtaca tttcttagct aattcagatc ctattcttcc catacctata attccaagag
     7321 tttttccctc aagctcaaca ccaacatact ctttcttttt ccaaagacca gctttcatag
     7381 ttatattaga ttgatttaaa aatcttgcta aagagaacat atgggctaat ataatctctg
     7441 caactgatga agaacttgca ttaggagtat ttcttacttt aattccattt tgttcagcat
     7501 atttaacatc tatattatca agtcctactc cacctcttat aattaatttt aattttccac
     7561 ctttaatcgc ttcatctatt aattctcttc taatttttgt ggcagacctt ataactatac
     7621 agtctacttc ttttattttt tcttttaaat cctctatatc ataatgatta gtatcaacat
     7681 cacaacccag catttccaaa ttgctaatag cctttttatc taatccatca tttaaaagta
     7741 ttcttaacat atcaaaatag cccccttaat aaattattta gtataaaaga aacccacact
     7801 aatcagtgtg ggcttagatt tattccaatt ctaaaatttc atttatatta cttaataatt
     7861 cttttatatc tttaacttgg cagtcagcca tgtgtgctat tctaaatgtc ttatccttta
     7921 gctttccata cccattagat atctgaaatc ctctttttcc aagttcctta tttaaagccc
     7981 caatatctat atttttatta ttttttatgg ttgtaagggt atttgaatag tacccctttt
     8041 caggataaat ttcaaagtat ttttctgccc attctctaac tattttagcc atttcaatat
     8101 gtctattgaa tctattttct agcccttcta catttaatat ataatctaat tggtaatcta
     8161 aagcatacat atgtgaaata gaaggcgttg atggatattg ataatccttc tttttaatat
     8221 atttatataa agctaataaa tctaaatatg ttcctctaaa cttaactttt tctgctcttt
     8281 ccctagcttt ttctgaaaat gtacatattg atattcctgg aggaagccct aaagcttttt
     8341 gtgaagaggt aatacatata tctatattaa gtttatccac ctctatttta cttccacctg
     8401 cagaactaac tgcatcaaca cagaatataa cctctggata ttttcttata actttgctta
     8461 ttgcttctaa atcattcata acccctgtag atgtttcatt atgagttact gttattaaat
     8521 catactgtcc agttttaaga actgaatcaa ttaaatctac attaatactt tttccccaag
     8581 gaacttcaaa tttatccaca ggaacattat tacttatccc catttcatac catctatccc
     8641 caaaagcccc cacagaaaat acagcagcct tctttaaagt acaacttctt atagctccct
     8701 ccattaatcc acttcctgaa gttgttgata aaagtatttc attattagta aaaaataatt
     8761 tccttaaatt atcgcttatt cttctttgta attgagatgc ctctttactt ctatgtccaa
     8821 tcataggagt agccattttt tctaatactt cctctctgac ttccacaggc cctggtataa
     8881 ataacttttt atgcacataa tcccctcctt cactttaaaa attattttat ttattagtat
     8941 ataataaatt ttcttcattg gtgatacaaa aataaataaa aataattata aattgccttt
     9001 ttagaaataa aaaaagacct atatcaaaaa aatattgatt aaggtctttc aaatataaat
     9061 taaatcatat cctgtaaaat gttgaattta ttttccattt ccttttcaca agttccacta
     9121 tgaaactcat tgcaatttaa acagctttta caagaattca ttgaagatac tgcctctcta
     9181 tacctagctg aataatcaac acaattttct gctatatcta taaattcttc actaatagcc
     9241 atacaaattc tcctttctca gaacaatact atatgcttag tattttctaa gaaagttttt
     9301 ttatactaat ttattcctct tcatcattcc aaaataaaac tgactctgtt tctctacagt
     9361 attttacaca tatacattgt cctaaatttc taggacaaag tctacaaaga caattctttt
     9421 ctgttccatc acatctatca ttcttaaaat ctatacaata cttacaattt atacttgttc
     9481 caacagccat aacaaacctc ctgttcctct cttacataca gttaaaagta tatcactttt
     9541 aatcttttct ttaaaacaaa aagattctta taaatactat atttatttaa attgtaagtt
     9601 taaaagaaac actactatat ttgtttaatt ctcatttatc ttataaacta ttattaaaaa
     9661 ttttcttcct tatcaattgt cttattatct aaaaaccctg tagttttatc caatataaca
     9721 aaatcaccta tacaaggttg aatctcattg catactttaa tatcatcttc taacaaatcc
     9781 ttatttaata tctttctttt atataaaaca tctaaaaaga taaataataa aaccatagaa
     9841 attaaagatg caatgttgac cataaatgtt ccattaacat aacttacact tggaataaac
     9901 attataatta aatagcctac ggcatgtaca aatacaaatc caattagtat actcatagaa
     9961 atataaataa aatatctagg tatttcaaag aaatatggtc tatataaata tttaaaattc
    10021 attattttta aaattaatct tgaaagataa ataagtaata ctgaaattaa cactaagatt
    10081 attataaata taaactgtct ccctaaataa aaagaattgg tatcaaaatt taaatttaat
    10141 aaattaaaaa ttaataagac tttctcacca ataaaaccta aagaatttct aacccaaata
    10201 agtttatcac taattaaaag ctcacttact gaaaagatta aaattaaacc ctccataaag
    10261 aatattggaa ataccccagc aaacattcta tccttaacaa ttaaattcat aataaaaatt
    10321 attgaagtaa ataataatcc taatgctatg aaataaagaa aacttataat aagcttatct
    10381 aaactaaaag aattcctata tggtatataa aaaattaatt ttataaatat gttgattagc
    10441 ataggtatta gagaaactcc ataaattaaa ataactttag aaaagaaata tttttttgta
    10501 ttctcctctc ccacagctaa attttccttt agctcctttt ctttatcctg aaataaaaat
    10561 ccaacactcc aaactaaaaa taaaaataat aaaaatttat ctacactacc cattgaaaaa
    10621 catttattta ttatggaaat atctaaatcc ccttgccctg cctgaaaaaa gtatcttatc
    10681 atactgtaat ttcccattaa aaagaaataa gctatcccta aaactattaa tcctctaatc
    10741 cctctagact tgtatttatc tagaattaag ttattactct tcataaaatt ctccattcat
    10801 taaagtataa attctctata aatatacttt tataaatata tttttatgat ttttatttct
    10861 ttaagtagca aattttattt aaaaacatat attaatataa agagtgatta attgcttctt
    10921 cattttttga ctactcctct ccttagaaaa ggaagaagtg cttaatacat tccttgctta
    10981 ctttgcaagt ttcctcccat ctaaaaataa aaaaaacagg tgatttcacc tgttttttta
    11041 tttatataaa ttcttatatt tatctaacaa aaacttggaa taatatcatt aaaactactc
    11101 caggtattcc taaaatacct gctattaaag cagttaacaa atttattcct atagttattc
    11161 caaaataagc tccaaagtaa ttaacaatca taagtagtat agccccaaag acactattaa
    11221 taaccaaacc tattattttc cctattgatc caaatattaa tcttaaaatt aatattgcaa
    11281 caagaactcc tacaattaga agaattatat catctaagga taaagctcct atatccataa
    11341 atctaccacc tttttaaatt atacttatcc tagcattctt aaatatatgt tccatactaa
    11401 cttttatacc tcttttttta gcttctctta ttaggtattc atatctagcg ttaatatctt
    11461 gctctttata tattaaagat tctataagtt tatcatcaca agcattttga aaagctgatc
    11521 ttgcaacctc catttctagt atggtttgct caatagcttt taatatttcc tcatcttcat
    11581 ttgaataatc tatctttttt attaaataat cagaaatttt ctttttatcc ataactaaca
    11641 cctccaactt attcatttgg aattattacc aaaatgtcca agttatattc aggtgttata
    11701 aagatttaac tttgtatttt aactttttta actagttaat tcccttgtat ttatattatt
    11761 taattaccga tataaagatt taatactata ttttaatctc aatttgtatt ataaaataat
    11821 ttatatctta tttagaatca caagccctaa ataatcttta tataacagtt ctaccttcta
    11881 atgctttaga taaagttact tcatctgcat actctaaatc tcccccaact gggattcctg
    11941 ctgcaattct agtaacctta acatctaatg gctttaatat ctttgaaata tacatagctg
    12001 tagcttcccc ttcaatagta gggtttgtag ctacaataac ctcttttaca tctccgctca
    12061 ttcttgctac taattctctt attcttatat cctgtggtcc tcttccttgc attggagaaa
    12121 tagttccatg taaaacatga tataaaccat taaattcttt aactttttcc attgtcataa
    12181 tatccttagg ttgttcaacc acacatatta tatctttttc tctattagga tttgaacata
    12241 ttgcacatgg atctgtatct gtaaagttac cacatacaga acaatattta atagttcctc
    12301 ttgcttttac taaggcattt gcaaattcct ctacctcatc ctttggcaaa ttcaaaatat
    12361 gtaaagttaa tctttgagct gatttttttc ctatactagg taacttagca aactcttcaa
    12421 ttaacttttc tatagctact ggataaaatt ccattatttt tcctcctatt atttaaacta
    12481 aattctctat atttataaaa aactccgctg agaagtttca gcagagtttt caaaattaat
    12541 aagtatttct attagaataa tcctggcatt cccattccac cagttaattt acccattttt
    12601 tctgaagttt cttcagaagc tttttctaaa gcttgcttaa cagctgagaa aactagatct
    12661 tctaacattt caacatcatc tggatctaca acttctggct ttatattaag tgcgattaat
    12721 tctttctttc cgttaacttt tgcaacaact gcaccaccgc cagctgagcc ttcgaattct
    12781 gatgcttcga tttcctcttg cattgattgc atttgttttt gaagcatttg tgcttgcttc
    12841 attaaattat tcatgtttcc cattccgcct ggaaatccac ctcttgccat acttacatcc
    12901 tccttaaaaa ttaactcctt aatattatat agtatttttg aaaaatttac tataatatta
    12961 gattatatgc ttaatcatta ttatttggtt acaattttat tctattcttc aactatttct
    13021 agaccatcac ccataatacc tgaaagtcta gcttctaaat cagcttcctc agctttttcc
    13081 tcttctgttc cttgttcaac actatataca actcttacag gttcctttaa aacttctgaa
    13141 aatatatcat ttactatttt agagaaatca tgtttttcaa gtctttcttt attatgtcta
    13201 tattcataag tatatctaaa ttcaacaaca cctttattgc aagaaacttt gcttataccc
    13261 tttgctaagg ctatataaac catatacttt cctctactct taaaagtttc aagtatatct
    13321 ttccaaacct ttcttaaata ctctggtgtt agggttgaat tttcatttac cacttcataa
    13381 ttatttgaac tttttataac attctgttgt ttattattta aagttttatt atttgaagct
    13441 tcatttgaaa cttgtgattt aacctcttct aaattagtat taacctgtat atttccactt
    13501 cttattgctt gttctaactt atttattctt gctagcataa cttccttaga tgtatcatac
    13561 tctattttgc acatttttat tactgcaagc tctaaataaa ctctagcctg tttactaagt
    13621 ttagcctctt catcacattc ttgcacaatt cttatgcatc tcatgatttc ttcaaccttt
    13681 aacttagcac cttgcacttt aagtttttct atattttcct cagcagtatc taaaacttcc
    13741 tctggatttg aagttacctt tatcataagc aagtttctat aatgaactat taagtccttt
    13801 atgaaaagag tcatatcctt tcctgtaaga actaattctt ctattatttc catagcactt
    13861 tctatatctc tttttattat agaatccact attttaaata aattatcatt agttacaagt
    13921 cctaacatag aaactaattc atcataatct accttaccat cccctataga aatagcttga
    13981 tctaatatac ttaaggaatc tctcatagct ccatcagata ctcttgcaac aagatctaaa
    14041 cttttatcat cacagaatat cccttgctca gtaactattt ttcttaatct tcctgttata
    14101 tcttttctac taatcctttt gaagtcaaat ctttgacatc ttgataatat agttataggt
    14161 aatttttgag gatctgttgt agctaaaatg aagactacat tctttggtgg ctcttctaaa
    14221 gtctttaaaa aagcattaac tgccccaata gaaagcatgt gaacctcatc cattatgtaa
    14281 actttaaatc tagcttcttg aggtgggtat tgaacatcat caattatatc tcttatgtta
    14341 tctaccccat tgtttgaagc tgcatcaagt tcagttatat ctattgctaa tccttcattt
    14401 atttttttac acatctcaca ctgattacaa ggctctccgt cttttaaatc taaacagttt
    14461 aaagccttag ccattacctt agctgtagaa gttttaccag ttcctctagt accacaaaat
    14521 aaatatgcat gagctgttct ttcatttatt atctgatttt ttaatgtagt ggttatatgt
    14581 tcttgaccta caacatcatt aaaggtttgt ggtctccact ctctatacaa agctttatat
    14641 gccaaattat tcacctctct aagcctgtat taacatataa ttattataca tcaaaacaag
    14701 ccataatacc tatcacactt attttatatt tttcttagtt aaaccattat atcatgatta
    14761 atattttctt ttttaaaaat aaatatataa ttatcttaat aatattttta tgctaacctt
    14821 gaaaggaatt tttatatgtt ttctataatt gttgccaaaa gtattaataa tatcattgga
    14881 gttaataaca aaatgccttg gaatattcca tctgatttaa aaaggtttaa agaactcact
    14941 atggggaaaa aaatattaat gggaagaaaa acctttgaat ctcttccttt tgttttacct
    15001 ggaagaactc atttagtttt aactacaaag aaagattttt cttataatca taaagatgta
    15061 attatttata atgatttaga taagcttatt tctgaatata aagatagcat ggaagaaata
    15121 tttgtaatag gtggtgggaa aatatattct gagtttttaa aatatgcttc aaaattatat
    15181 ataactgaag ttttaaagga atataaagga gatacttatt tccctaaagt agattattct
    15241 aaatggataa aaacttacga aagttctatt tatgaagaga atgctactaa atttaaatat
    15301 gtaatatatg aaattttata aagaataata aaaccgaata tattttaatt gaaaaacatc
    15361 aataaaatat attcggtaaa taatattaaa agcaaattat attcttgcgc tcaaaactcc
    15421 attttccata gtgtatacac aatcacatat ttcatctata tcgtcactgt tatgacttgc
    15481 taataagatt gtaacagagt tagactttag ctttaataaa atttctctaa tttgttttac
    15541 accttctgaa tcaagaccat tcataggttc atcaagtatc aatagtttag gattctccat
    15601 aagagcttgg gcaataccta atctttgttt catacctaaa gaaaatttct ttactatttt
    15661 tttatcttca ggatctaacc caactaaagc gattgtttct tttattcttt catcacttat
    15721 tttattgttt atactagcta atatttttaa atttttaaaa gcagaatatt gaagtaaaaa
    15781 tccaggattt tcaataatca ttcctacatt atctggaaaa cttccatttt ttataacctt
    15841 atcgaacaca gttatactac cttctgttgt attaattaaa ccgcaaatag ctttaaataa
    15901 catacttttt cctgatccat tcctaccaac tattccatat gccttaccac tctcaagttg
    15961 taaattaact ttattaagta cttttgtacc ttttatatct tttgttaagt tagaaatttc
    16021 tacaacattt ctatttccca tgtatttttt cctcctaccc ctatatgttt aatacgtaat
    16081 atctaataaa tctctcttta ctattaatct attaataata actaaattaa taaatataag
    16141 tattcctaaa aatataaatg accaattata actaaaatta taaattatat gttttgataa
    16201 tatcccttga tttaatggaa taaacttatc tatattaaaa atatttccta tagaaattga
    16261 tacataaaat tctactatta aaattaaaaa tgatttaatg tggcttttag taaccattat
    16321 attaaaaata tacacagtta tagcgtagta actggataat gataataata caacaatttg
    16381 taaagtatca attatattgc taaaattaaa acctttattt aatatagctg taaatataaa
    16441 ttcaataaaa gttcctaaag taaaatatat aatacaagct aaaaagcatg taatttgtaa
    16501 acagatattc caatactgtt tatttcctaa tcgtggtata acatagatac ttcttgtact
    16561 aagttcatta aatatataat ttccaataat atatattaaa aaagcttgat aaaatgtcca
    16621 tgtaaatatt tcagatatat tatctaaatt aattggtcca gcaaatataa cttcaagaat
    16681 ttcattaaaa ctaaaattgg aatcataatt attaattttt actgaaaaaa cataaataaa
    16741 aataacaatt
//
LOCUS       DAEVGI010000042        16695 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEVGI010000042 DAEVGI010000000
VERSION     DAEVGI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 16695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16695
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.042"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            112..1452
                     /locus_tag="K8M77_001195"
     CDS             112..1452
                     /locus_tag="K8M77_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBI7085702.1"
                     /translation="MYIKLTKKIKFSAVQILALGFSMVILLGAIILSLPISSKSGEFT
                     PFLDSLFTSTSAVCVTGLITLNTAAHWNYFGKTLIIILIQIGGLGFMSFTTLFALMVK
                     KKITLRERLLIQEAMNTFGIQGLVRMIRYILTFTFSVEMLGALLLSTQFIPKFGLLKG
                     IYYSIWHSISAFCNAGFDLFGDNLVGYANNKTVILTIAALIVIGGLGFTVWSELYNYK
                     GYKKLSLHSKVVIMITAILLIGGAILMFIFEYNNPNTLKYMSFGDKLTNAFFASATTR
                     TAGFNSVSTADMTLAGKFLTILLMFVGGSPGSTAGGIKTATFGIVVLTLISIIRGRED
                     TEVFKKRISKELVYKAFAVLFIGFGIVVLGTLILAVTEPGFSLEYILYEVTSAFATVG
                     LTLGLTPYLSAIGKIIIIITMYLGRVGPMTVVLALANRKKPSAYKYPEDKILIG"
     gene            1475..2140
                     /locus_tag="K8M77_001196"
     CDS             1475..2140
                     /locus_tag="K8M77_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBI7085703.1"
                     /translation="MSSKQFVIIGLGRFGSSVAKTLYALGHDVLAIDYNEDLVQEISD
                     SVTHAVQMDATDENALRTLGLRNFDVAVVTIGANIQASIMATLLVKDMGIKYIIAKGN
                     SDLHAKVLYKIGADRVILPEKDMGVRVAHNLVSSSILDYIELSPDYSIIEIESPKEWY
                     GKSMKELSLRSKYGINVMAIKRNNEVNISPDADDVINKDDIVVAIGSAEDLTKLEGKI
                     FRH"
     gene            2166..2939
                     /locus_tag="K8M77_001197"
     CDS             2166..2939
                     /locus_tag="K8M77_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBI7085704.1"
                     /translation="MLEIESKNNNLFKEIKRLKEKKYRIKSNKYLIEGLRFVEEAIKS
                     KVSIDSIIFTDSFKEKNPDLFLKINENIKLIQMNEALLKQLCSTENPQGIVGVINMQN
                     KELKSGELVVLVDKVQDPGNMGTIIRTAHAAGAAGIVMTKGTVDIYNDKTLRSTMGSI
                     FYIPIVEDDSLDFVKSLKKEGYKLVVSSLQGKNNFFEENLQGKVMIAVGNEGNGVSDE
                     VYDIADIKVKIPMPGEAESLNVAVATSIMIYEKIRQSFK"
     misc_binding    3155..3380
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3550..4569
                     /gene="pheS"
                     /locus_tag="K8M77_001198"
     CDS             3550..4569
                     /gene="pheS"
                     /locus_tag="K8M77_001198"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBI7085705.1"
                     /translation="MQDKLNQIKELALVEIKKAKDSTTIDTIRVKYLGKKGELTTILR
                     GMGSLSKEERPIVGKLANEVREVLEAELEAVTKAVKEAEKQEKLKNEVIDISMPGKKQ
                     TIGKKHPLEQTLDEMKKIFVSMGFAIEDGPEVEKDYYNFEALNIPKNHPARSEQDTFY
                     INDNIVLRTQTSPVQARVMEKQQPPIKMISPGKVFRSDAVDATHSPIFYQMEGLVIDK
                     DITFADLKGTLELFAKKMFGDKVKTKFRPHHFPFTEPSAEMDATCFVCNGKGCKVCKG
                     EGWIEILGCGMVHPQVLRNCGIDPEVYSGFAFGFGVDRMVMLKYGIDDIRLLYESDMR
                     FLNQF"
     gene            4593..6974
                     /locus_tag="K8M77_001199"
     CDS             4593..6974
                     /locus_tag="K8M77_001199"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBI7085706.1"
                     /translation="MKVPFNWLKDYVDIDMTAEELGDALTLSGSKVEEVVTAGAEIEN
                     IVTCQIDKIEQHPDAEKLKICQVNIGSEMIQIVTAADNMKEHDKVPVALHGAILADGS
                     KIKKGKLRGEVSNGMFCSEEELGIADGKEVIGLMILPEDAPIGVCIKEYLGLNKQVLD
                     FEITSNRPDCLSVLGMARETAATTRKTYRMPNTKFEVKCDKNINDEIKVSVNDKLCNR
                     YMARGVKNVKVAPSPTWMQERLLDAGVRPINNIVDITNFVMLELGQPMHAFDRRELKS
                     SNIVVERAKDGDKFVTLDEIERELNSYVLCIKDGEEIVALAGIMGGLNSGVKDDTTDI
                     VFESANFDGTNIRVNSKNLGLRTESSSRFEKDIDPNLAALALDRACALVEELGCGEIM
                     EGTIDVYNNVKEEKTLEVSVSWINKFLGTSLEAKEMKRCLDSLDLKTTINGDTLEILV
                     PTFRIDVDIREDVAEEVARIYGYNNIPATLISTSTEKEAKDKKQLLDEKVIDTMLASG
                     LNQSISYSFVSPKVFDKICVPEDSILRNVVKIKNPLGEDYSVMRTSTLPSMMESLARN
                     YSRSNEIVRLFEIGKTYIPNSDENELPTERNILTIGIYGQCDYFDLKGIVENLLDSLG
                     LKSASFKRMSENPSYHPGKTSELILGRTSLGVLGEIHPDVNENYGVDVPCYVAELDLD
                     AMYNFSKTEKAYKPLPKYPAITRDIALLVDDAVLVQEIEDAIRKAGGQVVEKVELFDV
                     YKGAQIPEGKKSIAYAIAYRDPSKTLKDKDINKVHDKILRALEYKLGAQLREQ"
     gene            7055..8410
                     /gene="zapA"
                     /locus_tag="K8M77_001200"
     CDS             7055..8410
                     /gene="zapA"
                     /locus_tag="K8M77_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBI7085707.1"
                     /translation="MATVTVTINGVEYNLKGHEDGEYLKKVAEYVEEKTQEMATKNNK
                     LSALGVLSLSALNIADELFKGNDEYNQLIDYYEKVKSELEKSKKEIEDLKELEGESVS
                     LKEKLDKITSEKEALEKNLNELKDKKEEIEKSREELNNKFNKLNSENSNLKEELKNTK
                     NRMNNSNQEIANLKKEIERLKSENNSLKSAKDKNSHEVEKLSKELKEVKSNNAELNKT
                     IEISRNKEKNLSNEINNLKSKNNNVEKELRDLKEKNNSLSSIVNEAKKNLELLNKEIN
                     SLKERNKTQREENEKLTLEGENLKINCKEIEEKLEGLNKENGQLKETSELLNKEKIWI
                     KDQNSGLKKQILELEENLQLALEEKDALGKKISEDMEVEMKALKEEAKEVKAEMEILE
                     EEAKKLKREKELLMENNKELRRNWQTAKYKLLDLEQKYLDSQVKLATSKKSNNVLLKK
                     K"
     gene            complement(8496..9284)
                     /locus_tag="K8M77_001201"
     CDS             complement(8496..9284)
                     /locus_tag="K8M77_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBI7085708.1"
                     /translation="MLYDCHIHTEFSADSELKIKDILKKAEKNNLGIITTEHLDYNLK
                     THPQFAELDIEGYLKEYSKYRGENMQQGVEIGLAMNNVDENFAFYEKYKDKFDMIIGS
                     IHSINGVTLSRFLKQDTRPKEVVYGEYLDNMLKCIEFYDIFDTLGHIDYPCRYSIYDD
                     KEMTLKDFKPKFQAIFKTLIAKNKVLELNTTRLDDETSFKNMVEIYSYYRELGGKYIT
                     LGSDSHKIEHIGRNFEKLYKFLEKTKLTPCYFKDRKLIPCSYPL"
     gene            9485..11836
                     /locus_tag="K8M77_001202"
     CDS             9485..11836
                     /locus_tag="K8M77_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI7085709.1"
                     /translation="MERIELLAPAGSMESLIAAINRGADAIYLGGSKFSARAYASNFD
                     NETMEKAVDYAHTYGVKVYVTVNTLIKENEFQEAVDYIGVLYKIGVDALIIQDVGLAK
                     RIREVYPDFELHASTQMSIHNGEGALFFRENGFFRIVLSRELTLDEIKYISKDLGIET
                     EIFVHGALCVCYSGQCLMSSLIGGRSGNRGRCAQSCRLPYTLIREKDKKETKGFLLSP
                     KDICTIENVEDLIKTGTSSLKVEGRMKRPEYVAGVIESYREAIDNSYRNIKKSQERNK
                     LKLKKLFNREGFSTAYMYKNVGKDMMAFKTPRNTGVLLGEVLKNGEVLLLDDLSLKDG
                     IRTNDDGFTVSKILMGNREVTEAKKGDKVKIFPKKYKANDKLYKTSDTKLLNELKTSY
                     ENPYERKINLKAYMKFKVNEPMELTVEYNGNYFTKTGDMVQVAVNRPMDKEKVEKNLK
                     KSGDIPYEISEIEYVTFEDGFAAVSSINNLRREVLEEIRNSEIKKYKRVIEKQNNIEL
                     NEKNGEMPEVLVVVNTNEQLRASIDCGIKNIALDIFGKNQGQLNKNAIKEFKDKGINI
                     YIKAPTIIKEEFEIISNIIEENLNLIKGLVTANTGIINRFKDKTNIIGDYKLNIFNSC
                     GMKFYNEHMMGSCLSLELNKKELNKMLKKYSKGAQIFVYGRPEVMVSEYCPIGSTFGG
                     KCTSKNCDNQCVSSTFTLRDRMNQDFVIRTDIFCRSHIYNTVPVNLIQEIDEIKSLGV
                     NSFRLDFVDENYEEVRQVIEALNKEEALRLKDYTKGHFKRGVE"
     gene            11850..14210
                     /locus_tag="K8M77_001203"
     CDS             11850..14210
                     /locus_tag="K8M77_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBI7085710.1"
                     /translation="MNDRVLRVLEFNKIKELVKGYAITKSAKEMVLDLKPYDSVYDVK
                     EHLEETKEALDILMRKGNPPFEGLYDVKEAITRAEKGGVLSIEGLLRIGNMLSVTRKL
                     SDFLARKEEEEEHRILEGMREGLIVLRGVESAISKAIVSEDEIADSASDKLYSIRRSL
                     KEKNSSIRDKVNSIVRSNAQYLQDSLYTVRGDRYVIPVKAEYKSQVPGLVHDQSSTGA
                     TLFIEPTALVNLNNEIKELMLKERAEIERILAELSALVYKNIDVIKVNFNIIVELDFI
                     FAKAKYGSDLGGTMPIVNEEGVIDLMDARHPLIPKDKVVSSDIYLGREFSTLLITGPN
                     TGGKTVTLKTTGLIELMGLSGLLIPASENSSISFFEEIFADIGDEQSIEQSLSTFSSH
                     MTNIVKIMEKANNKSFVLFDELGAGTDPTEGAALAISILENLRARGCRIMSTTHYSEL
                     KGYALKTENVENASVEFNVETLRPTYRLLIGVPGKSNAFEISRRLGLKDNIIEEAKKV
                     ISTESLQFEDLIQSLQEKSIKAENDAREAAILRNDAEKYKNRYKEKFERIESVRDNVY
                     ADARREAKQILDSAKEEADTILKNMRDLERMGISSDARRKLEAERGKLRDKISDAEAR
                     LQKKKEEQKGEELKKIEVGMEALLPSINQKVIVLSKPDNKGEVQVQAGIMKINVKAKD
                     LRVAKETKEEKKIKKREARLNLRQVDPSIDLRGMDSEEACYTADKYLDDAYVAGRGEV
                     TLVHGKGTGVLRKAINDMLKKHPHVKSHRLGEYGEGGTGVTVVILK"
     gene            14474..16657
                     /locus_tag="K8M77_001204"
     CDS             14474..16657
                     /locus_tag="K8M77_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBI7085711.1"
                     /translation="MKAKKKNKKIKLNKGRVLAFLLAIAILFLALSGRLLYISIFRSK
                     DYKAMAEEQWTNEIQIDARRGRILDRNNRELAVTANVFRVDLDLITLRKYIDKKDTDN
                     IEIAKLLAEALEMDEEKVLSRIELTYPSGNPANSATLIRRIEKEKADKVKELDINGVI
                     VSEDTKRYYPNGDFLSHTLGTTNADGEGLSGLELYYNEELMGIPGVRVSEVSGNSTSN
                     PYSETSFTPPVDGKDMTLTIDENIQYFVEKVAEDALNKHNADSVSIAVMNPNNGEILG
                     MVNKPGFNPNNPYEGSEAFKGKDESAKLQNMWRNTIVSDAFEPGSIFKIITSIAAIEE
                     NIAGKDEVYYCDGSLNVAGKNIKCWKPGGHGVQNFNQTLENSCNVAFMEMGAKLGAEK
                     LNEYIKLFGFGTQSGIDLPGEATGIVKNVEDISSVDLATISFGQTNTMNGIQFMTALN
                     AVANGGDLIQPHIMKELSHKDDNGTKIIDEVFVPKIQENIVDEKSTMRVKEALESTVS
                     NGSSKDAGIEGIKVAGKTGTAEKVDSETGTYGAGYIASFAGFAPYDNPQVSLIVIIDN
                     PKNGEHFGGIVAAPFAGELFNNIFNYISLNEGQLDVKDESVIITDLRGDKVTSAEKTL
                     NELGINCVIEGDGTFVTSVKPIPGYKVKKGDSITLYAGSRFDRNEKEIVVPDFRKLNK
                     EEAEEILKSLGLKGEFEGEGEIKEQSISAGEIIKSGYKIKFKLEG"
BASE COUNT         6767 a         1737 c         3125 g         5066 t
ORIGIN      
        1 aaattacttt taaattataa aaataagata actttacatg tacaaattaa aaataagata
       61 taataaaatt aaaggaaaaa tataatatta ttttaagttg ggggtttaat tatgtacatt
      121 aaactgacaa aaaagataaa atttagcgct gttcagatac tagctttagg tttttctatg
      181 gttattttat taggtgctat aatattaagt cttccaatat catctaaaag tggtgaattt
      241 actccttttt tagatagttt atttacttca acttcagctg tatgtgttac gggacttata
      301 acattaaata cagcggcaca ttggaactat tttggaaaaa ctcttataat aattttaata
      361 caaataggtg gattaggctt catgtccttt acaactctat ttgctttaat ggttaagaaa
      421 aaaataacct taagggaaag acttcttata caagaagcta tgaatacctt tggaatacaa
      481 ggattagtta gaatgattag gtacatatta acatttacat tttcagtaga aatgttaggt
      541 gcattattat tgtccactca atttataccg aaatttggat tattaaaagg aatatactat
      601 agtatttggc attctatatc agctttttgt aatgcagggt ttgatttatt tggagataat
      661 ttagttggat atgctaacaa taaaaccgta atattaacaa ttgcagcatt aatagtaatt
      721 ggtggtttag gatttactgt ttggtcagag ttatataatt ataagggtta taagaaatta
      781 tctttacatt ctaaagttgt aataatgata acagctatcc ttttaattgg gggagctatc
      841 cttatgttca tatttgaata taataatcct aatactctta aatatatgag ttttggagat
      901 aaattaacta atgcattctt tgcttcagca accacaagga ctgctggatt taactctgtt
      961 tctactgcag atatgacctt agctggaaaa ttcttaacta tattattaat gtttgtaggt
     1021 ggatcaccag ggtcaacagc agggggaatt aaaactgcta cctttggtat agtagtactt
     1081 actctaatct ctataataag aggaagagaa gatactgaag tatttaagaa gagaatttca
     1141 aaagaattag tgtataaggc ttttgctgtt ttatttatag gttttggtat agtggtactt
     1201 ggaacattaa ttttagcagt aacggaacct ggattttcat tagaatatat tctatatgaa
     1261 gttacatcag cttttgctac agttggatta actcttggat taactccata tctttcagcc
     1321 ataggaaaaa taataataat aataactatg tatttaggaa gagtgggtcc tatgactgtt
     1381 gttctagctt tagctaatag aaagaaacca agtgcataca aatacccaga agataaaata
     1441 ttaataggat aagtaagtta ggagtgtaag gcttatgtca agtaaacaat tcgttattat
     1501 aggattagga agatttggat catcagtagc taaaacttta tatgctttag gtcatgatgt
     1561 tttagcaatt gattataatg aggatttagt tcaagaaata tcagatagtg taactcatgc
     1621 agttcaaatg gatgccacag atgaaaatgc tttaagaact ttaggattaa gaaattttga
     1681 tgtggctgta gttacaatag gagctaacat acaagcaagt attatggcaa cattgcttgt
     1741 aaaagatatg ggaataaaat atataatagc taagggaaat agtgatttac atgctaaagt
     1801 tttatataaa ataggagcag acagagtaat cttacctgag aaggatatgg gagttagagt
     1861 agctcataac ttagtttctt caagcatctt agattatata gaattatcac cagattattc
     1921 tataattgag atagaaagtc caaaagaatg gtatggaaag tctatgaaag aattaagttt
     1981 aagaagtaaa tacggaataa acgtaatggc tattaagaga aacaatgaag ttaatatatc
     2041 tccagatgca gatgatgtta ttaataaaga tgacatagta gttgctatag gttctgcaga
     2101 ggatttaaca aaattagaag gaaagatatt tagacattaa tatcttctag aaaggattat
     2161 ttgctttgtt agaaatcgaa agtaaaaata ataacttatt taaagagata aaaaggttaa
     2221 aagaaaaaaa gtatagaata aagagtaata aatatcttat tgaaggttta agatttgtag
     2281 aggaagctat aaaaagtaag gtatcaatag attcaataat ttttacagac agttttaagg
     2341 agaaaaatcc tgacttattc ttaaaaatta atgaaaatat aaaacttata caaatgaatg
     2401 aagctctttt aaaacaactt tgttcaacag aaaatccaca aggaattgtt ggagttataa
     2461 atatgcaaaa caaggaatta aaaagtggag agctagttgt tttagtagat aaagttcaag
     2521 atccaggaaa tatgggaaca ataataagaa cagctcatgc agcaggtgca gcaggaatag
     2581 taatgactaa aggtactgtt gacatatata acgataagac tttaagatca acaatgggtt
     2641 caatatttta tattccaatt gttgaggatg attctttaga ttttgtaaaa tcattaaaga
     2701 aagaagggta taaacttgtg gtaagttctc ttcagggaaa aaataatttc tttgaagaaa
     2761 atcttcaagg aaaagttatg atagctgttg gtaatgaggg aaatggtgtc agtgatgaag
     2821 tatatgatat tgcagacata aaagttaaga ttcctatgcc tggtgaagct gaatctttaa
     2881 atgtagctgt agctacttct ataatgatat atgaaaagat aagacaatca tttaaataaa
     2941 taaagaggct ttataaaaga gacttttaat aaaacttaca aatatattct aagaattact
     3001 agagattctt ttaatactat ttgtaagttt tattattaac ttttcatata atctcttgat
     3061 ttttttataa gtaaaattct attaaatagg aaaaaataat aaatgccttg attataaata
     3121 ctattatatg tataatatac tttgaattga atagataaaa acattgatag agagagtaga
     3181 tatagcaatt tcttatcagg gaaaaagtgc catggactga aagcattttt agaaacctat
     3241 attgaagttc actctggagt tctaactgtg aaatataaag tagtagttag cggcatagtc
     3301 gttatttgaa attaagtgag tactaatttt tttagtacta attagggtgg taccacggat
     3361 atgaataccc cgtcccttgt ttttgaggga tggggtttat ttattttaat agaaatttgt
     3421 caataatgaa aatataccac taaaattaaa tctaagatta aataatgact aaaaaataga
     3481 tattagggtt ctaagagctt taagataaaa tctaatatat taattatata tcaggaaagg
     3541 agaatgaaaa tgcaagataa gttaaatcaa attaaagaat tagctttagt agaaattaaa
     3601 aaagctaaag atagtactac tattgataca ataagagtta aatatcttgg taaaaaggga
     3661 gaacttacaa ctatattaag aggaatggga tctctatcta aagaggagag accaatagtt
     3721 ggtaagttag ctaatgaggt aagagaggtt ttagaagctg aattagaggc tgtaacaaag
     3781 gctgttaaag aagctgaaaa acaagaaaag cttaaaaatg aagtaataga tatttcaatg
     3841 cctggtaaaa aacaaacaat aggaaagaaa catccattag agcaaacttt agatgaaatg
     3901 aaaaaaatat ttgtttcaat gggatttgct atagaagatg gtccagaggt tgagaaagat
     3961 tactataact ttgaagcctt aaacattcct aagaatcatc cagctagaag tgagcaagat
     4021 acattctaca taaatgataa tatagtttta agaactcaaa cttctccagt tcaagctaga
     4081 gtaatggaaa aacaacaacc accaataaaa atgatatcac ctggtaaggt atttagatca
     4141 gatgctgttg atgctacgca ttcaccaata ttctaccaaa tggaaggtct tgttatagat
     4201 aaagatataa cttttgcaga tcttaaggga actttagaat tatttgctaa gaaaatgttt
     4261 ggtgataaag taaaaactaa gtttagacca catcatttcc cattcactga gccatcagct
     4321 gaaatggatg ctacatgctt tgtatgtaac ggaaaaggat gtaaagtatg taagggagaa
     4381 ggttggatag aaatactagg ttgtggtatg gttcaccctc aagttttaag aaactgtgga
     4441 atagacccag aagtttatag tggatttgct ttcggctttg gtgtagacag aatggttatg
     4501 cttaagtatg gaatagatga cataagatta ttatacgaaa gtgatatgag attcttaaat
     4561 caattctagt tttaaaatag gaggtaaaaa agatgaaagt accatttaat tggcttaaag
     4621 attacgttga tatagatatg acagcagaag agttaggcga tgctttaact ttatcaggat
     4681 caaaagtaga agaggtcgta actgctggtg cagaaataga aaatatagtt acttgtcaaa
     4741 tagataaaat agaacaacat cctgatgctg aaaagcttaa aatttgtcaa gtaaacatag
     4801 gatcagaaat gattcaaata gtaacagctg cagataatat gaaagaacat gataaagtcc
     4861 cagtagcttt acatggtgca atattagcag atggaagcaa aatcaaaaaa ggtaaattaa
     4921 gaggagaagt ttcaaacgga atgttctgtt cagaagaaga attaggaatt gctgacggaa
     4981 aagaagttat tggacttatg attttaccag aggatgctcc aattggtgtt tgtataaaag
     5041 aatatttagg tcttaataag caagttttag attttgaaat aacttcaaat agacctgatt
     5101 gtttaagtgt tttaggtatg gctagagaga cagcagccac aacaagaaaa acttacagaa
     5161 tgccaaatac taaatttgaa gttaaatgtg ataaaaacat aaatgatgaa ataaaagttt
     5221 cagttaatga taagctttgt aatagatata tggctagagg agttaaaaat gtaaaggtag
     5281 ctccatcacc aacttggatg caagaaagat tattagatgc tggggttaga cctataaata
     5341 acatagttga tattactaac tttgttatgt tagagcttgg tcaaccaatg catgcctttg
     5401 atagaagaga attaaaatct tctaacatag tagttgagag agctaaagat ggagataaat
     5461 tcgtaacttt agatgaaata gaaagagaat taaattcata tgttctttgc ataaaagatg
     5521 gagaagaaat agttgcttta gctggtataa tgggtggatt aaactcaggt gttaaagatg
     5581 atacaacaga tattgtattt gaatcagcta actttgatgg aactaacata agagttaatt
     5641 ctaaaaattt aggattaaga actgaaagtt catcaagatt tgaaaaagac atagatccaa
     5701 acttagcagc tttagcttta gacagagcat gtgctttagt tgaagaacta ggctgtggag
     5761 aaattatgga aggcactata gatgtctaca ataatgttaa agaagaaaaa actctagaag
     5821 taagtgttag ctggataaat aaattcttag gaacttcatt agaagcaaaa gaaatgaaga
     5881 gatgtttaga tagcttagat ttaaaaacta ctataaatgg agatacatta gaaatattag
     5941 tacctacatt tagaatagac gttgatataa gagaagatgt agcagaagaa gttgcaagaa
     6001 tctatgggta caataatatt ccagctactt taatatcaac tagtacagaa aaagaagcta
     6061 aggacaagaa acaattatta gatgaaaaag ttatagatac aatgttagca tcaggcttaa
     6121 atcaatctat aagttactca tttgtaagtc caaaggtatt tgataaaata tgtgtaccag
     6181 aagatagcat acttagaaat gttgttaaaa taaagaatcc actaggagaa gattatagtg
     6241 ttatgagaac ttcaacactt ccttcaatga tggaatcttt agctagaaac tattcaagaa
     6301 gtaatgaaat agttagatta tttgaaatag gtaaaactta tattccaaat tcagatgaaa
     6361 atgaattacc aactgaaaga aatatattaa caattggtat ttatggacaa tgtgattact
     6421 ttgatttaaa aggaatagtt gaaaacttat tagattcatt aggtcttaag agcgcatcat
     6481 tcaagagaat gagtgaaaac ccaagttatc atccaggaaa aacttctgag ttaatattag
     6541 gtagaacttc actaggtgtt ttaggagaaa ttcacccaga tgttaatgaa aactacggag
     6601 tagatgttcc ttgctatgtt gctgaattag atttagatgc tatgtataac ttctcaaaaa
     6661 cagagaaagc ttacaaacca ttacctaagt atccagcaat aacaagagat atagctttac
     6721 ttgttgatga tgcagtttta gttcaagaaa tagaagatgc aataagaaaa gctggtggtc
     6781 aagtagttga aaaagttgaa ttatttgacg tttataaagg tgctcaaata ccagaaggta
     6841 agaagagtat tgcatatgct atagcttata gagatccaag caaaacttta aaggataaag
     6901 atataaataa agttcatgat aagatactaa gagctttaga atataagcta ggagctcaat
     6961 taagagagca ataatagttt taaatatgtt atgtatatgt taaaatgtac ataacatatt
     7021 ttttattatg gaagtaagag gtgtttaagg aagtatggcg actgttacgg taacaataaa
     7081 tggagttgaa tataatttaa aaggccatga agacggagaa tatttaaaaa aagtagctga
     7141 atacgtagaa gaaaaaacac aagagatggc aacaaaaaat aataaattaa gtgcacttgg
     7201 agttttatct ttatctgcat taaacatagc tgatgaatta tttaaaggaa acgatgaata
     7261 taatcaactt atagattatt atgaaaaagt aaaatctgaa ttagaaaagt ccaaaaaaga
     7321 aatagaagat cttaaagaac tagaaggaga aagtgtttct ttaaaggaaa aactagataa
     7381 gattacttca gagaaagaag ccttagaaaa gaatttaaat gaattaaaag ataaaaaaga
     7441 agagattgag aaaagcagag aagaacttaa taataaattt aataagctaa atagtgaaaa
     7501 ttctaattta aaagaagagc ttaagaatac aaagaataga atgaataatt ctaatcaaga
     7561 aatagctaat ttaaagaaag aaattgaaag attaaaaagt gaaaataata gtttaaaatc
     7621 agctaaggat aaaaactcac atgaagttga aaaattaagt aaagaactta aagaggttaa
     7681 atcaaataat gctgagttaa acaaaactat agaaatctca agaaataaag agaaaaactt
     7741 aagtaatgag attaataatt taaaaagtaa aaacaataat gtggaaaaag agcttagaga
     7801 tcttaaggaa aaaaataata gcttaagtag tattgttaat gaagctaaga aaaacttaga
     7861 actattaaat aaagagatta atagcttaaa ggaaagaaat aagactcaaa gagaagaaaa
     7921 tgaaaaatta actttagaag gtgaaaatct aaagattaat tgcaaggaaa tagaagaaaa
     7981 acttgaaggt ttaaataaag aaaatggaca acttaaagaa acttcagagc ttttaaataa
     8041 agaaaaaatt tggattaaag atcaaaatag tggtttaaag aagcaaattt tagagttaga
     8101 agaaaatctt caattagctt tagaagaaaa agatgcttta ggtaagaaga tttcagagga
     8161 tatggaagtt gaaatgaaag ccttaaagga agaagctaaa gaagttaagg ctgaaatgga
     8221 gattttagaa gaggaagcta aaaagttaaa aagagaaaaa gaacttttaa tggagaataa
     8281 taaagagtta aggcgtaact ggcaaacagc taagtataaa cttttagatt tagagcaaaa
     8341 atacttagat tctcaagtta agcttgccac ttcaaaaaaa tcaaataatg ttcttttaaa
     8401 gaaaaaataa aagtgtattt agagactgta agagatttat gtgaaaataa attttcttac
     8461 agtcttttat ggtttaataa aaaataaaat tatttttata ggggatatga acatggaatt
     8521 aattttctgt ccttaaagta acaaggagtt aattttgtct tttctaagaa tttataaagt
     8581 ttttcgaaat ttctacctat atgttctatt ttgtgagaat ctgaaccaag ggttatatac
     8641 ttacctccaa gttctctata gtatgaatag atttctacca tgtttttaaa ggaagtttca
     8701 tcatctaaac gagtagtatt aagttcaaga actttatttt tagctattag tgttttaaaa
     8761 atagcttgga atttaggttt aaaatcctta agagtcattt ccttatcatc ataaatactg
     8821 tatctacaag gataatctat atgtcctaag gtatcaaaaa tatcataaaa ttctatgcat
     8881 ttaagcatat tatcaagata ttcaccataa acaacttctt taggtctagt atcttgtttt
     8941 agaaatctag aaagagtaac accatttata gaatgaatag aacctattat catatcaaat
     9001 ttatctttat atttttcata gaaggcaaag ttttcatcta cattgttcat tgcaagtcca
     9061 atttccacac cttgttgcat attttctcct ctatatttac tgtattcctt taaataacct
     9121 tcaatatcaa gctcagcaaa ttgaggatga gtttttaaat tataatctaa atgctctgta
     9181 gttattattc ctagattatt tttttcagcc ttctttaata tatccttaat ttttaattca
     9241 gagtcagctg aaaattctgt atgtatgtga caatcataaa gcattttgta acctccgcat
     9301 aaatattagt ataatttaat tttatcataa cttagaaaaa tattaagatt tttgttctat
     9361 aagtgcttaa ataaaaagac aaataatgaa atgtagaatt gtattgtgta attgtgtata
     9421 atacaaagta ttgaataaat taactaagga aagacctagt tgaattttag gagagatgaa
     9481 ttttatggaa agaatagagt tactagcacc ggcaggaagt atggaaagtt taattgctgc
     9541 cataaataga ggtgctgatg ctatatattt aggaggaagc aagttttcag caagagctta
     9601 tgcttctaac tttgataatg aaacaatgga aaaggctgtg gactatgctc atacctatgg
     9661 agttaaggtt tatgttacag taaatacttt aattaaggaa aatgagtttc aagaagcagt
     9721 agattacata ggcgttttat ataaaatagg agttgatgct ttaataatcc aagatgtagg
     9781 attagctaaa agaataagag aggtatatcc agattttgaa ttacacgctt caactcaaat
     9841 gagcatacat aatggagaag gagccctttt ctttagagaa aatggatttt ttagaattgt
     9901 tttatcaaga gaacttacct tagatgaaat taaatatatt tcaaaggact taggaataga
     9961 aactgagata tttgttcatg gagccttatg tgtttgctat tcaggacaat gtttaatgag
    10021 tagcttaata ggaggaagaa gtggtaatag aggaagatgt gctcaaagtt gtagacttcc
    10081 atatacacta attagagaaa aagataagaa agagactaaa ggattccttt taagtcctaa
    10141 ggatatttgt actattgaaa atgtagagga cttaataaaa acaggaacta gctcattaaa
    10201 ggttgaagga agaatgaaga gacctgagta tgttgctgga gttatagaat catataggga
    10261 agctatagat aattcttata gaaatattaa aaaatctcaa gagagaaata aacttaaact
    10321 taaaaagtta tttaatagag aaggtttttc tacagcatac atgtataaaa atgttgggaa
    10381 agatatgatg gcctttaaaa ctccaagaaa tacaggagtt ttacttggag aagttctaaa
    10441 aaatggagaa gtgcttttat tagatgatct ttcattaaaa gatggaataa gaactaatga
    10501 tgatggattt actgtaagta aaatacttat gggaaataga gaagttactg aagctaaaaa
    10561 gggggataag gtaaaaatat tccctaaaaa atataaagct aatgataagc tttataaaac
    10621 ttcagatact aagcttttaa atgaattaaa gacctcttat gaaaatccat atgagagaaa
    10681 aataaactta aaggcttata tgaaatttaa ggttaatgaa cctatggaac ttactgtgga
    10741 gtacaatgga aattacttta caaagactgg tgatatggtt caagttgcag taaatagacc
    10801 tatggataag gaaaaagttg aaaagaactt aaagaaatca ggagatattc cttatgaaat
    10861 aagtgaaata gaatacgtaa cttttgaaga tggttttgca gcggtttctt ctataaataa
    10921 tttaagaaga gaagttttag aagagataag aaattctgaa attaaaaaat acaaaagagt
    10981 tatagaaaaa caaaacaata tagaacttaa tgaaaagaat ggtgaaatgc cagaggtttt
    11041 agttgttgta aatactaatg aacaattaag agcttcaata gattgcggaa ttaaaaatat
    11101 tgctttagat atttttggaa aaaatcaagg gcaacttaat aagaatgcca taaaagaatt
    11161 caaagataaa ggaattaata tttatataaa agcacctaca ataataaaag aagagtttga
    11221 aataataagt aatataatag aagagaatct taacttaata aaaggtttag ttacagctaa
    11281 tactggaata ataaatagat ttaaggataa aacaaatatt ataggtgatt ataaacttaa
    11341 tatatttaat tcttgtggta tgaagtttta taatgaacat atgatgggaa gctgtttaag
    11401 tttagaatta aataaaaaag agttaaataa aatgcttaag aagtattcta agggagctca
    11461 aatctttgtt tatggaagac cagaggttat ggttagtgaa tactgtccaa taggaagtac
    11521 ctttggtgga aaatgtactt caaaaaattg tgataatcaa tgtgtaagta gtacatttac
    11581 tttaagagat agaatgaatc aagattttgt tataagaact gatatattct gtagatcaca
    11641 catatacaat acagtacctg ttaacttaat tcaggaaatt gatgaaataa aatctttagg
    11701 agtaaattct tttagattag actttgtaga tgaaaattat gaagaggtta gacaggttat
    11761 tgaagctcta aataaggaag aagctttaag attaaaggat tatactaaag gtcactttaa
    11821 aagaggcgtt gagtaaaggg gattaaagta tgaacgatag agttttaaga gttttagaat
    11881 ttaataaaat taaggaattg gttaagggat atgccattac aaaatcagct aaggagatgg
    11941 ttttagatct taaaccatat gattctgttt atgatgtaaa agaacattta gaagaaacaa
    12001 aagaagcttt agatatttta atgagaaaag gaaatcctcc ttttgaaggg ctttatgatg
    12061 ttaaagaagc tataacaaga gctgaaaaag gtggagtttt aagcatagaa ggcttactta
    12121 gaataggaaa catgttatct gtaacaagaa aactttctga ctttttagct agaaaagaag
    12181 aggaagaaga acatagaatc ctagaaggaa tgagagaagg gcttatagta cttagaggtg
    12241 tagaaagtgc tatatcaaag gccatagtaa gtgaagatga aattgcagat tctgcgagtg
    12301 ataaacttta tagtataaga agaagcttaa aagaaaagaa ttcatctata agagataaag
    12361 ttaattccat agtaagaagt aatgctcaat atcttcaaga ttccttatac actgttagag
    12421 gagatagata tgttattcct gtaaaagctg aatataaatc tcaagttcca ggacttgttc
    12481 atgatcaaag ttcaacaggg gctacacttt ttatagaacc aacggcttta gtaaacttaa
    12541 ataacgaaat aaaagagctt atgctaaagg aaagagctga gattgagaga atattagcag
    12601 aattatcagc cttagtatat aaaaatattg atgttataaa agttaacttt aatataatag
    12661 ttgaattaga ttttatattt gcaaaggcta aatacggtag tgatttaggt ggaacaatgc
    12721 ctatagtaaa tgaagaaggc gtaatagatt taatggatgc taggcatcca ctaattccta
    12781 aagataaagt tgtttcttct gatatttatt taggaagaga attttcaaca ttacttataa
    12841 cagggccaaa tactggtggt aaaactgtaa ccttaaagac tacagggctt attgaactta
    12901 tgggcttaag tggactttta ataccagcaa gtgaaaattc aagcataagc ttctttgaag
    12961 agatatttgc agatatagga gatgagcaaa gtatagagca aagcttatca actttttctt
    13021 ctcatatgac taatatagtt aaaataatgg agaaagcaaa taataaaagt tttgtacttt
    13081 ttgacgaact tggagctgga acagacccta cagagggagc tgcacttgcc atttcaatat
    13141 tagaaaactt aagagcaaga ggatgtagaa taatgtctac aactcactat agtgaattaa
    13201 agggatatgc attaaaaact gaaaatgttg agaatgcttc tgttgagttt aatgttgaaa
    13261 ccttaagacc aacttataga cttttaatag gagttccagg gaaatcaaat gcttttgaaa
    13321 tttcaagaag attaggtctt aaagataata tcatagaaga agctaaaaag gttatttcta
    13381 ctgaatcact tcaatttgaa gatttaatac aatcacttca agaaaagagt ataaaagcag
    13441 aaaatgatgc tagagaagct gctatattaa gaaatgatgc agaaaaatat aagaatagat
    13501 ataaagagaa atttgaaaga attgaaagcg taagagataa tgtttatgca gatgctagaa
    13561 gagaagcaaa gcaaatttta gattcagcta aggaagaggc tgatactatt cttaaaaata
    13621 tgagagacct agaaagaatg gggatttcta gtgatgcaag aagaaagctt gaagctgaaa
    13681 gaggaaagct tagagataaa ataagtgatg cagaagctag acttcaaaag aaaaaagaag
    13741 agcaaaaggg agaagaactt aaaaagattg aggttggaat ggaagcctta ttaccttcaa
    13801 taaaccaaaa agtcatagtt ctttctaagc cagataataa aggtgaagtt caagttcaag
    13861 ctggaattat gaaaataaat gttaaggcta aggatttaag agtagccaag gaaactaaag
    13921 aagaaaagaa aattaaaaag agagaagcaa gattaaactt aagacaggtg gatccatcaa
    13981 tagacttaag aggtatggat tcagaagaag cttgttacac tgcagataag tatttagatg
    14041 atgcttatgt tgcaggaaga ggagaagtaa ctttagttca tggaaaaggt actggtgttt
    14101 taagaaaagc tataaacgat atgctaaaaa aacatcctca tgtaaaatct cacagactag
    14161 gtgagtatgg agagggtgga acaggagtta ctgttgtaat attaaaataa atttttaata
    14221 ttaaagtatt acttaactta gattttgatt tatataaatt taagttaagt acaaaatcaa
    14281 taataattta aaaaataata ttaagagttc gtctagattt tttattagta gatttaaata
    14341 aactacgctt ataaagaatt ttagatgggc tcttttttag atacaattta ttaaaaaaag
    14401 catgagtata tttatgatat aatcaattta aactttgaga attatgtaaa attggtatag
    14461 aggaggctgt taggtgaaag caaaaaagaa aaataaaaaa attaaactta ataaaggtag
    14521 agtattagct tttttattag ccatagcaat attattttta gctttatcag gtagattgct
    14581 ttatataagt atatttagat caaaagatta taaggctatg gcagaagaac aatggacaaa
    14641 tgaaatacaa atagatgcta gaagagggcg tatcttagat agaaacaata gggagttagc
    14701 agttactgca aacgtattta gagtagattt agatttaata accttaagaa aatatattga
    14761 taagaaagat actgataata ttgaaattgc aaaattatta gctgaagctt tagagatgga
    14821 tgaagaaaag gttttatcaa gaatagaact tacatatcca tcaggaaatc cagcaaactc
    14881 tgctacctta ataagaagaa tagaaaagga aaaggctgat aaggttaaag agctagatat
    14941 aaatggagta atagtatcag aggatacaaa aagatattat cctaatggag attttctttc
    15001 tcatacttta ggaacaacta atgcagatgg agaaggatta agtggattag aactttatta
    15061 taatgaagaa cttatgggaa ttccaggagt aagagttagt gaagtctctg gaaatagtac
    15121 ttcaaatcct tattctgaaa ccagttttac tccacctgta gatggaaaag acatgacttt
    15181 gacaatagat gagaatatac aatactttgt tgaaaaggta gccgaagatg ccttaaataa
    15241 acataatgca gactcagtaa gcattgcagt tatgaaccct aataatggag aaattttagg
    15301 aatggtaaat aaacctggat ttaatcctaa taatccttat gaaggtagtg aggcatttaa
    15361 ggggaaagat gaatctgcaa aactacaaaa tatgtggaga aatacaattg tttcagatgc
    15421 ctttgagcca ggttcaatat ttaaaataat aacttctata gcagccatag aggaaaacat
    15481 agcaggcaaa gatgaggttt attattgtga tggtagttta aatgtagcag ggaaaaatat
    15541 aaaatgttgg aagcctggtg gacatggagt acaaaatttt aaccaaacat tagaaaattc
    15601 atgtaatgtt gcttttatgg aaatgggggc aaaattagga gctgaaaagt taaatgaata
    15661 tattaaactt tttggatttg gaactcaatc aggaatagat ttaccgggag aagctacagg
    15721 aatagttaaa aatgtagaag atataagttc tgtggattta gcaactatat cctttggaca
    15781 aactaataca atgaatggta ttcagtttat gacagctcta aatgctgttg caaatggagg
    15841 agatttaatt cagcctcata ttatgaaaga attaagtcat aaggatgata atgggacaaa
    15901 aattatagat gaagtttttg tgcctaaaat acaagaaaat atagtagatg aaaaaagtac
    15961 tatgagagtt aaagaggcct tagaatctac tgtttctaat ggatcttcaa aggatgcagg
    16021 aattgaagga ataaaagtag caggaaaaac tggtacagca gaaaaggtag attctgaaac
    16081 aggtacttat ggggctgggt atatagcatc ctttgctgga tttgcaccat atgataatcc
    16141 acaagtatct ttaatagtaa taatagataa tcctaaaaat ggagagcatt ttggaggaat
    16201 tgttgcagca ccttttgcag gagaattatt caataatata tttaactata tttcattaaa
    16261 tgaagggcaa ttagatgtaa aagatgaaag tgtaataatc actgatttaa gaggagataa
    16321 ggtaactagt gctgaaaaaa ccttaaatga attaggaatt aattgtgtaa ttgagggaga
    16381 tggaaccttt gttacttcag ttaaacctat accaggctat aaggttaaaa agggtgatag
    16441 tataacctta tatgctggaa gtagatttga tagaaatgaa aaagaaatag tagttcctga
    16501 ttttagaaag ttaaataagg aagaggcaga agaaatttta aaatccttag gattaaaggg
    16561 agaatttgaa ggagaaggtg aaattaagga acagagcata agtgcaggag aaataataaa
    16621 aagtggatat aagattaagt ttaaattaga aggataaaac tttaaaataa agagttatat
    16681 caaataactt ataat
//
LOCUS       DAEVGI010000043        16679 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEVGI010000043 DAEVGI010000000
VERSION     DAEVGI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 16679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16679
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.043"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(116..2746)
                     /locus_tag="K8M77_001205"
     CDS             complement(116..2746)
                     /locus_tag="K8M77_001205"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBI7085712.1"
                     /translation="MEKKQYVYLFNEGNASMKNLLGGKGANLSEMTILGIPVPQGFTV
                     TTEACNKYYEDDKKISQDIIEEIENKMSELEKITGKKFGSLENPLLVSVRSGARVSMP
                     GMMDTILNLGLNDESVEAMAKLTNNPRFAYDSYRRFIQMFADVVMGVEKRLFEDLLDE
                     VKEEKGYKIDTDLTAEDLKDLVVKFKALYKKEKGEDFPSNPKEQLIEAVTAVFRSWNN
                     PRAIVYRRLNDIPGEWGTAVNVQEMVFGNKGETSGTGVAFSRNPANGDNELYGEYLMN
                     AQGEDVVAGIRTPEPISHLEAQNPEIYKQFTDIVNTLEKHYRDMQDMEFTIEDGKLYF
                     LQTRNGKRTAQAALKIAVDLVEEGMLTKEEAILKVEPKQLDTLLHPAFASDGLKEAKI
                     VAKGLPASPGAACGKIAFTAEEAKERKANGEKVVLVRLETSPEDIEGMIAAEGILTVR
                     GGMTSHAAVVARGMGTCCVAGCGELKVNEEARTLEVNGQVLTFDDYISIDGSTGHIYA
                     EQVKTVSPEITGHFATFMGWADEIRKLKVRTNADTPRDTKQAVEFGAEGIGLCRTEHM
                     FFAEDRILAVREMILAKNEDSRRVALEKLLPMQREDFIGMYEALEERPATIRFLDPPL
                     HEFLPNEEEDINALAKEIGVSPAEIKNVVAELHEFNPMMGHRGCRLAVSYPEIAEMQT
                     RAVIEAAIEVKKNKGYNIVPEIMIPLIGEIKELKYVKNVVVETAKTVMEEKGIQLDYK
                     VGTMIEIPRAALTADKIAEEAEFFSFGTNDLTQMTFGFSRDDAAKFLKDYYEKGIYEQ
                     DPFAKLDQEGVGELMRIACEKGKATRPDIKLGICGEHGGDPSSVEFCHNLGLNYVSCS
                     PYRVPLARLAAAQAQVKNKR"
     gene            complement(2847..3479)
                     /locus_tag="K8M77_001206"
     CDS             complement(2847..3479)
                     /locus_tag="K8M77_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7085713.1"
                     /translation="MKLTPRQEEIVRLVKEGQPITSEALAGKLGVTRAALRPDLAILT
                     MIGVLDARPKVGYVYSDKPSNSFIYEKIKNIKVKDIMSKPVTVCEETMLHDAIVHLFL
                     NDVGTMFVENGGVLTGAVSRKDFLKVAIGGTDIYKVPVGVIMTRMPNIVCAVEEDCAF
                     DVAKKIIEHEIDSVPVVKRIKENEGKERLEIIGKISKTNITKLFVKLGEN"
     gene            complement(3684..4307)
                     /locus_tag="K8M77_001207"
     CDS             complement(3684..4307)
                     /locus_tag="K8M77_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBI7085714.1"
                     /translation="MSEITLEKVDQVIERTYATYAEAKEALEACDGDVVNALIYIEKK
                     REEESKVEEKVMEEKAESFEDIKKFLKDLIDKGNVSRIRILKDEKALTDIPVNAGIAA
                     GAIAVIFPSILALSVVAAIATKITIEITKADGSVEIVNKIVKNAANDIKGKAFGLKEK
                     AFSVANDMKEKRENGKTNENKTNKFHKHNKLENETSFTYTVKFDDVD"
     gene            complement(4313..5053)
                     /gene="recO"
                     /locus_tag="K8M77_001208"
     CDS             complement(4313..5053)
                     /gene="recO"
                     /locus_tag="K8M77_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034833182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBI7085715.1"
                     /translation="MALIESGGVIIKAQDYKENDKLLWIFTEKMGKITAIAKGAKKSK
                     SKLFSLTHPLCYGDYLLFKGKGLYRLSEGKIKTSFQTSLTDLEKLTYASYLCELIDIS
                     LQDEEENFNLYKEFITCLYLINTEAISYELLIRAFELKLLKYTGYGLRFDNCVFCKNK
                     LSVSNYISLRYFGGVCDKCPKEHGLYINKATYNALRFLNNTSLDKVYRLTLTEEVKAE
                     LFKVTSFIISSVYSRKPKSLEMLKFIKE"
     gene            complement(5066..5956)
                     /gene="era"
                     /locus_tag="K8M77_001209"
     CDS             complement(5066..5956)
                     /gene="era"
                     /locus_tag="K8M77_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBI7085716.1"
                     /translation="MFKSGFITIVGRPNVGKSTLTNLLMGEKLSIVSNKPQTTRNNIQ
                     TILTGDDYQMIFVDTPGIHKPKHKLGEYMVNSATDSIKDVDLVLFLSNPCEEVGRGDK
                     FIIEQLKNQKAPVIFVLNKVDESSPEKVAKTLELFSKEYDFAEMIPISAMKAKNTDKL
                     LELMVKYLPEGPKYYPDDMITDVQERFVVAEIVREKALKNLSQEVPHGIAVDVIQMKQ
                     DDNGKYNIEVDLICEKASHKGIIIGKNGQTLKKIGSTARYELERFLRAKVNIKIWVKV
                     RKEWRDNTSLLKELGYKKLK"
     gene            complement(5965..6363)
                     /locus_tag="K8M77_001210"
     CDS             complement(5965..6363)
                     /locus_tag="K8M77_001210"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBI7085717.1"
                     /translation="MKKELIKKALEARELAYCPYSNFKVGAAALFEDGKIYTGCNIEN
                     ASFGATNCAERTAIFKGVSEGARVLKAMALVGDLNSYTYPCGICRQVIVEFAEDKDIK
                     IFIVKSEDDYIETTLDEILPGSFTKKDLEK"
     gene            complement(6407..7105)
                     /locus_tag="K8M77_001211"
     CDS             complement(6407..7105)
                     /locus_tag="K8M77_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBI7085718.1"
                     /translation="MKVRKLIDSFNYAIDGIIYALRTQRNMRIHFIVALIILSACFVY
                     DISKIEFIILAITITMVIAAELVNTAIESAIDLSTNYYHPLAKIAKNTAAGAVLITAI
                     NSVLVGYIIFWDELTNITFRIMDRVKETEPYIIFIVLAIVCIVVVAAKAYFGEGTPLK
                     GGMPSGHSAIAFSIATAISLISNSHIIIILSYLMALITAQSRVDSEVHSVWEVIAGAI
                     LGTLITILIFKIFS"
     gene            complement(7133..7639)
                     /gene="ybeY"
                     /locus_tag="K8M77_001212"
     CDS             complement(7133..7639)
                     /gene="ybeY"
                     /locus_tag="K8M77_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBI7085719.1"
                     /translation="MIFIDNRQNKFDVTEELTKKLEEVISFVLKEEKVKEDCEVSLVF
                     VDNEEIRGINNETRGIDKATDVLSFPMIDYPEDKVYKDVYLEHEFDKCYFDGDELILG
                     DIVLSLERTKEQSIEFNHSFEREACYLVTHSVLHLLGYDHMEEEEKARMRGREEELLG
                     KLNITRES"
     gene            complement(7641..9695)
                     /locus_tag="K8M77_001213"
     CDS             complement(7641..9695)
                     /locus_tag="K8M77_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBI7085720.1"
                     /translation="MGKLKEKIFDIFKDYKKIILYIITFIVLYCTLMTSIITRKYDLK
                     VGDIPKSDIKAHREIIDESATEARKKEAEEKVDKQYSLRTDVQKQSEEKIKGFFSSVE
                     KVLAQDKSEEEKAKLIPRAPFKITDEQANKIASMNAQSTKNLESVCIDGLNKAYEAPI
                     EDVNEQDLKDAKKEYTDFISSSDLSDSEKAIALNFVNVVEPNFFYDKEKTDELIKETL
                     KQVPPVMIKKNQIVVSEGEPVTAHQLELLGTLGLLSDSASAICIYIALGVLVIIVMYL
                     QYGYIHKYYTAINKEFSKIVMISILNVFPVILARLFGMMSNYIIPLACMPMLITLLLN
                     YKISLVFSMLNVILIGGAVGFNPNIIILAILNVVLGGTLLRKMQQRNDILYSSITVAV
                     LSSILTFSVGTLTTNNFMEILADSTFAAAGAILSGILTIGVLPFFESTFDIVTNAKLL
                     ELSNPNNPLLKKLLMEAPGTYHHSILVANLAELAAEQVGGNPLLARIGAYYHDVGKTK
                     RPYFFRENQFGKKNPHDRLKPEVSSKIIISHVKDGSELAKEYNLPKTIHDFIVTHHGE
                     TLVKYFYLTVKNNSENPDEVKEEDFKYPGPKPMSKEQGIVMLADSTEAAVRSINEPTE
                     EKIEKMVNNIIDDKLASGQLDNCDLTLRDISKIKKCFLKALNGIHHERIEYPDDNKKE
                     KK"
     gene            complement(9732..10868)
                     /gene="yqfD"
                     /locus_tag="K8M77_001214"
     CDS             complement(9732..10868)
                     /gene="yqfD"
                     /locus_tag="K8M77_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBI7085721.1"
                     /translation="MNLEFLKKGQVKVEIKTFDINKLLNVLWRNGINVENVRKIDVVT
                     VTLNVDYSDYGKLKSYVKRLNGKVKIISSKGMIFFLSRAKKSISIFVGVLIFLIGLYI
                     YSGFIWRIDIETKKNIAPFEIRTMLNDFDIKPGVKKSSVNVYGLERKLENGHGDIMWV
                     NARIEGGTLKIKVEEKVSPKIKEENENKEFKNIVASMDGEIKRIYTTSGTAVVKEGDV
                     VKKGDILIIPQQGIEGGEYEVDAEGEVTANTFYEKTIELQVQGKKEERTGEVDSDIYL
                     EIMGKKIYLKKPTKEFDKYDKIESTGKFINRNLYYEKIDKDIVDDKETIINKAVELMK
                     NSINKEISKQSKIVENIITTEDLGEGKIRLKVLFVVEQNIALSY"
     gene            complement(10870..11151)
                     /gene="yqfC"
                     /locus_tag="K8M77_001215"
     CDS             complement(10870..11151)
                     /gene="yqfC"
                     /locus_tag="K8M77_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HBI7085722.1"
                     /translation="MGDKLRQVTNRIAEELDLPKEIINGEPKIVVHGKSEIIIENHKG
                     IEVFKENEIKINSNLGVIIIEGTKLEIRFIGTETIALTGRLKNLSFEEA"
     gene            complement(11375..11824)
                     /locus_tag="K8M77_001216"
     CDS             complement(11375..11824)
                     /locus_tag="K8M77_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBI7085723.1"
                     /translation="MPTIKERLQEDWKQALKAKDKFKAGVLSTAKSAILLVEKTDGVK
                     LEDEQVIEILAREIKQRREAALEFEKGNRQDLVDQNNSEIAILLDYLPQQLSEEEIKD
                     IIKQAAEEVGANSMKDMGKVMSKVRPQTVGRADGKLVSQLVKEYLNK"
     gene            complement(11874..12050)
                     /locus_tag="K8M77_001217"
     CDS             complement(11874..12050)
                     /locus_tag="K8M77_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBI7085724.1"
                     /translation="MSEIRVKENESLEQALRRFKRQCARAGVLSEVRKREHYEKPSVK
                     RKKKSEAARKRKFK"
     gene            complement(12170..12514)
                     /locus_tag="K8M77_001218"
     CDS             complement(12170..12514)
                     /locus_tag="K8M77_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBI7085725.1"
                     /translation="MSDCIFCKIVAGEIPSKKIYEDDKVLAFHDISPEAPVHFLVIPK
                     EHIASLNEVNEENAEVFAHIFKTINKLVKEQEVAEDGYRVVTNCGEQGGQTVGHIHFH
                     VLGGRNLNWPPG"
     gene            complement(12583..13887)
                     /gene="mtaB"
                     /locus_tag="K8M77_001219"
     CDS             complement(12583..13887)
                     /gene="mtaB"
                     /locus_tag="K8M77_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBI7085726.1"
                     /translation="MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVN
                     TCSVTNMGDKKSRQIIGRARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKG
                     EIVYYVNKARDEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCL
                     IPYTRGSVCSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEI
                     EKIDGIERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTA
                     KEYEDIVNLLRDKIEDVSITTDVIVGFPGETDEEFKETYEFLKRIALSKTHIFKYSPR
                     KGTRAENMENQVDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGV
                     YTGYTRNYIKVNAKADCNVSGKILNVKINSFEGEVAEGEIVL"
     gene            complement(13887..14639)
                     /locus_tag="K8M77_001220"
     CDS             complement(13887..14639)
                     /locus_tag="K8M77_001220"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBI7085727.1"
                     /translation="MHKFFTPAHMFSDTHAKITGEDVKHIYKVLRVKPGEKVVVNDLE
                     SEEFLGEVEEVNKQEVSIKILEKLDVNNESNLNITLFQGMPKAAKMDLIVQKCVELGV
                     SEIIPTITDRVDVKLKGEFKKLDRLNKIALEACKQSKRTVIPQVLEPIDFKGVLEKID
                     ELDLMVIPYENATGYGIRAMVNSIENKDSIKNVGIMVGPEGGFEESEIEALKEKGAHI
                     VTLGPRILRTETAGFTCISLIQYELGDLGGNI"
     gene            complement(14662..15603)
                     /gene="prmA"
                     /locus_tag="K8M77_001221"
     CDS             complement(14662..15603)
                     /gene="prmA"
                     /locus_tag="K8M77_001221"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBI7085728.1"
                     /translation="MEGTWIEVRVITKSEALEPISGIFYGLDCKGVAIEDPNDILGRE
                     QGPLTWDFADINILEHKGKVAVVKGYFSEEDNIDDVIAYVKERVEELKESGLDVGEGT
                     VEAEKMFEEDWANNWKKYYKPIKIGEKIVIKPIWEEYEPTGEEMVVELDPGMAFGTGD
                     HETTRMCVQALDKYVKADTTVFDIGTGSGILALVASKLGAKHVVGVDLDPVAVDSAKE
                     NISFNNVDNIEVLYGNLLDVVDGKADIVVANIIAEIICILVDDVKKALNKDGIFITSG
                     IIHERRQMVIDKLEQEGFEVMEVNKDGEWNCIVAKLK"
     gene            complement(15887..16243)
                     /locus_tag="K8M77_001222"
     CDS             complement(15887..16243)
                     /locus_tag="K8M77_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7085729.1"
                     /translation="MFFEVNDREPIYLQIIRHIKQSLVIGNLKPGDHIPSRRELAEML
                     KVNPNTVQRAYRDMETMGLIETIRNKPSTITEDEEILKNIRKELVEDALDGFIESMKA
                     IKLTKEEVLEIIRERY"
     gene            complement(16245..>16679)
                     /locus_tag="K8M77_001223"
     CDS             complement(16245..>16679)
                     /locus_tag="K8M77_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085730.1"
                     /translation="LNVNTREIKRYNPDDKIRNEIKESSFDPNGKGGTMFEAYGKIYI
                     TQTSDEKTSVLVFDTNTKEFKFYNDLIENERLKRYGAEVRDLGKFIIVGNKVIANFVN
                     VRDDGFVSGAYLSVIDIPSKNPVYIGELECGYLSDIKITGGK"
BASE COUNT         4894 a         3347 c         1732 g         6706 t
ORIGIN      
        1 aatataaatt gtacataaac tcttttaata catacaaaaa gatgaattca aactaatgaa
       61 tccatcttaa ctttaaattt attttcaata tttttttaat ttaaattatt atttattatc
      121 ttttattttt aacttgtgct tgtgcagctg caagtcttgc taatggaact ctataaggtg
      181 aacaacttac gtaatttaat cctaagttgt ggcaaaattc aacagatgaa ggatctccac
      241 cgtgttctcc acatatacct aatttaatat caggtcttgt tgccttacct ttttcacaag
      301 caattctcat aagctctcca acgccttctt ggtctaattt agcaaatgga tcttgctcat
      361 atattccttt ttcatagtaa tcttttaaga atttagctgc atcatctctt gagaatccaa
      421 aggtcatttg agttaaatca tttgttccaa aggagaagaa ctcagcctct tctgctatct
      481 tatcagctgt taaagcagct cttggaattt caatcattgt tccaacctta taatctaatt
      541 gaataccttt ctcttccatt actgtcttag ctgtttcaac aactacattt ttaacatatt
      601 ttaattcttt tatttcacct attaaaggaa tcattatttc aggaactata ttgtatcctt
      661 tattcttttt aacttctatg gcagcctcta tgacagctct tgtttgcatt tcagctatct
      721 ctggataaga tactgctaaa cgacatcctc tatgccccat cattgggtta aattcatgta
      781 attcagcaac tacattctta atctcagctg gacttactcc aatttcttta gctaaggcat
      841 ttatatcttc ctcttcattt ggtaagaatt catgtagagg tggatctaag aatcttattg
      901 tagctggtct ttcttctaaa gcttcataca ttcctataaa gtcttctctt tgcattggta
      961 gaagtttttc taatgcaact cttctactat cctcattctt agctaagatc atttctctaa
     1021 cagctaaaat tctatcctca gcaaagaaca tatgctctgt tctacataat ccaatacctt
     1081 ctgcaccaaa ttcaacagct tgtttagtat ctcttggtgt atcagcatta gttctaacct
     1141 ttaattttct tatttcatca gcccatccca taaatgtagc aaaatgtcct gtaatttctg
     1201 gagatactgt cttaacttgt tcagcataga tgtgtcctgt tgaaccatct attgaaatat
     1261 aatcatcaaa ggttaatact tgtccattaa cttctaaagt tctagcttct tcattaactt
     1321 ttaattctcc acatcctgct acgcagcaag tacccattcc tcttgctaca acggctgcat
     1381 gagaagtcat tcctcctcta acagttaata taccttctgc ggcaatcata ccttctatat
     1441 cttctggtga agtctctaat cttactaata caaccttttc accatttgct tttctttcct
     1501 tagcttcttc agctgtgaaa gctattttac cacatgcagc tcctggtgat gccggtaatc
     1561 cctttgcaac tattttagct tcttttaatc catcagatgc aaaagcaggg tgaagtaatg
     1621 tatctaattg tttaggctca acttttaata tagcctcttc cttagttaac attccttctt
     1681 caactaaatc aacagctatt tttaaagctg cttgagcagt tctcttacca tttctagttt
     1741 gaaggaaata taactttcca tcttctattg tgaattccat gtcttgcata tctctataat
     1801 gcttttctaa tgtattaact atatctgtaa attgcttata tatttcagga ttttgagctt
     1861 ctaaatgact tattggctct ggtgttctta tacctgcaac aacatcctca ccttgagcat
     1921 tcattaaata ctcaccatat aattcattat ccccattagc tgggtttctt gaaaatgcaa
     1981 ctccagttcc tgaagtttct cccttatttc caaataccat ctcttgaacg ttaactgctg
     2041 taccccattc tcctggaata tcatttaatc ttctataaac aattgctctt ggattattcc
     2101 atgatctaaa tacagctgta actgcctcta taagttgttc ttttggatta cttggaaaat
     2161 cttctccctt ttccttttta taaagcgctt taaattttac tactaaatct tttaaatcct
     2221 ctgctgttaa atcagtatct attttataac ctttttcttc cttaacttcg tctagtaaat
     2281 cttcaaaaag tcttttttca acacccatta caacatcagc aaacatttga ataaatcttc
     2341 tatatgaatc ataagcaaat ctcggattat ttgttaattt agccatagct tctactgatt
     2401 catcatttaa tccaaggttt agaattgtat ccatcatacc tggcattgaa actcttgcac
     2461 ctgatctaac tgatactaat aatggatttt caagagaacc aaattttttg ccagttattt
     2521 tttctaactc agacatttta ttttcaatct cttcaataat atcttgagaa atctttttgt
     2581 catcttcata atacttatta catgcttcag ttgttactgt aaatccttgt ggaactggta
     2641 ttcctaaaat agtcatctcg gataagttag caccttttcc acctaataaa tttttcatac
     2701 ttgcgttacc ttcattaaaa agataaacat actgcttttt ttccatctca atactcctcc
     2761 gttcaatact atagttgaat ttttatatac taacagagta ttacccccga tagttaccca
     2821 tttaatcttt ctttttaacc ataaatttaa ttttctccaa gcttaacaaa caactttgtt
     2881 atgttagtct tagaaatttt tcctatgatc tctaatctct cttttccctc attttcttta
     2941 attcttttta caactggtac ggagtctatt tcatgttcta ttatcttttt tgctacatca
     3001 aaagcgcaat cttcttctac agcacatact atgttaggca ttcttgtcat tataacacca
     3061 acaggaacct tataaatatc tgttcctcct atagcgacct ttaaaaagtc ttttctggaa
     3121 acagctcctg ttaaaactcc tccattttct acaaacatag ttcctacatc atttaaaaat
     3181 agatgtacta tagcatcatg aagcatagtt tcttcacata ctgtaaccgg tttagacatt
     3241 atgtctttaa cctttatatt ttttattttt tcatagatga aactgttcga aggtttatct
     3301 gaataaacat acccaacctt tggtcttgca tctaatacgc caatcattgt aagtattgca
     3361 agatctggtc ttaaggctgc tcttgtaacc cctaactttc ctgctaaagc ttctgaagtt
     3421 attggttgac cctcttttac aagtctaact atttcttcct gcctcggtgt taatttcaat
     3481 ttgaatgctc cttccttaaa taaactagga acaaaaagga ttttttatat taaagtgatt
     3541 tttttatata ttaattgtaa cacaacctaa atctttttag tatagcaaat tttctgaaaa
     3601 atcattcaaa tctgataatt atcacttaaa atcatatagt tttttcctct ttattccata
     3661 ttcctttatt ttacatttat ttgctagtca acatcatcaa attttaccgt atatgtaaaa
     3721 cttgtttcat tttccaactt attatgttta tgaaatttgt ttgttttatt ctcatttgtt
     3781 tttccatttt ctcttttttc tttcatgtca tttgctacac taaaagcttt ttcctttagt
     3841 ccaaatgctt tccccttaat atcattagct gcatttttga ctattttatt aacaatctca
     3901 acacttccat cagccttagt tatttctatt gtaatctttg ttgcaatagc ggctaccaca
     3961 cttagtgcta atatacttgg gaatattacg gcaatggccc ctgcagctat cccagcatta
     4021 actggaatat cagttaatgc tttttcatcc ttaagaattc ttattctaga tacatttccc
     4081 ttatcaatta aatcctttaa gaattttttt atatcttcaa agctttctgc cttttcttcc
     4141 attacctttt cttctacttt actttcttct tctctttttt tctcaatata tattaatgca
     4201 ttaacaacat caccatcaca agcttctaat gcctctttag cttctgcgta agtagcatat
     4261 gttctctcta ttacctggtc tactttttct aatgttattt cactcatgta aatcactcct
     4321 ttataaactt taacatttct aaacttttag gttttcttga atatacagaa gatattataa
     4381 aactagtaac cttaaaaagt tctgctttaa cctcttctgt taatgttaat ctatatactt
     4441 tatccaaaga agtattattt aagaatctta aggcattata tgtggcttta tttatgtaaa
     4501 gcccatgttc cttgggacat ttatcacaaa cacctccaaa atatcttaaa ctaatgtagt
     4561 ttgaaacact taatttattt ttacaaaata cacaattatc aaatcttaat ccatacccag
     4621 tgtattttaa taatttaagc tcaaaagctc ttattaatag ttcataactt atagcctcag
     4681 tatttattaa ataaagacag gttataaact ctttatataa attaaaattt tcttcttcat
     4741 cctgtagtga tatatcaata agctcacata agtaagatgc ataagtaagc ttttctaggt
     4801 cagttaaaga tgtctgaaag gaagttttta ttttcccttc agataaccta tataaaccct
     4861 ttcccttgaa taataaataa tcgccatagc ataaagggtg cgttaatgaa aagagtttac
     4921 ttttactttt ctttgcaccc ttagctatag ctgttatctt acccattttc tcggtaaaaa
     4981 tccaaagcaa tttatcgttc tctttataat cttgagcctt aatgataact ccaccactct
     5041 ctataagtgc cagaagctat ccctcctatt taagtttttt ataacctaat tcctttaata
     5101 gtgaagtatt atctctccac tcttttctaa ctttcaccca tatttttata tttacttttg
     5161 ctcttaagaa tctttctaat tcatatctag ctgtagaacc aatcttctta agtgtttgac
     5221 cattttttcc aattattatt cctttatgag atgctttttc acatattaaa tctacttcta
     5281 tgttatactt accattatca tcttgtttca tttgtataac atctacagca ataccatgag
     5341 gaacttcttg gcttaaattt ttaagtgctt tttctctaac tatctctgct acaacaaatc
     5401 tttcttgaac atctgttatc atatcatctg gataatattt aggaccctct ggtagatatt
     5461 tgaccataag ttctaaaagt ttatctgtat ttttagcttt catagctgat attggaatca
     5521 tttctgcaaa gtcatattct tttgagaata attctaaagt tttagcaacc ttttctggtg
     5581 aactctcatc aactttgttt aatacaaata taacaggagc tttttgattt ttaagctgct
     5641 ctattataaa cttatctcct ctaccaactt cctcacatgg attacttaaa aataatacta
     5701 aatctacatc ttttatagaa tctgtagcac tatttaccat atactcacct agtttatgtt
     5761 taggcttatg tatccctgga gtatcaacga aaatcatttg atagtcatct ccagttaata
     5821 ttgtttgaat attatttctt gttgtttgtg gtttattaga aacaattgaa agtttttctc
     5881 ccattaataa gtttgttaat gttgacttac ctacatttgg tctaccaaca attgttataa
     5941 atcctgattt aaacatattt cctcctattt ttctagatcc tttttagtaa aagatccagg
     6001 taaaatttca tctaatgttg tctctatata atcatcttca ctttttacta tgaatatttt
     6061 tatatcttta tcttctgcaa actctactat tacttgtctg catataccac atggatatgt
     6121 gtatgaattt aaatctccta ctaaagccat agcctttaag actcttgcac cttctgaaac
     6181 ccctttaaat atagcagttc tctcagcaca gtttgttgct ccaaatgatg cattttcaat
     6241 attgcatcct gtatatattt taccatcctc aaataaagca gctgcaccta ccttaaaatt
     6301 tgaatatgga caatatgcca attctcttgc ctctaaagct ttttttataa gttctttttt
     6361 cataataact catcctccat cctaaaattt aaatactaaa aatttatcaa ctaaatattt
     6421 taaaaattaa aattgttata agcgttccta atattgctcc tgcaataact tcccatacgc
     6481 tatggacttc tgaatccact ctgctctggg ccgttattaa agccattaaa taactaagta
     6541 taattataat atgagaatta cttataagtg atatagccgt agctatagaa aaggctatag
     6601 cactgtgtcc tgaaggcatt ccacccttta atggggttcc ttctccaaaa taagcctttg
     6661 cagcaaccac aactatgcat actatggcta atacaataaa tataatataa ggctctgttt
     6721 cttttactct atccataatt ctaaaagtta tattggtaag ctcatcccaa aatataatat
     6781 accccactaa gacagaattt attgcagtta taagtactgc cccagctgct gtatttttag
     6841 ctattttagc taaaggatga tagtaatttg tacttagatc tatagcgctt tctatagcag
     6901 tattaactaa ttctgctgct attaccatgg ttattgttat ggctagtatt ataaactcta
     6961 ttttgcttat atcataaaca aaacaagctg ataatataat taaagctact ataaaatgta
     7021 ttctcatgtt tctttgagtt cttaaggcat atattatacc atctattgcg taattaaagc
     7081 tatcaataag ttttcttacc ttcataatat tctcctaaac tattctcata ccttagcttt
     7141 cccttgttat atttaactta ccaagtaact cctcttctct tcctctcatt ctagcttttt
     7201 cttcctcttc catatggtca taacctaaaa ggtgtaaaac tgagtgagtt actaagtagc
     7261 aagcctctct ttcaaatgag tgattaaact caatactttg ttctttagtt ctttctagag
     7321 ataaaactat atctcctaaa ataagttcat ctccatcaaa atagcattta tcaaattcat
     7381 gctctaaata aacatcttta taaactttgt cctctggata atcaatcatt ggaaatgata
     7441 aaacatcagt tgctttatct attcctctag tttcattgtt tattcctcta atttcttcgt
     7501 tgtctacaaa gactaaagaa acttcacaat cctctttaac tttttcttct tttagcacaa
     7561 aagaaataac ttcttctaac ttcttagtta attcttccgt aacatcaaat ttattctgtc
     7621 tattatctat gaatatcatt ttattttttc tccttcttgt tatcatctgg atattctatt
     7681 ctttcatgat gtattccatt taatgccttt aggaaacatt tttttatctt acttatatct
     7741 ctaagagtaa gatcacaatt gtctagttgt ccagatgcta acttatcatc aataatatta
     7801 ttgaccattt tttcaatttt ctcttcagta ggttcattaa ttgatcttac agctgcctct
     7861 gttgaatctg ctagcataac aattccttgt tctttactca taggttttgg tcctggatat
     7921 ttaaaatcct cttccttaac ctcatctgga ttttcagagt tattttttac tgttagataa
     7981 aaatatttaa ctaaagtctc cccatgatgg gtaactataa aatcatgtat agtcttaggt
     8041 aaattatatt ccttggctaa ttctgatcca tctttaacgt gagatattat tattttacta
     8101 cttacttctg gttttaacct atcatgtgga tttttctttc caaactgatt ttctctaaag
     8161 aaatatggtc tcttagtttt accaacatca tgataataag ctcctattct agctaatagc
     8221 ggatttcctc caacttgctc agcagctaac tctgctaagt ttgctactaa aatactatga
     8281 tgatatgttc ctggagcctc cataagtaat tttttaagta atggattatt aggattagat
     8341 aattccaata acttagcatt agttactata tcaaatgttg actcaaagaa cggtaaaaca
     8401 cctattgtaa gtattccaga aagtatagct cctgccgctg caaatgtact atctgcaagt
     8461 atttccataa agttattagt tgttaaagta ccaacactaa aagttaatat tgaacttaaa
     8521 actgcaacgg ttattgaaga atataatata tcatttctct gttgcatttt tctaagcaat
     8581 gttcctccta aaacaacatt taaaatagca agaattatta tattaggatt aaatcctact
     8641 gctcctccta ttaaaattac atttaacata ctaaatacta gtgatatttt ataatttagt
     8701 agaagggtta taagcatagg catacaggct agtggtatta tatagtttga catcatacca
     8761 aaaagtctag ctagtattac tggaaataca tttaatatac ttatcataac tatttttgaa
     8821 aactctttat ttatagctgt ataatattta tgtatgtatc catactgaag atacataact
     8881 attattacta aaacccctag agcaatataa atacatattg ctgaagctga atcgcttaat
     8941 aatcctaaag ttcctaaaag ctctaattgg tgcgctgtaa ccggctcacc ttcacttact
     9001 actatttgat ttttctttat cataactggt ggaacttgct ttaaggtttc ttttatcaat
     9061 tcatcagttt tttctttatc atagaaaaaa tttggttcta ctacattaac aaaatttaat
     9121 gcaatagcct tctctgaatc acttaaatca ctggaagata taaaatctgt atattccttt
     9181 tttgcatctt ttaaatcctg ctcatttaca tcttcaattg gagcctcata tgctttattt
     9241 agaccatcta tgcatacact ttctaaattc ttagtacttt gcgcattcat tgaggcaatt
     9301 ttatttgcct gttcatctgt tattttaaat ggtgctctag gaataagctt agccttttct
     9361 tcttccgatt tatcttgagc taaaactttt tcaactgaag aaaagaaacc ctttatcttt
     9421 tcttcacttt gcttctgaac atctgtcctt agagaatatt gtttatcaac cttttcttca
     9481 gcttcttttt ttcttgcttc agtagctgat tcatcaataa tttccctatg tgccttaatg
     9541 tctgatttag gtatatctcc tacctttaaa tcatactttc ttgttattat actagtcatc
     9601 aaggtgcagt ataaaactat aaaggttatt atatataaga ttattttttt gtaatcttta
     9661 aatatatcaa atattttttc cttcaatttc cccataatta ctcatcttct cctcattagg
     9721 tcctaattta tctagtaact taaagctata ttttgttcta ctacgaaaag aacctttaat
     9781 ctaatcttcc cttcccctaa atcctcagtt gtaattatat tctcaactat ttttgattgc
     9841 ttacttattt ctttgtttat tgagtttttc attaactcaa cagctttatt tattatggtt
     9901 tccttatcat caactatgtc tttatctatt ttctcatagt ataagtttct atttataaac
     9961 tttcctgtac tttctatttt atcatactta tcaaactctt tagtaggctt ttttagataa
    10021 attttctttc ccattatttc taagtaaata tcactatcca cctctcctgt tctctcctct
    10081 tttttgccct gaacttgcaa ttctatagtc ttttcataaa aagtattcgc agtaacttct
    10141 ccttcagcat caacttcata ttcacctcct tctattcctt gctgtggtat tattaaaata
    10201 tctccctttt taaccacatc tccttccttt acaacagctg tacctgaagt tgtgtatatt
    10261 ctctttattt ccccatccat agatgctact atattcttaa actctttatt ttcattttct
    10321 tcttttattt ttggactaac cttttcctca acctttattt ttaaagttcc accctctata
    10381 cgagcattta cccacattat gtctccatgt ccattttcta attttctttc taatccataa
    10441 acatttacac tacttttctt aactcctggt ttaatatcaa aatcatttaa catggttctt
    10501 atctcaaaag gagctatatt tttctttgtc tctatatcta ttcgccatat aaaaccagaa
    10561 tagatatata agcctattaa aaatattaaa actcctacaa atatgcttat agacttttta
    10621 gctctagata agaaaaatat cattcccttt gatgatatta ttttcacctt tccattaagt
    10681 cttttaacat agctttttaa ttttccataa tcagaataat ccacatttaa agttacagtt
    10741 actacatcta ttttccttac attttcaaca tttataccgt ttctccataa aacatttaaa
    10801 agtttattta tatcaaaagt ttttatttca acctttactt gccccttttt taaaaactcc
    10861 aaattcatct tatgcctcct caaaacttaa attcttaagt cttcctgtta aggcaatagt
    10921 ttcagtacct ataaatctta tctctaactt tgttccttcg attataataa cacctagatt
    10981 gctatttatt ttaatttcat tttccttaaa aacctctatt cccttatgat tttctataat
    11041 aatttcactt tttccgtgta caactatttt aggctcacca tttattatct ccttaggtaa
    11101 atctaattcc tctgcaattc tattagtgac ttgtcttaat ttatcaccca taactatacc
    11161 tcccataaaa tattttcata ctaaatctat gaaaatttat atttttttat tcttatataa
    11221 aaaactaagc aatataaaaa ggcatataca tagccctttt agctatgtat atgcctttta
    11281 aaacaattca aaaaattttc tttaaaaact tctaactatt aataaaatat ctttttaaaa
    11341 ttataaaaat aaaagagagt tctaatgaac tctcttactt atttaaatat tctttaacta
    11401 attgacttac aagtttacca tcagctctac caactgtttg cggtctaacc tttgacataa
    11461 cttttcccat gtctttcatg ctatttgcgc ccacttcttc agctgcttgc tttatgatat
    11521 cttttatttc ttcttcactt aactgctgag gaaggtaatc taacaagatt gcaatctcac
    11581 tattgttttg atcaactaga tcctgtctat ttcccttttc gaattcaagc gcagcttctc
    11641 ttctttgctt gatttctctt gctaatattt ctataacctg ttcatcctca agcttaacac
    11701 cgtcagtctt ttcgactaaa agtattgctg acttagcagt acttaaaact ccagccttga
    11761 acttatcctt agcctttaaa gcctgcttcc agtcttcttg aagtctttct ttaatagttg
    11821 gcatttgtat accttctttc aaaagaaatt attctataaa tttaaaccaa aaactatttg
    11881 aattttctct ttcttgcagc ttctgatttt ttctttctct ttacgcttgg cttttcgtag
    11941 tgttctcttt ttcttacttc tgaaagaact ccagctctag cgcattgtct cttaaatctt
    12001 cttaatgctt gctctaatga ttcgttttct tttactctga tttctgacat tctttatccc
    12061 tccctccgct agcgtaaatt attaatgtaa ttcacagcca tgggctaaat aacacacgct
    12121 atattataca acatagtttt ctttaatgtc aagaagttaa atctaaattt tagcctggtg
    12181 gccaatttaa atttcttcct ccaagaacgt ggaaatggat atgtcctact gtttgccctc
    12241 cttgctctcc gcaattagtg acaactctgt atccatcctc tgcaacttct tgttccttta
    12301 ctagtttatt aatagtcttg aaaatgtgtg caaatacttc tgcattttct tcattaactt
    12361 catttaaact agctatgtgt tctttaggaa taactaaaaa atgtactggt gcttctggac
    12421 ttatatcatg aaaagctaaa actttatcat cttcataaat ctttttgctt ggaatttctc
    12481 cagctacaat tttgcaaaaa atacaatctg acatgatttt cacctcctaa gcaatgaatc
    12541 tattatctat tataactcat tttcaatgta aaaatcttct ttttataata ctatttcgcc
    12601 ctctgctact tctccctcaa aagaattaat ctttacattt aatattttac ctgaaacgtt
    12661 gcaatcagcc ttggcattta cctttatata atttcttgta tatccagtgt aaactccact
    12721 tcctaattca acttcttctt caaataaaac atcaataact tcacctatat atttttcagc
    12781 aaaggctttt tcattaattc tatttaattc aataagtttt ttacttcttt cgtctttctt
    12841 atttccatca acttgatttt ccatattctc tgctctagta ccttttctag gactatattt
    12901 gaatatgtga gtttttgaaa gagctattct ctttaagaat tcataagtct ctttaaactc
    12961 ttcatcagtt tctccaggga aaccaacaat tacgtctgtt gttatagaaa catcttctat
    13021 tttatctctt aaaagattta ctatatcttc atactcttta gcagtgtatc ttctattcat
    13081 tctttttaaa gtagcatcac atccactttg aagtgataag tggaaatgag gacatagttt
    13141 tttaagagct aagattcttc ttacaacatc ctctgtaaag aatgtagggt ctattgaacc
    13201 tattctaact ctttctattc catcgatttt ttctatctct tctagtaaat caactaatgt
    13261 tactttttca tctaaatcaa cgccataaga tgctgtatga attcctgata atattatttc
    13321 tttaaatcca tgctctgcta agcttcttat ttcatctaaa accttcttag ggtcttttga
    13381 acaaacagaa cccctagtat atggaattaa gcaataagca cagaatctat tgcatccatc
    13441 ttgtattttt aagaaagctc tagttttatt attatactct tcaattttta ggtcctcaaa
    13501 aactttattc tttaaaacag cgccaacctt aacttgttgc ttgccttcat ctctagcctt
    13561 atttacataa taaactattt cacctttatt tcttgaacct aaaactacat ctacaccttc
    13621 aattcctgaa acctctgtag gtgctatttg agcataacat ccaactacag ctataatagc
    13681 ctctggattt tgtcttctag ctcttcctat tatttgtctt gatttcttat ctcccatgtt
    13741 agttacgcta caagtattaa ctacatacac atctgccatt tcattaaaat ctacaacttc
    13801 atagccttcc ctaacaaatt tttcagtcat cgcttctgat tcatatgtat taactctaca
    13861 ccctagtgta gcaaatgcaa ctttcattaa atatttcctc ctaaatcacc aagctcatac
    13921 tgaataagag aaatgcaggt aaatcctgct gtctctgttc ttaaaattct tggtcctaaa
    13981 gttacaatat gagctccttt ttcttttagg gcttctattt cactttcttc aaatccgcct
    14041 tctggcccaa ccattattcc aacatttttt atactatctt tattctctat agagttaacc
    14101 atagctctta ttccgtatcc tgtagcattt tcataaggaa taaccataag gtctaattca
    14161 tctatctttt ctaaaacccc tttaaagtct attggttcta atacttgtgg tattaccgtt
    14221 cttttacttt gtttacatgc ctctaaagcg attttattta atctatctaa ttttttaaat
    14281 tcacctttta atttaacgtc tactctatca gtgattgttg gaattatttc tgaaacacca
    14341 agttctacac atttttgaac tattaaatcc atttttgcag cctttggcat tccttgaaat
    14401 aaagtaatat ttaaattact ttcattgttt acatcaagct tctctaagat tttgatagaa
    14461 acctcttgtt tattaacctc ttctacctca cctaaaaact cttcactttc aaggtcattt
    14521 acaacaacct tctcacctgg ttttaccctt aaaaccttat aaatatgttt tacatcttcc
    14581 cctgttattt ttgcatgagt gtctgaaaac atatgcgctg gtgtaaaaaa cttatgcata
    14641 attttatact cccttactaa attattttaa cttagcaaca atacagttcc attctccatc
    14701 tttgttaact tccataactt caaaaccttc ttgttctaat ttatctataa ccatttgtct
    14761 tctctcatgt attatacctg aagttatgaa aattccatct ttatttaatg cttttttaac
    14821 atcatcaact agtatgcaga ttatttcagc tattatattt gcaacaacta tatcagcttt
    14881 tccatctaca acatctaaaa gatttccgta taatacttct atgttgtcaa cattgttaaa
    14941 tgatatattt tccttagctg aatctacagc aactgggtct aagtcaactc ctacaacatg
    15001 ttttgcacca agtttagaag caactaaagc aagtattcct gaacctgttc caatatcaaa
    15061 tacagtagta tcagccttaa catatttatc taaagcttga acacacattc ttgtagtttc
    15121 atgatcacca gtaccaaaag ccatacctgg gtctaattct actaccattt cttctccagt
    15181 aggctcatac tcttcccata ttggttttat aactattttt tctccaatct ttattggctt
    15241 atagtatttc ttccagttat tagcccagtc ttcttcaaac attttttctg cttcaactgt
    15301 tccttcacca acatctaatc ctgactcttt taactcttct actctttcct taacataagc
    15361 tattacgtca tcaatgttat cctcttctga gaaataacct ttaacaacgg caaccttccc
    15421 cttatgttct aatatgttta tatctgcaaa atcccaagtt aaaggtcctt gttctcttcc
    15481 taatatatca tttggatcct ctatggcaac acccttacag tctaatccgt aaaatattcc
    15541 tgatataggc tctaatgcct cacttttagt tattactcta acttcaatcc aagttccttc
    15601 catttatgta tcaatccttt cttatgctac tttgactaat aatttatttt acactttagt
    15661 gtagtatctg tctagcttat aagcattatt atgtttcata tgctctatta aatttctaag
    15721 ataaactctc tttatcatta tttttattat cacacataaa aaaaataaag gctatagtct
    15781 attattgaca taacctttaa acttaatctt atttaattcc tctaaatata aaattagaaa
    15841 ctatataaat tgtttaaaat ttatatagct tcttacattt agttatttaa tatctctctc
    15901 taataatttc taaaacttct tccttagtta actttatagc tttcatactt tctataaatc
    15961 catctaatgc atcctctaca agctcttttc ttatattttt taatatttct tcatcttctg
    16021 ttatagtact tggtttattt cttattgttt ctataagtcc catagtctcc atatctctat
    16081 atgctctttg aaccgtatta ggatttactt ttaacatttc tgcaagctcc ctccttgatg
    16141 gtatatgatc ccctggcttt aaatttccaa taactaaaga ttgttttata tgtcttatta
    16201 tttggagata aattggttcc ctatcattta cttcaaaaaa cattttattt acctcctgtt
    16261 atcttaatgt cacttaaata tccacattct aactcaccta tataaactgg atttttacta
    16321 ggtatatcta taactgataa ataagctcca cttacaaaac catcatctct aacattaaca
    16381 aaattagcta taactttatt tcctacaatt ataaatttac ctaaatctct aacttctgcc
    16441 ccatatctct ttaatctttc attttctata aggtcattat aaaatttaaa ctcttttgta
    16501 ttagtgtcaa acactaatac tgaagtcttc tcatctgagg tctgggtaat atatatttta
    16561 ccataagctt caaacatcgt accacctttt ccatttggat caaaggaaga ttcctttatt
    16621 tcatttctta ttttatcatc aggattatat ctttttattt ctctagtatt tacatttaa
//
LOCUS       DAEVGI010000044        16576 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEVGI010000044 DAEVGI010000000
VERSION     DAEVGI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 16576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16576
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.044"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(35..1564)
                     /locus_tag="K8M77_001224"
     CDS             complement(35..1564)
                     /locus_tag="K8M77_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBI7085731.1"
                     /translation="MKKAFGVLQRIGKALMLPVALLPAAGLLLGFGSMFQDPQFLSLV
                     PALNAGWFQLIATIMSDAGNIIFSNLALLFAVGVAVGLSEGDGVAGLAAIVGFLILNV
                     TMGIGGGITADVVASTKNGMYATVLGIPTLQTGVFGGIIMGVIAAALYKRFYKIELPS
                     YLGFFAGKRFVPIITAISALVLGGIMVFVWPPIQGALLYFSQNMISANQTLSALLFGI
                     IERALIPFGLHHIWYNPFWFQFGEYTNKAGQLIMGDNQIFFAQLRDGGPFTAGTFMTG
                     KFPFMMFGLPAAALAMYHEAKPGKKKIASGILFSAALTSFLTGITEPLEFAFLFVAPV
                     LFVIHCVLAGLSFMIMQLLNVKIGMTFSGGLLDFILLGVIPNRTRWWLVILVGLGFGV
                     IYYFLFRFFIRKFDLKTPGREDDDIFDDMDNQTVNDDLAAEILIALGGAGNINKLDAC
                     ITRLRVTVNDSSKVNKERLKELGAAGVMQVGENIQAIFGGKSDILKTQIREIMNGMGE
                     K"
     gene            complement(1735..2631)
                     /locus_tag="K8M77_001225"
     CDS             complement(1735..2631)
                     /locus_tag="K8M77_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBI7085732.1"
                     /translation="MRKKLVSIFLGVTMAAFFVGCQSAKKAENKKDNGKVDVTVSIEP
                     LREFTEIVGGDKVNVKTMVPNGTEPHDFEPKTQDLLELNKAKVFVYNGLGMEHWKEQV
                     LNTIENKDVKVVEASKGAEVLKEGDKVDPHLWLSLDGAKIEAQNIKDALVEVDSDNKS
                     YYEENFKKFAEKLDSLANEYREKFNGLANKDFVTGHAAFGYLCREFGLKQVSVENLFG
                     EGEITPQKMQEIVEFCKKNDIKTIFMPELASEKISQTLANEVGGKIEKIYTLESNEEG
                     ISYIDAMKENLSKIYNALSSQK"
     gene            complement(2857..5625)
                     /locus_tag="K8M77_001226"
     CDS             complement(2857..5625)
                     /locus_tag="K8M77_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7085733.1"
                     /translation="MIIKKINVKSFGKLKNKEIELKDGLNIIYGENESGKSTLQSFIK
                     VMLYGVCNKRGKKTLSDRAKFTPLGEGSFSGELLVEDNGEEILIDRSFGKTKKYDDGN
                     ILNNITGEKINKYSWQEPGKDIFNLSSEGFKNTLFISQMGCTVESSKQDDVLNKIINI
                     LECGADEVSYKKIKNNLEENKKSLTNSRKTGKLDLLRIKESELLEERYKRVKIHEENI
                     ENELYLKGLKEKRTDLNLKIEELELHKRNLKKSELKSEYENINEYLNKKEKLEEELKV
                     LLEELKDVQAICNLKKLNNLRDEFKNYEFLKESLKEKEDKIDKLKESIYEYNEKLKSY
                     GILNEISNNLETKLIELRIEDDRLRENYNVIQKVKEELYQLNEKKDDFLSHLNALKLN
                     DNLVLQIEKDLEKYNNEKIYPSINANLKEEKNIIEEENKSFSRKIFLFRVGEVISILL
                     CAFLFISYFKSENIITLALSILGMLLFIFLLAKSSLNKKEKNNISKRINEIDELINEK
                     KEIEVLKSKIDEYKQFLKVKDEYFIKETLEDYRFIKKELDILYVEIKEKEKSLILLKE
                     DKTKEKILSNEAFIDKLLRVTGAGSIDEILELSREKKSQEDLLIRKKSEFNEIKEDLN
                     QLNNRRLEIEEKIKAYLENTSLQSIPLHEVYSRLEEIEAKIEYKEGLKNKLNSVEENC
                     NLLLKGRDILEVKNGLSYEESEIKNEVYNSEEEILNEIKLKNNDLLNIEKEIKDVEYL
                     ISSKELTCRSLFLIDEEIAEVKERIKSLEKKLKGVNIATNYMEKAFKELQKSFGPVIN
                     KKVEDIFKDVTKGAYKDLRVSEDYNLVAKDTKSNKIMDASYLSSGTYDQIYLALRLGI
                     IDIIFEDKKVPIVLDESFTQYDDNRLKTMLDIIYKRVYKNQIILFTCQKREINILKDK
                     EKVNIIEL"
     gene            complement(5615..6754)
                     /locus_tag="K8M77_001227"
     CDS             complement(5615..6754)
                     /locus_tag="K8M77_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBI7085734.1"
                     /translation="MKKVKILHCSDIHFDTPFKELPKEIGTMRRAELRESFSKIINRG
                     IDEKVDLVLLAGDLFDNDTIEKSTLSFIKDQMDKLKKHNIRVFISAGNHDPYNKKSFY
                     NMVNLGENVHIFKDEIERVEIPELNTVVYGASFKEKYIKESKLKDFTPKEEDKDLVKI
                     MVLHGDLGNNETGEYNPLLFKEIEESKMDYIALGHIHKFSGIKRIGKTYYAYSGCPEG
                     RGFDEEGDKGVILGEVSLGALDLDFVKTNKREYFLKEIDISNCKTKEEVIDKILNTIS
                     KESRAKNLFKIILKGNIEESFKIDSDLIENLIRDKFFYCKIVNSTRMKIDIDEMSNEY
                     SIKGIFCSLIKDALESNEEDEEILDIAFRIGMESILDGEVNLNDN"
     gene            complement(7035..7217)
                     /locus_tag="K8M77_001228"
     CDS             complement(7035..7217)
                     /locus_tag="K8M77_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBI7085735.1"
                     /translation="MSKSLVPEAKNGLSKFKNEVARELGVPFSDYNGDLSSRQCGSVG
                     GEMVKRMVEAYESQIK"
     gene            complement(7448..7523)
                     /locus_tag="K8M77_001229"
     tRNA            complement(7448..7523)
                     /locus_tag="K8M77_001229"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7488..7490),aa:Trp,seq:cca)
     gene            complement(7552..7627)
                     /locus_tag="K8M77_001230"
     tRNA            complement(7552..7627)
                     /locus_tag="K8M77_001230"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7592..7594),aa:Trp,seq:cca)
     gene            complement(7889..8509)
                     /locus_tag="K8M77_001231"
     CDS             complement(7889..8509)
                     /locus_tag="K8M77_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBI7085736.1"
                     /translation="MLVKAIMTKKENLDLVDSNTKLKDALKIMEDNKFLSIPVVDGDK
                     FRGAVAKSSIYKYYFKNNLSKDEVLNNITVDEILKTEVPIINKSEHIEKAVSMLERMR
                     ISFVAVVDEFDNFQGILTHKAVFREFNNAFGLNKGERIVIRSYDVPGQLSKITKTISE
                     EGGDILSIVIVDTKSLTDVKEVIVRVKSIDVENIKKKLKESGFKLA"
     gene            8809..9273
                     /gene="tsaE"
                     /locus_tag="K8M77_001232"
     CDS             8809..9273
                     /gene="tsaE"
                     /locus_tag="K8M77_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBI7085737.1"
                     /translation="MEFIVDSVDKTMHIGRQLGSLVNKGDILCLTGDLGTGKTHISKG
                     IAEGLGIDEHITSPTFNIVNEYHSGRLTLYHFDVYRVNDPDEIEAIGFDEYIFGDGVS
                     LIEWANYIEELIPEEYIHIKIEKLPDMGENFRKISINGYGDRYNYIKEIEEC"
     gene            9267..9971
                     /gene="tsaB"
                     /locus_tag="K8M77_001233"
     CDS             9267..9971
                     /gene="tsaB"
                     /locus_tag="K8M77_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBI7085738.1"
                     /translation="MLVLAIDSSSSTATCALVSEKGVLGEINLNDKKQHSVLLMDLID
                     DLLKYNNLKIKDLDGFAISEGPGSFTGLRIGMATVKGLSFGSKKPSVCISSLDALAYN
                     ISGFNGIICPIIDALRGNVYASLYKKEGEELISLSEKECISLTDLIEKIKEKNEDVIF
                     VGDGISKHKEALKEALPNCSFAPSHSSYPRAASVGELGLRLLESGVSHDLNTYAPIYL
                     RKSQAEREYEERMRLK"
     gene            9971..10417
                     /gene="rimI"
                     /locus_tag="K8M77_001234"
     CDS             9971..10417
                     /gene="rimI"
                     /locus_tag="K8M77_001234"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBI7085739.1"
                     /translation="MNSYKIELMNSSHVKGVFEVSKLSLAESWNIDSIEKELSNKLAK
                     YLVALHGDKVIGFVGMWIVFHEGDITNVAVHPEYRKQGVGNLLIDNLITLCKENNINS
                     LTLEVRESNIPAQSLYKKHGFKEEGIRKNFYNNPKENAIIMWKHDI"
     gene            complement(10511..11107)
                     /locus_tag="K8M77_001235"
     CDS             complement(10511..11107)
                     /locus_tag="K8M77_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI7085740.1"
                     /translation="MKTKTLNTNRFIKLSLLSAIAVILMYIDFPVIPIFPWLKIDLSD
                     VPALMGAFAFGPLAGVIIELMKNLLILIIKGTGTGFVGEFANFLVGVALVWPAALVYK
                     KNKTKKTAILGMVLGFLCIEVVGILANVYLLLPAYGMAMSKAELMQYVTVGLIPFNGI
                     KSILVCGITYALYKKVSVSIFKVEPMLDKPKQMKENLG"
     regulatory      complement(11234..11351)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11455..13617)
                     /gene="tex"
                     /locus_tag="K8M77_001236"
     CDS             complement(11455..13617)
                     /gene="tex"
                     /locus_tag="K8M77_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein
                     Tex"
                     /protein_id="HBI7085741.1"
                     /translation="MDNINHILSKELGISLKQVISVIEMLDEGNTVPFIARYRKERTG
                     GLTDEVLRKFNERLTYLRNLESRKEDVLRIIEEQEKLTPELKLNIEKATTLTEVEDIY
                     RPFKAKKRTRATMAIEKGLKPLAELILSGEFNGDIVEEANKYINEEKGVKNEEEALQG
                     AMDIISEIISDNADYRKWIRNFVQKDGIIQVKGSSEEQTPYEMYYDYKEPVRTIPSHR
                     ILAINRGEKEKILSVKVTCNDDKIIDYLNKKVLKGNKITDKYLEESIKDSFKRLIYPS
                     IEREIRSELTSKGEEGAIDIFKANLKALLMQAPIKGKVVMGFDPGFRTGCKVAILDET
                     GKFVENTTVYPTAPQNRIDETISTLKKLIKKHGVQVISLGNGTASRESEEVIAKMLKE
                     IKDETGKELFYVIVSEAGASVYSASELANKEYPDLDVTVRGAISIGRRLQDPLAELVK
                     IDPKAIGVGQYQHDVTQKKLDESLAGIVEDCVNNVGVDLNIATPSLLSYISGINASIA
                     KNIVDYREENGKFKSRKELLKVKRLGQKAYEQCAGFLRVMESKEALDNTSVHPESYGV
                     AKELIKTLGYTEEDLKNGKLVDIDERVKAKGISNLAKELEVGEPTLNDIIKEIKKPGR
                     DPREELPKPIFKSGVIEMKDLKPGMILMGTVRNVSDFGAFVDIGVHQDGLVHKSQMAD
                     RFVKHPLDIVKVGDIVEVRILDVDLKRKRISLSMKKEG"
     gene            13729..15072
                     /locus_tag="K8M77_001237"
     CDS             13729..15072
                     /locus_tag="K8M77_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent exopeptidase M28"
                     /protein_id="HBI7085742.1"
                     /translation="MKKLYIATINILLCTFILLIQLSLVYKNRLDPFEREGVLKNIEY
                     LTSDELEGRLCGNEGNYLAQEFIENSFIKSNIKKLNSSYLQDFNVKAPILVEGEPYLK
                     VYDKNNKLVSSYKYGVDFKEAFINFKVNHITFDNTNEFKVLPTIMKGTSSSNNSVVFL
                     SSPVDSFNFRSSFIEDTPCDLYVVVAPNLIKELETYLNKGYKIDCFIPYEVKEKVVNN
                     VTGIIKGKNPFLPPLVLTAHFDHMGKGLSGEIYRGALDNASGASFLLELSSFLASLPQ
                     PSRDIIIVSLNAEEFGLLGSKNFAKENKDLLKNATVINFDMIGSDKDIPLTFMAGEDT
                     CFHSDLLDRLCEICNEKNITNIIENKDSSDHASFIKEGFDAITINDGDVTRIHTPEDK
                     IEYISPTAIDRSFSVVWSEIFNSAYSSSGLFLLDSKVLILLILSALVCLILKLRS"
     gene            complement(15323..16418)
                     /gene="prfB"
                     /locus_tag="K8M77_001238"
     CDS             complement(join(15323..16339,16341..16418))
                     /gene="prfB"
                     /locus_tag="K8M77_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592967.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBI7085743.1"
                     /translation="MIMELENQLSKLHELKNNLKEMGASLDLASLEKKSAELELKMQE
                     AGFWDDVQKAQEVTQEAKRVKDKIDKFKNLNERIDDVEVLKELMEENDEETAKEIISE
                     VKALSKEIDTLKIETILSGEYDRNNAILTLHTGVGGSDANDWTEMLLRMYTRWCEKKG
                     YSLETIDYLPGDEAGVKSVTLKVKGEFAYGYLKAEKGIHRLVRISPFNANGKRQTSFA
                     SVEVLPELTSDQDIEINPVDLRIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQN
                     ERSQFSNRDTAMGMLKSKLIELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYSMV
                     KDHRTNVETSNVNGVMGGDIDNFIIAYLNS"
BASE COUNT         5350 a         2838 c         1773 g         6615 t
ORIGIN      
        1 caattaactt atttttttat taaatttgat ttttctactt ttctcccatg ccattcataa
       61 tttctctaat ttgagtttta agtatatctg attttcctcc aaatatagct tgaatatttt
      121 ctccaacttg cattacacca gctgctccaa gctcttttaa tctttcttta ttaactttgc
      181 ttgaatcgtt aactgtaact cttaatctag ttatgcaagc atctaattta tttatattgc
      241 ctgcaccacc taaagctatt aaaatttcag cagctaagtc atcatttaca gtttggttat
      301 ccatatcatc aaatatgtca tcatcttctc tacctggagt tttaagatca aattttctaa
      361 taaagaatct aaataagaaa tagtaaatta caccaaagcc taatcctaca agtattacta
      421 accaccatct agttctattt ggaattactc ctaatagtat aaagtctaaa agtccacctg
      481 agaaagtcat acctatttta acatttaata attgcattat cataaaggat aatcctgcta
      541 atacgcagtg gattacaaat aatactgggg caacaaataa gaatgcaaac tctaaaggct
      601 cagtaattcc tgttaagaag gaagttaagg cagctgagaa taaaatacct gaagctattt
      661 tctttttacc tggtttagct tcatgataca ttgctaaggc tgctgctggt aatccaaaca
      721 tcataaacgg gaacttacca gtcataaagg tacctgctgt aaatggacct ccatctctta
      781 attgggcaaa aaatatttga ttatctccca ttattaattg acctgcttta tttgtgtatt
      841 ctccaaattg gaaccagaat ggattatacc aaatatgatg taatccaaat ggaattaatg
      901 ctctttctat tattccaaat aataaagctg atagggtttg attagcactt atcatatttt
      961 gtgagaagta caataaggca ccttgtattg gcggccatac aaataccatt attcctccaa
     1021 gcactaaggc agaaatagca gttattattg ggacaaatct tttacctgca aagaatccta
     1081 agtatgaagg taattctatc ttatagaacc ttttatataa agctgcggct ataactccca
     1141 taataattcc accaaatact ccagtttgaa gtgttggtat tccaagaact gtggcataca
     1201 taccattttt agtactagca accacgtcgg cagttattcc tccaccaatt cccattgtta
     1261 cattaagtat taaaaaacca actattgctg caagacctgc aacaccgtct ccttctgata
     1321 atcctacagc taccccaact gcgaataata atgctaagtt tgaaaatatt atattacctg
     1381 catcagacat tattgtagct attaattgaa accaccctgc atttaatgct ggcactaagc
     1441 ttaaaaactg aggatcttga aacattgatc caaatccaag taataaacct gccgctggta
     1501 ataaagcaac tggtaacatc aaagctttac ctattctttg taaaactcca aaagctttct
     1561 tcatcttaat ctctcctttt aaatgatttc tttctttata attcctaaaa tatataaaaa
     1621 ataaagactt aaatttatta ttcctataaa acacctattt tattattaat aggtattaga
     1681 ataataaatc taagtcttgc cttctaatta agtaacaatc ctaactattc tcttttattt
     1741 ttgtgatgat aatgcattat atatttttga aagattttct ttcatagcat ctatataact
     1801 tataccctct tcattacttt ctagagtata tattttttca atttttccac caacctcatt
     1861 agctaaagtt tgagatattt tttcacttgc taactcaggc ataaaaattg tttttatatc
     1921 atttttctta cagaattcaa ctatctcttg cattttttga ggagttattt ctccctctcc
     1981 aaataagttt tctacactaa cttgttttaa tccaaattct ctacataagt atccaaaagc
     2041 agcgtgacca gtaacaaaat ccttattagc taatccatta aatttttctc tgtactcatt
     2101 tgctaaagaa tctaatttct ctgcaaactt tttaaagttt tcttcgtaat aacttttatt
     2161 atctgaatca acctcaacta aagcatcttt tatgttttga gcttctattt tagctccatc
     2221 taaacttaac caaagatgag gatctacttt atcgccctct tttagtactt cagccccttt
     2281 agaagcttca accactttca catctttatt ttctattgta ttaagaactt gctctttcca
     2341 gtgttccata cctagtccat tataaacaaa aaccttcgcc ttatttaact ctaataaatc
     2401 ttgagttttt ggttcaaaat catgtggctc tgttccattt ggaaccatag tcttaacatt
     2461 taccttatct ccaccaacta tttccgtaaa ctctcttaaa ggttcaatac ttacagtaac
     2521 atcaacctta ccgttatcct ttttattttc tgctttctta gcactttgac acccaacaaa
     2581 aaaagcagcc attgttactc ctaaaaaaat cgatactaat ttctttctca tacttcctcc
     2641 agtttgcaaa tgatttgcat tattatatta taaactttat attacttttc tattattgtc
     2701 aatatttata ataaaactat cataaaaata aaaaagacct aatcaaaata tattataaat
     2761 aaaacttata aacatattcc tagaattact aggtatagct aataattgtt tgtaatttat
     2821 ataaaattta ttttgatata agcccttcat tttaaattaa agctctatta tatttacttt
     2881 ttccttatct ttgagaatat ttatttctct tttttggcat gtaaataaaa ttatttgatt
     2941 tttataaact ctcttatata ttatgtctaa catagttttt aatctattat catcatactg
     3001 agtaaaactt tcatctaata ctattggaac ttttttatct tcaaatatta tgtctattat
     3061 gccaagtcta agtgctaaat atatttggtc atatgttcca ctacttagat aagaagcatc
     3121 cataatttta ttgctttttg tatccttagc aactaagtta taatcttctg atactcttaa
     3181 atctttataa gcacccttag ttacatcttt aaagatatcc tctacctttt tatttataac
     3241 aggtccaaaa cttttttgaa gttccttaaa ggctttttcc atgtagtttg ttgcaatatt
     3301 aaccccctta agttttttct ctaaggactt aattctttcc ttaacttcgg ctatctcttc
     3361 atctattaaa aataaacttc tacaggttaa ctctttgcta ctaattaaat attctacatc
     3421 ctttatttct ttttcaatat ttaacaagtc attatttttt aattttattt catttaagat
     3481 ttcttcttca ctgttataaa cttcattttt aatctctgat tcctcatatg aaagtccatt
     3541 tttaacttct aaaatatctc ttcccttaag taatagattg caattttctt ctacactatt
     3601 taacttattt tttaagcctt ccttatattc tattttagct tctatttctt ctaatctaga
     3661 atatacttca tgtaaaggaa tactttgtag tgatgtattt tctaaataag cttttatttt
     3721 ttcttctatt tctaatcttc tattatttaa ttgatttaaa tcttccttaa tctcattaaa
     3781 ctctgatttt tttcttatca ataaatcttc ctggcttttc ttttcccttg aaagttctaa
     3841 gatttcatct atagaacctg cccctgtaac tcttagtaat ttatctataa aagcttcatt
     3901 acttaatatt ttttccttag ttttatcttc ttttaataaa atcaaacttt tctctttttc
     3961 cttaatttct acatagagaa tatctaactc ttttttaata aatctataat cctctagagt
     4021 ttctttaata aaatactcat cttttacctt aagaaattgt ttatattcat caattttact
     4081 ttttaatacc tctatttctt ttttctcatt tataagctca tctatttcat ttattctttt
     4141 acttatatta tttttttctt ttttatttaa gctactttta gctagtaaaa atataaataa
     4201 taacatacct aaaattgaaa gtgctaaggt tattatattt tcgctcttga aataggatat
     4261 aaataagaaa gcacataata aaatacttat tacttcccca actctaaaaa gaaaaatttt
     4321 tcttgaaaaa cttttatttt cctcttcaat aatattcttc tcctctttta agtttgcatt
     4381 tattgaagga tatatttttt cattattata tttctctaaa tccttttcta tttgtaacac
     4441 caaattatca tttaatttca aggcatttaa atgacttaaa aaatcatctt tcttttcatt
     4501 taactgataa agctcttcct ttaccttttg aataacattg taattttctc ttaatctatc
     4561 atcttcaatc ctaagctcta ttaatttagt ttctaaatta tttgatattt catttaaaat
     4621 tccataactc tttaattttt cattatactc ataaatagat tcttttaact tatctatttt
     4681 atcttccttt tcttttagtg attcttttag aaactcataa tttttaaact catctcttaa
     4741 gttatttaac tttttcaaat tacatatagc ttgtacatct tttaactctt ctaataaaac
     4801 ttttaattcc tcttctaatt tttctttttt atttaaatac tcatttatat tttcatactc
     4861 actttttaat tctgattttt ttaaatttct cttatgtaat tctaattctt ctatttttaa
     4921 atttaaatca gttctttttt cttttaatcc ctttaagtaa agctcattct ctatattttc
     4981 ctcatgaatt tttactcttt tatatctttc ttctaaaagc tcactttctt taattcttaa
     5041 taaatcaagt tttcctgttt ttcttgaatt agttaaggac tttttatttt cctctaaatt
     5101 attttttatt tttttataag aaacctcatc agctccacat tctaagatat taataatttt
     5161 atttaataca tcatcctgct tggaactctc tacagtacac cccatttgac ttataaatag
     5221 agtattctta aatccctcgc tagaaagatt aaatatatcc ttaccaggtt cttgccatga
     5281 gtatttattt atcttttctc cagttatatt atttaatata tttccatcat catatttttt
     5341 tgttttccca aaacttctat ctattaaaat ttcctcacca ttatcctcta caagtaactc
     5401 tcctgaaaaa cttccttccc ctaatggtgt gaactttgct ctatctgata aagttttttt
     5461 gcctctttta ttacaaacac catataacat tacctttatg aaagattgta atgtactttt
     5521 tccactttca ttttcaccat atattatgtt taatccatct tttaattcta tttctttatt
     5581 ttttaatttt ccaaaggact tcacatttat tttcttaatt atcatttaaa ttcacttctc
     5641 catctaaaat actctccata ccaattctaa aagctatatc tagtatctct tcatcctctt
     5701 cattagattc taaagcatcc ttgattaaag aacaaaaaat acccttaatt gaatactcat
     5761 tgctcatttc atcaatatct attttcattc tagtagaatt tacaatctta cagtaaaaga
     5821 atttatctct aattaagttt tctattaaat cactatctat tttaaaagac tcttctatat
     5881 tcccttttaa gatgatttta aatagatttt ttgctcttga ttcttttgaa atagtattaa
     5941 gtatcttatc tataacttct tctttagttt tacaatttga tatatcaatt tccttaagaa
     6001 aatactctct tttattagtc tttacaaaat ctaagtctaa agctccaagg gatacctctc
     6061 ctagtattac tcctttatct ccctcttcat caaaacccct tccctctgga catcctgaat
     6121 atgcataata ggttttaccg attcttttta ttcctgaaaa tttatgaata tgtcctaaag
     6181 caatataatc catcttactc tcttctattt ccttaaataa taagggatta tactctcctg
     6241 tttcattatt ccctaaatca ccatgtagaa ccattatttt tactaaatcc ttatcctctt
     6301 cttttggagt aaaatcctta agctttgact cttttatata tttttcctta aaggatgctc
     6361 cataaacaac agtatttaat tcaggtattt ctactctttc aatttcatcc ttaaatatat
     6421 gaacattttc ccctaagtta accatattat aaaaagattt tttattatat gggtcatgat
     6481 ttcctgctga aataaatact cttatattgt gttttttaag tttatccatt tgatccttta
     6541 taaaagaaag agtactcttt tctatagtat cattgtcaaa aaggtctccc gctaaaagaa
     6601 ctaaatcaac tttttcatca attcctctat ttattatttt actaaagctt tctcttaact
     6661 cagctctcct cattgtacca atttcctttg gtaactcttt aaaaggagta tcaaaatgaa
     6721 tatcactaca atgcaatatt tttacctttt tcaccaatat gacacctctc ttattccaat
     6781 ttattctaat tttatcatag agattgtcaa aaaaaaagac tacatagagg atataaatcc
     6841 tctatgtaat ctttttttgc ggtagaagtt taagcatcat gttttggcgg tttaccctcc
     6901 ataacacttt ctcctgttat aaatttttag agtcataata taggattttt ataatggtta
     6961 tttaataatc attataatta ttcctatttt ttaatgaatc ttttttcttt aattcagtat
     7021 tagcgaaagg tttgttattt tatttgactt tcgtaagctt ctaccattct ttttaccatt
     7081 tcaccgccta cggaaccaca ttgtcttgaa cttaggtctc cattgtaatc tgagaatggt
     7141 acacctaatt ctctagctac ctcgtttttg aatttactta atccattttt tgcttctgga
     7201 actaatgatt ttgacatttc taattcctcc tttggctttg gtaatttgta agcttgaaat
     7261 ttgtttatct cttgcttaca ctattatatt aaccataatt cagattataa acctatgaaa
     7321 tagaaggatt attaaccatg ttattccaga aaaaatttat ctttatatac aatttacatg
     7381 ttttcagaat ataatctttt atttaaaatt aagcaaaaaa aatcagtgaa ttaattcact
     7441 gattttttgg caggggcagt aggacttgaa cccacaacca atggttttgg agaccactac
     7501 tctaccaatt gagccatacc cctataatat tatgttattt attgtgttaa ttggcagggg
     7561 cagtaggact cgaacccaca accaatggtt ttggagacca ctactctacc aattgagcca
     7621 tacccctgaa cacagaattt attatatatt aaaaataaac ttaacgcaag agttttttat
     7681 gtaatttttt atttttaata aaaaatctat attaatcccc taataaaata atataatatt
     7741 tgataataaa aaaatatatt tagctaaatt taaagtaatt atttcatcat ttcactctta
     7801 tcttcatcaa ttaaaataaa gcatatataa tataaattta aaaaatcaat attatatatg
     7861 ctttatactt atggtttata atagtttgct atgctaattt aaatcctgac tcttttaatt
     7921 tctttttaat attttcaaca tctatgcttt taaccctaac tataacctct ttaacatcag
     7981 ttaaactctt tgtatctaca ataactatgc ttaatatatc tccaccttct tcagatattg
     8041 tcttagttat ttttgataat tgtcctggga catcataaga tcttattact attctttccc
     8101 ctttattaag tccaaaagca ttattaaatt ctctaaaaac tgccttatga gttaatattc
     8161 cttgaaaatt atcaaattca tctacaacag ccacaaaaga aattctcatt ctttctagca
     8221 tggatactgc cttttctata tgttctgatt tatttattat aggaacttct gtttttaata
     8281 tttcatctac agtgatatta tttaatactt catctttact taaattattt ttaaagtaat
     8341 atttatagat agaactttta gctactgccc ctctaaactt atctccatct actacaggaa
     8401 ttgataaaaa cttattatcc tccataattt ttagtgcatc tttaagttta gtattagaat
     8461 caactaaatc tagattttct ttctttgtca taatagcttt tactaacata caaatccccc
     8521 caattcctaa atttacatac tatatactat tattatacta ttatattatt acatagttat
     8581 ccaattatat taaatttatc aaagtattaa ttaaagatac ttttcccctt ttattgttaa
     8641 tattataata attttatagg tatatgtttt attaaatatt aggagaattt ttaattattt
     8701 aagaaacata ataaaacttt aaatacctta ttaaatctac tatctctttt ctttttcttt
     8761 aaatttggat agaatgatta aagagtgtta atttgaaagg agttttttat ggaatttata
     8821 gtagatagtg tagataaaac tatgcatatt ggaagacaac ttggttctct tgtaaataaa
     8881 ggagatattc tttgtttaac tggagactta ggaactggta aaactcatat aagtaaagga
     8941 attgcagaag gattaggaat tgacgagcat ataactagtc caacttttaa tatagttaat
     9001 gaatatcata gcggaagatt aactctttat cattttgatg tttatagagt taatgatcca
     9061 gatgaaatag aagcaattgg ctttgatgaa tatatctttg gtgatggagt aagcttaatt
     9121 gaatgggcta attacataga agaacttatt cctgaagagt atatacatat aaaaatagaa
     9181 aaattacctg atatgggtga aaattttaga aaaataagta ttaatggtta tggtgataga
     9241 tataactaca taaaggagat agaagaatgt tagtattagc tattgattca tcttctagca
     9301 cagctacttg cgcacttgtt agtgaaaagg gagttctagg agaaataaac ttaaatgata
     9361 aaaaacaaca ttccgtttta ttaatggact taatagatga tttacttaag tataataatc
     9421 taaagattaa agatttagac ggatttgcta ttagtgaagg tcctggctca tttactggac
     9481 ttagaattgg tatggcaact gttaaaggat taagctttgg aagtaaaaaa ccttctgttt
     9541 gtatttcttc ccttgatgct ttagcttata atatttcagg atttaatgga ataatctgtc
     9601 ctattataga tgctcttaga ggaaatgtat atgcttctct atataaaaaa gaaggagagg
     9661 agctaatatc tcttagtgaa aaagaatgca tttctttaac tgacttaatt gaaaaaataa
     9721 aagaaaaaaa tgaagatgtt atttttgtgg gagatggaat aagcaaacac aaggaagctt
     9781 taaaagaagc tcttcctaat tgctcttttg cacctagtca ttcatcttat ccaagagcag
     9841 catcagttgg agaattagga ttaagattat tagagtcagg agtttctcat gatttaaata
     9901 catatgctcc tatatactta agaaaatctc aagctgaaag agaatatgaa gaaagaatga
     9961 gattaaaata atgaattcat ataaaataga acttatgaat tcttcccatg ttaagggcgt
    10021 ttttgaagta agtaaactta gtcttgcaga atcttggaat atagattcta tagaaaaaga
    10081 actttctaat aaactagcaa aatatcttgt tgctttacat ggagacaagg ttattggctt
    10141 tgttggaatg tggattgttt ttcatgaagg agatataact aacgtagcag tacatcctga
    10201 atatagaaaa caaggagttg ggaatctttt aatagataat ctaattactt tatgtaaaga
    10261 aaataatata aattctctta ccttagaagt tagagagtct aatattcctg ctcaatccct
    10321 ttataaaaag catggattta aggaagaagg gataagaaaa aacttctaca ataatccaaa
    10381 agaaaatgcc ataataatgt ggaaacatga tatttagaat atatttttaa ttagtattta
    10441 tttttatcta aatctttaac actaatataa aataataggg actatatcat tttgatatag
    10501 tccctttcta ttatcctaaa ttttctttca tttgttttgg tttatctagc ataggttcaa
    10561 ccttaaatat tgatacagaa acctttttat ataaagcata tgttattcca caaaccaata
    10621 tagattttat tccgttaaat ggaattaatc caactgttac atattgcatt aactctgctt
    10681 ttgacatagc cataccgtat gctggaagta ataaataaac attagctaat attcctacca
    10741 cttctataca taaaaatcct agtaccattc ctaaaatagc agttttctta gttttatttt
    10801 tcttataaac taatgctgct ggccatacta aagctactcc aactaagaaa ttagcaaact
    10861 cacctacaaa tcctgttcct gtccccttta ttattaatat taataaattt ttcataagtt
    10921 ctattattac ccctgctaat ggaccaaaag caaaggctcc cataagggca ggcacatcgc
    10981 ttaaatcaat cttaagccat gggaatatag gtattactgg aaagtcaata tacataagta
    11041 ttactgcaat tgcagataaa agtgataatt taatgaatct gtttgtgttt aaggtttttg
    11101 ttttcatttt ttcccctccc aaaagttact tcctctgggg ctattaagtc tttaatgatt
    11161 tttttcaaat aaaaaagccc tgatacatat acttatatca gggcatatag attcagaatt
    11221 taaatcatta aacactcatc ttctttcatc cagactatac tgtcggcctc ggaatttcac
    11281 cgaatcatgc taaaaactag ctcgcgggct ttaccgccgg tggggacttg caccccgccc
    11341 tgaagacttt ttttatttca tttgttataa ttttaactct tttttatata aatttcaata
    11401 tattaaatat ttttatctac taaaattttc atttatttta attaattttc taacttaacc
    11461 ttcttttttc attgataatg aaattctctt tctttttaaa tcaacatcta atattctaac
    11521 ttctactata tctccaacct taactatatc aagtggatgt ttaacaaatc tatctgccat
    11581 ttggctctta tgaactaatc catcttggtg aactccaata tctacaaaag caccaaaatc
    11641 agatacattt ctaactgtcc ccattaaaat catacctggt tttaaatctt tcatttctat
    11701 tacgccagac ttaaatattg gtttaggtaa ttcctctctt ggatctcttc caggcttttt
    11761 aatttccttt ataatatcat taagtgttgg ttctccaact tctaattctt ttgctaagtt
    11821 agaaattcct tttgctttta ctctctcatc tatatctact aatttgccat tttttaaatc
    11881 ttcttctgta tatcctaaag tttttataag ttccttagct accccatatg actctggatg
    11941 aactgaggta ttatctaaag cttctttact ttccataact cttaagaatc ctgcacattg
    12001 ttcataagct ttttgtccta atcttttaac ttttaaaagt tcttttctac ttttaaactt
    12061 accattttct tctctataat caacaatatt tttagctatt gaagcattta tacctgaaat
    12121 ataacttaat agtgatggag ttgctatatt taaatctact cctacattat taacacaatc
    12181 ctcaactatc cctgctaagg attcatcaag ttttttctga gttacatcat gttgatattg
    12241 tcctactcct atagccttag gatctatttt aacaagctca gctaatggat cttgaagtct
    12301 tcttcctata gaaattgcgc ctcttacagt tacatctaag tctggatatt ccttatttgc
    12361 aagttctgat gctgaataaa cagaagctcc tgcctcagaa actataacat agaataactc
    12421 ttttcctgtt tcatctttta tttccttaag catttttgca attacttctt ctgattctct
    12481 tgaagctgtt ccatttccta aagaaataac ttgaactcca tgttttttaa taagtttttt
    12541 aagtgtactt attgtttcat caattctatt ttgaggggct gttggataaa ctgttgtatt
    12601 ctcaacaaat tttcctgttt cgtctaagat tgcaacctta catccagttc taaatccagg
    12661 gtcaaatccc attacaactt tccccttaat aggcgcttgc attaaaagag cttttaaatt
    12721 agccttaaat atatcaatgg ctccctcttc tcccttagag gttaactcac ttcttatttc
    12781 cctttctatt gaaggatata taagtctttt aaaggagtcc tttatacttt cttctaaata
    12841 cttatcagta attttatttc cctttaaaac ttttttattt aaataatcta taattttatc
    12901 atcattacaa gttactttaa cagaaagaat tttttccttt tctcctctat ttatagctaa
    12961 tattctatga gatggaattg ttctaacagg ttccttataa tcataataca tttcataagg
    13021 tgtttgttct tcactgcttc cttttacctg aattattcca tccttttgaa caaaatttct
    13081 aatccatttt ctataatcag cattatcaga tattatttca cttataatat ccatagcccc
    13141 ttgaagagct tcctcttcat ttttaacacc tttttcttca ttaatatatt tattagcctc
    13201 ttctactata tcaccattaa attctcctga tagaataagc tcagctagtg gctttaatcc
    13261 cttttcaata gccatagtag ctcttgttct ctttttagct ttaaaaggtc tatatatatc
    13321 ttcaacctca gtaagagtag tagctttctc aatattcagt tttaattctg gagttaattt
    13381 ttcttgttcc tctattattc ttaaaacatc ttcttttcta ctttctaaat ttcttagata
    13441 tgttaatctc tcattaaatt ttcttagaac ctcatctgtt aatccaccag ttctttcttt
    13501 tctatatcta gctatgaaag gtactgtatt tccttcatct aacatttcta taacacttat
    13561 tacctgtttt aatgaaatac caagttcctt tgataaaata tgatttatat tgtccataat
    13621 atcctcctat gttttatcta ttaatttaat attaatagta attttttgaa taaacataat
    13681 ttaaatctca tacttttaat tataacctaa ttatttatgg aggataatat gaaaaaatta
    13741 tatatagcca caataaatat acttctttgt acatttattt tgcttataca actctctcta
    13801 gtttataaaa ataggcttga tccctttgaa agagaaggtg ttttaaagaa tatagaatac
    13861 ttaacctctg atgaattaga aggaagactt tgcggaaatg aaggtaatta cttagctcaa
    13921 gaattcattg aaaattcttt tattaaatca aacataaaaa aacttaattc tagttatctt
    13981 caagatttca atgtaaaagc tcctatttta gttgaaggag agccttattt aaaagtttac
    14041 gataaaaata ataaattagt atcttcatat aaatatggag tagattttaa agaagccttt
    14101 ataaacttta aggtaaatca tataactttt gataatacaa atgagtttaa agttcttcct
    14161 acaataatga aaggaacttc ttcatctaat aacagtgttg tatttctctc atctccagta
    14221 gattctttta attttagaag ctcttttatt gaggatacac cttgtgactt atatgtggta
    14281 gtagctccaa accttataaa agaactagag acatatttaa ataaaggtta caaaatagac
    14341 tgttttatcc cttatgaggt taaagagaaa gttgttaata atgttactgg aattataaaa
    14401 ggtaaaaatc catttttacc acctttagtc ttaacagctc attttgatca tatgggaaaa
    14461 ggactttctg gtgaaattta tagaggtgct ttagataatg cttctggtgc ttcattttta
    14521 ttagaattaa gttctttcct agcttctctt cctcaaccat caagagatat aataatagtc
    14581 tctctaaatg ctgaagaatt tggattacta ggttctaaaa attttgctaa ggaaaataaa
    14641 gacttattaa aaaatgctac agttattaac tttgatatga ttggaagtga taaagatatc
    14701 ccactcacat ttatggcagg agaagatact tgttttcaca gtgatttact agataggctt
    14761 tgtgaaattt gtaatgaaaa gaacataact aatattatag aaaataaaga ttcctctgac
    14821 cacgcaagct ttattaaaga aggttttgat gcaataacaa taaatgatgg tgatgtaaca
    14881 agaattcaca caccagaaga taaaattgaa tatataagtc ctacagctat agatagaagt
    14941 ttctctgtgg tgtggagcga aatatttaac tcagcttact catcctctgg attattctta
    15001 ttagactcaa aagttcttat attactaata ttatcagctt tggtttgttt aatattaaag
    15061 ttaaggagtt aaaatatata atttaaaaat tcttatacaa aaaacagtag ataattttgt
    15121 ttttttatac aaaactatct actgtacctt taaatagaaa ttagcattta atatcttata
    15181 tagaaaactt aatctaaaaa actaaatact aggctagcta cttaatagtt tttaagatgt
    15241 tttcaaagta attacttaaa attcttagtt taattaatta attctcataa cctatttata
    15301 aattttttat atagtcacct ttttatgaat ttaaataggc aattataaaa ttatctatat
    15361 ctccacccat tactccgtta acattagatg tctcaacatt agttctatga tccttaacca
    15421 ttgaatatgg atggaaaaca taacttctta tctgacttcc ccatcccata tcttttaatt
    15481 cacctgttaa atcctctatt ttttccttat gagctctttc tttaagttct ataagtttag
    15541 actttaacat tcccatggca gtatctctat tagaaaactg gcttctctca ttttggcatt
    15601 gaactactat tccagttgga atatgagtaa ttcttactgc tgactcagtt ttatttacat
    15661 gctgaccacc agctccacct gctctataag tgtctattct taaatctaca ggatttattt
    15721 ctatatcttg atctgatgtt aactctggta aaacttctac tgaagcaaag gaagtttgtc
    15781 ttttaccatt ggcattaaaa ggtgatattc taactaatct gtgaataccc ttttcagctt
    15841 ttaaatatcc ataagcaaat tcacctttaa cttttaaagt aacactttta actccagctt
    15901 catctccagg taaataatca atagtttcta aggaatatcc cttcttttca caccatcttg
    15961 tgtacattct aagaagcatt tctgtccaat cattagcatc gcttccccca acacctgtat
    16021 gaagtgttaa tatggcatta tttctatcat attccccaga aagaattgtc tctattttta
    16081 atgtatctat ctctttactt aaagccttaa cttcgcttat tatctcttta gctgtttctt
    16141 cgtcattttc ttccataagc tcttttaata cttctacatc atctattctt tcatttaaat
    16201 ttttaaactt atcaatttta tcttttactc tttttgcttc ttgggttact tcttgggcct
    16261 tttggacatc atcccaaaat cccgcttctt gcatcttaag ctctaattct gcactcttct
    16321 tctctaaaga tgctaagtca aagtgaagcc cccatttcct ttaaattatt ttttagttca
    16381 tgtaactttg ataattgatt ttcaagttcc ataatcataa aaacttcacc cctagcttta
    16441 attattttga tattataaag tatatcattt tatagctatt taaaacaata aaatcatatt
    16501 tagtaaaaat ctaaatataa tattaaattt aataaaaata ttttaaaaaa tttaaatatc
    16561 agtagaagta caaatt
//
LOCUS       DAEVGI010000045        15966 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEVGI010000045 DAEVGI010000000
VERSION     DAEVGI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 15966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15966
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.045"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            37..717
                     /locus_tag="K8M77_001239"
     CDS             37..717
                     /locus_tag="K8M77_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBI7085744.1"
                     /translation="MELIGIVIVIIGFALKFDTIAVVLVAGLSTGLVAHMSLYEILEK
                     LGSTFVSQRHMTLFILTLPVIGMCERFGLKERAITLIKNMKNMSTGKLLSCYLFIREV
                     GSAVSLRLSGQAQFIRPLINPMAQGAAISKYGKLDDKNEDLIKGTAAAMDNYGNFFGQ
                     NVFLASSGVLLIAGTLEEIGYGVNALDIAKASVPIAIIALILGVLQNRLLDKRLSRNL
                     SKNKEEIK"
     gene            730..1659
                     /locus_tag="K8M77_001240"
     CDS             730..1659
                     /locus_tag="K8M77_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBI7085745.1"
                     /translation="MNIQMISNVLLEIFYIIVGLLMLNTAIDTLKDQEHKTRIGTAIF
                     WTILSIIFIAGGAIPPVVVGALLLVIGVLTASKQVNIGNLKMPNVDFAEIQAEKLKNK
                     IFLPSIVIAVGSLAIAQFTSLSGTVAIGIASVAAVITTFLVLKAKPKHLVEDSNRMVQ
                     SVGSTSILPQLLAALGTVFTAAGVGDVISSGISNFIPEGNILAGVIAYCVGMAVFTMI
                     MGNAFAAFSVITVGIGLPFVFAQGANVAIAGALALTAGYCGTLLTPMAANFNVMPAAL
                     LETKDKNVVMKCQSLFAIILLVIHIALMYFLAF"
     gene            1695..2336
                     /gene="pcp"
                     /locus_tag="K8M77_001241"
     CDS             1695..2336
                     /gene="pcp"
                     /locus_tag="K8M77_001241"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBI7085746.1"
                     /translation="MKVLITGFDPFGGESINPALEAVKMIPENIEGAQVIKLEIPTVF
                     RKSLEKIEKKIEEINPDIVISIGQAGGRFGVTPERVAINMDDARIEDNEGNQPIDISI
                     YEDGESAYFSNLPIKAMVKEMVDNGIPASVSNTAGTFVCNHVMYGVLYLVNKKYKNIR
                     AGFIHVPYIPAQVVNKPNTPSMSINDIAKGLELSIKAIVLNDNDIKTVGGAVC"
     gene            2336..2776
                     /locus_tag="K8M77_001242"
     CDS             2336..2776
                     /locus_tag="K8M77_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBI7085747.1"
                     /translation="MLEPKVKRKIFVYGSLRTGFFNYEKYLKGKVIKSELGRVKGTLY
                     HMPKKGYPALIEGDGFVYGEVMTIIDYENVINAIDLMEGYLGVNKKNNEYNRIEMDVE
                     ILGKKSSEKCYVYYYGMNDKDDFENNSILIQDGNWRNFMLKKVS"
     gene            complement(2822..3004)
                     /locus_tag="K8M77_001243"
     CDS             complement(2822..3004)
                     /locus_tag="K8M77_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBI7085748.1"
                     /translation="MSQHLVPEAKNGLSKFKNEVANEMGVPFSDYNGDLSSRQCGSVG
                     GEMVKRMVEKYEQSMK"
     gene            complement(3244..4221)
                     /locus_tag="K8M77_001244"
     CDS             complement(3244..4221)
                     /locus_tag="K8M77_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBI7085749.1"
                     /translation="MFEIKITDIDGFKLGHAQDFEGATGCTVLLCEEGASGGVDVRGG
                     APGTRETDLLNPMEMVDKVHAVVLSGGSAFGLDSCSGVMEYLENKNVGFDVGVTKVPI
                     VCGAVLFDLACGNPKVRPNKKMGLEACKNSETYFDSKNGNIGCGTGATVGKALNQKLA
                     MKGGFGSYAVQIGDLKVGAIVGVNSLGDIVDPNDNNKIIAGGLSQDRNSFMNIEKSLL
                     ANYSNPKNVFKGNTTIGCIVTNGNFNKAEANKIASMAQNGFGRTIRPAHTMFDGDTIF
                     TLSSNKVKADINVVGLLAAQVMEKAIIKAVKEADSSYGFLSHKDLKFNV"
     gene            complement(4424..5176)
                     /locus_tag="K8M77_001245"
     CDS             complement(4424..5176)
                     /locus_tag="K8M77_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI7085750.1"
                     /translation="MNAVYQTRQKTKKLVVIGMLSGISIFLGLTGLGFINIPPVKATI
                     MHIPVIIGAIIEGPIVGGLIGLVFGLFSMYQAFTASTPTSFMFWNPIIALVPRILIGV
                     ISYYVYVSMIKGSKKNLKATSIISSLILGVLLFVSSEALTDSLTISAVIGVLFFLASM
                     IFMFKFKHKNKEEALSIGTASVIGTLTNTIGVLGLTYVFYLERYASALGISEHAAKLT
                     LAGIGVTNGIPEAFISALISIPVILGVRKILR"
     regulatory      5664..5761
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5829..7217
                     /locus_tag="K8M77_001246"
     CDS             5829..7217
                     /locus_tag="K8M77_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBI7085751.1"
                     /translation="MENLLNQIDNLVWGVPLLMLLVGTGIYLTIRLKLLQILKLPLAL
                     KYVFKKDDESTCEDAEGDVSSFGALCTALSATIGTGNIVGVATAIKAGGPGALFWMWV
                     AAFFGMATKYAEGVLAIKYRIVDENGQMAGGPMYYIKNGLGLNWLANIFAFFGICVAL
                     LGIGTFGQVKSITDAASITFNVPTIITAGVVTLLVALVILGGIKRISSVSEMVVPLMA
                     GLYILGVLIVIAFNLDKVPHAVSIIIESAFNPKAALGGAVGVSIITVMKSGIARGVFS
                     NEAGLGSAPIAAAAAKTKSPVKQGLISMTGTFFDTILICTMTGIVIILTGAYSGSLEG
                     AALTTQAFEIGLPISNIGTYIVNIGLMFFAFTTILGWNYYGERCIEYLFGIKAIKPYR
                     ILYIILVAIGSFLPLTLIFIIADIVNGLMAIPNLVGIIGLRKVVIEETEEFFREKALS
                     EESAELEGTALN"
     gene            complement(7261..7989)
                     /locus_tag="K8M77_001247"
     CDS             complement(7261..7989)
                     /locus_tag="K8M77_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBI7085752.1"
                     /translation="MFRSIYWYGKGYAWTLIKSLSDIKKINKMEEEGKLEERDEYIYQ
                     CSRRIAHTFLKDAGIEVNVEGLENIPDGPVLFVCNHQSNMDIPVIMYSIDRPKGFIAK
                     KELEKMPILKDWIILSKAIFIDREHPKKAMAGIIQGIKQLKEGYSMVVFPEGTRSQCS
                     KMGEFKAGSFKLATKPKVPIVPLTIDGTYKIMEGNHYFIKPGKVNLYIHPPVYTDKLT
                     KEEITELPEKVENIVRSKLPNKGR"
     gene            8231..9070
                     /locus_tag="K8M77_001248"
     CDS             8231..9070
                     /locus_tag="K8M77_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBI7085753.1"
                     /translation="MKNLDLKNGVIIADGAMGTRIMELGVNLKETPSELLNIKKPELI
                     EKIHREYIESGANLILSNTFMCNIINAKRNNYNLEEVIEGGISSAKKACGDHGLVALD
                     IGPLSYYIEENDSSFKEIVYENTERIINASKDKFDLVIFETLGSLKEGEFAVKKAKTL
                     TDKKVICSFTLAYKKDIPNFIKNMVSTLEPLGVDALGINCTGYEEILMALDILKENTN
                     LPIMIKANLGIPKKVGEEFIYDKTLEEFKNLSKRALEKGVNIIGGCCGTTPEYIRTIC
                     NLK"
     gene            9307..11886
                     /locus_tag="K8M77_001249"
     CDS             9307..11886
                     /locus_tag="K8M77_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBI7085754.1"
                     /translation="MKKVHVVSHSHWDREWYIPFQKHRVKLVDFMDSLINTLENNEHF
                     KYFHLDGQTIALDDYLEIKPYMFHRLKKLIEEGRLHVGPWYVLQDEYLISAEANVRNM
                     LLGMRFAKRFGNPVKIGYFPDSFGNISQAPQILRGFGIDNAVFGRGITPIAFDNQKVE
                     EAGENEYHSEVLWKSPDGSEVLGILMANWYSNGNEIPAGKKEAIEFLNDRVNKADMYA
                     TTNQILLMNGCDHQPVQTNVGDIIDNVKEDVDFELVHSNLVQYIEDVKKEVKDLKEVT
                     GELNGQYTDGWWTLCNTASSRLYLKQMNNKSQVLLERYVEPLGVMASLEGKKYESDFI
                     LQAWKYLIQNHPHDSICGCSIDEVHQDMVTRFKSSMQISEEMIARSTEHMLNLVNGTT
                     TDRESIVVFNTLGWDRSEVVEATVDVNKESGIENFKVVTENGEEVPSVVEDLGETFKY
                     TLPNNRFRQPEYVNRFKVKFLANNIKALGLKSFKIVSCEKREEKEVSKDLTFENKFFS
                     LKFNENGTFDLIHKGTNKEYKNLNAFEDCGDIGDEYIFRAPKKDLVVNTLGNKASINI
                     LKDNEVEKVIEVKNTLEVPVKAIKKESRRVEEMTEIKLVSTIILSEISKRVDVKVEIE
                     NTAKDHRLRALFPTGIEASTHFTEGHFDVIERETTPWEGWQNPSNCQKQTNFVDVNDG
                     KVGLMIANMGLPEYEVLRDGKNTIALTILRSVGELGDWGVFKTPEAQCLGNNVAEYSI
                     IAHEGDYAGERAYEEAYNLNTPMLALQGDFKKEDSLVSIKGENVVLSAFKKAEEGDSI
                     IIRIFNISHKEENFQIKVNRDVKEINEVNLNEELIEKLEVKENAINGIISKKQIKTFE
                     IVI"
     gene            12261..12698
                     /locus_tag="K8M77_001250"
     CDS             12261..12698
                     /locus_tag="K8M77_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBI7085755.1"
                     /translation="MEGNLLVIDKRVLPEVFEKVINAKRLLKEGKVKEITEATKQAGI
                     SRSVYYKYKDYIFEFAETLQGRKVIFNMVVTHEKGVLSSVLNILSDVGGNILTIDQGL
                     PIHGLAHVSFTIDISTMKCDIKEMLNEIELVHGVEKVEFVAME"
     gene            12871..14016
                     /locus_tag="K8M77_001251"
     CDS             12871..14016
                     /locus_tag="K8M77_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBI7085756.1"
                     /translation="MEVYFDNSSTTKSLKEVRDEVYYAMDEFWGNPSSLHKLGVKMQR
                     KIEELQERIAKKINASKEEIIFTSGGSESNNMIIKGLARENNHIITTTFEHSSVLNTY
                     RELEKQGVSVTYLKVNNKGFIDLKELEEAINKNTILVSIMQINNEVGSIQKIKEIGRL
                     IKEKSKRAKFHVDGVQGFGKFEIDVKACNIDFYSVSAHKFHGPKGVGFMYMRKGLNLK
                     SLITGGEQQRGLRAGTENTPSYMGMVKAMDIAYDALEDSYNHVKNLKEYFIEKLSKIE
                     NVVINSPSSEEYSPYILNVSFLGTRSEVLLHILEEDNIFVSTGSACSSKASVAKGSYV
                     LNAMGLEPKCIQGAIRFSFSRYNTLEEVDYTIASLEKALKFLRRIKI"
     gene            14013..15170
                     /gene="thiI"
                     /locus_tag="K8M77_001252"
     CDS             14013..15170
                     /gene="thiI"
                     /locus_tag="K8M77_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBI7085757.1"
                     /translation="MNNLILVKYASEIFLKGLNKNKFERKLKENIRKKLKDIDHEFIT
                     DQNRWFIKSEDLDGVIERVKKVFGVKELCLVTQVTGDFNSIKEEGLKKIKESKAKSFK
                     VETNRANKKFPMNSMEVSRAVGGYILSELGDEIEVDIHNPECKLYVEIRGNAYVFTDK
                     DKIKAVGGLPYGMNGSTMVMLSGGIDSPVAAYLMARRGVETHCVYYHSHPYTSERAKD
                     KVKELAKIVGRYTEKITLYVVPFTEIQMDIIEKCREDELTIIMRRFMMRVACELSERK
                     KIQSITTGESIGQVASQTMEGLMVSNDVSDRPVFRPLIAMDKEDIMDIARDIDTYDTS
                     ILPYEDCCTIFVPKHPKTKPRVKDMIIAERKLDIEALVNKAIDEMETFIFE"
     gene            15299..15556
                     /locus_tag="K8M77_001253"
     CDS             15299..15556
                     /locus_tag="K8M77_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBI7085758.1"
                     /translation="MERNIEKSIGKIFRHFKGDLYLVEGVVTHSESGEKMVLYRALYG
                     ECGQFVRPYDMFLEEVPKEKENPTGQKYRFQEFEVKSLNRK"
BASE COUNT         6108 a         1829 c         2804 g         5225 t
ORIGIN      
        1 aaaatgaaat ttataactta aaaaggagag ataaaagtgg aactgatagg aatagtaata
       61 gtaataatag gatttgcact taaatttgat actatagcag ttgtattagt agcaggatta
      121 tcaactggat tagtagcaca tatgagttta tatgaaatct tagaaaaatt aggatcaacc
      181 tttgtaagcc aaagacacat gacattattt atattaactt tacctgtaat aggtatgtgt
      241 gaaagatttg gcttaaaaga gagagctata accttaataa agaatatgaa aaatatgtca
      301 actggaaaac ttttaagttg ctatttattt atcagagaag ttggatctgc agtttcacta
      361 agattaagtg gacaagcaca atttataaga ccacttataa acccaatggc tcaaggagca
      421 gcaatcagca aatatgggaa attagatgat aaaaatgaag atttaataaa aggaacagca
      481 gctgctatgg ataattatgg aaacttcttt gggcaaaatg tatttttagc aagttcagga
      541 gttcttttaa tagcaggaac tttagaagaa ataggttatg gagttaatgc tttagatata
      601 gcaaaagctt cggttccaat agccataata gctttaatat taggagtgtt acaaaataga
      661 ttattagata aaaggttaag tagaaaccta tcaaagaaca aagaagagat aaaatagggg
      721 tgataattaa tgaatattca gatgatttct aatgtattgc ttgaaatctt ttatataata
      781 gtaggacttt taatgttaaa tactgcgatt gacacattaa aagaccagga acataaaact
      841 agaataggaa cggcaatatt ttggactata ttgtcaatta tatttatagc gggaggagct
      901 attccaccag tggtagtagg agctttactt ttagttatag gagtattaac tgctagcaaa
      961 caagtaaata taggaaacct aaaaatgcct aatgtagatt ttgcagaaat tcaagctgaa
     1021 aagttaaaaa ataagatttt tttaccttca attgtaatag cagttggatc tttagctatt
     1081 gcacaattta caagtttatc aggaactgtt gccataggta tagcttctgt ggcagctgta
     1141 ataacaactt tcttagtatt aaaggctaaa ccaaagcatt tagtagagga tagtaacaga
     1201 atggttcaat cagttggatc tacaagtata ttacctcaac ttttagcagc cttaggaaca
     1261 gtgtttactg cagcgggagt aggagatgtt atatcatcag gtatatctaa ttttattcct
     1321 gaaggaaaca tattagcggg tgtaatagct tactgtgtag gaatggcagt attcactatg
     1381 ataatgggaa atgcctttgc agcattctca gttataactg tgggaatagg attaccattt
     1441 gtatttgctc aaggagctaa tgtagccata gcaggagctt tagctctaac tgcaggatat
     1501 tgtggaacat tattaacccc aatggcagca aactttaacg ttatgccagc tgctttatta
     1561 gagacaaaag ataagaatgt ggttatgaaa tgtcaaagtt tatttgctat aattttatta
     1621 gttatacata tagctttaat gtatttctta gcattttaat taattaggta ttataaagta
     1681 aggagtgtat gagaatgaaa gtattaatta caggatttga tccctttgga ggagaaagta
     1741 taaacccagc tttagaagct gttaaaatga taccagaaaa tatagaagga gctcaggtta
     1801 ttaaattaga aatacctaca gtttttagaa agtctcttga aaaaatagaa aaaaaaatag
     1861 aggaaattaa tccagatata gttatttcta taggacaagc tggaggaaga tttggtgtaa
     1921 ctccagaaag agtagctata aatatggatg atgcgagaat agaggataat gaagggaatc
     1981 aaccaataga tatttctata tatgaagatg gagaaagtgc atactttagt aatttaccta
     2041 taaaagcaat ggttaaggaa atggttgata atggaatacc tgcatcagtt tctaacacag
     2101 ctggaacttt tgtttgtaac catgttatgt atggagtttt atacttagta aataaaaagt
     2161 ataaaaatat aagagctgga tttatacatg taccttatat tccagcacaa gttgtaaata
     2221 aacctaatac accttctatg tctattaatg atatagcaaa aggattagag cttagtataa
     2281 aggctatagt tttaaatgat aatgatatta aaactgtagg aggagctgtt tgttaatgtt
     2341 agaacctaag gttaaaagaa aaatctttgt ttatggaagt ttgaggacag gatttttcaa
     2401 ttatgaaaaa tacttaaaag ggaaagttat aaaaagtgaa ttaggaaggg taaagggaac
     2461 tttatatcat atgcctaaaa aaggatatcc tgctttaatt gaaggggatg gatttgtata
     2521 tggagaagtt atgactatta ttgattacga aaatgttata aatgctatag atcttatgga
     2581 aggatactta ggagttaata agaaaaataa tgaatataat agaattgaga tggatgttga
     2641 aatcttagga aagaaatctt ctgaaaaatg ctatgtttat tattatggaa tgaatgataa
     2701 agatgacttt gaaaataaca gtatattaat acaagatgga aattggagaa actttatgct
     2761 taaaaaagtt tcttaaatta aaaataagag gtaagaattt tcttacctct taattttgtt
     2821 attatttcat tgattgttcg tatttttcta ccattctttt aaccatttct ccaccaacac
     2881 ttccgcattg tcttgagcta aggtctccat tataatctga gaatggtact cccatttcat
     2941 ttgctacctc atttttgaat tttgataatc cgttttttgc ttctggaact aaatgttgtg
     3001 acattttgaa ttcctccttt aagtttaata agattttttg aaaggttaag tttttctttt
     3061 cctttcaata ttatgatgtg caaaattgga tttaataatc tataaactta tatccaatat
     3121 aggattaaaa atcacattat ggtataaaat tacaagtgaa attgatttat cttatgaaag
     3181 taaaattaaa catatttttg aaaaaagctc aaacagaatt attatctctt cgagcttttt
     3241 aatttataca ttaaatttta aatctttatg tgataggaat ccatatgaag aatcagcttc
     3301 tttaacagcc tttatgatag ctttttccat aacttgagca gctaaaagac caactacatt
     3361 tatatctgcc ttaactttat ttgaagataa agtaaatatt gtatcaccat caaacatggt
     3421 gtgagcagga cgaatggttc ttccaaaacc attttgagcc atagatgcaa ttttattagc
     3481 ctcagcttta ttaaaattac cattagtcac tatgcaccca atagtagtat ttcctttaaa
     3541 aacattttta ggattagaat aattagctaa taagcttttc tctatgttca taaaggaatt
     3601 cctatcttgg cttaatccac cagctattat tttattgtta tcattagggt caacaatatc
     3661 acctaggcta ttaactccta caatagctcc tacctttaaa tctccgattt gaactgcata
     3721 gcttccaaaa ccgcctttca tagcaagttt ttgatttaag gctttaccta ctgtggcacc
     3781 tgttccacaa cctatattac cgttttttga gtcaaagtag gtttcagaat ttttacaagc
     3841 ttctaagccc attttcttat taggtcttac tttaggatta ccacaggcta aatcaaataa
     3901 aacagcacca catactatag gaactttagt tacccctacg tcaaatccaa catttttatt
     3961 ttctagatat tccatgaccc ctgagcagga atcaagtcca aaggcagatc caccagataa
     4021 tactacagca tgaactttat caaccatttc cataggattt aataaatcag tttctctagt
     4081 tccaggagca ccaccgcgaa catcaactcc tccagaagca ccttcttcac atagtaatac
     4141 tgtacatcct gtagcacctt caaaatcttg agcgtggcct agtttaaaac catctatatc
     4201 tgtaatcttt atttcaaaca ttataatata acctccatta aattgtttta tatgatttag
     4261 atattatatt ttaagaataa tattctattt tacataagag ataatttaca gagtttcaaa
     4321 ttaatgtaaa gtttaaaaat taaatcttta ctaataactg aataaagttt tagcgtataa
     4381 ctttaagtaa gctatacgct aaaatataat ttataatctt aaattatctt aaaatttttc
     4441 taactcctaa tataacagga attgatatta aggctgatat aaatgcctca ggaattccat
     4501 ttgtaactcc tattcctgct aaagttaatt ttgcagcatg ttcagatatt cctagagcag
     4561 aagcatatct ctctaaataa aaaacatatg ttaagccaag tacacctatg gtatttgtta
     4621 aggttccaat tacggaagct gtacctatag ataaggcttc ttctttattt ttatgcttaa
     4681 atttaaacat aaaaatcata cttgctaaaa agaataaaac accgattaca gctgatatag
     4741 ttaaagaatc tgttaaagct tcagaactta caaatagaag aactccaaga ataagagaac
     4801 ttataattga tgtagctttt aaattctttt tagatccttt aatcatagat acatacacgt
     4861 agtagcttat aacacctata agaattctag ggactaaagc tataatagga ttccagaaca
     4921 taaaactagt tggcgttgat gccgtaaaag cttgatacat agaaaataat ccaaaaacta
     4981 atcctattaa tcctcctaca ataggaccct caataatagc tcctattata acaggaatat
     5041 gcataatagt agctttaaca ggtggaatat ttataaatcc taatcctgtc aaccctagaa
     5101 atattgatat accagatagc ataccaataa ctactagttt ttttgttttt tgtctagttt
     5161 gatagactgc attcattgac tctcctccgt tcttattccc agcgggatat aattcgattg
     5221 ttaaaatttt aactgtgaaa aatagttcag cttctaagta tagaataccg acagtgatat
     5281 tttaacatta aatttttaat aaattcaata taaaaaggaa attttgtgca aatttataaa
     5341 ttccataaaa ggtagaatag cttatattaa ggaattagaa gtataaagtg gtaattttct
     5401 gacaattaat atatggataa atttaagtta ttggtaaaaa tgtgattaat taaaatcaaa
     5461 tataattatt aattgactga agttgaatag aaaactaatg gaaattatat atttagaaaa
     5521 ataagtaatt gatttttata atgattactt atataatgtc tattgtaaat taaaattcaa
     5581 tatgttcgga tgaaggtaat gggagagtga tattttaaat gtccaccgaa gagggaaatc
     5641 tttcaggtat taggaccgtt actggacgag cctctggaga gactctttta aaaaagagca
     5701 ccgaaggagc aaggtcaaat ttttgactga aactctcagg taaaaggaca gaggataagg
     5761 ttagttactt aaatttttta agtatcctta ttctcctccg caaatttaaa tttaaggagg
     5821 atttttttat ggaaaactta ttaaatcaaa tcgacaattt agtatgggga gtgccacttc
     5881 ttatgctgct tgtaggaaca ggaatatacc tgactataag actaaaatta ttacaaattt
     5941 taaaattacc attagcctta aagtatgtat ttaaaaaaga tgatgaatct acttgtgagg
     6001 atgcagaagg agatgtttca agctttggag ctttatgtac agccctttca gcaactatag
     6061 gtactggaaa catagttggt gtggccactg ctataaaggc aggaggacca ggagcattat
     6121 tttggatgtg ggtagctgct ttctttggaa tggcaactaa atatgcagaa ggtgtacttg
     6181 ctataaagta taggattgta gatgaaaatg gtcaaatggc tggtggacca atgtactata
     6241 taaaaaatgg acttggttta aactggttag ctaatatttt tgcttttttt gggatttgcg
     6301 tagctttatt aggaatagga acttttggac aagttaaatc cataactgat gcagcaagta
     6361 ttacatttaa tgttcctaca attattacag caggagtagt aactttatta gtggctttag
     6421 taattttagg tggaataaaa agaatatcta gcgtatcaga aatggtagtt cctttaatgg
     6481 caggacttta tatattagga gttttaattg ttatagcttt taatttagat aaggttccac
     6541 atgcagtatc aataattatt gaaagtgcct ttaatcctaa ggctgcttta ggaggagctg
     6601 taggagttag tataataact gtaatgaaaa gtggaatagc tagaggggtt ttctctaatg
     6661 aagctggtct tggaagtgct ccaatagcag cggcggcagc taagactaag tctccagtta
     6721 agcaaggact tatttcaatg acaggtacat tctttgatac aattcttatt tgtacaatga
     6781 caggtatagt aataattctt actggtgctt atagtggaag tttagaagga gcagcactta
     6841 caacacaggc ttttgaaata ggtcttccta taagtaatat aggaacatat atagttaata
     6901 taggacttat gttctttgca tttactacaa tattaggatg gaactattat ggagaaagat
     6961 gtattgagta cttatttgga ataaaagcta taaaaccata tagaatttta tatataattt
     7021 tagttgctat aggatcattc ttaccattaa cattaatatt tataattgca gatattgtta
     7081 atggattaat ggcaattcca aaccttgtag gtattattgg attaagaaaa gtagtaatag
     7141 aagaaacaga ggaattcttt agggaaaaag ctttaagtga agagagtgct gaattagaag
     7201 gaactgcttt aaattaatat aaataaaaaa tccatgagta atttttactc atggattttt
     7261 ttatcttcct ttattaggaa gtttacttct aactatattt tcaacttttt caggaagctc
     7321 tgtaatttct tcctttgtaa gtttatcagt ataaactggt ggatgaatat aaagatttac
     7381 tttaccaggt tttatgaaat aatgatttcc ttccattatt ttatatgttc catcaattgt
     7441 aagtggaact ataggaacct ttggtttagt agctaattta aaactaccag ctttaaattc
     7501 tcccatttta ctacattgac ttcttgttcc ctcaggaaaa acaaccattg aatatccttc
     7561 ttttaattgt ttaattcctt gtataatacc tgccatagct tttttaggat gttccctatc
     7621 aataaatata gcctttgaaa gtattatcca atcttttaat ataggcattt tttcaagttc
     7681 tttttttgct atgaatccct ttggtctatc aatagagtac ataattacag gaatatccat
     7741 attgctttga tggttacaaa caaaaagaac aggtccatca ggaatattct ctaaaccttc
     7801 aacattaact tctattccag catccttaag aaaagtatga gctattcttc tactgcattg
     7861 atatatgtat tcatctcttt cctcaagctt accttcctct tccattttat ttattttttt
     7921 tatatcagat aaggatttta ttaatgtcca ggcatatcct tttccatacc aatatataga
     7981 tctaaacata tatttcgccc ctttaatatt actagtacta agtaatatta taacagtttt
     8041 taaaaaatat caaatatgga atttattttg tggagagcaa taataaaaaa gtgttgatat
     8101 ttactatttc ttttttatag aatataattt atcaaaatag agttatttta tttgagataa
     8161 tttaaataga aaataaatca gtataactta ttttagaata taaattactt tgattggaga
     8221 atacattaat atgaaaaatt tagatttgaa aaatggagta ataatagcag atggagctat
     8281 gggaactaga ataatggaat taggagtaaa tttaaaagaa actccatcag aacttttaaa
     8341 tattaaaaag cctgaattaa tagaaaaaat tcatagagag tatatagaat ctggagctaa
     8401 cttaatactt tcaaacacat ttatgtgtaa tattataaat gcaaaaagga ataattataa
     8461 tttagaggaa gttatagagg ggggaattag tagtgctaaa aaagcatgtg gagatcatgg
     8521 cttagttgct ttagatatag gacctctttc atattatata gaagaaaatg attcttcttt
     8581 taaagaaatt gtttatgaaa atactgaaag aataatcaat gcttcaaaag ataaatttga
     8641 tttagttatt tttgaaacct tagggagttt aaaggaagga gaatttgcag ttaaaaaagc
     8701 aaaaacttta acagataaaa aggttatatg ttcttttact ttagcttata aaaaggatat
     8761 tcctaatttc ataaaaaata tggtttcaac tttagagcct ttaggagtag atgccttagg
     8821 aataaactgt acagggtacg aagaaatact aatggcctta gatatactta aggaaaatac
     8881 aaatctccct ataatgataa aggctaattt aggaatacca aaaaaagtag gggaagagtt
     8941 tatatatgat aagactttag aagaatttaa aaatttatct aaaagagctc ttgaaaaggg
     9001 agttaatata ataggaggat gttgtggaac aactccagaa tatataagaa ctatttgtaa
     9061 tttgaaatag agtataaaag aataatggta taataaggag atataattat gggtgaaaag
     9121 aatagagaaa aagttttttt agaatttatt agtatatgtg tttattcact aataaaaagt
     9181 gatattaaag ttaaataatc tataaaaagt ccactaaaca tataagattg aaaattgaat
     9241 gaatgctttg taaacgtata taatctaggt ataggttaat gaactttaga taaggagagg
     9301 ttaggcatga aaaaagtaca tgtagtttca catagtcact gggatagaga atggtatata
     9361 ccatttcaaa aacatagagt taaattagta gattttatgg attcattaat aaatacctta
     9421 gaaaataatg agcattttaa atattttcac ttagatggac aaactatagc tttagatgat
     9481 tatttagaaa taaagccata tatgtttcat agattaaaga aacttataga agagggaaga
     9541 cttcatgtag gaccatggta tgttcttcaa gatgaatatt taataagtgc agaagcaaat
     9601 gtaagaaata tgcttttagg aatgagattt gcaaaaagat ttggaaatcc agtaaaaata
     9661 ggttatttcc cagattcctt tggtaacata tctcaagcgc ctcaaatact aagaggattt
     9721 ggaatagata atgcagtatt cggtagagga ataactccaa tagcttttga taatcaaaag
     9781 gttgaagaag caggagaaaa tgaatatcac tcagaggtat tatggaaatc accagatgga
     9841 tcagaagttt taggaatact tatggctaac tggtatagta atggtaatga aattccagca
     9901 ggtaaaaaag aagccattga atttttaaat gatagagtta ataaagcaga tatgtatgct
     9961 acaacaaatc agatattatt aatgaatgga tgtgatcatc agccagttca aacaaatgtt
    10021 ggtgacataa tagataatgt aaaagaagat gttgattttg aattagttca tagtaattta
    10081 gttcaatata tagaagatgt taagaaagaa gttaaagact taaaagaagt tacaggagag
    10141 ttaaatggtc aatatacaga tggctggtgg actctatgta acacagcttc atcaagactt
    10201 tacttaaagc aaatgaataa taagtctcaa gttcttttag aaagatatgt tgagccttta
    10261 ggagttatgg cttcattaga aggaaagaaa tacgaaagtg atttcatact tcaagcatgg
    10321 aaatacttaa ttcaaaacca tcctcatgat tcaatctgtg gatgtagtat tgatgaagtt
    10381 catcaagata tggtaacaag atttaaatct agtatgcaaa tatcagagga aatgatagct
    10441 agaagtactg agcatatgtt aaatctagta aatggaacta ctactgatag agaaagcatt
    10501 gttgtattta ataccttagg atgggataga agcgaggtag tagaggctac agttgatgta
    10561 aataaagaat caggtataga aaacttcaag gttgttacag aaaatggaga ggaagttcca
    10621 tcagtggttg aagacttagg agaaacattt aaatatactc ttccaaataa tagatttaga
    10681 caacctgagt atgtaaatag atttaaggtt aaattcttag ctaataatat aaaagcttta
    10741 ggattaaaat catttaaaat agtttcatgt gaaaagagag aagaaaaaga agtttccaaa
    10801 gatttgactt ttgaaaataa attcttctca ttaaagttca atgaaaatgg tacatttgat
    10861 ttaattcata aaggaacaaa taaagagtat aagaatttaa atgcctttga agattgtgga
    10921 gatataggag atgaatacat ctttagagct ccaaagaaag atttagtagt aaatacttta
    10981 ggaaataaag cttctattaa tatattaaaa gataatgaag tagaaaaagt aatagaggtt
    11041 aaaaatactt tagaagtacc agtaaaagct attaagaaag aaagtagaag agtagaagaa
    11101 atgactgaaa ttaagttagt ttctactata atcttaagtg aaatttctaa gagagttgat
    11161 gttaaagttg aaatagaaaa tactgcaaag gatcatagat taagagcctt attcccaact
    11221 ggaatagaag caagcactca ttttacagag ggacattttg atgttataga aagagaaaca
    11281 actccatggg aaggatggca aaatccatct aattgtcaaa aacaaacaaa ctttgttgat
    11341 gtaaatgacg gaaaagttgg acttatgatt gctaacatgg gattacctga atatgaggta
    11401 ttaagagatg gtaaaaatac tatagcttta acaatattaa gatctgttgg agagttagga
    11461 gattggggag tatttaaaac gccagaggct caatgtttag gaaataatgt tgctgaatat
    11521 tcaataatag ctcatgaagg agattatgct ggagaaagag cttacgaaga agcatataac
    11581 ttaaatactc caatgttagc tcttcaagga gattttaaaa aagaggattc tttagtttca
    11641 attaagggag aaaatgtagt tttatcagcc tttaagaaag ctgaagaggg tgattctata
    11701 ataataagaa tatttaacat ttctcataag gaagaaaatt tccaaataaa agttaataga
    11761 gatgttaaag aaataaatga agttaactta aatgaagaac ttatagaaaa actagaagta
    11821 aaagaaaatg ctattaatgg aattatttca aagaaacaaa taaaaacttt tgaaatagta
    11881 atttagtaag taataagagg gtgtatagtt ttatatatcc tctttattaa tttcatatgt
    11941 cttttattaa agaacagttg tattttaaaa tgtggggtga tatactttag ttgtgtataa
    12001 ttaaagatta aaaccttgat tttatcgtat atagaatatt taaaaaatac atctaattat
    12061 atagagtaaa taaaataaaa atattttaat tagtattttt attttagtgt taaagtattt
    12121 ttaaatttaa tttataaatg atgttttttt attgaaaata ttaattatag gtggtatttt
    12181 aataagtagc gaataaaaga gggctattat tttacttgga ttttggatat tttataggtg
    12241 taaagaggtg aatagttcca atggaaggaa atttattagt tatagataaa agagtattac
    12301 cagaagtttt tgaaaaggta ataaacgcta aaagactttt aaaagaagga aaagttaagg
    12361 agataacaga agctacaaag caagctggta ttagtagaag tgtttattat aagtataaag
    12421 actatatatt tgaatttgca gaaacccttc aaggtagaaa agttatattt aatatggttg
    12481 ttactcatga aaagggagtt ttatcatctg ttttaaatat attatcagat gttgggggaa
    12541 atatacttac aatagatcaa gggctaccta tacatggact agctcatgta agcttcacaa
    12601 tagacatatc aactatgaag tgtgatataa aggaaatgtt aaatgaaata gaattagttc
    12661 acggtgttga aaaggtagaa tttgttgcca tggaatagaa aaattcctaa cttagaataa
    12721 tctaagttgg gaattttttt gaaatttatg tatttggtag aaaatgtttt tgataaggat
    12781 aattattatg gtttaataat taatatgata taagaaatgt gtatatataa ttatgtatat
    12841 agaaattata taagtataag gagggtgaag atggaagttt attttgataa tagttctact
    12901 actaagtctt taaaggaagt tagagatgaa gtttattatg ctatggatga attctggggt
    12961 aatccatcat ctttacataa attaggggta aagatgcaaa gaaagattga agagcttcaa
    13021 gagaggattg caaaaaaaat aaatgcttct aaggaagaaa taatttttac ttcaggagga
    13081 agtgaaagca ataatatgat tattaaagga ttagctagag aaaataatca tattataact
    13141 acaacctttg aacattctag tgttttaaac acttataggg aattagaaaa gcaaggtgta
    13201 agtgtaacat atttaaaggt taataataaa ggttttatag atttaaaaga attagaagag
    13261 gcaataaata aaaatacgat tttagtatct ataatgcaga ttaacaatga agtgggaagc
    13321 atacaaaaga ttaaggaaat aggaagatta attaaagaaa aaagtaaaag agcaaaattt
    13381 catgtagatg gagtacaggg ttttggaaaa tttgaaattg atgttaaggc atgtaatata
    13441 gatttttatt ctgtttcagc tcataaattt catggaccaa agggagttgg attcatgtat
    13501 atgagaaagg ggttaaattt aaaatcccta ataactggtg gagaacaaca aagaggatta
    13561 agggcaggaa cggaaaatac tccttcgtat atgggcatgg taaaggctat ggatattgcc
    13621 tatgatgcct tagaagactc ttataatcat gtaaaaaatc ttaaggagta ttttatagaa
    13681 aaactttcta aaatagaaaa tgtagtaata aatagtccta gtagtgaaga atatagtcct
    13741 tacatattaa atgtttcttt tttaggaact agatcagagg ttttacttca catcttagag
    13801 gaggataaca tatttgtttc aacagggtca gcctgttctt caaaagcttc cgtagccaag
    13861 ggaagttacg tattaaatgc tatgggatta gaaccaaagt gcattcaagg ggctataaga
    13921 tttagttttt ctagatataa cactttagaa gaagttgatt acaccatagc ttcactagaa
    13981 aaagctttaa aatttttaag gagaataaaa atatgaataa tttaatttta gtaaaatatg
    14041 cctcagaaat atttttaaag gggcttaata aaaataagtt tgagagaaaa ttaaaagaaa
    14101 atataagaaa aaagttaaaa gatatagatc atgaatttat aacagatcaa aatagatggt
    14161 tcataaaatc agaagactta gatggagtta ttgaaagagt aaaaaaggtt tttggagtta
    14221 aagaactttg tttagttact caggttacag gggactttaa ttcaataaaa gaagagggat
    14281 taaagaaaat taaagaaagc aaagctaaga gtttcaaagt agaaacaaat agagctaata
    14341 aaaaattccc catgaattct atggaggttt caagagctgt tggaggatat atcctttcag
    14401 aacttgggga tgaaatagaa gttgatatac ataatccaga gtgtaagctt tatgtagaaa
    14461 taagaggaaa tgcttatgta tttactgata aagataaaat aaaggctgta ggaggcttac
    14521 catatggaat gaacggaagt actatggtta tgttatcagg aggaattgat tcaccagtag
    14581 cagcttactt aatggctaga agaggagttg aaactcattg tgtatattat cattctcatc
    14641 catacacctc agaaagagcc aaggataagg ttaaggaatt agcaaaaata gtaggaagat
    14701 acacagaaaa aataactctt tatgtggttc cttttacaga aatacaaatg gatataatag
    14761 agaagtgtag agaagatgaa ttaacaataa taatgagaag attcatgatg agagttgctt
    14821 gtgaactttc tgaaagaaag aaaatacagt caataactac tggagaaagt atagggcaag
    14881 tagcatctca gactatggaa ggacttatgg taagtaatga tgtttcagat agacctgtat
    14941 ttagacctct aatagctatg gataaagagg atataatgga tatagcaaga gatatagata
    15001 cttatgacac atcaatactt ccatatgaag attgctgcac aatatttgta ccaaaacatc
    15061 caaagactaa gcctagagtt aaggacatga taatagcaga aagaaagctt gatatagaag
    15121 ctttagtaaa taaggctatt gatgaaatgg aaactttcat atttgaataa ttagtttttt
    15181 ataagcttat ttattgtgtt ttaaagaaat taaatataaa ataaaagggc gtgtttcaaa
    15241 ataagtttta ttactgaatt ttgatacaaa cccttttgtt ttttaggagg agttattaat
    15301 ggagagaaat attgaaaaaa gtataggaaa aatatttaga cattttaaag gtgatctcta
    15361 cttagtagag ggagtagtta ctcattcaga aagtggagag aagatggtat tatatagagc
    15421 tttatatgga gagtgtggtc aatttgtaag accttatgat atgtttttag aagaagttcc
    15481 aaaggaaaaa gaaaatccta caggacagaa atatagattt caagaatttg aagttaaaag
    15541 cttaaataga aaataataat attatttatt aatagaatgt ttaagttcta ttttaaattg
    15601 ataacatttt aaaattaaaa taacgtaata gctcagaaag tgttttaaaa tacttctgag
    15661 ctatcttaat gaataagagg ttacagcaga ttagctataa cgaaaaatga gtttagagaa
    15721 atttaaaacc ctaaaataat aacgcgtaca ttttagaata tggttttaaa gtaactctct
    15781 aaagtagaga aatattatat aggaacagct gtatagatta aataacccta cagttagtat
    15841 tattgtcaaa tatattaaat tatatacaaa tttaaaaatt atttttcatt accaaagatt
    15901 taggtttgat atgataatca ataacggata aaaattgatt ttaataaaag tataataaaa
    15961 taaaag
//
LOCUS       DAEVGI010000046        15668 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEVGI010000046 DAEVGI010000000
VERSION     DAEVGI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 15668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15668
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.046"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..711
                     /locus_tag="K8M77_001254"
     CDS             <1..711
                     /locus_tag="K8M77_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBI7085759.1"
                     /translation="IGSFLNVCIFRIPAGESIAFPPSHCGTCKKQLKPIDLVPVFSYL
                     FLGGKCRHCKSKISIRYPLIELLTGFLYFCVYKYYGISFLTVKYIVLITFLIVISFID
                     YDTQDVYVVTTYPAIILGIIFAIIEKFYFGENLLNYFMGLVISALVIFLISKLTGAMG
                     SGDIEIHAIAGIFLGWKLAIINIFLSFIIGGIIALLAILFKQKKKGDYIAFGPAIGIS
                     TIILIFFGNIIIPIYFGV"
     gene            780..2471
                     /gene="tadA"
                     /locus_tag="K8M77_001255"
     CDS             780..2471
                     /gene="tadA"
                     /locus_tag="K8M77_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBI7085760.1"
                     /translation="MISYQKKRLGDILIESGKLTEDRLKAALLIQKQVGKRLGEILVE
                     QNFVTEEDIIEVLEKQLNIERVNLEIITIDRRAIKMISENVCRKHLIVPYEIDDNTIK
                     VAMADPLDIVAIDDVEISTGLNVRPYIAVKQSIQRTIDIYYSNQKVLLAAEELTKASD
                     EESNELNLDKIEEIDNLDNAPIVKMIEYVIKNAVEERASDIHIETYENILRIRYRIDG
                     KLHTISTLPVDTLAPLVTRVKILANLDISEKRLPQDGRILTKVGDKQIDLRVSILPMI
                     TGEKIVIRIINKENFIISKEGLGLKNFELSLLDKITKHSNGIILVTGPTGSGKTTTLY
                     TILNELNTESKNIITIEDPVEVSIEGINQVNVNTKVGLTFATGLRSILRQDPDTIMVG
                     EIRDTETAQIAIRSAITGHLVLSTLHTSDAPSSVLRLEDMGVQRYLIANAIKGVIAQR
                     LVRKICPNCGQTYEATPYEKEILNIDVNEEVILRRGKGCAYCNNTGYVGRMGIYEIME
                     VTKEHRDAILENKSADILKEISIKHGMRTLGQSCRSLVLEGNTTVEEMLNITYDM"
     gene            2498..3703
                     /locus_tag="K8M77_001256"
     CDS             2498..3703
                     /locus_tag="K8M77_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBI7085761.1"
                     /translation="MANFKYKAINSEGQRIEGSQSADSESQVREMLLSNQYYPLSIEK
                     ENSKNKISFSFNRKVKLKDIAIFCRQFYVMLDSGLSIGKALNILIEQGEKPKVREALI
                     GVNGDLKRGETLANSMRKRKDVFPNLLTSMIDAGERSGNLDIILKRMAEYYEKETKIR
                     GKIKSAMIYPIVLGVVAIIAITFILTFVMPTFVQMFEENNVDLPTSTKMVLGTSKMLG
                     KYGIIIFLILVTAIILLGKYLKSEEGQYKLSIINLKIPVIKKLTQKIIVSRFTRTMGI
                     VSSSGMSLVTSLEIVASVVGNKIAEKELLKVKEKVLKGEGLGDSIMKIKIFPPMLASM
                     VKVGEEAGSLDSILDKTADFYDDELEREIQTATALIEPAMIVVMGIIIGFLLISILTP
                     MFKMYNSIG"
     gene            3775..4320
                     /locus_tag="K8M77_001257"
     CDS             3775..4320
                     /locus_tag="K8M77_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI7085762.1"
                     /translation="MNTKKQNKKKKGFTLIELIIVIAIIAILAAIAIPNFLSIQRKSR
                     VKADIASAKTIYDATSALIAEGKIIPGTQLNTVTEIETNPNAKDDVTDIQGYLQTTPK
                     PKSIDGGVFAVEVSGKEDSPVIKVYIQANGKDYLVYPDGKEPYDLNGDGTGTPKEETK
                     SSNTTPAPSNTSASATNEKNN"
     gene            4439..5593
                     /gene="pilM"
                     /locus_tag="K8M77_001258"
     CDS             4439..5593
                     /gene="pilM"
                     /locus_tag="K8M77_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBI7085763.1"
                     /translation="MAGENKEKKSILKMDVSDIIKAFKKKNSEKKSDKKGSKKKAKSV
                     ICKKAISIDIGSNNTKIIVGRYHKNKVIIDKAFSFKTPKDSIDDGHIKDIDSLARAIT
                     DALDSNNVKNDNVIFTTNSTSVINRTIIIPKVNEDEIESVIKYEVQQYLPINLEDHMI
                     QYNVLEEKVIDGNEKLEVLIVVYPNKMVYSYAELVNKIGGKPYALDLNYNSKRKAYYV
                     MNPEIKESILSIDMGAENIGLTIMKDNELILIKTTKSGGNYLNSKISKVLEISLENAE
                     MQKRENCNLMNREEGPLEHVVREVVDSWFDEASRIIKYYKSKNTQNNIDKIYICGGSS
                     NIKGLERYVSSKLNMKVKIVQGNSNIEFKKGVEEVNISEYINAIGALIRL"
     gene            5608..6174
                     /locus_tag="K8M77_001259"
     CDS             5608..6174
                     /locus_tag="K8M77_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBI7085764.1"
                     /translation="MRDFNFFSHITEERKSDSKRKYIGFGIIGALAFIFVVNFSVNML
                     KEHVLNKNISYYQGEIDNPDMKEKLGVAIKVEKEHDDLEKYYNNVKVATGQVYDNNYV
                     TTQRIKIINSTVPKDLVFTQISIDNKTLTIQALSKTRSAISDLQYNLNNLGFIENTYI
                     SGIGERNAQGDYSFSISCTLKEVGSNEN"
     gene            6164..7381
                     /locus_tag="K8M77_001260"
     CDS             6164..7381
                     /locus_tag="K8M77_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085765.1"
                     /translation="MKINKREKTLLFVLALGVVGFGYYKVVWDYQYNKLKDLKSKELK
                     VKQEYNDDVKMVNSIEPNKEEINIFNSEIQNLTSGFYSNISQPNIILELNNLMNDTNV
                     KGTMSFSEIKTMPVMDKQEGDSSSKSENDENKNQIQGIVNDYNNITDKKKNEDKKNKK
                     QEEIYNLNQMTVSLSVNGTYDNVMKFIKSIEENPKHINILNFNLSAQTDGNVSANMNI
                     QLVAIPKIDASKEEFTTADEKYGKENPFSGASVVGTGTIENELENSKVKNDFLMTVRP
                     INSDFPTIVLGKSGDKDKKTYLNNDENSVSNIEMYISGDNGKYYYKYKVEGKSYPTKF
                     EGNGEEFKPNGNDINFEIFSEKRVNKDDKSGANIKIVNSSDKEVNLIINKDDEKSPRV
                     NVTTEGKVKVIKN"
     gene            7402..8550
                     /locus_tag="K8M77_001261"
     CDS             7402..8550
                     /locus_tag="K8M77_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI7085766.1"
                     /translation="MGIKKRKSKKGFTVIEAIISLVLFTLIMIPLGSFTLTAVKTSAK
                     SATKEQAINAGQGVIEQLKTIKLSQFKKIDSDANYSESNKATLKLGNLNVIKTKDDPT
                     YKIEGEYVDPNNKKKFNIDGDITQGKLEDNGESGKEHTLDKKEPKKNYVVYIGESLIT
                     VYKIVSKENILKKYIDNNTIKIIDDKDNDLEEAKIVNGIFKGSSIKLRRWLLIKNKEL
                     KNSDNEITTLFIDENSNGEINIKAENEDKGEEISTPDSPSEERKKLVKILNEYTDDFE
                     LEDGEKKTDIMVYFYRSSANKKIDLKVSNKCSGSRLNIYTCKNEGSGISYNIYTSTEN
                     SHGNISVFKNYIEGNSEDLRGQLFNINLKIKEKDEILYNLNTTEFIGG"
     gene            8555..10816
                     /locus_tag="K8M77_001262"
     CDS             8555..10816
                     /locus_tag="K8M77_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085767.1"
                     /translation="MFYREKRKGATLAYVIVVLAIIAVIGTAIVSLSLTNYKAMIVHG
                     KKTQNLYMSESGLDKVQKALDILTAKAITYGNDAVAKEDTSLSAEEDLKSGGNKTEDG
                     YKKISSEKEESQNKIFKKAYKEYIIKQLNPNSEVIKNEKVFLLENNKVKFNNNLIKMD
                     KEDKNKEKNEEGNYDITLIDINNKKVSNGSGLNGILNFKTIPDDDKKEEISLRLDSKF
                     NVKELDGDKNYKDVSLTFNIEVPDYNGAYKIEEFPLYNVCSNGLVVGENLNVGTGSTL
                     NVDGGIFVSANVVKEKKFTIGSMVNKKEGDGVVINGGTLSVNNGSLVSKNDIRLGGEG
                     SNLKVGCETENNKSNLNLNDESKKSHSNLNNNLGVYTGNLGINGDKIGFDKDVSKGNI
                     ISNYPVYAMNDLILNGEKSSINLNEGFYGINSNRTEDKLTGKINNSSAIIVNTDDIGS
                     GSSINIGQEAIIMGTAYINTKPEAYQTGESVSVKGNYIAYTVDNGNVEFKYYNPLQLA
                     KLDSVDEKAKYFKDNIKNAKETTHLEGISLPDNTTSIGATISNNEVKLNNKNATLDNI
                     TDISINQVEKYEKAINYIGSLINTKNNDNSEIEFPDKEVRKIVDIKDSDGKLTKAIIY
                     IDSENDKGGNLTIKYGNSTDVTDNEISLGNGIGAGIIVTKKNVILDSKDKSLKFNGTI
                     ITADNLDVKESKKIDLVYDDNLIKKIISQNYDSFKNLFRGQVCNNFITIKSQTNMSDN
                     TLNLVKTSNWEVN"
     gene            10843..11433
                     /locus_tag="K8M77_001263"
     CDS             10843..11433
                     /locus_tag="K8M77_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI7085768.1"
                     /translation="MKNLKFKRRGLSLIELVVALSLIAIILMIVGPFFISNYVTLDKT
                     SNQIDFQREAKAIMNYFTDSAMEASNIESLTNIKNEVLSNNNYTESLKKDSLKSKEGY
                     LKLTFNNSKNKNEHTTFILKDKSLYMKKNDSEEFKIGDLVSSIELTSLTEESTQDKEE
                     INNFKNANAIKIEITFDTKKNTPYKVSNILTFRNKN"
     gene            11455..15567
                     /locus_tag="K8M77_001264"
     CDS             11455..15567
                     /locus_tag="K8M77_001264"
                     /inference="COORDINATES: protein motif:HMM:NF024909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBI7085769.1"
                     /translation="MNLLKTKRRVNFAIISLLLIFSMAGMVSYGIVFNNPKFNIKINK
                     SEDVTISENGTGTVNINDGDSINISYNIEPEKLSIEEKPIDAVVIVDTSKTNCNNITN
                     VKAAVTKFANDFITGSNRRLGIVEYNGSASIVRELNSSKYIDVIEKYKKEDINHLGTN
                     TGDALRLAKAILNKENNNNKKVIILMTNGFANAYTGKFFKSEGDISKKDFWMSNYNNN
                     KDIENYDINKYNIISSFGSSYYHGDIGFNNNGIQYDGVNTYLSEKYFYLGDDEINNRM
                     WSGQREVNENFNESLKEWNNLIGYDDEKNNLEEDIKKANDIIYDINNYIYRTHISWWG
                     YYDIGSKINEVNYNINELNSLLRINYINTLSQINYYIKKEDYNCISNKIDKLETCLNE
                     IYKCTKDSNISIRGNKNLKNYQEFENEKFTETQYFNNILGQIYSCYMAKEVADSGITM
                     YPMFFGDVNEVKNKDLIGLKDGQVKELDLNGYKEKVQKCGDENSDSGILIKSDDEFEV
                     GGNRYKGYSRYGTVYQLAEFGDATKNILVGNNNPKSLENKFNEALNMVKSEVTVKTIL
                     DFDKLPKGVSIDDVKVLGGDKVSIKDIGNGKYQLTITYKKDENNNFNANNFNIEVTYK
                     GHNDTGSILKQDVEAKIINSANNNEIAKFPNLTFNIGQRLATIDIRDSRGKVGSTYNS
                     SKNNINLGKATEATEIFGQGYADIKVKGIGDKKKEYYFKYNFIDEDNNSIKDGTLPLH
                     SDGNIINTSDVDIGKEGNLNVMFYENLIPSSDKTEKTWTDRNKLFQKSKGKSEILPAT
                     ISYYKNSNSIFFDSVNNKGLDFGKYYSAINKYWGYIQPEKSGWYVLGAYADDGVRAWI
                     TTDNKKIDFTNNDWGIDFAGYPGNDQGFDNGIVYLRSNFSPHRPTYYSSFDPIYLEKD
                     KYYPIYVEYYNYEDSGAFELNYNYYGNTEPKYIRNMSNDINNTINYYKKYYTLEDMPE
                     IHDKWLVDFKKNIKFMQQGSGFKFYPSKNTTPGEISSATFSGEHNLMLPFSKKTYKIT
                     YEVIEKSNNNEKSVLKGEYGYFKNNEIFEATISSDENIYPGKEFTLNYNITPKEVPFN
                     EGDKESISVNSINITGKLPDDIKLVTDDVENSEYKIYKNEDSKFTISFKKPMNFKHNG
                     SKYVFDGRDKKISIPIKVKASYNIRPNKEGININPSENILSYKCGDSDELITQYFNGK
                     LDIKNNPSTIESIGLLDYDNKTNKSIINETANNIAGGIPSKVAVEVDAKSPETSVILK
                     IDKGTINHKTLKLYEVDENNNIISTKIDIGENNIKTDDDGKSINIDLNDILNKNNFVK
                     KKYVIVSEIITPKVEAKEDSQSLNVTASIENNPNSTKNKEFISGEMPDVF"
BASE COUNT         6662 a         1437 c         2766 g         4803 t
ORIGIN      
        1 attggaagtt ttttaaatgt gtgtattttt agaattcctg caggggaatc tatagcattt
       61 ccgccttcac attgtggtac atgtaaaaaa caattaaagc ctatagattt agtacctgtt
      121 tttagttatt tgtttttagg tggaaagtgt agacattgta agagtaaaat atctataaga
      181 tatccactaa tagaattgct tacaggtttt ttatattttt gtgtatataa atattatggt
      241 atatcttttt tgacagttaa gtatatagtc ttaattacat ttctcatagt aatttcattt
      301 atagattacg atacccaaga tgtttatgtt gttactactt acccagctat aatacttggg
      361 attatttttg ccataataga aaaattttat tttggagaaa acttattaaa ttactttatg
      421 ggattagtaa tatcagcttt agtaatattt ttaattagta aattaactgg agctatgggg
      481 agtggagata tagagataca tgccatagca ggaatttttt tagggtggaa attagcaata
      541 ataaatatat ttttatcatt tattattggg ggaataatag cactattagc tattttgttt
      601 aaacaaaaga aaaaaggtga ttatatagcc tttggaccag ctataggaat atctactatt
      661 attttaatat tttttggaaa cataataata ccaatatatt ttggtgtgtg aaaaatttaa
      721 tatactagtt tagattaagt tttatagtta aatttttcgt ttgaaaagga ggcatttttt
      781 tgattagtta tcagaaaaag cgtttaggag atatactaat tgaatcggga aagcttacag
      841 aggataggct taaagcagca ttattgattc agaagcaagt tggcaagagg cttggagaga
      901 tcttagttga acaaaacttt gttactgaag aggatataat agaagtttta gaaaaacaat
      961 taaatattga aagagtaaat cttgaaatta taacaataga tagaagagct ataaagatga
     1021 tttcggaaaa tgtttgtaga aagcatttaa tagttccgta tgaaatagat gataatacca
     1081 taaaggttgc aatggcagat cccttagata tagttgcaat agatgacgtt gagatttcta
     1141 caggtttaaa tgtgcgtccg tatatagctg taaaacaatc tattcaaaga actatagata
     1201 tatattattc aaaccaaaag gttttattag cagcggaaga attaacaaaa gcttctgatg
     1261 aagaaagcaa tgaacttaat ttagacaaaa ttgaggaaat agataactta gataatgctc
     1321 ctattgtaaa aatgatagag tatgttatta aaaatgcagt tgaggaaaga gctagtgata
     1381 tacatattga aacttatgaa aatatattaa gaattagata tagaatagat ggtaagttac
     1441 atacaatatc tactttgcca gtagatactc tagctccttt agttactcgt gttaaaatcc
     1501 tagctaactt agatatatca gagaaaagac ttcctcaaga tggaagaatt cttactaagg
     1561 ttggagataa gcaaatagac ttaagagtat ctattcttcc tatgataaca ggagaaaaga
     1621 tagttataag aataataaat aaggaaaatt tcataatttc taaggaagga cttggactga
     1681 aaaattttga gctttctctg ctagacaaaa taacaaagca ttctaatgga ataatccttg
     1741 ttactggtcc tacagggagt ggtaaaacaa caacactcta tactatttta aatgagctaa
     1801 acacagaaag taaaaatatt attacaatag aagatcctgt tgaagtatca attgaaggaa
     1861 ttaaccaagt taatgttaat actaaagttg gcttaacatt tgcaactgga ttaaggtcta
     1921 tattaagaca agaccctgat accataatgg ttggggaaat aagagatact gaaacggctc
     1981 aaatagcaat aaggtcagct attacaggtc acttagtttt atcaacttta catactagtg
     2041 atgctccatc tagtgtttta agattagaag atatgggagt tcaaagatat ttgattgcaa
     2101 atgctattaa gggagttata gctcaaagac ttgttagaaa aatttgtcca aattgtggac
     2161 aaacatatga agctacacca tatgaaaaag aaatattaaa tatagatgta aatgaagaag
     2221 taatacttcg tagaggaaaa gggtgtgctt attgtaataa tactggctat gtaggtagaa
     2281 tgggtattta tgaaattatg gaagtaacta aggaacatag agatgctatt ttagaaaata
     2341 aaagtgcaga tattcttaag gaaatttcaa ttaagcatgg tatgagaact cttggacaat
     2401 catgtagaag tttagtcttg gaggggaata ccacagttga agaaatgtta aatataactt
     2461 atgatatgta atttggtgaa agaaaaggag aggaattatg gcaaatttta aatataaagc
     2521 tattaattca gaagggcaaa gaatagaagg ttctcaaagt gctgattctg aaagccaagt
     2581 tagggaaatg cttttatcaa atcaatatta tcctctaagt atagaaaaag aaaatagtaa
     2641 aaataaaatc agtttttctt ttaatcgtaa ggtaaagctt aaggacatag caatattttg
     2701 tagacagttt tatgttatgt tagattctgg attgtctatt ggtaaggctt taaatatatt
     2761 aatagagcaa ggtgagaagc caaaggttag ggaagcctta attggagtaa atggagattt
     2821 aaaaagaggg gaaactctag caaactctat gagaaaaaga aaggatgttt ttccaaactt
     2881 acttactagc atgattgatg ctggtgagag aagtggtaat ttagatataa tcttaaaaag
     2941 aatggctgag tactatgaaa aagagacaaa gataagagga aaaattaaaa gtgctatgat
     3001 ttatcctata gttcttggag tggtagctat tattgctatt acttttattc ttacctttgt
     3061 aatgccaacc tttgtgcaga tgtttgaaga aaataatgta gaccttccaa catcaactaa
     3121 gatggttctt gggacaagca aaatgttagg taagtatgga attataattt ttttaatttt
     3181 agtaacagca attatattac ttggcaaata tttaaagagt gaagagggtc aatacaagtt
     3241 aagtataata aacttaaaaa ttccagtgat aaagaaatta actcaaaaaa taatagtttc
     3301 tagatttaca agaacaatgg gaatagtatc atcaagtgga atgtctttag taacctctct
     3361 agaaatagtt gcctctgtag ttggaaataa aattgcagag aaggaattac taaaagtaaa
     3421 agaaaaggtc ttaaaagggg aaggattagg agattcaata atgaaaataa aaattttccc
     3481 accaatgctt gcttctatgg ttaaggttgg agaagaagca ggttcactag atagcatatt
     3541 agataaaaca gcagattttt atgatgatga actagaaaga gaaatacaaa cagcaacagc
     3601 cttaatagag ccagcaatga tagttgttat gggaataatt ataggatttc tactaatttc
     3661 aatattaaca cctatgttta aaatgtataa cagtataggt taagaggata attaacaaga
     3721 attaaaagtt tagcttttaa tataaattac atattatttt aaggaggaaa accaatgaat
     3781 acaaaaaaac aaaacaaaaa gaagaaagga tttacgctaa ttgaacttat tatagtaata
     3841 gcaataatag caatattagc agcaatagca ataccaaact tcttgtcaat tcaaagaaaa
     3901 tcaagagtta aggcagatat agcaagtgct aaaacaatat atgatgcaac atcagctttg
     3961 attgctgaag gtaagataat accaggaaca caattaaata ctgtaactga aattgaaact
     4021 aatccaaatg ccaaagacga cgttacagat atacaagggt atcttcaaac tacacctaaa
     4081 ccaaaatcaa ttgatggtgg tgtatttgca gtagaagttt caggcaaaga agattcacca
     4141 gttattaagg tttacattca agcaaatggt aaagattatt tagtttatcc agatggaaaa
     4201 gaaccatatg atttaaatgg tgatggaact ggtacaccaa aagaagaaac taaatcttca
     4261 aatacaacac cagcaccttc aaatacatca gcatcagcaa ctaatgaaaa aaataattaa
     4321 tagtattaga agttttaata ttagtgaaat aataaggtga ttttttaatt ataatgaaaa
     4381 aagtgtaagg aattttttcc ttgcactttt caactataaa ggcaaaggag ggaaagtttt
     4441 ggcaggtgaa aataaggaga aaaagagcat attgaagatg gatgtttcgg atataatcaa
     4501 agcttttaag aagaaaaatt ctgagaaaaa gagtgataaa aaagggagta agaaaaaagc
     4561 taagagtgtt atatgtaaaa aagctatttc tattgatatt ggaagtaata atacaaagat
     4621 tattgttggg agatatcata aaaataaggt tattatagac aaggcttttt cttttaagac
     4681 tccaaaagat agtatagatg atggtcatat taaggatata gatagtttgg ctagagctat
     4741 tactgatgct ttggattcta ataatgttaa gaatgataat gtaattttta ctacaaacag
     4801 cacttctgtt ataaatagaa caattattat tccaaaggtt aatgaggatg aaatagaatc
     4861 agttattaag tatgaagttc agcaatatct tcctataaac ttagaggatc atatgattca
     4921 atataatgtt ttagaggaaa aggttattga tgggaatgaa aagctagagg ttttaattgt
     4981 ggtttatcct aataagatgg tttattccta tgcagagctt gttaataaaa ttggtggaaa
     5041 accatatgct ttagatttaa attataattc taaaagaaaa gcttattacg taatgaatcc
     5101 tgaaataaag gagtctattt taagtattga tatgggagct gagaatatag gtttaacaat
     5161 aatgaaagac aatgagttaa tacttataaa aactacaaaa tctggtggta attatttaaa
     5221 tagcaaaata tcaaaggtct tagaaatttc attagaaaat gcagaaatgc aaaagagaga
     5281 aaattgtaat ttgatgaata gggaagaagg acctttagag catgttgtta gagaggttgt
     5341 agattcttgg tttgatgagg ctagtagaat aattaaatat tataaaagta aaaatactca
     5401 aaataatata gacaagatat atatttgtgg aggaagttca aacataaagg gactagaaag
     5461 atatgtatca tctaaactaa atatgaaagt aaagatagtt caaggaaata gcaatataga
     5521 atttaaaaaa ggtgtagaag aagtgaatat ttcagaatac ataaacgcta ttggagcctt
     5581 aataagactt taggttggtg ataaataatg agagatttta actttttctc ccatataact
     5641 gaggaaagaa aaagtgattc aaaaagaaaa tacataggat ttggaataat tggagcctta
     5701 gcctttatat ttgtagttaa tttttcagtg aacatgttaa aagagcatgt tttaaataaa
     5761 aacataagtt attatcaagg agaaatagat aacccagata tgaaagaaaa gttaggtgta
     5821 gcaattaaag ttgaaaagga gcatgatgac ttagaaaagt attataataa tgtgaaagtt
     5881 gctacaggac aagtttatga taataattat gtaactactc aaagaattaa aataataaat
     5941 agtactgttc ctaaggattt agtatttact caaataagta tagataataa aactttaact
     6001 atacaagcat tatcaaaaac aagaagtgct ataagtgatc ttcaatataa cttaaataat
     6061 ttaggtttta tagaaaatac atatataagt ggaattggag aaagaaatgc acagggagat
     6121 tattcattct ccataagctg tactttaaag gaggtaggaa gtaatgaaaa ttaataaaag
     6181 agaaaaaact ttattatttg tcctagctct aggggttgtt ggttttggat actataaagt
     6241 ggtttgggat tatcaatata acaaattgaa agatttaaaa tcaaaagaac taaaagtaaa
     6301 gcaagaatat aatgatgatg tgaaaatggt taattcaata gaacctaata aggaagaaat
     6361 aaatattttt aattcagaaa ttcaaaactt aacatcaggc ttttattcaa acatatcaca
     6421 gccaaatata atattagaat taaataatct tatgaatgat acaaatgtta aaggaactat
     6481 gagtttttct gaaataaaaa ctatgccagt tatggataag caagagggag attcttctag
     6541 taaaagtgag aatgatgaaa ataaaaacca aatacaggga atagttaatg attataataa
     6601 tataactgat aagaagaaaa atgaagataa aaaaaataaa aaacaagagg aaatatataa
     6661 cttaaaccaa atgacagttt cattaagtgt aaatggtact tatgataatg ttatgaagtt
     6721 tattaagtct attgaagaaa atccaaagca cataaatata cttaatttta atttaagtgc
     6781 acaaactgat ggaaatgtat cagcaaatat gaatatacaa cttgttgcaa ttccaaagat
     6841 agatgcttca aaagaagagt ttacaacagc tgatgagaag tacggtaagg aaaatccatt
     6901 ttcaggagca agtgtagttg gaacaggaac aatagaaaat gaattagaaa attcaaaggt
     6961 taagaatgat ttcttaatga ctgttagacc tataaattca gatttcccaa caatagtttt
     7021 aggtaaatca ggtgataaag ataaaaaaac atatttaaat aatgatgaaa attcagtaag
     7081 taatatagag atgtacattt caggtgataa tggaaagtac tattataaat acaaagttga
     7141 agggaaaagt tatccaacaa aatttgaagg taatggagaa gaatttaaac caaatggaaa
     7201 tgatataaac tttgaaatat tctcagagaa gagagtaaat aaagatgata aatctggagc
     7261 taacataaaa attgtgaata gttcagataa agaagttaat ttaataataa ataaggatga
     7321 cgaaaaaagt ccaagagtta atgtaaccac agagggcaaa gttaaagtga ttaaaaatta
     7381 atagaaaagg tgtgtgaagc tatgggtatc aaaaaaagaa aaagtaaaaa agggtttaca
     7441 gtaatagaag ctataattag tttagttttg tttactttaa taatgatacc cctaggtagc
     7501 tttactttaa cagctgttaa aacatcagca aaaagtgcca caaaagagca ggctataaat
     7561 gctggacaag gtgtcataga acaattaaaa acaattaaat taagccagtt taagaaaata
     7621 gactcagatg caaattatag tgagagtaat aaagctacat taaagcttgg aaacttaaat
     7681 gttataaaga caaaagatga tccaacttat aaaattgaag gtgaatatgt agaccctaat
     7741 aataagaaga aatttaatat agatggagat ataacacagg ggaagcttga ggataatgga
     7801 gaaagtggta aggaacatac tttggataaa aaagagccga aaaaaaatta tgtggtatat
     7861 ataggagaaa gccttataac agtttataaa attgtttcaa aagaaaatat tttaaaaaaa
     7921 tacatagata acaatacaat taaaataatt gatgataaag ataatgactt ggaagaagct
     7981 aaaatagtaa atggaatttt taaaggaagt agtataaagc ttagacgctg gttacttata
     8041 aagaataaag aactaaaaaa tagtgataat gaaattacaa ctctttttat agatgagaat
     8101 agtaatggtg agataaatat aaaagcagaa aatgaagata agggagaaga gatatcaacc
     8161 cctgatagtc caagtgaaga aagaaaaaag cttgttaaaa tattaaatga atatactgat
     8221 gattttgaat tagaggatgg agagaaaaaa actgatataa tggtgtattt ttatagaagt
     8281 tctgcaaata aaaaaataga tttaaaagtg tcaaataagt gctcaggaag tcgattgaac
     8341 atttatacat gtaagaatga aggaagtgga ataagttata atatttatac ttcaactgaa
     8401 aattctcatg gcaatataag tgtatttaaa aattatatag agggaaatag cgaagattta
     8461 agagggcaat tatttaatat taatttaaag attaaggaaa aagatgagat tctttataac
     8521 cttaatacaa ctgagtttat aggagggtga taacatgttt tatagggaaa aaagaaaagg
     8581 ggcaactctt gcttatgtaa tagtagtatt agctataatt gctgtaatag gtactgctat
     8641 agtttcactt tctttaacaa actataaggc aatgattgtc catggtaaga aaactcaaaa
     8701 tttatatatg tcagaatcag gacttgataa agttcaaaaa gctttagata ttcttactgc
     8761 taaggctata acttatggaa atgatgctgt tgcaaaagaa gatacatcat tatcagcaga
     8821 agaagattta aaatcaggtg gtaataaaac agaggatggt tataaaaaaa tatcatctga
     8881 aaaggaagaa agtcagaata aaatattcaa gaaagcttat aaggaatata ttataaagca
     8941 attaaatcct aatagtgaag ttattaaaaa tgaaaaagta tttttattag aaaataataa
     9001 agttaagttt aataacaatt taattaaaat ggataaagaa gataaaaata aagaaaaaaa
     9061 tgaagagggt aattatgata ttacacttat tgatataaat aataaaaaag tatctaatgg
     9121 aagtggttta aatggaattt taaattttaa gactatacct gatgatgata aaaaggaaga
     9181 aataagttta aggttagatt ctaagtttaa tgttaaagaa ttagatggag ataagaacta
     9241 taaagatgta tctttaactt ttaatattga agttccagat tataatggag cttataaaat
     9301 tgaagagttt cctttatata atgtatgttc aaatggttta gttgttggag aaaacttaaa
     9361 tgttggtaca gggtctacat taaatgttga tggggggatt tttgtatcag caaatgttgt
     9421 caaagagaaa aaatttacaa taggaagtat ggttaataaa aaagagggag atggggttgt
     9481 tattaatgga ggaacactta gtgttaataa tggctctttg gtaagtaaga atgatataag
     9541 acttggtgga gaaggaagca atttaaaagt tggttgtgaa acagaaaata ataagagtaa
     9601 tttgaattta aatgatgaat caaaaaaatc tcatagtaat ttaaataaca atttaggtgt
     9661 atacacagga aatttaggga taaatggaga taagattggt tttgataaag atgttagtaa
     9721 aggaaatatt atttctaatt atcctgttta tgcaatgaat gatttaatat taaatggaga
     9781 gaaatcatca ataaatttaa atgagggttt ttatgggata aatagtaata gaactgaaga
     9841 taaattaaca ggtaaaataa ataattctag tgcaattata gtaaatacag atgatatagg
     9901 aagtggatca agtataaata taggacaaga ggccattata atgggaacag cttacataaa
     9961 tacaaagcct gaagcctatc aaactgggga atctgtttct gttaagggaa attatattgc
    10021 ctatacagtt gataatggaa atgtggaatt taaatattat aatccactac aacttgcaaa
    10081 gttagattct gttgatgaaa aagcaaaata ttttaaggat aatattaaaa atgcaaagga
    10141 aactacacat ttagagggaa taagtttacc tgataataca actagtatag gagctaccat
    10201 aagcaataat gaagtaaagc taaacaataa aaatgctacc ttagacaata taactgatat
    10261 tagcataaat caagttgaga agtatgaaaa ggcaattaat tatataggaa gcttaattaa
    10321 tacaaaaaat aatgataatt cagaaattga atttcctgat aaggaagtaa gaaaaattgt
    10381 ggatattaaa gatagtgatg gaaagttaac taaggcgata atctatatag attctgaaaa
    10441 tgataagggt ggaaatctta caattaaata tgggaattca acagatgtaa ctgataatga
    10501 aatatctcta ggaaatggaa ttggtgcagg aataatagtt acaaagaaaa atgtaatatt
    10561 agattctaaa gataaaagtt taaagtttaa tggaactatt ataacggctg ataatctaga
    10621 tgttaaagaa agtaaaaaaa ttgacttagt atatgatgac aatcttataa aaaaaataat
    10681 atctcaaaat tacgattctt ttaaaaattt atttagagga caggtttgta ataatttcat
    10741 tacaataaaa tcacaaacta acatgagtga taatacttta aatttagtta aaacaagcaa
    10801 ttgggaagta aactaagcta ttaagagaag gttggtgatt aaatgaaaaa tttaaaattt
    10861 aaaagaagag ggctttctct tatagaatta gttgtggcac tatccttaat agcaataata
    10921 ttaatgatag ttggtccatt tttcatttca aattatgtaa ctttagataa gacaagcaat
    10981 caaatagatt ttcaaaggga agcaaaggcc ataatgaatt attttactga ctcagctatg
    11041 gaagcatcta atatagaaag cttaactaat attaaaaatg aggttctttc aaataataat
    11101 tatactgaga gtctaaaaaa ggattctttg aaatcaaaag agggctatct taaactaact
    11161 tttaataata gtaaaaataa aaatgaacat acaacattta tattaaaaga taaatcatta
    11221 tatatgaaga agaatgattc ggaagaattt aaaataggag atttagttag tagcattgaa
    11281 ttaacttctt taacagaaga atcaacacaa gataaagaag aaatcaataa ttttaaaaat
    11341 gctaatgcta ttaaaattga aattacattt gatactaaaa agaatacacc atataaagta
    11401 agcaatatat taacctttag aaataaaaat taagcttgga tggaggtgat taaaatgaat
    11461 ttattaaaaa ctaaaagaag agtaaatttt gctattataa gtttattatt aatttttagt
    11521 atggctggaa tggtttcata tggaattgta tttaataatc ctaaatttaa tattaagata
    11581 aataaatctg aggatgttac tatttctgaa aatggtacag gaacagttaa tataaatgat
    11641 ggagatagta ttaatattag ttataatata gagccagaaa aattatctat tgaagaaaag
    11701 ccaattgatg ctgttgttat agttgataca tctaaaacta actgtaataa cataactaat
    11761 gtaaaagctg ctgtaacaaa atttgcaaat gattttatta ctggatctaa tagaagattg
    11821 ggtatagtag aatataatgg ttctgcaagt attgttagag aattaaatag tagcaagtac
    11881 attgatgtta ttgaaaagta taaaaaagaa gatataaatc acctaggaac aaatactggt
    11941 gatgctttaa gattagctaa ggcaatacta aataaagaga ataataataa caaaaaagtt
    12001 attatactta tgacaaatgg atttgccaat gcatatacag gaaaattttt caaaagtgaa
    12061 ggagatataa gtaaaaaaga tttttggatg agtaactata ataataataa agatatagaa
    12121 aattatgata ttaataaata taatattatt agtagttttg gaagtagtta ttaccatggt
    12181 gatataggtt ttaataataa tggaatacaa tatgatggtg ttaatactta tttatctgaa
    12241 aaatattttt atttaggtga tgatgaaata aataatagaa tgtggagtgg gcaaagagaa
    12301 gtaaatgaaa attttaatga gagtctaaaa gagtggaata atttgattgg atatgatgat
    12361 gaaaaaaata atttagaaga agatattaaa aaagcaaatg atatcatata tgatattaat
    12421 aattacatat ataggacaca catttcttgg tggggatatt atgatattgg gagtaaaata
    12481 aatgaagtta attataatat aaatgaatta aatagtttat taagaattaa ttacataaat
    12541 actttaagtc aaataaatta ttatataaaa aaagaagact ataattgtat aagtaataaa
    12601 attgataagt tagagacatg tcttaatgaa atatacaaat gtacaaaaga tagtaatata
    12661 tctattaggg gtaataaaaa cttaaaaaat tatcaagagt ttgagaatga aaaatttaca
    12721 gaaactcaat actttaataa tattttagga caaatatatt catgttatat ggcaaaggaa
    12781 gttgctgatt ctggaataac aatgtatcct atgttttttg gtgatgttaa tgaagtaaaa
    12841 aataaggatt taataggatt aaaagatgga caggtaaaag aattagattt aaatggatat
    12901 aaagaaaagg tgcaaaagtg tggagatgaa aattcagatt caggaatttt aataaaatct
    12961 gatgatgaat ttgaagttgg tggaaatagg tataaaggat attctaggta tggtactgta
    13021 tatcaattag ctgaatttgg tgatgcgact aaaaacatat tagtcggaaa caataatcca
    13081 aaatctcttg agaataagtt taatgaagcc ttaaatatgg taaaatcaga agttacagta
    13141 aaaacaatac tagattttga taagctacca aaaggagtaa gcattgatga tgtaaaggta
    13201 cttggaggag ataaagttag cattaaggat ataggaaatg gaaagtatca attaacaata
    13261 acctataaaa aagatgaaaa taataatttt aatgctaata actttaatat tgaagttaca
    13321 tataaagggc ataatgatac aggaagtatt ctaaaacaag atgtagaggc aaagattata
    13381 aatagtgcaa ataataatga aatagctaaa tttccaaatt taacttttaa tataggacaa
    13441 agactagcaa ctattgatat aagagattct agaggaaaag tgggaagcac atataactct
    13501 tctaagaata atattaatct tggaaaggct acagaagcaa cagagatatt cggacaaggt
    13561 tatgctgata taaaagttaa gggaataggc gataagaaaa aagaatatta ttttaaatat
    13621 aattttattg atgaagataa taattcaatt aaagatggaa cactgccttt acattcagac
    13681 ggtaacataa taaatactag tgatgtagat ataggcaaag aaggaaatct aaatgttatg
    13741 ttttatgaga acctaattcc aagtagtgat aagacagaaa agacatggac tgatagaaat
    13801 aaattgttcc aaaagtcaaa aggaaaatct gaaattttac cagctaccat ttcatattat
    13861 aaaaattcta atagtatatt ttttgattca gttaataata aaggacttga ttttggaaaa
    13921 tactattcag cgataaataa gtactggggc tatatacagc cggaaaagag tgggtggtat
    13981 gtattagggg cttacgctga tgatggtgta agagcatgga taacaactga taataaaaaa
    14041 atagacttta ctaataatga ttggggaata gattttgctg gatatccagg taatgatcaa
    14101 ggatttgata atggaattgt atatttaaga tctaatttct ctccacatag accaacttat
    14161 tatagtagtt ttgatcctat ttatttagag aaagataagt attatcctat atatgtagag
    14221 tattataact atgaagatag tggagccttt gagttaaatt ataattatta tggtaatact
    14281 gaacctaaat atataagaaa tatgagcaat gatattaata atactattaa ctattataag
    14341 aagtactata cattggaaga tatgcctgaa attcatgata agtggttagt tgattttaaa
    14401 aaaaatataa aatttatgca acagggaagt ggatttaaat tttatccttc aaaaaatact
    14461 actcctggag aaatttcaag tgcaacattt tctggagagc ataacttaat gctaccattt
    14521 agtaaaaaaa cttataaaat cacttatgaa gttatagaaa aatcaaataa taatgaaaaa
    14581 agtgttttaa aaggagagta tggttatttt aaaaacaatg aaatatttga agctactata
    14641 agtagtgatg aaaatattta tccaggaaag gaatttacat tgaattacaa tataactcca
    14701 aaagaagtac cttttaatga gggagataag gaaagtatat ctgttaattc tataaatata
    14761 acaggaaaac taccagatga tattaaatta gtaactgatg atgtagaaaa tagtgaatat
    14821 aaaatatata agaatgaaga ttctaagttt acaatatcat ttaaaaaacc tatgaatttt
    14881 aaacataatg gttcaaaata tgtatttgat ggaagagata aaaaaatatc aatacctatt
    14941 aaggttaagg caagttataa tataagaccg aataaagagg ggattaatat aaatccatct
    15001 gaaaatatat taagttataa atgtggagat agtgatgagt taattactca atattttaat
    15061 ggtaaattag atattaaaaa taatccttca acaatagaaa gtataggact attagattat
    15121 gacaataaaa caaataaatc aattataaat gaaactgcta ataatattgc tgggggaata
    15181 cctagtaaag tagcagtaga agttgatgca aaatctccag aaacttcagt aatattaaag
    15241 attgataagg gaacaataaa ccataaaaca ttaaaattat atgaggtaga tgaaaataat
    15301 aatattatta gtacaaaaat tgatatagga gaaaataata ttaaaacaga tgatgatgga
    15361 aagtcaatta atattgattt aaatgatatt cttaataaaa ataattttgt taagaaaaag
    15421 tatgttattg tttctgaaat aattactcca aaagtagaag ctaaagagga ttcacagagt
    15481 ttaaatgtaa ctgcgagcat tgaaaataat ccaaatagca ctaagaataa agaatttatt
    15541 tctggagaaa tgccagatgt attttaaact aaaagaaaaa taagatcaca taatttaatt
    15601 tgtggtctta tttttgtatt tattaataaa ttttagagca taatatattt aattaagatt
    15661 aaaataaa
//
LOCUS       DAEVGI010000047        15481 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEVGI010000047 DAEVGI010000000
VERSION     DAEVGI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 15481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15481
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.047"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(25..2220)
                     /locus_tag="K8M77_001265"
     CDS             complement(25..2220)
                     /locus_tag="K8M77_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI7085770.1"
                     /translation="MILLKKLVLAEKPSVGREIAKVLKCNNNKGSYIEGKDYVITWAL
                     GHLVELQSPEDYDNKLKTWSMETLPMLPKHMKLKVIKKATKQFYEVKKQMERKDIDEI
                     IIATDAGREGELVARWIIEKAHVKKPIKRLWISSQTDKAILDGFKNLKPGENYNNLYK
                     AAQCRAEADWLVGLNVTRALTCKYNAQLSAGRVQSPTLAMIVQREEDIKNFKPKEYST
                     ILIETDKCNFTWINKDNNSRIFKNDFKEKVVSNLNEKKSGKIININESNKKKFSPQLY
                     DLTELQRDCNKIFGYSAKQTLNIMQRLYENHKLLTYPRTDSRYISKDIVSTIPDRLKA
                     VATGEFRTIANDLLKNPIKANKSFVDDNKVSDHHAIIPTEEKGNLANLSSDERRVYEL
                     VVKRFLSVLMPPFEYIQTTLTGEVNGEKLIAKGKVVKAKGWKKLYDREVDDEGEEDIK
                     EQELPKLKEGDEFKVLKVNVKKGETKPPARFNEGTLLSAMENPQKFISVDKSSAKTLG
                     ETGGLGTVATRADIIEKLFNSFVIEKRGKEIIPTSKGKQLIDLVPKDLKSPLLTANWE
                     EMLERISKGKGDSKKFIKDIRNYTVALVEDVKLGESKFHHDNLTGKKCPQCGKYMLEV
                     KGKNGTMNVCQDRECGYRENISRITNARCPECKKKLELRGHGEGKIYVCPGANCNFRE
                     KESQFKKRFEKNNKTNKREVNKIMQKMKKEANEDLNNPFADLLSGLKFD"
     gene            complement(2308..3726)
                     /locus_tag="K8M77_001266"
     CDS             complement(2308..3726)
                     /locus_tag="K8M77_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBI7085771.1"
                     /translation="MKFYKKNHWCKIFITFFKLMKNTWPVIIFLAFKFNDYIVEITSI
                     SIVLILIFSVVKWFNTSILIEENFLIYREGALLKEEMVIPLRSISLIELERNIIYRIF
                     KLRKIKIDSVYPSSKKNAEIIMVLKKRDLESLYGNLSYRMRNLIHDEREINQTLVYNI
                     SAVHLILLSMLRNNILLGIGVLYSSIHFMSKIYKGLNQELIDFFRNVIEENVISRNTI
                     LAIFLSALFLFSILLLIVLIFSVLAILSKYYKFTIYRNNNYLKVEYGLITRRSYSIPM
                     ESIHAVKIEQNIINQIFKFYTIKCCVVGYGNSIKEDELIFPLCNEKGYRNILKNLIPE
                     FIFEGEVYRPEKKDLRRFFTIPIQVSLYSSIGFFLLTNEIWPLLISVPVVIVERTLLY
                     KNTKIGFGKDLYYVSYKSLNRRQIFIKRSSMEEIRVTSNPLQIRKKLGNFKLRFYSQK
                     KLEWIKLKNLKLEIYDELKKWA"
     gene            complement(3736..4203)
                     /locus_tag="K8M77_001267"
     CDS             complement(3736..4203)
                     /locus_tag="K8M77_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBI7085772.1"
                     /translation="MKYKLSKLFIVKSIILRFLIFSLLYLGLSLGFYRLKSFSSFNQL
                     NGIMDNTLWVLQLLITVIFIISVLICPIIKYFTWSYFIFSDFIEIRYGVLFKKYICIK
                     RNNIKYINVCENPIDMILRIKSINIYTAGGKVSIPAINKEQVEFFNYIVQERV"
     gene            complement(4620..5018)
                     /locus_tag="K8M77_001268"
     CDS             complement(4620..5018)
                     /locus_tag="K8M77_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085773.1"
                     /translation="MLDLKRCAIKMASNVKVVDSKRGKVTLKNDLILRGLRNNCEYEE
                     IIKEAIMTMKEINKVEFFYEKLNLVISYDCTVTSEKKVVIWIKSIIEIILDNYEELKS
                     IVEKNNKEELINFILPELKKKINLNKYKVK"
     gene            complement(5214..8474)
                     /locus_tag="K8M77_001269"
     CDS             complement(5214..8474)
                     /locus_tag="K8M77_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBI7085774.1"
                     /translation="MNIKKLVIRYAFLSLILILIQGKNVLALGESEVRFSNLTLKDGL
                     SQSTVNKIIQDNKGYVWFGTSDGLNKYNGHDFVIFRELVNDKNSINSNFISALIKYDN
                     DNILVGTSKGVSKINVITNEVSRMFEDEDGKSKLSNSNIWDMLKHSDGTIWIATRKGL
                     NLYDPKTGEVYEIFKDTEPKGLKSNVVTSLCEDKYGNVWVGTSEGLSVINTKTKGIQS
                     INDMLDKDNSSISNLYRDKDDNIWVVTDNHIMSVSSNYKDITYFDKELESFKISPDSV
                     NAIYEDSYNNLWISTQNGLLEYNKQNKKMHFFESKYYDDTSLVNDNVLTSYEDRSGLL
                     WFGTYNGISLLNPNQPFKHYRREPDKENTISDNSMSGIYKDSDGMLWLGTNSAGLNKL
                     NTKTGEVVNFYSDNSDENTLSSNVVWQICEADKDNLWVATQGGVNKINKKSGNVERLK
                     YPENMRERNKMDARNVFVDKDGLLWIGARGGLQSYDDKTRTFTDYANVFKSVGIVENA
                     VTSISQDEEGFLWITIGIDGGVVKFDKSKGIVKSYLNNSFDSNSLINNGVRCLEKDSK
                     GNIWIGTEAGLSKLDPKTDKFTNYTVVNGLANNFIYGILIDEEDNLWISTNDGLSRFD
                     QKANKFINYTAMDGLQSNEFNGRSYFKGKDGEMFFGGINGVTAFYPKDVLKLSNSRNK
                     SKVVIDKIKVNNKTYPYYGGTLNLKYNENNISIDFFLPDYLNTTKTTYEYKLEGVDEE
                     WTFANKRNNATYATLDSGTYKVLIRARDNHGDLTPVTALDLKIDPPPWRTPTAYLIYF
                     SSAVIVIFLMWSYVTALEKTVKKRAEQLKKEAKEKEKLYKEAAELKDEMIKRQKFKND
                     YFVNLSHELRTPLNVILATVQLIKLKNKKECLGKYQVEDYTNTLRNNADHLLKIINDI
                     IDSSKIESGAYKLNIEKHDIVSLVEDTALSLKAYIEENNLDIIIDPEIEELDVECDKN
                     EIERCVINLLSNAIKYTHGHGTIEVFIRECENETVEIAIKDTGVGISKEQQKIIFDRF
                     VQADSIAKKQYRSSGIGLTLVKSIIELHHGKIKVESELNKGSTFIITLPIRQEKSK"
     gene            complement(8556..9656)
                     /locus_tag="K8M77_001270"
     CDS             complement(8556..9656)
                     /locus_tag="K8M77_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085775.1"
                     /translation="MKKHPIIYMFFSFIMILTFFDIVKKDNSFSELENRNLARKPIFS
                     ISSFLDGKYRDKYEEYINDNFIFRNKWINLKSISEYTLGKLENNNIIYGKENYMFEKN
                     ISYDKERFKNNIDSINKFIDIYEGKVTTMLVPNSYTIYEEYIPWGIKLLNKENLINEV
                     YKANDKDRNIDLVSLFKKNKEEYIYYKTDHHWTSYGAYLAYKAYIESLGEKPIDLNSL
                     KKNEATGFYGTYFSKAKLFSAESDILTYYDIDNITMNIQGKIFESLYDYDKLKTRDKY
                     SVFIRGNNGFSIIENKNIKLGKKLLIFKDSFANSMIPFLSQNFQEIHVVDLRSFSWKV
                     SEYMKNTNFNEILILYNVENFLRDINISKIKY"
     gene            complement(9671..11038)
                     /locus_tag="K8M77_001271"
     CDS             complement(9671..11038)
                     /locus_tag="K8M77_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBI7085776.1"
                     /translation="MVFSSIIFIFRFFPIMFIIYYLTPNKFKNLSLLILSLIFYSWGE
                     ARYFFIILLSIGVDYSISIFIEKYREREIISKVLLFCSIMFNLGMLILFKYLNFFIEN
                     INLIFGNSLSLIYLTLPLGISFYTFQTMSYTIDVYRGKIRAERNIINFGAYVCLFPQL
                     IAGPIVKYSDIKNELKNRKIKVNQVEEGITEFIIGMGKKVLIANNIGILWNEIQELGI
                     NYVSTPLLWLGVIAFSFQIYFDFSGYSSMAIGLGKMLGFDFPENFNFPYISRSITELW
                     RRWHITLGSWFKEYVYVPMGGNRLGKNRTILNLFIVWFLTGFWHGAKYNFIIWGLYFF
                     FLIVIEKVFLMNYLNKYKIFSHIYAIVFIVFGWALFSFEDLLSIKYLFRMKFSLDFIY
                     YLRNYFLVLIIAILGSTKLINNIYERIIKKNKSLECLVLMMVFILSVAYLVDSTYNPF
                     LYFRF"
     gene            complement(11265..11762)
                     /locus_tag="K8M77_001272"
     CDS             complement(11265..11762)
                     /locus_tag="K8M77_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBI7085777.1"
                     /translation="MKKFFISVLASFMIGTTLTSCGAKDNNGTKVEQSDIKMSMSASD
                     MTNKLVDAGMVTMPVPLDDEIAKEIYHLNLSDIEEYGISETGISPGNSLIIIAKAKEG
                     KVESVKASLDKVLEDKIGKAFYPEEKESAESSEVKVKGNYVSLFILPEDQLEEANKIY
                     EGEFK"
     gene            complement(12014..13231)
                     /locus_tag="K8M77_001273"
     CDS             complement(12014..13231)
                     /locus_tag="K8M77_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7085778.1"
                     /translation="MDKFLKLGLSEEVLKSLVGLGIEEPTDIQEKAIPEILNGKNVIG
                     KAETGTGKTLAYLLPIIEKIDDSKNEMQSIILSPTHELGVQINNVLNDLKRGLGKKIT
                     STTLVGSGNIKRQMEKLKNKPHILVGTTGRILELINKKKITTNTIKTIVIDEGDKLLD
                     FINIKDVKSVVKSCPRDTQKLIFSATMNEKALENADELIGTSELIQAKAANKVNENIE
                     HGYFQVELRDKIDFLRKLIHAIGDEKKIIVFINNSYNVHNVIQKLKYNKIEAVSLHGS
                     DNKMERKKALQDFRSGKAKVLITSDVSARGLDIKGATHIVNLDIPMNSQNYLHRVGRV
                     GRAGEKGFAYSLADYKEEKIIVKCERQLKIKIPRVYLYEGKIHETEVKKVSSNKNNSK
                     KKSNLPKKVYKKR"
     gene            complement(13255..13566)
                     /locus_tag="K8M77_001274"
     CDS             complement(13255..13566)
                     /locus_tag="K8M77_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085779.1"
                     /translation="MAKITSRNNDFLKMHRNSTSPKVYSLLIELINEDREDLANEVIK
                     IDYLVDYFNTCIKKRDKREGKETLERINLRLSKLKNEGVDTSHFETLCENILKNNKIK
                     L"
     gene            complement(13876..15222)
                     /gene="gdhA"
                     /locus_tag="K8M77_001275"
     CDS             complement(13876..15222)
                     /gene="gdhA"
                     /locus_tag="K8M77_001275"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBI7085780.1"
                     /translation="MEVKKYVDNLMEDLKKNNPGESEFLAAAEEVLYSLVPVLEKNPK
                     YIEEGILERIVEPERVIMFRVPWVDDAGNVRVNRGYRVQFNSAIGPYKGGLRFHPSVN
                     LSIIKFLGFEQIFKNSLTTLPIGGGKGGSNFDPKGKSDREIMRFCQSFMSELYRHIGP
                     NTDVPAGDIGVGGREIGYMFGQYKKLKNSVDAGVLTGKGLTYGGSLARKEATGYGLVY
                     FVDEMLRDNGQTIEGKTVVISGSGNVAIYATEKVQELGGKVVALSDSLGYVYDENGID
                     LEIVKEIKEVKRGRISEYVNYVKTAKFTEGFSGIWNVKCDIALPCATQNEIDKSSAKT
                     LIDNGVIAVGEGANMPSTLEAQKLFVDNKILFAPAKAANAGGVATSALEMSQNSLRMS
                     WTFEEVDAKLKDIMKNIYYNSKNAASEYGHNGNLIVGANIAGFKKVADAMLDHGII"
BASE COUNT         5105 a         2726 c         1369 g         6281 t
ORIGIN      
        1 atttgatata atttttaaat ttaattagtc aaatttaaga ccacttaaaa gatcagcaaa
       61 aggattattt aaatcttcat ttgcttcttt tttcatcttt tgcattattt tatttacttc
      121 tcttttattt gttttgttat ttttctcaaa tctcttctta aattgagatt ctttttctct
      181 aaaattacaa tttgctcccg ggcaaacata tattttacct tctccatgtc ccctaagctc
      241 tagttttttc ttacactcag ggcatctagc attagttatt ctagatatat tttctctata
      301 tccacactct ctatcttggc aaacattcat tgttccattt ttacctttta cctcaagcat
      361 atacttacca cactgagggc attttttccc tgttaagtta tcatgatgga atttgctttc
      421 tccaagctta acatcctcaa ctaaagcaac ggtatagttt cttatatctt ttataaattt
      481 cttagaatcg ccttttcctt tacttattct ctctaacatc tcttcccaat tagctgttaa
      541 aagtggtgac tttaaatcct ttggtactaa atctataagt tgttttccct tagatgttgg
      601 aattatttcc tttcctctct tttctattac aaaggaatta aataattttt ctataatatc
      661 cgctctagtt gcaacagtac caagtcctcc agtttctcct aaagtctttg ctgaagattt
      721 atctactgat ataaattttt gaggattttc catagcagat aataaagttc cctcattaaa
      781 tcttgctggt ggctttgttt ctcctttttt aacattaacc ttaagaactt taaattcatc
      841 gccctctttt aatttaggaa gttcttgttc ttttatatcc tcttctccct catcatcaac
      901 ttctctatca taaagcttct tccatccttt agctttaact acttttccct ttgcaattaa
      961 tttttctcca ttaacttcac cagttaaagt tgtttgaata tattcaaatg gaggcattaa
     1021 tacacttaaa aatctcttaa ctacaagctc ataaactctt ctctcatcag aacttaaatt
     1081 agctaggttt cctttttcct ccgttggaat tatagcatgg tgatcagaaa ccttattatc
     1141 atctacaaaa cttttgtttg cttttatagg attttttaat aaatcatttg ctatagttct
     1201 aaattctccc gtagctacag cttttaatct atctggaata gtagaaacta tgtcctttga
     1261 aatatatctt gaatcagttc ttggatatgt aagaagtttg tggttttcat ataacctttg
     1321 cattatatta agggtctgtt ttgctgaata tccaaaaatt ttattacaat ctctttgaag
     1381 ctcagttaaa tcatataatt gaggagaaaa tttcttttta ttactttcat ttatattgat
     1441 tatttttcca cttttcttct catttaaatt agaaacaact ttttctttaa aatcattttt
     1501 aaatattctt gaattattat ccttatttat ccatgtaaaa ttacatttat ctgtttctat
     1561 taaaatagta ctatactctt ttggtttaaa gtttttaata tcctcttctc tttgaactat
     1621 cattgctaag gtaggtgatt gaacccttcc tgctgaaagc tgagcattat atttacaagt
     1681 taaggcccta gtaacattta aaccaactaa ccagtcagcc tcagctctac actgagcagc
     1741 cttatataaa ttattgtaat tttctcctgg tttaagattt ttaaatccat ctaagatagc
     1801 tttatctgtt tgagaggata tccaaagtct ctttataggc tttttaacat gggctttttc
     1861 tattatccaa cgtgctacaa gttctccttc tcttccagca tctgtggcta ttattatttc
     1921 atctatatct tttctttcca tttgcttttt aacttcataa aattgcttag tggctttttt
     1981 aataacctta agtttcatat gcttaggaag cattggaaga gtttccatag accatgtttt
     2041 aagcttgtta tcataatctt ctggagattg taattctact aaatggccta aagcccaagt
     2101 tattacataa tcctttcctt caatataact tcctttatta ttattgcatt ttaaaacctt
     2161 ggcaatttct ctacctacac taggtttttc tgctagtact aatttcttca ataaaatcat
     2221 cctttcatga tactacatat atgtaatatc tctaataata tactacctta aatatattta
     2281 cttaacaaat aaaatcctat atttttatta ggcccatttc tttagttcat cataaatctc
     2341 taattttaag ttttttaatt taatccactc aagtttcttt tgggaataaa atctcagctt
     2401 aaaattacca agcttttttc ttatttgaag tggatttgat gttactctta tttcttccat
     2461 actagatctt tttataaaaa tttgtcttct attaagagat ttataactaa cataatataa
     2521 gtcctttcca aaacctattt tagtattttt atatagcaaa gttctctcaa caataactac
     2581 aggcacactt attaaaagag gccatatctc attagtcaat aaaaaaaatc ctatgctaga
     2641 atatagtgaa acttgtattg gaattgtaaa aaatcttctt aaatctttct tttctggcct
     2701 gtacacttcc ccttcaaata taaactcagg aataagattt tttaatatat ttctatatcc
     2761 cttttcatta cataaaggaa atataagttc atcttcctta atactatttc catatccaac
     2821 tacacagcat tttatagtat aaaacttaaa tatttgattt ataatatttt gctctatttt
     2881 tactgcatgt atgctttcca ttggaattga atagcttctt cttgttatta atccatactc
     2941 aacttttaaa taattattat tcctatatat tgtaaactta taatacttag ataatattgc
     3001 taagacagaa aatatcaata ctattaataa taaaatacta aataaaaaaa gtgctgataa
     3061 aaatattgct aatattgtat ttctagatat tacattttcc tcaataacat tcctgaaaaa
     3121 atctattaac tcttgattca atcccttgta tatcttactc ataaaatgta tgcttgaata
     3181 taaaacccct attcctaata atatgttgtt tctaagcatt gaaagtaaaa tcaaatgaac
     3241 agctgatatg ttgtaaacta aggtttgatt tatttctctt tcatcatgta ttaaattcct
     3301 catcctataa cttaagtttc cataaaggga ttctaaatct ctctttttta ataccattat
     3361 tatttctgca tttttctttg aagaaggata tacactatca atctttatct ttctaagctt
     3421 aaaaattcta tagattatat ttctttcaag ctctattaag gatatgctcc ttaagggaat
     3481 caccatttct tccttaagta gagccccttc tctgtatatt aaaaaatttt cttcaattaa
     3541 aatacttgta ttaaaccatt tcaccacaga aaaaattaaa attaacacta tagaaataga
     3601 ggttatttcc acaatataat cattgaactt aaaagctaaa aatattatta ctggccaagt
     3661 atttttcata agcttaaaaa aggttataaa aattttacac caatgatttt ttttataaaa
     3721 tttcattttt cacccctaaa ctctctcctg aactatataa ttgaaaaatt ctacctgctc
     3781 cttattaatt gctggaatac taactttccc tccggcagta taaatattta tgctctttat
     3841 tcttaaaatc atatctattg gattttcgca tacatttatg tattttatat tattcctttt
     3901 tatacatata tattttttaa ataacactcc atatctaatc tcaataaaat cactaaatat
     3961 aaaataagac cacgtaaaat acttaattat tggacatatt aatactgata taataaatat
     4021 aactgtaatt aataattgta aaacccataa agtattatcc ataattccat ttaattggtt
     4081 aaagctacta aaacttttta acctataaaa tcctaagctt aagcctaaat ataagagtga
     4141 aaaaattaaa aatctaagaa taatagattt aacaataaat aatttactta atttatattt
     4201 cattaactcc tcctttaata aaaaaaacct ataagaaaac cattcttata ggctacccgt
     4261 tgccaaaaat ctataaatat ctataaatct tgtttaattc cctgttctac gagtcagatt
     4321 ttgaaaacta tacagagtgt aatctacttt catttcttat aactctcaga gagcgtaaat
     4381 tcggatacaa cgcatcctac atataaaagt tatcttgttt ttgatatttt ttgtattact
     4441 atatttgctt ggttttcgct tctaagtact cacttagatt ttctttaaac actaaaaccc
     4501 tcaaggtttt accctatgac tatatcactt tcgcaatata tgggattcac aatagctaat
     4561 aatattttac cacattataa tacttattta aatcattata tattcccata ttttcaaagt
     4621 tactttacct tatatttatt taaattaatt ttctttttaa gttcaggtaa aataaaattt
     4681 atcaattctt ctttattatt cttttccaca atacttttta attcctcata attatcaaga
     4741 attatttcta ttattgactt tatccaaatt accactttct tttcagaggt aactgtgcaa
     4801 tcataagaaa ttactaaatt taatttttca taaaagaatt caaccttatt tatttccttc
     4861 attgtcatta ttgcttcttt aataatttct tcatattcac aattatttct taatcctctt
     4921 aatattaaat cattctttaa tgtaactttt ccccttttac tatcaactac ctttacattt
     4981 gaagccattt ttattgcaca cctttttaaa tcaagcatat ttttctccct aaataatatt
     5041 ctttatctaa agcttatgat taattccact ttaaaattcc ataaagataa tataaaattt
     5101 aaaagagagc ttatcaaaat agctttaaat gaaacttatt agaaagatta aatctttact
     5161 tgtttataag ttttattaaa gttctcttaa tatactctct tttttaaaat tagttattta
     5221 cttttctctt gtcttatagg taatgtaatt ataaaagtac ttcctttatt aagttcactt
     5281 tctactttta ttttaccatg gtgaagttct attatacttt ttaccaatgt taagcctata
     5341 ccactgcttc tatattgttt tttagctatg ctatcagctt gtacaaatct atcaaatata
     5401 attttttgtt gctccttgga tattccaact ccagtatcct ttatagcaat ttctacagtt
     5461 tcattttcac actctcttat gaaaacttca attgtaccat gtccatgagt atattttata
     5521 gcattagata aaagattaat aacgcatctt tcaatctcat ttttatcaca ttctacatct
     5581 aattcttcta tttcaggatc tattataata tctaaattgt tttcttcaat ataagccttt
     5641 aagcttaaag ctgtatcctc aactaaagaa actatatcgt gcttttctat atttaattta
     5701 tatgcacctg actctatttt agatgaatct attatatcat ttattatctt aagtaaatga
     5761 tctgcattat ttctaagagt attagtataa tcttccactt gatattttcc tagacattct
     5821 ttcttattct ttaactttat taactgcact gtagctaata ttacatttaa tggagttcta
     5881 agttcatgtg aaagatttac aaaataatca tttttaaact tttgtctttt tatcatctca
     5941 tctttaagct ctgccgcttc tttataaagc ttttcttttt ctttagcctc ttttttaagt
     6001 tgctcagctc tttttttaac agttttttcc aaagcagtta cataactcca cattaagaaa
     6061 ataactatta ctgcacttga aaaatatatt aaataagccg ttggtgtacg ccatggtggt
     6121 ggatcaatct ttaagtctaa agcagttact ggtgttaaat ctccatgatt atccctagct
     6181 ctaattaaaa ctttatatgt tccagaatct aaggtagcat aagttgcatt atttctttta
     6241 ttagcaaaag tccattcctc atcaacacct tctaacttat actcataagt agtttttgtt
     6301 gtgtttaaat aatctggcaa aaagaaatca atagatatgt tattttcatt atactttaaa
     6361 ttaagagttc ctccataata tggataagtt ttattattaa ccttaatttt atctattact
     6421 actttactct tatttctact atttgataat tttaaaacat ctttcggata aaatgcagtt
     6481 actccattta ttcctccaaa aaacatttct ccatctttgc ccttaaaata agatctgcca
     6541 ttaaattcat tactttgcaa accatccata gctgtataat ttataaattt atttgctttt
     6601 tgatcaaatc tagataaccc atcatttgta cttatccaaa gattatcctc ttcatctata
     6661 agtattccat atataaaatt attagctaat ccatttacaa cagtataatt agtaaattta
     6721 tcagttttag gatctaattt acttaatcca gcttcagttc caatccaaat gttcccttta
     6781 gaatcttttt ctaaacaccg tactccatta tttataagac tattgctatc aaaactatta
     6841 tttaaatagc tttttactat tcccttgctc ttgtcaaatt ttactacacc accatcgatc
     6901 cctatagtaa tccataaaaa tccttcttca tcttgagaaa tgcttgttac agcattctca
     6961 actataccta cagacttaaa aacattagca taatcagtga aagttcttgt tttatcatca
     7021 taagattgta acccacctct tgctcctatc cacaataatc catctttgtc cacaaaaaca
     7081 tttctagcat ccattttatt tctttctctc atattttctg gatactttaa tctttcaaca
     7141 tttccacttt ttttatttat cttattaact ccgccttgag ttgcaaccca caaattatct
     7201 ttatcagctt cacatatttg ccaaactaca ttgctactta aggtattttc atcactatta
     7261 tcactataga aatttacaac ttctccagtc ttagtattta acttattcaa tcctgctgag
     7321 ttagttccaa gccataacat cccatcagaa tctttataaa tcccactcat actattatca
     7381 ctaatagtat tttccttatc tggttctctt ctataatgct taaatggctg attaggattt
     7441 aataaactta ttccattata cgtaccaaac caaagcaacc cacttctatc ttcatatgaa
     7501 gttaatacat tatcattaac taaactagta tcatcataat acttactttc aaaaaaatgc
     7561 atttttttat tttgtttatt atattctaac aatccatttt gagttgatat ccataaatta
     7621 ttatatgaat cttcataaat tgcatttaca ctatctggag atattttaaa agactctaat
     7681 tctttatcaa aataagtaat atctttataa ttacttgata ctgacatgat gtgattatca
     7741 gttactaccc atatattatc atccttatct ctataaagat tagaaattga tgagttatct
     7801 ttatctaaca tatcatttat agattgaata ccctttgttt ttgtattaat tacacttaac
     7861 ccctcacttg ttccaaccca tacatttccg tatttatcct cacataaaga agtaactaca
     7921 ttacttttta atcctttagg ctctgtatct ttaaaaattt catatacctc tcctgtttta
     7981 ggatcataaa gattcaaccc ttttctagta gctatccaaa tggttccatc actatgcttt
     8041 aacatatccc atatattact atttgatagc ttgctttttc catcttcatc ctcaaacatt
     8101 ctacttactt catttgttat tacatttatc ttacttacac ctttactagt accaacaaga
     8161 atattatcat tgtcatattt tattaaagct gatataaaat ttgaattaat actattttta
     8221 tcatttacta attctctaaa aataacaaaa tcatgtccat tatatttatt taacccatct
     8281 gatgtcccaa accatacata tcctttatta tcttgaatta ttttatttac tgtagactgg
     8341 gataatccat cttttaatgt taaattgctg aaccttactt ctgattctcc taaagccaaa
     8401 acatttttgc cttgaattaa tattagtatt aaggagagaa aagcatatct tataactaat
     8461 ttttttatat tcaattttat tacctctcta aaactaaaat aacaatttaa ttttctcaaa
     8521 aaacaagaat attgtcaaac aatgtcacat taaatttaat attttatctt agaaatattt
     8581 atatctctca gaaaattttc tacattatat aatattaata tttcattaaa attcgtattt
     8641 ttcatatatt cacttacttt ccatgaaaaa ctcctaagat caaccacatg aatttcctga
     8701 aagttttgac ttaaaaatgg aatcatagaa ttagcaaagg aatctttaaa tataagtaat
     8761 ttttttccta actttatatt tttattttct attatagaaa atccattatt tcctctaata
     8821 aatactgagt acttatccct agtctttaac ttatcataat catataagct ttcaaaaatt
     8881 ttcccctgta tattcatagt aatattatct atatcataat aagttaaaat atcactttct
     8941 gcactaaaaa gcttagcctt tgaaaaatat gtcccataaa atcctgtagc ttcattcttt
     9001 tttaaactat ttaaatcaat aggtttttct cctaagcttt caatataagc tttatatgct
     9061 aaatatgccc cataacttgt ccagtggtga tcagttttat aatatatata ttcctcttta
     9121 tttttcttaa ataaactcac taaatctata tttctatctt tatcattcgc cttataaact
     9181 tcatttataa gattctcttt atttaaaagc tttattcccc atggaatata ttcttcataa
     9241 atggtatatg aatttggaac tagcatagta gtaaccttgc cctcatatat atcaataaat
     9301 ttatttatgg aatctatgtt atttttaaac ctctccttat cataggaaat attcttttca
     9361 aacatataat tttcttttcc ataaattata ttgttatttt ctaactttcc taaggtgtac
     9421 tcacttatag attttaagtt tatccactta tttctaaaaa taaaattatc atttatatac
     9481 tcttcatatt tatctctgta ttttccatct aaaaaagaac ttattgaaaa tataggtttc
     9541 cttgcaagat tcctattttc tagttctgaa aaactattat ctttctttac aatatcgaaa
     9601 aaggttaaaa tcattataaa agaaaaaaac atataaataa ttggatgctt tttcataaaa
     9661 tatttccttt ctaaaatcta aaataaagaa atggattata agtagaatcc actaaatatg
     9721 ccactgataa tataaacacc atcattaata ctaagcattc taaagattta tttttcttta
     9781 tgattctctc ataaatatta tttattaact ttgtagatcc taaaatagct attataagaa
     9841 ctaaaaaata gtttcttaaa taataaataa aatctaaaga aaatttcatt ctaaataaat
     9901 actttatact aagtaaatcc tcaaaggaaa aaagtgccca cccaaaaact ataaaaacta
     9961 ttgcatatat atgagaaaaa atcttatact tatttaaata attcattaga aaaacttttt
    10021 ctataacaat aagaaagaaa aaatataatc cccatattat aaaattatat tttgctccgt
    10081 gccaaaaacc agttaaaaac catacaataa aaagatttaa tattgtccta tttttcccta
    10141 atctattccc tcccatagga acataaacat attccttaaa ccaagagcca agggtaatat
    10201 gccaccttct ccaaagctca gtaatacttc ttgatatata tgggaaatta aaattttctg
    10261 ggaaatcaaa tccaagcatc tttccaagtc ctatggccat agaggaatat ccactaaaat
    10321 caaaataaat ctgaaaagaa aatgctatta caccaagcca taataaagga gttgacacgt
    10381 aatttattcc aagttcttga atctcattcc atagaatacc tatattatta gctattaaaa
    10441 ccttcttgcc cattcctatt ataaattcag taatcccctc ttcaacttga ttaactttaa
    10501 ttttcctatt tttaagttca ttttttatgt ctgaatattt tactataggt cctgctataa
    10561 gctgtggaaa aagacataca taagctccaa agtttattat atttctttca gcccttatct
    10621 ttcctctata tacatcaata gtatatgaca tagtctgaaa ggtataaaaa cttattccta
    10681 gaggtaaggt taaatatatt aatgataaag aattcccaaa aattaagttt atattttcaa
    10741 taaagaaatt taaatattta aataatataa gcatacctaa attaaacata atggagcaaa
    10801 ataaaagcac cttacttatt atttccctct ccctatattt ttcaataaaa atacttattg
    10861 aataatctac tcctatggat aaaagtatta taaaaaaata ccttgcttct ccccatgagt
    10921 aaaatattaa acttaatatt aagagagata aatttttaaa tttatttgga gttaggtagt
    10981 atattataaa cattattgga aaaaatctaa atataaatat aatactgcta aataccattt
    11041 taaatatcct caaaattaaa ttttatattt ctaagagact ttgatagtct cttttttatt
    11101 atatttctct ctaaatacct tctaatctaa aatatttatt ttaaaataat aaataaatta
    11161 gttaaacttg ctaatactta tagaaaaata taaaatcccc ccatttccta agcatagcaa
    11221 gtttaaaagt caaactatta atttatttaa gtaggaatta acctttattt aaattcccct
    11281 tcgtatatct tatttgcttc ttctaattga tcctctggta atataaataa tgaaacataa
    11341 tttcctttaa ccttaacttc tgaagactct gcactctctt tttcctctgg ataaaaagct
    11401 tttccaatct tatcttctag aactttatct aaagatgctt ttactgactc tacttttcct
    11461 tccttagctt ttgcaataat aattaatgaa tttcccggtg atataccagt ctcagaaatc
    11521 ccatattcct ctatatcact taaatttaaa tgataaattt ccttagctat ttcatcatct
    11581 aaaggcacag gcattgtaac catacctgca tctacaagtt tatttgtcat atctgaagca
    11641 ctcatagaca tttttatatc actttgttca acctttgttc cattattatc cttagctcca
    11701 caagaggtta atgtagtccc tatcataaac gaggctaaga cacttataaa aaactttttc
    11761 atccctatat tcactcccta aaatacataa tttattatgt taacgttatt atatcctata
    11821 acctaatccc atccaataat tttagaatat tgttaatcct ttagtaactt ttaaagctta
    11881 tttttatcat tccatgcctt tcttctaaag gtatattaat taaattcaaa ttaacaatag
    11941 ataaaaatac tatttaaata aaaaaaagca ccaaaagaaa tggcttcttt ggtgcattat
    12001 aatatatatt taattatctt tttttatata cctttttagg taaattactt ttctttttac
    12061 tattattttt atttgaagaa acctttttta cctctgtttc atgtatcttt ccttcatata
    12121 aataaactct aggaattttt atttttaatt gtctttcaca ttttactatt attttttctt
    12181 ctttataatc tgctaaagag tatgcaaagc ctttttctcc agctcttcct acacgtccta
    12241 ccctatgtaa gtaattttga gaattcatag gaatatctaa gttaactata tgtgtagctc
    12301 ccttaatatc tagccctcta gctgatacat ctgaagttat taaaaccttg gcttttccac
    12361 ttctaaaatc ttgaagtgcc ttctttctct ccattttatt atcacttcca tgaagagaaa
    12421 ctgcctctat tttattatac tttaactttt gaattacatt atgtacatta tagctattat
    12481 ttataaaaac tattattttt ttctcatcac ctatagcatg tataagcttt cttaaaaagt
    12541 ctattttatc tcttaattct acttgaaaat atccatgttc tatattttca ttaaccttgt
    12601 ttgcagcttt tgcttgaata agctcactag ttcctattaa ttcatctgca ttttctaagg
    12661 ctttttcatt cattgtagct gagaatataa gcttttgagt atcccttgga caagatttaa
    12721 caacactttt tacatctttt atgtttataa aatctaatag tttatcacct tcgtcaataa
    12781 ctattgtttt tatggtatta gttgttattt ttttcttatt tataagctct aaaattcttc
    12841 ctgtagttcc aacaagtata tgaggcttat ttttaagctt ctccatttgt ctctttatat
    12901 ttccgcttcc aactaaagtt gttgaagtta tcttctttcc aagccctctt ttaagatcat
    12961 ttaaaacatt gtttatctga actcctaatt catgagttgg tgaaagaata atagattgca
    13021 tctcattttt tgaatcatca atcttttcta ttataggaag taaatatgct aaagtctttc
    13081 ctgttcctgt ttcagctttt cctattacat ttttaccatt taaaatttca ggtatagctt
    13141 tttcctgaat atctgttggc tcctctattc ctaatcctac taaagatttt aaaacctctt
    13201 cacttaatcc taactttaaa aatttatcca ttagcatccc atccttcttt aactctaaag
    13261 tttaatctta ttgtttttta atatattctc acaaagagtt tcaaaatgag aagtatctac
    13321 gccttcattc tttaacttag aaagtcttaa atttattctt tctaaagttt ccttaccttc
    13381 tcttttatct ctttttttga tacatgtatt aaaataatca actaaataat ctatttttat
    13441 aacttcatta gctaagtctt ctctatcttc atttataagt tctatcaata aagaatatac
    13501 cttaggagaa gttgaatttc tatgcatttt taaaaaatca ttatttctac tagttatctt
    13561 tgccatactt attttccttt ctaaataaat tatgatttac cataaattat tgacataaca
    13621 tcctttaata tatcataagt aaaaagaaaa aaacagaaga aactatatga aaataaactt
    13681 caaataaaac ttattaatat attttactaa ttactagata aatctataag ctttattttt
    13741 aaagattcaa tatagtttct tcttaaaaca ataatattct ctattttatt attctataaa
    13801 gtttagcttt aatttaaata tcaaacttta taaaatatat aatacttaaa ataacaacta
    13861 ttttaaaagt attttttata taataccatg atctaacata gcatctgcaa cctttttaaa
    13921 tcctgcaata ttagctccaa ctattaaatt tccattatga ccatattcag aagctgcatt
    13981 tttagaatta tagtatatat ttttcattat gtctttaagt tttgcatcaa cttcttcaaa
    14041 agtccaagac attcttaaac tattttgact catttctaaa gctgatgttg ctactccacc
    14101 tgcattggca gcttttgctg gggcaaataa aattttatta tcaacaaaaa gtttttgagc
    14161 ttctaaagtt gaaggcatat ttgccccttc tcctacagca attactccat tatcaattaa
    14221 agtttttgca ctgcttttat ctatttcatt ttgagtagcg caaggaagtg ctatatcaca
    14281 tttaacattc caaattccgc taaatccctc agtaaactta gcagttttaa cataatttac
    14341 atattcagaa attcttcctc tttttacttc tttgatttct tttacaatct ctaaatctat
    14401 tccattttca tcatatacat accctaatga atctgaaagt gcaacaacct ttcctccaag
    14461 ctcttgaact ttttcagtgg catatattgc aacatttcct gaacctgaga ttacaacagt
    14521 tttcccttct attgtttgac cgttatctct taacatttca tctacaaaat atactaaacc
    14581 atatccagtt gcttcttttc tagctaagct accaccataa gttaagccct tgccagttaa
    14641 aactccggca tctactgaat ttttaagttt tttatattga ccaaacatat agcctatttc
    14701 tcttcctcca accccaatat ctcctgcagg aacatctgta ttaggtccta tatgtctata
    14761 aagttcactc ataaaacttt ggcaaaatct cattatctct ctatctgatt ttcccttagg
    14821 atcaaagtta gatccgcctt ttcctcctcc aataggaaga gtagttaaag aattcttaaa
    14881 tatttgctca aatcctaaaa attttattat gcttaaatta actgaagggt ggaatcttaa
    14941 tcctccctta tatggtccta tagcactatt aaattgtact ctatatcctc tgttaactct
    15001 tacatttcca gcatcgtcaa cccaagggac tctaaacatt ataactctct ctggttcaac
    15061 aattctttct aatattccct cttctatata cttaggattt ttttctaaga caggtactaa
    15121 agagtataat acttcttctg ctgcagctaa gaactcactt tctccaggat tatttttctt
    15181 taaatcttcc attaaattat ctacatattt ttttacttcc actttaatgc ccccttttta
    15241 tattcactat aacttttaca tcaactaaat tatttaaaag ttatagcttt aagctaatat
    15301 tctaggaaaa tattttctta attaaatctt attttatatt atatcataaa gtaccattat
    15361 tattaacttt taatacatta tactaaattt tagttattta aattctaata tagtattgtt
    15421 atatatatat tattacttaa ttaaataaag ccatttatac ttttaattta atcttaaaaa
    15481 t
//
LOCUS       DAEVGI010000048        15479 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEVGI010000048 DAEVGI010000000
VERSION     DAEVGI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 15479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15479
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.048"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(116..1639)
                     /locus_tag="K8M77_001276"
     CDS             complement(116..1639)
                     /locus_tag="K8M77_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7085781.1"
                     /translation="MKKSISKRLFIITFGIIIFLTLFTMIFQITFFQEFYYKRKSNDL
                     SNAVLKFRALYSYDIKNTDSLYRALSLFELENNAKIGIYSIDSTPKYVPDPANKNSEA
                     VDLLNDIFNELYSDQDFAKTLLNSNSGVTTEFKSRKYSTKYIVYMVPFSLNSKNDSII
                     IAITSFQAIEEASALIKDFYKYIILIVIVIGLILSYVFSNLISKPLVKLNKSAKKMSA
                     MDFSEKCDIEREDEIGNLAKTLNFLSKNLSNALDDLKIKNKKLEEDIEKERELEKLRK
                     DFIAGASHELKTPIGIISGYAEGIKDGIVDHKDQGVYLDIIIDEAEKMNKLVMDMLEL
                     SKLEAGKIDLHIIDFSLTELTEEVLVKNSVEINKNNLTVIKNYSPTNDLYVQGDDFKI
                     EQVLTNFVTNAIKYSEPNNQIIIDIKSVEEDKIYFSIENTGAHIPDSDINKIWTQFYR
                     GDTSRNRGSRSTGLGLSIVKNLLQAHESEFGVMNTENGVKFYFTLKKSKEIDEVEEI"
     gene            complement(1639..2310)
                     /locus_tag="K8M77_001277"
     CDS             complement(1639..2310)
                     /locus_tag="K8M77_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085782.1"
                     /translation="MNKTVLIVEDEIRIRFLVRDYFKKEGFNVLEASDGEEALRIFSE
                     NIVDLAILDIMMPKLDGLAVCRNIREVSNTPIILLTAKSQEEDKLLGYELGADDYMTK
                     PFSPKVLLAKSKALLRRTGTLNDKNILDFNGLTINKLSHEVKLNGEELLLSPKEYDLL
                     IYLSSNEGIALSRDRILDNVWGYDYFGDIRTVDTNIKRLREKLLDKANYIATVRGSGY
                     KFEVK"
     gene            2405..3649
                     /locus_tag="K8M77_001278"
     CDS             2405..3649
                     /locus_tag="K8M77_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI7085783.1"
                     /translation="MKRWTFDENTIILPNGLKVITIKKNTRLASINIGVNIGSLYEDE
                     KELGMSHFVEHMLFKGTKNRSNEQLNRELEFLGGDYNAYTDYISTVYSITCLDEEFEK
                     GIELLSDMILNSSFDEKEMKKEKGVVLSEIKSDKDDIEDLSISRTHEYAFDKSALRNS
                     IAGTEEHVKGFKRKQVYDFYKKYYTPDNCVIVTVSAFSHEQMQKIITDLFGKWEGKSH
                     KKAKIIKEENKNIVKTTYKSQIEQGTVTYLYTFKEVCEKDKLPLKILSYKLAESSNSI
                     LFRELREERGLAYDVYSQMDLDENVNTMNIFTSVREESIDEVIEVIDKAILDIKNRDI
                     NFDEDMLCMMKKTHKTGVVSTLEDCSSLCSYVLVQSLAGKDITEFINSMEELETLTGE
                     DIYRVCNKYLNKPTIHILKPME"
     gene            3655..4293
                     /gene="recX"
                     /locus_tag="K8M77_001279"
     CDS             3655..4293
                     /gene="recX"
                     /locus_tag="K8M77_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBI7085784.1"
                     /translation="MGKITSIEVQKRNVNRVNVYVDEAFTFACDAELIYKQGIQKDSL
                     IDVEEIKEIVKEDEFIKCKNSALRTVEKTYKTEKELRDKLTEKGFEEDTIKRAIEFLK
                     EYNLLNDEKYAEMYIKDRLRSQGRNKIKYALIRKGVSEDILLDKLSNIDSEDENYTAF
                     KLAEKKYNILKKKESDKYKLSQKLFRFLLSKGYNYDCCNSIVRRLTNNEYME"
     gene            4299..5474
                     /locus_tag="K8M77_001280"
     CDS             4299..5474
                     /locus_tag="K8M77_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBI7085785.1"
                     /translation="MNFNLVDIIIVCSFILPLVVAYKRKFNIIRIKNSIEELGGYISF
                     FLALYLSFIAIKKIDIIERMFSIVVVELNNIIANFNISPKVIIIFIVLALTLVIYFIV
                     KVILKIFSFIIINPILRWLKKAESRRGKGFGKVAALIINIPKSLFYMAVIALVIVILG
                     SNGFLGEKIQGMTLASKAYEVINSNKYYAALNKEYEAFHDEYKDVISKNIDSAVESNK
                     EPKSEKVFESNRNVINLYNGVTLEQGIKSNEAINRKAKELTKNAKSSREKAKRIYTWI
                     SENINYDDNKAENISEKTSEYKSGAIEAFETRKGICFDYSCLYVAMARESGLKVRIVT
                     GEGFNGKEWGPHSWNEVYLPEKNQWITVDPTFGKAGNYFDSKKNSESHRDGKIVGEW"
     gene            complement(5680..6030)
                     /locus_tag="K8M77_001281"
     CDS             complement(5680..6030)
                     /locus_tag="K8M77_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI7085786.1"
                     /translation="MSNFSKGNELYNTRNYSDAINYYKKALNNDECKCHSYYNAGVCY
                     IKLKQYEKAIEMITKALELYQDSKYFFNLAYCYSMINNNSKALRYFNLAWALDNADID
                     CEKAISLILSKISK"
     misc_binding    6242..6454
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6679..9321
                     /locus_tag="K8M77_001282"
     CDS             6679..9321
                     /locus_tag="K8M77_001282"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBI7085787.1"
                     /translation="MDNKNISTTYNPKEFEERLYSNWQEKKYFTPVIDKSKKPYTIIM
                     PPPNITGKLHLGHALDNTLQDMLIRFKRMQEYCTLWLPGQDHASIATEVKVENELLKE
                     GLYKKEMGREAFLEKVWEWTDEYRERIREQLKKMGCSADFTREAFTMDENLSKAVRHV
                     FVKLYKEGLIYQGNRITNWCPKCQTALSDAEIEYEEKEGNFWHIKYPVVGSDEFLEIA
                     TTRPETLLGDSAVAVNPSDERYAHLVGKMLKLPLTDREIPVIADDYVDVEFGTGAVKI
                     TPAHDPNDFEVGKRHNLPQIRVMDDSGVINHLGGKYKGLDRYEARKQMVADLEELGLL
                     VKIKPHTHNVGTHDRCGTVVEPIISKQWYVKMQSLADPAIEVVRNKGTKFVPERFEKT
                     YFNWMENIQDWCISRQLWWGHRIPVFYCKDCGEIMVELEDPTKCCKCGSENIEQDKDV
                     LDTWFSSALWPFSTLGWPDRTDDLEFFYPTSTLVTGYDIIFFWVARMIFSGIHNMGET
                     PFDTVLIHGIIRDAQGRKMSKSLGNGVDPLEVIDEYGADALRFMLVTGNAPGNDIRYI
                     PERVEAARNFANKIWNASRFVMMNLDRELMDKYKDCKEYSLADKWILSRMNSLIKEVT
                     ENMEKYELGIALQKVHDFLWTEFCDYYIELVKPVLYGDDEKAKGVVFNVLYTVLNTGL
                     KLLHPVMPFITEEIYTHLSTETESITIATWPTYDEALNNEKAEKDMTFIMEAIRSLRN
                     LRAEMNVPPSRKAKVMAYASEEAKDAFINGGAYLEKLASASEVTFLDNKDNLDNNLVS
                     VVVKGGELFLPLLDLVDREKELERLNKEKTKLEGEILRVDKKLSNERFVSKAPEAVVN
                     EERAKGVKYKEMLEAVLERIEALQ"
     gene            9621..10943
                     /locus_tag="K8M77_001283"
     CDS             9621..10943
                     /locus_tag="K8M77_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBI7085788.1"
                     /translation="MNYDEAMEYIVTASRFGMNLGLDRIEKVLEFLGNPHKDTKFIHI
                     GGTNGKGSTTAMISSVLREAGFKVGMYTSPYLEEFEERIQINGENINKDDLAILMGDV
                     KKAIDKVMELGYESPTQFEIITALMFYYFSKKKVDYAVLEVGLGGRLDATNVILPEIV
                     VLTSISLDHMNILGNTIEEIAKEKCGIIKGGVPVISYPQEEKALEVIKERCNELGCNL
                     EVVYTKDIRNIIIDKEKHVQKIEVSLEDEILKIDLCLLGDHQVKNFLLALKTLIKLRN
                     NGVNISNEAIKEGFKKVRWIGRMEVLNENPLVVIDGAHNIDGIRSLRNSIDKYFNFKN
                     ITLILGVLGDKQVDEMVEEITRGVDRVVIVEPHSDRAEDIEDLYIKVKKYVKEVFKFK
                     DYKEAYNKAYSLTNDDDLLLICGSLYMVGDMRKAIRNNENTYLRSIKY"
     gene            11054..12613
                     /locus_tag="K8M77_001284"
     CDS             11054..12613
                     /locus_tag="K8M77_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBI7085789.1"
                     /translation="MDKDKEISGLNNLEFKIIVQGILVGIIVGIVIMIYKTIIGFGME
                     GFNKVYSYTRENPKLIIPIFLVLIFLGFIVGIIVKKNPMIGGSGIPQVEGELSGKISV
                     NWLRVFRDKFIGGIICMASGLSLGKEGPSVQIGASIGEGFAKIFKRSDFEKRLLITGG
                     ASSGLAVIFNAPLSGAIFALEEVHRSFSLPVMLAALSASLTGVLVDNLILGNDFCIKI
                     PPTNSLPIQYYWTLLILGAILGATGWIFNKGLLKTQDFYVKTLKKIPIQFKTIIPFVM
                     VGILALTIPEVIDGGDSLIESVIGNNIAIKLLIVILVIKFIFTFFSYSSGVPGGIFFP
                     LLAIGALVGAIFGLLLNKYLGISDSLIVNFIVLAMAAQFASIVKAPITGLMLITEMTG
                     TFKHLLPVAITVTVAYLVSDILNNKPIYESLLEKLLERMNIKFNTGVEKKEIFDFEVK
                     IGSELEGKLIKDVKWPEGSLIITIFRGAEEIIPNGEVKIQSGDVLEVIFSKEKQAQYY
                     DEISKKTYCEI"
     gene            complement(12725..13219)
                     /locus_tag="K8M77_001285"
     CDS             complement(12725..13219)
                     /locus_tag="K8M77_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBI7085790.1"
                     /translation="MNHSADYFIKSLNMIAHPEGGYFKESFVSPSKLAPNTLHINEER
                     LLWTSIYFLLRTGEVSNFHRLKSDEMWYFHAGESLTIYMISPNGELIEKQLGLNIAKG
                     ETPQVLVPKDYIFGSAMNNEGFSLVGCMVAPGFDFKDFELFEREDLLEKYPNYREVIE
                     KLTR"
     gene            13341..13982
                     /locus_tag="K8M77_001286"
     CDS             13341..13982
                     /locus_tag="K8M77_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBI7085791.1"
                     /translation="MSEELNFYTKGEEIANAITHGIGVVLAIAGAVLLIVFSSLSGDI
                     YKIVSFTIFGITLFLLYLGSTLYHSIPNKKVKRFLRIIDHSSIYLLIAGTYTPYVLVC
                     LRDNKKAMVIFIMIWVLTLLGIVFKFICINKFENLSTLLYIFMGWAVIFVVKDVWQRV
                     PHMSVLWLIIGGLFYTLGCIFFVLDRMPYNHAIWHLFVIGGSISHFFSIILYL"
     gene            complement(14032..15432)
                     /locus_tag="K8M77_001287"
     CDS             complement(14032..15432)
                     /locus_tag="K8M77_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBI7085792.1"
                     /translation="MDKKKQFNFSKKQKITIFSLISILILITSVVGFFVFRFYNHSYE
                     GNTDPAKVNTVDEDIKFKEVPGITNILLLSSDARPGEDVSRSDSIMILTIDNINKKLK
                     VTSLMRDMLVKIDGHGEEKLNHAFAYGGPTKTIETIQNNFGIKLNNYVIVDFNAFVKV
                     IDAINGIEVTVKDYELDELNKYILDGGGSEKDLLPSPGTYNLNGYQALSYARIRKVGN
                     GEYERTERQRAVLQIALEKIKDMSKVKIVSLLNELFPYVKTNISLGNAMDYGFTALNV
                     GKKCNFEIEQFRVPLDSISKGGIINNKGWVFVIDKVETSKALQEFIFNDNKNYEPDTS
                     NFDSIIEQYFNDYDIKDDTTHPDYEYVPIINTNNNFENSKNNLNNSSNNNSNDKVEKS
                     SNQGSNENSSNKPSSGSTSTGNNSTEKPNNFKKPNTSQETSKPSENNGDSGNIDSSNT
                     PSIDGNASSENNTEVN"
BASE COUNT         5782 a         1809 c         2560 g         5328 t
ORIGIN      
        1 acttaagttt ctaagcttta gtgatatctt cagagtaata attataaatt cttagcgaaa
       61 tctagtaatt ctcggaatat gtttgtaaga taaaagttac tttgatacat tacctttaga
      121 tttcttctac ttcatctatt tctttagatt tttttagggt aaagtagaat ttaaccccgt
      181 tttctgtatt cataactccg aattcacttt catgagcttg aagtagattt ttaactatgg
      241 atagtccaag tccagtactt ctagagcctc tatttcttga ggtatctccc ctataaaatt
      301 gagtccaaat cttatttata tctgaatcag gaatatgggc accagtattt tcaattgaga
      361 agtagatttt atcttcctca actgacttta tatctattat tatttggttg ttaggttcag
      421 aatattttat agcattggta acaaaattag ttagtacctg ttctatttta aagtcatctc
      481 cttgaacata taaatcgtta gtaggagagt agttttttat tacagttaaa ttatttttat
      541 ttatttcaac agaattcttt actaaaactt cctcagtaag ttctgtaagg gaaaaatcta
      601 ttatatgtaa atcaattttt ccagcttcaa gctttgaaag ttcaagcata tccataacta
      661 atttgttcat tttttctgct tcatctataa ttatatctaa gtaaacccct tgatccttat
      721 gatccactat accgtctttt attccttcag catatccaga tattatacca ataggagttt
      781 taagttcatg ggatgctcca gcaataaagt cttttctaag tttttctagt tctctttctt
      841 tttctatatc ttcttctaac tttttatttt ttatttttaa atcatctaag gcattagata
      901 gatttttaga caaaaagttt aaagttttag ctagatttcc tatttcatct tcacgttcta
      961 tatcacactt ttcagagaag tccatagcac tcattttctt tgcagattta tttaatttca
     1021 ctagaggttt agaaattaag tttgaaaata cataggataa aattaatcca atcactatga
     1081 ctataagaat tatgtattta taaaaatcct ttattaaggc gcttgcttct tcaattgctt
     1141 ggaatgatgt tatggctata ataatagaat cgttcttaga attaagagaa aatggcacca
     1201 tataaacaat atattttgtt gagtattttc tgcttttaaa ttctgtagtt actccagaat
     1261 ttgaatttaa taaggttttt gcaaaatctt ggtcactata taactcatta aaaatatcgt
     1321 ttaataaatc tacagcctct gagtttttat tagctggatc aggaacatat tttggtgtac
     1381 tatctatgga ataaattcct attttagcgt tattttcaag ttcaaaaagg cttaaagccc
     1441 tatataaaga gtctgtattt tttatgtcat aagaatatag agctctaaac tttaaaactg
     1501 cattgcttaa atcattactt tttcttttat aataaaattc ttggaaaaat gtaatttgga
     1561 atatcattgt aaacaaggtt aagaatatta ttataccaaa agttattata aataatcttt
     1621 ttgaaatact ttttttcact atttcacctc aaacttatag ccacttcccc taacagtagc
     1681 tatataatta gctttatcta gtaatttctc tctaagtctt tttatgttcg tatcaactgt
     1741 tcttatatct ccaaaataat catagcccca aacattatct agaattctat cccttgataa
     1801 agctattcct tcatttgaag aaagataaat aagtaaatca tattccttag gagatagtaa
     1861 tagttcttct ccatttagtt ttacttcatg agatagttta tttattgtta atccattaaa
     1921 atctaaaatg tttttatcat ttaatgtacc tgttcttctt aataatgctt tagactttgc
     1981 taataaaacc ttaggactaa aaggtttagt catatagtca tcagctccaa gctcataacc
     2041 taaaagttta tcttcttctt ggcttttagc tgttaataat attataggag tattggaaac
     2101 ttcccttata tttctacaaa cagcaagtcc atctaatttt ggcatcatta tatctaatat
     2161 tgctaaatca actatatttt cagaaaatat cctaagagct tcctcaccat cagaagcttc
     2221 taacacatta aatccctctt ttttgaagta atctcttaca agaaatctta tacgtatttc
     2281 atcttctaca ataagtaccg ttttattcat ataaccacca acctttaaaa aatattgttt
     2341 taacttactt aatattatac aatgaaaact aaggtttata taaataattt taggggagtt
     2401 tttaatgaaa agatggacat ttgatgaaaa taccataatt ttacccaatg ggctaaaagt
     2461 tataacaata aaaaaaaata caaggttagc atctataaat attggtgtta atataggatc
     2521 tttatatgaa gatgaaaaag aattaggcat gagccacttt gttgagcaca tgctttttaa
     2581 aggaacaaaa aatagaagta atgaacaatt aaatagagaa ttagaatttt taggtggaga
     2641 ttataatgca tatacagatt atatttcaac agtatatagc attacttgtc tagatgaaga
     2701 atttgaaaaa ggaatagaac ttttaagcga tatgatttta aattcttcct ttgatgaaaa
     2761 agagatgaaa aaagaaaagg gcgttgtttt atcagagata aaaagtgata aggatgatat
     2821 tgaagattta agtattagca gaacccatga atatgctttt gataaatcag cacttagaaa
     2881 ttctattgca ggaactgaag aacatgttaa gggatttaaa agaaagcagg tttatgattt
     2941 ttataaaaaa tattatactc ctgataattg tgttattgta actgtaagtg cctttagtca
     3001 tgaacaaatg caaaaaataa taacagactt atttggaaaa tgggaaggaa aatctcataa
     3061 aaaggctaaa ataataaaag aagaaaataa aaacatagta aaaacaactt acaaatctca
     3121 aatagagcaa ggaacagtta cttatcttta tacctttaaa gaagtttgtg aaaaagataa
     3181 acttccatta aagatattaa gttataaact agctgaaagt tcaaactcaa tattatttag
     3241 agagcttaga gaagagcgtg gattagctta tgatgtatat agtcaaatgg atttagacga
     3301 aaatgtaaat acaatgaata tttttacctc agttagagaa gaatccatag atgaggtaat
     3361 agaagtaata gacaaagcta tattagacat aaaaaataga gatataaact ttgatgaaga
     3421 tatgctttgt atgatgaaaa aaactcataa gacaggagtg gtttctacct tagaagattg
     3481 cagcagttta tgcagttatg tattagttca aagcttagcg ggaaaagata taactgagtt
     3541 tataaatagc atggaagaat tagaaacttt aactggggaa gatatctata gggtttgtaa
     3601 taaatatttg aataagccta ctatacacat attaaaacca atggagtaga taaaatggga
     3661 aaaataacga gtatagaagt tcaaaagaga aatgtaaata gagtaaatgt ttatgtggat
     3721 gaagccttta cctttgcttg tgatgctgaa cttatatata aacaaggaat acaaaaggat
     3781 tctttaatag atgttgaaga gatcaaggaa attgtaaaag aagatgaatt tataaaatgt
     3841 aaaaattctg cactaagaac agttgagaaa acctataaaa cagaaaagga attaagagat
     3901 aaacttactg aaaaaggttt tgaagaggat acaattaaaa gagccataga atttttaaag
     3961 gagtacaatc ttttaaatga tgaaaaatat gcagagatgt atataaagga taggcttaga
     4021 agtcagggaa gaaataaaat aaaatatgct cttataagaa agggcgttag tgaagatata
     4081 cttttagata aattatctaa tattgattca gaagatgaaa attatactgc ttttaagtta
     4141 gcagaaaaga aatataatat actaaagaaa aaagaaagtg ataaatataa attatctcaa
     4201 aaattattta gatttctttt atctaaagga tataattacg actgctgtaa tagcatagtt
     4261 agaagattaa caaataatga atatatggag tagataaaat gaattttaac ttagtagata
     4321 taattatagt ttgttctttt atactaccat tagtagttgc ttacaaaaga aagtttaata
     4381 ttattagaat aaaaaatagt atagaggaat taggaggata tatatccttt tttcttgctt
     4441 tatacttaag ttttatagct ataaaaaaga tagatataat agagaggatg ttttcaattg
     4501 tagttgttga actaaataat attatagcta actttaatat ttcaccaaag gttataataa
     4561 tattcatagt attagctctt acccttgtaa tttattttat agtaaaagtt atacttaaaa
     4621 tatttagttt tattattata aatccaatac ttagatggtt aaaaaaagca gagtctagaa
     4681 ggggtaaggg atttggtaaa gttgcagcct taataataaa tataccaaag tctctttttt
     4741 atatggcagt aattgcttta gtcatagtta ttctaggaag caatggtttc ttaggagaga
     4801 aaatacaagg catgacttta gcttcaaagg cttatgaggt tataaatagt aataagtact
     4861 atgctgcttt aaataaagaa tatgaagctt ttcatgatga atataaagat gttattagca
     4921 aaaacataga ttctgcagtt gaaagtaata aagagccaaa gagtgaaaaa gtgtttgaaa
     4981 gtaatagaaa tgttataaat ctttataatg gtgtaacttt agaacaaggt ataaaatcaa
     5041 acgaagctat taatagaaaa gctaaggaac ttactaaaaa tgcaaaaagt agtagagaaa
     5101 aagctaaaag aatatatact tggataagtg aaaatattaa ttatgatgat aataaagctg
     5161 aaaatataag cgagaaaact tctgagtata agtctggtgc tattgaagcc tttgaaacta
     5221 gaaaaggaat atgctttgat tattcttgtc tttatgttgc tatggcaaga gaatcgggac
     5281 ttaaagttag aattgtaact ggagaaggat tcaatggaaa ggaatgggga ccacattctt
     5341 ggaatgaggt ctatttacca gaaaaaaatc aatggataac tgttgatcct acctttggta
     5401 aagctggaaa ctattttgat agcaaaaaaa atagtgaatc acacagagat ggaaaaatag
     5461 ttggtgaatg gtaagataat aagttatatg aaatacaata aaacttacaa atatattttg
     5521 aagaaaataa aatttcttct aatactattt gtaagtttta ttttggtttt agggagaatt
     5581 agatttttct atatttcttt taatgaaatt ttgcttatgt aattttaatt ataaattaga
     5641 ataggtattg aaagttaagt gagctatata tattatgatt tattttgata tttttgataa
     5701 aattaaactt atagcttttt cacaatcaat atctgcatta tctaatgccc aggctaagtt
     5761 gaaatatctt aaagccttag aattattatt tatcattgaa taacaatagg ctaagttaaa
     5821 aaaatatttt gagtcttggt atagctctaa agctttagtt atcatttcaa tagctttttc
     5881 atattgcttt agttttatgt agcaaactcc agcattatag taactgtggc atttacactc
     5941 atcgttattt agagcttttt tatagtaatt tatagcatca gaataatttc tagtattgta
     6001 taattcatta cctttagaaa agttgctcat ttttgacctc ctaaataata tctattttaa
     6061 ttattaactt attttggaat tatattcata ggttgagaat attatataat ttgattaagt
     6121 cgtaagatta tagaatcata tttaaagact aaaaatatta taaatttata tcatatttga
     6181 ttagagttac ttgacatagg ttaaaataat ggtataagat atatctataa tttaaatgat
     6241 gatacaaaca gtgataaaga ggagtaaaga taggaagaac tttaagagag gaaatccttg
     6301 ggtgtaagat ttctaagttt gatatcttga aggtagcttt tgagtttctt tggtgaaatt
     6361 attaaagtaa tcaaagacgg caatttcagt cgttagtttt agagagccct tttgtgaggg
     6421 aaaaaaggtg gtaccgcgag ttttcgtcct tttatggatg agggcttttt ttattgccta
     6481 aaattacttt taaataaagg aatgttttaa atcagtcttt gtttttagat ataacataaa
     6541 tataaatggc tagctaaaat aaatattaat ttaaaataga ccataaataa aaattgttaa
     6601 aaggcatttc aagatagttt ttggaatgtt ttatataaat acataaaatt gtgtttaagt
     6661 tttaaggagg taagtctaat ggataataag aatatttcta caacgtataa tcctaaagag
     6721 tttgaagaaa gactttatag caattggcaa gaaaagaagt attttactcc tgtaatagat
     6781 aagagtaaaa aaccttacac tataataatg ccaccaccaa atataactgg taaattacac
     6841 ttaggtcatg cacttgataa cacacttcaa gatatgctta taagatttaa gagaatgcaa
     6901 gaatattgta ctttatggtt accaggacag gaccatgcta gtatagcaac tgaggttaaa
     6961 gttgaaaatg aacttttaaa agaaggtctt tataagaaag aaatgggaag agaagctttc
     7021 ttagaaaaag tttgggaatg gacagatgaa tatagagaaa gaataagaga gcagcttaag
     7081 aaaatgggat gctcagctga ctttacaaga gaagctttca caatggatga aaacttaagt
     7141 aaggcagtaa gacacgtatt cgttaaatta tataaagaag gtttaatata ccaaggaaac
     7201 agaataacta actggtgtcc taaatgtcaa actgctttat cagatgctga gatagagtat
     7261 gaagaaaaag aaggaaactt ctggcacatt aaatacccag tagttggttc agatgaattc
     7321 ttagaaatag ctacaactag acctgaaact ttacttggag atagtgccgt agcagttaac
     7381 ccaagtgatg aaagatatgc tcacttagta ggtaaaatgt taaaattacc tttaactgat
     7441 agagaaatac ctgtaatcgc tgatgactat gttgacgtag aatttggaac aggagcagtt
     7501 aaaataactc cagctcacga tcctaatgac tttgaagttg gtaagagaca taacttacca
     7561 caaataagag ttatggatga tagtggtgta ataaaccacc taggtggaaa atacaaaggc
     7621 ttagatagat atgaggctag aaaacaaatg gttgctgact tagaagagtt aggattatta
     7681 gttaaaataa aacctcatac tcacaatgtt ggtactcatg atagatgtgg tacagttgta
     7741 gagccaatca tatcaaaaca atggtatgta aaaatgcaat cattagcaga tcctgctata
     7801 gaggttgtta gaaacaaagg aactaaattc gttccagaaa gatttgaaaa gacttacttc
     7861 aactggatgg aaaacattca agattggtgt atttcaagac aattatggtg gggacacaga
     7921 ataccagtat tctactgtaa agattgtgga gaaatcatgg ttgaattaga agatccaact
     7981 aagtgctgta agtgtggatc agagaacata gaacaagata aagacgttct agatacttgg
     8041 tttagttcag ctctatggcc tttctcaact ttaggatggc cagatagaac tgatgattta
     8101 gagttcttct atccaacatc aacattagtt acaggatacg atataatatt cttctgggtt
     8161 gctagaatga tattctcagg tatccacaac atgggagaaa ctccatttga tacagtatta
     8221 attcacggta tcataagaga tgctcaagga agaaaaatga gtaaatcttt aggaaatggt
     8281 gttgatccat tagaagttat agatgaatat ggtgctgatg cattaagatt catgttagta
     8341 acaggaaatg ctccaggaaa tgatataaga tatattcctg aaagagttga agctgcaaga
     8401 aactttgcta ataagatttg gaatgcttca agattcgtta tgatgaactt agatagagag
     8461 cttatggata aatacaaaga ctgcaaagag tatagcttag cagataagtg gatattatca
     8521 agaatgaact cattaatcaa agaagttaca gaaaacatgg aaaaatatga gttaggaata
     8581 gctcttcaaa aagtacatga tttcttatgg acagagttct gtgattacta tatagaatta
     8641 gttaaaccag tattatatgg tgatgatgaa aaagctaagg gagtagtatt taacgtactt
     8701 tacactgtat taaatacagg attaaaatta ttacacccag taatgccttt cataactgag
     8761 gaaatatata ctcacttaag cacagaaact gagtcaatta caatagctac ttggccaaca
     8821 tatgatgaag ctttaaataa cgaaaaagct gagaaggaca tgacattcat aatggaagct
     8881 ataagatcat taagaaactt aagagctgaa atgaatgtac caccttcaag aaaagctaag
     8941 gttatggctt atgcttcaga ggaggctaaa gatgcattta taaatggtgg agcttactta
     9001 gaaaagcttg catctgcatc agaagttact ttcttagata ataaggataa cttagacaat
     9061 aacttagtat cagtagttgt taagggtgga gaattattct taccattact tgacttagtt
     9121 gatagagaaa aagagttaga aagattaaat aaagagaaaa ctaaacttga gggagaaatc
     9181 ttaagagttg ataagaaact ttctaatgaa agatttgtaa gcaaagcacc agaggctgtt
     9241 gttaatgaag aaagagcaaa aggtgttaaa tataaagaaa tgttagaggc tgttctagaa
     9301 agaatagaag ctttacaata atatttagcg tattattttt tactagactc caagaattac
     9361 ttaatttcgt aaagtatttc tattatctat tccagaataa tagctaagac ttctaaactt
     9421 gcttcgcttc gaacaaaaga agtctcttaa cgctattatt cttacataga taatgaaata
     9481 ctagttactc attaagatat tctttacgtt ctagttaaaa aataaatatt tgctaaattt
     9541 agagagtgtg ttgataatta actttattat gttagtttga tacacactct attattatat
     9601 ttaggagggc tataggaatg atgaattatg atgaagctat ggagtatatt gttactgcat
     9661 caaggtttgg aatgaattta ggattagata gaatagaaaa agttttggaa tttttaggga
     9721 atccgcataa ggatactaag tttatacata ttgggggaac aaatggtaaa ggctcaacta
     9781 ctgctatgat aagttctgtt ttaagggaag ctggctttaa ggttggaatg tacacatcac
     9841 cttatttaga ggaatttgaa gagagaatac agattaatgg tgagaatata aataaagacg
     9901 atttagcaat tcttatggga gatgttaaaa aggctataga taaagttatg gagcttggct
     9961 atgaaagtcc aactcagttt gaaataataa ctgctttaat gttttattat ttttcaaaaa
    10021 agaaagttga ttatgctgtt ttagaagttg gtttaggggg aagattagat gcaaccaatg
    10081 tgatccttcc tgagatagtt gtacttactt caataagcct agatcatatg aatattttag
    10141 gaaataccat agaggaaatt gcaaaggaaa aatgtggaat aataaagggt ggcgtacctg
    10201 taatatctta tccacaagaa gaaaaggcct tagaagtaat aaaagagaga tgtaatgaat
    10261 taggttgtaa tttagaggtt gtttatacta aagacataag aaatataatt atagataaag
    10321 aaaaacatgt tcagaaaata gaagtctcac tagaagatga aattttaaaa atagatttat
    10381 gtttacttgg agatcatcaa gtaaagaatt tcttattggc attaaaaacc ttaattaaat
    10441 tgagaaataa tggagtaaac ataagtaatg aagctattaa ggaagggttt aaaaaagtaa
    10501 gatggattgg aagaatggaa gtattaaatg aaaatccatt agttgttata gatggagctc
    10561 acaatattga tggaataaga agtttaagaa atagtataga taaatatttt aattttaaaa
    10621 atataacatt aatattaggg gttctaggtg ataaacaagt agatgaaatg gtggaagaaa
    10681 taactagagg agtagatagg gtagttatag ttgaacctca tagtgatagg gcagaggata
    10741 ttgaagattt atatattaaa gtaaaaaaat atgtaaaaga agtttttaag tttaaggatt
    10801 ataaagaggc ctataataag gcgtattctc ttactaatga tgatgattta ttattaattt
    10861 gtggttcttt atacatggtc ggagatatga gaaaggccat aagaaataat gagaatacat
    10921 acttaagaag tataaagtat tagacttaag tagataagtt gtattcaact ggataaatga
    10981 gaaaattaaa aattgaactg ttgttaacaa aaggtatata atttattcat aaaatattat
    11041 ggatgtgatt aatatggata aggataaaga gataagtgga ttaaataatt tagagtttaa
    11101 aataatagta caaggaatat tagtaggtat aattgttgga attgtaataa tgatttataa
    11161 gaccataata ggatttggaa tggaaggctt taataaggta tattcatata caagagaaaa
    11221 tccaaagctt ataattccta tatttttagt tcttatattt ttaggattta tagttggaat
    11281 catagttaaa aagaatccta tgataggtgg aagtggtatt ccacaggttg aaggggagtt
    11341 aagtggaaaa ataagtgtaa actggctaag agtatttaga gataagttta ttggcggaat
    11401 aatatgtatg gcatctggcc tttcattagg taaagagggt ccttcagttc aaattggagc
    11461 ttcaatagga gaaggctttg ctaaaatatt taaaagaagt gactttgaaa agagattatt
    11521 aataacaggg ggagcaagtt caggtcttgc tgtaatattt aatgcccctt tatcaggagc
    11581 tatatttgct cttgaagaag tacatagaag tttttctttg ccagttatgt tagctgcact
    11641 ttcagcttct cttacaggag ttttggtgga taatcttatt ttaggaaatg atttttgcat
    11701 aaaaatacca cctacaaatt cattgccaat acaatactat tggacattat taatattagg
    11761 agccatatta ggagcaacag gatggatttt taataaagga cttttaaaaa cacaggattt
    11821 ttatgtgaag actttaaaaa aaattcctat tcaatttaaa acaataatac cttttgttat
    11881 ggttggaatt ttagccttaa caattccaga agtaattgac gggggagact ctttaattga
    11941 aagtgtaata ggaaataata ttgcaataaa acttttaata gtaattttag taataaaatt
    12001 tatatttaca ttctttagtt attcatcagg agtgccagga ggaatattct ttcctttatt
    12061 agctataggt gctttagtag gagcaatatt tggattactt ttaaataaat atttaggaat
    12121 aagtgattcc ttaatagtca actttatagt gctagctatg gcagcacaat ttgcatcaat
    12181 agttaaggct cctattactg gattaatgct tataactgag atgacaggaa cttttaaaca
    12241 cttattgcca gttgctataa cagttactgt ggcatattta gtttctgata tacttaataa
    12301 taaacctatt tatgaaagtt tattagagaa attattagaa agaatgaata ttaagtttaa
    12361 tactggcgta gagaaaaaag aaatttttga ttttgaagta aagattggtt ctgaattaga
    12421 aggaaaactt ataaaagatg tgaagtggcc tgagggtagt ttaataataa ctatctttag
    12481 aggagcagag gaaataatac caaatggaga ggttaaaatt caatctggag atgtgttaga
    12541 agtaatattt agtaaggaaa aacaagctca gtactatgat gaaatatcaa aaaaaacata
    12601 ttgtgaaatt taattgataa aatacattaa attaaaagct aataaaaaac ttacaaataa
    12661 ttcttaaaga aatagggtga ttctttaagt aaatttgtaa gttttttatt tttataaaag
    12721 ttttttatct agttaatttt tctattactt ctctgtaatt tggatatttt tcaagcaaat
    12781 cttctctttc aaataactca aaatctttaa aatcaaagcc aggtgctacc atgcaaccaa
    12841 ctagggaaaa gccttcatta ttcatagcag aaccaaatat ataatcctta ggaactaaaa
    12901 cttgaggagt ttctcctttt gctatgttta atcctagttg tttttctata agttcgccat
    12961 tagggcttat catataaatt gttaaggatt ctccagcatg gaaataccac atttcatcag
    13021 attttaacct atgaaaattt gaaacttctc cagttcttaa taagaaatat atgctagtcc
    13081 aaagtaaacg ctcttcattt atatgtaaag tatttggagc aagtttgcta ggtgaaacaa
    13141 aggattcttt aaaatatcca ccttcaggat gagctatcat atttaatgat tttataaaat
    13201 agtcagcaga atgattcata ctaaaaacca cctttaaaat aatttttcta aacatgttta
    13261 atcataaagt ataaatgata taattacaaa tataaattta aaatatgcat ataataaaat
    13321 gtgaaagggg gatggtaatt gtgagtgaag aattaaattt ttatactaaa ggagaagaaa
    13381 ttgctaatgc aataactcat ggaattggag tagtattagc tatagcagga gctgttttac
    13441 ttatagtttt ctcatcttta agtggagata tttataagat agtaagtttc actatttttg
    13501 gaataactct atttttactc tatttaggat caacattata tcatagtatt ccaaataaaa
    13561 aagtaaagag gtttttaaga ataattgacc attcatcaat ttatctttta atagcaggaa
    13621 catatacacc ttatgtatta gtatgtttaa gggataataa aaaagctatg gttatattta
    13681 ttatgatttg ggtgctaacc ctattaggaa tagtatttaa atttatttgt ataaataagt
    13741 ttgagaatct atcaacctta ttatatatat ttatggggtg ggcagttatc tttgtagtca
    13801 aagatgtatg gcaaagagta cctcatatgt ctgtactttg gcttattata ggtggattat
    13861 tttatacctt aggatgtata ttttttgttt tagatagaat gccatacaat catgctattt
    13921 ggcatttatt tgtaatagga ggaagtataa gccacttttt ttctataata ttatatttat
    13981 aataaaaaag ctatatggaa tctaataacc ttagattcca tatagctttt attaatttac
    14041 ttccgtatta ttctcagaag aagcatttcc atcaatagag ggagtgttag aggaatctat
    14101 atttccacta tctccattat tttcagatgg ttttgaagtt tcttgagaag tatttggttt
    14161 tttaaaatta ttaggttttt ctgtagaatt attaccagta gaagtgcttc cactacttgg
    14221 tttattagaa gaattttcat tacttccttg gttagatgat ttttctactt tatcattaga
    14281 attattatta ctgctattat ttaaattgtt tttagaattt tcaaagttgt tattagtgtt
    14341 tataataggt acatattcat aatctggatg agtagtatca tctttaatat catagtcatt
    14401 aaagtattgt tctattatag agtcaaaatt actagtatca ggctcataat ttttattgtc
    14461 attaaagatg aactcttgaa gagcttttga tgtttctacc ttatcaataa caaagaccca
    14521 acccttatta ttaatgattc caccttttga aatactatct agaggaactc taaattgctc
    14581 tatttcaaag ttacactttt tacctacatt taaagctgta aatccataat ccatagcatt
    14641 tcctaaagaa atattagttt taacatatgg gaataattca ttaagcagag aaacaatttt
    14701 aacttttgac atatctttaa ttttttctaa agcaatttga agaacagctc tctgtctttc
    14761 agttctttca tattcaccat taccaacttt tctaattcta gcataagata aagcttgata
    14821 accattaaga ttataagttc caggtgaagg aagcaaatct ttttcagaac cgccaccatc
    14881 taaaatatat ttattaagtt catcaagttc ataatcttta acggttactt caattccgtt
    14941 tatagcatct ataaccttaa caaaagcatt aaaatctaca attacataat tatttaattt
    15001 tattccgaaa ttattttgaa tagtttcaat tgttttagta ggaccaccat aagcaaaagc
    15061 atgatttaat ttttcttctc catgaccatc tatttttact aacatatctc tcattaaaga
    15121 agttactttt aattttttat tgatattatc tattgttaag atcattatgg aatctgatct
    15181 agatacatct tctccaggtc tagcatcact actaagcaaa agaatatttg ttattccggg
    15241 aacttcttta aacttaatat cttcatccac agtattaact ttagcagggt ccgtatttcc
    15301 ttcgtaggag tgattgtaaa atctaaaaac aaaaaagcct actacagatg taattaatat
    15361 aaggatagat attaaagaaa atatagttat tttttgtttt ttactaaaat taaattgttt
    15421 ctttttatcc ataagtactc ctttttatga tttgtttaaa cgttttaatg agtagctaa
//
LOCUS       DAEVGI010000049        15432 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEVGI010000049 DAEVGI010000000
VERSION     DAEVGI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 15432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15432
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.049"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(300..646)
                     /gene="rnpB"
                     /locus_tag="K8M77_001288"
     ncRNA           complement(300..646)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="K8M77_001288"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(694..951)
                     /locus_tag="K8M77_001289"
     CDS             complement(694..951)
                     /locus_tag="K8M77_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085793.1"
                     /translation="MWSNFGNRFNGDCFRDLRRDFNKLMRNFKKNACKRYLVNNCYFK
                     NALKWGAIGGILTFLIISQIGIPLAIVCIGIVAILVICNKW"
     gene            complement(1109..1798)
                     /locus_tag="K8M77_001290"
     CDS             complement(1109..1798)
                     /locus_tag="K8M77_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI7085794.1"
                     /translation="MDRYNVIDNKNNREIVLLKSFPCVWGKCAFCDYIEDNSKNTEEI
                     IKLNKEVLSNVKGIYGVLEVINSGSVFELPKETLEEIKRIVIEKNIKKLFFEAHWCYR
                     NRLKEIEEYFGVPIIFKTGIETFDDYFRNDILNKNARFNDVEEVKKYFKSICLMVGIK
                     GQTKDMIKRDMGILLNNFKYGTVNIWTENTTSFKRDEELIKWFEKEYSFLKDNKTIEV
                     LFENTDFGVGD"
     gene            complement(1816..2373)
                     /locus_tag="K8M77_001291"
     CDS             complement(1816..2373)
                     /locus_tag="K8M77_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI7085795.1"
                     /translation="MRNSKSLKLTIMGIGIALNIIGAFIALNLRLPIYLDSIGTIMVG
                     FVLGPVYGMITGVLGSVVSGITFDIYSLYFAPVQIFTGFFAGYFYEKGLMKGKKLFLS
                     VFVMTLFVSFTGACLTAYIFGGITSSGSSYIIVILNNLGVNPVVSAFITQFLTDYCDK
                     LVAVLIMLQVVVRLPKNILLRVQNN"
     gene            complement(2392..3372)
                     /locus_tag="K8M77_001292"
     CDS             complement(2392..3372)
                     /locus_tag="K8M77_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBI7085796.1"
                     /translation="MDKRKVIIDCDPGIDDALAIILALKSKEIEVVGITTVSGNVESV
                     QGAKNALKALKLLGRLDIPVYLGESKPVKRELVTAQDTHGEDGLGETFLEEVSSEYIR
                     ENGVDFILNTLKNQENVSIIALGPLTNLYRAIEKDSETFHRVKEIVSMGGAYKSHGNC
                     SPVAEFNYWVDPHGAREFLKKFNGEFTMVGLDVTRKIVLTPNLREMIHQFNDEIGNFI
                     YDITRFYVDFHWEQERTLGCVINDPLAVEFFINRDICEGFKAYVDIACEDISMGQSVV
                     DVADFYKRRKNVFVLDKVNSKKFMVSFLNKIFPSYKEDIENILNNPKYGI"
     regulatory      complement(3699..3743)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(3883..4671)
                     /locus_tag="K8M77_001293"
     CDS             complement(3883..4671)
                     /locus_tag="K8M77_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBI7085797.1"
                     /translation="MKLNDIINIIEDIAPVNLKEGFDNVGLMVGDREKNITKILLALD
                     CTEEVIKEAKEMGAELILTHHPLLFRKPSTITTDTLLGRKIISLIKNDINLYSAHTNW
                     DSVKGGLNDTLVEILGFNKGIIMDKSPVNSQAGIGRVVELTKEMTVLEIINLIKSSLG
                     IKNLRYAGDLNEVIKKIAIVNGSGQDFFGDAKKLGADLIITGDTTYHFVSDYKEMGLN
                     ILDIGHFNSEWPVLIKVSEKVKERLDSDVEFIVSKEAKDPFEFI"
     gene            complement(4671..5360)
                     /locus_tag="K8M77_001294"
     CDS             complement(4671..5360)
                     /locus_tag="K8M77_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBI7085798.1"
                     /translation="MELSKRLKRIAEHVDKCESVADIGTDHGYIPIYLVKEGICKKAI
                     ASDINKGPIEKAKVNVAFEGVSNKVKCLLGPGLNPLKVGEVNGVILAGMGGNLTRDIL
                     LADMDKVKKYDFIILQPAQNPEVLREFLYKNDYEIIDEDLIKDEGRFYELFKVKYNEN
                     SEKLVFEDELEYEVSPLLREKGHPLFKEFIEEKINRCETILSFIKEDTEAAKKRKSDL
                     EEKINKLKGML"
     gene            complement(5374..6261)
                     /locus_tag="K8M77_001295"
     CDS             complement(5374..6261)
                     /locus_tag="K8M77_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBI7085799.1"
                     /translation="MTKFKAKRGHGVFEVFAYLLLGNALLIILSNIVNGYLERNLVIV
                     SSFVYSIVCIYYILLDLSLVYEVNDDYIEINCFKGLKKFRINFKDVNGFLEQDKVING
                     FKLSGFGKHKYCFGRCVVHNVGIARMFVTSSKRVIYIHTEDISYGLSPDEFEKFKELI
                     VSKGIKEESFKVKVNNARAIIRDKGFYIPFIITSLLILGIILIPSILYLSGAMPDKMP
                     IDFSAHFIPCTIGSAKDFVGRQIIYGIMNMILLVCIYYASHFCAKYDKKLAQRYIYLS
                     LIISLVFLVSQIQTLVNYL"
     gene            complement(6497..7618)
                     /gene="rpoD"
                     /locus_tag="K8M77_001296"
     CDS             complement(6497..7618)
                     /gene="rpoD"
                     /locus_tag="K8M77_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBI7085800.1"
                     /translation="MAEGGNKMKAKTTKAKKGEGKEKVDKMVLVKRLIDKGKKSGSLT
                     YKEIMDELDEIELNPEQIEKIYEVLESMGIEVISEIEQEEEEEEELDLSVPEGIAIDD
                     PVRMYLKEIGKVPLLSSEDEIELAKKIEEGSNYAKKKLAEANLRLVVSIAKRYVGRGM
                     LFLDLIQEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMV
                     ETINKLIRIQRQLVQELGRDPLPEELSKQMDMPVDKVREILKIAQEPVSLETPIGEEE
                     DSHLGDFIPDDDAPAPAEAAAFTMLKEQLINVLDTLTPREEKVLRLRFGLDDGRARTL
                     EEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDYLD"
     gene            complement(7624..9411)
                     /locus_tag="K8M77_001297"
     CDS             complement(7624..9411)
                     /locus_tag="K8M77_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI7085801.1"
                     /translation="MRISEEIIEKVKEQNDIVDVVSDVVRLKRAGRNFSGLCPFHNEK
                     SPSFSVSPDKQIFKCFGCGEAGNVISFVMKTKNLNFVDAVKELADRANIIIPIEDGKQ
                     SESQKKKQVLYNINKQAARYYFSNLQNNKEAKEYFLRRGIKENILKSFGLGYAKEGWQ
                     NTINHLRKQGFSNEAIEAAGLGIKSEKGTFYDRFRNRVIFPVFDYKGSVIGFGGRVLD
                     DSKPKYLNSPETLVFHKGTNLYALNFAIKHGLQERTLIIVEGYMDCISLHQYGITNVV
                     ASLGTALTDNQARLLKRYVDRVIISYDADIAGQMATIRGLEILQNAGLDVRVLTVPKG
                     KDPDEFIRANGKESFIKLIQSAKKLIDYKLHRARIGIDFSDNNGLIKYAERVVNILSD
                     LNPIEKDVYIKRISEETSIKEQAFYDLLNKNLINSNKNHENVNDKEEYGTKLYVEPPY
                     VKCERSVLKLLCMEENHSLVKKYLEDDDLISPNIKSLEKIINDSIEEIGFNNLGKHVE
                     AKCEDPKVLKEWLEVKELLILDHSSNIEVLVRDYINLIRKYKLEQEQTTVLKELKRCE
                     QNNMFKESLDLVKRAKEISDKLKELERSK"
     gene            complement(9580..10608)
                     /locus_tag="K8M77_001298"
     CDS             complement(9580..10608)
                     /locus_tag="K8M77_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HBI7085802.1"
                     /translation="MKIRENIENFERIKLNKVAKFSDESVGRERLEEPDEIRTCFMVD
                     RDRIIHSKSFRRLKRKTQVFIRTYGDHYRTRLVHTLEVSQVARTIGVALSLNEYLIEA
                     IALGHDLGHAAFAHIGEDVLNDFLPGGFKHNEQSVRVAKKIEKNGLGLNLTKEVLDGI
                     LNHSGFSNVSKVAGTFEGQVVRFADKIAYVNHDIDDSIRAGILKEEDLPKNIIEILGA
                     SGSERIDTLVKDCVFNTIDNIDKGEPRVSLSKDIGDAFVQLRKFLFDNIYLGKYLEDE
                     RKKAEFVLEKVIEYYYNNWGEMPELYRNICEEEGIHRGVTDYIAGMTDDYCTNEFNKI
                     YIPKFVMY"
     gene            10808..11887
                     /locus_tag="K8M77_001299"
     CDS             10808..11887
                     /locus_tag="K8M77_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HBI7085803.1"
                     /translation="MGKHSKKFNEADEIFNAVESIVLPMYNLENYSIENIKFKNTDKN
                     RAVYKLIDDINNPKNTFCLKKVYYDEGTLLFIYSVMEWFARNEIKLPKMLPSKFNGRF
                     VKANNMLFMLCPWVKGEKCNFDNLQHILLSIENLAKMHNCSRNFKAIEGSLIKTGFDS
                     LYISTLKHFNKILSSFNTATKMKHKDKFSSIFLDVFDENIYLAKEALLVSGAINNKNL
                     SRSLCHGDYVNKNILIDNTDVWVIDFDKASLNYSMYDLCYFMRRLLKRSNTNWDIDLT
                     RKIIKTYNSIAPLTEDDFKYVFSYLAFPQKYWRLSKDYYNNIKKCNKSMFVESLKEVA
                     LDTYAQVRFVEELRTLLKKDFLLTL"
     gene            complement(11964..12647)
                     /locus_tag="K8M77_001300"
     CDS             complement(11964..12647)
                     /locus_tag="K8M77_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003360624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085804.1"
                     /translation="MDLIKLRSVVRTYGKGENIVHALRNINLSIKSGEFVAIMGPSGS
                     GKTTLLNILGCLDSADSGSYELEGREITDTSEKELAKIRNANLGFVVQSFELLNDYTV
                     YENIKIPLEYSDKKIKNKKEKVMGILKELDLLDKFNKYPTELSGGQCQRIAIARALIN
                     EPKIILADEPTGALDKRTSNEVMNIFKDINKGGTTIILVTHDINIASQCHRIINIEDG
                     LIVNDIKNS"
     gene            complement(12651..13892)
                     /locus_tag="K8M77_001301"
     CDS             complement(12651..13892)
                     /locus_tag="K8M77_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085805.1"
                     /translation="MNNLKYSIRELKNSLFFKIVLILQLMLVIILTYRIIESKNYQNE
                     KLSSMNEIIGNKTIYQIALKYDSIDEFLEDQKDENKFSNFALDIDKNFNMVTTVLGEI
                     KLPYFNGIEKFLDNDFKESEQGEEMKSVYSLQCSPNFFDTFNIGLSEGSLKDFEKFSQ
                     LNSVEWENKYIPIVLGNAYKGIFNIGDIIENKYFNYKVVGFIEENRFFLDKGVYDLSR
                     VKNLNTFIITPMPKDIINANVSNAFLVYNSDPREFNEVKQEVESLAKNNKVKISVYDP
                     NENIDTFTKEIKYNINLNLLLIYIVGIFAIGGFVVIFCNRVLDRRKEFSIHIMHGATI
                     SDICFRVIIEQFILVFISTIIAYSYLMLHSVNFVTEVVQFQYGTFAKSISIIIGAIIL
                     VSMIPIYYIKKYKLNYLIKGE"
     gene            complement(13889..14983)
                     /locus_tag="K8M77_001302"
     CDS             complement(13889..14983)
                     /locus_tag="K8M77_001302"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014717.1,HMM:NF024116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085806.1"
                     /translation="MISFKKYKKLTYIITTFTLIVFTFSIVLSILVLSYKNKEEVNNI
                     SNLTEKYFKFNDSNNVTFREVCEIVDNYNNSQIYLEYEPLPKYLNNDTIFGRGIYFKN
                     GFQKSFPILEGRDFTKEEIENGEKVALVGVNFKDEVFEENNERFFTIGEEKFKVIGIL
                     GSSERQTGYDNTFLFNLKSSDEFSDTRSSWKINIENNEALKNILNDYKKLAVNKNTSF
                     SLEDIKKDFNLKEALNEYKDFIDILFLVFIIGILNLIIAIYYWIKKSIKEIGVRKAYG
                     ASNGKIVIHILKQYLISICIALIMGVSLHLLLKRLLIYMFPEFTFDVYYQSIIFITII
                     FMFIGLIAVLIPLFKAKKVKPINIMKGKLK"
     gene            complement(15042..15413)
                     /locus_tag="K8M77_001303"
     CDS             complement(15042..15413)
                     /locus_tag="K8M77_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085807.1"
                     /translation="MIKKSIILGASMVMLSGGILVSAAQGTTVSGASKTTSYHGWVEG
                     SRNSSNCYGATTTVIDSGNEKAYAYVEIVNADGYRIGNGASNTGNTRAHSSTITRTGG
                     RNAYGSVGTSTGSSRTFARLY"
BASE COUNT         5130 a         2798 c         1452 g         6052 t
ORIGIN      
        1 aattttcttt catcatatcc taaaaataaa acaaaaaata taacttttat aatctcaaat
       61 acttttctca tttgtttcaa ctcctttaaa ttcttacaaa atacttatga atttattagt
      121 tatgaattca tatttttcta aattacgaat tcctttcaaa tttataatct cctctattag
      181 atatatccaa taaaattaaa ttaatactgt aagattaaag ttaataagga aagaaactat
      241 cagcatattt ttttgtaaaa taaaaaaaac acttaggtat tataacctag tgttttataa
      301 atacgagata agtctataag ccgagttctg tatttgacaa tcatctatct aggcctaccg
      361 ttgccgatag gctcaagcga cacacccgag gacgggacgg gcagccccta tatgtcctcc
      421 tatttggtct tgctccagat ggggtttaca tagcagtata gtcacctata cactggtgag
      481 ctcttacctc acctttccac ccttacctag gtaaatctag gcggtttatt tctgttgcac
      541 tatccttaga gtcacctcca ctggacgtta tccagcatcc tgccctatgg agctcggact
      601 ttcctctcct agttctaaga ctagcagcga ttgtctgact tactcgcaat attaatatta
      661 acatatatac ttattttttt caaattattt ttactaccat ttattacaaa tcacaagtat
      721 tgctactatt ccaatacaaa caattgctag aggaattcct atttgactta ttataaggaa
      781 ggttaatata ccacctattg ccccccactt taatgcattt ttaaagtaac aattattaac
      841 aagatatctt ttacaagcat tttttttaaa atttctcatt aatttattaa aatctctcct
      901 taagtctcta aagcagtctc cattaaatct atttccaaaa ttagaccaca taattctcca
      961 ccctaagtat tgcttataca ccattatatg aaaaaaaact tgttttctcc ataaaacaag
     1021 ttctttttcc taaaagttta ttataaaatt atgtttatac attatcttta tttatattct
     1081 tataataatt taatgtgttt atatctaact aatctcctac cccaaagtct gtattttcaa
     1141 ataatacttc tatggtttta ttatccttta agaaagaata ctctttttca aaccatttaa
     1201 taagctcctc gtctcttttg aaagaagttg tgttttcagt ccatatatta actgttccat
     1261 acttaaaatt atttaaaagt atgcccatat ctctttttat catatcctta gtctgtccct
     1321 ttattccaac cattaagcat attgacttaa agtatttttt tacttcttct acatcattaa
     1381 atctagcatt cttgtttaaa atatcatttc taaaataatc gtcaaatgtt tcaataccag
     1441 ttttaaatat tataggaact ccaaagtatt cttctatctc ctttaatcta tttctataac
     1501 accaatgtgc ttcaaagaaa agttttttaa tatttttttc aataacaatc ctttttatct
     1561 cttctaaggt ttcctttgga agctcaaaaa cacttccaga gtttattact tctaaaacgc
     1621 cataaattcc ttttacattg ctaagaactt ctttatttaa ttttattatc tcttcagtat
     1681 tttttgagtt atcttctata taatcacaaa aagcacactt tccccataca caaggaaaag
     1741 attttagaag aactatctct ctattatttt tattatctat aacattatat ctatccaaaa
     1801 ttttttcatc tccatttaat tattctgtac tcttaacaaa atatttttag gcaatcttac
     1861 aacaacctgt aacattatta aaactgctac taatttatca caataatctg ttaaaaattg
     1921 tgttataaaa gcacttacca cagggttaac ccctaaatta tttaaaataa ctattatata
     1981 actagaacct gatgaagtaa taccaccaaa tatataggca gttaaacaag ctcctgtaaa
     2041 acttacaaat agagtcatta caaagacgct taaaaataat ttctttcctt tcataagccc
     2101 tttttcataa aaatatcctg caaaaaatcc agtaaatatt tgaactggtg caaaatataa
     2161 agaatatata tcaaaggtta ttccactaac cacacttcct aagactccag ttatcattcc
     2221 ataaacagga cctaatacaa atccaaccat aattgtacct atagaatcta aataaatagg
     2281 taatcttaaa tttaaggcta taaaagctcc aattatattt aatgctattc ctattcccat
     2341 tattgttaac tttaagcttt ttgaatttct catattaata ttccctccct attaaatacc
     2401 atactttgga ttattaagta tattttcaat atcctcttta taacttggga atattttatt
     2461 aagaaagctt accataaatt ttttgctatt aactttatct aaaacaaata cattttttct
     2521 tctcttataa aaatctgcaa catcaacaac gctttgcccc attgatatat cttcgcaagc
     2581 tatgtccaca taagctttaa aaccttcaca aatatctcta tttataaaaa attctactgc
     2641 taagggatca tttataacac atccaagggt tctctcttgt tcccaatgga aatcaacata
     2701 aaatctagta atatcatata taaaattacc aatttcatca ttaaattgat gtatcatttc
     2761 tcttaaattt ggtgttaaaa ctatctttct tgtaacatct aaaccaacca tggtaaattc
     2821 accattaaac ttttttagaa actcccttgc tccatgagga tctacccagt aattaaattc
     2881 agctactggt gaacaatttc catgactttt ataagctccc cccatagaaa ctatttcctt
     2941 aactctatga aaagtttctg aatccttttc tatagctcta tataggtttg ttaggggtcc
     3001 aagagcaata atactaacat tctcctgatt ttttaaagta tttaaaataa aatcaacacc
     3061 attttctcta atatactcac tagatacctc ttctaaaaaa gtttctccta agccatcttc
     3121 cccatgagta tcctgtgctg ttacaagctc tcttttaact ggcttacttt ctcctaagta
     3181 aacaggaata tccaatctcc ctaaaagctt aagtgccttt aaggcatttt tagctccctg
     3241 cacgctttca acatttcctg atacggtggt tattccaaca acctcaatct cttttgactt
     3301 taatgcaaga ataatggcta aagcatcatc tatccctgga tcacaatcaa taattacctt
     3361 tctcttatcc attttaattt cctccctttg atttaacttc accaaaagga actgttatta
     3421 tctttaagct tcctaataac tatcttttta ctaagataaa ttttaaaact aatcttacta
     3481 agctttataa taatcctaaa aaaaaaatta atcacttatt aaaaacataa tttaactctt
     3541 catttatact ttttaataat cttagttaaa atttaagtta gttattaata gctaaaattt
     3601 catatttact tacaaaaaag cttataaatt cagcctaaaa ataataaaaa ctatattaca
     3661 ggcatactaa agagtataac tatcctataa tacagttaca gcccttagtt ttttatagag
     3721 ggatggttac gaacctctac caaattactt tggatttttt tataaaattt taggtatgat
     3781 gaaataccac accataccta ttatataata aaatcacata aattcaaata atgttattta
     3841 tccaatatta tgttttaact gtaatattat ataccaaaaa aattatatga attcaaaggg
     3901 gtctttagcc tcttttgaaa caataaattc cacatctgaa tctaatcttt cttttacttt
     3961 ttcacttact ttaattaaaa caggccactc tgaattaaaa tgtcctatgt ctagaatatt
     4021 taatcccatt tccttataat ctgaaacaaa atgatatgtt gtatctccag ttattattag
     4081 atctgctcca agtttttttg catctccaaa gaaatcttgc ccactaccat ttacaatggc
     4141 aattttctta ataacttcat ttaaatctcc tgcatatctt aaattcttaa tacctaaaga
     4201 tgattttata aggtttatta tttctaggac agtcatttcc ttagttaact ctactactct
     4261 accaattcca gcctgagaat taactgggct tttatccatt atgattcctt tattaaatcc
     4321 taagatttca actaaggtat catttaatcc acctttaact gagtcccagt tagtgtgcgc
     4381 tgaataaaga tttatatcat tttttattaa gcttataatt tttctaccta atagagtgtc
     4441 tgttgttatt gtacttggct ttctaaataa aagtggatga tgagttaaaa taagctctgc
     4501 acccatttcc ttagcctctt taataacctc ttcagtacaa tctaaagcta ataaaatctt
     4561 agttatattt ttttctctat ccccaaccat aaggcctaca ttatcaaagc cttcttttaa
     4621 attaactggg gcaatatctt ctattatatt tataatgtca tttaatttca ttataacatc
     4681 cccttaagtt tatttatctt ttcttctaaa tcactttttc tctttttagc tgcttctgta
     4741 tcttctttta taaaacttaa tatagtctca catctattta ttttttcttc tataaactcc
     4801 ttaaataatg gatgaccttt ttctcttaat aaaggactta cttcatattc taattcatcc
     4861 tcaaatacta gtttttctga attttcattg tattttactt taaataattc ataaaatctg
     4921 ccttcatctt taattaagtc ttcatctatt atttcataat cattcttata taaaaactct
     4981 cttaaaacct ctggattttg agctggctgt aatattataa aatcatactt ttttacctta
     5041 tccatatcag ctaataaaat atctctagtt agatttccac ccattccagc taatataact
     5101 ccatttactt ctcctacttt taaaggatta agccctggtc ctaaaaggca tttaacctta
     5161 tttgaaactc cttcaaaagc aacattaacc tttgcttttt ctataggtcc tttatttata
     5221 tctgaagcta ttgctttttt gcatattcct tctttaacta aatatatagg tatgtatcca
     5281 tgatctgtac ctatatcagc tacactttca catttatcaa catgttctgc tattcttttt
     5341 aatctcttac ttaactccat attatcccac tttctacaaa taatttacca gtgtttgtat
     5401 ttgagaaacc aaaaacacca atgatattat taaagacaaa taaatatatc tttgagctaa
     5461 ctttttatca tatttagcac aaaaatgact tgcataataa atacatacta acagaatcat
     5521 attcattatt ccatatataa tttgtcttcc tacaaaatcc ttcgcacttc caatagtaca
     5581 aggtataaaa tgtgcactaa aatcaattgg cattttatca ggcatagctc ctgataaata
     5641 taatatagaa ggtattagta taatgcctaa tattaataag gatgttatta taaaaggaat
     5701 ataaaatccc ttatccctta ttattgctct tgcattatta acttttacct tgaaactttc
     5761 ttcctttatt ccctttgata ctattaactc tttaaacttt tcaaattcat ctggagataa
     5821 tccataactt atatcctctg tatgtatata tataaccctc ttactagaag ttacaaacat
     5881 acgagcaatt cctacattat gaacaacaca tctaccaaaa caatatttat gttttccaaa
     5941 accagataat ttaaatccat ttataacttt atcttgttct aaaaaaccat ttacatcttt
     6001 aaaatttatc ctaaattttt ttaatccttt aaaacaattt atttcaatgt aatcatcatt
     6061 aacttcgtaa actaaggata aatcaagtaa aatgtaataa atgcatacaa tgctataaac
     6121 aaagcttgat actattacta aatttctctc taaataacca ttcactatgt tacttaatat
     6181 aattaatagt gcatttccta ataaaagata cgcaaagact tcaaatacgc catggcctct
     6241 tttagcctta aattttgtca tttactactt cacctcgttg taattatatc ataaaataaa
     6301 aatttattca gaaactagct tctagcctta ttattacctt tataatttac ataaatcata
     6361 atttttatat atttattttt taaattcaaa atctttttca tataatttta aacttctcat
     6421 attatataac attaaaacct ttttaaatat taactaaaaa aagattagca ccttttaagg
     6481 cactaatctt aagatactaa tctaaataat cttttaactt tttacttcta cttggatgtc
     6541 ttaattttct taaagctttt gcttcaatct gtctaattct ctctctagtt acgttgaatt
     6601 ctttaccaac ttcttcaaga gttctagctc ttccatcatc taatccaaat cttaatctta
     6661 atactttttc ctctctagga gttaaagtat ctaaaacatt tattaattgt tcttttaaca
     6721 ttgtaaatgc tgctgcctct gctggtgctg gagcatcatc atctggtata aagtcaccta
     6781 aatgtgaatc ttcctcttca ccaattggag tttctaatga aactggttct tgagctattt
     6841 ttaagatttc tcttacctta tctactggca tatccatttg ttttgataat tcctctggta
     6901 atggatctct tcctaactct tgaactaatt gtctttgtat tcttataagt ttatttatag
     6961 tttctaccat atgaactggt attcttatag ttcttgcttg gtcagcaata gctctagtta
     7021 ttgcctgtct tatccaccat gtagcatatg ttgagaactt ataccctttt ctgtaatcaa
     7081 atttttcaac agcctttata agacctaaat taccttcttg tataagatct aggaataaca
     7141 ttcctctacc aacatatctt ttagctatac ttacaacaag tcttaagtta gcctctgcta
     7201 attttttctt agcatagttg cttccttctt ctattttttt agcaagctct atttcatcct
     7261 ctgatgataa tagtggaact ttacctattt cttttaagta cattcttaca gggtcatcaa
     7321 tggcaatacc ttctggaaca gaaagatcta attcttcctc ctcttcctct tcttgctcga
     7381 tttcacttat gacctctata cccattgatt ctagaacctc atagattttc tctatttgtt
     7441 ctggatttaa ttctatttca tcaagctcat ccattatctc cttgtaagtc aatgaaccgc
     7501 tttttttacc tttatctata agtcttttta ctaagaccat tttgtcaact ttttctttac
     7561 cttcaccttt ttttgctttt gttgttttag ctttcatttt attacctcct tccgccatta
     7621 aaactactta ctcctttcta gttcttttaa tttatcactt atttctttag ctctttttac
     7681 taaatcaagt gactctttaa acatattgtt ttgttcacat ctttttaatt ctttaagtac
     7741 agtagtctgt tcctgttcta acttatattt tctaataaga ttaatataat ctctaacaag
     7801 aacttctata ttagaagaat gatctagaat aagtaattct tttacttcta accattcctt
     7861 taaaactttt ggatcctcac acttagcttc tacatgtttt cctaagttat taaatcctat
     7921 ttcctcaatt gaatcattga ttatcttttc taaggattta atattaggag atattaaatc
     7981 atcatcttct aagtatttct ttactaatga atggttttcc tccatacaaa gaagttttaa
     8041 aacacttctt tcacatttaa cataaggagg ttctacatat aattttgttc catattcttc
     8101 cttatcattc acattttcat ggtttttatt actatttatt aagtttttat ttaataaatc
     8161 ataaaatgct tgttctttta tggatgtttc ttctgaaatc ctctttatgt agacatcttt
     8221 ttctatagga tttaaatctg atagtatatt aacaactctt tcagcatact ttatcaatcc
     8281 attattatct gaaaaatcta taccaattct agccctatga agcttataat ctataagttt
     8341 ttttgcagat tgaattaact ttataaaaga ctctttacca ttagctctta taaattcatc
     8401 tggatctttg ccttttggta cagttagtac tctaacatct aatcctgcat tttgcaatat
     8461 ctcaagtcca cgtatagtag ccatttgccc agcaatatct gcgtcatagg atattattac
     8521 tctatctaca taccttttta ataacctagc ttgattgtct gtcaatgcag tacctaaaga
     8581 agcaactaca ttagttattc catattgatg aagtgatata caatccatat atccttcaac
     8641 tattattaat gttctttcct gtaatccatg ctttatagca aaatttaaag catatagatt
     8701 agttcctttg tgaaagacta atgtttctgg agagtttaaa tattttggct tagaatcatc
     8761 taaaactcta ccaccaaaac ctattacact tcctttgtag tcaaatacag ggaaaataac
     8821 tctatttctg aatctatcat aaaaagttcc cttttcactc ttaatcccta atccagctgc
     8881 ctcaatggct tcattactaa agccttgttt tctaagatgg tttatggtgt tttgccaccc
     8941 ttccttagcg tatccaagac caaagctttt taatatattt tcttttattc cccttcttaa
     9001 aaaatattcc ttagcttcct tattattttg aagattagaa aagtaatatc tagcagcttg
     9061 cttatttatg ttatataaaa cctgcttttt cttttgagat tcgctttgct taccatcctc
     9121 tataggtatt attatattag ccctatcagc taactcttta acagcatcta caaaatttaa
     9181 atttttagtt ttcataacaa aacttattac atttccagct tccccacatc cgaagcactt
     9241 aaatatttgt ttgtcaggag aaacactaaa agatggactt ttttcattat ggaatggaca
     9301 taaaccagaa aaatttcttc cagctctttt tagtcttacc acatcagaaa caacatcaac
     9361 aatatcgttc tgctctttaa ctttttctat aatttcttct gaaattcgca agaaatctac
     9421 tcaccttctt aatatttttt gacaattaat tattattcga catcaattga taaaatcctt
     9481 ctcaaatttt aaatataact tttttttaga aatttttcaa gtgtaaacgc ataataatta
     9541 tattttagca atatttagcc ctaattattc caaaaatatt taatacatca caaactttgg
     9601 aatatatatt ttattgaact catttgtaca gtaatcatct gtcattcctg caatgtaatc
     9661 cgtaacccct ctatgtattc cttcctcttc acatatattt ctatatagtt caggcatttc
     9721 tccccaatta ttgtaataat actctataac cttctctagt acaaattctg cttttttcct
     9781 ctcatcctct aagtatttcc ctaaatatat attatcaaac aaaaattttc taagctgaac
     9841 aaaggcatct cctatatctt tgcttaaaga taccctaggt tcccctttat ctatattatc
     9901 aattgtatta aaaacacaat ctttaactaa agtgtctatt ctttcactac cactagcacc
     9961 taagatttca ataatattct taggcaaatc ctcttctttc aaaattcccg ctctaattga
    10021 atcgtctata tcatgattta catatgctat tttatctgca aatctaacta cttgtccttc
    10081 aaaagttcct gctaccttac taacatttga aaaaccacta tgatttaata ttccatctaa
    10141 aacttctttg gtcaaattaa gacctaatcc atttttctct attttctttg ctaccctaac
    10201 actttgttca ttatgcttaa acccacctgg aagaaaatca tttaaaacat cctctccaat
    10261 atgagcaaaa gctgcatgcc ctaaatcatg acctaaagca atagcctcta ttaaatattc
    10321 atttaatgat agagctactc ctatcgttct tgctacttga gaaacttcta aagtatgaac
    10381 aagccttgtt ctataatgat ccccataggt tcttataaaa acctgagttt ttctttttaa
    10441 tcttctaaaa gatttactat gaattattct atccctatct accataaaac aggttcttat
    10501 ctcatctggt tcttctaagc gttctcttcc tacagattca tctgagaact ttgcgacttt
    10561 atttaacttt attctttcaa aattttcaat attctctctt attttcatag catcacctac
    10621 tatatatatt ctatgtacct atttaaaatc ctttataatt ctagagattt aaaactttat
    10681 attttttata aaacaagctc tcaatgaatt ttttgacttt aaatcaaact atttcatatt
    10741 ttaaagtata atctaaagtt aatcataata ttcttataat aaggaatttt ttattaggag
    10801 gttaatcatg ggaaagcatt ctaaaaagtt taatgaagct gatgaaattt ttaatgctgt
    10861 agagtccata gttttaccta tgtataattt agaaaactat tctatagaga atataaaatt
    10921 taaaaataca gataaaaata gagctgttta taaattaatt gatgatatta ataatcctaa
    10981 aaatactttt tgcttaaaaa aggtttatta tgatgaaggg actctcttat tcatatattc
    11041 agttatggaa tggtttgcta gaaatgaaat taagcttcca aaaatgctcc cttcaaagtt
    11101 taatggtaga tttgttaaag caaataatat gctttttatg ctctgtccat gggttaaagg
    11161 tgaaaaatgt aacttcgata acttacaaca tatcttacta tccatagaaa atctagctaa
    11221 aatgcataat tgttcaagaa actttaaagc aattgaaggt agtttaatta aaacgggatt
    11281 tgatagtctc tacatatcca cattaaaaca ctttaataag attctttcat catttaatac
    11341 tgcaactaaa atgaaacata aggacaagtt ttcatcaata tttttagatg tttttgatga
    11401 aaatatttat ctagctaaag aagctctctt agtttcagga gctattaata ataaaaattt
    11461 aagtagatct ctttgccatg gagattatgt aaataaaaat attttaattg ataatactga
    11521 tgtttgggta attgattttg ataaagcatc cttaaattat tctatgtatg atttatgtta
    11581 tttcatgaga cgtttattaa aaagatcaaa tactaattgg gatatagact taacaagaaa
    11641 gataattaaa acatataatt caattgctcc tcttacagag gatgacttca aatatgtttt
    11701 ttcatatcta gcatttccac aaaaatattg gcgcttatca aaggactatt ataataacat
    11761 aaaaaaatgt aataaatcaa tgtttgtaga atctcttaag gaagttgcac tagatactta
    11821 tgctcaggtt agatttgttg aagaacttag aacattatta aaaaaagatt ttctacttac
    11881 tctataagct tatttttatt caaaaatggc gtgtatcgaa ataaatttta ctaaaaaatt
    11941 attttgatat acgccttctt ttattaacta ttttttatat cattaactat caaaccatcc
    12001 tcaatattta ttattctatg gcattgagat gctatgttta tatcgtgagt tactaaaatt
    12061 atagtagttc ctcctttatt tatatcctta aaaatattca taacctcgtt acttgttctt
    12121 ttatcaagag cccctgttgg ttcatctgct agaataattt taggctcatt tatcaaagct
    12181 ctagctattg caattctttg acattgtcct cctgaaagtt cagtaggata tttattaaat
    12241 ttatctaata aatctaactc ttttaatatt cccattacct tttctttctt attcttaatc
    12301 tttttatcac tatattctaa tgggattttt atattttcat atactgtata atcatttaat
    12361 aactcaaatg actgaactac aaatcctaaa ttagcatttc taattttagc aagttccttc
    12421 tcacttgtat cagtaatttc tctaccttct agctcataag aaccagagtc agcggaatct
    12481 aaacacccta atatatttaa taatgttgtt tttcccgaac ctgaaggtcc cattatagct
    12541 acaaactctc cagattttat agataaattt atatttctta atgcatgtac aatattttcg
    12601 ccctttccat acgttcttac tacagatctt agttttatta aatccatttc ttattcccct
    12661 tttattaaat aatttaactt atattttttt atataatata taggaatcat tgatactaat
    12721 attatagctc caataattat tgatatactt ttagcaaaag ttccatattg aaattgaact
    12781 acttctgtaa caaaattaac actatgtaac atcaaataag agtatgctat tattgtagat
    12841 ataaaaacta atataaattg ttcgattata actctgaaac aaatatcact tattgttgca
    12901 ccatgcatta tatgaatgga aaattccttt cttctatcta aaaccctatt acaaaatata
    12961 acaacaaatc ctccaatagc aaaaattcca acaatataga taagtaaaag atttaagtta
    13021 atattatatt ttatctcttt agtaaaagta tctatatttt catttggatc ataaactgat
    13081 attttaactt tattgttctt agctaaactt tctacctctt gctttacttc attaaattct
    13141 ctaggatcac tattgtaaac taaaaaggca ttacttacat ttgcattaat aatatcctta
    13201 ggcattggag taataataaa tgtgttaaga tttttaaccc tacttaaatc ataaactccc
    13261 ttatctaaaa aaaacctatt ttcttctata aagcctacaa ccttatagtt aaaatattta
    13321 ttttctataa tatcacctat attaaaaatt cctttataag catttcctaa tactattgga
    13381 atatatttat tctcccattc aacagaattt aattgagaaa atttttcaaa atcttttaag
    13441 cttccctctg ataaacctat attaaaagta tcaaaaaaat ttggactaca ttgaagcgaa
    13501 taaacagact tcatttcttc cccttgctct gactctttaa aatcattatc taaaaatttc
    13561 tctattccat taaaatatgg aagttttatt tcccctaaaa cagttgtaac catgttaaaa
    13621 ttcttatcaa tatctaaagc aaagttagaa aatttatttt catccttttg gtcttctaaa
    13681 aactcatcaa tagagtcata ctttaatgct atttgataaa tagttttatt acctattatt
    13741 tcattcatag aactaagttt ctcattttga taatttttag attcaataat tctataagtc
    13801 aatattataa ctaacattag ctgtaatata agtacaatct taaaaaacaa actatttttc
    13861 aactctctaa tagagtactt taaattattc atttcagttt tcccttcatt atattaatag
    13921 gtttcacctt ttttgcctta aataaaggta ttaaaaccgc tattaagcca ataaacataa
    13981 atattatagt tataaaaatt atactttgat agtaaacatc aaaagtaaat tcaggaaaca
    14041 tataaatcaa aagtcttttc aatagtaaat gtaaagaaac tcccattatt aaggcaatac
    14101 atatgcttat taaatactgt tttaatatat gtattactat tttaccattg cttgcaccat
    14161 aagcttttct taccccaatc tcctttatac tctttttaat ccaataatat atagctatta
    14221 ttagatttaa aattcctatt ataaaaacta aaaataaaat atctataaag tccttatatt
    14281 catttaaggc ttcctttaaa ttaaaatctt tctttatatc ctctaatgaa aaacttgtat
    14341 tcttatttac tgctaatttt ttataatcat ttaaaatatt ttttaacgct tcattattct
    14401 ctatgtttat tttccatgat gaacgagtgt ctgaaaattc atcgctagat tttaagttaa
    14461 ataaaaatgt attatcatat ccagtttgcc tctcactact tcctaaaata ccaatcacct
    14521 taaatttctc ttctcctata gtgaaaaatc tctcattgtt ttcttcaaaa acttcatctt
    14581 taaaatttac tccaactaat gccacctttt ctccattttc tatttcttct ttagtaaaat
    14641 ctcttccttc taatatggga aaactttttt gaaaaccatt tttaaaatat attcctcttc
    14701 caaaaatagt atcattatta agatacttag gtaaaggttc atactctaaa taaatttgtg
    14761 aattattata attatcaact atctcacaga cttctctaaa agtaacatta ttagagtcat
    14821 taaacttaaa atatttttca gttaagtttg aaatattatt tacttcttcc ttgtttttat
    14881 aacttaaaac taaaatactc aaaacaattg aaaaagtaaa tactattaaa gtaaatgtcg
    14941 ttattatata tgttaatttt ttatattttt taaaactaat catttttcac ctattaatag
    15001 cttgtactat taaatagtac aagctatttt cataatatta tttagtataa gcgtgcaaaa
    15061 gttcttgaac ttccagttga tgtaccaaca cttccatatg catttctacc accagtacga
    15121 gttatagtgg atgaatgtgc tcttgtattt ccagtatttg atgccccatt accaattcta
    15181 tatccatcag cattaacaat ttctacatat gcatatgctt tttcatttcc ggaatctata
    15241 actgtagttg ttgctccata acaattagat gaatttctac ttccctctac ccatccatgg
    15301 tatgatgttg ttttagatgc tcctgaaaca gttgtaccct gagctgcact tactaaaata
    15361 ccccctgaaa gcataaccat tgaagcccct aaaataatac tttttttaat cataaatacc
    15421 aattcctttc tt
//
LOCUS       DAEVGI010000050        15358 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEVGI010000050 DAEVGI010000000
VERSION     DAEVGI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 15358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15358
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.050"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            186..623
                     /gene="fliB"
                     /locus_tag="K8M77_001304"
                     /pseudo
     CDS             186..623
                     /gene="fliB"
                     /locus_tag="K8M77_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
     gene            813..1280
                     /locus_tag="K8M77_001305"
     CDS             813..1280
                     /locus_tag="K8M77_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085808.1"
                     /translation="MKSYERKLKIEIITILVMIDYILMIRRANSDIEELVLAGIALLW
                     TTFALGYHIWDLKKRGLTFTDGIKSFIDRIKRLKYFSRIYTKMFKYGTGIIIILGMVL
                     VALNVINTEVLNSSFIKYIINIGMSLAFSLGMIEAIVFCMAGIVRIMEENKEI"
     gene            1471..3345
                     /gene="hisJ"
                     /locus_tag="K8M77_001306"
     CDS             1471..3345
                     /gene="hisJ"
                     /locus_tag="K8M77_001306"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBI7085809.1"
                     /translation="MKNYYLEEVVDEIYYKEGKENRELKMDILSLKIKNELNIIENLC
                     GNCKRKSNLYEAIKNVKSLLKQYYIVFNGTQDALFLVEMLKDGNFKYVRNNNAYLQDF
                     GLKGEEIINKTPKDVFGEELGQRFCNYYKKCIENKKVIVFEDDLSLNGKKRVFLTKLL
                     PIIDEDDGVFIVGSREDITKRKEMEIELDRMANYDELTNIPNMRLFFKSFGNTINESK
                     KKGKKFAVLFIDLDWFKEINDNFGHDVGDEVLVCAVKRIYKCLRKGDILGRIGGDEFA
                     AILKDISDKEEIEKIVKDIQNYLRKRIKIGDVTCNIDSSIGITIFPEDGEKIEVLMRN
                     SDKAMYKVKNREKGGYRFFNNMIREYNFKNKDGHVHTKYCPHGSDDNIEDYIEEAIKY
                     KLDEISFLEHLPLPESFIDPSPLKDSAIKLEEMESYLKEGNKLREGYKSKIKVNVGVE
                     VDYIEGYEIETELLLNKYGKHLNDSILSVHMIKGNKRYYCIDFSESEFKKIIDDLGSL
                     EKVYDKYYDTLIMALKSDLGPYKPKRIGHLNLVRRFNKEFPYNYEKHISKIEKILDII
                     KEKGYELDFNIAGLRKKECNEFYIEGKVLEMAIEKDIPMVLGSDSHSAKYIKCIKEFL
                     "
     gene            3626..5458
                     /locus_tag="K8M77_001307"
     CDS             3626..5458
                     /locus_tag="K8M77_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBI7085810.1"
                     /translation="MSKITREAIHHIAQSNYSYGYDNETLHLRVRTKKGEVNKVEIRI
                     GDPYIWDEGGCDGGNMNATGGRWTGGKSYPMRKECETKYFDHWIVYYKPLTKRSRYGF
                     ILYGDEETLLCTEKRIEELDGKYDEEKLSAIGNFYCLPYLNAIDVAKTPQWVKDTVWY
                     QIFPDRFCNGDKSIDPENVEPWGTEPTRDNFMGGDLQGVLDKLDYLCNLGINGLYFCP
                     VFEATENHRYETIDYFKVDPALGGNEVFKKLVSEAHKRGMKIMLDAVFNHIGYFSPKW
                     QDVLKNNEKSRYKDWFCIKKFPVLENGLENVDGNNLNYETFGRIATMPKLNTENPEVV
                     EYLLKVAKFWVEEMDIDGWRLDVCNEVDHVFWRKFREVVKETNKEVYILGEVWHDGLP
                     WLMGDQFDAVMNYPVTDAVKEYFCLNQSNPEDFKYMIEANKVSYLRQIGETIFNLLDS
                     HDTPRILTVAGGNKDKMKLAYLFMFTQAGSPCIYYGDEIGMEGNQGMGMEFHRRCMIW
                     DENKQDKDMLKFMKQIIKIRKENKELNLLDNNWIRANRDENILIYSKKNIFIIMNNSD
                     KEEKVFLPKEIKNNKVKDLFEEKIEPLKEDVELKPFAFKVYKKL"
     gene            5698..6981
                     /locus_tag="K8M77_001308"
     CDS             5698..6981
                     /locus_tag="K8M77_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBI7085811.1"
                     /translation="MRELYLKTLKKEVVPSEGCTEPIAIAYAASIAAEYLKGEIKEVN
                     IYLSKNVIKNALGVGIPGTGGVGIEIAAALGISIQKSYKKLTILSNFTEDELKKAKEI
                     VDKNIINIKQKNTNKALYIEVELLSETSKSKVIIEDTHTNVTLIECDDEIIMDNNSEV
                     SEDLEEDYNLFKIADIYNFAKEADFDDIKFILESAKMNEKVSEEGLKGDYGLQVGSKI
                     IQKGNFNLFSNDASNKIIAASAAASDARMDGCAMPIMTTAGSGNQGIACSIPVAQTSR
                     LLDKSEEELARALVLSNLVTIRIKKHMGRLSPLCGAGIAGATGASCGITYLLGGDLKN
                     INYCINNMISDLSGMICDGAKETCALKIATGTNAAIQCANLAINGISATANDGIVAKD
                     VEETIESIETLIQNGFKNVDDTILNIMLEKKKNNK"
     gene            complement(7268..7771)
                     /locus_tag="K8M77_001309"
     CDS             complement(7268..7771)
                     /locus_tag="K8M77_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085812.1"
                     /translation="MNKTNKFKSTVSTLIMIALSFLIIYLLHDISKAVVGLLCGGKIE
                     FNRGITIVNADYGNFSYILNLILGISIPVLFLFLLVLIFNNSLRNGLYHKFTLIFFMC
                     SVLSISVCLIFYPLVSLFTDLRLHTELDELLEATGLSPLFILVLSLLITTLLVKIASK
                     KNLFKNL"
     gene            8138..8683
                     /locus_tag="K8M77_001310"
     CDS             8138..8683
                     /locus_tag="K8M77_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085813.1"
                     /translation="MKKLIAILSVIICLGLGGWLIASHYTNLKEERFNNIIENANKAI
                     ENKEYETAEVLLEKAKLMNNKTQVICEKIQNLEFHKEQQELYDQGLKLEKLKRYSEAI
                     DVFSEINIDSNDLKRNSEKEIEICKKCIIDDFSNKADKAIRRGDIEKAKYIIAKIERV
                     DSDSPEINILNMKIESFKSAK"
     gene            complement(8721..9341)
                     /locus_tag="K8M77_001311"
     CDS             complement(8721..9341)
                     /locus_tag="K8M77_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI7085814.1"
                     /translation="MLKKKILARISILIGLPILVFLIFEVFLTYKMMNFKDINQLKDV
                     EYEIILGAGLYGDKPSPILQERLEEGLKYLQLHPNTKVIVSGGQGSNEPIPESEAMKI
                     FFVNKGINPNRIIEEDKSKSTFQNLEFSKKILEERHANKDEVLIVTSDFHLFRAKEIA
                     DYLNIKNEGLPSKTPIFLRVQYMVREFPAMIKLLLQEAFLDTKDIV"
     gene            9604..10548
                     /locus_tag="K8M77_001312"
     CDS             9604..10548
                     /locus_tag="K8M77_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI7085815.1"
                     /translation="MKGLKKLAILGIATLTVGMFVGCSSSKTSEESNTSNEKRIVQSV
                     KGEVEIPANPKKIVDISGSSEELVLLGHTPVATANVDSYKTDQVPEYMAETLKDTKIV
                     GHSMMDTADIEGILSVEPDLIIMSPRQEKIYDQLAEIAPTVIINDNANDWEAKFKEVA
                     KLFDQEEEAQNWLDNYYKKAKEVGEEIKKVNGDGTYLSVLASSGQFMVFTEGGIGTVL
                     KSDMGLPQPSKMPEQDSITLPTVTLEGLTEIDADHIIVLGTESDKADLESNSVWNEMR
                     AVKNGNVKILDVSPYFSQAYNPMGRELLLESIKNELVK"
     gene            10647..11660
                     /locus_tag="K8M77_001313"
     CDS             10647..11660
                     /locus_tag="K8M77_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI7085816.1"
                     /translation="MKIGRKKTLTYGLMFIGLLLLLSGIILSISIGAKEIDFSMIINA
                     IFTNDADINTNIIRDVRIPRALAAALVGGFLAVAGAIMQGITRNPIAEPSVMGITQGA
                     TFMIAVAFVLQRIYPNLVINSFALMIFAFLGASISGLLVYFISSRASRKVDPVKLALA
                     GTALGTLLISLAMGISMYFNLSQQLSFWVSGGLVSAKWEGVILLALIGGIAFIGAMIM
                     SPRITILSLGEEVAIGLGEKTNLVRFISIMIVICLTGASVSVAGNIVFVGLIIPQICK
                     GIVGSDYRYIIPSSMILGAVLLVYSDILARIINPPYETPIGSLTALLGVPVFIYLVRK
                     GGK"
     gene            11664..12677
                     /locus_tag="K8M77_001314"
     CDS             11664..12677
                     /locus_tag="K8M77_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI7085817.1"
                     /translation="MNIKKNHKFIWVTGILIGLIFITFLISLNMGSLSIEPGEVIKTL
                     IGQGTKSNEIAIFKLRLPRIVIGILVGTALATAGTILQGVTKNDLADSGILGINSGAA
                     LFIVVYIYIMNGNVYDGVSNLTIFTMPIVALSGAIFGAFLIYILAWKNGINSSRLLLI
                     GIGINIAFTSLLTIFQLRFTTQEFNRVMAWISGSIWGASWKYVLAILPFIIIFMALTI
                     YKSRYLDGLNLGDEVATGIGIEVEKERRKLIIYAVILAGVATSVAGSIGFLGLVSPHI
                     ARKLIGPKHKKLIPVAALIGSLILLVSDTIARNLIAPMEMPVGIIVSIIGVPYFIYLM
                     LSK"
     gene            12716..13498
                     /locus_tag="K8M77_001315"
     CDS             12716..13498
                     /locus_tag="K8M77_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085818.1"
                     /translation="MNCIATDNLNISYGSLDIVKNLNLNIPKGKITTIIGANGCGKST
                     VLKTIARILTPKSGEIYINEKNIKEQGAKELAKVMAVLPQSPQAPNGLTVEELIAYGR
                     FPHQKGFGKLKKEDKNIIQWALEVTGLIEFRERDIEDLSGGQRQRAWIAMALAQQTDI
                     LILDEPTTYLDLAHQLEILNLLKELNENHGTTIVMVIHELNNAARFADYMIGMKAGKI
                     ACQGTAYEVMTKENLKELFNIDAEIIEDPRNKRPVCVTYDML"
     gene            13810..14297
                     /locus_tag="K8M77_001316"
                     /pseudo
     CDS             13810..14297
                     /locus_tag="K8M77_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460320.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            14375..14590
                     /locus_tag="K8M77_001317"
     CDS             14375..14590
                     /locus_tag="K8M77_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HBI7085819.1"
                     /translation="MYEYKFINVPVDKSFKCKRGDSFEKCKDIIKEEVKNGWRLKQIV
                     TPINEKSGVNSTYAYEIIFERKVENYA"
     gene            14583..14899
                     /locus_tag="K8M77_001318"
                     /pseudo
     CDS             14583..14899
                     /locus_tag="K8M77_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15111..>15358)
                     /locus_tag="K8M77_001319"
     CDS             complement(15111..>15358)
                     /locus_tag="K8M77_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085820.1"
                     /translation="LAYYLLYFKRIENYYDMNQIKNKDHIADLIGITFIIIGTILVLT
                     ECVFFVFKFEDAYLLIPLVGCFIYYVSEMFEINKKIS"
BASE COUNT         6175 a         1415 c         2715 g         5053 t
ORIGIN      
        1 aaaccaagca aatatagtag taatacaaga catatcacaa acaagataac ttgtatatgt
       61 aggatgcgtt gtatccgaat ttacgccatc gaagagttgt atcaaaggaa attagattac
      121 tattatagtt ttcaaaatcg gactcgtaga acagggaatt aaacaaaatt tatatatatt
      181 tatagatttt tggcaacgga gaatgtgaag atagctgttg tataggatgg gatgttgata
      241 ttgataagtt tactttttat caatatgaaa atgtaagtaa tagtgatatg aagaatatac
      301 ttgaaagcaa tcttattaaa aataagaagt gtcaatgtga tgaaattgat tttgctaaag
      361 tgaagttagg ggaaagtaaa agatgtccat ttttaaagtg tgataactac tgtgttatcc
      421 attctaatct tggagaagag tatctttcaa atgtttgtac atcatttcct agggtaacta
      481 ataagattga tggaatttat gaaatgtctc ttgctgtggc atgtccagag gctgctagaa
      541 ttcttttact taagaagaat ggtattgagt tttcagaaag tgacgaaaac ttaggaaaac
      601 acataatatc aagtgaggtt aattttattt aattgactta ctaaattata taaaagagca
      661 tgattttaat aatttagagt ttgtgaaaac tctactttct taaagattat atattttaaa
      721 atttagagta ttatactttt tttaaaattt atttacaaag aaactttaaa ttgataatat
      781 ttatatagga tttaaaagat ttggagggga gcatgaagag ttacgagaga aaactaaaga
      841 ttgagattat tactattttg gtaatgattg attatatact tatgataaga agagcaaata
      901 gtgatataga agaattagta cttgcaggaa ttgctctttt atggacaact tttgctttag
      961 gataccatat atgggattta aaaaagcgtg gattaacttt tacagatgga attaaaagtt
     1021 ttattgatag aattaaaaga cttaagtatt ttagtagaat ctatacaaaa atgtttaaat
     1081 atggtacagg tataataatt atattaggta tggtattagt tgctttaaat gtaattaaca
     1141 cagaagtttt aaatagttca tttataaaat atatcataaa cataggaatg tctttggctt
     1201 tttcattagg gatgatagaa gctatagtat tttgtatggc tggtatagta agaattatgg
     1261 aagagaataa agaaatataa aaaatgcttc acaactttgt gaagcatttt ttatttatag
     1321 ataattttag tgtttttata attaataaga tagaaattat ttcattaata ttatatactg
     1381 tatatggata aaatgttaaa aatttatcta tatattgaat tttatttaat aatactatta
     1441 aataacagat ttcacaagtg agggtaagat atgaaaaact attatttaga agaggttgtg
     1501 gatgaaattt actataaaga aggtaaagaa aatagagaac taaaaatgga tattttaagt
     1561 ttaaagataa aaaatgaatt aaatatcata gaaaatttat gtggtaattg taaaagaaaa
     1621 agtaatttat atgaagctat aaagaatgta aaaagcttat taaaacagta ctatatagtt
     1681 tttaatggaa ctcaagatgc attattttta gttgaaatgc ttaaagatgg aaattttaaa
     1741 tatgtaagaa ataacaatgc atatcttcag gattttggac ttaaaggaga agaaattata
     1801 aataaaacac caaaggatgt tttcggggaa gaacttggac aaagattctg caattattat
     1861 aaaaaatgca tagagaataa aaaagtaatt gtatttgaag atgatttgtc tttaaatggt
     1921 aagaaaagag tgttcttaac gaaactactt cctattattg acgaggatga tggtgttttt
     1981 atagtaggtt caagagaaga tattacaaag agaaaagaaa tggaaataga gttagacaga
     2041 atggctaatt atgatgaatt aactaatatt ccaaatatga gattattttt taaatctttt
     2101 ggaaatacaa taaatgaaag taaaaagaaa ggaaagaagt ttgcagttct ttttatagat
     2161 ttagactggt ttaaggaaat aaatgataac tttggacatg atgtagggga tgaggttcta
     2221 gtttgtgcag ttaaaagaat atataaatgt ttaaggaagg gcgatatttt aggaagaata
     2281 ggcggagatg aatttgctgc tatactaaag gatataagtg ataaagaaga aattgaaaaa
     2341 atagttaaag atattcaaaa ctacctaaga aaaagaataa aaatagggga tgttacatgt
     2401 aatattgatt catccatagg tataacaata tttccagaag acggagaaaa aatagaagta
     2461 cttatgagaa attctgataa ggctatgtat aaagtaaaaa atagagaaaa gggaggatat
     2521 agatttttta ataatatgat tagagaatat aactttaaaa ataaagatgg tcatgtacat
     2581 acaaaatact gtcctcatgg tagtgatgat aatattgaag attatattga agaagctata
     2641 aaatataaat tagatgaaat aagtttttta gagcatcttc ctcttcctga aagttttatt
     2701 gatccttcac ctttaaaaga tagtgctatt aaattagaag aaatggaatc atatttaaaa
     2761 gaaggaaata aattgagaga gggatataaa agtaagataa aagtaaatgt tggagtagag
     2821 gtagactata tagaaggtta cgagattgaa acagaattgc ttttaaataa atatggaaaa
     2881 cacttaaatg acagtatttt atccgttcat atgataaaag gaaataaaag atattattgt
     2941 atagatttta gcgaaagtga atttaaaaag atcatagatg atttaggttc tttagaaaag
     3001 gtatatgata aatattatga tacattaatt atggccttaa aaagtgattt aggaccatac
     3061 aaaccaaaaa gaataggtca tttaaatcta gtaagaagat ttaataagga gtttccatat
     3121 aattatgaaa aacatatttc aaagatagag aaaatattag atattataaa agaaaaaggc
     3181 tatgagcttg attttaacat tgcaggctta agaaaaaaag aatgtaatga attttacata
     3241 gaaggaaaag tcttagaaat ggcaattgag aaagatatac ctatggtttt aggaagtgat
     3301 tctcattctg ctaaatatat aaaatgtata aaagagtttt tataaataag atatgattta
     3361 aagtagtgtt tggtttagat agtaattata tatctaaact aaacatttac ctaaaaatat
     3421 tatagtagtt tttaatttta tttaagtata aaattaaaaa ctttctaggg agtatataat
     3481 aaaaaaaagt tgaacgtttt cataaagagg aatataatta catgcagaga agatatacat
     3541 attaatattt ttttgattta ttttggtatc gttaccaaaa ttgtttgtaa taatttataa
     3601 ttataatatt atggagggtt aatttatgag taaaattaca agagaggcaa tacatcatat
     3661 agctcaaagc aattattctt atggatatga taatgaaact ttgcatttaa gggttaggac
     3721 taaaaaaggt gaagtaaata aagtggaaat aagaattgga gatccttaca tatgggacga
     3781 aggtggttgc gatggaggaa atatgaatgc cactggagga cgatggacag gtggaaaaag
     3841 ttatcctatg agaaaggaat gtgaaacaaa atactttgat cactggatag tttattataa
     3901 accattaacc aaacgttcaa gatatggatt tatattatat ggagatgaag aaactctttt
     3961 atgcacagaa aaaagaatag aggagttaga tggaaagtat gatgaagaaa aattaagtgc
     4021 tataggaaac ttttattgtt tgccatattt aaatgccata gatgttgcta aaacaccaca
     4081 atgggtaaaa gatactgttt ggtatcaaat attcccagat agattttgca atggagataa
     4141 atcaatagat ccagaaaatg ttgagccatg ggggacagag cctactaggg ataattttat
     4201 gggaggagat ttacagggag ttttagataa attagattac ttatgcaatc ttggaattaa
     4261 tggactatat ttttgtcctg tatttgaagc tactgagaat cataggtatg aaaccataga
     4321 ttattttaaa gtagatccag cccttggtgg aaatgaagtc tttaaaaaac ttgtaagtga
     4381 agctcacaaa agaggaatga aaataatgtt agatgcagta tttaatcata taggttattt
     4441 ttcaccaaaa tggcaagatg tattaaagaa taatgaaaaa tcaagatata aggattggtt
     4501 ttgtataaag aagtttccag tacttgaaaa tggcttagaa aatgttgacg gaaataattt
     4561 aaattatgaa acctttggaa gaatagccac aatgcctaaa ctaaacacag aaaatccaga
     4621 ggttgtagaa tatttattaa aagttgctaa gttctgggtt gaagaaatgg atatagatgg
     4681 ttggagactt gatgtatgta atgaagtaga ccatgtattt tggagaaaat ttagggaagt
     4741 agtaaaggaa actaataaag aagtttatat attaggagaa gtttggcatg atggacttcc
     4801 atggcttatg ggagatcagt ttgatgcagt tatgaattac cctgttacag atgcagtaaa
     4861 agaatatttc tgcttaaatc aatctaatcc agaagatttt aaatatatga tagaagctaa
     4921 taaggttagc tatttaagac aaataggaga aacaatattt aacttgttag atagtcatga
     4981 tactccaaga atattaactg tagctggagg aaataaggat aagatgaaac tagcttatct
     5041 gtttatgttt actcaagctg gttctccgtg tatatattat ggagacgaaa ttggtatgga
     5101 aggaaatcaa ggaatgggta tggaattcca tagaagatgt atgatttggg atgaaaataa
     5161 acaagacaaa gatatgctta agtttatgaa acaaataata aaaataagaa aagaaaataa
     5221 ggaattaaat ttattagata acaattggat aagagctaat agagatgaaa atatacttat
     5281 atattcaaag aaaaatatat tcatcattat gaacaattca gataaggaag aaaaggtatt
     5341 tttacctaaa gagattaaaa ataataaggt taaggattta tttgaagaaa aaattgaacc
     5401 tttaaaagaa gatgtagagt taaagccctt tgcatttaaa gtttataaaa agctttaata
     5461 tttaataaat taaaaaaaga gtaagaattt tagaatgtat tatattttat aatttttact
     5521 cttttattta tatttataaa ggataaaatc actatatttt taaggcgcat tgtaagcaat
     5581 aattaaaagt gtaatcaaaa tattataata aatttaaatt tttcttgtaa atgattacaa
     5641 aaattaaggt gtggtgttat aataaaaata taaaggaatt tataagaagg tgagaagatg
     5701 agagaattat atttaaaaac attaaaaaag gaagtagtac catcagaagg ttgtacagaa
     5761 ccaatagcaa tagcttatgc agcatctata gcagcagaat atttaaaggg agaaataaaa
     5821 gaagtaaata tctatttaag taaaaatgta ataaaaaatg ccttaggagt tggaatacct
     5881 gggactggtg gagttggaat agaaatagca gcagctttag gaatctcaat tcaaaaatcc
     5941 tataaaaagt taactatatt atctaatttc acagaagatg aacttaaaaa agctaaggaa
     6001 attgtagata aaaatataat aaatataaag caaaaaaata ccaataaagc tctttatata
     6061 gaggtagaac ttttatctga aacttcaaaa tctaaagtta ttatagagga tactcatact
     6121 aatgtaacct taattgaatg tgatgatgaa ataataatgg acaataatag tgaggtaagt
     6181 gaagatttag aggaagacta taacttattt aagatagcgg atatatataa ctttgctaaa
     6241 gaggcagatt ttgatgacat aaaatttatt ttagaaagtg caaaaatgaa tgaaaaggtg
     6301 tcagaagaag gattaaaagg agattatggc cttcaagttg gaagtaaaat aattcaaaag
     6361 ggtaatttta atttattttc taatgatgca tctaataaga taattgcagc ctcagcagca
     6421 gcctcagatg caagaatgga tgggtgtgca atgcctataa tgaccactgc aggaagtgga
     6481 aatcaaggaa tagcttgttc aataccagta gctcaaactt ctagactttt agataagtct
     6541 gaagaggaat tagcaagagc tttagtttta agtaatttag ttacaataag aataaaaaaa
     6601 cacatgggaa gactttctcc tctatgcggt gcaggaatag caggggcaac aggggcttct
     6661 tgtggaataa cttatcttct tggaggcgat ttaaaaaata taaattattg tataaataat
     6721 atgatttcag atctttcagg catgatatgt gatggagcga aggaaacctg tgctttaaag
     6781 attgctacag gaactaatgc tgcaatacaa tgtgcaaact tggctataaa tggaatatca
     6841 gcaacagcca atgatggaat agtagcaaaa gatgtagaag aaacaattga aagtatagaa
     6901 acattaatac agaatggctt taaaaatgtt gatgatacta tacttaacat aatgcttgaa
     6961 aagaaaaaga ataataagta ataaagataa aaggttaata ttaataatta aaaagtgctt
     7021 aaggcctagc taaagttaat atattactta gaatctgtaa aagaaagaat aaataaaaat
     7081 ataaatgaaa aaagattgta ttaatttacc ttataaaact tttaaatata tttttataaa
     7141 ttataagttt tattattcaa agtttttaat gcaatctttt tatatacttt aacaaaataa
     7201 taactatata tttttttaga taagtttaga tacttaaaat aagttttaaa aatttttatc
     7261 tagaacctta taaattttta aataggtttt tcttagaagc tatttttact aataaagttg
     7321 ttattaataa ggagagtact aatatgaata agggacttag tccagttgct tctagtaact
     7381 catctagttc agtatgtaat ctaagatcag taaataatga taccaatgga tagaaaatta
     7441 agcatactga aatgcttaat actgaacaca taaaaaatat aagagtaaat ttatgatata
     7501 agccatttct tagtgaatta ttaaatataa gtactagtaa aaataaaaat aatactggta
     7561 ttgatattcc tagtataaga ttaaggatat aagaaaaatt gccataatca gcatttacaa
     7621 ttgtaattcc acgattaaat tctatttttc ctccacataa taatccaact acagcttttg
     7681 aaatatcatg taacaagtag attattaaaa atgataaagc aatcataatt aatgttgaaa
     7741 ccgtgctttt aaatttgttt gttttattca atttttttca actccttaat ataattaaat
     7801 attgaatatg aaatagcttt tattattaaa ttaaaaatta ttgagtagtc agttagttta
     7861 gaaataataa tgaaatatta tcacattaaa tgaacaaaat gttaattaat actatattaa
     7921 tataaacttt ttgtgaacat aagtaaaggg ttaaatataa tttttgatat ttaatttcat
     7981 ttaagaggga aataaaaggg gatttagtgg atttaaagta tgaatagata caaatagata
     8041 aatgaatatt tctctgtgta caaacgtttt caataatgct ataataatag tgaacaaaat
     8101 gtaaatgagt tttgtatctt taaaggtgga gatttgtatg aagaaattga ttgcaatttt
     8161 atcagttatt atatgtttag gattaggagg atggttaata gccagtcatt atacaaattt
     8221 aaaagaagaa agatttaata atattataga aaatgctaat aaagctattg agaataaaga
     8281 atatgagacg gctgaagtgc tattagaaaa ggctaaactt atgaataata agacacaggt
     8341 aatatgcgaa aaaatacaga atttagagtt tcataaagaa caacaggaac tttatgatca
     8401 gggattaaaa ttagaaaagt taaaaaggta ctcagaagcg atagatgttt ttagtgaaat
     8461 taatattgat tcaaatgatt taaaaagaaa tagtgagaaa gaaatagaaa tttgtaagaa
     8521 atgtataata gatgattttt caaataaagc agataaggct ataagaagag gtgatataga
     8581 aaaagcaaaa tatattatag ctaaaattga aagagtagat agtgatagtc ctgaaataaa
     8641 tatattaaat atgaaaatag aaagttttaa atcagccaaa taaatatcaa gagtacttga
     8701 tatttatttg gctgattttt ttaaacaata tcttttgtat ctagaaaagc ttcttgcaga
     8761 agtaatttaa tcatagcagg aaactctcta accatatatt gaactcttaa aaatatagga
     8821 gtttttgaag gaagcccttc atttttaata ttaaggtaat cagcgatttc cttagctcta
     8881 aataaatgaa aatcacttgt tactattaaa acttcatctt tattagcatg tctttcttca
     8941 agaatttttt tactgaattc taaattttga aaggttgatt tagatttatc ttcttctata
     9001 attctattag gattaattcc tttattaaca aaaaaaattt tcatagcctc agattccggt
     9061 attggctcgt tacttccttg tccacctgaa actattactt tagtattagg gtgtaattgt
     9121 aagtatttca agccttcctc taacctttct tgtaaaattg ggcttggctt gtctccatat
     9181 aatcctgcac ctaaaattat ttcatattct acatccttaa gttgatttat atctttaaaa
     9241 ttcatcattt tatatgtcaa aaaaacttca aaaataagga agactaggat aggtagccct
     9301 attaaaatag atattctagc taaaattttc ttctttagca aataatcact tcctatgttt
     9361 attttttaaa ttaatataaa ttttatcctt aagcttatta tctcatatat aacttacata
     9421 attatgattt aaatgttaag gaactattat aataatatgt aggtaaaaat tggagttgtg
     9481 aaatttttga taattatatt gactaggcag aatctgatga tataataaat agtgtttata
     9541 tgaaaatgat tatcattaat gagggtcgag ggtaatctaa taaggaaatt aggaggacta
     9601 aagatgaaag gacttaaaaa attagcgatt ttagggatcg caacacttac ggttggaatg
     9661 tttgtaggat gttctagtag taaaactagt gaagagagca acactagtaa tgaaaaaagg
     9721 attgttcaat ctgtaaaagg agaagttgag ataccagcta atcctaaaaa gatagtagac
     9781 atttcaggaa gttcagaaga gttagtttta ttaggacata ctcctgtagc tacggcaaat
     9841 gtagactcat ataaaactga tcaagtacca gaatatatgg ctgagacgtt aaaagataca
     9901 aaaatagttg gtcattctat gatggatact gctgatatag aagggatatt atcagtagag
     9961 ccagacttaa taataatgtc acctagacaa gagaaaatat atgatcaatt agcagaaata
    10021 gctccaacag ttataattaa tgataatgcg aatgattggg aagctaagtt taaagaggta
    10081 gcaaaattat ttgatcaaga agaggaagca caaaattggt tagataatta ttataaaaaa
    10141 gcaaaagaag ttggagagga aataaagaaa gtaaatggtg atggaactta tttatctgtt
    10201 ttagcttctt caggtcaatt catggtattt actgaaggtg gaataggaac agttttaaag
    10261 agtgatatgg gattacctca accaagtaaa atgccagaac aagatagcat aacattacct
    10321 acagttacac ttgaaggact tactgaaata gatgcagatc atattatagt tttaggaacg
    10381 gaatcagata aggctgattt agagtcaaat agtgtttgga atgaaatgag agctgttaag
    10441 aatggaaatg taaaaatact tgatgtaagt ccatatttta gccaagctta taacccaatg
    10501 ggaagagaac ttttattaga gtcaataaaa aatgaacttg taaaataatt aaaattttta
    10561 gtaggggctg tatcaaaata taaagataca gccccaatat ttaagttaaa atagaaattt
    10621 tggtgatact ttaaggagga caacttatga aaataggaag gaaaaagacc ttgacttatg
    10681 gattaatgtt tataggttta ttacttttgc ttagtggaat cattctatct atatctatag
    10741 gggcaaaaga aattgatttt tctatgataa taaatgctat ttttacaaat gatgcagata
    10801 taaatactaa tataattagg gatgtaagaa tacctagagc acttgcagca gctttagtag
    10861 gaggattttt agccgtagct ggtgccataa tgcaaggaat aactagaaat cccattgcag
    10921 aaccttctgt aatgggtata actcaaggag ctacatttat gatagctgta gcatttgttt
    10981 tacaaagaat atatcctaat ttagtcataa atagctttgc tcttatgatt tttgcatttt
    11041 taggggcaag tataagtggt cttttagtat attttataag ctctagagca tctagaaaag
    11101 ttgatcctgt taagttagcc cttgcaggga cagcattagg aactttactt atatccttag
    11161 caatgggaat atcaatgtat tttaatttat ctcaacagtt aagtttctgg gtatcaggtg
    11221 gattagtaag tgctaaatgg gaaggggtaa ttttattagc tttaattgga ggaatagcat
    11281 ttattggggc catgattatg tcacctagaa taacaatttt gagtttagga gaagaagtag
    11341 ccataggact tggagaaaaa accaatcttg taaggtttat atccataatg atagttatat
    11401 gtctaacagg tgcttctgtt tcggtagctg gtaatattgt ctttgtagga ttaataattc
    11461 ctcaaatatg caaaggaata gtagggtctg attatagata tataatacct agttcaatga
    11521 tattaggtgc tgttttatta gtgtatagtg atatattagc aaggattata aaccctcctt
    11581 atgaaacacc aataggatct ttaactgcct tattaggagt tccagtgttt atatacttag
    11641 ttagaaaggg aggtaagtaa tttatgaata taaaaaagaa ccataaattt atatgggtaa
    11701 cgggaatttt aataggttta atttttatta ccttcttaat aagtttaaac atgggttcac
    11761 tttcaattga gccaggagaa gttattaaaa ctttaattgg acaaggtaca aaaagtaatg
    11821 aaatagctat atttaagctt agacttccta gaatagtcat aggaatatta gtaggaacag
    11881 ctttagcaac tgcaggtaca atcctgcaag gggttactaa aaatgattta gctgattcag
    11941 gaatacttgg aataaattca ggagcggcct tatttattgt agtttatata tatataatga
    12001 atggaaatgt atatgatggt gtaagtaatt taactatttt tactatgcct atagtagctt
    12061 taagtggagc tatatttgga gcatttttaa tatacatatt agcatggaag aatggaataa
    12121 attcatcaag actattatta ataggtatag gtataaatat agcttttacc tccttattaa
    12181 caatatttca gttaagattt actactcaag aatttaatag agttatggct tggatatcag
    12241 gaagcatatg gggagcaagt tggaaatatg tattagcaat attaccattt ataattatat
    12301 ttatggcttt aactatatat aaatcaagat atttagatgg attaaattta ggagacgaag
    12361 ttgccacagg tatagggatt gaagtagaaa aagaaagaag aaaactaatt atatatgcag
    12421 ttatattagc aggggtagct acatcagttg caggtagcat aggtttccta ggtttagtat
    12481 ctcctcatat agcaagaaaa ttaatagggc caaagcataa gaaattaatt cctgtagcag
    12541 cccttattgg aagtttaata ttattagtgt cagatactat agcaagaaat ttaattgcac
    12601 caatggaaat gccagtaggt ataatcgtat ctataatagg agttccatat tttatttatt
    12661 taatgttatc aaaatagata tttataaata aaaacgaaag tagagggaga taattatgaa
    12721 ttgtatagct actgataatt taaatatttc atatggaagt ttagatatag ttaaaaattt
    12781 aaacttaaat ataccaaagg ggaaaataac tacaataata ggtgcaaatg gatgtggaaa
    12841 atctacagtg ctaaaaacaa tagctagaat tttaactcct aaaagtgggg aaatatatat
    12901 aaatgaaaaa aacataaaag agcagggagc taaggagtta gcaaaggtta tggctgtttt
    12961 acctcaaagt cctcaagcac ctaatgggct tactgttgaa gagttaatag cctatggaag
    13021 attccctcat caaaaaggat tcggtaaatt aaaaaaagag gacaaaaata taattcaatg
    13081 ggctttagag gttacaggat taattgaatt tagggaaaga gacatagaag atttatctgg
    13141 agggcaaaga caaagagctt ggattgctat ggccttagca caacaaacag atatattaat
    13201 ccttgatgag ccaacaactt atttagattt agcccatcaa ttagagattt taaatttact
    13261 taaggaattg aatgagaatc atgggacaac tatagttatg gttattcatg aacttaataa
    13321 tgctgctaga tttgccgatt atatgatagg tatgaaagca ggaaaaatag cttgccaagg
    13381 tacagcatac gaagttatga caaaagaaaa tcttaaagag ctgtttaata tagatgcaga
    13441 aattatagag gatccaagaa ataaaagacc agtctgtgta acttatgata tgttgtaaaa
    13501 ttaagatgat aatttttaaa atataaatgt aattctaata atagtataag tatagtttaa
    13561 taaaaataaa tcttagagaa tattttattt tctaagattt attttattta ttaaagtttt
    13621 tatgattaat gaaatactta atatgatata ataatattta attgggttaa attggaattt
    13681 aaataaagtg taatatctat aaatgtagat atattaaaaa gtatatttat taattagtat
    13741 gaaataaagg tataagtata ttaattaatt tttaagttga tttagtataa aaagtagaga
    13801 ggataattta tgagtttaag gattagagat attaagatag aagattataa agaaattagt
    13861 aaaattagaa aaatgcctgg agttatggag aatatacttt ccaataagga tgaggaagag
    13921 gaattaatta aggaaaagat tataaatagg ggcaagaatc agtattggta tgttgctgaa
    13981 gaaaatggaa aagttatagg attaggaata ttaatgaatc atggcaattt aagaaagaaa
    14041 catgttggag taataacact aatggtcaat agtgattatc aaaataaagg tgtaggaaat
    14101 ttattaatgg ataagttaat aaatttaagc gaaagtctta atattataag gttggaatta
    14161 tgtgtattta gggataatta taaagcaata aacttatata agaagtttga atttaaagaa
    14221 gagggaataa aggaaagtca gctttgaaaa atggagaata tgctgatgaa attatgatgg
    14281 taaggataag cttgtagagt taatttaggg ataatatata tttataattt taaatagtat
    14341 aaataggttt agagaataaa gggggagagg ttatatgtat gaatataaat ttataaatgt
    14401 gcctgttgac aaatcattta agtgcaaaag gggagactcc tttgaaaaat gtaaagatat
    14461 tataaaagaa gaagttaaaa atggatggag gttaaagcag attgttactc caattaatga
    14521 aaagtcaggt gttaatagta cctatgctta tgaaataatc tttgaaagaa aagtagaaaa
    14581 ttatgcttaa taaagaactt tttaataaga gaaatttaag gactataatt ttagctatgg
    14641 ttctttttat tccttttata tggggaacca aattagcttc aatatggttc agtgatatgg
    14701 gaaaagaaaa aatatctatt attaagaaaa taggaatttc atttttaata ttaggacctt
    14761 attggggggt ggctataagt ggattttata aaagattaag aagaatatta aattacaaat
    14821 tattaatttg atgttcttta ttatatattt ttattatttc tttcttatag atagtattgt
    14881 aaataattta attaagtaga ttgtaattaa aaatatagat agttatatat taataaagtt
    14941 attggtgaag tttattaaat aaaacattta aagattgtat tactatattc tttgtaggta
    15001 attacattat agaaacttat ttgatattaa tataaattat atgaaatcta gatggataac
    15061 aaaaatagtt gctaaataaa agcaactatt tttgcttatt taaatattac ttaacttatt
    15121 tttttattga tttcaaacat ttctgataca tagtatataa agcaacctac aagaggtatt
    15181 aataaataag catcttcaaa tttaaaaaca aaaaatacac actctgttaa aactaaaatg
    15241 gtaccaataa ttataaatgt tatacctatt aagtctgcaa tatgatcttt attttttatt
    15301 tggttcatat catagtagtt ttcaattctt ttgaagtata aaagataata tgcaagtt
//
LOCUS       DAEVGI010000051        14786 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEVGI010000051 DAEVGI010000000
VERSION     DAEVGI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14786
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.051"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..169)
                     /locus_tag="K8M77_001320"
     CDS             complement(<1..169)
                     /locus_tag="K8M77_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase"
                     /protein_id="HBI7085821.1"
                     /translation="MTNKMKNHKITKLGGLNNSNYLLECENNKYVLRIPSKDNKNNFS
                     EENFVLIFANLN"
     gene            234..1319
                     /gene="cobT"
                     /locus_tag="K8M77_001321"
     CDS             234..1319
                     /gene="cobT"
                     /locus_tag="K8M77_001321"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBI7085822.1"
                     /translation="MLNEVLKGIQGLDNDMILKAKNRVDSLAKPLGSLGKLEDIAIRL
                     SGITGNMFNNIDKKCVIIMSSDNGVEEEGVASAPQCLTLLQTKNFIKGINGVGTLAKT
                     NGTDLIVFDVGINSDEVIEGVIDRKISKGTKNIYKEPAMTYEEAKKSLEIGIEAVKIA
                     KEKGYKILGVGEMGIGNTTTSAAVLKALIGCETSKVVGKGGGINNASFEKKKIIVEEV
                     VKKHNIDFDDPIDIISKVGGYDIGAMAGVFLGAAFYRIPVVIDGFISVVAALLANRLN
                     PLVKEFCFTSHKSQEIGYDLAIKELGLDPILDLNMRLGEGSGCPIAFSVINFAISMMN
                     NMATFEEVNIDNSYLEDVKDKECYVVL"
     gene            1358..1918
                     /locus_tag="K8M77_001322"
     CDS             1358..1918
                     /locus_tag="K8M77_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBI7085823.1"
                     /translation="MIVLIIGGAKSSKSSYAEFYSSSLSKKGKGELYYLATMKPYDLE
                     DLKRIENHLKSREKYDFKTIEKHRNLNEIFHKLSKEDTVLLDSLTSLVTNEMFVGKEF
                     NESVHSNILKDIKRLGTKVSNLVIVSDYVFSDGIKYDSYTESFKRELGKINCELASFS
                     DVVIEASFGNLIFHKGEEVLEDENFL"
     gene            1902..2657
                     /locus_tag="K8M77_001323"
     CDS             1902..2657
                     /locus_tag="K8M77_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBI7085824.1"
                     /translation="MKIFYKAINMTLSMFTVIPLPKYEWDDRAAKHIMKLYPFIGLII
                     GILWYLSFFVLSKLNVPIMLMAALILTVPYILTGFLHLDGFMDVSDALLSRRDKETKL
                     RILKDSTVGAFSVISVVLLLLVEFAGIFTVLNKNLDMRILIFIPIASRTMNGYFIVSQ
                     EMLGQSSLAKFFKEGTGKVDEIILLGIYVLVALITFFTLGINYLIAILAMGLISFILL
                     LKVKKELGGINGDVAGYILVLMEFTGILLLGII"
     gene            2671..3051
                     /locus_tag="K8M77_001324"
     CDS             2671..3051
                     /locus_tag="K8M77_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBI7085825.1"
                     /translation="MKLLFGGAYNGKLRYVREKLGINEEEIFYCKEDSIDFSKKVISG
                     IDRLIYFNALQGKESLSFFKEKKELLKDKILICDEISSGIVPLKKEERLWREETGKVL
                     QFLSKESEEVYRIFFGIGTRLKGE"
     gene            3056..3700
                     /locus_tag="K8M77_001325"
     CDS             3056..3700
                     /locus_tag="K8M77_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI7085826.1"
                     /translation="MIIYLIRHGRTYCNENKLYCGISDVPLSEEGKKELEEKRKSVRL
                     PICKRNFTSGAKRANETFNILYPNKKYEVIKEFSEYNFGDFELKSYEMLKEDKEYLNW
                     ILDKSKEVSCPNGESRRDFEKRMKKAFLKLLLTLKEEGEEEALIVCHGGSIACILELF
                     EKDRLDFYTYQPKCGGGYKLKVEQDINTLKGDFSSLECEKDITDNLRLEIIERF"
     gene            3703..4650
                     /locus_tag="K8M77_001326"
     CDS             3703..4650
                     /locus_tag="K8M77_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBI7085827.1"
                     /translation="MIKIIIGYVLDLIIGDPNNPYHPIRYIGKLASNMEKLWRKVFKK
                     NLKIAGFFAWIFIVFITFGVTLGILHIANKINPILGTVVSGILIYFCISAKGLKVEGL
                     KVIKILKEGDIVKARKQLSYIVGRDTENLDEEAIVRAVVETVAENMSDGIIAPLFFAG
                     IGGAPLAFLYKAVNTCDSMFGYKNEKYKDFGFFSAKLDDVFNYIPARLTAYLIVISSF
                     ILRLNLKDSFKIYKRDRYNHSSPNSAHPEAAVAGALGIRLGGANYYFGKLVEKPTIGD
                     AKKKIEISDVYKTNNILGMVSFLGMVVALIIRCILEVII"
     gene            4647..5711
                     /locus_tag="K8M77_001327"
     CDS             4647..5711
                     /locus_tag="K8M77_001327"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase"
                     /protein_id="HBI7085828.1"
                     /translation="MNLGHGGNVEEIKRIYGLKENEIIDFSANINPIGISERVKEAMV
                     RGINSIERYPDITYYELKKGISNFEKVALENIVLGNGAAEVIFNIVRGIKPKKALIVS
                     PTFSEYEDALNSVECEVNHYILKDNYSVDNGFLEEIKEELDIIFLCNPNNPTGALIEK
                     DFSLKVLEKAKKMNITVVFDESFLDFVEDNYKYSLIKELDNFQNLIVVKSLTKLFAFP
                     GIRLGYGLSSNNNFIEKINLVGTPWSVNTVADYAGREALKDVEYIKNSVAYIKKENEF
                     LYNGLENFKDIITFKGAVNFIFFKLNKNINLREELIRRGIIIRSCSNYIGLDHSFYRV
                     AVRTREENKKLLEELSKVLK"
     regulatory      5796..5973
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6269..7186
                     /locus_tag="K8M77_001328"
     CDS             6269..7186
                     /locus_tag="K8M77_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI7085829.1"
                     /translation="MRNFKKVISLVMITMMALLMFTGCGSKTVTMKDMEGTEFTVPKK
                     IERIVSTAPSNTEILVGLGLGDKIVGVDKYSSDVKGLNSDIPKIDFRNPDAETIVALK
                     PDVIIASGHNREGKEDPFKALKDAGIPVVYIPTSDSIDGIYEDINFLAKLTGTEAEGK
                     KMVDSMKSEVDKIKAIGENIKDKKTVYFEIGSGSKLYSFGSDTFLNNLLTIIGAENIF
                     GNEKGWISPSEEAVIKANPDVILTNESYIENATDLIAKRPGWNTITAVKDNKIFLVDN
                     NASSRGSQYVVNALKQMAKDIYPEQYAGI"
     gene            7173..8204
                     /locus_tag="K8M77_001329"
     CDS             7173..8204
                     /locus_tag="K8M77_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI7085830.1"
                     /translation="MQESKKRKLLILLAIPFVILVLCIGTSIGSSNINIWDTISIVGN
                     KIFNIPLRAGIKPEDVAIIWVIRLPRVLLAFGVGASLAVSGAIIQSILKNPLASPYTL
                     GVSSGASLGVGIYIVLGISIPVVGNLAMPIIGFLCGVLTVFAVIIFSNKVDRGLSNNT
                     VILAGMVLSLFTSAMLTTITALCSEDIKAITMWQMGSFAMKSWSYVFAGIPFFIIGIF
                     MAVRYTREMDILSFGEDGAKAIGVETKKVKKTLLVSVAILTGSAVALSGVIGFIDLIT
                     PHIVRKIFGSNHKIVIPMCIILGGTLMVLTDLVSRTLISPSELPVGAITALIGAPFFA
                     YLYFGKRSR"
     gene            8209..8964
                     /locus_tag="K8M77_001330"
     CDS             8209..8964
                     /locus_tag="K8M77_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085831.1"
                     /translation="MLEVKNLCCGYDGNEVIHNLSFNINRGENISIIGPNGCGKSTVL
                     KALANLIEYKGEIKLDGVEIRNIKRKDLAKKVALMSQNSEVYFSYTVFETVSLGRYAH
                     MDGIFSSLTKKDRDIVLKCIESVGLLNEKDRLINELSGGQLQRVYLARAFAQDPEILL
                     LDEPTNHLDLKCQIEILDYINKWTKENNKTVIGVLHDLNLVQMFSEDAILIKEGKIYS
                     SGKARELLNSSKITDVYKIDIRKFMLEALEKWK"
     gene            8974..9627
                     /locus_tag="K8M77_001331"
     CDS             8974..9627
                     /locus_tag="K8M77_001331"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBI7085832.1"
                     /translation="MKKKFVVSYSCGKDSTLSLYRMIKAGHTPVALLVTVDKKVLRSW
                     FHGIPDKLLKDVSKSLDIPLVLVASNGKENYGETFVEALKKIKEEMGAEACVFGDIDL
                     MAHRTWCEDKCEKAGLEAIFPLWEEDREALTYEFIDSGFKTVIKNVKLSILGEEFLGE
                     VLTKDVVERLKAAGSDACGENGEYHTFVFDGPLFKERINFETGDNILTETHGFLDIK"
     gene            9637..11100
                     /locus_tag="K8M77_001332"
     CDS             9637..11100
                     /locus_tag="K8M77_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBI7085833.1"
                     /translation="MKYKSIMLLGTASSVGKSTVAAAFCRYFKKKGYRVAPYKALNIS
                     LNSFVTKDGDEIGRAQVVQAEACEIDPKEYMNPILMKPSAGFKTQVIVRGKVHCTMDA
                     YKYKELNKYLKEKAKEAYDDISNDYDLIVLEGSGSCAEINLRETDIANMHTAKMADAD
                     VILVSDINRGGVFASIVGTIMLLTEEERKRVKGVIINKFRGKREFFEPAMRQIEEIIK
                     IPVLGVMPYFDLDIEEEDSASIKLRKGNGKGIDIAIVRLPHMSNFTDFNSLGRIKDVC
                     IRYAENPKDLENANMIIIPGSKNTIDDLIYLKESGFKEALINESSKGKLIFGICGGYQ
                     ILGEKIIDSLGVEGDIREEEGLGLLNIVTSFNKEKTTKQVVAFDLEGNEVSGYEIHNG
                     ESVPTAKENIWIKEQNGNVLGMKNKEQNVFGTYIHGIFDEGDFGEKLINKLKKELNIE
                     ESNDVNYKDYKMSQYDKLCELLEENIDMAYVENLIRY"
     gene            11407..13125
                     /gene="hcp"
                     /locus_tag="K8M77_001333"
                     /gene_synonym="priS"
     CDS             11407..13125
                     /gene="hcp"
                     /locus_tag="K8M77_001333"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI7085834.1"
                     /translation="MENKMFCFQCQETAGCTGCTKFGVCGKSPDLARMQDLLIYTTKG
                     LSEVTTRLREEGNKIDSKVNHLVTINLFTTITNANFDDEIFYERVKETLRVKESLLNT
                     LKNKKNLSEAALWNASNKNEIDEKVSLLQKIKNAFSKKEEISIDSILDEKSTKVGVLA
                     TKDEDIRSLRELIIYGLKGMSAYMKHANALGYDKEEINAFMQSTLAKTLDDSLTIDEL
                     IALTLETGKVGVDGMALLDSANTGTYGHPEVTKVNIGVRKNPGILVSGHDLKDLEMLL
                     KQTEGTGVDVYTHSEMLPAHYYPAFKKYSHFAGNYGNAWWKQKEEFESFNGPILMTTN
                     CIVPPKDSYKDRLYTTGAAGFAGCTHIKGNSESDKDFSVIIEHAKKCKAPKQIETGEI
                     IGGFAHNQVLALADKVVEAVKSGAIKRFFVMAGCDGRAKSRNYYTDFAKALPKDTVIL
                     TAGCAKYKYNKLPLGDINGIPRVLDAGQCNDSYSLALIALKLKEVFELEDINDLPISF
                     NIAWYEQKAVIVLLSLLYLGVKNIHLGPTLPAFLSPNVAKVLVENFGIGGITNVEDDI
                     KMFLNN"
     gene            13640..>14786
                     /locus_tag="K8M77_001334"
     CDS             13640..>14786
                     /locus_tag="K8M77_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBI7085835.1"
                     /translation="MDDLIKAIDKITEEEIIKLVISNKLNKDTEYKKIVFQLKEKNKK
                     DFYQIEKYTEKQVFHENIEKKELKEKIEELFVSKYKQLNAFSKESSFDLKVSKKGKVF
                     LGIKKNNEALKILKKEHNRKKNYILEEGMIIEPLIDLGVFTKEGKVINSKYDKYKQIN
                     RFIEIIDDEIKKIDQKELTILDFGCGKSYLTFILYYYFVEIKKIKVNMIGLDLKADVI
                     KKCNEIAKKYNYENLHFELGDINGFKYNNKVDMVITLHACDTATDYALYNAIKWNSKM
                     IFSVPCCQHEFNSQIESEKFSILTNYGIIKERISALMTDSVRANLLECAGYKTQLLEF
                     IDIAHSPKNILIRATKGNVSKGKKEKSLNEVNNLMSEFNLSPTLYKLL"
BASE COUNT         5803 a         1398 c         2754 g         4831 t
ORIGIN      
        1 tgttcaaatt agcaaatatt aaaacaaaat tttcctctga aaaattattt ttattatctt
       61 tactaggtat tcttaaaaca tatttattat tttcacactc taataaataa tttgagttat
      121 taagaccgcc tagtttggtt atcttatgat ttttcatttt gttagtcatc ttaatctcct
      181 tatattctaa tgaaaatatt atatcaaaaa ataagaaggg gtggaaaagg ataatgttaa
      241 atgaggtgtt aaaaggaata caaggtttag ataatgacat gattttaaaa gctaaaaata
      301 gagtggattc tttagctaaa cctttaggaa gtttaggaaa gttagaagat atagctataa
      361 ggttatctgg aataactgga aatatgttta ataatattga taaaaagtgt gtaattataa
      421 tgtcttctga caatggagtt gaagaagaag gcgtagcctc agcgcctcaa tgtttaactt
      481 tacttcaaac aaaaaatttt ataaaaggta ttaatggagt aggtacattg gcaaaaacta
      541 atggaacaga tttaatagtt tttgatgtag gaattaattc agatgaagtt atagaaggag
      601 ttatagatag aaagatatca aagggaacta aaaatattta taaggaacca gctatgactt
      661 atgaagaagc taaaaaatcc ttagaaattg gaatagaagc tgtaaaaata gctaaggaaa
      721 agggatataa aatacttgga gtaggtgaaa tgggaatagg aaataccact acttcagcag
      781 cagtattaaa agcccttatt gggtgtgaaa cttctaaagt cgtaggaaaa ggtggcggaa
      841 taaataatgc ttcttttgaa aagaaaaaaa taattgtaga agaagtggtt aaaaaacata
      901 atattgattt tgatgatcct attgatatta tttcaaaggt tggaggatat gatattggag
      961 caatggcagg ggtattttta ggtgctgctt tctatagaat ccctgtagta atagatgggt
     1021 ttatatctgt tgtggcagct cttttagcta atagattaaa tcctttagtt aaagaatttt
     1081 gttttacatc tcataagtca caagaaatag gatatgattt agctataaaa gagttaggat
     1141 tagatcctat attagattta aatatgagat taggagaagg aagtggatgc ccaatagctt
     1201 tctcagtaat taattttgca atatccatga tgaataatat ggcaactttt gaagaagtaa
     1261 atatagataa tagttattta gaggatgtaa aggataaaga gtgttacgtt gttctttaaa
     1321 actttaaaaa atattatagg agttaatttt attagttatg atagttttaa ttattggagg
     1381 cgctaaaagt agtaaatctt cttatgctga attttatagt agtagtcttt caaaaaaagg
     1441 aaaaggggaa ctttattact tagctactat gaaaccttat gatttagagg atttaaagag
     1501 aatagaaaat cacttgaaaa gtagagaaaa gtatgatttt aaaacaattg aaaaacatag
     1561 gaatttaaat gagatatttc ataaactttc taaggaagat actgttttac tagacagttt
     1621 aacctcttta gttactaatg agatgtttgt aggaaaagaa tttaatgaaa gtgtacatag
     1681 taatatttta aaggacataa agagactagg aactaaggtt tctaatttag taatagtttc
     1741 agattatgtt tttagtgatg gaattaaata tgattcttat actgagtctt ttaaaagaga
     1801 attaggtaaa ataaattgtg aattagcttc cttctcagat gtggttatag aagcttcctt
     1861 tggaaactta atatttcata aaggagagga ggttttagaa gatgaaaatt ttttataagg
     1921 ctataaatat gaccttaagc atgtttacag ttattccatt gcctaaatat gaatgggatg
     1981 acagggctgc aaaacatata atgaagcttt atccttttat agggttaatt ataggaatac
     2041 tttggtattt aagttttttt gtattaagta agctaaatgt accaattatg cttatggcag
     2101 ctcttatttt aacggttcca tatattttaa ctggattctt acatttagat ggttttatgg
     2161 atgtttctga cgcacttctt tctagaagag ataaggagac aaaacttaga attttaaagg
     2221 attctacagt tggagctttt tcagttattt ctgtagtttt attactacta gtagaatttg
     2281 ctggaatttt tactgtttta aataaaaatt tagatatgag aatattaata tttataccaa
     2341 tagcatcaag gacaatgaat ggatatttta ttgtaagtca agagatgcta ggacaaagtt
     2401 ctctagcaaa attctttaaa gaaggaactg gaaaagtaga tgaaataatt ttactaggaa
     2461 tatatgtatt agttgcatta ataacgtttt tcaccttagg aataaattat ttaatagcta
     2521 ttttagcaat gggacttatt tcttttattt tacttttaaa agtaaaaaaa gaattaggag
     2581 gaataaatgg agacgttgca ggatacattc ttgttcttat ggagtttaca ggaattttac
     2641 ttttaggaat tatttaagga ggagaaactt ttgaaacttt tatttggcgg tgcctataat
     2701 ggaaagctaa ggtatgtaag agaaaagtta ggtataaatg aagaggaaat attttattgc
     2761 aaagaagatt ctatagactt ttctaaaaag gttattagtg gcatagatag attaatttat
     2821 tttaatgcct tacaaggcaa agagtcttta agctttttta aggaaaaaaa agaattatta
     2881 aaagataaaa ttttaatttg tgatgaaata tcttcaggaa tagttccttt aaagaaagag
     2941 gaaagattat ggagagaaga gactggaaaa gttcttcagt ttttatcaaa ggaaagtgaa
     3001 gaagtttata gaatattttt tggaatagga acaagattaa agggtgaata ataagatgat
     3061 aatttattta attagacatg ggaggactta ttgcaatgag aataaacttt attgtggaat
     3121 aagtgatgtg cctttaagtg aggaaggaaa aaaagagtta gaggaaaaaa gaaagagtgt
     3181 gagattgcca atatgtaaga gaaattttac aagtggagca aaaagagcta atgaaacatt
     3241 taatatttta tatccaaata agaagtatga agtcataaag gaatttagtg agtataattt
     3301 tggagacttt gaactaaagt cttatgaaat gctaaaggaa gacaaggaat atttaaactg
     3361 gattttagac aaaagcaagg aagtttcttg tcctaatggt gaatcaagaa gggattttga
     3421 aaagagaatg aaaaaagcat ttttaaaatt acttctaacc cttaaggaag agggagaaga
     3481 ggaagcctta atagtatgtc atggaggaag tatagcttgt attttagaac tctttgaaaa
     3541 ggatagatta gacttctata cttatcagcc taagtgtgga ggaggatata agttaaaagt
     3601 tgaacaggat atcaatacct taaaaggaga tttttcaagt ctagagtgtg aaaaagacat
     3661 aacagataat ttaagactag aaataataga aaggttttag aaatgataaa aattataata
     3721 ggatatgttt tagacctaat aataggagat cctaataatc catatcatcc tattagatat
     3781 ataggaaaac tagcatctaa tatggaaaag ctttggagaa aggttttcaa gaaaaatctt
     3841 aagattgcag gcttctttgc ttggattttt atagttttca taacctttgg agtaacttta
     3901 ggaattttgc atattgcgaa taagattaat cctattttag gaactgtagt atctggaata
     3961 cttatttatt tttgtatttc agctaagggg cttaaagttg aaggacttaa agtaattaag
     4021 attttaaaag aaggagatat tgttaaggct agaaaacaat tatcttatat tgtaggaaga
     4081 gatactgaaa acctagatga agaagctata gttagagctg tagtggaaac tgtagcagaa
     4141 aatatgtcag atggtattat agcaccttta ttttttgcag gaattggggg agcaccttta
     4201 gctttcttat ataaagcagt aaacacatgt gattcaatgt ttgggtataa aaatgaaaaa
     4261 tataaggatt ttggattctt ctcagctaag cttgatgatg tgtttaatta tattccagct
     4321 agattaacag cttatcttat agtaatttct tcttttattt taagacttaa tttaaaagac
     4381 agttttaaaa tatacaaaag agatagatat aatcattcaa gtcctaacag tgctcatcca
     4441 gaagcagcag ttgcaggggc tttaggaata agacttggag gagcaaatta ctattttgga
     4501 aagcttgtag aaaaaccaac cataggagat gctaaaaaga aaatagaaat ttcagatgtt
     4561 tataagacta ataatatatt aggaatggtt tcttttttag gaatggtagt agcacttatt
     4621 attagatgta ttttagaggt gattatatga atttaggaca tggtggtaat gtagaagaaa
     4681 ttaagaggat ttatggctta aaagaaaatg aaattattga tttttcagct aatataaacc
     4741 ctataggaat ctctgaaaga gtaaaggaag ctatggtaag aggaataaat tctatagaga
     4801 gatatccaga tataacttat tatgagctta aaaaggggat ttcaaacttt gaaaaggtag
     4861 ctttagaaaa tatagttcta ggaaatggtg cggcagaggt tatttttaat atagtaagag
     4921 gaataaaacc taaaaaggct cttatagtat caccaacctt ttcagagtat gaggatgctt
     4981 taaattctgt tgagtgtgaa gttaaccatt atatattaaa agataattat tcagtagata
     5041 atggattttt agaagaaatc aaggaagaat tagatattat atttttatgt aatcccaata
     5101 atcctacagg tgctttaata gaaaaagatt tttcattaaa agtattagaa aaagcaaaaa
     5161 aaatgaatat tacagttgtt tttgatgaat cttttttaga ctttgttgaa gataattata
     5221 aatactctct tattaaagaa cttgataatt tccaaaattt aattgtagta aaatctttaa
     5281 ctaaattatt tgcctttcct ggaataagat taggttatgg attaagttct aataataatt
     5341 ttatagaaaa aataaattta gtaggaactc cttggagtgt aaataccgta gcagactatg
     5401 caggaagaga agcattaaaa gatgttgagt atattaaaaa cagcgtagct tacataaaaa
     5461 aagaaaatga atttttatat aatggcctag agaattttaa agatattatt acttttaaag
     5521 gtgcagtaaa ttttatattt tttaaattga ataaaaatat aaatttaaga gaagaattaa
     5581 ttagaagagg tataataata agaagttgta gtaattatat tggcttagac catagcttct
     5641 atagagtggc agttagaaca agggaagaaa acaaaaagct tttagaagaa ctttcaaagg
     5701 ttttaaaata agccttatac tattactttt attattaata ggttttatat taaattaaga
     5761 agtttgacaa tgataaaata taataatata atcaagttga ttaactaata attggtgtga
     5821 ttttcgctta atagggaatg aagttaaagt cttcaactac ctcagtaacc gtgaagcaga
     5881 caaaatctca atatgtcact gcatttttgt gcgggaagac gagatggagg aagaagcaaa
     5941 gtcgggatac ctgcctttta tttaagtgct attgtcttct gagggtagga taataggtgc
     6001 gtatgattta aatatgctta acataccttc ttaaaatggt atgttttttt attttatacg
     6061 tattaattta agcctcaaat attttaattg ttaagtttat gaaagggaaa tttgatttgt
     6121 gatgatttag gaaagttcag atttgctttt atagattgta aggggtactt agcaatatta
     6181 aaaaataaat attaaaatca ttaaataaaa tcaaatagga atccttcata taacttaaaa
     6241 tttataaagg tgaaagtgga ggaataatat gagaaatttt aaaaaagtaa tttctttagt
     6301 aatgataacc atgatggcat tacttatgtt tactggatgt ggaagtaaaa cagtaacaat
     6361 gaaagatatg gaaggaactg aatttacagt acctaaaaag attgaaagaa tagtatcaac
     6421 agctccgtct aatacagaga tattagtagg tttaggacta ggtgataaaa tagttggggt
     6481 tgataaatat tcatcagatg taaaaggatt aaattctgat attcctaaaa tagactttag
     6541 aaatccagat gcagagacta tagtagctct taagccagat gtaataatag cttcagggca
     6601 caatagagaa ggaaaagaag atccatttaa ggctttaaag gatgcaggaa tacctgtagt
     6661 ctatattcca actagtgata gtatagatgg aatatatgaa gatatcaatt tcttagctaa
     6721 gttaactgga acagaagctg aaggaaagaa aatggttgat tcaatgaaaa gtgaagtaga
     6781 taaaataaaa gctataggtg aaaatataaa agataagaaa actgtttact ttgaaattgg
     6841 atcaggttca aaactttata gctttggaag tgatactttc ttgaataatt tattaactat
     6901 aattggagca gaaaacatat ttggtaatga aaagggttgg atttctccaa gtgaagaagc
     6961 tgttataaaa gcaaacccag atgtgatttt aacaaatgaa agttatattg aaaatgctac
     7021 agatttaatt gctaaacgtc caggttggaa tacaataact gctgttaagg ataataaaat
     7081 attcttagta gataataatg catcatcacg tggaagtcaa tacgtggtaa atgcattaaa
     7141 acaaatggca aaggatattt atccagaaca atatgcagga atctaaaaaa agaaaactac
     7201 taatattact agccattcca ttcgtaatat tagtactctg tattggaact tccataggaa
     7261 gttccaatat taatatatgg gatactattt ctatagttgg gaataaaatt tttaatattc
     7321 ctcttagagc tggtataaaa ccagaggacg tagcaattat ttgggtaatt agattaccaa
     7381 gggtattatt agcttttgga gttggagctt cccttgcagt tagtggagcc ataattcaat
     7441 ctatattaaa aaatccatta gcatcaccat atactttagg agtttcatca ggagcttccc
     7501 taggagttgg aatatacata gttcttggaa tttcaattcc tgtagtagga aatttagcta
     7561 tgcctattat aggattttta tgtggagttt taactgtttt tgctgttatt atcttttcta
     7621 ataaagttga tagagggctt tcaaataata cagtaatatt agctggtatg gttttgtcac
     7681 tttttactag tgcaatgtta actactataa cagccttatg cagtgaagat ataaaggcaa
     7741 ttactatgtg gcaaatggga tcatttgcta tgaaaagttg gtcatatgtt tttgctggaa
     7801 ttccattttt cataattgga atttttatgg ctgtaagata tacaagagaa atggatatct
     7861 taagctttgg agaagatgga gcaaaagcaa taggagttga aactaaaaag gtgaaaaaga
     7921 cactacttgt ttcagtggca attttaactg gaagtgccgt agccttaagt ggtgttatag
     7981 gatttattga tcttataact cctcacatag ttagaaaaat atttggctct aatcataaga
     8041 tagtaattcc aatgtgtatt atacttggag gaactttaat ggtattaaca gatttagttt
     8101 caagaacact aatttctcca tcagaattac cagttggagc aattacagca ttaattggag
     8161 caccattttt tgcctatctt tattttggaa agagaagtag gtgatatttt gttagaagtt
     8221 aaaaatttat gttgtgggta tgatggtaat gaggttatac ataatttatc ttttaatata
     8281 aacagaggag aaaatatatc tataatagga ccaaatgggt gtggtaaatc tacagtgtta
     8341 aaagccttag ctaacttaat agaatacaaa ggtgaaataa agctagatgg ggtagaaatt
     8401 agaaatataa aaagaaaaga tttagctaaa aaagttgcat taatgagtca aaattctgaa
     8461 gtgtattttt cttatactgt ttttgaaaca gtttcactag gcagatatgc tcatatggat
     8521 ggtatttttt caagtcttac aaagaaagat agggatatag tcttgaagtg tattgaaagt
     8581 gtaggattat taaatgaaaa ggataggtta ataaatgaac tatcaggagg acaacttcag
     8641 agagtttatt tggcaagagc ctttgctcaa gatcctgaaa tattactttt agatgaacca
     8701 acaaatcatt tagacttaaa atgtcaaatt gaaattttag attatataaa taaatggaca
     8761 aaggaaaata acaaaactgt tataggcgtt ttacatgatt taaatttagt tcagatgttt
     8821 agtgaagatg caattttaat aaaagaaggt aaaatttata gtagtggaaa agcaagagaa
     8881 ttattaaata gttctaaaat tacagatgta tataaaatag acataagaaa attcatgcta
     8941 gaagcattag aaaaatggaa gtaggtggac aatatgaaaa aaaagtttgt tgtatcatat
     9001 agttgtggaa aggatagtac attatcatta tatagaatga taaaggctgg acatactcca
     9061 gtagctttat tagtcactgt tgataaaaaa gttttaagaa gttggttcca tggcatacca
     9121 gataaattat taaaagacgt atcaaaatct ctagatatac ctctagtttt agttgcaagt
     9181 aacggaaaag aaaattatgg tgaaacattt gttgaagctt taaagaaaat taaagaagaa
     9241 atgggtgcag aagcttgtgt atttggagat attgatttaa tggctcatag aacttggtgt
     9301 gaagataaat gtgaaaaagc aggacttgaa gctattttcc cactttggga agaagataga
     9361 gaagctctaa cttatgagtt tatagatagt ggcttcaaaa ctgtaattaa aaatgtaaaa
     9421 ttaagcattt taggagaaga attcttaggt gaagttttaa ctaaggatgt agttgaaaga
     9481 ttaaaagcag ctggttcaga tgcttgtgga gaaaatggag aatatcatac atttgttttt
     9541 gatggacctt tatttaaaga aagaataaac tttgaaactg gagataatat attaacagaa
     9601 actcatggat tcttagatat taagtaggtg tgttttatga agtataaaag tataatgctt
     9661 cttggtacag catcttccgt aggaaaaagt actgttgcag cagccttttg tagatatttt
     9721 aagaaaaaag gatatagggt tgcaccttat aaagctttga atatatcatt aaattccttt
     9781 gtaacaaagg atggggatga aataggacga gctcaagtgg tacaagctga agcctgtgaa
     9841 attgatccta aggaatatat gaatcctata ttgatgaaac ctagtgcagg atttaaaact
     9901 caggtaatag ttagaggtaa ggttcattgc actatggatg cttataaata taaggaatta
     9961 aataaatatc ttaaagaaaa agcaaaagaa gcttatgatg acatttcaaa tgattatgat
    10021 ttaatagtct tagaagggtc aggaagctgt gctgaaataa acttaagaga aactgatata
    10081 gcaaatatgc atactgctaa aatggcagac gccgatgtta ttttagtttc ggatataaac
    10141 agaggaggag tttttgcttc tattgttggt actataatgc ttttaactga agaagagaga
    10201 aaaagggtta aaggggttat aataaataaa tttaggggaa aaagagagtt ttttgaacct
    10261 gcaatgagac aaatagaaga aataataaaa atccctgtct taggagttat gccttacttt
    10321 gatttagata tagaagagga agatagtgct tctattaaat taagaaaagg taatggtaaa
    10381 gggatagata tagcaatagt tagattacct catatgtcaa actttactga ttttaattcc
    10441 ttaggaagaa taaaagatgt atgtataaga tatgctgaga atcctaagga tttagaaaat
    10501 gcaaatatga taataattcc aggaagtaaa aataccatag atgatttaat ttatttaaag
    10561 gaaagtggtt ttaaagaggc actaattaat gaaagttcta aggggaaatt aatatttggt
    10621 atatgtggag gttatcaaat attaggagaa aaaataattg attctcttgg tgtggaaggt
    10681 gacataagag aagaagaagg cttaggactt ttaaatatag taacatcatt taataaagaa
    10741 aaaactacaa agcaggttgt agcctttgat ttagaaggaa atgaagttag tggatatgaa
    10801 attcacaatg gggaaagtgt tcctacagct aaagagaata tatggataaa agagcaaaat
    10861 ggtaatgttc ttggaatgaa gaacaaagaa caaaatgttt ttggtactta tattcatgga
    10921 atctttgatg agggagactt tggagaaaag cttataaata agttaaagaa agaattaaac
    10981 atagaagaaa gtaatgatgt taattataaa gattataaaa tgagtcaata tgataagctt
    11041 tgtgaacttt tagaagagaa tatagacatg gcatatgttg agaatttaat tagatattaa
    11101 ttttaagcag tgtattaagt tttattattt aatacactgt tttttattta aaccattgtt
    11161 gattttagct agaagattag attcaattaa tttttagtaa aaattcagat tttaaatttg
    11221 gtattttaaa tttaaatata acgacatgtt ttaaatttaa ataccttagg tttttaaaaa
    11281 attttatcat tggtaactta ggttacgtga ttattaaagc atatgatata gaataatatt
    11341 tgctaaggcg aaaataaaag ctttaaataa caaaggaaaa gtttttaata aagtggagga
    11401 gtaataatgg aaaataaaat gttttgtttc cagtgtcaag agactgctgg gtgtacagga
    11461 tgtactaagt ttggagtatg tggaaagtca cctgatttag caaggatgca ggatttatta
    11521 atatatacaa ctaaaggatt atctgaagta actactagat taagagaaga aggaaataaa
    11581 atagactcaa aagttaatca tttagtaact ataaacttat ttactacaat aactaatgca
    11641 aactttgatg atgaaatatt ctatgaaagg gtaaaggaaa ctttaagagt aaaggaaagt
    11701 ttattaaata ctttaaagaa taaaaagaat ttatctgaag ctgcactttg gaatgcttct
    11761 aataaaaatg aaatagacga aaaagtaagc ttattacaaa aaataaaaaa tgctttttca
    11821 aagaaagaag agatttcaat agattcaata ttagatgaaa aatcaactaa agtaggagtt
    11881 ttagctacta aggatgaaga tataagaagt ttaagagaac ttataatata tggtttaaag
    11941 ggtatgtcag catatatgaa gcatgctaat gctttagggt atgataaaga agaaataaat
    12001 gcgtttatgc aaagcacatt agctaagact ttagatgata gtttaacaat agatgaatta
    12061 atagctttaa ctttagaaac tggtaaggtt ggagtagatg gaatggcatt attagatagt
    12121 gcaaatacag gaacttatgg acatcctgaa gttactaagg ttaatatagg agttagaaaa
    12181 aatccaggta tattagtatc aggacatgat ttaaaagatt tagaaatgtt attaaaacag
    12241 acagagggaa caggagtaga tgtatataca cattcagaaa tgttaccagc acattattac
    12301 ccagcattta agaaatattc tcattttgca ggaaattacg gaaatgcttg gtggaaacaa
    12361 aaagaagaat ttgaaagctt taatggacca atattaatga ctacaaactg tatagtacct
    12421 ccaaaagata gctacaaaga tagattatat acaactggag ctgcaggttt tgctggatgt
    12481 actcatataa aaggaaactc agaatcagat aaagatttct cagttataat tgagcatgct
    12541 aaaaagtgta aggctcctaa acaaatagaa acaggagaaa ttataggtgg atttgctcac
    12601 aatcaagttc ttgctttagc agataaagta gtggaagctg ttaagtctgg tgcaataaaa
    12661 agattctttg ttatggctgg ttgtgatgga agagctaaat caagaaatta ctatactgat
    12721 tttgcaaaag cattaccaaa agatactgta atattaacag ctggatgtgc aaaatataaa
    12781 tataataagt tacctttagg agatataaat ggaattccaa gagtattaga tgcaggacaa
    12841 tgtaatgatt catattcact tgcattaata gcattaaaac ttaaagaagt atttgaatta
    12901 gaagatataa atgatttacc aatatcattt aatatagctt ggtatgagca aaaagcagta
    12961 atagtattac tatcactatt atatttaggg gttaaaaata tacacttagg accaacatta
    13021 ccagcattct tatcaccaaa tgttgctaaa gttttagttg agaattttgg tataggtgga
    13081 ataactaatg ttgaagatga tataaaaatg tttttaaata actaatattt atataattaa
    13141 aataaaaaat gcaaggataa ttatccttgt attttttgtg ttttgtagta aatagagtat
    13201 tatctcaata tacttacaat attgaaatat aagatgatta cgtaattatt tattattata
    13261 atggaaaaaa gataagcaaa aatgacttgt tacttatagt agctttgaaa gaaaaatgtg
    13321 aattttcttt ggttacagag gattttaatt taagttatat tgctaaagag ttattaggag
    13381 aaaatagagt tctaaatata aaagatattc taatgtaaat tataagctta tatttgatat
    13441 atacttaaat cttatgtatt atattatata gcaagttaag tttaatatta ctttaacttt
    13501 aatctatgat agatatttat aatgggtcaa ttttaaaaga gaaagttcac ttgaagaccc
    13561 caataaaaat tagagataag agtttatttt ataaaaatgt taagtttaac tttaataata
    13621 gattgtaagg tgagttttaa tggatgattt aataaaagca attgataaga ttacagaaga
    13681 ggaaatcata aaactagtaa ttagtaataa attaaataag gatactgaat ataaaaaaat
    13741 agtatttcag cttaaagaaa aaaataaaaa ggatttttat caaatagaga aatatactga
    13801 aaaacaagtt tttcatgaaa atatagaaaa aaaagaactt aaagagaaga tagaagaatt
    13861 atttgtttct aaatacaagc aattaaatgc tttttctaag gaaagttctt tcgatcttaa
    13921 ggtttctaaa aaagggaagg tgtttttagg aattaagaaa aataatgagg ctttaaaaat
    13981 tttaaagaag gaacataata gaaagaaaaa ctacatatta gaagagggaa tgattataga
    14041 gcctcttata gacttagggg tgtttactaa agaaggaaag gtcataaact ctaaatatga
    14101 taaatacaag caaataaata gatttataga aataatagat gatgaaataa aaaagataga
    14161 tcaaaaagag cttacaatat tagattttgg atgtggtaaa tcatatttaa catttatatt
    14221 atattattat tttgttgaaa ttaaaaaaat aaaagttaat atgataggat tagatttaaa
    14281 ggctgatgtt ataaagaaat gtaatgaaat agctaaaaaa tataattatg aaaatttaca
    14341 ttttgagctt ggagatataa atggatttaa atataataat aaagtagata tggtaataac
    14401 attacatgct tgtgatacag caacagatta tgctttatat aatgcaataa agtggaatag
    14461 taaaatgata ttctcagttc catgttgtca acatgaattt aatagtcaaa tagaaagtga
    14521 aaagttttct atacttacta actatggaat aattaaagaa agaatatcag cacttatgac
    14581 tgattcagtt agagctaatc ttttagagtg tgcaggatat aaaactcagc ttttagagtt
    14641 tatagatata gcacattcac ctaaaaatat attaataaga gcaactaaag gaaatgtttc
    14701 taagggtaag aaagaaaagt ctttaaatga ggttaataat cttatgtctg agtttaattt
    14761 aagcccaaca ttatataagc ttttaa
//
LOCUS       DAEVGI010000052        14621 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEVGI010000052 DAEVGI010000000
VERSION     DAEVGI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.052"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            250..933
                     /locus_tag="K8M77_001335"
     CDS             250..933
                     /locus_tag="K8M77_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7085836.1"
                     /translation="MKILLVEDEESIRGFLRINFQRENFQVIECESGEEGVRKALIEK
                     PDIAILDVMLPGMNGFEVCQKLRESFPRMGIIMLTAKGEDMDKIMGLQFGADDYVLKP
                     FNPLEITLRVKALIRRLEGINEEDNNDCIEVDNFKLDLYSQSLYKENVEIDVTPKEFL
                     LMKIFMQNPGKAFTRDELLNLVWGIDFFGDPKIVDVNIRRLRSKIEENSSKPKYIETV
                     WGTGYRWKR"
     gene            1114..2529
                     /locus_tag="K8M77_001336"
     CDS             1114..2529
                     /locus_tag="K8M77_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7085837.1"
                     /translation="MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILL
                     SQINISVDFYKRYLSNVSLEENVYEDVDIFWKQTDAQVQIYNLKGHLIMDSIGLEPKE
                     YKTPVDVKRALEGDISKWIGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDN
                     FILNFFLVFLVIGITVLAIGIILSYFLANSIVNPITELIKVSEQMAKGNLKVRNRIVT
                     NDEIEKLADSLNIMAEEIENREMLKNEFISSISHELRTPLTSIKGWAITLNNDFTDRE
                     TLKMGFDIIEKEADRLSNMVEELLDFSKFVSGKIKLKYEEINLKEFIEYLRLYMNPRA
                     EREHKELILKGITEDFIIVGDKDRLKQVFINIIDNAFKFTHENEKIIIEFLYDEEGIY
                     INIIDTGCGISKEELPRVKEKFYKGKNSKSQNGIGLSICDEIIALHEGNLEIYSELGK
                     GTKVVIYLPKKLIRSVEDDLN"
     gene            2546..4069
                     /locus_tag="K8M77_001337"
     CDS             2546..4069
                     /locus_tag="K8M77_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085838.1"
                     /translation="MKKIFLVFFTIISFFMMGCSQKESFDVSKITPPESDNIKILGKW
                     KVVSKLHDSDENNSNSVSDAFHLEKDDIVNISKDDVVVNGINIVNPNFKLKRMEREIF
                     FKEIDNISIKNEIKDEIKNEYIDVNSIYDSNKTYLSIISINDDEAYLLLTDNLIKLKK
                     ISNEGIKEDSNESNKLDSDKSNKRENKISTEYYQKDVGILLGLKEPANIEDDDFEEAS
                     YKTLWISVINNELQPVMVLDKSLLLPRINGFSNISLSSTLDNGKFENKLKVTSKKKDP
                     NDKTEVKGEKNTQGIYEEITFVGNDYIGLESYSGHDDFRGTFNAYNIIPINTMNSNKS
                     MDILNLFGKEQEENFNKSENNAIQKYDINGRNYYSKNKYSNITLQRKNGNWHLEGILN
                     EKNLMDYPKTFDININPVPILVNYDTLAVPWSQVLSLGKDVRDVVTAPNGKIAIILAK
                     DKLSIYKVIDGRIGERLGEINIKNDEKIVMAEWAIGDYVKCWDETVENVYGAKKTSE"
     gene            complement(4203..6086)
                     /locus_tag="K8M77_001338"
     CDS             complement(4203..6086)
                     /locus_tag="K8M77_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBI7085839.1"
                     /translation="MQEKVKLNNGLSKFKESLNKNALIRLGFYIFTLIAIVLKGALFL
                     GFSLNQNLYTLNFGLGYRQASYFINYYIAFAAIFVSICFLFKNKGKFFSLIIVDLFIT
                     LITVMDIWYFRGFQTVPSLMLLKQTANLDNLGDSIFSMASPYDLLFFVDFMILIIAFI
                     IFRKSFKNCKSNWKGTLIVLLISICYIGYVPFNINVLKRENVKNSYLFSNYDPTNTVE
                     YFSPIGYHIFDIYNVYKNSKPYKMTADDEAKIKEYYDFKNENLPDNKFKGMFKGKNLI
                     VIQVESLEDFVINKKVDGQEITPNINKLLNNSIYLPNIFEQVNEGTSSDSDLMVNTSM
                     LPLRQGSTFFRNPATTYNSLPNILEKDGYSTIAIHSDKGSFWNYAQGLNGIGFDKFVD
                     YYSFDRDENIGLGLSDGSYFRQIEPMIKELKQPFYAFTVTLTSHGPFDLPKEYRQLKL
                     TPELDDNVLGGYFQSVHYTDAKIGMFIESLKKDGLLDNTVIAIEGDHTGPHKYYNSKI
                     ESLPNPEPWWLDNGNHTVPLIIYNPSIKTPVKDDVYGGQIDIMPTLLYLLGVDNNVYQ
                     NTALGRNLLNTKRSYAVLTDKTIKGELTDKEKEIVGNVLDLSDKMIRADYFKDKIPNY
                     NSN"
     gene            6268..6840
                     /gene="yedF"
                     /locus_tag="K8M77_001339"
     CDS             6268..6840
                     /gene="yedF"
                     /locus_tag="K8M77_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBI7085840.1"
                     /translation="MKKIDCLGMACPMPVINTKKYFDSIEEGVAEVLVDNEVAKNNIC
                     KYAEGCGYNFDASEKEGNYIIKIEKNGENKIEKKEDDFVIVVGTDKLGQGNDDLGTIL
                     MKGYLYTLSESDIIPKELIFLNGGVKLTVKGSEVLESLQNLEKRGVKILSCGTCLDFY
                     GIKDDLAIGEISNMYTIVESMNTSNKVIKL"
     gene            6872..7834
                     /locus_tag="K8M77_001340"
     CDS             6872..7834
                     /locus_tag="K8M77_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7085841.1"
                     /translation="MESILCEYDVVVVGGGISGMAAAIRSKENGIGKVLLLEREDVLG
                     GVLNHCIHCGFGKEILGEAVTGSEFVEYLVNKVHKLDIDVKLNTTVLEISKDKEISYV
                     NPDEGMKKMKTRAIVLATGSREKFKGNVSVPLNKFTGIYTSGTAHRFINLYGYLPGKK
                     IVIIGSHDSDMIVARRLIIEGAKVEAIIESKDYLNSSQYIVENIIKAFDIPVKLGYTV
                     NEVLGTERVEGVIISKGGEEESFIKCDSLLISVPWIPESALGKKMGLRISNSSETILV
                     NENFETSESGIFACGNVLHAYELSDLATKEGFKVGDTIKEYLEN"
     gene            complement(7971..8531)
                     /locus_tag="K8M77_001341"
     CDS             complement(7971..8531)
                     /locus_tag="K8M77_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBI7085842.1"
                     /translation="MNIICIGDSLTFGYGVGQENSWVSLLNDEKNKFINKGVNGDTST
                     GILSRIYEILKSSDSNICLIMCGSNDILMNKSISSIIDNISLMTDDCNSLNIKPIILS
                     PPKIYGDLAIKRWASSIDYEKCNSKLENYTRELNLFCEKNNLLFIDLNSNLPFDPLNY
                     TDGLHLSIKGNILVSNLVKMSLKNFL"
     gene            complement(8540..9172)
                     /locus_tag="K8M77_001342"
     CDS             complement(8540..9172)
                     /locus_tag="K8M77_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI7085843.1"
                     /translation="MNSYAKVIVKGSPITKSNFKLSNVHGRAILPSNSGKYHDRYALY
                     EEQIAYEARIQNPNVVLEESLIALLKVYYKSSKRHPDTGNITKSIFDGIEKSGLIIND
                     MQIRRIITEEFYDKENPRFELELFGESSYSFNYEINKREEKVEKLLYSPPPNKKRKTN
                     NTNKEEKNSSINEESSNKVCAICNKAIVGNDFVTANKGTTIICKKCLLKM"
     gene            9314..10333
                     /gene="tsaD"
                     /locus_tag="K8M77_001343"
     CDS             9314..10333
                     /gene="tsaD"
                     /locus_tag="K8M77_001343"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBI7085844.1"
                     /translation="MDKKIILAIESSCDETAAAVVVNGREVLSNIISSQIDIHTKFGG
                     VVPEVASRKHIEAINAVVEEALEVAGVTFDDIDAIAVTYGPGLVGALLVGLQYAKGLA
                     YSLDKPLIGVNHIEGHISANFIDHKDLEPPFVCLVVSGGHTFVVHVEDYGKFEIIGET
                     RDDAAGEAFDKVARAVGLGYPGGPKIDKLAKEGNSDAIKFPKANFHDDTLDFSFSGVK
                     SAVLNYLNKMEMKNEEINKADVVASFQKAVVEVLTDNAIKTCKMRKADKIAIAGGVAS
                     NSALRENLLREGEKRGIKVLFPSPILCTDNAAMIGSAAYFELLKGNISKMSLNAKPNL
                     RLGER"
     gene            10348..11367
                     /locus_tag="K8M77_001344"
     CDS             10348..11367
                     /locus_tag="K8M77_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBI7085845.1"
                     /translation="MRFIPYSNGFHKMNKNIKIKPINVIRNIFIFILALVFIGFAYQM
                     ILNKIDSKKIEPDTKYVRIDSKKNYYNFQGESKPTIIMSSDIGLGLSEWSKVQELIEK
                     EYGYRTFSYDRPGYGFSESVKDDEVKEQAQHLRMILKKSGIGGPYILVGEGYGGLVMC
                     NFAELYPDLVQGVILVDPISEEALSENKDYMKQYSSQKTSRFIQKCGSYFGLTSIMNK
                     FGMLKNTNGLRENLSNENFKVYNILRTKSDFNSGYYSELTNILEQNSSSQKSGLLNGK
                     PLSIIVNDNAFTKEQESLKKLTLDNKVQIINAKNKTDVIPLEKPELFLDSIRFIQDNS
                     LEEQN"
     gene            11484..12812
                     /locus_tag="K8M77_001345"
     CDS             11484..12812
                     /locus_tag="K8M77_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBI7085846.1"
                     /translation="MSDFNKKDEGIDNYFGMEDKENIESNNYTEQTNIDETNKFNIDN
                     EINSKDEVEKEDDKNFSDIKSKNSNDNIKSKKVKKKSGFKRVIALVAGAVIVAILGGA
                     IGASGVYYAFKNSIPVSTLENNSNTQVNPPAFKVEDGALTVPQVVEKVTPAVVGVSTK
                     SLVRDQFFNVKEQEGLGSGFIINEDGYVVTNYHVINGAQEVKVIFSDGKEVNAKVVNY
                     DAERDIAVIKITDNVKMPGIAQLGDSSIVKAGEEVIAIGNPLGKEFSSTVTKGIISSP
                     NRKMKTENGNVLDYIQTDAAINPGNSGGPLINSKGEVIGINTAKKVGEDIEGIGFAIP
                     INEVKTRLGSLSKPILKLGITARTVTPELAKENNIEEGIYVVGVQEFSPAEKSGLKIG
                     DLIIAFGGKRVKTLEELNQIKSQYNDGDSVPIEIIRDGKKVNLNLTLVAN"
     gene            13042..13371
                     /locus_tag="K8M77_001346"
     CDS             13042..13371
                     /locus_tag="K8M77_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI7085847.1"
                     /translation="MKKVVLLCTLGMSTSLLVSKMRKAAKELEIDCSIVAIGEYELKK
                     YEKEADIIMLGPQVRYLFNSLRKKVDSNIPITLINSADYGTMNGNKILNQVLAIMNNK
                     ELSTNNL"
     gene            13534..>14621
                     /gene="eutH"
                     /locus_tag="K8M77_001347"
     CDS             13534..>14621
                     /gene="eutH"
                     /locus_tag="K8M77_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBI7085848.1"
                     /translation="MEKLVLGIIGIFFTISGFDYINNNKLGLGDKFKEGMISMGSIAI
                     SMIGIYSLSPLIGEGIGLLLTPISNFLGIDSSIFPSMFLAVDMGALGIAESLSSNIHM
                     YWISGVIIASTLGATISFSIPLALGIIEEKYLEDLTTGLLYGIMTLPIAPIVAGLFLG
                     VDIKLLLFNIFPLIIFAVLLAVFMNRFKDTTVKFFIKLGKLIQLVSILGLLVLGFLSI
                     IGVKPIGSILPIDEALSVVGKISIFLGGAYPLINFITEKFSKVLSRLGEKINIDEFSI
                     VVFLGTLASNIILFQSFDKMSSKGRMALTAFSVSGAFVIGGQLGFVSLKTPEIINIYI
                     ASKLIAGITAMAVTLILYRKTEENLNNE"
BASE COUNT         5721 a         1456 c         2516 g         4928 t
ORIGIN      
        1 ctttttttgt atttaaagaa aattttattt aatttagtat ttttagtgta agctatttgt
       61 aattaaagaa agtattatat tgtatataat taagaaagaa taaagtagta tcttttttat
      121 tgacaaaaaa ctttagttgt attgatataa ttaaaaagga acaacgcaat attgttacat
      181 aattagtata attagagaat ttaatatatt taaatttaaa aagtatatat aaggagttga
      241 agaatagaga tgaagatttt attagtagag gatgaagaaa gtataagagg ctttttaaga
      301 ataaattttc aaagagaaaa ctttcaagtt atagaatgtg aaagtggaga ggaaggagta
      361 aggaaagctt taatagaaaa gccagatata gcaatacttg atgttatgtt acctggcatg
      421 aatgggtttg aagtatgtca aaagttaaga gaaagttttc ctagaatggg aataataatg
      481 ttaactgcta aaggtgaaga tatggataaa atcatggggc ttcagtttgg tgcagatgat
      541 tatgttttaa aaccatttaa tccactagag ataactttaa gagtaaaagc attaataaga
      601 aggttagaag gaataaatga agaagataat aatgattgta tagaagtaga taattttaaa
      661 ttagatttat attctcaaag tttatataaa gaaaatgtag aaatagatgt aacacctaaa
      721 gagtttttac taatgaaaat atttatgcaa aacccaggaa aggcttttac tagagatgaa
      781 ttattgaatt tagtttgggg aatagacttt tttggagatc caaagatagt tgatgttaat
      841 ataaggagat taagatctaa aatagaagaa aactcttcaa agcctaaata catagaaact
      901 gtatggggaa caggatatag gtggaagaga tagatctgtt tttgtaaaaa tcctaaaata
      961 ttatgagtag gataggattt tgatggaaag gataaatcta tatattacag tgtttatagg
     1021 aattttataa agctatataa aatatagaat taataattaa aacttatatt ttaagttttt
     1081 agtatatata ggtgaaggat aggtgcatat tttatgaaaa gtataaaaac taggctaatg
     1141 aaaaattttt tagttctttt attatcaact attattatag tttcagccat gtttttgatt
     1201 tttatttcta gatattatta tcaaaataca gaggaaatat tattaagtca aattaatata
     1261 tctgtagatt tttataaaag atatttgtca aatgttagtc tagaagaaaa tgtttatgaa
     1321 gatgtagata tattttggaa gcaaacggat gcacaagttc aaatttataa tttaaagggt
     1381 catttaataa tggattccat aggtttagag cctaaagaat ataaaacacc tgtagatgta
     1441 aagagggctt tagagggaga tatatcaaaa tggattggaa cagtgccaga ttatactggt
     1501 aaggtaatgg ctatttcagc accgcttaga aattcttcaa atgaaattgt aggggttatt
     1561 agatatataa gttcacttag gaatgtggat aattttatat taaatttctt tttagtattt
     1621 ttagtaatag gaataacagt tttagctatt ggtataattt taagttattt tttagctaat
     1681 agcatagtaa atccaattac agaattaata aaagtttcag aacaaatggc taagggaaac
     1741 ttgaaagtga gaaataggat tgttactaat gatgaaattg aaaagctagc tgatagcctt
     1801 aatataatgg cagaggaaat tgaaaataga gaaatgttaa aaaatgagtt tatttcttcg
     1861 atttctcatg agcttagaac accattaaca tcaataaaag gttgggcaat aactttaaat
     1921 aatgatttta cagataggga aactttaaaa atgggctttg acataattga aaaagaagct
     1981 gatagattaa gtaatatggt agaggagtta ttagattttt caaagtttgt tagtggaaaa
     2041 ataaaattaa aatatgaaga aataaactta aaagaattta tagagtactt aaggttgtat
     2101 atgaatcctc gtgcagaaag agagcataaa gaacttatat taaaaggtat tacagaagac
     2161 tttataattg taggggataa agatagatta aagcaagtat ttataaatat aatagataat
     2221 gcttttaaat tcactcatga aaatgaaaag ataataatag agtttttata tgatgaagaa
     2281 ggaatatata taaatataat tgatactgga tgcggaataa gtaaggaaga gttgcctaga
     2341 gtaaaagaga aattttataa gggaaaaaat tcaaagtcac aaaatggtat agggttatca
     2401 atatgtgatg aaattatagc tttacatgaa ggcaatttag agatttatag tgaattaggt
     2461 aagggaacga aagtagttat atatttacct aagaaattaa tcaggagtgt tgaagatgat
     2521 ttaaattaat caaaagaggt gttttatgaa gaaaattttt ttagtcttct ttactattat
     2581 tagctttttt atgatgggat gttctcaaaa agaatctttt gatgtatcta aaataacacc
     2641 accagagtca gataacataa agatattagg gaaatggaaa gtagtaagca agttacatga
     2701 tagtgatgaa aataattcaa atagcgtaag tgatgctttt cacttagaga aagatgatat
     2761 tgttaacata agcaaagatg atgtagttgt aaatggaatt aacatagtta acccaaactt
     2821 taagttaaag agaatggaaa gagagatatt ctttaaagaa atagataata tatcaataaa
     2881 aaatgaaata aaggatgaaa taaaaaatga atatatagat gtgaattcta tatatgatag
     2941 taataaaaca tatttaagta ttatttcaat taatgatgat gaagcatatt tattattaac
     3001 tgataatctt attaagttaa aaaaaatttc taatgagggt ataaaagaag atagcaatga
     3061 atctaataaa ttagatagtg ataaaagtaa taaaagagaa aataaaataa gtacagagta
     3121 ctatcaaaaa gatgtaggta ttttacttgg acttaaggag cctgcaaata tagaagatga
     3181 tgattttgaa gaagcttctt ataaaacttt atggatttct gtaattaata atgaattaca
     3241 accagtaatg gttttagata aatctcttct attaccaaga ataaatggat tctccaatat
     3301 aagtttaagt tcaactttag ataatggtaa atttgaaaat aaacttaagg ttacaagtaa
     3361 aaagaaagat cctaatgata agactgaagt aaaaggagag aaaaatactc aaggaatata
     3421 tgaagaaata acctttgtag gaaatgacta tataggatta gaatcttatt ctggacatga
     3481 tgattttaga ggtactttta atgcctataa tataattcct attaatacta tgaattctaa
     3541 taagagtatg gatatattga atttatttgg aaaggaacaa gaggagaact ttaataaaag
     3601 tgaaaataat gctattcaaa agtatgatat taatgggaga aattattatt ctaaaaataa
     3661 gtattccaat ataactcttc aaaggaaaaa tggtaattgg catttagaag gaatattaaa
     3721 tgaaaaaaat ttaatggatt atcctaaaac ttttgatata aatataaatc cagttcctat
     3781 attagtaaat tatgatactt tagcagttcc gtggagtcag gttttatctc ttggaaagga
     3841 tgttagagat gttgttacag caccaaatgg gaagatagca ataatattag ctaaagataa
     3901 gttatcaata tataaagtta ttgatggtag aattggtgaa agattaggag aaataaatat
     3961 aaaaaatgat gaaaagattg ttatggctga atgggctata ggagattatg tgaagtgttg
     4021 ggatgaaact gttgaaaatg tatatggtgc taagaaaaca agtgaataga atataataat
     4081 ttattattaa agtataaata tgggacagta gatagtctgg tgtaaaaaca aaactatcta
     4141 ctgtttttta ttttaggtct ctagaaaata aaaaagaagc tgatataaaa tcagctatct
     4201 ttttaattag aattataatt aggtatttta tctttaaagt aatcagctct aatcatttta
     4261 tcagatagat ctaatacatt tcctactatt tctttttctt tatctgtaag ttcaccctta
     4321 attgttttat cagttaaaac agcgtaagat ctcttagtgt ttaatagatt tctacctaaa
     4381 gctgtatttt gatatacatt attatctacg cctaatagat ataaaagagt tggcattata
     4441 tctatttgac caccgtaaac atcatctttt acaggtgtct taatgcttgg attatagata
     4501 attaatggaa ctgtatgatt tccattgtct aaccaccaag gttcaggatt aggtagtgat
     4561 tctatcttac tgttatagta tttatgagga ccagtatggt caccttctat tgcaataaca
     4621 gtgttatcta aaagaccatc tttttttagt gattctatga acattcctat tttagcatct
     4681 gtatagtgaa cactttggaa gtatcctcct aaaacattgt catcaagttc aggagtaagt
     4741 tttaattgac ggtattcctt tggtaaatca aatggtccgt ggcttgttaa agtaactgta
     4801 aatgcataga atggttgttt taattcttta atcattggtt caatttgtct aaagtagctt
     4861 ccgtcactta atccaagacc tatattttca tcacgatcaa atgaatagta atctacaaat
     4921 ttatcaaaac ctataccatt taaaccttga gcatagttcc agaaagatcc tttatctgaa
     4981 tggatagcaa tagtactata gccatccttt tctaatatat taggtaatga gttataagtt
     5041 gtagctggat ttctaaagaa agtacttcct tgtcttaatg gtaacataga agtattaacc
     5101 attaagtcag aatcagagct tgtaccttcg ttaacttgtt caaatatatt aggtaagtaa
     5161 attgaattat ttaataattt atttatgttt ggagttattt cttgtccatc tactttttta
     5221 tttataacaa agtcttcaag ggactcaact tgtattacta ttaaattttt tcctttgaac
     5281 attcccttaa atttattatc aggaagattc tcatttttga aatcataata ttcttttatt
     5341 tttgcttcat cgtcagctgt cattttataa ggtttagaat tcttataaac attatatata
     5401 tcaaaaatat gataaccaat tggtgagaag tattctactg tgttagttgg gtcataatta
     5461 ctaaataagt atgaattttt aacattttct ctttttaata cattaatgtt aaaaggaaca
     5521 tatcctatat aacaaattga aataagtaaa acaattaagg tacctttcca attggattta
     5581 cagtttttaa agctttttct aaaaattata aaagctatta ttaaaatcat aaagtctacg
     5641 aagaatagta agtcatatgg actagccatt gaaaaaatac tatctccaag attatctaag
     5701 ttagcagttt gttttaatag cattagtgat ggaactgttt ggaatcctct gaaataccaa
     5761 atatccatta ctgtaattaa agttataaat aaatctacaa ttattaatga gaaaaacttt
     5821 cctttgtttt taaataaaaa acatatactt acaaatattg ctgcaaatgc tatgtaataa
     5881 ttaataaaat aagaagcttg tctatatcct aaaccaaaat taagtgtgta aagattttgg
     5941 tttaaagaga aacctaaaaa aagtgctcct tttaaaacta tggctataag agtaaatatg
     6001 taaaaaccta gtcttattaa agcattttta ttaagagact ccttaaattt agaaagcccg
     6061 ttatttaatt taactttttc ttgcattgaa acaccccttc ttttattatt caaagctgtt
     6121 aatattatat cataaaataa tatgttcata gtatgaagga attattacaa aaaagtttaa
     6181 aaacaacttt tttagaataa ctttgatttt ctcaatttaa aaaaatgtga tatctttata
     6241 atatagatta ttttaagagg tgaggtcatg aaaaaaatag attgtttagg tatggcttgt
     6301 ccaatgccag ttataaatac aaaaaaatat tttgattcaa tagaagaagg ggttgcagaa
     6361 gtattagttg ataatgaagt agcaaaaaat aatatctgta agtatgcaga aggatgtgga
     6421 tataattttg atgcttcaga aaaagaggga aattatataa ttaaaataga aaaaaatggt
     6481 gagaataaaa tagaaaagaa ggaagatgac tttgtaatag tagtaggtac tgataagtta
     6541 ggtcaaggca atgatgattt aggtactata cttatgaaag gatacctata taccttatca
     6601 gaaagtgata tcatacctaa agaacttata tttttaaatg gtggagttaa gcttactgtt
     6661 aaaggatcag aagttttaga atcattacaa aacttagaaa agcgtggagt taagatacta
     6721 agttgtggaa cttgcttaga tttttatgga ataaaagatg atttagctat aggagaaata
     6781 agcaatatgt acactatagt tgaatctatg aatacttcta ataaggtaat aaagttataa
     6841 tacttttgag aggagaaaat atttttagag tatggagagt attttatgtg agtatgatgt
     6901 tgtagttgta ggtggcggaa taagtggaat ggcagcagct ataagatcaa aagaaaatgg
     6961 cataggaaaa gttctgcttc ttgaaagaga agatgtttta ggtggtgttt taaatcactg
     7021 catacattgc ggatttggaa aagaaatctt aggagaagct gttactggaa gtgaatttgt
     7081 agagtactta gttaataaag ttcataagct agacatagat gttaaattaa atactactgt
     7141 attagagata agtaaagata aagagataag ttatgttaat ccagatgaag gtatgaaaaa
     7201 gatgaaaact agagcaatag ttttagctac tggaagtaga gaaaagttta agggtaatgt
     7261 aagtgttcct ttaaacaaat ttacaggtat atatacttca ggaactgctc acagatttat
     7321 aaatctttat ggttatcttc cagggaaaaa gatagttata ataggttcac atgatagtga
     7381 tatgattgta gctagaaggc ttattataga gggcgctaaa gtagaagcca taattgaaag
     7441 taaggattat ttaaattctt ctcaatatat tgtggaaaat ataattaaag cttttgatat
     7501 tcctgttaaa cttggatata cagtaaatga agttttaggt actgaaagag tagagggagt
     7561 aattataagc aaaggtggag aagaggaaag ttttataaaa tgtgattcac ttttaatatc
     7621 agttccatgg ataccagaaa gtgctttagg taaaaagatg ggactaagaa tatctaatag
     7681 tagcgaaact attttagtaa atgaaaactt tgaaacatca gaaagtggaa tatttgcttg
     7741 tggaaatgtt ctgcatgcct atgaactatc tgatttggca acaaaagaag gatttaaagt
     7801 tggagatacc attaaagagt atcttgaaaa ttaattttta ataagactaa aattcagatt
     7861 aatatttaag ttaattttga attttagtct tattttatgc tttaaagaag gttataatta
     7921 caaagaaata tttgttttag tgaaattttt tagatttcaa agatatagaa ttaaagaaaa
     7981 ttttttaaac tcatttttac taagttagat actaatatat ttccttttat gcttaagtgt
     8041 aatccatcag tataatttaa aggatcaaaa ggaagattag agtttaagtc tataaacaac
     8101 aaattgtttt tttcacagaa taaatttaat tctctagtat aattctctag cttagaatta
     8161 catttctcat aatcaataga agaagcccac cttttaatag ctaaatcacc atatatttta
     8221 ggaggagata aaattattgg ttttatgtta agtgaattac aatcatcagt cattaagctt
     8281 atgttatcaa taattgaact aatagattta ttcattaaaa tatcatttga gccacacata
     8341 attaaacaga tattagaatc acttgatttt aatatttcat atattctaga taaaatacca
     8401 gttgaagtgt caccattaac ccctttgttt atgaatttgt ttttttcatc atttaaaaga
     8461 gaaacccaag aattttcttg tcctacgcca taaccaaagg taagagagtc tcctatacaa
     8521 attatattca tataatcacc tacattttta gtaaacattt tttacaaata atggttgtcc
     8581 ctttattagc tgtaacaaaa tcatttccta caatagcttt attacaaatg gcacatacct
     8641 tatttgaact ttcttcattt atggaagaat ttttttcttc tttattagta ttattagttt
     8701 ttcttttttt attaggtggt ggtgaataaa gaagtttttc aactttttct tcacgtttat
     8761 ttatttcata attaaaacta taagaacttt caccaaaaag ttctaattca aatctaggat
     8821 tttctttatc ataaaattcc tctgttatta ttcttcttat ttgcatatca tttattatta
     8881 atccactctt ttcaattcca tcgaagatac tttttgttat gttacctgta tcgggatgtc
     8941 tcttagaact tttataataa acttttaaca aagctattaa agattcttct aaaacaacat
     9001 taggattttg aattcttgct tcgtaagcaa tttgttcttc atacaatgca tatctatcat
     9061 gatattttcc cgaattggat ggtaaaattg ctctaccatg tacatttgat agcttgaaat
     9121 ttgatttagt aataggtgag ccttttacta taacttttgc gtatgaattc atagtaaacc
     9181 tccaatcata tgatataata atatagatta tatcataatg taacattatg tatttacatt
     9241 tcttgaaagt atttaacaga taaagtataa tactctatgt ttacatgaaa aattaaaatt
     9301 aagagggata aaaatggata aaaaaattat attagcaata gaaagtagtt gtgacgaaac
     9361 agcggcagct gtagtagtca atggtagaga agttttatca aatataatat cttctcagat
     9421 agatatacat acaaaatttg gaggagtagt tccagaggtt gcatcaagaa aacacataga
     9481 agctataaat gcagtggtag aggaagcctt agaagttgct ggagtaacat ttgatgacat
     9541 agatgcaata gcagttacat atggtccagg tttagttgga gcacttttag taggacttca
     9601 atatgctaaa ggattagcat actctttaga taaaccatta ataggagtta atcatataga
     9661 agggcatata agtgctaact ttatagatca taaggactta gagccacctt ttgtttgctt
     9721 agttgtttca ggaggacata cttttgtagt ccatgttgaa gactatggaa agtttgaaat
     9781 aataggcgaa acaagagatg atgcagcagg agaagctttt gataaggtag caagagccgt
     9841 aggattagga tatccaggag gtcctaaaat agataaatta gctaaggaag gaaatagtga
     9901 tgctataaaa ttcccaaaag ctaatttcca tgatgatacc ttagattttt catttagtgg
     9961 agttaaatca gctgtcttaa attatctaaa taagatggaa atgaaaaatg aagaaataaa
    10021 taaagctgat gttgtagcta gtttccaaaa ggccgtagtt gaagtgttaa ctgataatgc
    10081 aataaaaact tgtaaaatga gaaaggcaga taaaatagcc attgcaggtg gagttgcttc
    10141 taatagtgct ttaagagaaa accttcttag agaaggagaa aagagaggaa taaaggtttt
    10201 attcccatca ccaatacttt gtacagataa tgctgccatg ataggaagtg ctgcatattt
    10261 tgaattatta aagggaaata tatctaaaat gagtcttaac gcaaaaccta atttaagatt
    10321 aggagaaaga taggggctga attatcaatg aggtttattc cgtactcaaa tggatttcat
    10381 aaaatgaaca agaatataaa aataaagcct ataaacgtaa ttaggaatat ttttatattt
    10441 atattagctc tagtatttat aggctttgct tatcaaatga tattaaataa aattgatagt
    10501 aaaaaaatag aacctgatac caagtatgta agaattgata gcaagaaaaa ttattataat
    10561 tttcaaggag aaagcaaacc aactataata atgagttctg atataggctt agggttaagt
    10621 gaatggagta aggttcaaga gcttatagaa aaggaatatg gttatagaac tttttcttat
    10681 gatagacctg gatatggttt ttcagaatca gtaaaagatg atgaagttaa agaacaagct
    10741 cagcatctta gaatgattct gaaaaaatca gggattggtg gaccatatat acttgttgga
    10801 gaaggatatg gtggattagt aatgtgtaac tttgcagaac tttatcctga tttagttcaa
    10861 ggagttattc ttgtagatcc aataagtgaa gaagctttaa gtgaaaataa agattatatg
    10921 aaacagtatt caagtcaaaa aactagtaga tttatacaaa agtgtggttc atattttgga
    10981 ttaacatcaa taatgaataa atttggtatg ttgaaaaata caaatggctt aagagaaaat
    11041 ttaagtaatg aaaattttaa ggtatataat attttaagaa caaaaagtga ttttaatagt
    11101 ggatattata gtgagcttac aaatatttta gagcaaaata gtagttcaca aaaatctggt
    11161 ttattaaatg gtaaaccttt gagcataata gttaatgata atgcttttac taaggagcaa
    11221 gagagtttaa agaaactcac tttagacaat aaagttcaaa taataaatgc taagaataag
    11281 acagatgtta tacctttaga aaagccagaa ttatttttag atagcataag atttattcaa
    11341 gataatagct tggaagagca gaattagcct aagaatatag acatatccta tgacatagaa
    11401 atgacataaa catgacatat aaatgacaca aaaaatgggt attatagaca tgtaaaatat
    11461 aaatatttag gagggtatga atgatgagtg atttcaataa aaaagatgaa ggtatagata
    11521 actactttgg tatggaagac aaagaaaaca tagaatcaaa caattatact gagcaaacta
    11581 atatagatga aactaataaa tttaatatag ataatgaaat taattcaaaa gatgaagttg
    11641 aaaaagagga tgataaaaac ttctctgaca taaaatcaaa aaattctaat gataatatta
    11701 aaagtaaaaa agtaaagaaa aagagtgggt ttaaaagggt aatagctcta gtagctggtg
    11761 ctgtcatagt tgctatacta ggaggagcta taggagctag tggagtttat tatgctttta
    11821 aaaatagcat accagtaagt acactagaga ataatagtaa tacccaagtt aatccaccag
    11881 cctttaaagt ggaagatgga gcattaactg ttcctcaagt agttgaaaaa gttacacctg
    11941 ctgttgtagg agtatccaca aagagcttag taagagatca attctttaat gtaaaagaac
    12001 aagaaggatt aggatctgga tttataataa atgaagatgg atatgtagtt acaaactacc
    12061 atgttataaa tggagctcaa gaagttaaag taatattctc tgatggaaaa gaagtaaatg
    12121 ctaaggttgt aaattatgat gctgaaagag atattgcagt aataaaaata acagacaatg
    12181 ttaaaatgcc tggaatagca caattaggag attcatctat agttaaagct ggtgaagaag
    12241 taattgctat aggaaatccc ctaggaaaag aatttagctc aacagtaact aagggtataa
    12301 taagttctcc aaatagaaaa atgaagactg aaaatggaaa tgtattagat tatatacaaa
    12361 cagatgcagc tatcaaccca ggtaatagtg ggggtccatt aataaactct aagggagaag
    12421 ttattggaat aaatacggct aaaaaagttg gtgaagatat tgaaggtatc ggatttgcaa
    12481 ttcctataaa tgaagttaaa actagattag gttctttatc aaaaccaata ttaaaacttg
    12541 gtattacggc tagaactgtc actccagaat tagcaaaaga aaataatata gaagaaggaa
    12601 tttatgttgt aggtgtacaa gaatttagtc cagcagaaaa atcaggatta aaaataggtg
    12661 atttaataat tgcttttggt ggaaaaagag taaaaacttt agaagaatta aatcagatta
    12721 aaagtcaata taatgatgga gattcagtac cgattgaaat aattcgagat ggtaaaaaag
    12781 taaacttaaa tttaacatta gttgctaatt agtttaatta gagatgtata tcaaataatt
    12841 ttattatttt gttatacatc ttttttattg aaaataaaat gtaaaatttt aaataaaaat
    12901 caaatatatt gaaaaattcg ttgttaatta atatatttga ttatagaata gtagtaatag
    12961 tcattttaaa tatatatttt atctaaaata aaaaaataca tggtgtaagc aaagaatggt
    13021 attagtttag gaggattata tatgaaaaaa gtagtattat tatgtacatt aggaatgtct
    13081 acatctttat tagtttctaa aatgagaaaa gctgcaaagg aattagaaat tgattgttct
    13141 atagttgcaa taggagaata tgagcttaaa aagtatgaaa aagaggcaga tataataatg
    13201 ttaggacctc aagtaaggta tttatttaat agtttaagaa aaaaagtaga tagtaatata
    13261 ccaataactc ttataaatag tgcagattat ggaaccatga atgggaataa gattttaaat
    13321 caagtcttag caataatgaa taataaggaa ttatccacaa ataatttgta agaaatttta
    13381 atatgtgatc gtataagaag tctttttaaa aagagtctta tacgattttt tatttatata
    13441 tttaataaat tgaaaattta aatatatata tattgaattg aataaattag tcaaatatac
    13501 taaaaaatag ttaggtttta aggaggggag tatttggaga aattagtgct tggtataata
    13561 ggtattttct ttacaattag tggatttgat tacataaata ataataaatt gggattagga
    13621 gataaattta aagagggaat gatatctatg ggatcaatag ctatatccat gatagggata
    13681 tattctcttt cacctttaat aggagaaggc ataggattat tattaactcc aataagcaat
    13741 tttctgggaa ttgattcatc catatttcca tcaatgtttt tagctgtaga tatgggagcc
    13801 ttaggaattg cagaaagttt atcatcaaat attcacatgt attggatttc tggagtaata
    13861 atagcttcga ctttaggggc gaccataagc ttttctattc ccttagcttt aggaatcata
    13921 gaggagaagt atcttgaaga cctaacaaca ggtttattat atggaataat gactttacct
    13981 atagcgccaa tagttgcagg tttattttta ggagttgata ttaaattatt actatttaat
    14041 atatttccat taataatatt tgctgtatta ttagcagttt ttatgaatag gtttaaagat
    14101 actacagtaa aattctttat taaattaggt aagcttatac aacttgttag tatattaggg
    14161 cttttagttt taggattttt atctattata ggagtaaagc ctataggaag tattttacct
    14221 atagatgagg ctttaagtgt ggttggtaaa atatctatat tcttaggagg agcatatcct
    14281 ttaattaatt ttataacaga aaaattttca aaagtcttaa gtagactagg agaaaagata
    14341 aatatagatg agttttctat tgtagttttt ttaggaactc ttgcctcaaa tataatatta
    14401 ttccaaagct ttgataagat gagctctaag ggaagaatgg ctttaactgc ttttagtgta
    14461 agtggagcct ttgtaattgg aggacagcta ggatttgtat ctcttaagac acctgagatt
    14521 ataaatattt atatagcatc aaaattaata gctggtataa ctgccatggc tgtaacatta
    14581 atattatata gaaaaacaga ggaaaacttg aataatgaaa g
//
LOCUS       DAEVGI010000053        14539 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEVGI010000053 DAEVGI010000000
VERSION     DAEVGI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14539
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.053"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(100..1755)
                     /locus_tag="K8M77_001348"
     CDS             complement(100..1755)
                     /locus_tag="K8M77_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBI7085849.1"
                     /translation="MKSFRVNVNGESLLFNEGEKYLAILKRDYINEKIPITLAKQRNK
                     YYEFTDLINNDGDVELINISDPHGKIAYSRTLQFVFIKATLDLFKEARINIEHSISKG
                     TFGEIHKEKALNEEDILKIKRKMQEIINKNIIIEKIKMPKKEAIDIFKSYKMQDKVDL
                     LHWVQEDYVNLYKLEDRYDYFYGDMAYSTGILKYFDLLYYNPGFILMVPSHKEPTVVP
                     VFKEQKKLFNVFRENETWLNILGTPDVGSLDYKLEGEESKDIIRVSEALHEKKIAQIA
                     DMISERKEVKIVLVAGPSSSGKTTFSKRLSIQLRVNGYIPIAISLDDYFINRIHTPLD
                     KEGKPDFESINALDLELFNKNLYDILEGEEVEIPSYNFKIGEREWLGNKIKLPDNGVL
                     IIEGIHGLNPKLTEKIPSKNKFKVYISALTQLNLDNHNRIPTTDVRKIRRIVRDSLSR
                     GYGAEQTLEMWNSIKRGEEKNIFVYQEEADVMFNSTLVYELAALKEYALRELEKVDKN
                     SSVYYEAKRIINTLKFFVEVKPERVPENSILREFIGGSCFYKY"
     gene            1883..2581
                     /locus_tag="K8M77_001349"
     CDS             1883..2581
                     /locus_tag="K8M77_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBI7085850.1"
                     /translation="MLITFIRTAILYFIVIVSIRLMGKRQVGELQPYELVITLMLSDL
                     ASLPMQDTRLPLLLGIVPIITLLFIKILISEIQQHSCLFEKLLDGTPSIIISNGEINL
                     EMMKKQKLTMNDILEELRSAGYLDISDIQYAIIETNGTISIIPKSACDNVKRKDLKIK
                     ESESKIPIVLLENGNLNKKVLKQINKDENWLDEKLKSLNYPPRDKLFLVMIDSNGKLF
                     IQRKNQKDKEDIIL"
     gene            2578..3012
                     /locus_tag="K8M77_001350"
     CDS             2578..3012
                     /locus_tag="K8M77_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBI7085851.1"
                     /translation="MKNTLISSVIFFSLLASVLFFNNKLINVCDKVNDYSNKIENSLS
                     KEDWTESYKESLKLSNFLEENYVSLSLYINHEQLDNLSKEILSLTQYIKSEDVSNSLS
                     SIHIIKSYAKHIKDIQKVNIGNILNSNLKNIFYCGIYEDFSL"
     gene            3107..3973
                     /gene="fba"
                     /locus_tag="K8M77_001351"
     CDS             3107..3973
                     /gene="fba"
                     /locus_tag="K8M77_001351"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBI7085852.1"
                     /translation="MALVNAKEMLNKAREGKYAVGQFNINNLEWTKAILLTAQENNSP
                     VILGVSEGAAKYMCGFKTIVGMVNGMLEELKITVPVALHLDHGSYQGAIDAMDAGFSS
                     VMFDGSHYSIEENIVKTKEIINLAAAKNVSVEAEVGSIGGEEDGVVGAGEIADPAECK
                     QIAELGVTMLAAGIGNIHGKYPANWAGLNFEALANIKAATGDMPLVLHGGTGIPSDMI
                     AEAISLGVSKINVNTECQLSFAEATRKYIEAGKDLEGKGFDPRKLLNPGFEAIKATVK
                     EKMELFGSVNRA"
     gene            complement(4021..5064)
                     /locus_tag="K8M77_001352"
     CDS             complement(4021..5064)
                     /locus_tag="K8M77_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBI7085853.1"
                     /translation="MEYVNDINIIEAVIHILDSNSDEPILNEYKLELTDEIYKFIYKH
                     VEKGLKSEDLKYAIFNPERHIVKEVSQDFLNGVNNDIVEVSKELARQMFYIMKANINI
                     PSCDLLTVSISTDQGPMLGILKMDYIKNFTHKVDFVHDKIGIGIVEQAAGLPASGQKV
                     QKCAFIKPIREDQKVNLMVIDKKKKSSEEDELSLNYFVDNYLGCTVFQNERDMTKTFV
                     KATEEWTRNNIIEDAEKAEGIRSKIKNKLKEEEIINIEELSRELFNEEEPILKSDFTT
                     FVRGHGLENDVEVDKLWIEKKMKRTRLKIDKEIDLYIDDEAYHDDSKFEIVRNGDGSI
                     NMVIKNIKNYIEK"
     gene            5204..5767
                     /locus_tag="K8M77_001353"
     CDS             5204..5767
                     /locus_tag="K8M77_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBI7085854.1"
                     /translation="MKELETRIIDIDVEKIRETLNKLGAKNVKRENQTNDIFDFPDRK
                     LLDKKGYARIRTVEDLINNKTVYFMTTKKMLSQDKFKVMEENETIIEDGAMGKNIFKS
                     LGLDLFETIKKYRESYKYKDSLIEIDINDPSFCPFPYIEIETSSEEKLNEILIDLGYS
                     MEDTTSKTIYEILKDKGISPDSNKKGL"
     gene            complement(5894..7543)
                     /locus_tag="K8M77_001354"
     CDS             complement(5894..7543)
                     /locus_tag="K8M77_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI7085855.1"
                     /translation="MNRNKIAALIFAVSTVCVISAHSENAYATEHKNSNQLEENNQNR
                     SVKKGQVINVSTNLRIRKSPNTSSDVIGYLTNGEIFNIDEKDGSWYKINGNGKVGYIH
                     GDYVKELSGNSNSNNNSVSSSNLDTSLVGKKGTVVNVSTSLRVRQSPSTSSSVVGSLR
                     GGQTFEIKGKSGSWYYINSNGLTGYIHGDYVQVGENSSNNGGQSSGNNSGMDTSLAGK
                     TGKVVNVSTSLRIRQSPSTSSSVVGSLSAGQTFNINGKNGAWYNIDAQGTKGHVHGDY
                     VQVLSGNESSNSGSNNNQSESQNNNLDESYNGKAGKVVNVTTNLRLRSQPSTSSSVLA
                     YLLPNERFTLQGKTVSGWFKVNYNGKIGYLHEDYVKIVSSNESSNGAVNENLGSSQNG
                     STGNGQVNQSKYEKVLSVMKSQIGSPYIYGGVGETLNSSLLNSLRRTFPDHAARGFYD
                     IPSKYLNGNYRAFDCSGLMQWSFRQAGISLGRTTWDQVNNGYEVSPSNAKPGDLLFFS
                     NLGHVGMYIGNGQWIEAPNKGKFVSIAPVPWSKIGRARRVL"
     gene            complement(7709..8077)
                     /locus_tag="K8M77_001355"
     CDS             complement(7709..8077)
                     /locus_tag="K8M77_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HBI7085856.1"
                     /translation="MNKNDLNKIEEGYYGNKDDYYDKQGHCYTSFFISDIIIDNNKKG
                     SVTINNNFDICKEKKDFLKEELLKYFKIKKNENLNDEELDKFIRFFVENLGAQFYIQG
                     VIDSYKYIVEKSDDLLSLER"
     gene            8265..8651
                     /locus_tag="K8M77_001356"
     CDS             8265..8651
                     /locus_tag="K8M77_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085857.1"
                     /translation="MYKAIGINMDKEYKALRKKSKRFVPPSHYRDNYSMNNSKEDDLE
                     DYSLFDSMEDLKDCANSNDEDCKIRSCLLNKEIQANKKVLIASCIGAAAGIIGVTCAM
                     KKMNSKSNKSLLKSAPSLMKFAKTML"
     gene            complement(8778..9263)
                     /gene="ybaK"
                     /locus_tag="K8M77_001357"
     CDS             complement(8778..9263)
                     /gene="ybaK"
                     /locus_tag="K8M77_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBI7085858.1"
                     /translation="MAKDKKLKTNAMRILDSKKVSYEMLSYESEDGKIDGISVAHKIG
                     VDEKNVFKTLVAQGTSKELYVFVIPVAEELDLKNAAKIAGEKKVEMIAVKDIMKYTGY
                     IRGGCSPIGMKKNYKTFIHESAEDLDFIIFSAGKIGHQIKMNPKDLVSVVEGEFAFLI
                     K"
     gene            complement(9669..10034)
                     /locus_tag="K8M77_001358"
                     /pseudo
     CDS             complement(9669..10034)
                     /locus_tag="K8M77_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10512..11072)
                     /locus_tag="K8M77_001359"
     CDS             complement(10512..11072)
                     /locus_tag="K8M77_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7085859.1"
                     /translation="MKGLIVFGEKGSGKDTVAKLINEYSEKSVSFFNIGDLVRDMSCI
                     FLATDKWENKKREFYVDTAIKLKEIDKDFLSYYVLGKILDKFKKKSMKDIDNEQLIIV
                     TGGRTYEDFEFWKKSGFKTLGVKCDEKVRIERLKIRDGYEQNSQDDLEKNTRKIIDLC
                     DFTVDNSGSFKDLTKEVTDFVVENGL"
     gene            complement(11151..12101)
                     /locus_tag="K8M77_001360"
     CDS             complement(11151..12101)
                     /locus_tag="K8M77_001360"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBI7085860.1"
                     /translation="MLNVTLLGTGGGMPMPNRFLSSVVMNFKGRKILLDCGEGTQVSM
                     RVNGTGFKSIDIICISHLHGDHIYGLPGLLSTIGNSGRVEEIYIIGPKGIKEVIDGFL
                     ITLPYLPYKLNILEDVSDLEFMVKKEKMELVELNEKISSDLSIKTLELDHSSPCLGYS
                     FNIRRGRKFNVEKALMNKVPKEVWSKLQRNEEVSLNRVKYYSYMVLGDERKGIKLSYT
                     TDTRPTEDIPGFIKESDLYICEGTYGSEEDMHKAIKNKHMTFKEAADLAKKGQVKKLL
                     LTHFSPAINDPKEYIHNAREVFENSHAGSDGEERILNFKE"
     gene            12328..12954
                     /locus_tag="K8M77_001361"
     CDS             12328..12954
                     /locus_tag="K8M77_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7085861.1"
                     /translation="MAKRTKTPNYIKIAIDIANRVLNNDFKEGSKITGRTTLVSIYKV
                     SPETIRRSVALLKDMNVVSVNEKSGIIINSKENAKDFLIKFKAQSDFTTLSNDTYKIL
                     KAKNELDKQLEKNIENIIEFATQLRNVGTVIPFESIVESESFAVGKSIGDLNFWQNTK
                     ATIIGVKRKGEMFLSPGPYFKIMADDIIMYVGEDEVLKNVKNYISNNE"
     gene            complement(13006..13758)
                     /locus_tag="K8M77_001362"
     CDS             complement(13006..13758)
                     /locus_tag="K8M77_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085862.1"
                     /translation="MSKQKKEFLMIGIGLIGLSLFLHYVHYLIFQDLHHTLIFLFADI
                     AFIPMEVFFTTLVIDKLLEKREKEHLRDKLSMLIGVFFSELGTDILNTFVYADDNTEI
                     IAKEALVTKEWNKNEFKNLEKLVNEYEYDIDIKKVDLIALEKKLNSQKDLVINLITNP
                     MLIEHEEFSDMLMSILHLREELSSRCSHELEDYEIKHLAKDINVAYKYLTFEWIQYMK
                     QLKANYPQLFLKALITNPFDKRSLKEKECSYL"
     gene            complement(14056..>14539)
                     /locus_tag="K8M77_001363"
     CDS             complement(14056..>14539)
                     /locus_tag="K8M77_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085863.1"
                     /translation="EHELNKVKNIYDNVVKEMNLRGLYNWNESYPNTEIIFKDISNKH
                     LYGIETSGEIVAVATINDYAYDVYNDIAWQVDGPFLSFHRIAVSPSHRGKGYGRKMID
                     FVEEMAKEKKCNSIRISAYHKNENAVNLYKNLGYVHVGEMMSMRDIVEPFLCLEKSIM
                     "
BASE COUNT         5076 a         2390 c         1515 g         5558 t
ORIGIN      
        1 actaatcttt ataaattgta ttaatccctc cttaatttct aatatatttt aagtattaca
       61 gataataaat tccttactat atttttaatt ttaatttaat taatacttat agaagcaact
      121 tccacctata aattctctta aaatcgaatt ttcaggtact ctctctggct taacttccac
      181 aaaaaacttt aatgtattta ttattctttt agcttcatag taaacactgc tatttttatc
      241 tactttttct aattctctta aggcatattc ctttaacgct gctaactcat aaactaaagt
      301 ggagttaaac ataacatctg cttcttcctg ataaacaaat atgttctttt cctctcctct
      361 ttttatagaa ttccacattt ctaaagtttg ctctgctcca tatcctcttg ataaggaatc
      421 tctaactata cgccttattt ttcttacatc tgtagttgga attctattgt gattatctaa
      481 atttaattgt gttaaagcag aaatataaac cttaaactta ttctttgaag gaattttttc
      541 tgtaagtttt ggatttagtc catgtattcc ctctataata agcactccat tatcaggaag
      601 tttaatctta ttgcctagcc attctctttc tcctatttta aaattataag aaggtatttc
      661 tacttcttct ccttctaaaa tatcataaag attcttatta aataattcta aatctaaagc
      721 atttatagat tcaaaatcag gttttccctc tttatctaaa ggagtatgaa ttctatttat
      781 aaaataatca tctaaggata tagctattgg tatataacca ttaactctaa gctgtataga
      841 aagtcttttt gaaaatgttg ttttacctga agaggaaggc cctgctacta aaacaatttt
      901 gacttctttt ctttcactta tcatatctgc aatttgtgct atttttttct catgcaaagc
      961 ctcagaaact cttattatat ctttagattc ttctccctct aatttataat ctaaagaacc
     1021 tacatctgga gttcctaata tatttagcca tgtttcattt tctctaaata cattaaataa
     1081 ctttttttgt tccttaaata caggtacaac tgttggttcc ttatggcttg gtaccataag
     1141 tataaaacct ggattataat ataataaatc aaaatatttt aaaattcctg tagagtatgc
     1201 catatcccca taaaaataat catacctatc ttctaattta tataagttta cataatcttc
     1261 ttgaacccaa tgtaataaat caactttatc ctgcatctta taagatttaa atatatcaat
     1321 agcctctttc ttaggcatct ttattttttc tattataata tttttattta ttatctcttg
     1381 catttttctc tttattttta gtatatcctc ttcatttaag gctttttctt tatgaatttc
     1441 cccaaaagtt cccttgctta tagaatgctc tatatttatt ctagcctcct taaataaatc
     1501 taaagtagct tttataaata caaactgtaa agttctgcta taagctattt ttccatgtgg
     1561 atcacttata tttataagtt ccacatcccc atcattattt attaaatcag taaactcata
     1621 atacttattc ctctgtttag ctaaagtaat aggtattttt tcatttatat agtccctttt
     1681 taaaattgct aaatattttt ctccctcatt aaagagtaaa ctttccccgt ttacattaac
     1741 cctaaagctt ttcataaaca ccattcctct ctgtttataa ccacttattg ttattataca
     1801 cttaaaactt caaaaaaatt atctattttt gtataaatcc actttaaatt tgttaaccta
     1861 tcttattaga ggaggtattt ttatgctaat aacttttata agaacagcaa tactttattt
     1921 cattgtaatt gtaagtatta gacttatggg taaacgtcaa gttggagaat tgcaacctta
     1981 cgagcttgta ataaccttaa tgctatctga tttagcatcc ttaccaatgc aagatactcg
     2041 tcttccgctc cttttaggca tagtacctat aattacactt ttatttataa aaatattaat
     2101 atctgaaatc caacaacata gctgtttatt tgaaaaactt ctagatggta ctccttccat
     2161 aataataagc aatggagaaa tcaacctaga aatgatgaaa aaacaaaaac taacaatgaa
     2221 cgatatatta gaagaattaa gaagcgctgg ttatttagat ataagcgata ttcaatatgc
     2281 aattatagaa actaatggaa ccatatctat tattcctaaa agcgcttgtg ataatgttaa
     2341 gagaaaagat ttgaaaatta aagaaagtga atcaaaaatt ccaattgttt tattagaaaa
     2401 tggtaattta aataaaaaag ttctaaaaca aattaataaa gatgaaaact ggttagatga
     2461 aaaattaaaa tctcttaatt atccacctag agataaatta tttctagtta tgatagattc
     2521 taatggtaaa ttatttattc aaagaaaaaa tcagaaagat aaggaggaca taatactatg
     2581 aaaaataccc taatttcatc agtaatattt ttttccctcc ttgcttctgt tctatttttt
     2641 aataataaac taataaatgt atgtgacaag gtaaatgatt atagtaataa aatagaaaat
     2701 tccttaagta aagaggattg gacggaatct tataaagagt ctttaaaatt aagtaatttc
     2761 ttagaagaaa attatgtttc tttatctctt tatataaatc atgaacaatt agataattta
     2821 agtaaggaaa tacttagtct gactcaatat ataaagagtg aagatgtttc taacagtttg
     2881 tcttcaatcc atataataaa gagctatgcg aagcatataa aagatattca gaaggttaat
     2941 ataggtaata ttttaaattc caatctaaaa aatatatttt attgtggaat ttatgaagat
     3001 tttagtttat aatgttattg aaaggggcaa gtcccacatg gacttttttt gtccctatat
     3061 aaatctaata aaagttaatg aaagattttg aaaggtggta aacaaaatgg cattagttaa
     3121 cgcaaaagaa atgttaaata aagcaagaga aggaaaatac gctgttggtc aattcaacat
     3181 aaacaactta gaatggacaa aagctatatt attaactgct caagaaaata actcaccagt
     3241 tatattagga gtatcagaag gtgctgctaa atacatgtgt ggattcaaaa caatagttgg
     3301 aatggttaac ggaatgttag aagaattaaa aataactgtt cctgtagcat tacacttaga
     3361 tcacggtagc taccaaggag ctatagatgc tatggatgct ggattctcat cagtaatgtt
     3421 cgatggatca cactactcaa tcgaagaaaa catagttaaa actaaagaaa taatcaactt
     3481 agctgctgct aaaaacgtat cagttgaagc tgaagttgga tcaatcggtg gagaagaaga
     3541 cggtgttgtt ggagctggtg aaatcgctga tcctgctgaa tgtaaacaaa tcgctgaatt
     3601 aggagttact atgttagctg ctggtatagg aaacattcac ggaaaatacc ctgcaaactg
     3661 ggctggatta aacttcgaag ctttagctaa cattaaagct gctactggag atatgccttt
     3721 agtattacac ggtggtactg gaatcccttc agatatgatc gcagaagcta tatcattagg
     3781 agtatcaaaa ataaatgtta atactgagtg tcaattatca tttgctgaag ctactcgtaa
     3841 atatatagaa gctggaaaag acttagaagg aaaaggattt gacccaagaa aattattaaa
     3901 tcctggattc gaagctataa aagctacagt taaagaaaaa atggaattat tcggttcagt
     3961 aaacagagct taatagtttt aaataataaa agagagatta gattttctaa tctctttttt
     4021 ttatttctct atataattct taatattttt tataaccata tttatgcttc catcaccatt
     4081 tctcacaatt tcaaatttag agtcatcatg ataagcttca tcatcaatat ataaatctat
     4141 ttccttatct attttaagtc tagttctttt catctttttt tcaatccaaa gtttatctac
     4201 ttcaacatca ttctctaagc catgacctct tacaaaggtt gtaaaatcac tctttagtat
     4261 aggctcttct tcattaaata actcacggga taattcttct atatttataa tttcctcttc
     4321 tttaagctta ttctttattt tacttcttat accttcagct ttttctgcat cttcaataat
     4381 attatttcta gtccactctt ctgttgcctt tacaaaggtt tttgtcatat ctctttcatt
     4441 ttgaaaaact gtgcatccta aatagttatc tacaaagtaa tttaaagata actcatcctc
     4501 ttctgaagat ttctttttct tatctattac cataagatta accttttgat cttctcttat
     4561 aggttttata aatgcgcact tttgaacttt ttgccctgat gctggaagtc ctgctgcctg
     4621 ttctactatt cctattccta ttttatcatg gacaaaatca accttatgag taaagttctt
     4681 tatataatcc atttttaaaa tccctagcat aggtccttga tctgtagata ttgatactgt
     4741 aagcaaatca caacttggaa tatttatatt tgccttcata atataaaaca tctgtcttgc
     4801 tagttcctta gaaacttcca ctatatcatt attaactcca tttaaaaaat cttgagaaac
     4861 ttccttaact atatgtcttt ctggattaaa aatagcatac tttaaatctt cactttttaa
     4921 ccccttttct acatgtttat atataaactt ataaatttca tctgttaact ctaatttata
     4981 ctcatttaat ataggttcat cactgttact gtctaatata tgtataacag cttctattat
     5041 atttatatca tttacgtact ccatctttat cctcccactt ttcttaaatt tgacacatct
     5101 tatattttat caaagtataa aggaaaaatc accatactat tcttttctcc ctttaacaat
     5161 ttgtttataa tagtattata aagaatttgg aggtagttta attatgaaag aacttgaaac
     5221 acgtattata gatatagatg ttgaaaagat aagagaaaca ttaaataaac ttggagcgaa
     5281 aaatgtaaag cgtgaaaatc aaacaaacga tatttttgat tttccagata gaaaattatt
     5341 agataaaaaa ggttatgcaa gaataagaac tgttgaagac cttataaata ataaaacagt
     5401 ttattttatg actacaaaaa aaatgcttag ccaagataag tttaaagtaa tggaagaaaa
     5461 tgaaacaata atagaagatg gagctatggg taaaaatatc tttaaatcat taggattaga
     5521 tttatttgaa actataaaga agtatagaga aagctacaaa tataaagatt cattaataga
     5581 aatagatatt aatgatccat cattttgtcc atttccatat atagaaatag aaacttcttc
     5641 tgaagaaaag cttaatgaaa ttttaataga cttaggctat tcaatggaag atacaacatc
     5701 taaaacaatt tatgaaattt taaaagataa aggtatttcc cctgattcta ataaaaaagg
     5761 actttagata atagtcttaa tttataaaaa taattataaa aatacaaata acttttactg
     5821 aaaaaaaaca agttatattt agtactattt taccaaatat aacttgtttt ataataaatt
     5881 tattatgtta accttaaagg actcttcttg ctcttcctat tttactccat ggcacaggtg
     5941 caatagaaac aaatttacct ttgttaggcg cctctatcca ttgaccattt cctatataca
     6001 tacctacatg tcctaaatta ctaaagaata ataagtctcc aggttttgca ttactaggag
     6061 aaacttcata tccattattt acttgatccc aagttgttct tcctaaacta attccagctt
     6121 gtctaaaact ccattgcatt aatcctgaac aatcaaaagc tctataattt ccatttaaat
     6181 actttgatgg tatatcataa aatcctcttg cagcatggtc tggaaaagtt cttcttaagc
     6241 tatttaataa acttgaattt aatgtttctc caacgcctcc ataaatatat ggagaaccta
     6301 tttgagattt cataacactt aaaacttttt catatttact ttgattaact tgtccattac
     6361 ctgtacttcc attttggctt gaacctaagt tttcatttac cgctccattt gaactttcat
     6421 ttgaagatac tattttaaca taatcttcat gtaaataacc aatttttcca ttataattta
     6481 ctttaaacca tcctgaaaca gtttttcctt gtaaagtaaa tctttcatta ggtaataaat
     6541 atgctaaaac agaactacta gtacttggtt gacttctaag tcttaaatta gttgttacat
     6601 ttacaacttt tccagctttt ccattataag attcatctaa attgttattt tggctttcac
     6661 tttggttatt attacttcca ctattagaac tttcgtttcc acttaatact tgtacataat
     6721 ctccatgaac atgtcctttt gttccttgtg catcaatatt ataccaagct ccattcttac
     6781 catttatatt aaaagtttgg cctgcgctta atgatccaac aactgagcta cttgtacttg
     6841 gtgattgtct aattcttaaa gaagtactta catttacaac cttaccagtt tttcccgcta
     6901 acgaagtatc cattcctgag ttatttcctg atgattgtcc accattattt gaactgtttt
     6961 cacctacttg cacataatct ccatgaatat atcctgtaag tccatttgaa tttatataat
     7021 accaacttcc acttttaccc tttatctcaa atgtctgacc acctcttagg ctacctacaa
     7081 ctgagctact tgtgcttggt gattgtctaa ctcttaaaga agtacttacg tttacaacag
     7141 tacccttttt cccaactaat gaagtatcta aatttgaact acttacacta ttattgttac
     7201 tattagaatt tccacttaat tctttaacat aatctccatg aatataacca actttaccat
     7261 taccatttat tttataccat gaaccatctt tttcatctat attaaaaatt tcaccattag
     7321 ttaaatatcc aataacatca ctacttgtat ttggactctt tctaattctt aaatttgtac
     7381 ttacatttat aacttgtcct ttttttactg atctattttg attattctct tctaattgat
     7441 tagaattttt atgttctgtt gcataagcat tttcactatg tgctgaaata acacataccg
     7501 ttgatacagc aaaaatcaaa gctgctatct tattcctatt catacacaat cctccctagt
     7561 agtcatatta agccatgcct aaaataattc ttcaccttct ataatatatt ctttaataaa
     7621 atttatcaac aaaatagtta caaaaaaata tcaaaaataa caaaaaaaga agttatcctt
     7681 atttaaactg gacaacttct atgttctatc atctttctaa acttaacaaa tcatcagatt
     7741 tttcaacaat atatttataa gaatctataa ccccttgtat ataaaattgc gctcctaaat
     7801 tttctacaaa aaatcttata aatttatcta actcttcatc atttaaattt tcattttttt
     7861 taattttaaa atattttaaa agctcttctt tcaaaaaatc ttttttttct ttacaaatat
     7921 cgaaattatt atttattgta acgctacctt tcttattatt atctataata atatcgctta
     7981 taaaaaaact agtgtaacaa tgtccttgct tatcataata atcatcttta ttcccataat
     8041 atccttcttc aattttattt aaatcatttt tattcattca gattcacgcc ttctaaataa
     8101 atttggaata tattccatat aatacatttt attcaccaat catattaaag ttacttaata
     8161 tattatttta atctttaagt ttttatcatt aaggaaatta taaaaagatt ttaatttgtg
     8221 attatataaa ttaattttgt aaataataaa tttgaggtga ttttatgtat aaagcaattg
     8281 gtataaatat ggataaagaa tacaaagcct taagaaaaaa atctaaacgc tttgttcccc
     8341 catctcatta tagagataac tattcaatga ataactctaa ggaagatgac ttagaggatt
     8401 attcactctt tgattctatg gaagatttaa aggattgtgc taactctaat gatgaggatt
     8461 gcaaaataag atcttgtcta ttaaacaaag aaattcaagc aaataaaaaa gttcttattg
     8521 catcttgtat aggagctgca gcaggtatta taggtgtaac ctgtgcaatg aaaaaaatga
     8581 attcaaaatc aaacaaaagt cttttaaaat ctgctccttc tttaatgaaa tttgctaaaa
     8641 ctatgcttta aaaagtaaaa agaagccaca taaaactttg tggcttctct ttcatttaat
     8701 aattaaattt tattttaaaa attctaaact tttaatttct taaaagttct tttaaaatat
     8761 actctaatta tcttttacta ttttattaaa aatgcaaatt ccccttctac aacacttact
     8821 aagtcttttg gattcatttt tatttgatgt cctatcttcc ctgcactaaa tattataaaa
     8881 tctaaatctt ctgcgctctc atgtataaag gttttataat tctttttcat tcctataggg
     8941 gaacatccac cacgaatata tccagtatat ttcattatat ccttaactgc tatcatttca
     9001 actttctttt ctccagctat cttagcagca ttttttaaat ctaattcttc tgcaactggt
     9061 ataacaaaaa cataaagctc tttgcttgtt ccttgagcta ccaatgtttt aaatacattt
     9121 ttctcatcta ctccaatttt atgagctaca gaaattccat ctatctttcc atcttcactt
     9181 tcataactta acatttcata tgaaaccttt ttagaatcta atattctcat agcatttgtc
     9241 ttaagcttct tatcttttgc cataattttc acctcttaaa cttaaatcat ttataattat
     9301 acaataaaat tctttaaaaa ctaagcttaa ctttatatta actcagataa agttaatata
     9361 aattaagata ttttgtgtcc atattttcag tatataatat caaatcttat tgaaccccaa
     9421 ttctagtttt atggtaaaat acactaatta caaactctta aatctagtaa gattataaac
     9481 atcatatata aagaatttag aagctaaaac acaaactcat ctaaccaaag ctaaattaat
     9541 taagctacat tattatctag atttattcta cttatttact tcaaggtggt catttatgtc
     9601 attccttttc tacacatata taataaaaaa atgctttcat aataaattta ttataaaagc
     9661 atttcttttt atttgtttaa atcttcaaat atagctttat cattatttga aaatttgttt
     9721 aaatcatttg attgtataag attcccattt gtataattta atttagtata acctttttcg
     9781 cctatagtat aaacatatgg ttatatttct ttataatatg atatcatatc aataccgtca
     9841 tcatttttag catcatacaa atctaatttt ttattgaaat tattatcaac acatttatag
     9901 atagcaaact gtccatctac accaaaggat tcaatagtat ctctatctcc tgtagaagag
     9961 tatctaggca cttcattatt tccacaaaat agaaataaaa gaacaaagct taaagctata
    10021 aatattaaag agattttttc atttgttcct cctaaaaaat aattttaaaa ataattagct
    10081 attatattaa tatactatta tcttctatat aattataaaa taaagcactt aaaatatata
    10141 agcataataa ttccaaatta aaatcacagt taaaaactat gcttttaatt cagaatactt
    10201 acacttataa tacttttaca catataatgc taatttaaat gctctgtttt tatcgccgcg
    10261 ttaaatccat cttcaaagcc gtctttaata tctttaaatc cgaaaattaa aaataaaact
    10321 attacaatta aaattcctat tacaaatttc tttttcatat attcccccaa caaaccatat
    10381 attaacatta tattattgta aataaaaaag aataagtaag aatcaaccta cctattcttt
    10441 taatattatt cctaaatatt aattttataa atttattaaa acaccttatt tttatttatc
    10501 ttagtagaat tttataatcc attttcaacc acaaaatctg ttacttcctt tgttaagtcc
    10561 ttaaaagatc cgctattatc cactgtaaaa tcacataaat caattatttt tcttgtattc
    10621 ttttcaagat catcttgtga gttttgttca taaccatctc taatttttaa tctttctatt
    10681 cttactttct catcacattt aacccctaat gttttgaaac cgctcttttt ccagaattca
    10741 aagtcctcat atgttcttcc accagttact attataagtt gctcattatc tatatccttc
    10801 atagatttct ttttaaattt atctagtatt tttcctaata cataataact taaaaagtcc
    10861 ttatctattt ccttaagttt tatagccgta tctacataaa attctctttt tttattttcc
    10921 cacttatctg ttgctaaaaa tatacatgac atatctctaa ctaagtctcc tatattaaag
    10981 aaactaacac ttttctctga atactcattt ataagcttag ctacagtatc ttttcctgaa
    11041 cctttttctc caaaaactat taaccccttc atattatcaa tccctttctt agttataaga
    11101 ttacatatta attataacat ttattggaat ttttaaatat cttattttta ttattcttta
    11161 aaatttaaaa ttctttcctc tccatcactt ccagcatggg aattttcaaa tacttctctt
    11221 gcattatgta tatattcctt tggatcatta atagctggac taaaatgagt taataataat
    11281 ttctttacct gacccttttt agctaaatca gctgcctcct taaaagtcat atgtttattt
    11341 tttatggcct tatgcatatc ctcttcactt ccataagtcc cttcacatat gtataaatca
    11401 ctttccttta taaaacctgg tatatcctct gtaggtcttg tatctgttgt gtaacttaat
    11461 tttattccct ttctttcatc tcctaagacc atataactat aatatttaac tctatttaaa
    11521 ctaacttcct catttctttg aagtttagac cacacttcct ttggaacttt attcattaaa
    11581 gctttttcaa cattaaattt tcttcctctc cttatattaa aagagtatcc taaacaagga
    11641 gaagaatggt ctaattctag agtttttata cttaaatccg aacttatttt ttcattcaat
    11701 tcaactaatt ccattttttc ttttttaacc atgaattcta aatcacttac atcttctagt
    11761 atgttaagtt tatatggaag atatggaaga gttattaaaa atccatctat aacctctttt
    11821 attcccttag gtcctattat atatatttct tctacccttc cactattccc aattgtagaa
    11881 agtaatcctg gtaatccata tatatgatct ccatgaagat gacttataca aataatatct
    11941 atacttttaa atccagttcc atttaccctc atagaaacct gcgtgccctc tccacagtct
    12001 aaaagaattt ttctcccctt gaaattcata acaactgatg ataaaaatct atttggcata
    12061 ggcatgcctc cccctgttcc taacaaggtt acatttaaca tgttaatcaa tcctctcttt
    12121 ctataactaa ttatattata tagcaaaata atatttatta taaaaaagta agctgacttc
    12181 ccttaattag ggtattcttc attatttctt atgcaaaaac aatataactt tatatataaa
    12241 aatatccttt ataatttaca aagctattat ctaagtgata aaatcaattt agtgcaatat
    12301 attaatccta tggagcgtga tattataatg gctaaacgta caaaaacgcc aaactacata
    12361 aaaatagcta ttgatatagc taatagagtt ttaaataatg attttaaaga aggaagtaaa
    12421 attacaggtc gtacaaccct agttagcata tataaagttt cacctgaaac cataagacgc
    12481 tcagtggcct tacttaagga tatgaatgta gtatctgtta atgaaaaaag tggaataatc
    12541 ataaatagta aagaaaatgc caaggacttt ttaattaaat ttaaagctca atccgacttt
    12601 acaaccttaa gcaatgatac ttataaaata ttaaaagcta aaaatgaatt agataaacaa
    12661 cttgaaaaaa acatagaaaa tataattgaa tttgctacac aacttagaaa tgtaggaact
    12721 gttataccct ttgaaagcat tgttgaaagt gaaagctttg ctgttggaaa aagcattgga
    12781 gatctaaact tttggcaaaa tacaaaggca actataatag gagttaaacg aaaaggagaa
    12841 atgttccttt ctccaggacc ttatttcaaa ataatggctg atgatataat aatgtatgtt
    12901 ggtgaagatg aggttttaaa aaatgtaaaa aactatatat caaacaatga ataactaaaa
    12961 aagaagtatg atataacctt atgaggatct atcatacttc tttttttata aataactaca
    13021 ttctttttcc tttaatgatc ttttatcaaa aggattggta attaaggctt ttaagaataa
    13081 ttgtggataa ttagctttta attgtttcat gtactgaatc cattcaaagg ttaaatactt
    13141 ataagcaaca tttatatctt ttgctaaatg ttttatttca taatcttcta attcatgaga
    13201 gcatctacta cttagttctt ctcttagatg taatattgac ataagcatgt ctgaaaactc
    13261 ttcatgttct attagcatgg gattagttat taaatttata actaaatcct tttgagaatt
    13321 taatttcttt tctaaagcta ttaagtccac ctttttaata tctatatcat actcatattc
    13381 atttactaat ttttctaaat tcttaaattc atttttgttc cattccttag ttactaaggc
    13441 ctctttagca attatttctg tattatcatc tgcataaaca aatgtattta aaatatctgt
    13501 tcctaattca ctaaaaaata ctcctataag catacttagc ttatccctta aatgctcttt
    13561 ctccctcttt tcaagtaatt tatcaattac taaagtagta aagaatactt ccataggtat
    13621 aaaggctata tctgcaaaaa gaaatattag tgtatgatgt aagtcctgaa atataagata
    13681 atgcacgtaa tgtaagaata gtgataatcc tattaatcca attccaatca taagaaactc
    13741 tttcttttgt ttactcattc ttgtcctcct catttagtct taagtaattc tcaattaata
    13801 atccttggaa ttttatacta ccatattata gtcctacttt aaaaggtttt taacactttt
    13861 ttcaaattta acttacaaaa ataacttgct ataaaagcta tcattaatat cttaaattta
    13921 aattctataa ttcccgaaag acccttctat aacttctata caaatcatat ttaaatttaa
    13981 atacccttta taataaaatt tataaaaaaa ccaattcata gtctatttaa aactataaat
    14041 tggttcatat tataatcaca ttattgattt ttcaagacat aagaaaggtt ctactatatc
    14101 tctcatagac atcatttctc ctacatgtac atatcctaag tttttatata aattaactgc
    14161 attttcattt ttatgataag cactaattct tatggaattg cacttttttt cctttgccat
    14221 ttcttctaca aaatctatca tttttcttcc atatcctttt cccctgtgag atggtgagac
    14281 agctattctg tgaaatgaca aaaatggtcc atcaacttgc catgcaatat cgttataaac
    14341 atcataagca taatcattaa tagtggcaac agctactatt tctccagaag tttctattcc
    14401 ataaagatgt ttattagaga tatccttaaa aattatttca gtattgggat aactttcgtt
    14461 ccaattataa agtcctctta aattcatttc ttttacaaca ttatcataaa tatttttaac
    14521 cttatttaat tcatgttct
//
LOCUS       DAEVGI010000054        14346 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEVGI010000054 DAEVGI010000000
VERSION     DAEVGI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.054"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..267
                     /locus_tag="K8M77_001364"
     CDS             <1..267
                     /locus_tag="K8M77_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBI7085864.1"
                     /translation="IKLYVDNQLIGISIGERFNDITYIVHVEKCLKEFKGSYAFINNE
                     LLKNSFLDLKYVNREEDLGILGLKKSKMSYNPKFFERKYLVKIK"
     gene            complement(295..1026)
                     /locus_tag="K8M77_001365"
     CDS             complement(295..1026)
                     /locus_tag="K8M77_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBI7085865.1"
                     /translation="MELNICHLYPDLLNVYGDVGNILILKHRAKLRGIKVNVINVSMG
                     DKFDKDNYDIVFFGGGQDFEQSIVSDDLINLKKDVLKEYVEEGKVLLAICGGYQLLGN
                     YYTAPTGEKIDGLGILDIYTEGGDTRFIGNTVIHNEEFDETYVGFENHSGRTFIGDLK
                     PLGKCIHGYGNNGESGYEGCIYKNTFCTYFHGSLLSKNPELADRILTLALSNKYDDVS
                     LSPLDDTFEIKAKNVMIDRLNNENN"
     gene            complement(1044..2402)
                     /locus_tag="K8M77_001366"
     CDS             complement(1044..2402)
                     /locus_tag="K8M77_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBI7085866.1"
                     /translation="MVFKIAIKSYLSIILSKMTQFISKKVFKGGTNFPGKVALKIDRN
                     ILKVVSKNYKVILVTGTNGKTTTTSMIYNVLKGNGLDVITNATGANLYPGIVSTFVAN
                     YKFSSNKERYAVIEVDEANLKFITEYITPEIITVTNLFRDQLDRYGEVYTTLNKILEG
                     VVKVPSTTLLLNGDESLLGRLDVKNPKVYYGFEVSPNKNKNIEINADAKFCKFCKEPY
                     SYNFITYNHLGSFYCPNCGFKREDLKYKVEEIVELTPESSKVVINGGLFTITQPGTYN
                     IYNGLCAYSVAKELHINDEVIQKSFSNQSSSFGRQETIKIGDKEARIILVKNPAGYNQ
                     ALDTLLLNSESFAAAFLLNDNYADGKDVSWIWDVDFEKLNSMDLNEIFISGMRAYDMA
                     VRLKIANLPVEKFLIKEDFEGLTEAIKNSNENKVYILATYTAMINYRKYLHSKGYIDK
                     LW"
     gene            2650..3627
                     /gene="manA"
                     /locus_tag="K8M77_001367"
     CDS             2650..3627
                     /gene="manA"
                     /locus_tag="K8M77_001367"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBI7085867.1"
                     /translation="MYPIRFENLYYDKVWGGRDFELFRDNMPEGMIGETWDVACHKNG
                     MSIVENGEYKGLRFDELIDKLGSDLVGTEISKEKFPLLIKLINAKDKLSVQVHPNDEY
                     GMRVEGELGKTEIWYVVEAFEGANLIVGTKDCTKEQFVKAIETGEFDQYLNRVEVKKG
                     DTFFVRSGLVHAICEGVIIAEIQQNSDTTYRVYDYNRGREIHVEKALDCIDFSLKGEK
                     AKGLKVSYDEYDKTYLSLCEYFSLEKYDIHGVCEEESDKERFFIFTCVEGEGIITSEE
                     GELAIKKGDSIFIPASLGKYSLKGNMTLLKSYVPKEGAVEEEILSVVKA"
     gene            3889..4803
                     /locus_tag="K8M77_001368"
     CDS             3889..4803
                     /locus_tag="K8M77_001368"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI7085868.1"
                     /translation="MGIDFLFILKALIIAIVEGLTEFVPVSSTGHMILVGDLIHFNTQ
                     SGGFPEMYEVVIQLGAILAVVVLYWRKISSSVVEFLSYIFSFIGLKTSDDKRKYEKRL
                     AESKTGFRFGINVIIATIPAAILGFLFDDEINKYLFSTKTVAIGFIVGGILLIVIENN
                     FRKRAKRSKIVKDIDKMTYGQSLLVGCFQCLSLWPGMSRSASTIMGGWISGLSTTVAT
                     EFTFFLGIPVMVGASGLKLFKFDYSQMDGTYLIALILGFIVAFIVSLVVIDKFINYLK
                     KKPMRVFAIYRVFAGIVLAILIFTKVIS"
     gene            5013..6113
                     /locus_tag="K8M77_001369"
     CDS             5013..6113
                     /locus_tag="K8M77_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI7085869.1"
                     /translation="MMQPIKKIAILTGGGDCPGLNAVIRAVTRTAILKYGYEVIGYKF
                     GYRGLYNNDFVKLDLDSVSGILHRGGTILHSSNKDNLFDYQVEDENGKIVKKDVSDVG
                     VENLKKEEVDALVVIGGDGTLTSARDFSRKGVNVIGVPKTIDNDLLATDVTFGFNTAT
                     EIATEALDRLHTTAESHHRIMLLEVMGRNAGWIALDSGIAGSADVILLPEIPYDINKI
                     VEKVKEREESGKQFTIIVVAEGAKPKDGEVVVSKIVDDSPDPVRLGGIANKLAMDLEG
                     LIKNHEIRSTVLGHIQRGGNTSTYDRILSTKYGVKAVELINSNLFGNMVALKGNKVSY
                     ESLENVIGNTKNVDPEGELVNTAKSIGISFAD"
     gene            6345..7124
                     /locus_tag="K8M77_001370"
     CDS             6345..7124
                     /locus_tag="K8M77_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBI7085870.1"
                     /translation="MKEVQKYLESRIGSYSFFFEDLKSGYTYGYNENVKMISAGCMKL
                     PIAIALIKEVEEGKIDFLDKVKIESSDKVYGTGIIHEFGERDYTVFELMVAMLIQSDN
                     TAANKIIDLVGMDKINETIKEFGMKNTELNRKTADERNFKSDVENYTTAYDLTLAWKK
                     LSEGSFLSKENSQMLIDILTRQQIKNKLALYIPDNLKFDISSKTGDKKGVENDTALIH
                     TQKGNFACTIMSSGIPNSVYGTVTLAKSGKMTWDAIMNNWH"
     gene            7536..8459
                     /gene="pyrB"
                     /locus_tag="K8M77_001371"
     CDS             7536..8459
                     /gene="pyrB"
                     /locus_tag="K8M77_001371"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBI7085871.1"
                     /translation="MLKNKHLLDPSDFTIEEFDEIFKLAHQIMTNPKEYQNICNGKIL
                     ATLFYEPSTRTRLSFESAMLRLGGQVIGFSEPNSSSVSKGESLRDTIKTLNCYADLIA
                     MRHPLEGAAKVASMYSDIPVINAGDGGHQHPTQTLTDLLTIKEYKGNLEGNTIALCGD
                     LKFGRTVHSLIKALSRYKNNKFILISPIELRIPNYIREQILEKNNIEYKEITSLEEGI
                     KEADILYMTRIQRERFVDQSEYERLKDVYVLDEAKMKGAKEDMMVLHPLPRVNEIAYE
                     VDEDSRAFYFKQAKCGMYVRMALMAKLLGEA"
     gene            8464..8622
                     /locus_tag="K8M77_001372"
     CDS             8464..8622
                     /locus_tag="K8M77_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBI7085872.1"
                     /translation="MKNRELQNHKCKNTKCITQVEKYVPQSFTLIDKKNNTYNCDYCN
                     AENTFQKH"
     gene            8644..9843
                     /locus_tag="K8M77_001373"
     CDS             8644..9843
                     /locus_tag="K8M77_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBI7085873.1"
                     /translation="MNLLIKNVNLIDESNNFFGDIYIEKGVIKELGTELNKECETLDG
                     KGLVLMPAFIDTHAHFRDPGFEYKEDIESGSKAAVRGGYTTVTLMPNTKPVCSSKEIL
                     DYVVNKGKEVDLVDLYQTVSITKNLSGEEINHLREFEGNPNVKAITDDGKGVSDSKIM
                     MEAMKIAKENNWIVMSHAESPEFSKVDMRLAENMMTWRDITLAKFIDCRLHMSHVSTK
                     EAMKYIIEGKNDGVKVTCEITPHHLALNNKISNYRVNPPIREEEDVNFLIKAIKMNYV
                     DCIGTDHAPHSKEDKEKGAPGMIGIEQAFSICYTKLVKENHISLNKLSQLMSGNAAKL
                     LNLNKGKLQPGFLGDLVLIDLNKKRIFKEEDIVSRSKNTPFNGMEFYGDVVLTIKNGK
                     IVYKGEF"
     gene            9853..10716
                     /gene="pyrF"
                     /locus_tag="K8M77_001374"
     CDS             9853..10716
                     /gene="pyrF"
                     /locus_tag="K8M77_001374"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBI7085874.1"
                     /translation="MIADKLFEKVEKNGVVCVGLDTSLDYIPEEFKSKFSNESDMLFA
                     FNKEIIDATLDVSACFKVQIAYYEALGLKGLEAYKNTLSYLREKNALIIADIKRGDIA
                     ATAKMYAKAHFEGDFESDFITLNPYMGMDSIDPYLPYIEKNEKGVFVLVRTSNKGAQD
                     IEYLEAGDGKKVYDVVGEKLNTLGKNYLGKHGYSSIGGVVGCTHQEEAKEMRDKLDTM
                     PFLIPGYGAQGGTAKDVAAYLKNGNGGIVNSSRKILLAYKAMEDSKNFAECARKEAIS
                     MRDSIREAILK"
     gene            10741..11481
                     /locus_tag="K8M77_001375"
     CDS             10741..11481
                     /locus_tag="K8M77_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBI7085875.1"
                     /translation="MAMEYFKGKVKENIELVEGIYSLVVEHEAKINAGQFYMIKTPNT
                     FLGRPISVCEVNGNDVRFVYATVGAGTNEMKKMISGDEIEIIGPLGNGFDINKDYGRV
                     ALVSGGIGTAPMVELAKGLRKNNKDIKMDFYGGFRDDIYLVDEISEYVDEVKISTNTG
                     KHGHKGFVTEILPLQNYDTVLCCGPEIMMKKVVEMCKEAKVNVYISMEKHMACGVGAC
                     LVCTCKTKSGNKRTCKDGPVFNGLEVEF"
     gene            11501..12400
                     /locus_tag="K8M77_001376"
     CDS             11501..12400
                     /locus_tag="K8M77_001376"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBI7085876.1"
                     /translation="MLKTVINGVDFKNPVIAASGTFGFGAEYNNFYDVGELGGISSKG
                     LTLKGREGNEGIRIYETPQGIMNSVGLQNPGIEKFIEVELPKMLELGTNIIANVGGAT
                     IEDYEKAVSLLNKEKIDMIELNISCPNVKSGGMAFGIKSDVAYEVVSKVRKICDKPLM
                     VKLSPNAEDICNMAYRCAEAGADSISLINTLKGMAIDIKNRKPVFNNVYAGLSGPAVK
                     PIALRMVHEVAKTVDIPVIGLGGISTAEDAIEFMMAGAKAIQIGTINFVNPKAGIEII
                     KGLEDFCKKEGLKNINEIVGCIK"
     gene            12551..13129
                     /locus_tag="K8M77_001377"
     CDS             12551..13129
                     /locus_tag="K8M77_001377"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBI7085877.1"
                     /translation="MKNTDAMVIDTLKEVGALLEGHFLLSSGRHSNRYCQCAQLLKYP
                     EKAEKVLKVVADQLKDIDFDLVVGPAMGGVIVAYELGRQLGKPAIFTERENGEMTLRR
                     GFTIEKGQKVVITEDVVTTGKSFKEAAKVIEEQGGEVVAVVCIVDRTPGNVTDFPMYS
                     SIKLDIESFEAENCPLCKEGVPYIKPGSRNIK"
     gene            13288..13761
                     /locus_tag="K8M77_001378"
     CDS             13288..13761
                     /locus_tag="K8M77_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HBI7085878.1"
                     /translation="MSIFLKYKSKLIFFLTFIIVTLTIIAMIFEGGVPINKPSIKITY
                     NNEKVSTINGEFNWFNKGAGGNSTFAEPIENLKEPLYAKGGEKIKIRFSKEPKSVVIH
                     DISVSPYKDYKFLKEESNKEFSFMLPEEKGEYNFQVDGIWDSTHNVSKIFKVYID"
     gene            13810..14232
                     /locus_tag="K8M77_001379"
     CDS             13810..14232
                     /locus_tag="K8M77_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085879.1"
                     /translation="MNLEKVILKKDLIKDIKSISSLYESAGWFDYTEDLGKLEEAFKN
                     SLKIISAWHEEKLIGLIRVVGDGLTIIYIQDIVVLPEYQGNGIGRGLIDFVLDEYKHV
                     RQKILISDDKESSIEFYKNIGFKSIDTYNGVAFVSYIE"
BASE COUNT         5673 a         1632 c         2477 g         4564 t
ORIGIN      
        1 ataaaacttt atgtagacaa tcaattaata ggaatttcca taggagagag atttaatgat
       61 atcacatata tagttcatgt agagaagtgt ttaaaagaat tcaaaggatc ctatgctttt
      121 ataaataatg aattattaaa aaattctttt ttagatctaa agtatgttaa tagagaagag
      181 gatttaggca tattaggatt aaaaaagtca aaaatgtctt ataatccaaa gttttttgaa
      241 agaaaatatt tagttaaaat aaagtgaggt aatttatcct cactttattt ataattaatt
      301 attttcatta tttaatctat ctatcatgac attcttagct ttaatttcaa aggtatcatc
      361 taaaggtgat aatgaaacat catcatattt attactaaga gccaatgtta aaattctatc
      421 tgcaagctct ggattcttag ataaaagtga tccgtggaag tatgtacaaa aagtattttt
      481 ataaatacat ccctcataac cactttcccc attatttcca taaccatgaa tgcattttcc
      541 aagaggtttt aaatctccaa taaatgttct accagagtga ttctcaaagc caacataagt
      601 ttcatcaaat tcttcgttat gaattactgt gttacctatg aatctagtat ccccaccttc
      661 agtatatata tctaaaattc ctaagccatc tattttttct cctgtagggg cagtataata
      721 attacctaaa agttgatatc caccacagat agctaaaaga acttttcctt cttcaacata
      781 ttcttttaaa acatcttttt ttaggtttat taagtcatca gatactattg attgttcaaa
      841 gtcttgacct ccaccaaaga atactatatc gtaattatct ttatcaaatt tatcacccat
      901 agaaacattt attacattta ctttgattcc acgcaatttt gctctatgct ttaatattaa
      961 aatatttcct acatctccat aaacattaag tagatcagga tataagtgac atatatttaa
     1021 ttccatatga atctctcctt taactaccaa agcttatcaa tataaccttt ggaatgtaag
     1081 tattttctat agttaatcat agcagtataa gttgcaagga tataaacctt gttttcatta
     1141 gaatttttga ttgcctctgt taagccttca aaatcttcct ttattaaaaa cttttcaact
     1201 ggtagattag caattttcaa tcttacagcc atatcataag ctctcattcc agagataaat
     1261 atttcattta agtccattga atttaatttt tcaaaatcaa catcccagat ccaagaaaca
     1321 tccttaccat ctgcataatt atcatttaat aagaatgctg cagcaaagct ttctgaattt
     1381 agaagaagag tatctaaagc ttgattatat ccagctggat ttttaaccaa gataatccta
     1441 gcttctttat ctccaatttt tattgtttcc tgtcttccaa aagatgaact ttgattagag
     1501 aatgattttt gaataacttc atcattaata tgtaattctt ttgcaacaga ataagcacat
     1561 aatccgttgt atatattata agtaccaggt tgagttattg taaataaacc accatttata
     1621 acaaccttag aactttcagg ggtaagttct actatttctt ctactttata ttttaaatct
     1681 tctcttttga atccacagtt aggacagtag aaacttccaa gatgattgta tgttataaag
     1741 ttataagaat aaggttcttt acagaactta cagaacttag catcagcatt aatttctata
     1801 ttcttgtttt tatttggaga cacttcgaat ccataataaa ctttaggatt tttaacatca
     1861 agtcttccta ataaagattc atctccattt aaaagaagag tagtacttgg tactttaaca
     1921 actccttcta atattttatt tagagtagta taaacctctc catatctatc taactgatct
     1981 ctaaaaagat tagttacagt tattatttct ggagttatat actctgttat aaattttaag
     2041 ttagcttcat caacttcaat tactgcatat ctttctttat ttgaagagaa tttgtaatta
     2101 gctacgaaag tactaactat tcctggataa aggtttgctc cagtagcatt agttataaca
     2161 tctaatccat tacctttaag gacattatat atcattgaag tagtagtagt ctttccgtta
     2221 gttccagtaa ctaaaataac tttataattt ttagaaacta cttttaaaat gtttctatcg
     2281 atttttaaag ctactttacc tggaaagtta gttccacctt taaaaacttt ctttgaaata
     2341 aattgtgtca ttttagataa gattatgctt agataagatt taatagctat tttaaacacc
     2401 accttacaat tactttttag tcaatcttct taaactatta tagtatagca caaagtattt
     2461 aatttaccaa tatagtaatt tattaataat aagtaggaat tttttattat taaataatta
     2521 aatgtattta ttctatactt actagaaatt ttacaatgta atatatgtag aaatattgta
     2581 ttatatatag gaaggatata attaagtatc attaattata caaataaact agactagggg
     2641 gaatactaaa tgtatccaat aagatttgaa aacttatact atgataaagt ttggggtgga
     2701 agagatttcg aacttttcag agacaatatg ccagagggaa tgataggaga aacctgggat
     2761 gttgcttgtc ataaaaatgg aatgagtata gttgaaaatg gtgaatataa agggttaaga
     2821 tttgatgagt taatagataa attaggaagt gacttagtag gtactgaaat atcaaaagag
     2881 aaatttcctc tacttataaa attaataaac gctaaggata aattatcagt tcaagttcat
     2941 ccaaatgatg aatatggtat gagagttgaa ggagagcttg gaaaaactga aatttggtat
     3001 gttgttgaag cttttgaagg agctaattta atagtaggaa caaaggattg tactaaagaa
     3061 caatttgtta aagctataga aactggtgaa tttgatcagt atttaaatag agtagaagtt
     3121 aaaaaagggg atactttctt tgtaagaagt ggactagttc atgctatatg tgaaggtgtt
     3181 ataatagctg aaatccaaca aaatagtgat acaacctata gagtatatga ctataataga
     3241 ggaagagaaa tccatgttga aaaagcttta gattgtatcg atttctcatt aaaaggagaa
     3301 aaagctaagg gattaaaagt ttcatatgat gaatatgata aaacttatct ttcactatgt
     3361 gaatatttct cattagaaaa atatgacata catggagtat gtgaagaaga gagtgataaa
     3421 gaaagattct tcatatttac atgtgttgaa ggagaaggaa tcataacttc agaagaaggc
     3481 gaattagcaa ttaaaaaagg agatagtata ttcatacctg catctttagg aaaatattct
     3541 ttaaaaggaa acatgacatt attaaagagt tatgttccaa aagaaggtgc tgttgaagag
     3601 gaaatacttt cagtagtaaa agcttaataa gatttattat aagtttaaaa gcaaaaataa
     3661 atttaattgt ataatttaaa gaaagagtat atgaaaacta atttaaaata ataaatgatt
     3721 atgaattgct tttagaataa aaagcataaa gtaataataa tttacaagaa tcattaataa
     3781 aattagtttt agatatactc ttttatttat atttatgtaa aagtggacaa atttaactaa
     3841 tgaaattata ataaaaaagt aaaacttaat tagaaagtga gtgtaattat gggaattgat
     3901 tttttattca tacttaaggc cttaataatc gcaattgttg aagggcttac agagtttgtt
     3961 cctgtttcct caacaggaca catgattcta gttggtgacc ttattcactt taatacacaa
     4021 tcaggtggat tccctgaaat gtatgaagtt gttattcaat taggtgccat actagcagtt
     4081 gtagtattat attggagaaa gataagtagc tcagtagttg agtttttaag ttacatattt
     4141 tcatttatcg gattaaaaac atcagatgat aaaagaaaat atgaaaaaag acttgcagaa
     4201 tcaaaaactg gttttagatt tggtataaat gtaattatag ctactatacc agcagcgatt
     4261 ttagggttct tatttgatga tgagattaat aagtatttat tttcaactaa aactgtagct
     4321 ataggattta tagtaggtgg tatattactt atagttatag aaaacaactt tagaaaaaga
     4381 gctaaaagaa gtaagattgt aaaagatatt gataaaatga catatggaca atcattacta
     4441 gttggatgtt tccaatgttt atctttatgg cctggaatgt caagaagcgc atcaacaatc
     4501 atgggaggat ggatttctgg attaagtaca actgttgcta cagaatttac atttttctta
     4561 ggaataccag ttatggttgg agcatcagga ttaaaattat ttaaatttga ttattctcaa
     4621 atggatggaa cttatttaat tgccttaatc ttaggattta tagttgcatt tatagtttca
     4681 ttagttgtta tagataagtt tataaattac ttaaagaaaa aacctatgag agtttttgct
     4741 atatacagag tatttgctgg tatagtatta gctattttaa tatttactaa agtaattagt
     4801 taataaaaaa gacaaggata aaattccttg tcttttttta ttgaaagaaa taatagattt
     4861 tactaatata cgcgtaaaat tattataaat ttctaaaatt tagaaataat tttactttta
     4921 aaaaatatta ttatatgata tcatttactt agaagataat tgtaaaaaat ttacaaactg
     4981 tagttttaaa atgtttatta ggaggatttt atatgatgca accaattaag aaaatagcta
     5041 tattaaccgg aggaggcgat tgtcctggat taaatgcagt aataagagct gtaactagaa
     5101 ctgcaatctt aaaatatggg tatgaagtta taggttataa atttggatat agaggtctat
     5161 ataataatga ctttgtaaaa ttggatcttg attctgtttc tggaatactt catagaggag
     5221 gaacaatact tcatagctca aataaggaca acctatttga ttatcaagtt gaagatgaga
     5281 atggtaaaat agtaaaaaaa gacgtttctg atgttggtgt tgagaactta aagaaagaag
     5341 aagtagatgc tctagttgtt attggtggag atggaacctt aacctctgca agagattttt
     5401 cgagaaaagg tgtaaatgta ataggtgttc ctaaaacaat agataacgat ttattagcca
     5461 cagatgtaac ttttggattt aatactgcca cagaaatagc tacagaagct ttagatagac
     5521 ttcatactac agctgagtca catcatagaa taatgctttt agaagttatg ggaagaaatg
     5581 ctggttggat tgctttagac tcaggaatag ctggttctgc tgatgtaata ttattacctg
     5641 aaatacctta cgatataaac aaaatagttg aaaaagtaaa agaaagagaa gagtctggaa
     5701 aacagtttac aattattgta gttgcagaag gagcaaaacc taaagatgga gaagtagttg
     5761 tttctaaaat agttgatgat agtccagacc cagtaagact agggggaata gctaacaaat
     5821 tagctatgga tttagaggga ttaataaaga atcatgaaat aagaagcact gtgcttggac
     5881 atatacaaag agggggaaac acctctactt atgatagaat actttcaact aaatatggag
     5941 ttaaagctgt tgaacttata aatagtaatt tatttggaaa tatggttgca ttaaaaggaa
     6001 ataaagtatc ctatgaaagt ttagaaaatg taattggaaa tactaaaaat gtagatccag
     6061 aaggtgaatt ggtaaacact gctaaaagta taggaattag ttttgctgat taataatata
     6121 aaaacactca tcgaattaaa gtatgatgag tgttttcttt tctatttact aaaatttcac
     6181 ttataactta gtaaatcata agaatgaaga tatatcttaa aatgaaaaaa taaacattaa
     6241 caagaaaaaa tttacaatta atacttcaaa attttctgaa aatatggtac actttaaaca
     6301 aggcaacaat aaaagcaaca ttaaaagaaa gctggtgggt atttatgaaa gaagttcaaa
     6361 aatatttaga gagcagaatt ggttcgtata gctttttctt tgaagatttg aagagtggtt
     6421 atacttatgg ttataatgaa aacgttaaaa tgatttcagc aggttgtatg aaattaccta
     6481 tagctatagc tcttataaaa gaagtagagg aaggcaaaat agactttttg gataaagtta
     6541 aaatagaaag ttcagataag gtctatggaa ctggtataat acatgagttt ggagagaggg
     6601 attatactgt atttgaactt atggtagcaa tgctaattca aagtgataat acagcagcaa
     6661 ataaaataat tgatttagtc ggtatggata aaataaatga aactattaag gaatttggta
     6721 tgaaaaatac tgagcttaat agaaaaactg ccgatgaaag aaatttcaaa tcagatgtag
     6781 aaaattatac aacagcttat gatttaactt tagcctggaa aaaacttagt gagggttctt
     6841 tcttaagtaa agaaaactct caaatgctta tagatatact tacaagacaa cagattaaaa
     6901 acaaactagc tttatatata ccagataatc ttaagtttga tatatcaagt aaaacaggag
     6961 acaaaaaggg agttgagaat gatacagcct taattcatac tcaaaaagga aactttgctt
     7021 gtacaataat gtctagtgga attcctaatt cagtctacgg gactgtaacc ttggcaaagt
     7081 ctggtaaaat gacctgggac gccatcatga acaattggca ctaatattta atgtatttta
     7141 taaaacctta ataatagaac ttacaaataa tgtggggaga atatataatt attctaagat
     7201 ttaaattctt ataagaattt aattaattct caaaatgtgt ttgtaagttt tatttttaac
     7261 ttaaaataaa aaataattat taaaaacatt attgacataa aatttctaat atggtattat
     7321 aagcacaata aaagcagtcc agagaggctg aataaaataa cctttaaatt tgatccagag
     7381 aggtcaataa ggaagttata tagttgaatt ataatcgtaa aagatttatt tatacgtgaa
     7441 aagtcttcct tatcttagga agactttttt taataaaatt atatagcttt tcttctttaa
     7501 attaacacag agaggttaaa aaggagtgaa attttatgtt aaaaaacaaa cacttattag
     7561 atccatcaga tttcacaata gaggaatttg atgaaatttt caaattagca catcaaatta
     7621 tgacaaatcc aaaagaatat caaaacatat gcaatggtaa aattttagca acacttttct
     7681 atgagccaag cactagaaca agattgtctt ttgaatcagc aatgcttaga ctaggtggac
     7741 aagttatagg tttctcagaa ccaaattctt catcagtttc taaaggagaa tctttaagag
     7801 atactattaa aacattaaac tgctatgcag atcttatagc aatgagacat cctttagaag
     7861 gagcagcaaa agttgcttca atgtattcag atatcccagt tataaatgca ggagatggag
     7921 ggcatcaaca tccaactcaa acattaacag atcttcttac aataaaagaa tataaaggaa
     7981 acctagaagg aaatacaatt gcattatgtg gagacttaaa attcggtaga acagtgcatt
     8041 ctcttataaa agcactttca agatataaaa ataataaatt catcttaata tctccaatag
     8101 aacttagaat ccccaattat attagagagc aaattttaga aaaaaataat atagaataca
     8161 aagaaattac tagcctagaa gaaggaatta aggaagctga cattttatac atgacaagaa
     8221 tacaaagaga aagatttgta gatcaaagtg agtatgaaag attaaaagat gtatatgtat
     8281 tagatgaagc aaaaatgaag ggtgccaaag aagatatgat ggttcttcat cctcttccaa
     8341 gagtaaatga aatagcctat gaagtagatg aagattcaag agcattttac tttaagcaag
     8401 ccaaatgcgg aatgtatgta agaatggcct taatggcaaa acttttaggg gaggcgtaat
     8461 aggatgaaaa atagagaatt acaaaatcat aaatgtaaaa acacaaaatg tataactcaa
     8521 gtggaaaaat atgttcctca aagctttact ttaatagaca aaaagaataa tacatacaat
     8581 tgtgattatt gtaacgcaga aaacactttt caaaagcatt aaatttagga ggacataatt
     8641 aagatgaatt tgctaattaa aaatgtaaat ttaatagatg aaagcaacaa cttttttggt
     8701 gatatatata tagaaaaagg ggtaataaaa gaacttggaa ctgaactaaa taaagaatgc
     8761 gaaactctag atggaaaagg cttagtactt atgcctgcat ttatagatac tcatgcacac
     8821 tttagagatc caggctttga atataaagag gatattgaaa gtggatctaa ggctgcagtt
     8881 aggggtggat acacaacagt aaccttaatg ccaaacacaa aacccgtttg tagttcaaaa
     8941 gaaattttag attatgtggt taataagggt aaagaggtag acttagtaga tctatatcaa
     9001 acagtttcaa taacaaagaa tttatcaggt gaagaaataa atcatcttag agaatttgag
     9061 ggaaatccta atgttaaggc aataacagat gacggtaaag gggtgtcaga ttctaagatt
     9121 atgatggagg ctatgaaaat agctaaggaa aataactgga tagtaatgtc ccatgctgaa
     9181 agtccagaat tctcaaaagt tgatatgaga ttagctgaaa atatgatgac atggagagat
     9241 attacattag caaagtttat agattgtaga cttcacatgt ctcatgtaag tactaaggaa
     9301 gctatgaaat atataataga aggaaaaaat gatggagtta aagtaacttg cgaaataact
     9361 cctcaccatt tagctttaaa taataagatt agtaattata gagttaatcc tcctataaga
     9421 gaagaagagg atgtaaattt cttaataaag gcaataaaaa tgaactatgt tgattgtata
     9481 ggaacagatc atgctcctca ttcaaaggaa gataaggaaa aaggagcacc tggcatgatt
     9541 ggaattgaac aagctttctc aatatgttat accaagctag ttaaggaaaa tcacataagc
     9601 ttaaataagc taagtcaatt aatgagtgga aatgctgcta aattattaaa cttaaataaa
     9661 ggaaaacttc aaccaggttt tcttggagat ttagttctta tagatttaaa caagaaaaga
     9721 atattcaaag aagaagatat agtatctaga agtaaaaaca caccatttaa tggaatggag
     9781 ttttatggag atgtagtact aacaataaag aatggaaaaa tagtttacaa gggtgaattt
     9841 taggaggtat atatgatagc tgataaatta tttgaaaaag tagaaaaaaa cggagtagtt
     9901 tgtgttggac tagatacaag cttagattat ataccagagg agtttaaaag taagttttca
     9961 aatgaaagcg atatgttatt tgccttcaat aaagaaataa tagatgccac actagatgta
    10021 tctgcatgtt ttaaagttca aatagcttat tacgaagccc taggtcttaa gggattagag
    10081 gcttataaaa ataccttaag ttatttaaga gaaaagaatg ctttaatcat agctgatata
    10141 aaaagagggg atatagctgc aacggctaaa atgtacgcaa aggctcactt tgaaggggat
    10201 tttgaaagtg attttataac tctaaatcca tatatgggaa tggatagcat agatccatac
    10261 ttaccttata tagaaaaaaa tgaaaaagga gtatttgtac tagtaagaac ttcaaataaa
    10321 ggagctcagg atatagagta tctagaggct ggagatggaa agaaagttta tgatgtagtt
    10381 ggagaaaagt taaatactct aggaaaaaac tatttaggaa aacatggtta tagttcaata
    10441 ggtggagttg taggttgtac tcatcaagaa gaagcaaaag aaatgagaga taaactagat
    10501 acaatgccat ttttaatacc tggatatggt gcacagggag gaactgctaa agatgtagca
    10561 gcttatctta aaaatggaaa tggtggaatt gtaaactctt caagaaagat actattagcc
    10621 tataaagcta tggaagatag taaaaatttt gcagaatgtg ctagaaaaga agccatttca
    10681 atgagagaca gtataagaga agcgatttta aaatagataa aacacttggg aggacagatt
    10741 atggctatgg aatatttcaa aggtaaagta aaagaaaata ttgaattagt agagggaata
    10801 tactcactag tagtagaaca tgaagctaaa ataaatgcag gacaatttta tatgataaaa
    10861 actccaaata ctttcttagg aagaccaata agtgtttgtg aagtaaatgg gaatgatgta
    10921 cgctttgttt atgctactgt tggagccgga acaaatgaga tgaaaaaaat gatttctgga
    10981 gatgaaatag aaataatagg tcctttagga aatggtttcg atataaacaa agattatgga
    11041 agagttgcct tagtaagtgg aggtatagga acagctccaa tggtagaact tgcaaaagga
    11101 cttagaaaaa ataataaaga tattaaaatg gatttctatg gtggatttag agatgacata
    11161 tacttagtag atgaaatatc agaatatgta gacgaagtta aaatttcaac aaatacagga
    11221 aaacatggac ataaaggatt tgtaacagaa attcttcctc ttcaaaatta tgatacagtg
    11281 ctatgctgtg gaccggaaat catgatgaag aaggtagttg aaatgtgcaa agaagccaag
    11341 gttaatgtgt atatttccat ggaaaaacat atggcttgtg gagtaggtgc ttgcttagta
    11401 tgtacatgta aaacaaagtc aggaaataag agaacttgta aagatggtcc agtatttaat
    11461 ggattagaag ttgaattcta atttaggagg ggttaaaagt atgttaaaaa cagttataaa
    11521 tggagtagat tttaaaaacc ctgttatagc agcatcaggt actttcggct ttggagctga
    11581 gtacaataac ttctatgatg tgggagaatt aggaggaata agttcaaaag gattaactct
    11641 taagggtaga gaaggtaatg aaggaataag aatatatgaa actcctcaag gaataatgaa
    11701 ttcagtagga cttcaaaatc caggaataga gaaatttata gaagtagaac ttcctaaaat
    11761 gttagagcta ggaacaaata taatagccaa tgtaggggga gctactatag aggattatga
    11821 aaaagctgta tctcttttaa ataaagaaaa aatagacatg atagaactaa acatatcttg
    11881 tcctaatgta aaatcaggtg gaatggcctt tggaataaaa tcagacgtag cctatgaagt
    11941 ggtttctaag gtaagaaaaa tatgtgataa acctttaatg gtaaaattat caccaaatgc
    12001 tgaagatata tgcaatatgg catatagatg tgcagaggca ggagcagatt caatttcact
    12061 aataaataca ttaaaaggaa tggcaataga tataaaaaat agaaagccag tatttaataa
    12121 tgtgtatgca ggactttctg gcccagctgt aaaacctata gcacttagaa tggttcatga
    12181 agtagctaaa actgtagata ttccagtaat aggattagga ggaatttcta cagcagagga
    12241 tgccatagaa tttatgatgg caggagctaa agctatacaa ataggtacaa taaactttgt
    12301 aaatcctaaa gcaggtatag aaataataaa aggcttagaa gacttttgca aaaaagaagg
    12361 actaaaaaat attaatgaaa ttgtaggatg cataaagtaa taaaagatta aatagctaat
    12421 aaagattaca aatatatttt aaagattatt aaatcacaaa atttatcttc atattaatat
    12481 agttattttt atgaaaagta attttgaaga tattaaaaaa ataaataaga aagttttgga
    12541 ggaattaaat atgaaaaata cagacgctat ggttatagat acattaaaag aggttggagc
    12601 attattagaa ggacatttct tattatcatc aggaagacac tcaaatagat attgccaatg
    12661 tgctcaatta ttaaaatacc cagaaaaagc tgagaaagta ttaaaggttg tagctgatca
    12721 attaaaagat atagactttg acttagttgt aggaccagct atgggaggag ttatagtagc
    12781 ttacgaatta ggaagacaat taggtaaacc tgcaatcttt actgaaagag aaaatggaga
    12841 aatgactcta agaagaggat ttactataga aaaaggacaa aaggtagtaa taacagagga
    12901 tgttgtaact acaggtaaga gctttaagga agctgcaaag gtaattgaag aacaaggggg
    12961 agaagttgtt gcagtagttt gtatagtaga tagaactcca ggcaatgtaa cagatttccc
    13021 aatgtatagt tcaataaagc ttgatataga aagctttgag gcagaaaatt gcccactatg
    13081 taaagaagga gttccttaca taaagccagg aagcagaaat ataaaatagt ttaataaatt
    13141 tataaaaaaa ctctagctta taactagagt ttttttattt tagaaaatat attctaaaaa
    13201 cttatttaaa tcaattgatt atcctcattt tattacttat aatagaataa aatacattta
    13261 attacaataa agagaggata ttataagttg agtatatttt tgaagtataa aagtaaatta
    13321 atatttttct taacatttat aatagttaca cttactataa tagctatgat ttttgaaggt
    13381 ggtgttccta ttaataaacc atccattaaa ataacttata ataatgaaaa agtatcaaca
    13441 ataaatggag aatttaattg gtttaataaa ggagctggag gaaatagcac ttttgctgag
    13501 cctattgaaa atttaaagga gccattatat gcaaagggtg gagaaaagat aaaaataaga
    13561 ttctctaaag agccaaaatc tgtagttata catgacattt ctgtttctcc ttataaggat
    13621 tataaatttt tgaaagaaga atctaataaa gaattttcct ttatgcttcc tgaggaaaaa
    13681 ggagaatata attttcaggt ggatggtatt tgggattcaa ctcataatgt atctaaaata
    13741 tttaaagttt atatagatta acattgaata taataaataa aaaaatatta ttttggttaa
    13801 agagggatta tgaatttaga aaaagtgatt ttaaaaaaag atttaattaa ggatataaaa
    13861 agtatttctt cattatatga aagcgcagga tggtttgact atactgaaga tttaggaaaa
    13921 ttagaagagg catttaaaaa ttcattaaag ataatttcag catggcatga agaaaaatta
    13981 ataggtctta ttcgagttgt tggagatgga ctaactataa tatatataca agatatagta
    14041 gtacttcccg aatatcaagg taatggtatt ggtagaggtt tgattgattt tgtattagat
    14101 gaatataaac atgttagaca aaagattcta ataagtgatg ataaagaatc ttcaattgaa
    14161 ttttataaaa acataggttt taaatcaatt gatacttata atggagtagc ttttgtaagt
    14221 tatatagagt aaatttaaag caaccaaaaa tttaatttta ctttattcat ttaatttagc
    14281 ccccttgtta tttcttttaa ataattatat tacaattatt taaaattaaa taatgattta
    14341 tttaat
//
LOCUS       DAEVGI010000055        14248 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEVGI010000055 DAEVGI010000000
VERSION     DAEVGI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14248
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.055"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            129..371
                     /locus_tag="K8M77_001380"
     CDS             129..371
                     /locus_tag="K8M77_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085880.1"
                     /translation="MSVSKIKKQDSKKEKKIENFSQLKVRNIIKYSEDKKEDDFYEVM
                     KEGYKAMALINSQYAEEGTSVEYFDQIEYETWLCGV"
     gene            377..730
                     /locus_tag="K8M77_001381"
     CDS             377..730
                     /locus_tag="K8M77_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBI7085881.1"
                     /translation="MASLNVKRGDIFYADLSPVVGSEQGGIRPVIIIQNDIGNRYSPT
                     VIVAAITSQINKAKLPTHVEISSEEYGLNRDSVVLLEQIRTLDKKRLKEKIGHMTEED
                     MKKVNKSLLISLNLQ"
     gene            1167..1991
                     /locus_tag="K8M77_001382"
     CDS             1167..1991
                     /locus_tag="K8M77_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI7085882.1"
                     /translation="MSNKIQELKSMSKVIRKDIVTMLTESASGHPGGSLSIADIVTIL
                     YFDEMNIDPKNPKDPNRDRFVLSKGHAAPVLYSALARRGYFDPAELTTLRKFGSNLQG
                     HPNMNDLPGIDMSTGSLGQGISAAVGMALAGKLDNKDYRVFTILGDGELEEGQVWEAA
                     MSAAHYRLDNLTAFVDFNGLQIDGDIKEVMSPCPIDKKFEAFGWNVIVINGHDYEEII
                     NAIQKAKSTKGAPTCIVCNTVKGKGVSFMENEAAWHGTAPTKEQCEQAIKEIGGDN"
     gene            1991..2935
                     /locus_tag="K8M77_001383"
     CDS             1991..2935
                     /locus_tag="K8M77_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBI7085883.1"
                     /translation="MAKKIATREAYGKALAALANENKNIVVLDADLSKSTKTADFKKA
                     CPERFINMGIAEGNMMSVAAGLSTCGKIPFASTFAMFAAGRAFEQIRNSICYPRLNVK
                     ICATHAGLTVGEDGASHQAIEDLSLMRSIPNMTVICPSDAVETEAAIRAIAEYNGPCY
                     VRLGRAGVNVINDRPEYKFEIGKGIELKEGNDVTLFATGIMVDVAIEAVEALAKEGIN
                     ARLINIHTIKPIDSELILKAAKETGAIVTLEEHNIIGGLGSAVAEVVGGEYPVPVVRV
                     GVKDTFGESGKPDQLLKAYGLTSEEAVKAAKKAMSLKR"
     gene            3162..4019
                     /locus_tag="K8M77_001384"
     CDS             3162..4019
                     /locus_tag="K8M77_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBI7085884.1"
                     /translation="MKISMKTLKEYGIITLGVILLAISLEYFYYPNDIAAGGVSGLAL
                     VINKITGISQGMFMNVANVILFALAFWLIGGSFGGRSIYAAFGLSITISLIESFLPTF
                     ALTENMLLATIFGSVVGAFGIALVFSQNSSTGGTAIVAKILNKYLHIEIGKSLLCADC
                     VVVVCALLVFGVDKALFGLIGVFIMGILIDKIIEGFNSCKQLLIISEKSEEISQVIMK
                     EIERGCTKIEGTGGYSKEPVTILYVVVNRRQFISLKKKIKEIDPRAFITVNDSKEVFG
                     EGFLDLVEM"
     gene            4217..4903
                     /gene="ftsE"
                     /locus_tag="K8M77_001385"
     CDS             4217..4903
                     /gene="ftsE"
                     /locus_tag="K8M77_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBI7085885.1"
                     /translation="MIEFRNVSKVYNKNVKALTNVNINIDKGEFVFLVGPSGAGKSTF
                     IKMLLKEVEPSTGNIVMGNEDLSKIKRRQIPYHRRKIGMVFQDFRLIPTLNVYENVAF
                     AMRVVGASPKEIRKRVPMVLSLVGLSDKYKMFPNELSGGEQQRVSIARAIVNNPKVLI
                     ADEPTGNLDPETAKEIMELIDDINKAGTTVVMATHAKEIVNSMKKRVIAIDKGEVVSD
                     VQKGGYEYEV"
     gene            4893..5801
                     /locus_tag="K8M77_001386"
     CDS             4893..5801
                     /locus_tag="K8M77_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085886.1"
                     /translation="MKFSTIGYFISDAFKSLKRNRTISIASIATVLTTLFIFGAFLLT
                     ALNVKNGVTDVQDKVEVKVFLKDDVTQAQRDAVEAKLKTVQGVKEVEFESKDQAFAKM
                     EGSSDYNKEILAGYSKENNPLPASYIVRLESPEVASGVESAMKNNDKFMAGVEDVGND
                     EALIGTINSFVKVINTVGLVLGVVFVGVSLFLIVNTIKITVYSRRREVGIMKFVGATD
                     WFIRWPFVIEGIIIGLVGGILSTLLLFAGYNFLYEKIVSSSSLYMPQFVQPMYVLTTM
                     SWQFILAGIVIGAIGSIIALRKFLDV"
     gene            5890..7176
                     /locus_tag="K8M77_001387"
     CDS             5890..7176
                     /locus_tag="K8M77_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBI7085887.1"
                     /translation="MSHNKDEKNKYNNIIKNLKKKKGFRALAMGVGLTLIIGVSVYAG
                     NRLTAFGILPITSVSAVQSSLEKVNDTKNFKKVLEVREMLYRWYDGEIDDSKLAEGAI
                     KGMVSSLGDQYTYYMNGKEFSDFKEKSQGNYMGIGIQVAVKDGKIVVISPIQGGPAEK
                     AGIKTGDIILKINGEQVSGSELDKAVSMMKGATKENIKLTLYREGKGEFDVDVMRDVI
                     KTVNVKSEMINGDIGYIEVLAFDEGTAKDFETQLKALEEKGMKGLILDLRGNPGGFMK
                     ECVDLVSNFVPKDKVIVSTIDKYGNKEESVSKGGIAQGMPLVVLIDGGTASASEIVAG
                     AIRDYDLGTLVGTTSFGKGIVQVVLDKIGQEKDGTALKVTISKYYTPNGENIHKKGIG
                     PDVTVEYPKELKEKTYSRSTDPQFEKALEIIQEKIK"
     gene            7199..8479
                     /locus_tag="K8M77_001388"
     CDS             7199..8479
                     /locus_tag="K8M77_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085888.1"
                     /translation="MNLLIENIRGLSYALVSPTLSLMLIVIAIIFYFKNRKIAFMQKL
                     MLGRSIQSPLEMTLLQILIGILGGLIASLLLSILGVTFENSSLIDVILTVSVFSVLYK
                     NRFLKFPYIAALLGVIGVLVSKNIKYFGRGFSFSINLTSIIVLIGVFSIVEGILAMID
                     GDKGYLPIFTQKDGKLVGGFGFKRFWALPLCLLVVLGANSGNSIINEVKDLPQWLPFF
                     NGDKVRGLMSAAALGTLAAYGAISYEGTTFTQSKRSKTITSGLINIAYGIVVIILAYL
                     LKESLIFTISLIILIPLLYEFRIRVELKLESLREPLYFSNDDEICILDVLPNSIAHKK
                     GLRSGDKIVKINEEIPKNEKEVFMAIKRNFYGLDLEIRKNNGNIEKHTITGKDRGKQF
                     GIVLVPKGISFDKEIDEFLEKLKKASKDEEVKNK"
     gene            8567..10546
                     /gene="uvrB"
                     /locus_tag="K8M77_001389"
     CDS             8567..10546
                     /gene="uvrB"
                     /locus_tag="K8M77_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBI7085889.1"
                     /translation="MGEFKIQSKFKPTGDQPKAIDTLVQSIENGNRGQTLLGVTGSGK
                     TFTMANIIERTQKPTLILAHNKTLAAQLCAEFKEFFPDNIVEYFVSYYDYYQPEAYVP
                     QTDTFIEKDASINDEIDKLRHSATSALLERRDVIIVASVSCIYGLGNPEEYKKLTISL
                     RPGMIKDRDEVIKKLIEIQYERNDIDFARGTFRVRGDNLDIIPSSSSSKGIRIEFFGD
                     EIDRIREFDVLTGNIIGERQHVSITPASHFAASEETLEKSISIIEDELEDRLKVLTAE
                     DKILEAQRLKQRTNYDIEMIREMGYCQGIENYSRILDGRMPGTPPQTLLDYFPEDFLM
                     FIDESHVTLPQVRAMYAGDRSRKTSLVEFGFRLPCAFDNRPLKFSEFESKINQVVFVS
                     ATPGEYELDHSEIVAEQIIRPTGLLDPVIEIRPIKGQIDDLYGEIQRTVQRGFRVLIT
                     TLTKRMAEDLTKYLKDLNVKATYMHSDIDTLERMKIIRELRLGEVDVLIGINLLREGL
                     DIPEVALVAILDADKEGFLRSETSLIQTIGRAARNSESKVIMYADNITKSMDKSIKET
                     ERRRVIQMEYNEEHNITPTTVIKGVRDIIEATKVSEEKENYESEVKKAAKKDIPIEKL
                     IEQYEEEMKEAAKNLQFERAAELRDIIKDLKENSK"
     gene            10808..11659
                     /locus_tag="K8M77_001390"
     CDS             10808..11659
                     /locus_tag="K8M77_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBI7085890.1"
                     /translation="MAVKVITDSTSCIPKDLAEKYGVEIVSLSVIMNGESYKEVDIDS
                     EAFYDELAKCDKLPTSSQPPMDEFYNAFEKFAKDGHDIVAAFISSKLSGTYTTSHIIK
                     DMILEKYPDVKIDLIDSQTSVMALGIGVLEGAKAAQEGKDFDEVSKIVRNTIEESEII
                     FVPGTLENLKKGGRIGGAAALFGKMLKINPILTVKEGAVVVMDKVRTKKRAIDKIVEV
                     VKQDLKQKGIKQAVVCHILAEDEAKDLAKRLKEELDLDSMIGEISAVIGVHVGVKSVG
                     IAYARES"
     gene            11895..12620
                     /locus_tag="K8M77_001391"
     CDS             11895..12620
                     /locus_tag="K8M77_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085891.1"
                     /translation="MISLKEVNKEYTQGVKAVDNLNLEIKDGEIFGILGPNGAGKTTT
                     ISMITGITEPTMGEIKINNIDISKNPVEAKMQFGYVPDSPDMFLRYKGIEYLNFMGDV
                     YNVSESDREERIKDLAKRFEMTNALGDKIQSYSHGMRQKIIIMGVLIHKPSIWILDEP
                     LTGLDPKSSYILKEMMREHASEGNTVLFSTHVLEVAEKLCDRVGIIKKGKLIFVGTLE
                     ELKEEFKGDESLEKMFLEITENE"
     gene            12613..14211
                     /locus_tag="K8M77_001392"
     CDS             12613..14211
                     /locus_tag="K8M77_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7085892.1"
                     /translation="MNRIKLLLKYFIGNSFYNASSNIKSRNLAIVMYILIIIGISTPI
                     ANMIDSMYPNFASIGQEGYLLSIIFLIGSLTLLVLGVYDILDSFFFSQDIEPLMPLPF
                     KSGEIMIGKFITCLVDMYIYLSITIIPLISFGLNAKMGFSYFLMIIPVYLFAPIIIVI
                     FCILITMILMSFINVSKYQNAFKIIFGTLGIVLILGVYSLNSTGLNSENVSVALKNKT
                     ALVDLTNKIFITTTFSVKALLYSNSLKGLLNLGLLILISIVALIIAYFLGKLMYKKIL
                     SRNLNVYSERKNILENNKDKVVVRSSVRKAMVLRELRTILRDPSNFINCVVMIVYMPI
                     FIFIFFIKGNILAGDTQLAKDTIIMAATFIVTALTIAGNSVASTALSREGKEIFISKY
                     IPVDYKVQIQSKLIVSFIVNGLALILGIAILIYLKASPLVIVMSLLVQMGTIVAISLW
                     GMILDYLSPKLEWTDTKNLYSKNFKPLLVMLICLVIGGFNIGLIVTKSPIIVFLIDIS
                     ILLIVSLILYKILMKKGLEAYKRL"
BASE COUNT         5605 a         1484 c         2666 g         4493 t
ORIGIN      
        1 aatttaataa taaatattat gtcaaaaaat atcattttca taagatgaaa tctttgacaa
       61 ggtggcatat tttgtaatat aatctataca taattttata actcttttta catttaggag
      121 tgatgtacat gtcagtatca aagattaaaa aacaagattc aaaaaaagaa aaaaaaatcg
      181 aaaattttag tcaattaaaa gtaagaaata taataaaata tagtgaggac aaaaaagaag
      241 acgactttta tgaagtaatg aaggaaggat ataaagctat ggctttaata aattcacaat
      301 atgctgaaga aggaacttct gtagaatact ttgatcaaat agaatatgag acatggcttt
      361 gcggagtgtg atataaatgg ctagtttaaa tgtaaaaaga ggggatatat tttatgctga
      421 tcttagtcca gttgttggat cagagcaagg tggaataaga ccagtcataa taatacaaaa
      481 tgatatagga aacaggtata gtccaactgt aatcgttgcg gctataactt cacaaataaa
      541 taaggcaaaa ttgcctacac atgtagaaat ttcttcagag gaatatggat taaatagaga
      601 ttctgttgtt cttttagaac aaataagaac tttagataag aagagattaa aagagaaaat
      661 tggacatatg acagaggaag acatgaaaaa agtaaataaa tcgctgttga taagtttaaa
      721 tttacaataa ttagataact taggctatga ttaaatattt aatcatagcc ttaatttttt
      781 actcttaaaa aggaattttt caatactttt tagataataa tatataaaat ttaaaaaata
      841 attaactgta atcgatataa tttctgtata gtattaaagc tataaatgtg atactctaat
      901 taaaactttt tcatataaaa atttatccag gatatactat tatattattt aatattaaaa
      961 aaataagtat aagctacaaa atttgaagag aaatagaata ctctaatagg gtattagagt
     1021 attcttaatg aaagcaaaaa gtagaaaaag attagcataa ataaaaaatt actatatgaa
     1081 aaatcaaatt tttatgatag aatgaatttg gtaaaaaggt aaaataatat tttaaaaaag
     1141 tttacataaa tgtacaggag gtcattatga gcaataagat acaagagtta aaaagcatgt
     1201 ctaaggttat aagaaaagac atagtaacta tgcttactga gtcagcatca ggacatccag
     1261 gtggatcttt atcaatcgca gatatagtaa caattttata cttcgatgaa atgaatattg
     1321 atccaaaaaa tccaaaggat ccaaatagag atagattcgt tttatcaaaa ggacatgcag
     1381 caccagtatt atatagtgct ttagcaagaa gaggatattt tgatccagca gaattaacaa
     1441 cattaagaaa atttggttca aaccttcaag gacatccaaa tatgaacgac ttaccaggaa
     1501 tagatatgtc aactggttct ttaggtcaag gtatatcagc ggctgtaggt atggcattag
     1561 ctggtaaatt agacaacaaa gattacagag tatttactat tttaggagat ggagagttag
     1621 aagaaggtca agtttgggaa gcagctatgt cagcagctca ctacagacta gacaacttaa
     1681 ctgctttcgt tgacttcaat ggattacaaa tagatggtga cattaaagaa gtaatgagtc
     1741 catgtccaat agacaagaaa ttcgaagctt tcggatggaa tgtaatagtt ataaacggac
     1801 atgattatga agaaataata aatgctatac aaaaagctaa gagtacaaaa ggagcaccaa
     1861 cttgcatagt ttgtaacaca gtaaaaggta aaggtgtttc atttatggaa aatgaagcag
     1921 cttggcatgg aacagctcca actaaagagc aatgtgagca agctataaaa gagatcggag
     1981 gggacaacta atggcgaaaa aaatagcaac tagagaagca tacggaaaag cattagctgc
     2041 tttagctaac gaaaataaga acatagtagt tttagacgca gacctttcaa aatcaactaa
     2101 aactgctgat ttcaaaaaag catgcccaga aagattcata aatatgggta ttgcagaagg
     2161 aaatatgatg tcagtagcag caggtttatc aacttgtgga aaaattccat ttgctagtac
     2221 atttgctatg tttgctgctg gaagagcatt tgagcaaata agaaactcaa tatgttaccc
     2281 aagattaaat gtaaaaatat gtgctactca tgctggttta acagttggtg aagatggagc
     2341 atcacaccaa gctatagaag atttatcttt aatgagaagt ataccaaaca tgacagtaat
     2401 atgcccatca gatgcagttg aaacagaagc tgcaataaga gctattgctg aatacaatgg
     2461 accttgctat gtaagattag gaagagcagg agttaatgta ataaatgata gaccagagta
     2521 caagtttgaa attggaaaag gtatagaatt aaaagaaggt aatgatgtaa cattatttgc
     2581 aacaggtata atggttgatg tagctataga agctgttgaa gctttagcta aagaaggaat
     2641 taacgctaga ttaataaaca ttcatactat taaaccaata gattcagagc ttatattaaa
     2701 agctgctaaa gaaactggag caatagttac attagaagag cataatataa tcggaggatt
     2761 aggatcagct gtagctgagg ttgttggagg agaatatcca gtaccagtag ttagagttgg
     2821 tgttaaagat acattcggag aaagtggtaa accagaccaa ttattaaagg cttatggatt
     2881 aacttcagaa gaagcagtta aggctgctaa gaaagcaatg agcttaaaaa gataatctat
     2941 aagatataaa aaagaagcag catgagtaat ttttctcatg ctgctttttt ctttatacat
     3001 aaaaatacaa aaaaagtatt aatttgaatt ttattgccaa aatatgaata taaatgttaa
     3061 tattaaaaat tgagggcaat aatataaatg atgaattaat aattaattta aaatggggag
     3121 agcaaattaa atataaatct atttaagagg gggtttttaa aatgaagatt agtatgaaaa
     3181 cattaaaaga gtatggaata attactttag gagtaattct tttagctata tctttagaat
     3241 acttttatta tcctaatgat attgctgctg gtggagtatc gggtttagct ttagttatta
     3301 acaagataac aggtatatca caaggaatgt ttatgaatgt tgctaacgtt atattatttg
     3361 ccttagcatt ttggcttata ggtggaagct ttggagggcg tagtatatat gcagcctttg
     3421 gattatccat aacaataagt ttaatagaaa gctttttacc aacttttgca ttaacagaaa
     3481 atatgctttt agctactatc tttggtagcg tagttggagc ctttggaata gcattagtgt
     3541 ttagccaaaa ttcttcaaca gggggaacgg ccattgtagc taaaatatta aataaatatt
     3601 tacatattga aataggaaaa tcacttttat gtgcagattg cgttgttgta gtatgtgcac
     3661 ttctagtatt tggagttgat aaagccttat ttggattaat tggtgttttt ataatgggaa
     3721 tattgattga taaaataata gaaggtttta attcatgtaa acaactttta ataattagtg
     3781 aaaaaagtga agaaatttct caggtaataa tgaaagaaat tgaaagagga tgtactaaaa
     3841 tagaaggtac tggaggatat tcaaaggaac ctgtaacaat actttatgta gtagtaaata
     3901 gaagacagtt tataagtctt aaaaagaaaa ttaaggaaat agatccaaga gcctttataa
     3961 cagtaaatga ttctaaagaa gtctttggag aaggttttct tgatttagta gaaatgtaga
     4021 actttgttat gatataatta agatgcttta cattaaggta tgaaatatta acaaaaatta
     4081 agtagattaa tgtagaaaag tagggtaaaa tagattagtg ttgatagcaa tcgggtaaat
     4141 ttatatatat tttttctaaa tatttgttta gattttaatt tgttgagtag aaaaaagtta
     4201 ttaaggagtg ttaacaatga tagaatttag gaatgttagc aaggtctata ataagaatgt
     4261 caaagcactt acaaatgtca atataaatat tgataaaggt gagtttgtat tcttagttgg
     4321 accgagtggt gctggtaagt caacttttat aaaaatgtta ttaaaagaag ttgaaccatc
     4381 aactggaaac atagttatgg gaaatgaaga tttatctaaa ataaaaagaa gacaaatacc
     4441 gtatcataga agaaaaatag gtatggtatt tcaagacttt agattaatac cgactcttaa
     4501 tgtttatgaa aatgttgcat ttgcaatgag ggtagtaggg gcttcaccaa aagaaataag
     4561 aaagagagtt ccaatggtat tatcactagt tggattatct gataaataca aaatgtttcc
     4621 aaatgaatta tcaggaggag agcaacaaag ggtttcaata gcaagagcta tcgttaataa
     4681 tccaaaagtt ttaatagctg acgaaccaac aggtaactta gatccagaaa cagctaaaga
     4741 aattatggaa ttaatagatg atataaacaa ggctggaaca acagtagtta tggctactca
     4801 tgctaaagaa atagttaact caatgaaaaa gagagttata gctatagata aaggtgaagt
     4861 agttagtgat gttcagaaag gaggatatga atatgaagtt tagtactata ggatatttca
     4921 tatcagatgc tttcaaaagt ttaaagagaa atagaacaat aagtatagct tctatagcaa
     4981 ctgttcttac tacattattt atatttggag catttttatt aactgcttta aatgttaaaa
     5041 atggggttac agatgttcaa gacaaagttg aagttaaggt tttccttaaa gatgacgtaa
     5101 cacaagcgca aagggatgca gttgaagcaa aacttaagac tgttcaaggg gtaaaagaag
     5161 tagaatttga atctaaagat caagcctttg caaagatgga aggttcaagt gattataata
     5221 aagagatttt agctggatat agcaaagaaa ataacccatt accagcttca tacatagtaa
     5281 gattagaaag tccagaagta gcttcaggcg ttgaaagtgc tatgaaaaat aatgataaat
     5341 ttatggctgg cgtagaagat gtaggtaatg atgaagcact tataggtact ataaactcct
     5401 ttgtaaaagt tattaataca gtaggattag ttttaggtgt agtattcgta ggtgtatcac
     5461 tattcttaat agtaaataca ataaagataa cagtttattc aagaagaaga gaagtaggaa
     5521 taatgaaatt cgttggagca accgactggt ttataagatg gcctttcgta atagaaggga
     5581 ttataatagg acttgtaggt ggtattcttt caacactttt attattcgca gggtataact
     5641 tcttatatga aaaaattgta tcttcatcat cactatatat gccacaattt gtacaaccaa
     5701 tgtatgtttt aacaacaatg tcatggcagt ttatactagc aggtattgtt attggtgcaa
     5761 taggtagtat aatagcttta agaaaattct tagacgttta atatctaata tagaatataa
     5821 aaagcttaaa atacgtaaac tatttaaata tagcgtattt taagcttttt tttgaaaggg
     5881 tgggttttta tgagtcacaa taaggatgaa aaaaataaat ataataatat tattaaaaat
     5941 cttaagaaga aaaaaggatt cagagcttta gccatgggtg ttggtttaac tttaataatt
     6001 ggtgtttctg tatatgcagg aaatagacta actgcctttg gaatcttgcc tataaccagt
     6061 gtaagtgcag ttcaatcttc cttagagaaa gtaaatgata caaaaaattt taaaaaggta
     6121 ttagaagtaa gggaaatgct ttatagatgg tatgatggag aaattgatga tagcaaatta
     6181 gctgaaggtg ctataaaggg tatggtttca tctttaggtg atcaatacac atattatatg
     6241 aatggaaaag aattttcaga ttttaaagaa aaaagtcaag gaaactatat gggaattggg
     6301 attcaagtag ctgttaagga tggtaagata gtagtgattt ctcctattca aggaggacca
     6361 gctgaaaaag caggaataaa aactggagat attatcttaa aaataaatgg agaacaagtt
     6421 tcaggaagtg aattagataa agccgtttca atgatgaaag gtgctacaaa agaaaatata
     6481 aaattaacat tatatagaga aggtaaaggc gaatttgatg ttgatgttat gagagatgta
     6541 attaaaacag ttaacgttaa aagtgaaatg attaatggag atattggata tatagaagtt
     6601 ttagcttttg atgagggaac agctaaagac tttgaaactc aattaaaagc tttagaagag
     6661 aaaggtatga agggattaat ccttgattta agaggaaatc caggaggatt tatgaaagaa
     6721 tgtgtagatc tagtatcaaa ctttgttcca aaagataaag ttatagtgtc tacaatagat
     6781 aagtatggta ataaagagga aagtgtatct aaaggcggaa ttgcacaggg aatgccttta
     6841 gtagttttaa ttgatggagg tactgcttct gcctcagaaa tagtagcagg agctattaga
     6901 gattatgatt taggaactct agttggaaca acatcctttg gtaaaggaat agttcaagtt
     6961 gttttagata agataggtca agaaaaagat ggtacagctt taaaagtaac tatttcaaaa
     7021 tactatactc caaatggaga aaatattcac aaaaagggta taggaccaga tgttacagtt
     7081 gaatatccaa aagagcttaa agaaaagaca tattcaagaa gtactgatcc tcaatttgaa
     7141 aaagccttag aaatcataca agaaaagata aaataaataa atttttggag gacttaaaat
     7201 gaatttactt atagaaaata taagaggttt atcttatgca ttagtaagtc ctaccttgtc
     7261 tttaatgtta atagtaatag ctataatatt ttattttaaa aataggaaga tagcttttat
     7321 gcaaaagtta atgcttggga ggagtattca atctcctctt gagatgactt tacttcagat
     7381 attgattggt atattaggag gactaatagc tagtttatta ctaagtattt taggagtaac
     7441 ctttgaaaat agttctttaa tagatgttat tttaacagta tcagtatttt ctgttttata
     7501 taaaaataga tttcttaaat tcccttatat agcagccttg ctaggggtta taggagtttt
     7561 agtaagtaaa aatattaaat attttggaag gggctttagt ttttctatta atttaacttc
     7621 aattattgtg ttaataggtg ttttttcaat agttgagggt atcttagcaa tgatagatgg
     7681 agacaaaggt tatttaccta tctttactca aaaggatgga aagttagtag gtggatttgg
     7741 ctttaaaaga ttttgggcac tgcctttatg cttattagtt gttttaggag ctaatagtgg
     7801 aaattcaatt attaatgaag tcaaagattt accacagtgg ttaccatttt ttaatggaga
     7861 taaagtaaga ggactaatga gtgctgcagc tttaggaacc ttagcagctt atggagcaat
     7921 ttcatatgaa ggaactacgt ttactcaaag caaaagaagt aagaccataa catctggatt
     7981 aattaatatt gcatatggta tagtagttat aatattagct tatttattaa aagaaagcct
     8041 tatttttact atttcactaa ttattttaat tccattatta tatgaattta gaataagagt
     8101 ggaactaaaa ttagagtctc ttagagagcc tttatatttt tcaaatgatg atgaaatatg
     8161 cattttagat gttttaccaa attctattgc acacaaaaag ggattaagaa gtggagataa
     8221 aatagttaaa attaatgaag aaatccctaa gaatgaaaaa gaagtattta tggcaataaa
     8281 gagaaatttt tatggtttag atcttgaaat aagaaaaaat aatggaaata ttgagaagca
     8341 tacaattaca ggcaaggata gaggaaagca gtttggcata gtgttagttc ctaagggaat
     8401 atcctttgat aaagagatag atgaattttt agaaaaattg aaaaaagcca gtaaggatga
     8461 agaagtaaaa aataaataat ggttaacttg gctgtatcta agattcacct attaaggtga
     8521 gaagtgtttt aagataccgc cttttatagt taggagggaa aaaattatgg gagaatttaa
     8581 aatacaatca aaatttaaac ctacagggga tcagcctaaa gcaatagata ctttagttca
     8641 gtctattgaa aatggtaata gagggcaaac tcttttagga gttacaggct caggaaaaac
     8701 attcactatg gcaaatatca tagaaagaac tcaaaaacca acccttattt tagctcataa
     8761 taagacattg gcagctcaac tttgtgctga atttaaggaa ttctttcccg acaatatagt
     8821 agagtacttt gtttcatatt atgattacta tcaaccagag gcttatgtac ctcaaacaga
     8881 tacttttata gagaaggatg catctattaa tgacgagatt gataaacttc gtcactcagc
     8941 tacttctgca cttttagaga gaagagatgt aataatagtt gcttcagttt cttgtatata
     9001 tggacttggt aatccagaag agtataaaaa gcttacaata tctttaagac cgggtatgat
     9061 taaggacagg gatgaagtta ttaagaagct tatagaaata caatatgaaa gaaatgacat
     9121 agattttgct agaggaactt ttagagtaag aggagataat ttagatataa ttccttcatc
     9181 atcttcttct aagggtatta gaattgaatt ctttggagat gagatagata gaataagaga
     9241 gtttgatgtt cttacaggaa atataattgg agaaagacaa catgttagta taactccagc
     9301 gtctcacttt gctgcctctg aggaaacctt agaaaaatca ataagtataa tagaagatga
     9361 acttgaagat agattaaaag ttcttacagc tgaggataaa atattagagg cacaaaggtt
     9421 aaagcaaaga acaaattatg atatagaaat gattagagaa atggggtatt gccaaggaat
     9481 agaaaattat tcaagaatat tagatggaag aatgccggga actccacctc aaactttact
     9541 tgattatttc ccagaagatt tcttaatgtt tatagatgaa agccatgtta cactacctca
     9601 agttagggca atgtatgctg gagatagatc tagaaagaca tctttagttg agtttggatt
     9661 tagactacct tgtgcatttg ataatagacc tcttaaattc agtgaatttg aaagcaaaat
     9721 aaatcaagta gtctttgtaa gtgctactcc aggagagtat gaactagatc attctgagat
     9781 tgtagcggaa caaataataa gacctacagg gcttttagat cctgtaattg aaataagacc
     9841 tataaaggga caaattgatg atctttatgg tgagattcaa agaactgtac aaagaggatt
     9901 tagagttctt ataacaactc ttacaaagag aatggctgag gatttaacta agtatttaaa
     9961 agacttaaat gttaaagcta catacatgca ttcagatata gataccttag aaagaatgaa
    10021 gataataaga gaacttagac ttggagaggt tgatgtatta ataggtataa atctcttaag
    10081 ggaaggttta gatatacctg aggtagcttt agttgcaata ttagatgctg ataaagaagg
    10141 attcttaaga tctgaaactt cattaataca aaccataggt agagcggcta gaaactcaga
    10201 aagtaaagta ataatgtatg cagataatat tacaaaatct atggataaat ctattaagga
    10261 aactgaaaga agaagagtta ttcagatgga gtataatgag gaacataata ttactcctac
    10321 tactgtaata aaaggagtta gggatataat tgaagctact aaggtttcag aagaaaaaga
    10381 aaattatgaa agtgaagtta aaaaggcagc taagaaagat attcctatag aaaaacttat
    10441 agagcaatat gaggaagaaa tgaaggaagc ggctaaaaat cttcaatttg aaagagctgc
    10501 tgaacttaga gatataataa aggatttaaa agaaaactct aaataaatat ttttagcttt
    10561 gtttacataa ttgtgtttat taaagtaatt attgtattga aaagagtttt acaaattaaa
    10621 attagatgtt aaaatgaata atagattata aaaataaata caaaaagaga taaatattat
    10681 aatgtaaata aaaaataagt atataattaa atggaatatt agagttaatt atatatatga
    10741 tagtttttaa ttttataaga aaatctaaaa gagctaaaag aatgtattta agttttagga
    10801 ggaatatatg gctgttaaag taattacaga tagcactagt tgcataccaa aagatttagc
    10861 agaaaagtac ggagtagaga tagtatcttt aagcgttata atgaatggag aaagttataa
    10921 ggaagttgat atagatagtg aagcttttta tgatgaatta gctaaatgtg ataaacttcc
    10981 aacctcatct caaccaccaa tggatgagtt ttataatgct tttgaaaaat ttgctaagga
    11041 tggtcatgat atagttgcag cgtttatatc atctaaatta agtggaacat acacaacatc
    11101 acatataata aaagatatga ttttagaaaa atatcctgat gttaagattg atcttataga
    11161 ctctcaaact tcagtaatgg cacttggaat tggtgtgtta gaaggagcaa aggcggctca
    11221 agaaggaaaa gactttgatg aggtttcaaa aatagttaga aatactatag aagaatcaga
    11281 gataatattt gttccaggta ctttagagaa ccttaaaaaa ggtggcagaa taggtggagc
    11341 agcagcttta tttggaaaaa tgcttaaaat aaatccaatt ctaacagtta aagagggcgc
    11401 tgttgttgta atggataaag ttagaactaa aaagagagca atagataaga ttgtagaagt
    11461 tgttaaacag gatttaaagc aaaagggaat aaagcaagca gttgtatgtc atatattagc
    11521 tgaagatgaa gctaaagatt tagctaaaag acttaaagaa gaattagatt tagactctat
    11581 gattggtgaa ataagtgcag ttataggtgt acatgtagga gttaagagtg taggtatagc
    11641 ttatgctaga gaatcttaat taaatataat ttaaaaagaa gcttgataaa tttatataag
    11701 tttatcaagc ttctttttat tattttacat aaatagagga tatatttatg ttgaattttt
    11761 atctaaatat aaactaaaat taaagacttt attaagcatt aaggtttttg tggttaaaat
    11821 tgtatctgaa actataatat gtatatatat tgtgttaaaa taatgttact aaaaggttaa
    11881 ggaggattgg aatattgatt agtttaaaag aggttaataa ggaatacaca caaggagtaa
    11941 aagctgtaga taatttaaat ttagaaatta aagatggtga gatatttggt atattaggac
    12001 caaatggagc tgggaagact actacaataa gtatgattac aggaataaca gagcctacta
    12061 tgggagaaat aaaaataaat aatattgata taagtaaaaa tcctgtagaa gctaaaatgc
    12121 aatttggata tgttcctgat agtccagata tgtttttaag atataaaggt atagagtatc
    12181 ttaattttat gggagacgtt tataatgtta gtgaatcaga tagagaagaa aggattaaag
    12241 acttagctaa gaggtttgaa atgactaatg ctttgggaga taaaattcaa agttattctc
    12301 atggaatgag acaaaaaata ataataatgg gtgttttaat tcataagcct agtatttgga
    12361 tattagatga acctttaact ggattagatc caaaatcctc atacatatta aaagagatga
    12421 tgagagaaca tgcaagtgaa gggaatactg tattattttc tacacatgtc ttagaagttg
    12481 cagaaaagtt atgtgatagg gtaggcataa taaaaaaagg gaaacttatt tttgtaggta
    12541 cattagaaga gttaaaagaa gaatttaaag gtgatgaatc actagaaaaa atgttcttgg
    12601 agattactga aaatgaatag aattaaatta ttattaaaat attttatagg aaactctttt
    12661 tataatgctt ctagtaacat aaaatctaga aatctagcta tagttatgta tatcttaata
    12721 ataataggaa tatctacacc tatagctaat atgatagatt ctatgtaccc taactttgct
    12781 agtattggac aagaaggata tcttctttca attatatttt taattggatc actaacttta
    12841 ttggttttag gagtttatga tattttagat tcatttttct tttcacagga tatagaaccc
    12901 ttaatgccat taccatttaa aagtggtgaa ataatgatag gaaaattcat aacatgttta
    12961 gtggatatgt atatctattt aagtattaca ataattccct taatatcttt tgggctaaat
    13021 gctaaaatgg gattttctta ttttcttatg attattccag tttatttatt tgctccaatt
    13081 ataatagtaa tattttgcat attaataact atgattttaa tgagtttcat aaatgtatct
    13141 aaatatcaaa atgcttttaa aataatattt ggtacattag gaatagtctt aattttagga
    13201 gtatattctt taaattcaac aggattaaat tcagagaatg tttcagtggc attaaaaaat
    13261 aagacagcat tagtggattt aactaacaaa atattcataa ctactacctt tagtgtaaaa
    13321 gcattacttt atagcaattc tttaaaggga ttattaaatt taggcctttt aattcttata
    13381 tcaatagtag ctttaataat tgcatatttc ttaggaaaat taatgtataa aaagatacta
    13441 agtagaaatt taaatgtata tagtgagaga aagaatatac tagaaaataa taaggataag
    13501 gttgttgtaa ggagttcagt aagaaaagca atggtattaa gagagcttag aacaatatta
    13561 agagatccca gtaactttat aaactgtgtt gttatgattg tttatatgcc tatatttata
    13621 tttattttct ttattaaagg aaatatttta gcaggggata ctcagttagc aaaggataca
    13681 ataataatgg cagcgacttt tatagttaca gccttaacca tagcaggtaa ctcagttgca
    13741 tcaacagcct taagtagaga aggaaaagaa atatttatat cgaaatatat tccagtagat
    13801 tataaggtac agattcaatc taagcttata gtttctttca tagtaaatgg attagcactt
    13861 atattgggaa tagctatatt aatttattta aaggcatctc cattggttat cgtaatgagt
    13921 ttattagtac aaatgggaac catagtcgca atatctttat ggggaatgat tctagattat
    13981 ttatcaccta agttagaatg gacagataca aagaatttat atagtaaaaa ttttaaacct
    14041 ttattagtaa tgttaatttg cttagtaata ggaggattta atataggttt aattgttacc
    14101 aaaagtccaa taatagtatt tttaatagac ataagtattc tgttaatagt aagcttaatt
    14161 ttatataaaa ttcttatgaa aaaaggatta gaggcttata aaaggttata atataataat
    14221 ttataaaaac gtaacctttt tataaata
//
LOCUS       DAEVGI010000056        14223 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEVGI010000056 DAEVGI010000000
VERSION     DAEVGI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 14223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14223
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.056"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            418..780
                     /locus_tag="K8M77_001393"
     CDS             418..780
                     /locus_tag="K8M77_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBI7085893.1"
                     /translation="MNAKIKSLVFSIIKTNILLTIIATLLISLFNFRFGVAFLVGSIV
                     ATINFSVNAIVIHKVINKRRGALKIQLSFVLRMMLILLFTVIFMKDVKVLAFYLIGFI
                     SHQISIFIYTKKNKALSN"
     gene            839..1513
                     /locus_tag="K8M77_001394"
     CDS             839..1513
                     /locus_tag="K8M77_001394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBI7085894.1"
                     /translation="MDIFAPMFTVKVGSLTISSTIVFQWAAMLVITIFAILSTKNLKN
                     KPGKLQVVLESLYQTVYNLVKSNMGESYIGFVPYIGTLAVFLLVLNLYGLIGLAPPTQ
                     DLSVAVALTITSFVVVHVNAIRKCHVGGYIKGYFKPYLVMLPLNLMERIIFPVSLALR
                     LFGNMLAATLLIDLMYSGLAHIGWIAQLGVPIVAHGFFDVFDGTIQMIVFTMLTMVNI
                     KMTAEH"
     gene            1574..1792
                     /gene="atpE"
                     /locus_tag="K8M77_001395"
     CDS             1574..1792
                     /gene="atpE"
                     /locus_tag="K8M77_001395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBI7085895.1"
                     /translation="MDMKLLAAGIAVLAGIGAGIGIGIATAGAIEATARQPEASDKIQ
                     SLFIMGAGLSEATAIYGLVISIILLFVV"
     gene            1891..2370
                     /locus_tag="K8M77_001396"
     CDS             1891..2370
                     /locus_tag="K8M77_001396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBI7085896.1"
                     /translation="MELNFMRILATIINFIILILILKHFFWDKIKRAIDARQEAIDET
                     ILKADEDAEKARRLRLDNERILKSAKEDGRKLREEQKKEADRIYKEIVDDAHREAESI
                     INRANIEIQREEEKVKYELKQQVVDISVMLSEKALGESIDESKHRELINDFIEKVGI"
     gene            2373..2912
                     /locus_tag="K8M77_001397"
     CDS             2373..2912
                     /locus_tag="K8M77_001397"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBI7085897.1"
                     /translation="MYEYLDRRYALALYEVAEEKNKVEEYLNDLREICEIIYGNNELY
                     EVIKHPQISTVRKKKTFRNIFEGKIDDELLSFLMVLIEKDRILYLREKLKEMEKIHLE
                     RNNTLLAEVKSVVPLTEDEVTRLVEKLENKYSKKILLKQEIDRSIIGGLYVRVGDDVI
                     DGTVKSRLDDMKQIMLKRE"
     gene            2923..4431
                     /locus_tag="K8M77_001398"
     CDS             2923..4431
                     /locus_tag="K8M77_001398"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBI7085898.1"
                     /translation="MHIKPEEITSIIKNEIKNYEKELETVDSGTIIQIGDGVARVYGL
                     EECMEGELLEFPNQVFGMALNLEQDNVGCVLLGSEEGIKEGDIVKRTGKIVEVPVGED
                     LIGRVVNSLGQPIDGKGPIKNDGYRAIEVPAPGILERSSVNEPLQTGIKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGKDVICIYVAIGQKQSTVANIVNTLTEEGAM
                     DYSIVVTASASESAPLQYIAPYSGCTMGEYFMNKGKHVLIIYDDLSKHAVAYRTMSLL
                     IRRPPGREAYPGDVFYIHSRLLERAAKLSKENGGGSLTALPIIETLAGDVTAYIPTNV
                     ISITDGQIFLETELFNAGQRPAVNPGISVSRVGGNAQIKAMKQVSGTLRLELAQYREL
                     ASFAQFGSDLDKDTQARLEKGKRLIEILKQDQYKPMAVEKQIMIIYAAVNNFLLDIKV
                     SDIKKFEKEFLEYMDTHHREIGKAILDKKVLDDELKSALESAIVEFKKIFLM"
     gene            4496..5347
                     /locus_tag="K8M77_001399"
     CDS             4496..5347
                     /locus_tag="K8M77_001399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBI7085899.1"
                     /translation="MAGAGLLEIKRRIKSIKNTRKITKAMGLVATSKLRKARQKLTEN
                     NQYFSSLDEIARELIGSLNSNNNPLLKPNDNPKKLIILLASDSGLCGGFNGNTAAFVR
                     DNYENNLENIEAVVVGKKGIHYVKKNKISTLAEYVDLGDTPNVGDASTIVNKAVKEFT
                     DGNFGEVSLVYTKFFSPVKQEVVEEKLLPLDLTGEKGKVSFLIEPDEDEIIDSLVSSY
                     LKGKFMNAMFNSKASEQSARMQAMDGATKNADDLLNSLDAKYNRIRQSIITQEISEIV
                     GGAEAQK"
     gene            5361..6758
                     /gene="atpD"
                     /locus_tag="K8M77_001400"
     CDS             5361..6758
                     /gene="atpD"
                     /locus_tag="K8M77_001400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBI7085900.1"
                     /translation="MSNNIGKVVQVIGPVVDIKFANDELPNIFNAIHIKMDDGKILVC
                     EVEQHVGDDIVRTIAMEATEGLRRGVEAVDTGAPISVPVGECVLGRIFNVLGKPLDSG
                     AEVNNEEKYPIHRPAPSFEEQSVVPQMFETGIKVIDLLAPYQRGGKIGLFGGAGVGKT
                     VLIQELINNIAKEHGGLSVFTGVGERSREGNDLYYEMMESGVIKNTALVFGQMNEPPG
                     ARMRVALTGLTMAEYFRDQGQDVLLFIDNIFRFSQAGSEVSALLGRIPSAVGYQPTLA
                     TEMGALQERITSTTHGSITSVQAVYVPADDLTDPAPATTFNHLDAKTVLSRSIAEIGI
                     YPAVDPLDSSSRILDPRVVGEEHYEVASKVKHILERYKELQDIIAILGVDELADEDKL
                     IVARARRIQKFLSQPFTVAEQFTGMQGRYVPIKETIRGFKEILEGKHDNVPESAFLFA
                     GTIEEVLEKARAMAQ"
     gene            6774..7175
                     /locus_tag="K8M77_001401"
     CDS             6774..7175
                     /locus_tag="K8M77_001401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBI7085901.1"
                     /translation="MNKFKLIVTTPERVLISGEVSRVLCKNAVGEFEILAGHQPYLTA
                     TVPTVTRIDDENGESKYLFTSTGLMKVQNNEVTFCVNSAEWPEEIDEARAMNAKQRAE
                     ERLKNKTDELDKKRAKLALARAMSRLKLKEM"
     gene            7350..7994
                     /locus_tag="K8M77_001402"
     CDS             7350..7994
                     /locus_tag="K8M77_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085902.1"
                     /translation="MNNNIFIAALFCFSLIFSPSTMNLDKKVYSKVEEEICNIEEYGM
                     KIEYITDENIKDQFNNISDYIKIEDFNSIVEKENEIYLNTSKEQFNLYINLIKENSKS
                     RVQMVIVSRNRINFNEIKEYFQVIHNKNMKNIKYYTYIKGNIRENNNIDEIEEKLIKS
                     IGKSKVKEIKRINLEKGTTGIINLKSDYQFNYSIMTYEEDRKLILGTPIIFTTY"
     gene            8017..9282
                     /gene="murA"
                     /locus_tag="K8M77_001403"
     CDS             8017..9282
                     /gene="murA"
                     /locus_tag="K8M77_001403"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBI7085903.1"
                     /translation="MGKIVVQGGKKLKGEVNINTAKNSVLPIIAGSILATDGVLINEL
                     PMLQDVFTICNVIEQLGYDLKIDKKENKLIVPPLNKNPLIPSEYLVKKMRASFLIMGP
                     MIAKYGEFKLARPGGCNIGSRPIELHLKGLRALGAENTNCGNGFVCIKAKKLTGSKIY
                     LDFPSVGATENIMMAATMAKGTTVIENAAQEPEITDLINFLNSMGAKIYIEKPGEIII
                     EGVDSLTSTEYTPIYDRIEAGTFMVAAAITGSEIKINGVNKDHCSAIISKLKEAGTEF
                     FDVHNNENSIIVKGNEEIKPINIKTMPYPGYPTDMQSQMMSLLSIAKGSSIITESVFE
                     NRFMNVDELRRMGANIQIEGRTALIEGVDNLTGCEVKATDLRAGAALILAGLVAKGET
                     IVTDIYHIDRGYVEIENKFRALGADISRI"
     gene            9394..10416
                     /gene="spoIID"
                     /locus_tag="K8M77_001404"
     CDS             9394..10416
                     /gene="spoIID"
                     /locus_tag="K8M77_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBI7085904.1"
                     /translation="MINRKILESVTFIVTIIVSIVFIPILFGFLSSSSMAESNNKVEK
                     VKNKKLVDVVNLKEVEVYISKENKVEKVPLEEYVLSVVSSEMPATFHEEALKAQSILA
                     RTFVINKLITGCNNIKEGNICDTTHCQAYLNVNEKKKAWGKDGDEYLKKIKKVVKETE
                     GKVLSYNDKLVKYPQYFSTSSGNTEDAVAVFSEDVPYLKSVESPGEEISPKYESEISI
                     SISDFKSKIKKSISNSDLGNNITEEVKILNRNTGGTVNDIKIGKATIKGKEFRKIFGL
                     NSANFTLEVLDDNIKIKCLGYGHGVGMSQWGANVMAKEGSKYDEILEHYFKGSKIEES
                     NKILIE"
     gene            10526..11296
                     /locus_tag="K8M77_001405"
     CDS             10526..11296
                     /locus_tag="K8M77_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBI7085905.1"
                     /translation="MNKKDQFKRFIKKDGFYVVLFVCLCLVATVGVVVANKGANNTVE
                     ENIAENTIENTEDNRTTTGNTFDDAELVEGKTDIANDSEEIASNEANGEETKPEANNA
                     DKSVATSSQSQSFTSPIKGGVITRLYNLEPRLNESGQSASVYKGIDIEADEGTEVLAI
                     GDGKVVEAGKGNSKEGCFVKIEHQNGIVGFYGNLDPEIKVKEGDNVKKGDSIGKIGKT
                     IQNSPSDRVSENYLMFHMENSKEPVDPQKYLKDIPVKE"
     gene            11372..11623
                     /gene="spoIIID"
                     /locus_tag="K8M77_001406"
     CDS             11372..11623
                     /gene="spoIIID"
                     /locus_tag="K8M77_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBI7085906.1"
                     /translation="MKDYIEERVLEVAQYIIYSRATIRKTAKVFGVSKSTIHKDMTER
                     LPKINPDIAEEAKSILDLNKAERHIRGGKATKLKYKVVE"
     gene            11714..12751
                     /locus_tag="K8M77_001407"
     CDS             11714..12751
                     /locus_tag="K8M77_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBI7085907.1"
                     /translation="MWFWRKGTDMGIDLGTATVLVYMKDKGIVLKEPSVVAIDKNKNK
                     VLAVGEAARQMIGRTPGNIVALRPMRNGVISDYEMTEAMLKYFIKKGVGNRRISAPKV
                     MICIPCQATGIEKRAVIDAAKNAGAKNVYLIEEPLAAAIGAGLDISKASGHMVVDIGG
                     GTADIAIISLGGIVIRESVKVAGDRFDEAIIKFVRSKYKLMIGERTAEELKINVATVV
                     QGNRKESMEIKGRNLVTGLPGIITVDSEELRIALKDDVSIIGESVHSVLEKTPPELAA
                     DIIEEGILMTGGGALLNGLDKFIEQRTGVKVRVADDAVKCVAEGTGKVLKTFEKLNLG
                     IDDLEVKLIDE"
     gene            complement(12790..13320)
                     /gene="yyaC"
                     /locus_tag="K8M77_001408"
     CDS             complement(12790..13320)
                     /gene="yyaC"
                     /locus_tag="K8M77_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBI7085908.1"
                     /translation="MNKIKIYYKNYLAYYEISNFLKNHIDEKTIIVCIGTDKCIGDCL
                     GPLVGTLLREKFFPLKVFGTLDSPIHALNLDKKITEILKTYPGYKILAIDACLGDSKS
                     IGEIHARNEPIHPGKGVGKSLRSVGDMSIIAIVDSSENIDLFTSRPIRLSFILDMSKV
                     IVDSLIHSYYLKNKKN"
     gene            13408..14058
                     /locus_tag="K8M77_001409"
     CDS             13408..14058
                     /locus_tag="K8M77_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085909.1"
                     /translation="MEVNILMNKGLLSFLVLFFTTLIIGTITYFVNLNYEEAKLKAES
                     VKSQKNEILNINEEENGQKAIENNKEDIEKENISENSNKQSNDLDKSQLKEEKEIEGK
                     SKENATIIKKNEKYKEKVAEEESSKESKEVFKVDKYSIPNKINKADKFKLMSIAKNLS
                     LTDYGVLLKHIKRNDELDAAIDIFKILKDKLEEKEYKEMKDILSPYINIELIEEKI"
BASE COUNT         5770 a         1540 c         2661 g         4252 t
ORIGIN      
        1 aaaaaataaa aaaatgtgaa taaatattaa aaaggttatt caaaaccttt taagttgtat
       61 attttattct taatagtggt ttaaaaattt tttgataaaa accctataaa atatacaatt
      121 ttatgataaa aacttttaaa aaaggatttt aaaatcgaaa atagtaatcg aaatttaatt
      181 taagttctta tatagtaatt taaaaattca gaaaaatgaa atgataaatt taaaaacatt
      241 cataaataat ttgtaaaaga gtaaatgaga gtttttaaga gtaaaaatga aggaaatagt
      301 caaaatcact ttaaaatatc agaatattaa taaattcaac caaaatttaa attttattaa
      361 tattaaattc gggaatataa ttagtaaagt aacattgcgg aactatggag gttatatgtg
      421 aacgctaaaa ttaaaagttt agttttttca attataaaga ctaacatttt attaacaata
      481 atagcaaccc tattaattag tttatttaat tttagatttg gggtggcttt tttagttgga
      541 agtatagttg ctacaattaa tttttcagta aatgcaattg taattcataa agtaataaat
      601 aaaagaagag gtgctctaaa gatacaatta agttttgtat taaggatgat gttaattctt
      661 ttatttacag taatatttat gaaagatgtt aaagtattag cattttactt aataggattt
      721 atttcacatc agatatcaat atttatttat acaaaaaaga ataaggcctt atcaaattaa
      781 taaataaata tttatataca tatataaatt aatactaaga tagaaaggga gtgattccgt
      841 ggatatcttt gcaccaatgt ttacagttaa ggttggtagt ttaacaatta gtagtacaat
      901 tgtttttcaa tgggcagcaa tgctcgttat aacaatattc gctatattgt caactaaaaa
      961 tcttaaaaat aaaccaggta agttacaagt tgttttagag tcactttatc aaacagttta
     1021 taacttggta aagagtaaca tgggggaaag ttatattggt tttgtacctt atataggaac
     1081 attagcagta ttcctacttg tattaaacct ttatggatta atagggcttg cacctcctac
     1141 tcaagattta agtgttgcag tagcattaac aattacttcc tttgttgttg ttcatgttaa
     1201 cgcaatacgt aaatgtcatg ttggaggata tatcaaagga tattttaagc cttatctagt
     1261 aatgttacct ttaaacttaa tggagagaat aattttccca gtatctcttg cactacgttt
     1321 atttggtaat atgttagctg ccactctgtt aatagattta atgtattcag gtctagcaca
     1381 tataggatgg atagctcagt taggtgtgcc aatagttgca catggtttct tcgatgtatt
     1441 cgacggaaca atacaaatga tagtatttac tatgttaaca atggtaaata ttaaaatgac
     1501 agcagaacat tagaaaaatt aaattaacaa atcaaaataa ctttactttg atttaaaaaa
     1561 ggaggaattt ttaatggata tgaagttatt agcagcaggt atagctgtat tagcaggtat
     1621 aggagccgga atcggtatag gaattgcaac agcaggagca atagaagcta ctgcaagaca
     1681 accagaagca tcagataaaa tccaaagttt attcatcatg ggagctggtc tttcagaggc
     1741 aacagctata tatggtttag taatttcaat aatattatta ttcgttgttt aatataaaat
     1801 tgtgtatttt tattaagaac ttatagtaat taataaatga agataataga gttaatataa
     1861 ttgactccga agggaggctg tgattgatat atggaattaa attttatgag aatattagct
     1921 acaatcatta actttataat attaattctt atactaaaac attttttctg ggacaaaatc
     1981 aaaagagcca tcgatgcaag acaagaagct atagatgaaa ctatcttaaa ggccgatgaa
     2041 gatgcagaaa aagcaagaag attaagatta gataatgaaa gaattcttaa atctgctaaa
     2101 gaagatggta gaaagcttag agaagaacag aaaaaagaag ctgatagaat ttataaagaa
     2161 atagttgatg acgctcacag agaagctgag tctataataa atagagctaa tattgaaatt
     2221 caaagagaag aagaaaaagt aaaatatgag ttaaagcaac aagtagttga tatttctgta
     2281 atgctttcag agaaagcact tggagaatct attgatgaat ctaaacatag agagttaata
     2341 aatgatttta ttgaaaaggt aggtatctaa ttatgtatga atatttagat cgtagatatg
     2401 ccttagctct atatgaagtt gcagaagaaa agaataaagt tgaagaatac ttaaatgatt
     2461 taagagaaat ctgtgagatc atttatggaa ataatgaact ttatgaagta ataaaacatc
     2521 ctcaaattag tactgtaaga aagaaaaaga ctttcagaaa catatttgaa ggaaaaatag
     2581 atgatgagtt actttcattt ttaatggttc ttatagaaaa agatagaata ctttatctaa
     2641 gagaaaaatt aaaagaaatg gaaaaaattc atctagaaag aaacaatact ttacttgcag
     2701 aagtaaaaag tgttgttcca ttaacagaag atgaagttac aagacttgta gagaagttag
     2761 aaaataaata ctcaaagaaa attttattaa agcaagaaat agatagaagt atcattggag
     2821 gactttatgt cagagttggc gatgatgtta tagatggtac tgttaaatct agacttgacg
     2881 atatgaaaca gataatgctt aagagagaat agaggtgagt ctatgcatat taaaccagaa
     2941 gaaataactt ctataatcaa gaatgaaata aaaaactatg aaaaagagtt agaaactgtt
     3001 gattctggta ctatcataca gataggtgac ggagttgcca gagtttacgg gttagaagag
     3061 tgtatggaag gtgaacttct tgaattccct aatcaagttt tcggtatggc tcttaactta
     3121 gagcaggata acgttggttg cgttctttta ggatctgaag aaggtataaa agaaggagac
     3181 atcgttaaaa gaactggaaa aatagttgaa gtaccagtag gagaggactt aataggtaga
     3241 gttgttaatt cattaggtca acctatagac ggaaaaggtc ctattaaaaa tgatggatac
     3301 agagctatag aagtaccagc accaggtatc ttagaaagaa gttctgttaa tgagcctcta
     3361 caaacaggta tcaaagcaat tgactcaatg ataccaatag gtagaggaca aagggagtta
     3421 attataggag atagacaaac aggtaaaact gctatagcta tagatactat aattaaccaa
     3481 aaaggtaaag atgttatatg tatatatgtt gctattggac aaaaacaatc aacagtagct
     3541 aacatagtta acactttaac tgaagaagga gctatggact attcaatagt tgtaactgca
     3601 tcagcatcag aatcagctcc attacaatac attgcaccat attcaggatg tacaatgggt
     3661 gaatacttca tgaataaagg taagcatgta ttaataatat atgatgactt aagtaagcac
     3721 gcagttgctt atagaacaat gtcactatta attagaagac caccaggaag agaagcttac
     3781 ccaggggatg tattctacat ccattcaaga ttacttgaaa gagctgctaa gttatcaaaa
     3841 gaaaatggtg gaggctcatt aacagcactt ccaataatag aaacattagc aggagacgta
     3901 acagcatata tcccaactaa cgttatatca ataacagatg gacagatatt cttagaaact
     3961 gagttattta acgcaggaca aagaccagct gttaaccctg gtatatcagt ttcaagggtt
     4021 ggaggtaacg ctcaaataaa agcaatgaaa caagtttctg gtacattaag actagaactt
     4081 gctcaatata gagagttagc atcatttgct caatttggtt cagaccttga taaagatact
     4141 caagcaagac ttgaaaaagg taagagactt atcgagatct taaaacaaga tcaatataag
     4201 ccaatggcag ttgaaaaaca aattatgata atatatgctg cagttaataa tttcttactt
     4261 gatatcaaag tttcagatat taagaaattt gaaaaagaat tcttagaata tatggatact
     4321 catcatagag aaattggtaa agctatatta gataagaaag ttttagacga tgaattaaaa
     4381 tctgctttag aaagtgctat agtagaattt aaaaaaatat tcttaatgta aaataagagc
     4441 atagcttttt aattaaagct atgcccacat ttttaagaat ggaggtggag cttaaatggc
     4501 aggtgcagga cttttagaaa ttaaaagaag aataaaaagt atcaaaaaca caagaaagat
     4561 aacaaaagct atgggacttg ttgccacttc taagttaaga aaagctagac aaaaactaac
     4621 tgaaaacaat caatatttta gtagtctaga tgaaattgct agagaattaa tcggatcatt
     4681 aaatagcaat aataatcctt tacttaaacc taatgataat cctaaaaagt taataatatt
     4741 attagcatca gattcaggat tatgtggtgg atttaatggt aatactgcgg cttttgtaag
     4801 agataactat gaaaataatc ttgaaaacat agaagcagta gtagtaggaa aaaaaggcat
     4861 ccattatgtt aagaaaaaca agatttctac tttagcagaa tatgttgatt taggagatac
     4921 accaaacgtt ggtgatgcgt caactatagt gaataaagct gttaaagaat ttactgatgg
     4981 aaattttgga gaagtaagtt tagtttatac taaattcttt tcgcctgtta agcaagaggt
     5041 tgttgaagaa aaattattac cactagactt aactggagaa aaaggtaaag tttctttctt
     5101 aatcgaacct gatgaggatg aaataataga ttcattagtt tcatcatact taaaaggtaa
     5161 attcatgaat gctatgttta attcaaaagc tagtgaacaa agtgctagaa tgcaggcaat
     5221 ggacggagca actaaaaatg ccgatgattt attaaactca ttagatgcta agtacaatag
     5281 aataagacaa agtatcataa cacaagagat atcagaaatc gttggtggag cagaagcaca
     5341 aaaataagga ggtactcaaa atgtctaata atataggtaa agtagttcag gttattggac
     5401 cagttgtaga tataaaattt gcaaatgatg aacttcctaa tatatttaat gcaatacaca
     5461 taaaaatgga cgatggaaaa atcttagttt gtgaggtaga gcaacacgta ggagacgata
     5521 tagttagaac tatagctatg gaagctactg aaggactaag aagaggtgta gaagctgttg
     5581 atacaggagc acctatatca gtaccagttg gtgaatgcgt attaggaaga atatttaacg
     5641 tattaggtaa accactagat agtggagctg aagttaataa cgaagaaaaa tatccaattc
     5701 atagaccagc tccatcattt gaagaacaat cagttgttcc tcaaatgttt gagacaggaa
     5761 taaaggttat cgacctttta gcaccttacc aaagaggagg aaaaatcggt ctatttggag
     5821 gtgctggtgt tggtaaaaca gttcttatac aagagcttat aaacaacata gctaaagagc
     5881 acggtggact ttctgtattc acaggagttg gagaaagatc aagagaaggt aatgaccttt
     5941 actatgaaat gatggaatca ggagttataa aaaatacagc attagtattt ggacaaatga
     6001 acgaaccgcc tggagcaaga atgagagttg ctttaacagg acttactatg gctgagtact
     6061 tcagagacca agggcaagat gtgttattat tcatagataa catattcaga ttctcacaag
     6121 ctggatcaga ggtttcagct ttattaggaa gaataccatc agctgttggt taccaaccaa
     6181 ctcttgctac agagatggga gctcttcaag agagaatcac atcaactact catggatcaa
     6241 ttacatcagt tcaagcggta tacgtaccag ccgatgactt aactgaccca gctccagcta
     6301 caacattcaa ccaccttgat gctaaaacag ttttatcaag aagtatagct gaaataggta
     6361 tttacccagc tgttgatcct ctagattctt catcaagaat attagaccca agagttgttg
     6421 gagaagaaca ctatgaagtt gcttcaaaag ttaagcatat attagaaaga tataaagaac
     6481 ttcaagatat catagctatc cttggtgttg atgagcttgc agatgaagat aaattaatag
     6541 ttgctagagc aagaagaata caaaaattct tatcacaacc attcacagta gcagagcaat
     6601 ttactggaat gcaaggtaga tatgttccaa taaaagaaac tataagaggt ttcaaagaaa
     6661 tattagaagg taaacatgac aatgttcctg aatcagcttt cttatttgct ggaacaatag
     6721 aagaagtact agaaaaagct agagcaatgg cccaataaaa ggggtgaata aaaatgaata
     6781 aatttaagtt aattgttaca acaccagaaa gagttcttat ttcaggtgaa gtttcaagag
     6841 tcttatgtaa aaatgctgtt ggtgagtttg aaatattagc tggtcaccaa ccttatttaa
     6901 cagcaactgt tcctactgtt acaagaatag atgatgagaa tggtgaaagt aaatacttat
     6961 ttacttcaac aggattaatg aaggttcaaa ataatgaagt tactttttgt gtaaattcag
     7021 cagaatggcc tgaagaaatc gatgaggcaa gagctatgaa tgctaagcaa agagcagagg
     7081 aaagattaaa aaataagact gatgaattag ataaaaagag agctaagtta gccttagcaa
     7141 gagctatgtc tagattaaag ttaaaagaaa tgtaatttat ataaagcata caggaaactg
     7201 tatgcttttt tattattctt attaataaat atttttatgt gattaataga actttttcag
     7261 attattataa tatattgata atataacagg aagtaaatgt ataaaaatac tactcttgac
     7321 aataattata ttggaagggt ggtatattaa tgaataataa tatttttata gcagcattat
     7381 tttgtttctc gcttattttt agtccaagca ctatgaattt agataaaaaa gtttattcta
     7441 aggtagaaga ggaaatttgt aatatagaag agtatggtat gaaaatagaa tatattacag
     7501 atgaaaatat aaaagatcag tttaacaata ttagtgatta tattaaaatt gaagacttta
     7561 atagtatagt agaaaaagaa aatgagatat atttaaatac ttcaaaagag caatttaatt
     7621 tatatattaa tctaataaaa gaaaattcaa agagtagagt tcaaatggtt attgtttcta
     7681 gaaatagaat taattttaat gagattaagg aatattttca agttattcat aataaaaaca
     7741 tgaaaaatat aaaatattat acttatataa aagggaatat tagagagaat aataatatag
     7801 atgaaattga agaaaaactt ataaaatcga ttggtaagag taaggtaaaa gaaatcaaaa
     7861 gaataaatct agaaaaaggc actacaggaa taattaatct taagagtgat taccagttta
     7921 attactccat tatgacatat gaagaagata ggaagttgat tttaggaact cctattatat
     7981 tcacaacata ttaaaataat tttaatggag gataatatgg gtaaaatagt agtacaaggt
     8041 ggaaagaaat taaaaggaga agtaaatata aatacagcta agaattcagt tttaccaata
     8101 attgcaggaa gtatattagc tactgatggt gtactaataa atgaattacc aatgttacaa
     8161 gatgttttta caatttgtaa tgttatagag caattaggat atgatcttaa aatagataag
     8221 aaagaaaaca aattaattgt accaccacta aataaaaatc ctctaattcc aagtgagtat
     8281 ttagtaaaaa aaatgagagc ttcattttta ataatggggc caatgatagc taaatatggg
     8341 gagtttaaat tagctaggcc aggtggatgt aatataggtt ctaggcctat agaattacac
     8401 ctgaaaggac ttagagcgct aggtgctgaa aataccaatt gtggaaatgg atttgtatgt
     8461 ataaaggcaa aaaaattaac aggaagtaaa atatatttag actttccatc agttggagca
     8521 acagagaata taatgatggc agcaactatg gctaaaggga ctacggtaat tgaaaatgca
     8581 gctcaggagc cagaaataac tgatttaatt aattttttaa attctatggg agctaaaatt
     8641 tatattgaaa aaccgggtga aataataata gaaggtgttg attcattaac ttcaactgag
     8701 tacactccta tatatgatag aatagaagct ggaacattta tggttgcagc agcaataaca
     8761 ggatcagaaa taaaaataaa tggagtaaat aaagatcatt gttcagctat aatatctaag
     8821 ttaaaagaag ctggaacaga gttttttgac gtccataata atgaaaatag tataatagta
     8881 aagggtaatg aagaaataaa gccaattaat ataaaaacaa tgccttatcc aggatatcct
     8941 actgatatgc aatcacaaat gatgagttta ttgagcatag caaaaggaag cagtatcata
     9001 actgaaagtg tttttgagaa tagatttatg aatgtagatg aattaagacg tatgggtgca
     9061 aacatacaaa tagaaggaag aacggcttta attgaaggag tagataacct aactggatgt
     9121 gaagttaagg caactgattt aagggcagga gctgctttga ttttggctgg acttgtagca
     9181 aaaggagaga caatagttac tgatatatat catatagata gaggttatgt tgaaatagaa
     9241 aataagttca gagccttagg cgctgacata agtagaatat aagtcaacag tagtactgtt
     9301 gacttttttt actttaaata attttattat aagttcgagt tttagaaaat ttagcatata
     9361 cataaactgt attaataagt tttaggagga tttatgataa atagaaaaat tttagaatca
     9421 gtaactttta ttgtaacaat tatagtgagt atagttttta taccaatact ttttggtttt
     9481 ttatctagtt caagtatggc tgaaagtaat aataaagtag agaaagttaa aaataaaaag
     9541 ttagttgatg tagttaactt aaaggaagtt gaagtttaca tatctaaaga aaataaagtg
     9601 gaaaaagtac cattagagga atatgtctta tctgtagttt caagtgaaat gccagctact
     9661 tttcatgaag aggcattgaa agctcagtct attttagcaa ggacatttgt tataaataaa
     9721 ttaattactg gatgtaacaa tataaaggaa ggaaatatat gtgatactac tcattgtcaa
     9781 gcatatttaa atgttaatga aaagaaaaaa gcttggggta aagatgggga tgaatattta
     9841 aaaaaaataa aaaaagttgt taaagaaaca gaggggaaag ttctatcata taatgataag
     9901 ttagtaaaat atcctcaata tttttctaca agttcaggaa atacagagga tgcagttgct
     9961 gtattttcag aagatgtacc ttacttaaag tcagttgaaa gtccagggga agaaatatca
    10021 cctaaatatg agagtgaaat cagtatttct ataagtgatt ttaagagtaa aattaaaaaa
    10081 tctatttcta atagtgattt aggaaataat ataactgaag aagttaaaat attaaatagg
    10141 aacacaggag gaactgtaaa tgacataaaa atagggaaag ctaccattaa ggggaaagaa
    10201 tttagaaaaa tatttggact aaattctgcc aattttacat tggaagtttt agatgataac
    10261 ataaaaataa aatgtttagg atatggacat ggtgttggta tgagtcaatg gggagctaat
    10321 gtaatggcaa aggaaggcag taaatatgat gaaatattag aacattattt taaaggaagt
    10381 aaaattgaag aaagtaataa aattttaata gagtaataag aagagcaagc aaataaattt
    10441 tgcttgttct ttttttataa aaaagattta tataggtgta ttaatttagg ataaatggac
    10501 agaattagaa acggaggtgt tgaaaatgaa taaaaaagac caatttaaga gatttattaa
    10561 aaaggatggg ttttatgtag ttttatttgt ttgcctatgc ttagtagcaa cagttggagt
    10621 agtggtagca aataaaggtg caaataacac agtagaagaa aatatagcag aaaacactat
    10681 agaaaataca gaggataata gaactacaac tggtaatacc tttgatgatg ctgaacttgt
    10741 tgaaggaaaa acagatatag caaatgactc agaagagatt gcatctaatg aagctaatgg
    10801 tgaagagaca aaaccagaag caaataatgc tgataagtca gttgcaactt caagccaaag
    10861 tcaaagtttt acatcaccaa ttaagggtgg agttataaca agattatata acttagaacc
    10921 tagattaaat gaatcaggtc aaagtgcatc tgtttataaa ggaatagata tagaggctga
    10981 tgaaggaact gaagttttag cgattggtga tggtaaagtt gtagaggctg gaaaaggaaa
    11041 ttcaaaagaa ggttgctttg ttaaaataga gcatcaaaat ggtattgttg gtttttatgg
    11101 aaacttagat ccagagatta aagttaaaga aggggataat gttaaaaaag gagattctat
    11161 aggtaagatt ggaaaaacta ttcaaaatag tccaagtgat agagtttctg aaaattattt
    11221 aatgttccat atggaaaatt ctaaggagcc agtagatcca caaaaatact taaaagatat
    11281 tcctgtaaaa gaataattta atacataatc agctgcttat aaaaaagcag ctgattaaaa
    11341 atttaagaat atttttaagg aggtaaggtt cttgaaagat tatattgaag agagagtttt
    11401 agaagtggct cagtacatta tttattcaag ggctactata agaaaaacag ctaaagtttt
    11461 tggtgtaagc aagagtacta tacataaaga tatgactgaa agattgccta agataaatcc
    11521 tgatatagct gaagaagcaa aaagtatatt agatttaaat aaagctgaaa gacatattag
    11581 aggtggaaag gcaactaagt taaaatataa agttgttgaa tgatgaaaat attccttata
    11641 taataaaagt tagtatttat gtatattttt atagaaatta ataataatta ttatatatta
    11701 gggagttgta aacatgtggt tttggagaaa aggtacagat atggggatag accttggaac
    11761 agcaactgtt cttgtctata tgaaagataa aggtatagtc ttaaaagagc cctcagtagt
    11821 agccatagac aaaaataaga acaaagttct agcagtagga gaagctgcaa gacagatgat
    11881 aggaagaacg ccaggtaata tagtagcatt aagacctatg agaaatggtg ttatatcgga
    11941 ttacgagatg acagaagcta tgttaaagta ttttattaag aagggtgttg gaaatagaag
    12001 aatatctgcc cctaaagtaa tgatatgtat accttgccaa gctactggta ttgagaaaag
    12061 agcagttata gatgctgcaa agaatgcagg agccaaaaac gtatatctta tagaagaacc
    12121 cttagctgct gcgataggag caggattaga tatatcaaag gctagtggac atatggttgt
    12181 agatataggt ggaggaactg cagatatagc tataatatcc ttaggtggaa ttgttataag
    12241 ggagtctgta aaagtagctg gagatagatt tgatgaagct ataattaaat ttgttagaag
    12301 taaatataaa cttatgattg gtgagagaac agcggaggaa ttaaaaataa atgttgccac
    12361 agttgttcaa ggcaatagaa aagaaagtat ggaaataaag ggaagaaatc tagttacagg
    12421 tttaccagga ataataacag tagatagtga agaacttaga attgctttaa aagatgatgt
    12481 tagcataatt ggtgaatcag tacactctgt tcttgaaaaa acaccacctg agctagctgc
    12541 tgacataata gaagaaggga ttcttatgac cggtggaggc gctttattaa atggtttaga
    12601 taagtttatt gaacaaagga caggcgttaa ggttagggtc gctgatgacg ctgtaaaatg
    12661 cgtagctgaa ggaacaggta aagtattgaa aacatttgaa aaattaaatt taggtataga
    12721 tgatttagaa gtaaaattaa ttgatgagta aaattaacta tatatctctt aatattaaga
    12781 gatatatagt taattttttt tgttttttaa atagtaggag tgaattaaag aatctacaat
    12841 aactttagac atatctaata taaagcttaa cctaattggt ctacttgtaa ataggtctat
    12901 attttctgag gaatcaacta tagctattat agacatatct ccaacacttc ttaaggattt
    12961 gccaacgccc tttccagggt gaataggttc atttctagca tgaatttcac cgatagattt
    13021 agaatctcct aggcaagcat ctatggctag tattttataa ccaggataag tttttaatat
    13081 ttcagttatt tttttatcaa gattaagagc atgtatagga gaatcaagtg taccaaatac
    13141 ttttaaagga aagaattttt ctcttaatag ggtaccaact aatggaccta agcaatcacc
    13201 tatacattta tcagttccta tacatactat aatggttttt tcatctatat gattttttaa
    13261 aaaatttgaa atctcgtaat atgccaaata atttttgtag taaattttta ttttattcaa
    13321 ggtttcacct cctcattaat tatatgaagg aagtgttaga aatattaaat ggaagtataa
    13381 aaaagatttt agatggtaat tattaaaatg gaggtgaata tacttatgaa taaagggctt
    13441 ttgagttttt tagtactatt ttttactaca ttaataattg gaacaattac atattttgta
    13501 aacttaaatt atgaagaagc taaattaaaa gcagagagtg taaaaagtca aaagaatgaa
    13561 attttaaata ttaatgaaga ggaaaatgga caaaaggcta tagaaaataa taaagaagat
    13621 atagaaaaag aaaatataag tgagaatagc aataagcaaa gcaatgattt agataaatct
    13681 caattaaaag aagaaaaaga aatagaaggt aagtctaaag aaaatgctac aattatcaaa
    13741 aaaaatgaaa agtataaaga aaaggtagct gaggaggaat catcaaaaga aagcaaagaa
    13801 gtatttaaag tagacaaata ttcaattcct aataagataa ataaagcaga taagtttaaa
    13861 ttaatgtcta ttgctaaaaa tttatcttta actgattatg gagttttatt aaaacatatt
    13921 aagagaaatg atgagttaga tgctgctatt gatatattta aaatacttaa ggataaatta
    13981 gaagagaaag aatataaaga aatgaaagat atattaagtc catacataaa tattgagtta
    14041 attgaagaga aaatataaat gaaaaaagaa gtaaaataaa gaaattgaga gaaatttaaa
    14101 aatagattaa cataatgttt gaaaatagcc taaaattgag tttgaaataa aaagcataac
    14161 aatatatact aagtcttgtc gattggcgat gagccaaaac gaaagagaaa aataataaat
    14221 gtg
//
LOCUS       DAEVGI010000057        13948 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEVGI010000057 DAEVGI010000000
VERSION     DAEVGI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.057"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(390..1766)
                     /locus_tag="K8M77_001410"
     CDS             complement(390..1766)
                     /locus_tag="K8M77_001410"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBI7085910.1"
                     /translation="MKRDFNEFKEFIKGKNVAVVGIGVSNIPLIKFLVKLGAKVTAFD
                     MKSAEELGEISKEFEVLGVNLELGKGYLDRLTGFEVVFKTPSMRIDSEALVRCKNQGA
                     YITSEMEEFVRYCKGKVYGVTGSDGKTTTTTIVSKLLSQEGYKTWTGGNIGTPLFSNI
                     EEIKEEDKVVLELSSFQLMTMDVEIDVAIVTNITPNHLDMHKDMQEYIDAKKNVFKYQ
                     RENDLLVINDENEITKNLDKEAKGKVVRFSSKKTEGEDAYYKDGKLYVHGKEVCKKDD
                     IIIKGMHNVENYLAAFLAVYDEVSIESMKKIAETFGGVHHRCEFIREVDGVKYYNDSI
                     ASTPTRTLAGLKAFEKPVILLAGGYDKHVPFEPLAYEGYEKIKAIVLFGVTKEKIKAA
                     FKRLEEEKGIHVPVYSGESLEEVVSIAKSIAENGDIITLSPACASFDMFKNFEVRGDK
                     FKEIVNNI"
     gene            2077..3120
                     /gene="hydE"
                     /locus_tag="K8M77_001411"
     CDS             2077..3120
                     /gene="hydE"
                     /locus_tag="K8M77_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBI7085911.1"
                     /translation="MNTIIQKAKETHELSRDEIIALLKDDSINEELFKAADEVRKKYL
                     GDEVHLRGLIEFTNICKRNCMYCGLRRDNKSLNRYRLSHEEIIDFAKKAVSYGYKTLV
                     LQGGEDDYYTVERLVPIVKDLKALGVALTLSIGERPFEEYEALKKAGADRFLLRIETT
                     DKELYEKLDPGMSHENRIQCLKNLRKLGYEVGSGCLVGLPGQKIESLADDILFFKELD
                     VDMNGIGPFIPNEDTPLKDAEGGQFELALKVMAIVRLLLPDINIPATTAMETLNKQGR
                     VIALQCGANVVMPNVTEGEYRKLYALYPGKICTGDTPAHCRGCISGKIRGIGRIVSDG
                     PGFRANGFKPKTR"
     gene            complement(3363..4604)
                     /locus_tag="K8M77_001412"
     CDS             complement(3363..4604)
                     /locus_tag="K8M77_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI7085912.1"
                     /translation="MTVNEQNLKEEALRCLMCKNPRCKANCPISTPIPEVIGLFKEDK
                     IKEAGEILFENNPLSVVCSIVCPHENQCKGNCIKGIKDTPVNFSEIEREISEEFLDNL
                     ELTQDTILEDKIAIIGGGPAGITAAFILAKKGYQVTIFDENDKIGGVLRYGIPEFRLS
                     RDIIDKYEEALIKLNVKIRPNTLIGTVITLDMLKSDGYKAIFIATGVWNPKTLNVKGE
                     TLGNVHYAIDYLKAPKSYNLGERVVVIGAGNVAMDAARTAKRNGADTYVLYRKSFEDM
                     PATKVEIEEAKEDGVKFELYKAPLEIYEDGVKYIQTERVISEDGRVSFKTLEGSEGIF
                     KCDSIIVAISQAPRTNIVSSSRDLSTDKYGLVVTDEKGITTLNGVFASGDVVSGPKTV
                     VEAVANSKKVAHAIDEFCKNN"
     gene            complement(4697..5566)
                     /locus_tag="K8M77_001413"
     CDS             complement(4697..5566)
                     /locus_tag="K8M77_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBI7085913.1"
                     /translation="MSMSFLNKGDKIGVISCSNGLNIKHKNIIDELKLNLKSLDIDMV
                     EGDTLYAKEYNLFSGTGEEKARALEKLFLDKDIKMIFDISGGDLANEVLDFLDFNLIK
                     ENPKPFFGYSDLTVLLNAIYSQCHIATYNYQLRNLIDKFKEEQMQNFKASFIEGKEDI
                     FNLDYKWINGSHLEGRVVGGNIRCLLKLAGTKYMPDFKDKILFLESFSGNSAKMVTYI
                     TQYKNLGVFNEVKGIILGEFTEMEREDLKPDIVEILKRVIGEINIPILKTRDLGHGAD
                     AKCIPIGKYLILK"
     gene            complement(5770..7173)
                     /locus_tag="K8M77_001414"
     CDS             complement(5770..7173)
                     /locus_tag="K8M77_001414"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HBI7085914.1"
                     /translation="MAVEKTMDKIVALCKNRGFIFPGSEIYGGLANSWDYGPLGVEFK
                     NNVKRAWWKKFVQESKYNVGLDSAILMNREVWVASGHVGGFSDPLMDCKECKARFRAD
                     KLVEDHMTANGAEVATADGWSNEELMDYITKNNIVCPKCGKLNYTDIRKFNLMFKTFQ
                     GITEDSKNEVYLRPETAQGIFVNFKSVQRTTRKKVPFGIAQIGKSFRNEITPGNFTFR
                     TREFEQMELEFFCKPGTDLEWFNFWKDSCWNFLLNLGMKEENIRMRDHGEEELSFYSN
                     ATSDIEYLFPFGWGELWGIADRTDYDLNKHAEHSGQDMTYLDPTTNEKYVPYVIEPSL
                     GADRVALAFLVEAYDEEELEGANGKVDVRTVMHLHPALAPFKAAILPLSKKLSEKADE
                     VYAELSKYFNVDYDETGSIGKRYRRQDEIGTPFCITVDFDTLEDGCVTVRDRDNMEQQ
                     RVKIEDVRAIIEKSLEF"
     misc_binding    complement(7344..7511)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7632..7706)
                     /locus_tag="K8M77_001415"
     tRNA            complement(7632..7706)
                     /locus_tag="K8M77_001415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7672..7674),aa:Asn,seq:gtt)
     gene            complement(7861..9366)
                     /gene="lysS"
                     /locus_tag="K8M77_001416"
     CDS             complement(7861..9366)
                     /gene="lysS"
                     /locus_tag="K8M77_001416"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI7085915.1"
                     /translation="MSNEEINIHEAEEQLSEQEMLRRQKLVELQEAGKDPFDVYKVER
                     THSSADVKDNFEELEGKEVKVAGRLMSKRGQGKVVFADLADLAGKIQLFIKIDNVGEE
                     ALKEFKTFDLGDWVAATGEVFKTKMGEVSIKVTSFELICKSLKPLPEKWHGLKDPDLR
                     YRQREVDIITNPEVKDTFIKRSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAAKPFI
                     THHNTLDIDMYLRIAPELYLKRLIVAGFEKVYEMGKNFRNEGMSIRHNPEFTTIELYE
                     AYSDYNDMMEITENMVAYVCEKVNGSTKVTYEGTEIDFTPPWRRITMVDAVKEFAGID
                     FNEIKSDEEAQAIAKEKNLEFPKPLDKVTKGEVLNMLFEEYGEDKLIQPTFLVDYPVE
                     ISPLTKKKRGNEMFTERFEGFVYGREVCNAYSELNDPIVQRERFEQQAREREYGDDEA
                     YMLDEEFMSALETGMPPTGGLGIGIDRMIMFLTDSSSIRDVILFPTMKPQK"
     gene            complement(9392..9868)
                     /gene="greA"
                     /locus_tag="K8M77_001417"
     CDS             complement(9392..9868)
                     /gene="greA"
                     /locus_tag="K8M77_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBI7085916.1"
                     /translation="MSEKKHIMTYEGVKKLEDELEYLKTVKRKEITEKIKVALGYGDL
                     SENSEYDEAKNEQAFTEGRIIQLENMLKNAVVVDESEISTDIVTVGSIVKVMDFDFDE
                     EVEYSIVGSAEADPMNFKISNESPVGEGLMGKKVGDVVEIEVPGGTTKFEVLGIRR"
     gene            complement(10147..11127)
                     /gene="dusB"
                     /locus_tag="K8M77_001418"
     CDS             complement(10147..11127)
                     /gene="dusB"
                     /locus_tag="K8M77_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBI7085917.1"
                     /translation="MKISNIVLENNVFLAPMAGVTDISFRGLCKEMGAGIVCTEMVSA
                     KALYYGSENTKELMRIADEEKPVSCQMFGNDPEIMAYVTEKYFNTNDDICIIDVNMGC
                     PAPKIVKNGEGSALMKNPKLAYEIMSSIKKVATKPVTVKFRMGFDEENINAVEFAKIM
                     EEAGVDAIAVHGRTRAQMYEGKANWDIIRQVKEAVNIPVIGNGDVFTAEDALSLKNQT
                     NCDGIMVARGSMGNPWIFKQIVQKLNGEEVYMPTPEEKIDMCIRHYNLSLKYDGEYKA
                     VREMRKHAAWYIKGLNNCTEVKNRINLEADPEKIMGILNEYKEYLMKNDL"
     gene            complement(11134..11913)
                     /locus_tag="K8M77_001419"
     CDS             complement(11134..11913)
                     /locus_tag="K8M77_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBI7085918.1"
                     /translation="MILLIDVGNTNIVLGIHDNEKYIASWRISTDSKKTSDEYSIQVM
                     QLFNQAKLNPEEVEGIIISSVVPNIMHSLENMVRKCFCKEPIVVGPGIKTGINIKYDN
                     PKEVGADRIVNAVAAFEKHKKPMIIIDFGTATTFCAITEKGDYLGGNICPGIQISADA
                     LFERAAKLPRIELEKPKSVICKNTVTSMQAGIIYGYIGKVEYIVKRMKKEMMDLGEKE
                     PFVLATGGLAKLVYSETDVIDEVDRKLTLEGLKILYEKNKE"
     gene            complement(12116..13786)
                     /locus_tag="K8M77_001420"
     CDS             complement(12116..13786)
                     /locus_tag="K8M77_001420"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBI7085919.1"
                     /translation="MKNDIEIAQSAKMEPIINIAKKIGLGEGDIELYGKYKCKISLDA
                     IKRLENNKDGKLVLVTAINPTPAGEGKSTVTVGLGQALNKIGKNTVIALREPSLGPVF
                     GIKGGAAGGGYAQVVPMEDINLHFTGDMHAITSANNLLCAAIDNHIHQGNLLRIDSRR
                     IVFKRVMDMNDRALRNIVVGMGGKINGFLREDGFMITVASEIMAILCMASDLEDLKER
                     MGNILIAYNLDGEPVYAKELEIEGAMALLMKDAIKPNLVQTLENTPAIIHGGPFANIA
                     HGCNSIIATKTALKMSDITITEAGFGADLGAEKFLDIKCRYGNLNPDCIVLVATIRAL
                     KHHGGVKKDELNTSNVDALNKGMKNLEKQIENIKAYGVPVVVAINKFITDSDEEVKAI
                     ENFCKNIGVEVSLTEVWEKGGEGGIDLANKVIKTMEIEPSNFKMIYDSEESIKDKILK
                     IVQTIYGGKGVNYTPQALKQIAEIEKFNLDKLPICMAKTQYSLSDNPSLLGRPENFDI
                     TVKEVRVSNGAGFIVVLTGDVMTMPGLPKVPAANRMDIKDNGEIVGLF"
BASE COUNT         4340 a         2607 c         1487 g         5514 t
ORIGIN      
        1 catcactttg aaactttgtc aacaactttt ttcaaaactt ttaatttgtt ttgttaagtt
       61 gtttctgtgt ctcttagcga cgagatttat cttaccacca tactcattaa cattttatta
      121 taaataacaa gctattacta aaatattctt taaattctta agttttctta tgtttagttt
      181 aaaatccaac tattgatact ccaggttgcg tttttatcta ttttaataag agacatatat
      241 attattgtct tctaaattat tttttataca taaaaacaat ttaaatttta aataaaaata
      301 agccatatta tttttttagc tttaataatt tcatagaatt aattaaacta aaatcataat
      361 atggctcatt ttaagatata tttatctcat tatatattat ttactatttc cttaaactta
      421 tctcctctta cttcaaagtt tttaaacata tcaaaacttg cacaagcagg tgaaagtgtt
      481 attatatctc cattttcagc tattgattta gctatactta ctacctcttc taagctttct
      541 cctgagtata ctggtacatg aattcctttt tcttcttcta atctcttgaa agctgctttt
      601 attttttctt tagttactcc aaataatact atagctttta ttttttcata tccttcataa
      661 gctaaaggct caaaaggtac atgtttatca tatcctcctg ctaaaagtat aactggtttt
      721 tcaaaagctt ttaatccagc taaagttctt gttggagttg atgctataga atcgttataa
      781 tattttactc catcaacttc tcttatgaac tcacatctat gatgaactcc tccaaaagtt
      841 tctgctatct ttttcattga ttctattgat acttcatcat aaacagctaa gaatgcagct
      901 aaataattct ctacattatg cattcccttt atgattatat catctttttt acatacttct
      961 ttaccatgaa catataactt tccatcttta tagtatgcat cttctccttc tgttttcttt
     1021 gaactaaatc taacaacttt tccttttgct tctttatcta aatttttagt tatttcattt
     1081 tcatcattaa ttactaaaag atcattttct ctttgatatt taaaaacatt tttcttagca
     1141 tcaatatatt cttgcatatc tttatgcata tctaagtgat ttggagttat attagtaact
     1201 atcgctacat caatttcaac atccatagtc ataagttgaa aacttgataa ttctaataca
     1261 actttatctt cttcttttat ttcttctatg tttgagaata atggagtacc tatatttcct
     1321 cctgtccaag ttttatatcc ctcttgtgac aataacttag acactattgt tgtagttgtt
     1381 gttttaccat cactaccagt taccccgtaa actttacctt tacaatatct tacgaattcc
     1441 tccatctctg aagtaatata tgcgccttga tttttacatc ttactaaagc ttcactgtct
     1501 attctcatag atggtgtttt aaatacaacc tcaaatccag ttaatctatc taaataacct
     1561 tttccaagtt ctaaatttac tcctaatact tcaaattctt ttgaaatttc gcctaactct
     1621 tcagctgatt tcatatcaaa agcagttact ttagctccta gtttaactaa aaactttata
     1681 agaggtatgt tacttacccc aattcctaca actgctacat tttttccctt tataaattct
     1741 ttaaactcat taaagtctct cttcattttg tggcctccaa attttttacc aatattaata
     1801 atggttcttt ataaattgta tcccatttct ttctaattat atcactatgg aattttccat
     1861 tccatattat tataaattat tcagagttct tctattttgt tctagtgatg attttctaaa
     1921 aatattcatg ttataataaa ttattaatct atttttaact atattgttta aaagagcttg
     1981 ttaaattata attattaatt aataattatt ttaggataaa aagagtaatt tttaatatct
     2041 aaaatcctta tacccaaatt aattaggagg attaatatga atactattat acaaaaagca
     2101 aaagaaactc atgaactttc aagagatgaa attatagcct tattaaaaga tgattctata
     2161 aatgaagaac tttttaaagc tgctgatgaa gttagaaaaa aatatcttgg tgatgaagtg
     2221 catttaagag gacttataga attcactaac atatgtaaaa gaaactgtat gtattgtggt
     2281 cttagaagag ataataaaag tcttaataga tatagacttt ctcacgaaga gattatagat
     2341 tttgcaaaaa aagcagttag ttatggatat aaaactttag ttcttcaagg cggtgaagat
     2401 gattattata ctgttgaacg cttggttcct atagttaaag atcttaaagc cttaggagtt
     2461 gccttaacat taagtatagg tgaaagacct tttgaagaat atgaagcctt aaaaaaagct
     2521 ggtgctgata gattcctttt aagaattgaa acaacagata aagaattata tgaaaaactt
     2581 gatccaggta tgagtcatga aaatagaatt caatgtctta aaaacttaag aaaacttgga
     2641 tatgaagtgg gaagtggatg tcttgttggt cttcctggtc aaaaaataga atccttagct
     2701 gatgatattt tatttttcaa agaacttgat gtagatatga atggtatagg accatttatt
     2761 ccaaatgaag atactccact aaaagatgct gaaggtggac aatttgaatt agccctaaaa
     2821 gtaatggcta tagttagatt acttttacct gatataaata ttcctgctac aacagctatg
     2881 gaaacactaa ataaacaagg aagagtaata gctcttcaat gcggagcaaa tgtagtaatg
     2941 cctaatgtta ctgaaggtga atatagaaaa ctttatgcct tatatccagg caaaatatgt
     3001 acaggcgata cacctgctca ttgtagagga tgtatttctg gaaaaattag aggtataggt
     3061 agaatagttt ctgacggacc tggatttaga gctaatggat ttaaacctaa aactcgttaa
     3121 taaaatataa tataaccaat atttataaaa ctctagaaca atttagtaaa atctaaatta
     3181 gattctagag ttttttattt agagttaaat aataatttct tttcctgcac aagcttaatc
     3241 tcatcaccaa tttaatgctt agctctttta attacataaa aaaactgtag gaaaatttat
     3301 tttatataaa cttcctacag ccttgtctat tatttaattt caatattaaa attaattact
     3361 ttttaattat ttttacaaaa ttcatctata gcatgagcaa cctttttact atttgcaact
     3421 gcctcaacta cagtcttagg cccacttacc acatctcctg aagcaaaaac cccattaagt
     3481 gttgttattc ccttttcgtc agttactact aagccatatt tatctgttga aagatctcta
     3541 ctactagata ctatattagt tctaggagcc tgacttatgg caactattat tgaatcacat
     3601 ttaaatatac cttcactacc ttctaaggtt ttaaatgata ctcttccatc ctcagaaatt
     3661 actctttctg tttgtatgta ttttactcca tcttcataaa tctctaaagg agctttataa
     3721 agttcaaatt ttacgccatc ttccttagcc tcttctattt caaccttagt agctggcata
     3781 tcttcaaagc tttttctata aagaacatat gtatctgcac catttctctt cgcagttcta
     3841 gcagcatcca ttgcaacatt tccagctcct attacaacta ctctctctcc aagattatat
     3901 gattttggtg cctttaaata atctatagca tagtgaacat tacctaaagt ttctccctta
     3961 acatttaaag tcttaggatt ccaaacccca gttgctataa atattgcttt ataaccatca
     4021 ctttttaaca tatctaaagt tataactgtt ccaattaaag tgttaggtct tattttaaca
     4081 tttaatttaa taagggcctc ttcatattta tctattatat ctctagaaag tctaaactct
     4141 ggtattccat atcttaatac tccaccaatt ttatcatttt catcaaatat tgttacttga
     4201 tatccctttt tagctaatat aaatgcagct gttattcccg caggacctcc acctattata
     4261 gcaattttat cttctaaaat agtatcttga gtaagttcta gattatctaa aaactcttct
     4321 gatatttctc tctctatttc actaaagttt actggtgtat cctttattcc ttttatacaa
     4381 ttccccttac attgattttc atgaggacat actatggaac aaactactga taaaggattg
     4441 ttttcaaata aaatttcccc agcctctttt attttatctt ctttaaataa tcctattacc
     4501 tctggaatag gtgtacttat tgggcaatta gccttacatc ttggattttt gcacattaag
     4561 catcttaaag cttcttcttt taagttttgc tcatttactg tcatttttaa taaaccccct
     4621 gatttcttaa caacctataa aaattcttca ttgtttcata atatattata ttatcaaaat
     4681 aatttaataa agtcatttat ttaagaatta aatattttcc tataggtatg catttagcat
     4741 ctgcaccatg tccaaggtct cttgttttta atattggaat attaatttct cctataactc
     4801 tctttaatat ctctactata tcaggcttta aatcctctct ttccatttca gtaaactcgc
     4861 ctaatattat tccttttacc tcattaaaaa cacctaagtt tttatattga gttatataag
     4921 ttaccatctt agctgaattt ccactaaaac tttctaaaaa taatatttta tctttaaaat
     4981 caggcatata ttttgtgcct gctaatttta ataaacatct tatattacca ccaacaactc
     5041 tcccttcaag atgacttcca tttatccatt tataatctaa gttaaatata tcttcttttc
     5101 cttctataaa agaagcctta aaattttgca tttgctcctc tttaaattta tcaattaaat
     5161 ttcttaattg ataattataa gtagctatat gacactgaga atatatagca ttaagtaata
     5221 ctgttaaatc actataacca aagaaaggtt taggattttc ctttattaaa ttaaagtcta
     5281 aaaagtctag aacttcatta gctaaatctc caccagaaat atcaaaaatc atttttatat
     5341 ctttatctaa aaataatttt tctaaagctc tagctttttc ttctcctgtc ccactaaaaa
     5401 gattatattc ttttgcatat agtgtatctc cttcaaccat atctatatct aaacttttta
     5461 aattaagttt taattcatct attatgtttt tatgttttat gttaagtcca tttgaacaac
     5521 ttattacccc tattttatct cctttattta aaaaactcat actcatagca acctcccaaa
     5581 taaaaataat tattatatat aataccactt atttttataa atttatctat tttaaaatat
     5641 tattgattat aattaatttt ttaaatcctc caaaataaat atacacttaa agaaacttat
     5701 aaaaaagagg gtgtatcaaa atgattaaat taatcatttt aatgcaccct ctttattttt
     5761 gcttttaaac tagaattcta agcttttttc tattatagct cttacatctt ctattttaac
     5821 tctttgttgt tccatgttat ctctgtctct tacagttaca catccatctt ctaaagtatc
     5881 gaagtcaact gttatacaga atggagttcc tatttcatct tgtcttctat atctcttacc
     5941 gatacttcct gtttcatcat aatcaacatt gaagtatttg ctaagttctg catatacctc
     6001 atcagctttt tctgataatt tcttagaaag tggtaatata gcagccttaa atggtgctaa
     6061 agctgggtgt aagtgcataa ctgttcttac atctaccttt ccattagctc cttctaattc
     6121 ttcctcatca taagcttcaa ctaagaatgc taatgcaacc ctgtcagctc ctaatgatgg
     6181 ctctataaca tatggtacat atttttcatt tgttgttggg tctaagtaag tcatgtcttg
     6241 acctgagtgt tcagcatgct tatttaagtc atagtctgtt ctatcagcta ttccccaaag
     6301 ttctccccat ccaaatggga ataagtattc tatatctgaa gtagcatttg agtagaagct
     6361 taattcttct tctccatgat ctctcattct tatgttttct tctttcattc ctaagtttaa
     6421 taggaagttc caacaactat ctttccagaa gttgaaccat tctaaatcag ttcctggttt
     6481 acagaagaac tcaagctcca tttgttcgaa ctctctagtt ctgaaagtga agttacctgg
     6541 agttatttca tttctgaaag atttaccaat ttgagctata ccaaatggta ctttctttct
     6601 tgtagttctt tgtactgatt taaagtttac gaatatacct tgagcagttt ctggtcttaa
     6661 atatacttcg tttttactgt cttcagttat tccttggaaa gttttaaaca ttaagttgaa
     6721 ctttcttata tctgtgtagt ttaattttcc acatttagga caaactatat tattcttagt
     6781 tatgtagtcc attaactctt cattgctcca tccatcagct gtagcaactt ctgctccgtt
     6841 tgcagtcata tgatcttcaa ctaatttatc tgctctgaat ctagctttac attctttaca
     6901 atccattaga ggatctgaga atccaccaac gtgacctgat gcaacccaaa cttctctatt
     6961 cattaatata gctgaatcta atccaacatt atatttgctc tcttgaacaa actttttcca
     7021 ccaagctctt tttacgttat ttttgaattc aactcctagt gggccgtaat cccatgagtt
     7081 tgcaagtcca ccgtatattt ctgaacctgg gaatataaat cctctgtttt tacataaggc
     7141 tactatttta tccatagttt tttctactgc cattacaatt acctccattt atattaaata
     7201 acttatttta tattaattct tcactttata aagaacttta aacttccttg agaaatattt
     7261 ctaacagttt tattcactgc tagaaatact catctagatt aacagcctaa gggctttaca
     7321 taaataaaaa aagccattac attcaaaggg acgaaataaa attccgcggt tccacccttt
     7381 ttagcaaaag ctcatctctc aaaataattg tactcaaaag ctcccttcat tttaaacctt
     7441 taataacttt caccaaccgt tatctctcta aaaaagattt taaaactact cttcttcatc
     7501 atagtacata tttagttata ctcttaattt tatcaaaact aaattatatg tcaataaaaa
     7561 aaagaattta taaacataca aaagcttata tatttataaa ttctttctta attaatttta
     7621 gataaaaaaa atggctcctc ggagaggact cgaacctcca acctatcggt taacagccga
     7681 gtgctccacc attgagctac cgaggaacat ttacaaattt attatatcat atttcaaata
     7741 aaatacaata gtttttttaa gtagatttta aaaataatct agaatttttt taggcttttt
     7801 ctcttcaaat aaaataaaaa atagaagtct actttttagt aaacttctac tttatataaa
     7861 ctatttttgt ggtttcattg ttgggaataa tataacgtct cttattgatg aagagtcagt
     7921 taagaacatt atcattctgt ctattcctat tcctaatcca cctgttggag gcataccagt
     7981 ttctaaagca ctcatgaatt cttcatctaa catgtatgct tcatcatctc cgtattctct
     8041 ttctctagct tgttgttcaa atctttctct ttgaactatt ggatcgttaa gctctgagta
     8101 tgcgttacat acttctctac catatacaaa tccttcaaat ctctcagtaa acatttcatt
     8161 tcctcttttc tttttagtaa gaggtgaaat ttctactgga taatctacta agaaagtagg
     8221 ttggattaat ttatcttcac catattcttc aaataacata tttagaactt cacctttagt
     8281 aaccttatct aatggtttag ggaattctaa atttttctct ttagctatag cttgagcttc
     8341 ttcatcactc ttaatttcgt tgaaatctat accagcaaat tctttaactg catcaaccat
     8401 agtaattctt ctccatggtg gtgtgaagtc tatttcagtt ccttcataag taactttagt
     8461 tgatccattt actttttcac aaacataagc aaccatattt tcagtaattt ccatcatatc
     8521 gttataatct gaataagctt catatagttc aatagttgta aattctgggt tgtgtcttat
     8581 agacattcct tcatttctaa agtttttacc catttcataa actttttcaa atccagcaac
     8641 tattaatctc ttaagatata attctggtgc tattcttaaa tacatatcaa tatctaatgt
     8701 attatgatgt gttataaatg gtttagcagc agctccacca gctattggtg aaagaattgg
     8761 tgtatcaacc tctaagaatc ctctattatc taagaattct cttattgctt ttactatttg
     8821 tgatctctta ataaaagtat ctttaacttc tgggtttgtt attatatcaa cttctctttg
     8881 tctgtatctt aagtctggat cttttaatcc atgccatttt tctggtaatg gttttaaaga
     8941 tttacagatt aattcaaatg aagttacttt tattgaaact tcacccattt ttgttttaaa
     9001 tacttctcca gttgcagcaa cccagtcacc taaatcaaaa gttttaaatt cctttaaagc
     9061 ttcctcccca acgttatcaa tcttaataaa taattgaatt tttccagcta agtctgctaa
     9121 atcagcgaat acaactttac cttgacctct ttttgacata agtcttccgg caacttttac
     9181 ttctttacct tctaactctt caaaattatc ttttacatca gcagaagagt gtgttctctc
     9241 taccttatat acatcaaacg ggtccttacc agcttcttgt aattcaacaa gcttttgtct
     9301 tcttaacatc tcttgttcac ttaattgttc ttctgcttcg tgaatattta tttcctcatt
     9361 tgacatttaa aaatccctcc atctatatta tctatcttct tattccaaga acttcgaact
     9421 tagtagttcc tcctggtact tctatctcta caacatctcc aactttttta cccattaacc
     9481 cttcaccaac tggtgattca tttgatatct taaagttcat tggatctgct tctgctgaac
     9541 caactattga atactcaact tcttcatcaa aatcaaagtc cataacctta acgattgatc
     9601 caactgtaac tatatctgtt gatatttctg actcatcaac aacaacagca tttttaagca
     9661 tgttttctaa ttgaattatt cttccttcag tgaaagcttg ctcattttta gcttcatcat
     9721 attctgagtt ctcacttaaa tctccgtatc ctaaagcaac ttttatcttt tcagttattt
     9781 cttttctttt tactgtttta agatattcta attcatcttc taatttttta acaccttcat
     9841 atgtcattat atgtttcttt tcgctcattt tctttctccc ctttgaacat attattataa
     9901 tatcatattt aaacttaacc tgttaatata caattcataa aaactttaag attattaagc
     9961 ctaattattc aaattattat aattcctaaa gattattatg tcaaactata tattatataa
    10021 gatagaatac actaaataaa aaatcattat ttagctttaa ttctcacctt tatttgtata
    10081 aattaaacat ctaatatttt actgatgctt tattatatcc agttatatcc ccatttatga
    10141 taaatattat aaatcatttt tcattaaata ttctttatac tcattaagga tacccataat
    10201 cttctctgga tcagcttcta aattaatcct attcttaact tcagtacaat tatttaatcc
    10261 ttttatatac caagctgcat gttttctcat ttctcttaca gccttatatt ctccatcata
    10321 tttaagtgat aaattatagt gtcttataca catatctatt ttttcttctg gagtaggcat
    10381 ataaacctct tctccattta atttctgaac tatttgcttg aagatccatg ggtttcccat
    10441 acttcctctt gcaaccataa ttccatcaca gttagtttga tttttaagac ttaaagcatc
    10501 ctccgcagta aatacatctc catttcctat aacaggaata ttaaccgctt cctttacttg
    10561 tcttattata tcccagttgg cttttccttc atacatttga gccctagttc ttccatgtac
    10621 agcaatggca tctactcctg cttcttccat gattttagca aactcaacag catttatatt
    10681 ttcttcatca aaacccattc taaacttaac tgttacaggc tttgttgcaa ctttttttat
    10741 gctactcatg atctcataag ctaatttagg atttttcatt aaagctgaac cttcaccatt
    10801 ttttactatc ttaggtgcag gacatcccat attcacatct attatacata tatcatcatt
    10861 tgtattaaaa tacttctcag tgacataagc cattatctct ggatcatttc caaacatctg
    10921 acaagatacc ggtttttctt catctgcaat tctcattaat tcttttgtat tttcactccc
    10981 atagtacaat gcctttgcac taaccatttc agtgcaaaca attcctgcac ccatctcttt
    11041 acataatccc ctaaaagata tatctgtaac ccctgccata ggtgctaaga aaacattatt
    11101 ttctaaaact atgtttgata ttttcattac tttctactcc ttgttttttt cgtaaagaat
    11161 cttaagtcct tctaaagtta atttacgatc tacttcatca attacatccg tttcgctata
    11221 aacaagttta gctagccctc ctgtagctaa tacaaatggt tccttttctc ctaagtccat
    11281 catttccttt ttcatacgtt taactatata ttcaactttt ccaatatatc cataaattat
    11341 tccagcttgc atactggtaa ctgtattttt gcaaattaca ctctttggtt tttcaagttc
    11401 tattcttgga agctttgcag ctctctcaaa taaggcatct gctgaaattt gaattcctgg
    11461 acatatattt ccacctaaat aatctccctt ttcagtaatg gcacagaatg ttgttgctgt
    11521 tccaaaatct attattatca taggtttttt atgcttttca aaagcagcta cagcatttac
    11581 tattctatct gctccaacct ctttaggatt atcatatttt atattaattc cagtttttat
    11641 cccaggtcct actacaatag gttctttgca gaaacatttt ctaaccatgt tttctaaaga
    11701 atgcattata ttaggaacta ctgaagaaat tattattcct tcaacctctt ctggatttaa
    11761 ttttgcttga ttaaatagtt gcataacttg tattgaatat tcatctgatg ttttcttaga
    11821 atctgtagat attctccatg atgctatgta tttttcatta tcatgaatcc ctaaaactat
    11881 atttgtatta cctacatcta ttaataaaat catgtgtttt acaccacctt aatataatta
    11941 aagacagcta ataaagctgt ccggaaaata ccatgtaccc tttatataac aatattaatt
    12001 ttaacaacta ctatacattt gtcaagcaat tacaattact agtttttaaa taaaaaaagg
    12061 cttatatgac tattttttta acagcgtcat ataagcctca aaattatcta aacatttaga
    12121 ataatcctac gatttctcca ttgtctttta tatccattct attagcagca ggaactttag
    12181 gtaaccctgg cattgtcata acatcccctg ttaaaacaac tatgaatcca gctccatttg
    12241 aaactctaac ctctttaaca gttatatcaa agttttcagg tcttcctaaa agacttggat
    12301 tatctgataa tgaatactgt gttttagcca tacatattgg aagtttatct aaattaaatt
    12361 tctcaatttc agctatttgt ttaagagctt gaggagtata gtttactcct tttcccccat
    12421 atatagtttg aactattttt agtattttat cctttattga ttcttctgaa tcgtaaatca
    12481 ttttaaaatt tgaaggctca atttccattg tttttataac tttattagct aaatcaattc
    12541 cgccttctcc acctttttcc caaacttctg ttaagctaac ctctactcct atatttttgc
    12601 agaagttttc tatagcttta acctcttcat ctgaatcagt tataaactta tttatagcaa
    12661 caacaactgg tactccataa gcttttatat tttctatttg cttttctaag tttttcattc
    12721 ctttatttaa agcatctaca tttgatgtat ttaattcatc tttcttcact ccaccatgat
    12781 gttttaaagc tcttatagta gctactaaaa ctatacaatc aggatttaaa tttccgtatc
    12841 tacattttat atctaagaat ttttctgcac ctaaatctgc tccaaagcca gcctctgtta
    12901 tagttatatc actcattttt aaagctgtct tagttgctat tattgaatta catccatgag
    12961 ctatatttgc aaatgggcct ccatgaatta ttgctggagt attttctaaa gtttgaacaa
    13021 ggttaggttt tatggcatct ttcataagta aagccatagc tccttcaatt tctaattcct
    13081 tagcatacac aggctctcca tctaagttat atgcaattaa aatatttccc attctctctt
    13141 ttaagtcttc taaatcacta gccatacata atatagccat tatttctgat gcaacagtga
    13201 tcataaatcc atcttctctt aagaatccgt ttattttacc gcccattcca acaactatat
    13261 ttcttaaagc tctatcattc atatccataa ctcttttaaa tacaattctt cttgaatcta
    13321 ttcttaataa attaccttga tgaatatggt tatctatagc tgcacataaa agattattag
    13381 ctgaagttat tgcatgcata tcccctgtaa agtgaagatt tatatcttcc ataggaacta
    13441 cttgagcata tccaccacca gctgctccac cttttattcc aaatacaggt cctaatgatg
    13501 gctctcttaa ggctataaca gtatttttcc ctattttatt taacgcttgt cctaatccta
    13561 ctgtaacagt agattttccc tctcctgccg gtgttggatt tatagctgta actaaaacaa
    13621 gcttaccatc cttattattt tctagtcttt ttattgcatc taaagatatc ttacatttat
    13681 attttccgta aagctcaata tcaccttctc caagacctat ttttttagca atatttatta
    13741 ttggctccat tttagcactt tgtgctattt caatatcatt tttcataatt ccgcacctca
    13801 ttccaaagta atctacttaa tattataccc ataatttatt aaaaaacaaa gaattttatc
    13861 gaacattttt atatatttta taaaaaatgt tcgtattatt aaaatatctt tagtgaaatt
    13921 ataatataaa ataattctta attaaaca
//
LOCUS       DAEVGI010000058        13907 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEVGI010000058 DAEVGI010000000
VERSION     DAEVGI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 13907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13907
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.058"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..255
                     /locus_tag="K8M77_001421"
     CDS             <1..255
                     /locus_tag="K8M77_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBI7085920.1"
                     /translation="YEVLFIFLSLILSFYLNFSKVFTLFHKIFFNNNYWIFDPKKDPI
                     INVLPESYFLFLAIFILSLVMILSIIFKIFYTLEKHKHLK"
     gene            382..687
                     /locus_tag="K8M77_001422"
     CDS             382..687
                     /locus_tag="K8M77_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI7085921.1"
                     /translation="MNKETHICDLCERKVPRITEHHLIPREKGGRFDETIWLCVPCHK
                     QIHALYSNYELAVRLNSLPRLKDDEKIKRYLKFIKKQPGDANVTIKKSKAIRKKGRY"
     gene            complement(810..1748)
                     /locus_tag="K8M77_001423"
     CDS             complement(810..1748)
                     /locus_tag="K8M77_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HBI7085922.1"
                     /translation="MDFKSVEYGENKVILKDAKNFNIKQVFECGQCFRWERTESGSYI
                     GVAFGKVIELAQEGNDVIIYNTNKEDFENIWIDYFDLERDYSKVKEALSWDETLKSAV
                     EFGYGIRILNQDPFELVISFIISARNSIPVISKTIKKISERWGEPIEYKGNTYYSFPT
                     PESLSKASIDDIRETGASFRSKYIWDTTKNIYNCELAKKGILDAPEEDIVEILEKYDL
                     EKITNMDADGCHKGLQEFMGVGAKVADCIMLFSMKKSSAFPVDVWVKRAMMHFYGADD
                     ASLNKIRIFARERFGEYSGFAQQYLFYYARENGIKI"
     gene            complement(1758..2162)
                     /locus_tag="K8M77_001424"
     CDS             complement(1758..2162)
                     /locus_tag="K8M77_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBI7085923.1"
                     /translation="MLVEKAREKWDKKHDLNCAECIMYAANEEYNLNLSKETLKVMSS
                     FGGGLAIGNVCGAATGAAGVLGLMFTEDRGHQSPQTRALTQEFMEKFYDKLGYYNCTD
                     LKAKYKKDDDRRCIVMIETAAEVLDEIVRRER"
     gene            complement(2405..2905)
                     /locus_tag="K8M77_001425"
     CDS             complement(2405..2905)
                     /locus_tag="K8M77_001425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI7085924.1"
                     /translation="MNSKKIVNTIYYIVISMLVIILVNNFMSKSDSIFKAVGFRTYSI
                     LSGSMEPEINTGDLAIVKSVDAEDVKVGDIITFKYEGKVVTHRVVEKNEEGFITKGDN
                     NNTNDTEIVRGEDLIGKVLFHMPFLGYVTVFLSKPIAISGLMVLIAISILWDTFKVDK
                     KKVIKA"
     gene            complement(3047..4747)
                     /locus_tag="K8M77_001426"
     CDS             complement(3047..4747)
                     /locus_tag="K8M77_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7085925.1"
                     /translation="MLKRFIRYYKPYKKLFILDLLAAFLVSACDLFYPMITRNIINDV
                     IPNKQIKLLFVFAIVLTLIFLIKAGLNYFMQYWGHVVGVRMQADMRRDLFDKLQDMPN
                     KYFDNNKTGVIMSRIINDLLDISELAHHGPEDLFISLVMLVGSFIILCTINVPLTIIT
                     FAIIPFLLFYTIHKRNKMKKAFKETRVKTGEVNATIENSISGVRVTKSFGNKAYEMEK
                     FDKSNDIFKKAREHAYKAMAEYFSGMFFLVDMLELIVLIAAGYFTYLGKINVGDFAAY
                     LLYIKMFLQPIRKLINFNEMFQNGMSGFERYEEIMNEENEKEIPNAKELKDIKGKITI
                     KDVTFRYDNKESILENFNLDIEAGKMVALVGPSGGGKTTICNLIPRFYDYESGQIFID
                     DVDISTVTLKSLRENIGIVQQDVFLFTGTIKENIMYGNPNATDEEVIEAAKNACLHDF
                     IMGLEDGYDTFIGERGVKLSGGQKQRISIARVFLKNPAILILDEATSALDNVTEYEIQ
                     KALEELSKDRTTLVVAHRLSTVKNSDEIVVLTDRGIEERGTHEELIKLGGVYSNLHNL
                     "
     gene            complement(5006..5854)
                     /locus_tag="K8M77_001427"
     CDS             complement(5006..5854)
                     /locus_tag="K8M77_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBI7085926.1"
                     /translation="MEKIFVIGAGTMGAGIVQAFAQKGYEVIVRDIKDEFVDRGIAGI
                     NKGLTKLVSKGKITEEDKEAVLSKITGTTDLGLAADCDLVIEAAVENMEIKKQIFSEL
                     DKICKEETILASNTSSLSITEVASATNRPDRVIGMHFFNPATIMKLVEVIRGMATSQQ
                     TFDKVKTMSEAIGKTPVEVAEAPGFVVNRILIPMINEAIGIYAEGIASVEDIDTSMKL
                     GANHPMGPLALGDLIGLDVCLAIMDVLFKETGDSKYRAHSLLRKYVRAGWLGRKTGRG
                     FYNYNA"
     gene            complement(5968..6975)
                     /locus_tag="K8M77_001428"
     CDS             complement(5968..6975)
                     /locus_tag="K8M77_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI7085927.1"
                     /translation="MNKADFNGVWVFAEQREGQLQKVSLELLGEGRKIADKLGSKLTA
                     LLIGNKVQNLVEDLSRHGADEVLVVDAPELEHYTTDGYTKAICELANAKKPNIIFIGA
                     TFIGRDLGPRVAARLETGLTADCTSLDVDVESGDLLATRPAFGGNLMATIVCPDHRPQ
                     MATVRPGVFEKLPLGENDATVENVEIKFNSNDIRTKIVEIIKEHKDIVDISEANVLVA
                     GGRGIGSEENFKMLKELAEVMNGSIAASRAAVEKGWVDKDYQVGQTGKTVRPNIYVAC
                     GISGAIQHAAGMQDSDMIIAINKDANAPIMKIADYAIVGDVNKVVPEFIAQLKAMKEE
                     A"
     gene            complement(6997..7776)
                     /locus_tag="K8M77_001429"
     CDS             complement(6997..7776)
                     /locus_tag="K8M77_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBI7085928.1"
                     /translation="MKIVVCLKQVPDTTAVKIDPKTGTLIRDGVPSIINPEDKHALEA
                     ALQLKDNNGAEVTVLSMGPPQAKSALREALCMGADKAILITDRAFAGADTLATSKALA
                     GALKKLEYDIIFAGRQAIDGDTAQVGPEIAEHLNIPQVTYVQGVKVEEDGLLVNRALE
                     DGYELIKVEAPVLLTAIKELNEPRYMNVKNIFETSDDEILVWSADDIDVDKAELGLKG
                     SPTKVKRSMTKEAKGAGEIVKESPKDSVTYVLEKLKEKHYI"
     gene            complement(7795..8934)
                     /locus_tag="K8M77_001430"
     CDS             complement(7795..8934)
                     /locus_tag="K8M77_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBI7085929.1"
                     /translation="MNFQLTREQELVKQMVREFAQNEVKPIAAEIDVTERFPMENVKK
                     MAKLGMMGIPFSKEYGGAGGDTLSYILTVEELSKVCATTGVIVSAHTSLCASLIDQFG
                     TPAQKEKYLTPLAKGEKLGAFGLTEPGAGTDAAGQQTTAVLEGDNYILNGSKIFITNG
                     GVADTFIIFAMTDKSQGTRGISAFIVEKNFPGFSIGKLEEKMGIRASSTTELIMENCV
                     VPKENLIGREGKGFGIAMKTLDGGRIGIAAQALGIAEGALEEAVAYMKERKQFGRSLS
                     AFQGLQWMVAEMDTKIEAARFLVYKAACLKEEGKPYSIDAARAKLFAADVAMEVTTKA
                     VQLFGGYGYTKEYPVERMMRDAKITEIYEGTSEVQKMVIAGSILR"
     gene            complement(8981..9763)
                     /locus_tag="K8M77_001431"
     CDS             complement(8981..9763)
                     /locus_tag="K8M77_001431"
                     /EC_number="4.2.1.150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain-enoyl-CoA hydratase"
                     /protein_id="HBI7085930.1"
                     /translation="MELNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIE
                     KQDDIYVVILTGAGDKAFVAGADISEMKDLNEEEGKEFGLLGNKVFRRLENLDKPVIA
                     AINGFALGGGCEISMACDIRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKE
                     LIYTGDMIKADEALRIGLVNKVVEPENLMEEAMALAKKISNNAPIAVKLCKDAIDRGM
                     QVDIDSAVVIEAEDFGKCFATEDQTEGMSAFVERREKNFKNK"
     gene            complement(10022..10660)
                     /locus_tag="K8M77_001432"
     CDS             complement(10022..10660)
                     /locus_tag="K8M77_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBI7085931.1"
                     /translation="MEKKKGISMAVIKRLPKYHRYLQELMENDIDRISSKELSEKIGF
                     TASQIRQDLNCFGDFGQQGYGYNVKELYNNIGNILGLTRDYNTVIIGAGNIGQAIANY
                     NSFNRLGFKLKGIFDANPRMFGIKIRDIEIQDVEKLKDFVRENDIEIGIICVPRTNAQ
                     KVCNDLVEGGIKGIWNFAPIDLDVPKDIRVENVHLSESMMTLVYLLNHNDVK"
     gene            complement(11127..13343)
                     /gene="cadA"
                     /locus_tag="K8M77_001433"
     CDS             complement(11127..13343)
                     /gene="cadA"
                     /locus_tag="K8M77_001433"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBI7085932.1"
                     /translation="MKDIKLYLNGLNCANCAGKIEDKVNKLTDVDEAVLNFSTKLLLI
                     TPKEGVSEKELEEKVEKIVLDLEPDVKVLKHKNKIKYSVNTCNDGCCGEGESHKYNNV
                     ESHSHSHEHGHSHSHGGNSGIEKKELFRLGIALTLFIVGMIVNLDKIYEFIIFGVAYI
                     IAGGEVLLRAFKNILRGQVFDENFLMTIATIGAFAIGEFPEGVAVMLFYEVGEMFQDY
                     AVNKSRKSISDLMNIRPDFANLIDINGEEKRVAPEDVNVGSIIVVRAGEKVPLDGVVL
                     SGEATLDVSALTGESLPKEVEEKDDVLAGSINKSGLLKIKVTKSFGESTVSKILDLVE
                     NAGNKKSPTEKFITKFARYYTPVVVFSALALAIIPPLIISGESFSTWISRALIFLVVS
                     CPCALVVSIPLGYFAGIGLASKNGILIKGSNYLEALNNVESIVFDKTGTLTKGTFKVG
                     KIKSTSELTKEELLKLGAYTEYYSNHPIAKSIENEFNEKINKELISNYEEISGKGIKV
                     DIDGETFLAGNSKLMDMFNIKITSIHEIGTVIYLANEKSELGYIVISDEIKEDSKEAI
                     SGLKENGVKQTIMLTGDNKKVGDNVSKELGLDKAFCNLLPQDKVEKLEEIFQNKSKDK
                     KVAFVGDGINDAPVLARADIGIAMGAVGSDAAIEAADVVIMDDKPSKIIKAIKIAKKT
                     NKIIWQNIIFALFIKIIILIFGALGMANMWEAVFGDVGVTLIAIINSSRILKNKKI"
     gene            complement(13359..13718)
                     /locus_tag="K8M77_001434"
     CDS             complement(13359..13718)
                     /locus_tag="K8M77_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI7085933.1"
                     /translation="MDNNKIESCNCSYIHEDILSRVKEHLPKEEVLYELAEIFKVFGD
                     STRIKILCALFEGEMCVCDMAELLGVSQSAVSHQLRVLKQARLVKFRRDGKIVYYSLA
                     DDHVKHIFNEGLNHVLE"
BASE COUNT         4400 a         2622 c         1411 g         5474 t
ORIGIN      
        1 tatgaagttt tatttatatt tcttagttta atcttaagtt tttacttaaa cttttcaaaa
       61 gtctttacac tatttcataa aatatttttc aataataatt actggatatt tgatcctaaa
      121 aaagatccaa taataaatgt tttgcctgaa tcttattttt tatttttagc aattttcatt
      181 ctatctctag taatgatact ttcaataata tttaaaatat tttatactct agaaaaacat
      241 aaacatttaa aataaaatat attaatttaa aaattcagca taaatcttct taaattttca
      301 aactattaaa gtaacggctt ttttaatttt aaatacatat attataatat ttataagtat
      361 tttatttaag gtgtgataaa tatgaataaa gaaactcata tatgtgatct ttgtgaaaga
      421 aaagttccta gaatcacaga acatcatctc attcctcgtg aaaaaggtgg aagatttgat
      481 gaaacaatct ggctttgtgt tccttgtcat aaacaaatac atgctttata tagtaactat
      541 gaattggctg ttaggcttaa ttctcttcca cgacttaaag atgatgaaaa aattaagcgt
      601 tacttaaaat tcataaaaaa gcaacctggc gatgctaatg ttactataaa aaaatcaaaa
      661 gctattcgta aaaaaggccg ttattaacaa aatgtactat atcaaaataa aacttacaaa
      721 caatataaag agaatatcta aataagcttt agaactttaa tccttagagc ttaatctagt
      781 aatcctctaa atatgtttgt aagtttttat taaatcttta ttccattttc tctagcataa
      841 taaaataaat attgttgagc gaatccagaa tactctccaa atctctctct tgcaaaaatt
      901 cttattttat ttaatgaagc atcatcagct ccataaaaat gcatcattgc tctcttaacc
      961 caaacatcta ctgggaatgc agaacttttt ttcatagaaa atagcattat acaatctgct
     1021 acctttgctc ctactcccat aaattcttga agtcctttat gacatccatc agcatccata
     1081 ttagttatct tttctaaatc atatttttct aatatttcaa ctatatcttc ttctggtgca
     1141 tctagtattc cctttttagc taactcacag ttatatatgt ttttagttgt atcccatata
     1201 tacttgcttc taaatgaagc tccagtctct cttatatcat ctattgaagc cttagataga
     1261 ctttctggag ttgggaatga ataataagta tttcctttat actctattgg ttctccccat
     1321 ctttcactta ttttctttat ggtctttgaa attacaggaa tactatttct tgctgatatt
     1381 ataaatgaaa taacaagttc aaacggatct tgatttaata ttcttattcc atatccaaat
     1441 tcaacagcac tctttaatgt ttcatcccaa gataaagctt cttttacttt tgaataatct
     1501 ctttctaaat cgaaataatc aatccaaata ttttcaaaat cctctttgtt agtattatat
     1561 attattacat cattaccttc ttgagctaac tctataacct ttccaaaggc aactcctatg
     1621 tatgaaccac tctctgttct ttcccatctg aaacattgac cacattcaaa aacttgtttt
     1681 atattaaaat tttttgcatc ttttaatatt actttgttct ctccatattc tacactttta
     1741 aaatccattc ttttcaccta tctttctctt cttacgattt catctaaaac ctcagctgca
     1801 gtctctatca taactatgca tcttctgtca tcatcttttt tgtacttagc ttttaagtca
     1861 gtacaattat agtatcccaa tttatcataa aacttttcca taaattcctg tgttaatgct
     1921 ctagtttgcg gactctgatg acctctatcc tctgtaaaca taaggcctaa aacacctgct
     1981 gccccagttg ctgctccaca tacatttcct attgctaatc ctccaccaaa agaagacatt
     2041 acttttaaag tttcctttga taaatttaaa ttatattctt catttgcagc gtacattatg
     2101 cactctgcac aatttaaatc atgtttctta tcccactttt ctcttgcttt ttcaactaac
     2161 atattatcct cctaaataaa gttttattac tataaacttc tatcttttag ttattataaa
     2221 taaaaacttt atttttaaaa atacatataa ataagattaa acttaaaaca tcaatatttt
     2281 taaataataa ccttatacta ataatattat actaataaaa gttattaata aatagtgttt
     2341 tcatgtaata taaaaagaaa aacatggata gatgttcttt cgaatcaccc tccatgttat
     2401 tgagctaagc ctttattact tttttcttat caactttaaa agtatcccaa agtatgctta
     2461 ttgcgattaa taccataagt cctgatattg ctattggctt tgataaaaat actgtaacat
     2521 atcctaagaa aggcatatga aataaaactt ttcctattaa gtcttctcct ctaactattt
     2581 cagtatcatt agtattatta ttatcaccct ttgttataaa tccctcttca tttttttcaa
     2641 caactctatg ggtaactact ttcccctcat atttaaatgt tattatatct ccaactttaa
     2701 catcctcagc atcaacactc ttaacaatag ctagatctcc agtattaatc tctggttcca
     2761 tacttccact taatattgaa tatgttctaa atccaacagc tttaaatatt gaatctgact
     2821 tactcataaa attatttact aatattataa ctaacataga aataactatg taatatatag
     2881 tattaacaat ctttttacta ttcataaatt catccttgct aaatataaaa tataattctc
     2941 ctattatatt tatattcaag gattatgtaa agtattccta tataaaaata aaaactgtac
     3001 taaaagcaaa taaaattact cttaatacag ttttaattaa actaaattat aagttatgaa
     3061 gattgctata aacaccacca agcttaataa gctcttcatg tgttcctctc tcttctattc
     3121 ctctatctgt taatacaact atctcatcag aatttttaac agttgaaagt ctatgagcaa
     3181 ctacaagggt tgttctatct ttacttaatt cttctaaagc tttttgtatt tcatattctg
     3241 taacattatc taaggctgat gtagcttcat ctaaaattaa tattgctggg ttctttaaga
     3301 atactctagc tattgaaatt ctttgctttt gtcctccaga aagcttaacc cctctttcac
     3361 ctataaatgt atcatatcca tcttctaagc ccataataaa atcatgtaga catgcattct
     3421 tagctgcttc tataacttcc tcatctgtag cattaggatt tccatacatt atattttctt
     3481 ttatagttcc tgtaaataaa aatacatctt gttgaactat cccaatattt tctcttaaag
     3541 attttaaagt aacagtactt atatccacat catcaataaa aatttgacca ctttcatagt
     3601 catagaatct aggtataaga ttacatattg ttgtctttcc tccacctgat ggaccaacta
     3661 aagcaaccat tttccctgct tcaatatcta aattgaaatt ctctaaaata ctctctttat
     3721 tatcatatct aaaagtaaca tcttttattg taatctttcc ttttatatct ttaagttcct
     3781 ttgcattagg aatttctttt tcattttcct cattcattat ctcttcatat ctttcaaaac
     3841 cagacattcc attttggaac atttcattga aatttataag ttttctaata ggctgtaaga
     3901 acatctttat atataataag tatgctgcaa aatctcctac gtttattttt cctaagtaag
     3961 taaaatatcc agctgctatt aaaactataa gttcaagcat atctacaagg aaaaacatcc
     4021 ctgaaaaata ttcagccatt gccttataag catgttctct agctttctta aagatatcat
     4081 tactcttatc aaacttttcc atttcataag ccttattacc aaaagattta gttactctaa
     4141 ctcctgaaat actattttct atagtagcat ttacttcgcc agttttaact ctcgtctctt
     4201 taaatgcttt tttcatttta tttctcttat gaatagtata aaaaagtaaa aaaggtatta
     4261 tagcaaaagt aataattgtt aaaggtacat ttattgtaca taaaattata aatgagccca
     4321 ctaacataac taaggatata aataaatcct ctggaccatg gtgagctaat tctgatatat
     4381 ctagaaggtc atttataatt ctagacataa taactccagt tttattattg tcaaaatact
     4441 tattaggcat atcctgtagc ttatcaaata agtctcttct catatcagct tgcattctaa
     4501 ctccaactac atgtccccaa tattgcataa aatagtttaa gcctgcctta attaagaata
     4561 ttagagtaag tacaattgcg aaaacaaaca ataattttat ctgtttattt ggaataacat
     4621 cattaattat atttcttgta atcataggat aaaataaatc acaagctgaa actaaaaatg
     4681 ctgctaataa atctaaaata aataattttt tatatggttt ataataacgt ataaatcttt
     4741 ttaacatact acctcctttt aatgataaaa attattaatt aacaccaatt ttcattcttt
     4801 taattataat gaactttcta aattaaatca acctgataaa attatttaaa taaaaacctt
     4861 tttacaaaac aaaaagagat tgataaaata tcaatctctt actttgcttc aatctctttc
     4921 tataacatat aacttaaaat atttaataat atcagattta tactatagaa aatttatacc
     4981 ttataaactt tttaaacttt caatattatg cgttgtagtt gtagaatcct ctaccagttt
     5041 ttctacctaa ccatcctgct ctaacatatt ttcttaaaag actgtgagct ctgtatttac
     5101 tgtctccagt ttctttaaat aatacgtcca ttatagctaa gcaaacatct aatcctatta
     5161 aatctcctaa agctaaaggt cccattggat gattagctcc taatttcatt gaagtatcta
     5221 tatcttcaac tgaagctatt ccttctgcat atattccaat agcttcgttt atcataggga
     5281 taagtattct attaactaca aatcctggtg cttcagcaac ttctactgga gtctttccta
     5341 tagcttctga catagttttt actttgtcaa aagtttgttg tgaagtagcc atacctctta
     5401 ttacttcaac aagtttcatt attgttgcag ggttaaagaa atgcattcct ataactctat
     5461 caggtctgtt tgtagctgaa gctacttctg ttattgataa tgaagatgta tttgaagcta
     5521 atatagtttc ttctttacat attttatcta attcagagaa aatttgtttt ttgatttcca
     5581 tattttcaac tgcagcttct ataactaaat cacagtcagc agctaatcct aaatcagtag
     5641 ttccagtgat ttttgataat actgcttctt tatcttcttc agttattttc cctttagaaa
     5701 ctaatttagt aagacctttg ttgattccag ctattcctct atcaacaaac tcgtctttga
     5761 tatctctaac tataacttca tatccttttt gtgcaaatgc ttgaactatt ccagcaccca
     5821 ttgtacctgc accaattaca aaaatctttt ccatattaag ttcctcctaa atactctctt
     5881 taattggact tttctgactt aatagaaaag tcctacacca ttaattgaaa aattttataa
     5941 gtttaccctt gttatattat ttattaatta agcttcttcc ttcatagctt ttaattgagc
     6001 tatgaattct ggaacaactt tatttacatc cccaactatt gcataatcag caatcttcat
     6061 tattggagca ttagcatctt tatttatagc tattatcata tctgaatctt gcataccagc
     6121 ggcatgttga atagctcctg atataccaca agctacatat atatttggtc taacagtttt
     6181 tcctgtttgt ccaacttggt aatctttatc tacccatcct ttttctactg ctgctcttga
     6241 agctgctatt gatccattca taacttctgc taattcctta agcattttaa agttttcttc
     6301 tgaaccaata cctctaccac ctgcaactaa tacgttagct tctgatatat caacaatatc
     6361 tttatgttcc ttaattattt caacaatttt tgttcttata tcattagagt tgaattttat
     6421 ttcaacattt tctacagttg catcattttc tcctagtgga agtttttcaa aaactccagg
     6481 tctcactgta gccatttgtg gtctatgatc tggacatact attgtagcca ttaagttacc
     6541 accaaatgct ggtcttgttg ctaataaatc tccactttca acatcaacat ctaaagatgt
     6601 acagtcagca gttaaaccag tttctaatct tgcagcaact cttggaccta aatctcttcc
     6661 tatgaatgtt gctcctatga aaattatatt tggtttttta gcattagcta attcacatat
     6721 agcttttgta tatccatcag ttgtgtaatg ttctaattct ggagcatcaa caactagtac
     6781 ttcatcagca ccatgtcttg ataaatcttc aactaaattt tgaactttat ttcctattaa
     6841 taatgctgtt aattttgatc ctaatttatc agcgattttt ctaccttcac ctaataattc
     6901 taatgaaact ttttgtaatt gaccttctct ttgctctgca aaaacccaaa cgccattgaa
     6961 atctgcttta ttcatcttaa atacctccca taatccttag atgtagtgtt tttcttttaa
     7021 tttttctagt acataagtaa ctgaatcttt aggtgattct ttaactattt ctcctgcacc
     7081 tttagcttct ttagtcattg atctcttaac ttttgttgga gatcctttta accctaattc
     7141 agctttatct acatctatgt catcagctga ccatactaag atttcatcat cagatgtttc
     7201 aaatatattt ttaacattca tgtatcttgg ctcatttaac tcttttatag cagttaaaag
     7261 tactggagcc tcaactttta ttaattcata tccatcttct aaagctctat ttactaataa
     7321 tccatcttct tcaactttaa cgccttgaac ataagttact tgaggaatat ttaaatgttc
     7381 agctatttct ggaccaacct gtgcagtatc tccatcgata gcttgtcttc ctgcgaatat
     7441 tatgtcatat tctaatttct ttaatgctcc agctaaagcc tttgaagtag ctaatgtatc
     7501 tgcacctgca aatgctctat cagttataag aattgctttg tcagctccca tgcataaagc
     7561 ttctcttaaa gctgatttag cttgaggtgg tcccatactt aatacagtta cttctgctcc
     7621 attattatct tttaattgta atgcagcttc taaagcatgt ttatcctctg ggtttattat
     7681 tgatggaact ccatctctta taagagttcc tgtttttgga tctattttaa cagctgttgt
     7741 atctggcact tgttttaagc aaactactat tttcatctca aagccctcct aaaattatct
     7801 taatatgctt cctgcgataa ccattttttg aacttctgaa gttccttcgt aaatctcagt
     7861 tatcttagca tctctcatca ttctttctac tgggtattcc ttagtatatc catatccacc
     7921 gaataattga actgccttag ttgtaacttc catagctaca tctgcagcga ataatttagc
     7981 tcttgcagca tctatagaat atggtttacc ctcttctttt aagcaagctg ctttatatac
     8041 taaaaatctt gcagcttcta ttttagtatc catttctgct accatccatt gtaatccttg
     8101 gaatgcagat aatgatctac caaattgttt tctttctttc atgtaagcaa cagcttcttc
     8161 taaagctcct tctgctatac ctaatgcttg agcagcgata cctattctac caccgtctaa
     8221 agttttcatt gctattccaa atccttttcc ttctcttccg attaagtttt cttttggaac
     8281 aacacagttt tccatgatta actcagttgt tgaagaagct cttataccca ttttttcttc
     8341 taacttacca attgagaatc ctgggaaatt cttttcaact atgaaagctg aaattcctct
     8401 tgtaccttga ctcttatcag tcatagcaaa gattatgaaa gtatctgcaa ctccaccatt
     8461 agttatgaag attttacttc cgtttaagat ataattatct ccttctaaga cagctgtagt
     8521 ttgttgtcct gcagcatctg tacctgctcc tggttcagtt aatccaaaag cacctaattt
     8581 ttcgcctttt gcaagtggag ttaaatattt ttctttttga gctggagtac caaattgatc
     8641 tattagtgaa gcacataatg aagtatgagc tgaaactata actcctgtag tagcacaaac
     8701 ttttgataat tcttcaacag ttaagatgta agataaagta tctccaccag ctccgccata
     8761 ttctttgcta aaaggaattc ccatcattcc tagtttagcc attttcttaa cattttccat
     8821 aggaaatctt tctgttacat ctatttctgc agctataggc ttaacttcat tttgagcaaa
     8881 ttctctaacc atttgcttaa ctaattcttg ttctcttgtt aattgaaagt tcatttttaa
     8941 ttaccctcct taaatccaca caaccatttt gtttaaaaaa ttacttgttt ttaaagttct
     9001 tttctcttct ctctacaaaa gcactcatac cttctgtttg atcttctgta gcaaaacatt
     9061 ttccaaaatc ttcagcttct ataactactg ctgaatctat atcaacttgc attcctctat
     9121 caattgcatc tttacataac ttaacagcta ttggtgcatt attagatatc ttcttagcta
     9181 atgccattgc ttcttccatt aaattctctg gttcaacaac cttatttact aaaccaattc
     9241 ttaaagcttc atcagctttt atcatgtctc ctgtgtaaat aagttcttta gccttaccag
     9301 gtccaactat tcttggtaat ctttgagttc caccaaatcc tggagttatt cctaatccaa
     9361 cctcaggttg agcaaacttg gcctttgtag ttgctattct tatgtcacaa gccatagaaa
     9421 tttcacatcc accacctaaa gcgaaaccgt ttatagcagc gattacaggc ttgtctaaat
     9481 tttctaatct tctaaacact ttattaccta aaagtccaaa ttctttgccc tcttcttcat
     9541 ttaaatcttt catttctgat atatcagctc ctgcaacaaa tgctttatct ccagcacctg
     9601 ttaaaataac aacatatata tcatcttgtt tttctatatg atctatagca gtatctaagt
     9661 cttttaaagt ttctgagttt aatgcattta atgccttagg tctgttaatt gttaaaaccc
     9721 caatgtttcc ctctttttca aagattacat tatttaattc cattgtattt cctccctcat
     9781 ataccatagc gaatatatgg ttttaaataa cttaacaact aataactaac aacttaatat
     9841 tttatcccat attatcccaa atttattgtt aattttgtaa caaatgaaga taaaaaataa
     9901 ttgaagtact tggtacttca attaaattat aaaacttaaa agtaaatttt tcaatatatt
     9961 tttttgaaaa tctaatacta tttttccata ttaaacaaat ttttataagt caaatccaat
    10021 tttattttac atcattatga tttaaaagat acactaaagt catcatactt tcacttaaat
    10081 gtacattttc tactcttata tcttttggaa catctaaatc tataggtgca aagttccata
    10141 tacctttaat tccaccttca acaaggtcat tacatacctt ttgagcattt gttcttggaa
    10201 cacagattat acctatttca atatcatttt ctcttacgaa atcttttaat ttttcaacat
    10261 cttgtatttc aatatctctt atttttattc caaacattct aggattcgca tcaaaaatac
    10321 cttttaattt aaatcctaat ctgttgaaac tattgtaatt tgcaattgcc tgacctatat
    10381 taccagctcc gattataact gtattatagt ctcttgtaag tcctaatatg tttcctatgt
    10441 tattatataa ctcttttaca ttgtatccat atccctgttg cccaaaatct ccaaaacaat
    10501 taagatcctg tcttatttga gaagccgtaa agcctatttt ttcgcttaac tcttttgaag
    10561 atattctatc aatatcattt tccattaatt cttgtaaata tctatggtat ttaggcaatc
    10621 tttttattac agccatagat atgccttttt tcttttccat tttaatagca cctctttcta
    10681 aaatttttct tttttttaaa aagcaattac tttttagact gtattctaaa ataaaatgat
    10741 aaaattacaa atttaacctt ctaaaatatt accataccct aaaaagtttg tctatatttt
    10801 aacattcttt tggttttact atcaaaaata tatgtattaa agtgtatttt ttacatttaa
    10861 ttgtattttt tatgtcatta attaatatta ttaaatttat ataattctat tataaactat
    10921 ctttttaata aatttctaca aaaaaaataa ggcttgcgtg gctaaaaccc tacgtgcaca
    10981 caagcctatt taaacatttc ataatgttta atataaaagt ttaagggaat tatttacaat
    11041 ctatacccta tgcctgaaag tattcttact atattaagat agcaaaaaat ctagcttttg
    11101 tcaacaacat tagacaaatt tcacgcctat atttttttat tttttaatat cctacttgag
    11161 tttattatag ctattaatgt tacgcctaca tctccaaata cagcttccca catattagcc
    11221 attcctaaag caccaaatat taatattata attttaataa ataaggcaaa tattatattc
    11281 tgccatataa ttttatttgt cttctttgct atttttattg cttttattat cttacttggc
    11341 ttatcatcca ttattaccac atctgccgct tctatagctg catctgatcc aactgccccc
    11401 atggctatac ctatatctgc tctagctaat actggcgcat cattaattcc atctccaaca
    11461 aaggctactt ttttatcttt acttttattt tgaaaaatct cttctagttt ttcaacttta
    11521 tcttgtggta ataaattaca aaacgcttta tctaatccta attctttaga aacattatct
    11581 ccaacctttt tattatctcc agttaacatt attgtttgtt taacgccatt ttcttttaat
    11641 ccacttatag cttctttaga atcctctttt atttcatctg aaataactat atatcctagt
    11701 tcactctttt catttgctaa ataaataaca gtacctattt catgaattga ggtaattttt
    11761 atattaaaca tatccattaa ttttgaatta cctgctaaga atgtttctcc gtcaatgtct
    11821 acttttattc cttttcctga aatttcttca taatttgaaa ttaattcttt gttaattttt
    11881 tcattaaatt cattttctat agatttagca attggatgat ttgaataata ttctgtataa
    11941 gcccctaact ttaataattc ttctttggtt aattcgcttg tgctttttat ttttccaact
    12001 ttaaatgtac cttttgttag tgttccagtt ttatcaaaaa ctatactctc tacattgttt
    12061 aatgcttcta aatagttact tccttttatt aaaattccat ttttagaagc taaaccaatt
    12121 ccagcaaaat atccaagggg gattgataca actaaagcac aaggacatga aacaactaaa
    12181 aatattaaag ctcttgaaat ccatgttgaa aaactttctc cacttattat taaaggtggg
    12241 ataattgcta atgctaaggc agagaataca actactggtg tataatatct tgcaaactta
    12301 gttataaatt tctctgttgg tgatttttta tttcctgcat tctcaactaa atctaatatt
    12361 ttagaaactg tagattctcc aaaacttttt gttaccttta tttttaataa tccactttta
    12421 tttattgatc ctgctaatac atcatctttt tcctcaactt cttttggtaa agattctcct
    12481 gtcaatgcag atacatcaag agttgcttct cctgataaaa ctactccatc tagaggaact
    12541 ttttctcctg ctcttaccac tatgattgat ccaacattaa catcttctgg agctactctt
    12601 ttttcctctc cattaatatc aattaaattt gcaaagtctg gtcttatatt cattaaatca
    12661 gatattgatt ttcttgattt atttacagca taatcttgga acatttctcc tacttcatag
    12721 aaaagcatta ctgctacccc ttctggaaat tctcctatag caaaagctcc aattgttgct
    12781 atagtcatca agaagttttc atcgaatact tgtcctctta aaatattctt aaatgccctt
    12841 aaaagaactt ctccacctgc tataatataa gctacaccaa aaataataaa ttcataaatt
    12901 ttatctaaat taactatcat tcctactata aataaggtta aagctattcc tagtctaaaa
    12961 agttccttct tttctatgcc tgaatttcct ccatgactat gagaatgtcc atgttcatga
    13021 gaatgtgagt gactctctac attattgtat ttatgagact ctccttctcc acaacatcca
    13081 tcattacatg tattaactga atattttatt ttatttttat gctttaaaac tttcacatct
    13141 ggctctaaat ctaaaactat tttttcaact ttctcttcta attctttttc tgaaacacct
    13201 tctttaggag ttataagtaa aagttttgtt gaaaaattaa ggacagcttc atcaacatca
    13261 gtaagtttat taactttatc ttcaattttc ccagcacagt ttgcacagtt taagccattt
    13321 aaataaagtt ttatatcttt catgtctaaa tacctccttt actctaaaac gtgatttaaa
    13381 ccttcattga atatgtgttt aacatgatca tcagctaaag agtaataaac tatcttgcca
    13441 tctcttctaa acttaactaa tctagcttgt tttaaaactc tcaattgatg agatactgct
    13501 gattgactta cacctaataa ttcagccata tcacacacac acatttcacc ttcaaaaagg
    13561 gcacataaaa tttttattct tgttgaatct ccaaaaactt taaatatttc tgctaattca
    13621 taaagtactt cttcctttgg caaatgttct tttaccctac ttaatatatc ttcatggata
    13681 taagaacagt tacaactttc tattttatta ttatccatat tattagctcc tattattcat
    13741 ttttacatat gaacatctgt tcatatgttc acttttatta tatgtctctt ttacaaaaaa
    13801 tgcaactaaa aaagggcgta tatcaaaata actttttata aaacttacaa acattatgaa
    13861 gagaatatct agatgagctt tagaatttaa attattacgt aaaaaat
//
LOCUS       DAEVGI010000059        13731 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEVGI010000059 DAEVGI010000000
VERSION     DAEVGI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.059"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(82..489)
                     /locus_tag="K8M77_001435"
     CDS             complement(82..489)
                     /locus_tag="K8M77_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBI7085934.1"
                     /translation="MNNFFKHTLETHTPQSMSKITSYIREDIKNSNIENGIVVVYCPH
                     TTAGITINENADPDVVKDLIYGFEKVYPTNDHKYRHFEGNSHSHLKSSTVGVSQQLIL
                     HRGRLILGQWQDVYFCDFDGPRNRTFYVKILEG"
     gene            887..1135
                     /locus_tag="K8M77_001436"
     CDS             887..1135
                     /locus_tag="K8M77_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085935.1"
                     /translation="MYTIMAIKISPRTVAAPKVQEILTRYGCIIHTRIGLHEATENTC
                     STSGLILLNLTPSAEEEIKNLDNELNSLEDVTAKLLNL"
     gene            1424..3628
                     /gene="gnpA"
                     /locus_tag="K8M77_001437"
     CDS             1424..3628
                     /gene="gnpA"
                     /locus_tag="K8M77_001437"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HBI7085936.1"
                     /translation="MGELKGRVTIPTDSNMKEKIKSIIDRWGADALRDCDGTKLSKDI
                     VDLGVKLYSTYLTVRSDQKWASENKDELQQLYLMSEPVIAKSEKTEIKILAGYYSEQF
                     EIDKVHDEKKWWEVIDRTTGKVVDPSKWNFNKESEILTVEETTPYHEYTVNFLVYMIW
                     DPTQMYNHITNDWGDKPHEMPYDCRQAKTSVHVKEALKKWIYDNPEVDVVRFTTFFYH
                     FTLCFNEVAKEKFVDWFGYSSSVSPLALEQFEKEKGYKLRPEDIVDAGYYNSTFRVPT
                     KQYKDFIDFQCKHVSKMAKELVDVCHENGKEAMMFLGDNWIGTEPYGKYFLSIGLDAV
                     VGSVGNGTTMRMIADIPGVKYTEGRFLPYFFPDVFNETGDPVGEARENWLQARRAILR
                     SPLDRIGYGGYLSLAVKFPEFVKQVEKICDEFREIHETIEGTKAYIAPFKIAVLNCWG
                     KLRSWQTQQVAHALWYKEIYSYAGIVECLSGMPVEVEFISFDDILENGIPEDIGVIIN
                     AGDAYTAWSGGEYWKNPKLVSMIRKWVHNGGGFIGVGEPTAIHYENKFFQLADVLGVD
                     KEVGFSLSTRKYNELNPHHFITEEIGEKAIDFGEGMKGVYGKGDSYQVLRMDFGNCHC
                     LKNNFRGDTTCAVNTYGKGRSVYFAGFPYTPENCRILLRAIYWAASKEDEMKKFYVTN
                     IDTECAAFLEVGKMAVINNTQEEKITDAYINGVKVETLTLAPMEIRWLNINK"
     gene            3811..5562
                     /locus_tag="K8M77_001438"
     CDS             3811..5562
                     /locus_tag="K8M77_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7085937.1"
                     /translation="MRKIIRKVINTLRGISNFLRKTSIKNRLIASFSVGIIFCSSILG
                     ILLYKKATNALKNNVINYSKNMLSIMSEDIYKNKEFLERASIELSMNEEIANNLINYK
                     FMDGKEKNKFLKYMNDYLSKKLSLLPNLAEVEIISKDYEVTYMQGFKYFKREDIERYA
                     DESENGLFWFSTILNNEPHICIVTSVKQDNSILGYVFMAIKSEVFASSFSLFNIGKGS
                     EIFIIDDNNNIIISENNLNSNFILEETNLNKFLKDKGAKDTARSIVVNGENYVATYRK
                     LKGFNWYIASMIPYDFINADIQSLKKEMTIIGLLFTLVVLIFSYFLYSSIKVPLYNLL
                     NKMKRVSEGDLKVLVNDTYEDEISVISDNFDSMLKRVNELIEKTKLQEVEKRDLEIRM
                     LQAQINPHFLFNTLNSLKWTALMNQDYTVSEGLSSLAELLRNTIIDKNEFVTIREELE
                     NIKNYFVIQKIRYGNSFDVQYEVDELLLENKVIKFILQPIVENSIIHGIDENKENQKI
                     IIKIMSEEENILISIKDNGKGFNSKEKIKDNKLSGIGWNNVDERIKLTFGLDYGTNIY
                     SEVNKGTIVKIVIPKSI"
     gene            5575..7164
                     /locus_tag="K8M77_001439"
     CDS             5575..7164
                     /locus_tag="K8M77_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBI7085938.1"
                     /translation="MFKVIIADDEKIFRIAMRNIIDWESHDFNIVGLAKDGLEVLNIL
                     KEEGADLIITDLKMKGLNGIELIDKLKEENFMGKILVLSNHGEYELVREAMKKGADDY
                     LLKITLRPKELEDIIDKFRESLEEKKKEEEKEAQFKNEFDKTRKIAEISLLKEYLKGD
                     LSYEELLSEFPKINFLDDNCEYYTGGCIYIDNYKKIYEEKIKDKNKLAITIQNIIKEE
                     FSVNGVKIIPINNHKYIFISKIDFNSLKIKTINIQNLIMLYLNIKVSAILIKRIDSVK
                     GLKESFKKVEEILSLKFYGKENFLITSEEFLGFGDAIKLRKELTDAILENIELGEVSA
                     NKELIKEFARRCSKMNVKPSNLINYVNFMCELIENHEYVQDDLNDIKELKGKILNSDN
                     IEELINNSTILIDYINQRYIKSKELSYKREVNEIIKFIEKNIDKRITLVMVANAVNLN
                     ESYLSRIFKSETGKNLMYFINEVKMKKAKELLKQQDTLVKEVANSIGMTDQFYFNRVF
                     KKFYGVSPSKFKSDYNNKVLS"
     gene            7317..8702
                     /locus_tag="K8M77_001440"
     CDS             7317..8702
                     /locus_tag="K8M77_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7085939.1"
                     /translation="MKRKLVKMLTVACVTAIAASAFVGCGNKEETPKDNEKPSTEQAE
                     GSGEKKVLEIAVFEGGFGKDYWEACIEAFEAEHPDVEVKMEANPKIGDIIRPKLSSEK
                     TPDFIYLSTNDPSGIANALIKDKALVDLSDVFDREDPDNPGQKLKDKILPGFLDTPLT
                     TPYGDGKVFLAPLYYNVTGMWYNKALFKEKGWEVPKTWDEFFELGKKAKDEGIALYTY
                     QGQAPGYNEAVIFPMLASAAGEETVEKIFNYEEGAWKDPNVKKALDIFQRMADEDMVL
                     NGTVGMTHTQAQVEFLNGKALFLPCGSWLEGEMKDAIPEGFEFGFMAPPAFKEGDTPY
                     VTTTIEQMYIPAKSDQVELAKEFLAFQYTDAMVKKNAEIAKAVVPVKGAVEKAKSSLD
                     ASGYESYKVVEEGAKPIPLSFKPTNSKLDFRNDSLFGPVGSIINKELTVDEWINNLES
                     DSQTLAKEVVE"
     gene            8781..9707
                     /locus_tag="K8M77_001441"
     CDS             8781..9707
                     /locus_tag="K8M77_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI7085940.1"
                     /translation="MNSNKRKIFLAMCVLPALILFTIFMLYPIGKGFMMSLYEWSGIS
                     GEPVFVGLDNFKALLNDRYFKEAFGNTFFIIVLFPAITITLSLFLAVVITQSKLKEKN
                     FYRVILFFPNILSMVVIGVLFTYVYDPSIGILKAFFEMIGLDKLAGLAWLGDSKLVLW
                     ALVITMVWQAVGYYMVIYIAGLDGISPSLYEVCYLEGASAWQKFRYVTLPLLWDVVRV
                     TFAFFITGALNLSFIFVTVMTKGGPNGKSEVLLTYMYKQAFTNSQYGYAMAVGVTIFA
                     FAMILALIINKLSDSDVREEKRVKRLQQKGVR"
     gene            9709..10590
                     /locus_tag="K8M77_001442"
     CDS             9709..10590
                     /locus_tag="K8M77_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBI7085941.1"
                     /translation="MLETKKKKNVGLDTENKTKKVISNTLLRIILIAATILVIVPIIW
                     TIMSSFKTTKEFYASPWALPKSFGFANYMTAFTEANMGAYFFNSILVTIIAMILSLCL
                     SVPASYVLARQKFFGCSFIKNLFMAGLFIQPVYIIVPLFTILEKMKLLNNLFALALVY
                     AVLSLPFSIYIMSGFMKGITKDYEEAAMIDGCTNLQILTKIVVPLAKPGIITIMIFNF
                     MNYWNEYAMAMTFIIDADKKTLPVGLQNLMEVQKFATDWGALFAGLVIVMVPTMVIYS
                     LVHKKLTEGVNIGGVKG"
     gene            10938..11633
                     /locus_tag="K8M77_001443"
     CDS             10938..11633
                     /locus_tag="K8M77_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI7085942.1"
                     /translation="MKKIVKPWVATLIILIVAFLVYFFVPTANSKINQMIFYLSSMNL
                     DMIKEYILSFGIWAPITSFLLMVLQSVIAPIPAFLITLSNAAIFGWVKGAILSWSSAM
                     AGAALCFYIARGLGRDAVEKLTSKFALKDIDGFFEKYGKHTILIARLLPFISFDLVSY
                     AAGLTSMNFWSFFIATGIGQLPATIVYSYVGGMLTGGAQLLMSGLLILFALSIAIYVG
                     KKVWNEKRGKNDK"
     gene            11623..12735
                     /locus_tag="K8M77_001444"
     CDS             11623..12735
                     /locus_tag="K8M77_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBI7085943.1"
                     /translation="MTNKELKKNLEDCVNCGLCYKVCPMMNTFGDSPKNILRDIDKNK
                     ISYDEIAYSCMLCNACTEACPKDINLKEMFQNLRIKSYRENLKNTSKKFNLKVVQNHQ
                     KGSFSKFLTSKNNHKKIKRVFIPGCSLSGYNNEIIFKIKDYLIENLGEVEILLKCCGK
                     PSKDIGNLELFESNLNDIENNLKIKEVDEIIVACENCYKTFGSAFKDIKVITLWEVIN
                     EFGVPKELKNKYSDLKEEFALHDPCPIKKEEQIHEAVRSILKDLGIKIVEFEKNRSKT
                     ECCGCGGMVGVTNKELWLSQKNKRANSTECENIVSYCESCVNTFKSSDKNALHILDFI
                     FNEDVINGKAKSQKNISTINQWINRIKLANKLGKES"
     gene            12899..13486
                     /locus_tag="K8M77_001445"
     CDS             12899..13486
                     /locus_tag="K8M77_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI7085944.1"
                     /translation="MLDTYGRKYVNPIIDLGANTLLKLRLTPNGVSIIALIIGVLSSV
                     FLYFDKLILSVVFLWISGYLDSVDGAMARKKDLTSPFGTLLDITFDRIVELSIIFVLA
                     LKFQNSRLYLLILTMMILISMTIFLTVGALAQNNGMKSFRYQAGLAERSEGFICFSFM
                     ILLSSIKLNLVTNIFSLIIFITIIQRALEAKKLLK"
     gene            13667..>13731
                     /locus_tag="K8M77_001446"
     CDS             13667..>13731
                     /locus_tag="K8M77_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBI7085945.1"
                     /translation="MAQVKQGRGYVYCIQYHIVWCV"
BASE COUNT         5356 a         1353 c         2458 g         4564 t
ORIGIN      
        1 atttaagtaa attatactaa aaaacagaaa attttggatg aatattattg aaaatataat
       61 taataataaa ttaattaaat attatccctc aagaatctta acataaaaag ttctatttct
      121 tgggccatca aagtcgcaga aatatacatc ttgccattga cctaagatta atctacctct
      181 atgtaatatt aattgctgag atactccaac tgtagaactt ttaaggtggg aatgtgaatt
      241 gccttcaaaa tgtctgtatt tatgatcatt agttggataa actttttcaa atccataaat
      301 taaatcttta acaacatcag ggtctgcatt ttcatttatt gttattccag cagtagtgtg
      361 gggacaataa acaaccacaa ttccattttc aatattacta ttttttatat cctctcttat
      421 ataagatgta attttactca tactttgagg tgtatgagtt tctagtgtat gtttaaaaaa
      481 attattcata aaaacacccc tttaaatctt aatatataaa cattatataa tattttttta
      541 tttgaaataa cataatatta ttaggtttaa tattaataaa tgtggataga atggcatatg
      601 aagaacttat attggaggaa gtaatatgtt tgatttcata tttgataaag aggatagaaa
      661 gattaaggag agaagctttt tagacgagag aaattttata gtgttgttta tttcacagat
      721 ttatttataa aataattacc tatttgaatt tttttaaatg aagtaataat aaaaaatata
      781 gtatattata attatttttg taaataataa ttaaagatta tacaagaaaa atttactaat
      841 atatgctaat atgtaattag gattattatt tatggaggga tttattatgt acactataat
      901 ggctatcaaa atatcaccta ggactgttgc agcaccaaaa gttcaagaaa tactaactag
      961 atatggttgt ataatacata ctagaatagg attacacgaa gctactgaaa atacatgttc
     1021 aacttcaggt cttattcttt taaaccttac accaagtgct gaggaagaaa taaaaaattt
     1081 agataatgaa ttaaattctt tagaagatgt aacagctaaa ttattaaacc tttaataagc
     1141 tcgtaaaaag tcgtatatga aaataatttt taaaaaactt acaaatagta ttaaaagaac
     1201 ttttagtggt tcttataata tgtttgtaag ttttttattt taattaagga cttttaatat
     1261 taaattatta ttaaaaataa tatactcaaa attttcaatg atttagtaaa taaattacat
     1321 gtaaacgtta attaacaaat gttaatatta aattgtaaga aaaaggttat attaatatac
     1381 atgactttaa atgtttatag caggggaaat ggaggagaaa tttatgggtg agttaaaggg
     1441 aagagttaca ataccaactg attctaatat gaaagagaaa ataaaaagta ttatagatag
     1501 atggggagca gatgctttaa gagattgtga tggcactaag ctttctaagg atatagttga
     1561 cttaggggtt aagttatatt ctacatacct aacagttaga agtgatcaaa aatgggctag
     1621 tgaaaataaa gatgaattac aacaacttta cttaatgtca gagcctgtta ttgctaaatc
     1681 agaaaaaact gaaataaaaa tattagccgg atattattct gagcaatttg aaatagataa
     1741 ggttcatgat gaaaagaaat ggtgggaagt tatagataga actactggta aagttgtaga
     1801 tccttcaaaa tggaatttta ataaagagag tgaaatctta acagtagaag aaactacacc
     1861 ttatcatgaa tatacagtaa acttcttagt ttatatgatt tgggatccaa ctcaaatgta
     1921 taatcatata actaatgatt ggggagataa acctcatgaa atgccatatg actgtagaca
     1981 agctaagact agtgtacatg ttaaagaagc tttaaaaaaa tggatttatg ataatccaga
     2041 agtagatgta gttagattta ctactttctt ctatcacttt acattatgct ttaatgaggt
     2101 tgctaaggag aaatttgttg attggtttgg atatagttca tcagtgagtc ctttagcttt
     2161 agaacaattc gaaaaagaaa aaggatataa attaagacca gaagatatag ttgatgctgg
     2221 ttattataat tcaacattca gagtgccaac aaaacaatat aaagatttca tagattttca
     2281 atgtaagcat gtttcaaaaa tggctaaaga gttagtggat gtatgtcatg aaaatggaaa
     2341 agaagcaatg atgtttttag gggataactg gataggtaca gagccttatg gaaagtattt
     2401 cctaagcatt ggattagatg cagtggttgg ttcagtaggt aatggaacaa ctatgagaat
     2461 gatagctgat attccaggag ttaaatatac agaaggaaga tttttaccat attttttccc
     2521 agatgtattt aatgaaacag gagaccctgt tggggaagca agggagaatt ggttacaagc
     2581 aagaagagct attttaagaa gtccactaga tagaatagga tatggtggat atttaagcct
     2641 tgcagttaaa ttcccagagt ttgttaagca agttgaaaaa atatgtgatg agtttagaga
     2701 aattcatgaa actatagaag gaactaaggc gtatatagcc ccatttaaga ttgcagtatt
     2761 aaactgttgg ggtaaattaa gaagctggca aactcaacaa gtagctcatg ctctttggta
     2821 taaggaaata tattcatatg ctggaattgt tgaatgttta agtggtatgc ccgtagaagt
     2881 agaatttatt agctttgatg atatattaga aaatggaatt ccagaggata ttggagttat
     2941 tataaatgct ggagatgctt acactgcttg gagtggtggg gaatactgga aaaatcctaa
     3001 attagtttct atgattagaa aatgggttca taatggtggg ggattcatag gagttggaga
     3061 accaactgca atacattatg aaaataaatt cttccagtta gcagatgttt taggagttga
     3121 caaggaagtt ggatttagtt taagcactag aaaatataat gaattaaatc cacatcattt
     3181 tataacagaa gaaattggtg aaaaagctat tgattttggt gaggggatga aaggcgttta
     3241 tggaaagggt gatagttacc aagttttaag aatggacttt ggtaattgcc attgcttaaa
     3301 gaataacttc agaggagata ctacttgtgc tgtaaacaca tatggaaaag gtagaagcgt
     3361 ttattttgca ggatttccat atactccaga gaattgtaga attttattaa gagcaattta
     3421 ttgggctgca agtaaagaag atgagatgaa aaaattctat gtaactaata tagatactga
     3481 gtgtgctgcc ttcttagaag taggaaaaat ggctgtaata aacaacactc aagaagaaaa
     3541 aattacagat gcatatataa atggtgttaa ggttgaaact ttaaccttag ctcctatgga
     3601 aataagatgg ttaaatataa ataaataaat aagttttttc ttttaattcc cttatataga
     3661 tccttagtga gtttttcact aagggtcttt ttatatttta tataattttt gtaaaattta
     3721 gtaaaacaat aaagttagag tgggatgtta gtttcaaaaa aataccatat atgataaaat
     3781 attaaaaaag tatagaaatt atattaagga gtgagaaaaa taataaggaa agttataaat
     3841 accttaagag gaatatcaaa ttttcttaga aaaacttcta ttaaaaatag acttatagca
     3901 tcattttctg taggaataat tttttgtagt agtattttag gcatactcct atataaaaag
     3961 gctaccaatg cacttaagaa taatgtaata aattattcga aaaatatgct ttctatcatg
     4021 agtgaagata tttataaaaa taaagaattt ttagaacgag catccattga attatcaatg
     4081 aatgaagaaa tagctaataa tcttataaac tacaaattta tggatggaaa agaaaagaat
     4141 aaatttttaa aatatatgaa tgattattta tctaaaaaac tttctctttt acctaatcta
     4201 gcagaggttg aaataatatc aaaggattat gaagttactt atatgcaagg ctttaaatat
     4261 tttaagagag aagatataga aagatatgca gatgaatctg aaaatggact attttggttt
     4321 tctacaattt taaataatga acctcacata tgtatagtaa cttcagtaaa acaggataac
     4381 agtattttgg gatatgtttt tatggctata aaaagtgaag tttttgcctc ttctttttct
     4441 ctttttaata ttggtaaagg tagtgaaatt tttataatag atgataataa taacataata
     4501 atttctgaaa ataatttaaa tagtaatttt atattagagg agacaaattt aaataagttc
     4561 cttaaagata aaggggctaa ggatacagca aggagtatag tggtaaatgg agaaaattat
     4621 gtagctacat atagaaaatt aaagggattt aattggtata tagcttcaat gattccttat
     4681 gattttataa atgctgatat tcaaagctta aaaaaagaga tgactataat aggattactt
     4741 tttactttag tagtattaat attttcatat tttttatatt caagtattaa agtaccttta
     4801 tataaccttt taaataagat gaaaagagta tctgaggggg atttgaaggt tttggtaaat
     4861 gatacttatg aagatgagat tagtgttatt tctgataatt ttgattctat gttaaaaaga
     4921 gtaaatgaat taattgaaaa gactaaatta caagaagttg agaaaaggga tttagagata
     4981 aggatgttac aggcacaaat taatcctcat tttcttttta atacattgaa ctctttaaag
     5041 tggacagcct taatgaatca agattataca gtaagcgagg ggcttagttc actagcagaa
     5101 ttattaagaa atactatcat agataaaaat gaatttgtaa ctattagaga agaactagag
     5161 aatataaaaa attattttgt aattcaaaag ataagatacg gtaattcttt tgatgttcag
     5221 tatgaagtag atgaactttt attagaaaat aaagttataa agtttatttt acaaccaata
     5281 gtagaaaatt caataattca tggaattgac gaaaataagg aaaatcaaaa gattattatt
     5341 aaaattatgt cagaggaaga aaatatttta atatcgatta aggataacgg aaaggggttt
     5401 aatagtaaag aaaagattaa agataataaa ctttcaggta ttggatggaa taatgttgat
     5461 gagagaataa aattaacatt tggattggat tatgggacaa atatttatag tgaggtgaat
     5521 aaaggaacta ttgttaaaat agtaattcct aagagcatat agggggaaat ttttatgttt
     5581 aaggttatta ttgctgatga tgaaaaaata tttagaattg ctatgagaaa tataatagat
     5641 tgggaatctc atgattttaa tatagttgga ttagccaaag atggtttaga agttcttaat
     5701 atactaaaag aagagggggc agatcttatt ataacagatc ttaaaatgaa aggattaaat
     5761 gggattgaat taatagataa gcttaaagaa gagaatttta tggggaagat acttgttctt
     5821 agtaatcatg gggagtatga attagttagg gaagcaatga aaaagggtgc agatgattat
     5881 ttattaaaaa taactttaag accaaaggaa ctagaagata taattgataa atttagggag
     5941 agtttagaag agaagaaaaa ggaagaagag aaagaagctc agtttaaaaa tgaatttgat
     6001 aaaacaagaa aaatagctga gatttcttta ttaaaggaat atttaaaggg tgatctatct
     6061 tatgaagaac ttttaagtga atttccaaag attaattttt tagatgataa ttgtgaatat
     6121 tatactggtg gctgtattta tatagataat tataaaaaga tttatgaaga aaaaattaag
     6181 gataaaaata aattggcaat tactatacaa aacataataa aagaagaatt tagtgtaaat
     6241 ggggttaaga ttatcccaat aaataatcat aaatatattt ttatatctaa aattgatttt
     6301 aatagtttga agataaaaac aataaatata caaaatttaa taatgcttta tttaaatatt
     6361 aaagttagtg ctattttaat aaaaagaata gatagtgtga aaggcttaaa agagagtttt
     6421 aaaaaggttg aggaaattct tagtctaaag ttttatggaa aagaaaattt tttaataaca
     6481 agtgaagaat ttttggggtt tggtgatgct attaagctta gaaaggaatt aacagatgcc
     6541 attttagaga atatagagtt aggagaagtt tctgctaata aggaacttat aaaagaattt
     6601 gctagaaggt gttctaagat gaatgtaaaa ccttcaaatt tgataaacta tgtaaatttt
     6661 atgtgtgaat taatagagaa tcatgaatat gtacaagatg atttaaatga tataaaagaa
     6721 ttaaaaggaa agatattaaa tagtgataac atagaggaat taataaataa ttcaactatt
     6781 ttaatagatt acatcaatca aagatatata aagagcaaag agcttagtta taaaagagag
     6841 gttaatgaga ttataaagtt tatagaaaag aatattgata agagaataac cttggttatg
     6901 gtggctaatg ctgtgaattt aaatgaaagt tatttaagta gaatttttaa aagtgaaaca
     6961 ggaaaaaatc ttatgtactt tataaatgaa gtaaagatga aaaaggctaa ggaattatta
     7021 aaacagcaag atactttagt taaagaggtt gctaacagta ttggaatgac agatcagttt
     7081 tattttaata gagtttttaa gaagttttat ggggtaagtc catctaagtt taaaagtgat
     7141 tataataata aagttttgtc ataaatatag attattttta ttttggattg atattgtaaa
     7201 aaaagtacat gtttaagtaa aatccttata ttcaaaaaaa aaccaataag aagtattata
     7261 aatattgcaa gaaaatgttt acaagaaatg agtaagggta ttggaggaga gagtttatga
     7321 aaagaaaatt agttaaaatg ctaactgttg catgtgttac agctatagct gcctctgcat
     7381 ttgttggatg tggaaataaa gaagagacac caaaagataa tgaaaaacca tcaactgaac
     7441 aagctgaagg ttcaggagag aaaaaggttt tagaaatagc tgtttttgaa ggtggatttg
     7501 gtaaggatta ctgggaagct tgcatagagg cttttgaagc tgaacatcct gatgtagaag
     7561 ttaagatgga agctaatcct aaaataggtg atattataag gcctaaatta tcatcagaaa
     7621 aaacacctga ttttatatat ttaagtacaa atgatccatc aggtatagct aatgctttaa
     7681 taaaggataa agctttagta gatttaagtg atgtattcga tagagaagat ccagataatc
     7741 caggacaaaa attaaaagat aaaatattac caggattttt agatacacca cttacaactc
     7801 catatggaga tggaaaagta tttttagcac cactttacta taatgttaca ggtatgtggt
     7861 ataacaaagc attattcaaa gaaaaaggat gggaagttcc aaaaacttgg gatgagttct
     7921 ttgaattagg taagaaggca aaagatgagg gaatagctct ttatacttat caaggacaag
     7981 cgccaggata caatgaggct gtaatattcc caatgttagc tagtgcagct ggagaagaaa
     8041 ctgtagaaaa gatattcaac tatgaagaag gtgcatggaa agatccaaat gttaaaaaag
     8101 cattagatat attccaaagg atggctgacg aggacatggt tcttaatgga actgttggta
     8161 tgactcatac tcaagcacaa gttgaattct taaatggaaa agcattattc ttaccatgtg
     8221 gtagctggtt agaaggagaa atgaaggatg ctataccaga aggatttgag tttggattta
     8281 tggctccacc agcatttaaa gaaggggata ctccatatgt aactactaca atagagcaaa
     8341 tgtatatacc agctaaatca gatcaagttg aattagcaaa agaattctta gcattccaat
     8401 atacagatgc tatggttaaa aagaatgctg aaatagctaa ggctgtagtt ccagttaagg
     8461 gagcagttga aaaagctaaa tcttcattag acgcatcagg atatgagtct tataaggttg
     8521 ttgaagaagg tgctaaacca attccacttt catttaaacc aacaaactct aaattagatt
     8581 ttagaaatga tagtttattt ggaccagtag gaagtatcat aaataaagaa ttaacagttg
     8641 atgaatggat taataactta gaatctgatt cacaaactct tgctaaagaa gttgttgaat
     8701 aattattaag ataaaataaa atttattgag aaggagagga gattcttctc cttctttaaa
     8761 attatagggg gtgaaatttc atgaacagta ataaaagaaa aatattttta gcaatgtgtg
     8821 tgttgcctgc acttatacta tttacaatat ttatgttata cccaattgga aaaggtttca
     8881 tgatgtcttt atatgaatgg agtgggattt caggagaacc agtatttgtg gggttagata
     8941 actttaaagc acttttaaac gatagatatt ttaaggaagc atttgggaat acttttttca
     9001 taatagtttt atttccagca ataacaataa ccctttcttt attcttagct gtagtaataa
     9061 ctcaaagtaa gttaaaagaa aagaatttct acagagttat actatttttc ccaaatatat
     9121 tatcaatggt tgttataggg gttttattta catatgttta tgacccatca ataggaattc
     9181 ttaaagcatt ttttgaaatg ataggactag ataagttagc aggtctggca tggcttggag
     9241 actctaagtt agtcctatgg gcattagtta taactatggt atggcaagca gttggttatt
     9301 atatggtaat ttatatagct ggcttagatg gaatatcacc ttctctttat gaagtatgtt
     9361 atttagaagg ggcttcagct tggcaaaagt ttagatatgt aaccttacca cttttatggg
     9421 atgtagttag agtaaccttt gcattcttta ttacaggtgc attaaatcta agctttatct
     9481 ttgtaactgt aatgactaag ggtggaccaa atggaaaatc agaggtttta ttgacatata
     9541 tgtataagca agcatttaca aattctcaat atggatatgc tatggcagtt ggggtaacta
     9601 tatttgcctt tgctatgatc cttgcattaa taatcaataa gttaagtgat tctgatgtaa
     9661 gggaagaaaa gagagtaaaa aggttacaac agaagggagt gagataacat gttagaaact
     9721 aaaaagaaaa aaaatgtagg tttagatact gaaaacaaaa ctaaaaaagt aatatctaat
     9781 acattattaa ggattatatt aatagctgca actattttag ttatagtgcc aattatatgg
     9841 acaataatgt cttcttttaa aactactaaa gaattctatg caagtccatg ggcattacca
     9901 aaaagttttg ggtttgctaa ctatatgact gcatttacag aagctaacat gggagcttac
     9961 ttcttcaact caatattagt tactataata gctatgattt tatctctatg cttaagtgta
    10021 ccagcatctt atgttttagc tagacaaaaa ttctttggat gtagttttat aaagaattta
    10081 tttatggcag gattatttat ccaaccagta tatattatag tacctttatt tacaatatta
    10141 gaaaaaatga aattgttaaa taatttattt gcactagctc ttgtatatgc agtgctaagc
    10201 ttacctttct caatctatat aatgagtgga tttatgaagg gaattacaaa ggattatgaa
    10261 gaggcagcaa tgatagatgg atgtactaat cttcaaatat taacaaagat agtagttcca
    10321 ttagctaagc caggtatcat aactataatg atattcaact ttatgaatta ttggaatgaa
    10381 tatgctatgg caatgacttt cataatagat gcagataaaa agacattacc agtagggtta
    10441 caaaacttaa tggaagttca aaagtttgca actgactggg gagcattatt tgcaggatta
    10501 gttatagtaa tggtacctac aatggttata tactcactag ttcataaaaa gttaacagaa
    10561 ggtgttaaca ttggtggagt taagggctaa atttattagc atttagaaaa gaaaatagta
    10621 ttattaaaat aagagcagat gtcactttta gtgaggtctg cttttttagt taaaataaaa
    10681 ttaaaattta aatttacaat aatcagtata gttatttaga acttttgcta tataattaat
    10741 tagtgacttt aaactaagaa tttaaggttt gccaattaga cttaaaggtt aataaaaaca
    10801 ttattattaa tttaagctaa tctttattta aaaggttaac tttaaaataa aggcttaaag
    10861 ttttttagat taaccttatt aaaaagtaga ggtttatttt ataataatga aatattgatt
    10921 taaatggagg taataatttg aagaaaatag ttaaaccttg ggttgctacc ttaattattt
    10981 taatagtagc ttttttggtg tatttttttg ttccaactgc taatagtaaa ataaatcaga
    11041 tgatatttta cttaagtagt atgaatttag atatgataaa ggaatatata ttatcttttg
    11101 gaatatgggc acctataaca tcatttttat taatggtact tcaatctgta atagcaccaa
    11161 taccagcttt tttaataacc ttaagtaatg cagctatatt tggatgggtt aagggtgcta
    11221 tattatcatg gagtagtgcc atggctgggg cagcactatg tttttatata gcaagaggct
    11281 taggaagaga tgcagttgaa aaattaacca gtaaatttgc cttaaaggat atagatggtt
    11341 tctttgaaaa gtatggtaaa catactatac ttatagcaag attactacct tttatatctt
    11401 ttgacttagt tagttatgct gcaggactta catcaatgaa tttttggtca tttttcattg
    11461 ctacaggtat tggacaatta cctgctacaa tagtatattc ttatgttgga ggtatgctta
    11521 ctgggggagc acaattactt atgagtggac ttttaatttt atttgcttta agtatagcaa
    11581 tatatgtagg gaaaaaagtt tggaatgaga aaagaggtaa aaatgacaaa taaggaacta
    11641 aagaaaaatt tggaggattg tgttaattgt ggactttgct acaaggtttg tccaatgatg
    11701 aatacttttg gagatagtcc taaaaatata ttaagagata tagataagaa taaaataagt
    11761 tatgatgaaa ttgcatattc ttgtatgctt tgtaatgcat gtactgaagc atgtccaaaa
    11821 gatataaatt taaaagagat gtttcaaaat ttaagaataa aatcttatag agagaattta
    11881 aaaaatacta gcaagaaatt taatttaaag gttgttcaaa atcatcaaaa aggtagtttt
    11941 tctaagtttt taacatcaaa aaataatcat aagaaaataa agagagtgtt tattccaggt
    12001 tgtagcttaa gtggatataa taatgaaatt atttttaaaa taaaggacta cttaatagaa
    12061 aatttaggag aagtagagat acttctaaaa tgttgtggaa aaccttcaaa ggatattgga
    12121 aacttagaat tatttgaaag caatttaaat gatatagaga ataatctaaa aatcaaggaa
    12181 gttgatgaaa taatagtagc ctgtgaaaat tgttataaga cttttggatc agcttttaaa
    12241 gatattaaag taataacact ttgggaagtt ataaatgaat ttggagtacc aaaggaacta
    12301 aaaaacaaat attcagattt aaaagaagaa tttgccttgc atgatccatg ccctataaaa
    12361 aaagaagaac aaattcacga agctgttaga agcatactta aagatttagg aattaaaata
    12421 gtagaatttg agaagaatag aagtaaaact gaatgctgtg gttgtggagg aatggtagga
    12481 gttacaaata aggaattatg gctaagtcaa aaaaataaaa gagctaattc tacagaatgt
    12541 gaaaatatag tttcttattg tgaatcctgc gtaaatacct ttaaatcttc agataaaaat
    12601 gcactacaca tattagactt tatttttaat gaggatgtta ttaatggcaa agctaaaagt
    12661 caaaagaata taagcacaat taatcaatgg ataaatagaa ttaaattagc aaacaaatta
    12721 ggaaaagaga gttaatttaa aattggggta gagactacca taaaagcttg ggaaaccttg
    12781 tacttttaga tatatttatt aaggagctaa catttatata agtggagagt agttcgcata
    12841 cttatctaat aaaaataaaa gcaaatgaaa gtttaaaata gagggggaga aaaaaataat
    12901 gttagatact tatggaagaa agtatgtaaa tccaataata gatttaggtg ctaatacatt
    12961 attaaagtta agattaacac caaatggagt tagtataata gctctaatta taggagtttt
    13021 aagttctgta tttctttatt tcgataaatt gattttatca gtagtttttc tttggatatc
    13081 aggatattta gattctgttg atggtgctat ggcaagaaaa aaggatttaa ctagcccttt
    13141 tggaacactt ttagatataa cttttgatag aattgttgaa cttagcataa tatttgtctt
    13201 agctttaaaa tttcaaaata gtagattata tcttttaata ttaaccatga tgattttaat
    13261 atctatgacc atatttttaa cagtaggagc cttagctcaa aataatggta tgaagtcttt
    13321 tagataccaa gcaggacttg cagaaaggtc agaaggattt atttgttttt catttatgat
    13381 tttactatca tctataaaat taaatttagt tacaaatata ttttccttaa taatatttat
    13441 aactataata caaagagctt tagaggctaa aaagctttta aaataatata tatttatagt
    13501 atttatgcct ttggcaagaa cacccgtgat ttcaatcatg ggtgttcttg ccgcgacata
    13561 tgctaaaagt ataaaccgtc taacacttgc aaataagtta tattttagtt ttaaatattt
    13621 tacatatgtt aaattaaata ataatataat taaagaggtg gtaataatgg ctcaagtcaa
    13681 gcaaggcaga ggttatgtgt attgtattca atatcatata gtatggtgtg t
//
LOCUS       DAEVGI010000060        13180 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEVGI010000060 DAEVGI010000000
VERSION     DAEVGI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.060"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            72..1088
                     /gene="asrA"
                     /locus_tag="K8M77_001447"
     CDS             72..1088
                     /gene="asrA"
                     /locus_tag="K8M77_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit A"
                     /protein_id="HBI7085946.1"
                     /translation="MGLRLTKDNFDKIIDCLKKEYKIYAPKVMEGKGRFSDTDMTRYG
                     EIDSINDIEFSKKSDFSYKEVLLPITQTLFFFTEDKFSEASVEEKNILIFLRSCDMHS
                     LRRIDDIYLRNGFEDPYYKKLREKAKFIVMGCENSFDNCFCVSMGTNKTDEYSAYIGL
                     DNDCVLLDIKDEELSKIFEAVESNKEEVSPKFVEENETKVEVPEGIDLNIIKSTVWNE
                     YSERCIACGRCNFVCPTCTCFTMQDVFYKDNENAGERRRVWASCQVDGYTDMAGGHSF
                     RKDKGQRMRFKVMHKVYDYKKRWGYHMCVGCGRCDDICPEYISFSECVNKLKDAVEEV
                     KDNA"
     gene            1081..1872
                     /gene="asrB"
                     /locus_tag="K8M77_001448"
     CDS             1081..1872
                     /gene="asrB"
                     /locus_tag="K8M77_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit B"
                     /protein_id="HBI7085947.1"
                     /translation="MHNEYTPFISKILNVKKHTDIEYTFRMEFKGDVKPGQFFEVSLP
                     KFGEAPISVSGIGEDYVELTIRRVGVVTNEIFEKYEGDKLFLRGPYGNGFDVNNYKGK
                     EVIVVAGGTGLSPVKGIVDYFSQNPKDAESFTLISGFKGPKDILFKDDMKEWEKNMNM
                     IITVDSAEEGYEGNTGLVTKYIPELEIKDMDNVQVIVVGPPMMMKFTVLEFLKRGIKE
                     ENIWISQERKMCCGLGKCGHCKIDDTYICLDGPVFNYTKGKLLID"
     gene            1886..2866
                     /gene="asrC"
                     /locus_tag="K8M77_001449"
     CDS             1886..2866
                     /gene="asrC"
                     /locus_tag="K8M77_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBI7085948.1"
                     /translation="MSLDINTKKLKKNAFRVTKRRGYTASRIRVPGGHLDAKLLLNIQ
                     EIADTYGDGTVHITTRQGFEIPGIDMKDIPEVNKKLQPIIEALEINQENQGDGYNAAG
                     TRNVSACIGNNVCPFANYNTTNFAKKIEKAIFPNDLHFKIALTGCPNDCIKARMHDFG
                     IIGMTEPQYERNRCVSCGACVRACKKKATGALSFENFKVVRDGSKCIGCGECVMNCPT
                     NAWTRSKEKYYRLAIMGRTGKKNPRLAEDFILWVDEDSIIKIILNTYKYVTEYIDKNA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVTLSEKALMKNNVYWSGIHYTNGF"
     gene            3048..4250
                     /locus_tag="K8M77_001450"
     CDS             3048..4250
                     /locus_tag="K8M77_001450"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBI7085949.1"
                     /translation="MIGVIGVKRNVDIAIREKLALYPKKHKKYVGELLNSFKEVVILN
                     TCNRTEIYFNCTEEISEDEIFDKIFNVFNWNDDLKKYMFLSKEKRAVTHLMEVICGFH
                     SRILGEDQILGQIKDAYKTAISDNSISSELQKMFEIAIACGKKFKTKCKMFEVPVSSV
                     SISINSALLKGCRKFMVLGYGEIGKLAIKHLLSHKVECIYLIVRDKSKASDLEGEIVE
                     ILDFNEKNHVINEVDCIVSCTAAPHTVVRNEDIKTEGDIIHIYDLAVPRDVDKELSEK
                     ERVILKDIDEISKIDDKNKKIRKERMEEYKHIVEESIEEFLNWLKIREVSSKIRNIKI
                     RENEICSERIKTFSNKGNGENAKLAERMIKSTADAYVNRAIELLKSEALKGSDSSCAE
                     IIEKIFLT"
     gene            4214..4876
                     /locus_tag="K8M77_001451"
     CDS             4214..4876
                     /locus_tag="K8M77_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI7085950.1"
                     /translation="MCRNNREDIFNLVPINIISSKFHVKVIGGGKAATIKVKGLLEKG
                     CYVHILSKEFSKEILDIENKNLKLEKGTYYKEFIKDAHLIIIAVDESSLVDKISKDCE
                     KEYKLYLNASNYKEGMVAIPYSRFYENLGFSISSKLGSPRITRSIGEEVSNIVKENDI
                     YSIKVAKIREKAKKEELKDNIIEIISSKEFKKALLEKREYEYLRNLLGEELANSLTLG
                     LK"
     gene            5021..5896
                     /gene="hemC"
                     /locus_tag="K8M77_001452"
     CDS             5021..5896
                     /gene="hemC"
                     /locus_tag="K8M77_001452"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBI7085951.1"
                     /translation="MELIIATRKSKLAQVQTEKVMELLKKKENVDSKKLLVMTEGDRR
                     LDVSLNKIGGKGLFVKEIELALLNKEAHGAVHSMKDVPFELPSEFELVAMPEREDIRD
                     AFVSLNGSTLSNLRKGARIGTSSIRRAEQLKLFRDDLEIVPIRGNVQTRIKKITEENL
                     DGIILAAAGLKRLGMEDVISDYFDPKVFLPAIGQGALGIECLKDGEFNDYFKALDSKE
                     VRTTVEAERSFMKILNGGCHSLIGAYSEVKDNDLYMIGTFTVNNRIVKKDILGNKEDN
                     ILLGKKLAEKILGEV"
     gene            5905..7383
                     /gene="cobA"
                     /locus_tag="K8M77_001453"
     CDS             5905..7383
                     /gene="cobA"
                     /locus_tag="K8M77_001453"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI7085952.1"
                     /translation="MSKKAYIIGAGPGDEELLTLKAINALQKCTAVLYDRLVGNNILN
                     YLNDDCEIYYCGKEPGCHYKTQEEINESIVELAKKGHIVGRVKGGDPYVFGRGGEEVL
                     ALVEENIPFEVIPGVTSPISVLNYAGIPITHRGLAQSFHIVTGMSARTLNVNWKALSK
                     ENGTLVFMMGLSNLETIVEKLLENGKDIETPCGVVMRGTTSKQRKVIGTLENICKKVR
                     EAKLESPCIIVVGDVVSLNEKLSWYEKLPLFGANICLTRSKEQSKEIKWKLKELGAEV
                     TEINSIKIKETSENLDEYINTLEKYDHIVFTSVNAVNVFFDYLVKKRVDIRKIKADFA
                     VLGKATKKALIARGIVPSIMAHSFTAEGLFEVLKDNIKEGEEVLIPCSSLSREYLFDN
                     LASLGAKCHRVNIYDTVCGDVKNPRAFKEVDMVLYTSPSTVKNMIDMIGLESLKEKVS
                     IAIGPITLKALNESGIEGKMCKTHCGDGFLSEIEGIWQEVKK"
     gene            7440..8405
                     /gene="hemB"
                     /locus_tag="K8M77_001454"
     CDS             7440..8405
                     /gene="hemB"
                     /locus_tag="K8M77_001454"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBI7085953.1"
                     /translation="MIKRGRRLRINKEMRDLVRENILTANDFIFPIFVAEGNNIKKEI
                     SSLPGNYHVSLDRLNEIVDEVVALNIKGVIIFGLPEHKDACGSEAYSDNGIVQKAIRK
                     LREDYENLLIITDVCMCEYTSHGHCGIIEGKDVDNDKTLSFLDKIAVSHAKAGAHMVA
                     PSDMMDGRILSMRNALDEAGFVNVGIMSYSAKYCSAFYGPFREAANSAPQFGDRKTYQ
                     MDPANSREAIKEVEQDIEEGADIVMVKPALSYLDIVKEVRNKVDVPVCVYNVSGEFAM
                     VKAAAKLGLINEKQVALEMLLSMKRAGADMIITYYAIEAAKWLQE"
     gene            8464..9747
                     /gene="hemL"
                     /locus_tag="K8M77_001455"
     CDS             8464..9747
                     /gene="hemL"
                     /locus_tag="K8M77_001455"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBI7085954.1"
                     /translation="MNMDRNKEIFEESKKYMPGGVNSPVRSFGSVGINPPVIKSGKGA
                     MIKDENGNEYIDFVLAWGPMILGHCDEDVVEAIKKTSEESIAFGASTKLELDLAKILC
                     ETLDNVDMIRMVNSGTEATMSAVKLARGYTKKDKIIKFAGCYHGHFDGFLIEAGSGVL
                     TEGIPGCLGVPEESIKNTLIGIYNDEKQVEELFEKYGNDIAGIIIEPVAGNMGVVKCD
                     PKFMRKLRELCDKYEALLIFDEVMCGFRVAYKGAQTLFDVKPDLVTYAKIMGGGLPCG
                     AYGGRREIMENLSPLGGVYQAGTMSGNPIVMSAGLATVKKLYENPSYYDHIEKIGSKL
                     EKGIIEIAKKKGLGLVVNRQGGMMTLFFTDLKEVKCYDDVKTCDGERFKRYFLHMLNK
                     GFNIPPSQFEAMFLSVKHTEEHIDKFLEAFESFEG"
     gene            complement(10052..11482)
                     /gene="cls"
                     /locus_tag="K8M77_001456"
     CDS             complement(10052..11482)
                     /gene="cls"
                     /locus_tag="K8M77_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI7085955.1"
                     /translation="MHLLINIIFLINIIFIISIIFIERRNPQTTWAWILILTFLPILG
                     FIIYILFGQNITREKNFKRKILDDKTKQKYLNSFKSHYKLDNISLKYKDLIMMNFNND
                     NSTYTQRNDIDLYFDANSLFQEMIYEINKAEKFIHMEFYIFKSDEIGKKILQALTKKA
                     KEGVEVKLLVDSIGNSIHKKDIDKLKAAGGDFKIFFPGFCKYINLRINYRNHRKILII
                     DSKVAFLGGFNIGDEYLGKDKNIGNWRDTHTKIKGLAINDLEARFLLDWSYANESDLD
                     IDLKKYFINPHSTNLPNNIIGAQIVSSGPDHTEQQIKNGYFKIINSAKKNLFIQTPYF
                     VPDEPMLEALRLAALSGVDVKIMLPGNPDHKFMEWIANSYFESLLNAGVKIYLYEKGF
                     LHAKTIVADSSICSVGTANMDIRSFSLNFESNIFIYNEAISKSMEEQFFKDLKVCTKV
                     TLESFEKRSIISRIGESIIRLVSPLM"
     gene            complement(11561..13102)
                     /locus_tag="K8M77_001457"
     CDS             complement(11561..13102)
                     /locus_tag="K8M77_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relA/spoT family protein"
                     /protein_id="HBI7085956.1"
                     /translation="MELKVFQFTKGTLELLNEMKKDLAYSANLLDDFFYSLLENSCEG
                     FFNISTRVKSASSLKEKIIRQNYYIKYNSPTDLFRNLSDLIGVRIECRFIEDEEHIFK
                     FIKTIFNCTNKDGFSYSSQNPNIFLDLREDQPLKQKNGFELYRIDGFILNEEEKFNFE
                     LQIKSMVNNFWGEIEHKIIYKNYNMILGDKFYKNILNSIKNNLCLIDNQLLTIFNHVN
                     SHMNTCTGIGLKKEGIEILLSKMIYDIYSSKVKHDLGISVDFRNACDIIIDYIFTKNN
                     CNTSQEYYNTFVNTSVRLNEVFKDSISFKNKLSIGAEPLCFDSEFSNSIGNKLAHAMN
                     YDFHWNLFFKILFQIEPGNNREDFYSFIRYLETIFSNRDSYLNLYLTFSAEEVSIIKE
                     DILSSLNKAFLEIDSIKFIYRDKLSEIFNHIDDYVKFLCEGIDSYENYVSHKHLYTKY
                     LYLTILSSFNMDLDKSSLLEFASELKNSKCKLRISYKGIKLLTSTPENCKVNTIELLE
                     QIYIR"
BASE COUNT         5182 a         1358 c         2374 g         4266 t
ORIGIN      
        1 aaagaaaaac actttcatta ttataattta tttaatttca aaataaagtt aaatttaaga
       61 ggtgttttat tatgggttta aggcttacaa aagacaattt tgataagata atagactgtt
      121 taaaaaaaga atataagata tacgctccta aagttatgga aggtaaagga agattttcag
      181 atactgacat gacaagatat ggagaaatag attctataaa tgatattgag ttttcaaaga
      241 agtcagattt ctcatataaa gaagttttac ttccaataac tcaaacttta ttcttcttta
      301 ctgaagataa atttagtgaa gcaagtgtgg aggaaaagaa tatattaatt ttcttaagaa
      361 gctgtgatat gcattcatta agaagaattg atgatatata cttaagaaat ggttttgaag
      421 atccttatta caaaaaacta agagaaaaag ctaagtttat agtaatggga tgtgaaaata
      481 gctttgataa ttgtttctgt gtaagcatgg gaactaataa aacagatgaa tattcagcat
      541 atataggatt agataatgat tgtgttttat tagatataaa agatgaagag ttatcaaaaa
      601 tctttgaagc agtagaaagt aataaagaag aagtttcacc aaaatttgta gaagaaaatg
      661 aaactaaggt tgaagttcca gagggaattg atttaaatat aatcaaatca actgtatgga
      721 atgaatactc agaaagatgt atagcttgtg gaagatgtaa ctttgtatgt ccaacatgta
      781 cttgtttcac aatgcaagat gtattctaca aagataatga aaatgcaggt gaaagaagaa
      841 gagtttgggc ttcatgtcaa gtagatggat atacagatat ggctggtgga catagcttta
      901 gaaaagataa aggtcaaaga atgagattta aagttatgca caaagtatat gactataaaa
      961 agagatgggg ataccatatg tgtgttggat gtggtagatg tgatgatatc tgtccagaat
     1021 acatatcttt ttcagaatgt gtaaataaac ttaaagatgc tgtagaagag gtgaaggata
     1081 atgcataatg aatatactcc ttttatatca aaaatattaa acgtaaaaaa acatactgac
     1141 atagaatata cttttagaat ggaatttaaa ggcgatgtta agccaggaca attctttgaa
     1201 gtttctcttc caaagtttgg agaggctcca atatcagtaa gtggtatagg agaagactat
     1261 gtggaactta caataagaag agttggagta gtaacaaatg aaatatttga aaaatacgaa
     1321 ggagataaac ttttcttaag aggaccttat ggtaatggat ttgatgttaa taactataaa
     1381 ggaaaagaag ttatagttgt tgctggagga acaggacttt caccagttaa gggaatagta
     1441 gattatttct cacaaaatcc aaaagatgca gaaagcttta ctcttataag tggatttaaa
     1501 ggaccaaagg atattctttt caaagatgac atgaaagaat gggaaaagaa tatgaatatg
     1561 atcataactg ttgactctgc tgaagaaggt tatgaaggaa atacaggact tgtaactaag
     1621 tatattcctg aattagaaat aaaagatatg gataatgttc aagttatagt tgttggtcct
     1681 ccaatgatga tgaagtttac agttttagaa ttcttaaaaa gaggaattaa agaagaaaat
     1741 atttggatat ctcaagaaag aaaaatgtgt tgtggattag gtaaatgcgg acactgtaag
     1801 atagatgaca catacatatg cttagatgga ccagtattca actacactaa gggtaaactt
     1861 ttaatagatt aggaggaaaa ggactatgtc tttagatata aacactaaaa aacttaagaa
     1921 gaacgctttt agggtaacta aaagaagagg atatacagca tcaagaataa gagttccggg
     1981 tggacactta gatgctaagt tattacttaa cattcaagaa atagctgata cttatggaga
     2041 tggtacagtt catatcacta ctagacaagg ttttgaaata ccaggaatag atatgaaaga
     2101 tataccagaa gtaaataaaa aacttcaacc tattatagaa gctttagaaa taaatcaaga
     2161 aaatcaagga gatggataca atgcagcagg aacaagaaac gtttcagcat gtataggaaa
     2221 taatgtttgt ccatttgcta attacaatac aacaaatttt gctaagaaaa tagaaaaagc
     2281 aatattccca aatgatttac acttcaaaat agctttaact ggatgtccaa atgattgtat
     2341 taaggctaga atgcatgact ttggtattat aggtatgaca gagccacaat atgaaagaaa
     2401 tagatgtgta tcttgtggag catgtgttag agcttgtaaa aagaaagcta caggagcttt
     2461 aagttttgaa aacttcaaag tagttagaga tggaagcaaa tgtattggtt gtggagaatg
     2521 tgttatgaat tgcccaacaa atgcgtggac aagaagtaaa gaaaaatact acagattagc
     2581 tataatgggt agaacaggaa agaaaaatcc aagattagca gaagatttta tcctttgggt
     2641 agatgaggat agtatcataa aaataatctt aaatacttat aaatatgtta cagaatatat
     2701 tgataaaaat gctccaggtg gaaaagagca cattggatac ataatagaca gaactggatt
     2761 tatggaattc aagaaatggg ctttagaagg agtaacttta tctgaaaaag cattaatgaa
     2821 aaacaacgtt tattggagcg gaatacatta tacaaacgga ttttaataaa aaagcactct
     2881 atttataggg tgcttttatt tatataagtt attaattata ataaatatgt atttttaata
     2941 taactaatac aattatttca ttgggcatat ataattttta tgatattata aaatagagtg
     3001 ttttaataaa attttaacat atgatataaa aacttaggag gcagttcatg ataggagtta
     3061 taggggttaa aaggaatgta gatatagcaa taagagaaaa attagcctta tatcctaaaa
     3121 aacataagaa atacgtagga gaattattaa attcatttaa agaagttgta atattaaata
     3181 cttgtaatag aacagagatt tattttaatt gcactgaaga gatttcagag gatgaaattt
     3241 ttgataaaat ttttaatgtg ttcaattgga atgatgattt gaaaaaatat atgtttttat
     3301 caaaggaaaa gagagcagta acccatctta tggaggttat ttgtggtttt cattcaagaa
     3361 ttttaggtga agatcaaatc ctaggacaaa ttaaagatgc ttataaaaca gctatttcag
     3421 ataatagtat ttcatcagaa ttacaaaaga tgtttgaaat agctattgct tgtggtaaaa
     3481 aatttaaaac aaagtgtaaa atgtttgaag ttccagtttc atcagtatca atatcaataa
     3541 atagtgcctt attaaaagga tgtagaaaat ttatggtatt aggatatgga gaaattggga
     3601 agttggcaat taagcacctt ttaagccata aagtagagtg tatttattta atagtaaggg
     3661 ataagagtaa ggcaagtgat ttagaaggtg aaattgtaga aattttagat tttaatgaga
     3721 aaaatcatgt tataaatgag gttgattgta tagtttcttg tacagcagca cctcatacag
     3781 tagttagaaa tgaagatata aaaactgaag gtgacataat acatatatat gatttagctg
     3841 ttccaagaga tgtagataag gaattatcag aaaaagagag agtaattctt aaagatatag
     3901 atgaaataag taagattgat gataagaata aaaaaataag aaaagaaaga atggaagagt
     3961 ataagcatat agttgaagag agcatagagg aatttttaaa ctggcttaaa ataagagaag
     4021 tttcaagtaa aataagaaac ataaaaataa gagaaaatga aatttgtagt gaaagaataa
     4081 agactttttc taataaggga aatggagaaa atgctaaatt agcagaaaga atgataaaaa
     4141 gtacagctga tgcttatgtt aatcgggcaa tagaactttt aaaaagtgaa gccttaaagg
     4201 gaagtgatag ttcttgtgca gaaataatag agaagatatt tttaacttag taccaataaa
     4261 cattataagt agtaaatttc atgttaaggt cataggtgga ggaaaagcag ccaccataaa
     4321 ggttaaagga ttattagaaa aaggttgtta cgtacatatt ttatctaagg aattttcaaa
     4381 ggaaatctta gatattgaaa ataaaaattt aaaacttgaa aagggaacct actataaaga
     4441 gtttataaaa gatgctcatc ttataataat agctgtggat gaaagttctc tggtagataa
     4501 gataagcaag gattgtgaga aagaatataa actatattta aatgcatcaa attataaaga
     4561 gggtatggta gctattcctt atagtagatt ttatgaaaat ctaggattta gtataagcag
     4621 taaattagga tcacctagaa taactaggtc tataggagaa gaagtatcaa atattgttaa
     4681 ggaaaatgac atatactcaa ttaaggtagc taagattaga gaaaaagcta aaaaagaaga
     4741 gttaaaggat aatataatag agattatttc aagcaaagag tttaaaaagg cccttttaga
     4801 aaaaagagaa tatgaatact taagaaatct tttaggagaa gaattagcta attccttaac
     4861 tttaggatta aagtaaatat aagaggatac tgtatgaaag ttttatcaaa attatttcga
     4921 tacaccactt taactttatt aaggtaagaa tttgttttaa gtgctttaaa aaataagcta
     4981 aatttattat aaaaataatc aaaatgtgag gtttggatta atggaattaa taatagcgac
     5041 tagaaaaagt aagttagcac aagtgcaaac tgaaaaagtt atggaacttt taaagaaaaa
     5101 agaaaatgta gatagtaaaa agcttttagt tatgacagaa ggtgatagaa gactagatgt
     5161 ttcattaaat aaaataggtg gaaagggtct atttgtaaag gaaatagaac tagctctttt
     5221 aaataaagaa gctcatggag cagttcatag tatgaaagat gttccttttg aacttcctag
     5281 tgaatttgag cttgttgcaa tgccagagag agaagatata agagatgctt ttgtatcttt
     5341 aaatggatca actttatcta atttaagaaa aggagctaga ataggaacta gcagtataag
     5401 acgtgcagag cagttaaagt tatttagaga tgatttagag attgttccaa ttagagggaa
     5461 tgttcaaact agaatcaaaa aaataactga agaaaattta gatggaataa ttttagcggc
     5521 agcaggatta aaaagacttg gtatggaaga tgtaataagt gattattttg atccaaaggt
     5581 atttttacct gctattggtc aaggagcttt aggaatagag tgcttaaaag atggagaatt
     5641 taatgactat tttaaagctt tagatagcaa agaagttaga actactgttg aggctgaaag
     5701 aagctttatg aagatattaa acggaggatg tcatagtctt ataggtgctt attcagaagt
     5761 taaagataat gacttatata tgataggtac atttactgta aataatagaa tagttaaaaa
     5821 agatatatta ggaaataaag aagataatat attattagga aaaaaacttg cagaaaaaat
     5881 attaggagag gtctaggagg aactatgagt aaaaaagcat atataatagg ggcaggtcct
     5941 ggagatgaag agttattaac acttaaagca ataaatgcat tacaaaaatg cacagctgtc
     6001 ttatatgata gattagttgg aaacaatata cttaattact taaatgatga ctgtgagatt
     6061 tactattgtg gaaaagagcc aggatgtcat tataaaacac aggaagagat aaatgaaagt
     6121 atagttgaac ttgctaaaaa aggtcatatt gttggtagag taaaaggtgg agatccctat
     6181 gtttttggaa gaggtggaga agaagtatta gccttagtag aagaaaacat accttttgaa
     6241 gttattccag gggttacttc acctatttct gttttaaatt atgcagggat accaataaca
     6301 catagaggat tagcacagag ttttcatatt gtaacaggaa tgtcagcaag aactttaaat
     6361 gtaaattgga aagctttatc aaaagaaaat ggaaccttag tatttatgat ggggctttca
     6421 aatttagaga caattgttga gaaactttta gaaaatggta aagatataga aactccttgt
     6481 ggagtagtaa tgagaggaac aacttcaaag caaagaaagg ttattggaac attagaaaat
     6541 atatgtaaaa aggttagaga agctaagtta gagtcacctt gtataatagt tgttggtgat
     6601 gttgtttcat taaatgaaaa actttcatgg tatgaaaaat tacctctatt tggagctaat
     6661 atttgtttaa caagatctaa ggaacaatct aaagagatta agtggaaatt aaaagagtta
     6721 ggtgcagaag taacagaaat aaactctata aaaataaaag aaacttcaga aaatttagat
     6781 gaatatatta atactttaga aaaatatgat catatagtat ttacttcagt aaatgctgtt
     6841 aatgtattct ttgattattt agtgaaaaaa agagttgata taagaaaaat aaaagcagat
     6901 tttgctgtac taggaaaagc aactaaaaaa gctttaatag ctagaggaat tgtaccaagc
     6961 ataatggctc attcatttac agcggaaggt ttatttgaag ttttaaaaga taatattaaa
     7021 gaaggagaag aagtcttaat tccatgctct tctttaagca gagaatattt gtttgataat
     7081 ttagcttctt taggagcaaa atgtcataga gttaatattt atgatactgt atgtggagat
     7141 gttaaaaatc caagagcttt caaggaagtt gatatggtat tatatacaag tccttcaacg
     7201 gttaaaaaca tgattgatat gattggactt gaatctctta aagagaaagt aagtatagct
     7261 ataggaccta taaccttaaa agctttaaat gaaagtggaa ttgaagggaa aatgtgcaaa
     7321 acacattgtg gggatggatt tttaagtgaa attgaaggta tatggcaaga ggttaaaaaa
     7381 taaatttgtg gtaaaattaa ttttataggt aaattacaaa taaattagga ggaaagatta
     7441 tgataaagag aggaagaaga cttagaataa ataaggaaat gagagatctt gttagagaaa
     7501 atattttaac agcaaatgat tttatatttc ctatatttgt agctgaagga aataacataa
     7561 aaaaggagat aagttctcta ccaggaaact atcatgtatc tttagataga ttaaatgaaa
     7621 tagttgatga agtggtagct ttaaatataa aaggtgtaat tatatttggt cttccagaac
     7681 ataaggatgc ttgtggatca gaggcttatt ctgataatgg aatagttcaa aaggctatta
     7741 gaaaattaag agaagattat gagaacttat taataataac tgatgtttgt atgtgtgaat
     7801 atactagtca tggtcattgt ggaataatag aaggaaaaga tgtagataat gacaagactt
     7861 taagtttctt agataaaata gcagtttcac atgctaaggc tggtgctcat atggtagctc
     7921 cttcagatat gatggatgga agaatattat ctatgagaaa tgctttagat gaagctggat
     7981 ttgttaatgt tggaataatg agttattctg caaaatattg ctcagctttc tatggacctt
     8041 tcagagaagc tgctaattca gcaccacaat ttggagatag aaaaacttat caaatggatc
     8101 ctgcaaactc aagagaagcc ataaaagaag tagagcaaga catagaagag ggtgcagata
     8161 tagttatggt taaacctgcc ttatcatatt tagatattgt taaggaagta agaaataaag
     8221 tagatgtacc agtttgtgtt tataatgtaa gtggtgaatt tgctatggtt aaggcagcag
     8281 ctaaacttgg acttataaat gaaaaacaag tagctttaga aatgctttta tcaatgaaaa
     8341 gagcaggggc agatatgata ataacttatt atgctataga agctgctaaa tggttacaag
     8401 aatagtttaa gactataata taatcaaaaa atagatttga ttaacaatct tatatttagg
     8461 agaatgaata tggacagaaa taaagaaata tttgaagaat caaaaaaata tatgccaggt
     8521 ggagttaata gtcctgtaag atcttttgga agtgttggaa taaatcctcc agtaataaaa
     8581 agtggtaaag gagctatgat aaaggacgag aatggaaatg aatatattga ctttgtttta
     8641 gcttggggac caatgatact aggtcattgt gatgaagatg tagtagaagc aataaagaaa
     8701 acttcagagg aaagtatagc ctttggtgct tcaacaaaat tagaattaga cttagctaag
     8761 attttatgtg aaacattaga caatgttgac atgataagaa tggtaaactc aggtacagag
     8821 gctactatga gtgctgtaaa gttagcaaga ggatacacaa agaaggataa aataattaag
     8881 tttgcaggat gttatcatgg acactttgat ggattcttaa tagaagctgg atcaggagta
     8941 ttaacagaag gaattccagg atgcttaggt gttccagagg aaagtataaa gaatacttta
     9001 ataggaatat acaatgatga aaagcaagtt gaagaattat ttgaaaaata cggaaatgat
     9061 attgctggaa taataataga gccagttgct ggaaatatgg gtgttgttaa atgtgatcct
     9121 aagtttatga gaaaacttag agaattatgt gataaatatg aagcacttct tatatttgat
     9181 gaagttatgt gtggatttag agtagcatat aaaggagcac aaactttatt tgatgttaag
     9241 ccagatttag ttacttatgc taaaataatg ggtggtggac taccttgtgg agcatatggt
     9301 ggaagaagag agataatgga aaacttatca ccactaggtg gagtttatca agctggaaca
     9361 atgtcaggaa atccaatagt aatgagtgca ggacttgcaa cagttaaaaa actttatgaa
     9421 aatccatcat actatgatca tattgaaaaa ataggatcaa aacttgaaaa ggggattatt
     9481 gaaatagcaa agaaaaaagg tttaggcctt gttgttaata gacaaggtgg catgatgact
     9541 ttattcttta ctgatttaaa agaagttaag tgttatgatg atgtaaaaac ttgtgatgga
     9601 gaaagattta agagatactt cttacatatg ttaaacaaag gatttaacat accaccatca
     9661 caatttgagg caatgttctt aagtgtaaaa catacagaag aacacataga taaattctta
     9721 gaagcttttg aaagctttga aggataataa aatttaaaaa gttttaaatc aatgctgatt
     9781 tttaggtata tatttaaaaa aagacttaat ttaataggaa gctttattac aaatttaata
     9841 tacttatatg tttttgtatt agaaattttc ttcttaatta agatatttat atacctataa
     9901 atcagcttta attttataat tggaaatttt aattaggtgg ggtatttatg agagaaagag
     9961 tgaaattaaa aaaagacaaa gaatgtgatg tttttgtaat gggagctgga attaataaaa
    10021 aatacagtgt atgatcatac actgtatttt tctacattaa aggagatact aatcttatta
    10081 tggattctcc aattcgtgat ataatagatc gtttttcaaa actttcaaga gttactttag
    10141 tacacacctt taggtcttta aagaattgct cctccataga ttttgaaata gcttcattat
    10201 atataaatat attgctttca aagtttaaag aaaagcttct tatatccatg ttagctgttc
    10261 ctacactaca tatagaagag tctgccacta tggttttagc gtgaagaaaa cccttttcat
    10321 ataagtagat ctttacacca gcatttaata aggattcaaa ataagagttt gctatccatt
    10381 ccataaattt atgatcaggg ttgccaggta gcattatttt tacatcaact ccagataagg
    10441 cagcaagtct taaagcttca agcataggtt catcagggac aaaataagga gtttgtatga
    10501 aaagattttt cttagctgaa tttataattt taaaatatcc attttttatt tgttgttcgg
    10561 tatggtcagg accagaggag actatttgag ctccaataat attatttggc aaattagttg
    10621 aatgaggatt tataaaatat ttttttaaat ctatgtccaa atctgattca ttagcataac
    10681 tccaatctaa aaggaatctt gcttctagat cattaatagc aaggcccttt attttagtat
    10741 gagtatcacg ccaattacca atatttttgt cttttccaag atattcatca cctatattaa
    10801 atccacctaa aaaagcaact ttgctatcta taataagtat ctttctatga tttctatagt
    10861 taattcttaa gtttatatat ttgcaaaagc caggaaaaaa tattttaaaa tctccaccag
    10921 ctgcctttaa tttatctata tcttttttgt gtatagagtt accaatagaa tctactagta
    10981 attttacttc aacaccttct ttggcttttt tagttagggc ttgtaagatt tttttaccta
    11041 tttcgtcgct tttaaaaata taaaattcca tatgaataaa tttttctgct ttatttattt
    11101 cataaatcat ttcttgaaaa agactattag catcaaaata taaatctata tcattacgtt
    11161 gagtgtaggt tgagttgtca ttattaaaat tcatcattat taaatctttg tattttaaag
    11221 aaatattatc taatttgtaa tgagacttaa atgaatttaa atatttttgt tttgtcttgt
    11281 catctagaat ttttctctta aagttttttt cgcgggtaat attttgacca aataaaatgt
    11341 agattataaa tcctaatatg ggtagaaagg ttaatattaa aatccaagcc caagtagttt
    11401 gggggtttct tctttcaata aatattattg aaataataaa tataatattt attaaaaaga
    11461 ttatatttat aagtaaatgc ataataattc ctcctttgta tttagaaagg tttatcagtc
    11521 taaatttttt aatatatatt agattgataa actaaaaatg ttaccttata tatatttgtt
    11581 ctaaaagttc aattgtgttg actttacaat tttcaggggt agaggttaat agctttattc
    11641 ccttatagct tattctaagt ttacatttgc tatttttaag ctcactagca aactctagta
    11701 gggaactctt atctaaatcc atattaaagg aacttaatat agtaagataa agatactttg
    11761 tgtaaagatg tttatgactt acataatttt cataagaatc tatgccctca cataggaatt
    11821 taacataatc gtctatatga ttaaatattt cagaaagttt atccctataa ataaatttta
    11881 tagaatcaat ttccaagaat gccttattta aagaagataa aatatcttct tttattattg
    11941 atacctcttc agctgaaaaa gttaaataaa gatttaaata agaatctcta ttagagaaaa
    12001 tagtctctaa gtatcttata aatgaataaa aatcttctct attgttacca ggctctattt
    12061 gaaataatat tttaaagaat aagttccaat ggaaatcata attcatagca tgggctagtt
    12121 tatttccaat tgaattagaa aattctgagt caaaacataa gggctcagca cctatagata
    12181 atttgttttt aaaagatatg gaatccttaa aaacttcgtt taatcttact gaggtattaa
    12241 caaaagtatt ataatattct tgagaagtat tacaattatt tttagtaaat atgtaatcta
    12301 ttattatgtc acaggcattt ctaaaatcaa cagatatacc taaatcatgt tttactttag
    12361 aagagtatat atcatatatc attttagaaa gtaaaatttc aatgccttcc ttctttaatc
    12421 caattccagt acaagtattc atatgagaat taacgtggtt aaatatggtt aaaagttgat
    12481 tatctattag acataaatta tttttaatag aattaagtat atttttataa aatttatctc
    12541 ctaaaatcat attatagttt ttatatatga ttttatgttc aatttcaccc caaaaattat
    12601 ttaccataga ttttatttga agttcaaaat taaacttttc ttcttcattt agaataaaac
    12661 catctattct atataattca aaaccatttt tttgttttaa tggttggtct tctcgaaggt
    12721 ctaaaaatat atttggattt tgtgatgagt aagaaaatcc atctttatta gtgcaattaa
    12781 atattgtttt tataaattta aatatatgtt cctcatcttc tataaatcta cattctattc
    12841 tgacccctat taaatcagat agatttctaa ataaatcagt aggggaatta tattttatgt
    12901 aataattttg tcttattatt ttctccttta aactagaagc tgattttact ctagttgata
    12961 tattaaaaaa tccttcacaa gagttctcta gcagggagta gaaaaaatca tcaagtaaat
    13021 ttgcactata ggctaaatct tttttcattt cattaagtaa ttctaaagta ccttttgtaa
    13081 attgaaaaac ttttaattcc aaatttaaaa caccgtcctt gttagtttat aaataaagtc
    13141 agtatatttt aataatacca acatatagta agtataacaa
//
LOCUS       DAEVGI010000061        13059 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEVGI010000061 DAEVGI010000000
VERSION     DAEVGI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 13059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13059
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.061"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..218
                     /locus_tag="K8M77_001458"
     CDS             <1..218
                     /locus_tag="K8M77_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085957.1"
                     /translation="SFGENEFPLKYLSEEELEELGFKKSCCGSRGGCPKKANGTCGGC
                     SSKGGCQGGCSKNGCGCSNGSGCCKNK"
     gene            complement(257..1843)
                     /locus_tag="K8M77_001459"
     CDS             complement(257..1843)
                     /locus_tag="K8M77_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7085958.1"
                     /translation="MNNIKFDDLGLKESLLKAIKDMGFEEPSQIQAESIPVALEGHDI
                     IGQAQTGTGKTAAFGCAIINNADFSGKKKSPKALILAPTRELAIQVNEELVRLGKHEK
                     LSVLPIYGGQPIDRQIRALKNGVDIVVGTPGRVLDLIRRKSLPLNDIGFLVLDEADEM
                     LNMGFIDDLEEIVKSLKTDRQTLLFSATMPPQIKKLARNYMKEDTKHIAIKKSSLTVS
                     KIEQFYFEIKHRDRFETLCRVLDFDEPNAAIIFCKTKKGVDEVVEKMQARGYMVEGMH
                     GDMSQNHRLQTLRKFKEGSLDFLVATDVAARGIDVESVTHVINYDLPQDNESYVHRIG
                     RTGRANREGVAYSLVTPKEYMMLKQIQKHTKSKIIRKAVPTVDEIFQNKHNNIINNVK
                     STIEEASEDFQKFIPIATQLDEDYSLVDVAAALLKIVFDKESSFDYTSNSIEAPVMDE
                     TRLFMSVGRRDGITPKSLVNFLKETARISPKQVGDIDIKENFCFANVDADAVNNIMKK
                     SMGKKLNKRTVNIEVAKKRK"
     gene            2048..2835
                     /locus_tag="K8M77_001460"
                     /pseudo
     CDS             2048..2835
                     /locus_tag="K8M77_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            2962..4248
                     /locus_tag="K8M77_001461"
     CDS             2962..4248
                     /locus_tag="K8M77_001461"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBI7085959.1"
                     /translation="MEAKMNKIDTNVVELEIKVDAKDFNEALKKSYNKNSKKFNIPGF
                     RKGKVPMNMVKKFYGVEVLFDDAINACIDKTYGVALEENNVRPVDYPQIEVVEVGEGK
                     DLVYKAKVTTYPEVTLGDYKGLEVEEVSYEVKDEDIEKQLADMQARNARVETKEEGTV
                     ENGNIAVIDFKGFIDDVAFEGGEGKDYPLEIGSGSFIDNFEEQLIGLKVGESKDVNVT
                     FPEAYGKEDLNGKPAKFEVTVKEIKVKELPALDDEFAKEVSEFDTLEELKADLKEKAV
                     KANELRAKREMEEKVINAVVDNAKVEIPEAMINREVENMVRDLEMRLGQQGLSLEQYY
                     EFTGSTEDKMKSYMKENAERKVKTDLVMSAVTEAEAIEATEEELKAKAEEVAKMYSNG
                     AETEKMVDLLLNAQRAALELDVKREKTLKMLFESLK"
     gene            4447..5031
                     /gene="clpP"
                     /locus_tag="K8M77_001462"
     CDS             4447..5031
                     /gene="clpP"
                     /locus_tag="K8M77_001462"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBI7085960.1"
                     /translation="MSNLVPMVVEQTSKGERSYDIFSRLLKDRIIMLSGEVNDVTANL
                     VVAQLLFLESEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGA
                     FLLSSGAKGKRFALPNAEIMIHQPLGGFQGQATDIDIHAKRILKIKDKLNQILSENTN
                     QPLEKIKVDVERDYFMEASEAVEYGLIDKVIERK"
     gene            5071..6378
                     /gene="clpX"
                     /locus_tag="K8M77_001463"
     CDS             5071..6378
                     /gene="clpX"
                     /locus_tag="K8M77_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBI7085961.1"
                     /translation="MGKFEDKKQLRCSFCGKNQEQVKRLIAGPGVYICDECIELCSEI
                     IEDEFEGNTESSPLETSNLPKPQEIKDYLDQYVVGQDRAKKSLAVAVYNHYKRINANK
                     AEDDVELQKSNILLLGPTGSGKTLLAQTLAKMLNVPFAIADATTLTEAGYVGEDVENI
                     LLKLIQNADYDVERAEKGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTT
                     ASVPPQGGRKHPHQEFIQINTSNILFICGGAFDGIDKIIEKRGTKSSIGFGAEVKGKS
                     EKNVGELLKDIMPGDLLKFGLIPEFVGRLPVLVTLEALDNEALVSILTKPKNALVKQY
                     KKLFELDNVKLDFTEEALKAIADEAINRKTGARGLRAIVEETMMDIMFDIPSEENIVK
                     ATITEETIKDRKDPELEKLPEGEVRPTLKTEKKNKARKDIETA"
     gene            6490..8202
                     /gene="lonB"
                     /locus_tag="K8M77_001464"
     CDS             6490..8202
                     /gene="lonB"
                     /locus_tag="K8M77_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HBI7085962.1"
                     /translation="MNTYTFIMFLQLLMSILFYIYMSKSFASKKKDNSVLEKENEKEM
                     EKLNKLRMIKLTEPLTEKSRPSNLEEIIGQEKGIKALKAALCGPNPQHVIIYGPPGVG
                     KTAAARIILEEAKKMAASPFNKDSKFVEIDATTLRFDERGIADPLIGSVHDPIYQGAG
                     SLGIAGVPQPKPGAVTKAHGGILFIDEIGELHPIELNKLLKVLEDRKVFLDSAYYSSE
                     DPNTPRYIKEIFDNGLPADFRLIGATTRSPEEIVPAIRSRCVEIFFRGLTVEEIRKIA
                     LNATNKVGYRISDEGLDIVSRYCTNGREVINLVQLCSGLAINENRDYIKENDIYWVIE
                     NGQYNPRMERMINDKPEIGYVNGLAVYGANNGALMEIEATAKLSSNSIGSIKITGIVD
                     DEELGGGEKKIKRKSTAYCSVQNVLTVLDNIFNLNSKAYDIHVNFPGGIPVDGPSAGI
                     SIATAIYSAIKGVPVNNRVAMTGEISIKGKVKPIGGVNAKILAAKRAGVELVIIPKEN
                     LGSITNEIDGIKIVGVKKIEEVLDLALYEEESIEKENLIIKDNRAFFGAGALNAESIK
                     KANT"
     gene            8324..10654
                     /gene="lon"
                     /locus_tag="K8M77_001465"
     CDS             8324..10654
                     /gene="lon"
                     /locus_tag="K8M77_001465"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBI7085963.1"
                     /translation="MKEDKLILPLIPLRGLTVFPNMVIYFDVGREKSIEAVEKAMAGD
                     QKIFLAAQKDIEIDNPSEEDIFNIGTICEIKQIVKMPKNTIRVLVEGIERAKMDEFFD
                     KEELLEASIEKIEIDNEIDHELEALSRKLKDDFFEFLDITANSGINGVDLFDNLEEEK
                     DLNKVTDLISSYALIKQEDKQDILQTLDLKQRIEKLIFYVKQEIEVAKIEKRIGTKVK
                     KKLDKGQREYYLREQMKVIQEELGEDDDNKKAIIEFEKLINEKKLPNQVKEKAQYEIS
                     KLKASSPYSQDGGVTRTYLENLLDMPWGEFTEDTLNIKDARKVLDKDHCGLKDVKDRI
                     LEYLAVKQISNSLRGPILCLVGPPGVGKTSIAKSVATSLNRKFVRMSLGGVRDEADIR
                     GHRRTYVGAIPGRIVTGLKEAKSMNPVFLLDEIDKLGMDFKGNPADALLEVFDNEQNK
                     TFRDHYLEVDVDLSEVMFITTANSLDGIPRPLLDRMELIEVSGYTYEEKFRIAKKYLV
                     PKVLKEHGVDNKIITISDSSLKLIIDSYTRESGVRNLQRQIANVIRKGIKDIIEKDKK
                     NLNISTKLVEKYLGPKIFSYEEIDKEDKVGVVTGMAWTSYGGDTLPVEVMVMDGKGRL
                     ELTGQLGDVMKESARAAYSYVRAHMKELGIKDEFYSKKDIHIHAPEGAVPKDGPSAGV
                     TMTTALVSALTGKKVKHNVAMTGEITLTGKVLAIGGLKEKCLAARRVGIDTVIVPKEN
                     EKDVIKLPKTVKDSLNIILVDKIDDVLENALVGVEK"
     gene            10654..11247
                     /locus_tag="K8M77_001466"
     CDS             10654..11247
                     /locus_tag="K8M77_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBI7085964.1"
                     /translation="MKIKSSEFIISAVKREQYPDDNLPEIAFVGRSNVGKSSIINSLT
                     NRRGLAKVSQTPGKTRLINFFLLNKDFYLVDLPGYGYAKVSKKEKASWGATIERYLLN
                     RGPLKKVVLLVDCRHKPTADDVQMYEWIKHYGYEVVVIATKSDKISNNQIGKSEKLIK
                     ETLGLPKDYKLKFFSSLNKKGKDELVDYLFDDLVEEI"
     gene            11463..11927
                     /locus_tag="K8M77_001467"
     CDS             11463..11927
                     /locus_tag="K8M77_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085965.1"
                     /translation="MDLNEILEGMSNDNCGNSGYCNTSGCGNSFDNCGQSVGGVNNFN
                     GFENNSCSGFGNNYGGFGNNCGGFGNNYGFGFGGGIWTLVWLFLLCGGGFGGNSNRCC
                     CYCEDECCCRRGNSGFGCNAIIWIIILSTLCGGFGNNCGNNNCNGLLGNCGC"
     gene            11963..12178
                     /locus_tag="K8M77_001468"
     CDS             11963..12178
                     /locus_tag="K8M77_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085966.1"
                     /translation="MSRKKKCCCNSHENYSRHGRCTGMDACTVMPTLLVLQCSGLLCN
                     DRAYILILLSLLCGGFNACGCNNNRCR"
     gene            12200..12451
                     /locus_tag="K8M77_001469"
     CDS             12200..12451
                     /locus_tag="K8M77_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085967.1"
                     /translation="MSRKKCCKCRCKCECKCKSKKHDCCSKQNRNTGCSGFNTCTTLP
                     TLLILCQSGLLCNDRAYILFLLFWLCGGVNANSCGYKCC"
     gene            12539..>13059
                     /locus_tag="K8M77_001470"
     CDS             12539..>13059
                     /locus_tag="K8M77_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085968.1"
                     /translation="MSKRRHKNKVSENSNQGNFMGGLNPQMLDMLGLGDVDINKVSEI
                     LSSMGSDGFDMNSINSILNNKDGSKNKSNKKGNMNNNNPLSAMMGNGNPMGDLSSIMG
                     LLGGGAQGMNQMGNMGNLGTIANMMGGNLGVPNNVSGDTSMDPIVTMILSLKNFVDPQ
                     RARFLDKVLKMYK"
BASE COUNT         5080 a         1463 c         2469 g         4047 t
ORIGIN      
        1 aaagctttgg tgaaaacgaa tttccactga aatacttaag tgaagaagaa ttagaagagt
       61 taggctttaa aaaaagttgc tgtggaagta gaggaggatg tcctaaaaaa gcaaatggaa
      121 cctgtggagg atgttcttca aagggaggat gccaaggagg atgttctaaa aatggatgtg
      181 gatgttcaaa tggatctggt tgttgtaaga ataaataaaa aaaatacata ctcatatgag
      241 tatgtatttt tttatattat tttctttttt tagcaacttc tatattaaca gttcttttgt
      301 taagtttttt acccattgat tttttcatta tattatttac tgcatcagca tcaacgttag
      361 caaagcagaa gttttctttt atgtctatat ctccaacttg cttagggctt attctagcag
      421 tctcttttaa gaagttaaca agagatttag gagttatacc atctcttctt ccaacagaca
      481 taaataatct agtttcgtcc attacaggag cttctataga atttgaagtg taatcaaaag
      541 aagattcttt atcaaatact atttttaaaa gagcagctgc aacatctact aagctataat
      601 cctcatctag ttgagtagct attggaataa acttttggaa atcttctgaa gcttcttcta
      661 tagtagattt tacattattt attatgttat tatgtttatt ttggaatatt tcatcaactg
      721 taggtacagc ttttcttatt attttactct tagtatgctt ttgtatttgc ttaagcatca
      781 tatattcttt tggagtaact aaagaatagg caactccttc tctattagct ctacctgttc
      841 ttccgattct atgtacataa ctttcattgt cttgtggtag atcgtagttt ataacgtgag
      901 ttacgctttc aacatctatt cctctagctg caacatcagt agcaactaag aaatctaagc
      961 ttccttcttt gaattttctt aaagtttgaa gtctatggtt ttggctcata tctccgtgca
     1021 taccttcaac catatatcct ctagcttgca ttttttctac aacttcgtca acaccttttt
     1081 ttgttttgca gaaaattatt gcagcatttg gttcatcaaa atctaaaact ctacaaagtg
     1141 tttcaaatct atctctatgt tttatttcaa aataaaattg ttctattttt gaaactgtta
     1201 aagaactttt ctttattgcg atatgcttag tatcttcttt catgtaattt ctagctaatt
     1261 tctttatttg tggaggcata gtagcagaga ataaaagtgt ttgtctatca gtttttaatg
     1321 atttaacaat ttcttctaaa tcatcgatga atcccatgtt taacatttcg tcagcttcat
     1381 ctaacactag gaatcctata tcgttaagag gtaaactctt tcttcttatt aaatctaaca
     1441 ctctaccagg tgttccaaca actatatcaa caccattttt taaagctcta atttgtctat
     1501 ctattggctg accaccatat attggtaaaa cagaaagttt ttcatgtttt ccaagtctta
     1561 caagttcttc attaacttgt atagcaagct ctctagttgg tgctaaaata agtgcttttg
     1621 gtgatttctt ctttcctgaa aaatcagcat tatttattat agcgcatcca aaagcagctg
     1681 ttttaccagt accagtttga gcttgaccga ttatatcgtg tccttctagg gctacaggaa
     1741 tactttcagc ttgtatttga gatggctctt caaatcccat atcttttata gctttaagta
     1801 agctttcctt aagacctaag tcatcaaatt ttatattgtt cattctttat cctctttctt
     1861 cttgtatatt tttagttttt aaaatttatt ttatttttaa aattctcaca atcaaacaaa
     1921 gcttatcata acatagtatc tccatataag caaatggtat tatttaaaat aaaaattaaa
     1981 aaagtttatt taaatgttta ctataagtta taataataga gattaaaaag gaaggagaga
     2041 aattaatttg gaagttggaa tttcttcagc atgtttttat ccagattttg aattggaaaa
     2101 atcatttaaa ataatgaaaa aattgggtta tgatacagga gaattatttt taaatactta
     2161 ttatgagtat agtgatgatt ttgtaaagaa attaattgag gaaaaagaga aaaataactt
     2221 taatataaaa tcagtacatt tttttcatct atgtatgaac cttatttatt tgatcaatat
     2281 gagagaagaa gaaaagattc tttagaagtt tttaggaata tatgtaaagc tactaataaa
     2341 ttaggagctt tttcatatac ctttcatggt atgagaaaac aagaatttaa tactattgat
     2401 aagaaggaaa ttttagatat atataatgaa ttagcttata tagcaagaga aaataatata
     2461 tatttatctc aagaaaatgt ttccaggtgt atgtcttcaa atatagaatt tttaaagtat
     2521 ttaaaaaata acataaaata tgatattaaa tttacctgtg atataaaaca agcatataaa
     2581 agcaatgaaa atcctctcca ttatattgat gtcatgggga gggatttaat taattttcat
     2641 ataaacgata gagatgaaaa aagtatttgt ttacttccag gtaagggaaa agtaaattat
     2701 aaacctatca ttgaaaaatt aaaaacttta gattataaag gaccattaat aatagaagtt
     2761 tatagggata attttaaaac ttacgaagaa ttaaaagatg ttaaagattt tatagaaaaa
     2821 aaactactag tatgataaaa tgtataaatg taagaataaa ataaaagaaa tatttgataa
     2881 aaaaagaaat tatatgttat aattgcctag gttaattcat aaaacatgaa tttaaaaggc
     2941 tatttacagg aggaatatac aatggaagct aaaatgaaca aaatagacac aaatgttgta
     3001 gaattagaaa ttaaagttga tgctaaggac tttaacgagg cgttaaaaaa gtcttataat
     3061 aaaaactcaa agaagtttaa cataccagga ttcagaaaag gaaaagtccc aatgaacatg
     3121 gttaaaaaat tctacggagt tgaagtttta tttgacgatg caataaacgc ttgtatagat
     3181 aaaacttacg gagttgcttt agaagaaaac aacgtaagac cagttgatta tccacaaatc
     3241 gaagtagtag aagttggaga aggaaaagac ttagtttaca aagctaaagt tactacttac
     3301 ccagaagtta ctttaggaga ttacaaagga ttagaagttg aagaagtatc ttacgaagta
     3361 aaagatgaag acatagaaaa acaattagct gatatgcaag caagaaatgc tagagttgaa
     3421 actaaagaag aaggaacagt tgaaaatgga aatatagctg ttatagactt caaaggattc
     3481 atagatgacg tagcattcga aggtggagaa ggaaaagatt atccattaga aataggttca
     3541 ggaagcttca tagataactt tgaagagcaa ttaattggat taaaagttgg agaaagcaaa
     3601 gatgttaacg taacattccc agaagcttat ggaaaagaag acttaaacgg aaaaccagct
     3661 aaattcgaag taactgtaaa agaaataaaa gttaaagaat taccagcttt agatgatgag
     3721 tttgctaaag aagtttcaga atttgatact ttagaagaat taaaagctga cttaaaagaa
     3781 aaagctgtaa aagctaacga attaagagct aaaagagaaa tggaagaaaa agttataaac
     3841 gctgtagttg ataacgcaaa agtagaaatt ccagaagcta tgataaacag agaagttgaa
     3901 aacatggtaa gagacttaga aatgagatta ggacaacaag gactaagctt agagcaatac
     3961 tatgagttca ctggaagtac tgaagataag atgaaatctt acatgaaaga aaatgctgaa
     4021 agaaaagtaa aaactgattt agttatgagt gctgtaactg aagcagaagc tatagaagct
     4081 actgaagaag aattaaaagc aaaagcagaa gaagttgcta aaatgtactc aaacggagca
     4141 gaaactgaga aaatggttga tctattatta aatgctcaaa gagctgcttt agagttagat
     4201 gttaagagag aaaaaacttt aaaaatgtta tttgaatctt taaaataata aataaataaa
     4261 taaataaact ttcagataat tgccaggaag tatttcttgg caattatttt aaaattaaaa
     4321 gtttgtttag aaaaagacta attaacaaat atctattttt tttatataat aattatgata
     4381 atgtatgttt ttatgatata tatggcaata ttataattag agtttttttt aaaaggaggg
     4441 aatttaatga gcaatttagt acctatggta gttgaacaaa caagcaaagg cgaaagaagt
     4501 tacgatatat tctcaagatt attaaaagat agaataataa tgttaagtgg agaggttaat
     4561 gatgtaacgg ctaaccttgt tgttgcacaa ttattattct tagaaagtga agatccagat
     4621 aaagatatac atctttacat aaattcccca ggaggttcta taacttctgg aatggcaatt
     4681 tatgatacta tgcaatatat aaagcctgac gtatctacaa tctgtatagg tatggctgct
     4741 tctatgggag cattcttatt atcttcagga gcaaaaggaa agagatttgc attaccaaat
     4801 gctgaaataa tgatacatca acctttaggt ggcttccaag ggcaagcaac tgatatagat
     4861 attcatgcta agagaatatt aaagataaaa gataaattaa accaaatctt aagtgaaaat
     4921 acaaatcaac ctcttgaaaa gataaaagta gatgttgaaa gagattattt catggaagca
     4981 tcagaagcag tagaatacgg attaatagat aaagttatag aaagaaaata atctacaact
     5041 taaatcttat tgaaagttga ggtggataag atgggaaaat ttgaagacaa aaagcaatta
     5101 agatgttctt tttgtggtaa aaatcaggaa caggttaaga gattaatcgc aggtcctggt
     5161 gtatatatat gtgatgaatg tatagagctt tgttcagaaa taatagaaga tgaatttgaa
     5221 ggaaatacag aatcatcacc attagaaact agcaatcttc caaagccaca agaaatcaaa
     5281 gactatttag atcaatatgt tgttggtcaa gatagagcta agaaatcttt agcagtagct
     5341 gtttataatc attacaagag aataaatgct aataaggcag aggatgatgt tgaactacaa
     5401 aaaagtaaca tacttttatt aggacctaca ggttcaggta agacattact tgcacaaact
     5461 cttgctaaaa tgttaaatgt tccatttgct atagcagatg ctacaacatt aacggaggca
     5521 ggatatgttg gtgaagatgt tgaaaatata cttttaaagc taattcaaaa tgcagattat
     5581 gatgttgaaa gagctgaaaa aggtataata tatatagatg agatagataa gattgctaga
     5641 aaatcagaaa atccatctat tacaagagat gtttctggag agggtgttca acaagcctta
     5701 ttaaaaatat tagagggaac aacagcttca gtaccaccac aaggtggaag aaagcatcca
     5761 catcaagagt ttatacaaat taacacttca aatattttat ttatttgtgg tggagctttt
     5821 gatggaatag ataaaataat agaaaaaaga ggaactaaga gttctatagg ttttggcgct
     5881 gaggttaaag gaaaaagtga aaagaacgtt ggagaacttt taaaggatat aatgcctggg
     5941 gatttattaa aatttggttt aataccagaa tttgttggta gattaccagt attagtaact
     6001 ttagaagctt tagataacga agctttagta agtattttaa ctaagccaaa gaatgctcta
     6061 gtaaaacaat acaaaaagtt atttgagctt gataatgtaa aactagattt tacagaggaa
     6121 gctttaaaag ctattgcaga tgaagctata aatagaaaaa ccggtgctag aggattaaga
     6181 gctatagtag aagaaactat gatggatata atgtttgata taccatcaga agagaatatt
     6241 gtaaaagcta ctataactga agagacaatc aaagatagaa aagatccaga gcttgaaaag
     6301 cttccagaag gagaagttag accaacttta aaaactgaga agaaaaataa agctagaaaa
     6361 gatatagaaa ctgcttaata gaaaaagatg catactattt atgcatcttt ttttgttttt
     6421 tatgaagttt ttggaatata atcacaaaaa taggtaaata ctaaccgtat agaacgggag
     6481 gtattagaga tgaatactta tacatttata atgtttctac aattacttat gtcaatttta
     6541 ttctatatat atatgagcaa gtcctttgcg agtaagaaga aagataatag tgttttagaa
     6601 aaagaaaatg aaaaagaaat ggaaaaatta aataaattaa gaatgataaa actgacagaa
     6661 cctttaactg aaaaaagtag accgagtaat ttagaagaaa taataggaca ggaaaaggga
     6721 ataaaagctc ttaaagcagc actttgtggg ccaaatccac agcatgtaat aatatatggt
     6781 ccaccaggag taggaaaaac tgcagcagct agaataattt tagaagaggc taagaaaatg
     6841 gcagcatctc cttttaataa ggattctaaa tttgttgaaa tagatgccac aactttaaga
     6901 tttgatgaga gggggatagc agatccatta ataggttccg ttcatgatcc aatatatcaa
     6961 ggagcaggtt ccttagggat tgcaggggtt cctcaaccta agccaggagc tgtaacaaag
     7021 gctcatggag gcatactttt tatagatgaa ataggagaac tccatcctat tgaattaaat
     7081 aaacttctta aagttttaga ggatagaaaa gtttttttag attcagccta ttatagttca
     7141 gaagatccca atactcctag atatataaaa gaaatatttg ataatggatt accagcagat
     7201 tttagattaa ttggtgcaac tacaagaagc ccagaggaaa tagtaccagc tataaggtca
     7261 aggtgcgtag aaatattttt tagagggcta actgttgaag agattagaaa aattgcttta
     7321 aatgctacaa ataaggttgg ctatagaata agtgatgaag gattagatat agtatctaga
     7381 tattgtacta atggaagaga agttataaat ttagtccaat tatgttctgg ccttgcaata
     7441 aatgaaaata gagattacat aaaagagaat gatatttatt gggttattga aaatggtcaa
     7501 tataatccta gaatggaaag aatgataaat gataaacctg aaattgggta tgtaaatggc
     7561 ttagccgtgt atggggctaa caatggagct ttaatggaaa tagaagctac agcaaagcta
     7621 tcaagtaata gtataggtag tataaaaatt actggaatag ttgatgatga ggaactaggc
     7681 ggtggagaga agaaaataaa gagaaaaagc acagcctatt gctctgtaca gaatgtattg
     7741 acagtattag ataatatatt taatttaaat tcaaaggcct atgatataca tgttaacttt
     7801 ccaggcggaa taccagtaga tggtccgtcg gctggaataa gtatagctac agctatatat
     7861 agtgccataa aaggagtgcc tgtaaataat agagtggcta tgactggtga gatatcaata
     7921 aagggaaaag taaaaccaat agggggagta aatgcaaaga tattagcagc caagagagca
     7981 ggagtagaat tggtgattat tccaaaggaa aatttaggta gtataactaa tgagattgat
     8041 ggaataaaga tagttggtgt taagaaaatt gaagaggtat tagatcttgc actttatgaa
     8101 gaagaaagta tagaaaaaga gaacttaata attaaagata atagggcatt ttttggtgct
     8161 ggtgccttaa atgcagaatc tataaagaaa gctaacactt aaagtgttag ttttcttttg
     8221 tgaaattaaa taagcttgaa aaaaacttgt aatatgtgta tacttgttaa agactatata
     8281 tttatataat atgtagttat taattttttt ggaggggata tttatgaaag aagataaatt
     8341 aattcttcct ttaattcctc taagaggtct aactgtattt cctaatatgg ttatatactt
     8401 tgacgttggt agggaaaagt caatagaagc tgttgaaaag gctatggcag gagatcaaaa
     8461 gatattttta gcagctcaaa aagatataga gattgacaat ccgagtgaag aggacatctt
     8521 taatataggt acaatctgtg aaataaagca aattgtaaaa atgccaaaaa acactataag
     8581 agttttagta gaaggtattg aaagagcaaa gatggatgaa ttctttgata aagaggaatt
     8641 acttgaagca tcaatagaga aaatagagat tgataatgaa attgaccatg aacttgaagc
     8701 tttatcaaga aagttaaagg atgatttttt tgagttttta gatattacag caaatagtgg
     8761 aataaatggt gttgatttat ttgataatct agaagaagaa aaagatttaa ataaggttac
     8821 agatttaatt tcttcatatg ctcttataaa acaagaggac aagcaggata ttcttcaaac
     8881 tttagattta aaacaaagga tagaaaaatt aatattctat gttaaacaag agatcgaagt
     8941 agctaagata gaaaaaagaa ttggaactaa agttaagaag aaattagata aaggtcaaag
     9001 agaatattat ttaagagagc aaatgaaggt aatccaagag gaacttggag aagatgatga
     9061 taataagaaa gctataattg agtttgaaaa attgataaat gagaaaaaat tacctaatca
     9121 ggttaaggaa aaagctcaat atgaaatttc aaagcttaag gcatcatctc catattctca
     9181 agatggagga gttacaagaa cttacttaga aaatttatta gacatgcctt ggggagaatt
     9241 tactgaggat actttaaata taaaagatgc gagaaaagta ttagataaag atcattgtgg
     9301 attaaaggac gtaaaggata gaattcttga atatttagct gtaaagcaaa taagtaattc
     9361 attaagaggc cctattcttt gcctagttgg acctccaggg gtaggtaaaa cttctatagc
     9421 caaaagtgtt gcaacttcac taaatagaaa atttgtaaga atgtctttag gtggagttag
     9481 agatgaagct gatataagag gacatagaag aacttacgtt ggagcaattc caggaagaat
     9541 agttactgga ttaaaggaag ctaaaagtat gaatccagta tttttactag atgaaattga
     9601 taagttagga atggatttca aaggaaatcc agctgatgcc ttactagagg tttttgataa
     9661 tgagcaaaat aaaactttta gagaccatta tttagaggtg gatgttgatt tatcagaagt
     9721 tatgtttata acaacagcaa actctttaga tggaattcca agacctcttt tagatagaat
     9781 ggaattaata gaggtatcag gatatactta tgaagaaaaa ttcagaatag ctaaaaaata
     9841 tttagtacca aaggtattaa aagaacatgg tgttgataat aaaataataa ctatatctga
     9901 ttcttcttta aaacttatta tagatagcta tacaagagaa tctggagtaa gaaatcttca
     9961 aagacaaata gctaatgtta taagaaaagg aataaaggat ataatagaaa aagataagaa
    10021 aaatttaaat atatctacta agttagttga aaaatactta ggaccaaaaa tctttagcta
    10081 tgaagaaatt gataaggaag ataaggttgg agtagttaca ggaatggctt ggacttctta
    10141 tggtggagat actttaccag tagaagtaat ggttatggat ggtaagggca gattagagct
    10201 tactggacaa cttggagatg taatgaaaga atctgcaaga gctgcatact cttatgtgag
    10261 agctcatatg aaggagttag gaataaagga tgagttttat tctaagaaag atattcatat
    10321 acatgctcca gagggtgcag taccaaagga tggtccttct gcaggggtta ctatgactac
    10381 tgccttagta tcagcattaa ctggtaaaaa agttaaacac aatgttgcta tgactggaga
    10441 aataacttta actggaaaag ttttagctat tggtggttta aaagaaaaat gcttagcagc
    10501 aagaagagtg ggaattgata cggttatagt tcctaaggaa aatgaaaaag acgtaattaa
    10561 acttcctaaa acagttaagg atagtttaaa tataatactt gtagataaaa ttgatgatgt
    10621 attagaaaat gcattggttg gagttgaaaa ataatgaaaa taaaaagttc ggaatttata
    10681 atatcagcgg ttaagagaga acagtaccct gatgataacc taccagaaat agcttttgtt
    10741 ggtagatcaa atgttggtaa atcttctata attaattcat taactaatag aagaggctta
    10801 gcaaaggtaa gtcaaactcc aggaaaaact agattaataa atttcttctt attaaataaa
    10861 gatttttatt tagtagattt acctggttat ggatatgcaa aagtatctaa aaaagagaag
    10921 gcatcttggg gtgctacaat agaaagatat ttattaaata gaggaccttt aaagaaggtt
    10981 gttttattag tggattgtag acataaacct actgctgatg atgttcaaat gtatgaatgg
    11041 ataaagcatt atggatatga ggttgtagtt atcgcaacaa agagtgataa gatatctaat
    11101 aaccaaatag gaaaaagcga aaagcttata aaagaaactt taggattacc taaagattac
    11161 aaacttaagt tcttctcttc attaaataaa aaaggaaaag atgaacttgt agattatctt
    11221 tttgatgatt tagttgaaga aatttaattt cataaattaa atataaaatt aactttttag
    11281 atgtttctag aatatattat taaagaagct gtgaagattt tatcttcgca gcttcttttt
    11341 tgaagtatat gcccttttgc taataattat tacaaaaaat agaaaatatt ccataaatat
    11401 gtgtcaaaaa ttcgtgaaga gaatactata tagtataaag aaaatatatg gaggtaacat
    11461 ttatggactt aaatgaaatc ttagaaggaa tgtcaaatga caattgtggt aactcaggat
    11521 actgtaatac ttcaggatgt ggaaattctt ttgataattg tggacaatct gtaggaggag
    11581 ttaataattt taacggattt gaaaataact cttgtagtgg atttggaaac aattatggtg
    11641 gctttggaaa taactgcgga ggattcggaa ataactatgg ttttggcttc ggtggcggaa
    11701 tatggacatt agtttggtta ttcttattat gtggtggagg attcggtgga aatagtaaca
    11761 gatgttgttg ctactgtgaa gatgaatgct gctgtagaag aggaaatagt ggatttggat
    11821 gtaacgctat aatatggata attattttat caactttatg tggtggcttc ggaaataatt
    11881 gcggaaacaa taactgtaat ggattattag gaaattgtgg atgttaagaa atgtttagaa
    11941 aataaaaaag gagtaaagag aaatgagtag aaagaaaaaa tgttgctgta atagtcatga
    12001 gaactatagc agacatggta gatgtacagg aatggatgct tgtacagtaa tgccaactct
    12061 tttagtttta caatgttcag gtttattatg taacgataga gcatatatct taattttatt
    12121 atcattacta tgtggtggat ttaatgcttg tggatgcaac aataatagat gtcgctaata
    12181 gaaaaaggag tgaattttta tgagtagaaa gaaatgctgc aaatgcagat gtaaatgcga
    12241 atgtaaatgc aaaagcaaaa aacatgattg ctgcagtaaa caaaatagaa atactggttg
    12301 ttcaggattt aatacttgca caacattacc aacattgcta atactttgtc aatcaggttt
    12361 attatgcaat gatagagcat atatcttatt cttattattc tggctatgtg gtggtgtaaa
    12421 cgcaaatagc tgcggctata aatgttgtta gaataatttt tggagggttt tttaaccctc
    12481 ctaaaaaaaa ataaaaaact atagtacata tacataaatt tattaaggag gataggagat
    12541 gtcaaagcgt aggcataaaa ataaagttag cgaaaattca aatcaaggaa attttatggg
    12601 aggattaaat cctcagatgt tagatatgtt aggacttgga gatgttgata taaataaagt
    12661 atcagaaatt ttatcttcta tggggagtga tggattcgac atgaattcta taaattccat
    12721 tttaaataac aaggatggaa gtaagaacaa aagtaacaag aaaggaaata tgaataataa
    12781 taatccttta tcagctatga tgggaaatgg aaaccctatg ggagatttaa gctcaattat
    12841 gggattacta ggtggcggag cacaaggaat gaatcaaatg ggtaacatgg ggaacttagg
    12901 aactattgct aatatgatgg gtggaaattt aggagtacca aataatgtga gtggtgatac
    12961 aagtatggat ccaatagtta ctatgatact ttcccttaag aattttgttg atcctcaaag
    13021 agctagattt ttagataagg ttttaaagat gtataagga
//
LOCUS       DAEVGI010000062        12885 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEVGI010000062 DAEVGI010000000
VERSION     DAEVGI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12885
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.062"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            150..1829
                     /locus_tag="K8M77_001471"
     CDS             150..1829
                     /locus_tag="K8M77_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBI7085969.1"
                     /translation="MKDFNFEEIIESCPMDYIYGKLTGDDYFDLFVLKSSIKEFNLKL
                     LSEVLGYNIDFKIIFSKNYNFIEEYCKGLNESFSLKLIDNDYVIIWFNRYLFKKYNKL
                     KLGFKKEKEILDLFLDSLKEYITFNHIDRKYVIEKTNKDIFFDDKNNEFKDISGIAEN
                     IFYKRAIEYGVNKNDEMFFEIFNYLDDVVIIKEGEKTIYVNNAFEKLYGLNCKDLYRE
                     NSMIVKLDRIHPKDRYKFNNIDFKKFFMAEARIIRADNEVRNVLFRANSIKNENGESI
                     RRIIIINDITNNIEKGNEMEKLRMEFFANISHEFKTPVNLIYSALQLLELKLKNNRDR
                     DGDIYINYIKMAKQNVFRLLKLINNLIDSTKLEAGFFNVNIKNHDIVSCVEDITMSIC
                     NFAEKNKISITFDTEEEKIIAFDFNHLERILLNVLSNAIKFNRENGNIDVYISFDDKY
                     ANISIKDTGIGIQKDKIDLLFYRFKKINNRLTKVNEGSGIGLYIAKELVKINGGDMIV
                     NSELGEGTEFIIKLPIKKNESKVLDEIALTSCEIENREELYKVELSDIYSI"
     gene            2046..3113
                     /locus_tag="K8M77_001472"
     CDS             2046..3113
                     /locus_tag="K8M77_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBI7085970.1"
                     /translation="MQILFGTLLLLLVLGGFTLFSYKAPHGMKAMGGLANAACASFLV
                     EAFHLAFFGEVFQIPFLAQVGSSNGSLGGVAAAILVPLALGVSPVYAVLTGLACSGFG
                     ILPGFIAGYLGSFVIKFLEKKIPAGLDLIVIIVLGAPLVRGIAAISNPLVETTLQNIG
                     GVITATSTASPIMMGIILGGIVTVVATAPLSSMALTAMLGLTGLPMAIGALAVFGSSF
                     MNFVFFGKMKFGSKKDTIAVAIEPLTQADIISANPIPVYVTNFIGGALSGIVVSLMGL
                     TNNTPGTATPIAGFAVMFAYNPAIKVLITAAIIIVLNVIAGMIGYLIFRNYKIMKADE
                     IRNNKDSENNLNVENEEVLVV"
     gene            3143..3823
                     /gene="sdaAB"
                     /locus_tag="K8M77_001473"
     CDS             3143..3823
                     /gene="sdaAB"
                     /locus_tag="K8M77_001473"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBI7085971.1"
                     /translation="MNNLCVFDILGPVMVGPSSSHTAGAARLGKVARAVAGEKIVDVK
                     FLLHGSFAKTYKGHGTDKALLAGILGMKPDDERLRSSLTIAKEEGLNFEFIESDLGDY
                     HPNTVKFVMKCESGKVCEVLGSSVGGGSIEIVEVNGNEANFSGAYPTLITCHDDKFGT
                     VNKVTKLLLEHEINIAFMNVSRTQKGMDATMTLEIDNKVDESVIKRMEEIDGIKKVIL
                     INLEEEEK"
     gene            3827..4705
                     /gene="sdaAA"
                     /locus_tag="K8M77_001474"
     CDS             3827..4705
                     /gene="sdaAA"
                     /locus_tag="K8M77_001474"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBI7085972.1"
                     /translation="MRVAKTGEELLDICKEEGIKLSEYALRCEMQSKNISKEEVLEKL
                     SKTLEVMKKSSSKGREEEVYSLSGLIGGDAFKLQKYLESGKSFTGNGAILAMAMAISS
                     SEVNASMGKIIACPTAGSCGILPAVMLTAKERLNLQDEDLLYGLLAASAIGLIIGKNA
                     TFSGAEGGCQAECGSASAMAAGGLVELMGGTPEMSLNAAAIVIKNILGLVCDPVAGLV
                     EIPCAKRNASGAVNAICAADLVMAGIESKIPFDDTVSAMAQVGKALPEILRETALGGV
                     AITKTGLMLNKKVFGK"
     gene            complement(4771..5505)
                     /locus_tag="K8M77_001475"
     CDS             complement(4771..5505)
                     /locus_tag="K8M77_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBI7085973.1"
                     /translation="MKTMQKLLSKTRQAIKEYNLIEDGDKIAVGLSGGKDSLTLLYIL
                     KNYQRFSPEKFELIAITLNPGGVDNSPLHELCKNIDVPFYEIQTDIKEIVFDIKKEKN
                     PCSLCANLRRGALNDNAEKLGCNKVALGHHKDDALETLMLSISYEGRINCFSPKSFMH
                     KNNLTLIRPMVFIEESQIRKAVAEYNFPVIKNPCPADGKTKREDMKNLIRSLDKTMPG
                     FKKNLFKSLTNTDQMFVWDKEIVKKM"
     gene            5712..6458
                     /gene="truA"
                     /locus_tag="K8M77_001476"
     CDS             5712..6458
                     /gene="truA"
                     /locus_tag="K8M77_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBI7085974.1"
                     /translation="MRNIKITLDYDGSKYKGWQKQNQKGSNVSTVQDKLEKVLTKMTS
                     EEIQLIGCGRTDSGVHAKNYVANFKTNSLMTLEQIIKYINEYLPEDIRVKEIREASER
                     FHARFNVKSKTYEYTIDNNKFKDVFLRKYAWHVEEKLDLEAMEEGAKYLLGTHDFKSF
                     TSLKSKNKSTLRTINSIEFHENNNILSIKINGNGFLLNMVRIIVGTLVDVGLGKFEPK
                     YINDILEAKERAKASEKAPAHGLCLLELNY"
     gene            complement(6525..7592)
                     /locus_tag="K8M77_001477"
     CDS             complement(6525..7592)
                     /locus_tag="K8M77_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBI7085975.1"
                     /translation="MNIKPLTIGNLTARLPIIQGGMGIGVSLSNLASAVTKNGGIGII
                     SGAQPGYLEEDFKNNHLEANLRALKKHIRIAKEKSQNGIIGVNLMVAMNNYAEHVKAA
                     IDSGVDLIISGAGLPSHLPKFTKGSNVKIAPIVSSLKAAKVILKLWDRHHKVSPDMIV
                     IEGPKAGGHLGFTKESLEDESKKFDSTILDIIKETSIYEDKYEKKIPIIVAGGVFDGK
                     DIAKYLKLGASGVQMSTRFVATYECDANMKFKEAYINCNKESISIVKSPVGMPGRAIR
                     NSFVQFTETKKSKVSHCYNCLIPCNPADTPYCISQALINAVKGDIDNGLIFCGENASK
                     IDKIVSVETLMNELEKDLLEA"
     gene            7792..8502
                     /locus_tag="K8M77_001478"
     CDS             7792..8502
                     /locus_tag="K8M77_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBI7085976.1"
                     /translation="MKKCDCKGYKSCLCNKELTIFKDLTEEEFNKLATGMTCFHFNKG
                     EYICYEGEKLSKLFLISKGSVKTSKLTKGGKEQIMHIFSVGDYFGETSLFNNEGGILF
                     NAIALNDVIVCAVDKDKVDSVLEENPRIAIVVLKSLTNRLKDTEEFAHKLVTNDVRVR
                     IAYMLKEFKRKYGKIENGEIFIDLPINREDMANYCGITRETMSRKLSIFEHEGLIEIR
                     GNKSLIIKDIERLNKIIS"
     gene            complement(8558..9469)
                     /locus_tag="K8M77_001479"
     CDS             complement(8558..9469)
                     /locus_tag="K8M77_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI7085977.1"
                     /translation="MKNKELLEECTLCTRNCKVNRFKSLGVCKASEKVKVARAELHPW
                     EEPPISFKNGSGTVFFSHCNLKCVFCQNHEISQDFKGLEISIERLVEIFLELQEKGAE
                     NINLVTPTHYAVQIIEALKIAKEKGLSIPILYNTNGYDSLETIKLLDGYIDVYLPDFK
                     YFKDDYALKYSKIKNYKDNLIPVLDEMFRQVGSPQFSEEGKLLKGMIIRHLMLPGLLF
                     DSKKVIDTIFSKFGDDVYISIMNQYTPMNKSCDYPEINKPLNHKHYESLIDYAASSGV
                     KNAFIQESGTNSKAYVPSFKYEGVMKK"
     gene            9614..9979
                     /locus_tag="K8M77_001480"
                     /pseudo
     CDS             9614..9979
                     /locus_tag="K8M77_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448399.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            10298..10969
                     /locus_tag="K8M77_001481"
     CDS             10298..10969
                     /locus_tag="K8M77_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085978.1"
                     /translation="MKEFLIVSILVSTLFLSEGLIYGSTFLEKEVDLNNTTSISLDKE
                     SYKDLYYVGEKASLTNEEKKVIENYALKYRERAIFLDLGANIDGFKEIGKERYYQITL
                     YSKSVKGNDLTGVVDRILINKKGNVLKGENGEPLSGIEGLITNLNISNNEIMELENIA
                     RNYEAKLNNKDLDVDIWGEKEINSQIYYGVNIYSNTLKKEGIFEPLEKLYINKSGEVI
                     RNTET"
     gene            10985..12661
                     /locus_tag="K8M77_001482"
     CDS             10985..12661
                     /locus_tag="K8M77_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085979.1"
                     /translation="MEYGDIKFLVRKSLNTEEGLNIRLKIKDVNLREIQLYRGKTKIN
                     NIKCKEEFYCDSNFIYINNKSRDLILEYEVLIGKLGKHGKGGEIEEDLISFMGEQILM
                     LPVEILIMNDDLRLNCILEIDFTDLIEEIKSEVYSEKDYKSIIPFKENDFKSKCVGGT
                     WSDLYEIMKSSYTFGFFEEIVLKKEYGEVHLYISIENKFLNDSSKAEVIRNIKSICDY
                     YYDLFKIDSLNKKDLNIVLLRKSKKENSYILGGSGKNVISATFDMNKKRDWQLLSHRI
                     FHAFMDDLLKSRVYHLPPNLWLTEGLATYYENLALESLEDGLKERLDIKFKKEMAILY
                     TRYLYMTLKEPSRFRIIPMEEGSIRSHGKIEFLHYTKAPLLVYFIESLKNSCGNKHEI
                     IEYLINNKDKSFSMQNLFYNLLDFRCDSFASKYLFENSIIPLWDLKEHLDDKEVICNL
                     QEYEYILWTWFLGEEENYIKDDLREYNKNIEEIISLRNINIYNSYLTKEIEDYSKELS
                     FLLKAWIIRSNICSVSSQDENIRYKLLKDKENLRIWKGFVQQSIKNKVNI"
BASE COUNT         4951 a         1339 c         2129 g         4466 t
ORIGIN      
        1 tatatttatt tatcatattt ttacataatt aaaataaaat taatatattg ttttaaagtt
       61 ccaaaatgaa tataataaaa taaaataagg gaaagagtgt tttaaactga aaataattta
      121 ttttcacaac ttaaagggga gttatattta tgaaagattt taattttgag gaaataatag
      181 agtcatgtcc tatggattat atttatggga aactaacagg tgatgattat tttgatttat
      241 ttgtgcttaa gtctagcata aaagaattta atttaaaatt gttaagcgaa gtactagggt
      301 ataatataga ttttaaaatt atatttagca aaaactataa ttttatagaa gaatattgca
      361 aaggtttaaa tgagagtttt agtttgaaat taattgataa tgattatgtg attatttggt
      421 ttaatagata cttatttaaa aaatataata aattaaaact tggatttaag aaagaaaaag
      481 aaatattaga tttattttta gattctttaa aagaatatat aacttttaat catattgaca
      541 gaaaatatgt aatagaaaaa actaataaag atatattttt tgatgataaa aataatgaat
      601 ttaaagatat aagtggaata gcagagaata tattctataa aagagctata gaatatggag
      661 ttaataaaaa tgatgagatg ttttttgaaa ttttcaatta cttagatgat gttgtaatta
      721 taaaagaagg agaaaaaact atttatgtaa ataatgcttt tgaaaaattg tatggtttaa
      781 attgcaaaga tttatataga gaaaatagta tgattgtaaa attagataga atccatccta
      841 aggatagata taagtttaat aatatagact ttaaaaaatt ttttatggca gaagcaagga
      901 ttattagagc agacaatgaa gttagaaatg ttttatttag agcaaattca ataaaaaatg
      961 aaaatggaga atctataagg agaattataa taataaatga tattacaaat aatatagaaa
     1021 aaggaaatga aatggagaaa cttagaatgg agttttttgc taatatatct catgaattta
     1081 aaacacctgt aaatttaata tacagtgcac ttcaactttt agaattaaaa ttaaaaaata
     1141 atagagatag agatggagat atttatatta attatattaa aatggctaag cagaatgttt
     1201 ttagactatt aaagctaata aataatttaa tagatagtac taagttagaa gctggctttt
     1261 ttaatgttaa tattaaaaat catgatatag taagttgtgt tgaagatata acaatgtcta
     1321 tatgtaattt tgctgaaaaa aataaaatat ctataacttt tgatacggaa gaagaaaaaa
     1381 taattgcttt tgatttcaat catttagaaa gaatactgtt aaatgtttta tctaatgcta
     1441 taaaatttaa cagggaaaat ggcaatatag atgtctatat tagttttgat gataagtatg
     1501 caaatataag tattaaagac acgggtatag gaatacaaaa ggataaaata gatttgcttt
     1561 tttatagatt taaaaaaata aataatagat taaccaaggt taatgaggga agtggaatag
     1621 ggctttacat agctaaggaa cttgtaaaaa ttaatggggg tgacatgata gttaatagtg
     1681 aacttggaga aggaactgaa tttataataa aacttccaat taaaaaaaat gaaagtaaag
     1741 ttttagatga gatagcctta acatcctgtg aaatagaaaa tagagaagaa ctttacaaag
     1801 tggagttatc agatatttat agcatttaga agaaaagctg tatcaattga tatagctttt
     1861 tttcatttta taagaagttt aaatttaaat atttatgtta tttattatac aaatttttaa
     1921 taagtaaaat aaattgtaaa ttatagcctg aaaacctttt ctatattagc gtgatattgt
     1981 aaaatgactt taccgataga atgattttat tattaaattt agaaaataca taggaggatt
     2041 ttattatgca aattttattt ggaacactat tattattatt agtattagga ggctttacat
     2101 tatttagtta taaggcgcca catggaatga aggctatggg tggattagca aatgctgcct
     2161 gtgcaagttt tttagttgaa gcctttcact tagcattttt tggagaagta tttcaaatac
     2221 cttttttagc acaagtaggt tcatctaatg gaagtcttgg cggagttgca gctgcaattt
     2281 tagtaccttt agcacttgga gtttctcctg tatatgcagt attaacaggt cttgcatgtt
     2341 caggatttgg aatattacca ggatttatag ctggttatct tggttcattt gttataaagt
     2401 ttttagagaa aaaaatacca gctggtcttg atttaatagt tattattgtc cttggagctc
     2461 cacttgtaag aggaatagct gcaatttcaa atccgcttgt agaaacaact ctacaaaata
     2521 ttggaggagt tataacagct acatcaacag ctagtcctat aatgatggga attatacttg
     2581 gaggaatagt aacagttgtt gcaactgcac ctttaagctc aatggcttta acagctatgt
     2641 taggattaac tggacttcca atggcaattg gagcattagc agtatttggt tcatcattta
     2701 tgaattttgt attctttgga aaaatgaaat ttggaagcaa aaaagatact attgcagttg
     2761 ctatagaacc tcttacacaa gcagatataa tttctgcaaa tccaatacca gtatacgtaa
     2821 ctaattttat agggggagct ttatcaggaa tagttgtttc attgatgggt cttacaaata
     2881 atacaccagg aactgctaca ccaatagctg gttttgctgt aatgtttgca tataatccag
     2941 ctattaaagt tttaattact gcggcaataa taatagttct taatgttatt gctggtatga
     3001 tagggtactt aatatttaga aattataaaa taatgaaagc agatgaaatt agaaataata
     3061 aagattctga aaacaattta aatgttgaaa atgaagaagt tttagtagtt taactaatag
     3121 ataaatagag gaagggatat ttatgaataa tctttgtgtt tttgatatat taggtccagt
     3181 aatggttggg ccatcatcat ctcatacagc gggagcagct agactaggaa aggtagctag
     3241 agcagtggct ggagaaaaga ttgtagatgt taaatttttg cttcatggat cttttgctaa
     3301 aacatataaa ggtcatggaa ctgataaggc tttacttgca ggtatattag gaatgaagcc
     3361 agatgatgaa aggcttagaa gctcattaac tattgctaag gaagaaggat taaactttga
     3421 atttatagaa agtgatttag gagattatca tccaaatacg gttaaatttg ttatgaagtg
     3481 tgaatctgga aaagtttgtg aggtgttagg ttcatcagta ggtggaggaa gcatagaaat
     3541 agttgaggta aatggaaatg aagctaattt tagtggagcc tatccaacac ttattacatg
     3601 tcatgatgat aaatttggta ctgtaaataa agtaacaaaa cttcttcttg aacatgaaat
     3661 taatattgct tttatgaatg tttcaagaac tcaaaaggga atggatgcta ccatgacttt
     3721 agaaatagat aataaggttg atgaatctgt tataaagaga atggaagaaa tagatggaat
     3781 aaaaaaagtt atacttataa acttagaaga agaggagaaa taacatatga gagttgcaaa
     3841 aactggagaa gaacttttag atatatgtaa agaagaaggg attaaactta gtgaatatgc
     3901 cttaagatgt gagatgcaaa gtaaaaatat ttcaaaagaa gaagttttag agaaactttc
     3961 aaaaacttta gaggttatga aaaaatcatc atcaaaagga agagaagaag aagtatattc
     4021 acttagtgga ttaataggag gagatgcttt taagcttcaa aagtatttag aatctggaaa
     4081 atcttttaca ggtaatggag ctatattagc tatggctatg gctatttctt catctgaagt
     4141 aaatgcttct atgggaaaaa taattgcttg tccaacagcg gggtcttgtg gaattcttcc
     4201 tgcggtaatg ttaacagcta aggaaagatt aaatcttcaa gatgaagatt tattatatgg
     4261 gcttttagca gcatcagcta ttgggcttat aattggaaaa aatgctacat tttcaggagc
     4321 agaaggtgga tgtcaagcag aatgtggctc agcatctgct atggcagctg gtggccttgt
     4381 agaacttatg ggaggaacac cagaaatgtc attaaatgct gctgcaatag taataaagaa
     4441 tatattaggt cttgtttgcg atcctgttgc aggtcttgtt gaaattcctt gtgctaaaag
     4501 aaatgcttca ggtgccgtaa atgctatatg tgcagcagat cttgtaatgg ctggtataga
     4561 atcaaaaata ccttttgatg atacagtaag tgctatggct caagttggaa aagctcttcc
     4621 agagatctta agagaaacag cacttggagg agtagctatt acaaaaacag gtcttatgtt
     4681 gaataaaaaa gtattcggta agtaatttta ataatataaa tttatattat gtatttataa
     4741 aaagtgtaca agttaatact tgtacacttt ttacattttc tttactattt ctttatccca
     4801 tacaaacatt tgatcagtat tagttaaaga tttaaataaa tttttcttaa aaccaggcat
     4861 agttttgtct aaacttctaa taagattttt catatcttct cttttagttt taccatctgc
     4921 aggacaagga ttttttatta ctgggaaatt atattctgct actgcctttc ttatttgact
     4981 ttcctcaata aacaccatag gtcttataag agttaaatta tttttatgca taaagctttt
     5041 aggagagaag cagtttattc ttccttcata acttatggag agcataaggg tttctaaggc
     5101 atcatcttta tggtgtccta aggcaacttt gttgcaacca agcttttcag cattatcatt
     5161 taatgcacct cttcttagat ttgcacataa tgagcatgga tttttttctt tctttatatc
     5221 aaaaactatt tcctttatat ctgtttgaat ttcataaaaa ggaacatcta tatttttaca
     5281 aagctcatgt aatggactgt tatcaactcc tccagggttt aaagttatag ctattaattc
     5341 aaatttttca ggagaaaatc tttgatagtt ttttaatata tataaaagtg ttagactatc
     5401 ctttccacca gaaagtccta cagctatttt atcaccatct tcaataaggt tatattcttt
     5461 tatagcttgt ctagttttac ttaataattt ttgcatggtt ttcataaaaa tcacctctta
     5521 atctttattt aattatctta taataaaaat gtgaaaatta aaagagttaa aattttagaa
     5581 taaaattaaa tttattgtca gatatttaaa ttgatattcc aatttaaaca tgttacatta
     5641 aaacttagat taagtattaa aattagaaat ttaaatttat atatttaaat tatttttgaa
     5701 aggaaattaa aatgagaaat ataaagatta ctttagatta tgatgggagt aagtacaagg
     5761 gatggcaaaa gcaaaaccaa aagggaagta atgtttctac agttcaagat aaattagaaa
     5821 aagtactaac taaaatgact agtgaagaga ttcaactaat tggttgtgga agaactgata
     5881 gtggtgtaca tgcaaaaaat tatgtagcaa atttcaagac aaattctctt atgactctag
     5941 aacaaattat taaatacata aatgaatatt tacctgagga tataagggta aaggaaataa
     6001 gagaagcaag tgaaagattt catgctagat ttaatgttaa gtctaaaaca tatgaatata
     6061 ccattgataa caataagttt aaagatgtat ttttaagaaa atatgcatgg cacgttgaag
     6121 aaaagttaga cctagaagct atggaagaag gagcaaaata tttattaggc actcatgact
     6181 ttaagagttt tacaagttta aaatcaaaga acaaatcaac tttaagaact ataaatagca
     6241 ttgaatttca tgaaaataat aatatattaa gtataaagat aaatggaaat ggcttcttat
     6301 taaatatggt tagaataata gtaggaactt tagtagatgt aggacttgga aaatttgaac
     6361 ctaaatatat aaatgatata cttgaagcta aagaaagagc aaaagcttca gaaaaagcgc
     6421 cagctcatgg actttgtctt ttagaattaa attattaaat aaaatctgta taaaagagta
     6481 taaatactaa ttaatagttt aactactctt ttatacagat tattttatgc ttctaataaa
     6541 tctttttcta attcattcat aagtgtctct acagatacta ttttatctat ttttgaggcg
     6601 ttttcaccac aaaaaataag tccattatct atatcgcctt ttacagcatt tataagtgct
     6661 tgacttatgc aataaggagt atcagcagga ttacaaggga ttaggcaatt ataacaatga
     6721 gaaaccttag atttttttgt ttctgtaaat tgaacaaagg agttcctaat agctcttcct
     6781 ggcattccaa caggactttt tactatggat atactttctt tattacaatt tatatatgct
     6841 tctttaaact tcatatttgc atcacattca taagttgcca caaaacgagt tgacatttga
     6901 actccacttg cacctagttt taaatatttt gcaatatcct ttccatcaaa aactccacct
     6961 gcaactatta ttggaatttt cttttcatat ttatcttcat aaatagaagt ttcttttatt
     7021 atatctagta ttgttgaatc aaacttttta ctttcatctt caagtgattc tttagtaaat
     7081 cctaaatgtc ctccagcttt tggtccttca attacaatca tatctggaga tactttatga
     7141 tgcctatccc aaagttttaa aattactttg gcagctttaa gggaagaaac tataggagct
     7201 atttttacat tgctaccttt tgtaaattta ggaagatgag agggaaggcc agctcctgaa
     7261 attattaagt caactccaga gtctatagca gcctttacat gctctgcgta gttattcata
     7321 gctaccatta aattaactcc tataattcca ttttgtgatt tttctttagc tattcttata
     7381 tgttttttta aagctcttaa attagcttct aaatgattat ttttaaagtc ttcttctaag
     7441 tatccaggtt gagcaccaga tataattcct attcctccat tttttgttac agcactagct
     7501 aaatttgaaa gggacactcc aattcccatt cctccttgta taataggtaa tctagctgtt
     7561 aaatttccaa ttgtaagtgg ttttatattc atataaacta tcctttcttt aaaaaattag
     7621 aaaaacaaat attttgatta ttaaaatatt tgaatataaa tatattataa ggataagtta
     7681 agaaataaac aagtattaaa ataaattaat taatagtaaa aaaatatgaa taaatatatt
     7741 ataattaata ctaaagatat aggattataa ataagaagtt aggagagagc tatgaaaaaa
     7801 tgtgattgca agggctataa aagttgttta tgcaacaaag agcttactat atttaaggat
     7861 ttaacagagg aagaatttaa taaattagct actggaatga catgttttca ttttaataaa
     7921 ggggaatata tatgttatga aggtgaaaag ctttcgaagc tatttctaat aagcaaggga
     7981 agtgtaaaga catcaaagct tactaaggga ggaaaagagc aaataatgca catattctct
     8041 gtaggggact attttggaga gacaagccta tttaataatg agggaggaat tttatttaat
     8101 gctattgctt taaatgatgt tatagtttgt gctgtagata aggataaagt tgatagtgtc
     8161 ttagaagaaa atccaagaat agctatagtt gttttgaaat ccttaactaa tcgtttaaag
     8221 gacactgaag aatttgctca taaattagta actaatgatg ttagagttag aatagcatat
     8281 atgttaaaag agtttaagag aaaatatggc aaaatagaaa atggggaaat atttattgat
     8341 ttaccaatta atagagaaga tatggctaat tattgtggta taactagaga aacaatgagt
     8401 agaaaactct ctatatttga gcacgaagga ttaatagaaa ttagaggaaa taaatcttta
     8461 ataataaaag acatagaaag attaaataaa ataatttcat aggaaacact tattcctatg
     8521 aaattatttt atttttagaa aaaatctatt taaaagttta ttttttcata actccttcat
     8581 atttaaatga gggaacatag gcctttgagt tagttccact ttcttgtata aatgcatttt
     8641 taactcctga agatgctgca taatctataa gagattcata atgtttatgg tttaaaggct
     8701 tatttatttc aggataatca caagacttat tcataggagt atattgattc ataatgctta
     8761 tataaacatc atctccaaat ttagaaaata tagtgtctat aactttcttt gaatcaaata
     8821 aaagacctgg aagcattaaa tgtctaatta tcataccctt taaaagttta ccttcctctg
     8881 aaaattgtgg agaacctacc tgcctaaaca tttcatctaa tactggaatt aaattgtcct
     8941 tataattttt tatctttgaa tatttaagtg cataatcatc cttaaaatat ttaaaatcag
     9001 gaagatatac atctatgtaa ccatctaata actttatagt ttctaaacta tcatatccat
     9061 tagtattata gagaatagga atacttaagc ctttttcttt tgctattttt aaagcttcta
     9121 ttatttgaac tgcataatgt gttggagtaa ctaagtttat attttcagct cccttttctt
     9181 gaagctctaa aaagatttct actaaccttt caattgatat ttcaagtcct ttaaaatctt
     9241 gacttatttc atggttttgg caaaaaacac attttaaatt acaatgagaa aaaaatactg
     9301 ttccagatcc attcttaaag gaaataggag gttcttccca aggatgaagt tctgctctag
     9361 ctactttaac tttttcacta gccttacata ccccaagaga tttaaatcta ttaactttgc
     9421 agtttctagt gcaaagagta cattcttcta atagttcttt atttttcatt aaaacacctc
     9481 taatctatat tcaatattaa ttatatattt taaggaatta agtaacaaga ttcttaaaat
     9541 agaaatagtg atttatggca ttatatggtc tatatgttat aattaatata taaatagagg
     9601 tggtgaaata tatggacatg gagcatcata tttgctagag gatataacta aatttatagt
     9661 agtagatatg gggtgtattg gtgatgactt aagttgcaca gagtatgatg tttctatttg
     9721 tgcaaaggat tcaggtggac cttatgacta taaaattaca tcagatctta tagaattagc
     9781 taaaaacaat aatttaagct atgcagtaga catataccct agatatggct cagatgttgg
     9841 agctgcctta cgaggtggaa ataacataaa aggtgggctt ataggccctt gagttcacgc
     9901 tttgcatggt atggagagaa ctcactatga tgctttagaa aataccataa aactaatata
     9961 tttatattta acaaggtaaa ataagaaaaa taattaacaa atttgagtca tctaaaagta
    10021 aaaaatactt ttaggtgact ttttttaggt tattatataa ctatttatat atttcactta
    10081 aaaatataag atatgaacta cataattaat aattttttta cttgaattaa gggttagcta
    10141 atattaaaat taataaaact aaaataatta atttgaattt aactttgaat tttccttaga
    10201 taatattagg attaaatgaa actacattat aaggtgatta ataaatagaa cttttgtaaa
    10261 taatatactt aagataaagt tgattggagt gtagaggatg aaagagtttt taatagtaag
    10321 tattttagtt tctactttat tcttaagtga aggattaatt tatggaagta cttttttaga
    10381 aaaagaagtt gatttaaata atactacaag tatttcatta gacaaagaat cctataaaga
    10441 tttgtattat gttggggaaa aagcctcttt aactaatgaa gaaaaaaagg taatagagaa
    10501 ttatgcttta aaatatagag aaagggctat tttcttagat ttaggcgcta atatagatgg
    10561 atttaaagaa ataggaaagg aaagatatta tcaaataacc ttatattcta aaagtgtaaa
    10621 aggtaatgat ttaacgggag tagtagatag aattcttata aataaaaagg gaaatgtttt
    10681 aaagggtgaa aatggggaac ctttaagtgg aatagagggt ctcataacaa atttgaatat
    10741 aagcaataat gagattatgg agttagagaa tattgcaaga aattatgaag caaaattaaa
    10801 taataaagat ttagatgtag acatatgggg agaaaaagaa attaatagtc agatttatta
    10861 tggagttaat atatactcta atactttgaa aaaagaagga atttttgaac ctttagaaaa
    10921 attatatata aataaatctg gagaagttat tagaaataca gagacttaga ttaggagggt
    10981 ataaatggaa tatggagata ttaaattttt agttaggaaa agtttaaata cagaagaggg
    11041 actaaatata aggcttaaga ttaaagatgt taatttaaga gaaattcagc tttatagggg
    11101 taaaacaaaa ataaacaata taaagtgtaa agaggagttt tattgcgatt caaacttcat
    11161 atatattaat aataaaagta gggatttgat tttagaatat gaggttttaa taggtaaatt
    11221 aggaaagcat ggaaaaggtg gagaaataga agaggattta ataagcttta tgggtgaaca
    11281 aatacttatg ttaccagttg aaattcttat aatgaatgat gatctaagat taaattgtat
    11341 cctagaaata gattttaccg atcttataga agagataaag agtgaagtct atagtgaaaa
    11401 ggattataaa agtataatac cttttaaaga aaatgatttt aagtcaaagt gtgtaggggg
    11461 gacttggagt gacctatatg aaataatgaa gtcttcatat acctttggct tttttgaaga
    11521 aattgtttta aagaaagaat atggagaagt acatttatat attagtattg aaaataaatt
    11581 tttaaatgac agtagtaagg cagaggttat aagaaatata aagtctattt gtgattatta
    11641 ttatgattta tttaaaatag attctctaaa taagaaagat ttgaatatag ttttacttag
    11701 aaagagcaaa aaggagaact cttatatatt aggcggaagt ggaaaaaatg ttataagtgc
    11761 tacttttgat atgaataaaa agagagattg gcaattatta tcccatagaa tttttcatgc
    11821 ctttatggat gatcttttaa aatcaagagt ttatcatctt cctccaaatc tttggttaac
    11881 agaggggtta gctacttatt atgaaaactt agctttagag tccttagaag atggattaaa
    11941 agaaagacta gatataaagt ttaaaaagga aatggcaatt ttatatacta gatatttata
    12001 tatgacttta aaagagccaa gtagatttag aataattcct atggaagagg gaagcataag
    12061 aagtcatggt aaaatagagt ttttacatta tacaaaggct cccttattag tatattttat
    12121 agaaagttta aagaattcat gtggtaataa acatgaaata atagaatatt taattaataa
    12181 caaagacaaa agtttttcta tgcaaaatct tttttataac ttattagatt ttaggtgtga
    12241 tagctttgca tctaaatatt tatttgaaaa tagtataatt cctctatggg acttaaaaga
    12301 acatttagat gataaagagg ttatttgtaa cttacaggaa tatgagtata ttttgtggac
    12361 atggttttta ggagaagaag aaaattatat taaagatgat cttagggaat ataacaaaaa
    12421 tattgaggaa atcataagcc ttagaaatat taatatttat aattcttatt taacaaagga
    12481 aattgaggat tattctaagg aattaagctt tttattaaag gcttggatta ttagaagtaa
    12541 tatatgtagt gtttcttctc aagatgaaaa tattagatat aaacttttaa aggataaaga
    12601 gaatcttaga atttggaagg gatttgtaca gcaatccata aaaaataagg tgaatatata
    12661 agataaggca tctattaatt ttatgaaggt tataatatga tttttttagt tataatataa
    12721 aatatttaat aaatcaacat aatatataaa gctaacaaaa taatataaat gagcctttag
    12781 taaagttaat attattatat taactttata ataaatacat tataagctta tttattgtgt
    12841 ctaaatataa aaatatataa agataaaagt taaatatcaa taatt
//
LOCUS       DAEVGI010000063        12859 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEVGI010000063 DAEVGI010000000
VERSION     DAEVGI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12859
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.063"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            193..606
                     /locus_tag="K8M77_001483"
     CDS             193..606
                     /locus_tag="K8M77_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085980.1"
                     /translation="MSLCKGTVKIGKKNLKRGTNKYFFKRYLKKNYEFTVSEFKDRNK
                     FELLEPVNVEGLDFLVDVEFRGIRPKEIDLRSVDNLEKYQFERYEQWVSNNLGISLEN
                     SKWKNKKFNWGNLNVKLSKTVKEDLGCITIKVVYK"
     gene            1088..1786
                     /locus_tag="K8M77_001484"
     CDS             1088..1786
                     /locus_tag="K8M77_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBI7085981.1"
                     /translation="MEENTISLQEIAYALKKRWKLIALITIAATLVSAILSFFVIKPQ
                     YEAKTKLFIGKQETSQNAANYDNNDIMMYQKLMKTYAELAKTSDLVTKAVKNAKLDYN
                     QNEIKGVLNNLNVTPSADTQILDLSFKGKNPKEVLKVTEAITDEFISESKELIPNGNV
                     QVIQKPQLPENPVSPNKKLNILIAFVLGLMIGVGIVLLLEYLDNTFKSREELEKTLDL
                     PIIGAIPDYDSIEK"
     gene            1799..2452
                     /locus_tag="K8M77_001485"
     CDS             1799..2452
                     /locus_tag="K8M77_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBI7085982.1"
                     /translation="MLILEKNPKSVAAESYRTLRTNIQYSSFDNPVKSIVITSSEPGE
                     GKSTTSANLALSFAQDGKKVILIDCDLRKPVVHKEFQISNSRGLSEFLIGNVEFSKVV
                     YKHESGLHVLPSGLVPPNPAEMLASRSMEHLLTQLEEKYDYIILDTPPVNAVTDSKIL
                     STKVDGTILVVKYGYTKKDGVIEAVKGLRAVKANIIGTVFNGEENQRGKYYHYYKKD"
     gene            2628..4538
                     /locus_tag="K8M77_001486"
     CDS             2628..4538
                     /locus_tag="K8M77_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBI7085983.1"
                     /translation="MSKISKVKGILLVLIDILLINIAYIFSMLVRFAFNGPVLGDIPR
                     EQWSLYFKWMPLIAVIYVVLFTFFKMYKSLWSFAGLDEVMKGVAACVLGCLINVIIML
                     FLNPRIPMMVTLSAGILTMVLTLGFRLSFRMYRRLVIYGTLKGIESQKNVLIVGAGSC
                     GRLVINEMKRETGAGFKPIGVIDDDRSKFGTYINGVKVLGNRNDIKNIVENQRVDLIL
                     IAIASITSDEKKKIIEACHETKVKVKIMPGVYEMIGGKVNLTKMRDVDLRDLLGREEV
                     KLSKDEIANYIKNKRVLVTGGGGSIGSELCRQIANFNPKELMILDIYENNAYDLEMEL
                     NRTHPELKKQVIIASVRDKEKLDSIFRSFKPQVVFHAAAHKHVPLMETSPGEAIKNNA
                     GGTLNTAECADKYGVEKFVLISTDKAVNPTNVMGATKRMCEMIIQAINKKSKTDFVAV
                     RFGNVLGSNGSVIPLFKKQIAEGGPVTLTNKYITRYFMLIPEAAQLVLQAGAYAKGGE
                     IFVLDMGKPVKIYDLAKNLIKLSGYEPFEDIDIKIVGLRPGEKLYEELLMDEEGLTET
                     KHEKIFIGKPGDFDFNYIKSNIEELINISRFGTGDDIRDKIEEFVPTYNRLDKKDKNE
                     VESSKLEVATTL"
     gene            4606..5712
                     /locus_tag="K8M77_001487"
     CDS             4606..5712
                     /locus_tag="K8M77_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI7085984.1"
                     /translation="MGGLEEVFVKEAFDTNWIAPLGANVDGFEKELSEYVGSKTGAAL
                     ASGTAAIHMALKAVGVEKGDKVFCSSLTFAASCNPIIYEGGIPIFIDSEPESHNMSPV
                     ALEKAFKVYEEKGEIPKAVIVVNLYGQSADMDKIIEICKKYNTPIIEDAAESLGATYK
                     GKHSGTFGDYGIYSFNGNKIITTSGGGMLVSNNEEGIAKVRFWSTQARDKARHYEHTE
                     LGYNYRMSNIVAGIGRGQLRVLEDRIAKKKEIFETYKEAFKDIEDIEMMPVCEYGEPN
                     YWLTTITLNENSKVKPLDIILALEKENIESRPIWKPMHIQPYYKEYDFYSHNDEEEMS
                     VSEDIFNRGVCLPSDTKMTDKEQKRVIEIIKNIF"
     gene            5727..6269
                     /locus_tag="K8M77_001488"
     CDS             5727..6269
                     /locus_tag="K8M77_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7085985.1"
                     /translation="MNLYGDKIKLRALEPDDMEFLRETVNNPEIEKLVGGWSFPISKK
                     QQMDWYEKIINDKTSLRFAIETDEDGIIGMADLRNIDWKNRVAFHGIKIGNKEYRGKG
                     YGKDVVFTIMKYAFEELQLNRLEVTILESNIASQKLYVDKCGLVVEGVRRNCVFKNNK
                     YHNQLVLGILREEYFNLKNK"
     gene            6281..6925
                     /locus_tag="K8M77_001489"
     CDS             6281..6925
                     /locus_tag="K8M77_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBI7085986.1"
                     /translation="MYKNYLKRVLDVIFASVGIVVFFPIYILVAVMVRINLGSPIIFK
                     QKRPGKDEKIFTMYKFRSMSDQKDKNGNLLSDKDRLTKFGRVLRSTSLDELPELFNIL
                     KGDMSIVGPRPLAIVYLEYYNEEEKKRHSVRPGLTGLAQINGRNLSNWDERFKNDIEY
                     VENISFTNDFKIILKTIKKVLLKEDVVARGEGKTLDFHIYRQKQLEENNKLKRY"
     gene            6926..8017
                     /locus_tag="K8M77_001490"
     CDS             6926..8017
                     /locus_tag="K8M77_001490"
                     /inference="COORDINATES: protein motif:HMM:NF013227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBI7085987.1"
                     /translation="MKREIGSFFWEYKIDNTENNLSFFENLGKDKLFLSSGRDALKKL
                     IKEIGKNKTILLPGFTCSSVIIPFVNNGYKVHFYDLENNLEVDVELFLRKIKEVSPDI
                     ILIHSYFGFDTIKNIRPLLKDIRKNNIIVVEDITQRIYSKFDFVQSDYIVSSLRKWFA
                     IPDGGVLINRLNDLKLKEDKKINNKLYDLSLKMYDIKKQYIENGDLILKEKYYEIFKK
                     TKVEFELWNEIYSMSNRSIEIYNSVNLDFINYKRYENYKYLIENLINLEGIEIIFKSI
                     DKNIVPLYFPIFIKNGKRDEIQRKMAKRNIYCPIVWPMSKFVYRKINSKTINIYNEIL
                     CIPCDQRYDIDEIKFVVDNLKIVINNSIL"
     gene            8047..9036
                     /locus_tag="K8M77_001491"
     CDS             8047..9036
                     /locus_tag="K8M77_001491"
                     /inference="COORDINATES: protein motif:HMM:NF016242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBI7085988.1"
                     /translation="MVKLEKDIYFEENYSKLYEDIENGKTKVFRLNSEYGEIENIFIK
                     RKIDFYKKEEIYDITTPYGYGGPRIVWVDNDNKDKLIKEYEKKFKKFCKKENIIAEFI
                     RFNPIIGNHEYFKDIYDVKNIRKTLATNLKDYKDPIKEEFSKSARKTIRQVLKKGVTY
                     KVIQCPNELEEFKKIYYSTMDRKNADDYYYFDDEYFNKCLEFFKENIISIQVIFEEKI
                     IAASLYFVYNNILEAHLSGTLSEYLYMSPAYIIKYATALWAKENNIDIIHYGGGTSNN
                     IDDPLYTFKKKFSQKTELDFYIGKKIWNLNLYKEICDLKNVNYEDEYFPAYRK"
     gene            9075..10295
                     /locus_tag="K8M77_001492"
     CDS             9075..10295
                     /locus_tag="K8M77_001492"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI7085989.1"
                     /translation="MKKDILMITHFTQVPWEAGNGRFLYLLNMFDYEKVKVELVTTDF
                     SHRTKEKRKKNNENLRYKLTYVEELGYKKNVSIKRFFSHYIFAKNLRKYLVSRKKPDV
                     IYCSIPSTDAAYYVTKYAKKNNIKLIIDIQDLWPEAFKMVFNIPIISDIIFYPMNRKA
                     NFVYKNADKIIGVSETYVNRALSVNRKVEKGLSVFLGTDLNYFDSLIKNDEKYNDFTI
                     VYVGTLGHSYNIKLIIDALEQVKLKYNKNINFLIMGDGPLKNEFQSYSKNKNLQIEFT
                     GRLKYEDMVYRLKKCHLAVNPIVDGSAGSIINKVGDYAAAGLPVINTQTCVEYKELIE
                     KYNLGFSCLNNDIDGIANKIFELYKDENLRKKFGANNRMVAEILFDRGKTYKKIINII
                     MGEEDENITYCINN"
     gene            10264..11358
                     /locus_tag="K8M77_001493"
     CDS             10264..11358
                     /locus_tag="K8M77_001493"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI7085990.1"
                     /translation="MKILHIASITDNKASGMSVAIPKHVKFQGYYAETALLNLNLNQL
                     EEHSIRIFNLNNLNRQGISNLPNNFSRPDLVVLHGIYYIKFLTIYRYLRKNKIPYVLV
                     PHGCLSKYAINKKKIKKKLALKLLFNKIINNCVSIQYLSEIEKEISFGDSKKNFISPN
                     GIEINFNNKIYNLGRELNLIYIGRISILEKGLDLMIEAINLIQNIMRENNAKLNIYGS
                     DVDDGKNILLSMCKKYKIEDIVKINDAIFDKNKIEVINNSDIFIQTSRTEGQPMGILE
                     AMSYGLPIIATKGTSFADDIKENLCGWSCENNIEDIAMAIKKACDEKKYIECYGKNSR
                     EFVNRKYSWDIISREAIEEYKKLVLKEESL"
     gene            11355..12767
                     /locus_tag="K8M77_001494"
     CDS             11355..12767
                     /locus_tag="K8M77_001494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085991.1"
                     /translation="MNIKKYYSYKKIFLLLILYSIVLISSMLTTNINSIKLLPIIFSI
                     GITLLFLFKKDMNLIYKIIIFLFSIRMFFYPILCLIYGMSFEGYNFLIQNNIILSSFI
                     QCYEFMLIVLFLLIYKNNKEYNEEINIEDDISLNHKLLNCLKLLIILSIFIILIYPSI
                     LWKFKPIVFLSEVKELEWNRNANNVKNLIPGYTYNLALWLLLIIRILIVYLSIILLKR
                     RSKKSNESIFIVLSFLIILTLIIVSPDDKANSFFAVISFSAMLCKLYPDKKKNIMKIS
                     MLFILISVISILFIIPIIKQGEIDILFLNIRDRLNAYFGGTLNISAIFEMKRENLVNY
                     FIVDILRSIPLIMSFFSDIDTSNILFNSYLGKDIIYNSQILPNIGQGYFYFGFFLSPI
                     LSIMLLWKSLNNYNKAINLKNSFEYFINFYSAILCVLGIVLYNFTLTLNLFLQYIAPL
                     YLIYILFVKYEKNKKQKIIK"
BASE COUNT         5355 a         1088 c         2161 g         4255 t
ORIGIN      
        1 attaaaaaaa taaaataaaa aaaattaaat ttaaggataa acatgttgta aattgtaaaa
       61 cgaattaaat ttacctaaat attatgattt gataatatga aaatgtatta gagtaggata
      121 tataaacata ggaagtaaaa ggcgatttaa gaaggaattt aaaagaaaat aatattaata
      181 gtaggtgaaa ttatgagttt atgtaaagga acagttaaaa ttgggaaaaa aaatcttaaa
      241 aggggaacta ataagtattt ttttaagaga tatttaaaaa agaactatga gtttacagtt
      301 tcagaattta aggacagaaa taaatttgaa cttttagaac ctgttaatgt agaggggtta
      361 gattttttag ttgatgtgga atttaggggg attaggccaa aggaaataga tcttagaagc
      421 gttgataatt tagaaaaata tcaatttgaa agatacgaac agtgggtttc taacaattta
      481 ggaatttcct tagaaaatag caagtggaaa aataaaaagt ttaattgggg caatttaaat
      541 gttaagcttt caaagacagt aaaagaggat ttaggatgta taacaataaa agttgtatac
      601 aagtaatgat ttattttaaa tgagttaaat taataatatt tcttcttata aatatattaa
      661 taaaataaaa tactttgaaa tttataaaaa caaagttaac aaaaaattca tatttgtgaa
      721 tatttgttga aaaaagtcat atgtattatt tgtcaaaatg tgattttata agaaataagt
      781 agaattattt ggggatgaaa actatttatt ttgaaaaaat tagtattttt aagagggaat
      841 agcttatatt tttcaatcta actaatttac aaataaagta tatacagact taaacatttt
      901 tttaattaat tattttaatt atttttaaaa atataaggat atttattgaa aaataattcg
      961 ttaaaataga aaataaagaa aaaatagaaa ttacaggggg ctttatgaaa gaaaggtttt
     1021 agtatattat agttaggtga gactaatttg ttgaaaataa aaattaaata gagtaatagg
     1081 aggattcatg gaagaaaata ctataagctt acaagaaatt gcttacgctt taaagaagag
     1141 atggaaacta attgcactaa taaccatagc tgctacctta gtttcagcaa tattaagttt
     1201 ttttgttata aaaccacaat atgaggcaaa aactaagctt tttattggta agcaagaaac
     1261 aagtcaaaat gcagcaaact atgataataa tgatattatg atgtatcaaa aacttatgaa
     1321 gacttacgca gaacttgcaa aaacttcaga cttagttact aaggcagtta aaaatgctaa
     1381 attagattac aatcagaatg aaataaaagg ggttttaaat aatcttaatg ttactccaag
     1441 tgctgatact caaatattag atttaagctt taagggaaaa aatcctaagg aagttttaaa
     1501 ggtaacagag gctataacag atgaatttat ttcagaatcc aaggagctta ttccaaatgg
     1561 aaatgtacaa gttatacaaa aaccacagct tccagaaaat ccagtaagtc caaataagaa
     1621 gctgaatata cttattgctt ttgttttagg tcttatgata ggagtaggaa tagtactttt
     1681 acttgaatac ttagataata catttaaatc aagagaagaa ctggaaaaaa ctttagactt
     1741 acctataata ggagctattc cagactacga tagtatagaa aaataaggga ggaattagat
     1801 gttaatatta gagaaaaatc caaaatcagt ggcagctgaa agttatagaa ccttaagaac
     1861 aaacatacaa tactcttcct ttgataatcc agtaaaatct atagttatta ctagctctga
     1921 gccaggagaa ggtaaatcaa caacaagtgc taacttagct ctttcctttg cgcaagatgg
     1981 aaaaaaagta attcttatag attgtgattt aagaaagcct gttgtacata aagaatttca
     2041 aattagtaat tctagaggat tatcagaatt tctaattgga aatgttgaat tttctaaggt
     2101 tgtttacaaa catgaatcag gacttcatgt cttaccttca gggttagttc ctccaaatcc
     2161 agctgagatg ttagcttcaa gatctatgga gcatctttta actcaattag aagaaaagta
     2221 tgattacata atattagaca ctcctccagt taatgcggtg acagattcaa aaatactttc
     2281 aacaaaggtt gatggaacaa tacttgttgt aaagtatggt tatacaaaga aggatggagt
     2341 aatagaggct gtaaaaggac taagagcagt taaggctaat ataataggaa cagtatttaa
     2401 tggagaggaa aatcaaaggg gaaaatacta tcactattat aagaaagatt aatatttaaa
     2461 caaaacaact taacactcca aattaggagt gtaagttgtc aattttgatg taaaaaaact
     2521 gaaaaaaagt aataatatat taagggttaa agtgtttact ttatatagat aaaataatta
     2581 aagtagttag ttaagaaatt aatttggtta agggggagtt aagaaacgtg tccaaaataa
     2641 gtaaagtaaa aggaatttta ttagtactaa ttgatatact ccttatcaat atagcttata
     2701 tttttagtat gcttgttaga tttgccttta atggacctgt attaggagat attccaagag
     2761 aacaatggag tttatatttt aaatggatgc ctcttatagc agtaatatat gttgttctat
     2821 ttacattctt taaaatgtat aaaagtcttt ggagttttgc agggttagat gaagtaatga
     2881 aaggtgttgc ggcatgtgta ttaggatgtt taataaatgt tattattatg cttttcttaa
     2941 accctagaat tcctatgatg gtaactttat cagcaggaat attaacaatg gtacttactc
     3001 ttgggtttag actttcattt agaatgtata gaaggcttgt aatctatgga acattaaagg
     3061 gtattgaatc acaaaaaaat gttttaatag ttggagctgg ttcttgtgga agattagtta
     3121 ttaatgaaat gaaaagagaa acaggagctg ggtttaaacc tataggagtt atagatgatg
     3181 acagaagtaa gtttggaact tatataaatg gagtaaaagt cttaggaaat agaaatgata
     3241 taaaaaatat tgttgaaaat caaagagttg atttgatttt aatagctata gcatcaataa
     3301 cttctgatga aaagaaaaaa attatagaag cctgccatga aactaaggtt aaagttaaaa
     3361 taatgcctgg agtttatgaa atgataggtg gcaaggttaa cctaactaaa atgagagatg
     3421 ttgacttaag agatctttta ggaagagaag aggttaagct tagcaaggat gaaatagcta
     3481 attatataaa aaataaaaga gttttagtaa ctggtggagg gggatctatt ggttcagaac
     3541 tttgtagaca aatagctaat tttaatccaa aggaattaat gattttagat atttatgaaa
     3601 ataatgctta tgatttagaa atggaattaa atagaactca tccagaactt aaaaagcaag
     3661 taatcatagc ttcagttaga gataaagaaa agttagattc tatttttaga agtttcaaac
     3721 cacaagtagt attccatgca gcggctcata aacatgttcc tttaatggag acaagtccag
     3781 gagaggcaat aaaaaataat gctggaggaa ctttaaacac tgcagagtgt gcagataagt
     3841 atggagtaga aaaatttgta ttaatttcaa ctgataaggc tgttaaccca actaatgtaa
     3901 tgggagcaac taagagaatg tgtgaaatga taatacaagc tataaataaa aaaagtaaga
     3961 ctgattttgt tgcagtaaga tttggaaatg ttcttggaag caatggttca gtaattcctt
     4021 tatttaaaaa gcaaatagct gagggaggac cagttacctt aactaataag tatataacaa
     4081 gatattttat gcttattcca gaggcagctc aattagttct tcaagccgga gcatatgcta
     4141 aggggggaga aatctttgtt ttagatatgg gtaaaccagt taagatatat gatttagcaa
     4201 agaatctgat aaagctttca ggatatgaac cttttgaaga tatagatatt aaaatagttg
     4261 gcttaagacc tggagaaaag ctttatgaag agcttcttat ggatgaagaa ggattaacgg
     4321 aaacaaagca tgagaagatt tttataggaa aacctggaga ttttgatttt aactatataa
     4381 aatcaaatat agaagaactt atcaatataa gtagatttgg aacaggtgat gatataaggg
     4441 ataaaataga agaatttgtt ccaacataca atagattaga taaaaaggat aaaaatgaag
     4501 tagaatcttc taagttagaa gtagcaacta ctttatagat taattagata tggagtgata
     4561 aaagtgtcag aagaaaataa gagaatttat ttagcatctc ctcatatggg aggattagaa
     4621 gaagtatttg ttaaggaagc ttttgataca aattggatag cacctttagg agcaaatgta
     4681 gatggttttg aaaaagaatt atcagaatat gttggaagta agactggagc tgccttagct
     4741 tcagggacag cagctataca catggcatta aaggctgttg gagttgaaaa aggagataag
     4801 gttttttgct caagtttaac atttgcagca agctgtaatc caataattta tgaaggtgga
     4861 attcctatat tcatagattc tgaaccagaa agccataata tgtcaccagt tgctttagaa
     4921 aaagcattta aggtttatga agaaaaagga gaaataccta aggctgtaat agtagttaat
     4981 ctttatggac aaagtgctga tatggacaaa attattgaaa tatgtaaaaa gtataatact
     5041 ccaataatag aagatgcagc agaatcttta ggagcgactt ataaaggaaa gcatagtgga
     5101 acatttggag attatggaat atattcattt aatggaaaca agataataac tacttctggt
     5161 ggaggtatgt tagtttctaa taatgaagaa ggaatagcta aagtaagatt ttggagtact
     5221 caggctagag ataaagctag acactatgaa cacacagaac ttggttataa ctatagaatg
     5281 agtaatatag tagcaggaat aggaagagga caacttagag ttttagaaga tagaatagct
     5341 aagaaaaaag aaatttttga aacttataaa gaagctttta aagatataga agatatagaa
     5401 atgatgccag tatgtgagta tggagaacct aattattggt taacaacaat aacattaaat
     5461 gaaaattcaa aggttaaacc acttgatatt atattagctt tagaaaaaga gaatatagaa
     5521 tcaagaccaa tatggaaacc aatgcatata cagccttatt ataaggaata cgatttctat
     5581 tcacataatg atgaagaaga aatgagtgta tcagaagata tattcaatag aggagtatgt
     5641 ttaccctcag atacaaagat gacagataaa gaacaaaaaa gagtaataga aataataaaa
     5701 aacattttct gaaggggatt taaaagatga acttatatgg agataaaatt aagttaagag
     5761 cattagagcc agatgatatg gagtttttaa gagaaactgt taataatcca gaaattgaaa
     5821 aattagttgg tggatggtct tttcctatat ctaaaaagca acaaatggat tggtatgaaa
     5881 aaataataaa cgataaaaca tctttaaggt ttgcaataga gacagatgaa gatggtatta
     5941 ttggtatggc agacttaaga aatattgatt ggaaaaatag agtagctttt catggtataa
     6001 aaataggcaa taaagaatat agagggaaag gatatgggaa agatgttgta tttacaatta
     6061 tgaaatatgc atttgaagaa ttacagttaa ataggttaga agtaacaata cttgaaagta
     6121 atatagcttc tcaaaaattg tatgtagata aatgtgggtt ggttgttgaa ggcgtaagaa
     6181 ggaattgcgt atttaaaaat aataagtatc ataatcagct tgtacttgga attttaagag
     6241 aagaatattt taatcttaaa aacaaataga tggtgataaa atgtataaaa attatttgaa
     6301 aagagtatta gatgtaatat ttgctagcgt tggtattgta gtttttttcc ctatatatat
     6361 attagttgca gtaatggtga gaataaattt aggatctcca ataatattta aacagaaaag
     6421 gcctggtaaa gatgaaaaaa tttttaccat gtataaattt agaagtatga gtgatcaaaa
     6481 agataaaaat ggaaatttgc tttctgataa agataggctt actaaatttg gaagagttct
     6541 tagatcaact tctctagatg aacttccaga attatttaat atattaaaag gggatatgag
     6601 tatagttgga ccaagacctc tagctatagt ctatcttgaa tattataatg aagaggagaa
     6661 aaaaagacat agcgttagac cgggacttac aggcttagct cagataaatg gaagaaattt
     6721 atcaaattgg gatgaaagat ttaaaaatga tatagagtat gttgagaata tatcttttac
     6781 taatgatttt aaaataatac ttaaaactat taaaaaagta ttattaaaag aagatgttgt
     6841 tgcaagaggt gaaggaaaaa ctttagattt tcatatttat agacaaaagc aattggaaga
     6901 aaataataaa ttaaagaggt attaaatgaa aagagaaata ggaagttttt tttgggaata
     6961 taaaatcgat aatacagaga ataatttaag tttttttgaa aacttaggaa aagataaatt
     7021 gtttttatct tcagggagag atgctttaaa gaaacttata aaagaaatag gtaaaaacaa
     7081 gactatactt ttaccaggat ttacatgtag ctctgtaatt ataccttttg taaataatgg
     7141 atataaagtt catttttatg atttagaaaa taatctagaa gttgatgttg aattgttctt
     7201 aagaaaaata aaagaagtat ctcctgatat aatattaatt cattcttact ttggatttga
     7261 tactataaaa aatataagac cattgttaaa agatataagg aaaaataata ttatagtagt
     7321 agaagatata acgcaaagaa tttattcaaa attcgatttt gttcaatcag attatatagt
     7381 atcaagtcta agaaaatggt ttgctatacc agatggtgga gttttgataa atagattgaa
     7441 tgatttaaaa ttaaaagaag ataaaaaaat aaataataag ttatatgatt tatctttaaa
     7501 aatgtatgat attaaaaaac aatatattga aaatggagat ttgatattaa aagaaaaata
     7561 ctatgaaatt tttaagaaaa ctaaagttga atttgaactt tggaatgaaa tttacagtat
     7621 gtctaatagg tcaattgaaa tatataatag tgttaattta gattttataa attataaaag
     7681 atatgaaaat tataaatatt taatagaaaa tttaatcaat ttagaaggca tagaaataat
     7741 ttttaagagt attgataaaa atattgtccc attatatttt cctattttta ttaaaaatgg
     7801 gaagagggat gaaatccaaa ggaagatggc taaaagaaat atatattgtc caattgtgtg
     7861 gcccatgtcg aaatttgttt atagaaaaat aaattctaaa acaataaata tatataatga
     7921 aatattatgt atcccatgtg atcaaaggta tgatatagat gaaattaagt ttgttgtaga
     7981 taatttaaag atagttatta ataatagtat tttataattc tattattaga taataaagga
     8041 gaagatatgg ttaaactaga aaaggatatt tattttgaag aaaactattc aaaattatat
     8101 gaagatatag aaaatggaaa aacaaaagtt tttagactaa attctgaata tggggagata
     8161 gaaaatattt tcataaaaag aaaaatagat ttttataaaa aagaagaaat ttatgatata
     8221 acgactccat atgggtatgg aggccctaga atagtttggg tagataatga caataaagat
     8281 aaattaatta aagaatatga aaaaaaattt aaaaaatttt gtaaaaaaga aaatataata
     8341 gcagaattta ttaggtttaa tcctataatt ggtaatcatg aatattttaa agatatttat
     8401 gatgtgaaaa acataagaaa aactttagct acaaatttaa aagattataa agatcctatt
     8461 aaggaggaat tttctaaaag tgctaggaaa acaattagac aagttttaaa aaaaggagtt
     8521 acatataagg ttattcagtg tccaaatgaa ttggaagagt ttaaaaaaat atactactcg
     8581 actatggata gaaaaaatgc agatgattat tattattttg atgatgaata tttcaataaa
     8641 tgtttagaat tttttaagga aaatataatt agtattcaag taatatttga agaaaagatt
     8701 atagctgcaa gtttatattt tgtatacaat aatattttgg aagcacatct ttctggaaca
     8761 ttaagtgaat atctatacat gtcaccagca tatataatca agtatgcaac tgctttatgg
     8821 gctaaggaaa ataatataga tataatacat tatggtggtg gaacttcaaa taatatagat
     8881 gatccattat atacttttaa aaaaaagttt tctcaaaaaa cagagttaga tttttatatt
     8941 ggaaaaaaaa tatggaactt aaatttatat aaagaaatat gtgatttaaa gaatgttaat
     9001 tatgaagatg aatattttcc tgcatataga aaatagaact tttataagtt agtacattta
     9061 atttggagat agttatgaaa aaagatatat taatgataac tcattttact caagtaccat
     9121 gggaagctgg gaatggacgt tttctttatt tactaaatat gtttgactat gagaaagtga
     9181 aagttgaatt agttacaact gatttttcac atagaactaa agaaaaaaga aaaaaaaaca
     9241 atgaaaatct aaggtataaa cttacatatg ttgaggaatt aggatataaa aaaaatgtat
     9301 ctataaagag gttttttagt cattatatat ttgcaaaaaa tttgaggaaa tatttagtta
     9361 gtagaaaaaa gccagatgta atttattgtt ctattccttc tacagatgca gcttattatg
     9421 taactaaata tgcaaaaaaa aataatataa aattaattat agacatacaa gatttatggc
     9481 cagaagcttt taagatggtt tttaatatac ctattattag cgatattatt ttttatccaa
     9541 tgaacaggaa agcaaatttt gtttataaaa atgctgataa aataatagga gtttcagaaa
     9601 cttatgtaaa tagagcttta agtgttaata gaaaagttga aaaaggatta agtgtttttt
     9661 taggtacgga tttgaattat tttgattctt taataaaaaa tgatgaaaaa tataatgatt
     9721 ttactatagt ttatgtaggt acattaggac atagttataa cataaaatta ataattgatg
     9781 ctttagagca agttaaatta aagtataata aaaatataaa tttcttaatt atgggagatg
     9841 ggccattgaa gaatgagttc caatcttatt ctaaaaataa aaatttacaa attgaattta
     9901 caggtaggtt aaaatatgaa gatatggtat atagattgaa aaaatgtcat ttagctgtaa
     9961 atcctattgt agatggttcg gctgggagta taataaataa ggttggagat tatgctgctg
    10021 caggattacc tgtcataaat acacagactt gtgtagagta taaagaatta atagagaaat
    10081 ataatctggg attttcatgt ttgaataatg atatagatgg aattgctaat aaaatttttg
    10141 agttatataa agatgaaaat cttagaaaaa aatttggagc aaataataga atggttgcag
    10201 aaattttatt tgatagaggt aaaacttata aaaaaattat taatataatt atgggagaag
    10261 aggatgaaaa tattacatat tgcatcaata actgataata aagcaagtgg aatgagtgta
    10321 gctataccaa agcatgttaa atttcaaggc tactatgcag aaactgcatt gttaaatttg
    10381 aatttaaatc aattagaaga acattcaatt agaattttta atttaaataa tttaaataga
    10441 caaggaatta gtaatttgcc taataatttt tcaaggccag atttagtagt tttacatggt
    10501 atttattata ttaaattttt aacgatctac cgatatttaa gaaaaaataa aataccatac
    10561 gttttagttc ctcatggatg tttaagtaaa tatgcaataa ataagaaaaa aattaaaaaa
    10621 aaattagctc taaagctatt atttaataag attataaata actgtgtatc aatacaatat
    10681 ttatccgaaa tagaaaaaga aatttcattt ggagattcta aaaaaaattt tatatcacca
    10741 aatggaattg aaattaattt taacaataaa atatataatt taggaagaga attaaattta
    10801 atttatattg gaagaatttc gatattagaa aaaggtctag acttaatgat tgaagcaata
    10861 aatttaatac aaaatattat gagagaaaac aatgcaaaat taaatatata tggttcagat
    10921 gtagatgatg gaaaaaatat attacttagt atgtgtaaaa aatataaaat agaggatata
    10981 gttaaaataa atgatgctat ttttgataag aataaaatag aagtaattaa caattcagat
    11041 atttttattc agacatctag aacagagggt caaccaatgg gtattttaga ggctatgtca
    11101 tatggattac ctattatagc tacaaaagga acctcttttg cagatgatat taaagaaaat
    11161 ttatgtggat ggagttgtga aaataatata gaagatatag ctatggctat aaagaaagcc
    11221 tgtgatgaaa aaaaatatat agaatgttat ggtaaaaatt ctagagagtt tgtaaataga
    11281 aaatatagct gggatattat ttctagagaa gctatagagg aatataaaaa attagtatta
    11341 aaagaggaaa gcttatgaat ataaaaaagt attacagcta taaaaaaatt tttttattgc
    11401 ttatattata ttcaatagtt ttaataagta gtatgttaac tactaatata aatagtataa
    11461 aattattacc tataattttt tccataggaa taactttgtt gtttttattt aaaaaagata
    11521 tgaatttaat ttataaaata ataatatttt tgttttctat aagaatgttt ttttatccaa
    11581 tattatgttt aatttatgga atgagttttg aggggtataa tttcttaatt caaaataata
    11641 ttatattaag ttcttttatt caatgttatg aatttatgtt aatagttttg tttcttttaa
    11701 tatataaaaa taacaaagag tataacgaag agataaatat tgaagatgat attagtttaa
    11761 atcataagtt attaaattgt ttaaaattat taataatatt aagtattttt attatattaa
    11821 tatatccaag tatattatgg aaatttaaac caatagtgtt tttaagtgaa gttaaagaac
    11881 ttgaatggaa tagaaatgct aataatgtta aaaatttaat tcctggatat acgtataatt
    11941 tagctttatg gttattatta atcattagaa tacttatagt ttatttaagt ataattttat
    12001 taaaaagaag atcaaaaaaa tctaatgaaa gtatttttat agtattgtca tttttaataa
    12061 ttttaacatt gataatagta tcaccagatg ataaggcaaa tagttttttt gctgtaataa
    12121 gtttttctgc tatgctttgt aaactatatc cagacaaaaa gaaaaatata atgaaaattt
    12181 ctatgctatt tatattaata agtgtaatat ctatattgtt tataattcca ataattaagc
    12241 aaggcgaaat tgatatatta tttttaaata ttagagatag actaaacgca tattttggtg
    12301 gaacattaaa tatatcagct atatttgaaa tgaaaagaga gaatttagtt aactatttca
    12361 tagttgatat tttaagatct attccattaa ttatgagttt tttttcagat atagatacta
    12421 gtaatatatt atttaatagt tatttaggta aagatataat atataattct cagatattac
    12481 ctaacattgg acaaggatat ttttattttg gtttttttct atcaccaatc ttatctataa
    12541 tgttattatg gaaatcttta aataattata ataaagcaat aaatttaaaa aatagttttg
    12601 aatattttat taatttttat tcagctattt tatgtgtttt aggtattgtt ttgtacaatt
    12661 ttacacttac tttaaatttg tttttacagt atattgcacc attatatcta atttacatat
    12721 tatttgtaaa atatgaaaaa aataaaaagc aaaaaataat taaataggtg aggataaatg
    12781 aaagttttaa tttacatgaa tattaataat ttgaagccaa ctggtggacc agcaggttat
    12841 ttatataatc ttaaaaatg
//
LOCUS       DAEVGI010000064        12830 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEVGI010000064 DAEVGI010000000
VERSION     DAEVGI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12830
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.064"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            63..1556
                     /locus_tag="K8M77_001495"
     CDS             63..1556
                     /locus_tag="K8M77_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBI7085992.1"
                     /translation="MNRVLDILKDRSLTYHQQIKDLVAYAESTIEVLNIDDETKKLID
                     EGCICTLFEGNAPFRPRYIIPDYSLLMEKGCEFLNLSVPEDIWEATNNLLIFYKHVPS
                     ITTFPVYLGNIDEFLEPFIEDEREAKRAIKLFLKHIDRTLTDSFVHANIGPRETVAGR
                     LILESMDELQCAVPNLSMKYDLENTPESFINLCAKVALSTAKPSFANHKMDVKSFGTD
                     RYAIASCYNGFHIGGGGYTLVRIKLNEVAKKATSVEDFLENVLPYVSTKMIQYIDERI
                     RFLVEETQFFTSNFLVKEGFIQKKLFTGMFGVVGLAEAVNTLLGAEKQSDRFGYSEKA
                     NKLGLRIIEKLNKIVSEHEAKHVNCFGGHYVLHAQVGIDLDQNVSPGCRIPVGEEPEL
                     FNHIMQSAPFHKYFYNGIGDIFVFEDTYNDHPEALVDIIKGAFNSGIRYMSVYGSGAD
                     VVRVTGYLAKRSEVEKLERGEAVLNNTDLFAMGAKNNCHAFERKIRK"
     gene            1565..2386
                     /locus_tag="K8M77_001496"
     CDS             1565..2386
                     /locus_tag="K8M77_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBI7085993.1"
                     /translation="MALINKIIPFSCVDGPGNRMVIFFQGCNFKCFYCHNPETINKCI
                     SCGKCVENCEVGALSISDGKVIWDKEECISCDKCIKLCEHMSSPKLKEYSVEELVKKI
                     EKDSFFIRGITVSGGECTLNSEFLIKLFREVKKLGLTCFADTNGNTKLDDELINLTDK
                     FMLDVKSIDEKENIWLTKSSNKLVLENFKKLLELDKMYEVRTVIAKGLNNEKTVDEVS
                     KIIGDKCRYKLIKYRPFGVREEGIKVHGTISPEDSYMNELKEKSIANGCIDTIIT"
     gene            2678..3193
                     /locus_tag="K8M77_001497"
     CDS             2678..3193
                     /locus_tag="K8M77_001497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085994.1"
                     /translation="MKKFFIMVLSIIIIVGGVLFYRFKTRKFLNFNLESISIEQATES
                     DFSNSLSKDNKNEEPLDYFDIVFSIRAKNTSKNLCTDNIVPHIILPKELKSNGYIDIG
                     LNPLSSTDEIILPGHEKGFPYKLFVKKGKYSKEELLKLLKECKFKVNIVSFKKTKMQF
                     LTYGGSVEYIE"
     gene            complement(3297..4100)
                     /locus_tag="K8M77_001498"
     CDS             complement(3297..4100)
                     /locus_tag="K8M77_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI7085995.1"
                     /translation="MNIAYDFIIALILAFVLVMMKQYVFALIVVVIYALYKYNKKKPY
                     ILAYKGNVSFRNGEREKALELYKKACTYKNASDSIRLQYSYLTLYLGNLEEATKLLSA
                     IDYDKLPERLKASYTMTDSLITWKNGDLKKAIENLKNLHTQYEHTTVYETLGYFLILD
                     KRYEEALKYNLLAYEYDQDNKVIMDNLAQSYYFTGNIEKAKEVYTKLLDEDPGIPEPY
                     YYYGLILRDEGDTKGALESFDKALTKRESYLSALNKDKIRAAIQELNIK"
     gene            complement(4423..5733)
                     /locus_tag="K8M77_001499"
     CDS             complement(4423..5733)
                     /locus_tag="K8M77_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HBI7085996.1"
                     /translation="MNTTLANLTPTKEQRKYQEDELIAFLDLQMNTFTANSENNDEAP
                     SLLKQETSFNTDIWIENLFKFGFKKVILTAKLNNGVCLWQSNCSKVNINTFSLENSYD
                     DLVKKVSKSCKKFGLKFGIHLTLKDYLTLNLTPEEYDNYYTSQLKELITNYSQISEVI
                     MDMTSLDEYYDSLDFKRYFKVIKEINPKCIISSPIGPDVRWTYYSDIESSKNYFYSSI
                     NLNLLKEDFNNEEIRKGNIYGDTWIVGESIYSLSDCLNHNKDSLSNLKHVYNNSLGRN
                     TNLVLVLSPNTDGLLNHNELSLLSDFSKYIKETFSNNLIKGSSILATNSSSSDSYNLI
                     DDYKKSYWIANENAVNPYIEIDFKTITEFNILEIREWIAEGQNVEEFKVYAYNNSWFE
                     LYNGTSIGYRHIAKLNNIKTDKIKISFTKYKNPPMINHIGAYLG"
     gene            complement(6024..6350)
                     /locus_tag="K8M77_001500"
     CDS             complement(6024..6350)
                     /locus_tag="K8M77_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6240..6242),aa:Sec)
                     /transl_table=11
                     /product="HesB-like protein"
                     /protein_id="HBI7085997.1"
                     /translation="MSVVKMSNEAYTEFKSFLQENGVEKFDIRINLAGVGUGGPVFNI
                     VLDEQSDNDEVVKIEDITFFVDKELVKDFEGFTLLSSDENGGRGLSLKPVKESEGGCS
                     SCSSCH"
     gene            complement(6452..6796)
                     /locus_tag="K8M77_001501"
     CDS             complement(6452..6796)
                     /locus_tag="K8M77_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB-like protein"
                     /protein_id="HBI7085998.1"
                     /translation="MKKVAMSDDAYIEFKEFLKSNDIDSDVIRINFGGNGPMGPMFYV
                     TAGEKNEGDEVEVIKDLTFVIDSSLIDVFLGFSILSDEENFGEGLTLDPLMSDYEGGG
                     CSGNCGSCGSCH"
     gene            7251..7982
                     /locus_tag="K8M77_001502"
     CDS             7251..7982
                     /locus_tag="K8M77_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7085999.1"
                     /translation="MVPYNIDYCEEKIKEYKNKIKENKKGDFIIRYSEGKEFDGYLYE
                     HKQSLKESPIELYEGEKLWMRISPHEIQGAFEGIKRAKGKVGVLGLGLGYFVQEIAKS
                     DQVREIVVYEMSEEIIDLYLENFGENPKIRIVKGDGFKAKRENFDFFYVDIYEYKLTT
                     KVVEDYVKLTKLHDIVEYSFFGVESFILSCPTSEIIWVYILEEWMDMSKDLFTRFNHS
                     EYIEYFSPIEENKVLEVLKEFGEVL"
     gene            8485..9492
                     /locus_tag="K8M77_001503"
     CDS             8485..9492
                     /locus_tag="K8M77_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086000.1"
                     /translation="MAHKKVAKIIATAIVVNNLSSSVVLGKEVNLIDKNKDNSVINQN
                     ISHSKRHNNIFRIEKTVGNTEEFLKIINDGNLEEIKLSRDIDLSNLISSGVDIKSSNV
                     VIDGQGYSIYVPKLAENLNRDFFTLQGDGIVLKNLNIVCKDDEDLNNNNLITISGDDI
                     TLENVFVESNFNRAVNILEGNNIHINDCKIVNNQEKGNGLKVENGIVTLNNLDLQNKS
                     GVGLDILGKDSKVYLKGDIKINSPIEIRGQFRDGGILNCDNNQLIHEKRVFGYTYYNV
                     AKETELVRNKDEFLEAIDNNIVKNIKLMDNIDLRGYESEYYKIFEKEIKVDKNNYHIT
                     M"
     gene            complement(9638..12088)
                     /locus_tag="K8M77_001504"
     CDS             complement(9638..12088)
                     /locus_tag="K8M77_001504"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBI7086001.1"
                     /translation="MGNYSTAIDKKWQDKWAESGLYKFDPNKEVEKLYVLEMFSYPSG
                     SQLHAGHWFNYGPVDSWARFKRMQGYNVFQPMGFDAFGLPAENFAIKTGIHPQDSTIK
                     NIAKMEEQLKAMGAMFNWENEVVTCSPEYYKWTQWLFLKLYEKGLAYRKKAPVNWCPS
                     CQTVLANEQVVDGACERCSTEVTKKDLTQWFFKITDYADELLDKLDGLDWPEKTVSMQ
                     KHWIGRSTGSQVNFKVKDSDLNFDVFTTRVDTLCGVSYVVLAPENPLVDEIVSAEQKE
                     AVENYKEEAKKQSDIERQSISREKTGVFTGAYAIHPLTGKEVPIWVGDYVLATYGTGA
                     VMAVPAHDERDFAFAEKFNLPINRVIEAKDGSETNLPFCEHGILVNSGEFDGLTTDEA
                     KEKIVEKLASMGLGEKKVNFRLRDWLVSRQRYWGAPIPVVYCEECGIVPVPESQLPVE
                     LPYDVEFAPDGKSPLAKSEAFVNTTCPHCGKPAKRETDTLDTFVCSSWYYLRYPDNKN
                     TEAPFNPELINKMLPVDKYVGGPEHACMHLLYARFITKALRDMGYLNFDEPFTSLTHQ
                     GLILGPDGLKMSKSKGNTISPDDYIKEYGADVFRMYLMFGFAYTEGGAWSDDGIKSVN
                     RFVERIERIIDTAREAISKGENNKTTMDKAEKELNYWRHNTIKSVTDDTDKLQFNTAI
                     ARMMEFINALSKYTQEKEMNLDFLKDVVSDYLRLLAPFAPHFSEEQWNLLGNSYSIFN
                     EAWPKFDPKALVKDEVEIAIQVNGKIKNKIMVSSDLDEEGIKAAALADEKIIASTEGK
                     TVVKVIVIKGRLVNIVVK"
     misc_binding    complement(12176..12383)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12527..12670
                     /locus_tag="K8M77_001505"
     CDS             12527..12670
                     /locus_tag="K8M77_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086002.1"
                     /translation="MGKKLKSATNNQWVSTTVDPNMVPAKDRQPKTAGDNQWTSTSKN
                     PMK"
BASE COUNT         4770 a         1573 c         1896 g         4591 t
ORIGIN      
        1 ttgaaaagag caatgttcat ttaacttaac agatcgtata tgttttaaag gagaggaaaa
       61 gtatgaatag agtattagat atactaaagg ataggtcatt aacatatcat cagcaaatta
      121 aagatttagt tgcttatgca gaaagcacaa ttgaagtgtt aaatattgat gatgaaacaa
      181 aaaaattaat agatgaaggg tgtatatgta ctctttttga ggggaatgca ccatttaggc
      241 ctagatacat aattccagat tattctttac ttatggaaaa gggatgtgaa tttttaaatc
      301 tttcagttcc agaggatata tgggaagcaa ctaataacct attaattttc tataagcatg
      361 tgccttcaat aactacattt ccagtttacc ttggaaatat agatgagttt ttagagccat
      421 ttatagaaga tgaaagggaa gctaaaagag caattaaatt atttttaaag cacatagata
      481 gaacattaac tgattctttt gtacatgcta atatagggcc aagagagact gtagcaggaa
      541 gattaatact agagtctatg gatgaacttc aatgtgcagt tccaaattta agtatgaagt
      601 atgatttaga aaatactcca gagagtttta taaatttatg tgcaaaggtt gctttaagta
      661 cagctaaacc tagttttgca aatcataaaa tggatgtgaa aagctttgga acagatagat
      721 atgctatagc aagttgttat aatggatttc atattggtgg gggtggatat acccttgtaa
      781 gaataaaatt aaatgaggta gctaagaagg ctacttcagt agaggacttt ttagagaatg
      841 tattacctta tgtgagcact aaaatgattc agtatattga tgaaagaata agatttttag
      901 ttgaggaaac tcagttcttt acttctaatt ttttagtaaa agaaggattt attcagaaaa
      961 aattatttac aggaatgttt ggagtagtag gtctagcaga ggctgtaaat actttattag
     1021 gagcagagaa acaaagtgat agatttgggt attcagaaaa agctaataaa cttggattaa
     1081 gaataataga gaaattaaat aaaatagtat cagaacatga agctaaacat gtaaattgct
     1141 ttggaggaca ctatgttctt catgcacaag taggaattga tttggatcaa aatgtatcac
     1201 caggatgtag aataccagtt ggagaagaac cagaattatt taatcatata atgcaatcag
     1261 caccattcca taaatatttt tataatggaa taggagatat atttgtattt gaagatacat
     1321 ataatgatca tccagaagcc ttagttgata ttataaaggg tgcctttaat tcaggaataa
     1381 gatacatgtc tgtttatgga agtggagcag atgtagtaag ggtaactgga tatttagcta
     1441 agcgttcaga ggtggagaaa cttgaaagag gagaggctgt tcttaataat acagacttat
     1501 ttgccatggg agcaaagaat aattgtcatg catttgaaag aaaaattaga aagtaagtga
     1561 tattatggct ttaataaata agataattcc attttcttgt gttgatggac caggaaatag
     1621 gatggttata ttttttcaag gatgtaattt taagtgtttc tattgccaca atcctgaaac
     1681 tataaataaa tgtatatctt gtggaaaatg cgttgaaaat tgtgaagtag gagctttatc
     1741 aattagtgat ggaaaagtta tttgggataa ggaagagtgt atttcttgtg ataaatgtat
     1801 aaagctatgt gaacatatgt cttctcctaa acttaaggaa tattcagtag aagagcttgt
     1861 aaaaaagata gaaaaagaca gtttttttat aagaggaata actgttagtg gtggagaatg
     1921 cactttaaac tctgaatttt taataaaact ttttagagag gttaaaaagc ttggattgac
     1981 ttgttttgct gatactaatg gcaatactaa attagatgat gaacttataa atcttacaga
     2041 taagtttatg cttgatgtta agtcaattga tgaaaaagaa aatatttggc taactaaatc
     2101 atcaaataaa ttagttttag aaaattttaa aaaattatta gagttagaca agatgtatga
     2161 agttagaact gttatagcta aaggactaaa taacgaaaaa acagtggatg aagtatctaa
     2221 aatcattggt gataaatgta gatataaact tataaaatat agaccctttg gagttagaga
     2281 agaggggata aaggttcatg gaactataag cccagaagat agctatatga atgaattaaa
     2341 agaaaaatct atagctaatg gatgtattga tacaataata acttaatatt ttttagctaa
     2401 gtaaatttta tttattattt taatcataaa ttattttaat tttttatttg tgtaaatgtt
     2461 aatagataat tttatttaaa tattaaaaat cttcttttgt ttaagttgga aatagcttct
     2521 aagctataaa aaacaaaaga aggttttttt atgtgttaaa atagttatag cgttcaaaaa
     2581 tgaacttaat agtttaactt ttaaataagt aaagagaata tagtttaaaa tttatatgta
     2641 catataaaat catattttgt atttaaggag aaaccctatg aagaaatttt ttataatggt
     2701 actttctata ataattatag tggggggagt attattttat agatttaaaa caagaaagtt
     2761 tttaaacttt aatttagaga gtatttctat agagcaagct actgaaagtg attttagtaa
     2821 tagcctatca aaagataata aaaatgaaga gcctttagat tattttgata tagtttttag
     2881 tattagagct aaaaatacat ctaaaaattt atgcacagac aatatagtac cacatattat
     2941 attgcctaaa gaattaaagt caaatggtta tattgatatt ggattaaatc ctttaagtag
     3001 tacagatgag ataatattac caggtcatga aaagggattt ccttataaac tttttgtgaa
     3061 aaagggaaaa tattcaaaag aagagctttt aaagctttta aaagagtgta agtttaaagt
     3121 taatatagtt agctttaaaa aaactaaaat gcagttttta acctatggag gaagtgtgga
     3181 gtatattgaa taatttaatg agttttaatt aataacaagt ttcaagatag aaagtaaatt
     3241 aacaaataag actatatgaa gttttgattt catatagtct tatttgttta taaatattat
     3301 tttatgttta attcttgtat agcagctcta attttatcct tattaagggc tgaaagataa
     3361 gactctcttt tagttaaggc tttatcgaaa gattctaaag ctcctttagt gtctccttca
     3421 tctcttagga ttaagccata ataataatat ggttctggta ttccagggtc ctcatctaaa
     3481 agtttagtat atacttcctt agccttttct atatttccag tgaaataata agattgagct
     3541 aagttatcca ttataacctt attatcttga tcatattcat aagctaaaag gttatatttt
     3601 aaggcttctt catatctttt atctaatatt aagaaataac ctaaagtctc ataaacagtt
     3661 gtgtgttcat attgtgtatg aagatttttt aagttttcaa tagccttttt taaatcacca
     3721 tttttccaag ttataagaga atctgtcatt gtataagaag cctttaatct ttcaggtagt
     3781 ttatcataat caatagcact tagaagctta gtggcttctt ctaaattccc taagtaaaga
     3841 gtaagatatg aatattgaag tcttatgcta tcactagcat ttttatatgt acaagctttt
     3901 ttataaagtt ctagagcttt ttctctttca ccatttctaa aggaaacatt acctttgtaa
     3961 gctaaaatat aaggcttttt cttattatat ttgtataaag catagattac tacaacaatt
     4021 aatgcaaaaa catattgctt catcattact aatacaaatg ctaatattaa agcaattata
     4081 aagtcataag ctatgttcat tttttacgcc tccagtaaat acaaaatatt gcaattatat
     4141 tataagaata attttaatgg aattttattc ttaatacaac tttacttaat tatatttaca
     4201 aaaaaattat tttaatttaa tagtgaaata aaagagggtt gtaccaaaac aaaaatcaat
     4261 aaaacttatg aaaaatacaa agaaaacata tttataagtt ttaaatataa gttttgatat
     4321 aacccttttt ctttaaaaag caaaaaaact ctactaagga tagttttaag ctttcacttt
     4381 ttatatttat ttttgtattt tataaaactt tatttattaa aattatccta aatatgcacc
     4441 aatatgatta atcataggtg ggtttttata tttagtaaat gaaattttaa ttttatcagt
     4501 tttgatatta ttaagttttg ctatatgtct atatccaata gaagtaccat tataaagttc
     4561 aaaccaacta ttgttatatg cataaacttt aaattcttct acgttttgac cttcagcaat
     4621 ccactctcta atttctaaaa tattaaattc agtgatagtt ttaaaatcta tttctatata
     4681 agggttaaca gcattttcat tagctatcca ataagatttt ttgtagtcat caattaaatt
     4741 atagctatcg ctagaagatg aatttgtagc taatatagaa gaacccttaa taaggttatt
     4801 agaaaaagtt tcttttatat atttagaaaa atcagaaagt aaacttaatt cattatgatt
     4861 taataatcca tctgtattag gagataaaac taataccaag tttgtatttc ttcctaatga
     4921 attattatac acatgtttta aatttgataa tgaatcttta ttatggttta agcagtcaga
     4981 tagggagtaa atactttctc caacaatcca agtgtctcca taaatatttc cttttctaat
     5041 ttcttcattg ttaaaatctt cttttaaaag atttaaattt atagaagagt agaaatagtt
     5101 tttagatgac tctatatcag aataatatgt ccaacgtaca tctggtccaa tagggctaga
     5161 tatgatacat ttaggattta tttctttaat tactttaaag tatcttttaa aatcaaggga
     5221 gtcataatat tcatctaaag aagtcatatc cataataact tcacttattt gtgaataatt
     5281 agtaataagt tcttttaatt gagacgtgta gtaattatcg tattcttcag gagttaagtt
     5341 taaagtgagg taatctttta aagtaagatg aataccaaat ttaagtccaa actttttaca
     5401 agatttacta acttttttta ctaaatcatc ataactattc tctaaagaaa aagtatttat
     5461 attaaccttt gaacaattgc tttgccataa acaaacacca ttattaagct tagcagttaa
     5521 aataactttc ttaaatccaa atttaaataa attttctatc catatatctg tgttaaatga
     5581 agtctcttgt tttaataaag aaggagcttc atcattattt tcagaattag ctgtaaatgt
     5641 attcatttgg aggtctaaaa aagctattag ttcatcttct tggtattttc tttgttcttt
     5701 agttggagtt aggtttgcta gagttgtatt cattatctct cctccttaaa aataaagcgt
     5761 ttattaaatt ataatactaa aattaaacat aaataaccat attagtaaaa atattgaaat
     5821 ttaatttagt ttaaaaattt ttaaagaata aaatgaagaa gagtaaatta aattttctta
     5881 gtattttaag tagtataaat taattttaaa agacttaaat aaaatttaca tttatataag
     5941 ttaaatttat tttttattag aaggttaatt taagagaaga taaaaaaata gagatggaat
     6001 aaaatccatc tctatatgta aaattaatga cagcttgagc aagaagagca tccgccttcg
     6061 ctttctttaa ctggttttaa tgataatcct ctaccaccat tttcatctga agaaagcaga
     6121 gtgaatcctt caaaatcttt aacaagttct ttatcaacga agaatgttat atcttcaatt
     6181 ttaacaactt catcattatc tgattgttca tccagaacaa tattaaatac tgggccgcct
     6241 cagccaactc ctgctagatt tattcttatg tcaaattttt ctacgccgtt ttcttgtaga
     6301 aaagatttga attcagtata agcttcattg ctcattttaa caactgacat atctatttcc
     6361 tcctaaattt taatttaaag caaagttgcc ttatatagtt tattattaat atattttaaa
     6421 ataatatcac actaaaatag aaatattata attagtgaca gctaccacag ctaccacagt
     6481 ttccactaca accaccacct tcatagtcgc tcattaatgg atcaagagtt aatccttctc
     6541 caaagttttc ttcatcagaa agtattgaga aacctaaaaa tacatcgatt aaagaagaat
     6601 caataacaaa agttaaatct tttattactt caacctcatc accttcattt ttttctcctg
     6661 cagttacata aaacataggg cccataggtc cgttcccacc aaagtttatt ctaataacat
     6721 cactatctat atcatttgat tttaggaact ctttaaattc aatataagca tcatcgctca
     6781 ttgctacctt tttcatttga tcacctactt aattttaatt aattaataat taatattaat
     6841 tgattaaatt atataatttt aaacaaaata aatcaagact attttagtag gaatttaaaa
     6901 tggaaatttc tgttttgtat ataaatagta aaaagtagaa aataattatt atgtttttgt
     6961 aataatatga aaatttttat gaaaaatata ttgagaagtt ccttacatca aattaaaata
     7021 aaagagttaa ataaaatacg aaaacatact aaaaatataa ttattatgaa ctttaaggtt
     7081 caacgtttta ataaaagttt atatctcaat taaaggttga tttcatatat atttagatct
     7141 aacttaattt agtaagtact tttattaatg tagtttttaa taatatatct aaaagaaata
     7201 tagactaata aaaatgaatt gttttctgag aagattagaa aggaaaaagt atggtaccat
     7261 ataacataga ttattgtgaa gaaaaaataa aagaatataa aaataaaata aaagagaata
     7321 aaaaagggga tttcattatt agatatagtg agggaaaaga atttgatggc tatttatatg
     7381 aacataagca gtcattaaaa gaaagtccta tagaactata tgaaggagaa aagctttgga
     7441 tgagaatttc accgcatgag attcaaggag cttttgaagg gataaaaaga gcaaaaggta
     7501 aggttggggt tctaggatta ggattaggat actttgttca ggaaatagca aaaagtgatc
     7561 aagtaagaga aatagttgtc tatgaaatga gtgaagaaat aattgactta tacttagaaa
     7621 actttggaga aaatcctaag ataagaatag taaaaggaga tgggtttaaa gctaagagag
     7681 aaaactttga ctttttctat gtggatatat atgaatataa attaactact aaagttgttg
     7741 aagactatgt taaattaaca aaacttcatg atattgtaga atatagcttt tttggagtag
     7801 agtcatttat attaagttgt ccaactagtg aaataatatg ggtttatata ttagaagagt
     7861 ggatggatat gtcaaaggat ttatttacaa gatttaatca cagtgagtat attgaatatt
     7921 tttcacctat agaggaaaat aaggtattag aggtacttaa agaatttgga gaagttcttt
     7981 aacctttatg gttaatttat aaagagaaat attttaaatt tattttactt attaattgat
     8041 ttactaatta gttagaaatt ttaaattgat aattttgaac ttaggttata atatgattta
     8101 ttatgaaatt attatcttat attaattgta ataaggattt taatttatga aacatgttat
     8161 aacctttttg tatttaaatt ttaactttct caattataat agaatatata atttagagta
     8221 atatagaaat tttagatcat agtatttatt taattaagga aagaatattg ctttgttttc
     8281 catgatttag caagatgtcc ttaaaggtat agtgttttaa aaacataaaa tatacattaa
     8341 taaattataa gattttagta gagttaaaaa aaatctatga tatgtcaaaa aaatctataa
     8401 tttgtttttg aaaacctata caaaaaatga tataattagt tcataataga aatataaaaa
     8461 ataataagaa gaggtgagtt tagtatggca cataaaaagg tggcaaaaat aatagcaact
     8521 gctatagtag taaataacct ctccagtagc gtagttttag gcaaagaagt aaatttaata
     8581 gataaaaaca aagataattc agttataaat caaaatataa gtcatagtaa aagacataat
     8641 aatattttta gaatagaaaa aacagtagga aatacagagg aattcttaaa aataataaat
     8701 gatgggaact tagaagagat aaaattatca agggacattg accttagcaa tttaatttct
     8761 agtggagtag atattaagag ttcaaatgtt gtaatagatg gtcaaggata tagtatctat
     8821 gttcctaaat tagctgaaaa tcttaataga gattttttta cactacaggg agatgggatt
     8881 gtgctaaaaa atttaaatat agtatgtaag gatgatgaag atttaaacaa taataacctt
     8941 attactatat caggagatga cataacttta gaaaatgttt tcgtagaatc taactttaat
     9001 agagcagtaa acattttaga gggaaataat attcatatta atgattgtaa aatagtaaat
     9061 aatcaagaaa agggtaatgg attaaaggtt gaaaatggaa tagtaacctt aaataatctt
     9121 gatttacaaa ataaatctgg agttggactt gacattttag gaaaagattc aaaggtttat
     9181 ttaaaaggag atataaaaat aaattctcca attgaaataa gggggcaatt tagagacggt
     9241 ggaattttaa attgtgacaa taatcagtta atccacgaaa aaagagtctt tggatatact
     9301 tattataatg ttgcaaagga aacagagtta gttagaaata aagatgagtt tttagaagca
     9361 atagataata atatagttaa gaatataaaa ttaatggaca atattgattt aagaggatat
     9421 gaatcagaat attataaaat ttttgaaaaa gaaataaagg ttgataaaaa taattatcat
     9481 ataacaatgt aaagtttaaa ggtatctaaa cggagtcaaa tctcagcgtt tagatacctt
     9541 ttatagtata atatataatt tgctatttaa aagtgttaat tattaagatt agtattaact
     9601 ttcaatcaat taaaaaattt gcaacattag tattatatta tttaacaact atatttacaa
     9661 gtctaccttt tataactatt acttttacaa ctgtttttcc ttctgttgaa gctataattt
     9721 tctcatcagc taaagcagcg gcttttattc cttcttcatc taaatcagaa gaaaccatta
     9781 ttttgttttt gatttttccg ttaacttgga tagctatttc aacttcgtct ttaactaaag
     9841 ctttagggtc aaattttggc catgcttcat tgaagattga gtatgaattt ccaagtaagt
     9901 tccattgttc ttcactgaag tgaggtgcaa atggtgctaa taatcttaaa taatcagaaa
     9961 ctacatcttt taagaaatca agattcattt ctttctcttg agtatattta gatagagcat
    10021 ttatgaattc catcattcta gctattgctg tattaaattg aagtttatct gtatcatctg
    10081 taacagattt tatagtatta tgtctccaat agttaagctc tttttcagcc ttatccattg
    10141 tagttttatt attttcacct tttgagattg cttctctagc agtatctata attctttcaa
    10201 ttctttcaac gaatctattt acagatttga ttccatcatc actccaagct ccaccttcag
    10261 tgtaagcaaa accaaacatt aggtacattc taaatacatc tgcaccatat tcttttatat
    10321 aatcatcagg agatattgtg tttccttttg atttactcat ttttaatcca tctggtccta
    10381 agattaatcc ttggtgagtt aatgaagtaa atggttcatc aaagtttagg tatcccatat
    10441 ctcttagggc tttagttatg aatcttgcgt aaagaagatg catacaagca tgttcaggtc
    10501 caccaacgta tttatcaact ggtaacattt tgttgattaa ttcaggatta aatggagcct
    10561 cagtattttt attatcagga tatcttaagt aataccatga tgaacatacg aaagtatcta
    10621 aagtatcagt ttctctctta gctggttttc cacagtgagg gcaagttgtg ttaacaaatg
    10681 cttcgctctt agctagtgga gattttccat ctggagcaaa ttcaacatca tatggtaatt
    10741 caactggtaa ttgactttca ggtacaggaa ctataccaca ttcttcacag taaactacag
    10801 gaattggagc tccccagtat ctttgtcttg aaactaacca gtctcttaat ctaaagttaa
    10861 ctttcttttc tccaaggccc attgaagcta gtttttcaac tattttttct ttagcctcat
    10921 ctgtagttaa tccatcaaat tcacctgagt taactaatat accgtgctcg cagaatggaa
    10981 ggttagtttc actaccatct ttagcttcta taactctatt tattggaagg ttgaattttt
    11041 cagcaaaagc aaaatctctt tcatcatgag caggaacagc cataacagct ccagttccgt
    11101 aagttgctag aacgtagtct ccaacccata taggaacttc tttgcctgtt aatggatgaa
    11161 tagcatatgc accagtgaat acaccagttt tttctcttga aatagattgt ctttctatat
    11221 cagattgttt tttagcttct tctttataat tttcaacagc ttctttttgt tcagctgaaa
    11281 ctatttcgtc tactaatgga ttttcaggag ctaaaacaac atagctgact ccgcataaag
    11341 tatcaactct tgttgtgaat acatcaaagt ttaaatcgct atctttaact ttaaagttaa
    11401 cttgagaacc agttgatctt cctatccagt gtttttgcat agaaactgtt ttttctggcc
    11461 aatctaaacc atctaattta tctaataatt catcagcata atcagttatt ttaaagaacc
    11521 attgagtaag atctttttta gtaacttctg ttgaacatct ttcacaagct ccatcaacta
    11581 cttgctcgtt agcaagaact gtttggcatg atggacacca gtttacagga gctttttttc
    11641 tataagctaa tcctttctcg tataatttta ggaatagcca ttgagtccat ttataatatt
    11701 caggtgagca agttacaact tcgttttccc agttaaacat agctcccata gcttttaatt
    11761 gttcttccat tttagctata ttttttattg tagaatcttg aggatgtatt cctgttttta
    11821 tagcaaagtt ttcagcaggt aaaccgaaag catcaaatcc cattggttgg aatacattat
    11881 atccttgcat tcttttaaat ctagcccatg aatcaactgg tccatagtta aaccagtgac
    11941 ctgcatgtaa ttggctacct gaagggtatg agaacatttc taaaacgtaa agtttctcta
    12001 cttctttatt tggatcaaat ttataaaggc ctgattcagc ccatttatct tgccatttct
    12061 tatctatggc agtactatag ttacccatta actaatcctc ctctaagtct atataattaa
    12121 tattatttgc tagaatcaaa taaaaacaaa taaaaaacct tccatctctt aaaatttaag
    12181 agacgaaagg atattcgcgg taccactctt attaggaaaa tttcttttcc tcactcgatt
    12241 ttataacggg tttttcccgt atgtgctttt tttcacacat atgctcatag gcaagttcat
    12301 attttcatac cattgtgtca cagcaaacca caactctctt taggctatga ggtaatatta
    12361 ctactcctaa tcaaagcttt taatgattca gtttttatat attatatatc aaaagtttaa
    12421 aataagtcaa ttaaagtttc taataaatac ctaggatgaa aaaaatataa ttacaaaaaa
    12481 taaggttgaa caagtttcaa taaaaaatag aaagaggtaa ttattcatgg gaaaaaaact
    12541 aaaatcagca acaaataatc aatgggtatc aacaactgta gatcctaaca tggttcctgc
    12601 aaaggataga cagcctaaaa ctgcaggaga taatcaatgg acatcaactt ctaaaaatcc
    12661 aatgaaatag taagctttat atacacttag tttagagaga ataatctttt aaactaagtg
    12721 tatatttttt tgttttgtta gttttttatt ccaagggtaa aacatagcat tatattagaa
    12781 attagaattt gatgcataat taaattgatt gcaattatat attttaaaaa
//
LOCUS       DAEVGI010000065        12812 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEVGI010000065 DAEVGI010000000
VERSION     DAEVGI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.065"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(169..1359)
                     /locus_tag="K8M77_001506"
     CDS             complement(169..1359)
                     /locus_tag="K8M77_001506"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI7086003.1"
                     /translation="MSKYKRIFTIVIDSLGIGAMNDSEKYGDVNVDTLGHIAESVDTF
                     NIPNLQKMGIANLHPIKHVAPVENPIGYQAKMAEASVGKDTMTGHWEMMGLHITKPFK
                     TFTDTGFPQELLDELTARTGHKIVGNKSASGTEILDELGEHQIATGDMIVYTSADSVL
                     QICGQEETFGLEELYRCCEIARELTLKDEWKVGRIIARPYLGTKKGEFKRTSNRHDYA
                     LKPYGRTVLNELKDNNFDVISVGKIKDIFDGEGITEGNKSKSSVHGMEQTLEIMDRDF
                     TGFCFINLVDFDALWGHRRNPQGYAEELEKFDVNLGKVLEKLHEDDLLIITADHGNDP
                     TYTGTDHTREYVPFLAYSPSMKGHGQLETPKTFATIGATIADNFGLKMPEGTIGESVL
                     NKLV"
     gene            complement(1537..2322)
                     /gene="udp"
                     /locus_tag="K8M77_001507"
     CDS             complement(1537..2322)
                     /gene="udp"
                     /locus_tag="K8M77_001507"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBI7086004.1"
                     /translation="MIYTQGSDKQFHIDVAPGEVGKYVIMPGDPKRCAKIAKYLDNAK
                     LIADKREYVTYTGYLDGEKVSVTSTGIGGPSAAIALEELVKCGADTFIRVGTCGGIDL
                     NVKGGDLIIATGSIRAEGTSREYAPIEFPAVANLDVINALVKGAKELKVPYHTGVVQS
                     KDSFYGQHSPETKPVSYDLLNKWEAWKRCGCLGSEMEGAALFIVGSYLRVRVGAVFLT
                     IANQERQKEGLDNPQVHDTELAIKAGIEGIRNLIKEDKMKNNA"
     gene            2556..3287
                     /locus_tag="K8M77_001508"
     CDS             2556..3287
                     /locus_tag="K8M77_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7086005.1"
                     /translation="MEILTVNKHKELLYVTVYDKLFKMINEGTFPENSRLPSEPELAK
                     RLGVSRSTLRQALALLQDDGLIKNIRGKGNYIIKENKTDTTGLEKIGHPVYKCLDLET
                     NNIELDFKIDPPSDYYKKILGDSSVATVCIDRWYLDDNLAIAYSYTIIPIEVISKFNI
                     DLSDKNNLLKFIEEESYKECSKIKSTIKFTTSGNFVTKRHPIPDESQFYLIEEAIYSN
                     SELPVIFNKHYLPMKHSTIKVNPMK"
     gene            complement(3310..3618)
                     /locus_tag="K8M77_001509"
                     /pseudo
     CDS             complement(3310..3618)
                     /locus_tag="K8M77_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480088.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            3821..4741
                     /locus_tag="K8M77_001510"
     CDS             3821..4741
                     /locus_tag="K8M77_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI7086006.1"
                     /translation="MKKIKHKGFLIALVSALIISSSFIACDMKGNKSNVENEKANLEN
                     NTQKDEASSNKENPTDNTSKKSNSNAEATKNRFEGLKTTSEDMNIPVLCYHDVTPNNP
                     NNNELLVNPEKFKEQLQYLKDNNYTPITLDELYDYLRNNKPIPEKSVVITLDDGYKGN
                     YEYAYPLLKEFKFPATIFVISNYVGAQDYMTADQLKEMSNNGIEIESHTFKHDDLSTL
                     DEAKQLETLKDSKVALEKIIGKPVDFVAYPFGRYNSSTRVAAEKAGYKLGFNLNGNFT
                     DRKDNNFNMDRIYVSNNDSLQKFESRLTGK"
     gene            complement(4904..5302)
                     /locus_tag="K8M77_001511"
     CDS             complement(4904..5302)
                     /locus_tag="K8M77_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI7086007.1"
                     /translation="MENIINYIKWGIVSLGTLFTWIFGAWDIPLITLLVFIFLDYLTG
                     VIKGCKSKELCSNIGLRGIAKKGLILVVLLVAVMLDRLLGNGTWMFRTMIAYFYITNE
                     GISILENCSALGVPIPEKLKQALKQLNNKK"
     gene            5524..5922
                     /locus_tag="K8M77_001512"
     CDS             5524..5922
                     /locus_tag="K8M77_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HBI7086008.1"
                     /translation="MKQNGNEVLLKLQQGELDAVLVYRKLAELSSSEEEKNVLLSIAA
                     DEGRHASIIREYSKEILKPCNKSSEEIEAAYKNLGKDKVFEMLINAEINGGPVYEKLG
                     EEFPRLKEIAKDEIKHGNLLKGLIEKSNLN"
     gene            complement(6006..7787)
                     /locus_tag="K8M77_001513"
     CDS             complement(6006..7787)
                     /locus_tag="K8M77_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086009.1"
                     /translation="MNKFKSVVKKISPFVGKYKWAFLGAILFIISAAVFTAIAPRTEG
                     LIITQLTKDFEGIIRGVSGASVNFNYIFKVLILLFLVYAGGTGSTLIASFLLTNAIQN
                     TMKNIRNEVEKKINRLPIRYFDGNSYGDVLSRITNDVDTISNALQQSFVQVINAFLSV
                     GLALIMMYSINIKLALIATLIIPLSILITKIIVSKSQKLFNSQQKALGNLNGKVQEMY
                     TGFNELKLYGKEDDALNEFVKVNEELRETGFKAQFISGIMSPLISLVTYLGIAVVGVV
                     GSIIAIGGGITVGNLQAFIRYIWQINQPLSQVTQLSSAIQSAVAAANRVFEFLEEEEE
                     VKDLNNAIKIENPKGDVTFEHVKFGYKEDKPLIKDLSVDVKSGQMVAIVGKTGAGKTT
                     LINLLMRFYDVQGGSIKIDGVDIRDMKREDLRAMFGMVLQDTWLFNGTIYDNIRYGRF
                     DATKEEIIEAAKVANVHHFIKTLPGGYNMLLNEEASNVSQGEKQLLTIARAILKDPAI
                     LILDEATSSVDTRLELLLQKAMRNIMKDRTSFVIAHRLSTIRSADLILVMNDGNIIEQ
                     GTHEELMKQGGYYESLYNSQFASKEVN"
     gene            complement(7780..9513)
                     /locus_tag="K8M77_001514"
     CDS             complement(7780..9513)
                     /locus_tag="K8M77_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086010.1"
                     /translation="MKLILKYLKNYKSIFILNVISVFGFALVELGIPTIMAKVIDIGI
                     INNDISYIKKMGLVIVVISIVGVMGTILLGYCSSKISIGITKDIRNDIFKKAQEFSHT
                     EYDEFGVSSMITRTTNDPFVILQFTNTLLKMALITPVMFTISLVMILKTSLSLSLVLA
                     VAIPLIIFGVIIIAKTSEPLSEKLQKRLDKLNRISRENLTGIRVIRAFGNEKYEEKRF
                     DETNESYAEVAKKLNKLMAISQPLFFFLLNIAIIVIFWISSKMINVGTLQVGQLVAFI
                     EYLFHAMFSIMLFSMVFVMYPKAQVSANRIMEVLNTEPIIKSPKNGIKDTENKGTIEF
                     ENVSFTYPNGEEPVIKDVSFSGKKGEVIAFIGSTGSGKSTLINLIPRFYDVTKGSIKI
                     DGVDIRDYDLKYLRKKIGFIPQKTLLFTGSIANNIKFGKKDAEYKEVEDSAKIAQAYE
                     FISNKPNKFDELITEGGSNVSGGQKQRLSIARAIVRKPDIYIFDDSFSALDFKTDAIL
                     RKELRSETKDAIVLIVAQRIGSIMDADKIIVLNEGEVVGIGTHKELIKNCPIYHEIAT
                     SQLTEEELANE"
     gene            complement(9903..11696)
                     /locus_tag="K8M77_001515"
     CDS             complement(9903..11696)
                     /locus_tag="K8M77_001515"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBI7086011.1"
                     /translation="MKLKTHDDRLTLTNGSYHALVHARKPKGIENKSAYLIGTGIGAL
                     AAGCFLIRDAHMDGSKITFFEQLDLPGGSLDGEVLQNLGYVARGGREMGHHFEVLWDL
                     FSSLPSTEDPNMTVLDHFFYTNYDDPNYSNCRITHKNGERYDNAKFNLGQDLAKELAE
                     FVLISDEELQDKAIDEIFSEELLNTDFWTLWRTMFAFENWHSALEMKLYMNRFIHHVG
                     GLPTLSALQFSRHDQYTSFVKPMVKFLKENGAKFEYGVHVENVEFDISHDKKVAKKIV
                     AKDKNGKDISVNLSENDLVFITNGSMTEGSGYGDDNTPAPFNKEPQGCWELWRNIAKQ
                     SDEFGHPDKFCTDTEKSNWESCTVTCHDERVPKYIEKITKRSPYGGKTVTGGIVTAVD
                     SSWLMSWTINRQEQYYGQPEKDVVVWVYGLFSDVDGDYIKKPMRDCTGKEITKEWLFH
                     IGVPVDEIEELAESCTAIPVMMPFITSQFMPREFGDRPYVVPKNAVNFAFLGQFAETL
                     DDPGRDTVFTIEYSGRTAMEAVYVLTGVEKGVPEVYASRYDIRYLLNAGVCLLDGEKP
                     KVHIPFLLKRKLMKQISGTDIEKLLKEYGII"
     gene            complement(12101..12670)
                     /locus_tag="K8M77_001516"
     CDS             complement(12101..12670)
                     /locus_tag="K8M77_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI7086012.1"
                     /translation="MAQQYTKKMIRSVFIELLNERPLNKITVKDIALSCGINRNTFYY
                     YYSDMYELLSELFQTELQNVIDEYNDTMSWEESFVLATRFALGNKKVIYHVYNSMQRE
                     ELVNYIYNVSENMMERYVKKLSQGINASEYDMKLIATFYQCALTEMVIQWISSGMKEE
                     PDATIRRIGELFDGNIKLSLQRSEKLNRI"
BASE COUNT         4298 a         2139 c         1516 g         4859 t
ORIGIN      
        1 gaagcgaagc gagtttcgaa gctttagtaa tatcttcgga gtaataatta taaattctta
       61 gcgaaatcta gtaattctct gaatatgttt gtaaggttta ttatctttat aagaaaagct
      121 atatcaaata taaaatttaa tatagctttt atatattctt acaaattatt aaactaattt
      181 atttagaact gattcaccaa ttgttccttc tggcattttt aatccaaagt tatcagcaat
      241 tgttgctcca atagttgcaa atgtttttgg agtttctaat tgtccatgac ctttcattga
      301 tggtgaataa gctaagaatg gtacatactc acgagtgtgg tctgttcctg tgtatgttgg
      361 gtcattacca tggtcagctg ttattattaa taaatcatct tcatgtaatt tttctaatac
      421 ttttcctaag ttaacgtcga atttttctaa ttcttctgca taaccttgtg gatttcttct
      481 atgtccccat aaagcatcaa agtcaactaa gttaataaag cagaatccag taaagtctct
      541 atccattatt tctaatgttt gttccatacc atgaactgaa ctcttagatt tatttccttc
      601 tgttattcct tctccatcaa atatatcttt tattttacca actgaaataa catcaaagtt
      661 gttgtctttt aattcattaa gaactgttct tccatatggt ttaagagcat agtcatgtct
      721 attgcttgtt cttttgaatt cacctttttt agttcctaag taaggtcttg ctatgattct
      781 tccaactttc cattcatctt taagagttaa ttctcttgcg atttcacaac atctatataa
      841 ttcttctaag ccaaaagttt cttcttgacc acaaatttgt aaaactgaat ctgctgatgt
      901 ataaactatc atatctccag ttgcaatttg atgttctcct aattcatcta ggatttctgt
      961 tccactagca ctcttgtttc ctactatttt atgaccagtt cttgctgtta actcatctaa
     1021 taattcttgt gggaatccag tatctgtgaa agttttaaat ggcttagtta tgtgtaagcc
     1081 catcatttcc cagtgtcctg tcattgtatc tttaccaaca cttgcttcag ccattttagc
     1141 ttggtatcct attggattct caactggagc tacgtgtttt attgggtgta agtttgctat
     1201 acccattttt tgaagattag gtatattaaa tgtgtctact gattcagcaa tgtgtcctaa
     1261 tgtatcaaca ttaacatctc catatttttc tgaatcattc attgcaccta ttcctaatga
     1321 atctattact attgtgaaaa ttcttttgta tttgctcatt gttaaaaccc cctgagtgta
     1381 tttccgtatt tattataatt tttaagattt tatcttatgc atcaaaattt aatttttcta
     1441 ataattagta attctaaagc taaatcaaaa agattttact aagaactact aagctattag
     1501 ataaatttaa ctaaaattta taggtctctt aagatcctat gcattatttt tcattttatc
     1561 ttctttaatt aagtttctta ttccttctat tcccgctttt attgcaagtt ctgtatcatg
     1621 tacttgagga ttatcaagtc cttctttttg tctttcttgg ttagctatag ttaggaaaac
     1681 agctccaact cttactctta aatagcttcc tactataaat aatgctgcac cttccatttc
     1741 tgatcctaaa catccacatc ttttccatgc ttcccatttg tttaataagt catagcttac
     1801 aggtttagtt tctggtgagt gttgtccata aaaagagtcc tttgattgaa ccacaccagt
     1861 atggtaagga acttttaatt cttttgctcc tttaactaaa gcatttatta catctaagtt
     1921 tgctactgct gggaattcta ttggagcata ttctcttgaa gtaccttctg ctcttataga
     1981 tccagttgct attataagat ctccaccttt aacgtttaaa tctattcctc cacaagtccc
     2041 tactctgatg aaagtgtctg ctccacattt aactaattct tctaatgcta ttgcagctga
     2101 tgggccacca attccagttg aggtaacgct tactttttct ccatctaaat atccagtata
     2161 tgtaacatat tctcttttat ctgctattaa cttagcattg tctaagtatt ttgctatttt
     2221 agcacatctt tttggatctc caggcattat tacatatttt ccaacttctc ctggtgctac
     2281 gtctatatga aattgtttgt ctgaaccttg tgtataaatc atttacattt cctcctaaat
     2341 ccttgtttcc ttataacaat tttaaacttg tctacttagt tgtctatttt atatttatat
     2401 aatactacac tttctagaaa ttatcaaacg tttacagaaa attttttaaa ttatttgttg
     2461 gttattaata taaaatattt tataatttaa gtaaataata ctttgtccta tccaacttgt
     2521 ttgtaacttg tctattttta ggaaggtgat tataaatgga aatattaact gtaaataaac
     2581 ataaagaact cttatatgtt accgtatacg ataaactttt taagatgatt aacgaaggaa
     2641 ctttccctga aaatagcaga cttccttctg aaccggaact agctaaacga ttaggtgtta
     2701 gtagaagtac acttagacaa gctttagccc tgcttcaaga tgatggatta ataaaaaaca
     2761 ttagaggtaa aggaaattac ataattaaag aaaataaaac tgatacaaca ggtttagaaa
     2821 aaataggaca tccagtatat aagtgtttag atttagagac aaataatatt gaattagatt
     2881 ttaaaataga tccaccctca gattattata aaaagatatt aggagatagt tctgtagcaa
     2941 cggtttgtat tgatagatgg tatttagatg ataatttagc tatagcttat tcttacacaa
     3001 taatacctat tgaagtaatt tcaaaattta atatagatct atcagataaa aataatttgt
     3061 tgaaattcat tgaagaagaa tcttataaag aatgctcaaa gataaaaagt actattaaat
     3121 tcactacatc tggtaacttt gtaactaagc gtcacccaat tccagatgaa agtcaatttt
     3181 atttaattga ggaagctata tattctaatt cagaacttcc agttattttt aataagcatt
     3241 atctcccaat gaaacacagc accataaaag taaatcctat gaaataatat ttcataggat
     3301 ttatatctat taaaatgctg taaaaccacc atctactggc ataaatattc cagttacatt
     3361 acttgcttct ggtgatgcta agaataatat gacatcggca acttcttctg ctcttgccac
     3421 tctattttgc ggagaattaa cttttcctga tactattccc gcttctactt taactcctgc
     3481 cttttcagta gcttcccttt tatttttcaa aaattctaaa gcttcattat acattggagt
     3541 atctgtagtt cctagattca ctgcatttac tcttatacca tatctagcat aatccaatac
     3601 catatttcta gtaagtcctt ttgttagtga atatttctaa taattagacc aataataaac
     3661 tttaaaatat ttaattataa atttattaaa atgattattt ttttcaatta tttgagaaat
     3721 acctagaggt tatcgtaata ataaagtaac ctaaatttta ctttttttat gatatactaa
     3781 attattgtaa acaaaattta ttagtaagga gatttaaaag atgaaaaaaa taaaacacaa
     3841 aggatttcta atagctctag tttctgcact aattataagt tcatctttca ttgcttgtga
     3901 catgaaaggt aataaaagca atgtggaaaa tgaaaaagca aatttagaaa ataatactca
     3961 aaaagatgaa gcttctagca ataaggaaaa tcctacagat aatactagta aaaaatcaaa
     4021 ttcaaatgct gaagctacta agaatagatt tgaagggtta aaaactactt ctgaagatat
     4081 gaatattcct gttttatgtt accatgatgt aactccaaac aatccaaata acaatgaact
     4141 tttagtaaac cctgaaaaat tcaaagaaca acttcaatat ttaaaggata ataattatac
     4201 acctataact ttagatgaac tttatgatta tctaagaaat aataaaccta ttcctgaaaa
     4261 atctgttgtt ataactcttg atgatggata taaaggaaac tatgaatatg cttatccttt
     4321 actaaaagaa ttcaaatttc ctgctacaat atttgtgatt tcaaattatg ttggagcaca
     4381 ggattatatg actgctgatc aattaaaaga aatgtctaat aacggtatag aaatagaaag
     4441 tcatactttt aaacatgatg acttatctac attagatgaa gctaaacaac ttgaaactct
     4501 aaaagattct aaagtagctt tagaaaaaat cattggaaaa cctgtagatt ttgttgctta
     4561 tccttttgga agatataact ctagtactag agttgcagca gaaaaagctg gatataaatt
     4621 aggttttaat ttaaatggaa actttactga cagaaaagac aataatttta acatggatag
     4681 aatttatgtt agtaataatg attccttaca aaaattcgaa agtagattaa ctggcaaata
     4741 ggtttaaaat aagctgtata aaataactaa ttcctaaaac ttataaataa tataaagaaa
     4801 ctacttttat aaattcttaa tgaaatttag taattctaaa aatttattta taagttttat
     4861 atttaagttt ttatacagct tataaaaaat attttattta atattatttt ttattattta
     4921 actgtttaag agcttgtttt aatttttcag gtataggtac tcctaaggct gagcaatttt
     4981 ctaatatact tataccttca tttgttatat agaaataagc tatcattgtt ctaaacatcc
     5041 aagttccatt gcctaataac ctatctagca taacagcaac taaaagaaca actaatataa
     5101 gacctttttt agctattccc cttaatccta tattcgaaca taattcctta cttttacacc
     5161 ccttaattac cccagttaaa taatctaaaa aaataaatac taataaagtt attagtggaa
     5221 tatcccatgc accaaatatc caagtgaata aagttcctaa ggatacaatc ccccacttta
     5281 tataatttat tatattctcc ataataacta tacctcttaa acttaattta acttttgtta
     5341 cctatatcaa tatataatat ttataattct ctttaattgt ccttattttt caaatataat
     5401 ttcactaata atatttaatt actaaaaaaa tttgacatat tgttataaaa gctttaaaat
     5461 aaaattatta aatttaattt tatacaatta aatttaataa aatgaattta ggagggtttt
     5521 attatgaaac aaaatggaaa tgaagtgtta ttaaaattac aacaaggaga attagatgca
     5581 gttcttgttt atagaaaact tgctgaactc tctagctctg aagaagaaaa aaatgtttta
     5641 ttaagcatag cagcagatga aggaagacat gcctcaataa taagagaata ttcaaaagag
     5701 attttaaaac cttgcaataa atcatctgaa gaaatagaag ctgcttataa aaacttaggt
     5761 aaagataaag tttttgaaat gcttataaat gctgaaatta atggtggccc agtatatgaa
     5821 aagcttggag aagaattccc tagacttaaa gaaatagcta aggatgaaat aaaacatggt
     5881 aatttattaa aaggattaat agaaaaaagc aatctaaact aatttagatt gctttttcta
     5941 tagtgttaaa tatatatcca ggaatccata ttcccctaaa atatatcata gtatttaaag
     6001 tttatttaat ttacttcttt actagcaaac tgactattat aaagactttc gtaataacca
     6061 ccttgcttca ttaactcttc atgagttccc tgttcaatta tattaccatc attcattact
     6121 aatattaaat ctgcacttct tatagttgaa agcctgtgag ctataacaaa actagttcta
     6181 tccttcatta tatttctcat ggccttttga agtaataatt ctaatctagt atcaactgaa
     6241 cttgtagcct catctagtat taatatagct ggatctttta aaattgctct agctatggtt
     6301 aaaagttgct tttctccctg agatacattt gaagcctctt catttaataa catattatat
     6361 ccacctggta atgtttttat aaaatgatga acatttgcaa cctttgctgc ctctattatt
     6421 tcttctttag tggcatcaaa tctaccatac ctaatattat cataaatagt tccattaaat
     6481 agccaagtgt cttgaagaac cataccaaac atagctctta aatcttcacg tttcatatct
     6541 ctaatatcaa caccgtctat tttaattgaa cctccttgaa catcataaaa tctcattaaa
     6601 agattaataa gagtagtttt acctgctcca gtttttccaa caatggcaac catctgacca
     6661 cttttgacat ctacgcttaa atcttttata agtggcttat cttccttata tccaaattta
     6721 acatgttcaa aagttacatc tccctttgga ttctcaattt ttatagcatt attaagatct
     6781 ttaacctctt cttcttcctc taagaattca aaaaccctat tagcagcagc cactgctgat
     6841 tgtattgctg atgataattg agttacttga gataaaggct gatttatttg ccaaatataa
     6901 cgtataaatg cttgaagatt accaacggta attccaccac ctatagctat tatagaacct
     6961 actaccccta ctacagctat tcctaaataa gtaacaagac ttattaaagg tgacattata
     7021 ccagatataa attgagcttt aaaccctgtt tctcttaact cttcatttac ctttacaaat
     7081 tcatttaaag catcatcttc ttttccatat aactttaatt cattaaagcc tgtgtacatt
     7141 tcttgtactt ttccattaag atttcctaag gccttttgtt gactattaaa taatttctga
     7201 gatttagaaa ctattatctt tgtaattaaa atacttaaag gtattattaa ggttgcaatt
     7261 aatgctagct taatatttat ggaatacatc attattaaag ctaatcctac agataaaaat
     7321 gcatttatta cctgtacaaa actttgctgc aatgcattag agattgtatc tacatcatta
     7381 gttattctac ttaatacatc tccataacta ttaccatcaa aatatcttat tggaagtcta
     7441 tttatttttt tctcaacttc atttctaata tttttcatgg tattttgaat tgcattagtt
     7501 aataagaaac ttgctataag agttgatcct gtaccacctg cataaactaa aaataataaa
     7561 attaatacct taaaaatata attaaagtta actgaagctc cagaaactcc tcttataatt
     7621 ccctcaaaat ccttagttaa ttgagttatt attaatcctt ctgttcttgg tgctatggca
     7681 gtgaaaacag ctgctgaaat tataaataaa atagctccta aaaaagccca cttatactta
     7741 ccaacaaaag gagaaatctt ttttactact gatttaaatt tattcatttg ctaactcctc
     7801 ctctgtaagt tgtgatgttg ctatttcatg ataaataggg caatttttta ttaattcctt
     7861 gtgagttcct attccaacta cttccccttc atttagaact ataatcttat ctgcatccat
     7921 tatagaacct attctttgag ccactattaa tacaatggca tcctttgttt cagatctaag
     7981 ttcttttctt aagattgcat ctgttttgaa gtctaaagct gagaaactat catcaaaaat
     8041 gtatatatca ggctttctaa caattgccct agctatggaa agtctttgct tttgtccacc
     8101 agatacgtta gaaccaccct ctgttattaa ctcatcaaat ttattaggct tatttgaaat
     8161 aaattcatag gcctgtgcaa ttttagctga atcctcaacc tctttatact ctgcatcttt
     8221 cttaccaaat tttatattat ttgctatgct accagtaaat aaaagagtct tttgaggaat
     8281 gaaacctatt tttttacgta gatattttaa atcataatct cttatatcta ctccatctat
     8341 ttttatactt cccttagtaa catcataaaa ccttggaatt aagtttataa gtgtactctt
     8401 accactacct gtacttccta taaaggcaat aacttctccc tttttaccac taaaggatac
     8461 atctttaatt acaggctcct ctccatttgg ataagtaaaa gatacatttt caaactctat
     8521 tgttcccttg ttttctgtat cctttattcc atttttagga ctttttatta ttggttctgt
     8581 atttaaaact tccataattc tattagcaga aacctgtgct tttggataca ttacaaaaac
     8641 catagaaaat agcattattg aaaacattgc atggaataga tattcaataa aggcaactaa
     8701 ttgaccaact tgaagtgttc ctacatttat catcttacta gatatccaaa agataactat
     8761 aatagcgata ttaagtaaga aaaagaaaag tggttgagat attgccataa gtttatttaa
     8821 ctttttagcc acctctgcat aggattcatt tgtttcatca aatctcttct cctcatactt
     8881 ttcatttcca aaggctctta ttacccttat accagttaaa ttttctctag atattctatt
     8941 taacttatct aacctttttt gtaacttttc tgataaaggt tcagaagttt tagctattat
     9001 tataacccca aaaattatta aaggaattgc cactgctaaa actaaagata aggaaaggct
     9061 tgttttaagt atcattacta aacttatagt aaacataact ggtgtgatta aagccatttt
     9121 tagaagtgtg tttgtaaatt gtaaaataac aaaaggatcg tttgtagttc ttgttatcat
     9181 agaagaaact ccaaactcat catactctgt atgggaaaac tcttgagctt ttttaaatat
     9241 atcatttcta atatccttag ttattccaat tgaaatctta gatgaacaat atccaagtaa
     9301 tattgttccc ataacaccaa ctatagaaat aaccactatt actaggccca tttttttgat
     9361 ataagaaata tcattattta ttattccaat atctataact tttgccataa tagttggaat
     9421 tccaagctca actaaagcaa acccaaatac tgatataaca tttaagataa aaattgattt
     9481 gtagtttttt aaatatttta gtattagttt cattgctata tacccctccc tttctcaaaa
     9541 ttttttataa tgtaattatt aattatacca aaattaactc tttctcataa gtgtattttt
     9601 agttaattat atgttaattt tgttcatatt ctttaattaa tatatttaat cttatgttta
     9661 taaggtctat taaatattat tatactaaaa atatattttt aatatttata gtataatatt
     9721 ttaaatttta tccatattga tttatatttt aaaaatattt taaataccaa aataaaaaat
     9781 agaccataat aactgccccc aatttttaaa aggttgctat ttatcatggt ctattaaata
     9841 tacttactat atttttattt taatctaaga ttattattct tttaatcgta agcacttttt
     9901 acttagataa ttccatattc ttttaataat ttttctatgt cagttcctga tatttgcttc
     9961 attaactttc tctttaataa aaatggtata tgaacttttg gtttttcacc atctaataaa
    10021 caaactccag catttaataa gtaacgaata tcatatcttg atgcatatac ttctggtact
    10081 cccttttcaa ctccagttaa tacatatact gcttccatag ctgtacgtcc tgagtattca
    10141 atagtaaata ctgtatctct tcctggatcg tctaaagttt ctgcaaattg tccaaggaag
    10201 gcaaagttaa ctgcattctt aggaacaacg taaggtctgt ctccaaattc acgaggcata
    10261 aattgtgaag ttataaatgg catcattaca ggaattgctg tacaagattc agcaagttct
    10321 tcaatttcat caactggaac gcctatatgg aataaccatt cctttgtaat ttccttacca
    10381 gtacaatctc tcataggttt cttaatataa tcaccatcaa catctgagaa tagtccatat
    10441 acccaaacta ctacgtcttt ttctggttga ccataatatt gttcttgacg atttatagtc
    10501 caactcatta accatgaaga atccacagct gtaactattc ctcctgttac agtttttcct
    10561 ccatatggtg aacgtttagt tattttttca atatattttg gtacacgttc atcatgacaa
    10621 gttactgtac aagattccca gtttgatttt tcagtatctg tacagaattt atctggatgt
    10681 ccaaattcat ctgattgttt agcaatattt ctccataact cccagcatcc ttgtggttct
    10741 ttattaaatg gagcaggagt attatcatct ccatatcctg aaccttctgt cattgaacca
    10801 ttagttataa atactaaatc attttcactt aagtttacag aaatatcttt tccgttttta
    10861 tccttagcaa ctattttttt agccaccttt ttatcatggc ttatgtcaaa ttctacattt
    10921 tcaacatgta ctccatattc aaacttagcc ccattttctt ttaagaattt aaccattggt
    10981 tttacaaaag atgtatattg atcatgtctt gagaattgca gtgctgaaag tgttggtaat
    11041 ccaccaacat ggtgtataaa tctattcata tataatttca tttctaaagc actatgccag
    11101 ttttcaaagg caaacatagt tctccataat gtccaaaagt ctgtatttaa aagctcttca
    11161 gagaatattt catcgattgc tttgtcttga agttcttcat ctgatataag aacaaattct
    11221 gctaattcct tagctaaatc ttgtcctaaa ttaaatttag cgttgtcata tctttctcca
    11281 tttttatgag ttatacggca attgctatag tttggatcat cataatttgt gtagaagaaa
    11341 tgatctaata ctgtcatatt tggatcttca gtagatggaa gtgaactaaa taaatcccat
    11401 aaaacttcaa agtgatgtcc catttcacgt ccaccacgag ctacataacc tagattttga
    11461 agtacttctc catctaaaga gcctcctgga agatctaatt gttcaaaaaa tgttattttg
    11521 ctaccatcca tatgtgcatc tctaattaaa aaacatcctg ctgctaacgc acctattcca
    11581 gtaccaatta aatatgcact tttattttca attcctttag gttttctagc gtgtactaat
    11641 gcatgatagc ttccatttgt taaagtaagt ctgtcatcat gtgttttaag tttcataaaa
    11701 tccccttctt tcttaaaact agctataagt tttcaaaata aatcttagcc ctttttaaat
    11761 caatccttta ttaataagtc ataatcttat aagtataaga ttttatttac taagtgtaag
    11821 ttttaaaaat aactctaact tcctttaaat ctataatata accttttacc atatatttca
    11881 ttagataaat aaggggagtg ttcaaagttc aaacacttaa acacatttga ctaaaaatta
    11941 ggcatatcta actctgtgcc taattttata aatattttat aatctaattt aaatattttt
    12001 agctatttct aggttttctc tgcgattatt tgtgatttca tttattttaa ctattaaggg
    12061 atttaatact taatttatta attttctatg cttgcccttg ctaaattctg ttgagctttt
    12121 cgctacgttg aagtgataat tttatatttc catcaaataa ttcacctatt cttcttatgg
    12181 tggcatctgg ctcttccttc attccagaag aaatccattg aattaccatt tctgtaagtg
    12241 cacattgata gaatgttgct attagtttca tatcatattc agatgcatta ataccctgac
    12301 ttaacttctt aacatacctc tccatcatgt tttctgaaac attataaata taattgacca
    12361 attcttccct ctgcattgaa ttatatacat gataaattac cttcttattt cctaaggcaa
    12421 accttgttgc taatacaaag ctttcctccc aggacatagt gtcattatat tcatctataa
    12481 cattttgaag ctcagtttga aaaagttcag ataaaagctc atacatatct gaataataat
    12541 agtagaaagt atttcgatta attccacaag ataatgctat atcttttaca gtaatcttat
    12601 ttaatggcct ttcatttaat aactctataa atacgctacg aatcattttt ttagtatatt
    12661 gctgagccaa aagaatctcc cccttttgat ttctattaat aaattataac attaacttta
    12721 tattttttca taaaatggtt attattatct ctcataatga caaagtatat tattacttta
    12781 caacacatta tgaaatatat gctttttaga aa
//
LOCUS       DAEVGI010000066        12755 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEVGI010000066 DAEVGI010000000
VERSION     DAEVGI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12755
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.066"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(39..1535)
                     /locus_tag="K8M77_001517"
     CDS             complement(39..1535)
                     /locus_tag="K8M77_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBI7086013.1"
                     /translation="MYNLNDLIDRLIEISKNNNLIEDMDTVYIRNRLLSIFNENSYTP
                     CEEKLTLSFHETLNELINIAIEKKIIEDALYSKDIFSSDIMNIFLPTPSLINKEFYKR
                     YAISPKESTDYFYSLSKSSNYIRTDRIAKNINFKAPSKYGTMDITINLSKPEKDPKQI
                     ALARNSVKSNYPKCLLCIENEGYEGTVTHPDRANHRMIRLSLNDRTWMLQYSPYLYYN
                     EHCIILSEDHVPMKIDISTFKNLLSFVDKFPHYFAGSNADLPIVGGSILSHEHYQGGN
                     HRFPMNDAKKLFDFSIDGFEDVECEAIKWPISTIRLRGENIDSLVLASDLILKKWRDY
                     SDETLDILSYSNSEMHNTITPMVRKEDGKFVVDLSLRNNRTSKEHPLGIFHPHEEVHH
                     IKKENIGLIEVMGLAVLPGRLLKELEKIKEYLRDEISLDNIEEYHRPWALELKKKFDY
                     LKSATDLNDFVNKELSNKFVSVLEHCGVFKLNEEGLEGFKRFTNSLNN"
     gene            complement(1622..2785)
                     /locus_tag="K8M77_001518"
     CDS             complement(1622..2785)
                     /locus_tag="K8M77_001518"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBI7086014.1"
                     /translation="MELNTLKSTFINNFGKEPNSLFFSPGRINLIGEHIDYNGGFVFP
                     CPITLGTFAAATLRDDRICRAYSLNFESLGVIEFSLDDLSYKKEDNWTNYLKGVLKVL
                     IEKGYKIDKGIDLVINGNLPNGAGLSSSASLEMLIVKILDTFFSLNISKVDAALIGKE
                     VENTYIGVNSGIMDQFAISLGEKDKAILLDCNSLYYEYVPLNLGDNSIIIMNTNKRRE
                     LADSKYNERRKECDDSLDTLKKYTNISSLCELTSLEFETYKDKIEDSNKLRRCVHAIS
                     ENERVKDAVKALKENNLELFGQLMNQSHISLRDDYEVTGKELDTLAENAWKQPGVLGA
                     RMTGAGFGGCAIAIVNNNHVDEFIKNVGQAYKDAIGYEASFYVASIGNGPTEL"
     gene            3011..4033
                     /locus_tag="K8M77_001519"
     CDS             3011..4033
                     /locus_tag="K8M77_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBI7086015.1"
                     /translation="MVIESKINDEISKIILKNNKVSVEILRLGAIVEKINVEDRYGNK
                     ENIVLAYKDEESYIENPSSLGSIVGRVAGRIGKGSFKLNGINYELDKNNNGNCLHGGY
                     KGFSKKIWDYSYSEDEEKSTAIFTSFSKDGEGGFPGNLKVQVTYSLEGDKLSIEYKAS
                     SDKDTVVNLTNHSYFNLSGNNKRNILEQKLYIDSDKICELDKNLIPTGEFLSVEKTPF
                     DFREPKKIGEDINKNHLQLEIGSGYDHPWVLNKKGDFDAILVDEESGRIMKIKTDQKA
                     VVCYSMNFPDELPLENGKVAKKHDGICFETQNLPVGYDDCFLDGIILKKDEEYYQKTT
                     FKFECI"
     gene            complement(4289..5317)
                     /gene="mglC"
                     /locus_tag="K8M77_001520"
     CDS             complement(4289..5317)
                     /gene="mglC"
                     /locus_tag="K8M77_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBI7086016.1"
                     /translation="MQTKSKKKFNLNSQWLMNNAIYIVLVVLLIGICIISPDFLSLKN
                     FINILSQSSSRIIIALGVGGILLTEGTDLSAGRTVGLAAVVSASLLQAGDYAYKMYPN
                     LPELPIFIPILIAMAVCGIVGLVNGLVVSKFNVPPFIATLGMMTGIYGLNSIYFDRPP
                     YGAMPIGGLSRSFSNFTLGSIPIYGNIKIPYLVIYAIIVIAVIWTLWNKTKFGKNLYA
                     IGGNREAAVVSGVNVVRTLLLVYMLAGVLYGFAGALEAGRVGSATASTGEMYELDAIA
                     SCVVGGVSTAGGVGTVPGIVTGVLIFQVINYGLAFIGVSPYLQFVIKGLIIVLAVALD
                     MRKYMKKN"
     gene            complement(5289..6836)
                     /gene="mglA"
                     /locus_tag="K8M77_001521"
     CDS             complement(5289..6836)
                     /gene="mglA"
                     /locus_tag="K8M77_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBI7086017.1"
                     /translation="MKDSSNLLEMRNISKEFPGVKALDNVTLKVKKGSVHALMGENGA
                     GKSTLMKCLFGIYHPNSGEIFISGQKVQFKNSKHALDNGVSMVHQELNQVRERNVMDN
                     LWLGRYPKKGLFIDEKKMYDETEKIFKDLDINVNPRDKVSTLSVSQMQMVEIAKAVSY
                     KSKIIVMDEPTSSLTEKEVSHLFKIINKLRKQGISIIYISHKMEEILEISDEVTIMRD
                     GKWIATEKASDLTMDLIIKLMVGRELTDRFPKKNHIPKETILEVNNLSDSKNELKNVS
                     FKLRKGEILGIAGLVGAKRTETLETLFGLREKGSGDIILHGKKVDNSKPFKAMQNGFA
                     LVTEERRQTGIFGKLPIDFNSIIANIDSYKTSTGLLSNGRISKDTQWVINSMKVKTPS
                     QKTLIGSLSGGNQQKIVIGKWLLRKPEILLLDEPTRGIDVGAKFEIYQLINELAKEDK
                     GIIMVSSEMPELLGVCDRILVMSNGMVSGIVNANETTQEEIMHLSAKYLSVTGGVNNA
                     NQIKEKV"
     gene            complement(6910..7974)
                     /gene="mglB"
                     /locus_tag="K8M77_001522"
     CDS             complement(6910..7974)
                     /gene="mglB"
                     /locus_tag="K8M77_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBI7086018.1"
                     /translation="MKKKGLALILISALTMGTLVGCGGGSSSTGSSGDTPKENDSPKI
                     GATIYSFEDNFMSYQRRNIEKLCNGKAELLMNDSQNNQSKQIEQVDTMIAKGVDILAI
                     NLVDPKSAPTVIDKAKADNLPVVFFNKEPDEAVMQSYDKAWYVGTTSEESGIIQGEVM
                     VEGWKANPAWDKNGDGKIQYVMLKGEPGHPDAEARTKYSIETINKAGIETEELAMDTA
                     MWDSTKATEKMDAWIAKNGDNIEMVICNNDGMALGAISSLEKAGYLDGTPEKFIPIYG
                     VDAIPEALDKIKAGKMAGTVLNDAKNQAQALVDSCMNLVNGKEINEGTNWKLDNKKSI
                     RVPYVGITKDNINVAEDSYK"
     gene            8192..9205
                     /locus_tag="K8M77_001523"
     CDS             8192..9205
                     /locus_tag="K8M77_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI7086019.1"
                     /translation="MATIKDIAKGAGVSIATVSRVLNLDETLNVSEETRKKVMEIAEE
                     LNYVPVKERKNKIKNYTIGIVYWYTELQELNDPYFLSIRMAVEKKCNEEKIKFKPIDF
                     QKIINEGTREYRDLDGILAIGIFEEEEISLMEKLSKNIIFVDSSPEEWKYDSVVVDFK
                     YGVNLCLDYLTSLGHKDIGYLGGESIPHNGNEYIRKVNYREKTFKEYMEKMGNYKEKW
                     VLKGRFLPEHGYELMKKVLKEKDLPTAFFIASDPMAIGAYKAIFEEGLRIPDDISILG
                     FDDICTAKFLTPSLTTIKVFTDHMGSIAVETLLERMRTGREIAKKIVLPVKLIERESC
                     KRL"
     gene            complement(9323..10162)
                     /locus_tag="K8M77_001524"
     CDS             complement(9323..10162)
                     /locus_tag="K8M77_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBI7086020.1"
                     /translation="MVSLVLEGGTFRPIFSAGVMDALLDNNIIFPYCIGVSAGITNGF
                     SYFSNQKGRNIEILKKYRNDKRYLSLSNFFKCRSIFGLDFVFDELANKLFPYDFNALS
                     NYKGKVLVGVTNANTGKTEYLDGKVKDEKYMALRATCAIPILFPSIKINGNEYYDGGL
                     LDPIPIKKAIADGNEKHLIVLTRPKSYRKTLEKINKLASKIVGRKYPNLKDTFLSRHI
                     KYNETVKFCEELEKAGKAIIIRPNEDEAIESFEKDVTKLEEAYNYGYNACIKNLEKIK
                     ALF"
     regulatory      10285..10394
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10471..11265
                     /gene="thiD"
                     /locus_tag="K8M77_001525"
     CDS             10471..11265
                     /gene="thiD"
                     /locus_tag="K8M77_001525"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBI7086021.1"
                     /translation="MSKLFNALTIAGSDSSGGAGIQADIKAFSANGVYGMSIITAITA
                     QNTQGVFEIMDVTPEIIEKQIEVIFNDIEVGAIKIGMVSKIESIEAISNALGRVEKLP
                     QIVLDPVMISKSGFKLLSDDAKNTLIEKLIPIATLITPNLPEAEELLGREILTIEDMK
                     KAACDLKKLGAKNVLIKGGHLEGEATDILYDGENFTLFDEERIDTKHTHGTGCTLSSA
                     ITANLSKGMNIVDSVRFGKEYITGAIKNGFELGKGVGPTHHFYKFY"
     gene            11277..12074
                     /gene="thiM"
                     /locus_tag="K8M77_001526"
     CDS             11277..12074
                     /gene="thiM"
                     /locus_tag="K8M77_001526"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBI7086022.1"
                     /translation="MEVLKRENPLIHMITNYVTVNDLAQVTINYGGLPLMATHHDELK
                     VITKMANGLLVNIGTLEPYQMESSMISMKIAKEKGIPSVLDPVGVQASKLRKDFAKKL
                     ILEGEPSLIKGNLAEIKTLIGETSNSIGIDSFEDSLSENTKNKIKEYAKERNLIVVVS
                     GVVDFITNGEESASVKNGTYKMSKITGTGCMLGALLTLALSFYDHKDLRFKEVVKAVS
                     TWGICGELAEERLREKEGLMTFKYNLLDELSIINDEIIKEREKVIYE"
     gene            12067..12690
                     /gene="thiE"
                     /locus_tag="K8M77_001527"
     CDS             12067..12690
                     /gene="thiE"
                     /locus_tag="K8M77_001527"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBI7086023.1"
                     /translation="MSNKDYKKLYLVTDYRIPFNELLEKTKEALIGGVSIVQYRAKNK
                     ETKEMCKEAGALKKLCDEFGALFLVNDRIDVALAVKAHGVHIGQDDMEVSIAREIMPK
                     DAIIGVTVHNKEEALKAIKDGADNLGVGALFSTNSKDDATLMTLETLREIKSVSNIPL
                     YGIGGITPYNLNKDILENLDGVAVISALLNSDNIREKSKEFLNILSK"
BASE COUNT         4581 a         1807 c         1933 g         4434 t
ORIGIN      
        1 aaagtgtaag ctttattaat gatacatctc tattttattt agttattaag tgaatttgta
       61 aatctcttga aaccttctaa gccttcttca tttaatttaa acacaccaca gtgctctaaa
      121 acagatacga atttgtttga taattcctta tttacaaagt catttaaatc tgttgctgat
      181 tttaaataat caaatttctt ctttaattca agggcccatg gtctgtgata ttcttcaata
      241 ttatctaaag aaatttcatc ccttaagtat tcctttattt tttcaagctc ttttaaaagt
      301 cttccaggta aaacagcaag tcccataact tctattagtc ctatgttttc ctttttaatg
      361 tgatgaactt cttcatgagg atggaatatt ccaagaggat gctctttaga agttctgtta
      421 tttctaagag ataaatcaac tacgaatttt ccatcttctt ttcttaccat aggagttatt
      481 gtattatgca tttcactatt agaataagat aatatatcta aagtttcatc tgaataatct
      541 ctccattttt ttagtattaa atcagaagct aatactagag aatctatatt ttctcctctt
      601 aatcttatag tagaaattgg ccatttaata gcttcacatt ccacatcttc aaagccatct
      661 atagagaaat caaatagttt cttagcatca ttcataggga atctatgatt tcctccttga
      721 taatgctcat gggaaagaat agaacctcca actataggta aatcagcatt tgaaccagca
      781 aagtaatgag ggaatttatc cacaaaactc aataaatttt taaatgttga aatatctatt
      841 ttcattggaa catgatcctc tgaaagtatt atgcagtgtt cattataata aagataaggg
      901 gagtattgaa gcatccaagt tctatcatta agacttaatc ttatcattct atgattagct
      961 ctatcaggat gagttactgt tccctcatat ccttcatttt ctatacataa taaacatttt
     1021 ggataattac ttttaacaga atttcttgct aaagctattt gtttagggtc tttttcaggt
     1081 tttgataagt ttattgtaat atccatagta ccatatttag atggtgcctt aaaatttata
     1141 tttttagcta ttctatccgt tcttatataa tttgaacttt tacttaaaga atagaaataa
     1201 tctgtacttt ccttaggtga aatagcatat cttttataaa attccttgtt tattaaagat
     1261 ggtgtaggta aaaatatatt cattatatca ctagaaaata tgtcttttga atataaagca
     1321 tcttctatga ttttcttttc tattgctata tttattagtt catttaatgt ttcatgaaaa
     1381 gaaagcgtaa gcttctcttc acatggagta taactatttt cattaaatat actaagaagt
     1441 ctatttctaa tatatacagt gtccatgtct tctatgagat tattattttt tgatatctct
     1501 attaatctat ctattaaatc atttaaatta tacatgaaaa atccccctta attaaattga
     1561 agagggcatg tatcaaaata ggttttatta ttatattttg atacagccca aaggttttat
     1621 tttaaagttc agtaggacca ttacctatag aagcaacata gaatgatgct tcatatccta
     1681 tagcatcctt ataagcctgt ccaacatttt taataaattc atcaacatga ttattattaa
     1741 ctattgctat ggcacatcca ccaaagccag ccccagtcat acgagcacct aaaactccag
     1801 gttgtttcca agcattttca gctagggtat ctaattcttt accagtaact tcataatcat
     1861 ctctaagaga aatatgagat tgattcataa gttgtccaaa taattcaaga ttattttctt
     1921 ttaaagcttt tacagcatct tttactcttt cattctcaga aatagcatgt acacatctac
     1981 gtaatttatt agaatcttct attttatctt tatatgtttc aaattctaag gaagttagtt
     2041 cgcaaagaga agaaatatta gtatatttct ttaaagtgtc taaagaatca tcacattctt
     2101 ttcttctttc attatattta gaatctgcaa gttcacgtct tttattagtg ttcattataa
     2161 ttattgaatt atctcctaag tttaaaggaa catattcata atataagcta ttacaatcaa
     2221 gtaaaattgc cttatccttt tctcctagag aaatagcgaa ttgatccatt ataccactat
     2281 taacacctat ataagtattt tctacctctt tacctattag tgcagcatct acctttgaaa
     2341 tgttaagaga aaagaaagta tctaaaattt ttactattaa catttctaaa gatgctgaag
     2401 aggaaagacc agctccgttt ggaagatttc cattgataac taagtcaata cctttatcta
     2461 ttttatatcc tttttctata agtactttta gtactccttt aagatagttt gtccaattat
     2521 cctctttttt ataagataaa tcatctaaag aaaactctat tactccaaga gattcaaagt
     2581 taagagagta agctctacaa attctatcat ctcttaaggt tgccgctgca aaagttccaa
     2641 gagttattgg gcaaggaaat acgaatcccc cattgtagtc aatatgttca cctataagat
     2701 ttattcttcc tggtgagaag aataatgaat taggttcttt accaaaatta ttaataaaag
     2761 ttgatttaag tgtatttaat tccatgatat acctccaaat agaaaatctg taacttaatt
     2821 atacgattac aaaaaataaa aatcaattgt tttactaaaa attttactaa ttttttgtta
     2881 agttattttt aaatatagaa tttttgataa aattcttaaa aatagcatgt ttaatagagt
     2941 aaatattatt gaaaaactta tattaagttg ttaaaatcaa actatatata tgaaagggag
     3001 agtgaataat ttggttatag aaagtaaaat taatgatgaa ataagtaaaa taatattaaa
     3061 aaataataaa gtatctgtgg aaatattaag attaggtgct attgtagaaa aaataaatgt
     3121 agaggataga tatggaaata aagaaaatat agtgcttgct tataaggatg aggaaagcta
     3181 tattgaaaat ccttcaagtt taggttctat agtaggaaga gtagcaggta gaataggaaa
     3241 gggttccttt aaattaaatg gtattaatta tgaattagat aaaaataata atggaaactg
     3301 ccttcatgga ggatataaag gttttagtaa aaaaatctgg gattattcat atagtgaaga
     3361 tgaagaaaaa tctacagcaa tatttacttc ttttagtaag gatggagaag ggggattccc
     3421 aggaaattta aaggttcagg ttacttattc tctagaagga gacaagcttt ccatagaata
     3481 taaagcttct tcagataaag atacagtagt aaatttaact aatcattcat attttaattt
     3541 atcaggaaat aataaaagaa atatattaga acaaaagctt tatatagatt cagataaaat
     3601 atgtgagtta gataaaaatt taattccaac aggagagttt ttaagtgtag aaaaaacacc
     3661 ttttgatttt agggagccaa aaaagatagg tgaggatata aataaaaacc acttgcaatt
     3721 agaaattgga tcaggatatg atcatccatg ggttttaaat aagaagggtg attttgatgc
     3781 aattttagta gatgaagaat caggaagaat tatgaagatt aagacagatc aaaaggcagt
     3841 agtatgttat tcaatgaatt ttccagatga attgccttta gaaaacggca aagtagccaa
     3901 aaagcatgat ggaatatgtt ttgaaacaca aaatttacca gtaggatatg atgattgctt
     3961 cttagatgga ataatactta aaaaggatga agagtattat caaaaaacaa cttttaaatt
     4021 tgagtgtata taaaaaaatg gagtgtatca aaaacttttt ataaaactta caaacagtat
     4081 gaagagaata tctagatgag ctttagaatt taaagtatta cggagaagat attacgttaa
     4141 aaagctgaaa ctgttcgagg cgaagcaagt tttgaagctt tagtaatatc ttcggagtaa
     4201 tacttataaa ttcttagcga aatctagtaa ttctcagaat atgtttgtaa gttttaattt
     4261 gttatgtttt gatacatccc catttttatt agttcttttt catgtatttt ctcatatcaa
     4321 gagctacagc tagaactata attaaacctt ttataacgaa ttgtaaataa gggctaacac
     4381 ctatgaaagc taggccatag tttataactt ggaatattaa aacaccagtt actattccag
     4441 gaacagtacc aacaccacca gcagtggaaa ctccaccaac aacacaggaa gctatggcat
     4501 ctaattcata catttcacca gtactagcag tagcactacc aacacgacca gcttctaggg
     4561 cacctgcaaa accataaaga actccagcta acatataaac taataaaagt gttctaacaa
     4621 catttacacc tgaaaccaca gcagcttctc tgttaccacc tatagcataa aggtttttac
     4681 caaatttagt tttattccat aaagtccaaa ttacagctat tacaataatt gcatatataa
     4741 ctaaataagg tatttttata tttccatata tggggattga tccaagtgta aaattgctaa
     4801 aggatcgact aagaccacct attggcatag ctccatatgg aggtctatca aagtatattg
     4861 agttaagtcc atatattcct gtcatcattc ctagagtagc tataaatgga ggaacattaa
     4921 atttagaaac aactagtcca tttacaagac caacgattcc acaaacagcc attgctatta
     4981 aaataggaat gaatataggc aattcaggta aattaggata cattttatat gcataatcac
     5041 cggcttgaag taatgaagct gatactacgg cggcaagtcc aactgttcta ccagcggaaa
     5101 ggtcagtacc ttcagttaaa agtattcctc caaccccaag ggctattatt atacgagaag
     5161 aggattggct taatatatta ataaagtttt ttaatgataa aaaatctggt gaaataatac
     5221 aaattcctat taataaaact accaaaacta tataaatagc attattcatt aaccactggc
     5281 tatttaaatt aaactttttc tttgatttgg tttgcattgt taactcctcc tgttactgat
     5341 agatattttg cagatagatg cataatttcc tcttgggtag tctcattagc attaactatg
     5401 ccagaaacca taccattact catgactagt attctgtcac atacacctaa aagttcaggc
     5461 atttcagaag aaaccataat tattccttta tcttctttag caagttcatt tataagttgg
     5521 tatatttcga atttagcacc aacatctata cctctagtag gctcatctag aagtagtatt
     5581 tcaggttttc taagaagcca ttttccaatt actatctttt gttgattacc accagatagg
     5641 ctaccgatta gggttttttg acttggggtt ttcactttca ttgaatttat aacccattga
     5701 gtatctttag atattcttcc atttgataaa agaccggttg atgttttata actatctata
     5761 ttagctatta tagaattaaa atctataggt aattttccaa agattccagt ttggcgtctt
     5821 tcttcagtaa caagggcaaa accattttgc atagccttaa aaggcttact gttatcaact
     5881 tttttgccat gtaaaataat atctccagat cccttttctc taaggccaaa taaggtttct
     5941 aaggtttcag ttcttttagc accaacaaga cctgcaattc ctaaaatttc tccctttcta
     6001 agtttaaatg aaacattctt taattcattt tttgaatcac taagattatt tacttctaaa
     6061 atagtttctt taggaatatg attcttttta gggaatctat cagtaagttc acgtccaacc
     6121 ataagtttta ttattaaatc catagtaaga tctgaagctt tttcagtagc aatccattta
     6181 ccatctctca ttatggtaac ttcatcggag atctctaaga tttcttccat cttatgagag
     6241 atataaatta tacttattcc ttgttttcta agtttattta ttattttaaa taggtgactt
     6301 acttcttttt ctgttaaaga ggaagtaggc tcgtccatta ctattatttt agatttatac
     6361 gaaacagcct ttgctatttc aaccatttgc atttgagaaa cagatagggt agaaacctta
     6421 tcacgaggat taacattaat atctagatcc ttaaatattt tttctgtttc atcatacatt
     6481 ttcttttcat ctataaaaag ccctttttta ggatatctac caagccaaag attatccata
     6541 acatttcttt ctctaacttg atttagttct tggtgaacca tagatactcc attatctaag
     6601 gcatgttttg aatttttaaa ttgtaccttt tggccagaaa taaaaatttc tcctgaatta
     6661 gggtgatata taccaaatag acatttcatt aatgttgatt taccagcacc attttctccc
     6721 attaatgcat gtacagaacc tttttttact tttaaggtta cattatctaa ggctttaact
     6781 cctgggaatt ctttagagat atttcgcatt tctaacaggt ttgaagagtc tttcatcttt
     6841 ttttacctcc tgttttatta agggcatata tcaaaattaa ttttgatata gccccttatt
     6901 ttttataaat tatttatatg aatcctcagc aacgtttata ttgtctttag ttattcctac
     6961 atatggaaca cggattgatt ttttattatc aagtttccaa ttagttcctt catttatctc
     7021 ttttccattt actaaattca tacaagaatc aactagagct tgagcttgat tcttagcatc
     7081 gtttaatact gtaccagcca ttttaccagc tttgatttta tctaaagctt caggaatagc
     7141 atctacacca tatattggaa taaacttttc aggagttcca tctaaatatc ctgctttttc
     7201 aagagaagaa atagcaccta aagccattcc gtcattatta cagattacca tttctatatt
     7261 gtcaccattt ttagcaatcc aagcatccat cttttcagta gctttagttg agtcccacat
     7321 agctgtatcc attgctaact cttcagtttc tataccagct ttatttattg tttcaataga
     7381 atattttgta cgagcttctg catcaggatg tccaggctct cctttaagca ttacatattg
     7441 tatttttcca tcaccatttt tgtcccaagc aggattagct ttccaacctt ctaccattac
     7501 ttccccttgg attattccag attcttcaga ggttgtacca acataccaag ctttatcata
     7561 gctttgcata acagcttcgt ctggttcctt attaaagaat accactggta agttgtctgc
     7621 cttagcttta tctattacag taggagcaga ttttggatca actaagttta ttgcaagaat
     7681 atcaacacct ttagctatca tagtgtctac ttgttctatt tgcttagatt gattattttg
     7741 agagtcattc attaataact cggcttttcc attacaaagt ttttctatat ttctacgttg
     7801 gtatgacatg aagttatctt caaaactata aattgtagct cctatcttag gtgaatcgtt
     7861 ttcttttggc gtatcaccag atgatccagt agagcttgaa cctccaccac aaccaactaa
     7921 agtacccata gttaaagctg aaattaaaat tagtgctaaa cctttctttt tcataacaag
     7981 tcctccctat tttaccttaa aactttatag taattttaag taaattataa cataaaatgg
     8041 aaataaaaca agagaaattt gttaaagaat aataaagaaa gtattatttt atatttgttt
     8101 agttaaattt tatatattat acaaagaaaa ggttagaaaa ttttagtaaa aaaatatata
     8161 atataaaata agaaaagtaa ggagtgatta tgtggctact attaaagata ttgcaaaggg
     8221 tgctggagtt tcaatagcta ctgtatctag agttttaaat ttagatgaaa cgcttaatgt
     8281 atctgaagaa acaagaaaaa aagtaatgga aatagctgaa gaattaaatt atgtaccagt
     8341 aaaagaaaga aaaaataaaa taaaaaatta caccatagga atagtttatt ggtacacaga
     8401 actacaagaa ttaaatgatc catattttct ttcaataaga atggctgttg agaaaaagtg
     8461 taatgaagaa aagattaaat tcaaaccaat agattttcaa aagataatta atgaaggaac
     8521 aagagaatat agggatttag atggaatatt ggctattgga atatttgaag aagaagagat
     8581 aagcttaatg gaaaagttat ctaaaaacat aatttttgta gattcttctc cagaagagtg
     8641 gaaatacgat tcagtggtag tagattttaa gtatggagta aatctttgtt tagattattt
     8701 aacatcctta ggccataaag atattggata tttaggggga gaatcaattc ctcataatgg
     8761 taatgagtat ataaggaagg taaattacag agagaaaacc tttaaagaat atatggagaa
     8821 aatgggtaac tataaagaaa agtgggttct aaagggaaga tttttaccag agcatggtta
     8881 tgaacttatg aagaaggtat taaaagagaa ggatcttcct acagcatttt ttatagctag
     8941 tgatcctatg gccataggag cctataaagc aatatttgaa gaaggattaa gaataccaga
     9001 tgatataagt atattaggtt ttgatgatat atgtactgct aaatttttaa caccttcctt
     9061 gacaacaata aaagttttta cagatcatat gggaagtatt gccgttgaga cacttttaga
     9121 gagaatgaga acaggaagag aaatagctaa gaaaatagtt ttacctgtaa aactaataga
     9181 aagagaaagt tgtaaaagac tttaaagata aatttacttt tgtttaacta aaagagacta
     9241 catgaaattt tttatgtagt ctcttttagt tgtggtaaaa ataattttaa atatacttta
     9301 agatttaaaa tagagattaa tattaaaata aagcctttat tttttctaag ttttttatgc
     9361 aggcattata tccatagtta taagcctctt ctaatttagt tacatctttt tcaaaacttt
     9421 caatagcttc atcttcattt ggtcttatta taatggcctt accagccttt tctaattctt
     9481 cacaaaattt tactgtttca ttatatttta tatgtcttga taaaaatgta tcttttaaat
     9541 tagggtattt acgtcccact attttagatg ctaacttatt aattttttct aaggtttttc
     9601 tataactttt aggtctagta agaactatta agtgtttttc atttccatct gctatagctt
     9661 tttttattgg aataggatct aatagtccac catcataata ttcatttcca tttattttta
     9721 ttgatgggaa aagtatagga atagcgcaag ttgctcttaa agccatatat ttttcatctt
     9781 taacttttcc atctaagtat tctgtttttc ctgtattagc atttgtaaca cctactaaaa
     9841 cttttccctt ataatttgaa agtgcattaa aatcataagg gaagagttta tttgctaatt
     9901 catcaaatac aaaatctaaa ccaaatatgc ttctacactt gaagaagtta gaaagactta
     9961 aatatctctt atcatttcta tattttttta atatctcaat atttcttcct ttttgattag
    10021 aaaaatatga aaaaccatta gttattcctg ctgaaactcc aatacaataa ggaaatatta
    10081 tattattgtc aagaagagca tccataactc ctgcactaaa aataggacga aaagttcctc
    10141 cttctaaaac taagctaacc ataaaatatc ctcctaagct tcataatata tttaagatta
    10201 taatccttaa attaaaaatt ttcaaaaaag gtatgttgaa aaagaaggat gattatgata
    10261 tcatttggtt tagaaaatag tataaaaaat agctaggggt gccagtagtg tctggctgag
    10321 attagaaatt aaatttcttg accctttaac ctgatctggt taataccagc gtagggaagc
    10381 ccaagagaca catatattta aattgatttt aaagcatata tagccttagg gctatatatg
    10441 ctttttcttt ttttagaagg agtgaaaaat atgagtaaat tatttaatgc attaacaatt
    10501 gcagggtcag atagttcagg gggagcagga atacaggctg atataaaagc tttttcagct
    10561 aatggagttt acggaatgag tataataact gccataacag ctcagaatac tcaaggagtt
    10621 tttgaaataa tggatgttac tcctgaaatc atagaaaaac agatagaggt tatttttaat
    10681 gatatagaag taggtgccat aaaaataggt atggtatcta aaattgaatc aatagaagcc
    10741 ataagtaatg ctttaggaag agtagaaaaa ttacctcaaa tagtattaga tcctgttatg
    10801 atttcaaaaa gtggatttaa gcttttatca gacgatgcaa aaaatacttt gatagagaaa
    10861 ttaataccaa tagctacttt aataactcca aatttaccag aggcagaaga attattaggt
    10921 agagaaatac ttacaataga agatatgaaa aaggcagcct gtgatcttaa aaaattagga
    10981 gcaaaaaatg ttttaattaa gggtggacat ttagaaggag aggctacaga tattttatat
    11041 gatggtgaaa actttactct ttttgatgaa gagagaatag atacaaagca tacccatgga
    11101 acaggatgta ccttatcatc agctattaca gctaatttat caaagggaat gaatatagtt
    11161 gattctgtga gatttggaaa agaatatatt acaggtgcta taaaaaatgg gtttgaactt
    11221 ggaaaaggtg ttggaccaac acatcatttc tataaatttt actaggagga ataaaaatgg
    11281 aagttttaaa aagagaaaac cctttaatac atatgataac taattatgtt acagttaatg
    11341 atctagcaca agtaactatt aattatggcg gcttacctct tatggctaca catcatgatg
    11401 aattaaaagt gataacaaaa atggctaatg gattattagt taatataggc actttagaac
    11461 cttaccaaat ggagtcatca atgatttcta tgaaaatagc aaaggaaaaa ggaattccct
    11521 ctgttttaga tccagtaggt gtacaagcat caaaacttag aaaagatttt gctaaaaaat
    11581 taatactaga gggagagcct tcacttataa aggggaattt agctgaaata aaaaccctta
    11641 taggtgaaac ttcaaattca attgggatag attcctttga ggattcttta agtgaaaata
    11701 caaaaaataa gataaaggaa tatgcaaagg aaagaaatct tatagtagtt gtttctggag
    11761 tggttgattt tataactaat ggagaagaaa gtgcaagtgt taaaaatgga acttataaaa
    11821 tgagtaaaat tacaggcact ggatgtatgc taggagcttt attaacacta gctttaagtt
    11881 tttatgacca taaagattta agatttaaag aagtggttaa agcagtatct acatggggaa
    11941 tatgtggaga attagctgag gaaagattaa gagaaaaaga aggattaatg acatttaagt
    12001 ataatctttt agatgaactt tcaattatta atgatgaaat aataaaggag agggagaaag
    12061 ttatttatga gtaataaaga ttacaaaaaa ttatatttag taacagacta tagaattcct
    12121 tttaatgagc ttttagaaaa aacaaaggaa gctttaattg gaggggtatc cattgttcaa
    12181 tatagggcaa agaataaaga aactaaagaa atgtgtaaag aagctggagc tttaaaaaag
    12241 ctttgtgatg agtttggagc attattctta gtaaatgata gaattgatgt agccttagca
    12301 gtaaaagctc atggagttca tatagggcaa gatgatatgg aagtttccat agcaagagaa
    12361 ataatgccaa aggatgcaat tataggtgtt actgttcata ataaagagga agctttaaaa
    12421 gctataaaag atggagcaga taacttaggt gttggagctt tattttcaac taatagcaaa
    12481 gatgatgcaa cattaatgac tttagaaacc ttaagagaga taaaatcagt ctctaatatt
    12541 ccactttatg gaataggggg tataactcct tataacttga ataaggatat tttagaaaat
    12601 ctagatgggg tagctgtcat atctgctttg ttaaatagtg ataatataag agaaaaaagc
    12661 aaggaatttt taaatattct tagtaaataa gaaattttga atattaaact taaaatatga
    12721 aataatcaaa agaggttctt aatttataga atctc
//
LOCUS       DAEVGI010000067        12579 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEVGI010000067 DAEVGI010000000
VERSION     DAEVGI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12579
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.067"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            112..660
                     /locus_tag="K8M77_001528"
     CDS             112..660
                     /locus_tag="K8M77_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide protein"
                     /protein_id="HBI7086024.1"
                     /translation="MKNLNVRRIFLLLLVIGITGSLGSVSYFSDKENVNGDLRLTLGT
                     LSSEATKTVKIEKMDIEVPVSDTFIITNNGTLDQRITLNFKNSSIGNDGLEKIKYSLS
                     FESSENRNIQGYGNGEETLLSLFNKNIQLLDAEGNEIVLNDGEVLTATLTLNMQRDMP
                     ENYSNAEFKFDLNIGYTQENSK"
     gene            677..1195
                     /locus_tag="K8M77_001529"
     CDS             677..1195
                     /locus_tag="K8M77_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086025.1"
                     /translation="MISKNKIIALILGVSLASSATYESYAYFSGEYSVKNTVKIVELD
                     PKESPFEISKISTRENSLVLELSKKVFYSGEDINSDIVGSETFLDNVEISIEVIDQPN
                     NEVKEESSIENSNNEVEDIETPKSGDENTTPVEGKNLSKEENLEQKNINGNELNKSLG
                     KGEISNNLTNIN"
     gene            1407..3662
                     /gene="pcrA"
                     /locus_tag="K8M77_001530"
     CDS             1407..3662
                     /gene="pcrA"
                     /locus_tag="K8M77_001530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBI7086026.1"
                     /translation="MDLKSLLNKEQYEAATTIDGQVLILAGAGSGKTRVLTHRIAYMI
                     ENDIKPYNILAITFTNKAAGEMRERVKSLVGEVANNMWISTFHSSCVRILRREIDKLG
                     YSKDFTIYDSSDQKTLIKLVMKELNINEKEITDLEILGTIGKAKDNIQSAQSFKKENE
                     SNFRKNKIADAYLLYQKKLKENNALDFDDLIVKTVELFRKHNDVLEFYQNKFKYIMID
                     EYQDTNKAQYEFAKLLAAKHKNICVVGDDDQCIYAWRGADIRNILDFEKDYPEAKVVK
                     LEQNYRSKGNILEAANKVIKNNAERKFKILRTEQVEGEKISIHRAYSDKMEAAFVVSE
                     IEKLKSDKGYKYKDFAVLYRTNAQSRNFEEALRRAGIPYRIFGGLKFYDRKEIKDVLA
                     YLKIIVNHKDSISLRRIINVPKRSIGDTTVEKLQNHASEIDDTLYNVLLDVDYVPGLT
                     ARSINPIKKFTDMMEEIMVMSEQLSVSQLIEYVLEKTGYLKSLKDSKLIEDQSRIENL
                     EELVSDAVEFEKSNEEDKSLSAYLEKVALVQDMDDLEAEDNYVMLMTVHSSKGLEFPT
                     VFLVGMENGIFPNAACFEHDNQMEEARRLCYVGITRAEEKLYMTSAETRMIFGKTVAY
                     GQSDFISEIPANIKDYVNINGKSSSNSFKNSSQTYGETRKTYNPHSIRGGMSLSKANE
                     SLNKTLSGKSSGNAGIDELTMGRKVKHPKFGVGTLITKSKVGDDYKLTIAFDSQGVKH
                     LMFSFAPLELL"
     gene            3700..5688
                     /gene="ligA"
                     /locus_tag="K8M77_001531"
     CDS             3700..5688
                     /gene="ligA"
                     /locus_tag="K8M77_001531"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBI7086027.1"
                     /translation="MDKKKLIEELVEELNKYAYEYYVLGNSSVTDKDYDKKYYELVDL
                     EKETGYKLPYSPTQRVGDVILPEFKKYTHKARLWSLDKAQTLEEIREWHNRNIKFLEE
                     YNRTSDEELPPLKYILTKKFDGLTINLSYDENGVLITGATRGTGAIGEDVTAQVKTIK
                     SIPLKIDCHDFLEIHGEAIMTTEAFEKYNSEADTPLKNLRNGAAGALRNLNVAETAKR
                     NLSAFFYDVGYKEGAPFKTYMEMLNFIKTKGFPMDDYIRECKTLDEIQKEIDYIRDIR
                     FDLNYDIDGLVIAIDDIRTRELLGYTVKFPKWAIAYKFEAQEATTKLLDVEWNVGRSG
                     RVSPTAILEPVELAGVTVKRATLNNMDDIARKGVRLGAEVFVRRSNDVIPEIMGVVPE
                     SLEGTKEIEEPKVCPACGAHLVHEGVHIYCENTLGCKPQMVKTIVHFAGREAMNIAGF
                     SERTAEQLFEKLDIRDISDLYKLEYEKLLDLDKFGPKKAQNLLDAIEKSKDCTLEAFL
                     YSLGIPNVGVKTAKDLVKRFESLENLEKATFEELVSVQDVGDIVARSIIEFFKEERTL
                     KVINELLSLGVNPHYEKKEVLESPFMGKTVVVTGTLENYSRTSIKEKLESLGAKVSGS
                     VSKKTDFVIAGEAAGSKYDKAKSLGITILSEEEFENMI"
     gene            complement(5723..6046)
                     /locus_tag="K8M77_001532"
     CDS             complement(5723..6046)
                     /locus_tag="K8M77_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086028.1"
                     /translation="MTLAVQVVAILFMLIMIFLGIWSFVIANKAYRQIKYKNYLLEKI
                     AHNISLIRNKEKIFDNLNTMADSVSYSEIKENLLDKDIKADTNLEEDTNISEEIKENS
                     NIVEK"
     gene            6170..6634
                     /locus_tag="K8M77_001533"
     CDS             6170..6634
                     /locus_tag="K8M77_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBI7086029.1"
                     /translation="MLKSFYVLMNSKNIEKSLMKFRKISIKKVSQIIIKECIKEKLNY
                     EIIEKNNNEFIVEISSSRKKTLIVFHKALAVTIYDYYKFIRLIQDRKIDKGVYITTGV
                     FQQDVYNMAETDRFINRIKLLNGKDFVVKQRWIKRKKHHHISYDKLSFKSLF"
     gene            6844..8334
                     /locus_tag="K8M77_001534"
     CDS             6844..8334
                     /locus_tag="K8M77_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBI7086030.1"
                     /translation="MKKVILFICLIILLVVGVGRSYVDVTPVSNMNFKYNEGLIYSLE
                     SLPDTIRPSESLNKGEKDLVSNIFSGLVEVNEKGEPIPDLAMGWKVSDDGLEYTFKIN
                     EKNKWSNGTEVTAKDFQIFFRDLLSPDNEDYTSNELYSIYGVKDYREGKIDFSEVAIS
                     SPDDYTLVLRMNNKDDDLLKNLAKPIYRLRDLNEPLDNYKSDFNEISYTGPYVIYDVT
                     KDGFIRLKDNPYNSSELEIKDIAFKEKESDEIELAAFNLEKVDILKNPPIIYSEDIGL
                     YKDLSKYNSDILKLMIVNSDKAEIAQGISNIMQVLISDSNILKNNLGKPYIKVINNKD
                     KIHSIKKDGDKGDYKINESEKNNLKESGKNTLKKVFNNNEVVKIVVGNNEEDKALANE
                     LKKFLDKELAIKSIVKIHDEGIEKVIKSGEFNIAFGEFNLNEKNSESLKQEDINKEEE
                     KETATSEKSNLSLVSLYFKNDVWCKSPKVNYLFIDSNGNLILKYSS"
     gene            complement(8602..10305)
                     /locus_tag="K8M77_001535"
     CDS             complement(8602..10305)
                     /locus_tag="K8M77_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086031.1"
                     /translation="MKYIGSFFKANSLSKEEMASQLLFLSKESVNHIVLESRCGISTN
                     YKNLKENFKKDEISFFKNNTPLICIYKKAKPNIYSSKYSKTWDDTTFKKDIPVSSNAL
                     MTLSLLRLCSYYEKFKNINSALYKRAAFYKNLSKLQLEFYYTYLRNPEGFFVNKKNDE
                     SSSKSGLNISEKEDEISYSDQALMMLAYYMYSKVAPEDSDSKTYEDFALQILNMFVNF
                     KEELYLLSLDECCKIDYCLNNMLLYSDNEDCRLLVLDLSDFILNKYYESNSTFSNLET
                     TSLLALNMYLSYKNTNILIFKDAYLDLIELFCNMFNDEKGIILKGTDKKEYKYSNMEI
                     CLYLTNLLMSFDLDEDSSDKRENIISKVYKNYIVNSGIVTSFPDAPNLDSHERYKKFS
                     LKPDDLIDESMFRMTNANSPELTGLAPIFTKNLKYSTKKSIFTSDKTTFDSTKNMFIF
                     FVFINSFIDKYIDFITDTPKEKKSAMLVEEIDSLKNVQKINKLPKLNNFNSKTTIVPP
                     DAGESDYNSITNENQVDEISPPPTNPNLTREISEYSDSSIDKKEDLDDKMKNILDFTD
                     L"
     gene            10527..11273
                     /gene="rph"
                     /locus_tag="K8M77_001536"
     CDS             10527..11273
                     /gene="rph"
                     /locus_tag="K8M77_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBI7086032.1"
                     /translation="MRSSGRKKEQIRPVKITRNFTKYAEGSVLIEVGDTKVLCTASIE
                     EKVPPFLKGSGEGWITAEYNMIPRSTQSRKQRDINKLKIDGRTMEIQRLIGRALRSAV
                     DMKALGEKTIWIDCDVLQADGGTRTTSITGSFVALVDAVNKLHQKKPFNVYPIRHFVS
                     AVSIGIVGEEKVLDLCYEEDHVAKVDMNVVMTEEGEFIEIQGTGEIGPFSRKELDELL
                     NLAEKGAKQMIQAQKNALKTDSLWIGTGRE"
     gene            11275..11889
                     /locus_tag="K8M77_001537"
     CDS             11275..11889
                     /locus_tag="K8M77_001537"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBI7086033.1"
                     /translation="MKKFILASNNAHKVKEIKEILKDFNLNILSLNEAGIDIDVEEDG
                     KTFEENSFKKANEIRKYLLSRGESDFIVMADDSGLEVDYLNGAPGIYSARYAGEHGND
                     SKNNEKLLEELKNVKDDERKANFICVIVAVTDKGEKIVAEGKSYGVILEALSGNEGFG
                     YDPLFFVPEYKKTFAEMTSDEKNAISHRGRALEKLKDNLKGILK"
     gene            11901..12371
                     /locus_tag="K8M77_001538"
     CDS             11901..12371
                     /locus_tag="K8M77_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI7086034.1"
                     /translation="MKVAIVSDTHGVTHVIEYIKTLLNDSDVLIHLGDNINDVKLLSE
                     SFKGKVYSVVGNCDYERNGQVEQVIEIEGKKFLITHGHKYTVKYGLDKIYYRGLELGV
                     DGVIFGHTHRKVALKEGNMWIINPGSPSIPKDGEASIAFMNVDSENIEVRFFSI"
BASE COUNT         5104 a         1224 c         2196 g         4055 t
ORIGIN      
        1 tatgttaata atagattata tgttagtaag aaatagataa taaagttaaa aaggtaaaaa
       61 gttttaggaa tattaatatt aaatttaaat caaaaagtaa ggagctttaa tatgaaaaac
      121 cttaatgtga gaagaatatt cttgttgtta ttagttatag gtataacagg tagtttagga
      181 agtgtatctt atttttcaga taaagaaaac gtaaatggtg atttaaggtt aactttagga
      241 accttaagtt ctgaggctac aaaaacagtt aaaatagaga aaatggacat agaagttcct
      301 gttagtgata cttttataat aactaataat ggaactttag atcaaaggat aacattaaat
      361 tttaaaaatt catcaatagg aaatgatggg ctagagaaaa taaagtattc cttaagtttt
      421 gaaagtagtg aaaatagaaa tatacaagga tatggaaatg gagaagagac attactttca
      481 ctatttaata aaaatattca attattagat gcagagggta atgaaatagt tttaaatgat
      541 ggtgaagttt taactgctac attaacatta aatatgcaaa gagatatgcc agagaattat
      601 tctaatgcag aatttaaatt cgatttaaat atagggtata ctcaggaaaa tagtaagtag
      661 tagaggagga gtttaagtga ttagtaagaa taaaattata gcattaattt taggagtttc
      721 acttgcttca agtgctactt atgaaagtta tgcttatttt agtggagagt attcagtaaa
      781 aaatacggtt aaaatagttg agttagatcc taaggaaagt ccttttgaaa tttcaaagat
      841 aagtactaga gaaaactctc ttgtattaga gctttctaaa aaagtcttct acagtggaga
      901 ggatataaat agtgatattg ttggaagtga aactttttta gataatgttg aaatttcaat
      961 agaggttata gatcaaccta ataatgaagt taaagaagaa tcttcaatag aaaattctaa
     1021 taatgaagtt gaagatattg agactccaaa gagtggagat gaaaatacta cacctgtaga
     1081 aggaaagaat ttatctaagg aagaaaattt agagcaaaaa aatattaatg gaaatgagct
     1141 aaataaatct ttaggaaagg gagaaatttc aaataatctt actaatatta attaaggaaa
     1201 aatatatgaa ataaattaaa gaggtaaatt taaaagatta tatttaaagt aagagtatta
     1261 tatttgaaat aaatggtcac attttataat gtggccattt attttttatt aatcatgtaa
     1321 aagaataata tgtttgatag aatataatat atagatttat atggtataat aaagtttagt
     1381 tttattgtag gaagaggagt gatttaatgg atttaaaaag cttgcttaat aaagaacaat
     1441 atgaagcagc aaccacgatt gatggtcaag tacttatatt agcaggagca ggttctggta
     1501 aaactagagt acttacacac agaatagcat acatgataga aaatgatata aaaccttata
     1561 atatattagc aataacattt acaaataaag cggcagggga aatgagagag agagtaaaat
     1621 ctctagttgg tgaagtggca aacaacatgt ggatatcaac atttcattca tcttgcgtga
     1681 gaattttaag aagagaaata gataaattag gatatagtaa agactttaca atatatgatt
     1741 catcagatca gaaaacatta ataaaacttg taatgaaaga gcttaatatt aatgaaaaag
     1801 agataactga tttagagatt ttaggtacta taggaaaagc aaaggataat atccaaagtg
     1861 cacaaagttt taagaaagaa aatgaaagta attttagaaa aaataaaata gcagatgcat
     1921 atcttttata ccaaaagaaa ttaaaagaaa ataatgccct agattttgat gatttaatag
     1981 taaaaactgt tgagttattt agaaagcata atgatgtatt agagttttat caaaataagt
     2041 ttaaatacat aatgatagat gagtatcaag atacaaataa agctcaatat gaatttgcta
     2101 agcttttagc tgcaaaacac aaaaatatat gcgtagttgg tgatgatgac caatgtattt
     2161 atgcttggag aggggcagat ataagaaata tattagattt tgaaaaggat tatccagagg
     2221 ctaaagttgt taagcttgag caaaactata gatcaaaagg aaatatttta gaggctgcta
     2281 ataaagttat aaaaaataat gcagagagaa aatttaaaat tttaagaact gagcaagtag
     2341 agggagaaaa aataagtatt catagagctt actcagataa aatggaggca gcttttgttg
     2401 tttcagagat agaaaaatta aaaagtgata aaggatataa gtataaggat tttgctgttc
     2461 tttacagaac aaatgcacag tctcgtaatt ttgaggaagc cttaagaaga gcaggaattc
     2521 catatagaat ttttggagga ctaaaattct atgatagaaa agagataaaa gatgttcttg
     2581 catacttaaa aataatagta aatcataaag attctataag tttaagaaga ataataaatg
     2641 ttcctaaaag aagcattgga gatactactg tagaaaaact tcaaaatcat gcgtccgaaa
     2701 tagatgatac attatacaat gttttattag atgtagacta tgtgccagga ttaacagcta
     2761 gaagcataaa tcctattaag aagtttactg atatgatgga agaaataatg gttatgtcag
     2821 agcaattaag tgtatctcaa ttaattgaat atgttttaga aaaaacaggt tatttaaaat
     2881 cacttaaaga ttcaaagctt atagaggatc aaagtagaat agaaaactta gaagaattag
     2941 tttctgatgc tgttgaattt gaaaagagta atgaagaaga taaatcatta tcagcttact
     3001 tagagaaggt tgcccttgtt caagatatgg atgatttaga agcagaagat aattatgtaa
     3061 tgctaatgac agttcacagc tcaaagggac tagaattccc aacagttttc ttagttggta
     3121 tggaaaatgg aatattccca aatgcagctt gctttgaaca tgataatcaa atggaagagg
     3181 ctagaagact ttgctatgtt ggaataacta gagctgagga aaaattatat atgacatcag
     3241 ccgaaacaag aatgatattt ggtaaaacag tggcttatgg acaatcagat tttatatcag
     3301 aaattccagc taacataaaa gactatgtaa atatcaatgg taaaagttca agtaattctt
     3361 tcaaaaatag ttcacaaact tatggagaga ctagaaaaac ttataatcct cacagtataa
     3421 gaggagggat gagtctttct aaagcaaatg aatccctaaa taagacttta tcaggaaaat
     3481 cttcaggtaa tgcaggaata gatgaattga ctatgggaag aaaagttaag catcctaagt
     3541 ttggtgtagg aacattaata acaaagagta aagttggaga tgactataag cttacaattg
     3601 cctttgatag tcaaggagtt aaacatttaa tgtttagttt tgcaccacta gagcttcttt
     3661 aatatagaaa tttactttaa taaggagtag atgagtaaga tggataagaa aaaattaata
     3721 gaagagttag ttgaagagtt aaataaatat gcttatgaat attatgtttt aggaaattct
     3781 tcagtgacag ataaagatta tgataaaaaa tactatgaat tagtagatct agaaaaggaa
     3841 acaggatata aattacccta ttcacctact caaagagttg gtgatgtaat tcttcctgag
     3901 tttaaaaaat acactcataa agctagactt tggagtttag acaaagctca aactttagag
     3961 gaaattagag agtggcataa tagaaatatt aaattccttg aagaatataa tagaacttca
     4021 gatgaagaat taccaccatt aaaatatatt ttaactaaaa agtttgatgg attaacaatt
     4081 aatctttcct atgatgaaaa tggagtatta attactggag caactagagg aactggggct
     4141 attggtgaag atgtgacagc tcaggttaaa actataaaat caattccatt aaaaattgat
     4201 tgtcatgatt ttctagaaat tcatggagag gctattatga ctacagaagc ctttgaaaaa
     4261 tataatagtg aagcagacac tcctcttaag aatttaagaa atggagcagc gggagcttta
     4321 agaaatttaa atgtagcaga gacagctaaa agaaatttat cagctttctt ctatgatgtt
     4381 ggatataagg aaggagctcc ctttaaaact tatatggaaa tgttaaattt cataaaaact
     4441 aagggatttc caatggatga ttatattagg gaatgcaaaa ctttagatga aattcaaaaa
     4501 gaaattgatt atataagaga tataaggttt gatttaaatt atgatataga tggattagtt
     4561 atagctatag atgatataag aactagagaa cttttaggat acacagttaa atttcctaag
     4621 tgggctattg catataaatt tgaagcacag gaggctacaa ctaagctttt agatgttgaa
     4681 tggaatgtag gtagaagtgg aagagtatca ccaacagcta tcttagagcc agtagagtta
     4741 gcaggagtta ctgttaagag ggctacatta aacaatatgg atgatatagc tagaaaggga
     4801 gtaagattag gagctgaagt atttgtaaga agaagcaatg atgttattcc agaaataatg
     4861 ggcgtagttc ctgaaagctt agaaggtaca aaggaaattg aagagccaaa ggtatgtcca
     4921 gcttgtggag cacatttagt tcatgaagga gttcatatct attgtgaaaa tactctagga
     4981 tgtaagcctc aaatggttaa aactatagtt cactttgcgg gtagagaagc tatgaacata
     5041 gctggttttt cagaaagaac agcagagcaa ttatttgaaa agcttgatat aagagatata
     5101 tcagatcttt ataaattaga atatgaaaaa ttattagatt tagataagtt tggcccaaag
     5161 aaagcacaaa atcttttaga tgccatagaa aaaagcaaag attgtacttt agaagcattt
     5221 ttatactcct taggaattcc taatgtagga gttaaaactg ctaaggattt agtaaagaga
     5281 tttgagtctt tagaaaacct agagaaggct acttttgagg aattagtatc tgttcaagat
     5341 gttggagata tagttgctag aagtataata gaatttttta aagaggaaag aactttaaaa
     5401 gtaataaatg aattattatc ccttggagta aatcctcatt acgagaaaaa agaagtttta
     5461 gaaagtccat ttatgggaaa aacagtagta gtaactggta ctttagaaaa ctattctaga
     5521 acatcaataa aagaaaaatt agaatcttta ggagcaaagg tttcaggaag tgtaagtaaa
     5581 aagactgatt ttgtaatagc gggagaagca gcaggttcaa agtatgacaa agctaaatct
     5641 ttaggtatta ctatattaag tgaagaagaa tttgaaaata tgatataaaa aaataaaagc
     5701 tatgtgtaat ttcacatggc ttttattttt ccactatatt tgagttttct tttatttcct
     5761 ctgatatatt tgtatcctct tctaaatttg tatcagcttt aatatcttta tctaaaaggt
     5821 tttcttttat ttcagaatat gaaactgaat cagccatagt atttaagtta tcaaatattt
     5881 tttccttatt tcttattaga gatatattgt gtgctatttt ttcaagtaaa taattcttat
     5941 actttatttg tctgtaagct ttatttgcaa ttacaaaact ccagattcct aaaaatatca
     6001 ttattaacat gaaaagtata gccacaactt gaactgctaa ggtcatttaa ctttacctcc
     6061 atataaaata ataaataaat tataccataa gaatagttat aaatagtatt aaataattat
     6121 tacattaaaa catataattt tagtaaatga taaataagga gataggtata tgttgaaatc
     6181 tttttatgtt cttatgaatt caaagaatat agaaaagtca ttaatgaaat ttaggaagat
     6241 atcaataaaa aaagtaagtc aaataataat taaagagtgt attaaagaaa aattaaatta
     6301 tgaaatcata gaaaagaata ataatgaatt tatagtagaa ataagttcta gtagaaaaaa
     6361 gactttaata gtatttcata aagcattggc tgtaacaatt tatgattact ataagtttat
     6421 acgtttaata caagatagaa agatagataa gggcgtatat attaccacag gagtttttca
     6481 acaagatgtt tataacatgg cagaaacaga tagatttata aatagaataa aacttctaaa
     6541 tggaaaagac tttgttgtta agcaaaggtg gataaagaga aagaaacatc atcatatttc
     6601 atatgacaag ttaagtttta aatccttatt ttaagggtaa aataagcatt tttatataaa
     6661 ttttaatgag tttaaattac tttcttatga ttgataagat gtacaatcct tttaccatgt
     6721 tattaatagt ttttataact atatttagaa ataaataaaa gaaataatat catttatttg
     6781 atttatttca acttatattt gtcaataatt taaaaggaaa agattattga aggagatatt
     6841 tacatgaaga aagtaatttt atttatatgt ttaataattt tattagtagt tggagtagga
     6901 aggagttatg tggatgtaac ccctgtttct aatatgaact ttaaatataa tgaagggctt
     6961 atatattctc ttgaaagttt accagatacc ataaggccta gtgaatcctt aaataaagga
     7021 gaaaaggatt tagtaagtaa tatattttca ggactagttg aggtaaatga aaaaggagaa
     7081 ccaattccag accttgctat gggatggaag gttagcgatg atggcttaga atatacattt
     7141 aaaattaatg aaaaaaataa gtggagtaat ggcacagaag tgacagcaaa ggattttcaa
     7201 atatttttta gggatttatt aagtccagat aatgaagatt atacaagtaa tgagttatat
     7261 agtatttacg gagttaagga ttatagagaa gggaaaattg attttagtga agtagcaata
     7321 agtagtcctg atgattatac attggtttta agaatgaaca ataaggatga tgatttactt
     7381 aaaaatttag ctaaacctat atatagacta agagatttaa atgaaccatt agataattat
     7441 aagagtgatt ttaatgaaat atcatatact gggccatatg ttatatatga tgtaactaag
     7501 gatggattta taagattaaa agacaatcct tataatagta gcgaattaga aataaaagat
     7561 atagctttta aggagaaaga gtctgatgaa attgagttag cagcttttaa tctagaaaaa
     7621 gtagatattt taaagaatcc tcctataata tattcagagg atataggatt atataaagat
     7681 ttaagtaaat ataatagtga tattttaaag ttaatgattg taaatagtga taaagctgaa
     7741 atagcgcagg gaatatctaa tattatgcaa gttttaatta gtgatagtaa tatattaaaa
     7801 aataatttag gaaaaccata tataaaagta ataaataaca aagataaaat tcactcaatt
     7861 aaaaaggatg gagataaagg agattataag attaatgaat cagaaaaaaa taatcttaaa
     7921 gaatcaggta aaaatacttt gaaaaaggtt tttaataata atgaggtagt aaaaatagtt
     7981 gtaggaaata atgaagaaga taaagcttta gcaaatgaac tgaaaaaatt tttagacaag
     8041 gagctagcca taaagagtat agtaaaaatt catgatgagg gtatagagaa agtaataaaa
     8101 agtggagagt ttaatatagc ttttggtgaa tttaacttaa atgaaaaaaa ctcagaaagc
     8161 ttaaaacagg aagatataaa taaagaagag gaaaaagaaa ctgctacttc agaaaagtct
     8221 aatttaagtt tagttagttt atattttaaa aatgatgttt ggtgtaagtc accaaaggtt
     8281 aattatttat ttattgattc aaatggaaat ttaatattaa aatatagcag ttaaaatact
     8341 tataatttta tatgaaagta aatttacact ctaagtatta aaaaacttta gagaaattta
     8401 ttttacataa gtaaccctaa attacaaatt gtatttaata ataaaatcta agctaataaa
     8461 atatattttc tgtcatataa atctaaatca taaaccatat ttaataaaaa acttataaat
     8521 agcacattag gaaaagaaag agtatagaaa ttcctagaaa gctatttata agtctttaat
     8581 tgttttatgg agtataaatt attataaatc agtaaaatca agaatatttt tcattttatc
     8641 atctaaatcc tcttttttat ctattgaaga atcactatat tcagatattt ctctagttaa
     8701 gtttggattt gttggtggtg gagaaatttc atcaacttga ttttcattag ttatagaatt
     8761 atagtctgat tctccagcat caggtggaac tatagtagtc ttagagttaa aattatttaa
     8821 tttaggaagt ttatttattt tttgtacatt ctttaaagag tctatttctt ctactaacat
     8881 agcacttttt ttctctttag gagtatcagt aataaaatct atatatttat caataaaact
     8941 atttataaat acaaagaata taaacatatt ttttgtacta tcaaaggtag ttttatctga
     9001 agtgaaaatt gatttctttg tagagtactt tagatttttt gtgaatatag gtgctaatcc
     9061 tgtaagttca gggctattag cattagtcat tctaaacatt gattcatcaa ttaaatcatc
     9121 aggctttaaa gagaactttt tatatctttc atgagaatct aaatttggtg catcagggaa
     9181 actagttact atgcctgagt taactatata atttttatag accttagata ttatattctc
     9241 tcttttatct gaggaatcct catctaaatc aaaggacatt aaaagattag ttaaatataa
     9301 gcatatttcc atattagaat atttatactc ttttttatct gttcctttta ggattattcc
     9361 cttttcatca ttaaacatat tacagaaaag ctcaattaag tctaagtaag catctttaaa
     9421 tataagtatg tttgtattct tatagcttaa atacatattt aaggctagta gagaagtggt
     9481 ttcaagatta gaaaatgtgc tatttgattc ataatatttg tttaatatga aatcagacaa
     9541 atctaaaact aataatctac aatcttcatt atctgaatat agaagcatgt tatttaagca
     9601 atagtctatt ttgcaacatt catctaagga aagtaagtat aattcttctt taaaattaac
     9661 aaacatgttt aatatttgaa gtgcaaaatc ttcataggtt ttgctatcag aatcttcagg
     9721 tgctacctta ctatacatat agtaagctaa catcattaat gcttgatctg agtaggagat
     9781 ttcatcttct ttttcactta tatttaagcc agacttagaa gaagactcat catttttctt
     9841 atttacaaaa aaaccttctg gatttcttaa ataggtatag taaaattcca gttgaagctt
     9901 acttaaattt ttataaaagg cagctctttt ataaagtgca ctattaatat ttttaaattt
     9961 ttcatagtaa gaacaaagtc ttaaaagaga aagggtcatt aatgcattag atgaaactgg
    10021 aatatctttt ttaaaggtgg tatcatccca agtttttgaa tacttagaag aataaatatt
    10081 aggctttgct tttttatata tacaaattaa tggggtatta tttttaaaga atgatatctc
    10141 atctttttta aaattttctt taagattttt gtaatttgta gatattccac acctagattc
    10201 aagtactata tgatttactg attcttttga aagaaaaagt aattgtgatg ccatctcttc
    10261 tttagaaaga gaattagctt tgaagaatga acctatatac ttcaaataat tcacctcatg
    10321 atattatttc ttatatatat taatatgaat ttttattata aatattacct agtgagtaca
    10381 caatactttt aggtgaaata aaggtttaga gggcttttta tagacataat ttttatatat
    10441 aatccatgtt gataaaggat aaaataacca atatactatt cttataatta gtgttttgaa
    10501 aataaattaa ggtaaggaga tattgaatga gaagtagtgg aagaaaaaaa gaacagataa
    10561 gaccagttaa aataacaaga aactttacta aatatgcaga aggctctgta ttaatagaag
    10621 ttggggatac taaagtatta tgtactgctt caatagaaga aaaagttcca ccttttttaa
    10681 agggttctgg agaggggtgg ataactgctg aatataacat gattccaaga agtactcaaa
    10741 gtagaaaaca aagagatata aataaactta aaatagatgg aagaacaatg gaaatacaaa
    10801 gacttatagg gagagctctt agatcagctg tagatatgaa agctttagga gaaaaaacta
    10861 tttggataga ctgtgatgtt cttcaagctg atgggggaac tagaacgact tctattacag
    10921 gctcatttgt ggcgctagtt gatgctgtaa ataaacttca tcaaaagaaa ccttttaatg
    10981 tttatcctat aagacatttt gtcagcgcag taagtattgg aatagtagga gaagagaagg
    11041 tattagactt atgctatgaa gaagaccatg tagctaaagt agatatgaat gttgtaatga
    11101 cagaagaggg agaatttata gaaattcaag gtactggaga gattggacct ttttcaagaa
    11161 aagaattaga tgaattatta aatcttgctg aaaaaggagc gaaacaaatg atacaagcac
    11221 aaaaaaatgc tttaaagaca gattctttat ggatcggaac agggagagag taaaatgaaa
    11281 aaatttatac tagcaagtaa taatgcacat aaggtaaaag aaataaaaga aatattaaaa
    11341 gattttaatt tgaacatatt atctttaaat gaagcaggaa tagatataga tgttgaagag
    11401 gacggaaaaa catttgaaga aaattctttt aagaaagcta atgaaataag aaagtattta
    11461 cttagcagag gtgaaagtga ttttattgta atggctgatg attcaggact agaagtagat
    11521 tatttaaatg gagcaccagg tatatattct gctagatatg caggagagca tggaaatgat
    11581 tctaaaaata atgaaaaatt attagaggaa ttaaaaaatg ttaaagatga tgaaagaaaa
    11641 gctaatttta tatgtgtaat agttgcagtt acagataaag gagaaaaaat agtagcagag
    11701 ggaaaaagtt atggagtaat tttagaagct ttaagtggaa atgaaggctt tggatatgat
    11761 cctttatttt tcgtaccaga atacaaaaaa actttcgcag aaatgacttc agatgaaaag
    11821 aatgctatta gccatagggg aagagcttta gaaaaactaa aggataattt aaaagggata
    11881 cttaaatagg aggagaagaa atgaaagtag ctatagtcag tgatacacat ggagttactc
    11941 atgttattga atatataaaa actttactaa atgattcaga tgttcttata catttaggag
    12001 ataatataaa tgatgttaag ttattaagtg aaagttttaa ggggaaggta tattctgttg
    12061 taggaaactg tgactatgaa agaaatgggc aagttgagca agttattgaa attgaaggca
    12121 aaaaatttct aataactcat ggtcataaat atacagttaa gtatggatta gataaaattt
    12181 actatagagg acttgaatta ggggtagatg gagtaatttt tggtcatact catagaaaag
    12241 tagccttaaa agaaggaaat atgtggataa taaatcctgg tagtccatca atacctaaag
    12301 atggagaagc aagtatagct tttatgaatg tagattctga aaatatagaa gtaagatttt
    12361 tttctattta attagaatta aattaaaaaa taagtgttaa tacataaaaa aaaactatgt
    12421 attagcactt tttttaaaaa gaaaacatag attttatcag ggtttcagag gattcgataa
    12481 aattttctaa aaaaacttta aaaaaagtgt tgacataata ataactatac tgtagaataa
    12541 ctattgtcgt cgcgagagag cggcggtgaa acaaagcct
//
LOCUS       DAEVGI010000068        12544 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEVGI010000068 DAEVGI010000000
VERSION     DAEVGI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12544
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.068"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(123..980)
                     /locus_tag="K8M77_001539"
     CDS             complement(123..980)
                     /locus_tag="K8M77_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI7086035.1"
                     /translation="MEDYILKVEELNYNYSDGTHALKGINMNIKRGEVTAILGGNGVG
                     KSTLFQNFNGILKPSSGRILFDNKPIDYSRKGIMKLRESIGIVFQDPDNQLFSASVYQ
                     DVSFGAVNMKLPEDEIRKRVDNALKRTGIEHLKNKPTHCLSFGQKKRVAIAGVLVMEP
                     KVLILDEPTAGLDPMGVSEIMKLLVEMQKELGITIIIATHDIDIVPLYCDNVFVMKEG
                     RVILQGNPKEVFAEKEVIRKVNLRLPRIGHLMEILKEKDGFVFDELDLTIGQARKTIN
                     SWKNKIFND"
     gene            complement(995..1765)
                     /gene="cbiQ"
                     /locus_tag="K8M77_001540"
     CDS             complement(995..1765)
                     /gene="cbiQ"
                     /locus_tag="K8M77_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBI7086036.1"
                     /translation="MINIDKYAYASKLKNINPAEKMFFALITLCVCLWANNFIVSMLI
                     ISIMTFLITKVGRTKIKVFIKLMMVPIAFLIIGILTIVVSYSLKQDTFLVSFKLFNGW
                     IGVSKSGIIQGLNMFLKVMGSLSCLYFISLTTPMIDVISVLAKLKVPSFLIELISLVY
                     RFIFIILETSEMMFISQNSRLGYRDIKTSYKSLGALASTLFIRSFKRANDLYTALEAR
                     GYNGELKVLEEQVQKRNYIYIITIIINLIFIGIAIFIK"
     gene            complement(1749..2060)
                     /locus_tag="K8M77_001541"
     CDS             complement(1749..2060)
                     /locus_tag="K8M77_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI7086037.1"
                     /translation="MKNKRVLTNVILLLLVVFITIIPFFVAKNGEFGGSDDQAEEAIT
                     QIDKNYEPWFSPLFEPASGEIESLLFALQAAIGAGVIGFGLGYLKGKKKVNDEVNDKH
                     R"
     gene            complement(2060..2803)
                     /locus_tag="K8M77_001542"
     CDS             complement(2060..2803)
                     /locus_tag="K8M77_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBI7086038.1"
                     /translation="MSKKNQVFIGFLLMFLLIPRSVSAMHIMEGFLQPKWCIAWGVIT
                     IPFIVLGLFSIKNKVGNNPRLKILLAMAGAYAFVLSALKLPSVTGSCSHPTGVGLAAI
                     LFGPTAASVLGLIVLLFQALLLAHGGLTTLGANTFSMAVVGPLVSYGLYKLVKKLNGP
                     TWLGVFLASALGDLATYMVTSVQLGLAFPAEVGGIMASVMKFMGIFAVTQIPLAISEG
                     ILTVIIFNTIENYAKEDLMELNIFSKGVK"
     regulatory      complement(3090..3291)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(3680..5701)
                     /gene="nagH"
                     /locus_tag="K8M77_001543"
                     /pseudo
     CDS             complement(3680..5701)
                     /gene="nagH"
                     /locus_tag="K8M77_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009663.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronoglucosaminidase"
     gene            6077..6466
                     /locus_tag="K8M77_001544"
     CDS             6077..6466
                     /locus_tag="K8M77_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086039.1"
                     /translation="MKKGNVRTLDGIIYSLFIFMTIVTLFIIYRDIPSNAVLNFVTAY
                     AIFAILIPLYILIRVLFTVKHFTFEEVKKEGVKFLMFFIGIMMLNLVGDFLLPLPDLK
                     LSKMLPSSLVFAFGITCFDIVLSKRTK"
     gene            complement(6654..7493)
                     /locus_tag="K8M77_001545"
     CDS             complement(6654..7493)
                     /locus_tag="K8M77_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBI7086040.1"
                     /translation="MGILEQLENPKFKATKSEKTLIEYIKSDLDNIIYKSISIIAKES
                     GVGEATITRFTKKLGFNGFQDFKVTLAKEISNKKNTSIINLHVHRDESVTETANKMLK
                     SSINILEQTVKQIDLDLMCKCRDLIMNAKRVYFIGIGYSGIAATDINYKFMRIGFTTV
                     PVTDSHTMVIMSSITNDDDVIVAISNSGTTKEVIKTVKQAKENGTKIITLTEDSDNPL
                     RKLSDYELTYKSAETIFETGSISSKIPQIFLLDLLYTEVIKEMFSEAVEKKIKTTSAI
                     LND"
     gene            complement(7684..8571)
                     /locus_tag="K8M77_001546"
     CDS             complement(7684..8571)
                     /locus_tag="K8M77_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBI7086041.1"
                     /translation="MGLFAVIDIGGTSIKYGVINEDGTLLETNDRDTEAYKGGLSIID
                     KVKDIIHEIKINNDIRGICVSTAGMVCPKEGKIVYAGPTIPNYTGVEVKKILEEEFNL
                     PCFVENDVNCAALGEFFGGAGKGTHSMACFTIGTGIGGALIIDGKILHGFSNSAGEIG
                     YMMVNGEHIQDIASASALVKNVALRKGVESSSIDGRYVLDNYENGDLICKEEVEKLAD
                     NLALGISNIVYLINPEVVVLGGGIMAREEVFRPLIENSLRKYLIESVYNNTKIAFAKL
                     KNTAGMKGAYYNFKENFNK"
     gene            complement(8727..9179)
                     /locus_tag="K8M77_001547"
     CDS             complement(8727..9179)
                     /locus_tag="K8M77_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI7086042.1"
                     /translation="MIFGNLINLESYDYLPKLVKEALLHAKNNNVIEFEPGTHEIKGE
                     ELFFNRVNYTTKNKEDRFWEGHKKYIDVHVVFSGRERIDLNFKENLEFKEFVEKDDFL
                     SFDGKENSTVVLEEGDFLICYPEDIHMTCLKADNEPSDVKKAIYKVIL"
     gene            complement(9277..10794)
                     /locus_tag="K8M77_001548"
     CDS             complement(9277..10794)
                     /locus_tag="K8M77_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBI7086043.1"
                     /translation="MQGFTKFDLVVLVVYLGAVLYAGLKFSKKEMKGKEFFRGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYKGTWIMWFAQLGMFVAVPLTIRYFLPVYSRLN
                     IDTAYEYLEIRYKDKGLRILGAIMFIIYQIGRMAIIMYLPSMVLSNLTGINVYLLIVA
                     MGVIAIIYSYTGGLKSVLWTDFIQGCVLIAGIIFSLIFLISTINGGFGAIMHELTEGG
                     KFLASKEPIFNANIFKTSVFLLIVGGGLNTFSSYISSQDIVQRFTTTTDIKQLKKMTY
                     GNLVLSLFVATVFYLIGTALYVFYNQNPQLLQTAQQDQIFASYIAFQLPVGITGIVLA
                     AIYAAAQSTLSTGLNSVATSWTLDIQNFITKDMSMEKQTKVAQYISLGVGIASIIMAV
                     ILANGGVNSAYEWFNGFMGLVLGVLAGIFVLGIFFKKATKAGAYAGFIASAIAVIVCK
                     YGGFDVSIWSYSIISIGVSVIVGMIVSLMTQSQLADKKTLNEATIYYNENEIVNN"
     gene            complement(10896..11762)
                     /locus_tag="K8M77_001549"
     CDS             complement(10896..11762)
                     /locus_tag="K8M77_001549"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBI7086044.1"
                     /translation="MKGIYSALLVSFDKDGNINEKGLREIIRHNIDVCKIDGLYVGGS
                     TGENFMLSTDEKKRIFEIAMDEAKGQVKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFNEIKHYYETIINSIDNKLIIYSIPFLTGVNMSIEQFAELFENDKIIGV
                     KFTAADFYLLERMRKAFPDKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQIFE
                     AAQKGDIETALEVQHVTNDLITDILNNGLYQTIKLILQEQGVDAGYCRQPMKEATEEM
                     IAKAKEINKKYF"
     gene            complement(11799..12464)
                     /locus_tag="K8M77_001550"
     CDS             complement(11799..12464)
                     /locus_tag="K8M77_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBI7086045.1"
                     /translation="MLDGVKGNLIVSCQALSDEPLHSSFIMGRMAIAAKQGGAAAIRA
                     QGVNDINEIKEVTKLPIIGIIKRNYDDSEIYITPTMKEVDELLKTDCEMIALDATKRK
                     RPNGENVKDLVDAIHAKGRLAMADISTLEEGIEAEKLGFDCVSTTLSGYTPYSKQSNS
                     VDFELLEELVKTVKIPVICEGRINTPEELKKALDLGAYSAVVGGAITRPQQITKRFTD
                     ILK"
BASE COUNT         4117 a         2225 c         1353 g         4849 t
ORIGIN      
        1 taaaaaaaat aaatttttat ataattattc ttatactaaa atactattat aaattaataa
       61 aaggcttata ccaaatcaat ttggtataag cctttcttgc ttaatttaat ctaacttaaa
      121 tattaatcat taaaaatttt gtttttccaa gaatttattg tctttctagc ctgtccaata
      181 gttaaatcta actcatcaaa tacaaagcca tccttctctt ttagaatctc cataagatgt
      241 ccgattctag gtaatcttaa gttaactttt cttataactt ctttttcagc gaatacctct
      301 ttaggatttc cttgtaatat tactcttcct tctttcataa caaaaacatt atcacaatat
      361 aaaggaacaa tatctatatc gtgagttgct attattattg ttatgccaag ctccttttgc
      421 atttctacta atagcttcat tatctctgaa acgcccattg gatctaaacc tgccgttggt
      481 tcatctagta ttaaaacctt tggctccata actagtaccc ctgcaatggc tacccttttc
      541 ttttgaccaa agcttaaaca atgagttggc ttatttttta agtgttctat accagttctt
      601 tttagtgcat tatctactct ttttcttatt tcatcttctg gtaatttcat attaacagca
      661 ccaaaggaaa catcttggta aacacttgct gagaaaagtt gattatcagg atcctgaaaa
      721 acaattccta tagattctct aagtttcatt attccttttc ttgaataatc tataggttta
      781 ttgtcaaaaa gtattcttcc tgaagaaggc tttaatatac cattgaagtt ttgaaataaa
      841 gttgattttc caacaccatt tccacctaat atagccgtaa cttctcctct ttttatattc
      901 atatttatac cttttagagc atgagttcca tctgaataat tataattaag ctcttctact
      961 tttaatatat agtcttccat aaaatcctcc tactctactt aataaatata gctattccta
     1021 taaaaataag attaattatt atagttataa tgtaaatgta atttcttttt tgaacctgtt
     1081 cttctagaac tttaagttct ccattatatc ctctggcctc taaggcagta tataaatcat
     1141 tagctctctt aaatgaacgt ataaatagtg ttgatgcaag agcacctaaa gatttatatg
     1201 atgtttttat atctctatat ccaagtcttg aattttgcga tataaacatc atttctgaag
     1261 tttctaaaat tataaatata aatctgtaca ctaagcttat taactcaatt aaaaaacttg
     1321 gtactttaag cttagctaaa actgatatta catctatcat tggagtggtt agggatataa
     1381 agtataagca acttaaagat cccattacct taagaaacat attgagtcct tgaataattc
     1441 cactttttga aactccaatc catccattaa aaagtttaaa tgatactaaa aatgtatcct
     1501 gttttaatga ataactaaca acaattgtta ggataccaat tattaaaaaa gctattggta
     1561 ccatcattaa ctttataaaa acctttattt ttgttcttcc tacttttgtt attaaaaaag
     1621 tcataatact tattataagc atagaaacaa taaaattatt tgcccaaaga caaacgcaca
     1681 aagtaattaa ggcaaaaaac atcttttccg ctggattaat attttttaac ttagatgcat
     1741 aagcatattt atctatgttt atcatttact tcgtcgttca ctttcttttt accttttaga
     1801 tatcctaatc caaaaccaat tacccctgca cctattgcag cttgaagagc aaataataaa
     1861 ctttctattt ctccactagc tggttcaaat aatggtgaga accagggttc atagttttta
     1921 tcaatttgtg ttattgcttc ttctgcttga tcatcagatc caccaaactc tccattttta
     1981 gctacaaaga aagggattat tgtaataaat acaactaata acaataatat cacattagtt
     2041 aacactcttt tatttttcat tattttaccc cctttgaaaa aatgttaagt tccattaaat
     2101 cttcttttgc ataattttct attgtattaa agattatgac tgttaatatt ccttcactta
     2161 tagctaaagg aatttgagta acggcaaata tacccatgaa tttcataact gatgccataa
     2221 ttcctccaac ctctgctggg aaagctagtc ctaattgaac tgaagtaacc atataagttg
     2281 ctaaatctcc aagagcagat gctaaaaata cacctaacca tgttggtcca ttaagttttt
     2341 ttactaattt ataaagtccg tatgatacta aaggcccaac tacagccatt gaaaaagtat
     2401 tagctccaag agtagtaaga ccaccatgag ctaaaagtaa tgcttggaat aaaagtacta
     2461 taagtcctaa aactgatgct gctgtaggtc caaataatat tgccgcaaga ccaacccctg
     2521 taggatgtga gcaactacct gtaactgatg gaagctttaa tgctgaaaga acaaaagcat
     2581 aagctcccgc catagctaac aaaatcttta gtcttggatt atttccaacc ttgtttttaa
     2641 tagagaatag tcctaatact ataaatggta tagttataac tccccatgct atacaccatt
     2701 taggttgcaa aaatccttcc atgatatgca tagctgaaac acttcttggt attaataaga
     2761 acattaataa aaaccctata aatacttgat tcttttttga cataacaatt ctactcctta
     2821 aattatattt ttattttgta ctcccattta tcatcttttt attttaagat aaaatttaat
     2881 ttaactattt ccgcatcaaa taaaacaata tgtactaaaa atatttccct ttataacact
     2941 taatctaaat atgaataact ttaatttcta gttaaatcag aactttagtc ttagaaataa
     3001 aaaaccccta gacattgcgt ctagaggtta taaaaattct aaaaaaatat aacttaccaa
     3061 agcacaacac ctcctattcc ccgtaggatc ctgatgtttt aaaaataggc aggtcttctg
     3121 gctcaagtat catcactttt ttgtagcctt cccaaaatat tatattccag tggcttaaag
     3181 tacaaaaagc tcctctatta cagcggcggg accgcgtggg atttaaaccc aacttccctt
     3241 ttaatatata gttataaatt taactattta acctatttct aaatatataa ttttataata
     3301 ctttaacatt atatagcata tgcattgcat ttacaattaa actctataga tttctttaga
     3361 aaatatactc tatataaggc tttcggtaat ttttaaacat ataattcctg ttcatatatt
     3421 attatgaaag attaattatt aaaaaactta tctgtttatt atataaattt aatcttaaaa
     3481 cttatcttaa attaatctga ttctaataat attcaaaaaa tttttttaaa ttctgtttta
     3541 aattaaaagg aatcatatta gatacaaata atcattttaa aatactattc tttaaaattt
     3601 ttagtatttt aaatttgatt ttaagtatct ttatgattcc taaaaaatta taaatctaat
     3661 ttatcattat taaattatct taatttagta ttctatgatt tataaagctt atctcatatt
     3721 catcaataga gccatcttta tttaagtcaa gagaagtgtt agtagtactt ctaatgtttt
     3781 tagatatcat tgctaagtct cctatatcaa tctttccatt accattaaag tctcctgtac
     3841 tttcttctat taataaagaa ttaaatcttg ctactgcacc ctctaaagat tccttagcta
     3901 aatttatttc ctcttcagta gcatcttcct tgttaaatac ttcttcagcc ttattaattt
     3961 caactgttaa tccatcctta gcacctttat ggtattcacc aacattaaat ccttctacag
     4021 attcctctaa taaagattta ccttgattta ttaattcacc taaatttgtt ttatcaacct
     4081 ctggtatttc ctttactaaa gcttttattg aagcatctaa atcacttact atttgattta
     4141 tttgattttg agtgtaataa gctctatttt tgttagcttc attagaaact tctaagactt
     4201 cactaaataa agcccatgac tcagctgtat attcttcttg aaataaagag tttcctaaat
     4261 ttaaaacctt attgaaatct tcaaagtcag ttgcccttaa agctaattct tcttctgcct
     4321 tatttaaatt ttctaaagcc ttatctattt tcttttgaga tgagttagaa tcatctaaaa
     4381 tattcttagc ttcatttaat gcttctttta aagtttcaaa gctacttaaa gtgtattcat
     4441 ctacacttaa atttataagc tcatcatatt ttgcctttaa agaatcattt actgggaatt
     4501 caaactcttg gtttaaagtt attatttcat aaatatttgg tgcaacatct gtccattcga
     4561 tttttatctc atagatatta ttgtaatcac gattgataaa ctcatttaaa ctattgctta
     4621 atgttcctaa ttgaacccat ttatcagtta cattttcact accatcacca actctagcca
     4681 taactttagc atttgaaatt gaacttccac tttgaactat tgtaactttt ctaacatctg
     4741 ttttttctga taaacgataa attatacttc cttcagaaat ctcactatta ttagtacttg
     4801 gcttatatga agttgttaaa tttccatctc ttaaattact tggggcaaag cctttttcct
     4861 caattgaact tgaaatataa gttggatcat taactgtaga gatatattct ccattattta
     4921 tcataagttc atttataact gtccaacgat gtctatatgg tgcagtaaat aatattctta
     4981 catatctagc cttttgattt aaattatctc cctccacata agcactatct attggaacta
     5041 ttccacctga ttggtttcca tgtttatatc cattttttaa tggtgtagag aacatgtcgt
     5101 ctactccatt ttctactcca tctccaatat taatagcgtc tgtctaagtt tctccatcta
     5161 aagataattg aattttacca tctcttacat gatcaacttc agtatctaat acaacatatt
     5221 ttaaattatt cacaagaatt tcttgtccta aatcatagac tatagtatca cctgtactag
     5281 gtgcacctaa gaatttaact cttgttttta aatctccatc tacagctttt ctagctccat
     5341 catctccaac ataagcatct actaatgatg attcataaac ttcatttgaa ttaacttcaa
     5401 acttagttaa attaaaatta acagtttcat tacttgtatt aattagcctc acatatctgc
     5461 cgtcttctaa tgtattttta tctgttatct cagtccactc aacaccattc atagagcttt
     5521 gaagttttaa tcctatatca tttgaaattt caagattaat attacttaaa tctttaatac
     5581 gatttaattt aaccccaata tattctccat gatttaagct taagtcatct ataggtatca
     5641 acttagttac atcttcttta gctaaagata ataaatctaa ttatgtattt ttatatacat
     5701 taaagttttc tgtaatacca tctgttgcaa aaacaactaa atttgatgga aaaacactaa
     5761 ttttcatagc tgccaatact gtagatgtaa taatctttct aatgttccta ttcataaacc
     5821 ttcccccata aagttttttc atattataac taaattgtaa ttattaccca ataaatagta
     5881 catgtatttt atttacttct tatgttaata aatgaaattt ttaacaaaag taattaatat
     5941 ctttattttt tctttagaaa gattttaggt aaatttaatg tctttgtaac tcacatgtaa
     6001 tccacttaaa ctatttaatt gttaaaattt tgtgtataat catattatag gaaagattac
     6061 aacagggggg attaaattga aaaaaggaaa tgtacgtact ttagatggaa tcatttactc
     6121 cttatttatt tttatgacta tagttacact cttcataata tatagggata ttccaagcaa
     6181 tgcagttctt aattttgtta cagcttacgc tatatttgca atacttattc ctttatacat
     6241 attaataaga gtcctattta cagttaagca ctttaccttt gaagaagtaa aaaaagaagg
     6301 agttaaattc ttaatgtttt ttataggcat catgatgctt aatttagttg gtgatttttt
     6361 attaccattg ccagacttaa aattaagtaa aatgttacct tcctcattag tctttgcttt
     6421 tggaataact tgttttgata tagttttatc taaaagaaca aagtaaaata ggtcacagct
     6481 ttattactgt gacctatttt ttattaaatt aactttaatt aatataaaac taaaactttt
     6541 tatttaactt taaataaaaa atcttgtata tcttataaac tatagatatt actaatatac
     6601 tagctctctt acaagcttat tttaagttct taattcttta ataatataca attttaatca
     6661 ttaagaattg cactagtagt cttgattttc ttttcaacag cctcactaaa catttcttta
     6721 attacctctg tatataatag atctaataaa aatatttgtg gtatttttga tgatattgat
     6781 cctgtctcaa aaattgtctc agctgattta tatgtcaatt cataatcaga taactttctt
     6841 aatggattat cactatcttc tgttaaagtt attatctttg tgccattttc tttagcttgt
     6901 tttacagttt ttattacttc tttagtagta cctgagtttg aaatagcaac tattacatca
     6961 tcatcatttg ttatagatga cataataacc attgtatggc tatcagttac tggtactgtt
     7021 gtaaatccaa ttctcatgaa tttatagttt atatctgtag cagctattcc tgaataacct
     7081 atacctatga aataaactct cttagcattc attattaaat ctctgcattt gcacattaaa
     7141 tctaaatcaa tctgcttaac tgtttgctct aatatattaa tagatgattt taacatctta
     7201 tttgcagttt ctgttacact ctcatcccta tgaacatgca aatttataat gcttgtattt
     7261 tttttgtttg agatctcctt tgctaaagta actttaaagt cttggaatcc attaaacccc
     7321 aacttcttag taaaccttgt tatggtagct tctcctactc ctgattcctt agctattata
     7381 gatattgatt tgtatataat attgtctaaa tctgacttta tatattcaat taatgtcttt
     7441 tctgacttag ttgctttaaa ttttggattt tctaattgtt ctaatattcc cataaagtac
     7501 ctcttgctat ttgaatattt tgactcaatt ctatggctat attaaatact tataaataat
     7561 ttaaaacact ctaaaactaa gtcaaatata atattttaag tatatcattc ttgtcatgta
     7621 agctccataa tttatataaa ttatattatt gttctttatt tcaagtaaaa ttacaaaaga
     7681 taactactta ttaaaattct ctttaaagtt ataataagcg cccttcattc ctgcagtatt
     7741 tttaagcttg gcaaaggcta ttttagtatt attataaact gattctatta agtattttct
     7801 taagctattt tctattaaag gtctaaacac ctcttctcta gccattattc ctccacctaa
     7861 aactaccact tcaggattta ttaaataaac aatatttgaa attcctaaag ctagattatc
     7921 agctaatttt tcaacttcct ccttgcatat taaatcacca ttttcgtagt tatctaatac
     7981 atatcttcca tctatactac tagattcaac gccttttcta agtgctacat ttttaactaa
     8041 agcacttgct gatgcaatat cttgaatatg ctctccatta accatcatat atccaatttc
     8101 tccagctgaa ttactaaaac catgtagtat tttaccatct atgattaaag ctcctccaat
     8161 acctgttcct atagtaaaac aagccattga atgagtacct ttaccagcgc ccccaaagaa
     8221 ttctcctaag gctgcacagt ttacgtcatt ttcaacgaaa caaggcaaat taaactcttc
     8281 ttctaatatt ttcttaactt caacaccagt ataattaggt attgttggac cagcataaac
     8341 aattttccct tccttaggac ataccattcc tgctgttgat acacatattc ctcttatgtc
     8401 attattaatc ttaatctcat ggattatatc tttaactttg tctattatac taagtccacc
     8461 tttatacgct tcagtgtctc tatcattagt ctctaataat gttccatctt catttataac
     8521 tccgtatttt atactagttc cacctatatc tataactgca aataaaccca tacatatcac
     8581 ctctaataaa attattattc tctaagtcta ttataaacaa taaaggttgc accaaaaata
     8641 acttaaaata aaaattacaa acctaaataa ttctagatcc tcggtttatt actttaagct
     8701 ttgatacaac ctcataaaat aaaatactat aaaattactt tataaatagc tttctttaca
     8761 tctgaaggct cattatcagc ctttaaacaa gtcatgtgta tatcttctgg ataacagata
     8821 aggaaatctc cctcttctaa aacaactgta gaattttctt tgccatcaaa agataagaaa
     8881 tcatcttttt caacgaactc tttaaactct aaattttctt taaagttaag atctattctt
     8941 tctcttccac taaaaaccac atgaacatct atatatttct tatgaccttc ccaaaaacga
     9001 tcttctttat tttttgtagt gtaattaact ctattaaaga acaactcttc accttttatt
     9061 tcatgagtac ctggttcaaa ctctattaca ttattatttt ttgcatgtag taaagcttcc
     9121 ttaacaagtt ttggtagata atcataactt tctaagttta ttaaattgcc gaatatcatt
     9181 tcattcacct ctaaaacatt caattttaaa gtagtgctga tctttaacta tctaaagatc
     9241 aacacttact ctaattgatc attattttta tttacattaa ttattaacta tttcattttc
     9301 attgtagtaa attgttgctt catttaaagt ctttttatca gctaattgag attgagtcat
     9361 tagtgaaact atcattccaa ctataactga aacacctatt gatatgattg agtatgacca
     9421 gatacttaca tcaaatccac catatttaca tactataaca gctatagctg atgctataaa
     9481 tccagcatat gcacctgctt tcgtagcttt tttgaagaat attcctaata cgaaaattcc
     9541 tgctaaaact ccaagaacaa gtcccataaa tccgttaaac cattcgtaag ctgagtttac
     9601 tccaccattt gcaaggatta ccgccataat tattgaagct ataccaacac ctaatgatat
     9661 atattgagca acttttgttt gcttttccat tgacatatct tttgttataa agttttgaat
     9721 atctaaagtc cagcttgttg caactgagtt taatccagtt gaaagtgttg attgagctgc
     9781 cgcatatata gccgctaaaa ctatacctgt tataccaact ggcaattgaa atgctatata
     9841 tgatgcaaaa atttggtctt gttgagcagt ttgtaataac tgtggatttt gattatagaa
     9901 aacatataaa gctgttccta ttaaatagaa tacagttgct acaaataatg ataatactaa
     9961 gttaccataa gtcattttct ttaattgttt tatatctgta gtagtagtaa acctttgcac
    10021 tatatcctgg cttgaaatat atgatgagaa agtatttaat cctcctccaa ctattaataa
    10081 gaatacactt gttttaaata tatttgcatt gaatattggt tcttttgaag ctaagaattt
    10141 tccaccttca gttaattcgt gcataatagc tccaaatcca ccattgattg ttgaaattaa
    10201 gaatattaat gaaaaaataa tacctgctat aagtacacaa ccttgaataa agtctgtcca
    10261 taaaactgac tttaatccac ctgtgtatga gtaaattatt gctattacgc ccattgctac
    10321 tataagtaaa taaacattta ttcctgtaag atttgataat accattgatg gtaaatacat
    10381 tatgattgcc atacgtccta tttgataaat aatgaacatt atagctccta gaattctaag
    10441 ccctttatct ttatatctta tttctagata ttcgtaagct gtatctatat ttagtctact
    10501 ataaactggt aagaaatatc ttattgtaag aggtactgct acaaacatac caagttgagc
    10561 aaaccacatt atccaagttc ccttataaga gttaccagct agtgataaga atgaaattgg
    10621 acttaataaa gttgcaaata tagaaactga agtaacccac caaggtatag ttccgtcacc
    10681 tctgaagaac tctttacctt tcatttcctt cttagagaac tttaaccctg cgtaaagaac
    10741 agctcctaaa taaacaacta gtacaactaa atcaaacttc gtaaaccctt gcataccaat
    10801 tctcccctct taaattttta tgtaggctaa ctttttaagt tagcctacta tttcctctct
    10861 cttaaaaact cactataatt taaaattttt cattattaga aatatttttt attaatttcc
    10921 ttagcttttg caatcatttc ttcagtagct tctttcatag gttgtctaca atatcccgca
    10981 tcgacacctt gctcttgaag aattaatttt attgtttggt aaagaccatt atttaagata
    11041 tcagttatta aatcattagt tacatgttga acttctaaag cagtttcaat gtcacctttt
    11101 tgagcagctt caaagatttg tcttgctcta ataccattta cgttaaatgt tgatcctata
    11161 gctccatcaa caccaagtac tgtagctggt aacatcattt catcaaatcc agcaaatatt
    11221 aatttatctg ggaatgcttt tctcatacgt tctaataagt agaagtctgc tgctgtgaat
    11281 ttaacaccaa taatcttatc gttttcaaaa agctcagcaa attgttctat tgacatattt
    11341 actcctgtta agaaaggaat tgagtaaatt ataagtttat tgtcaataga atttattatt
    11401 gtttcatagt aatgttttat ttcattaaaa tcgaatttat agtagaatgg tgtaactgct
    11461 gatattgcat catatcctaa atctgtagta aatttagcta actctacagc ttcttttaaa
    11521 ttaactgatc ctacttgagc tattaattta acttgtcctt ttgcttcgtc catagcaatt
    11581 tcaaatattc ttttcttttc atcagttgaa agcatgaagt tttctccagt acttcctcct
    11641 acataaagcc cgtctatttt acatacatca atattgtgtc taattatttc tcttaaacct
    11701 ttctcattga tgtttccatc cttgtcaaat gaaaccaata atgctgaata aatacctttc
    11761 attgtttttc tcctcccaaa atcctttaaa tatttgattt attttaaaat atcagtaaat
    11821 ctcttagtta tttgttgtgg tcttgttata gcccctccaa ccactgctga atatgcacct
    11881 aaatcaagtg ctttctttag ttcttctgga gtgtttattc ttccctcaca aataactgga
    11941 atttttaccg tttttactaa ctcttctaaa agttcaaagt caacactgtt actttgctta
    12001 gaatatggtg tatatcctga aagtgttgtg gatacacaat caaatcctaa cttttctgct
    12061 tcaatacctt cttctaaagt tgatatatca gccattgcta atcttccttt tgcgtgaata
    12121 gcatcaacta aatcctttac attttcacca ttaggtcttt ttctctttgt agcatctaat
    12181 gctatcattt cacaatctgt ttttaaaagc tcgtccactt ccttcatagt tggagttata
    12241 taaatttctg aatcatcgta atttcttttt attattccaa tgataggaag tttagtaact
    12301 tcttttattt cattaatatc attaacgcct tgtgctctta tggccgctgc tccaccttgc
    12361 ttagctgcta tagccatcct tcccataata aaagaactat gcaaaggttc atctgataaa
    12421 gcttggcaag aaactattaa atttcctttt accccgtcta acattttatc accttttttc
    12481 tgaaaattat tttctttatg tttttattgt aatccttttc ccacagaatt tcaatacttt
    12541 ttga
//
LOCUS       DAEVGI010000069        12318 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEVGI010000069 DAEVGI010000000
VERSION     DAEVGI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 12318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12318
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.069"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(205..1218)
                     /locus_tag="K8M77_001551"
     CDS             complement(205..1218)
                     /locus_tag="K8M77_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7086046.1"
                     /translation="MNLRDFIRERIVFIIINLLILLFTAVLLMVLKVDSFAILFIIII
                     NGAGILIYHIFDYLRKKQYYDEIKENMESLDKKYLISEVIEEGTFTESKLIYEVICKS
                     NKAMNDEIGEFKRGINDYREYIELWVHEIKTPIATCKLLIENNESPLTESIGEEVCKL
                     ENYIDQALFYTRSNTIEKDYIIKEMSLSSCVNKVLNNNADALIKKRVKISLGDLEKIV
                     YSDSKWIEFILGQIISNSIKYMNKEHKELKIYCSESSKYVILNIEDNGAGISEKDISR
                     VFDKGFTGENGRKFGKSTGIGLYLCKKLCKKLGLDIKLISEEGKFTRVSIIFPINRMM
                     IFE"
     gene            complement(1215..1895)
                     /locus_tag="K8M77_001552"
     CDS             complement(1215..1895)
                     /locus_tag="K8M77_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7086047.1"
                     /translation="MESKKIFIIEDEEKIREELTKFLEKYGYVVESSDDFENIVDISL
                     SCNPNMILLDINLPYYDGYYICREIRKKSQVPIIVVTSRQSEMDELMSMNLGADDFVT
                     KPYNTQILLARIASIMKRTYSGIEGETEIFNCRGLKYNMSTSEVSFKGEVEELTKNES
                     KILNILIRNKGKIVSRDNIIKTLWQSNEFIDDNTLTVNVNRLRRKIEGIGAEGYLQTK
                     RGQGYILS"
     gene            complement(2035..2835)
                     /locus_tag="K8M77_001553"
     CDS             complement(2035..2835)
                     /locus_tag="K8M77_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI7086048.1"
                     /translation="MFCSKCGKEIPNESVFCPECGNRCKGSNKSIPIDVNEHKGYIIK
                     SLCHPITSVKEHVFGVSLKIQLIYIAIITLIIPLVKTFLFKAFSFNLVKSVVGIILEF
                     SSVNTWDFNNMIKAKSQFDMIMENVFPTGNIYFLNLGSYVINYVLMLGIIYIIFKFLV
                     KEDINKDSLVNIMFVVSIINIFILIISSIALVLGGAIWMIVSIFSSILSVVLLYALFD
                     TLIKSKNKFIYIYSLTCMVVYSINAWFVFNNVSSIIYQIYYNLERYFI"
     gene            2951..4201
                     /locus_tag="K8M77_001554"
     CDS             2951..4201
                     /locus_tag="K8M77_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086049.1"
                     /translation="MNFCNKCGSKLINGVCPNCSKIKKNKKKSKIIIISLVLIVVIFS
                     GVFFYLKSTVKSEKEIALSFSNSISSSNPEELSKILYCNDSSLPINKSNSTILIDYFN
                     QNPSKFSSINDDFKKGNYTDTDSPLSIEEVRKDFFLIPVYKVVVKPSFIKVKTDLKDA
                     KVQIGDETFGDLTKKDELGPLMPGNYTIKSEISNSYLNKSENIEVNTFKSSNQEISIF
                     DNFIKVNITSDIPDAELYVNNKDTGVKIKDAKTFGPIDPNSIIYGVAKDGDKKIISNK
                     YDVNYSKNININFAEAKASEANFKKDLYVLLSNYSNDFAYAVNTNSFNYIENYLEFDS
                     PIYNKQKKVVPEIHSKDIRENFESTEILNYTFNNDTNTGEVTCNEIYSIGKGINVPKR
                     QEFKNTYTFKKLSNGSLVLTDIKD"
     gene            4213..5385
                     /locus_tag="K8M77_001555"
     CDS             4213..5385
                     /locus_tag="K8M77_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HBI7086050.1"
                     /translation="MKFCSKCGNKLSDSMKFCNKCGAKVKSVDKDTENNSNPEDAKNI
                     SNIENNIDKTIILDAPKIKKEINTDINENIKNNLKPIHNSNFNNKEKCLDASFDDDLD
                     DDLDENFYDESCNNENTDNFKHSNTKKKILISIFTLVAFVIIGTSIYFLRSPLLYKYY
                     YNSALKSSSVTEKLSYYNSALKYSKNDDLLNSIYTTLKSDSDFVDNSSILTNLNKSEK
                     DNLMSKLYVNKATVDFKNKNYTDCDSDLDLATKYGYKKENFSQYDDLQKKLNESKNSS
                     NNDKVDNVYSFTNENPSKFSGNIYDYPYDFIMPYSNSSYLSASDLSKYNKSTLALMRN
                     EIYARHGYVFNTNPFKEYFNSKSWYHPDSSFKGDDSELNDYEIKNVQTIKSVENSK"
     gene            5398..6357
                     /locus_tag="K8M77_001556"
     CDS             5398..6357
                     /locus_tag="K8M77_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7086051.1"
                     /translation="MKNFLRILSIIIISSLMLLGCGSNSTKPNINKDSNSINNSNEEN
                     NKIEKNNSNNKNNSIDENNNKEKNDSKEGNIDQKKEKNKIDSNTSKETDKKSDVEPYC
                     ENEPNKANKDITIVIDPGHSSTISNETEPECPGSQKRKLKDTLGAIGVQSKIPEYTIT
                     HGVAEELKKLLTSEGYNVIMTKDSPDKQLSNIERTTIGNDNNANLIIRIHCDGVDSPK
                     ACGASILVPATKGNVTKDISDISYSYGEKILTAYTKYTGLKNRGVVVRDDLTGFNWSK
                     VPIVLIELGFISNPNEDLYLSNPDNYIKIATGISNGINYCFAK"
     gene            complement(6417..6641)
                     /locus_tag="K8M77_001557"
     CDS             complement(6417..6641)
                     /locus_tag="K8M77_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBI7086052.1"
                     /translation="MEKELYLVLSFKNAGGSITKITLKNIKEDVTEEEVQNLMEKIVT
                     ANIFASKGGDLVSKVKGEIVEKTTESFEMA"
     gene            complement(6925..7152)
                     /locus_tag="K8M77_001558"
     CDS             complement(6925..7152)
                     /locus_tag="K8M77_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBI7086053.1"
                     /translation="MAKAILTKKSLVLKYQKGVDDSGSPKFSTQKFSKIKVDAEDEKL
                     YEVGKALADLLSSRVNSVLKEDDFKFVDDTQ"
     gene            complement(7301..8863)
                     /locus_tag="K8M77_001559"
     CDS             complement(7301..8863)
                     /locus_tag="K8M77_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBI7086054.1"
                     /translation="MKKNKLITAIILAGAISIGSFTTVFADTKEVVTLGANLNSTQKQ
                     EMFKEFGVKPNDVKVITMNVNEIREQLGLPKIVGEFKGNAYSSVFVKIEEKGYGLKVK
                     TNNLTEVTKTMLSNALLTSGVTDADVIATAPFPVTGTSALAGVLQAFEKATGENIPVE
                     NKEVARQELSITNNLAKAKNSEGQDIGRDGASAIVNQAKEEVIKDKPKNDKEVGEIVN
                     NITNNYNIELTPTQEQELVGLLANINSLGLDYSKLKGELDSLSNNIQETLKENGQELK
                     ECGTLDRILNKILGVCTDIKNWFVAHLGDEEVTINGVTYDKDGNMINKDQLKNIGTSI
                     SDDDNDTSNNESKNNTEDKSQKSDSKDKEIDKANSNKQDASNSEGEQESKSNSSLNND
                     NKGNKESQNNNAKGDKASQNSKSKNGVSSSKNNSNKNNSGSSNNSKDKSKETIKLEDG
                     TVIPKYNSKGEEYNPVTGGYGHMQETEDAGKDSDQTIDYIIVDGKKVPLHDEQGREYN
                     PETGRYGDPDDN"
     gene            9307..9558
                     /locus_tag="K8M77_001560"
     CDS             9307..9558
                     /locus_tag="K8M77_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBI7086055.1"
                     /translation="MSTRIAVVGIVIESLDNIKEVNSILNKYEKLIVGRMGLPYKEKN
                     IRVISIVVDGTTDEISAITGKIGKLKGVSVKAAISKTSF"
     gene            9664..11085
                     /gene="hydG"
                     /locus_tag="K8M77_001561"
     CDS             9664..11085
                     /gene="hydG"
                     /locus_tag="K8M77_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBI7086056.1"
                     /translation="MLKDNEKYNALDFIKDDEINSLIAKGKELVYDKELVREIIEKSK
                     SAEGLTPEETAVLLNLEDKELIEEMFKAARQVKEKLYGKRLVVFAPLYVSNYCVNNCT
                     YCGYKHCNDELKRKKLNKEQLIEEVKVLESLGHKRIALEAGEDPVNAPLDYILDCIKS
                     IYSIKFDNGSIRRINVNIAATTVEDYKRLKDAEIGTYILFQETYHKPTYERLHVSGPK
                     HNYNYHTTAMHRAREAGIDDIGMGVLYGLYDYKYETLAMLMHAMDLEETTGVGPHTLS
                     VPRIRPAENVSLENYPYLVDDEDFKKIVAILRLAVPYAGLILSTREEPGLRDEIIALG
                     VSQVSTGSCTGVGGYSESYVDPEEKPQFEVEDHRSPVEMIESLMEAGYIPSYCTACYR
                     EGRTGERFMEIVKSGELYKICEANALITLKEFIDDYGTDKTREIGDKLIKKSIDEIDN
                     ESFRKSVEEKINKISKGTRDLRF"
     gene            11764..12048
                     /locus_tag="K8M77_001562"
     CDS             11764..12048
                     /locus_tag="K8M77_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086057.1"
                     /translation="MTVDQNSLERRTCCNIHKIVLTLEKGFSSMESLKYAVITHLQNE
                     GYRCNDTKNNIGDYPLLKINANDFLLTIKDIDENSLNFEGCSEVVLVPHH"
BASE COUNT         4566 a         1802 c         1391 g         4559 t
ORIGIN      
        1 gaattataac taaaaaagca ttaacaaatg tcttaatata ataaaaattt atattcttta
       61 aaaattcaat atttaatata atagcttcta ttatgttaag tgaacattta ataaaaatac
      121 atactataac tacaacccaa aacttttacc catatattct ccccctcact atttaaatca
      181 tcataagaat aaatccatta ttccctattc aaaaatcatc atcctattta ttgggaagat
      241 aattgaaact cttgtgaact taccttcttc tgaaatcaat tttatatcta aaccaagttt
      301 cttacaaagc tttttacata ggtatagtcc tatacctgta gactttccaa acttacgtcc
      361 attttctcct gtaaatcctt tatcaaaaac cctagaaata tctttttcac ttattccagc
      421 accattgtcc tcaatattta aaatcacata tttgctactt tcactgcaat atatctttag
      481 ttccttatgc tccttattca tatatttaat agaatttgat attatttgcc ctaaaataaa
      541 ctcaatccac ttactatctg aataaactat tttttccaaa tcacctaaac ttattttaac
      601 tcttttcttt attaaagcat ctgcattatt gtttaaaact ttgtttacgc aactacttaa
      661 actcatttct tttattatat aatctttttc aattgtatta cttctagtat aaaagagagc
      721 ttgatctata taattttcta gcttacaaac ctcttctcca atactttctg taagtggaga
      781 ttcattattt tctattaata gcttacaagt tgctatggga gtttttattt catgaaccca
      841 tagttctata tactctctat aatcatttat ccctctctta aattccccta tttcatcatt
      901 catagcctta ttactcttgc atataacctc atatattaac ttactctctg taaaagttcc
      961 ctcttctatt acttctgata ttaaatattt cttatctaaa gactccatat tttcctttat
     1021 ctcatcataa tattgcttct tccttaaata atcaaaaata tgataaatta aaatccctgc
     1081 cccatttata ataataataa ataaaatagc aaagctatca acctttaaaa ccataagcaa
     1141 tacagctgta aaaagtagta ttaataaatt tataataata aaaactattc tctctctaat
     1201 aaaatccctt aaattcatga taatatatac ccttgccccc tcttagtttg taaatatccc
     1261 tctgccccta ttccttcaat ctttcttcta agtctattta cattaacagt taaagtatta
     1321 tcatctataa actcattact ttgccataaa gtctttataa tattatccct tgatactatt
     1381 ttccctttat ttcttattag aatatttaaa atcttacttt catttttagt aagctcctca
     1441 acttccccct taaatgaaac ctcactagta gacatattgt attttaatcc tctacaatta
     1501 aatatttctg tttccccctc aattcctgaa taggttcttt tcataattga tgcaattcta
     1561 gctaaaagta tttgagtatt ataaggctta gtaacaaagt catcagcccc taaattcata
     1621 ctcataagct catccatctc actttgtcta ctagttacaa ctataattgg aacttggctt
     1681 ttcttcctta tctccctgca aatatagtaa ccatcataat aagggagatt tatatctagt
     1741 aaaatcatat taggattaca acttaatgag atatcaacta tattttcaaa gtcatctgat
     1801 gattctacaa catagccata cttctctaaa aatttagtaa gctcttctct tatcttttct
     1861 tcatcttcta taataaaaat ctttttgctt tccatctaat ctactcccat aaaaattctt
     1921 ttaaattata aatacattta actcctattc atattctaaa atatacaatc tatataatta
     1981 ttaaaacaca aaagagacac ttataaaaag aagtgcctct ttatatatac tatattaaat
     2041 aaaatatctc tctaaattat aataaatttg ataaattata gatgatacat tattaaaaac
     2101 aaaccatgca tttattgaat aaacaaccat gcaagttaaa gaatatatgt atataaattt
     2161 attttttgat tttattaaag tatcaaataa agcatatagt aatacaactg ataatataga
     2221 actaaatata cttactatca tccaaatagc tcctcctaaa actagggcta tagaggatat
     2281 aattaatata aatatattta ttatagatac tacaaacatt atgtttacaa gtgaatcttt
     2341 atttatatct tctttaacta aaaacttaaa tataatataa attatcccta gcatcaaaac
     2401 ataattaatt acgtagcttc ctaaatttaa aaaatagata ttacctgtag gaaatacatt
     2461 ttccataatc atatcaaact gacttttagc ctttatcatg ttattaaaat cccaagtatt
     2521 aacacttgag aattctagta taatacctac tacacttttt accaagttaa aactaaaggc
     2581 tttaaataaa aaagttttaa ctagtggtat tattaaggtt attattgcaa tataaattaa
     2641 ttgtattttt aatgatactc caaagacatg ttccttaacc gaagttattg gatgacacaa
     2701 agattttatt atatatcctt tatgctcatt tacatctata ggtatacttt tattagatcc
     2761 tttacaccta tttccacact ctggacaaaa aactgattca tttggtattt ctttaccaca
     2821 ttttgaacaa aacataaaac cactcctatt ctatgttaaa ttataacttt tcgttaacaa
     2881 atttgtcaat cgttcaaaaa aatgttaaat tatattttag atgtaaaatt attattaagg
     2941 aggttttaac atgaattttt gtaataaatg cggttcaaaa ttaataaatg gagtttgtcc
     3001 caattgctca aaaataaaaa aaaacaagaa aaaatctaaa attataataa taagcctagt
     3061 tcttattgtt gtaatatttt ctggtgtatt cttttactta aaatcaacag ttaaatctga
     3121 aaaagaaata gcactttcat tttcaaattc aatttcctct tcaaatccag aggaacttag
     3181 taaaatactt tattgtaatg atagctcttt accaataaat aaaagcaact ctactattct
     3241 aatagattac tttaatcaaa atccatcaaa attttcttca ataaacgacg attttaaaaa
     3301 gggaaattat acggacacag actccccctt atctatagaa gaggttagaa aagatttctt
     3361 tttaatacct gtttataaag tagttgtaaa accaagcttt ataaaggtaa aaactgattt
     3421 aaaagatgca aaagttcaaa taggagatga aacttttgga gatttaacaa aaaaagatga
     3481 gttaggccct ttaatgcctg gaaattatac tattaaatcc gaaatatcaa attcatattt
     3541 aaacaaatct gaaaatatag aggttaacac ttttaaatct tctaaccaag aaataagtat
     3601 atttgataac tttattaaag ttaatattac tagtgatatt ccggatgctg aattatatgt
     3661 aaataataaa gatactggag taaaaattaa agatgctaaa acctttggtc caattgatcc
     3721 taatagtatt atttacggag tagcaaaaga tggagataaa aaaataataa gtaacaaata
     3781 tgatgtaaat tattcaaaaa atataaatat aaactttgct gaagctaaag cctcagaagc
     3841 taatttcaaa aaagatcttt atgttttatt aagtaattac tctaatgatt ttgcttatgc
     3901 tgttaataca aatagtttta attatataga aaactacctt gaatttgaca gtcctattta
     3961 caataaacaa aagaaagttg tccctgaaat acattctaaa gatattaggg aaaactttga
     4021 aagcactgag atattaaatt atacatttaa taatgataca aatactggtg aagttacttg
     4081 taatgaaatt tattctatag gtaaaggaat taatgttcct aagcgtcaag aatttaaaaa
     4141 tacttatacc tttaaaaaac tatctaatgg ttcattagtt cttactgata taaaagatta
     4201 aggagtgatt ttatgaaatt ttgttctaaa tgtggtaata aattatctga ttcaatgaaa
     4261 ttttgtaata agtgtggtgc taaagtaaaa tcagtagata aagatactga aaacaattca
     4321 aatccagagg atgcaaaaaa tatttctaac attgaaaata atattgataa aaccataata
     4381 ttagatgcac ctaaaataaa aaaagaaatt aatactgata ttaatgaaaa tattaaaaat
     4441 aatttgaaac ctattcacaa ctctaatttt aataataaag aaaaatgttt agatgcaagt
     4501 tttgatgatg atttagatga tgatttagat gaaaatttct atgatgaatc ttgcaataat
     4561 gaaaatactg ataattttaa acattctaat accaaaaaaa agattttaat aagtattttc
     4621 acattagtag cttttgtaat aataggtact tctatttatt ttttaagatc acctttatta
     4681 tataaatact attataactc tgcacttaaa tcttcatcag ttactgaaaa attatcttat
     4741 tataacagtg ctttaaaata ctctaaaaat gatgatttat taaattctat ttatacaact
     4801 ttaaagtctg actctgattt tgttgataat tcttcaatat taacaaactt aaataaatct
     4861 gaaaaagata atttaatgtc aaaattatat gtaaataaag ctactgtaga ttttaaaaat
     4921 aaaaattaca ccgactgtga ttctgattta gatctagcta caaaatatgg atataaaaaa
     4981 gaaaactttt cacaatatga tgatttacaa aaaaagctta atgaaagtaa aaattcttca
     5041 aataacgata aagtcgataa tgtctatagt tttacaaatg aaaatccatc aaaattttct
     5101 ggaaacatat atgattatcc ttatgacttt ataatgccat atagtaattc ttcctatttg
     5161 tcagcatcag atctttctaa atataataaa agtactcttg ctttaatgag aaacgaaata
     5221 tatgcacgtc atgggtatgt gtttaatact aatccattta aggaatattt taattcaaaa
     5281 tcatggtatc atcctgattc atcctttaaa ggtgatgaca gtgaattaaa tgattatgaa
     5341 ataaaaaatg tacaaaccat aaaatcagtt gaaaactcca aataaaggga tgatagtatg
     5401 aaaaattttt taagaatttt atctattata attatttcct ctttaatgct attaggttgt
     5461 ggatcaaatt ccactaaacc aaatattaat aaggactcta atagtattaa taattccaat
     5521 gaggaaaata acaagataga aaaaaataac tctaataata aaaataattc tatagatgaa
     5581 aataataata aagaaaaaaa tgattctaaa gaaggaaaca tagaccaaaa aaaagaaaaa
     5641 aataaaattg attctaatac tagtaaggaa actgataaaa aaagtgatgt agaaccttat
     5701 tgtgaaaatg aacctaataa agctaataaa gatataacta tagttattga tccagggcat
     5761 tctagtacaa tatctaatga aacagaacct gaatgtccag gctcacaaaa aagaaaatta
     5821 aaagatacct taggtgctat aggagttcaa agtaaaattc cagaatacac aataactcat
     5881 ggtgtagctg aagaattaaa aaaacttctt acttctgaag ggtataatgt aattatgact
     5941 aaagatagtc ctgacaagca gcttagcaat atagaaagaa ccactattgg taatgataat
     6001 aatgcaaatt taattataag gattcactgt gacggagttg attctcctaa ggcttgtggt
     6061 gcttctatac tagttcctgc tacaaaagga aatgtaacaa aagatatatc agatataagc
     6121 tattcatatg gagaaaaaat actaacagca tatacaaaat atacaggatt aaaaaataga
     6181 ggtgttgttg taagagatga tttaactggt tttaactggt caaaagttcc tattgtactt
     6241 atagaattag ggtttatttc caatcctaat gaagatttat atttaagtaa tccagataat
     6301 tatataaaaa ttgcaactgg aatctctaac ggaattaatt actgttttgc aaaataaaga
     6361 aaagggggaa aatatccccc ttttttttat ttttaataaa ttatttaata ggtaaattaa
     6421 gccatttcaa aactttctgt tgttttctct actatttcac ccttaacttt agaaactaaa
     6481 tctcccccct tagaagcaaa tatatttgct gtaactattt tttccataag attttgaact
     6541 tcttcctcag ttacatcttc cttaatattt ttaagagtta tttttgttat acttcctcca
     6601 gcatttttaa aagacaatac aagatataat tccttctcca ttttaaagtc cccctttttt
     6661 ctttaccatc aatttattat gttacatctt ttgttcttct actacttctt ctaaaacgca
     6721 tatcttgctc attaatctct tttgattctt tttgttagct tataatttat ctttaaaagt
     6781 tcctatctat aaattctaag gtttctctta gaaaaactaa taccctctct attcttttga
     6841 ttacctttaa tgtagaattt aaaagcattt ttattttttt aaatactaca aaatcagctg
     6901 atcacccctc taatttttat tagattattg agtgtcatca acaaacttaa agtcatcttc
     6961 ttttaataca gaattaactc ttgatgataa aagatctgct aatgcttttc ccacctcata
     7021 aagtttttca tcctctgcat caacctttat cttagaaaac ttttgtgttg aaaatttagg
     7081 gcttccacta tcatctaccc ccttttgata ctttaatact aagcttttct ttgttaagat
     7141 tgcctttgcc atacttatca actcctttca ctatataaat attccttact ctttatttgt
     7201 gttgtaaaat aataaaaaaa aactgtaata aaaacatttt tttgtttcta ttacagtttt
     7261 tctatattta taatactaag taaagttatt tgactatttt ttaattatca tcaggatctc
     7321 catatcttcc tgtctcagga ttatactctc ttccctgttc atcatgaagt ggaaccttct
     7381 tcccatcaac tattatataa tctatagttt gatcagaatc ctttccagcg tcttcagttt
     7441 cttgcatatg tccatatcca ccagtaacag gattatattc ttcacccttt gaattatact
     7501 taggaattac agtaccatcc tcaagtttta tagtttcctt acttttatcc tttgaattat
     7561 tactgcttcc actattattt ttatttgaat tattctttga tgaacttaca ccatttttcg
     7621 atttactatt ttgtgacgct ttatcacctt tagcattatt attttgagat tctttattac
     7681 ctttattatc attatttaaa gaactattgc tcttactttc ttgttctcct tctgaattag
     7741 aagcatcttg cttattgcta ttagccttat caatttcttt atccttagaa tcactttttt
     7801 gtgatttatc ttctgtatta tttttacttt cattattact agtgtcattg tcatcatcac
     7861 ttattgaagt tcctatattc tttaattgat ccttgtttat catattacca tctttatcat
     7921 atgtaactcc atttatagta acttcttcat cacccaagtg tgctacaaac caatttttta
     7981 tatcagtaca aactcctaaa attttattta agattctatc taaagttcca cattctttaa
     8041 gttcttgtcc attttctttt aaagtttcct gtatattatt cgaaaggcta tcaagttctc
     8101 cttttaattt agaataatct aatcctaaag aatttatatt ggctaaaaga ccaactaatt
     8161 cttgctcttg tgttggagtc aactcaatat tatagttatt tgtaatatta tttactattt
     8221 caccaacctc tttgtcattt ttaggtttat cctttataac ttcttcttta gcttgattta
     8281 ctatagcact tgctccatct cttccaatat cttgtccttc actattttta gccttagcta
     8341 aattatttgt tatacttaac tcttgccttg ctacttcctt attttcaaca ggtatatttt
     8401 ctccagtggc cttttcaaac gcttgaagaa ctcctgccaa agcagatgtc ccagttacag
     8461 ggaatggcgc tgtagcaata acatcagcat ctgtaacccc tgatgttaac aaagcattac
     8521 ttagcatagt tttggtaacc tctgttaaat tatttgtttt tacctttaat ccatatcctt
     8581 tttcttctat tttaacaaaa acacttgagt aagcattacc tttaaattct cctactattt
     8641 taggtaatcc aagttgctct cttatttcat taacattcat agttataacc ttaacatcat
     8701 taggtttaac tccaaattcc ttaaacatct cttgtttttg agttgaattt agattagctc
     8761 ctaaagttac tacttcctta gtatcggcaa aaactgttgt aaatgagcca atacttattg
     8821 ctccagctaa tataattgct gttattaatt tatttttttt catttgcctt cttcctttct
     8881 aagcattcat atcaaagctt aatcaaaaat atattaaatc ctattgctca atttaaaacc
     8941 ttcaatttct atctaaatta ccttttgaca gttcaaattt aaaataaaat attattctaa
     9001 aaatacaata aatttcaatc ttacttagat tatatcaaaa taacttttca aaagtttaca
     9061 aattatttta atttagcata aaccttatat ttttctataa tagaaattta atctatattt
     9121 tttaaaattt tgtagtataa ttgtaatgtg tattctttgt tgcgagatga tatataaaaa
     9181 tacttctttc agctgagaaa ccagtcagaa aaataatata aatatacttt acccctaatt
     9241 gtaaggaatt ctctgaacaa aaaggtaata aaaaattttt gaatcttaga aaggtttgat
     9301 gacaatatga gtactagaat tgctgttgtt ggcattgtta ttgaaagcct tgataatata
     9361 aaagaagtaa attctatatt aaataaatat gaaaaattaa ttgtcggaag aatggggtta
     9421 ccatacaaag aaaaaaacat cagagttata tctattgtag tagatggaac caccgatgaa
     9481 atttcagcta taacaggaaa aatcggtaaa cttaaaggag tttctgttaa agctgccata
     9541 tctaaaacta gcttttagct taattgcttt aagcttctta atcattgagt tcccataagt
     9601 atattttagt tatttatatt attttttgac ataccctaaa agtaatttta aaggagattt
     9661 gttatgttaa aagataacga aaaatacaat gctttagatt ttattaagga tgatgaaatt
     9721 aatagtctta tagcaaaagg aaaagaactt gtttatgaca aagaactagt tagagaaatc
     9781 attgaaaaaa gtaaaagtgc agaagggtta acacctgaag aaactgctgt tcttcttaat
     9841 cttgaagata aagaacttat tgaagaaatg tttaaagcag ctagacaagt taaggaaaaa
     9901 ttatatggaa aaagacttgt tgtatttgca cctttatacg taagtaacta ttgcgtaaat
     9961 aactgtactt actgtggata taaacactgt aatgatgaat taaaaagaaa gaaacttaac
    10021 aaagagcaac ttattgaaga agttaaagtt cttgaaagcc taggtcataa gagaatagca
    10081 cttgaagctg gagaagatcc agttaatgct cctctagatt atattttaga ttgtataaaa
    10141 tcaatatact caattaaatt tgataatggg tcaataagaa gaatcaatgt taatatagct
    10201 gcaacaacag ttgaagacta caagagactt aaagatgctg aaattggaac atatatttta
    10261 ttccaagaaa cttaccataa accaacttat gaaagattac atgtaagtgg tccaaaacat
    10321 aactataatt atcatacaac agctatgcat agagcaagag aggctggtat agatgatatt
    10381 ggtatgggag ttttatatgg actttacgat tacaagtatg aaacattagc aatgcttatg
    10441 cacgcaatgg atttagaaga aactacaggg gttggtcctc atacactatc agttccaaga
    10501 ataagaccag ctgaaaatgt tagcttagaa aattatcctt acttagttga tgatgaagac
    10561 ttcaaaaaaa ttgttgccat cttaagatta gcggtaccat atgcaggtct tatactttca
    10621 acaagagaag aaccaggatt aagagatgaa ataatagctc ttggagtttc tcaagtaagt
    10681 acaggttcat gtacaggggt tggtggttat tcagaatcat acgtagatcc tgaagaaaaa
    10741 ccacaatttg aagttgaaga tcatagatca ccagttgaaa tgatagaaag ccttatggaa
    10801 gctggatata taccaagtta ttgtacagct tgttatagag aaggtagaac tggcgaaaga
    10861 tttatggaaa tagttaaaag tggtgaactt tataaaatat gtgaagccaa tgctttaata
    10921 actttaaaag aatttattga tgattacggc acagataaaa caagagaaat cggagataaa
    10981 ttaattaaaa aatcaataga tgaaatagat aatgaatcct ttagaaaatc tgttgaagaa
    11041 aaaataaata agataagtaa gggaactaga gatttaagat tctagttaat taaatatatt
    11101 gtaacttttt atatcaaatt aattcgctag tcttaattaa atttaaatat tatttattta
    11161 aacctgtata aaatttataa ctttaatttc acattaaaat ttatatcttt catatgggta
    11221 aaaactaaca attaaaatca aaactaaaat taatttataa ttaatatata gccagcgtaa
    11281 gctggctttt tttgtgaaaa ttttagctac catcttttgg atcttcttat actactcttt
    11341 tttgtatagt ttttatatta gaaactttag gttaaactta tatatttata atttgattca
    11401 ccttaaggtt aaataaggaa tatactaaaa aaagtattaa atttgtagta actaggtaaa
    11461 cattatccag aatataaaaa actcataaat aatactgtta tattatctac aatattgtgc
    11521 atatcattct ttatcatatt catattgtta atttaaaaag tttaatgaaa attatttttt
    11581 ctttaaattt ctcttttttt cttatttttt caacctttgc ctaaatattt tcataataat
    11641 acactttaga atacattgtt aaaaatttga ctattaaatg taaaggttat agaatataat
    11701 taaacattat gtaaatatta ggaatgattt gagagtttaa cttattgtga gggaggtttt
    11761 tttatgacag tagatcaaaa ttcattagaa agaagaactt gttgtaatat acacaagata
    11821 gttttaactt tagagaaagg cttttcttct atggagtctt taaagtatgc agttataact
    11881 caccttcaaa atgaaggcta tagatgtaat gatacaaaaa acaatattgg agattatcca
    11941 ctattaaaaa taaatgctaa tgatttttta ttaactataa aagatataga tgaaaattct
    12001 ttaaacttcg aaggttgtag tgaggtagtt ttagtacctc atcactaaag ttaataattt
    12061 gattattatg gacaacttct ttacttaaga gttgtccttt ttagttttat attatttcat
    12121 taaaatcctc ttctaatctc atactcaaaa ttatataatt attcttgaat aattttattt
    12181 actaaaatat ataacaaatc acagatttta taatagcttt tggtaaaata ttaattattc
    12241 ttttattatt tcatactgtt tttaagctaa aatttctata tcttaagctt attttaaatt
    12301 aaatttaaag aaataaag
//
LOCUS       DAEVGI010000070        11910 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEVGI010000070 DAEVGI010000000
VERSION     DAEVGI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.070"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            339..1691
                     /locus_tag="K8M77_001563"
     CDS             339..1691
                     /locus_tag="K8M77_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBI7086058.1"
                     /translation="MNNEGNQFENFKYSQEIKELLNINLSNDDLNNLDHIYNYYDSQN
                     IQRDYEDLIEDIYIFIFKRNLSITQLSEIYKVNQRIIKRWLKELGMDKLLEVEKGAYF
                     VEKSNFKNNKQDELLEKNKLKFDFLEKSKHPKRVIEFLNYLENVKGKSLNTVKGYSVD
                     LGLFFKFLKVYKGLENNIELEKIEEVEISDLGDNFIKDITLSDIYAFLAFLEKVRNNS
                     AYARARKVATLKSFFKFLNSKIKLIDENPTVELESPKINKRHPVYLTLDQSITVLNSM
                     DKSNKNYYRDYCILTLFLNCGMRLSELCNIEIEKIKGDTLTIIGKGNKERTVYLNEAS
                     IAAIENYLKNRNDSKATEEAKKYLFLSSKYRPINKRSVEILVKKHIENAGFKDQKYTP
                     HKLRHTAATLMYKYGNVDIRSLQNILGHENISTTQIYTHVDDETLRKAVKTNPLANIK
                     "
     gene            complement(1782..2174)
                     /locus_tag="K8M77_001564"
     CDS             complement(1782..2174)
                     /locus_tag="K8M77_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086059.1"
                     /translation="MDKKVFNNDDYCDVYNGKICDNCGDCLEMQGIDTKAIRIADIAK
                     EVEENKKIEETLKKLAEEEQEEMEDDLEEELTTETWEEYLAKQDEYEDAFNHIEYLED
                     IDLNDDLTMEEMTEEVFPGVRKLKIKEK"
     gene            2383..2994
                     /gene="lexA"
                     /locus_tag="K8M77_001565"
     CDS             2383..2994
                     /gene="lexA"
                     /locus_tag="K8M77_001565"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBI7086060.1"
                     /translation="MIIKENSDKQTQIYNFLIEFTKSKGYPPSVREICQAVSLKSTST
                     VHGHLKRLEKKGLIYRDPTKPRALEIVELSNEEKELIDIPIVGKVTAGMPILATENIE
                     DMFQIPINYVKHNNDLFILKVTGDSMIEAGILDGDLAIIEQKNIATNGDIVVALIENE
                     ATIKRFFKENGFIRLQPENKNYEPIIVEDCSILGKLVGIYRAY"
     gene            complement(3182..4087)
                     /locus_tag="K8M77_001566"
     CDS             complement(3182..4087)
                     /locus_tag="K8M77_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086061.1"
                     /translation="MEESRNKELKVKSFRVTEETFDKFKKIASDEFGNQGQCLDALIS
                     LYELENSKSTLIERKLEIESFQDYLNKINQLFLTSLQMSEDAGKRAEEEFFKKLSIKD
                     VTIERLQRREEELIERDRTLKEDNKAKTKEIEELKENIKTLEKDKSTLSQLVSRNYDL
                     IEKNKEEIASLKSLESLKGENEELRNKREEDRASLKERESHIKSLELEKESLKEKLNF
                     YEEKEKSYKEEVESYKKLVEAMRKDHKKELELLEVKYSKMAEKESEKLKKDFESRLEL
                     EKRTLELDIKTLKYEKEVLESKLNS"
     gene            complement(4346..5626)
                     /locus_tag="K8M77_001567"
     CDS             complement(4346..5626)
                     /locus_tag="K8M77_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBI7086062.1"
                     /translation="MLNATIELLKKQYGFTDETIKLYNQAIIDVENEFKIYDEIREYN
                     QLKVLKAFQEERISDNHFTNSTGYGYGDIGRDTLDLVYARIFNAEKALVRPHFVNGTH
                     ALGTALFGNLRPGDTILAVTGSPYDTLHSVIGISGEENIGSLKEYGVNYKQVDLVDGK
                     INIEKALEVIKEDESIKLIHMQRSTGYGWRNAFQVKELGEAIKAIKELREDLIVFVDN
                     CYGEFIDIIEPTDVGADLIAGSLIKNIGGGIAPTGGYIVGKEKYVEQASYRLTVPGIG
                     AECGCTFGVMKDFYQGLFLAPHVAIEALKGAIFCARIMELAGFEVLPKYNDKRSDIIQ
                     AIKFNDKEKLIKFCKGVQYASPIDSFVECEPWAMPGYSDEVIMAAGAFIQGSSIELSA
                     DAPIREPYIAYLQGGLTFDHAKIGVLIALNNIFK"
     gene            complement(5728..5970)
                     /gene="hfq"
                     /locus_tag="K8M77_001568"
     CDS             complement(5728..5970)
                     /gene="hfq"
                     /locus_tag="K8M77_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBI7086063.1"
                     /translation="MNKSINNLQDIFLNNARKERIPVTIFLVNGVQLKGIVKGFDSFT
                     VVLDSDGKQQLVYKHAISTVSPAKPILFNSAQVFDN"
     gene            complement(6014..6946)
                     /gene="miaA"
                     /locus_tag="K8M77_001569"
     CDS             complement(6014..6946)
                     /gene="miaA"
                     /locus_tag="K8M77_001569"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBI7086064.1"
                     /translation="MNNNLLIIAGPTAVGKSDLSVDLAKKLNGEIISVDSMQIYKYMD
                     IGSAKISKEEMGGIPHYLIDFVDPSKEFSVAEFKELATEKIKDIQSRGKLPILVGGTG
                     LYINSIICNMNFAESDKDEEYREELEKIANEHGNEYLHEMLKDIDLESYNSIHFNNRK
                     RVIRALETYKLTGKPFSSFKAKNSIYETPYNIYYYVLNMDRAKLYERINKRVDIMFEK
                     GLLEEVKNLKAMGLTDDMQSMKGIGYKEVLYYLDGKISLEQCIEMIKQGSRNYAKRQL
                     TWFRKDPRAIFIDKDTFSSEEDISSKIINDIINS"
     gene            complement(6964..8988)
                     /gene="mutL"
                     /locus_tag="K8M77_001570"
     CDS             complement(6964..8988)
                     /gene="mutL"
                     /locus_tag="K8M77_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBI7086065.1"
                     /translation="MNRINILNADTANKIAAGEVVERPSSVVKELVENSLDAGAKNIT
                     IEIQNGGESLIKIIDDGSGVHPEDVEKAFNPHATSKIKDTYDIFSINTLGFRGEALPS
                     IASIARVDFKSKTEDFDMGKELIISGGEKESLTDCSMNRGTQIEVRNLFFNVPARKKF
                     LKTTARESALINDLVNRISLANPDVSFKLFNNNKKILNTYGNGKLIDVIRTIYGKSTA
                     ENLIYFEEHKDTASVYGFIGNDTLARASRNNQSLFVNKRYVKNRSLTVAVENAFRSFN
                     VTGKFPFFVLFIDTYPELIDVNIHPTKSEIKFKDERFIFKLVFDAVHSAMREYVKDTF
                     TLPEEEEKKFEALKEEVIQESLDKEISTLEKLKENINYKVSDDKRKEEIYSYNPSKDY
                     EAKTEVNIPVDFLSKENQEESSKFNNSLENNDFKEVSAKREISYEPILIKNELKDKVS
                     ENTYDSLESSDYKCNKNEYGNSLEERIYREAKFPKLKVIGQFNKTYILAEYDSTLYLI
                     DQHAAHEKILFEKYSSDIAKKKVEIQPLMIPLVVTLPTEDYLYYDENKEIFEKAGFKI
                     SDFGDNSIRIEEVPYFLDKLNPTELIISMINNLKKMGTGETVEVKYNKIASMSCRAAV
                     KANDVLSILEMENLIEDLRYINDPFHCPHGRPTIIKFTSYELDKKFKRIT"
     gene            complement(9015..11747)
                     /gene="mutS"
                     /locus_tag="K8M77_001571"
     CDS             complement(9015..11747)
                     /gene="mutS"
                     /locus_tag="K8M77_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI7086066.1"
                     /translation="MKLTPMMRQYFEIKENYKDCILFFRLGDFYEMFFEDAETAAREL
                     ELVLTGRDCGLEKRAPMCGIPFHASNSYIGRLVAKGYKVAICEQVEDPKVAKGIVKRD
                     VIKVITPGTYTDSSFVEETKNNYIMTIYSDLERNRCSLAITDISTGDFLATEGELEKG
                     VILDEISKFNPKEIILLDSLDQELIKDITLTTPALISRKPIEYFEEKFEEVLNSQFGE
                     KSNSLSLMIKKSSNALVKYILDTQKISLTNINDIEVYSLVDFMTIDLSSRRNLELTEN
                     LREKSKKGSLLWVLDKTETSMGSRMLRRWIEEPLVNKEKITLRLNAVEELFNDLSLND
                     SLKEALHDIYDIERILGKISNKNANAKDLIALKTSIGKIPNVKGIIENCTSSLLKNYH
                     HNLDDLRDIYELLEKSIKEDPSLTLKDGDLIKDGFNGEIDELRLAKTNGKDWISSLEN
                     REREFTGIKSLKVGFNKVFGYYIEISKANYSSIPEGRYIRKQTLANAERFITPELKEI
                     EEKLLGASEKLCSLEYDIFLDIRNEVENHIDRLKTTAKIIAELDCISNLAFVALENDF
                     IKPEINEDGETKIENGRHPVVEKVIPKGEFIPNDTIINKDDNQLLIITGPNMAGKSTY
                     MRQVAIITLMCQIGSFVPASKANISVVDKIFTRIGASDDLAGGKSTFMVEMWEVSNIL
                     KNATENSLVLLDEVGRGTSTYDGLSIAWSVIEYICKNKNLRCKTLFATHYHELTKLEG
                     EIHGVRNYSVAVKEVDNNIIFLRKIIEGGADQSYGIEVAKLAGIPDEVINRAKEILET
                     LEMESSKDNLDLALKEVNASKEEMKEASITSSYEVKETIVEEDKIEIKEEIISKSSDA
                     KTHKKEEDQIQLDFSAIGKDNLIKELSEVDILSLNPMEAMNRLYALVKEAKNLI"
BASE COUNT         3998 a         1990 c         1304 g         4618 t
ORIGIN      
        1 ttatatttaa gatgtattgg catttattat tttgtataaa acgtattact ttcaatcgta
       61 atactaattg tattacaatt tattataatg agtgctttat aaattttagc taaaagatat
      121 ttttaatgaa atgagatatt tcaaagttaa attaaaatat ataggaagta actaaaatta
      181 aaaaacaatc ttaaatcaga ttgtttgaaa tacatatatg aatttctaaa atagctcaat
      241 ttataacttc gctaaatata catttagcga agttattgtg ctttttgata aaatatgcta
      301 caatatagtc atatcaaggc tttgaaagga tttagactat gaataatgaa ggaaatcaat
      361 tcgaaaattt taaatactca caagaaatca aagaattact gaatataaat ttaagtaatg
      421 atgacttaaa caatcttgat catatataca actattacga ttctcaaaat atccagcgtg
      481 actatgaaga tttaattgaa gatatttata tttttatttt caagagaaat ctaagtatta
      541 cacaactgtc agagatctat aaagttaatc agagaataat taaacgatgg ttaaaggaac
      601 ttggaatgga caagcttttg gaagttgaaa aaggcgctta ttttgttgaa aaatcaaatt
      661 ttaaaaacaa caaacaagat gaattgctcg aaaagaataa attaaaattt gattttttag
      721 agaaatcgaa gcatccaaaa agagttattg aattcttaaa ctacttagaa aatgtaaaag
      781 gaaaatcact aaacaccgta aaaggatatt cagttgattt aggattattt tttaaatttt
      841 taaaagttta taaaggtcta gaaaataata ttgaattaga aaaaattgaa gaagttgaaa
      901 tttctgattt aggagataat tttataaagg atataacact atctgatata tatgcatttt
      961 tagctttttt agaaaaagtt agaaacaata gtgcttatgc cagagctaga aaagttgcta
     1021 ctttaaaatc tttctttaag tttttaaatt ctaaaataaa actaatagat gaaaacccta
     1081 cagtagaatt agaatctcca aagataaata aaagacaccc agtttattta accttagatc
     1141 aaagcataac tgttttaaac tctatggata aatcaaataa aaattattat agagattatt
     1201 gcatactaac tttattttta aactgcggaa tgagactatc tgaactctgt aatattgaaa
     1261 tagaaaaaat caaaggagat accttaacta ttataggtaa aggaaataaa gaaagaactg
     1321 tttatttaaa tgaagcttca attgcagcta tagaaaacta tttgaaaaat agaaatgatt
     1381 caaaagctac tgaagaagct aaaaaatact tatttttatc aagtaaatat aggcctataa
     1441 acaaaagaag tgttgaaatt ttagtgaaaa aacatataga aaatgcaggg tttaaagatc
     1501 aaaaatatac accacataaa cttagacaca cagccgccac ccttatgtat aaatacggta
     1561 atgtagatat aagaagcttg caaaatatat taggtcacga aaatatttca acaactcaaa
     1621 tatataccca tgtagatgac gaaactttaa gaaaagcagt taaaactaat cctttagcaa
     1681 atataaaata gcatcacttc ttgattttat gaagaaaata cgaggataga acatatgttc
     1741 tatcctcgta tttttatcac ttttatataa gaaacactat tctacttttc ttttatttta
     1801 agttttctaa cccctggaaa tacttcttct gtcatttctt ccatagttaa atcatcattt
     1861 aaatctatat cttcaagata ttcaatatga ttaaaagcat cctcatattc atcttgctta
     1921 gctaaatact cttcccaagt ttctgttgta agctcttctt ctaaatcatc ttccatttct
     1981 tcttgttctt cttcagcaag ttttttgaga gtttcttcta ttttcttgtt ttcttcaact
     2041 tcttttgcta tgtcagctat tcttatggct tttgtatcta tcccttgcat ctctagacaa
     2101 tcaccgcaat tatcacaaat ctttccatta tatacatcac aataatcatc attgttaaat
     2161 acttttttat ccataaagtc acctcttact taaaaatcat cttttcatta taacaaatat
     2221 actctttttt atcaaataaa aagttgtttt tccataaaaa aataatacaa aaaccacctt
     2281 attttaagtg tttttatgaa agctacggta tgagaacata agtttgctat actatcctta
     2341 ctgtggtata atattaccaa agaatcgtga ggtgcaatta ctatgattat taaggaaaat
     2401 tcagataaac aaactcaaat atataacttt ttaattgaat ttacaaaatc aaaggggtat
     2461 ccaccttcag ttagagagat atgtcaagca gttagcttga aatcaacctc aactgttcat
     2521 ggacatttaa agagattaga gaaaaaagga ttaatatata gagatccaac taagccaaga
     2581 gctttagaaa tagtagaatt atctaatgaa gaaaaggaat taatagatat tcctatagta
     2641 ggtaaagtta ctgctggtat gcctatttta gctacagaga atatagaaga catgtttcaa
     2701 ataccaataa actatgttaa gcacaataat gatttattta ttttaaaagt tactggagac
     2761 agtatgatag aagctggaat cttagatgga gatttagcaa taatagaaca gaaaaacata
     2821 gcaacaaatg gtgatattgt agtagctcta atagaaaatg aagctaccat aaaaagattt
     2881 tttaaggaaa atggatttat aagattacaa ccggaaaaca aaaactatga acctataata
     2941 gttgaagatt gctctatttt aggtaaatta gtaggaattt atagagctta ttaagaaaaa
     3001 atgctaggga attttatgcg ttacaaattt cctagcgttt tttcttattt aacaatataa
     3061 aaacttatta ttttcttatt atttactaaa tattaagcta taatctatac ggaatattaa
     3121 attacacaaa ttattctaca ttctcaatcc attaataact caatttacaa atcaacatat
     3181 attatgaatt caacttactt tctaaaactt ctttttcata ttttaaagtt tttatatcta
     3241 attctaaggt tcttttctct aattctaacc tactttcaaa atccttttta agcttctcag
     3301 actctttttc tgccatttta gagtatttaa cctctaataa ctctaactcc ttcttatgat
     3361 cctttctcat agcttctaca agctttttgt atgattcaac ctcttcctta taagacttct
     3421 ctttttcctc gtagaaattt agtttttctt taagagattc cttttctaat tctagagatt
     3481 ttatatgaga ttctctttct tttaaagaag ctctatcctc ttctctctta tttcttaact
     3541 cttcattttc accttttaaa gattctaaag attttaaaga agctatttct tctttatttt
     3601 tttctatcaa atcatagttt ctagatacta attgagataa ggtgctttta tctttttcta
     3661 aagtttttat attttcttta agctcttcta tttcctttgt tttagcttta ttatcctctt
     3721 ttaaagtcct atctctttct ataagctcct cttctcttct ttgaagcctc tctatggtca
     3781 catcctttat agaaagcttc ttaaaaaatt cctcttctgc tctttttcct gcatcctcac
     3841 tcatttgaag agaagttaag aaaagttggt ttatcttgtt taaataatct tgaaaagact
     3901 ctatttctaa ctttctttct atcaatgttg atttagaatt ctctaattca tacagtgata
     3961 ttaaggcatc taaacattgt ccttgatttc caaactcatc agatgctatt ttcttgaatt
     4021 tatcaaaagt ttcttcagtt accctaaagc tttttacttt taattctttg tttctgctct
     4081 cttccaaaat atccttccac ctttctttgt aatacaatat gtaatatata aaatgtaata
     4141 cgtaatatta aattgtttaa ctattttaat tatatcttat aaaaaagatt tattccataa
     4201 aaagaatata aaaaataggc ctttatcaaa ataaatttca ataagattta aagtaaatag
     4261 tttatgagaa atatctttgt aaatcttatt gttttttgat aaaagcctat gttaattttt
     4321 gtatttacta atttaaatta gcatattatt taaaaatgtt atttaaagct attaatacac
     4381 ctattttagc atggtcaaaa gttaatccac cttgtaaata agctatatat ggctctctta
     4441 taggtgcatc tgctgaaagt tctatagaag aaccttgaat gaaggcacca gctgccataa
     4501 taacctcatc tgaatatcct ggcatagccc atggttcaca ttcaacgaaa gaatctattg
     4561 gtgaagcata ttgaacaccc ttacagaatt ttataagttt ttctttatca ttaaatttta
     4621 tagcctgaat tatgtcactt ctcttgtcat tatatttagg aagaacttca aatccagcaa
     4681 gctccattat tctagcacag aatattgctc cttttaaggc ttctatagca acatgaggag
     4741 ctaagaataa tccttggtag aaatctttca tcactccaaa tgtacatcca cattctgctc
     4801 ctataccagg tacagtaagt ctataagatg cttgctctac atatttttct tttcctacta
     4861 tgtatccacc agttggagct attcctccac ctatgttttt aattaatgat cctgctatta
     4921 aatcagcacc aacatctgta ggttctatta tgtctataaa ctctccataa caattatcaa
     4981 caaatactat taaatcttct cttaattctt ttatagcttt tattgcttct cctaattctt
     5041 taacttggaa agcatttctc cagccgtatc cagttgatct ttgcatatgt attaatttta
     5101 ttgattcatc ttctttaatc acttctaaag ctttttctat attaatcttt ccatctacaa
     5161 gatctacttg cttataatta acaccgtact cttttaatga tcctatattt tcttctccac
     5221 ttataccaat tacactatgc aaagtatcat aaggactacc agtaacagct aatatagtat
     5281 ctccaggtct taagtttcca aataaagctg ttcctaaagc atgagttcca ttaacaaaat
     5341 gtggtctaac taaagctttt tcagcattaa agattctagc ataaactaaa tctaaagtat
     5401 ctcttccaat atcaccataa ccatatcctg ttgaattagt gaaatgatta tctgaaattc
     5461 tttcctcttg gaaagcctta agaactttta attgattata ttctcttatt tcatcatata
     5521 ttttaaattc attttctaca tcaattatag cttgattata aagctttata gtttcatctg
     5581 tgaaaccata ttgttttttt agtaattcta tagttgcatt aagcattatt atacccccgc
     5641 attattaaaa tatcgtacat ttataaatct tatcatatag aaaaaaaata ggcaaaccat
     5701 ctagggttta cctatttttt tctatattta attatcaaaa acttgcgcac tattaaataa
     5761 aataggttta gctggtgata ctgtgcttat agcatgttta taaactaatt gttgctttcc
     5821 atcactatct aaaactactg taaagctatc gaacccttta acgataccct ttaattgaac
     5881 cccatttact aaaaatattg ttacaggaat tctttccttt cttgcattgt ttaagaatat
     5941 atcttgtagg ttattgattg acttattcat tctattgccc tccataaatt tttaatataa
     6001 tttaattata aacctaagaa tttattatgt cattaataat tttagaagaa atatcttctt
     6061 cagaggagaa agtatcttta tctataaata tagctctagg atcctttcta aaccaagtaa
     6121 gctgacgttt agcatagttt ctgctccctt gcttaatcat ctctatgcat tgttctaaag
     6181 agattttgcc atcaagataa tataaaactt ctttgtatcc tatacctttc attgattgca
     6241 tatcatcagt taatcccata gctttaagat tcttaacctc ttctaaaaga cctttttcaa
     6301 acattatatc aactcttttg tttattcttt cataaagctt tgctctatcc atattaagta
     6361 cataataata tatattatat ggagtttcat aaatactatt cttagcttta aaagatgaaa
     6421 atggttttcc agttaactta taagtttcta atgccctaat tacccttttt ctattattaa
     6481 aatgtattga attatagctt tctaaatcaa tatcttttag catttcatgt aaatattcat
     6541 ttccatgctc attagctatt ttttcaagct cttctctata ctcttcatcc ttatcacttt
     6601 ctgcaaaatt catattgcat atgattgaat ttatatatag tccagttcct cctactaata
     6661 taggaagttt ccctctactt tgtatatctt taattttctc tgtagctagt tctttaaatt
     6721 cagctacact aaattcctta ctaggatcaa caaaatctat taaatagtga ggaatacctc
     6781 ccatttcttc cttagaaatt ttagctgaac ctatatccat gtatttatat atttgcatag
     6841 agtcaacaga aattatttca ccatttagct tctttgctag atctactgaa aggtcacttt
     6901 ttcctacggc tgttggccca gcaataataa gtaaattatt attcatttaa actgtatctc
     6961 tccttaagtt attcttttaa acttcttatc taattcataa ctagtaaatt taattatggt
     7021 tggacgtcca tgtggacagt gaaaaggatc atttatatat cttaaatctt ctattaagtt
     7081 ttccatttct agtatgctta aaacatcatt agccttaact gccgccctac aggacataga
     7141 tgctatttta ttatatttaa cctctacagt ttctccagta cccattttct ttaagttatt
     7201 tatcatagat attattagct ctgttggatt taatttatct aaaaagtatg gtacctcttc
     7261 aattcttata gaattatcac caaaatcact tattttaaat cctgcctttt caaaaatctc
     7321 tttattttca tcgtaataaa gataatcctc tgtaggcaat gttactacta gtggaatcat
     7381 tagaggttga atttcaacct tctttttagc tatatctgaa gaatactttt caaataaaat
     7441 cttctcatga gctgcatgtt ggtctattaa atataaagta gaatcatact cagctaatat
     7501 ataagtttta ttaaattgac caataacttt tagcttaggg aattttgctt ccctataaat
     7561 tctttcctct agggaatttc catattcatt cttattacat ttataatcac ttgattcaag
     7621 tgaatcataa gtattttcac ttactttatc tttaagttca ttttttataa gtataggctc
     7681 ataagaaatc tctcttttag ctgaaacctc tttaaaatca ttattttcta aagagttatt
     7741 aaacttagaa gattcctcct gattttcttt tgataagaaa tctactggaa tattaacctc
     7801 tgttttagct tcataatcct tagaaggatt ataagaataa atctcttctt ttcttttatc
     7861 atcgcttacc ttataattta tattttcttt taacttttct aaggtactta tttccttatc
     7921 taagctttcc tgaataactt cttcttttaa agcttcaaat tttttctcct cttcttctgg
     7981 aagagtaaag gtgtctttta catattccct catagctgaa tgaacagcat caaagactaa
     8041 cttgaatata aaacgttcat ctttaaattt aatttcagat tttgttggat gtatgttaac
     8101 atctataagc tctggataag tatctataaa taatacgaag aatggaaact tacctgtaac
     8161 gttaaaggat ctaaaggcat tttccacagc tacagttaag cttctatttt ttacatatct
     8221 cttatttaca aaaagacttt gattatttct agatgctctt gctaaggtat catttcctat
     8281 aaatccataa acagaagctg tatccttatg ctcttcaaaa tatattaaat tttcagcagt
     8341 ggacttacca taaatagttc ttataacatc tattaatttt ccattgccat aagtatttaa
     8401 aatcttttta ttattattaa ataatttaaa tgatacatct gggttagcta gtgaaattct
     8461 atttactaag tcatttatta aggcactttc tctagccgtt gtctttaaaa acttctttct
     8521 agcaggtaca ttaaagaata aattcctaac ttctatttga gttcctctat tcatagaaca
     8581 atctgttaaa gattcttttt ctcctccgct aattattagc tctttaccca tgtcaaagtc
     8641 ttctgtttta cttttaaaat caacccttgc aatagaagct atactaggca aagcttctcc
     8701 tctaaatcca agagtattta tactaaaaat atcataagta tcttttattt tacttgtagc
     8761 atgaggatta aaagcttttt caacatcctc tggatgaact cctgagccat catctattat
     8821 ttttataaga gattctccac cattttgaat ctctatagtt atattttttg cccctgcatc
     8881 taatgaattt tctacaagtt ctttaaccac agaagaaggt ctttcaacaa cctctcctgc
     8941 tgctatttta tttgctgtat ctgcatttaa aatatttatt ctattcaaaa tttataacag
     9001 ctccttccca taagctaaat taaattttta gcttctttaa ctagagcata taatctattc
     9061 atagcctcca tagggtttaa agataaaata tcaacttctg aaagttcttt tatcaaatta
     9121 tcttttccta tggcagaaaa atctaattgt atttgatcct cttctttttt atgtgtttta
     9181 gcatccgaag attttgaaat aatttcttct ttaatttcaa ttttatcctc ttctactatg
     9241 gtttctttaa cttcataaga ggatgtaata gaggcttcct tcatttcttc ttttgaagca
     9301 tttacttctt taagagctaa atctaagtta tcctttgaag attccatttc aagagtttct
     9361 aagatttcct tagctctatt tattacctca tctgggatac cagcaagttt agcaacctca
     9421 ataccataag attgatctgc tccaccttct attatttttc ttaagaagat aatattgtta
     9481 tcaacttcct tcacagctac tgaatagttt ctaactccgt gaatttctcc ttctaattta
     9541 gttaactcat gataatgagt agcaaaaaga gttttacatc ttaaattttt attcttacat
     9601 atatactcta taacagacca agctatactt aatccatcat atgtactcgt tcctcttcca
     9661 acttcatcta aaagaaccaa gctattttct gtagcatttt ttaaaatgtt agaaacttcc
     9721 cacatttcaa ccataaatgt acttttacca cctgctaaat catcagaagc tcctattcta
     9781 gtaaagattt tatctactac acttatatta gccttactag ctggtacaaa agaacctatt
     9841 tgacacatta aagtaataat agctacctgt ctcatatatg ttgatttacc agccatatta
     9901 ggacctgtta ttataagaag ttgattatca tctttattta taatggtgtc attaggaata
     9961 aactctccct taggaattac cttttcaaca acagggtgtc ttccattttc tattttagtt
    10021 tccccatctt cattaatttc aggttttata aaatcatttt ctaaagctac aaaggctaaa
    10081 ttgcttatac aatctagttc agcaattatc ttagcagtag tctttaacct atctatatga
    10141 ttttcaactt catttcttat atctaggaaa atatcatatt ctaaagaaca aagtttttca
    10201 ctagctccta aaagtttttc ttctatttcc ttaagttctg gagtaataaa tctctctgca
    10261 ttagctaaag tttgctttct tatatatctt ccttcaggaa tagaactata attagcctta
    10321 gatatttcta tataataacc aaaaacctta ttaaatccta cctttaaaga ttttatgcca
    10381 gtaaactctc tttctctatt ttctaaacta gatatccaat ccttaccgtt agtcttagca
    10441 agtcttaatt catctatttc accattaaat ccatccttta ttaaatcacc gtcttttaat
    10501 gttagagagg gatcttcttt tatagatttt tctaaaagct cataaatatc tcttaaatca
    10561 tctaaattat ggtgataatt ttttagaaga cttgaagtac aattttctat aattcctttt
    10621 acattaggaa ttttacctat agaagttttt aatgctatta aatccttagc attagcattc
    10681 ttgtttgaaa ttttacctaa tattctctct atatcataaa tatcatgtaa agcttcttta
    10741 agagagtcgt ttaaagaaag atcattaaat aattcttcaa cagcatttaa tcttaaagtt
    10801 atcttttcct tattaacaag aggttcttca atccatcttc taagcattct acttcccata
    10861 gaagtttctg tcttatctaa tacccaaaga agagaacctt tcttgctttt ttctcttaaa
    10921 ttctctgtaa gctctaaatt tcttctagaa cttaaatcta tagtcataaa atcaaccaaa
    10981 ctatatactt ctatatcatt tatattagtt aagcttattt tttgagtatc tagtatgtat
    11041 ttaactaaag cattacttga tttcttaatc attaaactta gagagtttga tttttctcca
    11101 aattgagaat ttaatacttc ctcaaatttt tcttcaaaat attctatagg ctttctactt
    11161 attaaggctg gagtagttaa tgttatatct tttataagct cttggtctaa agaatctaaa
    11221 agtattattt ctttaggatt aaacttagat atttcatcta aaataactcc cttttctaac
    11281 tctccctctg tagctaagaa atctccagta gaaatatctg ttatagctag tgaacatcta
    11341 tttctctcta aatctgaata tatagtcatt atataattgt tctttgtttc ctcaacaaaa
    11401 gaagaatctg tatatgttcc aggagttata acctttataa catccctttt aacaattcct
    11461 ttagcaactt taggatcttc aacttgttca caaatagcaa ccttatatcc tttagctact
    11521 aatcttccta tatatgaatt agatgcatga aacggaattc cacacatagg tgctcttttt
    11581 tctaagccac aatctcttcc agtaagaact aattctaatt cccttgcagc tgtttctgca
    11641 tcctcaaaga acatctcata gaagtctcca agtctaaaaa atagaataca atccttataa
    11701 ttttccttta tctcaaaata ttgcctcatc atcggagtca gcttcatttt agtacctcca
    11761 tcatttctat atgctaaaac cttataacat tttctaaaaa tatcataatg aataactatt
    11821 ataactttta aatataaatt ctttttattt aattactaaa gcctaaaaac ttttctttaa
    11881 aaagttaata aaatatactt tgattttttt
//
LOCUS       DAEVGI010000071        11851 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEVGI010000071 DAEVGI010000000
VERSION     DAEVGI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.071"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..353)
                     /locus_tag="K8M77_001572"
     CDS             complement(<1..353)
                     /locus_tag="K8M77_001572"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI7086067.1"
                     /translation="MCSILNVPRSTYYSKKNPKKSEREIENEKLKTAILKYYKESKCR
                     YGAPNIKIMLSKNGFNISIKRTQRLLRELGIKSIIIKKYRPTSKKKPKEGLENLLKRD
                     FTTTSINEKWVGDIT"
     gene            complement(460..699)
                     /locus_tag="K8M77_001573"
     CDS             complement(460..699)
                     /locus_tag="K8M77_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7086068.1"
                     /translation="MGRGKKYTEDYKEMISDLYKSGMTISEISSEYGIAKSTINGWVK
                     ANKEIKISEDEVITLKEVAKLKKEMARIKEENEIL"
     gene            753..1244
                     /locus_tag="K8M77_001574"
     CDS             753..1244
                     /locus_tag="K8M77_001574"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086069.1"
                     /translation="MCPLFYTNTNIDEVPNYRIENNARVEAYRVEPGDLIISSRGATK
                     VAIIPENSENLLISQNFIGLRLNNENDPEYIKELLLSPIGRYFIDRIKTGTVVQVINQ
                     KDFDEMPLVCFNHEKQKKIMKEYKEEEKELNKKIEELKRKLENMKLNLYEKMKIKDGF
                     EIL"
     gene            1372..1944
                     /locus_tag="K8M77_001575"
     CDS             1372..1944
                     /locus_tag="K8M77_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086070.1"
                     /translation="MRVLFCNVAWMKYYDGISEDDKPINGGKFIYENEDGAEIYNFTL
                     YSDDNFHGFVSTKSNRGRQNQLHIERFEGVSEDDEEADNVLVIWVAKDPARGKSYIVG
                     WYKNATVFRNYYYDEDLCPKNIYANAKDCVLLPMNKRQKIVPRAGRDGYLYGFGRANV
                     WFGKKGDEKANEYIKNMVEYINTYDGESLI"
     gene            2027..3517
                     /locus_tag="K8M77_001576"
     CDS             2027..3517
                     /locus_tag="K8M77_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HBI7086071.1"
                     /translation="MDKLYVDFINEITKEELYEGLLAYGLFSDKLPPFLSSKGFYDYC
                     KNLNHEFKAGEKQYIYYESIRNINFPRPLGIPNPMAYQKLCKCLSEKWDKIQEHFKKM
                     TENQLYKVSRIHIRKLNNSRKLFEMSYNNWMSDGTPELDLLIGKRYMVKADISNCFPS
                     IYTHSLPWALVGKETAKKNRNGKKWFNKIDRCAQNIKNGETHGLIIGPHTSNLLSEII
                     LCFIDNKLNKWDYIRNIDDFTCYVETYDKAQEFLVELSKELREFDLVLNHKKTEIIEL
                     PIESKELWINKLNSASLLLKGRKVNYNEARLYLDLTIDLFKCNKMNSAILNYAIKVLG
                     SFELTYNAKQYCLKTIFHWTLIYPYLVIILEKNVFNVFSADISQINEISQRIFKNGLE
                     NRNYEAVSYAIYFAIKYKFELSDICAYDAIKSDHCIYKLLTYIYFAKNNDNKSKELLE
                     DHAKNLKSNDEDFNRNWLFVYEVLPVDCFEDEWKAIKRNNVSFINI"
     gene            3656..6412
                     /locus_tag="K8M77_001577"
     CDS             3656..6412
                     /locus_tag="K8M77_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBI7086072.1"
                     /translation="MTYQSEHELEELLINQLVEQGYAIVNIPDEAALEKNFRETLFEF
                     NRKKLGGAAFTDKEFERILIHLKNKSIFNSAKIFRDKFILERENGTKVYIEFFDGKDY
                     HNNIFQVSNQITMEGKYVNRYDVTILINGLPMVQIELKRRGKDFYEAFDQIERYRRHS
                     LKGLFRYIQCFVISNGVDTKYYSNSDKEIKREFTFFWSDEKNKRVTNLREFAESFLDR
                     NMLNKIIARYMIINESEKNLMVMRPYQIYAVEALMKRANETNNNGFIWHTTGSGKTLT
                     SFKASQLLADEDKVKKVFFLIDRKDLDSQTVEEFNKFEKDCVDTTDNVRTLIKQIEDV
                     NTRLIVTTIQKLARLLKSEKYAHRMDKYRDEKVIFIIDECHRSQFGEMHTAINKHFTK
                     AQYFGFTGTPRLPENRSQDGRTTADIFDKCLHHYLIKDAINDGNVLGFSVEYIKTFDG
                     QVNEYDDEYIKAIDKEEVFMNDERIENIAKDIIANHDKKTAGRKFCSLFTVQKIPMLV
                     KYYDAFKALEHDLKIAAIFTYDDNENLDGKCEHSRESLDGIISDYNKMFKEEDIDFST
                     DTFKEYSKHLSKKIKNAEVDIVIVVNMFLTGFDSKPLNTLYVDKNLKYHGLIQAYSRT
                     NRILDGTKQHGNIVCYRNLKKETDEAICLFSQTDSTDVVLMESFDHYLKLFQERLDRL
                     YETAPTIESIDSMQDENEKKDFVEAFRELTKQLTKLKTFVEFEFNEKKLGISAQKYED
                     YKSKYFAIYESVKKDGKTSVLVDIDFCIELMQTDKINVNYILNLIRTIDFETEEKTKK
                     DIEKIKRLLDNADNENLRLKSELLREFLDQVVPNITKNDSVDEAFNEFLEDKRVDEIE
                     YFSNEVEIDKFKVKEYVSEYEYSGRIDGSEIKDNLSGGLLKKKRLAEKIKTFIINHVT
                     KFNF"
     gene            6476..8020
                     /locus_tag="K8M77_001578"
     CDS             6476..8020
                     /locus_tag="K8M77_001578"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBI7086073.1"
                     /translation="MSTSEIQKEQQSNLQSNLWNIANDLRGNMDASEFKNYILGLIFY
                     RYLSENVESRANKLLEEDGVSYEEAWEDEELREALKEELVNDIGYFIEPKFLFDKLLA
                     KIETGDFDIEILEEAINNITESTLGQESEEEFDHLFDDMDLKSTKLGKDVKSRSELIA
                     KVMGKIAQIDFRFDNSEIDILGDAYEYLIGQFAANAGKKAGEFYTPQQVSKILAKIVT
                     MGKTDLKNVYDPTCGSGSLLLRVSREAKVRMFYGQEKTSTTYNLARMNMLLHGVKYSD
                     FNIKNDDTLENPQHGDLKFEAIVANPPYSAKWSGDDKFLDDERFSAYGKLAPKSKADF
                     AFIQHMIHHLEDNGTMAVVLPHGVLFRGAAEGVIRKHLIEQRNVLDAVIGLPANIFFG
                     TSIPTVILVFKKNRKNADNIMFIDASNEFEKGKNQNVLRDRDVEKIVETYKKRENVDK
                     YAYVATMEEIKENDYNLNIPRYVDTFEEEEEIDIIEVQKNIKELDKEIEELKKSLEAD
                     LKELGL"
     gene            8032..9222
                     /locus_tag="K8M77_001579"
     CDS             8032..9222
                     /locus_tag="K8M77_001579"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBI7086074.1"
                     /translation="MSRNVPKLRFKGFEDEWENYKLGSFSEKITEKNKGFKVKNVISN
                     SAKNGLISQRDFFEKDIANKNNIDGYYIIKNGDFVYNPRKSNEAPYGPINKYKFKDDG
                     VVSPLYFCFRINNKVSNECLEEYFKSSKWYRYVHMNSDQGARHDRVSIKDSEVMNMKI
                     KLPSLQEQEKIANFLSKVDSIIEKQEKKVEYWNSYKKGMMQKIFSQKIRFKDDNGRDY
                     PEWKEKKLGEICIIVMGQSPSSASYNDDNIGVPLVQGNADIYNKITKPRLYTSEITKI
                     CNENDIIMSVRAPVGSIAIADRNCCIGRGVCAIKSKENQSFLYQFLLDKEDKWGKISQ
                     GSTFESINSNDIKRLKIMIPNIKEQEKIAEFLLNIDSLVQKETKELEELRILKKGLLQ
                     QIFI"
     gene            complement(9287..11806)
                     /locus_tag="K8M77_001580"
     CDS             complement(9287..11806)
                     /locus_tag="K8M77_001580"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012492.1,HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7086075.1"
                     /translation="MIEKLAEDALKTYYFWKLYKKVSLNFIHNTLYLQNIKLTNKELT
                     DILRFADIFSNSKNEKYRNISYKIISLLYDTHKNNLTYNTYSNAVLKKLNNFPALKNS
                     LNIELPISRELQYLVDKEILKSPLCDDRYFLPNQYIVYKHLQKGKSLTFSGPTSMGKS
                     FVIKQFILEMISSKKSIYNFCIIVPTRALIKQYVSEFNAEFQKLKINSHKIVTNANIL
                     EFLDITTNKFVFILTQERLNVLLYTPYNIKVQYLIVDEAYKIFDDDTRGLTFYNTIDT
                     CLSKYKDIKILFSSPLIDNPEIFKNTFKPSIECYKSNESPVSQNLFYVDLKEKTVENI
                     DELIEDDPIKITNFIGNRNEFFYKIGAGSNNIVYLSGQDRAITYSLDFVDYLQNHNIN
                     LLNEEETNILNNLCILIEKNIHKDFFLINALKKGVAYHYGKLPTIVRENIEILFKKGI
                     IKYLFCTSTLIEGVNLPAKNLFIMANYNGIRKFKPLEFWNLAGRAGRLGYEYYGNIFC
                     VNDHSHNRAWKTKDILYTKNSLKIEDVLENKIINDKDKIIETISNDEPVIDLGKNEKY
                     TNYLANIIQIDNISNNNSIILEKATKVEPKLLNINNNKSDILDYNLINSNKTIDYKIQ
                     SSMSSKDSLPIITKIDYSTCLKMLQFMYTNYKWNIKEDRLKNIKFLNYIALLMNSWIN
                     GRPLNEIIESSINYYANNNKNIDTGSRQFKRFMKNNPIHVNALINQIIDNIENILRFD
                     LEKYFNHYYNLLKLKYSDDSIGCNIALYLEFGTRNTHEIILQNSGFTRYTSHILYKYY
                     SHHLKFNENTFDGINKNILSDSNLKKDSILYNEITNSSYIL"
BASE COUNT         4588 a         1201 c         1963 g         4099 t
ORIGIN      
        1 taagttatat ctcctaccca tttctcattt atgcttgttg ttgtaaaatc acgtttcaaa
       61 aggttttcta aaccttcttt cggctttttc ttagatgttg gtctatattt ttttattatt
      121 atagatttta ttcctaattc tctcaataat ctttgagttc tttttataga tatattaaat
      181 ccatttttag aaagcattat ttttatattt ggagctccgt atctacactt actttcttta
      241 taatacttta atatagccgt ttttagcttt tcattctcaa tttctctttc tgatttctta
      301 ggatttttct tactataata tgtgctacgt ggaacgttta aaatagagca cattttttta
      361 actccataag tttctttatt gacttctata acttgtataa ttttatttag ttctctttcg
      421 caaatatggc catagctttt ttagctaaag cttgcttcgc tataatattt cattttcctc
      481 tttaattcta gccatttctt tctttaattt ggctacttct tttaatgtta taacctcgtc
      541 ttcagatatt tttatttctt tgttagcttt tacccagcca tttatagttg attttgctat
      601 gccatattca ctacttattt cagaaattgt cattccagat ttatataaat ctgatatcat
      661 ttctttataa tcttctgtat attttttacc tctacccata gtagaaacac ttcctttctc
      721 taactaaata ttattttatc tagttcgata aaatgtgtcc actattttat actaacacca
      781 atatagatga agtacctaat tatagaatag agaataatgc aagggttgaa gcttataggg
      841 tggaaccagg agacttaata atttcaagta gaggagcaac aaaggttgct attataccag
      901 aaaatagtga aaatttatta atttcacaaa atttcatagg tttaaggctt aataatgaaa
      961 atgatccaga gtatattaag gaacttctac taagtcctat aggaagatac tttatagata
     1021 gaattaaaac aggaactgtg gttcaggtta ttaatcaaaa agattttgat gaaatgcctt
     1081 tagtatgttt taatcatgag aaacaaaaaa agattatgaa agagtataaa gaagaagaaa
     1141 aggaacttaa taaaaaaata gaagaactta aaagaaaact tgagaatatg aaacttaatt
     1201 tatatgaaaa aatgaagatt aaagatggct ttgaaatact ataagctata tgagaaagga
     1261 gatttccttt ctcataatct aaaaaatatt gagattttac atttaagtta ggattttcgt
     1321 atagtaaata gaaataagaa ataagagatt ggttaaagaa gtgtatttat aatgagagtg
     1381 ttattttgta atgtagcgtg gatgaaatat tatgatggaa taagtgagga tgataaaccg
     1441 attaatggtg gtaagtttat atatgaaaat gaagatggag cagaaatata taattttacc
     1501 ttatatagtg atgataattt tcatggtttt gtatcaacta aatcaaatag aggaagacag
     1561 aatcaattgc atatagagag atttgaagga gttagtgaag atgatgagga agcagataat
     1621 gttttagtca tttgggtagc taaagatcct gcaagaggta agagctacat agttggttgg
     1681 tacaaaaatg ctactgtttt cagaaattat tattatgatg aagatttatg tcctaaaaac
     1741 atatatgcaa atgcaaaaga ttgtgtacta ttaccaatga ataaaagaca aaagatagta
     1801 ccaagagcag gaagagatgg gtatttatat ggttttggaa gggctaatgt atggtttggt
     1861 aaaaagggag atgaaaaagc taatgaatat attaaaaata tggtagaata tataaacact
     1921 tatgatggag aaagcttaat ttaaatgtaa tttatattga atatattata ttaaatattt
     1981 aaagaataat taattgtata aatttaagat agagaggtgt tgagttatgg ataaattata
     2041 tgttgatttt ataaatgaaa taactaaaga agaattatat gaaggattgt tggcttatgg
     2101 gttgttttca gataaacttc caccgttttt atcatctaaa ggattttatg actattgtaa
     2161 aaatttaaac catgaattta aagcagggga aaaacaatat atatattatg agagtataag
     2221 aaatattaat tttcctagac cactaggaat tccaaatcca atggcatatc aaaaactttg
     2281 taaatgttta tcagaaaaat gggataaaat acaagagcat tttaaaaaga tgactgagaa
     2341 tcaattatat aaagttagta ggatacatat tagaaaatta aataattcaa ggaaattatt
     2401 tgagatgagt tataataatt ggatgagtga tggaacacct gaattagatt tattaattgg
     2461 aaaacgttat atggtaaaag cagatatatc taattgtttt ccaagcatat atacacattc
     2521 attaccatgg gcattagtag gtaaagagac tgctaagaag aatagaaacg ggaaaaaatg
     2581 gtttaataaa atagaccgtt gtgctcaaaa tataaagaat ggtgaaacac atggtttgat
     2641 aataggtcca catacgtcta atttgctttc agaaataatt ctatgtttta tagataataa
     2701 attaaataag tgggattata ttagaaatat tgatgacttt acttgttatg ttgaaacata
     2761 tgataaagca caagaatttt tggttgaact aagcaaagag cttagagagt ttgatttagt
     2821 actcaatcat aaaaaaacag aaataataga attgccaata gaatcaaaag aactgtggat
     2881 aaataaactt aattcagcat cattgttact aaaagggcgc aaagtaaatt ataatgaagc
     2941 aagattatat ttagatttga ctattgattt atttaaatgt aataaaatga attctgctat
     3001 tttaaattat gctattaaag tcttaggaag ttttgagtta acatataatg ctaaacaata
     3061 ttgtttaaaa acaatttttc attggacatt aatttatcca tatttagtta ttattttaga
     3121 aaaaaatgta tttaatgtat tttcagcaga tatttctcaa attaatgaaa tttcacagag
     3181 aatttttaaa aatggattag aaaatagaaa ttatgaagca gttagctatg ctatatattt
     3241 tgctataaaa tataaatttg aactcagtga tatatgtgct tatgatgcaa taaaaagtga
     3301 tcattgtatt tataaacttc ttacttatat atactttgcg aaaaataatg ataataaatc
     3361 aaaagaatta ttagaagatc atgctaaaaa cttaaagagt aatgatgagg actttaatag
     3421 aaattggtta tttgtgtatg aggttttgcc agttgattgt tttgaagatg aatggaaagc
     3481 aataaagaga aataatgttt catttattaa catataaata cattgaaaat aatctaattt
     3541 aggatatgac taaattattt ttaatattaa tctattcttg atataatatg tatataaata
     3601 gtagattaac aaaattttgt tttaaataga ttagaaggaa gagagttttt ttataatgac
     3661 ttatcaaagt gaacatgaat tagaagaatt attaatcaat caattagttg agcaaggcta
     3721 tgcaatagtt aatatacctg atgaagctgc tttagagaag aattttaggg agactttatt
     3781 tgagtttaac aggaaaaaac taggtggtgc ggcttttaca gacaaagagt ttgaaagaat
     3841 acttattcat ttaaagaata agagtatttt taatagtgct aagattttta gggacaagtt
     3901 catccttgag agagagaatg gaactaaggt ttacatagag ttttttgatg gaaaggatta
     3961 tcacaacaac atattccaag tttctaatca gataactatg gaaggtaaat atgttaatag
     4021 gtatgatgtt actatactta ttaatggctt acctatggtt cagattgagt taaagagaag
     4081 gggcaaggac ttttatgaag cctttgatca aatagagaga tatagaagac attctttgaa
     4141 gggtttattt agatatatcc aatgttttgt tattagtaat ggtgttgaca ctaaatatta
     4201 ttctaatagc gataaggaaa ttaagcgtga gtttactttt ttctggagtg atgaaaagaa
     4261 taagagagta actaacctta gagaatttgc tgaatctttt ttagatagaa atatgttaaa
     4321 taagatcata gctaggtata tgattattaa tgagagtgaa aaaaatctta tggttatgag
     4381 accttatcag atatatgcag ttgaagcttt aatgaagagg gctaatgaaa ctaacaacaa
     4441 tgggtttatt tggcatacta caggttctgg aaagacctta acttcattta aggcaagtca
     4501 gcttttagca gatgaggaca aggttaagaa ggtatttttc ttaatagata gaaaagattt
     4561 agacagccaa actgttgagg agtttaataa gtttgagaag gattgtgttg atacaactga
     4621 taatgttaga actttaataa aacaaataga ggatgtaaac acaaggctta ttgtaactac
     4681 catacaaaag ttagctaggc ttttaaaatc agagaaatat gcacatagaa tggacaagta
     4741 tagagatgag aaagttatct ttataataga tgagtgtcat agatctcaat ttggagagat
     4801 gcacacagct ataaataagc attttacaaa ggctcaatac tttggattta caggaacacc
     4861 aagacttcca gagaatagaa gtcaggatgg aagaactaca gcggatattt ttgataaatg
     4921 cttacatcat tatttaataa aagatgctat taatgatggc aatgtacttg gattttctgt
     4981 ggagtatatt aaaacctttg atggtcaggt taatgagtat gatgatgaat atataaaggc
     5041 tatagacaaa gaagaagtct ttatgaatga tgaacgtata gagaatatag ctaaggatat
     5101 aatagctaat catgataaaa agacagcagg aagaaagttc tgttcacttt ttacagttca
     5161 aaagattcca atgcttgtta agtattatga tgcttttaaa gctttagaac atgatttaaa
     5221 aatagcagca atttttacct atgatgataa tgaaaattta gatggtaagt gtgaacattc
     5281 aagggagagc cttgatggaa ttataagtga ttataacaag atgtttaagg aggaggatat
     5341 agatttttca acagatactt ttaaggagta ttcaaagcat ctatcaaaaa agattaaaaa
     5401 tgcagaggtt gatatagtta tagttgtaaa tatgttttta acaggatttg atagtaaacc
     5461 acttaatact ctttatgtgg ataagaattt aaaatatcat ggacttatac aagcatattc
     5521 aagaactaac agaatattag atggcacaaa acagcatggt aatatagttt gctacagaaa
     5581 tttaaaaaag gaaactgatg aagctatatg tttattttca caaactgata gtacagatgt
     5641 ggttttaatg gaaagctttg atcattattt aaagcttttt caggaaagac tagatagatt
     5701 atatgagact gcaccaacaa tagaatctat tgactctatg caggatgaaa atgaaaagaa
     5761 agattttgta gaggctttta gggaattaac aaagcaatta actaagctta aaacctttgt
     5821 tgagtttgag tttaatgaaa aaaagctagg aatatcagct cagaaatatg aagattataa
     5881 gagtaaatat tttgctatat atgaaagtgt taagaaagat gggaaaacct ctgtattagt
     5941 agatatagac ttttgcatag agcttatgca aacagataag ataaatgtta actatatact
     6001 aaacctaata agaactatag attttgagac agaagagaaa accaagaagg atatagagaa
     6061 aattaagagg ttattagata atgctgataa tgaaaattta agacttaagt cagaattact
     6121 tagagagttt ttagatcagg ttgtacctaa tattacaaaa aatgactcag tggatgaagc
     6181 ctttaacgaa tttttagaag acaagagagt agatgaaata gaatactttt caaatgaagt
     6241 tgagatagat aagtttaagg ttaaggaata tgtttcagaa tatgagtatt caggaagaat
     6301 tgatggttct gaaataaaag ataatttaag tggtggtctt ttaaagaaga agagacttgc
     6361 agaaaagata aaaactttca taataaatca tgttaccaag tttaattttt aagtgttata
     6421 ataatgtgaa agtaaaaaga tttagaaatg ttatatggaa agtagggaag acataatgag
     6481 tacatctgaa atacaaaagg aacagcaaag taatttacaa agtaatttat ggaatatagc
     6541 aaatgattta agagggaata tggatgctag tgagtttaaa aactacatac taggtcttat
     6601 attctataga tatctttcag aaaatgtgga aagcagagct aacaagcttt tagaagagga
     6661 tggcgttagc tatgaagaag cttgggaaga tgaggagctt agagaagctc ttaaggaaga
     6721 gttagtaaat gatataggat attttattga gcctaagttc ttatttgata agcttttagc
     6781 taagatagaa actggtgatt ttgatataga aatcttagag gaagctataa ataatataac
     6841 tgaatctact ttaggacaag agagtgaaga ggagtttgac catctttttg atgatatgga
     6901 cttaaaatca actaagcttg gtaaggatgt taagagtaga agtgagctta tagctaaagt
     6961 tatgggaaaa atagctcaaa tagattttag atttgataac agtgaaatag atatacttgg
     7021 agatgcttat gagtatctta taggacagtt tgcagctaat gcaggtaaga aggcaggaga
     7081 gttctatact cctcagcagg tttcaaaaat attagctaaa atagttacaa tgggaaaaac
     7141 tgaccttaaa aatgtatatg atccaacctg tggatcaggt tcattattac ttcgtgtttc
     7201 tagagaagca aaggttagaa tgttctatgg acaagagaag acttcaacaa catataactt
     7261 agcaagaatg aatatgcttc ttcatggagt taaatatagt gatttcaata ttaaaaatga
     7321 tgatacttta gagaacccac aacatggaga tttaaaattt gaagcaatag ttgcaaaccc
     7381 tccatattca gcaaaatgga gtggggatga taagttctta gatgatgaaa gattttcagc
     7441 ttacgggaaa ttagctccaa aatctaaggc agactttgca tttattcaac atatgataca
     7501 tcacttagag gataatggaa caatggcagt tgtgcttcct catggagtat tatttagagg
     7561 ggcagcagag ggagttataa gaaagcatct tatagaacaa agaaatgttt tagatgcagt
     7621 aattggactt ccggcaaaca tattctttgg aacatcaatt ccaacagtaa tacttgtatt
     7681 taaaaagaat agaaaaaatg cagataatat aatgtttata gatgcatcaa atgaatttga
     7741 aaaaggtaaa aaccaaaatg tcttaagaga tagggatgta gaaaagatag tagaaacata
     7801 caaaaagaga gaaaatgtag ataaatatgc atatgtagct actatggaag aaataaaaga
     7861 aaatgactac aacttaaata taccaagata tgtagatacc tttgaggaag aagaagagat
     7921 agatataata gaagttcaaa aaaacataaa agaactagat aaagaaatag aagaactaaa
     7981 gaaaagtcta gaagcagact taaaagaatt aggtctttag gaggtacaag gatgagtaga
     8041 aatgtaccaa agttaagatt taaaggcttt gaagatgagt gggaaaatta taaattagga
     8101 agtttttcag aaaaaataac tgaaaaaaat aaaggattta aagttaaaaa tgtaatatct
     8161 aattcagcta aaaatggttt aataagtcaa cgggattttt ttgaaaaaga tatagcaaac
     8221 aagaataata tagatggata ttacataatc aaaaatggtg attttgttta taatcctaga
     8281 aaatctaatg aggctccata tggaccaatt aataaatata agtttaaaga tgatggagta
     8341 gtttctccac tatatttttg ttttaggata aataacaaag ttagtaatga atgtttagaa
     8401 gaatacttta aatcgtctaa atggtataga tatgtgcaca tgaatagtga tcaaggggct
     8461 agacatgaca gagttagtat aaaagattca gaagtaatga atatgaagat taaattgcca
     8521 tcattacaag aacaagaaaa aatagcaaat ttcttaagta aagtagatag tataatagaa
     8581 aaacaagaaa agaaagtaga atattggaac tcttataaaa aaggaatgat gcagaaaata
     8641 ttctcacaaa agataagatt taaagatgat aatgggagag attatccgga gtggaaagaa
     8701 aagaaattag gagaaatatg tatcatagta atggggcaat ctccatcttc agcatcatat
     8761 aatgatgata atattggggt acctttagtg caaggaaatg ctgatattta taacaagata
     8821 acaaaaccta gattatatac aagtgagatt actaaaattt gtaatgaaaa tgatataata
     8881 atgtcggtta gggcgcctgt tggaagtatt gcaatagcag atagaaattg ttgcatagga
     8941 agaggcgttt gtgcaataaa atctaaagaa aatcaaagtt tcttatatca atttttatta
     9001 gataaagaag ataaatgggg aaaaatatca caaggatcta catttgagtc tattaatagt
     9061 aatgatataa aaagattgaa aattatgatt ccaaatataa aagaacaaga aaaaatagct
     9121 gaatttttat taaatataga tagtttagtt caaaaagaaa ctaaagaact agaagaatta
     9181 agaattttaa aaaaaggatt attacaacag atatttatat aaatataagc aaggatatag
     9241 gaatatatcc ttgcttttta aaattaacac ttataattct taagcattat aatatatatg
     9301 aggagttagt aatttcatta tataaaatgc tatctttttt tagattagaa tcagataata
     9361 tatttttatt aataccatca aatgtatttt cattaaattt caagtggtgt gaataatatt
     9421 tatataagat atgactagtg tatctagtga aaccagaatt ctgtaatatt atctcatgag
     9481 tatttcttgt accaaactca agatataatg ctatattgca gccgattgag tcatcggaat
     9541 attttaattt taatagatta taataatgat taaaatattt ttctaaatca aatcttaaga
     9601 tattttcaat attatcaatt atttggttta taagtgcatt aacatgtata ggattattct
     9661 tcataaatct tttaaattgt ctagagcctg tatctatatt tttattattg tttgcataat
     9721 aattaataga agattctatt atttcattta aaggacgacc atttatccat gagttcataa
     9781 gtaatgcaat ataatttaaa aattttatgt tttttaatct atcttccttg atattccatt
     9841 tataattagt atacataaat tgtaacattt ttaaacaggt tgaataatct atttttgtaa
     9901 ttattggtaa agaatcttta gaagacatac ttgattgtat tttataatct atagttttat
     9961 tactatttat taagttataa tctaatatat ctgatttatt attgtttata tttagtaatt
    10021 taggttcaac tttagttgct ttttcaagta ttatactatt attattggat atattatcaa
    10081 tttgaataat gttagctaaa taatttgtat atttttcatt tttgcctaag tctataactg
    10141 gctcatcatt agatatagtt tctattatct tatctttatc attaataatt ttattttcta
    10201 aaacatcttc tatttttaga ctgtttttag tatagagtat atcttttgtt ttccaagctc
    10261 tattatggct atgatcatta acacaaaaaa tattaccata atattcataa cctaaacgtc
    10321 cagcacgtcc ggctagattc caaaattcta aaggtttaaa ttttctaata ccgttgtagt
    10381 tagccattat aaataaattt ttagcaggta ggttaacacc ttcaataagt gtacttgtac
    10441 aaaataaata tttaattata ccttttttaa ataatatttc gatattttca cgtacaatag
    10501 tagggagttt accataatga taagctacac ctttctttaa tgcatttatt aagaaaaaat
    10561 ctttatgtat attcttttca ataagaatac atagattatt aagtatattt gtttcttctt
    10621 cattcaataa attaatatta tgattttgta gataatctac aaaatcaaga gagtaagtta
    10681 tagcacgatc ttgaccagat aaatatacaa tattatttga accagcgcct attttataaa
    10741 aaaattcatt tctatttcca ataaaatttg tgattttaat aggatcatct tcaataagtt
    10801 catcaatatt ttcgactgtt ttttctttta aatcaacata aaataaattt tgtgaaacag
    10861 gtgattcatt tgatttataa cattcaatag aaggtttaaa agtattttta aatatttcag
    10921 gattatctat taaaggtgaa gaaaataaaa tttttatatc tttatacttt gaaagacaag
    10981 tatcaatagt attataaaat gttaatccac gtgtatcatc atcaaagatt ttataagctt
    11041 catcaactat taaatattgt acttttatat tatatggtgt atataatagg acattaagtc
    11101 tctcttgggt caaaataaat acaaatttat tagtggttat atctaaaaat tctaagatat
    11161 ttgcattagt tactatttta tgactattta tttttaattt ttggaattca gcattaaact
    11221 cagatacata ttgctttatt agtgccctag ttgggactat tatacaaaaa ttatatatgc
    11281 tttttttaga ggaaatcatt tctaaaataa attgttttat tacaaaggac ttacccatag
    11341 atgttgggcc tgaaaaggtt aatgatttcc ctttttgcaa gtgtttgtaa acaatatact
    11401 ggtttggaag aaaatatcta tcatcacata atggtgattt tagtatttct ttatctacca
    11461 agtattgtaa ttctctagaa ataggaagtt caatatttaa ggaatttttc aatgcaggaa
    11521 aattgttcaa tttttttaat actgcatttg aatatgtatt atatgttaaa ttatttttat
    11581 gtgtatcata taataggcta attattttat atgagatatt acgatatttt tcatttttag
    11641 agttagaaaa aatatctgca aatcttaata tatcagttaa ttccttattt gtgagtttaa
    11701 tattttgtaa atatagggta ttatgtataa agtttaaact tactttttta tataatttcc
    11761 aaaaataata tgttttaagg gcatcttctg ctagtttttc aatcatattt tcctcctatg
    11821 ataaaatttc ttctaatatt ttaatttttg t
//
LOCUS       DAEVGI010000072        11406 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEVGI010000072 DAEVGI010000000
VERSION     DAEVGI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.072"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(77..1183)
                     /locus_tag="K8M77_001581"
     CDS             complement(77..1183)
                     /locus_tag="K8M77_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI7086076.1"
                     /translation="MFKKHNFHFNWLPLILISTVSIMLIFNIRSILSEGIRPFLSVLT
                     PFFWAFGVAYLINPLMMLIEKRFKLKRAFSLMISYILVIICVYILIAIIVPTIFDSLS
                     ELFSNLSEYASSAESWINSTLASMNVPLDLNHLNTSATEFISKTSTLLSNIVGSLLTQ
                     TIKITSSVVKFVFGFIISIYMLMDKETLIGGCKKIIIAVMPSEKSSELFISFMAEAHN
                     IFSRFIIGKIIDSLIIGVLCYIGLLLMGTQYAVLISFIVGLTNMIPYFGPFIGMIPAF
                     LLTLIVQPSMAIWVLLFIFILQQFDGWYLGPKILGDQVGVSPLFIIFAVTVGGGIFGV
                     LGMFLSVPIIALIKIYTEKLIEYRLENKKQNFSE"
     gene            complement(1248..1778)
                     /locus_tag="K8M77_001582"
     CDS             complement(1248..1778)
                     /locus_tag="K8M77_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBI7086077.1"
                     /translation="MYDSTLELAENKLLLLYIIKSIKYPISNTQLTDIVLENSFINYF
                     MLQQYVSELISSEFLEYKTQDDDKEIIVLTEKGDKVLFFFKDRISPNKLNLIDEYVRK
                     HVEKIKKELSISADYTVGEDSSFIVNLKAIENNTILIDLNVSVPSKTEAKAICNKWEN
                     NSSDIYNKIIHALIED"
     gene            1886..2662
                     /gene="pdaB"
                     /locus_tag="K8M77_001583"
     CDS             1886..2662
                     /gene="pdaB"
                     /locus_tag="K8M77_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family sporulation
                     protein PdaB"
                     /protein_id="HBI7086078.1"
                     /translation="MKKNNNFFIFKNFFNTIILVVIIAIFTLPINVFAKDNKPIHCVD
                     RDDKKISLTFDVNWAENDYLYEILDLLDKNNVKATFFIMGKWVIYPEGNREKLIEIHK
                     RGHEIGNHSYVHPDFKNIGKERIIEEVKKTEEIIEKEVGVKTNLFRFPSGSYSQESLS
                     VIHGLQYKSIQWDIDSVDWKGESKEREYKKVIDNIKGGSILLYHNNGKHTVENLNEII
                     PKLKSEGYEFVKISDIIYENSYEIDDNGKQLLIKTKQNNS"
     gene            2778..3434
                     /locus_tag="K8M77_001584"
     CDS             2778..3434
                     /locus_tag="K8M77_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI7086079.1"
                     /translation="MDNLMLNNKIYLEGTVVSELEFSHEMYGEGFYTFNLEVMRLSDS
                     KDLLNITVSERLITTMDLKIGTEILVEGQLRSYNKFIDGNNKLILTVFARNIEYCEER
                     SKNPNEIFLDGFICKEPVYRTTPFGREIADLLLAVNRSYNKSDYIPTIAWGRNSRFCK
                     TLEVGDNIRIWGRLQSREYQKKISETEVVKKIAYEVSISKMEKVSVESEQNESSEEIS
                     "
     gene            complement(3483..4193)
                     /gene="dapD"
                     /locus_tag="K8M77_001585"
     CDS             complement(3483..4193)
                     /gene="dapD"
                     /locus_tag="K8M77_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBI7086080.1"
                     /translation="MSYNFTDPYEIARFIKEVKKSTPVKVYLKGNLEGVELGSIECYG
                     NNDFYVLFGESDEVATFLTENKDKIVSFRLENDRRNSAIPMLELLNINARIEPGAIIR
                     DRVSIGDNAVIMMGAVINIGAEIGESTMVDMNAVIGARGKLGKRVHLGAGAVVAGVLE
                     PPSKTPCIIEDDVLIGANAVILEGVKIGKGSVVAAGSVVVEDVPAGVVVAGTPAKIIK
                     SVDEKTKDKTEILDDLRK"
     gene            4386..4922
                     /locus_tag="K8M77_001586"
     CDS             4386..4922
                     /locus_tag="K8M77_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI7086081.1"
                     /translation="MARLKEGYVQVYTGNGKGKTTAAMGLAFRAAGDGMEVKVVQFLK
                     SWKTGELESAKRFDNLEILRFEKVKGFTWELNEEELAQLKSEVRVGFDFVKGLIETRG
                     CDILILDEVMASISGGFIGEDEIVELIEKKPKDMELILTGRNVPEKIMEKADLITEMR
                     EIKHYYKKGVPAREGIEF"
     gene            4922..6064
                     /locus_tag="K8M77_001587"
     CDS             4922..6064
                     /locus_tag="K8M77_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI7086082.1"
                     /translation="MNKNVIGVEISGIRKFYNEVVKFPEAISLTLGQPDFPVPEKVKE
                     AMIRAIEEGKTTYTANAGIVELREEISGLLKNTFDIDFSKDEIIITVGGSEGLYAAMT
                     AILNPGEKVLVPSIAYPAYESISKIIGCEVINYDLNEDFSVNIESLKEGIKQGGKLLV
                     LSYPCNPTGALLSKKSRDELVEIIKENDILVLTDEIYSSLCFEEEYYSVAQCKDIKEK
                     IIYVSGFSKMFSMTGLRIGYVACSKEIYDQIIKVHQYNSSCATSISQWGALEGLKSCM
                     NDVENMKESFKERMNFTYKRLKSMGLEVEKPKGAFYIYPNISKFGLTSEEFCHRLLKE
                     GKVACVPGDAFGKGGEGYIRISYCYSKDELERALDKLEAFVKILKK"
     gene            complement(6372..7136)
                     /locus_tag="K8M77_001588"
     CDS             complement(6372..7136)
                     /locus_tag="K8M77_001588"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBI7086083.1"
                     /translation="MVKVILNGCSGKMGSVVSNLAETKFPNVEIVAGIDNNTMAQRSY
                     PIFAKPEDCNVSYDVLLDFSRADALKSLVEFSKKTKKPLILCSTGYTAEDLKFIEESS
                     KEIPLFRSANMSIGINLVNNLLKKVAPVLYENFDIELVERHHNQKVDAPSGTALLLAH
                     TIQDSLNEETKLLYGREGIAKREKNEICVNTVRGGGIIGDHEVIFAGDGEVIEINHKA
                     ISRDVFAIGALKACEYMADKTKAGKYSMDDVLQLNF"
     gene            complement(7209..8084)
                     /locus_tag="K8M77_001589"
     CDS             complement(7209..8084)
                     /locus_tag="K8M77_001589"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBI7086084.1"
                     /translation="MFKGSCVALITPFIEDGVNYEELRKLLEWHIKNHTDAILVCGTT
                     GEGSTMTLEEKKEVIKFSVEVVNKRVPVIAGTGTNNTKASIELSKYAEEVGADMVLII
                     TPYYNKTSQKGLYAHFSAINDAINIPIMLYNVPSRTGMNITPLMLDKLANLNNVVAIK
                     EASGDLSQVAKMAELCGDRIAIYSGNDDQIVPILSLGGAGVVSVLANILPEETHNICE
                     KYFLGEVIESRNLQLKYLSLANSLFIETNPIPVKTAMNLMNFNCGQLRLPLCEMEDSN
                     LVILEENLKANGLIK"
     gene            complement(8109..9086)
                     /locus_tag="K8M77_001590"
     CDS             complement(8109..9086)
                     /locus_tag="K8M77_001590"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI7086085.1"
                     /translation="MYNVAIVGATGNVGRKFLEILEERNFPVKELYLFASKRSAGKTL
                     KFKGEDVLVEELCEGNIENKKIDFALFSAGGSVSLEFAPIFAKHGAVVIDNSSAWRMD
                     KEVPLVVPEVNPEDVKWHKGIIANPNCSTIQAMVALKPLYDKYGIKRIVYSTYQAVSG
                     AGIQGILDLQEGTTKKFPYPILGNVIPHIDVFLDNGYTKEEIKMIEETKKILHDDNLR
                     ITATTVRVPVLNAHSESINVELNSEFELENVVDLFNNSKGIIVHDDVENLKYPTPLEL
                     SGKDEVFVGRIRRDFSLDNGLNLWVVADNIRKGAALNAIQIAEILINEK"
     gene            9288..9560
                     /locus_tag="K8M77_001591"
     CDS             9288..9560
                     /locus_tag="K8M77_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBI7086086.1"
                     /translation="MSKTPLKKIIKGKIKSNKELTPAEKLREKMKYEIADELGLSDKV
                     DKFGWGGLTAEETGRIGGLMTKRKKELNLPSNDEILGRKKPHVDEK"
     gene            9612..10349
                     /locus_tag="K8M77_001592"
     CDS             9612..10349
                     /locus_tag="K8M77_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI7086087.1"
                     /translation="MKSYKMMAKVYDELIYEDVNYENIAEYALSKCDKYNINKEMYLD
                     LACGTGNVGVHVGKSFKENYFVDLSQDMLIEADKKLREHRVRGKIVCQDMCDLGLNRK
                     FDLITCVLDSTNYILDDESVENYLRGVYNHLKEDGLFVFDINSYYKISEVLGNNVYTY
                     DSEELFYAWENIFEDEIVEMNLNFFVKDGEKYERFTEIHEERAYKESEIEHILDVIGF
                     KILEKHNGYTEDEVTPDTERIVYIVKK"
     gene            10365..11324
                     /gene="hslO"
                     /locus_tag="K8M77_001593"
     CDS             10365..11324
                     /gene="hslO"
                     /locus_tag="K8M77_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBI7086088.1"
                     /translation="MSDKLIRAIAKDGMIRIFATETTELVNEASKIHDCTPTAAAALG
                     RMLTAGTMMGAMLKSDKEVVTLQINGGGMAKGVTVTAYSDCSVKGYIGNPHVDLPLNT
                     ENGKLNVGEAIGKNGGLTVIKDLGLKDPYVGQVPIYSGEIAEDLAYYFTASEQIPSAV
                     ALGVLVDRDHSIKKAGGFIIQLLPGADELLGDLLTYRLDEIPSLTTMLSEGKTIEEVI
                     EFIFDGMDLKILEEETPKYKCDCSREKVERALLSIGYKDLKELYDEGKEEELKCHFCN
                     KAYKFTKEDIGKLLEEKEKSIADEVSEEMKKAEEKEKKEKNKK"
BASE COUNT         4333 a         1414 c         1787 g         3872 t
ORIGIN      
        1 ttaaataatt attaacaatt agttaaataa taaatataaa aaaataaaga ggatattata
       61 aaatatcccc tttatattat tcactaaagt tttgtttttt attttctaat ctgtattcaa
      121 ttaatttttc cgtatatatt tttattaagg ctattatagg aacacttaag aacattccta
      181 aaaccccaaa tattccgcca ccaactgtaa cagcaaatat tataaatagt ggagaaacgc
      241 ctacttgatc tcctaatatt tttggaccta agtaccaacc atcaaattgt tgaagtatga
      301 atatgaataa aagaacccat attgccatag agggttgaac aataagtgtt aataaaaatg
      361 caggaatcat tcctataaat ggaccgaaat atggaatcat atttgttaat ccaactatga
      421 aagaaataag aaccgcatat tgagtaccca ttaataataa acctatgtaa caaagaactc
      481 ctattataag tgaatctata attttaccta ttataaatct actaaagata ttatgagctt
      541 ctgccatgaa actaataaaa agttctgaag atttttcaga aggcataact gcaattataa
      601 tttttttaca tcctccaatt aaagtttcct tatccattaa catgtatatt gaaataataa
      661 atccaaatac aaatttaaca acagatgaag ttatttttat agtttgagtt aacaagctac
      721 ctactatatt agatagtaag gttgatgtct tagaaataaa ttccgtagca gaagtattta
      781 agtgatttaa gtctaaagga acattcatac ttgctaaggt gctattaatc caactttcag
      841 cagaagatgc atattcacta agattactaa ataattctga caaactatca aatattgtag
      901 gaacaataat tgcaattaat atgtaaacac atattataac taaaatatag cttatcatta
      961 aggaaaaagc tctcttaagt ttaaacctct tttctataag catcatgaga ggatttatta
     1021 aataagctac accaaaagcc caaaaaaatg gtgttaaaac tgataaaaat ggtctaatgc
     1081 cttcacttaa aatgctccta atattaaaaa ttaacattat tgaaactgtt gatatcaata
     1141 ttagtgggag ccaattaaag tgaaaattat gttttttaaa caattttgtt tccccctaaa
     1201 ttatataaat tttataacga tttttgctaa taataatagc aggattatta atcttctata
     1261 agagcatgaa ttattttgtt atatatatcg gaagaattgt tttcccattt gttgcaaata
     1321 gcttttgctt cagttttgga aggaacgctt acatttaaat caattaaaat agtattattt
     1381 tctattgcct taaggtttac tataaaagaa ctgtcttctc caacagtata atcagcagat
     1441 atacttaatt ctttttttat tttttcaaca tgcttgcgta catattcatc tataagattt
     1501 aatttgtttg gagatattct atctttaaag aagaatagta ccttatctcc tttttccgta
     1561 agtactatga tttctttatc atcatcttga gttttatatt ctaagaactc agaagaaatt
     1621 aattcagata catattgttg taacataaaa taatttataa aactattttc taaaactata
     1681 tctgttaatt gtgtgttaga tataggatat tttattgact ttattatata taaaagaagt
     1741 aatttattct cagctaattc taatgtgcta tcatacattt atgttttcct ccataactgt
     1801 tttgaaataa taatcctatt ataacatata tgttttaaga taataaagtt atttgattaa
     1861 tattaaatac taaggagtga atactttgaa aaaaaataat aattttttta tttttaaaaa
     1921 cttttttaat accattattt tagttgttat tattgccata tttacattgc ctattaatgt
     1981 ttttgcaaaa gataataagc caattcattg tgtagataga gatgataaaa aaatttctct
     2041 gacctttgat gtgaattggg cagaaaatga ttatttatat gaaattttag atttactaga
     2101 taaaaataat gtaaaagcta cattttttat aatgggtaag tgggtaatat atcctgaagg
     2161 caatagagaa aagttaatag agattcataa gagaggacat gaaataggaa atcatagtta
     2221 tgtacatcct gattttaaga atataggaaa agagagaata attgaagagg ttaaaaaaac
     2281 agaagaaata atagaaaagg aagttggagt aaagactaat ttatttcgat ttcctagtgg
     2341 ttcatatagc caagaaagtt taagtgttat tcatggatta caatataaaa gtatacaatg
     2401 ggatattgat agtgtggatt ggaaaggaga gagtaaagag agggaatata aaaaggtaat
     2461 tgataatata aaaggtggat cgatactttt atatcataat aatggtaaac atactgtaga
     2521 aaatcttaat gagataattc caaaattaaa atcagaagga tatgaatttg ttaaaataag
     2581 tgacataata tatgaaaata gttacgaaat agatgacaat ggaaaacaat tgttaatcaa
     2641 aacgaaacaa aataatagtt gaaatcgttg aacaaaatgt attataatgg aaatagttat
     2701 tagatataca gataaatata aaaaacatat taaacttata tgattaaata ctaatgggga
     2761 gaggggttta ggttaaaatg gataatttaa tgttaaacaa caaaatttat ttagaaggaa
     2821 ctgtagtttc agaattagag ttcagtcatg aaatgtatgg agagggtttc tatacattta
     2881 acttagaggt tatgagatta agtgattcaa aggatttatt aaatattact gtatcagaaa
     2941 gattaataac cactatggat ttaaagattg gaactgaaat attagtcgaa ggacaattaa
     3001 gaagttacaa caaatttata gatggaaata acaaacttat tttaacagtt tttgcaagga
     3061 atatcgaata ctgtgaagaa agaagtaaaa atcctaatga gattttctta gatggattta
     3121 tttgtaagga gccagtttat agaacaactc catttggaag agaaattgcg gacttattat
     3181 tagctgtaaa tagatcttat aataaatcag attatatacc aactatagca tggggaagaa
     3241 actcaagatt ctgtaaaaca ttagaagtag gggataatat aagaatttgg ggaagacttc
     3301 aaagtagaga atatcaaaag aaaatatctg agactgaagt tgttaagaaa atagcatatg
     3361 aggtttcaat ttctaaaatg gaaaaagtat ctgttgaatc agaacaaaat gaatcaagtg
     3421 aagaaatttc ttaaaaattt taataagaat gccatgggaa taaattctca tggcattttt
     3481 tattattttc ttaaatcatc taatatttct gttttatctt tagttttttc atcaacagat
     3541 ttaataattt tagcaggagt tccagctaca actacgccag ctggtacatc ttcaactacc
     3601 actgatccag cagctacaac tgagcctttt ccaattttaa caccttcaag gataactgca
     3661 ttagctccta ttaaaacatc gtcttctatt atacatggag ttttgctagg tggttctaaa
     3721 actccagcaa caacagcgcc agcacctaag tgaactcttt ttccaagttt tcctctagca
     3781 cctattacag cgttcatatc taccattgta ctttcaccta tttcagcccc tatgtttatg
     3841 acagcaccca tcattataac agcgttgtct ccgattgata ctctgtctct tattattgcg
     3901 ccaggttcga ttctagcgtt tatgtttaaa agttctaaca ttggaatagc tgagtttctt
     3961 ctatcatttt caagtctaaa gcttactatt ttatctttat tttctgttaa gaaagtagca
     4021 acttcatcac tttctccaaa taatacatag aaatcattat ttccataaca ctctattgaa
     4081 cctaattcta caccttctaa gttaccttta agataaactt taacaggagt tgattttttt
     4141 acttccttta tgaatctagc aatttcatat ggatctgtaa agttataact catattaaat
     4201 tacatccttt ctatttataa tgcattttgt ctatatattt attaaaatat aacgtacaat
     4261 ataaattatt ataaacaaaa atgattatca ataaaagagg aaacttaaaa tttatattat
     4321 ttaataaata gattgtttaa aaaatcaaat ataatataaa atataattta aaggagtgat
     4381 aaaatatggc aagacttaaa gaaggatatg ttcaagtata tactggaaat ggaaaaggaa
     4441 aaactacagc agcaatggga ttagctttta gagctgcagg tgatggcatg gaagtaaagg
     4501 tggttcaatt ccttaaatca tggaaaacag gtgaattaga atctgctaag agatttgata
     4561 acttagagat attaagattt gaaaaggtta aaggatttac ttgggaatta aatgaggaag
     4621 agttagctca gttaaaatcg gaggttagag taggttttga ttttgtaaaa ggactaattg
     4681 aaactagagg atgtgatata ttaattttag atgaggtaat ggcttcaatt tctggtggat
     4741 tcatagggga agatgaaatt gtagaattaa ttgaaaagaa acctaaagat atggaattaa
     4801 ttcttacagg aagaaatgta ccagaaaaaa taatggaaaa agctgattta ataacagaga
     4861 tgagagaaat taaacattat tataaaaaag gagttccagc tagagagggg atagaatttt
     4921 aatgaataaa aatgttattg gggtagaaat atcaggaata agaaaattct ataatgaagt
     4981 agtaaaattc ccagaggcta tatcattaac tttgggacag ccagattttc ctgttccaga
     5041 aaaggttaaa gaagctatga taagggctat tgaagaagga aagacaacat atacagctaa
     5101 tgctggtata gttgaattga gggaagaaat atcaggatta ctaaagaaca cttttgatat
     5161 tgattttagt aaggatgaaa taattataac tgtaggtgga agtgaagggt tatatgcagc
     5221 tatgacagcc attttaaatc caggagagaa ggttttagtt ccatcaattg catatccagc
     5281 ttatgaaagc ataagcaaaa taataggatg tgaagttata aattacgatt taaatgaaga
     5341 tttttcagtt aacatagaaa gtttaaaaga aggaataaaa caagggggaa aacttttagt
     5401 actttcctat ccatgtaatc ctacaggggc attacttagt aaaaaaagta gagatgagtt
     5461 agtagagatt ataaaagaaa atgatatttt agtattaact gatgaaattt attcatccct
     5521 ttgttttgag gaggagtatt attctgtagc tcaatgtaaa gatataaaag aaaaaataat
     5581 atatgtaagt ggattctcta agatgttttc aatgacaggg cttagaattg gatatgtagc
     5641 atgttctaag gaaatatatg atcaaattat aaaggttcat caatataatt cttcatgtgc
     5701 aacatctatt tctcaatggg gagccttaga gggattaaaa tcttgtatga atgatgttga
     5761 aaacatgaag gaatctttta aagaaagaat gaattttact tataaaagat taaaaagtat
     5821 gggattagaa gtggaaaaac ctaaaggagc tttttatatt tatcctaata taagtaagtt
     5881 tggattaaca tcagaagaat tttgtcatag attacttaaa gaaggcaaag ttgcctgtgt
     5941 accaggagat gcctttggaa aaggtggaga agggtatatt agaatatctt actgttatag
     6001 caaagatgaa cttgaaagag ctttagataa attagaggct tttgtaaaaa ttttaaaaaa
     6061 gtaaactata tcttaaatat atgtcttaag taaaaaataa tttaaaaagg caaaaataaa
     6121 ataagttata taaaattaaa aataaaactt ataaatagta ttaagagaag aaaataattc
     6181 tcaaaatata tttataagtt ttattgtttt actttgtttt aaatattttt atttcttatg
     6241 aataatttaa attgaaaggt tctaaaagag tatataatct tttaaaatct tatttcagat
     6301 ctttaaattc gtacatagaa attaattata ttttaaaaca aaaagtgttt cagtatttta
     6361 taaaggtttt attagaagtt tagttgtaga acatcatcca ttgagtattt gccagcttta
     6421 gttttatcag ccatgtattc acatgctttt aaagcaccta tagcgaaaac atctcttgaa
     6481 atagctttgt gatttatttc tataacttca ccatcaccag caaaaataac ttcatggtct
     6541 cctattattc cgccacctct tactgtattt acacagattt catttttttc tcttttagca
     6601 attccttctc ttccgtaaag taattttgtt tcttcattaa gagaatcttg aatagtatga
     6661 gctaataaaa gggcagttcc acttggagca tcaacttttt gattgtgatg tctttctaca
     6721 agttctatat caaagttttc ataaagaact ggagctactt tctttaataa attatttact
     6781 aaattaatac ctatgctcat gttagcagat ctaaatagag ggatttcttt tgaagattcc
     6841 tctataaatt ttaagtcttc agcagtgtat cctgttgaac atagtataag tggtttttta
     6901 gttttctttg aaaattctac taagcttttt aaagcatcag ctcttgaaaa atctaataat
     6961 acatcatagg aaacattgca atcttctggt tttgcaaaga ttgggtaaga tctttgagcc
     7021 atagtgttat tatctatacc agcaactatt tcaacattag gaaattttgt ttcagctaag
     7081 tttgaaacta cgctacccat ttttcctgaa caaccattta atataacttt aaccataatt
     7141 ggtacctccg atattagttt taaataagca taaccaccta aaggcgatta tgcttattat
     7201 ataatagatt attttattaa accattagct tttaagtttt cttctaatat tactaagtta
     7261 gaatcttcca tttcacataa tggtaatctt aattgtccac agttaaaatt cattaaattc
     7321 atagctgttt taactgggat tggatttgtt tctataaata aagaatttgc taatgaaaga
     7381 tatttaagtt gaaggttacg tgattcaata acttctccta agaaatattt ttcacatata
     7441 ttgtgagttt cttcaggaag aatatttgct aaaactgaaa ccacaccagc tccgcctaga
     7501 gataaaattg gaactatttg atcatcatta cctgaataga tagcaattct atcaccacat
     7561 aattcggcca ttttagctac ttgtgaaaga tctccgctag cttcttttat agcaactaca
     7621 ttatttaaat ttgcaagttt atcaagcatt aatggagtta tattcattcc agttcttgat
     7681 ggaacattat aaagcataat aggaatattt attgcatcat ttatagcact gaaatgagca
     7741 tataatcctt tttgagaagt tttattataa tatggagtta ttattaatac catatcagcg
     7801 ccaacttctt cagcgtattt acttaattct attgaagcct tagtattatt tgttccagtt
     7861 ccagctatta caggaactct tttatttaca acttccacag agaattttat aacttctttc
     7921 ttttcttcta aagtcatagt agaaccttct cctgtagttc cacaaactaa aatagcgtca
     7981 gtatgatttt taatgtgcca ttctaataat tttcttagct cttcataatt aacgccatct
     8041 tcaataaatg gtgttattaa agctacacaa gaccctttaa acatacgtat tacctccaaa
     8101 aaaataaatt atttttcgtt tataagtatt tcagcaattt gaattgcatt aagagctgcc
     8161 ccttttctta tattatcagc cacaacccaa aggttaagac cattatctaa actgaaatca
     8221 cgtcttattc ttccaacaaa gacctcgtct tttccgctaa gttcaagtgg agtagggtat
     8281 tttaagtttt ctacatcatc atgaactatt attcctttag aattattaaa taaatctaca
     8341 acattttcaa gttcaaattc agaattaagc tctacattta tactttcact atgagcattt
     8401 aaaactggta ccctaactgt agtagcagtt attcttaaat tatcatcatg aagtattttc
     8461 tttgtttctt caatcatttt aatttcttct tttgtgtatc cattatctaa gaatacatct
     8521 atatgaggaa ttacattacc taatatagga tatgggaatt tctttgtagt tccttcttgt
     8581 aaatctaata taccttgaat tccagcacct gaaactgctt ggtaagttga gtaaactatt
     8641 ctttttatac catatttatc atatagtggc tttaaagcta ccatagcttg aattgtagaa
     8701 cagtttggat tagcaataat acctttatgc cattttacat cctctggatt tacttccggt
     8761 actactaaag gaacttcttt atccattctc caagcactac tgttatctat aaccactgct
     8821 ccatgctttg cgaaaatagg agcaaactct aagcttacac ttccacctgc tgagaataat
     8881 gcaaaatcta ttttcttgtt ttcaatatta ccttcgcata attcttcaac taatacatct
     8941 tcacctttaa attttaaagt ttttcctgct gatctttttg atgcgaataa atataattct
     9001 ttaacgggga aatttctttc ttctaggatt tcaaggaatt ttcttccaac atttcctgtt
     9061 gccccaacta ttgctacatt gtacacaata tccctcctaa atataatctt aatatattct
     9121 atttctatat attaaatata gttcaatgat aaacacttta tcaagtatat tttatgatta
     9181 atttgaagga tttgagtaag ttaactttca tatttgacta aatatgaata tttaagctta
     9241 taggaggaat aatagtttta aaaataaatt taaggtaggg gaagaatatg agcaagacac
     9301 cattaaaaaa aattataaaa ggaaaaataa aatctaataa ggaattaaca ccagcagaaa
     9361 aattaagaga aaagatgaaa tatgaaatag ctgatgaatt aggactaagt gataaggttg
     9421 ataaatttgg atggggagga ttaactgctg aggaaacagg aagaattggt ggattaatga
     9481 ctaagagaaa aaaagaactt aacttaccta gtaatgatga aatattaggt aggaaaaaac
     9541 ctcacgttga cgaaaagtaa aaaaagtata taatttttag gtagaataat atatggggga
     9601 tttaaattat tatgaagagt tataagatga tggcaaaagt atatgatgaa cttatatatg
     9661 aagatgtaaa ctatgagaac atagcagaat atgccttatc aaaatgtgat aagtataata
     9721 ttaataagga aatgtattta gatttagcct gtggaacagg aaatgttggt gttcatgtag
     9781 gaaaatcctt caaagaaaat tattttgttg atctttcaca agatatgctt atagaagccg
     9841 ataaaaagct tagagaacat agagttagag gtaagatagt ttgccaagac atgtgtgact
     9901 tagggttaaa tagaaagttt gatttaataa catgtgtttt agattcaaca aattatattt
     9961 tagatgatga aagtgtagaa aattatttaa gaggggtata taatcattta aaagaggatg
    10021 gtttatttgt atttgatata aattcatact ataaaatatc agaagtatta ggcaacaatg
    10081 tctacacata tgatagtgaa gaattatttt atgcttggga aaatattttt gaggatgaaa
    10141 tagtggagat gaatttaaat ttctttgtga aagatggaga aaagtatgaa agattcacag
    10201 agattcatga agaaagagct tataaggaaa gtgaaataga acatattctc gatgtaatag
    10261 gttttaaaat actagaaaag cataatggtt atacagaaga tgaagtaaca ccagatactg
    10321 aaagaatagt ttatatagta aaaaaataac ggagggatta aattatgagt gataaattaa
    10381 taagagccat agctaaagat ggcatgataa gaatatttgc aacagaaact acagagttag
    10441 ttaatgaggc ttcaaaaata catgattgca cacctacagc tgctgctgcc ttaggaagaa
    10501 tgttaacagc aggaactatg atgggagcaa tgttaaaatc agataaagaa gttgtaactt
    10561 tacaaataaa tggtggagga atggctaaag gggttactgt aactgcttac tcagattgta
    10621 gtgttaaagg atacatagga aatccacatg ttgatttacc tttaaacaca gaaaatggta
    10681 aattaaatgt tggagaagct attggtaaaa atggtggatt aacagtaatt aaagatttag
    10741 gattaaaaga tccatatgta ggacaagttc ctatatatag tggagaaata gctgaggatt
    10801 tagcatatta ttttactgct tcagagcaaa taccttcagc agttgcccta ggagtattag
    10861 tagatagaga tcattcaata aaaaaagctg gtggatttat aattcaatta ttaccaggag
    10921 ctgatgaact tttaggtgat ttattaacat atagactaga tgagattcct tcattaacta
    10981 caatgctatc agaaggaaaa acaatagaag aagttataga attcattttt gatggaatgg
    11041 atttaaaaat attagaagaa gaaactccaa aatataaatg tgattgctca agagaaaaag
    11101 tagagagagc attattaagc ataggatata aagatttaaa agaattatat gatgaaggta
    11161 aagaagagga attaaaatgc cacttctgta ataaagcata taaatttaca aaagaagata
    11221 taggaaaatt attagaagaa aaagaaaaat ctattgctga tgaagtttca gaagaaatga
    11281 aaaaagcgga agagaaagaa aaaaaagaaa aaaataaaaa ataagttgac aaattaattt
    11341 taagttaata taatagtaaa tgtcttgaag agctcaagac atttggctcg atagctcagt
    11401 cggtag
//
LOCUS       DAEVGI010000073        11345 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEVGI010000073 DAEVGI010000000
VERSION     DAEVGI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.073"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            97..1053
                     /locus_tag="K8M77_001594"
     CDS             97..1053
                     /locus_tag="K8M77_001594"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI7086089.1"
                     /translation="MLSNSPKVTNIDFNQYDIIHFHSTFDLYSVKDTLKDFNGKIVLT
                     SHSPKPYHLEFIEDAINIKDRIKYKEIYEKLDRIDEYAFENTDYIVFPCEDAEEPYFK
                     NWEEYKNIKNKNKNKYKYLLTGCKEGNVCLSRKEICLKYNIPEDAFIISYVGRHNFTK
                     GYEDLKNLGEKILRDNKNIYFLVAGKEEPIKGLKHSNWIEVGWTNDPHSIIAASDIFI
                     LPNKETYFDLVMLEVLSLEKVVLASKTGGNKQFQKYHTKGIILYDNIEDAYLKFNDII
                     LNNKENLSSWGKENRSLFDNKFNSKIFAKKYIELMNNIYNQR"
     gene            1069..2502
                     /locus_tag="K8M77_001595"
     CDS             1069..2502
                     /locus_tag="K8M77_001595"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024832.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HBI7086090.1"
                     /translation="MSLKKNFVYNIIYQIFSLLLPVITVPYISRVLGPNGVGLYSYTL
                     SYAQYFVLIGMIGINLYGNRQIAYSKNNKEKLSKEFWNIYSLQFITTIIAFSMYIIVF
                     IFLNNSNRILYLVQSIVIFTAIFDISWFFMGYEDMSSIVIRNTIVKVIGIISIFLFVK
                     KPSDVIKYAFIIVISNFIGQVIMWFNIKDKIYIYGLSIKESLKHLKPSLALFISQLAI
                     QIYILLDKTMLGIMTNVYEVGLYENSQKTIKLALTLITSLGVVMLPRMSALYSQGKKE
                     EFNNNIYKAFSFVNFMSFPMILGLIAVSNTFSLWFYGNSFYGVGFLLKIGSMLMIFIG
                     WSNILGIQVMLPMKKERQFTISVTIGAIVNFILNLFLIRKFQAVGSTLASVIAEFSVT
                     VVQLYFLKNLIDIKIILKLIVKPLIGAVTMFVILIEIIPLFKVGIGETFIEIAIGFTI
                     YLIVMYILKDSFLQEAFNIMKKKFLKR"
     gene            2513..3616
                     /gene="glf"
                     /locus_tag="K8M77_001596"
     CDS             2513..3616
                     /gene="glf"
                     /locus_tag="K8M77_001596"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HBI7086091.1"
                     /translation="MKKYDYLIVGAGLFGSIFAYEANKRSKKCLVIDKRNHVGGNVYC
                     ENIEGINVHKYGAHIFHTSNKKVWDYINNFVDFNRYTNSPVANYKGELYNLPFNMNTF
                     YQLWGTKTPLEAKNKIEEQKKEAKISEPQNLEQQAISLVGNDIYEKLIKGYTEKQWGK
                     KATDLPAFIIKRLPVRFTFDNNYFNDMYQGIPIGGYNVIIDKLLDGIDLSLETDFFKN
                     REELESLADKIVFTGMIDEFYDYKFGTLEYRSLKFQNEILNEENYQGNAVVNYTEYEI
                     PYTRVIEHKHFEYGTQEKTVITKEYPAKWEKGDEPYYPINNDKNNETYSKYKELADKE
                     DKIIFGGRLAEYKYYDMHNVIERALECVTKEFD"
     gene            3641..4561
                     /gene="galU"
                     /locus_tag="K8M77_001597"
     CDS             3641..4561
                     /gene="galU"
                     /locus_tag="K8M77_001597"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBI7086092.1"
                     /translation="MNKKIRKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIIE
                     EAIASGIEEILIITGRSKKCIEDHFDKSVELEMELEKSGKNELLDLVRDISGMVDIHY
                     IRQKEPRGLGHAIHCAKTFVGDEPFAILLGDDVVYNEEKPCLKQLIDCYNEYKTSVLG
                     VQTVPETHVSKYGIVDGKHIEGKVYKVKGLVEKPSVEEAPSNVAILGRYIVTPRIFDI
                     LENTKPGKGGEIQLTDALLELMGQEAMYAYDFEGRRYDVGDKLGFLEATVEYALRRPE
                     LREGFVEYLNNLSEKESLFKKEVEKEIVTV"
     gene            4972..5619
                     /locus_tag="K8M77_001598"
     CDS             4972..5619
                     /locus_tag="K8M77_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086093.1"
                     /translation="MAEKKQRFINKNVRELRLLGEKGNKEALNELIIRGKKGDKLAIA
                     EIVSIYEGLIHKYSTSFFINGYDVEDLKQVARSVIIESISKFDGDHGSEFIFFISTNV
                     KNKLGTMCKKKENRVDFSSLDSPIGEGITIGETIKDEISIEDEYVERERMRAFVKALA
                     NISKSDRDFILYVYKDRGNFQKYCQEHKHITTYSKARWRKERILKELREEMKNFK"
     gene            5998..8238
                     /locus_tag="K8M77_001599"
     CDS             5998..8238
                     /locus_tag="K8M77_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086094.1"
                     /translation="MNKLKEKGVSLLEPKNKGVFTVNFKHPILKDYKGNGRVITKSLG
                     ADVGKDYEKAKNFTDEIKSIINNSDCYENYYGFLESLEKYSEKAMKIVFDNTHFMEEF
                     YYDSKKNILEKFIEIYHDNINKRLEEELKEVFKLLKLEEFELIDNSNKSFEIFVEEEI
                     RSRFNFEFKKKIQLLGGDYYYRKRLISNLIGSGEKFGKAYKNTNEEKEDKLVNKFFNN
                     MGDPNFEVIFKQDSNKFKLVGTPYSQSIINEKINFKGILEEFLFRINSLDNIFQYKIE
                     FRERKSGKSYVFNNNELLVKRNFIYNKDLDKYPLDNIEDLAYIYFHIGYKENYKQKSQ
                     EELKSKFFYILESLFNEKHNPNSSNLIFSDIRLEGNFRGEILHNKEKDFILADYSGNI
                     NEFINQLLSYETRKNLASSDEVIWLFNIEEEAFERNLEVLKYLIINGYLNKTNIYIYE
                     KTVYKDYFSFEFDNSESDFIDVLNGKEAYNMPNIFNSFLKDNFQNLLEELGEEEPSII
                     IRGREEYYLNLIMDRVIFPCDEEKKVLSNKDVIEKIINQSEEVLINKIKEKSSLINEY
                     KPSYKYERLVGLSLEINRKVKREFIRRVRGSYDKNLKEFSLRMAYNFGGREFKSLCLE
                     KLLEISIKNSIKTFLLNPYKIRKDINILEFEKSIEYIMESISEEIIEEVEILIREDNL
                     LKWKEAAELLKEDIIERRNLIEEIIEETFVVSNDIFREDRVLKIIKEVLKNNSIFKAI
                     KGEFVI"
     gene            8316..9332
                     /gene="galE"
                     /locus_tag="K8M77_001600"
     CDS             8316..9332
                     /gene="galE"
                     /locus_tag="K8M77_001600"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI7086095.1"
                     /translation="MNILLTGGIGYIGSHTIVELLRDDNNSVVVIDNLVNSRKEVKDI
                     VEKITGKSIKFYEGDLVNFEDIDKVFKNEKIDAVIHFASLKAVGESVEKPLEYYNNNI
                     IGTLNLLTSMRNHNVKKFIFSSSATVYGESEIVPVHEGLQAGFATNPYGRCKAMIEDI
                     LRDLYISDNSWDIVLLRYFNPVGAHESGLIGELPNGIPNNLMPYVSKVADGELDYVRV
                     FGDDYNTQDGTGVRDYIHVVDLARGHVAAMKKLKDGSGVNVYNLGTGQGYSVLDIIRA
                     FSKASGKEIPYKIVDRRAGDIAISFADPSKAEKELGWKAEKNLEDMCKDFWKWQCVGM
                     KNFK"
     gene            9372..10316
                     /gene="galU"
                     /locus_tag="K8M77_001601"
     CDS             9372..10316
                     /gene="galU"
                     /locus_tag="K8M77_001601"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBI7086096.1"
                     /translation="MNNKIRKAIIPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYIIE
                     EAIASGIEEILVITGRSKKAIEDHFDRNVELEMELEKSGKTELLDLVRDISDMVDIHY
                     IRQKEPRGLGHAIHCAKTFVGDEPFAILLGDDVVYNEEKPCLKQLIECYNEYRTSVLG
                     VQTVPEENVSKYGIVNGKHIEGRVYKVKGLVEKPSPEEAPSNVAILGRYIVTPRIFDI
                     LENTKPGKGGEIQLTDALLELMREEAMYAYDFEGRRYDVGDKLGFLEATVEYALRRPD
                     LRDGFIDYLRELSATENIFTEANGKEKKDKEEELVTEA"
     gene            10639..11331
                     /locus_tag="K8M77_001602"
     CDS             10639..11331
                     /locus_tag="K8M77_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBI7086097.1"
                     /translation="MIIISQNTFLVVGIILFCLFRGAIKIKGIIKEEYTYKQIYSNEE
                     FIFDLFCIYLIGVASKVYFPLTIAWGKNIVYNFPEIWLSPLWSIKQIYHQGGLGGVIY
                     QLIGNSIMLSPMAFFLCYFYSIDEENLTLGKWIIDKINKILEGFELHNKKKLKEDSYV
                     PSLNTVLKFCFFMSLFIESSQVILSLVFPNTKRFFEVNDLMCNTLSGVWGYYIYIKVI
                     KLDIIPKKIFKL"
BASE COUNT         4576 a          932 c         1996 g         3841 t
ORIGIN      
        1 ataaaaaaaa ataaatatag aaaatattat gataaattac ctaaatattt taaagaagta
       61 tataaatcta aaattagatt aagaaagatt aagtttttgc taagtaatag tcctaaggta
      121 actaatattg attttaatca atatgatatt atacattttc atagtacatt tgatttatat
      181 agtgttaaag atactttaaa agattttaat ggaaaaatag tcttaacatc acattcacct
      241 aaaccatatc atctagagtt tatagaagac gctataaata taaaagatag aataaaatat
      301 aaagaaatat atgaaaaatt agatagaata gatgaatatg catttgaaaa tactgattat
      361 atagtttttc cttgtgaaga tgctgaggaa ccttatttta aaaattggga agaatataag
      421 aacataaaaa ataaaaataa aaataaatat aagtatttat taactggttg taaagaaggt
      481 aatgtatgtt tatcaagaaa agaaatatgt ttaaagtata atataccaga ggatgctttt
      541 ataattagtt atgttggaag gcataatttt acaaaaggat atgaggattt aaaaaattta
      601 ggtgaaaaaa ttttaagaga taataaaaat atatactttt tagttgcagg taaagaagag
      661 cctattaaag gcttaaaaca tagtaattgg attgaggtgg gatggactaa tgatccacat
      721 tctataatag cagcgtcaga tatttttata ttacctaata aagaaactta ttttgattta
      781 gttatgttag aagtattatc tttagaaaaa gttgtattag caagtaaaac tggtggaaat
      841 aaacagtttc aaaagtatca tactaaaggt attatacttt acgataatat tgaagatgct
      901 tatttaaaat ttaatgatat tattttaaat aacaaagaaa atttaagttc ttggggaaag
      961 gagaatagaa gcttatttga taataagttt aattcaaaaa tctttgctaa gaagtatatt
     1021 gaattgatga acaatatata caatcaaaga tagaatttag tatataaaat gagtttgaaa
     1081 aaaaattttg tttataatat aatatatcaa atattttcac tgttactgcc agtaataaca
     1141 gtaccatata tatcaagagt tttaggacca aatggtgtag ggttatattc atatacttta
     1201 tcatatgctc aatattttgt tttaatagga atgattggaa taaatttata tggaaataga
     1261 caaatagctt atagtaaaaa taataaagaa aagttatcaa aggaattttg gaatatatat
     1321 tcattgcaat ttataactac aataatagct ttttctatgt atataattgt ttttatattt
     1381 ttaaataatt caaatagaat tttatattta gtgcagtcaa ttgtaatttt tacagctata
     1441 tttgatatat cttggttttt tatggggtat gaggatatga gtagtatagt tattagaaat
     1501 actatagtaa aagttattgg tataatatca atatttcttt ttgttaagaa accttcagat
     1561 gttatcaagt atgcatttat aattgttatt tctaatttta taggacaagt tataatgtgg
     1621 tttaatataa aagataaaat atatatatat ggattaagta taaaagaatc tttaaaacat
     1681 ttaaagcctt cattagcttt gtttatatct cagttagcaa tacaaattta tatattatta
     1741 gacaaaacta tgcttggtat tatgactaat gtttatgaag ttggattgta tgaaaactca
     1801 caaaaaacaa taaaattagc tttaacatta ataacatcat taggggttgt aatgttacct
     1861 agaatgagtg ctttatattc tcaagggaaa aaagaagaat tcaacaataa tatatataag
     1921 gcttttagtt ttgttaattt tatgtcattc cctatgattt taggtcttat agcagtatct
     1981 aatacttttt cattatggtt ttatggaaat tcattttatg gagttggatt tttattaaag
     2041 atagggtcta tgttaatgat ttttatcgga tggagtaata tattaggtat tcaggttatg
     2101 cttccaatga aaaaagaaag acagtttact atatctgtga ctattggagc tatagtaaat
     2161 tttatattaa atttattttt aataagaaaa tttcaagctg ttggaagtac tttagcttca
     2221 gtaatagctg agttttcggt aactgttgta cagttatatt ttttgaaaaa tttaatagat
     2281 attaaaatta ttttaaaatt aatagttaaa ccattaattg gagcggtaac aatgtttgta
     2341 attttgatag aaataatacc attgtttaag gtgggaattg gagaaacatt tattgaaatt
     2401 gctatagggt ttactattta tttaatagta atgtatattt taaaagactc atttttacaa
     2461 gaagcattta atataatgaa aaagaaattt ctaaaaagat aggagaatat ttatgaaaaa
     2521 atatgattat ttaatagttg gagcaggtct ttttggatca atatttgcat atgaagcaaa
     2581 taaaagaagc aagaaatgtc tagtaataga taaaagaaat catgttggtg gtaatgttta
     2641 ttgtgaaaat atagaaggaa taaatgttca taaatatggt gctcatatat ttcatacaag
     2701 taataaaaaa gtttgggatt atataaataa ttttgtagac tttaatagat atacaaattc
     2761 accagtagca aattataaag gagaattata taacttacct tttaatatga atacattcta
     2821 ccaattatgg ggtactaaga ctcctttaga agctaagaat aagatagagg aacaaaagaa
     2881 agaagctaaa atatctgaac cccaaaattt agaacaacaa gctataagtt tggttggaaa
     2941 tgatatatat gaaaaattga taaaaggtta tacagaaaag caatggggta aaaaagcaac
     3001 tgatttacct gcatttataa taaaaagatt accagtaagg tttacttttg ataataatta
     3061 ttttaatgac atgtaccaag gaattcctat tggtggatat aatgttataa tagataagct
     3121 attagatggc atagacttaa gtttagaaac agattttttc aaaaatagag aagaattaga
     3181 aagtttagct gataaaatag tatttacagg catgatagat gaattctatg attataaatt
     3241 tggaacttta gaatatagaa gtttaaaatt tcaaaatgaa atattaaatg aagaaaatta
     3301 tcaaggaaac gctgttgtga attatactga atacgaaatt ccatatacaa gagtcataga
     3361 gcataaacat tttgaatatg gaacacaaga aaagacggtt ataactaaag aataccctgc
     3421 aaaatgggaa aaaggtgatg aaccatatta tccaataaat aatgataaaa ataatgaaac
     3481 atattctaaa tataaagaat tagctgataa agaggataag ataatatttg gtggtagact
     3541 agctgagtat aaatattatg atatgcataa tgttatagaa agagctttag aatgtgtaac
     3601 aaaagagttt gattaacata tggtttaagg ggataaaaaa gtgaataaga aaataagaaa
     3661 agctataata ccagcagcag gtcttggtac tagattttta cctgcaacaa aggcacaacc
     3721 taaggagatg cttccaatag ttgataagcc aactattcaa tacataattg aggaagctat
     3781 agcctcaggg attgaagaga ttcttattat tacaggaaga agcaaaaaat gtattgagga
     3841 tcactttgat aagtctgttg agcttgaaat ggagcttgag aaatcaggta agaatgagct
     3901 tttagatttg gttagggata tatcaggtat ggttgacatt cactatataa gacaaaagga
     3961 gccaagagga cttggacatg ctatacattg tgcaaagact tttgtagggg atgaaccttt
     4021 tgctatacta cttggggatg atgtagttta taatgaggaa aaaccatgtt taaaacagtt
     4081 aattgattgt tataatgagt ataaaactag tgtattagga gttcaaactg ttccagaaac
     4141 tcatgtttct aagtatggaa tagtagatgg aaaacatatt gaaggaaaag tttataaggt
     4201 taagggattg gttgaaaaac catctgttga agaagcacct tcaaatgttg ctatattagg
     4261 aagatacata gtaactccaa gaatatttga tattttagaa aatactaagc ctggaaaggg
     4321 tggagaaatt caattaacgg atgctctttt agaacttatg ggacaagaag caatgtatgc
     4381 ttatgacttt gagggaagaa gatatgatgt tggagataaa cttggattct tagaagctac
     4441 tgttgagtat gctttaagaa gaccagagct tagagaagga tttgttgaat atctaaataa
     4501 tttatcagaa aaagaatctt tatttaaaaa agaagttgaa aaagagattg ttactgtata
     4561 attttcatta ataaaatcca gtagagagtt gatttaaatt ctctactgga ttttattgat
     4621 ttttttgttt atattctaca cagatatttt tttgatgttt attttttatg attaagtttc
     4681 attatattaa gccaatattt aaagaaactt aacataataa tacagctttt tctcctacta
     4741 gagcatattt aaatagtgaa gaggttttaa tatgtattta ttgttaaaaa atatagaata
     4801 atttgttatt ctgttgaaat gtaaatgttt aaatggtaag ataaatttac aataaataac
     4861 ataactttat acattattaa aaataaaact gtttaattta aaattaaaaa cataactagg
     4921 tatattaata ggtgatttaa aaattctgca ttttaagggg gataattagg tatggcagaa
     4981 aagaaacaaa gatttattaa taagaatgtt agggaactta gacttttagg agaaaagggg
     5041 aataaggaag ctttaaatga gttgattatt agagggaaaa aaggtgataa acttgccata
     5101 gctgagattg tttctattta tgaaggatta attcataaat actctactag tttttttatt
     5161 aatggttatg atgttgagga tttaaagcaa gtagctagaa gtgttattat tgaatccatc
     5221 agtaaatttg atggggatca tggctctgaa tttatatttt ttattagtac aaatgttaag
     5281 aataaacttg gaaccatgtg taagaagaag gaaaatagag ttgatttttc aagcttagat
     5341 agtcctatag gggaaggtat tactattggt gagaccatta aggatgaaat atctatagag
     5401 gatgaatatg ttgagagaga aagaatgaga gcttttgtaa aagctttagc taatatttca
     5461 aaaagtgata gggattttat actttatgta tacaaagata gagggaattt tcaaaaatat
     5521 tgccaagagc ataagcatat tacaacctat tcaaaagcaa gatggagaaa ggaaagaata
     5581 ctaaaagaac taagagaaga aatgaaaaac tttaaataat agacttaact ataaaaatca
     5641 aaggaaaaat tataggagaa acttaatatg gataagattt ttaaaaatta ttaaaatagg
     5701 caatatcttt ttttagaaga gaattgtcta ttttttataa atagagtaaa gaaataattt
     5761 atattaatag ttttttgtag tttaatctaa aatttatatt ggttcaaaat aggggatact
     5821 taaatctata agcttttaat ttattattaa tattaaattc tgagctcaat atgagcgcat
     5881 aaattcatta taattttaat tagaaattag tttatatctt aatttaaata tttatttaca
     5941 ttttagttaa gtattaaggt attagctatt tttatttgga ttatggaagg tgagtttatg
     6001 aataagctaa aagaaaaggg agtctcatta ctagagccaa agaataaggg ggtttttaca
     6061 gtaaatttta agcatccaat tttaaaggat tacaaaggga atggaagagt tataactaaa
     6121 agtttaggag ctgatgtagg gaaggattat gaaaaagcta agaattttac tgatgaaatc
     6181 aagagtatta taaacaatag cgactgttat gaaaattatt acggcttttt agaaagttta
     6241 gaaaagtata gtgaaaaagc tatgaaaatt gtttttgata acactcattt tatggaggag
     6301 ttttattatg attctaaaaa aaatatttta gaaaaattta ttgaaattta tcatgataat
     6361 attaataaga gattagaaga ggaattaaaa gaggttttta aacttcttaa actagaagaa
     6421 tttgaattaa tagataatag caataaatcc tttgaaattt ttgtggaaga ggagattagg
     6481 tctagattta actttgaatt taaaaagaaa attcagttgt taggtgggga ttattactat
     6541 agaaagagat taatttctaa tcttattgga tcaggtgaaa agtttggtaa agcgtataaa
     6601 aatactaatg aagaaaaaga agataaattg gtaaataagt tttttaataa tatgggagat
     6661 ccaaattttg aagtgatttt taaacaggat tctaataagt ttaaacttgt tggaactcca
     6721 tattcacaat caattattaa tgaaaaaata aattttaagg gaattttgga agagttttta
     6781 tttaggataa atagtttgga taatattttt caatataaga ttgagtttag ggaaagaaaa
     6841 agtggaaaaa gttatgtttt taataacaat gagttattag ttaagagaaa ctttatttat
     6901 aataaggatt tagataaata tcctttagat aatattgaag atttagctta tatttatttt
     6961 catataggtt ataaggagaa ttataaacaa aagtctcaag aggaattaaa gtcaaagttt
     7021 ttctatatat tagagagtct ttttaatgag aagcataatc ctaatagcag caatttaata
     7081 tttagtgata taagattaga aggaaatttt aggggagaaa ttttacataa taaagagaag
     7141 gattttattt tagctgatta tagtggaaat attaatgagt ttataaatca attattaagt
     7201 tatgagacta ggaaaaattt agctagtagt gatgaggtaa tatggctttt taatattgaa
     7261 gaagaggctt ttgaaagaaa tttagaggtt ttaaaatact taattataaa tggatattta
     7321 aataaaacta atatatatat ttatgaaaaa actgtttata aggattattt ttcttttgaa
     7381 tttgacaata gtgaaagtga ttttattgat gtattaaatg gaaaagaggc atataatatg
     7441 cctaatattt ttaattcttt tttgaaagat aactttcaaa atttattgga ggaattagga
     7501 gaggaagagc cttcaataat aattagggga agagaagagt attatttaaa tttaataatg
     7561 gatagagtaa tatttccttg tgatgaagag aaaaaggtat taagtaataa ggatgtaata
     7621 gaaaaaataa taaatcaaag tgaagaagtt ttaataaata aaataaaaga aaaaagttcc
     7681 ttaataaatg agtataagcc atcttataaa tatgaaagat tagtaggctt atccttagaa
     7741 attaatagga aagttaagag agaatttata agaagagtaa gaggaagcta tgataagaat
     7801 cttaaagagt tttccttaag aatggcttat aactttggag gaagagaatt taaaagccta
     7861 tgcctagaga aattattaga aatctcaata aaaaacagta taaagacctt tttattaaac
     7921 ccatacaaaa taagaaagga tataaatatt ttagaatttg aaaagtccat agaatatatt
     7981 atggaaagta taagtgagga aataatagaa gaagttgaga ttttaataag agaagataac
     8041 ttattgaaat ggaaggaagc agcagagctt ttaaaagagg atattataga gagaagaaat
     8101 ttaatagaag agataataga agagacattt gtagtaagta atgatatttt tagagaagat
     8161 agagtattaa aaataataaa agaggtatta aaaaataata gtatatttaa agcaataaag
     8221 ggggaatttg ttatataaat agtatataat ttgaaatttt taataaattt atgctataat
     8281 ttaagctgta attttttggt gagggagaag tttaaatgaa tatattatta actggtggaa
     8341 ttggctatat aggaagccat acaatagtag aattattaag ggatgataat aattctgtag
     8401 ttgttattga taacttagta aatagtagaa aagaagttaa ggatatagtt gaaaaaataa
     8461 caggaaaaag tataaaattt tacgaaggtg atttagtaaa ttttgaagat atagataagg
     8521 tttttaaaaa tgaaaaaata gatgctgtta tacactttgc ttctcttaag gcagtagggg
     8581 aatcagtaga aaaaccttta gaatattata ataacaatat tattgggact ttaaatcttt
     8641 taacaagtat gagaaatcat aatgttaaga aatttatatt tagttcttca gctactgttt
     8701 atggagaaag tgaaatagta cctgtacatg aagggcttca agcaggtttt gcaacaaatc
     8761 catatggtag atgtaaggct atgatagagg atattttaag agatttatat atatcagata
     8821 attcatggga tattgtactt cttagatact ttaatccagt tggagctcat gaaagtggat
     8881 taattggaga attacctaat ggaataccaa ataaccttat gccttatgtt tcaaaggtgg
     8941 ctgatggtga attagactat gtaagagtat ttggagatga ttataatacg caagatggaa
     9001 ctggtgtaag ggactatata catgttgtag acttagcaag aggacatgta gcagctatga
     9061 aaaagttaaa ggatggttct ggagttaatg tatataatct aggaactgga caaggatatt
     9121 ctgtactaga tataataaga gctttttcaa aggcatcagg taaggaaata ccttataaaa
     9181 tagtagatag aagagcagga gatattgcca taagttttgc agatccatca aaggcagaaa
     9241 aagaacttgg atggaaggct gagaaaaatt tagaggacat gtgcaaagac ttctggaaat
     9301 ggcaatgcgt tggaatgaaa aactttaaat aaacttcata ttaaaatttt gatttacaag
     9361 gggataagag gatgaacaat aaaataagaa aagctataat accagcagca gggcttggta
     9421 ctagattctt acctgcaaca aaggctcagc ctaaggagat gcttccaata gttgataaac
     9481 caactattca atacataatt gaggaggcta tagcttcagg tatagaggaa atacttgtta
     9541 ttacaggaag aagtaaaaag gctattgaag atcactttga ccgtaatgtg gaacttgaaa
     9601 tggaacttga aaagtcagga aaaactgaac ttttagattt agttagagat atatcagata
     9661 tggttgatat ccattatata agacaaaaag aaccaagagg acttggacat gctatacatt
     9721 gtgctaagac tttcgtaggg gatgaacctt ttgccatatt acttggtgat gatgtagttt
     9781 ataatgaaga aaagccatgt ttaaagcaat taattgagtg ttataatgaa tatagaacta
     9841 gtgtattagg ggttcaaact gttccagagg aaaatgtttc taaatacgga atagttaatg
     9901 gaaaacacat tgaaggtaga gtttataaag taaaaggctt agttgaaaag ccatcaccag
     9961 aagaagcacc ttcaaatgtt gctatattag gaagatatat agtaactcca agaatatttg
    10021 atatattaga aaacacaaaa cctggtaaag gtggagaaat tcaattaaca gatgcacttt
    10081 tagagcttat gagggaagaa gctatgtatg cttatgactt tgaaggaaga agatatgatg
    10141 tgggagataa attaggattc ttagaagcta ctgttgagta tgctttaaga agaccagatc
    10201 ttagagatgg gtttattgat tacttaagag aattatctgc tacagaaaat atctttactg
    10261 aagcaaatgg taaagaaaaa aaggataaag aagaagagct tgtaacagaa gcttaatttt
    10321 agaaggctgt atcaaaataa ctcataaata aacttaaaaa ccctaattaa tttcatttat
    10381 tttttagtta ggtttgtaag agttttaagg agttttgata cagcttattt ttataattaa
    10441 tttgattaaa ataaaaataa agaggctata cttaataaag taatataaag ctatttattt
    10501 tatgaggttt tgatttctaa agaatataag attatattaa tgaataatag aagttttttt
    10561 agtattaata ctgtatttag aagtcaacat taacttaaat aatatattaa ttaaaattta
    10621 gaattaaaag gagaatttat gattattatt tcccaaaata catttttagt agtaggaatt
    10681 atactctttt gtttatttag aggagcaatt aaaattaaag gaataattaa ggaagaatac
    10741 acatataagc aaatatattc taatgaagaa tttatctttg atttattttg tatttactta
    10801 attggagttg cctctaaagt ttactttcct cttaccatag cttggggaaa gaatattgtc
    10861 tataattttc cagaaatttg gcttagtcct ttgtggagca taaagcaaat ataccatcaa
    10921 ggaggattgg gaggagttat ttatcagcta ataggaaatt caattatgtt aagtccaatg
    10981 gcgttttttc tttgctattt ttatagtata gatgaagaaa atttaacttt aggaaaatgg
    11041 attatagata aaatcaataa aattttagaa ggctttgaat tacataataa aaagaagctt
    11101 aaagaggatt catatgtacc ttcattaaat acagtgttga aattctgctt ttttatgtct
    11161 ttattcatag agagttccca ggttatatta tccttagttt ttcctaatac aaaaaggttt
    11221 tttgaagtaa atgatcttat gtgtaatact ttaagtggag tttggggata ttatatttat
    11281 attaaagtta ttaaattaga tataatacca aaaaaaatat ttaagttata aaataaaaag
    11341 taatt
//
LOCUS       DAEVGI010000074        11286 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEVGI010000074 DAEVGI010000000
VERSION     DAEVGI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.074"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            75..1526
                     /gene="cls"
                     /locus_tag="K8M77_001603"
     CDS             75..1526
                     /gene="cls"
                     /locus_tag="K8M77_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI7086098.1"
                     /translation="MIFTFFSNLVHIVNLLTIFYMIFKENRSSKSIISWTLVLFILPY
                     IGLILFLMLGRKVNNKDIFIMKKSELTLFDRHIKKLNNNSNDKFKISNHSDMIKAIES
                     MEYSPYRKDVETKVFFDGKELFNDILESLKKAQKSINIEFYIFKNDDIGSKILDILKE
                     KAKAGVEVRLLYDSVGSRTTNRKKLQSVIDAGVKVGEFFPSLLRLININMNFRNHRKI
                     IVIDNKIGYVGGFNVGDEYLGKDPKFGYWRDTHIKFMGDSVRDLDLRFWADWKYATKE
                     DIDLIQLMDYKNEQAPSKMANYSKCGMQIISSGPNPDNFYEIKLSYLEMIQKAKKYIY
                     IQSPYLILDDSLTDSLKLASISGVDVRIMIPGKGDHPFVYWANLKYAGDLLNFGVKIY
                     HYDKNAFLHSKTLVIDDEVCSIGTANMDTRSFELNFEVIAMLYSDEIAKIQRKQFEKD
                     MLISKELTREEYLKRGTSVKIKESFSKLFSALL"
     gene            complement(1628..1966)
                     /locus_tag="K8M77_001604"
     CDS             complement(1628..1966)
                     /locus_tag="K8M77_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBI7086099.1"
                     /translation="MENSEKLSRKEKRELVENNLLARVYDLILNEQTSEEERSILVEF
                     KNAVESGKDFQLETMKLAETLRQLALSKFKHKEKLSNEVSKFYMDISTTGLFEKNLAI
                     GLCATKFWFQ"
     gene            complement(2374..5772)
                     /locus_tag="K8M77_001605"
     CDS             complement(2374..5772)
                     /locus_tag="K8M77_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBI7086100.1"
                     /translation="MKAYKKSIVREILSSKARFFSIMVIIFLGVAFYSGIKSAGPDMN
                     KTINKFYNSENLMDSKVISTLGLSDKDLDLLKNDPKILDYYGTHSIDANLTNMSNVVK
                     FMEYDGKNNINNLVVLEGKLPEKSGEIALDERAFKLNKNLKIGDNFIIETDENTMKNF
                     KESKFKIVGIVKSPMYIEKESRGSTTVGKGTIDYFGILNSNDINMDAYTEIYVRFKNV
                     QGLDAYGDEYKELMEENNKYLEDLYSNREVERVEEIKREAKKEFNKAYEELNENEKLL
                     LDKEKELQEGKAQLAEGKKQYSDKKLEFEKGIKEGEANILKGEKQLEEGQAEIKNQKS
                     NMEEGETKLNDVKTTLHNSRQEFLNQGINPDESTNELKDKIENLKNLSKTMTFLSNEI
                     KTTVTNLSENEKIPAEKIQYWKVIVANLGLNELNGAIEELEKNPENKNIALGLSDSLD
                     KSVNSINDNTSKLQVLLDGITKYQEGKNQYETQVVLFNEGKSKLYEAEKQLEKGKVEL
                     AEGKKSLEEGKAKGERALQKAKKKLDESEEKLKEGQKAIEENKEKLTEGRKEVEEKEK
                     STLNDLNECNYYVFDRTDNPGYSGYKDSINSLDSIASVLPVFFFIVAVLICLTTMTRM
                     VEEKRIEIGTMKALGYGDFEISLKFVIYAALASILGCLLGILVGSNILPKIISNAYTS
                     VYALPSIDTYYYPSYIIQALVISILCTVGAALFVVRVELKNKPSNLMRPKAPKLGKKI
                     LLERITPIWKRLNFNQKVTFRNIFRYKQRMLMTIFGIAGCMALLVTGFGLKDSNTGMV
                     EKQFDKLWKYETMVVFGDNPTKTEIEKYNETLKELPGYENNLRMHQESVTFSKEGMNK
                     QTVSLYVPENKDELDKFIVLNDRISGEKYEIPDEGVVINEKLGKLLGVSIGDTITFKD
                     ENNNPHEVKVEEIAENYLMHSMYMSSSYYEKVFGKKANYNTDFINFDKSKINEDEISS
                     KLMECKDVVNVTMTSQIEKTTKESAANMNVVMLVIILSAGSLAFVVLYNLNNINVSER
                     IRELSTIKVLGFFDDEVTMYILRENIILTLLGILAGSVLGKVLHAFIIRTSETDTMMM
                     YPKIHISSYIFSALITILFTVIVMILMHVKLRKVNMIDALKSNE"
     gene            complement(5789..6490)
                     /locus_tag="K8M77_001606"
     CDS             complement(5789..6490)
                     /locus_tag="K8M77_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086101.1"
                     /translation="MAYIDVIDVHKRYKVGENIVVANNGINFSIDKGEFVVILGPSGA
                     GKSTTLNILGGMDSCDEGKIIIDETDISKFSNRELTKYRRYDVGFVFQFYNLVQNLTA
                     KENVELATQISNNALDVEKTLELVGLGHRKDNFPAQLSGGEQQRVSIARAIAKNPKLL
                     LCDEPTGALDYSTGKQILKILQDTCRKIGTTVIIITHNSAIAPMADKVIKINDAKVRS
                     IEINSNPISVEEIEW"
     gene            6888..7442
                     /locus_tag="K8M77_001607"
     CDS             6888..7442
                     /locus_tag="K8M77_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI7086102.1"
                     /translation="MAGVSGNRRTLYTKKVIKESLIELLKTREIHQVTVTDICKKADI
                     NRGTFYTHYKDAFDLFQSMEDELFHQILKYIKEIPIEEYNSLLLLKVLKLIKENKDLC
                     KVLFCNQRDSSILNRILNIADQIDVMKLFNNHNLINKTIANYYMRYSVGGCISIIETW
                     LENDLPESPEELVQIINGINQMSS"
     gene            complement(7530..7832)
                     /locus_tag="K8M77_001608"
     CDS             complement(7530..7832)
                     /locus_tag="K8M77_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086103.1"
                     /translation="MSLEKNFEKELIKKCEIAEKEYGCPQKRLLLTIEKFGGVRTVQE
                     ILRKGRVSDGFDKLQKANRLDLTMEATIVESKYNELFTDEEVNSCHELLCEYGYYR"
     gene            complement(7976..9427)
                     /locus_tag="K8M77_001609"
     CDS             complement(7976..9427)
                     /locus_tag="K8M77_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBI7086104.1"
                     /translation="MISFLLSLVLLIVGYFTYGKFVDKTFGTSDSIETPAESMEDGVD
                     YVPMSWPRIFLIQFLNIAGLGPIFGAISGALWGPVAFLWIVFGCIFAGAVHDYFTGVL
                     SIRNKGVSVAELVGKYLGEIPRKIMVIFSVVLLILVGVVFLTGPADLLKTLTGIDRDI
                     FIVIIVVYYIAATVLPVDKVIGKIYPIFGLCLLIMAIGIGAGIVIEGYNIPEIQLSNF
                     HPEGTPIFPFLFITIACGAISGFHATQSPIMARCVKKESEARKVFYGAMIAEGVIALV
                     WAAAAMSFFGDTNGLQEILKAGGPATVVNTISNTVMGKVGAILAILGVVACPISSGDT
                     AFRSARLTIADAFKLKQDKVIDRFKIAIPLFVVGIALTFIDFSIIWRYFSWSNQTLAM
                     IMLWAASAYMLKAHNNYWITLVPAVFMSAVTCSYILQAPEGFRLNATFSNIAGVVFAL
                     VLFGIFIKYCKKESNENCEVIEVEAVEEVIKNK"
     gene            complement(9770..>11286)
                     /locus_tag="K8M77_001610"
     CDS             complement(9770..>11286)
                     /locus_tag="K8M77_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HBI7086105.1"
                     /translation="KKVLSDRFDEEVFSKNNSNLVLVVNSINDLIRLECNKLKEDEDN
                     LNLIIKRFVRLIEIAHKNRARIIFTTIPPFSGENKNLEYIRNEINSWIRKSTFLDGYL
                     DLDKIVEKRLGVSKDKKEINYDKELEEYMVENISLYYIVERLKPFELDHMSQSDLIKA
                     MNENARFINEDGVNILVKPIPDPVEGTRIDRRIKYFDEYKRPEKSGNPYVFNGEAVGD
                     MRDNMGLLNLNLCKSNILMSKENINGVNCRVYKKEGLKENLPCIVYIHGGAFIGGSLD
                     VSENPCKLIAEGINGIVISVDYSLAPEKPYPLGLNDCRKVVEYIEENNFFYGIDKNKI
                     GIVGESAGANLSTIVANENSNIKFQGLVYPVVTFVEKNPFFNWDIDLYENPYKEEKIY
                     NFINSLRNCEDLVQKLYIQRELDPRREDLSPIFNKNLSKAKKTLIAVSEYDYLRVQGE
                     AYGKLIHKAGVETKIIRYEGVNHAFLDNLGIYPQAEDTINEIVKEFIDTIGNKF"
BASE COUNT         3768 a         1914 c         1212 g         4392 t
ORIGIN      
        1 gattcaattc aaaaataaaa taactaaaat gaatttataa aataataaga acatatagaa
       61 aggatttatt aattatgatt tttacttttt tctctaattt agttcatatt gtaaatttat
      121 taactatttt ttatatgatt tttaaagaaa atcgttcttc taaaagtata atttcttgga
      181 cattagtttt atttatatta ccatacatag gtcttattct atttttaatg ttaggaagaa
      241 aggttaataa taaagatatt tttatcatga aaaaatctga acttacctta tttgatagac
      301 atataaaaaa attaaataac aattcaaatg ataagtttaa aatatctaat cattctgata
      361 tgattaaagc tattgaaagt atggaatact caccttatag aaaagatgtt gagactaaag
      421 tattttttga tggcaaagaa ttatttaatg atattttaga aagcttaaag aaagctcaaa
      481 aaagtataaa tatagaattt tacatattta aaaatgatga tattggttca aaaatcttag
      541 atattcttaa agaaaaggct aaagctggcg ttgaagtaag attattatat gactctgtag
      601 gaagtagaac taccaataga aaaaaacttc aatctgtaat agatgctggt gtaaaagttg
      661 gtgaattctt cccttcccta cttagattaa taaacataaa catgaacttt agaaatcatc
      721 gtaaaataat cgttatagat aataaaatag gctatgttgg cggctttaac gttggagatg
      781 aatatttagg taaagatcct aagtttggtt actggagaga tactcatata aaattcatgg
      841 gtgactcagt aagagactta gatttaaggt tttgggctga ttggaaatac gctacaaaag
      901 aagatattga tttaatccaa ttaatggatt ataaaaatga acaagcccct tctaaaatgg
      961 ctaattattc aaaatgtggt atgcaaataa tatctagtgg acctaaccct gataattttt
     1021 atgaaattaa attatcatat ttagaaatga ttcaaaaagc aaaaaaatat atttatatac
     1081 aatcacctta cttaatttta gatgatagtc taactgatag tttaaaactt gcatctattt
     1141 ctggtgttga tgtaagaata atgatacctg gtaaaggtga ccatcccttt gtttattggg
     1201 caaatctaaa atacgctgga gatttactaa actttggagt aaaaatatat cattatgaca
     1261 agaatgcttt ccttcactct aaaaccttag ttatagatga tgaagtatgt tcaataggaa
     1321 ctgccaatat ggatacaaga agctttgaac taaactttga agtaattgct atgctatact
     1381 cagatgaaat agcaaaaata caacgtaaac agtttgaaaa ggacatgctt atttcaaaag
     1441 aattgactag ggaagagtat cttaagagag gaacttcagt gaaaattaaa gaatcatttt
     1501 caaaactctt ctcagcttta ctttaatatt aaataaattt actcccttta ctttttatat
     1561 ctttctatat aataagatat aaaaagtaaa gggagtttaa acataactat acttccaatg
     1621 aaaaccttta ttgaaaccaa aatttagttg cacataaacc aatagctaaa tttttctcaa
     1681 atagtcctgt agtagaaatg tccatataaa acttactaac ttcattacta agtttttctt
     1741 tatgtttaaa cttactaagt gctaattgtc ttaaagtttc tgctaatttc attgtttcta
     1801 attgaaaatc ttttccactt tcaactgcat ttttaaattc aactaagata ctacgttctt
     1861 cttcagaagt ttgttcattt agaattaaat cataaactcg tgctaataaa ttattttcta
     1921 ctaattctct tttttctttt ctgcttaatt tctctgaatt ttccataaaa atacctccta
     1981 aaactcctta gaatattgca tccataatgc aatctccttg acatagtttt tttatactca
     2041 taaaaaatca agatattata aaaataatca gcatatttat cataattgct gattattttt
     2101 ttatatttat ttatcaatat ctattttatt tgttaataaa atcaaaatac ttaacagtat
     2161 aattttaaca ttaatttcat atttttagaa gtcttgcgtt tttgcacatt tattgtatta
     2221 acttttaaat tttaactata ctattcaaaa cttttaccac ttaattatta atatgtccta
     2281 tttatattca gtcaaccatt aataaaactt acaaacatat taatactatt tgtaagtttt
     2341 atttttatac cttaatacat cccattttct tttttattca tttgatttaa gtgcatcaat
     2401 catattaact tttcttaact taacatgcat aagtatcata actattactg tgaaaagtat
     2461 agttattaaa gctgagaata tataacttga tatatgtatc ttaggataca tcatcatagt
     2521 atctgtttct gatgtcctta ttatgaaggc atgtagaacc tttcctaaaa ctgatcctgc
     2581 caatattcct aataaggtta agattatatt ttcccttaat atatacattg taacttcatc
     2641 atcaaagaat ccaagaactt ttatggttga tagttctctt atacgctcag atacatttat
     2701 attgtttaga ttgtataaaa ctacaaaagc taatgatcct gctgataata ttattactag
     2761 cattactaca ttcatgttag ccgctgactc tttagttgtc ttttctattt gtgatgtcat
     2821 tgttacattc acaacgtcct tgcattccat aagttttgag gatatttcat cttcattgat
     2881 cttactcttg tcaaagttta taaaatctgt attataatta gccttcttcc caaagacttt
     2941 ttcataatat gatgatgaca tatacattga atgcataaga taattttcag ctatctcctc
     3001 tactttcacc tcatgaggat tattattttc atccttaaag gttattgtat ctccaatgga
     3061 tacccctaaa agttttccta acttttcatt aataacaacg ccctcatctg gaatttcata
     3121 tttttctcct gaaattctat catttaaaac tataaactta tctaactcat ctttattttc
     3181 aggtacatat agactaactg tttgcttatt cattccttct ttgctaaagg ttactgattc
     3241 ttggtgcatt cttaaattat tttcatatcc aggtaattct tttaaagttt cattatattt
     3301 ttcaatctct gtttttgtag gattgtctcc aaagactacc atagtttcgt atttccataa
     3361 cttatcaaat tgcttttcaa ccattccagt atttgaatcc ttaagtccaa agccagtaac
     3421 aagaagtgcc atacatcctg caatgccaaa gatagtcata agcatacgct gtttatatct
     3481 aaatatattt ctaaatgtta ccttttgatt aaagtttaat cttttccaaa ttggtgtaat
     3541 tctttctaat aatatctttt tgcctagttt tggagccttt ggtctcataa gatttgatgg
     3601 cttattttta agctcaactc ttacaacaaa taaggctgct cccactgtac ataatattga
     3661 tattactaaa gcttgaatta tataagatgg atagtaataa gtatctatac taggaagagc
     3721 atatacactt gtataagcat tgcttattat tttaggaaga atgttgcttc caactaatat
     3781 tccaagtaga catcctaaaa tacttgctaa ggctgcatat attacaaact ttaaggaaat
     3841 ttcaaaatct ccatatccta aagccttcat agtacctatt tctattctct tctcctctac
     3901 cattcttgtc attgttgtta aacaaattaa gactgccact ataaagaaaa acacaggtaa
     3961 tactgatgca atgctatcta aactattaat agaatccttg tatcctgaat atcctggatt
     4021 atcagttcta tcaaatacgt agtaattaca ttcattcaaa tcatttaaag ttgatttttc
     4081 tttttcttca acttcttttc ttccttctgt tagtttttcc ttattttctt ctattgcttt
     4141 ttggccctct tttaactttt cttctgattc atctaacttc ttctttgcct tttgaagcgc
     4201 tctttctcct ttagcttttc cttcctcaag agattttttt ccctcagcta attctacctt
     4261 acccttttct aattgttttt cagcttcata aagtttactt ttaccttcat taaataaaac
     4321 cacttgagtt tcatactgat tttttccttc ttgatattta gttattccat ctaacaatac
     4381 ttgtaatttt gaagtattgt cattaataga gttaacgctt ttatctagag aatcagaaag
     4441 tcctaaagca atatttttat tttctggatt tttttctaat tcctctatcg ctccatttaa
     4501 ctcatttaat cctaagttag ctactatgac tttccagtac tgaatctttt cagctggtat
     4561 tttttcattt tctgaaagat tagtaactgt agtttttatc tcattagata ggaaagtcat
     4621 tgttttagat aaatttttta gattttctat tttatccttt aattcatttg tactttcatc
     4681 tggattaatt ccctgattta aaaattcttg tctactatta tgaagggtag tttttacatc
     4741 atttaacttt gtttctccct cttccatatt agacttttga tttttaattt cagcctgccc
     4801 ttcttctaat tgcttttcac cctttagaat attggcttct ccttctttaa ttcccttttc
     4861 aaactctagc tttttatcac tatattgttt tttcccttct gctaattgtg cttttccctc
     4921 ttgtaattct ttttccttat ctaaaagtaa cttttcattt tcatttaact cttcataagc
     4981 cttattaaac tcttttttgg cttccctttt tatctcttca actctttcta cttctctatt
     5041 agaatataaa tcttcaagat atttattatt ctcttccatt aattccttgt actcatctcc
     5101 ataagcatca agaccttgta cattcttaaa tcttacatat atttcagtgt aagcatccat
     5161 atttatatca ttgctattta atatgccaaa gtaatcaata gttccttttc caacagtagt
     5221 tgatcctctt gactcttttt caatatacat tggacttttt acaatcccaa cgattttaaa
     5281 cttactttct ttaaagttct tcattgtatt ttcatcagtc tcaattataa aattatcacc
     5341 aatttttaag tttttattaa gcttaaaagc tctttcatct aaagctattt caccactttt
     5401 ttctggtaac ttaccttcta atacaactaa attatttata ttattttttc catcatactc
     5461 cataaactta accacattgc tcatattagt taagtttgca tctatactat gagtaccata
     5521 ataatctaaa atcttaggat cattcttaag taaatccaaa tctttatcac ttaatcctaa
     5581 agttgaaata accttgctat ccataagatt ttcactatta taaaacttat ttatggtttt
     5641 attcatatct gggcctgctg attttattcc agagtaaaag gcaaccccta aaaatataat
     5701 taccattata gaaaagaatc tggcttttga tgatagaatt tctcttacta tagacttttt
     5761 atatgccttc atgtaaaact attcactcct accactctat ttcttcaact gagattggat
     5821 tagaattaat ctcaatactt ctaacttttg catcattaat cttaataacc ttgtcagcca
     5881 taggtgctat tgctgagtta tgagttatta ttattaccgt agttcctatc tttctacatg
     5941 tatcttgaag tatttttaaa atttgcttac ctgtactata atctaaagct cctgttggct
     6001 catcacaaag taaaagtttt ggattttttg caatggctct agctatagag accctttgtt
     6061 gttctccacc agataattgt gctgggaagt tatcttttct atgtccaagc ccaactaact
     6121 ctaaagtttt ctcaacatct aaggcattat ttgaaatttg agtggcaagt tccacatttt
     6181 cctttgcagt taagttttgt actaaattat aaaactgaaa tacaaatcca acatcatatc
     6241 ttctatactt agtaagctct ctattactaa atttagatat atcagtttca tcaatgatta
     6301 ttttaccttc atcacagcta tccattcccc ctaaaatatt aagagtagtt gattttcctg
     6361 ctccacttgg tcctaaaata acaacaaatt ctcccttatc aatagaaaaa ttaattccat
     6421 tattagcaac aactatgttt tccccaactt tatatctctt gtgaacatct attacatcta
     6481 tatatgccat cttattcctc cctaaactaa caattacctc ataaaaaatt tactatatac
     6541 tcaaagttat taaatagaca ctttgttcgt ttaattttat aataaatagt ataagttaag
     6601 aaaatatgct aaacaataat cacataattt cttaacgtta ttaaatgcac atttaaaatc
     6661 aatttgttga tttaataaca tttaacaata aattatctaa attatgtttt aaggtaaaat
     6721 ttattttcaa atttatctta aagacaagtt ttaatacata gtaaacactt tctttaaaaa
     6781 ttacgtaagt ctaaaaattt ctttaaaatc aataactttt taaaatatat aaactaaagg
     6841 aaatatattg gtattatata aatgagatgt taaaaggaga ggtatatatg gctggtgtta
     6901 gcggaaatcg tagaacttta tatactaaga aagttattaa ggaaagctta atagaacttc
     6961 ttaaaactag agaaattcat caagttactg ttactgatat ttgtaaaaaa gctgatatta
     7021 ataggggaac attttatact cactataaag atgcctttga cttatttcaa tctatggaag
     7081 acgagttatt ccatcaaatt ttaaaatata taaaagaaat tcccatagaa gaatataata
     7141 gtttactact tcttaaggta cttaaattaa ttaaggaaaa taaagatttg tgcaaagttc
     7201 tattttgcaa tcagagagat agcagtatat taaatcgtat tttaaatatt gcagatcaaa
     7261 tagatgtaat gaagctattt aataatcata atttaataaa taaaaccatt gctaattact
     7321 atatgagata ttctgttgga gggtgtattt caatcattga aacctggtta gaaaatgatc
     7381 ttcctgaatc tccagaggag cttgtccaaa taattaatgg tataaatcaa atgagtagct
     7441 aaaatatatt caaaataatt gagactatag taagctttcc ataaaacaaa actttctata
     7501 gtctcaatta atttctatat gttctttatt tatctgtaat atccatactc acaaagaagc
     7561 tcatggcaac tatttacttc ctcatcagtg aaaagttcat tgtatttact ttcaacaata
     7621 gtagcttcca tggtaagatc taaacgattt gccttttgta atttatcaaa accatctgaa
     7681 actctacctt tacgtaaaat ttcttgaact gttcttactc caccaaactt ctctattgtt
     7741 aataaaagtc ttttttgagg acaaccatac tctttctctg ctatttcaca tttcttaata
     7801 agctcttttt caaaattctt ttctaatgac ataatcttta ctccattaat ttaatttcat
     7861 ttcccttata atactatatt tatctatata attctaatat aacttcaaaa taatagaggg
     7921 aattatgagt tttttcataa ttccctctta gttttattat taactatttg ctttcttatt
     7981 tatttttaat tacttcttct actgcctcaa cttctattac ctcacaattt tcattacttt
     8041 cttttttaca atacttaatg aaaattccaa aaagaactaa ggcaaatacc acacctgcta
     8101 tattagaaaa tgttgcattt aatctaaatc cttcaggagc ttgtaatata tatgaacaag
     8161 taactgctga catgaaaact gctggcacta aggttatcca ataattatta tgagctttta
     8221 acatataagc agatgccgcc caaagcatta tcattgctaa agtttgattt gaccatgaga
     8281 aatatctcca tattatgcta aaatcaataa aagttaatgc tatacccaca acaaatagtg
     8341 gtatggctat cttaaatcta tcaataacct tatcttgttt aagtttaaaa gcatcagcaa
     8401 ttgtaagtct agcacttctg aaagctgtat ctcctgagga tataggacaa gccactaccc
     8461 ctaatattgc taatatggca cctacttttc ccattactgt attagaaata gtattaacta
     8521 cagtggctgg tcctccagct tttaaaattt cttgtaatcc atttgtatca ccaaagaatg
     8581 acatagctgc cgctgcccaa accaaggcta taactccctc tgcaatcatt gcaccataga
     8641 atacttttct tgcctctgat tcttttttta cgcatctagc cataataggt gattgtgtag
     8701 cgtggaatcc agatatagcc ccacaagcta ttgttataaa taagaatggg aatattggtg
     8761 ttccttctgg atgaaagttt gataattgaa tttctggaat attatatcct tcaattacaa
     8821 ttccagcacc tattcctata gccattatta aaaggcaaag tccaaatata ggataaattt
     8881 ttcctataac cttatctact ggtaaaacag tggctgctat ataatatacc actataataa
     8941 ctataaatat atctctgtct attcctgtta aagtttttaa aagatctgct gggcctgtta
     9001 agaaaactac cccaactaaa attaataata ctacagaaaa tataaccatt atttttcttg
     9061 gtatttctcc aaggtattta cctacaagtt cagcaactga caccccttta tttcttatag
     9121 ataaaactcc tgtgaaatag tcatgaacag ctcctgcgaa tatacatcca aaaactatcc
     9181 ataaaaatgc tacaggtccc cataacgctc cagatatagc tccaaatata ggaccaaggc
     9241 cagcaatatt aagaaactga attaaaaaaa ttcttggcca actcattggt acatagtcta
     9301 ctccatcttc catagactca gcaggtgttt caattgaatc acttgtacca aaggtcttgt
     9361 ccacaaattt tccataagta aagtagccca caattaataa tactaatgat aataaaaatg
     9421 atatcatttt tctcatcccc ttttttaatt gaattaattg ttacaattta attatactcc
     9481 tttagtaaac gtattcataa ttctttttca tcataaaatc gccttttcag actgaatacc
     9541 cctgaaaatt taggtaataa ctttaaattt tggctattta ctattttttt aaaactctct
     9601 atactattgg tgaaaaataa ccattttagt ttacaatata attctaataa taaaaaagtg
     9661 tatattaaaa gcttaaaaaa caaaacttat tgataatttt taataatcct caaaccacta
     9721 taagttttct tgtagccttt taatatacac attattttta taacttcaat taaaatttat
     9781 ttcctatagt atctataaac tcttttacaa tttcatttat tgtatcttct gcttgagggt
     9841 atattccaag gttatctaaa aacgcatggt ttaccccttc atatcttatt atttttgttt
     9901 ctactcctgc tttatgaata agcttaccat aagcttctcc ctgtactctt aaataatcgt
     9961 attcagaaac tgcaattaat gtttttttag cctttgaaag atttttatta aatatcggtg
    10021 ataaatcttc tcttctagga tctaactctc tttgtatgta aagtttttgt actaaatcct
    10081 cacaatttct taaagaatta ataaaattat atattttttc ttccttatat ggattctcat
    10141 ataaatctat atcccaatta aaaaatggat ttttctctac aaaagtaact acaggatata
    10201 caagcccttg aaactttata tttgaatttt cattagccac aattgttgat aaattagctc
    10261 ctgcactctc ccctactatt cctattttat ttttatctat tccataaaaa aaattatttt
    10321 cttcaatata ttcaacaact tttctacaat catttaatcc tagaggatat ggcttttctg
    10381 gtgccaagga ataatcaacg cttattacaa tcccattaat tccctcagct ataagtttac
    10441 aaggattttc agaaacatct aagcttcctc caataaatgc acctccatgt atataaacta
    10501 tgcatggtaa attttctttt aatccttctt tcttataaac tctacaatta actccattta
    10561 tattttcttt actcatcaat atattacttt tgcagagatt taaatttaat aatcccatat
    10621 tatctctcat atctccaaca gcttccccat taaacacata aggatttcct gatttttcag
    10681 gtcttttata ctcatcaaaa tatttaattc ttctatctat tcttgtccct tcaactggat
    10741 ctggtattgg cttaactaaa atattaacac catcttcatt aataaatctt gcattctcat
    10801 tcatggcttt tattaaatca ctttggctca tgtggtcaag ctcaaaaggt ttaagtctct
    10861 ctacaatata atataaagaa atattttcta ccatatattc ttctaattct ttatcataat
    10921 taatctcttt cttatcttta gaaacaccta gtctcttttc aacaattttg tctaaatcta
    10981 aatatccatc taaaaatgta ctctttctaa tccaagagtt tatctcattt cttatatact
    11041 ctaaattttt attttcacca ctaaatggag gtattgttgt aaatataatt ctcgccctat
    11101 ttttatgagc aatctctatt aatctcacaa atctttttat aattagattt aaattgtcct
    11161 catcttcttt taatttattg cactctaacc taattaagtc attaatacta tttacaacta
    11221 aaaccaaatt agaattattt ttactgaaaa cctcttcatc aaatctatca gataatactt
    11281 ttttaa
//
LOCUS       DAEVGI010000075        11259 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEVGI010000075 DAEVGI010000000
VERSION     DAEVGI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.075"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            29..691
                     /locus_tag="K8M77_001611"
     CDS             29..691
                     /locus_tag="K8M77_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HBI7086106.1"
                     /translation="MKPFIKKEDFNFIKKCITDLHGTLRNCTDPNIIETNKAYINEKI
                     LSRFSELSNEEKELIDINKITDPLHIDKYLEDLNEYVYGMTAIKSNAITKLFRKEKKL
                     KIPNLDFESSKKVYLGWIDEGSRKLFIAYNLNGKLTGMACKIPSYNSSNNHICTLCNH
                     IGNDTEVAFVSALCKTSNPEQGTYRSIGFDICLDSEKCNERITSTDKLEKLLKDVNNI
                     KK"
     gene            complement(850..2181)
                     /locus_tag="K8M77_001612"
     CDS             complement(850..2181)
                     /locus_tag="K8M77_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBI7086107.1"
                     /translation="MKEIKLNRIDIFSIVLGAIIGWGSFMLPGTKFLKESGVINTFLG
                     LFLGVICIIIIEKNYLIMMQTHDEEGGEFSFTYNNLGKKHGFIVGWFLTLAYFTMIPL
                     NATAFPLVIKKIFGGILEFGYLYNVAGYNIYLGEILTSSIIIIAFAALNLNGIKKTSK
                     VQNLIIFSLISMTFIVLVGMIIKGNRIDFINTYINKYSFDLSQIIKVFAITPFAFIGF
                     DAIPQLSKELNFSKKKASRVAVISLVMGALIYNILNVITALAYSPEQASSLEWAAGSA
                     VLSTLGKGAFFLLIIALTAAVWSGINGFMICSSKLLGSIANYKMLPSKMGKVNKNGVF
                     SNAIIFITIVSLIAPWFGRQAIIWIVDMSSLGASVAYFYVSFIVLKESKNTKDKILAG
                     IGVVISIIFMLLLILPISPAALSKESLIALIVWCIIGFIAYYKIQKDNIEA"
     gene            complement(2270..3556)
                     /locus_tag="K8M77_001613"
     CDS             complement(2270..3556)
                     /locus_tag="K8M77_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HBI7086108.1"
                     /translation="MKKYTEYIIGFILLAAIIIIGKINLGSSMLFFRLLAGLGLGYAL
                     TRSFFGFAGGVNRSYKTGSTKLMRMLMILFVGTAIVSVCFFIGQDPTEYDLWVNPINL
                     GLILGGLLFGFGMAFSSCCASGVLTDVVTGLPRAFITLIFFGMGVYLGFPVQNSASWV
                     KDTMISTGTFENGVFLPDLFKWDGLEGYLGAIIVTIILACLTIWVAKLYEEKRKANNT
                     YTGVDTEILQEKKHEEKGEFKLFSEATYEKLFVRPWTLATGALVIGGIFILLMGVTKA
                     GWGASTPYGFWFGRALVAVGVSPESVATFAGKPVEMFTAPFFSNGMNVQNFGIILGTL
                     VALLLAGSFKKTFKAGLQISFKEALLYIVGGFAMGFGTRLSNGCNVGALYTPIANLSL
                     SGWIFLVFLVAGGVLGNIVYKKIMGNKCCKVEVNNK"
     gene            complement(3662..5362)
                     /locus_tag="K8M77_001614"
     CDS             complement(3662..5362)
                     /locus_tag="K8M77_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7086109.1"
                     /translation="MSKKIVIVGGVAGGASTAARLRRLDENNQIIMFERGPHVSFSNC
                     CLPYYLSGKVENHEDLVLMTPEKFYSQYRIDARVYTEVVSINRKAKEVTVRNVVSGQE
                     YTESYDKLVLSPGAKAIVPPIKGIEDVNVFTVRNVVDIAKLDSFIKDNNSKNISVIGG
                     GFIGVEVAENLHEAGYNVTLIEAMDQIMKPFDYDMVQVLHKEMYDKGVNLIVGDKVSS
                     FESGKVVLESGKKINSDAVVMAIGVAPETDLAREAGLEIGQTGAIKVNQNYLTNDKDI
                     YAVGDAVEVYNSLTNSISRLPLAGPAQKQARAVADHIHGRPSRNSGYIGSSCVQIFDY
                     NGASTGLTEGQIKAMNLSLNYDIVRVIPQDKVGLMPGSEPLHFKLIFEVPTGRILGAQ
                     AIGKGNADKRVDIIATLIKMGGTVEDLKDLELCYAPPFGTAKDVVNHAALVASNLLEG
                     TFKQVYVHEVRDLVESGACIIDVREVSEYEAGHIKGAKNLPLSEIRDRLDEIPTDRPV
                     YLHCRSAQRSYNACLALQHLGFDNIYNVSGGFMGISFYEYYNDKVQDREPIVTEYNFN
                     "
     gene            5712..6608
                     /locus_tag="K8M77_001615"
     CDS             5712..6608
                     /locus_tag="K8M77_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI7086110.1"
                     /translation="MNLTYLRSFYTTVKCNSISKAAKQLHLTQPGVSMQIQKLENDIN
                     FKLLNRSNTGVSLTSAGEIIFEFAESMLSIEDNLQKKLDELKNINSKLIISCCKSLGE
                     HVMPCSIYTFKEIHYKTDISMEIDNTSDILKKLTNHETNIGIIQCKPSDCKNLELIPL
                     MTDKLVLVGGRNTSFKSVNVEELYKLPLITREDGSANKLLVESSLKKNKINIDNLNFV
                     LSLNSPQSIKSSVSFGQGYSFLPAISLNHELRSGELKKIDINDLKLPFQYYIALRKNY
                     KLDYYEQKFVDFLTSKKRCFCY"
     gene            complement(6824..7522)
                     /locus_tag="K8M77_001616"
     CDS             complement(6824..7522)
                     /locus_tag="K8M77_001616"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBI7086111.1"
                     /translation="MKKWSNGAKNKKFVASYSGGKDSTLALYKAMQEGTAIGIIVMME
                     EDGERSRAHSLFPSVLKAQAEAIGLPLFTVATNWEDYEKIFVKKLKEAKDLGAEVLVT
                     GDIDVPEEDCWHERVTSGIGLGLGMPLWRKNHKEVVEEFVNLGFVTKIVTVNLNKGMK
                     KEDLGRILTFAYIKELEERGIDPCGEAGEFHTTVIGGPLFKKELRVKHGEIISEGNYM
                     YLTLELDDFKLSDE"
     gene            complement(7650..7826)
                     /locus_tag="K8M77_001617"
     CDS             complement(7650..7826)
                     /locus_tag="K8M77_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086112.1"
                     /translation="MKKCLYCGKDLEKEPKENYIENKVGYFCSEDHFDKYILSLTPEE
                     YIEVQNSFCVCSDD"
     gene            complement(8172..8636)
                     /locus_tag="K8M77_001618"
     CDS             complement(8172..8636)
                     /locus_tag="K8M77_001618"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI7086113.1"
                     /translation="MRERVLEIRELKNCEIDDIMRIWLESTIEAHCFIEEEYWKKNYE
                     VVRDIYIPMAKTFVYCDEEKIKGFISIIDSNFIGALFVHTKSQGREIGKSLVEYVKNK
                     YENIELAVYKDNKKAVEFYKKEGFKIIKEQENEDSGHLEYLMSYSKNSSKNQ"
     gene            complement(8802..9956)
                     /locus_tag="K8M77_001619"
     CDS             complement(8802..9956)
                     /locus_tag="K8M77_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HBI7086114.1"
                     /translation="MKKIWKYGVLLALPLVLAGCGNKPSEGSNKNKEITSIKWIVDGA
                     LEAQKGQSKNIGTAGLLYGKTKDYIVVGGGANFPEEPVAKGGAKKLYPDIYVLKNENG
                     SLKQVDHTTLDYEIGYGSSITTEEGIYYVGGSPDKEEGNNVSLITTDEKGKVSLKQVG
                     DLLFTFSDGFAVKKDKDIYLGLGKQDGKASNKVYKFNVETGKTEELKEIPGEPTRNQA
                     VAQLLGDDIYVFSGGDKIAYTDGYKYSIKDNSWTQVADVKVGNEEISLLGANSVKLNE
                     DEMLVIGGFNKAVYDDAVKNLNTLKDESLAEFKKEYFGRDPQDFAWNTKVLIYNSKNN
                     EWKSIGDVPFNAPCGEALILDRDNIFSINGEIKPGIRTNKIYSGELYYVN"
     gene            complement(10251..>11259)
                     /gene="splB"
                     /locus_tag="K8M77_001620"
     CDS             complement(10251..>11259)
                     /gene="splB"
                     /locus_tag="K8M77_001620"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBI7086115.1"
                     /translation="RILFEKNSLNYEMGRNIYNYFKKYKDIEIIELKNNRIKQNIPGD
                     DIKEFYKEGKSTIVVGVKRVGKFQSCKPSAHWQLPLLSGCVGNCQYCYLNTNLGDKPY
                     VKINVNVEDILNQAQKYIDERKPNITIFEGSATSDPIPVEPYTNSLKRAIEFFANNDF
                     ARFRFVTKYTDVDSLLGLDHNGKTEVRFTINTDFVINNYERRTASLCERIKASVKIAK
                     ANYPLGFIIAPVFIYEGWKEDYENLLKDLKEKLPSDLKHKLTFEVISHRYTTRAKNII
                     MDIFPDNKLPMDDEKRTFKYGQFGYGKYVYKKEDIHEIKEFFMNIINKYFPMSEIKYI
                     I"
BASE COUNT         3839 a         1970 c         1110 g         4340 t
ORIGIN      
        1 tcaaacttct aaaaggaggt atattttaat gaaacctttt ataaaaaaag aagattttaa
       61 ttttataaaa aaatgcatca ctgatttaca tggtacccta agaaactgta ctgaccctaa
      121 tattatagaa acaaataaag catatataaa tgaaaaaata ttaagtagat tttcagaatt
      181 atctaatgaa gaaaaagaat taattgatat taataaaata actgatccac tacatattga
      241 taaatattta gaggatttaa atgaatatgt ctatggaatg actgctatta aaagcaatgc
      301 aattactaaa ttatttagaa aagaaaaaaa attaaaaatt cctaatttag actttgaaag
      361 ctcaaaaaaa gtttacttag gatggattga tgagggaagt agaaaattat ttatagcata
      421 taacttaaat ggtaagctta ctggtatggc atgtaaaata ccaagctata attcaagcaa
      481 taatcacatt tgtactcttt gtaaccatat tggaaatgat actgaagtag catttgtttc
      541 agcactctgc aaaacttcta atccagagca aggaacttat agatcaatag gttttgatat
      601 ttgtctagac agtgaaaaat gtaatgaaag aattacttca actgataagc ttgagaaact
      661 tttgaaagat gttaataata ttaagaaata attgaaatct tacaaagtgt aatattgata
      721 atatcaaaaa ataacctaag gcttataata cttaccttag gttattttca tgtttgtttc
      781 aagatgatat tttctatttt aaatattaac taaataactt tatatgtttt atttatcata
      841 aaataaatgt tatgcttcta tattatcctt ttgaatttta tagtatgcaa taaaacctat
      901 tatacaccat actattaaag ctattaaaga ctctttagat aaggctgctg gtgaaatagg
      961 taaaattaat aaaagcataa aaattatact tattaccact cctattcctg ctaaaatttt
     1021 atctttagtg tttttagatt cttttaaaac tataaaagaa acataaaaat atgcaactga
     1081 tgctcctaaa gaggacatat ctacaatcca aattattgct tgtctcccaa accaaggggc
     1141 tattaaactt actatagtta tgaaaattat agcattacta aagactccat ttttatttac
     1201 ctttcccatc ttagaaggaa gcattttata atttgctata gatcctaata atttagaact
     1261 acaaatcata aatccattta ttccactcca tacagcggca gttaaagcta ttataagtaa
     1321 aaagaaggct cctttgccta aagtactcaa tacagcactt cctgctgccc attctaaaga
     1381 acttgcttgt tctggtgaat acgctaaggc agttataaca tttaatatat tataaattaa
     1441 tgcacccata actaaggaaa tcactgctac ccttgatgcc tttttcttag aaaagtttaa
     1501 ttctttactt aattgtggaa tagcatcaaa gcctataaaa gcaaaaggtg ttatggcaaa
     1561 tacttttatt atttgactta aatcaaaaga atatttatta atatatgtat taataaaatc
     1621 tattctgttt cctttaatta tcatgccaac taaaacaata aaagtcattg atattaagct
     1681 aaaaattatt aagttctgaa ctttacttgt tttctttatc ccatttaaat ttaatgctgc
     1741 aaaagcaatt attattatac ttgatgttaa tatttctcct agataaatat tatatccagc
     1801 tacattatat aaataaccaa attctaaaat cccaccaaat atcttcttga ttacaagtgg
     1861 aaatgcagtt gcatttaatg gaatcattgt aaaataagcc aaagttaaaa accatccaac
     1921 aataaaccca tgttttttcc ctaaattatt ataagtaaaa gagaattctc ctccctcttc
     1981 atcgtgagtt tgcatcatta ttaaataatt tttttcaata ataattatac atattactcc
     2041 taaaaaaagc cctaagaaag tatttattac tccagactcc tttaaaaatt tagttccagg
     2101 tagcataaat gatccccatc caattatggc tcctaaaact atagaaaata tatctattct
     2161 atttagttta atttctttca tcattatctc cattcattta agaaaataaa gctataatta
     2221 gttttatttt atattaacca attatagctt tttaaactat tttttatctc tatttattat
     2281 taacttctac tttgcagcac ttatttccca taatcttttt ataaactata tttcctaaaa
     2341 ctcctcctgc tactaggaat acaaggaata tccaacctga taatgaaagg ttagctattg
     2401 gtgtataaag tgctcctacg ttacatccat ttgataatct tgttccaaat cccatggcaa
     2461 aaccaccaac tatgtataat aaagcttcct taaagcttat ttgtaaacca gctttgaaag
     2521 ttttcttgaa acttccagct aaaagtaatg caactaatgt tcctaatatt ataccaaagt
     2581 tttgtacatt cattccattt gagaaaaatg gagccgtaaa catttctact ggttttcctg
     2641 caaaagttgc tacactctct ggtgaaactc caacagctac aagtgctcta ccaaaccaga
     2701 atccataagg tgttgatgct ccccatcctg ctttagttac tcccattaat agtatgaata
     2761 taccacctat tactaaagct ccagtagcaa gagtccatgg tcttacaaat aacttttcat
     2821 atgtagcttc actaaataat ttaaattcac ctttttcttc atgtttcttt tcctgaagaa
     2881 tttctgtgtc aactccagta taagtattat ttgcttttct cttttcttca tatagctttg
     2941 ctacccaaat tgttaagcaa gctaatataa tagttactat tattgctcct aaatatcctt
     3001 ctaagccatc ccatttaaat aaatctggta agaatacacc attttcaaat gttcctgtag
     3061 atatcattgt atcttttacc caagatgctg aattttgaac tgggaatcct aaatatacac
     3121 ccattccaaa gaatattaat gttatgaaag cacgtggtaa tcctgtcact acgtcagtaa
     3181 gaacccctga tgcacaacaa ctagagaatg ccataccaaa tccgaataat aatcccccta
     3241 atataagtcc taagttaatt ggattaaccc ataagtcata ctctgtaggg tcttgtccta
     3301 taaagaaaca aacacttact attgctgttc ctacaaataa tatcattaac attctcatta
     3361 attttgtaga tcctgtctta taacttctgt ttactccacc agcaaatcca aagaatgatc
     3421 ttgtaagagc atatcctaat cctaagcctg caagtaatct aaaaaatagc attgaactac
     3481 caagattaat tttacctatt attataattg ctgctagtaa tataaatcca ataatatatt
     3541 ccgtatattt cttcaactta attcccccta ttgatttgtt aattaacaaa ctattttatt
     3601 tttaataata ttatttttta ctatggtgtt ttaaaattaa ttaaaacacc atgtatttac
     3661 tctaattaaa attatattct gtaactatag gctctctatc ttgaacttta tcattatagt
     3721 attcatagaa gcttattccc atgaatccac ctgatacatt ataaatgtta tcaaatccta
     3781 aatgttgtaa tgctaaacat gcattataac ttctttgagc acttctacaa tgtaaataaa
     3841 ctggtctatc agttggtatt tcatctaatc tatctcttat ttcacttaaa ggaagatttt
     3901 tggctccttt tatatgtcct gcttcatatt cacttacttc tctaacgtct attatacatg
     3961 ctccgctttc aactaaatcc cttacctcat gtacataaac ttgtttaaat gttccttcta
     4021 aaagatttga tgcaactaat gcagcatgat ttactacatc cttagctgtt ccgaaaggag
     4081 gggcataaca aagctctaaa tcttttaagt cttctacagt tccacccatt tttattaaag
     4141 tagctattat atcaactctc ttatctgcat ttcctttacc aattgcttgt gctcctaaaa
     4201 ttcttcctgt tggaacttca aatattaatt taaagtgtaa tggctcactt cctggcatta
     4261 atcctacctt atcttgaggt ataaccctta caatatcata gtttaagctt aaattcatag
     4321 cttttatttg accttctgtt aagcctgttg aagctccatt ataatcaaat atttgaacac
     4381 atgatgaacc tatatatcca ctatttcttg atggtcttcc atgtatatga tctgctactg
     4441 ctctcgcttg tttttgtgcg ggtcccgcta atggtaatct agatattgag tttgttaatg
     4501 aattataaac ttccacagca tctccaactg catagatatc cttatcatta gttaaataat
     4561 tttgattaac ttttattgct ccagtttgtc caatttctaa accagcttcc ctagctaaat
     4621 ctgtttctgg tgcaactcct atagccataa caactgcatc tgaatttatt ttctttcctg
     4681 attctaatac tacttttcca ctttcaaaag agcttacttt atctccaact attaaattta
     4741 cacccttatc atacatttct ttatgtaaaa cttgaaccat atcataatca aatggcttca
     4801 ttatttgatc cattgcttct attaaagtta cgttatatcc agcttcatgt aaattctctg
     4861 caacttcaac tcctataaat cctccaccta ttactgatat gttcttacta ttattatcct
     4921 ttataaaaga atctaatttt gctatatcca caacatttct aactgtaaat acattaacat
     4981 cttcaattcc ttttattggt ggtactatag cctttgcccc tggtgaaaga actagcttat
     5041 catagctttc agtatattct tgtccactta ccacatttct tactgttact tctttagctt
     5101 ttctattaat tgatactact tctgtataaa ctctcgcatc aattctatat tggctataaa
     5161 atttttcagg agtcataaga actaaatctt catgattttc aactttccca cttaaatagt
     5221 atggtagaca acagtttgaa aaggatacgt gaggacctct ttcaaacatt attatttgat
     5281 tattttcatc taatcttcta agtcttgctg ctgttgaagc tccacctgct actcctccaa
     5341 caataactat ctttttactc attatatttc cacctttctt agtgaatatc taaacaaatt
     5401 ctctttgtta aatttttatc taatatgtgt gtaatttaat tacctctact tgatagtatc
     5461 atataactag gtttatacca taataaaaat atgttatact taataacact ttgttatata
     5521 ccttgcttta aacaaagaat ttactttaaa atcgacttat ataatactat ttatttttta
     5581 atgttaatta ttttaacaaa ctaaactttt aaaattatct agaaaacttc attattttta
     5641 tataaaagta tgaaataata aaaagactaa ctttaatata aatagtaaaa caatatttag
     5701 gaggtgaact catgaattta acttatctta gatctttcta tacaacagtt aagtgtaata
     5761 gtatttctaa agctgcgaaa caattgcatt taactcaacc aggagttagt atgcaaattc
     5821 aaaaacttga aaatgatata aattttaaac ttttaaatag aagtaatact ggagtaagtt
     5881 taacaagtgc tggtgaaata atctttgaat ttgctgaatc aatgttatcc atagaagata
     5941 acttacaaaa aaaattagat gaattaaaaa atattaattc aaaacttatt atttcttgtt
     6001 gtaaaagttt aggtgagcat gtaatgcctt gcagtatata tacatttaaa gaaattcatt
     6061 ataagactga tatctctatg gaaatagata atacctcaga tatattaaaa aaattaacta
     6121 atcatgaaac aaatataggc attattcaat gtaaacctag tgattgtaaa aacttagaac
     6181 ttattccatt aatgactgat aagctagttt tagttggtgg tagaaatact tcatttaaat
     6241 cagtaaatgt tgaagaactt tataaacttc ctttaataac aagagaagat ggatcagcta
     6301 ataaattatt ggtagaatca agcttaaaga aaaataaaat taatatagat aatcttaatt
     6361 ttgttttatc ccttaattct cctcaatcaa taaaatcttc tgtatctttt ggacaaggat
     6421 attcattttt acctgcaatt tctttaaacc atgaattgag aagtggtgaa ttaaagaaaa
     6481 tcgatataaa tgatttaaaa cttcctttcc aatactatat tgcacttaga aaaaactata
     6541 aattagatta ttatgaacaa aaatttgtag attttctaac ctcaaaaaaa agatgctttt
     6601 gctattaaat aaaaaaatct tttcagataa ataaaaaaag ttacgccatt atttaaaaga
     6661 acttatagta ttattaggaa taattcttaa ttattaaaag atatttataa taaatttgtg
     6721 taaactatgt aaaaagatat atttaaaatc atatttttta ttatttaatt ataaaaagtc
     6781 aaggcaagtt taatttagct taccttgact tttatttgat tccttattca tctgataact
     6841 taaaatcatc taactctaaa gttaaataca tatagtttcc ctctgatata atttctccat
     6901 gtttaactct taattctttt ttaaaaagtg gaccacctat aactgttgta tgaaattcac
     6961 ctgcttcacc acaaggatca attcctcttt cttctaactc tttaatataa gcaaaagtaa
     7021 gaatccttcc aaggtcttct tttttcattc ctttatttag gttaacagta acaatttttg
     7081 ttacaaagcc taaatttaca aattcctcca ctacttcttt atgattcttt ctccaaagtg
     7141 gcatacctag tccaagtcca ataccactag taaccctttc atgccaacag tcctcttctg
     7201 gaacatcaat atcccctgta acaagcacct ctgcccctaa atcttttgct tcttttaatt
     7261 tcttaacaaa aattttttca tagtcctccc aatttgtagc tactgtaaac aaaggtaatc
     7321 caattgcctc tgcttgtgct ttcaatacag atggaaatag actatgtgct cttgatctct
     7381 ctccatcttc ctccatcatt acaattatcc ctatagctgt tccttcttgc atagctttat
     7441 ataaagctaa agtactatct ttacccccac tatatgatgc tacaaacttt ttattctttg
     7501 caccattact ccattttttc atactttact aatgctacta aaaaaatata ttatctatta
     7561 agtagcttct ccttccttat agaaaaatag ttttttaaat taaatttcct ctaaactcaa
     7621 aattccttta agtataaaac ctatctttat taatcatcac tacatacaca gaaagagttt
     7681 tgaacttcta tgtactcttc aggagttaaa gaaagtatgt atttatcaaa atgatcttca
     7741 ctacaaaaat atcctacttt attttctata taattttctt ttggttcttt ctctaaatct
     7801 tttccacaat ataaacattt tttcattttt ctctctcctt tttatatctc atgtatttca
     7861 taacctaagt ctatatttta cttaatttat gcataaatgc tttataaaaa tataataata
     7921 agatttattt ttatgcattc catttaatct ttcctatatg taaattattg cacaattaca
     7981 ctacatattt ctatattcat ataaaaagtt atcaattaac ccccttaatt aattattttt
     8041 ataaattttt aatttctaca aaatttaacc tctttctaag tttaacctct ttctaaatct
     8101 aatttcttca ttataaatac taacaaaaaa cacctaagcc catttacaat aaacttaagt
     8161 gttaataatt tttattggtt cttactacta tttttagaat atgacattaa atattctaaa
     8221 tgacctgaat cttcgttttc ttgctctttt attattttaa aaccctcttt cttataaaac
     8281 tcaactgcct ttttattgtc tttataaaca gctaattcta tattttcata tttattttta
     8341 acatattcaa ctaaactttt tcctatttca cgtccttgac ttttagtgtg aacaaataaa
     8401 gcacctataa agttactatc tattatactt ataaatccct ttattttttc ctcatcacag
     8461 taaacaaaag ttttagccat tggaatataa atatctctta caacttcata atttttcttc
     8521 caatattctt cttcaataaa acaatgagct tctattgtac tttctaacca tattctcata
     8581 atatcatcta tttcacaatt tttaagttct ctaatttcca aaactctttc cctcatacta
     8641 ttcctataac ctctttaaat tatctagtcc tttgtaaaaa tatctttttt atactttgta
     8701 tcttcatttt ataaaaaatt tattagctta ttcaatatct tatctaaaaa atattttagc
     8761 cattaattta tctattgctg aatctcctaa aaatcttttt attaatttac atagtataat
     8821 tcacctgaat aaattttatt tgttctaatt cctggtttga tttctccatt tattgagaag
     8881 atattatctc tatctaatat taatgcttct ccacatggag cattaaatgg tacatctcca
     8941 attgacttcc actcgttatt ttttgaatta taaattagaa cttttgtatt ccaagcaaaa
     9001 tcttgaggat ctcttccaaa atactctttc ttgaattcag ctagactttc atcttttagt
     9061 gtgtttaaat tttttacagc atcatcataa accgctttat taaatccacc tataacaagc
     9121 atttcatctt catttaattt aactgaatta gcacctaaaa gtgaaatttc ttcatttcca
     9181 accttaacat cagcaacttg agtccatgaa ttatctttaa tgctatattt ataaccgtct
     9241 gtgtaagcaa ttttatctcc accgctaaat acatatatat catcacctaa taattgagca
     9301 acagcttgat ttctagtagg ttctcctggt atttccttta attcttctgt ttttcctgtt
     9361 tcaacattaa atttataaac cttattacta gcttttccat cttgttttcc aagtcctaaa
     9421 taaatatctt tatctttttt aacggcaaag ccatcactaa atgtaaaaag taaatctcca
     9481 acttgtttta aactaacttt acctttttca tcagttgtaa taagagaaac attatttcct
     9541 tcctctttat caggacttcc acctacataa taaattcctt cctcagtagt tattgaactt
     9601 ccatacccta tttcataatc taatgttgta tgatccactt gctttaagct tccattttca
     9661 ttttttaata catatatatc aggataaagc tttttagctc ctccttttgc tactggctcc
     9721 tctggaaagt tagctccacc tccaacaact atataatctt ttgttttacc ataaagtaac
     9781 cctgctgttc ctatattttt gctttgacct ttttgagcct ctaacgctcc atcaacaatc
     9841 cacttaatcg aagtaatttc tttattttta ttactaccct ctgaaggctt atttccacat
     9901 cccgctaaaa ctaatggtag tgcaagtaat accccatact tccatatctt tttcataatc
     9961 ttttacccct ttcaaatcta aaatatttta taaaaagtat tttaaacaac aatattaatt
    10021 tgaaaatatt tttctaatat ttaacattgt aatccttttc caaagaaatt tcaatacaat
    10081 aaagatattt tatggtattt ttttctatat atatttctta actctaaata aaaagaaaat
    10141 tattttcata aataaaattt attgaaaata attttctaaa gatttaaagg tgctttttta
    10201 attcatctta acttttagat acttttaaaa actctcagtt tgttaaattt ttaaataata
    10261 tattttattt cactcattgg aaaatattta tttattatgt tcataaaaaa ctcttttatt
    10321 tcatgtatat cttctttttt ataaacatat tttccataac caaattgacc atatttaaaa
    10381 gttctctttt catcatccat aggcaattta ttatctggaa atatatccat tattatattt
    10441 ttagctcttg tagtatacct atgggaaata acctcaaagg ttaatttatg ttttaaatca
    10501 cttggtaatt tttcttttaa atcctttaat aagttctcat aatcctcttt ccacccctca
    10561 tatatgaata ctggagcaat tataaaccct aatggataat tagccttagc tattttcaca
    10621 ctggctttta ttctttcaca aagagatgct gttcttctct cataattatt tattacaaaa
    10681 tcagtattta tagtaaatct tacttccgtc ttgccattat gatctaaacc taataatgaa
    10741 tcaacatctg tatattttgt tacaaaccta aatcttgcaa aatcattatt tgcaaaaaat
    10801 tctattgctc tctttaagga atttgtatat ggctcaactg gtataggatc agatgtggct
    10861 gaaccctcaa atatagtaat attaggtttt ctttcatcaa tatatttttg agcctgattt
    10921 aatatatcct ctacattaac atttattttt acataaggtt tatcccctaa attagtattt
    10981 aaataacaat attgacaatt accaacacag ccacttagta atggtaattg ccaatgtgct
    11041 gatggtttac aagactgaaa ctttcctact ctcttaacac caactacaat tgtactcttt
    11101 ccttctttat aaaattcctt tatatcatct ccaggtatat tttgctttat tctattattt
    11161 ttaagctcaa tgatctctat atctttatat ttcttaaaat aattatagat atttcttccc
    11221 atttcataat ttaatgaatt tttctcaaat aatattctt
//
LOCUS       DAEVGI010000076        11218 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEVGI010000076 DAEVGI010000000
VERSION     DAEVGI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.076"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(175..321)
                     /locus_tag="K8M77_001621"
     CDS             complement(175..321)
                     /locus_tag="K8M77_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086116.1"
                     /translation="MKYIILTGILALIWMFYMALFTAAAKEDRYFEEEFAKSIKEEGN
                     DNLE"
     gene            460..1809
                     /locus_tag="K8M77_001622"
     CDS             460..1809
                     /locus_tag="K8M77_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI7086117.1"
                     /translation="MNKKYDSLFKELISSYYSEDNFDEKLNDIVKNNFNSKEDAIDVL
                     SSLCGVDIDKNSDNIAYDIRKAITTQKIKKNIVDKVSVCTKNCSKESKGKCQSLCPFD
                     AILTDPIDNSKYIDPNLCQNCGICVQVCESGHFLDRIELLPIIDLIKNNETVIAAVAP
                     SIAGQFGENVSLDMLREAFIKIGFSDMIEVAFAADILSIKEAVEFNHHVEKTGDILIT
                     SCCCPMWVAMLRKCYKDLVKDVSPSVSPMIAAGRVIKKLNKDAKVVFIGPCIAKKAEA
                     REKDLVGDIDYVLTFEELKGIFEALKIDPSSMKGVPSIEYTSRGGRLYARTGGVSEAI
                     NDVVKELYPDKAKIFKAVQANGVKECKELLNKVQSGELKANFIEGMGCFGGCVGGPKR
                     IVDPSIGKKHVDEVAYNSPIKVATHSHTMDEVLLRLGINSLKSFEDKEKISIFEREF"
     gene            1891..2544
                     /locus_tag="K8M77_001623"
     CDS             1891..2544
                     /locus_tag="K8M77_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBI7086118.1"
                     /translation="MLSNIKGVIFDLDGTLVDSMGVWAKIDSDYLTDLGLEVPKNLKE
                     EITHLGFKEVAKYFKKRFNIASSEEEIMKTWHDMAYVEYKNNIKLKPGAREFLEQLKE
                     SDIKIGLATSNSYPLLEVCLKSNDIFHLFDSITITGEVPRGKDFPDVYLLAAERLGLE
                     PKECAVFEDILPAVKGALSAGMKVFAVEEHTVSEEEKSQIKEIVHEYIDSFNDLLVN"
     gene            complement(2680..4113)
                     /locus_tag="K8M77_001624"
     CDS             complement(2680..4113)
                     /locus_tag="K8M77_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI7086119.1"
                     /translation="MSNIFEVMLKKNIGDFTYEEKRELNNWLIENLKLKELIYSGLEL
                     TKVINEDGEETAYYENKITEEDILSGNFNELCSKTSKYIDDAISSILFEGQFNCFYEK
                     IKSILELEDFDEIIVFHASFGWNIEMKQRPQHLAEALSKKKVLYIYRSDVKNDNIYSI
                     KKIKDNLYVLNLDMYSLNVAFFEAIKSINKPKFVHVYATCLNSVDYEKIKTYMDKGFK
                     VIYDFVDELSSEISGYNITSKMIEDHEKLLRDKENVLVVSTAKKLKDIANKFRGENEN
                     NILAPNAVNLEDFKNHGNEIGEKIQPIVNKRKPIIGYYGALAKWFDYKLIEELAKERE
                     DYEIVLIGIDYDGSYDQSNLKNYSNISYLGMIDYKYLINYSRYFDVCIVPFIKNDITD
                     STSPLKIFEYMALEKPIVTTDINECKNYESCLISKDYDEFIRNIDKALKLEVDNKEYF
                     NILRREAEENTWDNRAELIKEAIKNLE"
     gene            complement(4228..5409)
                     /locus_tag="K8M77_001625"
     CDS             complement(4228..5409)
                     /locus_tag="K8M77_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086120.1"
                     /translation="MEKAIEVKNLCKNYRVYDSKSSRIRNIINPFAKKEVKEFKALTD
                     VSFHVNKGEIVGIIGNNGAGKSTLLKILTGVSFPSSGEVNVNGRVSSLLELGTGFNPE
                     LTGEENIYFNGSLMGLTQDEIDKVKDNIIEFADIGEFINQPVKSYSSGMYARLAFAVA
                     ININPDILIVDEILSVGDVGFQKKCMDKFNEFKEAGKTVLYVSHGLETVQTFCERAIW
                     LEKGRVVDIGPSFDIVEKYYDKLMKSDDKEELETGKFVELINVEILDDKKVYKKGESI
                     EFLVEYEVFNPSVKNAGITIEMRKAYRKPCEYRNADQFIFSVNSNVDEFTIPWKKGRN
                     KITFKIDSLDVKEGIYYLDAIFSESQNLVALETQEDVVSFEVKNEGESEGFVFLDSTW
                     T"
     gene            complement(5423..6658)
                     /locus_tag="K8M77_001626"
     CDS             complement(5423..6658)
                     /locus_tag="K8M77_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI7086121.1"
                     /translation="MSLKRSVKNLLRPILKPMLLKYREKGHEDQKQVFFDEITGAIKN
                     GNYDAIVIFDVYFGFEVKMFQRPQHIALNLAKENVLYFYKASPYVDKDIKTYKKMEEN
                     LYLVNTDLYWLQECLIDIVSQSGVPAFGQIYSTSFVEYDSWLKKFTDRNFKIIYEYVD
                     DLSDDIAGFKISDEIKASHKRMLEDTEKVYVVTTADKLYQEAKELRGENKLALVTNGV
                     QYEHFANIDCKKIPDKMKNIVNSKKKIIGYFGALASWFDYDLIKELAEEFKDDYEIVL
                     IGIDYDQSLGKSGILKLDNVHYLGTVNYNELPTYSKFFNVSIIPFMVNEITEATSPVK
                     LFEYMALGKPIVTTALPECRKYESPLVSDSHEDFINNIKKAAELENSKEYIELLRKEG
                     NQNTWRQKAKDIKELILNS"
     gene            complement(6677..7960)
                     /locus_tag="K8M77_001627"
     CDS             complement(6677..7960)
                     /locus_tag="K8M77_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBI7086122.1"
                     /translation="MSVGRIARRTTEVLKEDGLIEVIKRVLRYIKRRIKDKLDNNALT
                     YRKNYEEANAIFHKILKKEKFKAVVVFDSRVGWNIPLFQRSQHMANELTDDGYLYFYR
                     TSEQFDPHVRTVEKLKDRLYLVNMANFALQNAMFDLLKEYKGHKFLSLYSTDVYLDEK
                     YIKEKYIDNGFKIIYEYIDELSDEISGHLPDFVYDRHKNIIEDKSNIAVGSADKLIEE
                     IEEIRGKENVAMITNGVQYDHWQYRSDEVPEKLKDIVSKGNPIIGYFGALAKWFDYEL
                     LKKVAKERPNYEIVLIGFLYDNSFKDSKIDELENVHYLGIVDYKELNQYSQYFTISTI
                     PFLLNDITESTSPVKLFEYMAMGHPIVTTDMRECRKYKSVLIGKSHEDFIEKLDFALT
                     LDKKDEYYNYLKEEALANTWREKAAILDRLIEKNI"
     gene            complement(7974..9146)
                     /locus_tag="K8M77_001628"
     CDS             complement(7974..9146)
                     /locus_tag="K8M77_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI7086123.1"
                     /translation="MSVAIRVKDLTKKYKLYQKRSERLANAFGKEKNIKEFYALKGVS
                     FEISKGECVGIIGHNGAGKSTLLKLLTGVAFPTSGEIEINGRLASMLELGSGFNPELT
                     GIENIYFNGSLNGLTKEEIDGKLKDILEFADIGNFIEQPVKNYSSGMFARLAFAVAIN
                     VDPDILIVDEILSVGDVGFQVKCMEKFNEFKKKGKTILYVSHGLGTVKKFCDRAIWLQ
                     KGEVIDDGNSVIVVERYYNLNFNPANIEQLKDHKSDIINSIVVKSNKKNVEYLEKLDL
                     EVEYDLISNDLKDPNIVLEFRKTDYEPGTTRGNDQFVCGINSKDNIKNIPFNLGVNKL
                     KVSLNKINLVAGVYYIDFVLRDGEEEVYRKINVYSFTLNDKYRGEGFLILGHSWGN"
     gene            complement(9164..9976)
                     /locus_tag="K8M77_001629"
     CDS             complement(9164..9976)
                     /locus_tag="K8M77_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086124.1"
                     /translation="MKDWLEIEKSIFSHWRMIFSISKKEFKKEFAGSKLGMAWAIIRP
                     LAMITVFWMIFSTGMRTTSSNVQVNYLVWLVSAYIPWIFISDVIMSGAGSIRVNSFLV
                     KKVKFPVEILPTIRIVISFYTFLILLVISFIIFAVNGNLNLINIPKLIYAIIITIIFL
                     AALSRLLSAWVVMSIDILHGISVFMQFLFWMCPVMWQAGELPVGSSMPWIEKILKLNP
                     LYYLAVLFRDAYLGTHTVSLAYGVYFWVVTIILFIFGSKVFNKFRPEFDDVL"
     gene            complement(10388..11134)
                     /locus_tag="K8M77_001630"
     CDS             complement(10388..11134)
                     /locus_tag="K8M77_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI7086125.1"
                     /translation="MEKKVLATVGNTEITSDYIDEIIARYPAQQQAMLASDEGKRQVL
                     EQAIAFELMSEFAKETGLDKTEEFKDQLNKFAKELLAQMVMKKTLSSVTVTDDEAKAF
                     YEEHKENFVELETVTAKHILVASEEEAKKVEEEIASGSITFEDAANKYSSCPSKEQGG
                     NLGSFSKGMMVPEFEEAAFNLELGVVSAPVKTQFGYHLIKVEDKTEAKTKAFEDVKEQ
                     VVNMLIQERQQKKYLELIKELREKYGVKLA"
BASE COUNT         3831 a         1887 c         1225 g         4275 t
ORIGIN      
        1 aaggaattgc aacagcaatt ccttttttta ttttatataa aattttaata ttaatactcc
       61 agttaaatct atttagactt aactggagta ttatttttat tagtagataa ttttaatata
      121 aaatatataa tctactttaa tcttattaat aaattatatt cttaaacata ttttttattc
      181 taaattatca tttccctctt ctttaatact ctttgcaaat tcttcttcaa agtacctatc
      241 ttcctttgcg gctgcagtaa ataaagccat ataaaacatc catataagtg ctaatatccc
      301 agttaatata atatatttca ttgtatatct ccctcttttt aaattagtat ctacttaaaa
      361 tactttccaa aacataaaaa tttatacact ccactaatac aatttgaatt tttgtacata
      421 ataattttat taaaatgatt gtaggaggat tgatctatta tgaataaaaa gtatgactct
      481 ttatttaaag aacttatatc ttcatattat tcagaagata actttgatga aaaattaaat
      541 gatatagtaa aaaataattt taatagtaaa gaagatgcta ttgatgttct ttcatcccta
      601 tgtggagttg atatagataa aaactcagat aatattgctt atgacataag aaaagctata
      661 acaacacaaa aaatcaaaaa aaatatagtt gataaagttt ctgtatgtac aaaaaactgt
      721 tcaaaagaaa gtaaaggtaa atgtcaaagc ctttgtccct ttgatgcaat actgactgat
      781 cctatagata atagtaaata tatagatcca aatctatgtc aaaattgtgg tatatgtgtt
      841 caggtatgcg aaagtggtca cttcttagat agaattgaac ttcttcctat tattgattta
      901 ataaaaaata atgagactgt aattgccgcg gtagctcctt ctatagctgg acaatttggt
      961 gaaaatgtct ccttagatat gctaagagaa gcatttataa aaataggctt ctcagatatg
     1021 attgaagttg catttgccgc tgatatactt tcaattaaag aagctgttga atttaatcat
     1081 catgtagaaa aaactggaga tatattaata acatcttgct gttgccctat gtgggtggcc
     1141 atgttaagaa agtgttataa ggatttagta aaggatgtct ccccttctgt ttcacctatg
     1201 atagctgctg ggagagtaat aaaaaaatta aataaagatg ctaaggtagt ttttataggt
     1261 ccctgtatag ctaagaaagc tgaagcaaga gaaaaagact tagttggtga catagattat
     1321 gtcttaactt ttgaagaatt aaagggcata tttgaagcat taaaaataga tccatcatct
     1381 atgaaaggtg ttccctctat agaatacacc tctagaggtg gaaggcttta cgctcgaact
     1441 ggaggggtat cagaggccat aaatgatgtg gttaaagaac tctatcctga taaagctaag
     1501 atatttaagg cagttcaagc taatggagtt aaagaatgta aagaactctt aaataaagtt
     1561 caatctggtg aattaaaagc aaactttatt gaaggtatgg gatgttttgg aggatgcgtt
     1621 ggtggtccca aaagaatagt agatcctagc ataggtaaaa aacacgttga tgaagttgct
     1681 tataattctc caataaaagt tgctactcat agccatacta tggatgaagt cctattaaga
     1741 ttaggaataa attcattaaa gtcttttgag gataaagaaa aaatatctat ttttgaaaga
     1801 gaattctaaa acatatttac ttataagctt aatattaacc ttaccttcta tataatttat
     1861 atgttaaact gtattggggg tgaggcaaca atgcttagca atataaaagg tgtaatcttt
     1921 gatttagatg gaactttagt tgattcaatg ggagtatggg ctaaaataga ttctgattac
     1981 ctaactgacc ttggtcttga agttcctaaa aaccttaaag aagaaatcac tcatttaggc
     2041 ttcaaagaag ttgctaaata ttttaagaaa agatttaata tagcaagttc agaagaagaa
     2101 ataatgaaaa cttggcatga tatggcctat gtggagtata aaaataatat caaacttaaa
     2161 cctggtgcaa gagaatttct tgagcaatta aaagaatctg acataaaaat aggattagct
     2221 actagcaact cttatccttt actagaagtt tgcttaaaat caaatgatat ctttcattta
     2281 ttcgattcaa taacaataac aggtgaagta cctagaggaa aggattttcc tgatgtttac
     2341 ttgttagctg ctgaaagatt aggcttagag cctaaagagt gtgcagtttt tgaagatata
     2401 ttaccagcag ttaaaggagc tcttagtgct ggaatgaaag tttttgcagt agaagaacat
     2461 actgtttctg aagaagaaaa atcacaaatc aaagaaattg tacatgaata catagattca
     2521 tttaatgatt tacttgtaaa ttaatattat acttacataa taaaactata aaaattaaac
     2581 tacataataa aaagataaat tacttatgta gttataataa taaaaactca atttaaaact
     2641 tagtaatgtt ttaaattgag tttttttgta aaatctattt tattctaggt ttttaatagc
     2701 ctcttttatt aattcagctc tattatccca ggtattttct tcagcttctc ttcttaagat
     2761 attaaaatat tctttgttat ctacttctag ctttaaggcc ttatcgatat ttcttataaa
     2821 ttcatcataa tcctttgaaa tcaaacaaga ttcataattt ttacattcat ttatatcagt
     2881 ggttactatg ggcttttcta aagccatata ctcaaatatt tttaatggtg atgtagaatc
     2941 agttatatca ttttttataa aaggtacaat acatacatca aaatatcttg aataatttat
     3001 taaatattta tagtcaatca taccaagata agatatattt gaataatttt ttaaattact
     3061 ttgatcataa gacccatcat aatctattcc tattaaaact atttcgtaat cttctctttc
     3121 ctttgctaat tcttcaatta atttataatc aaaccactta gctaaagctc cataataacc
     3181 tattataggc tttcttttat taactatagg ctgaattttt tcacctattt catttccatg
     3241 atttttaaag tcttctaaat taacagcatt aggagctaaa atattatttt cattttctcc
     3301 cctaaattta tttgcaatat cttttaattt ttttgcagtt gaaacaacta atacattttc
     3361 tttatctctc aagagttttt catgatcctc tatcatttta gaagttatgt tatatccact
     3421 tatttcacta gataactcgt ctacaaagtc atatatgacc ttaaaacctt tatccatata
     3481 tgtttttatc ttttcgtaat caactgagtt taaacatgta gcataaacat gtacaaattt
     3541 aggcttatta attgatttta ttgcctcaaa aaatgccaca tttaaactat acatatctaa
     3601 attaagaaca tataaattgt cctttatctt tttaattgaa taaatgttat cattttttac
     3661 atcgcttcta tatatatata atactttctt tttacttaat gcctctgcta aatgttgagg
     3721 tctttgcttc atttctatat tccatccaaa ggaagcatga aaaacaataa tttcatcaaa
     3781 gtcttctaat tccaatatgc ttttaatctt ttcataaaaa caattaaatt gtccctcaaa
     3841 taatattgag cttatagcat catctatata tttagatgtc ttagaacata attcattgaa
     3901 atttcctgat aaaatatctt cttcggttat tttattctca tagtatgctg tttcttcccc
     3961 atcctcatta ataactttag taagttctaa tccagaatat ataagttctt taagttttaa
     4021 attttctata agccagttat ttaattccct tttctcttca tatgtaaaat ctccaatatt
     4081 cttttttaac ataacttcaa aaatattact cataagaatt ctccttatca aaagacttta
     4141 atatttttca ttattaacat cttttcacat aattagtaca taaaagagga tataagccat
     4201 ataaactatg gtcatatcct ctatttttta tgtccatgta gaatctaaga atacaaaacc
     4261 ttcagactct ccttcatttt tcacctcaaa tgaaactacg tcttcttgtg tttctaaagc
     4321 aacaagattt tggctttctg aaaatatagc atctaaataa taaattcctt ccttaacatc
     4381 tagagaatca attttaaaag taattttatt tcttcccttt ttccaaggta tagtgaattc
     4441 atcaacatta ctatttactg agaatataaa ctgatctgca tttctatact cacaaggctt
     4501 tctgtatgcc tttctcattt caatagtaat accagcattt ttaactgatg gattaaacac
     4561 ctcatattca actaaaaatt ctatagattc acccttttta taaacttttt tatcatctaa
     4621 tatttccaca ttgataagct caacaaactt tcctgtttca agctcttctt tatcatcact
     4681 tttcataagc ttatcataat atttttcaac tatatcaaag gatggtccaa tatcaactac
     4741 tcttcctttt tcaagccata tagctctttc acagaaggtt tgaacagttt caaggccatg
     4801 tgatacataa agaactgttt taccagcctc tttaaattca ttaaacttat ccatacattt
     4861 tttttggaat ccaacatctc caacggataa tatctcatct acaataagta tatctggatt
     4921 aatatttata gcaactgcaa aggctaatct agcatacata ccacttgagt aactttttac
     4981 tggttgattt ataaattctc caatatcagc aaactctata atattatcct ttaccttatc
     5041 aatttcatct tgagttaatc ccattagact tccattaaaa tatatatttt cttctccagt
     5101 aagctctgga ttaaaaccag ttcctaattc taaaagagaa gatactcttc cattaacatt
     5161 aacttctcct gatgatggaa atgaaactcc tgttaatatt tttaacaaag tagatttacc
     5221 agcaccatta tttccaatga tacctactat ttcaccctta tttacatgaa aagaaacatc
     5281 agttaatgct ttaaactctt taacttcttt ttttgcaaaa ggatttatta tgtttcttat
     5341 tctacttgat tttgaatcat aaactctgta atttttacaa agatttttta cttctattgc
     5401 cttttccatg atttctcccc cattaagagt ttagtataag ctccttaatg tcctttgcct
     5461 tttgtctcca agtattttga ttaccttctt ttctaagtaa ttctatatat tccttagaat
     5521 tctctaactc agcagctttc ttaatgttat ttatgaaatc ttcatgtgaa tcacttacta
     5581 aaggtgattc atattttcta cactctggta aagctgttgt aactataggc ttacctaaag
     5641 ccatatattc aaataatttt actggtgaag tagcttctgt tatttcattt accataaaag
     5701 gaattatgga aacattaaag aacttagaat acgttggaag ttcattatag ttaacagttc
     5761 ctaaataatg aacattgtct aattttaata ttccgctctt tcctaaagat tgatcataat
     5821 ctattcctat aagaactatt tcataatcat ccttaaattc ttctgcaagc tctttaatta
     5881 aatcataatc aaaccaacta gctaaggctc caaaatatcc tattatcttt ttcttactat
     5941 taacaatatt tttcatctta tcaggaatct ttttgcaatc aatattagca aaatgctcat
     6001 attgaactcc attagttaca agagctaatt tattttctcc tcttagctct ttagcttctt
     6061 gataaagctt atctgcagta gttaccacat aaactttctc tgtatcttca agcattctct
     6121 tatgacttgc tttaatttca tctgatattt taaatccagc aatatcatct gataaatcat
     6181 caacatattc atatattatt ttaaagtttc tatctgtaaa tttctttaac caactgtcat
     6241 attcaacaaa tgaagttgaa tatatttgac caaaagcagg aactccactt tgagatacta
     6301 tatctattaa acactcttgt aaccaatata gatctgtgtt aactagataa agattttctt
     6361 ccatcttctt gtaagtttta atatccttat caacataagg tgatgcttta taaaaatata
     6421 aaacattttc cttagctagg tttaaagcta tatgttgagg tctttggaac attttaacct
     6481 caaatccaaa gtaaacatca aatataacta tagcatcata atttccattc tttattgcac
     6541 cagttatttc atcaaaaaat acttgttttt gatcttcatg tcctttttct ctatatttaa
     6601 gtaacattgg cttcaatata ggtcttaata aattttttac acttcttttt aaactcatac
     6661 tattaccctc tcactcttaa atgttttttt caattaatct atctagtata gctgcctttt
     6721 ctctccaagt atttgctaga gcttcttctt ttagatagtt ataatactca tcttttttat
     6781 caagagttaa tgcaaagtct agtttttcta taaaatcttc atggcttttt cctattaata
     6841 ctgacttata ctttctacat tctctcatat cagtagtaac tataggatgg cccattgcca
     6901 tatattcaaa aagtttaact ggacttgtag actcagttat atcatttaat aaaaatggta
     6961 tagttgatat tgtgaagtat tgagaatatt gatttaattc cttataatca acaataccta
     7021 aataatgaac attttcaagt tcatctattt tactatcttt aaagctgtta tcatataaaa
     7081 atcctattaa tacaatctca taattaggtc tttctttagc tactttttta agtaattcat
     7141 aatcaaacca ttttgctaaa gctccaaaat atcctattat aggattacct ttgcttacta
     7201 tatcttttaa tttttctgga acctcatcac ttctatattg ccaatgatca tattgaactc
     7261 catttgttat cattgcaaca ttttcttttc ctctaatctc ttctatttct tctataagct
     7321 tatctgctga ccctacagct atattactct tatcttctat aatattctta tgtctatcat
     7381 atacaaaatc aggtaagtgt cctgatattt catctgaaag ttcatctata tattcatata
     7441 ttattttaaa accattatca atgtatttct ctttaatata tttctcatct aaataaacat
     7501 ctgttgaata aagagataag aatttatgac ctttatactc cttaagaaga tcaaacatag
     7561 cattttgtaa agcaaaatta gccatgttaa ctaagtaaag tctatccttc agcttttcaa
     7621 cagtccttac atgcggatca aactgctctg aagttctata gaaataaaga tatccatcat
     7681 cagtaagctc atttgccata tgttgagaac gttggaataa aggaatgttc catccaactc
     7741 ttgaatcaaa aactactacc gctttgaatt tctctttttt taatatctta tgaaatatag
     7801 cattagcctc ttcataattt tttctataag ttaatgcatt attatctaat ttatctttaa
     7861 tacgtctttt tatatatctt aaaactctct ttataacttc tattaaacca tcttctttaa
     7921 gaacttccgt tgttctccta gcaattctac ctacgctcat actattcctc cgattaatta
     7981 ccccaagaat gccccagtat taagaaacct tctcctctat acttgtcatt caaagtaaaa
     8041 ctatatacat ttatttttct atatacttcc tcttcaccat ctcttaaaac aaagtcaata
     8101 taataaactc cagcaactaa gttaatctta tttaaagaca cctttaactt attaactcct
     8161 aaattaaaag gaatattttt tatattatct ttactattta taccacaaac aaactgatca
     8221 tttcctctag ttgtacctgg ttcatagtca gtttttctaa actctaaaac tatgttaggg
     8281 tcttttaagt catttgaaat aagatcatat tcaacctcta aatctaattt ctcaagatat
     8341 tctacatttt ttttattaga tttaactact atactattta ttatgtcact tttatgatct
     8401 tttaattgtt ctatattagc aggattaaaa tttaaattat aatatctctc tactacaatt
     8461 acagaattcc catcatctat aacttcacct ttttgaagcc aaattgctct gtcacagaac
     8521 tttttcactg ttccaagtcc atgagaaacg taaagtattg tttttccctt tttcttaaat
     8581 tcattgaatt tttccataca tttaacttgg aaaccaacat ctccaacaga taaaatttca
     8641 tcaactatta atatatcagg gtctacatta atagcaacgg caaatgctaa tctagcaaac
     8701 ataccacttg aataattttt tactggttgc tctataaaat tacctatatc agcaaactca
     8761 agtatatcct ttaattttcc atctatctct tccttagtta aaccatttaa actaccatta
     8821 aaataaatgt tttctatacc agtaagctca ggattaaatc ctgatcctaa ttctagcata
     8881 gatgctaatc ttccatttat ctcaatctct ccagaagtag ggaaagccac tcctgttagt
     8941 aattttaaaa gtgttgattt accggcccca ttatgaccga taataccaac acattctccc
     9001 ttgcttatct caaaagaaac gccctttaaa gcataaaatt cttttatatt cttttcttta
     9061 ccaaaagcat tcgctaatct ttcagatctc ttttgatata acttatattt tttagttaag
     9121 tctttaactc ttatagcaac agacatattc tgtacctctc tttttataaa acatcatcaa
     9181 attctggtct aaatttatta aatactttag atccaaatat gaataatatt atagttacta
     9241 cccagaaata aactccatat gctaaactaa ctgtatgtgt tcctaaatat gcgtccctaa
     9301 ataaaacagc taagtaatat aaagggttta attttagtat tttctcaatc caaggcatac
     9361 tagaacctac aggcaattct cctgcttgcc acataactgg acacatccag aataaaaatt
     9421 gcataaatac agatattcca tgtaatatat caattgacat tactacccat gcagatagca
     9481 atcttgataa agccgccaaa aatattatag ttatgataat ggcataaatc aatttaggta
     9541 tatttattag atttaagtta ccattgactg caaatattat aaaacttatt actagcaaaa
     9601 ttaaaaatgt gtaaaaacta attactattc ttatagtagg taatatttct acagggaatt
     9661 taaccttttt aaccaaaaag gaattaaccc ttatggaacc tgcacctgac ataattacat
     9721 cagaaataaa tatccatggt atgtatgctg aaactagcca aaccaaataa ttaacctgta
     9781 catttgaact agttgttctc attccagttg aaaatatcat ccagaaaaca gttatcatag
     9841 ctagtggtct tattattgcc caagccatac ctaacttaga accagcaaac tcttttttaa
     9901 attctttctt ggatatgcta aatatcattc tccaatgaga aaaaatactt ttctcaatct
     9961 caagccaatc cttcaagttg ttcacctctg cttatcatct atttttttgt aaaataacta
    10021 aacctaatta taccacaaat acttaataaa tatttataca tttctcttac aaagtagcaa
    10081 aatatacttc ttgtttaagc cttttcttaa aagctacaaa tatagctaat acttgtttaa
    10141 ataaactaac ttcatataaa attcaaatta ttaaaaacta ttaaatataa atactagata
    10201 tataaattaa tcttaattaa taataaaatt actagaacat tcttattatt ctaataatat
    10261 aaattataat ttattaaaaa tatctaactt aagtattgtc gtttatacat attactacac
    10321 taaaaacaat aaaaaaagtt agatatagaa taaaattcta cacctaactt tttaatagag
    10381 tcttaaatta agctaattta actccatatt tttctcttaa ttcttttatt aattctaagt
    10441 atttcttttg ttgtctttct tgaattaaca tattaacaac ttgctcttta acatcttcaa
    10501 aagccttagt tttagcttct gttttgtctt ctacttttat taagtgatat ccaaattgag
    10561 ttttaactgg agcactaact actcctaact ctaagttaaa tgcagcttct tcaaattctg
    10621 gaaccatcat tccttttgag aatgaaccta agttaccacc ttgttccttt gaaggacagc
    10681 ttgagtattt gttagcagca tcttcaaaag ttattgaacc tgaagctatt tcttcttcta
    10741 cttttttagc ttcttcttct gatgcaacta agatgtgctt agctgttaca gtttctaatt
    10801 caacgaagtt ttctttatgc tcttcgtaaa aagcttttgc ttcatcatct gttacagtaa
    10861 ctgatgataa agtctttttc attaccattt gagctaataa ttctttagca aatttattta
    10921 attgatcttt aaactcttca gtcttatcta atccagtttc ttttgcaaat tcagacatta
    10981 attcaaaagc aatagcttgc tctaaaactt gtcttttacc ttcatcacta gcaagcatag
    11041 cttgttgttg agctgggtat cttgctatta tctcatctat atagtctgaa gttatttctg
    11101 tatttccaac tgttgctaga actttctttt ccatatatac tatctccttt ttaattaaag
    11161 ttttattttc ctatacttca tatattaaca tacatttaat gaattaaaaa gaaattct
//
LOCUS       DAEVGI010000077        11141 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEVGI010000077 DAEVGI010000000
VERSION     DAEVGI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.077"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..683)
                     /gene="ric"
                     /locus_tag="K8M77_001631"
     CDS             complement(<1..683)
                     /gene="ric"
                     /locus_tag="K8M77_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HBI7086126.1"
                     /translation="MEKLIRKDYSLGEVVTVYPAVVKKFNDMELDYCCGGSKSLELAL
                     KEKGVDVDKFVEDLNKEFKEFKFENSQYVDWREKSSEELISHIVETHHGETFRLLKEI
                     DPLMVKVFRVHFSHDPELLMKVHSLFGKLKCELEEHLLKEENILFPLMIKYDKAKNEK
                     EKKEIEEDIRIIVNEHEAAGDILKELAEVTDDYKVPEWGCISFKLLYDYLHDLEKDLF
                     IHIHKENNI"
     gene            complement(862..1674)
                     /locus_tag="K8M77_001632"
     CDS             complement(862..1674)
                     /locus_tag="K8M77_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HBI7086127.1"
                     /translation="MENKKVVLKDGQAKVIDSERINMLTDFLRRINKEGITKELREEG
                     LDIVKSIDPLELSIAEQNLIDDGMEPSELRHLCDIHMEILKDELEKLKSNISRGHVLD
                     TLVEEHTKILGLLEELEVVTSKIAKMDKYNKENAEFSKALEIVQGILDAELHHKREED
                     VLFPELEKREITGPTRIMRLEHNQLREKKRRLKELIKVVEYLDFEDFKDNLQSLSKEI
                     CFELKDHIFKENYILYPTALEAIKDEKLWENMREKCDDIGYCPFTPKECIKK"
     gene            2228..3607
                     /locus_tag="K8M77_001633"
     CDS             2228..3607
                     /locus_tag="K8M77_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBI7086128.1"
                     /translation="MTNILGLILSLILIMYLAYKGYSTIITAPIVALVTILITTGLDA
                     HLMASYTEIYMSGFASFIKNYFPLFMTGAIFAKLMEEVGYAKSIAHFITKKLGKEKSI
                     LAVVLSGALLTYGGVSLFVVAFILYPIATTLFREADIPKRLIPGTIALGAFTFTMTAM
                     PGSPEIQNVIPMKYFGTDTFAAPLIGLLASIMMITLGMIWLTRRAKSAKAKGEGYGNH
                     NDSMVNENYENLPSIFKAIIPIIIIFIVNLFFSKVYYENIDGSYLKEFNTTLSNVSGT
                     WSVIIAIVLADLFLIITNFKKIKNLKSVLDKGVTNSFKPLLNSSAIVGYGSVIKSLAV
                     FTLIQSVIFGVSSNPIISEALSVNLICGLTASASGGLGISLDALAPTYLQMSQAMNIS
                     PEILHRIASLSSGGLDTLPHNGAVITTLAICSLTHKEAYKDMFVTSVLIPIFTTAVIV
                     LLVSMKFAV"
     gene            complement(3750..6779)
                     /locus_tag="K8M77_001634"
     CDS             complement(3750..6779)
                     /locus_tag="K8M77_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBI7086129.1"
                     /translation="MILKNEYHEDISKLHVNMMPRRSYYVPFVDTDEALNIKDRSKQV
                     NFFSLNGKWEFNYFDSLQKVKEFDNINQINFSDNIDVPSLWQLKGYDYNQYTNVKYPI
                     PFDPPFVPTNNPCGIYKRDFEIEILPENYDYNINFEGVDSCFYFWINDNFVGYSQISH
                     SISEFDITEFLVKGKNTITVLVLKWCDGTYFEDQDKFRMSGIFRDVYILRRAKERIVD
                     YKITQSIDFSAKEGKLDLEILSNIGNPKGKYYLLNPNNHMIASGNIDNNKVQINIKNV
                     ELWSAEIPNLYTLLIETEHEVIKERIGMREIKIENSILKINNKKVKLRGVNHHDSNPT
                     KGYVMTYDDMILDLKIMKECNFNSIRTAHYPKSPIFYELCDEYGFYVMSEADIEIHGV
                     VELYGLGYLDNYNMIADDKVYEKVIIDRVDSSIVPFKNKSCIFMWSLGNESGFGCNFE
                     RGLEYARALDPTRPLHYEGAYYASKERENDFTNIDVISRMYISIEEIKDYFEKGIDKP
                     LILCEYAHAMGNGPGGLQDYDEMIQKYDQFAGAYVWEWCDHAILINEDVNGKKAYGYG
                     GDFEEENHDGNFCVDGLVYPDRTPHTGLLEYKNINRPIRAIEFDEVKKRVKFKNMLDF
                     RDVSEFLDVTYKVFLDGETIFGGNIDLESLKAKEEKWYDLSISELPKGIITILFQYKV
                     KNNNHLYEKGEVLGFDNFIIKNGVDNISSVDKIIKSTINEQKFYVEETVNKIKVKNNE
                     FIYNYNKNTGSFDFIQALGETFIDDPMKFIIWRAPTDNDRKIKNLWIEAGFNQITTRV
                     YNSKIKEFSNRVEITSDLSLIPPYRERVLDLKLTWSIYSEGLIKCHVKGNKNMKTPYL
                     PRFGVELKLNKSYEEVSYFGFGPYENYVDKNSSCYLGRFNSKVSEMHEDYIRPQENGS
                     HHYCREVAINNEKGKVYVLSENDFAFNVSHFSLNQLTNANHNFDLNEEEATYLIVDYK
                     QSGIGSNSCGPDLDEEYRLNEKEFSYDFYLKFVKENI"
     gene            7143..7989
                     /locus_tag="K8M77_001635"
                     /pseudo
     CDS             7143..7989
                     /locus_tag="K8M77_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459576.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(8079..9443)
                     /locus_tag="K8M77_001636"
     CDS             complement(8079..9443)
                     /locus_tag="K8M77_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7086130.1"
                     /translation="MDTLINKKVEKKEDVSIKTLITYSMGSLGNNMIYALISSYLLIF
                     LTDSFKVGAAAIGTLFLVARIIDAITDPIMGVIVDNTKTKIGKSRPYLFIVPIFMGIA
                     TIMCFSAPDLSYSGKIIWIYIAYIFWGISFTAMDIPYWSLSANITRSSSGKTKIVTSA
                     RTVAYVGNFIILTSTIPLVAIIGNWQTVAIIYVCFATIFTWVTAFGIREIKDNVEKKK
                     EKQGFKQFINLLKTNQPLRIVLLSMLVLELSGAIKNTISIYYVKYNFNAEMMIPVVSS
                     IGMAASILGGVMSPFLTKKLGKRNTALLGLLAGAAGSFLVFLLSYSSLPLMIVINFIS
                     GIFDGAGYITLTSMVADCVEYGEWKTGKRSEGMIFSLNIFKSKIASAIGGSLCGYILA
                     YIGYTANESQSTFTLNGIHLIQTLVPCAIVIISFILLRRYNLSEAEYEAIVDDLKNGV
                     TRIG"
     gene            complement(9783..9938)
                     /locus_tag="K8M77_001637"
     CDS             complement(9783..9938)
                     /locus_tag="K8M77_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI7086131.1"
                     /translation="MTDKIMTCRDCGSEFVFSVGEQEFYKEKGFDNEPTRCMSCRRAK
                     KQQQNRR"
     gene            10495..>11141
                     /locus_tag="K8M77_001638"
     CDS             10495..>11141
                     /locus_tag="K8M77_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086132.1"
                     /translation="MKKLKRAYKMEINPTDEQKSKIHQTIGVSRFVYNFYIARNKEIY
                     EREGKFVSGMDFSKWLNNEYIPNNQEMKWIKEVSSKATKQAIMNGDKAFIDFFKKAKG
                     FPKFKKKKNQDVKAYFPKNNKTDWTLERHRVKIPTLGWVRLKEFGYIPVNLIIKSGTV
                     SQKADRYYVSILVEERDIKISNSNTGMKISNTNNEGLGIDLGIKEFAVCSDGIKF"
BASE COUNT         3933 a         1713 c         1248 g         4247 t
ORIGIN      
        1 aatatattat tttctttatg aatatgtatg aataaatctt tttctaagtc gtgtaaataa
       61 tcatatagta gtttaaaact tatgcatccc cactctggaa ctttataatc atcagttact
      121 tctgctaatt cttttaatat gtcaccagca gcttcatgtt cattaactat gattcttata
      181 tcttcttcta tttctttctt ttctttttca ttctttgctt tatcatactt aatcattagt
      241 gggaataaaa tattttcttc tttaagtaaa tgttcttcta attcacattt tagctttcca
      301 aaaaggctat gaactttcat taaaagttct ggatcatgtg aaaaatgcac tctaaatacc
      361 ttaaccatca atggatctat ttcttttaat aatctaaaag tctctccatg atgagtttca
      421 actatatgac ttattaactc ttctgaagat ttttctctcc aatctacata ttgagaattt
      481 tcaaatttaa actctttgaa ttctttattt aaatcttcaa caaatttatc aacatcaaca
      541 cctttttctt taagtgctaa ttctaagctc ttacttccac cacaacaata atctaattcc
      601 atatcattaa atttcttaac aactgctggg taaacagtaa caacttctcc taaactatag
      661 tcttttctaa ttaatttttc cataataatt tcctcctaaa gttttaatca cacatttata
      721 acaaactatt tttgctagtg ttgattttta catagaactt taagttaatc ttaataataa
      781 agtaataaaa atactttaat aaactaaatt tcatctaaag aaccagctaa aatcaacatt
      841 caaccgaaat ataatcttaa actatttttt tatacattct tttggagtaa atggacaata
      901 tcctatgtca tcacatttct ctctcatatt ctcccaaagt ttttcatctt ttatagcctc
      961 taaagctgtt gggtataaaa tataattttc tttaaatata tgatccttaa gttcaaaaca
     1021 aatttcttta gataaagatt gaagattatc tttaaagtct tcaaaatcaa gatattcaac
     1081 tacttttatt aattccttta atcttctctt tttctctctt aattgattat gttctaacct
     1141 cattattctt gttggaccag taatctctct cttttctaac tctggaaata atacatcttc
     1201 ttctctctta tgatgaagtt ctgcatctaa aataccttgt actatttcta aagcctttga
     1261 aaattctgca ttttctttat tatatttatc catttttgct attttactag taactacttc
     1321 aagttcttct aataatccta atatttttgt atgttcttca actaaagtat ctaagacatg
     1381 tccccttgat atattgctct ttaatttttc aagttcatcc ttaagtattt ccatatgtat
     1441 atcgcataaa tgtcttaact cactaggttc cattccatca tctattaagt tttgttctgc
     1501 aattgataat tctaagggat caattgactt tactatatct aatccttctt ctcttaattc
     1561 tttagttata ccttctttat ttattcttct taaaaagtca gttaacatgt ttattctttc
     1621 agaatcaata acctttgctt gtccatcttt taatactact tttttatttt ccatatataa
     1681 atttcaatag cataataaat ttatactaac tacttcctcc tataaataaa aatttcttat
     1741 tatttattgg gctttttgcc tctctcttat gatgctatta taatttcaaa gtgcttatct
     1801 taaatatgac tggggtcaca ttgaaaaaat taaataaaaa actatgtaaa tatataatat
     1861 ttccaattcc atatattttt taatgagata ttcatcagta aacgattaac aaagaagcta
     1921 tttgctatta ttcacctctt aattttattt aatataaatt attatatgat ataatatttt
     1981 attaaatcaa aattattaat ttaattttat ggatttataa atataaaaaa ggaaaaataa
     2041 taaatgaatt taactaaaga aaggagatag tgctgtatca aaatataaaa ataaaactca
     2101 aaaatagtat gaagagaata tctagattaa ctttagaatt taaatcttta gcaaattcta
     2161 gtaattatct taatatgttt attagtttta taataattta ttttttagat gcagctattt
     2221 aatcataatg acaaatatct taggtttaat attatcctta atacttataa tgtatcttgc
     2281 ttataaaggg tactcaacaa taataacagc tcccattgta gcattagtaa ctatacttat
     2341 tactactggg ttagatgcac atttaatggc tagttacact gaaatatata tgtctggctt
     2401 tgcaagtttt ataaaaaatt attttcctct ttttatgaca ggagcaattt ttgcaaagtt
     2461 aatggaagag gtaggttatg ctaaatctat agctcacttt ataactaaaa aacttggaaa
     2521 agaaaaatca atattagccg ttgttctttc tggagcattg cttacttacg gtggtgtttc
     2581 cttgtttgta gtagcattta ttctttatcc aattgccact acattattta gagaagctga
     2641 tattcctaaa agacttatac ctggaacaat agctcttgga gcatttactt ttacaatgac
     2701 tgctatgcca ggttcaccag aaattcaaaa tgttatacca atgaaatatt ttggtacaga
     2761 tacttttgca gcaccactta taggattatt agcaagcatt atgatgataa ctcttggtat
     2821 gatatggctt acacgccgtg caaaatcagc taaagctaag ggggagggat atggaaatca
     2881 taatgattca atggttaatg aaaactatga aaacttacct agtatattta aagcaataat
     2941 tcctataata ataattttta tagttaactt attcttttca aaagtttatt atgaaaatat
     3001 agatggaagt taccttaaag aatttaatac aactttaagt aatgtttctg gaacttggag
     3061 tgtaataatt gctatagttt tagcagactt atttttaata ataactaact ttaaaaaaat
     3121 aaaaaactta aaatcagtat tagataaagg agttactaac tcatttaaac cactattaaa
     3181 ttctagtgcc atagttggat atggaagtgt aataaaatca ctagctgtgt tcactcttat
     3241 acaatcagtt atatttggcg tttcatcaaa cccaataatc tctgaagctt tatcggttaa
     3301 cttaatatgt ggacttacag cctcagcctc tggaggactt ggaatatctt tagatgctct
     3361 tgctccaaca tacttacaaa tgagtcaggc tatgaatatt tcaccagaaa tacttcatag
     3421 aatagcttct ctatcttctg gagggttaga tactcttcct cataatggag cagtcattac
     3481 tactttagca atttgtagtt taactcataa agaagcatac aaggatatgt ttgtaacctc
     3541 tgtccttatt cctatattta caacagctgt aatagtctta ttagtttcta tgaaatttgc
     3601 tgtttaatac taaaatttta aatattattt ttatattttg atataactct tttacagttt
     3661 ttattctata aaaaatgctg ttttatatag tgttttcaag cactatataa gacagcaatt
     3721 attttaaaaa attattatat ttaattattc tatatatttt cttttacaaa tttcaagtaa
     3781 aaatcataag aaaattcttt ttcatttagt ctatattctt catctaaatc tgggccacaa
     3841 ctatttgatc ctataccact ttgtttataa tctacaatta aataagttgc ctcttcttca
     3901 ttcaaatcaa aattatgatt tgcattagtt aattgattta aagaaaagtg tgaaacatta
     3961 aaggcaaagt cattttctga taaaacataa acctttcctt tttcattatt aatagctact
     4021 tctctacaat aatgatgact tccattttct tgaggtctta tataatcttc atgcatttca
     4081 gaaaccttag aattaaatct tcctaaataa caagatgaat ttttgtccac gtaattttca
     4141 tatggtccaa atccaaagta acttacctct tcataggatt tattaagctt aagctctaca
     4201 ccaaaccttg gtaaataagg tgttttcata tttttattcc ctttaacatg gcatttaatt
     4261 aatccctctg agtatatact ccatgttaac ttaagatcaa ggactctttc tctatatggt
     4321 ggtatcaagc ttaaatcgct agttatctcc accctatttg aaaactcctt aattttacta
     4381 ttatacactc ttgtagtaat ttgattaaag ccagcttcaa tccacagatt tttaatcttt
     4441 ctatcattat ctgtaggtgc tctccaaata ataaatttca ttggatcatc tataaatgtt
     4501 tctcctaatg cctgaataaa atcaaaggaa cctgtatttt tattataatt ataaataaac
     4561 tcattatttt taactttaat cttattaact gtttcttcta cataaaattt ttgttcatta
     4621 atagtacttt ttatgatttt atcaacagat gagatattat ctactccatt ttttatgata
     4681 aaattgtcaa agcctagaac ttcacctttt tcataaaggt gattattatt tttcacttta
     4741 tactggaata atatagttat aattcccttt ggtaattctg atatgcttaa atcataccat
     4801 ttttcttctt ttgcttttaa gctttctaaa tctatattac ctccgaaaat tgtttctcca
     4861 tctaaaaata ctttataggt aacatctaag aactcagaaa catccctaaa atcaagcata
     4921 tttttaaact taactcgttt cttaacttca tcaaattcta tggctcttat aggtctattg
     4981 atatttttat attctaataa tccagtgtga ggagttctat ctgggtaaac taaaccatca
     5041 acacagaagt ttccatcatg attttcctct tcaaaatcgc caccatatcc atatgctttt
     5101 ttaccattaa catcttcatt aataagtatt gcatggtcac accactccca aacatatgcc
     5161 cctgcaaact ggtcatactt ttgtatcatt tcatcatagt cttgtaatcc acctggtcca
     5221 tttcccatag catgtgcata ctcacataat attaatggct tgtctattcc tttttcaaag
     5281 taatccttga tttcctctat acttatatac atacgactaa taacatcaat atttgtaaag
     5341 tcattttctc tttctttgct agcataataa gcaccttcat aatgaagtgg acgtgtagga
     5401 tctaatgccc tagcatattc taatcctctt tcaaagttac atccgaatcc tgactcattt
     5461 ccaagagacc acatgaaaat acaagattta tttttaaaag gaactataga tgaatcaact
     5521 ctatcaataa taaccttttc ataaacttta tcatcagcta tcatattata attatctaaa
     5581 tatcctagtc catatagctc aacaacccca tgaatttcaa tatcagcctc actcataaca
     5641 taaaaaccat attcatcaca taactcataa aaaatcggag atttaggata atgagctgtt
     5701 cttatagagt taaaattaca ctctttcatt atttttaaat ctagaatcat atcatcatat
     5761 gtcataacat atcccttagt tggattacta tcatgatgat taactcctct taactttact
     5821 tttttattat taatttttaa aatagaattc tctattttaa tttctctcat acctattctt
     5881 tcttttataa cttcatgctc tgtttcaatt aataaggtat ataaattagg tatttcagca
     5941 ctccaaagtt ctacattttt aatattgatt tggactttat tattatctat atttccagat
     6001 gctatcatgt gattattagg atttaataaa taatatttcc cttttggatt tcctatatta
     6061 gataaaattt caagatctaa ctttccttct ttagctgaaa aatctatact ttgagttatt
     6121 ttatagtcaa ctattctttc ctttgctctt cttagtatat atacatctct aaaaattcca
     6181 gacattctaa acttgtcttg atcttcaaag taagttccat cacaccattt taaaactaag
     6241 actgtaattg tatttttacc ctttactaaa aattctgtaa tatcaaattc agatatgcta
     6301 tgggatattt ggctataccc aacaaagtta tcatttatcc aaaaataaaa gcaggaatca
     6361 acgccttcaa agtttatatt gtaatcataa ttttcaggaa gtatttcaat ttcaaaatct
     6421 ctcttataaa ttccacacgg attgtttgta ggaacaaaag gtggatcaaa aggaattgga
     6481 tattttacat tagtgtattg attataatca tatcccttta attgccaaag agatggcaca
     6541 tcaatattat ctgaaaaatt aatttgattt atattatcaa actctttaac tttttgtaag
     6601 ctatcaaagt aattaaattc ccatttacca ttaagtgaaa agaagttaac ttgcttactt
     6661 ctatctttaa tatttaaagc ttcatcagta tccacaaagg gaacataata acttcttctt
     6721 ggcatcatat ttacatgcag tttagaaata tcttcatggt attcattttt taatatcatt
     6781 ttaccctccc aatacattta aaataattat gtattcatga aacatgattg tatatttaag
     6841 aaacataatt ccatataaat catttttcaa aatagttttt acccataatt aaatgttaat
     6901 atgtaaatta atcttttaga atagttaaaa agttctaaaa tatgttataa tgtttcttat
     6961 aatcttataa attttaataa ctaatatata aagatatttc tttaaaatat tcttatattt
     7021 agaagaattt actttaaaat aaaaagcttt tatgttgata aactgctttg caaagctctc
     7081 atgtaaatgt ttaatataag actactataa aattggctaa ttttataggt taggaggtag
     7141 aaatgcaaat attgtggaaa aagtatgtta aagaaaactt tgaaatgaat gtagatgaat
     7201 gtggtataga acaaggtata ccaggattag gctacaacta tgaagtgtta aaagatattg
     7261 tcattcatta cgtaactaag ggttatggga cttttaaatt taatggtaag gtatataact
     7321 taaaaaaagg tgatatattt atactattaa aaggtatgca agttgagtat gtagcttcta
     7381 ttaatgatcc ttgggaatac tcctggatag gatttagtgg ctcaaatgct aatgaatatt
     7441 taaatagaac ttctattact aactcctgtg ttgctaattg taaagaaaac tcaaaaattc
     7501 cacagataat attaaatatg tgcgaaatat caaaaactta taatccttca aaatctgatg
     7561 acatactatt actaaaagaa ctttactcat tattgtacac acttatagaa gaattcccaa
     7621 aaccttttga atacaaagat aaggaattac acacatatat tcaagatgcc cttaatttca
     7681 ttaattctaa ttatatgcat agcataactg ttcaagaaat tgctgattat gtaaacttaa
     7741 gcagaagtta tttatataaa atgttcataa aaaaccttgg aatttctcct caaagatatt
     7801 taataaacct tagaatgtac aaagccactc ttttactaaa aggcactaaa cttcctatag
     7861 gtgaagttgc aagtagtgta ggctatagtg attcactatt attttcaaaa actttttcaa
     7921 aacatttttc aatgtctcca ctaaattaca gaaataatca aagtaaataa aacaagcata
     7981 tcaatttaaa atacagcttt aaaacaaaaa aaattccaaa aataaaaagt ataacagagg
     8041 ttttaattta cgtctctgtt atacttttta tattaatgtt aacctattct tgtaacgcca
     8101 tttttcaaat catcaactat agcttcatat tctgcctcag ataaattata tcttcttagt
     8161 aatataaagc ttattattac aattgcacat ggaactaaag tttgtattaa atgaattccg
     8221 tttaatgtaa aagtagattg actttcattc gctgtataac caatatacgc taagatatat
     8281 ccacataggg atcctccaat agcactagca atcttacttt taaatatatt taaagagaat
     8341 atcattcctt ctgatctctt tccagttttc cattcaccat actcaacaca atctgcaacc
     8401 attgatgtta aggtaatata gcctgctcca tcaaaaattc cacttatgaa gtttataact
     8461 atcattaaag gtaatgaaga ataggataat aaaaatacta aaaatgatcc agcagcacca
     8521 gctaataatc ctaaaagagc agtatttctt tttcctaatt ttttagttaa aaacggtgac
     8581 attactccac ctagtatgct agctgccata ccaattgaac taactacggg gatcatcatt
     8641 tcagcattaa aattatactt tacataatat atagatattg tattctttat tgccccactt
     8701 aattctaaaa ctaacattga taataaaact attcttaaag gctgatttgt ctttaataga
     8761 tttataaatt gtttgaaacc ttgtttttct tttttctttt ccacattatc cttaatttct
     8821 ctaataccaa aagctgtaac ccaagtaaat atagtagcaa agcaaacata tattatggcc
     8881 acagtttgcc agtttccaat tattgctaca agcggaattg ttgaagttaa aattataaaa
     8941 ttacctacat aggccactgt tcttgctgat gtaacaatct tagtttttcc tgatgaagat
     9001 cttgtaatat tggcagataa tgaccaatat ggtatatcca ttgcagtaaa ggatattccc
     9061 cagaaaatat atgctatgta tatccatatt attttaccac tataacttaa atctggtgct
     9121 gaaaaacaca taattgttgc tattcccatg aaaataggaa cgataaataa atagggtcta
     9181 ctctttccga ttttagtctt agtattatca actataaccc ccattatagg gtctgttatt
     9241 gcatctataa ttcttgccac caaaaataag gttcctatgg cagctgcccc tactttaaaa
     9301 ctatctgtta aaaagattaa aaggtaggaa ctaatcaatg cgtatatcat attatttcct
     9361 aaacttccca tactataagt aattaaagtt tttatagaaa catcttcttt tttttctact
     9421 tttttattta ttaatgtatc caaaccatca cctctatatt taaataataa aatcttataa
     9481 atacccgttt aattaaacct tcaaaaaaga aatcgatttc ttaatcttcg tttatattat
     9541 tgcaaaattt ttctattaat caaatagaat taagtttcta aaatattgat ttatatttcc
     9601 taattaattt actttttgaa attttcacac aattattttt aatatagaac tttgtaagat
     9661 ttgtttttag aaatgtattt aaaaatcaat taaataatga taaatgtatt gttaatgaag
     9721 cttaacttat taaaatataa aaaaggccgt agtttaaaac tacagcctat aacacttcaa
     9781 aattatcttc tattttgttg ttgtttctta gctcttctac aagacataca tcttgttggc
     9841 tcgttatcga atcctttttc tttgtagaat tcttgctctc ctactgaaaa tacgaattca
     9901 cttccgcaat ctctacatgt cattatttta tctgtcataa atattcctcc tgaaattaaa
     9961 gattctaaat cgatataata atctctaatt tacgagaaac tatttaccta aatgaaactt
    10021 ttttgttagc atttttttgc tactctctta tagtaacaca ctatttactt taatgcaatt
    10081 attttttaaa aataattatt aataaaaagt tttttgccat tttatattta tttaatattg
    10141 tgtaatctac actgaattca tttaataaaa tctttaatta atatataaaa aatactatac
    10201 ttaataatga caatatatta attagtttag attgaggaga tggtctttgg ttactaaaaa
    10261 tgcaatcaaa tatattctaa tattgtataa tataatcaca aaagtatgtg attatattag
    10321 taaacactat agaccaaaag agtttgcaga attattattt atacacaagt ttcaacttct
    10381 aacctaaaat gtgatttgaa aaatcaaatt gaatttctta gataatattt taatgataaa
    10441 ggaataatat tagatgaagt tattattgag gattcaagaa aaagtttaga gaggatgaag
    10501 aagttgaaaa gggcttacaa gatggagatt aatcctactg atgaacaaaa atctaaaata
    10561 caccaaacta ttggtgtatc aagatttgtc tataactttt atattgctcg taataaagaa
    10621 atttatgaga gagaaggaaa gtttgttagt ggaatggatt tttcaaagtg gttaaataat
    10681 gagtatattc ccaataatca agagatgaag tggattaaag aagtatcttc aaaagctact
    10741 aaacaagcta ttatgaatgg agataaagct tttatagatt tctttaaaaa agctaagggt
    10801 tttccaaaat ttaagaaaaa gaaaaatcaa gatgtaaaag cttattttcc taagaataat
    10861 aaaactgatt ggactttaga aagacataga gtaaaaatac caacacttgg atgggtaaga
    10921 ttaaaagaat ttggttatat tcctgtaaat ttaataatca aaagtggtac agtaagtcaa
    10981 aaagctgata gatattatgt atctatattg gttgaagaaa gagatataaa aatatctaat
    11041 tcaaatacag gtatgaaaat atctaacaca aataatgaag gcttaggaat tgatctaggt
    11101 attaaggaat ttgctgtatg tagtgatgga attaagttta a
//
LOCUS       DAEVGI010000078        11100 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEVGI010000078 DAEVGI010000000
VERSION     DAEVGI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.078"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            259..702
                     /locus_tag="K8M77_001639"
     CDS             259..702
                     /locus_tag="K8M77_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI7086133.1"
                     /translation="MNFIRDLLNENNIILKAMAFNKEEAVRLAAEPLLKDGSIREEYV
                     KDILEKINDLGPYMAIMPGVVIAHSRPGDYVFKECISMLTLENPVEFGHEDNDPIEII
                     FVIGAKENTGHLDALQDLARVLINEDSVNKIKNAQNKKEIMELFM"
     gene            877..1149
                     /locus_tag="K8M77_001640"
     CDS             877..1149
                     /locus_tag="K8M77_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI7086134.1"
                     /translation="MKIMTVCGFGVGTSLLLKMTVDSILEEEGINGYVEAIDMTSACG
                     NSADLILTSKEIGKEIEGQVSGKLVYISNFMDKEEVKEKILKAIRN"
     gene            1190..2461
                     /locus_tag="K8M77_001641"
     CDS             1190..2461
                     /locus_tag="K8M77_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBI7086135.1"
                     /translation="MKEILDFCVSFFRNPALFMGLVVAIGLILQRKPIDAILKGIFKG
                     IIGMVILLKGVDIVVSSITPLANAFSGLFNTQSNSTLGDFNVFLGQYGSYVGLILLCG
                     FVINIIIARYTRFKTIYLTGNILFWYPMLFLAVGIENNVSGLKLFIFTLIMYILVITI
                     FPYILRKHVKYVTGNDSFTIGHTASIYCLLGSYIGKLVGQKDKNIENLNLPKSLSFFR
                     DTNITAAIVMFIVYIIVGLFIGKESRTAIYGSEPLITYSLIQGITFAAGMIILLTGVR
                     MILGEIIPSFKGIADKLAKGSIPALDIPMIFPYGPNALLIGFIIALITSIGTLFLLGA
                     SGVLTFALIPLVVACYFDVAPGAIFANARGGWPAAIITSALGGIILMVLAFISLNLVS
                     GTVGNFIQTYGGNEFSIWVIIGDLVGKLFSI"
     gene            2690..3484
                     /locus_tag="K8M77_001642"
     CDS             2690..3484
                     /locus_tag="K8M77_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBI7086136.1"
                     /translation="MKNLKGIVFFDVDGTLIDCGKGLYKPSNKTKEAIKKLKDNGYLT
                     ILATGRPMSFVDDGLKELELDGYIASNGTYIELNNEVILNDDIKLEKLKEIMDYCVEH
                     NIDFILEGQKTSYVLNNDVAKKMVENFSLPEESFTGEWTEEELSANKLVIVDNNKDDF
                     KDIFERFKDEFVFMQHPGHSSYDMYRRGCTKAHGIEHLINELGLSDKDTYAFGDGEND
                     IEMFQRVKYGIAMGDAHETLIPHAYKQTENVPNEGIYKALLELGLI"
     gene            complement(3619..4065)
                     /locus_tag="K8M77_001643"
     CDS             complement(3619..4065)
                     /locus_tag="K8M77_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI7086137.1"
                     /translation="MDNIENLMGATEELYTLLLCLNRKVFSHDDIVKRLSIPPSHAKV
                     IFHLHFGGEASVSEIAKNLSISKSNMTPIIDKLIQENLVERFTDPNDRRIIRVRVTEK
                     GHEFCLKQREKTKKHLEEKISSLSLEELEKIKDLAKELNSIFSKIE"
     gene            4387..6684
                     /locus_tag="K8M77_001644"
     CDS             4387..6684
                     /locus_tag="K8M77_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086138.1"
                     /translation="MSKLIKYLKPFTASILAVIVLLFAQAMFDLSLPDYMSNIVNVGI
                     QQGGIENAVPKVISKSEFDKLTLFMSKDEKEKVESNYTLLDKGNLPKSEYDKYVKDYP
                     ALEKENIYKLNTKDKEIINELNPIMGKAMIVVGGIEEKGLQGVMGNSSLTGASDNSKA
                     NMEESAPKEGKIQGMPANTDPFFMIKNMPEAQKNDMLKQINEKFESMPESMVTQTAAT
                     FIKDQYTDLGMNIEKIQSQYILKTGGIMILLAFGSMIAAVSVTLIAARVAAGFGRNLR
                     SKVFGKVVRFSNAEFDKFSTASLITRSTNDIQQIQTLMVLLLRIVFYAPILGLGGIFR
                     VVKTDTGMSWIIAVALAAILSLVIVLFGLAIPKFKLVQKLVDKLNLVARESLTGMLVI
                     RAFNTEKYQENKFDGVNKDLTRTNLFVSRIMGGMMPLMMLIMNLITILIVWVGAHNVN
                     DGVMQVGDMMAFIQYTMQIIMAFLMISMVSVMLPRASVSGQRIAEVLETKESIKDPKK
                     NHEFNKNKKGYVEFKNVSFKYPGAEEYVLKDISFTAKPGETTAFIGGTGSGKSTLVNL
                     IPRFYDVTEGEILVDGTDIRKVSQHDLREKIGYVPQKGILFSGTIESNLKYGNEDALL
                     EELEEAANIAQASEFINTKALKYAEEISQGGTNVSGGQKQRLSIARALVKNPDVYIFD
                     DSFSALDFKTDAAVRKALNEKTSHSTMLIVAQRISTIMGANQIIVLDEGKIVGKGTHK
                     ELLENCEVYKQIALSQLSKEELSNE"
     gene            6677..8533
                     /locus_tag="K8M77_001645"
     CDS             6677..8533
                     /locus_tag="K8M77_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086139.1"
                     /translation="MSKERKGGMGGPMGRMGGGPRAVEKAKDFKGTMKKLGVYLKPYS
                     LSIAIVILFAIGSAAFSIVGPKILGKATTKIFEGLVQKITGVPDASIDFGYIGNIAMI
                     LVALYLVSSLFGIIQSFIMSGVAQKVSYNLRKQISEKMDTLPLNYFDTRTNGEVLSRI
                     TNDVDTVNQTLNQSLSQIITSVVTLIGVLIMMFSISWIMTLATFIILPVSMVLISLVV
                     KKSQKYFKSQQEYLGHLNGQVEEVYGGHNIMKAFNREEASTKDFDELNNTLYKSAWKS
                     QFLSGMMMPIMSFVGNLGYVLVSILGGWLTIKSVITVGDIQAFIQYVRSFNQPIAQMA
                     QVANIMQSTAAAAERVFEFLDEEDEVKDPVNSVDPSEIRGEVEFEDVHFGYNEDKIII
                     NDFSVDVKPGQKVAIVGPTGAGKTTIVKLLMRFYDINSGSIKIDGHDIRDFKRADLRN
                     LFGMVLQDTWLFNGTIMENLRYGRLDATDAEVKEAAKAAHVDHFVKTLPDGYNMVLNE
                     EASNISQGQKQLLTIARAFLKDPKLLILDEATSSVDTRTELLIQKAMEKLMEGRTSFI
                     IAHRLSTIRDADLILVMKDGDIVEQGNHEELLEKGGFYSSLYNSQFEQSSAS"
     gene            8929..9810
                     /locus_tag="K8M77_001646"
     CDS             8929..9810
                     /locus_tag="K8M77_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086140.1"
                     /translation="MKFRFLYVGDKILSHREEGLFRVLNCEEYSLNKKVLDYKEICNR
                     KKYKNGFSEEKFNRIYLSKRGSIINFYSLESKEFSETYLNDINRFKDLLDLNKNSENE
                     EIKIIFFDLFNKDANKDQLFITSFMNKLGEISKDINWDNILIIFNEEIIFNKATDINS
                     RFRECSLVLNNYDVNLNEFIHILIDFLLLRNDLGDKWAEYRKKIANSKLTLGKGKGEN
                     FSGEVIMRILGEFSKIQRDNLFKLILMRKEEINLNEIALIEDALKEYISLDSRLIIKQ
                     LTRDFFDDEVRYILFSK"
     gene            10065..>11100
                     /locus_tag="K8M77_001647"
     CDS             10065..>11100
                     /locus_tag="K8M77_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBI7086141.1"
                     /translation="MLEIIQKTLTDNAIIGAIFSSIAIIFLGFYIRKRNILTGAASKI
                     LTKVVLTVAIPALAFTSFMKDINQKELHEGINILIWGFVIYILLIVLTKFIFARVKGD
                     KQDVFRVLTIFGSTTFFGVPIVKAVYGSVGAMYSSIFNIAYRVFLYSYGYIKMSGVKM
                     DKENMKKNLKEMLLNPVIIATFLGLFIWLFQGMLPQVQIGDKSYAFLRIDQTLPWLYK
                     PLTYLDKLCSPLAWLAIGITLAEVPFKQAVGQKEPWVYSLIKIILVPVINLGLLVVLN
                     KTGILPVSFEGMATTVIMLATPTATVAAAYAISFDKEALLTSNCSLLSTVVAVVAMPF
                     WIVILQIIKNI"
BASE COUNT         4219 a         1170 c         1928 g         3783 t
ORIGIN      
        1 aaattttatt ttatagagat atattaaatt taaaatgtta agtggttatg tctttaaaaa
       61 tcctttgaat tatgaaatat ttttcttcta attataaatt tttatatttt gtatttaaaa
      121 ggattatttt acttaaatat actttaaaat tgactttaaa tgctttggaa tgataatata
      181 cttaggtaaa tttaacatgc ttgtaaagta attaggggtt tttgtttgta tgatgttaaa
      241 ttaacgtaag gaaagttggt gaattttatt agggatttat taaatgagaa taatataatt
      301 ctcaaggcta tggcttttaa caaagaagag gcagttagat tggctgcaga gcctctttta
      361 aaagatggaa gtataagaga ggaatatgta aaagacattt tagagaaaat caatgactta
      421 ggaccataca tggcaattat gccaggagtg gttattgctc attctagacc aggtgattat
      481 gtgtttaaag agtgtattag catgcttact ttagaaaatc cagttgaatt tggtcatgag
      541 gataatgatc caatagaaat aatttttgtt attggagcaa aggaaaacac aggtcattta
      601 gatgctctac aagatttagc aagggtctta ataaatgagg attctgttaa taaaattaaa
      661 aatgctcaaa ataaaaaaga gataatggag ctttttatgt aatttattaa acttttcaaa
      721 aagtaactta taagatattt taaattagga tattttataa gtaatattta tttgtagata
      781 aattttaaga gatgaattga cttctaaggg ataagtatta aaaggatctt ttgaaattta
      841 aatctatcta tatactatgg agaggagggg atataggtga aaattatgac tgtatgtgga
      901 tttggagttg gaacaagttt acttttaaaa atgacagttg attcaattct tgaagaggaa
      961 ggaataaatg gttatgttga agctattgat atgacttcag catgtggaaa ttctgcggac
     1021 ttaattttaa catcaaaaga aattggaaaa gagatagaag ggcaagtaag tggaaaactt
     1081 gtttatatat caaactttat ggataaagaa gaagtaaagg aaaagatttt aaaagctata
     1141 agaaattaag aaaaattaaa ttagattaaa taaaaaggaa ttattaaaaa tgaaagaaat
     1201 attagatttt tgtgtaagtt tttttagaaa tccagcttta tttatggggc ttgtagtagc
     1261 aattggttta atacttcaaa gaaaaccaat agatgctatt ttaaaaggca tatttaaagg
     1321 aataatagga atggtaattc tacttaaggg agtagatata gtagtttctt caattactcc
     1381 tttagctaat gccttttcag gattatttaa cactcaaagt aatagtacat taggagattt
     1441 taatgtattt ttaggtcagt atggaagcta tgttggatta atacttcttt gtggatttgt
     1501 aatcaacata atcattgcta gatatacaag atttaagact atatacttaa caggaaatat
     1561 attattttgg tatccaatgc ttttcttagc agttggtatt gaaaataatg taagtggatt
     1621 aaagttattc atattcacat taattatgta tattttagtt ataacaatat tcccatatat
     1681 attaagaaaa catgttaagt atgtaactgg aaatgatagt tttaccattg gacatactgc
     1741 ctcaatatat tgtttattag gttcttatat aggaaaactt gttggacaaa aagataaaaa
     1801 tatagaaaat ttaaatcttc caaagtcttt aagtttcttt agagatacaa atataacagc
     1861 ggcaatagtt atgtttatag tttatataat agttggttta tttataggaa aagaaagtag
     1921 aacagctata tatggaagtg agccacttat tacttattct ttaattcaag gaataacttt
     1981 tgcagcaggt atgataatac ttttaacagg ggtaagaatg attttaggag aaataatacc
     2041 atcatttaaa ggtatagcag ataaattagc gaagggttca attcctgctt tagatattcc
     2101 tatgatattc ccatatggac caaatgcttt attaattgga ttcataatag cacttataac
     2161 aagtataggt actttattct tattaggagc atcaggggta ttaacctttg ctcttatacc
     2221 actagtagtt gcatgttatt ttgatgttgc cccaggagct atctttgcaa atgcaagagg
     2281 gggatggcca gcagctataa ttacaagtgc cttaggagga ataatcctaa tggtattagc
     2341 atttatttcc ttaaatttag taagtggaac tgtaggaaac ttcattcaaa catatggagg
     2401 aaatgaattc tcaatatggg taataatagg tgacttagta ggaaagcttt ttagcatata
     2461 ataaagcagt ttaatttaga gactaatata taatatttat tataggtaac aaataaatag
     2521 atctttataa aacttacaaa cagtatgaag agaaatctag taattctctg aatatgtttg
     2581 taagttttat ttttatacag tgttacattt catttaatta tgttgaaaag atttataatc
     2641 tgaaaaaaag tatgttatca tagtaattat taaaaggagg aggggactta tgaagaattt
     2701 aaaaggaata gtattttttg atgtggatgg tacattaatt gattgtggca aagggttata
     2761 taaaccttca aataaaacta aagaggccat taaaaaatta aaagacaatg gatatttaac
     2821 aattcttgca acaggacgtc caatgagctt tgttgatgat ggattaaagg aattagagtt
     2881 agatgggtat atagcatcta atggaacata tatagaatta aataatgaag tcattcttaa
     2941 tgatgatata aaattagaaa agttaaaaga gataatggat tattgtgttg aacataatat
     3001 tgactttatc ttagaaggac aaaagacaag ttatgtttta aataatgatg tggctaaaaa
     3061 aatggtagaa aatttttctc ttccagagga aagttttact ggtgagtgga cagaagagga
     3121 gctttcagca aataagcttg ttattgttga taataataag gatgatttta aagacatatt
     3181 tgaaagattt aaggatgaat ttgtatttat gcaacatcca ggacattcat cttatgatat
     3241 gtatagaaga ggatgtacaa aggctcatgg aatagaacat ttaataaatg agctaggctt
     3301 aagtgataag gatacttacg cctttggaga tggagaaaat gatatagaaa tgttccaaag
     3361 agttaaatat ggaatagcaa tgggagatgc ccatgaaact ttaataccac atgcatataa
     3421 gcaaacagaa aatgttccaa atgaaggaat atataaggct ttattagaat taggcttaat
     3481 ataataaaaa attatgaagt acgtgtataa aaataacttt tgataaaact tacaaacagt
     3541 atgaagagaa atctagtaat tctcggaata tgtttgtaag ttttattttt atattttgat
     3601 atagctctag cagtcacttt attctatttt agaaaaaatg ctattaagtt ctttggctaa
     3661 atctttaatt ttttctaatt cctctaatga aagggatgat attttttctt caagatgctt
     3721 tttagttttt tctctttgtt ttaaacaaaa ttcatgccct ttttcagtta ctctaactct
     3781 gatgattctt ctatcattag gatctgtaaa tctttcaact aaattttctt gaataagctt
     3841 atctataatt ggagtcatat ttgattttga tatagataaa tttttggcta tttctgaaac
     3901 agaagcttcc ccaccaaaat gtaaatgaaa tataactttg gcatgtgatg gaggaataga
     3961 caatctttta acaatatcat catgactaaa tacttttcta tttaaacaca ataataatgt
     4021 gtaaagttct tcagtagctc ccattaaatt ttctatgtta tccatttttt acctcctatt
     4081 tattaagttt ataaatttta gttgatgtat aatataataa atgaataaat tttaaagttt
     4141 ttagtatcgt agtaaaacac tttatattat aaccatatat aactattata caccagaaat
     4201 agttattgac atagaatagt tataaaaata taattaatat taaaatataa ctatttataa
     4261 agggatagat ttgaaaactt tgttttagtg agtatttatt tttaaacata tttttagaat
     4321 taacttaata tcaaagacaa tacaaattta aaacattata aattaaaaaa gaaaagaggg
     4381 aaatacatgt caaagctaat taaatactta aaaccattta cggcatcaat tttagctgtt
     4441 atagtacttc tttttgctca ggcaatgttt gatttatctt taccagatta catgtcaaac
     4501 atagtcaatg taggaattca gcaaggtggt atagaaaatg ctgttccaaa agtaattagt
     4561 aaatcagagt ttgataaact gacacttttt atgagcaaag atgaaaaaga aaaagtagaa
     4621 agtaattata cgttgttaga taaaggtaac cttcctaaaa gtgagtatga taaatatgta
     4681 aaagattatc ctgctttaga gaaggagaat atttacaaat taaatactaa ggataaagag
     4741 ataattaatg aattaaatcc tattatgggt aaggcaatga tagttgtagg tggaattgaa
     4801 gaaaagggcc tacaaggagt aatgggaaat tcatctctta caggagcttc tgataattcc
     4861 aaggctaata tggaagaaag tgctcctaaa gaaggaaaaa tacaaggaat gcctgctaat
     4921 acagatccat tttttatgat taaaaatatg ccagaggcac aaaagaatga tatgcttaag
     4981 caaattaatg agaaatttga aagtatgcca gagagtatgg ttactcaaac agcagccact
     5041 tttattaaag atcaatatac tgatttaggc atgaatatag agaagataca aagccaatac
     5101 atactaaaaa ctggaggaat aatgattctt ttagcctttg gaagtatgat tgcagcagta
     5161 tcagtaacat taatagccgc gagagtggca gcaggatttg gaagaaatct tcgtagtaaa
     5221 gtttttggaa aagttgttag attttcaaat gcagaatttg ataaattttc aacagcttcc
     5281 cttataactc gtagtacaaa tgatattcaa caaatacaaa ctcttatggt tctacttctt
     5341 agaatagttt tctatgcacc tatcttaggt ctaggaggta tttttagagt tgtaaaaaca
     5401 gatactggaa tgtcatggat aatagctgta gccctagctg ctatactttc tttagtaata
     5461 gttttatttg gacttgctat tccaaaattc aagttagttc aaaagcttgt tgataaactt
     5521 aacttagtag ctcgtgaatc acttacagga atgttagtaa tccgtgcctt taatacagaa
     5581 aaatatcaag agaataagtt tgatggtgta aataaagatc ttactagaac aaatcttttt
     5641 gttagtagaa taatgggtgg aatgatgcca cttatgatgc ttataatgaa ccttataact
     5701 attcttatag tatgggttgg tgctcataat gtaaatgatg gagttatgca agttggggat
     5761 atgatggcat ttattcaata cacaatgcaa atcataatgg catttttaat gatatctatg
     5821 gtatcagtaa tgcttccaag agcatcagtt tcaggtcaac gtatagctga ggttttagaa
     5881 actaaagaaa gcataaaaga ccctaaaaag aatcatgagt ttaataaaaa taaaaaagga
     5941 tatgttgaat ttaaaaatgt ttcatttaaa tatcctggtg cagaggaata tgttttaaaa
     6001 gatatttcat ttactgcaaa gcctggggaa acaactgcct tcattggagg aactggtagt
     6061 ggtaaatcta ctttagttaa tttaatacca cgtttctatg atgttacaga aggtgaaatc
     6121 ttagttgatg gaacagatat aagaaaagtt tctcaacatg atttaagaga aaaaataggt
     6181 tatgttcctc aaaagggaat attattctca ggtactatag aaagtaattt aaaatatggt
     6241 aatgaggatg cattattaga agaattagaa gaggctgcaa atattgctca agcttctgaa
     6301 tttataaata ctaaagcttt aaaatatgca gaggaaattt cacaaggtgg tactaacgta
     6361 tctggtggac aaaaacaaag actttcaatt gctagagctt tagtaaaaaa tcctgatgta
     6421 tatatttttg atgatagttt ctcagctctt gactttaaaa cagatgcagc tgttagaaaa
     6481 gctcttaatg agaaaacatc acatagcaca atgcttatag ttgcacaaag aataagcaca
     6541 attatgggag ctaatcaaat aatcgtactt gatgaaggta agattgttgg taagggaact
     6601 cataaagaac ttctagaaaa ttgtgaggtt tataagcaaa tagctttatc tcaactttca
     6661 aaggaggagc taagtaatga gtaaagaaag aaaaggcgga atgggtggcc caatgggtag
     6721 aatgggcgga ggcccaagag ccgttgaaaa agcaaaagat tttaaaggaa caatgaaaaa
     6781 gcttggggtt tatttaaaac cctatagttt atctatagct attgtaatat tatttgctat
     6841 aggaagcgca gccttctcaa tagttggacc taagatttta ggtaaagcta ctactaaaat
     6901 atttgaaggt ttagttcaaa agataacagg ggtaccagat gcttctatag attttggata
     6961 cattggaaat atagcaatga ttttagtagc tctatactta gtaagttcat tatttgggat
     7021 tatacaatct tttataatgt caggagttgc tcaaaaggtt tcttataatt taagaaagca
     7081 aatatcagaa aaaatggata ctttacctct taattatttt gatacaagaa caaacggaga
     7141 ggttttatca agaattacaa atgacgttga tacagtgaac caaactttaa atcaaagttt
     7201 atcacaaata ataacttcag tggttacttt aattggggta ttaataatga tgttctcaat
     7261 aagttggatt atgactttag caacatttat aatacttcca gtttcaatgg ttcttatatc
     7321 tttagttgtt aagaaatctc aaaaatactt taaatctcaa caagaatact taggtcatct
     7381 taatggtcaa gtagaagagg tttatggtgg acacaatata atgaaagctt tcaatagaga
     7441 agaagcttca actaaagact ttgatgaatt aaataacact ttatataaat cagcttggaa
     7501 atctcaattc ttatcaggta tgatgatgcc aattatgtct tttgtaggaa acttaggata
     7561 tgttttagtt tcaatacttg gtggatggtt aacaattaaa agtgttataa cagttgggga
     7621 tatacaagca tttatccaat atgtacgttc atttaaccaa ccaatagctc aaatggctca
     7681 ggttgcaaat ataatgcaat caacagcggc ggctgctgag agagtatttg aattcttaga
     7741 tgaagaggat gaggttaaag atccagttaa ttcagtagat ccttcagaaa taagaggaga
     7801 agttgaattt gaagatgttc actttggata taatgaagat aagataataa taaatgattt
     7861 ctcagtagat gttaaaccag ggcaaaaggt ggctattgtt ggacctacag gagcaggtaa
     7921 aactacaata gttaaattat taatgagatt ctacgatata aatagtggaa gtattaagat
     7981 tgatggccat gatataagag actttaagcg tgctgattta cgtaacttat ttggtatggt
     8041 tcttcaagat acatggttat ttaacggaac tataatggag aatttacgtt atggaagact
     8101 agatgcaaca gatgctgagg ttaaagaagc tgctaaggct gcccatgttg atcatttcgt
     8161 aaaaacttta ccagatggat ataatatggt tttaaacgaa gaagcaagta atatatcaca
     8221 aggacaaaag cagcttttaa caattgctag agctttctta aaagatccta agctattaat
     8281 acttgatgag gctacaagtt cagttgacac tcgtacagag cttttaatac aaaaagctat
     8341 ggaaaaactt atggaaggaa gaacaagctt tattatagct cacagacttt ctactatacg
     8401 tgatgcagac ttaatacttg ttatgaagga tggagatata gttgaacaag gaaatcatga
     8461 agagcttcta gaaaaaggtg gattctattc aagcctttac aatagccaat ttgaacaaag
     8521 tagtgcttca taagaaatat aaactttaaa atttaagatt atgagatttt aaaataagtt
     8581 attttaaatt agagttttat agaagtattt aataaattat ttgggttaaa tagtgttaaa
     8641 aagtaatttt tcttaaaaat attataaata aaatatatga ataatgtagc tgatatgaat
     8701 tttaagaatt ttaaatttgt atcagctata ttttttattt taagataact ttttaaagat
     8761 ttagtttgat agatataatt taatttacta gagaatgcaa agaatctaat atgatataat
     8821 tatatgtagc taagataaaa taattattaa tagcttttat tgcctaggtt tttattaaat
     8881 aattttagtg aaaatacggt attaaaagtg aattttgtga ggttttttat gaagtttagg
     8941 tttttatatg ttggtgataa aatcttaagt catagagaag aggggctttt tagggtttta
     9001 aattgtgagg aatattcttt aaataaaaag gttttagatt ataaagaaat ttgtaataga
     9061 aaaaaatata aaaatggatt tagtgaagag aagtttaata ggatttatct aagtaaaaga
     9121 ggttctataa ttaactttta ttcattagaa agcaaagaat ttagtgaaac ttatttaaat
     9181 gatataaata ggtttaagga tttacttgat ttaaataaaa atagtgaaaa tgaagagatt
     9241 aaaataattt tttttgattt atttaataaa gatgctaata aagatcaatt gtttataact
     9301 tcattcatga ataaattagg tgaaatttca aaagatataa attgggacaa tatattaatc
     9361 atatttaatg aagagattat atttaataaa gctactgata taaattcaag atttagagag
     9421 tgctctttag tattaaataa ttatgatgtt aatttaaatg agtttattca tattttaata
     9481 gatttcctat tattaaggaa tgatttagga gataaatggg ctgaatatag aaaaaaaata
     9541 gctaactcta aattaactct tggaaagggg aagggagaga atttttcagg agaggtaatt
     9601 atgaggattt taggtgagtt ttctaaaata cagagagata atttatttaa attaatatta
     9661 atgagaaaag aagaaataaa tttaaatgag atagctctta tagaggatgc tttaaaagag
     9721 tatatatctt tagattctag gttgataatt aaacaattaa caagagattt ttttgatgat
     9781 gaagtaagat acatactttt ttctaaataa aaagaataat gattttatag ttgaaagttc
     9841 ttaaaatggc ttataaagcc gttttgaggg ctttttttag tttttaataa attttagttt
     9901 tgttaattat ttaacttaaa taacttaaat aattatttaa aaacttaaat aaattgtttg
     9961 aaaagagcaa ggcttgtaca ataattcttg taagaagaaa tttcgacaaa aatcaacata
    10021 actttaaatg ttaaaaaaaa cacaaaatag gaggaaggtt agtaatgtta gaaatcattc
    10081 aaaaaacact tactgataat gcaatcatag gtgcaatatt ttcatcaata gcaattatat
    10141 tcttaggatt ttatattaga aaaagaaata tattaacagg ggctgcatct aaaatattaa
    10201 ctaaggttgt attaacagtt gcaattccag cattagcatt tacttcattt atgaaagata
    10261 ttaatcaaaa agaattacat gaaggtatta atattctaat atggggattt gtaatttata
    10321 tattattaat tgtattaaca aagtttattt ttgcaagagt taaaggtgac aagcaagacg
    10381 tatttagagt acttactata tttggatcaa caactttctt tggtgttcca atagttaaag
    10441 cagtttatgg ttcagttgga gctatgtact catcaatatt taacatagca tatagagttt
    10501 tcttatattc atatggatac atcaaaatgt caggtgtaaa aatggacaaa gaaaatatga
    10561 agaaaaattt aaaagaaatg ttattaaacc cagtaattat agctacattc ttaggattat
    10621 ttatatggtt attccaaggt atgttaccac aagttcaaat aggtgataag agttatgcat
    10681 tcttaagaat agaccaaaca ttaccttggt tatataaacc acttacatac ttagataaat
    10741 tatgttcacc acttgcatgg ttagctatag gtattacttt agcagaagta ccatttaagc
    10801 aagctgtagg acaaaaagaa ccttgggttt acagtttaat aaaaataata ttagttccag
    10861 ttataaactt aggattatta gtagtattaa ataaaacagg tatattacca gtttcatttg
    10921 aaggaatggc tactacagtt atcatgttag ctactccaac tgctacagtt gctgcagctt
    10981 acgcaataag ttttgataag gaagctttat taacatcaaa ctgttcatta ctatcaacag
    11041 ttgttgccgt agtagcaatg ccattctgga tagtaatatt acaaataatc aaaaacatag
//
LOCUS       DAEVGI010000079        11096 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEVGI010000079 DAEVGI010000000
VERSION     DAEVGI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.079"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            18..1070
                     /locus_tag="K8M77_001648"
     CDS             18..1070
                     /locus_tag="K8M77_001648"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBI7086142.1"
                     /translation="MKVLRVNLDEKSYDIVIQKDLKDYFGEYIKTVFDGKKVAIITDD
                     NLNDIYGEAIKKNIESEGFEVEVISVTPGEKSKSFSILPGIYNKLLDFKLTRSDLIIA
                     LGGGVVGDLAGFVASTFLRGISFIQIPTSLLAQVDSSVGGKVAVDLERGKNLVGSFYH
                     PQLVLIDPNMLGTLPEKYFNDGLGEVIKYGCIKSKELFEKLEGFKNKEDLKENIGEII
                     YECCNIKRELVENDEKDLGERMILNFGHTLGHAIEQIYNYETYSHGEAVAIGMNMISK
                     IAEEKDLTKKGTAERIESLLKKYGLNTDVNIEDNGLAREAIKLDKKNLNGNLNVILLK
                     DIGEGYIYNTTVEFFE"
     gene            1093..2367
                     /gene="aroA"
                     /locus_tag="K8M77_001649"
     CDS             1093..2367
                     /gene="aroA"
                     /locus_tag="K8M77_001649"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBI7086143.1"
                     /translation="MKKVIITPSKLRGSVKIPPSKSMAHRAIICASLSKGESVISNID
                     FSEDIIATMEGMKSLGANIKVEKDKLIINGENILKDSNYKVIDCNESGSTLRFLVPIS
                     LIKDNKVNFIGRGNLGKRPLKTYYEIFEEQEIKYSYEEENLDLNIEGSLKGGEFKVKG
                     NISSQFISGLLFTLPLLKDDSKIIITTELESKGYIDLTLDMIEKFGVTIKNNNYREFL
                     IKGNQSYKPMNYKVEGDYSQAAFYFSAGALGSEINCLDLDLSSYQGDKECIEILEGMG
                     ARLIKNQEESLSIIHGDLNGTIIDASQCPDIIPVLTVVAALSKGETSIINGERLRIKE
                     CDRLNAICTELNKLGADIKELKDGLIINGVKELIGGEVYSHKDHRIAMSLAIASTRCK
                     EEVIIREPDCVKKSYPGFWEDFKSLSGILREE"
     gene            2431..3504
                     /gene="aroC"
                     /locus_tag="K8M77_001650"
     CDS             2431..3504
                     /gene="aroC"
                     /locus_tag="K8M77_001650"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBI7086144.1"
                     /translation="MGGVWGNKIKLSIFGESHGEGIGIVIDGIEPGIKINMDNIEKDM
                     ERRAPGRNSLSTQRKEGDKPEILSGIFNGITTGAPISMIIRNTDKRSRDYSKIKDVMR
                     PGHADFPGYIRYNGFNDYRGGGHFSGRITAPLVFAGALAKEMLKEKGITIGSHIKQVG
                     KVKDSSFDALNLKKEDLEELLTKELPVIDANKIEEIKEEITSYRMEGDSIGGIVECAI
                     VGLEAGIGNPFFDSLESTIAHLAFSVPAVKGIEFGEGFDFANMKGSEANDEYFIEDEK
                     VKTYSNNNGGITGGISNGMPVIFRVVIKPTPSISKEQRTINIKNMTEEVLSVNGRHDP
                     CIVQRALVVIEAIAAISILELIK"
     gene            3674..3967
                     /locus_tag="K8M77_001651"
     CDS             3674..3967
                     /locus_tag="K8M77_001651"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBI7086145.1"
                     /translation="MSDKLAQCRKEINRIDGELMKLFEERMNVVLDVARYKKENNMGI
                     FHKDRENQVIEKNLSRVKNKELLPYAEEMLHALMDISKKYQQYKIEMRENFIK"
     gene            4787..5602
                     /gene="aroE"
                     /locus_tag="K8M77_001652"
     CDS             4787..5602
                     /gene="aroE"
                     /locus_tag="K8M77_001652"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBI7086146.1"
                     /translation="MKLFGLIGEKLGHSLSPEIHNKVFKDNNIDGLYNLFSVKKDFEN
                     NIVESLKCLGVRGANVTIPYKEKVMDQLDIISHEAKAIGAVNTILIKDGKSYGYNTDY
                     YGFGKMLERAKVDIEGNSFFVLGAGGAARSILKYLEDSKAKKIVLVSRDKEKVFKKFK
                     DFNINFMSYGELEEIHEEFALINTTPCGMYPNTNSVAVSEKVIKKFKVALDIVYNPLE
                     TKFLKMAKDNGLKTVDGLFMLVGQGVKAEEIWNGIKVDKSTEENIYEELKCRF"
     gene            5765..6268
                     /locus_tag="K8M77_001653"
     CDS             5765..6268
                     /locus_tag="K8M77_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBI7086147.1"
                     /translation="MKKRGVLLIGMPGAGKTTIGRELSTVLKMNFLDMDEFIERSTGK
                     EIKELFAQGEEVFRDLESKSCELLSTLKNVVISSGGGIVTREENMGNFKEFITVFINR
                     PLDLIMEDIDTEGRPLLWDGKERLTNLYRDRIDLYKKHSDIEIINDGTTKEAIREIVE
                     AISELAY"
     gene            6335..6769
                     /gene="aroQ"
                     /locus_tag="K8M77_001654"
     CDS             6335..6769
                     /gene="aroQ"
                     /locus_tag="K8M77_001654"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HBI7086148.1"
                     /translation="MKIMVINGPNLNLLGIREKEIYGAKDFNQVIDYIKEEGKELGLE
                     VNCFQSNIEGEIINFIHNAYFEKYDGIIINPGAYTHYSIAIYDALKGVEIPTVEVHLS
                     NIHKREEFRHKSVTAPACIGQISGFGEYGYIMAMNALKNKIK"
     gene            complement(6790..7131)
                     /locus_tag="K8M77_001655"
     CDS             complement(6790..7131)
                     /locus_tag="K8M77_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086149.1"
                     /translation="MLKYKDKNYVCLLDLAMELIRGKWKAVILCHLNKGKKRFLELQR
                     ITCGISHKVLTEKLKELEEDELVKKIVYDENPPRVEYYLSEKGKELAPVLECLEDWSR
                     KYFGNLETPSK"
     gene            7387..8493
                     /locus_tag="K8M77_001656"
     CDS             7387..8493
                     /locus_tag="K8M77_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBI7086150.1"
                     /translation="MKTLFDKTNLGTINMKNRFLRGALWTALADEKGHMTNELSDVYE
                     ELARGGVGTIVTGYAFVTENEQPNPRMMGIYDDSFIPEYKEFTDKIHRLGSNIIMQIV
                     YGGFMTEFNVGERVIWGPSTTKNEVTGTLSKEMTKEDIKYLVNAYAEAALRVKKSGFD
                     GVEIHGGHGYLVSQFLSPYYNKRTDEYGGSIENRGRFVFEIFKAIREKVGEKFPILIK
                     LNSSDYVKEGGLTIEDSLYVAERLAELGIDAIEVTGGNESIEEVMKNNLGPSRTKVAI
                     SKDRESYFKEYAIKLAERINKPVILSGGNRHFDVMEELLNDTNIEYFSLSRPLTAEPD
                     LINKWYSGDLKKPKCKSCNQCYKTYGKRCIFNIK"
     gene            9230..9745
                     /locus_tag="K8M77_001657"
     CDS             9230..9745
                     /locus_tag="K8M77_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086151.1"
                     /translation="MKERIENLQRIIKNILSSTDDQIQGDLRDSLRLRMSVSENLHGE
                     VRYLKCLRALVEEKFQEFFKKKNFPKDYNDFVGIWSSLSEEAKSLCNDPKYDYDEEKY
                     KYEFIYFEVYCNVYLRYSLGLLFNGNNKDTIDDLSQYNSLEIIQMYRYYLHQITLKKL
                     EKSLKSDKVNS"
     gene            9871..10203
                     /locus_tag="K8M77_001658"
     CDS             9871..10203
                     /locus_tag="K8M77_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBI7086152.1"
                     /translation="MELNKEIIKGHVESIILSLLQGKDLYGYEISKLIRENSKEAFEV
                     KDGTLYVVLKRLEKLGLVISYWGDCKSGGGRRKYFKITEDGVNYLKEKKSEWEFLKSI
                     MNIFLGGE"
     gene            10205..11062
                     /locus_tag="K8M77_001659"
     CDS             10205..11062
                     /locus_tag="K8M77_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086153.1"
                     /translation="MREIDNYIDSLYKNIDGISKENKEIKETMRTHLIESVEELKLEG
                     FSEKESIKIALERFGEINEIRGELKTVVERGVKPLLYTVGISLIILIAFFTHLIFMRF
                     DYIKLLALIIIAVPMYIIIRGGVLIYYKVKGIKYDIDWKKELYKFLFAIYFIFLVGWY
                     IFPIEVGGSSNVNFQVDLIPFKSYIELMNRGITIGYLMKWIIIRLILFIPLGFYPAFL
                     KGKFNNIAGCVLIASIVYFIVPILELILSFGLISVPIIYIGIDYWVISIIGVLLGYFL
                     YNSTAKNYK"
BASE COUNT         4473 a          925 c         2046 g         3652 t
ORIGIN      
        1 ggtttggagt gataaaaatg aaagttttaa gagtaaattt agatgaaaaa agttatgata
       61 tagtaattca aaaggattta aaagattatt ttggagaata cataaaaact gtatttgatg
      121 ggaaaaaagt agctattata actgatgata atttaaatga tatatatgga gaggctataa
      181 agaaaaatat agaaagtgaa ggttttgaag ttgaagttat ttctgttaca ccaggagaaa
      241 agagtaaatc ttttagtata ttaccaggaa tatataataa acttttagat tttaaactaa
      301 ctagaagtga tttaatcata gctctaggtg gcggtgttgt tggagacctt gcaggttttg
      361 ttgcctcaac ttttttaaga ggaattagct ttatacaaat acctacatca ttattagctc
      421 aagttgattc tagcgttggt ggcaaggtag ctgttgactt agaaagaggt aagaatttag
      481 taggaagctt ttatcatcca caattagtat taatagatcc aaatatgctt ggaacacttc
      541 cagaaaaata ttttaatgat ggccttggag aggtaataaa atatggatgc attaagagta
      601 aagagttatt tgaaaagtta gagggcttta aaaataaaga ggatctaaaa gagaatattg
      661 gagaaattat ttatgaatgc tgcaatataa aaagagaatt ggttgaaaat gatgaaaaag
      721 atttaggaga aagaatgatt ctaaactttg gtcatacttt aggtcatgcc atagagcaaa
      781 tttataatta tgaaacttat tctcatggag aagctgttgc tataggaatg aatatgataa
      841 gtaagattgc agaagaaaag gatcttacta aaaaaggaac agcagaaagg attgagagtt
      901 tattaaagaa gtatggatta aatactgatg ttaacattga agataatgga ttagctagag
      961 aagctataaa attagataag aaaaatttaa atggaaattt aaatgttatt cttttaaaag
     1021 atattgggga aggatatata tataatacaa ctgtagaatt ttttgaataa aggcataggg
     1081 agctggtaaa aagtgaaaaa ggtaattata actcctagta agttaagggg aagtgtaaaa
     1141 ataccacctt ctaaaagtat ggctcataga gctattattt gtgcttcttt aagcaaagga
     1201 gaaagtgtta tttctaacat agatttttca gaagatatta ttgcaactat ggaaggaatg
     1261 aaatctttag gagcaaatat aaaagtagaa aaagataaac taattataaa tggagaaaat
     1321 attttaaagg attctaatta taaagttatt gattgtaatg aatcaggttc cactttaaga
     1381 tttttagttc caatttcctt aataaaagat aataaagtta attttatcgg tagaggaaat
     1441 ttaggaaaaa gaccattaaa aacttattat gagatttttg aggagcaaga aattaagtat
     1501 tcctatgagg aagaaaatct tgatttgaat atagaaggaa gcttaaaagg tggagaattc
     1561 aaagttaagg gaaatataag ttctcaattt ataagtggtt tattatttac tcttccttta
     1621 ttaaaagatg attctaaaat aataataact acagaacttg aatctaaagg atatatagat
     1681 ttaactttag acatgataga aaagtttgga gttacaataa aaaataataa ttatagagaa
     1741 tttttaataa aaggtaatca aagttataag cctatgaatt ataaggttga aggtgattac
     1801 tcacaggctg ctttctattt ttcagcaggg gccttaggct cagaaataaa ttgtcttgat
     1861 ttagatttaa gttcttatca aggggataag gaatgcattg aaatattaga gggtatgggt
     1921 gctaggctta taaaaaatca agaagagtct ttaagtataa ttcatgggga tttaaatgga
     1981 acaattatag atgcttcaca gtgcccagat ataattccag ttttgacagt ggttgctgct
     2041 ttaagtaaag gagagactag tattataaac ggagaaagac ttagaataaa agaatgtgat
     2101 agattaaatg ctatatgcac tgagcttaat aaactaggtg cagatataaa ggaattaaaa
     2161 gatggactta taataaatgg agttaaagag ttaataggag gagaagtata tagccataaa
     2221 gatcatagaa tagctatgag tttagctatt gcttctacaa gatgcaagga agaggttatt
     2281 ataagagaac cagattgtgt taaaaaatct tatccaggat tttgggaaga ttttaagagc
     2341 ttaagtggaa ttttaagaga agaataatat ataaaatatt tgacagaaag gaggaagctt
     2401 ttagagataa tatgctttaa aagcaaggaa atgggaggag tttggggtaa taaaataaaa
     2461 ttatctatat ttggagaatc tcatggagaa ggaataggaa tagtaataga tggaatagaa
     2521 cctggaataa aaataaatat ggataacata gaaaaagata tggaaagaag agcaccagga
     2581 agaaatagtt tatcaactca aagaaaagaa ggggataaac cagaaatttt aagtggaata
     2641 tttaatggaa tcaccacagg ggctcctatt tcaatgataa taagaaatac agataaaaga
     2701 tctagggatt attcaaaaat aaaagatgta atgagaccag gccatgcaga tttcccagga
     2761 tatataagat ataatggctt taatgattat agagggggag gacatttctc aggaagaata
     2821 acagcgcctt tagtttttgc tggagcctta gctaaggaaa tgcttaagga aaaaggtata
     2881 actattggtt ctcatattaa gcaagttgga aaagttaagg attcttcctt tgatgcatta
     2941 aatttaaaga aagaagattt agaagaactt ttaactaaag aacttccagt aatagatgca
     3001 aataaaatag aagaaattaa ggaagagatt acttcatata gaatggaagg agattctatt
     3061 ggaggaattg ttgagtgtgc catagtagga ctagaggctg gtataggaaa tccattcttt
     3121 gattctttag aaagtaccat agctcattta gctttttcag tgcctgctgt aaagggaatt
     3181 gaatttggag aaggttttga ctttgcaaat atgaaaggat cagaagcaaa tgacgaatat
     3241 ttcatagaag atgaaaaagt taagacatac tctaataata atggaggaat aactggtgga
     3301 atatcaaatg gaatgccagt tatattcaga gttgttataa aacctacacc atctatttct
     3361 aaagaacaaa gaactataaa tataaaaaat atgacagagg aagttctaag tgtaaatggt
     3421 agacatgatc cttgtatagt tcaaagagcc ttagttgtta tagaggccat tgcagctatt
     3481 tctatattag agttaataaa ataaaaagtt taaagtatag tagactttta tgataatagg
     3541 atattaatta aagttaatct ttgtctacta tattttaaaa tatttcaaag tgattcattt
     3601 tatcaaagtt ctagattaaa ttaatattta cttaaaaaag attaaagaag ttaaaagaaa
     3661 agaggtgaaa tttttgagtg acaagttagc tcaatgtagg aaagaaatca atagaataga
     3721 tggggaactt atgaaacttt ttgaagaaag aatgaatgtt gttttagatg ttgcaagata
     3781 caaaaaagaa aataatatgg gaatttttca taaagataga gaaaatcaag ttatagagaa
     3841 aaatctaagt cgagtaaaaa ataaagaact tttaccttat gcagaggaga tgcttcatgc
     3901 attaatggat ataagtaaaa aatatcaaca atataaaata gagatgagag aaaattttat
     3961 taagtaaaat gaaataaaat tgtattttaa gtgggaaaaa ggattactta gagaagaagc
     4021 cttaattgaa taatagagca taatttaatt agaattaaat ggaaaaatat attttggtgg
     4081 ttttaagaga ttaaataggt gattatagga ttttagtatg ttataaaaaa agcggaattt
     4141 taaaatgtta ataataatgg gtgtcaaatt aacaagacat ccatttattt taataaagca
     4201 taaacttttt ttgaaatacc ctatagaaaa tggataagag agtagaataa tgattgtcaa
     4261 caaataatta cttatagaaa aagagggtat caaatttgga aattaaaact ttgattaaat
     4321 ttttaaaatt ggtaatggta ataactctta tatatacgtt aattactact aattttgcca
     4381 taagagcatt tttaattgtt gtagaagtga tttttattat tatattttat aaggttaaaa
     4441 atatataaat aagattctaa gtaaatatta tatgattaaa aaatatattt tttaaatact
     4501 attctaaagt gaaaagacac ttagtatggc aactaaatgt cttacatgaa cttgtgtatt
     4561 tggttttgga tatttaaaca aaaacttaaa gttattatta atctttaaac aggggatgtg
     4621 aaaagattat tataagtttt gtatggtgat ttaatcatag aattatgaat taattatgga
     4681 attttattat taaagtttaa atcttaatta ttgtatagta taagagtttt atctttattc
     4741 aatgttttta ttagaattaa attgtaaaga ttaaggaggt ggagttttga agttatttgg
     4801 attaataggc gagaagttag gacatagttt atctccagag atacataata aagtttttaa
     4861 agataataat atagatggat tatataatct tttttctgta aaaaaagatt ttgaaaataa
     4921 tattgtagaa agtctaaaat gcttaggagt aaggggagcc aatgttacaa taccatataa
     4981 agagaaggtt atggatcagc tagacattat ttcacatgag gccaaagcta taggagcagt
     5041 aaatactatt ttaataaaag atggaaaatc ctatggttat aacactgatt attatggatt
     5101 tggtaaaatg ctagaaagag caaaggttga tattgaagga aatagctttt ttgttttagg
     5161 agcaggggga gctgctagaa gtattttaaa atatttagag gatagcaaag ctaaaaaaat
     5221 agttttggtt tctagggaca aagagaaagt ttttaaaaaa tttaaagatt tcaatattaa
     5281 ttttatgagt tatggtgagc ttgaagaaat tcatgaagag tttgctttaa ttaatacaac
     5341 tccctgtgga atgtatccta atacaaattc agtagctgtt tcagaaaagg taattaaaaa
     5401 gtttaaggtt gctttagata tagtctataa tcctttagaa actaagtttt taaaaatggc
     5461 taaggataat ggtcttaaaa cggtagatgg attatttatg ttagttggac aaggggttaa
     5521 agctgaagag atttggaatg gaataaaagt agataagtct acagaagaaa atatatatga
     5581 agaattgaaa tgtagatttt aaagttaatt ttgtaataag aaaatgttaa ttagaatact
     5641 aagtttagcc aaatatgata taaaataagt atagattaca tttttaaaat aggaataaaa
     5701 actaaaatta atttcaaaag tttaaataat attgttgggt aaaatacaaa aaggggatta
     5761 ggatatgaaa aaaagaggag tacttttaat tggaatgcct ggagcaggaa agacaaccat
     5821 aggaagagag ctttcaacgg tattaaagat gaatttttta gacatggatg aattcataga
     5881 aagaagtaca ggaaaagaaa taaaagaact ttttgctcag ggagaagagg tttttagaga
     5941 tttagaaagt aaaagttgtg agcttttatc aaccttaaaa aatgtggtta tatcttctgg
     6001 tggcggaata gttacaagag aagaaaatat gggtaatttt aaggagttta taactgtttt
     6061 tataaatagg cctttagatt taattatgga agatatagac acagaaggaa gacctctttt
     6121 atgggatgga aaagaaagac ttactaactt atatagagat agaattgatt tatataaaaa
     6181 gcatagtgat atagaaatta taaatgatgg tactactaaa gaggctatta gggaaatagt
     6241 cgaagctatt tcagaattag cctattaaaa atcctaaatg gaaagtaggt ggattttaac
     6301 caaataaaaa gttaataatt tggttagaga tattatgaaa ataatggtta taaatggccc
     6361 taatttaaat cttctgggga ttagagaaaa agaaatctat ggtgctaaag attttaatca
     6421 ggtcattgat tatataaaag aagaaggaaa agagttaggg ttagaggtaa attgctttca
     6481 gagcaatata gagggagaaa taattaattt tattcataat gcatactttg aaaagtatga
     6541 tgggataatt ataaatcctg gagcatacac acattatagc atagctattt atgatgcttt
     6601 aaaaggggtt gaaataccaa ctgttgaggt acatttaagt aatattcaca aaagagaaga
     6661 gtttagacat aaatcagtta cagccccagc ttgtattggg cagataagcg gatttggtga
     6721 atatgggtat attatggcaa tgaatgcatt aaaaaataag attaaataaa aagaaactcc
     6781 ttcaaagttt tactttgaag gagtttctaa gtttccaaag tattttctag accaatcttc
     6841 caaacattct aagacgggtg caagctcttt tcctttttca cttagataat attcaactct
     6901 tggaggattc tcatcgtaaa ctatcttttt aacaagttca tcttcctcta attccttaag
     6961 tttttcagtt aatactttat gacttattcc acatgtaatt ctctgaagtt caagaaacct
     7021 ttttttccct ttatttaagt gacacaaaat aactgccttc cattttccgc gtataagttc
     7081 cattgctaaa tctaataggc aaacatagtt tttatcttta tatttcaaca aagtttatca
     7141 tctccaaaat ttgtttctat tttaaaatcc atgataacat tatatattaa tgatttttat
     7201 aactcaaata attataagag gtcacttact aaaaagtgcg tacttacata aataatatta
     7261 attttatata ctatgttcat ataatgagaa aaaatttata agcttataaa ctattattcc
     7321 aataaaagaa aaactgagtt tattaatata aaaaataaaa tttagtatta tttttaggag
     7381 gaaaatatga aaacattatt tgataaaact aacttaggaa caattaacat gaaaaataga
     7441 tttttaagag gagcattatg gacagcgtta gctgatgaga aaggtcatat gacaaatgaa
     7501 ttatcagacg tatatgaaga attggctaga ggtggagtag ggacaatagt aacaggatat
     7561 gcttttgtaa ccgagaatga acagccaaat ccaagaatga tgggaatata tgatgattct
     7621 tttataccag aatataagga atttacagat aaaattcata gacttggatc taatataata
     7681 atgcaaatag tatacggtgg atttatgact gaatttaatg taggagaaag agttatatgg
     7741 ggaccttcta ctactaaaaa tgaagttaca ggaactttat caaaagaaat gactaaggaa
     7801 gatataaagt atttagttaa tgcatatgca gaagcagctt taagagttaa aaaatcagga
     7861 tttgatggag ttgagataca tggaggacat ggatatttag taagtcaatt cttatctcct
     7921 tattataata aaagaacaga tgagtatgga ggaagtatag aaaatagggg aagatttgtt
     7981 tttgaaatat ttaaagcaat aagagaaaaa gttggagaaa aattcccaat attaataaaa
     8041 ttaaattctt cagactatgt aaaagaaggt ggattaacta tagaggatag tctatatgta
     8101 gctgaaagat tagctgaatt aggaattgat gctattgagg ttactggagg aaatgaatct
     8161 atagaagaag ttatgaaaaa taacttagga ccatcaagaa ctaaagttgc tatttcaaag
     8221 gatagagaat cttattttaa agaatatgct ataaagctag ctgaaagaat aaacaaacct
     8281 gtaattttat caggtggtaa tagacacttt gatgttatgg aagagttatt aaatgataca
     8341 aatatagaat atttttcact ttcaagacct ttaacagcag agccagactt aataaataaa
     8401 tggtattcag gagatttaaa gaaacctaag tgtaagtcat gtaatcaatg ctataaaact
     8461 tatggaaaga gatgtatttt caatattaaa taaattaatt aaaaaataaa gaagtattac
     8521 ttattaaaag gtacaatcct tattatagga tgtacctttt tttaggtaaa aaagtttagt
     8581 tagaatatga attttgaggt taattatagt actaaaaact tagtgatagc tagatttaga
     8641 ataagtttac agtaagttaa aataattagg aggaatattt aaggtgaaag aggtatcatt
     8701 atattataaa gttgtggatt ttaatatagc ttatatgaga aaatttaggt tttgcatgct
     8761 gttaggtata aaataactaa ttaggtttta caagtattta tatactctaa gtaactatat
     8821 aaaatccata aattttataa acttttattt aaatagcatt ttaaataata atattattat
     8881 tacttagttt tatgtattag caagatatat ataaggataa caatagttgg actttgattg
     8941 gaggttcata tgctaaggcg tcttatttaa atctgaggaa tagaaaatag tgagaaatat
     9001 tgacttactt gttatttgta tttatttaag aagatattat aaaatttata ggtatttata
     9061 aaatgaattt aaaaagggtt tagttaatag ttaaaattta gctgtgtata tagtaaagtt
     9121 tgtaggggat agatatagga agttggttaa aatagcaaat gagaatagag aagttagaat
     9181 agtttaaagt tgaatataaa ttttattata aggataaaag gggattttta tgaaggagag
     9241 aattgagaat ttacagagaa taataaaaaa tattttgtca tctacagatg atcaaattca
     9301 gggtgattta agagattcct taagattaag aatgtctgta tcagaaaatt tacatgggga
     9361 agtaagatat ttaaaatgtt taagagcttt agtagaagaa aaatttcaag aattttttaa
     9421 gaaaaaaaac tttccaaaag attataatga ttttgtagga atatggtcaa gcttatcaga
     9481 agaagcgaag tctttatgta atgatccaaa atatgattat gatgaagaaa aatataaata
     9541 tgaatttata tattttgagg tttattgtaa tgtttattta agatattctt taggactact
     9601 ttttaatggt aataataaag atacaattga tgacttaagc caatataata gtttagaaat
     9661 aattcaaatg tatagatatt atcttcatca aataactctt aaaaagttag agaaaagttt
     9721 aaagtcagat aaagttaata gttaatataa aaagaagttg tattcttatt tggaatacag
     9781 ctttttttat tatgtttttg taaattatac catagagtat tctatactat agagtacttt
     9841 atggtataat tatacttggg agggataata ttggaactta ataaagagat tataaaagga
     9901 catgtggaaa gtataatatt atcactttta caaggaaaag atttatatgg gtatgagatt
     9961 tcaaaattaa ttagagaaaa cagtaaagaa gcctttgagg tcaaggatgg aacattatat
    10021 gttgttttaa aaaggttaga aaaattagga cttgtaatat catactgggg tgattgtaaa
    10081 agtggtggtg gaagaagaaa atattttaaa attacagagg atggagttaa ttatctaaaa
    10141 gaaaaaaaga gtgaatggga atttctaaag agtattatga atattttttt ggggggagaa
    10201 taaaatgaga gagatagata attatattga ctctttatat aagaatattg atggaatttc
    10261 aaaagaaaat aaagagataa aggaaactat gaggactcat ttaatagaat ctgtagaaga
    10321 attaaaatta gaaggattta gtgaaaaaga gagtattaag attgctttag aacgatttgg
    10381 agaaataaat gaaattagag gagaactaaa aactgtagtg gaaagagggg taaaaccact
    10441 actatataca gtgggaattt cattaataat acttatagct ttctttaccc atttaatatt
    10501 tatgagattt gattatataa aattgctagc tttaataatt attgctgttc caatgtatat
    10561 aataattaga ggtggagttt taatatatta taaagtaaag ggtataaaat atgatataga
    10621 ttggaaaaag gaattatata aatttttatt tgctatttat tttatatttc tagttgggtg
    10681 gtatatattc cctatagaag ttggaggaag ctcaaatgtt aattttcaag tagacttaat
    10741 tccttttaaa agttatattg aattaatgaa taggggaata actataggat atcttatgaa
    10801 atggataatt attagattga ttttatttat acctttagga ttttatccag catttctaaa
    10861 aggaaaattt aataatatag ctggttgtgt actaatagct tctatagttt attttatagt
    10921 tcctatatta gaattaatat taagttttgg attaatttct gtaccaataa tttatattgg
    10981 aatagattat tgggttataa gtataattgg agttttactt ggttattttt tatataattc
    11041 aacagctaaa aattataaat aaaaaaaggt cgattatgac cttttttatt tttata
//
LOCUS       DAEVGI010000080        10926 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEVGI010000080 DAEVGI010000000
VERSION     DAEVGI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.080"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(102..1313)
                     /locus_tag="K8M77_001660"
     CDS             complement(102..1313)
                     /locus_tag="K8M77_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI7086154.1"
                     /translation="MSKKKVIVVGGGPAGIMASLAASANNEVVLIERNNEIGKKLRLT
                     GGGRCNITNNREIEEFFDKIVTNNKFMYSALYTFTNYQLLDFLSKKGLEYKVELDQKV
                     YTKSDTAEEVISIFKNELNDCRVKVLYNTKVCDFIVEEDEIKGIITEQGKKILGDKII
                     ITTGGKSFPNTGSDGEMYGILEKYGHTITSIHAALIPLVTKEDFVKNLQGVAMKDVVV
                     SAKIKKKKYEKFGDMIFTHFGVSGPAILKLSSYITKALDQGEVELNLDFLSDKSKEEL
                     SQIIRSNPNKTVLNNLKGTLPQNFLKQIFEMLGLTEIRASDLKKEDENKIISYIKEMK
                     LTVRETLTIKAAQVTSGGVKVKEINASTMESKIIKNLYFAGEVIDIDAETGGYNLQMA
                     FSTGYLAGSDF"
     gene            complement(1488..2819)
                     /locus_tag="K8M77_001661"
     CDS             complement(1488..2819)
                     /locus_tag="K8M77_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HBI7086155.1"
                     /translation="MNPKEILDTLDYLNINEIEMANVELGEMGKERIRKKIKNTIKNS
                     RNKKIKKTAIAVTLAFGILTIGVISKPVIAANIPIIGDIYKELGFFKGFEEYTNYIGE
                     TKEVDGYKFTIDNIVGTPTDLLIGVKLESPVPLKEKVQEDNFRIDVDMSNFGINSWSS
                     MTDINYLDEYTAVLTAEISTTENKFKELGEINLSATKVLDDETYISCNFDVKVDFNSS
                     FRNIEKLNVNEIINEKIKIKFIEGNIMESKIWFNDLGAFEENAVFYLMVDGKAYMWWD
                     GNGVEDTSFIIPRMVSVLNFPNVKYEDIKNAKDIKIIYGEIGEGYRENLDIKLTEREG
                     IKYPGEINYEEDLKGGFYDVKRENGMIKFYFKSNYNPLTLFRDIKLREVKGEEKYGQS
                     ILGGFYENPEKEGEYVLEFEDIDTNNSLELVYSHRTNNLSELKDYKVIDIK"
     gene            complement(2821..3363)
                     /locus_tag="K8M77_001662"
     CDS             complement(2821..3363)
                     /locus_tag="K8M77_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBI7086156.1"
                     /translation="MINKGNFLKQLKKKNEKALDYVIDSYSDVIFKVAYGVLNNRQLS
                     EECVNDVLLKIWDHIHHFNKDEDKFYPWILAITKYTAIDILRKELRHSNTLEISSLNI
                     SEEKCLVSKFHNKEQLNMVTEEINAMKDTDREIFLRKFYLKETGEEISKKMGLSNKFI
                     NLRIFRGRNKLKAKFMGRYV"
     gene            3761..3988
                     /locus_tag="K8M77_001663"
     CDS             3761..3988
                     /locus_tag="K8M77_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI7086157.1"
                     /translation="MNSILPQAVNLLSYINSGETFIIKDLFPKIIWDKLSYTEKRTLE
                     SAFLKHINKKVDLRIKALNEIREGLKVYEKL"
     gene            complement(4093..4998)
                     /locus_tag="K8M77_001664"
     CDS             complement(4093..4998)
                     /locus_tag="K8M77_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7086158.1"
                     /translation="MEGFIIGLSLTILIVLLIIFILKTKEINRLTLELKKLNSEGKIE
                     KLRLSLPNKNIENLIIEINTLIDDKRKMENIYKEKDMELREAIANMSHDLRTPLTSIM
                     GYVYLLNDDKLDKEERKEYLKIIEKRSLVLNDLITNFYGLSRIQADQYEIKFKPVNLE
                     LVLGEIIAAFYETLDYKFGEPEINIEEGLGPVLGDKQALNRIFTNLIENIIKHGEGEV
                     KISLKKKNKYIVMEFSNKAEDLESKDVNRIFEKFFTKDRMRTGQNTGLGLAIVKLLVE
                     KQGQKIEAKKVGNRLVINIIWSLES"
     gene            complement(5012..5764)
                     /locus_tag="K8M77_001665"
     CDS             complement(5012..5764)
                     /locus_tag="K8M77_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086159.1"
                     /translation="MLNLIKAELYKIARRPLYITICIIFNLLIILSGIGMHYAFLESS
                     RIPDFTALVFITINFMGAIPWIMLMFIDVVLEEYKENTLKNLISSSISREKVYLSKYI
                     VQVIIFLVIEITAVLTMIGTSYALGLVGPNGFSDFMEVILRLLIAFPIYISALAIYDF
                     ITIITKKEGLAVVVILGIVSGVPMIFSILSSMYEGIFILIYPYLLFNPLKSLVSEVLT
                     TKIIGTNIIVGIINTVVFLILGILVIRKQEVK"
     gene            complement(5757..6677)
                     /locus_tag="K8M77_001666"
     CDS             complement(5757..6677)
                     /locus_tag="K8M77_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086160.1"
                     /translation="MGNIISIRNLNKNYGKKNILDNLSMNLKKGDIYGLVGKNGAGKT
                     TLIRIILSLANYDSGEIELFGEIDEKKKLHEHKKIGNIIETPSFYSFLTAKQNLEYYR
                     IQRGIVGEKSIDEILEMVGLGDVGNKKFKKFSLGMKQRLGLALALLGEPELLILDEPT
                     NGMDPMGIKEVREVLINLNKKNGVTILVSSHILGELSQLANCYGFLKDGKIIEEISSE
                     ELNEKCRNHLLIKVDDCKKAVVILEKVLDNNSYEVLPDRFIKIYNKLDEPQLINRALV
                     ENGVEVYSLERIGVNLEDYFVDLIGGEYNA"
     gene            complement(6887..7576)
                     /locus_tag="K8M77_001667"
     CDS             complement(6887..7576)
                     /locus_tag="K8M77_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7086161.1"
                     /translation="MINSESILVVEDDIDINNLLSKILEKEGFKVRQAFSGSEGKMCL
                     EFFDFDMVLLDLMLPGITGEIFIDEIRKIKNTPIIVISAKTTMEDKINVLKLGADDFI
                     SKPFDVNEVLARVEALLRRSKITNSSEGVETIKYKNLILEPRSREVKVKGEEIQLTSK
                     EFKILELLVCNPKKVFTKANLFETVWEEEFLGDDNTINVHISNLRNKINLLDNENDYI
                     KTIWGVGFKMD"
     gene            complement(7921..9228)
                     /locus_tag="K8M77_001668"
     CDS             complement(7921..9228)
                     /locus_tag="K8M77_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBI7086162.1"
                     /translation="MINIKLILAIVLSILATYAIYKIRKITNKFSFATLTALTLGVVL
                     GIIFKENILFLDTVGKAYMSLIKMIVVPLVVTSLITSIVRLENLDTLKSIGLKTFTVL
                     LGTTGAAALIGIIVASSLNLGQGLRFIGAENFKAREIPGFSKVLIDMLPSNPLAAIVE
                     NKIIPIVIFSMFIAIALVIEDNTNKEKAKPFKDFILSAYDIVLRITKMVLRIIPYGVF
                     ALIATAAAKNGMDTLMSLIWVILAVYIAAFLQFLFVYTPLISFVARMNPLKFFKGIFP
                     AQVVAFTSQSSYGTLPVTIKSLVEGVGVSENIASFVAPLGSTIGLNGCGGFYPAIVAI
                     FAANVFNVELTSYSYILIVLTAIISSIGIAGVPGSATMSTTVMLAALGLPIEALAMVI
                     AVDSIIDMIRTATNVTGASVAALIVDQTEKRKEYKVEESVQRA"
     misc_binding    complement(9369..9605)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9986..10867
                     /locus_tag="K8M77_001669"
     CDS             9986..10867
                     /locus_tag="K8M77_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086163.1"
                     /translation="MEKDLFNNEALDDIIFRLSKNTLSDFFYEKIGERNMIISGLNSD
                     FSGVASTTLTGCSFQLQQLTLFKRLLNEGELILEFLLENKDEFRNWFNNNPYAYFKHC
                     SIFKNSTLEKDYYKDFKSSYNELTSKYNLNLLNFISSLIFLSSYNCCIKKKANYEEAF
                     VKAILENHIDKVYEKSIDIKNFLEKLELKHLEFKDEIRNLLVLISSESLYFEDIPLRL
                     LDYANATDILTVIILIVNVKEYISESKVIPLNLSKNSFMSTEEYKKFMNSISESYIRI
                     YDFDEEVRKKIEEDLKL"
BASE COUNT         3749 a         1828 c          993 g         4356 t
ORIGIN      
        1 acctttaaaa tataaaagac ttacaaagag attttgagaa ttagtagatg aatctccata
       61 tcctttgtaa atttttactt tgataatttt aattaaagaa tttaaaaatc acttcctgct
      121 aagtatcctg ttgaaaatgc catttgaaga ttatatccac cagtttctgc atctatatct
      181 ataacttctc ctgcaaaata aagatttttt ataatctttg attccatagt agaggcattt
      241 atttctttaa ccttaacacc accagaagtt acctgagctg cctttatagt taaagtttct
      301 cttacagtaa gtttcatctc tttaatatat gaaattattt tattttcatc ttcttttttc
      361 aaatcactag ccctaatctc tgttaatcct agcatttcaa atatctgttt taaaaagttt
      421 tgaggtaatg ttccttttaa attatttaat acagttttat taggattgct tcttattatc
      481 tgtgataatt cctccttgga tttatcactt aaaaagtcta agtttaactc aacctctcct
      541 tgatctaaag ccttagttat ataagaagat aattttagta ttgcaggacc agatactcca
      601 aagtgagtaa atatcatatc tccaaatttc tcatactttt tcttttttat tttagctgaa
      661 acaacaacat ctttcatagc tactccttga agatttttaa cgaagtcttc tttagtaaca
      721 agaggaatta aggctgcatg aatagaagtt attgtatgac catatttttc taatatacca
      781 tacatttctc catctgaccc tgtattagga aaactctttc cacctgtagt tattattatt
      841 ttatcaccta aaatcttttt accttgctct gtgattattc cttttatttc atcttcctca
      901 actataaaat cacatacctt agtattatat aaaactttta ctctgcaatc atttaattca
      961 tttttaaata tgcttattac ttcttctgct gtatcgcttt tagtatatac tttttgatct
     1021 aattcaacct tatactctaa gcccttttta cttaaaaagt ctaaaagttg atagtttgta
     1081 aatgtatata atgcactata cataaacttg ttatttgtaa ctatcttatc aaaaaactct
     1141 tctatttctc tattgttagt tatattacat cttccgccac cagtaagtct aagcttttta
     1201 ccgatctcat tatttctctc tataagcaca acctcattat tagctgatgc tgctaaagat
     1261 gccattattc ctgctgggcc tcccccaact actattacct ttttcttact catttgtttc
     1321 ctccaaattt tcattttgaa aatatctcca aaaatttata tcttattctt tatgattaac
     1381 tgaaattaaa ttctactttt aataatttaa ttttaaaaaa tatataagta ttatacacta
     1441 aaaaaggtga gcaaaactac ataataattt aattcacctt aatattttta cttaatatca
     1501 attactttat aatcttttag ttcacttaaa ttattagttc tatgagaata aacaagttct
     1561 aaagaattat ttgtatcaat atcttcaaac tctaatacgt actctccttc tttttctgga
     1621 ttttcataaa atcctcctaa tatactttgc ccatattttt cttcaccctt tacttctctt
     1681 agctttatat ctctaaataa agttaaagga ttataattgc tcttaaaata gaattttatc
     1741 atgccatttt ctctttttac atcataaaat ccacctttta aatcctcttc ataattaatt
     1801 tctcctggat actttattcc ttctctttct gtaagtttaa tatccaagtt ttctctataa
     1861 ccttctccta tttctccata aataatcttt atatccttgg catttttaat atcttcatat
     1921 ttaacatttg ggaaattaag tacagatacc attctaggaa taataaaaga tgtatcctct
     1981 acaccatttc catcccacca catataagct tttccatcaa ccattaaata aaaaacagca
     2041 ttttcttcaa aagctccaag gtcattaaac caaattttag attccataat gtttccttca
     2101 ataaatttaa tctttatttt ttcatttatt atttcattaa catttaattt ctcaatgttt
     2161 ctaaaacttg aattaaagtc taccttaaca tcaaaattac aacttatata agtctcatca
     2221 tctaaaacct ttgtagcact taaatttatc tccccaagct ctttaaactt attttctgtg
     2281 gtacttattt ctgccgtaag aactgctgta tattcatcta aataatttat atccgtcatt
     2341 gaactccaag aatttattcc gaaattactc atatcaacat caattctaaa gttatcttct
     2401 tgaacctttt cttttaatgg cacaggactc tcaagcttaa ctcctattaa taaatctgtt
     2461 ggtgttccta cgatattatc tatagtaaat ttatatccat caacttcttt tgtctctcct
     2521 atataatttg tgtattcctc aaatcctttg aaaaatccaa gttctttata tatatcccct
     2581 attattggta tattagctgc tattacaggc ttactaataa ctcctattgt taatattcca
     2641 aaagctaatg taaccgcaat ggcagttttc ttaatttttt tatttctact attttttata
     2701 gtatttttaa tcttctttct tattctctct tttcccattt cccctaattc tacattggcc
     2761 atctctattt catttatatt taagtaatct aaagtatcta aaatttcttt tggattcatt
     2821 ttatacatac ctccccataa atttagcctt taatttattt cttcctctaa atatcctaag
     2881 gtttataaat ttattactta accccatttt tttacttatt tcttcccctg tctctttcaa
     2941 ataaaatttt cttaaaaata tttccctatc agtatctttc atagcattta tttcttcagt
     3001 aaccatattt aattgctctt tattatgaaa cttactaact aaacactttt cttctgatat
     3061 attaagactt gatatctcta aggtattaga atgtctcaat tctttcctta atatatctat
     3121 agctgtatat tttgtaattg ccaaaatcca aggataaaat ttatcctcat ctttattaaa
     3181 atgatgtatg tggtcccata tttttaaaag aacatcatta acacattcct cacttaactg
     3241 tctattattt aaaactccat aagcaacttt aaaaatcaca tcagaataac tatctataac
     3301 ataatctaaa gctttctcat ttttcttttt caattgctta agaaagttcc ccttgtttat
     3361 catttaattc cctcctaaac ctgaaattac tttcttattc aataatatat tcgtaagtta
     3421 tttaataact atttcacttt tatcaaaaaa tttataaaaa aacttaattc tcttttagta
     3481 aattgaaatt tttactttga aaccaatatt gatttttata ttaaaaatat acaaaattat
     3541 catctctttc actcgtaata tttacttgct aaataaattg atcattaaaa tcattaaata
     3601 ttataatact atgcatctta aaactttttt gtagaaattt ggcacaaaat ttaggtttca
     3661 tcatattatt aattcaaatt tattcctaaa gttcttaata aaaaatttta agcatatatt
     3721 atacttagta gtttaattta cgttttaagg aggttttctt atgaattcaa tattaccaca
     3781 agcagttaat ctattaagtt atataaattc tggagaaaca ttcataataa aagatctttt
     3841 tcctaaaata atttgggata agctatcata tacagaaaag agaaccttag aatccgcatt
     3901 tttaaagcat ataaataaaa aagttgatct aagaattaaa gcacttaatg aaataagaga
     3961 aggactaaaa gtttacgaaa aactttagaa ttatatttat taaaataaaa aaataagcaa
     4021 tttaatttca aattgcttat tttatacttc taaaaaatta atttttctaa attccccaaa
     4081 gtctaaatcc ttttaacttt ctaagctcca tattatattt ataacaagcc tatttccaac
     4141 ctttttagcc tctatctttt gtccttgctt ttctactaat agtttaacta tggctagccc
     4201 taagccagta ttttgtcctg ttctcattct atccttagta aagaactttt caaagattct
     4261 atttacatcc ttagattcta agtcttctgc tttattagaa aattccatta ctatatattt
     4321 atttttcttc tttaaggata tttttacttc tccttcacca tgctttatga tgttttcaat
     4381 taaattagta aatattctat ttaaagcctg tttatcacct aatacaggtc ctaagccctc
     4441 ttcaatatta atttctggtt ctccaaactt ataatctaag gtttcataaa aagccgcaat
     4501 aatttcaccc aagactaatt ctaaattaac aggtttaaat ttaatttcat actgatctgc
     4561 ttgtattcta gacagtccat aaaaattagt tattaaatca tttaaaacta aagatctttt
     4621 ttctattatt tttaaatatt cttttctttc ctctttatct aatttatcat catttaataa
     4681 ataaacatat cccattatag aagttaaagg agttcttaaa tcatgagaca tatttgcaat
     4741 ggcttctctt aactccatat ccttttcctt ataaatattc tccatttttc ttttatcatc
     4801 tattaatgtg tttatctcta ttattaaatt ctcaatattt ttatttggaa gtgataatct
     4861 taatttttct attttcccct cactatttaa cttttttaac tccaaggtta atctatttat
     4921 ttctttagtt tttaaaataa atattattaa aagcacaata aggattgtca aagacaatcc
     4981 tattataaat ccttccaata tacatcctcc tttatttaac ttcttgtttt cttattacta
     5041 aaattcctaa aattaaaaat actactgtat ttattattcc tactataata ttagttccta
     5101 ttatttttgt agttaaaact tcactgacta aactctttaa aggattaaat aataaataag
     5161 gataaattaa aatgaaaatt ccttcataca tacttgataa tatagaaaat atcattggta
     5221 ctccagaaac aatacctaag ataactacaa ctgctaaccc ttccttttta gttattatgg
     5281 taataaaatc atatatagct aatgcactaa tatatattgg gaaagctatt agtaatctta
     5341 atattacttc cataaaatct gaaaatccat taggaccaac taatcctaag gcataagaag
     5401 ttcctatcat agttaatact gctgttattt caataactaa aaatataatt acctgaacta
     5461 tatatttact taaatacact ttttcccttg atatacttga agaaattaaa tttttaagag
     5521 tattttcttt atattcttct aaaactacat ctataaacat aagcattatc catggaatag
     5581 ctcccataaa attaattgtt ataaaaacta atgctgtgaa gtcaggtatt ctagaacttt
     5641 ctaagaaagc ataatgcatg cctattccag ataaaattat aagtaaatta aatattatac
     5701 aaattgtaat atataaaggt cttcttgcta tcttataaag ttctgctttt attaaattaa
     5761 gcattatatt cacctccaat taaatctaca aagtaatctt ctaagttaac tcctattctt
     5821 tctaaagaat aaacttcaac tccattttct actaacgctc tgtttattaa ttgaggttca
     5881 tctaacttat tataaatctt aataaatcta tcaggtaaaa cttcatatga gttattatct
     5941 aaaacctttt ctaaaattac taccgccttt ttacaatcgt caaccttaat aagcaaatga
     6001 ttcctacatt tctcattaag ttcctctgat gaaatctctt ctattatttt accatccttt
     6061 aaaaatccat aacaatttgc aagttgtgat aactctccta aaatatgact tgatactaat
     6121 attgtaactc catttttctt atttaaatta attaatactt ctcttacttc tttaattccc
     6181 ataggatcca ttccattagt aggctcatct aaaataagta attctggttc tcctaaaagt
     6241 gccaaggcta aacctaatct ttgcttcata cctaatgaaa actttttgaa ctttttattt
     6301 cccacatctc caagtccaac catttctagg atttcatcaa tagatttttc tcctactatt
     6361 cctctttgaa ttctataata ttcaagattt tgctttgctg taagaaaaga ataaaatgat
     6421 ggagtttcta ttatattacc tattttctta tgctcatgaa gctttttctt ttcatcaatt
     6481 tcaccaaaaa gttctatttc tcctgagtca tagttagcta aggataaaat aattcttatt
     6541 aaagttgttt taccagcacc atttttccca actaaaccat atatatcgcc tttttttaga
     6601 ttcatactta aattatcaag aatatttttc ttaccataat ttttgtttaa atttcttata
     6661 gaaattatat ttcccaaaat ctttacctcc taataagttt tctctttatc tttctctatg
     6721 tttatataat aaaccttaag tctcaaaata ccttaaagaa aaccttaaga aagtcttaat
     6781 ttttctatta actcttaatt aaatgtatat ttaaaatata aactcctcta tattttgata
     6841 tttattcaaa tcaacttatt aagaaaatta tgaatcatca atgtagctaa tccattttaa
     6901 atcctactcc ccaaatagtt tttatataat cattttcatt atctaaaaga tttatcttat
     6961 ttctaaggtt gcttatgtga acatttatag tattatcatc acctaaaaac tcctcttccc
     7021 atactgtttc aaataagttt gccttagtaa aaactttctt tggattacaa actaaaagtt
     7081 ctaatatttt aaactctttg gaagtaagtt gtatttcctc tcccttaacc ttaacttctc
     7141 tacttctagg ttctaatata aggttcttat attttattgt ctctacacct tcacttgaat
     7201 ttgttatttt acttcttctt aaaagcgctt caaccctagc taaaacttca ttaacatcaa
     7261 aaggtttact tataaaatca tcagcaccta attttaaaac atttatctta tcctccattg
     7321 tagtttttgc tgatataact attattggag tgttctttat ctttctaatc tcatctataa
     7381 atatttcacc agttatacct ggtagcataa gatctaaaag taccatatca aaatcaaaaa
     7441 attctaaaca catcttacct tcagatcctg aaaatgcttg tctaacttta aacccttcct
     7501 tctctaaaat tttactcaat aaattattta tatctatatc atcttcaact actaaaatac
     7561 tttctgaatt aatcataatt ataacacctc taaaaattaa tttataattg tatttttaaa
     7621 actttctata ccaaaataat accataatac tacagttaga tttatttaaa ataatcttaa
     7681 attttcattt aacccttaat ataaaataaa atttatatat taatatcaaa ttaaagattg
     7741 gaaagaatgt attctattta aaaaataata atttttctat atacttatat caaataaagc
     7801 ttaaaggagc tgaacaaaac tttagtttaa aacttattaa taaacaagct ttattaagtc
     7861 tttaattcaa ctccttatat tagatattta tgtattgtat tttaaaataa atttttaatt
     7921 ttatgctctt tgtactgatt cttcaacttt atattctttt cttttttctg tttgatcaac
     7981 tattaatgca gcaactgaag cccctgttac atttgtggca gttcttatca tatcaattat
     8041 agaatccact gcaatcacca ttgctaatgc ttctattggt aatcctaaag ccgctaacat
     8101 tacagttgtt gacattgttg ctgatccagg tacacctgct attcctatag aagatattat
     8161 agcagttaaa actataagta tgtatgaata actagtaagt tctacattaa aaacattagc
     8221 tgcaaatatt gcaactattg ctggataaaa acctccacat ccatttaatc caattgttga
     8281 tccaagtggt gctacaaagc ttgctatatt ttctgatact ccaacacctt ctactaaaga
     8341 ttttattgta acaggtaaag taccataact actttggctt gtaaaagcta caacctgcgc
     8401 tggaaaaatt cctttaaaga attttaatgg attcattctt gcaacaaagc ttattaatgg
     8461 agtatataca aataaaaatt gaagaaaggc agctatataa acagctagta taacccatat
     8521 taatgacatc aaagtatcca ttccattttt agctgccgct gtagctatta aggcaaatac
     8581 tccatatggt attattctta ataccatttt agttattctt aaaactatat cataagctga
     8641 taaaataaaa tctttaaatg gctttgcttt ttctttatta gtattatctt caataactaa
     8701 ggcaattgct ataaacattg aaaaaataac tattggtatt attttatttt ctacaatagc
     8761 tgctaaagga tttgatggta gcatatctat aagtacctta gaaaaccctg gtatttctct
     8821 tgccttaaaa ttttcagccc ctataaatct taaaccttgt ccaagattta aagaactagc
     8881 tacgataatt cctataaggg ctgccgctcc tgtagttcct aataaaacag taaatgtttt
     8941 taatcctatt gattttaatg tatctaaatt ttctaatcta acaatactag ttattaagga
     9001 tgttactact aaaggaacta ctatcatctt aattaaagac atataagcct ttcctactgt
     9061 atctaaaaat agtatattct ccttaaatat aattccaagt actaccccta aagttaatgc
     9121 tgtcagcgta gcaaatgaaa atttatttgt aatttttctt attttatata tagcatatgt
     9181 tgctaatatt gaaagcacaa ttgctaatat taatttaata ttaatcataa aaaatttcct
     9241 cctataaaat tacttttaat ttaatattgc taagctttta tatcaaaagc caagcttaca
     9301 aagtataatt ttatttttat atgctggcca gcaaaatata ataaaaaaag ccctctgcct
     9361 ctgtaaatta cagaggcaga agactatact tccacggttc cactctgatt agatatataa
     9421 tatccatctc tatttaggta cattcatacc ctaactctat aatgggagtt cccataaaca
     9481 cctacttaat tcggcactta agctccaaga ggcacttcaa taatactcat tacaggttat
     9541 ctcagcaaac taacctctct ctgtagtaac ttataatatt tactttccct cttcaacgca
     9601 tttaaataaa tatttttctt gttttaaata atatattatt tattttttaa agtcaacata
     9661 ttttttcaac ttaaattatt tcttgacaat ttcatatttt aaatggtatc attattaagc
     9721 agttggtacg tatagtgcta attcttttgt agagcttggt ttagtccttc gccggatgcc
     9781 aagctctact tttttatccc aaatttaatt taaagatttt aattgtgaat aattattata
     9841 aatattaatt cttaaagaaa gttcctatat aaacaaatta gaattcctat gcatatacta
     9901 tagcgatgtc tacattacta tagaagggag gcctactcaa taggtgaata ttatctaatg
     9961 attttatact cttattgaag attttatgga aaaagatctg tttaataatg aagccttaga
    10021 tgatattatt tttaggttat caaaaaatac tctttcagat tttttctatg aaaaaattgg
    10081 tgaacgaaac atgataatta gcgggctaaa ttctgatttt tctggagtag cttctacaac
    10141 cttgactggc tgctcttttc aacttcaaca attaactctt tttaaaagat tactaaatga
    10201 aggagaatta attttagaat ttttgcttga aaataaagat gaatttagaa attggtttaa
    10261 taacaatcct tatgcttatt ttaaacactg ttctatattc aaaaactcaa cattagaaaa
    10321 agattactat aaagatttta aatcttcata caatgagcta acctctaagt ataatttaaa
    10381 cttgcttaat tttatatcat ctcttatttt tctttcttct tataattgtt gcataaagaa
    10441 aaaagcaaac tatgaagaag cctttgttaa agcaatttta gaaaaccata tagataaagt
    10501 ctatgaaaaa tctattgata taaaaaattt tcttgaaaaa ttagaattaa aacatctaga
    10561 gtttaaagat gaaataagaa atttattagt attaatttca tctgaatcct tatattttga
    10621 agatatccca ttaagattat tagattatgc aaatgcaact gatatactta ccgttataat
    10681 cttaattgta aacgtaaaag aatatatttc agaaagtaag gttattcctc ttaatctatc
    10741 taaaaactct tttatgagta cagaggaata taaaaaattt atgaattcta taagtgaatc
    10801 ctatattaga atatatgatt ttgatgaaga ggttagaaaa aaaatagaag aagatcttaa
    10861 actttaagta aaaaggcaac taaaatttta tttaagttgc ctttttataa gaatatttat
    10921 aattaa
//
LOCUS       DAEVGI010000081        10808 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEVGI010000081 DAEVGI010000000
VERSION     DAEVGI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.081"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            96..1835
                     /locus_tag="K8M77_001670"
     CDS             96..1835
                     /locus_tag="K8M77_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter"
                     /protein_id="HBI7086164.1"
                     /translation="MKRKLVALLTVGLAASMLFVACGGGANNTAQGNGNGSESGGTTK
                     DLSKPERIEASNPSALPDAAKNRTDTLIVGTTDPKGEFVPIYSSTLYDLWVNKLVFDG
                     LITNNEKGEPIPNVAESYEVSEDGKTYTFKLNKGIKFTNGQELTAKDVAFTFTSICDP
                     GYDGPRMDAVNNLVGYEEYNKGDASSVEGIKVIDDYTISFTNKNTDAAGIWNFEYGIM
                     PESVYKFEKGNFQAVKDKLLEPVGSGAYKFVHFKPGQEVKFEKNADYWKGEPNIPYIV
                     MKVTNAQTLLQELMAGTVDIDRVGAKPENIDPLKQAGFLNLDLYMQNGYGYMGLNYGS
                     DKVKDPKVRQALLYGLNREGFMQSYYQGYGQVYNSHILPTSWAYNPDVPKYEYNPEKA
                     KELLDEAGWKDTNGNGVRDKDGVELELQWLTYTGSKYVDALIPIVQQSWEQIGVKVTP
                     ELMEFGTMMDKVNNREYDIFNGAWNLSIDPDPSGIFAISQDVPGGFNNIGWRNEEADK
                     LLKEGKGTTNQEERKKAYAEWQLKFSEDVPYILLGNAQEMFASNSRVKGYNPSTYIDW
                     THDVYKLELDNNK"
     gene            1910..2875
                     /locus_tag="K8M77_001671"
     CDS             1910..2875
                     /locus_tag="K8M77_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086165.1"
                     /translation="MLQYITRRILLIIPTIVGVSLVLFFIFAMAPGDAITANMDPNMS
                     VETKQAIREKYHLDEPKTTQYMYWLKGVVTEGSLGESFYYKRPVSEVMKDFIPNSFIL
                     GAASLILGLIIAVPIGIVSATKQYSFFDKFFTVFALMGISMPSFFFGLILIKYLGVQL
                     NLFPISGMTTPGSAYTGMQYVLDVLKHMILPLIVLTLGSVGGWMRYTRSSMLEVVRQD
                     YIRTARAKGLKEKVVIYKHALRNALIPVVTLLGMSIPGLFSGATLTEKIFNWPGIGPV
                     QLSAVNNRDYYLLMGINLTLAILVLIGNLLADIIYALVDPRIRLK"
     gene            2890..3804
                     /locus_tag="K8M77_001672"
     CDS             2890..3804
                     /locus_tag="K8M77_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086166.1"
                     /translation="MQTELNKEVKVKKEKIESPWTVAFKRLKKNKLALGGLFILLVLV
                     LISILGPMLYPHDHLTIDLVRTNQPPSAQHWLGTDESGRDVLARLMYGGRYSLSIGIV
                     AVGISIIIGTTLGILAGYYGGFIDSLIMRIVDVFMCFPFLSLLIMVSAIMSDLKIPPE
                     YRIFVVMFIIGALSWTGTCRMVRGMVLSLREQEFMQAAEALGLSDARKMFLHLLPNTL
                     AVIIVSATLGIGGAILTESSLSFLGLGVTPPTPTWGNMIQTAQDYYTLTNRWWLWIPP
                     GVCIFLTVISINIFGDGLRDALDPKLKV"
     gene            3818..4795
                     /locus_tag="K8M77_001673"
     CDS             3818..4795
                     /locus_tag="K8M77_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086167.1"
                     /translation="MGKTLVEFKNLETHFHTGEGIVKAVNNVSFKIKEGETVCVVGES
                     GCGKSVTAMSLMRLIPSPPGKIVGGEILFEGKDVLKFTEQELMDMRGNDISVIFQEPM
                     TSLNPVFKIGDQICEAIILHQHLSKEEAEKKAIEVLKIIGIARAEEIMKSYPHELSGG
                     MRQRIMIAMAVCCNPKLLIADEPTTALDVTIQAQILDLMRDIKEKLNTSILLITHDLG
                     VVAEMADYVVVMYAGKVIEEAPVLELFKNPLHPYTQGLLKSKPSVNGNETRLYSIPGQ
                     VPNPVGMPDNCYFNERCDRCMEKCKDVQPTLVDVGNRHKVSCWLYKEGK"
     gene            4796..5764
                     /locus_tag="K8M77_001674"
     CDS             4796..5764
                     /locus_tag="K8M77_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBI7086168.1"
                     /translation="MADNTVLKVENLKKYFPIKAGVFSKTVGHVKAVDGVSFTIDKGE
                     TLGLVGESGCGKSTTGRAILRLFEKTDGNVYFEGKDIFALKKKELRDLRPKMQMIFQD
                     PYSSLNPRLSVDSLVGEALLSHKLCSKDELAERVAKTIERCGLARYHMKRYPHEFSGG
                     QRQRIGIARALILNPSFIVADEPVSALDVSIQSQIVNLMMDLQEEMGFSYLFISHDLS
                     IVKHICHKIGVMYLGSLVELAPKNELYDNPLHPYTKALLSAVPIPDPTIKRERIILKG
                     DIPSPANPPSGCKFHTRCPYAMDKCKKEAPEYKCVGKDHFVACHLV"
     gene            5966..6175
                     /locus_tag="K8M77_001675"
     CDS             5966..6175
                     /locus_tag="K8M77_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086169.1"
                     /translation="MFWFKDEITRRKEKEEYEKLMEEVKNKEKPTFKDLVAIMLAQYA
                     IILPMVFGGLIIFAIILYILMAWLK"
     gene            6205..7557
                     /locus_tag="K8M77_001676"
     CDS             6205..7557
                     /locus_tag="K8M77_001676"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBI7086170.1"
                     /translation="MKKGLVVDLSKAAPYLKSHEVAYMQETINQAHNKLHNGTGAGND
                     FLGWVDLPVNYDKDEFARIKEAAKKIQSDSDVLVVIGIGGSYLGARAAIEMLTNNFYN
                     SMSKDKRKTPAIFYAGNNISSSYMADLLKAIDGLDVSLNVISKSGTTTEPAIAFRILK
                     DYMEKKYGKEEAKKRIYATTDAKKGALKTLADAEGYETFVIPDDVGGRFSVLTAVGLL
                     PIAAAGINIDEMMEGAADAREEYANPSLADNECYKYAAARNALYNKGKAIEILVNYEP
                     SVHYFNEWWKQLYGESEGKDNKGLFPAAVDFSTDLHSMGQYIQEGRRDIFETVINVGS
                     PREEIVIEANDENIDGLNFLAGKTMDYVNKQAFRGTLLAHNDGEVPNLVVNVPELTPY
                     YFGRLVYFFEKACGISGYVLGINPFDQPGVEAYKKNMFALLGKPGFEDLKAELEERLK
                     "
     gene            7755..8204
                     /locus_tag="K8M77_001677"
     CDS             7755..8204
                     /locus_tag="K8M77_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBI7086171.1"
                     /translation="MKIIIDGDGCAGRDIIEEVGKKHSVKILIYCTINHMINSDYSEV
                     RMVDGGFQSVDMYVANNTYENDIVITQDYGVAAMALGKGALAISPRGYIYDNDNIDRL
                     LFERHLSQKNRRAGGKSKGNHKRNKEDDDRLYYNLEVLIEKVKAISN"
     gene            8581..10626
                     /locus_tag="K8M77_001678"
     CDS             8581..10626
                     /locus_tag="K8M77_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HBI7086172.1"
                     /translation="MGELLLELDTRDIIEHNSPVNIKIINNTNDNLRFKILIGKDGIW
                     ETLREFEKEDEFIWTPKEEGEYMIMVQGRKEDSNKPFDYKVTEGVNVKRTVHDLQDEM
                     NKDEIKEEKLIKGIYLNKDVFNVGEKAELKVDSNNENIMYRYFISGKNGWQLIKDYTL
                     ENKLNYTINESGQFEFLIECKKTSSTDTTDDYATVIFTSKEFVKPEIINFTNLSEKLI
                     VNSDLTFEVEANFEDERTALYKFVKISPDGSRTCIQDFSSSSIVTFKEFKEGRYKLLC
                     LIRDMYSNKEFDDRAVMVYDIKPYEPLQLRSFTTDLSSPQEKGEVIEIKAVIDGGSDI
                     LYRFKIDGEISEDTGYTRNSTLKWNAKKEGNYRITLWAKDESFIGDYEIESKLDYKIE
                     KKVKKPIKITELIFDRDKTPLINEVIKLRVSAEGGTDLKYSFLIIKDGKNIESIDFGS
                     NNWVEFIPNEKGNYQLDVRVKDKYSTKEYDVHTVLNFKVREYAEGKIDHILVPAKEYF
                     LVGDDIDIEVIVQNTRQVKVKYVTKVNGQVVEETDYVKNKRFKVTPKCPGKYTIDIFA
                     RNIKCKEGFDSRREVRFYVSEALPITDTKISTSDKVFKINEEINFSTKCEGGSRVCYE
                     YYIMVNGNWSLVQKYSRKSYYSFRPYVPGKYKVLVLTKSYYKKCAYEDYDTFEFKVE"
BASE COUNT         4205 a         1186 c         2049 g         3368 t
ORIGIN      
        1 cattaaatgt atgttattgt aacaaaattt tttatatggg gcattttaaa tttgttacgt
       61 aacagcaata aatatattaa aagggggcag attaaatgaa aagaaaatta gttgcattat
      121 taacagtagg attagcagct tcaatgttat ttgtagcatg tggtggagga gctaacaata
      181 cagctcaagg aaatggtaat ggttcagaat caggaggaac tactaaggat ttatcaaagc
      241 cagaaagaat agaggcatca aatcctagtg cacttccaga tgctgcaaaa aatagaactg
      301 atactttaat agtaggaact acagatccaa agggtgaatt tgttccaata tattcttcta
      361 ctctttatga tttatgggtt aacaagttag tatttgatgg attgattact aataatgaaa
      421 aaggtgaacc aattccaaat gtagcagaaa gttatgaagt ttctgaggat ggaaaaactt
      481 atacatttaa attaaataag ggtattaaat ttactaatgg tcaagaatta acagcaaagg
      541 atgtagcatt tacatttact tctatttgtg atccaggata tgatggacca agaatggatg
      601 ctgtaaataa tttagttgga tatgaagagt acaataaggg cgatgctagt agtgttgaag
      661 gtataaaggt tattgatgat tatacaatat cattcactaa caagaatact gatgcagctg
      721 gtatatggaa ttttgaatat ggaattatgc ctgaaagtgt ttataaattt gaaaaaggaa
      781 acttccaagc tgttaaggat aaattattag agccagtagg ttcaggtgct tataaatttg
      841 ttcactttaa accaggacaa gaagttaagt ttgaaaaaaa tgctgattac tggaaagggg
      901 agccgaatat tccttatata gtaatgaaag ttacaaatgc acaaacatta ttacaagaat
      961 taatggctgg aacagttgat atagatagag ttggtgctaa accagaaaat atagatccat
     1021 taaaacaagc tggattctta aacttagatc tttatatgca aaatggttat ggatacatgg
     1081 ggcttaacta tggaagtgat aaggttaaag accctaaagt aagacaagcg ttactttatg
     1141 gattaaatag agaaggattc atgcaatctt attaccaagg atatggtcaa gtttacaact
     1201 cacacattct tcctacttca tgggcatata acccagatgt tcctaagtat gaatacaatc
     1261 cagaaaaagc taaagaatta cttgatgaag caggctggaa agatacaaat ggaaatggag
     1321 ttagagataa ggatggagtt gaattagaac ttcaatggtt aacttatact ggttctaaat
     1381 atgttgatgc tttaatccca atagttcaac aatcttggga acaaataggt gttaaagtta
     1441 ctccagaact tatggaattt ggaacaatga tggataaagt taataacaga gaatatgata
     1501 tattcaatgg tgcttggaac ctttcaatag atccagaccc atcaggaata tttgcaattt
     1561 ctcaagatgt accaggcgga tttaataata ttggatggag aaatgaagaa gcagataagt
     1621 tattaaaaga aggtaaagga acaacaaatc aagaggaaag aaagaaagct tatgctgaat
     1681 ggcaattaaa attctctgaa gatgtacctt atattcttct tggaaatgca caagaaatgt
     1741 ttgcatcaaa ttcaagagtt aaaggatata acccttcaac ttatatagat tggactcacg
     1801 atgtctataa acttgaatta gataacaata aataaaattt aaaataaatt aagaaattag
     1861 cctggacaaa aaatccaggc taattttgaa aaatgggggg aacagcaata tgctacagta
     1921 tatcactaga agaatactat taataatacc gactattgtt ggtgtatcat tagtactttt
     1981 cttcatattt gctatggctc caggagatgc tattacagca aatatggatc caaatatgag
     2041 tgtagaaaca aaacaagcaa taagagaaaa atatcattta gatgagccaa aaacaactca
     2101 gtacatgtat tggttaaaag gcgttgtaac agaagggtct ttaggagaat ctttttacta
     2161 taaaagacca gtttcagaag taatgaaaga ctttatacca aattccttta ttttaggagc
     2221 ggcatcacta attttggggc ttataattgc ggtgccaata ggtatagttt cagcaacaaa
     2281 gcaatattca ttttttgata aattttttac ggtatttgcc cttatgggaa tatcaatgcc
     2341 atcatttttc tttggactta tattaattaa atatttagga gttcaactta atttatttcc
     2401 aatatcgggt atgactactc caggaagtgc atatacagga atgcaatatg ttctagatgt
     2461 attaaaacat atgattttac cacttatagt tttaactctt ggaagtgtag gtggatggat
     2521 gagatacaca agatcaagta tgcttgaggt agttagacaa gattatataa gaactgctag
     2581 agcaaaagga ttaaaagaga aggtagttat atataaacat gctttaagaa atgcacttat
     2641 accagttgta acactattag gaatgtcaat tccaggatta ttctcaggtg ctacattaac
     2701 agaaaaaatc tttaactggc caggaatagg tccagtacag ttaagtgcag ttaataacag
     2761 agattattat ctacttatgg gtataaacct tactttggct atattagttt taataggaaa
     2821 cttacttgct gatattatat atgcattagt agacccaaga ataaggctta agtaggaggt
     2881 gctttttata tgcaaacaga attaaataaa gaagttaaag tgaaaaaaga gaaaatagaa
     2941 agcccttgga ctgtagcttt taaaagattg aaaaaaaata agttagcttt aggtggctta
     3001 tttatattgt tggtcttagt tttaatttca atattaggac caatgttata tccacatgat
     3061 catttaacca tagatttagt aagaactaat caaccaccat cagcgcagca ttggctagga
     3121 actgatgaat caggaagaga tgttttagct agacttatgt acgggggaag atattcactt
     3181 tcaataggta tagttgcggt aggaatttct ataattatag gaacaacttt agggatatta
     3241 gcaggatatt atggaggatt tatagatagt ttaattatga gaattgttga cgtatttatg
     3301 tgtttcccat tcttatcatt attaataatg gtatctgcta taatgtcaga tttaaaaata
     3361 ccaccagagt atagaatttt tgtagttatg tttattatag gggcactgag ttggactggt
     3421 acctgtcgta tggttagagg tatggtctta tctttaagag aacaagaatt tatgcaagcg
     3481 gcagaagctt taggtctatc agatgctaga aaaatgtttt tacacttatt acctaataca
     3541 ttagcagtta ttatagtttc agctacatta ggaattggtg gagctatatt aactgaatca
     3601 tctttaagtt tcttaggtct tggagttaca cctcctacac caacttgggg taatatgata
     3661 caaacagctc aagattacta tacattaaca aatagatggt ggttatggat acctccagga
     3721 gtatgtatat tcttaactgt tatatctata aatatttttg gagatggatt aagagatgct
     3781 ctagatccta agctaaaggt ctaagggggc gagtgtaatg ggaaaaacat tagtagaatt
     3841 taaaaatctt gaaactcact ttcatacagg tgagggaatt gttaaagcag taaataacgt
     3901 tagttttaaa ataaaagaag gggagacagt ttgtgtagtt ggagaatctg gatgcggaaa
     3961 atctgttact gccatgtctc ttatgagatt aataccaagt cctcctggaa aaatagttgg
     4021 aggagaaatt ctttttgagg gaaaagatgt tttaaagttc acagagcaag aacttatgga
     4081 tatgagagga aatgatatat cagttatttt ccaagaacct atgacatcat taaacccagt
     4141 atttaaaata ggagatcaaa tttgtgaagc aataattctt catcagcatt tatcaaaaga
     4201 agaagctgaa aagaaagcta tagaagtttt aaaaataatt ggtatagcta gagcagaaga
     4261 aataatgaaa tcttatcctc atgaattatc aggaggaatg agacaaagaa ttatgattgc
     4321 tatggcagtt tgttgtaatc ctaagctttt aatagctgac gaacctacaa ctgcccttga
     4381 tgttacaatt caagcacaaa ttctagatct tatgagagat attaaagaga aattaaatac
     4441 atcaatatta ttaataacac atgacttagg agtagttgct gaaatggctg actatgttgt
     4501 tgtaatgtat gctggtaagg ttattgagga agctccagtt ttagagcttt ttaaaaatcc
     4561 tcttcatcca tatacacaag ggttacttaa atctaagcca tcagttaatg gaaatgaaac
     4621 tagactttat tcaataccag gacaagttcc taatccggta ggtatgccag ataattgcta
     4681 cttcaatgag agatgtgata gatgtatgga aaaatgtaag gatgttcaac ctactcttgt
     4741 tgatgtagga aatagacata aagtttcatg ctggttatat aaggagggaa agtagatggc
     4801 agataataca gttttaaaag ttgaaaattt gaaaaaatac tttccgatta aagcaggagt
     4861 tttttctaag acagttggac atgtaaaagc tgttgatgga gtaagtttta ctatagataa
     4921 gggtgaaact ttaggattag ttggagaatc aggttgtgga aaatcaacaa caggaagagc
     4981 aattttaaga ttatttgaaa aaacagatgg aaatgtatat tttgaaggaa aagatatttt
     5041 tgctcttaaa aagaaagaac ttagagattt aagaccaaaa atgcaaatga tattccaaga
     5101 tccatattca tcattaaatc ctagattatc agtagattct ttagtaggag aggctctttt
     5161 aagtcataag ttatgtagta aagatgaatt ggctgaaaga gtagctaaaa ctatagaaag
     5221 atgtggactt gctagatatc atatgaaaag atatccacat gaattctcag gtggacaaag
     5281 acaaagaata ggtatagcaa gagccttaat tttaaatcca agttttatag ttgcagatga
     5341 acctgtttca gcactagatg tttctataca atcacagatt gtaaacttaa tgatggattt
     5401 acaagaagaa atgggatttt catatctttt catatctcat gatttaagta tagtaaagca
     5461 tatttgtcat aaaataggtg taatgtactt aggtagctta gttgaattag ctcctaaaaa
     5521 tgaactttat gataatccat tgcatccata tacaaaggca ttattatcag cagttccaat
     5581 accagaccct acaattaaga gggagagaat aatattaaag ggtgatattc cttcaccagc
     5641 taatccacca agtggctgta agttccatac tagatgtcca tatgctatgg ataaatgcaa
     5701 gaaggaagcg ccagaatata agtgtgttgg aaaagatcac tttgttgcat gtcacttagt
     5761 ttaatctaaa attaggttta tttattaaaa tgtaaaaatt gaattaaaaa attaaatttt
     5821 atgtagtgta ggggattaaa ttctcctaca ctataactat atataataaa atttcattaa
     5881 atatataaaa agttttatat tgtattgtaa tccataaata ataaatgata aaatattaac
     5941 gtgtggtaaa cagggggaat taagtttgtt ttggtttaaa gatgaaataa ctagaagaaa
     6001 agaaaaagaa gaatatgaga aattaatgga agaagttaaa aataaagaaa agccaacttt
     6061 taaggatttg gttgctataa tgttagcgca atacgcaata atacttccaa tggtttttgg
     6121 aggcttaatt atttttgcga ttatattata tatattaatg gcgtggttaa aataaattta
     6181 taaacgctaa tggaggtagt aataatgaaa aaaggattag ttgttgattt atcaaaagca
     6241 gctccatatt taaaatctca cgaagttgct tacatgcaag aaactataaa ccaagcacac
     6301 aataagttac ataatggaac aggtgcagga aatgactttt taggatgggt tgacctacca
     6361 gttaattatg ataaagatga gtttgctaga ataaaagaag ctgctaagaa aatacaaagt
     6421 gattcagatg tattagttgt tataggaata ggtggttctt acttaggagc aagagctgct
     6481 atagaaatgt taactaataa cttctataac tctatgtcta aggataagag aaaaactcca
     6541 gctatattct acgcaggaaa taacataagc tcaagctaca tggctgattt attaaaagct
     6601 attgatggat tagatgtaag cttaaatgtt atatcaaaat caggtactac tacagaacct
     6661 gctatagctt tcagaatctt aaaagattac atggaaaaga aatacggaaa agaagaagct
     6721 aaaaagagaa tatatgctac aacagacgct aagaaaggtg ctttaaaaac tttagctgat
     6781 gctgaaggat atgaaacttt cgtaattcca gatgatgttg gaggaagatt ctcagtatta
     6841 acagcagttg gattattacc aatagctgca gctggaataa acatagatga aatgatggaa
     6901 ggagctgctg atgctagaga agaatatgct aatccttcat tagctgataa cgaatgttac
     6961 aaatatgcag ctgctagaaa tgctttatac aataaaggta aagctataga aatattagtt
     7021 aactatgagc catcagttca ttacttcaat gaatggtgga aacaattata tggagaatca
     7081 gaaggaaaag acaacaaagg attattccca gctgctgttg atttctcaac tgatttacac
     7141 tcaatgggac aatacatcca agaaggtaga agagatatct tcgaaacagt tattaatgta
     7201 ggatctccaa gagaagaaat agtaatcgaa gctaacgatg aaaacataga tggattaaac
     7261 ttcttagctg gaaaaactat ggactatgtt aacaaacaag cattcagagg aactttactt
     7321 gctcataatg atggagaagt tccaaactta gttgttaatg ttccagaatt aactccatac
     7381 tacttcggaa gattagttta cttcttcgaa aaagcttgtg gaatcagtgg atatgtttta
     7441 ggaataaatc catttgacca accaggtgtt gaagcttaca agaaaaacat gtttgcttta
     7501 cttggaaaac caggttttga agatttaaaa gctgagttag aagaaagatt aaaataatat
     7561 aaatttaatt ataaaaagga gctcttttta gagcttcttt tttgttttaa gagtataatt
     7621 ttattaatat ataaattatt cattatttta atagtattag taaattaata agaaagtgta
     7681 taattttatt aatgtataat taacaaatat tcaaaaaaag ctagtagaga atatattatg
     7741 ggaggattat aaaggtgaaa ataattattg atggtgatgg atgtgcagga agagatataa
     7801 ttgaagaggt tggtaaaaag cattcagtta aaatattaat ttattgcaca ataaatcata
     7861 tgataaattc agattactct gaggtgagga tggttgatgg tggatttcaa agcgtagata
     7921 tgtatgtggc taataatact tatgaaaatg atattgtaat aactcaagat tatggagttg
     7981 cagctatggc tttgggaaaa ggagctttag ccataagccc tagaggatat atttatgata
     8041 atgataatat tgacagatta ctttttgaaa gacatttaag tcaaaaaaat agaagagctg
     8101 gtgggaaatc aaaaggaaat cataaaagaa ataaggaaga tgatgataga ctgtattata
     8161 atttagaagt tttgatagaa aaggtaaagg ctatttcaaa ttagatataa taagataagg
     8221 tttgaaaaat taaaaataac tttggaaaat ttaaaaataa acttttataa ttagaaaata
     8281 aatttaaaaa ataatatata tactattaat taagatatat taaaaaaaag aaatctcaga
     8341 gtggctgaga tttctttaat ggtttgatta tatggatata ataaaattag gagatttaca
     8401 gtgttaagta acactaccta tattatagac agttttaaac tttttataca ttttataaaa
     8461 gtagaaaaaa tgtagaaata gagtgtttta tggtaagaaa aatagaaaat atttacataa
     8521 aaacagaagt ataatataat ttatttatct ataatgtttc gggttttagg gggagtgatt
     8581 atgggagaat tacttttaga gttagatacg agagatataa tagaacataa tagtccggtt
     8641 aacataaaaa taataaataa cactaatgat aatttaagat ttaaaatttt aataggaaag
     8701 gatggtattt gggaaacttt aagagagttt gaaaaagaag atgaatttat atggactcct
     8761 aaagaagagg gagagtatat gattatggtt caaggaagaa aagaggattc aaataagcct
     8821 tttgattaca aagttactga gggggttaac gtgaaaagaa cggttcatga ccttcaggat
     8881 gaaatgaata aagatgaaat taaagaagaa aaattaataa aaggaatcta tctaaataaa
     8941 gatgtattta atgttggaga gaaggcagag ttaaaggttg atagtaacaa tgaaaatatt
     9001 atgtatagat attttataag tggaaagaac ggatggcaac ttataaagga ttatacatta
     9061 gaaaataaat taaattatac tattaatgaa agtggtcaat tcgagttttt aattgaatgt
     9121 aaaaaaacaa gttcaactga tacaacagat gattatgcta ccgttatatt tacatcaaaa
     9181 gaatttgtaa agcctgaaat aattaatttt actaatttaa gtgaaaaatt aatagtaaac
     9241 tcagatctta cctttgaagt ggaagcaaat tttgaagatg aaagaaccgc attatataaa
     9301 tttgtaaaaa tttcacctga tggaagtaga acttgtattc aagatttttc atcaagcagt
     9361 atagttactt ttaaagaatt taaagaggga agatataagc ttttgtgttt aataagagat
     9421 atgtattcta ataaggagtt tgatgataga gctgttatgg tatatgatat aaaaccatat
     9481 gaaccattac aattaagaag ttttactaca gatttaagct cacctcaaga aaaaggagaa
     9541 gtaatagaga taaaagctgt tattgatggg ggaagtgata tcctctatag atttaaaata
     9601 gatggagaaa tatctgagga tacggggtat acaagaaata gtactttaaa atggaatgct
     9661 aaaaaagaag gaaattatag aatcactctt tgggctaagg atgaatcctt tattggagat
     9721 tatgaaatag aatctaaatt agattataaa atagaaaaaa aggtgaaaaa gccaataaaa
     9781 ataacagaat taatatttga tagagataag acacctttaa taaatgaagt aataaaactt
     9841 agggttagtg cagagggtgg tactgactta aaatattcct tcttaataat taaggatggt
     9901 aaaaacatag aaagtattga ttttggaagt aataattggg ttgagtttat tccaaatgaa
     9961 aaaggaaatt atcagcttga tgtaagagta aaagataagt attcaacaaa ggaatatgat
    10021 gtgcatactg tactaaattt taaggttagg gagtatgcag aaggaaaaat tgatcatata
    10081 ttagttccag ctaaggaata ttttttagtt ggagatgata tcgatattga agtaatagtt
    10141 caaaatacta gacaggtaaa ggttaagtat gttactaagg taaatggaca agtagtagaa
    10201 gaaactgatt atgtaaaaaa taagaggttt aaagttacgc caaaatgtcc aggaaaatac
    10261 acaattgata tatttgctag aaatattaaa tgtaaagagg gatttgattc aagaagagaa
    10321 gtaagatttt atgttagtga agctctacct attacagaca ctaaaataag tacttctgat
    10381 aaagtcttta aaattaatga agagataaac ttttctacaa aatgtgaagg tggaagtaga
    10441 gtttgctatg aatattatat aatggttaac ggaaattggt ctttagttca aaaatatagt
    10501 agaaagagtt attattcttt tagaccatat gtaccaggaa agtataaagt tttagtatta
    10561 actaaaagtt attataaaaa atgtgcttat gaggactatg atacttttga atttaaggta
    10621 gaataaaata ttaaagttca atatatgttt agggctctat tttacataat attaatttta
    10681 agataagctt ttaagactaa tattagtttt aaattatttg aatttaatat ttatttaaaa
    10741 tagagtccta tttattttta tagtttcaat agaactttta aaaggaaaaa aagagaatat
    10801 ttaaaaaa
//
LOCUS       DAEVGI010000082        10791 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEVGI010000082 DAEVGI010000000
VERSION     DAEVGI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.082"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            10..999
                     /locus_tag="K8M77_001679"
     CDS             10..999
                     /locus_tag="K8M77_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBI7086173.1"
                     /translation="MSNQLRSGKNNKYLKRRIAVAIAGIVIIAGVGFGIKSIVSNKAN
                     KEALASAKKEEAKKEEEKPKPTDIMPNGNIIYAADSYAVSADEVEKMLEGKASNNDKE
                     IFLTFDDGPSENTREILKILKEEDVHATFFDIGSALKDNKENQELLKQEIDQGNAVAG
                     HSFSHNYKTLYPGNSVDVNKFMNELNETNEIMKSVLGKNFNARVIRMPGGYMSRRYYR
                     DPNLKALDEAFAKDNIVSIDWDAETGDATGRYYTVEQYVQNSAKNINTLNHVILLMHD
                     AAAKKETVQALPAIIKFYKEHGYAFKVIKNSPVGEKNSSDSTKGSQNTDNKTK"
     gene            1210..2271
                     /locus_tag="K8M77_001680"
     CDS             1210..2271
                     /locus_tag="K8M77_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRK06851 family protein"
                     /protein_id="HBI7086174.1"
                     /translation="MKNVVYYYGAGNTAKGFRPLYNSIFQGIKKIYSLNGGSNIFKTA
                     IIKELIDEYSDTLSLEGIVSTIDNNEIEGVILREKEIAVINGTPLHGFMNLDSDNIEV
                     IDLDVFLDKDEIEKNEETIEFLKEQFKECMENAHKEYEKALKIHDYWEDIYFPYLNIE
                     KANKFTDHVISLLTNGAVKKESKGKCVERYLGGATPSGAKDSVPSITEGVKRYFLKGR
                     AGTGKSTMLKKIAKAVMELGYDVEVYNCGFDPDSKDMVISRELNFAIFDSTAPHEYFP
                     SREDDEIIDVYTTYIDGDVDKIHKEEIKKIALNYKDQVTKGTAFLLKGEEVKNKLDDI
                     YNNAFNHKEAKSALEFLFE"
     gene            complement(2322..3416)
                     /locus_tag="K8M77_001681"
     CDS             complement(2322..3416)
                     /locus_tag="K8M77_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBI7086175.1"
                     /translation="MNERNHGLGIIALESCTELGNAIDKLIQEKRNCEESFLIKTDEI
                     RFSNGEGKVKIPESVRGRDIYILCDIGNYSCTYKMFGFENHKGPDEHFQDIKRTVSAI
                     RGKARRITVIMPLLYESRQHRRKGRESLDCALALQELERLGVDEVLTFDVHDPNVQNA
                     IPLMSFENFYPTYDIVKSLIHNEKDLELDKEKLLVISPDTGAMDRAIYYSSVLGVDVG
                     LFYKRRDHSRIVNGKNPIVKHEYMGRDVDGKDVLIVDDMIASGESVLDIAKELKGRNA
                     RNVYVATTFSFFTEGLEKFNKFFNDGIIKSVYSTNLTYIPDELKTAPWFKAVDLSDFI
                     ARIINRLNYDKSVASYMDATHIIQRLLDEK"
     gene            3826..5814
                     /locus_tag="K8M77_001682"
     CDS             3826..5814
                     /locus_tag="K8M77_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBI7086176.1"
                     /translation="MEVLSHGCTLDCFDCCKFNVYKEGSEILKIEGDKDHPFTKGLIC
                     KKGIAHLKRLNHKDRIYTPLLKNNGVWKEISFEDALEIMKEKLESTKEKYSSKSILYY
                     SQYGSGGILKGIEDIFFNFYGGVSKATGGPCWSAGMRAQKYDFGDSVSNSLEDMINSK
                     NIFLWGKNPANTTIHTMAILNKAKKNGSRIIVIDPINTQSVKFGDIHVKIKPGTDGAL
                     AMAMAKIIISKDLQDKDFINKYVLGFQEYKNHLDNFDLDYLSYECGIEIEDIENLTKY
                     YCEKNSSIYLGYGMQKYKNGGNTIRAIDALGALTGQIGVKGGGVNYANKVLSRILDLD
                     PFKSEEIGENREFYVSNINEFIEEPKKYSLSVEDSNAPIKIMVIANSNLMNQLPNLNR
                     LNNSIDKVEFKVCFDMFMTYTASKCDLFIPCTNTLESEDMVFSSMTNPYLIYNEKITE
                     PREKLMDEYYFFRELAKRMNLKGYPILSKKEYLSKVIEPLMRYNKYITLDYLKNNPFT
                     VYDDVAWENKKFKTPSGKFELASKRALKECGSLTPTYISPRIKENCFRLLTNHSKDSL
                     SSQHYIDVDEKAKVYLNENMIRKFSLICGEKVKLKSRTGEITAICSLDNGVQDYVALM
                     YVGWWKKHGNPNFLTESGISDIGGQITYNETFIEIENI"
     gene            5911..9312
                     /locus_tag="K8M77_001683"
     CDS             5911..9312
                     /locus_tag="K8M77_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7086177.1"
                     /translation="MVNKEIIENGFFKIEKKLNEEFIGQKTFNKELCDYFKEKIEEDS
                     KGILLIVEERDIFKNSVLKYFFKELNYYKFVKNSKIDEIDLASYKFNLGYNAFLTDLY
                     EKLNNDSQCLVFKNTDKASEDILNVLSGISPNSCIMLKDNYVIKNKFLVEAEEIDEDI
                     VNKIICNNKFFIFLYNKDKGEELEIIDNEFLINKDKTLYRKALSEKDKNKVIKKKLLK
                     EINKVKELFDIDIIIGVDENPYVEEKSGVCTYIEDNFREKGSLSLDQYISYKICKPII
                     NIIRKEAIEANSRLLIYVEDDEIYCKSNDEVYDLSKYSNPTLEEVRYKLESIIGMKEL
                     KEFIDKIENNFKVQKIREKLGLKTTQISLNMIFAGNAGTGKTNAARITFEYLYALGMI
                     KENIFKEVSKADFIGEGISNTVRRTNEIIESALGGVLFIDEAYSLCTDKNDKVGREIV
                     DALLKGIEDNRDNITVILAGYEKDMETFLSFNQGLKSRFPNVIKFEDYNPSEMYEIAV
                     NIAKSKGYRIAKSAKAGLIELFTKNQMVGKSDLGNARFVRNIVENAIMDASKKYLVNK
                     ERAIDLLEKDNFNFKVSAKFDLEEKLKEIIGLEEVKEFLRSQYKLIVAQEKRKSVGVN
                     TKIEQNLNMIFAGNPGTGKTSIARLVAEMLNSMGLLKVGQLVETDRSSFVSDIPGETS
                     KKTEEKFKEALGGVLFIDEAYTLANDSIGREAIETLLKLIEDYSREVIVILAGYEEEM
                     ENFFDVNIGLRSRFPLWTKFEDYNPNELLEMAIKLVEAKGFKLSKNGYSELKKNFVEI
                     YENADAQSGNGRMVRNYVEQLIRNQSIRIAESDISVYEMNLINTKDIKAMNAIKYDNH
                     FDLEKKLNDLIGNEELKDFLRGQYKLMKIREKRKKLGFQVDLNKYMNMVFTGESGTGK
                     KTVLNIYSEMLYSMGIIKAKNIVQIDKYEFMAMINSGMKVEDILNKHVGKILYIDKWN
                     TFFGEERYNEIVSDLIKFIDNNSNRIVIVLGGIRGKMKDLILTNEGLNYRFPVWVDFG
                     NYNERELYDIASSTLVKKGFTLDEEAETALENAIGDIQKRMGELALKNGLMIKQFLDS
                     LVRVQSIRVCDEDFDINTINRIISEDILKSTEIFLKKNTTQNGSF"
     gene            9668..10759
                     /locus_tag="K8M77_001684"
     CDS             9668..10759
                     /locus_tag="K8M77_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBI7086178.1"
                     /translation="MKVLCGTIVSTFMFGMATPVFAQSEKTEAVVTLGANLTKSERLQ
                     MLDAFGVKANEVKIIDVTNQDIREQLGLDTSKPIPASSQSISSSYVVVKDKGGINVTT
                     NNLTEVTGSMLANALLTSGVNNADVKADAPFKVTGTAALAGILKGFEDASGEELSLPK
                     KEAAREEISLTNNLSNAKTKDGQTLGKDEAAVVVNDIKTDVIKDKPKNDEEIGKIVNN
                     VTNNYNILLTQGQQEQTIKFMSKINDLDYNYGAMKESLNQMNDKLQQILKDTGKQLEE
                     SGLLEKALNGIKNVLVDIKDFLVNMFSSASEKVKDGITYDENGNIVIKTGNNSDESKN
                     QENIQDKPQTQSNDNNQNQQNEQGQNQTK"
BASE COUNT         4438 a         1089 c         1879 g         3385 t
ORIGIN      
        1 atgaattttg tgagtaatca attaagaagt ggaaaaaata ataagtattt aaaaagaaga
       61 atagctgtag ctatagctgg tattgtaata attgcaggag ttggttttgg aattaaatca
      121 atagttagca ataaagctaa taaagaggct ttagcatcag ctaaaaaaga agaagctaaa
      181 aaagaagagg aaaaaccaaa accaacagac ataatgccaa atgggaatat aatttatgct
      241 gcagattcat atgcagttag tgcagatgaa gttgaaaaaa tgcttgaagg aaaagcttca
      301 aataatgata aagaaatatt tttaactttt gatgatggtc caagtgaaaa tacaagagaa
      361 atattaaaga ttttaaagga agaagatgta catgctacat tttttgatat aggatctgcc
      421 ttaaaggaca ataaagaaaa tcaagagtta ttaaaacaag aaattgatca aggaaatgca
      481 gtagctggtc atagtttttc acataattat aaaacattat atccaggaaa ttcagttgat
      541 gtaaataagt ttatgaacga attaaatgaa actaatgaaa taatgaaaag tgtattagga
      601 aaaaacttta atgctagagt aattagaatg ccaggtggat atatgtctag aagatattat
      661 agagatccta acttaaaagc tttagatgag gcttttgcaa aggataatat agttagtata
      721 gattgggatg cagaaactgg tgatgcaact ggaagatatt atacagtaga gcaatatgtt
      781 caaaactcag ctaaaaatat taatacttta aatcatgtaa ttttattaat gcatgatgca
      841 gcagctaaaa aagaaactgt acaagcatta cctgcaataa ttaaattcta taaagaacat
      901 gggtatgcat ttaaagtaat aaaaaattca cctgtaggtg aaaaaaattc ttcagattca
      961 actaaaggtt cacaaaatac tgataataaa acaaagtaaa tataaattat tagcaataaa
     1021 catacattga atttttaaat attaatactt aattaaatat ataagaagga tttaagaaaa
     1081 aattatttaa gataaagaag agagttgatt ttataaaaat aagatcagct ctcttattta
     1141 ttgtaactta tttacaaatt taaaaatata ataagttaag agctatatat tgatattaag
     1201 gagaagaaga tgaaaaatgt agtttattat tatggagcag gaaacacagc aaaagggttt
     1261 agaccacttt ataattctat atttcaagga atcaagaaaa tttattcact aaatggaggt
     1321 agcaatatat ttaaaactgc aattataaag gaacttatag atgaatatag tgatacttta
     1381 agtttagaag gaatagttag cactatagac aataatgaaa tagagggagt aatattaaga
     1441 gaaaaagaaa tagcagtaat aaatggaaca cctcttcatg gatttatgaa tttagatagt
     1501 gataatattg aagttataga tttagatgtg tttttagaca aagatgaaat agaaaaaaat
     1561 gaagagacta tagaattttt aaaggaacaa tttaaagagt gtatggaaaa tgcccataaa
     1621 gaatacgaaa aggctttaaa gatacatgat tattgggaag atatttattt cccttactta
     1681 aatattgaaa aggcaaataa atttactgat catgttattt ctttattaac taatggtgca
     1741 gttaagaaag agtcaaaggg taagtgtgtt gaaagatatt taggtggagc aacaccaagt
     1801 ggagctaagg attctgttcc tagtataact gaaggggtaa aaagatactt tttaaaagga
     1861 agagcaggaa caggaaagtc aactatgcta aagaaaatag ctaaggcagt aatggaatta
     1921 gggtatgatg tggaagttta taattgtgga tttgatccag atagtaagga tatggttata
     1981 agtagagagt taaactttgc aatttttgat agcacagctc ctcatgaata tttcccaagt
     2041 agggaagatg atgaaataat agatgtttat acaacataca ttgatggaga tgttgataaa
     2101 attcacaaag aagagattaa aaagatagct ttaaattata aagatcaagt gacaaaggga
     2161 acggcttttt tattaaaagg agaagaagta aaaaataagc tagatgatat ttataataat
     2221 gcatttaatc ataaggaagc aaaaagtgct ttagaatttt tatttgaata gactaaaaag
     2281 gtgctctgca aaaaaacgca gagcactttt aaattaagta attatttttc gtctaataat
     2341 ctttgaatta tgtgagtagc gtccatatat gatgctacag atttatcata gtttaatctg
     2401 tttattattc ttgcgataaa atcagataaa tcaacagctt taaaccaagg agcagtcttt
     2461 aattcatcag gtatataagt taaatttgtt gagtaaacgc tcttaattat tccatcatta
     2521 aagaatttgt tgaatttttc taaaccttca gtaaagaatg agaaagtagt agcaacatat
     2581 acatttctag catttcttcc tttaagttct ttagcaatat caagtacaga ttctccagaa
     2641 gctatcatat catcaacgat taagacatct tttccatcaa catctcttcc catatattca
     2701 tgtttaacta ttgggttttt accattaact atacgtgaat ggtctcttct tttgtagaat
     2761 aaaccaacat caacacctag tacgcttgaa tagtagattg ctctatccat agcaccagta
     2821 tcagggctta ttacaagtaa tttttcctta tcaagctcta aatctttttc attgtgaatt
     2881 aaagatttaa ctatatcata agttgggtag aagttttcaa aggacattag tgggatagca
     2941 ttttgaacgt tagggtcgtg aacatcaaaa gttaaaactt catcaacgcc taatctttct
     3001 agttcttgaa gtgctaaagc acaatctaga gattctcttc ctttacgtct atgttgtctt
     3061 gattcatata aaagaggcat tattacagtt attcttctag cttttcctct tatagcagaa
     3121 actgttcttt ttatgtcttg gaaatgttca tctggtcctt tatgattttc aaaaccaaac
     3181 attttatatg tacaactata gttacctata tcacaaagaa tgtatatatc tcttcctctt
     3241 acagattcag gtatttttac tttcccctca ccgttagaga atcttatctc atcagttttt
     3301 attaaaaaag attcctcaca atttcttttc tcttgtataa gcttgtctat agcattacca
     3361 agttctgtac aactttctaa agctataata cctaatccat gatttcgttc gttcattcgt
     3421 ctatctcctt ttttaatgtt tatttaaaat attttacaat cgtaaatact aaagatatcg
     3481 tcatttatat tatatacgaa taatttactt ttaaaaatag aaatttatca taatttttta
     3541 ctaagtttaa taaaaaactt gaaattttgt tgtcagataa tttagatttt ttaaaagata
     3601 tgaatattat tctaaattta attcataaaa aatctctttc tttacagtta aatttaagtt
     3661 gaaagagagt aaaaaggtca tagtataatt ataattattt ttattttgta atagtaattt
     3721 ttgattttat ggttgttaaa taaggaacat tcatataaaa ttagactaaa ttagaaatag
     3781 aggaatttta ttatgtatgt catttaaaga gaaggggaag ttattatgga agtattaagt
     3841 catggatgta cattagattg ttttgattgt tgtaaattta atgtttataa agaaggaagt
     3901 gaaattctaa aaatagaagg tgacaaagat catccgttta caaagggact catatgtaaa
     3961 aaaggaatag ctcacttaaa aagattaaat cataaggata gaatatacac tcctctttta
     4021 aagaataatg gagtatggaa agagatttcc tttgaagatg ccttagaaat aatgaaagag
     4081 aaacttgaat ctacaaaaga gaagtattct tctaagtcaa tattatatta tagccaatat
     4141 ggaagtggag gaatactaaa gggaatagag gatatattct ttaattttta tggtggtgta
     4201 agcaaagcta caggaggtcc ttgctggagt gctggaatga gagctcaaaa atatgatttt
     4261 ggagattctg tatcaaattc tttagaggat atgataaata gtaaaaatat atttttatgg
     4321 ggtaaaaatc ctgcaaatac aaccatacat actatggcaa ttttaaataa ggcaaagaaa
     4381 aacgggagca gaataatagt tatagatcca ataaatactc aaagtgtaaa gtttggggac
     4441 attcatgtaa aaattaaacc tggtacagat ggagctttag ctatggccat ggcaaaaata
     4501 ataatttcta aagatcttca agataaggat tttataaata aatatgtttt aggatttcaa
     4561 gaatataaaa atcatttaga taattttgat ttagattatt taagttatga atgtggaata
     4621 gaaatagagg atatagaaaa tctaactaaa tactattgtg aaaaaaattc cagcatatat
     4681 ttaggatatg gaatgcaaaa atataaaaat ggtggaaata ccataagagc tattgatgcc
     4741 ttaggtgctt taactggtca aattggagtt aaaggtggtg gagtaaatta cgccaataag
     4801 gtattaagca gaattttaga tttagatccc tttaaaagtg aagaaattgg agaaaataga
     4861 gaattttatg tctctaatat aaatgagttt atagaagaac ctaaaaaata ttctttaagt
     4921 gtagaagatt ctaatgcacc aataaaaatc atggtaatag ctaatagcaa tcttatgaat
     4981 caacttccaa acttaaatag attaaataat agtatagata aagttgagtt taaagtttgt
     5041 tttgatatgt ttatgacata tactgcttca aagtgtgatt tattcattcc ttgtaccaat
     5101 accttagaaa gcgaggatat ggtttttagt tcaatgacta atccatattt aatatataat
     5161 gaaaagataa cagaacctag agaaaaactt atggatgaat actacttttt tagagaatta
     5221 gctaaaagaa tgaacttaaa aggatatcca atcctatcta aaaaagaata tttaagtaag
     5281 gttattgaac ctttaatgag atataataaa tatataacct tagattattt aaaaaataat
     5341 ccttttacag tttatgatga tgtagcttgg gaaaataaaa agtttaaaac accttctggc
     5401 aaatttgaac ttgcatctaa aagagcttta aaagagtgcg gaagcttaac accaacatat
     5461 ataagtccta gaataaaaga aaattgtttt agattgctta ctaatcattc taaagattca
     5521 ttatcaagtc agcattatat agatgtagat gaaaaggcga aagtatattt aaatgagaat
     5581 atgataagaa agttttcctt aatttgcgga gaaaaggtta aattaaaatc aagaactgga
     5641 gaaattacag caatttgttc tttagataat ggagttcaag attatgtggc tttaatgtat
     5701 gttggttggt ggaaaaaaca tgggaatcca aactttttaa ctgaatcagg aatctctgac
     5761 ataggtggac aaataacata taatgaaacc tttatagaga ttgaaaatat ataataaata
     5821 aaaatcaaaa gagaaaggtt gtattttaag agaataaaca ttaaagaaaa aggaggatgc
     5881 ttcataaagt attttttgaa gcgaaaagat atggtgaata aagagattat agaaaatgga
     5941 ttttttaaaa tagaaaaaaa gctaaatgaa gaatttatag gacaaaaaac tttcaataag
     6001 gagttatgtg attactttaa agaaaaaata gaggaagata gtaaaggtat attgcttata
     6061 gtagaggaaa gagacatatt taaaaatagt gtgctaaaat acttttttaa agaacttaat
     6121 tattataagt ttgttaaaaa tagtaaaatt gatgagattg atttagcttc atataagttt
     6181 aatttagggt acaatgcctt cttaacagat ttatatgaaa aattaaataa tgactctcaa
     6241 tgtttagtat ttaaaaatac agataaagca agtgaagata tattaaatgt tctttcagga
     6301 ataagcccta attcttgtat aatgctaaaa gataattatg taataaaaaa taaattttta
     6361 gttgaagctg aagaaattga tgaggatatt gtaaataaaa ttatttgtaa taataaattt
     6421 ttcatattcc tttataacaa ggataaaggt gaagaacttg aaataataga taatgaattc
     6481 ttaataaata aagacaaaac cttatacaga aaagccttaa gcgaaaagga taaaaacaaa
     6541 gtaataaaga aaaagctatt aaaagaaata aataaggtta aggagctttt tgatattgac
     6601 ataataatag gagtagatga aaatccatat gtagaagaaa aatcaggggt ttgtacttat
     6661 atagaagata actttaggga aaaaggaagt ctaagcttag atcaatacat atcatataag
     6721 atttgtaagc ctataatcaa tataattaga aaagaagcta ttgaagcaaa tagtagatta
     6781 cttatatatg tagaagatga tgaaatctat tgtaaatcaa atgatgaggt ttatgattta
     6841 agtaagtata gtaatccaac cttagaagag gttagatata aattagaatc tataattggt
     6901 atgaaagagc ttaaagagtt tatagataag attgaaaata actttaaggt tcaaaaaata
     6961 agagagaaac ttggattaaa gactacacaa atctcattaa atatgatatt tgctggaaat
     7021 gctggtactg gtaaaactaa tgcagcaaga ataacctttg agtatttata tgccttaggt
     7081 atgataaaag aaaatatatt taaagaggtt agtaaagcag attttatagg ggaaggaatt
     7141 agtaatacag taagaagaac taatgaaata atagaatcag ctttaggtgg agtattattt
     7201 atagatgaag cttattcctt atgtacagat aaaaatgata aggtaggtcg tgaaatagta
     7261 gatgctttat taaaaggaat agaagataac agagacaata taaccgttat tttagcaggg
     7321 tatgagaagg atatggaaac tttcctaagt ttcaaccaag gattaaaatc aagatttcca
     7381 aatgtaatta aatttgagga ctataatcct tcagagatgt atgagatagc agttaatatt
     7441 gctaagtcaa agggatatag aatagctaaa agtgcaaagg ctggacttat tgaattattt
     7501 actaaaaacc aaatggttgg gaaaagtgat ttaggaaatg ccagatttgt tagaaatatt
     7561 gtagaaaatg caataatgga tgcatctaag aagtatttag tgaataagga gagagctatt
     7621 gatcttctag aaaaagataa ttttaacttt aaggtaagtg caaaatttga tttagaagaa
     7681 aagttaaaag aaataatagg ccttgaagag gttaaggaat ttttaagaag ccaatataag
     7741 cttatagtag cccaagaaaa gagaaaatct gttggagtaa atacgaaaat agagcaaaat
     7801 ttaaatatga tatttgcagg taaccctgga acaggaaaga ctagtatagc aagattagta
     7861 gctgaaatgt taaatagcat ggggctttta aaggttggac aattagttga aacagataga
     7921 tcaagttttg tttcagatat tccaggagaa acttcaaaga aaactgaaga aaaatttaaa
     7981 gaagccttag gtggagtttt atttattgat gaagcctata ctttagctaa tgattccata
     8041 ggtagagaag ctatagagac acttcttaag ttaatagagg actattcaag agaagtaata
     8101 gttattttag caggatatga agaagaaatg gagaatttct ttgatgttaa cataggatta
     8161 agatcaagat tccctctttg gactaaattt gaagactaca atcctaatga acttttagaa
     8221 atggcaataa aattagtaga agctaaggga tttaaacttt ctaaaaatgg atattcagag
     8281 cttaaaaaga atttcgttga gatatatgaa aatgcagatg ctcaatcagg aaatggaaga
     8341 atggtaagaa actatgttga gcaacttata agaaatcaaa gtattagaat tgcagaaagt
     8401 gatataagtg tttatgaaat gaaccttata aacacaaagg atataaaggc tatgaatgca
     8461 ataaaatatg ataatcactt tgatttagag aaaaagttaa atgatttgat tggaaatgaa
     8521 gagcttaagg attttcttag aggacaatat aaacttatga aaatcagaga aaagagaaag
     8581 aaacttggtt ttcaagttga tttaaataag tatatgaata tggtctttac tggagaaagt
     8641 ggaacaggga aaaagacagt tttaaatata tattcagaaa tgctatatag tatggggata
     8701 atcaaggcaa aaaatatagt tcaaatagat aaatatgaat ttatggcaat gattaattca
     8761 ggtatgaaag ttgaagatat attaaataaa catgttggaa aaatattata tatagataaa
     8821 tggaatactt tctttggaga agaaagatac aatgaaatag tatctgatct tataaaattc
     8881 atagataaca atagcaatag aatagttatt gttcttggtg gaataagagg taagatgaaa
     8941 gatcttattt taactaatga gggattaaat tatagatttc ctgtgtgggt tgactttgga
     9001 aactacaatg aaagagaatt atatgacata gcctcaagca ctttagttaa aaaaggtttt
     9061 accttagatg aagaagctga aactgcttta gaaaatgcta ttggagatat acaaaagaga
     9121 atgggagagc tagcacttaa aaatggtctt atgattaagc aatttttaga tagcttagta
     9181 agagtacaaa gcattagagt ttgtgatgaa gattttgata taaatacaat aaatagaatt
     9241 atatcagagg atatattaaa gagtactgaa atctttttaa agaaaaatac aactcaaaat
     9301 ggaagttttt aaagaattaa atttataaat taaatacttc taagttaggt ataaattcta
     9361 tttgaaaaag tattaaattt acctagattg ctaagatcct agtacaatta atattgtgct
     9421 aggatcttat ttatataaat tacttttttc ttaagataca ctaacgtaat taaattttaa
     9481 tttttactca tatttgccat gatataattt gaaaacagat gattaattaa gcagagtttt
     9541 aactttaaag cttaagtttc tcataataat tattttagga ggtgaggttg ttagtaatag
     9601 gaaaatagtt aaaagtatat tagctattac ttacaacaaa tagagtatga taaagaaaaa
     9661 gagcattatg aaagttcttt gtgggacaat agttagtaca ttcatgtttg gaatggctac
     9721 tccagtattt gcacaaagtg aaaaaactga ggctgtagta actttaggag ctaatttaac
     9781 aaagtcagaa agattacaaa tgttagatgc ttttggagta aaagctaatg aggttaaaat
     9841 aatagatgta actaatcaag atataagaga acaattaggt ttagatacaa gtaaaccaat
     9901 acctgctagc agtcaatcaa tatcaagttc ttacgttgtg gttaaggaca aaggtggaat
     9961 aaatgtaact actaacaatt taacagaggt aacaggaagt atgcttgcta atgcccttct
    10021 tacttcaggg gtaaataatg ctgatgtaaa ggcagatgct ccatttaagg ttacgggaac
    10081 agctgcctta gcaggtattt taaaaggatt tgaagatgca tcaggagagg aattatctct
    10141 tccaaagaaa gaggcggcaa gagaggaaat ttctttaact aataatttaa gtaatgctaa
    10201 aactaaagat ggacaaacac taggaaaaga tgaagccgct gtggtagtaa atgatattaa
    10261 aactgatgta attaaagata aacctaaaaa tgatgaggaa ataggtaaga tagtaaacaa
    10321 tgttacaaat aactataata tacttttaac acaagggcaa caagagcaaa caataaaatt
    10381 tatgtctaaa ataaatgatt tagactataa ctatggtgct atgaaagaat ctttaaatca
    10441 aatgaatgac aagcttcaac agatattaaa agacacagga aaacaattag aagaaagtgg
    10501 tcttttagaa aaagcattaa atggtataaa aaatgtttta gttgatatta aggatttttt
    10561 agtaaatatg tttagctcag ctagtgaaaa agttaaagat ggaataacct acgatgaaaa
    10621 tggtaatata gttataaaaa caggaaataa ttctgatgaa tcaaagaatc aagaaaatat
    10681 ccaagataag ccacaaactc agtcaaatga taataatcaa aatcaacaaa atgaacaagg
    10741 acaaaatcaa acaaaataat tagtattgaa gggcgtgtat caaaataact t
//
LOCUS       DAEVGI010000083        10643 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEVGI010000083 DAEVGI010000000
VERSION     DAEVGI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10643
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.083"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     regulatory      76..181
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            428..1603
                     /locus_tag="K8M77_001685"
     CDS             428..1603
                     /locus_tag="K8M77_001685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBI7086179.1"
                     /translation="MRRLFTSESVTEGHPDKICDQISDAVLDAIFAKDPNARVACETA
                     VTTGLVMVMGEITTNCYVDIPKIARETIKNIGYDRAKYGFDCETCSVMTTIDEQSSDI
                     AMGVDEALESRAGEKIDIDAVGAGDQGMMFGFATNETEEFMPAPIAMAHRLSRRLTEV
                     RKNGTLPYLRPDGKTQVTVEYENDKPVRIDAIVISTQHGPEVSQEQIQADLMEHVIKA
                     VIPAELLDENTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYGRHGGGAFSGKDP
                     TKVDRSAAYAARWVAKNLVAAGIADKLEVQVAYAIGVAKPVSIIVDTFGTGKISDEEI
                     VNIINKVFDLRPGAIIRDLDLRRPIYRQTAAYGHFGRTDLDLPWENLNKVEEIKKYL"
     gene            complement(1740..2873)
                     /gene="nagA"
                     /locus_tag="K8M77_001686"
     CDS             complement(1740..2873)
                     /gene="nagA"
                     /locus_tag="K8M77_001686"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI7086180.1"
                     /translation="MLIKDCNVVYLDKIEKGNILIENGKIKAINPKECNCEIIDGEGL
                     FLSPGFIDVHIHGAGGHDTMDGTYEAINEISKVIVKHGTTSFLPTTMTVAAEDVCKSM
                     EAIHKAKTKGTDGANVLGAHLEGPFISPSAIGAQNPDFLIPPTKENFYKLVGEHEDDV
                     VSITLAPEVEGAKELTKFLSEKGIVVSMGHTKATYEEAMEGIKCGCSHATHLFNAMTP
                     FTHREPGVVGAVFDSEITTETISDGIHIAYPSLRVAYKQKGTDKVLLITDAMMACCMP
                     DGMYSLGGQDVEVKNGAARLLSGSLAGSILTLDVAVKNIFKNTNYSLNEVIKMATYNG
                     AKHCKVDDKKGLIKEDYDADLILFDDNIDIKYVIVNGKLVHKA"
     gene            3010..5244
                     /locus_tag="K8M77_001687"
     CDS             3010..5244
                     /locus_tag="K8M77_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBI7086181.1"
                     /translation="MEVLNGIVESIVFKSSDTGYTVIKFRENNIIHTAVGVLPHVKEG
                     QNLKITGSWVNHSQFGKQFKVEECEEILPTSKDGIEKYLSSGIIQGIGPVTAKKIVNK
                     FGEDTLNILDNNIERLKEIEGIGKKKLETIIESYREQRELKNITIFLQTHGLSVNQCL
                     KIYKKYGASSVDTVKNNPYILCDEISGIGFKTSDKIARSLGIEIDSPFRIQSGIRYVI
                     NEFCTNGHTFMPKDELIKEASNVLTVSEDIIEENIKNAALDRKIKLEKVNDKEGVFTI
                     PNYYCELGITNRILTLAISNFQDISVDVDHLILQFEKKNNITFAESQKDAIISAFQNG
                     IEIITGGPGTGKTTIIKCIIEIFETCGLKVLLGAPTGRAAKRMSESTGKEATTIHRML
                     DMGVFEKEESVFVTNAEEHSLEADVVIIDEASMIDITLMNALLKSIKVGTRLIIVGDV
                     DQLPSVGAGNVLNDFIESGFTKVVRLKEIFRQGKESMIVVNAHKINKGEMPKLNEKGT
                     DFFFIRNDIQEGILNTIIDLINTRLPRFNSNWDKLKSIQVLVPMKKGVLGVTNLNERI
                     QNVLNPKAPYKKEKEFRSMVFREGDKVMQIKNNYSLKWTRIAGKGEHEGLGVFNGDMG
                     FIESIDLEGKKLSIIFDDERRVIYDFMYLDELDLAYAITIHKSQGSEFPVVIIPAYMG
                     APLLMNRNLLYTGITRAKEMVVVVGIPKALKYMVDNTRSMERYSSLNWRIKEVISNEV
                     FEQD"
     gene            5262..6224
                     /locus_tag="K8M77_001688"
     CDS             5262..6224
                     /locus_tag="K8M77_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086182.1"
                     /translation="MSLYGNDLRVENRYSHILKKWYVKSNDRFINLICEPYQKLNFIE
                     SLISDCLINNEKVLYVGKSRKVCKNEQLNSMNFDFANFNNIFNIKKNFDLIIYDDVSL
                     YSNKSSIECNEDLMYLKRLSKKIVICSVDKVFNNIKHIEILNNQRKTHFLEPRIIITR
                     VNLETSIPYTLYDYIEWFIREKRILVVYVPNKFNLNRIYEYYTEDLNLENKVKIVKED
                     KKNSFLNIVSESRFKKEGIIFITDSLHEYFDSIPECDMVIYSFEKDIVDYKKIIFACG
                     ALCKDKFTGREVILLSNEEGDNIETARRLARGFNKTLWKESEIR"
     gene            6200..6859
                     /locus_tag="K8M77_001689"
     CDS             6200..6859
                     /locus_tag="K8M77_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBI7086183.1"
                     /translation="MERVRDKVDYLFQCFLEAIYPTEEKCYVCNTEGEKIICDKCKGK
                     IQKAKETLIIEECKMLICSYYSFIVKDLILRLKYKGDFHAGEILVMLLEEKIKESNLD
                     VDFITYVPVAKDSLRKKEFNQCEYLSKELGKRLGIKSIETLKKRNKVKEQKSLSKEER
                     EKNVQNAFKLKRYKNLEGKSIILLDDVMTTGSTLKACVRELKKIKDIKIFLLTIAKSN
                     I"
     gene            7309..7848
                     /gene="raiA"
                     /locus_tag="K8M77_001690"
     CDS             7309..7848
                     /gene="raiA"
                     /locus_tag="K8M77_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBI7086184.1"
                     /translation="MKVTVHAKNIELTNALKDCVEKKISKLSKYFEPGVEAKATLSVE
                     KNKQIVEVMIPFNGILLRGEESTDDMYKSIDLVEEKIERQIRKQKTRLSKKNRYDSLR
                     FAHIDPIEDMKEEKEAKIVKTKRFDVKPMGAEEAVLQMELIGHDFFVFQDPETNQLNV
                     VYKRKDGNYGLIEPGLDED"
     gene            8081..10603
                     /gene="secA"
                     /locus_tag="K8M77_001691"
     CDS             8081..10603
                     /gene="secA"
                     /locus_tag="K8M77_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBI7086185.1"
                     /translation="MGLFDKIFGSYSDREVKRITPIVDKIDSLGPEMEKLSDEELKQK
                     TFEFKDRYAKGESLDDMLPEAFAVCREASTRVLGMKHYREQLIGGIVLHQGRIAEMKT
                     GEGKTLVATLPVYLNAIAGKGVHVITVNDYLATRDKEWMGQLYEFLGLTTGVIVHGLT
                     NDQRREAYNADITYGTNNEFGFDYLRDNMVIYKEERVQRPLHYCIVDEVDSILIDEAR
                     TPLIISGAGSKSTDLYKIADFFVKKLREEEDYTIDEKAHAAMLTDKGVAEAEKAFGIE
                     NYADANNMELQHHITQALKANYVMKRDKDYMVKDDEIAIVDEFTGRLMEGRRYSDGLH
                     QAIEAKEGVKVQRESKTLATITFQNYFRMYTKLAGMTGTALTEETEFREIYGLDVVVI
                     PTHRPVQRQDHSDLVFKTAKGKYDAIVEEIIETHKTGQPVLVGTTSIEKSEYLSSLLK
                     KKGVPHKVLNARYHEQEAEIVSHAGELGNITIATNMAGRGTDIKLGEGVLEVGGLKII
                     GTERHESRRIDNQLRGRSGRQGDKGHSRFYISLEDDLMRIFGSEKLQAVVDRLGLEET
                     EAIESKMVTKSIENAQKKVEGNNFDIRKTLLGYDDVMNKQREVIYKQRSQVLEGENLE
                     DSVQAMIEDVITNAVQAHLGNIDEDDFEKELGDLIKYLEDIMLPHGKFTVEELKTNSN
                     EEITRKFIECAREIYKEKEEFVGSEQMREIERVIILRVVDTKWMDHIDDMDHLKQGIG
                     LRAYKQQDPIQAYQMEGSAMFDEMINNIKIDTVRYLFHVKVEAEKPQRERVAKETGAS
                     HGGDSQEIKKKPVKKEPKVGRNDLCPCGSGKKYKSCCGREVV"
BASE COUNT         4237 a         1169 c         1976 g         3261 t
ORIGIN      
        1 aaagagacac tattaattaa gtgtctcttt attaaaataa tatcttaata aaatagaata
       61 attagtattt atatactctt atccagagag gtggagggaa aaaggcccta tgaaacccgg
      121 caaccagtgg aaaatcacta cggtgccaat tccggtaaag aaattcttta caagatgaga
      181 gaagataaat ttagtgtata actaaaatct cttcttaaat ctttgaatat ttaagaagag
      241 atttttttat taataaaaat attttaaagg cgcgcattaa aataaatttg ttaattaagc
      301 tttaaagata ttatttgaat cgtgggaaga taaattaagt tattcattta aataaatagg
      361 gttggaataa ataaaaatga aaggggtgaa ttagctatct tattatgata gtaagatagt
      421 attagccatg agaagattat ttacatcaga atcagttact gaagggcatc cagataaaat
      481 atgcgaccaa atttcagatg cagttttaga tgctatattt gcaaaagatc caaacgcaag
      541 agttgcttgt gaaacagctg ttactacagg attagtaatg gtaatgggag aaataacaac
      601 taattgttat gttgatatcc ctaaaatagc tagagaaact ataaagaata taggatatga
      661 tagagctaaa tacggtttcg actgtgaaac ttgttcagtt atgactacaa tagatgagca
      721 atcatcagat atcgctatgg gtgttgatga agctttagaa tcaagagctg gagaaaaaat
      781 tgacatagat gcagttggag caggggacca aggaatgatg tttggttttg ctactaatga
      841 aactgaagaa tttatgccag caccaatagc tatggctcac agattatcaa gaagattaac
      901 agaagtaaga aagaacggaa ctttacctta cttaagacca gacggaaaaa cacaagttac
      961 tgttgaatat gaaaatgaca aaccagttag aatagatgca atagtaattt caacacaaca
     1021 tggtccagaa gtatctcaag aacaaataca agctgattta atggaacatg ttataaaagc
     1081 tgttatacca gcagagttat tagatgaaaa tactaagtat tatataaatc caacaggaag
     1141 attcgttatc ggtggacctc aaggagatgc tggattaaca ggaagaaaga tcatagttga
     1201 tacttacggt ggatatggaa gacacggcgg tggtgctttc tcaggaaaag atccaactaa
     1261 agttgataga tcagctgcat acgctgctag atgggtagct aaaaacttag ttgctgcagg
     1321 aatcgcagat aaattagaag ttcaagttgc ttatgctata ggagttgcta aaccagtatc
     1381 aattatagta gatacttttg gaactggaaa aatatcagac gaagaaatag ttaatataat
     1441 aaacaaagtg tttgatttaa gaccaggagc tataataaga gatttagatt taagaagacc
     1501 tatatataga caaacagctg cttatggaca ctttggaaga acagatttag atcttccatg
     1561 ggaaaactta aacaaagttg aagaaataaa gaaatatctt taatatatag aaaagcttag
     1621 gattttatat cctaagcttt ttatttttat ataaatttat tattttaaga tgaactagta
     1681 attttaaaat taagataaaa taaaaaagca ctttgaaaaa tcaaagtact ttttttatat
     1741 taagctttat gaactaattt tccattaact attacatatt ttatatctat attatcatca
     1801 aaaagaatta agtcagcatc atagtcttct ttaattaatc cttttttgtc atcaactttg
     1861 cagtgtttag ctccgttata agtagccatt tttataactt cattaagtga ataattagtg
     1921 tttttaaata tatttttaac tgccacatct aaagttaaaa ttgatcctgc taaagaacca
     1981 ctcaataatc ttgcagctcc atttttaact tcaacatctt gtcctcctaa tgaatacatt
     2041 ccatcaggca tacagcaagc catcatagca tcagttatta ataaaacctt atctgttcct
     2101 ttttgtttat aggcaactct taaacttgga tatgctatgt gaataccatc tgagattgtt
     2161 tcagtagtta tttcactatc aaaaacagca cctacaactc caggttctct atgagtaaat
     2221 ggagtcattg cattgaataa atgagtggca tgagagcatc cacattttat tccttccata
     2281 gcttcttcat aagtagcttt agtatgtccc attgaaacaa ctataccttt ttcacttaag
     2341 aatttagtaa gttctttagc gccttctact tcaggagcaa gagttattga aacaacatca
     2401 tcttcatgtt ctccaactaa tttatagaag ttttcttttg ttggagggat taagaaatca
     2461 ggattttgag cacctattgc acttgggctt ataaatggtc cttctaagtg agcacctaaa
     2521 acattagctc catctgttcc tttagttttt gctttatgaa tagcttccat tgatttgcat
     2581 acgtcttcag cagctacagt cattgttgtt ggtaaaaatg aagtagtacc atgtttaact
     2641 ataacttttg atatttcatt tatagcttca taagtaccat ccatagtatc gtgaccacca
     2701 gcaccgtgta tatgaacatc tataaatcct ggagatagga ataagccctc tccatcaatt
     2761 atttcacaat tacattcttt aggatttata gcctttatct ttccgttttc tataagtata
     2821 tttccttttt caatcttatc taaatatact acattacagt cttttattaa catacgaatc
     2881 accttcctca taataatctt aaaattttaa tttatgtaag tcaataaaat aagcactatg
     2941 atttaaaatt gataattgtg atataattaa tagtaataac aatttaagag aaacctttta
     3001 ggaggtaata tggaagtatt aaatggaata gttgagagta tagtatttaa aagttcggat
     3061 acagggtata cagttataaa atttagagaa aataatatta ttcatacagc agtgggagtt
     3121 ttaccacatg taaaagaagg tcaaaattta aaaattaccg gaagttgggt taaccattca
     3181 cagtttggaa aacaatttaa agtagaagaa tgtgaagaga tattacctac ttcaaaggat
     3241 ggaatagaaa aatatttatc ttctgggata atacagggga taggacctgt tacggcaaaa
     3301 aaaattgtaa ataaatttgg agaagatact cttaatattt tagataataa tattgagaga
     3361 ttaaaagaaa tagaaggaat aggtaaaaag aagttagaga ccataattga gtcttataga
     3421 gaacaaagag aattaaaaaa tataactata tttttacaaa cccatggatt aagtgtaaat
     3481 cagtgcttga agatttataa aaaatatgga gctagttcag ttgatacagt taaaaataat
     3541 ccatatatac tttgtgatga aatttcagga ataggattta agacatctga caaaattgct
     3601 aggagtttag ggatagaaat tgattcacct tttagaattc aaagtggtat aagatatgta
     3661 ataaatgaat tttgtactaa tgggcacaca tttatgccaa aggatgaact tataaaggaa
     3721 gcaagtaatg tattaactgt aagtgaagat ataatagaag aaaatattaa aaatgcagct
     3781 ttagatagaa aaataaagtt agagaaagtt aatgataaag aaggtgtttt cacaatacct
     3841 aattattatt gtgagcttgg aataactaat aggattttaa ctttagctat aagcaatttc
     3901 caagatataa gtgtagatgt agatcaccta atacttcaat ttgaaaagaa aaataatata
     3961 acttttgcag aatcccagaa agatgctata ataagtgctt ttcaaaatgg aatagagata
     4021 ataactgggg gaccaggaac aggaaagacc actataataa agtgtataat tgaaattttt
     4081 gaaacctgtg gtttgaaggt tcttttggga gcacctactg gaagagcggc taaaagaatg
     4141 agtgagtcta caggaaaaga agctaccaca atacatagaa tgcttgacat gggagtcttt
     4201 gaaaaagaag aaagtgtttt tgtaaccaat gcagaggaac attccttaga ggctgatgtg
     4261 gttataatag atgaggcatc aatgattgat ataactttaa tgaatgcttt acttaaatct
     4321 attaaggtag gtactaggct tataatagta ggagatgttg atcagcttcc atcagtagga
     4381 gctggaaatg tattaaatga ttttatagag agtggattta caaaggttgt acgtctaaag
     4441 gaaatattta gacaaggaaa agagagtatg attgtagtaa atgcacataa aataaataaa
     4501 ggtgagatgc ctaagttaaa tgaaaaaggt actgatttct tcttcataag aaatgatatt
     4561 caagaaggaa tattaaatac tattatagat ttaataaata ctaggttgcc taggtttaat
     4621 tcaaattggg ataagttaaa aagtatacag gttttagttc ctatgaaaaa gggtgtatta
     4681 ggagttacaa atttaaatga aagaattcaa aatgtcttaa acccaaaggc tccatataaa
     4741 aaagaaaaag aatttagaag tatggtattt agagagggag ataaggttat gcagataaag
     4801 aataactact ctctaaaatg gaccagaata gctgggaagg gtgaacatga aggcttgggt
     4861 gtatttaatg gagatatggg atttatagaa agtatagatt tagaagggaa aaaattatct
     4921 ataatctttg atgatgaaag aagagttatt tatgatttta tgtatttaga tgagttagat
     4981 ttggcttatg caatcacaat acataaaagt caaggaagtg aattccctgt agtaataata
     5041 cctgcatata tgggagcacc attacttatg aatagaaatt tattatatac aggaataact
     5101 agggcaaaag aaatggttgt agtagttgga atacctaaag cattaaaata catggttgat
     5161 aatacaagga gtatggaacg ttattcctct ttaaattgga gaataaaaga agtaatatct
     5221 aatgaggttt ttgaacaaga ttaaattagg gggatttaga agtgagctta tatggcaatg
     5281 atttaagggt tgaaaataga tatagtcata tattaaagaa atggtacgta aaaagcaatg
     5341 ataggtttat aaatttaata tgtgagccct atcaaaagtt gaactttatt gaaagtttaa
     5401 ttagtgattg tttaataaat aatgaaaaag ttttgtatgt aggtaagagt agaaaagttt
     5461 gtaagaatga gcaattaaat tctatgaatt ttgattttgc aaattttaat aatattttta
     5521 atataaaaaa gaattttgat ttaataatat atgatgatgt aagcctatat tcaaataaaa
     5581 gtagtattga atgcaacgag gatttaatgt acttaaaacg cctatctaag aaaatagtaa
     5641 tatgttcggt agataaggta tttaataata taaagcacat agaaatatta aataatcaaa
     5701 gaaaaacaca tttcttagag ccaagaataa taataactag agttaattta gaaacttcaa
     5761 taccttatac cttatatgat tatattgaat ggtttataag agaaaagaga atcttggttg
     5821 tatatgttcc aaataaattt aacttaaata gaatttatga atattatact gaggatttaa
     5881 atttagaaaa taaggttaaa atagtaaaag aagataaaaa gaattccttt ttaaatatag
     5941 taagtgaaag tagatttaaa aaagaaggta ttatttttat aacagattca ttacatgaat
     6001 attttgattc tattccagaa tgtgatatgg tgatatattc ttttgaaaaa gatattgttg
     6061 attacaaaaa aataatattt gcctgtggag ctttatgtaa agataaattc actggaagag
     6121 aagtaatatt attaagtaat gaagagggag ataatataga aactgctagg aggttagcaa
     6181 gaggctttaa taaaacttta tggaaagagt cagagataag gtagattatt tatttcaatg
     6241 ttttttagaa gctatttatc caacagaaga aaagtgttat gtatgcaata cagaaggaga
     6301 aaaaataatt tgtgataaat gcaaaggaaa aatacaaaag gcaaaggaaa ctttgattat
     6361 tgaagaatgt aaaatgttaa tctgttctta ttattctttt atagttaagg atttaatatt
     6421 aagacttaaa tataaaggag attttcatgc tggtgaaata ttagttatgt tactagaaga
     6481 aaaaataaaa gaatcaaatt tagatgttga ttttataaca tatgttcccg tagctaagga
     6541 ttctcttaga aagaaggaat ttaaccaatg tgaatattta tcaaaagaat taggaaagcg
     6601 actagggatt aaatccatag aaactttaaa aaagagaaat aaggtgaaag aacaaaagtc
     6661 attatctaaa gaagaaagag aaaaaaatgt tcaaaatgct tttaagctta aaagatataa
     6721 gaatttagaa ggaaaaagta ttatactttt agatgatgta atgactacag gttctacatt
     6781 aaaggcctgt gtaagagaac taaaaaaaat taaagatatt aaaatttttt tattgactat
     6841 tgctaaaagt aatatataat aataatgtag ctaggtttgt ggttgaaagt cgatgccagt
     6901 cgcaggcgaa acgatccacg taagttaaac aaagttttaa tgagcatggt gcggcttaga
     6961 agtaagacct accagccaat aaaggctgag agggttagta gtgagaggtt aactccgggt
     7021 agcgaaagct ccagtaggcg agtgtggggt caaagaccag gtcaactagc tataaaaagg
     7081 gactatgatt ttcatggtcc ttttttattt tttgtcaagt tacatctctt ataaataaat
     7141 taagtaaaga gaagatttaa ttagacaatt aagatttata ttaaaaaaat taagttaatt
     7201 aaaataatta aaacttgtat aaaatctttt aaaagagtaa aattataata taatatttaa
     7261 acctttacat tgattgaggt tacttgtttt ttgaaagggg ctgacgatat gaaagtaaca
     7321 gtacatgcta aaaatataga gttaacaaat gctttaaagg attgtgtaga aaagaaaata
     7381 tccaaattaa gtaagtattt tgaaccagga gttgaagcaa aagccacttt aagcgtagag
     7441 aaaaataaac aaatcgtaga agttatgatt ccttttaatg gaatattatt aagaggtgaa
     7501 gaatctacag acgacatgta taagtcaata gatttagttg aagaaaaaat cgaaagacaa
     7561 ataagaaaac aaaaaactag attatcaaag aaaaatagat atgattcatt aagatttgct
     7621 catatagatc caatagagga tatgaaagaa gaaaaggaag caaaaatagt aaaaactaaa
     7681 agatttgatg ttaagccaat gggagctgaa gaagcagttt tacaaatgga gcttatagga
     7741 catgacttct ttgtattcca agatccagag acaaatcaat taaatgtagt ttataaaaga
     7801 aaagatggta attatggttt aattgaacca ggattagatg aagattaata gaataaatgt
     7861 aaaaaagatg caagttttta cttgcatctt ttatttttat gtaaaagttt tatctaaatt
     7921 attacattta ttgaaaatag gttactataa atggtataat ctaatgagaa ttattttaaa
     7981 taattttttt ataaaattta gaataatgta tatatgtgtt ataatgagat aattattaga
     8041 atattaagca tataagaaag tagaattgag ggatattata atgggattat ttgataaaat
     8101 atttggaagt tatagcgata gagaagttaa gagaataaca cctattgtag acaaaataga
     8161 ttcacttggg cctgaaatgg aaaaactatc tgatgaagaa ttgaagcaaa aaacatttga
     8221 atttaaggat agatatgcta aaggagaaag cttagatgat atgcttccag aagcttttgc
     8281 tgtttgtaga gaagcatcaa caagagtttt aggaatgaaa cactatagag agcaattaat
     8341 tggtggtata gttttacacc aaggaagaat agcagaaatg aaaactggtg aaggtaaaac
     8401 attagttgct actttacctg tttatttaaa tgctatagct ggtaaaggtg ttcatgtaat
     8461 tacagttaat gattatcttg caacaagaga taaagagtgg atggggcaat tatatgaatt
     8521 cttaggatta actactgggg ttatagttca tggattaact aatgaccaaa gaagagaagc
     8581 atataatgcg gatataactt atggaacaaa taatgaattt ggatttgatt atttaagaga
     8641 taacatggtt atttataaag aagaaagagt tcaaagaccg ttacactact gtatagtgga
     8701 tgaggttgac tcaattctta tagatgaagc aagaactcct cttataattt caggtgcagg
     8761 aagtaaatca acagatcttt ataagatagc tgatttcttt gttaagaaac taagagaaga
     8821 agaagattat acaatagatg aaaaagcaca tgctgcaatg cttactgaca aaggagttgc
     8881 agaggcagaa aaggcttttg gaatagaaaa ctacgcagat gctaataata tggaattaca
     8941 acatcatatt actcaagcct taaaagctaa ctatgtaatg aaaagagata aagattacat
     9001 ggttaaagat gatgaaatag ctatagttga tgaatttaca ggaagactta tggaaggtag
     9061 aagatattca gatggtcttc accaagccat tgaagctaag gaaggtgtta aagttcaaag
     9121 agaatctaag actttagcaa ctataacatt ccaaaactat ttcagaatgt atactaaatt
     9181 agcaggtatg acaggtactg cgcttactga ggaaactgaa tttagagaaa tatatggatt
     9241 agacgttgtt gttattccaa ctcatagacc agttcaaaga caagaccact cagatttagt
     9301 atttaaaaca gctaagggaa aatatgatgc catagtggaa gaaataatag aaactcataa
     9361 gacaggtcaa ccagtattag ttggtacaac tagtatagaa aaatcagaat atctttcatc
     9421 tttattaaag aaaaaaggtg ttcctcataa ggttttaaat gctaggtatc atgaacaaga
     9481 agctgaaatt gtatctcatg caggtgagtt aggtaacata acaattgcaa ctaacatggc
     9541 tggtagaggt actgatatta aacttggaga aggtgtttta gaagttggtg gtttaaaaat
     9601 cataggtact gaaagacatg agtcaagaag aatagataat cagcttagag gacgttcagg
     9661 tcgtcaagga gataaagggc attcaagatt ctatatctct ttagaggatg atttaatgag
     9721 aatatttggc tcagaaaaac ttcaagctgt tgttgataga ttaggattag aagaaactga
     9781 ggctattgaa agtaaaatgg ttactaaatc aatagaaaat gctcaaaaga aagtagaagg
     9841 taataacttt gatataagaa aaacattgtt aggatatgat gatgttatga ataaacaaag
     9901 ggaagttatc tataagcaaa gatcacaagt tttagagggt gaaaacttag aagacagcgt
     9961 tcaagctatg atcgaagatg ttataactaa tgctgttcaa gctcacttag gaaatattga
    10021 tgaagatgat tttgaaaaag aacttggaga tttaataaaa tacttagaag atataatgtt
    10081 accacatggt aaattcactg tagaagaatt aaaaactaat tcaaatgaag aaattactag
    10141 aaaattcatt gaatgtgcta gagaaatcta caaggaaaag gaagagttcg ttggctcaga
    10201 gcaaatgaga gaaatagaaa gagttataat cttaagagtt gttgacacta agtggatgga
    10261 tcacatagat gatatggatc acttaaaaca aggtattgga ttaagagcat acaaacaaca
    10321 agatccaatt caagcttacc aaatggaagg tagtgctatg tttgatgaaa tgataaataa
    10381 tataaagata gacacagtaa gatacttatt ccatgtaaaa gttgaggctg aaaaacctca
    10441 aagagagaga gttgctaagg aaacaggagc aagtcatggt ggagactctc aagaaataaa
    10501 gaaaaaacca gttaaaaaag agcctaaagt aggtagaaat gatttatgtc catgtggaag
    10561 tggaaaaaaa tataaatcat gttgtggaag agaagtagtt taatataatt aaaagccttt
    10621 gcattatttg cagaggcttt taa
//
LOCUS       DAEVGI010000084        10517 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEVGI010000084 DAEVGI010000000
VERSION     DAEVGI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.084"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            126..788
                     /locus_tag="K8M77_001692"
     CDS             126..788
                     /locus_tag="K8M77_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HBI7086186.1"
                     /translation="MWGKIRNVLGILLIVAGVSLIGVTIWMKYDTYRQQQAVLDSFRN
                     LQFDVPEGEKKDTDVLEENTKENSDEKNKADKVEVEKDKKPEKAQLEEGKGIGILNIP
                     KINLEIGIIEGVSYEDIKYVVGHFPGSPMPGEKGNFSIAGHRISYFGQAFKDIDKLEK
                     GDKVKVTYNGKEYTYEITDMYEVTPNETEALNPTKDATITIVTCTTDAKNRVIVKGKL
                     VE"
     gene            849..1862
                     /locus_tag="K8M77_001693"
     CDS             849..1862
                     /locus_tag="K8M77_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HBI7086187.1"
                     /translation="MSEVTFRKDKYMFSTRNIKKNLDKNILKKEISTFIKELRKFKVD
                     LKSLSSEEFNLEERNLILNIAYFLVDEEVLLRKIINRRELKTKVIAKKTGFSNEKIIS
                     WSNYLIAYLILLYNADYTNLAMYLNINFKDLENLAVIKGTKGEEIVHKGLVMLEGKKS
                     TIILTKEGQFVRIKTRCENKVGEELSGKEKKTLKHYRALIFSVALIAFVLIGSVTFLY
                     KQQETTILIQGTSKVKIGLNRFDRIVYSYSPTEKGTILITELKLENKKFEDGMISILK
                     GFEEEDMLPPNRIVDVYITGKMVDTVELNKVTEYVESVNKDDNAKNNFRIKINNSGYE
                     KKN"
     gene            1884..2780
                     /gene="rluF"
                     /locus_tag="K8M77_001694"
     CDS             1884..2780
                     /gene="rluF"
                     /locus_tag="K8M77_001694"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBI7086188.1"
                     /translation="MRKVSKNNNRRDKSLFNKKFKSEDKNLNKANKNNKQKVAKKASV
                     KNTIIVSNKNEIRDEVRLNKYISETGFCSRREADKLIEQGRVKIDGVKATTGMKVSKG
                     QSVFIDGKPLKVENELVYIVLNKPVGITCTTESKIKGNIVDFINHEKRIFPIGRLDKD
                     SQGLIFMTNDGDIVNKILRAGNNHEKEYIVTVNKPITDEFIKGMSNGVPILGTVTKKC
                     LVKKESKNSFRIILTQGLNRQIRRMCEYFGYEVKKLERIRIMNVSLGNLKIGSWRYLT
                     KKELAEINRLTENSSKTEEASI"
     gene            complement(3022..3963)
                     /locus_tag="K8M77_001695"
     CDS             complement(3022..3963)
                     /locus_tag="K8M77_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7086189.1"
                     /translation="MTYIIILIIIAILIIVFFLATGLYIFKSTVTRKLHDIEKSYTRY
                     VENNLFDEALYNSASKEDITLKSFDGLNLTSTLIMNENPTNKFIILVHGVSICYVGSL
                     KYFDIFYKNGFNVLIVNQRRHGKSEGKYSTYGFYEKYDVNMWIEYLKSRFGNDIILGL
                     HGESMGAGTVMETIPLNDSIKFVIEDCGYSNFHELIGFQITHAYKNRFVRKILRPSLI
                     FANLFMKTKAKFSMKKIVPIDIVSSTSLPMMFIHGKEDYFVPWYMSLDLYNAKTKGYK
                     KLYLVEGAKHAEALEVNKILYEKKIMTFIEKALSLYK"
     gene            4074..4784
                     /locus_tag="K8M77_001696"
     CDS             4074..4784
                     /locus_tag="K8M77_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBI7086190.1"
                     /translation="MKFKKVFLCLMSLLLIFSTVVLGEEINKIEVIEKSIEENTDKYE
                     IKVKYPIIQGGEKEITDKINKTIEDYTLSWINDIKLLGKEYSKEYEKAGKEMPKMEAY
                     SLFEAFNTDEVISLPVSYYQYTGGAHGLTTKVSYNYNLKTGKELKLKDLFKEGFDYKC
                     IIDKKVKEDIEKEKELYFDNGALFKGVNENQAYYLNRDGIIVYFQQYEIAPYSSGIRE
                     FKIPYGEFEEGLIYKLGN"
     gene            4936..6312
                     /locus_tag="K8M77_001697"
     CDS             4936..6312
                     /locus_tag="K8M77_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBI7086191.1"
                     /translation="METLREKINKQRKYFSTGETKDINFRIEKLKKLRDVLKSEEEKI
                     FEALKKDLMKSSFESYVTEVAMVYDEINMHIKNIKKWSKKRRVKTPLVQFPAKSFIKL
                     EPYGVVLIIGPFNYPFMLTMDPLIGAIAAGNTAVIKPSESAPETSKILKEILEKVFDE
                     KYVLHVNPERGKEVVEELLKEKFDYIFFTGSATVGKIVMKAASQYLTPITLELGGKSP
                     CIIDKDCKLELAARRIVWGKLLNSGQTCVAPDYLYVHKDIEEEFIKKLEEEIKNQFGN
                     NPLESEDYSKMVNEREFNRVLSYIDKEKLVFGGNYNRKTFQIEPTILKNVTWNDPVME
                     REIFGPIFPILSFENLDEVIRLVNSKDKPLALYYFSEDKNKIEKVINSTSSGGVTIND
                     TLVHVSSSYLPFGGVGNSGMGEYHGKYSFDLFSNKKGVMNRKTFLDLKIRYAPFLNKL
                     TIVKKIMK"
     gene            7008..8387
                     /locus_tag="K8M77_001698"
     CDS             7008..8387
                     /locus_tag="K8M77_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBI7086192.1"
                     /translation="MKYTFPENFWWGAATSGPQSEGRFNKKHDNVFDHWFDINPELFH
                     NGIGPNIASNFYNSYKEDLAMLKEIGLNSFRTSIQWTRVIKDFETGDIDEDGVRFYNG
                     VIDECLANEIDIIMNLHHFDLPVELYDKYGGWESKHVVELFAKFAKTAFSLFGDRVKK
                     WATFNEPIVIIEGQFLYKWHYPCIVDGKRGLQAAYNIALASARAIEEYRKLGQDGEIG
                     IIVNLTPAYPRSESKEDLRAAEIANAFFNELFLDPATKGEFPKNLVEVLEKDGVMWNS
                     TKEELQVIKNNTVDFLGVNYYQPRRVKAREEEYGGETWAPEKYFDNYDMPGKRMNPHR
                     GWEIYPKAIYDIAKNVQENYGNIKWFISENGMGVEGEEKFKNAEGIIEDDYRIEFIIE
                     HLEWLHKAIEEGSNCVGYHLWTPIDCWSWLNSYKNRYGFISLDLETQKKTIKKSGRWI
                     KEVSKNNGF"
     gene            8624..9694
                     /locus_tag="K8M77_001699"
     CDS             8624..9694
                     /locus_tag="K8M77_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBI7086193.1"
                     /translation="MLYSLILAGGKGTRLYPLSRSSNPKQFLSIHNNGKSFLSNTVDR
                     IRPIIHKDNIYVVTNQEYEDKIRDELKDIREENIFTEPANKETATCIGLSAAKLLKKD
                     KDAVMIVLPSDHYIEGDKSYLQTLEQAVEIADKKRAIVTIGIEPTRPETGYGYIEMGD
                     RVNGHIPTFKIARFTEKPNLEVAKDFLLKGTYLWNSGMFIFRADVILREIEKYIPKMY
                     KCLMEIYQHIGEDDEEEIIKEQYSLIDGISIDFGVMQKTRRSFVIKCDFSWDDIGTFS
                     ALSRFLGPNGNNRTSDNVFLEECENCSVFGKEKLIIGFGIKDLVLVDAGDVILVMDKG
                     RDQEIKHLLNSISGKKEFKEYL"
     gene            9923..10489
                     /locus_tag="K8M77_001700"
     CDS             9923..10489
                     /locus_tag="K8M77_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBI7086194.1"
                     /translation="MSDRIVDFNELKNKARDKDIDKFESYIYDLYYSVAQGKMSMMEL
                     SREITKYMNENNISQEKFLNIQTEMLKRYGFDMGDIEGQMKNLGIDMSNLGIQGGDYE
                     NLRKTLSFQEKYKGKIKTSTVTTYFIKNEKNDIELILENNKVLITSPKNIDLQDSELN
                     EFLCSYKKTLSDDLLDIRICENIGQYNY"
BASE COUNT         4277 a          995 c         1850 g         3395 t
ORIGIN      
        1 taataaattg agatttaatt aaagagaata gcttataata atttattaga aataatttta
       61 gtatttagta atattattag aagctttatg taaaagggat ttaaatagaa agggtgaatt
      121 gagaagtgtg gggaaaaatt agaaatgtat taggtattct tttaatagtg gcaggagtat
      181 cgcttattgg agtaaccatt tggatgaaat atgatactta tagacagcag caagctgttt
      241 tagatagctt tagaaattta cagtttgatg ttccagaggg tgaaaagaaa gatactgatg
      301 tgttagaaga aaatactaaa gaaaatagcg atgaaaagaa taaagctgat aaggttgaag
      361 ttgaaaaaga caaaaagcca gaaaaagcac aacttgaaga gggaaaagga attggtattt
      421 taaatattcc aaagataaac ttagaaatag gtataataga aggcgttagc tatgaagata
      481 taaagtatgt agtaggtcat tttcctggtt caccaatgcc aggggaaaaa ggtaatttct
      541 ccatagcagg acatagaata tcatattttg gacaagcttt taaggatatt gataagcttg
      601 aaaaagggga taaagtcaaa gttacttaca atggaaaaga atatacttat gaaataactg
      661 atatgtatga ggttactcca aatgaaactg aggctttaaa tccaacaaaa gatgctacta
      721 taactatagt tacatgtaca actgacgcta aaaatagggt tatagtaaaa ggaaagttag
      781 tggaataaga ctgggttttt gccttagtct tattttttat aaaatataca taaaggagtg
      841 tcgcttgagt gagtgaagta acttttagaa aagataaata tatgttttct actagaaata
      901 taaagaaaaa tctagataaa aacatattga aaaaagaaat aagtactttc ataaaagagc
      961 ttagaaaatt taaagttgat ttaaaaagtc tttcaagtga agagtttaat cttgaggaaa
     1021 gaaatttaat tcttaatata gcctatttcc tagtggatga agaagtatta ttaagaaaga
     1081 taataaatag aagagagtta aaaacaaagg ttatagctaa aaaaacagga tttagtaatg
     1141 aaaagattat aagttggtca aattatttaa tagcgtattt aattttatta tataatgcag
     1201 attatacaaa tttggctatg tatttaaata taaattttaa ggacttagaa aacctagctg
     1261 ttataaaggg gacaaaaggg gaagagattg ttcataaagg tctagttatg ttagaaggta
     1321 aaaagagtac cataatatta actaaagaag gccaatttgt aagaataaaa acaagatgtg
     1381 aaaataaagt tggagaagaa ttgtcaggaa aagagaagaa aactttaaag cattatagag
     1441 ccttaatatt tagcgtggct ttaattgcat ttgttcttat aggaagcgtt acttttcttt
     1501 ataaacaaca ggaaactacc atattaattc aaggaacatc aaaggttaaa attggattaa
     1561 ataggtttga tagaatagtt tatagttatt ctccaacaga aaagggaact atattaataa
     1621 cagaattaaa attagaaaac aagaaatttg aagatggtat gatttcaatt ttaaaaggtt
     1681 ttgaagaaga ggatatgctt cctcctaata gaattgtgga tgtttatata actggaaaga
     1741 tggttgatac tgtagagtta aataaggtta cagaatatgt tgagagtgta aataaggatg
     1801 ataatgcaaa gaataatttt agaataaaaa taaataattc aggctatgag aagaaaaatt
     1861 aattaaggaa aggattattt atagtgagaa aagtatcaaa aaataacaat agaagagata
     1921 agagtttatt taataaaaag tttaagagtg aagataaaaa tttaaataaa gctaataaaa
     1981 ataataaaca aaaagtagct aaaaaagctt cagtaaaaaa tactattata gtatcaaata
     2041 aaaatgaaat aagagatgaa gttagattaa ataaatatat aagtgaaaca ggattttgct
     2101 caaggagaga ggctgataag cttatagaac aaggaagagt taagatagac ggggtaaagg
     2161 ctacaactgg tatgaaggtt tcaaaggggc aaagtgtatt tattgatgga aaacctctaa
     2221 aggttgaaaa tgaactagtt tatatagttt taaataagcc tgttggaata acttgtacta
     2281 ctgagagcaa gataaaaggt aatatagttg actttataaa tcatgaaaaa agaatttttc
     2341 caataggaag attagataag gattctcaag gattaatatt tatgactaat gatggtgata
     2401 tagttaataa gatattaaga gctgggaata atcatgaaaa agaatatatt gtcactgtaa
     2461 ataagcctat aactgatgag tttataaaag gaatgagcaa tggagtgcct atactaggaa
     2521 cagttactaa gaaatgctta gttaagaaag aaagcaaaaa ttcatttaga ataatattaa
     2581 ctcaagggtt aaatagacag ataagaagaa tgtgtgaata ttttggatat gaggtcaaaa
     2641 aattagaaag aataagaata atgaatgtta gcttaggaaa cctaaagata ggatcttgga
     2701 gatacttaac taagaaagag ttagctgaaa taaacaggtt aacagaaaac tcttcaaaga
     2761 ctgaagaggc ctcaatttaa ttgaatgtta attactagat atatattcag gtctaactta
     2821 atttcgcaaa gagtttctat tgtttattcc agaataatag ctaaggcttt taaactcgct
     2881 gtgctcaaac agtaaaagcc tcttaacgct attattctta caaaaacaat gaaactctaa
     2941 tagctcatta agatggcctt tcatctatat ctagtaatta atacagttca aaataattta
     3001 aatagaattt gtttttataa tttacttata caaagataat gctttttcga taaaggtcat
     3061 tatctttttt tcatatagaa ttttattaac ttctaaggct tcagcatgtt tggcaccttc
     3121 aactaagtag agttttttat agcctttagt tttagcgttg tataaatcta atgacatata
     3181 ccatgggaca aagtaatctt cttttccatg tataaacatc attggtaatg aggttgaaga
     3241 tactatatct ataggtacta tttttttcat agaaaattta gctttagttt tcataaataa
     3301 gtttgcaaat ataaggcttg gacgaagaat ttttctaaca aatctatttt tatatgcatg
     3361 tgttatttga aatcctataa gttcgtgaaa gttagagtaa ccacaatctt caattacaaa
     3421 ttttatggaa tcatttagag gaatagtctc cataactgtt ccagctccca ttgattcacc
     3481 atgaagacct aaaattatat cattgccaaa tctagatttt aagtattcta tccacatgtt
     3541 tacatcatat ttttcataaa aaccatatgt ggaatatttt ccttcacttt ttccatgtct
     3601 tctttgatta actataagaa cattaaatcc atttttatag aatatatcaa agtattttaa
     3661 agaaccaaca taacaaatag aaactccatg gactaatatt ataaatttat ttgtaggatt
     3721 ttcattcatt ataagtgttg aggttaaatt aagaccatca aaggatttta aagtaatatc
     3781 ttccttagaa gcagaattat ataaagcttc gtcaaaaaga ttattttcca catatctagt
     3841 ataacttttc tctatatcgt gaagttttct agttacagta gatttaaata tatatagacc
     3901 tgttgctaaa aaaaagacaa taattaaaat agcaataatt attaatataa taatataggt
     3961 cacttatatc actccaatca gtttttaata tattttattt atacattata cataattata
     4021 acacttaaga gaaaaataaa attagttaaa aatataaagt ggaggtttac tttatgaaat
     4081 ttaaaaaagt ttttttatgc cttatgtcat tacttcttat tttttctacc gtagttttag
     4141 gagaagaaat aaataaaata gaagttatag aaaaatctat agaagaaaat actgacaagt
     4201 atgaaattaa agttaaatac ccaataattc aaggtggaga aaaagagatt acagataaaa
     4261 taaataaaac tatagaggat tatactttaa gttggataaa tgacattaag ttattaggaa
     4321 aagagtacag caaagaatat gaaaaggcag gaaaagaaat gcctaaaatg gaggcatata
     4381 gtctttttga agcatttaat acagatgaag taattagttt acctgtttca tattatcaat
     4441 atactggtgg agctcatgga ttaacgacta aagtaagtta taattataat cttaaaacag
     4501 gaaaagagct taaattaaaa gacttattta aagaagggtt tgactataaa tgtataattg
     4561 ataaaaaggt aaaagaggat attgaaaaag agaaggagct ttattttgat aatggagctt
     4621 tatttaaagg agttaatgaa aatcaagctt attatttaaa tagagatgga attatagttt
     4681 attttcagca gtatgaaata gcaccatact cttccggtat tagagaattt aaaattcctt
     4741 atggagaatt tgaggaaggg ttaatttata aattaggaaa ttaatattaa gagcgtgtat
     4801 caaagtatat ttattattaa attttgatac acgttttttg ttgttaaatt taaaagtata
     4861 tattttatta ttaaataaaa tagagatata ataaattaaa aaaaggaaat ggaataatga
     4921 gggagtggaa attctatgga aaccttaaga gaaaaaataa ataaacagag aaaatatttt
     4981 agtacaggtg aaactaagga tataaatttt agaatagaga aactaaagaa gttaagagat
     5041 gttttgaaaa gtgaggaaga gaaaattttt gaagctttaa aaaaagattt aatgaaatct
     5101 agctttgaat cctatgttac agaagtggca atggtatatg atgaaataaa tatgcacatt
     5161 aaaaatataa aaaaatggag caaaaaaaga agagtaaaaa ctccattagt acaattccca
     5221 gcaaaaagtt ttataaagtt agaaccttat ggagttgttt taataatagg cccttttaat
     5281 tatcctttta tgcttacaat ggatccttta attggagcaa tagcagctgg aaacacagca
     5341 gttataaagc cttctgaatc agcaccagag acatctaaaa ttcttaaaga aatattagaa
     5401 aaagtatttg atgaaaagta tgttcttcat gttaatccag aaaggggaaa agaagttgta
     5461 gaagaacttt taaaagaaaa atttgattat attttcttta ctggaagtgc tactgtagga
     5521 aaaatagtaa tgaaggcagc ttctcaatat ttaactccta taactttaga acttggagga
     5581 aaaagtcctt gtattattga taaggattgt aagctagaat tagcagcaag aagaatagtt
     5641 tggggaaaat tattgaattc aggccaaacc tgtgttgcac cagattattt atatgttcat
     5701 aaagatattg aagaagaatt tataaaaaaa ttagaggaag aaataaaaaa tcaatttgga
     5761 aataatcctt tagaatctga agattattct aaaatggtaa atgaaagaga atttaataga
     5821 gtactttcat atatagataa agaaaaatta gtttttggag ggaattataa tagaaaaact
     5881 tttcaaatag aaccaaccat attaaaaaat gtgacttgga atgatcctgt tatggaaaga
     5941 gaaatttttg gacctatatt cccaatttta tcttttgaaa atttagatga agtaataaga
     6001 ttagtaaata gcaaggataa gcctttagca ttatattatt tttcagagga taaaaataaa
     6061 attgaaaaag ttattaatag tacatcatca ggaggagtaa caataaatga tacattagtt
     6121 catgtatcat catcatatct accttttggt ggagttggaa atagtggtat gggagaatat
     6181 catggtaaat atagttttga tttattttct aataaaaagg gagttatgaa tagaaaaaca
     6241 tttttagatt taaaaattag atatgctcct tttttaaata agttaacaat tgttaaaaaa
     6301 ataatgaaat aaataaaaaa tgagtgaata tggttggatt tgtatggaaa tgatttaaaa
     6361 atttatgtat aatatactta aagattgtat atgttttcaa tgagcggatt aaaagaattt
     6421 aaaataaggt gttcaattta tactaaaaac atataactaa tttaggaata caatctttga
     6481 aagttgcgtc tatgtactaa tgcctgttat ttatagatga acttctgaca aaccaacaat
     6541 attattataa tcatatttaa gattaagttt ttaagcttta aggtttgggg gaactgtata
     6601 gtataattac tactgaaaat atgaataaat tttattttaa acttaagagt aaaaatttgt
     6661 ttttacatga attttataag cttccatgag gaagaagcta gaatatcttt cgacttatga
     6721 atagatatta gggtatttta ttgagaaatt atttttatta ttgaattttg agagaattta
     6781 ttaaatatgg tcatttaaag gggacgttat gagtttaaat taaaatttat aaagttatat
     6841 ttataatatt ggtttgcgaa aaaaggtcta agttatactt agatcttttt ttcttgaaat
     6901 taaccacctt ttttggaatt aagccacata agaaccctag aaaaaagttt ttttaaaata
     6961 tacaattaag tagagaaaaa gtttacaaag gtgtaggggg ttttcacatg aaatatactt
     7021 tcccagaaaa cttttggtgg ggagctgcaa cttcaggacc tcagtcagaa ggtaggttta
     7081 ataaaaaaca tgacaatgta tttgatcact ggttcgatat aaatccagag ttatttcaca
     7141 atggtatagg accaaatata gcatcaaatt tttataatag ttacaaagaa gatttagcaa
     7201 tgctaaaaga aattggatta aattcattta gaacttcaat tcaatggaca agagtaatta
     7261 aggattttga aactggtgat atagatgaag atggagtaag attttataat ggtgtaatag
     7321 atgaatgttt agcaaatgaa atagatataa taatgaattt acatcacttt gatcttcctg
     7381 ttgaattata tgataaatat ggtgggtggg aatcaaagca tgttgtagaa ttatttgcga
     7441 aatttgctaa gactgccttt agtttatttg gtgatagggt taagaagtgg gctacgttta
     7501 atgaacctat agttattatt gaaggacagt ttttatataa atggcattat ccttgtatag
     7561 ttgatggaaa aagagggctt caagctgcct ataatatagc attagcttct gctagagcca
     7621 tagaagagta tagaaaatta ggacaagatg gagaaatagg aataatagtt aacttaacgc
     7681 cagcatatcc aagaagtgag tcaaaagaag atttaagagc tgctgaaatt gccaatgctt
     7741 tcttcaatga gttattctta gatccagcaa ctaagggaga atttcctaag aacttagttg
     7801 aggttttaga aaaagatgga gtaatgtgga attctactaa ggaagaatta caggttataa
     7861 aaaataatac tgtggatttc ttaggggtaa actactatca accaagaagg gttaaagcta
     7921 gagaagagga atatggtgga gaaacatggg ctccagaaaa atattttgat aattatgata
     7981 tgccaggaaa gagaatgaat cctcatagag gatgggaaat atatcctaag gcaatttatg
     8041 atatagctaa aaatgttcaa gagaactatg gcaacataaa atggttcatt tcagaaaatg
     8101 gaatgggtgt tgaaggcgaa gaaaagttca aaaatgctga aggtataatt gaagatgatt
     8161 atagaattga attcataata gagcatttag aatggcttca taaggctatt gaagagggtt
     8221 caaattgtgt gggatatcac ttatggactc caatagattg ttggtcatgg ttaaattcat
     8281 ataaaaatag atatggattt atatcattag atttagaaac tcaaaagaaa actattaaaa
     8341 aatcaggaag atggataaaa gaagtttcta agaataatgg tttttaaaac tttgaattta
     8401 cttagcttag atcagttcta aaataaattt tctaagttaa tatgactacg taaaaagttt
     8461 tttattataa aattataaat aaaataaatt aatatgaata aatgaggaaa ggctttagtt
     8521 atagtgtttt tactaaacta aagccttaaa tttttttaat actcacataa tggaatattt
     8581 tcacataaaa tacactataa caattaacgg agttgaaggc aacatgcttt actcattaat
     8641 tttagcagga ggaaagggta ctagacttta tccactttca agaagttcaa accctaaaca
     8701 atttcttagc atacacaata atgggaagag ttttttaagt aatactgtgg atagaattag
     8761 acctataatc cataaagata atatttatgt agtgacaaac caggagtatg aagacaaaat
     8821 aagagatgaa cttaaagaca taagagagga aaatatcttt actgaacctg ctaataaaga
     8881 aactgcaacc tgcataggtc tttcagcagc taagctttta aagaaagata aggatgcagt
     8941 tatgattgta ttgccttcag accattatat agaaggagat aaaagttatc ttcaaactct
     9001 ggaacaagcc gtagagattg cggataagaa gagagctatt gttaccatag gaatagagcc
     9061 aactagacca gagacaggtt atggctatat agaaatgggc gatagagtca atggacacat
     9121 tcctaccttc aagatagcaa ggtttacaga gaaacctaat ttagaggtag caaaggactt
     9181 cttattaaag gggacttatt tatggaatag tggaatgttc atatttagag ctgatgttat
     9241 attgagagaa attgaaaaat atattcctaa gatgtataaa tgtttaatgg agatatatca
     9301 acacattgga gaagatgatg aggaagaaat aataaaagag cagtattctt taattgatgg
     9361 aatatctata gattttggag taatgcaaaa aacaagaaga tcctttgtta taaaatgtga
     9421 tttttcttgg gatgatatag gtactttctc agctctaagt agatttttag gacctaatgg
     9481 aaataataga acttcagata atgttttttt agaggaatgt gagaattgtt ccgtttttgg
     9541 aaaggaaaaa ttaataatag gatttggaat aaaagatcta gtattagttg atgcaggaga
     9601 tgttattttg gtcatggaca aaggtaggga ccaagagatt aagcatctat taaattctat
     9661 tagtggtaag aaggaattta aagaatatct ataagtttat aaacttatgt aaaaaagtcc
     9721 tatatcatat actttaggtg tatgatatag gactttttag ttgcttttaa tatctacaat
     9781 ttagttaaga aaacataaag ttatttaaaa tttaaattta attaaaatat aactaatttt
     9841 aatccttaaa atatgttata aagatatttt tatgttatta tagtaataat ataagtttga
     9901 aatttatgga gggtatcgtt ttatgagtga tagaattgta gattttaatg agttaaaaaa
     9961 taaggcaaga gataaagata tagataaatt tgagtcatat atttatgatt tatattattc
    10021 agtagctcaa ggaaaaatga gtatgatgga gctttctaga gagataacta aatatatgaa
    10081 tgaaaataat atttctcaag aaaagttttt aaatattcaa acagaaatgc ttaagagata
    10141 tggctttgat atgggtgata ttgaagggca aatgaaaaat ttaggtatag atatgagcaa
    10201 cttaggaatt caaggtggag attatgaaaa tttaagaaaa actttgagtt ttcaagaaaa
    10261 gtataaggga aaaataaaaa catctactgt tactacatat tttattaaaa acgaaaaaaa
    10321 tgatattgaa ttgattttag aaaataataa agttcttata actagtccta aaaatataga
    10381 tcttcaggat agtgaattaa atgagttctt atgttcatat aaaaaaactc tttcagatga
    10441 tcttttagat ataagaattt gtgaaaatat aggacaatat aattattaat ttgtatttta
    10501 taagctagga attgaat
//
LOCUS       DAEVGI010000085        10327 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEVGI010000085 DAEVGI010000000
VERSION     DAEVGI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10327
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.085"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(270..659)
                     /locus_tag="K8M77_001701"
     CDS             complement(270..659)
                     /locus_tag="K8M77_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086195.1"
                     /translation="MNNNKKFKFIVYLDNKELVKQFFYTNIAPFTYYTLPVCGIPAGK
                     VIFEIENRKIYLKAKINDFEKGEHIETLSEIGFEDLDDFSINKTKNKDVYHITMAKKK
                     HKVGLNCSNGLNDDLAKEIVQYVRESA"
     gene            complement(691..1569)
                     /locus_tag="K8M77_001702"
                     /pseudo
     CDS             complement(691..1569)
                     /locus_tag="K8M77_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461986.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1651..1875
                     /locus_tag="K8M77_001703"
                     /pseudo
     CDS             1651..1875
                     /locus_tag="K8M77_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
     gene            complement(2036..2980)
                     /locus_tag="K8M77_001704"
     CDS             complement(2036..2980)
                     /locus_tag="K8M77_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBI7086196.1"
                     /translation="MKKVLCPGEALIDFVSNEHGKALKDTSGFIRKAGGAPANVAAAI
                     SKLGADAYFCGTVGDDFFGEFLEETFKKNNINTEMMIKLKDKSTTLAFVSLKEDGDRD
                     FKFMRNADSDLTFDEIKNNLDQFDLFHFGSATAFLEGELKNTYYKLKEYALKNNKIIT
                     FDANYRDALFGEDKDTFVSCCKDFIKDSKIVKLSDEEACLISGMENIEEAAKYIIDLG
                     CENLIITLGKKGALLTTKEKQILIPTNEMKMVDATGAGDAFIGAVIAQVLDIPNKSIE
                     DIIKTANLVGGITTTRIGALESIPTWDEVKQYSKLDQI"
     gene            complement(3056..4411)
                     /locus_tag="K8M77_001705"
     CDS             complement(3056..4411)
                     /locus_tag="K8M77_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBI7086197.1"
                     /translation="MRRPKLHFTSPKNWINDPNGFIYYKGEYHLYYQYFPYAAEWGTT
                     HWGHATSKDLVNFEHHGIALFPSKKFDSNGCFSGTALIEDDKLQFYYTGIKYLRTEDE
                     NIHKPYDNESFEACQVKIESKDGYTFDNFNDKKVVIPPITDRKLGNKTHTRDPKVWKY
                     KDGYSMIVGSKFEKEGVEGYIGQALFYTSKDGESWEYKNRCYDESIGDMWECPDLVNV
                     DGKYILIISPEHITNDGINYTNNSIYSIVDFDEETCEMKITNGYSYLDEGLDVYAPQT
                     TLDKDGNRILIGWVRMPKKFEGEEWIGMMTLPRVINVIDNKVHFAVTENIQNLFKKEI
                     NRSDFDINNLCRIKVKLNKESHINIGGYKICVEEDSIAVDRSSVFVETDFKAVKFKSS
                     KLDGIYDLDIFVDNGIIEIFINGGKYVITNVVYNMQSYIKYDNINELEIFEINNPDII
                     L"
     gene            complement(4468..5595)
                     /gene="ugpC"
                     /locus_tag="K8M77_001706"
     CDS             complement(4468..5595)
                     /gene="ugpC"
                     /locus_tag="K8M77_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBI7086198.1"
                     /translation="MGFIEFKNVEKQYKNATKKSVTDFNLSIDEKEFIVFVGPSGCGK
                     STTLRMLAGFEEITGGTISIDGNIVNNTPPRERGISMVFQNYALYPHMTVEDNIAFGL
                     KNIKTPKDEIKKKVNWAIEILGLEEYRKRKPKNLSGGQRQRVALGRAIVRNQKVFLMD
                     EPLSNLDAKLRVSMRNEISKLHRELGSTTIYVTHDQVEAMTMADRIVVMKDGIIQQIG
                     TPMDLYDNPRNKFVGSFIGSPQMNFLNVEVKGNKAILENGSKITLPEGILKRMNNRQG
                     KLCMGFRAEDIKLDNLNIGLFEDSIITSAIENTEIMGNENNLYFKIGNTTAVARVGKE
                     DVKEIGEQFKFVINVNKVHFFDLDTEENILNLGNTLTLDYN"
     gene            complement(5631..6482)
                     /locus_tag="K8M77_001707"
     CDS             complement(5631..6482)
                     /locus_tag="K8M77_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBI7086199.1"
                     /translation="MLSNKGKIFNRILIVFMLVLSLLFLFPIIWMIVNSFKSDAMITQ
                     DMNSIAAFLPPLSFDNFFENYITIITNSSLMRYMTNTLVYAAILIVLSIIVNGLAGYA
                     LAKINFPFKEVWVFIILLLMIVPMETISIIHFLMIAKAGLLNTVVGYILPMIVSPFNI
                     FLFRQVFMKLPDDLYEAAQLDHCGPLKYFFRVVIPMSKSIVATVGVFTFLGIWNDFLW
                     PSLVFTSSNLLTVQIGLNSITANDNVTTGQVLATITLVTIPILIVYSLFSKQLVEGAM
                     SSGSKEG"
     gene            complement(6484..7353)
                     /locus_tag="K8M77_001708"
     CDS             complement(6484..7353)
                     /locus_tag="K8M77_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI7086200.1"
                     /translation="MNKIREKIMGMAFFGPALVLLTLFLFIPMILTLIFSFTDFFTLN
                     PSATKFVGLRNYTRLFNDELFGKAFFNTIKFALVVVPLQMGGALLLALGINKVTHCKK
                     YFKIAFFIPVVMSLAVVSTLWMQIYSPEGILNNMLNAIGISSQPFIFSKDQALNSISA
                     LSIWQGMGYQMIIFLGGLQAISPSLYEAAEIDNASGWGKFWNVTVPELKPISIYILLT
                     ITIGAFKLLVQPMVMTGGGPAFSTYSLVYYIYDTGTVNWDMGYSSAMAIVFAIIVIAL
                     AGIQYKLTNKKED"
     gene            complement(7375..8694)
                     /locus_tag="K8M77_001709"
     CDS             complement(7375..8694)
                     /locus_tag="K8M77_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBI7086201.1"
                     /translation="MKKFYGFVLLIFCFNLFGCSFRNTEVSNKNIQGYDKGEELITMW
                     VHVIEETSEGQAYKNSVERFNKEYNGKYCLSVEFVPRNESGGGYTDKINSSVISGGLP
                     DIITVDGPNVSAYVANNIIQPLVGITDDEKAKYLPSVIEQGTINNKLYALGLMESSTL
                     FYYNKDILNEVGIQVPSFDNPWTWDELNKVCEKVKNYLDKKNGYPIDMSFPAGETTIY
                     FYAPFIWSNGGDFVSSDGLKVNGVFNSEKNVETISYFKEITDKGYIPKYTISDLFEKG
                     RAAFKFDGAWAITNIRNNYPAFNLGIAPYPVGNDWNGEKYTPTGGWAFATTTTCKNPE
                     AAKEAIKFLTNAESGIDMYNLTGNLPSTFEAYENIDAFKTDELFKTAYYQLVNYGHPR
                     PKSPAYPQISTSYQQAIEGVLLNDETPEESLYKTMRRIEDKLIRYQD"
     gene            8927..9946
                     /locus_tag="K8M77_001710"
     CDS             8927..9946
                     /locus_tag="K8M77_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7086202.1"
                     /translation="MKKNITFSDIAKYTNFSKTTISRYFNSPETLTQASIQKIEKALS
                     ELNYTENKVARSLANGKTEIIGIIIPNLFLHYYSQILNNIIDTYSKYNYKFLVFLGNS
                     DPNEEIKYIKELMAYKIEALIILSHSISSHQLKEFNIPIVAIEREDNYISSVNSDNYS
                     GAEKATKLLIENNCDILIHINGKINCNTPAYKRIEAFKETCQRNNQDFKIYMSDIKSS
                     FEESYNSLYQIYLDIEKNYPYVKKGVFLSNDTHANIFLNILIRNKKSIPDEYELIGFD
                     NSPISTEAIIPITTIGQNIDKISDYAIKSLLRQINLKKNNKIAPIEHIIVPTELIVRD
                     TTSKY"
     gene            9995..10234
                     /locus_tag="K8M77_001711"
     CDS             9995..10234
                     /locus_tag="K8M77_001711"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7086203.1"
                     /translation="MGRGKKYTEDYKEIISDLYKSDMTISEISSEYGIAKSTINGWVK
                     SNKEIKISEDEVITLKEVAKLKKEMARIKEENEIL"
BASE COUNT         3438 a         1719 c         1171 g         3999 t
ORIGIN      
        1 tataagtgta tccttatatt atttacctta atccacatct aaaattttgg agaaatactt
       61 cttgcttctc tttgctgcaa tggttatttt gaagggactt ttgtcgtagc cttatacaat
      121 gatgtttcct gatttatctc tgtaaactac ttgtatattt ttaaagtaga aaatgtagta
      181 gccaagtata attccatctt taaaggagcc tgttgctcta tttagtgctt catttccaaa
      241 tatgaattta acttctttga actatgtatt taagcactct ccctcacata ctgaactatt
      301 tccttagcca gatcatcatt taaaccattg ctacagttta gtcctacttt gtgttttttc
      361 ttggccattg taatgtggta aacatcctta ttttttgttt tattaatgct aaaatcatct
      421 aaatcctcaa agcctatttc acttaaggtt tcaatgtgtt ctcccttctc aaaatcattt
      481 atctttgctt ttaagtaaat ctttctattt tctatttcaa aaataacctt gccagcaggt
      541 atcccacata caggtaaagt gtaatatgta aagggggcaa tgtttgtata aaaaaattgc
      601 tttactaact ccttgttgtc taaatatact ataaatttaa attttttatt gttattcata
      661 ctatttacct ctttaatata attaatttta ttatttatta gtattattgc taagctttgg
      721 gaactgttta gttggtacaa aactcttctt tgactcttca attatcttat taaacacatc
      781 tttatattta actggatatt tataaataaa tgtatttaca tcatccccct taatgctaat
      841 atacccataa tgatattcca taaaaccatc tttctctaca ataaatgaaa atgtatttcc
      901 ctcttgattc ttatttataa ctttaccttc ttttttaaaa gtctcaatta agttttcaaa
      961 ttattcttta gcatcattcc ccatattatt catatatgaa cctactgaaa actccatctt
     1021 tttatcttta gatctaagtg ttattccaaa ttctaaattg gggacttcat ctttttgtat
     1081 ttcaaaaaag ttaggataat ttattgaaaa tcccttcata ataaatgtat tgtaactcca
     1141 atctttgtca atatttccaa cctccaaagg atttttacct attatagctc cataaatatt
     1201 ttctttgtct aaatcagaat aattttcaat ttcattaagt tctactatat tctttctatt
     1261 gtaatcaaat gttatttcag caatcttaaa cccctttggt atagttataa ctccatcctt
     1321 tacagtaaca acttctttat tttctgaaga tttttcttct ttttttgctt tagtatcatt
     1381 ttctgaatcc tttgattcct ttttatcctt attatcattt ttaacttctt ctttattatt
     1441 cttttcctta ctagaaatat tagagctgtt ttcattatca cttacttctt tttgattgtt
     1501 tgtgctacaa ccaatgaatg caccgcttaa tattcctatt gaaagtaata gacttactaa
     1561 tcttctcata atattatcct atatttaaca atttattaac attatataac atttttgtta
     1621 ataagttaag tttattactc tttgcaaata aaaaaatata tagaaagcta tgaacttgaa
     1681 aatgctcttg tagaaataga tattaacgat aaaagctttt gccctttccc ttatattgaa
     1741 atagaaacag attccattga aaatttagaa aaggttgttg cttatcttgg atatacttta
     1801 gaaaatacta cttctcaaac tatttatgat attttagctg aaagaggaat cactggaagt
     1861 actcttggaa aataataagt ttaaaatgta tactattata taaaaatagc tagatattgg
     1921 ataattccag gtaatctagc tatttttatt acttatacat gacacaatta tctaagcata
     1981 ttctaatgtt aaaattgcaa acttttattt tgtatatcta cacttttgaa atatactata
     2041 tttgatctaa tttactatat tgttttactt catcccatgt tggaattgat tctaatgcac
     2101 ctattcttgt agttgtaatt cctccaacta aattagctgt ttttattata tcttctatgc
     2161 ttttattagg aatatcaagc acttgtgcaa taacagcacc tataaaagca tcaccagcac
     2221 ctgttgcatc taccattttc atttcgtttg ttggtattaa tatttgtttt tctttagttg
     2281 taagaagtgc acctttttta cctaaagtta ttattaagtt ttcacaacct aaatctatta
     2341 tatatttagc tgcttcttct atgttttcca tgccacttat taaacatgct tcttcatcac
     2401 taagtttaac tattttactg tcttttataa agtctttaca gcaacttaca aaagtatctt
     2461 tatcttcacc aaataaagcg tctctgtagt ttgcatcaaa agttattatc ttattatttt
     2521 ttaatgcata ttcttttagc ttgtaataag tattctttaa ttctccttca agaaatgctg
     2581 ttgcacttcc aaaatgaaat aaatcgaatt gatcaagatt attctttatt tcatcaaaag
     2641 ttaaatctga atctgcattt ctcataaact taaaatctct atccccatct tctttaagtg
     2701 aaacaaaggc taatgtagtg cttttatctt ttaatttaat catcatttca gtattaatat
     2761 tatttttctt aaaggtttcc tctaaaaact ccccaaaaaa atcatctcca actgttccac
     2821 aaaaatatgc atctgctcca agcttagata ttgcagcagc aacattagct ggtgcacctc
     2881 cagcttttct aataaatcca cttgtatctt ttaaagcttt tccatgctca ttagatacaa
     2941 aatcaattaa tgcttctcca gggcaaagta cttttttcat cttccattcc tccaataata
     3001 tgctagataa gagtactact attttaaaaa tgcggtactc ttaaactttt tcaatttata
     3061 aaataatatc aggattattt atctcaaata tttccaattc attaatatta tcatatttaa
     3121 tgtatgattg catattgtat actacatttg taattacata ctttccacca tttataaaga
     3181 tttcaattat tccgttatct acaaatatgt ctaaatcata tattccatct aatttagatg
     3241 atttaaattt tactgcttta aaatcagttt ctacaaacac actgcttcta tcaactgcta
     3301 tgctatcctc ttctacacat attttgtatc ctccaatatt tatatgactt tctttgttta
     3361 gcttcacttt tattctgcat agattattaa tatcaaaatc tgatctattt atttcttttt
     3421 taaataagtt ttgtatattt tctgtaactg caaaatgtac tttattatct attacattta
     3481 ttactcttgg aagagtcatc atacctatcc attcttcacc ttcaaacttc ttaggcatac
     3541 gaacccagcc aattaaaatt ctgtttccat ctttgtctaa agttgtttga ggtgcataaa
     3601 catcaagccc ttcatctaaa taactatatc catttgttat tttcatttca caagtttctt
     3661 catcaaaatc tacaattgag taaatagaat tatttgtata gtttatccca tcatttgtta
     3721 tatgttcagg agatataatt aaaatatatt ttccatctac attaactaaa tctggacatt
     3781 cccacatatc acctatactt tcatcgtagc aacggttttt gtattcccaa gattctccgt
     3841 ccttacttgt gtagaaaaga gcctgtccta tatatccttc tacaccttct ttttcaaact
     3901 tacttccaac tatcattgaa tatccatctt tatatttcca aacctttgga tctcttgtat
     3961 gagttttatt acctaatttt ctatcagtta taggtggaat aacaactttt ttatcattaa
     4021 agttatcaaa tgtatatcca tccttacttt ctattttcac ttggcacgct tcaaatgatt
     4081 cattatcata tggtttatga atattttcat cttcagtcct taaatattta attccagtat
     4141 aataaaattg tagtttatca tcttctatta atgctgttcc tgagaaacat ccgttactgt
     4201 caaatttctt acttgggaat aatgcaatac cgtgatgttc aaaatttact aaatccttac
     4261 tagttgcgtg tccccaatgt gtagttcccc actctgcagc atatgggaag tattgatagt
     4321 ataaatgata ttctccttta taatatataa atccgttagg atcatttatc caatttttag
     4381 gtgatgtgaa atgtaattta ggtcttctca ttaatttccc tcctagttct ctaaagttag
     4441 agtatttctt aattttaata tattttttta attataatct aaagttaggg tatttcctaa
     4501 gtttagtata ttttcttcag tatccaagtc aaagaaatga actttattta cattgattac
     4561 aaatttgaat tgctctccaa tttcctttac gtcttctttt cctactcttg ctactgctgt
     4621 agtgtttcct attttaaaat acaagttatt ttcatttccc atgatttctg tattttctat
     4681 agctgaagta ataatactgt cttcaaataa tccaatattt agattatcaa gctttatatc
     4741 ttcagctcta aatcccatac ataatttgcc ttgtctgttg ttcattcttt ttaatattcc
     4801 ttctggaagc gttattttgc ttccattttc taatatagct ttatttcctt taacttcaac
     4861 attaagaaag ttcatttgtg gtgagcctat gaagcttcca acaaatttgt ttctaggatt
     4921 gtcatataag tccataggtg ttcctatttg ttgtattatt ccatctttca taacaacaat
     4981 tctatctgcc atagtcatag cttcaacctg atcatgggta acataaattg tagtacttcc
     5041 aagttctcta tgcaatttac ttatctcatt acgcatactg acacgtaatt tagcatctaa
     5101 attacttaaa ggctcgtcca ttaagaatac tttttgatta cgtactattg ctcttccaag
     5161 tgcaaccctt tgacgttgtc ctccagataa attcttaggc ttacgctttc tgtattcttc
     5221 taaacccaaa atctcaattg cccagtttac ttttttcttt atttcatctt ttggagtttt
     5281 aatattcttt aatccaaaag ctatattatc ttctactgtc atatgaggat ataatgcata
     5341 gttttggaac accatagata ttccacgttc tcttggcggc gtattattga ctatatttcc
     5401 atctattgaa atagttcctc cagttatttc ttcaaaacct gcaagcattc ttaaagttgt
     5461 tgatttacca catcctgatg gtcctacaaa tactataaat tctttttcat ctatggataa
     5521 attaaaatca gtaactgact tctttgttgc attcttatat tgtttttcta cattcttaaa
     5581 ttctataaaa cccattttat cgcctaacct ttcttttatt tatataaatt ttagccctct
     5641 tttgatcctg atgacattgc tccttctact aattgcttag agaaaagaga atatactatt
     5701 aatattggta ttgtaaccag tgttatagtt gcaagtacct gacctgttgt aacattatca
     5761 tttgcagtaa ttgaatttaa tcctatttga actgttaata aattgcttga tgtaaacacc
     5821 aatgatggcc ataaaaagtc attccatatt cctaagaatg tgaatacccc tactgttgct
     5881 actatagact ttgacatagg tataacaact ctgaaaaaat attttaatgg accacaatga
     5941 tctagctgcg ctgcttcata taaatcatct ggaagcttca taaatacttg tctaaataag
     6001 aatatgttaa acggacttac aatcatagga agaatatatc caactacagt atttaatagt
     6061 cctgcttttg caatcattaa aaagtgaata atacttatag tttccatggg tacaatcatt
     6121 aataatagta ttataaatac ccatacttct ttaaatggaa aatttatttt tgctaaagca
     6181 tatcctgcta gaccattaac tataatactt aaaactataa gtattgctgc ataaactaac
     6241 gtattagtca tatatcgcat taaactgctg tttgtaataa tagttatata attttcaaaa
     6301 aagttatcaa agctaagtgg tggaagaaat gctgctatac tgttcatatc ttgtgttatc
     6361 atagcatctg atttaaaaga atttacaatc atccatataa taggaaataa aaacaatagt
     6421 gataatacaa gcataaaaac tataagaatt ctattaaaaa tttttccttt atttgaaagc
     6481 atattaatcc tcctttttgt ttgtaagttt atattgaata cctgcaagtg caattactat
     6541 aattgcaaat acaattgcca ttgctgaaga atatcccata tcccaattaa ctgtaccagt
     6601 atcatagata tagtacacaa gtgaatatgt agaaaaggct ggtcctcctc ctgtcatcac
     6661 cataggctga actaataatt taaatgctcc tattgtaatt gttaataata tatatattga
     6721 aattggttta agctctggaa ctgttacatt ccaaaatttg ccccatccac ttgcattatc
     6781 aatttctgct gcttcatata aactaggaga aatagcctgc aagccaccta gaaatataat
     6841 catctgatat cccattcctt gccatatact taatgctgat atagaattaa gtgcttgatc
     6901 tttactgaat ataaatggct gagatgatat tccaatagca tttaacatat tatttaatat
     6961 accttcaggg ctataaatct gcatccacaa agttgatact actgcaagtg acattactac
     7021 tggaataaaa aatgctattt tgaaatattt tttacaatga gtaactttat ttatgccaag
     7081 tgctaaaagt aatgctcctc ccatctgcaa tggtacaact acaagtgcaa attttattgt
     7141 attaaaaaat gcttttccaa ataactcatc gttaaataat cttgtataat ttctaagtcc
     7201 aacaaactta gttgcgctag gatttaatgt aaaaaagtct gtaaaactaa atatcaaagt
     7261 taaaatcata ggaataaaca aaaataatgt tagtaacact aatgctggac caaaaaaagc
     7321 cattcccatt atcttttctc ttatcttatt cattactttc cacctccaat tatattaatc
     7381 ttgataacgt attaacttat cttctattct tctcattgtt ttatataacg attcttctgg
     7441 tgtttcatca tttaatagta caccttcaat agcctgctga tatgatgtac ttatctgagg
     7501 ataagctggt gattttggtc ttggatgacc atagttaaca agctgataat atgctgtttt
     7561 aaataattca tcagttttaa atgcatcaat attttcataa gcttcaaatg tagatggtaa
     7621 attacctgtt aagttataca tatctatgcc actttctgca ttagttaaaa acttgattgc
     7681 ttcttttgca gcctcagggt ttttacaagt tgtagttgtt gcaaaagccc atcctcctgt
     7741 tggtgtatac ttttctccat tccaatcatt tcccactgga tatggtgcta ttcctaaatt
     7801 aaaagctgga tagttatttc ttatatttgt aatagcccat gctccatcaa atttaaatgc
     7861 agcccttccc ttttcaaata aatcacttat tgtatattta ggtatatatc ctttgtctgt
     7921 aatttcttta aaataactaa tagtttctac attcttttca gaattaaata ctccattaac
     7981 ctttaaacca tcagagctta caaaatctcc accatttgac catataaatg gtgcataaaa
     8041 ataaatagtt gtttcccctg ctgggaatga catatctatt ggatatccat tttttttatc
     8101 taaatagttc ttaacttttt cacagacctt atttaattcg tcccaagtcc atggattatc
     8161 aaatgatggt acttgtattc ctacttcgtt taatatatct ttgttataat aaaataacgt
     8221 actagattcc attagaccta atgcatataa tttattgttt attgttcctt gctctattac
     8281 tgacggtaaa tatttagcct tttcatcatc agttatacct actaaaggct gaattatgtt
     8341 gtttgcaaca taagctgata cattgggccc atcaacagtt attatgtctg gaagtcctcc
     8401 agaaattact gatgaattta ttttatcagt atatcctcct ccactttcat tacgaggtac
     8461 aaattcaaca cttaaacaat acttaccatt gtattcttta ttaaaccttt ctactgaatt
     8521 tttataagct tgtccttctg aagtttcttc tataacatga acccacattg ttataagctc
     8581 ctcgcctttg tcatatcctt gaatattttt atttgatact tctgtatttc taaaagaaca
     8641 tccaaaaaga ttaaaacaaa atattagtaa aacaaaacca taaaatttct tcactattca
     8701 ttcctcctat tttaaatcac cggtaatgta accggttatc ttaattaaag tatatcaaga
     8761 aaacatttac aaatcaatat atttttaata tttttgctga aattaatatt taatgatata
     8821 atttcattaa ttttaatatt cttttacttt acatagtttt aatacatatt ttaaaccttg
     8881 tatttataag cttaatataa ggattattga aataggaggt aattacatga aaaaaaacat
     8941 cacttttagt gatatagcta aatatacaaa tttttctaag acaacaatat caagatattt
     9001 taattctcca gaaactttga ctcaagcttc aatacaaaaa attgaaaaag cattatcaga
     9061 attaaattat actgaaaata aagttgctag aagtttagca aatggtaaaa ctgaaattat
     9121 aggaattatt atccctaact tatttcttca ctattattct caaatattaa ataatataat
     9181 agatacctat agtaaatata attataaatt tttagtattc ttaggaaatt ctgatccaaa
     9241 tgaagaaata aaatatataa aggaactaat ggcttataaa attgaagcgc ttataatatt
     9301 aagtcattct ataagttcac atcaattaaa agaatttaat attccaattg tagccataga
     9361 acgcgaagac aattatatat caagtgtaaa tagtgataat tattctggtg ctgaaaaagc
     9421 aactaaatta ctaattgaga ataactgtga tatattaata catataaatg gaaaaataaa
     9481 ttgcaatact cctgcttaca agcgtattga agcatttaaa gaaacttgcc aaaggaataa
     9541 tcaggatttt aaaatttata tgtcagacat taaaagttct tttgaggaaa gctataattc
     9601 tctttatcaa atttatttag atattgagaa aaattatcct tatgtaaaaa aaggagtatt
     9661 tctaagtaat gatacacatg caaatatctt tctaaatatt cttataagaa ataaaaaaag
     9721 tataccagac gaatacgaac taataggatt tgataattct ccaatttcta ctgaagcaat
     9781 aattcctata acaacaatag gtcaaaatat tgataaaata tctgactatg ctataaagtc
     9841 tctattaaga caaataaacc ttaaaaaaaa taataaaatt gcacctattg agcatattat
     9901 tgtaccaact gaattaatag ttagagatac tacaagtaag tattaaattt tccaatgaat
     9961 attagttttt attatttggc tctgttaaac taccatgggt agaggtaaaa aatatactga
    10021 agattataaa gaaattatat cagatttata taaatctgat atgacaattt ctgaaataag
    10081 tagtgaatat ggcatagcaa aatcaactat aaatggttgg gtaaaatcta acaaagaaat
    10141 aaaaatatct gaagatgaag tcataacatt aaaagaagta gccaaactaa agaaagaaat
    10201 ggctagaatt aaagaggaaa atgaaatatt atagtaagaa aaagccgaaa gaaggtttag
    10261 aaaatctttt gaagcgtgat tttacaacaa caagcataaa tgagaaatgg gtaggagata
    10321 taactta
//
LOCUS       DAEVGI010000086        10267 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEVGI010000086 DAEVGI010000000
VERSION     DAEVGI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.086"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            120..827
                     /locus_tag="K8M77_001712"
     CDS             120..827
                     /locus_tag="K8M77_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086204.1"
                     /translation="MVILDSKVGHIKSRISKDRVVLKTMYPFKKGELADEVEINLYLE
                     GSNRVIKKQLPYGGYNMHLFLGDFLGSGRDCILVKGGFQGSGGIAILLLYEYDNGEIR
                     EITNQWEISARNKAIIRYLPNYKVEVSYGAKEEYIVDLSSKDKSYLNLIYDEKGNVKL
                     KINPGISDINTYYEIKELGSNKYDFLIRQNIIGIVLVDVIGIIQSRVIFNINGNFVIK
                     DREFVISKDRNLNNTHN"
     gene            921..2900
                     /gene="glgB"
                     /locus_tag="K8M77_001713"
     CDS             921..2900
                     /gene="glgB"
                     /locus_tag="K8M77_001713"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HBI7086205.1"
                     /translation="MRNYKELKHEKNGNVTEKIGENKGKSKKMSKDESLLSFDLFLEG
                     KEHSAYKFMGAHFITENRKRGVRFTTWSPRASKIYIIGDFNNWELKEEYSMKKINERG
                     IWSLFIPKLEEGIKYKFAVENECGNNTVYKSDPYAFKSELRPNTASVLTKIKSFRWGD
                     KRWLNKREKEGLDNKPMNIYELHLGSWKRKDGEFMTYEEISEVLVEYIKEMGYTHVEF
                     MPVNEHPLDASWGYQGVGYYSVTSRYGDLNGLKTLINKLHKNNIGVLLDWVPSHFCKD
                     EHGLFMFDGSPTYEYEVWWKANNEGWGTCNFDLGRPEVKSFLFSNAMYWINEFHVDGL
                     RVDAVSNMLYLDYGREYGEWEPNIYGENGNLEAIAFLKELNTIIKKEGKGAITVAEES
                     TSWEGITKSVEEGGLGFDYKWNMGWMNDTLSYIELDPIYRKYHHNKMNFSMMYNYSEK
                     FILPISHDEVVHGKKSLINKMWGDDWKKYAGLRLYASFMMGHPGKKLMFMGCEFGQFV
                     EWREWEELQWSVIEEFDIHRKTKEYFKALNKFYLENSSLWSLDYEEEGFKWMDADNSE
                     ESVLSFIRIGKNKKEKLIFICNFTPEVYYDFKVGVPELGEYVEVFNSDSLEFGGVGNI
                     MGDSILKATEESFKDFDYSISVKVPPLGTLVLKVK"
     gene            3000..6194
                     /gene="pulA"
                     /locus_tag="K8M77_001714"
     CDS             3000..6194
                     /gene="pulA"
                     /locus_tag="K8M77_001714"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HBI7086206.1"
                     /translation="MFDSVKRKLVCYDDNEYYIEISPLTNTIRATLCCKGAIEAYLVG
                     DFNNWEKSENFKLNWGLDTNDGRVKMIKDITFPCGLKKGEYKYGYIIITLDGKEIYVD
                     NLNEEKNDFYFLWEPFEESLEIKSSRDFISTRYPVELIAVKNSFYGNITIEEEVLSIE
                     NSLKGVTLENGFLKINEEVASGTKIIVKAYDSLNDMTAYKEIEVREDGEKGTFVQFLK
                     NDGLYYGENFSWNIWGFGENSSGKEFNLNIKTDLGVGAFVDEEKFIVRRRTWGYDWVN
                     DWDEQTYTFYLGKEDRNLFCIYENNKILTSLKTAIEETTPKIQVALMDHKNTIKAYLS
                     HKPLIGVKYGLYINGIKAKGVSTLVRENEKEVLITNLPSDIDPSDLLEVRASSMFSSC
                     KVIVRDYLNDYYYGGNDLGVRFSEENISLRLWAPTAKKVELLIYEDYKSLRENPLRKY
                     DMNREKENGTHLIKVPRKENEGKYYLYRLYFNGLSKKGKHVNKITYAVDPYAVSVGVN
                     GEKGALVDLFNKECVPEGWNKYNKPKLINKEDSIIYEMHIRDFTINENSGVSESLRGK
                     FLGTVEEGTFYIDKESGNKVKTGLDHLKELGITHVHLLPVFDFASVNEEITKDENNRN
                     WGYDPKNFNAIEGSYSTDPYTPSRRIIEFREMIKKFHDNGIRVILDMVYNHMYETSNM
                     DNIVPLYYFRSDKLGKYTNGSGCGNEMASEKPMVRKFILDSILHWIKNYHIDGLRFDL
                     MELIDLDTMKEIVKRSEEIDEKILIYGEPWKGGDSPLSNGTYKGSQKGLGFSIFNDDF
                     RNALRGNNDPSNGFINGEQHNKNKVWQVIEGIKGSINSITYKPMESINYLEAHDNYTL
                     WDQIVKSQNHSVEKGHYRDFNEENILDNFYVKEDLLGASILFTSQGIPFIQSGAEFLR
                     SKDGDHNSYKSPDSINALNWAEKEKYIDVFNYYKDLISLRKNHSAFRMKNPEDIIGNL
                     EVYFYDNNDTSGVIIAHYKNNANEDLWKDIVVIYNGTTIDDYNVISSMPKSSNGFWNI
                     AVKSWALNQFGIERVSEDEIPKIKSHSMMILYDE"
     gene            complement(6352..6804)
                     /locus_tag="K8M77_001715"
     CDS             complement(6352..6804)
                     /locus_tag="K8M77_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBI7086207.1"
                     /translation="MKKISLLLLSLTLTASLFIGCGSNNTESKDANTQTSSESSSKIQ
                     SEEVSRDISIDESKKLINQGEVTLILDVRDADEFAKGHLKNAIQIPVKELKENLNDIE
                     KFKDELVLVYCRSGKKSAEAINILKENGFKNLVHMKDGISKWDGEVEK"
     gene            7006..7563
                     /locus_tag="K8M77_001716"
     CDS             7006..7563
                     /locus_tag="K8M77_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HBI7086208.1"
                     /translation="MKKNKLSIKTIVAIGIGSAVFMILGRFGSLPTGIPNTNIETAYA
                     FLSLMALLYGPLAGFLIGFIGHALKDIVFFGSPWISWVFASGIVGLIIGFGARFIKIN
                     QGVFKLKQIFMFNLIQIIANGVAWFLVAPTLDILIYSEPLNKVYLQGVIGGISNMITV
                     GVLGTVLISNYSKTRIKKGSLRKEY"
     gene            7708..9414
                     /locus_tag="K8M77_001717"
     CDS             7708..9414
                     /locus_tag="K8M77_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086209.1"
                     /translation="MERKAIIEFKDFTAHYTVQSKPTLNNINLTIYEGEKVLIAGPSG
                     SGKSTLANCINGLIPFSTDIEISGSLKIKGKETKDLSVFEISKMVGTVLQDPDSQFIG
                     LTVAEDIAFKLENNCVSQEEMKKKVNYVSKIVDIENRLDLAPYSLSGGQKQRVTLAGT
                     IIDDVDILLFDEPLASLDPAAGKASIELIDKIQKEEKKTVIIIEHRLEEVLNCDVDRI
                     ILMNEGEIIADTTPNEILASNKLKECGIREPLYITALKYSGYELNKEMNLENIDRLKL
                     SDDGEKLRTWYKSLNFNKKEREEDTLLEFKNVSFSYDKKKDILNDINFKINRGDMVSV
                     VGKNGAGKSTISKLICGFFKESKGDIIFEGKSLDGKTIKERGEEIGVVLQNPNQMISK
                     TMIFDEVALGLRVRGIDEKEVKERVIETLKTCGLYPYRNWPVSALSFGQKKRVTIASI
                     LVLNPKVIILDEPTAGQDYKHYNEIMEFLLKLNKKGVTIILITHDMHLMLEYTDKAIV
                     ISNGRKIADKTSAEILTDKEVIEKANLKETSLYDLALKFKVENPKDFVCKFIDFDRGV
                     RN"
     gene            9414..10244
                     /locus_tag="K8M77_001718"
     CDS             9414..10244
                     /locus_tag="K8M77_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBI7086210.1"
                     /translation="MDAMLTYIKKDSPIDKLTGATKLICFIFWTLAAMLTYDTRVLIG
                     MLIFSLIIFKVSKIEFKEVSFIVYFILLFLLLNDLAIFIFSPYEGVNIYGTRYDIFHI
                     IGPYTLTWEQLFYEFNVTLKYLSVIPMALLFILATNPSEFAASLNKIGVSYKISYSVS
                     IALRYIPDIQREYHDISFAQQARGIDMSKKEKLGKRIKNMSSILMPLIFSSLDRIENI
                     STAMELRAFGKNKKRTWYNERKFSKGDYLAIIVVILIVALSLYFTFNNGSRFYNPFIK
                     "
BASE COUNT         4045 a          995 c         1880 g         3347 t
ORIGIN      
        1 aaaaatatgt ttttttaatg aaaaatagtt aatagatttg tatttataat ttagttacta
       61 tagttaaaat tttgcataga ttaattatga atggaattta ttagggagga taaactataa
      121 tggttatttt agattctaaa gttggacaca taaaaagtag aatttctaaa gacagggttg
      181 tattaaaaac aatgtatcca tttaaaaaag gagaattagc agatgaagtt gaaataaatt
      241 tatacttaga aggaagtaat agggtaataa aaaaacaatt gccttatggt ggatataata
      301 tgcatttgtt tttaggagat tttttaggaa gtggaagaga ttgtatattg gtgaagggag
      361 gatttcaagg ttctggtgga attgctattt tattattata tgaatatgat aatggagaaa
      421 taagagaaat aactaatcag tgggaaataa gtgctagaaa caaagctatt ataagatatt
      481 taccaaatta taaagtggag gtatcttacg gagcaaaaga agaatatatt gtagatcttt
      541 cttcaaagga taaatcttac ttaaatctta tatatgatga aaagggaaat gtaaaattaa
      601 aaattaatcc tggtatttct gatattaata cctattatga aataaaagaa cttggaagta
      661 ataaatatga ttttttaata cgtcaaaata tcataggaat agtactagta gatgttatag
      721 gtataataca aagtagagtt atatttaata ttaatggaaa ctttgtcata aaagataggg
      781 aatttgtaat aagtaaagat agaaatttga ataacaccca taattaattg taatttaata
      841 cctaaaatag tataattaaa gaaaaatatt gtgtttatga tattttgttt taatgagagg
      901 tttactatgg ggggaaagag atgagaaatt ataaagaact taagcatgag aaaaatggta
      961 acgttaccga aaaaataggg gaaaacaaag gaaaaagtaa aaaaatgtct aaagatgaaa
     1021 gtttattaag ctttgatttg tttttagaag gaaaagaaca ttcagcttat aagtttatgg
     1081 gagctcattt tataactgaa aatagaaaga ggggtgtaag atttactact tggtctccaa
     1141 gggctagcaa aatatatatt ataggtgatt ttaataattg ggaattaaaa gaagaatact
     1201 ccatgaaaaa aattaatgaa agaggaatat ggagtttatt tattcctaaa ttagaagagg
     1261 gtataaagta taaatttgca gtggaaaatg aatgtggaaa taatacagta tataagtctg
     1321 acccatatgc atttaaatca gaattgagac ctaatacagc ctctgtactt actaaaatta
     1381 agagttttag atggggagat aagagatggc ttaataaaag agaaaaagaa ggtttagata
     1441 ataaacctat gaatatatat gaacttcatt taggttcttg gaaaagaaaa gatggagagt
     1501 ttatgaccta tgaagaaata agcgaagtac tagtagaata tataaaagag atgggataca
     1561 cccatgttga gttcatgcct gtaaatgaac atcccttaga tgcttcttgg ggatatcaag
     1621 gagtagggta ttactcagtt acaagtagat atggagattt aaatggatta aaaactctta
     1681 taaataaact tcataaaaat aatattggag tattactaga ttgggtacct agccattttt
     1741 gtaaggatga gcatggatta tttatgtttg atggatcacc aacctatgaa tatgaagttt
     1801 ggtggaaggc taataatgaa ggatggggaa catgtaattt tgatttagga agaccagagg
     1861 ttaaatcctt tttattttct aatgctatgt actggataaa tgagtttcat gtagatggat
     1921 taagagtaga tgcggtttct aatatgcttt atttagatta tggaagagaa tatggagaat
     1981 gggaaccaaa tatctatgga gaaaatggaa accttgaagc tattgccttt ttaaaagaac
     2041 taaatacaat tataaaaaaa gaaggaaaag gggcaattac agtagctgaa gaatcaacct
     2101 catgggaagg aattactaag tctgttgaag aaggtggatt aggatttgat tataaatgga
     2161 acatgggatg gatgaatgat actttaagtt atattgagtt agatccaata tacagaaagt
     2221 atcatcataa taaaatgaac ttttcaatga tgtacaatta ttcagaaaag tttattctac
     2281 caatttcaca tgatgaggtt gttcatggaa agaaatctct tataaataaa atgtggggag
     2341 atgattggaa gaagtatgca ggactaagac tttatgcttc ttttatgatg ggacacccag
     2401 ggaaaaaatt aatgtttatg ggctgtgaat ttggtcagtt cgttgaatgg agagaatggg
     2461 aagaacttca atggagtgtt attgaagaat ttgatataca cagaaaaacc aaggagtatt
     2521 ttaaagcatt aaataaattt tatttagaaa atagttcttt atggtcactt gattatgaag
     2581 aagaaggctt taaatggatg gatgctgata attcagaaga aagtgtactt tcatttataa
     2641 gaattggaaa gaataaaaag gaaaaattaa tatttatatg taattttact ccagaggttt
     2701 attatgattt taaagtaggt gttccagagc ttggagaata tgttgaggtc tttaactcag
     2761 attccttaga atttggagga gtaggaaata ttatgggaga tagtatttta aaagccacag
     2821 aggaaagttt taaagatttt gattattcaa taagtgttaa ggtacctcca ttaggaactt
     2881 tagttttaaa agtaaaataa gaaagaaaag gctactataa atctaagtac ataatattat
     2941 tttacttttg ataaatagta gcttttatta cataaataat taatagagga gcattttata
     3001 tgtttgattc tgtaaaaaga aaacttgttt gttatgatga taatgaatat tacatagaga
     3061 ttagtccttt aactaatacc ataagagcaa ccttatgttg taagggggca attgaggctt
     3121 atttagttgg agattttaat aattgggaaa aaagcgaaaa ttttaagctt aattggggat
     3181 tggatactaa tgatggtaga gtaaaaatga ttaaggatat tacctttcct tgtggactaa
     3241 agaagggaga gtataagtat ggatatataa ttattactct agatggaaaa gaaatatatg
     3301 tagataactt aaatgaagag aaaaatgatt tttatttcct ttgggagccc tttgaagaat
     3361 ccttagaaat aaaaagttca agagatttta tatctactag atacccagtt gaattaatag
     3421 ctgttaaaaa ttcattttat ggaaatataa ccattgaaga ggaggttctt tcaatagaga
     3481 attctttaaa gggagtaact ttagaaaatg gttttttaaa gattaatgag gaagtagcct
     3541 ctggcactaa aattatagta aaggcctatg attctttaaa tgacatgaca gcttataagg
     3601 aaattgaggt tagagaagac ggggaaaaag ggacttttgt acaattttta aagaatgatg
     3661 gattatatta tggagaaaat ttcagttgga atatttgggg ctttggagaa aatagctcag
     3721 gtaaagagtt taatttaaat attaaaacag atttaggtgt aggagctttt gtagatgaag
     3781 agaaatttat agtaagaagg agaacttggg gatatgactg ggtaaatgat tgggatgagc
     3841 agacatatac tttttattta ggtaaggaag atagaaattt attttgcata tatgaaaata
     3901 ataagatatt aacttcttta aagactgcta tagaagaaac tactcctaag atacaagttg
     3961 ccttaatgga tcataaaaat actataaaag catatctttc ccataagcct ttaattggag
     4021 ttaagtatgg attgtatata aacggaataa aggctaaggg agtttcaacc ctagttagag
     4081 aaaatgaaaa agaagtttta ataactaatc ttccaagtga tatagatcca tcagatttat
     4141 tagaagtaag agcttctagt atgttttcaa gttgcaaagt aatagtgaga gattatttaa
     4201 atgattatta ttatggagga aatgacctag gggtaagatt tagtgaagaa aatataagct
     4261 taaggttgtg ggcacctact gctaaaaaag tagaattatt aatatatgaa gattataaat
     4321 ctctaaggga aaatccttta agaaaatatg atatgaatag agaaaaagaa aatggaactc
     4381 atttaattaa agtaccaaga aaagaaaatg agggtaaata ttatttatat agattatact
     4441 ttaatggttt aagcaaaaaa ggtaaacacg taaataagat aacctatgct gtagaccctt
     4501 atgctgtttc tgtaggagta aatggagaaa agggggcttt agtagattta tttaataaag
     4561 agtgtgtgcc agaaggatgg aataaatata ataaacctaa gttaataaac aaagaagatt
     4621 caataattta tgagatgcat attagagatt ttaccataaa tgaaaattca ggagtttctg
     4681 aaagcttaag aggaaaattt ttagggactg ttgaagaggg aactttttat attgataaag
     4741 aaagtggaaa caaggttaaa acaggattag atcatttaaa ggaattaggg ataacccatg
     4801 ttcatttgct tccagtcttt gattttgcct ctgttaatga agaaataact aaggatgaaa
     4861 acaatagaaa ctggggatat gatcctaaaa actttaatgc catagaagga agttattcaa
     4921 cagacccata tacaccttca agaagaataa tagagtttag agaaatgata aaaaagtttc
     4981 atgataatgg aataagagtt atattagata tggtttataa tcatatgtat gaaactagta
     5041 atatggataa tatagttcct ttatattact ttagaagtga taaactagga aaatatacaa
     5101 atggatctgg ctgtggaaat gaaatggcct ctgaaaagcc tatggttagg aaatttattt
     5161 tagattccat attacattgg attaaaaatt atcatataga tggattaaga tttgacttaa
     5221 tggagcttat agatttagat actatgaagg aaatagtaaa aagaagtgaa gagattgatg
     5281 aaaaaatact tatttatggg gagccttgga aaggtggaga ttctccttta agtaatggaa
     5341 cttacaaggg aagtcaaaaa ggtcttggat tttccatatt taatgatgac tttagaaatg
     5401 ctctaagagg aaataatgac ccatctaatg gatttataaa tggagaacag cacaataaaa
     5461 ataaggtttg gcaagttata gaaggaatta agggatccat aaattcaata acctataaac
     5521 ctatggaaag tataaattat ttagaagcac atgataacta taccctatgg gatcaaatag
     5581 taaaaagtca aaatcatagt gtggaaaagg gacattatag agattttaat gaagaaaata
     5641 tattagataa tttttatgtt aaggaagatt tactaggagc ttctatatta tttacttctc
     5701 aaggaattcc ctttattcaa tctggagctg aatttttaag atctaaagat ggagatcata
     5761 atagctataa gagtccagac agtataaatg ctttaaattg ggcagagaaa gaaaagtata
     5821 ttgatgtttt taattattat aaagatttaa taagtttaag aaaaaatcac agtgctttta
     5881 gaatgaaaaa tccagaggat ataataggaa atttagaagt ttatttttat gacaataatg
     5941 atactagcgg agtaataata gctcactaca aaaataatgc taatgaagat ttatggaaag
     6001 atatagttgt catatataat ggaactacca ttgatgatta taatgtaatt tcttctatgc
     6061 ctaaatcaag taatggcttt tggaatattg cagttaaaag ttgggcttta aatcagtttg
     6121 gtatagaaag agtgagtgag gatgaaattc ccaaaataaa atctcattct atgatgatac
     6181 tttatgatga ataaattgaa gacttgtaaa gaggacaaat tagaaaattt aaggaagatg
     6241 aataatataa attatttgtt agaacaaaaa agaaataaaa aatgataaag ggagcgtgta
     6301 tcaaaataac ttttattata ttttgataca gcccctttta tcattaaaag attatttttc
     6361 aacttctcca tcccatttag atattccatc tttcatgtga acaagatttt taaaaccatt
     6421 ttcttttaaa atatttatag cctcagcact tttttttccg cttctacagt aaactaaaac
     6481 taattcatct ttaaactttt ctatatcatt taagttttcc ttaagttcct taactggaat
     6541 ttgaattgca ttttttaagt gtcctttagc aaattcatct gcatctctaa catctaagat
     6601 tagagtaacc tccccctggt ttataagttt tttagattca tctattgata tatcccttga
     6661 aacttcttca gattgaattt tacttgaaga ctcggagctt gtctgtgtat tagcatcctt
     6721 actttctgtg ttattactac cacatcctat aaataagcta gctgttaaag tcaaactaag
     6781 taataataat gatatttttt tcataaatat cctccctata ttaaaatata caaataaaat
     6841 ttaacatatt attacataat aaagcaataa aaaaagtgtt aatatttaag agcttaattt
     6901 aaggttattg acaattactt taataatctt tatagttaac tgtgtgaaat tatgtttata
     6961 attaaggaga ttaaaagata gatttttcta aaatggagat taacaatgaa aaaaaacaaa
     7021 ctatcaatta aaacaattgt agctatagga attggctcag cagtttttat gatattaggt
     7081 agatttggtt ctttgcctac aggaattcct aatacaaata tagaaacagc atatgctttt
     7141 ttatctttaa tggcattgtt atatggccct ttagctggtt ttttaatagg attcatagga
     7201 catgctttaa aagatatagt attctttgga tcaccttgga taagttgggt ttttgcttca
     7261 ggtatagtag gattaataat tggatttgga gctagattta taaaaattaa tcaaggggta
     7321 tttaagttaa agcaaatatt tatgtttaat ttaatacaga taattgctaa tggagtagct
     7381 tggtttttag tagcaccaac tttagatata ttaatttact ctgaaccttt aaataaggtt
     7441 tatctacaag gggtaattgg cggaatttct aatatgatta ctgttggagt tttaggaact
     7501 gttttaatat ctaactattc taagacaaga ataaaaaaag gtagcttaag aaaagaatat
     7561 tagaagtaca tgaggttgtg ggaaagatat tttcagacaa cctttttttg aagtttaatt
     7621 taggatatgt ataaagctta aacaaagagt taaaaaataa tatataaaat tccttaatta
     7681 aaaatgatta tttgaaagga aattgatatg gaaagaaaag caataataga gtttaaagat
     7741 tttactgctc attatacagt tcaatcaaag ccaactttaa ataacataaa tttaactata
     7801 tatgaaggag aaaaagttct tatagctggg ccttcagggt caggaaaaag tacattagct
     7861 aactgtataa atggattaat accattttca actgatattg aaatttcagg aagtcttaaa
     7921 ataaagggaa aagagacaaa ggatttaagt gtttttgaaa tatctaaaat ggtaggaact
     7981 gtacttcaag atccagatag tcaatttata ggattaactg tagctgagga tatagctttt
     8041 aaactagaaa ataactgtgt atctcaagaa gagatgaaga aaaaagttaa ttatgtatca
     8101 aaaatagttg atatagaaaa tagattagac ttagctccat atagtctttc tggtggacaa
     8161 aaacagaggg taaccttagc tggaactatt attgatgatg ttgatatact tctttttgat
     8221 gaacctttag caagcttaga tccagcggcg gggaaagctt ctatagaact tatagataaa
     8281 atacaaaagg aagaaaaaaa gacagtaata ataatagaac atagattaga agaagtttta
     8341 aattgtgatg tagacagaat aatacttatg aatgaaggag aaattattgc cgatacaact
     8401 ccaaatgaaa tattagcttc taataaatta aaagagtgtg gaataagaga acctttatac
     8461 ataacagctc ttaaatatag tggatatgaa ttaaataagg aaatgaacct tgagaatata
     8521 gacaggttaa agcttagtga tgatggcgag aaattaagaa cttggtataa gagtttaaat
     8581 tttaataaga aagaaagaga agaagacaca ctattagaat ttaaaaatgt atctttttct
     8641 tacgataaga aaaaagatat attaaatgat attaatttta aaattaatag aggagatatg
     8701 gtaagtgtag ttggaaaaaa tggcgctggt aaatcaacta tatctaagct catatgtggt
     8761 tttttcaaag aatcaaaggg agatataata tttgaaggga aaagtttaga tggaaagact
     8821 attaaggaaa gaggagaaga aataggagtt gttcttcaaa atccaaatca aatgatatct
     8881 aaaacaatga tctttgatga ggttgcatta ggattaagag ttagaggaat tgatgaaaaa
     8941 gaagttaagg aaagagtaat tgaaacttta aagacctgtg gtttatatcc atatagaaat
     9001 tggccagttt ctgcacttag ttttggacag aaaaagaggg ttactatagc ttcaatacta
     9061 gttttaaatc ctaaggttat aatacttgat gaaccaacag caggacaaga ctataaacat
     9121 tataatgaaa taatggagtt tcttctaaaa cttaataaaa agggagttac aataatttta
     9181 ataactcacg atatgcattt aatgcttgaa tacacagaca aggctattgt aatttcaaat
     9241 ggacgtaaaa tagctgataa aacatcagca gaaatactta cagataaaga agttatagaa
     9301 aaggcaaatt taaaagaaac atcattatat gatttagcct tgaaatttaa ggtagaaaac
     9361 cctaaggatt ttgtttgtaa atttattgat tttgacaggg gggtaagaaa ttagtggatg
     9421 caatgcttac atatataaaa aaggattctc ctattgataa acttactgga gcaactaagc
     9481 ttatttgctt tatattttgg acattagctg ccatgcttac atatgataca agggtattaa
     9541 ttggtatgct tatatttagt ttaattatat ttaaggtctc aaaaatagag tttaaagaag
     9601 tttcttttat agtttatttt atattattgt ttttactttt aaatgattta gccattttta
     9661 tattttcacc ttatgaagga gtcaatatat atggaacaag atatgatata tttcatataa
     9721 ttggcccata cacactaaca tgggaacaat tattttatga gtttaacgtt acattaaaat
     9781 atctatcagt tatacctatg gcacttttat ttatacttgc aacaaatcca agtgagtttg
     9841 ctgcatcttt aaataaaata ggtgttagtt acaaaatatc atactctgta tcaatagctt
     9901 taagatacat accagatatt caaagggagt atcatgatat atcctttgct caacaagcaa
     9961 gaggaattga tatgtctaaa aaagaaaagt taggaaaaag aataaaaaat atgtcatcca
    10021 ttttaatgcc acttattttt tcaagtttag atagaataga aaatataagt acagcgatgg
    10081 aacttagagc ctttggaaag aataaaaaaa gaacttggta caatgaaaga aaattttcta
    10141 agggtgatta cttagctatt attgtggtta tcttaatagt tgctttatca ctatacttta
    10201 catttaataa tggaagtaga ttttataatc cttttataaa atagtaaata aaaatataat
    10261 ttgaaag
//
LOCUS       DAEVGI010000087        10265 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEVGI010000087 DAEVGI010000000
VERSION     DAEVGI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.087"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            94..402
                     /locus_tag="K8M77_001719"
     CDS             94..402
                     /locus_tag="K8M77_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086211.1"
                     /translation="MEFLDWKFIFIIITFAFIGLICIFKKSKIGLTSASVGIIGSLIL
                     WGFFKVSIKVRNFLDGVGLSFKDLLNFLLVVITAIIAFLVIFIFLKAFNNFGSKISKR
                     "
     gene            complement(422..1654)
                     /locus_tag="K8M77_001720"
     CDS             complement(422..1654)
                     /locus_tag="K8M77_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086212.1"
                     /translation="MQKRFLSALIAMSISISATHVVFADTVNDKKSTIQENKVKYSQL
                     DNEVISLNSQVLKLNNEIEDLNAKLEDNKAKMKDTEENLKETESKVSTLKTEINEKQS
                     VLGKRMRAMYKSKDSMNPVVFLLKSENLSDLITRIDALARVTALDKNLIQSLDEQKDS
                     LNSDIKKLERDKAELKELKASNEESLKTLDNKKIEEQKKIDELNKQKEAVLEVIKENE
                     MSLISHSVSIINSSSSINELESAVSTLNQLIPQLNIDSVKEAANNSLQAAKNKIESLK
                     AEEAKKAEEAAKNNAANSSNPTSSNNSYSQPSSDGKYKKTLSMEATAYSGGTLTAMGL
                     KPVRDPGGISTIAVDPSVIPLGSKVYIPGYGYAIASDTGGVIKGNIIDLYMNSHDECT
                     SWGRRQVTLHIVAYPGEW"
     regulatory      1886..2065
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2187..3029
                     /locus_tag="K8M77_001721"
     CDS             2187..3029
                     /locus_tag="K8M77_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HBI7086213.1"
                     /translation="MKKAILVVSFGTSHLDALNNSIKKIEDSIQENFKEYDIFRSFTS
                     HIIVRKLKEKHNMEVLFPEDALKKLGEEGYEEVIVQPLHIIPGEEFDYIKHMVKEYEP
                     CFKKIEIGRPIFHYEGFESAPDDYSYFIDTMKDLIENNSPCVFVGHGSAHPSNAVYGC
                     LQSVLIDKDYEDVFVGTVEGYPNFSNVLKKAKKKDIKEITIIPLMVVAGDHAKNDMAS
                     EEEDSWKSRFESEGIKANVILKGLGEYEEFGNLYIDRINDVINGTYKGLGETKKKKHR
                     REMK"
     gene            3029..3658
                     /locus_tag="K8M77_001722"
     CDS             3029..3658
                     /locus_tag="K8M77_001722"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HBI7086214.1"
                     /translation="MDYMKSPMGIEKRSFEIIGSEMHDHNFSEEELQIVKRVIHTTAD
                     FEYQDLIEISEGAIEAGVSALKNGAKIYTDTTMALSGMNKRALKELNASVECYVGLEE
                     VFKLAKEKEITRSMAGVELAAKENVDIFVFGNAPTALFKLRELIKEGKANPKLIVAVP
                     VGFVGASESKEDLDELGIPYIRVKGRKGGSTVAAAIINALLYMNYKRDM"
     gene            3676..4773
                     /gene="cbiD"
                     /locus_tag="K8M77_001723"
     CDS             3676..4773
                     /gene="cbiD"
                     /locus_tag="K8M77_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiD"
                     /protein_id="HBI7086215.1"
                     /translation="MFDMYIESGGKKLRCGYTTGSCAAAAAKAATYMLFNKKDISVIE
                     IDTPKNIKLNLEIQDIQVEKNSISCSIVKDGGDDIDATSGLEIFAKAEEIEEGFELCG
                     GEGVGVVTKEGLFVEKGQPAINPVPREMIKKEVLSVLPKDKGVRITIFVPRGREIAKK
                     TFNPRLGIVNGISILGTTGIVYPMSEEALKESIRIEIRQKAVNNKDLVFVFGNMGERF
                     LRERGYKKDNIVVISNYVGFSIECALAQGIKDLTIVGHIGKLSKIAFGCFNTHSRVSD
                     VRLEVIALELTLMGYDLELVQKVLDQKTSEGAVRLLGDDFPMLYERIGEKVLKRLDIY
                     AYGEANFNILMYYGSKEMKLLYESKNSNLFD"
     gene            4801..5412
                     /gene="cbiE"
                     /locus_tag="K8M77_001724"
     CDS             4801..5412
                     /gene="cbiE"
                     /locus_tag="K8M77_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HBI7086216.1"
                     /translation="MVKILGLGPGHEDYIIPKVIKELEKSDYIVGFERAISSLKFLNS
                     INDERFKRVKGLKEIIEFLNENKEKRISIVASGDPTFYGITNYICKNYEGEVSIVPGI
                     SSFQYLTCRVNKTWNDAFVGSMHGREENFIEVVKENKLSIWLTDKKNTPSELSKRLIE
                     NSIECTIYVGENLSYEDENITIGNPREICEKDYGDLSVIIIER"
     gene            5432..6025
                     /gene="cbiT"
                     /locus_tag="K8M77_001725"
     CDS             5432..6025
                     /gene="cbiT"
                     /locus_tag="K8M77_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HBI7086217.1"
                     /translation="MKFIKDSEFIRGKCPMTKEDIRILTISKLDLDKDSNFLDIGSGT
                     GSITIQASKFIEEGAVFSIERDEEAIRVTKENLKKFNCNNVTLLEGDAIEHMESLSKE
                     IKKEKISKFDSIFIGGSGGGLEKIINISDEILKEHGVLVLNFITLNNAYNAIEFLKKL
                     EYKTEISLVNISKNRENTYMMISNNPIFIVKAFKEEN"
     gene            6025..6687
                     /locus_tag="K8M77_001726"
     CDS             6025..6687
                     /locus_tag="K8M77_001726"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBI7086218.1"
                     /translation="MAILYGIGVGPGDSELLTIKAVKAIENSDVIVAPSAEEGGESIA
                     LIAAQEYIKEGTEIAVKHFPMGKKNRELKVKEAYDFILEKLKEGKNVSFLTIGDPYVY
                     STYIHLLNYVEDRGFHVKTIPGITSFCAAASIVDRTLVVGNEPLLVLPAGRVHDIKDE
                     KFVVIMKVYKKEEEVLDVLEEKGFDYVLVSRAGREGEKVLRTREEILNHRDYMSLILA
                     SK"
     gene            6738..7493
                     /gene="cobM"
                     /locus_tag="K8M77_001727"
     CDS             6738..7493
                     /gene="cobM"
                     /locus_tag="K8M77_001727"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HBI7086219.1"
                     /translation="MLYFIGAGPGAVDLITVRGRNLLEKADVVIYAGSLVSKEHLNVC
                     KEGVKIYNSASMTLEEVVEVIKENDGEDKVIVRLHTGDPSIYGAIKEQMDELDKFNIK
                     YEVVPGVSSFTAAASAIKKEFTLPSVTQTVILTRVEGRTPVPETEDLEKLAGIGASMA
                     LFLSISMIDKVVEKLKRGYGREDVPIAVISRATWDDQEIIIGTLENISEKVKEAGINK
                     CAQILVGDFIDCNYEKSLLYDKHFTHMFRKGIE"
     gene            7496..8494
                     /gene="cbiG"
                     /locus_tag="K8M77_001728"
     CDS             7496..8494
                     /gene="cbiG"
                     /locus_tag="K8M77_001728"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBI7086220.1"
                     /translation="MIGIISVTEKGDFIGDKLKDFFFENNISFKGIYKSKCEDFSLKG
                     ATQEMFETCKSIIFISSTGIAVRAITPFIVKKDKDPGIVVVDVNNKFTISLAGGHIGG
                     ANELTLEISKVLNNTPVITTATDNQNKVAPDMIAKSNNLIIEDLKKAKDMASRLVNNK
                     KVYFLDDDNLIKLPLGYESTYLLKDNTLWITNKEKSEEENLNGVLRLIRKNILLGVGC
                     RKGVSSQGLIDFVKSSLKDLNIDKRAVLKIGSIDLKKEEKAILDLSEYLNCHFETFSK
                     DEIKSVQDEFEGSDFVEKSVGVRAVSEPAVLLMNGNIIVNKLKKNGMTLTVGELKR"
     gene            8649..9371
                     /gene="cobJ"
                     /locus_tag="K8M77_001729"
     CDS             8649..9371
                     /gene="cobJ"
                     /locus_tag="K8M77_001729"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBI7086221.1"
                     /translation="MGKLYVIGIGPGGLDEMTLRAVKAIEESDIIVGYTKYIEMVKDL
                     IKDKEIFKTGMRGEEERCREALELSKDKKVALISTGDSGIYGMAGLILEMRKDENVEI
                     IPGITASSAAGSVLGAPLMHDNCNISLSDLMTPYEDIKKRVRLAAEGDFVISLYNPKS
                     KGRPHYLRECVDIIKEFRGEETPIAVVRNALREGESKEIFTLKDFNDEVVDMFSIVII
                     GNSKSYIKDGYFVTPRGYKIKK"
     gene            9390..10154
                     /locus_tag="K8M77_001730"
     CDS             9390..10154
                     /locus_tag="K8M77_001730"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HBI7086222.1"
                     /translation="MIALILGTSEGREFLQKLKGFEEKIVISTATEYGGELLKEFPVA
                     FMNTKPLDEEGLLNFAKEFNLKGIVDASHPYAKNVSENTIKVCKKLNIEYIRYERNGV
                     LKELVNPNIIRISGLEKLKELDSTINGPILNTTGSNGVKQILESSLENRVIHRVLPSK
                     KVMNDLIDLGVKIEDIIAIKGPIGYELNKAFIDEYKAKALLTKDSGERGGALEKAKAA
                     LDSNIKLIIVEKPKIDYGRCFSDIDEIVKYVKNILK"
BASE COUNT         4024 a         1070 c         1891 g         3280 t
ORIGIN      
        1 ttaataaaat aattaaaatc ttaaataagt aaatgaataa ttaaaacttt tattaagtaa
       61 aataatagag aagtattttt aggagaatag attttggaat ttttagattg gaaatttatt
      121 tttataataa taacttttgc ttttataggc ctaatatgta tatttaaaaa atcaaagatt
      181 ggattaacat cagcctcagt aggtattatt ggaagtttaa ttttatgggg attttttaag
      241 gtaagtatta aggttagaaa tttcttagat ggagtaggtt taagttttaa agatctatta
      301 aactttttat tggtagttat tactgcaata atagcatttt tagtaatttt tattttttta
      361 aaggcattta ataattttgg aagtaaaata agcaaaagat aagaccaaaa gtcttatctt
      421 tttaccattc accaggataa gcaactatgt gtaatgtaac ttgacgtctt ccccaagatg
      481 tacattcatc atgagagttc atataaaggt cgataatatt tccttttata actccacctg
      541 tatctgatgc tatagcataa ccataaccag ggatgtacac ttttgatcct aaaggaatta
      601 cactagggtc aacagctatt gtacttattc cacctggatc tcttacaggt ttaagtccca
      661 tagctgttaa ggttccacca ctatatgcag tggcttccat agaaagtgtt ttcttatact
      721 tgccatcgct actaggttgg ctataactat tattactgct agtaggattt gaagagtttg
      781 cagcattatt tttagcggct tcctctgctt ttttagcttc ttcagctttt aatgattcaa
      841 ttttattttt agcagcttgt aaagaattgt tagctgcctc ttttacagaa tcaatgttaa
      901 gttgtggtat taattgattc aatgtgctta ctgcactttc aagttcatta attgatgaac
      961 ttgaatttat aattgaaact gaatgagata ttaaagacat ttcattttct ttaatcactt
     1021 ctaaaacagc ttctttttgt ttgtttaatt catcaatttt cttttgttct tcaatttttt
     1081 tattatctaa ggttttaaga gattcctcat ttgaagcttt caactcttta agctcagctt
     1141 tatctctctc taacttttta atatcactat taagagaatc tttttgctca tctaaacttt
     1201 gtataagatt tttatctaaa gctgtaaccc ttgccaaagc atctattctt gttattaaat
     1261 cagataagtt ttcagattta agcaagaaaa ctacgggatt catagaatcc ttacttttat
     1321 acatagcacg cattcttttt cctaaaacag attgcttttc atttatttca gtttttaaag
     1381 tgcttacttt gctttctgtc tcttttaaat tctcttctgt atctttcatt ttagccttat
     1441 tatcttctaa tttggcattt aaatcttcaa tttcattatt aagttttaac acttgagagt
     1501 taagtgaaat aacttcatta tctaattgtg aatattttac tttattctcc tgtatagtag
     1561 atttcttatc atttactgta tcagcaaaaa ctacatgagt agctgaaatg ctaattgaca
     1621 tagcaattaa tgctgacaaa aatctttttt gcataaacta cactccttta gttaactgtc
     1681 cacaacttaa gtgtatttca tcatatatta ttgaattata acataaaagt caaatgtaac
     1741 acaaatttaa cctttaaata tttaaaatat ggtaagaaaa aaattaacaa tttaattata
     1801 ctacaaattt aaaaaaataa gaaatttacc ttataattta ccttatattg aaataattaa
     1861 aattatatat tataattaaa aaaaataata ttttatattt ttaggtttga ttttaattaa
     1921 aagggaaagt ggttaaaatc cactacagcc cccgctactg tgataggata caagtttcta
     1981 tttgaccact gattatataa attgggaagg gagaaatgag gataagcctt aagtcaggat
     2041 acctgcctaa agatcatgaa ctaagcttcg gagggaagag attagtactt atttgttcat
     2101 aattttgagg aataattact ttgatttgta agcatctgtt ctttgtagca cagatgcttt
     2161 ttatttttaa attttagagg tgaaacatga aaaaagctat attagtagta agttttggaa
     2221 caagtcattt agacgcgtta aataattcta taaaaaaaat agaagattca atacaagaaa
     2281 attttaaaga atatgatatt tttagaagtt ttacatcaca tataatagta agaaaactta
     2341 aagaaaaaca taacatggaa gtattatttc cagaggatgc tttaaaaaaa ttaggagagg
     2401 aaggatatga agaagttata gttcaaccac ttcatattat tccaggagaa gaatttgatt
     2461 atataaagca tatggtaaag gaatatgaac catgttttaa aaagatagaa ataggaagac
     2521 caatattcca ttatgaaggt tttgaatcag ctccagatga ttattcatat ttcatagata
     2581 caatgaaaga tttaatagaa aataattctc catgtgtatt tgttggacat ggtagtgccc
     2641 atccatcaaa tgctgtttat ggatgtttac aatctgtttt aatagataag gactatgaag
     2701 atgtttttgt aggaactgta gaagggtatc ctaatttttc aaatgtctta aagaaagcta
     2761 agaagaaaga tataaaagaa ataacaataa ttcctttaat ggttgtagct ggagatcatg
     2821 ctaaaaatga tatggcttca gaggaagaag attcatggaa gagcagattt gaaagtgaag
     2881 gaataaaggc taatgttatt ttaaaaggct taggagaata cgaagagttt ggaaatttat
     2941 acatagatag aattaatgat gttattaatg gtacatataa aggacttgga gaaactaaaa
     3001 agaagaaaca tagaagggaa atgaaataat ggattacatg aaaagcccta tgggaattga
     3061 aaaaagaagt tttgaaatta taggttctga aatgcatgat cataattttt cagaagaaga
     3121 attacaaata gtaaaaagag ttatacatac tactgcggat tttgaatatc aagatttaat
     3181 agaaataagt gaaggtgcta tagaggcagg agtttctgca ttaaagaatg gagctaaaat
     3241 atatactgat acaacaatgg ctttatcagg aatgaacaaa agagcattaa aagaattaaa
     3301 tgcatcagtt gaatgctatg ttggattaga agaggtattt aaattagcta aagaaaaaga
     3361 aataactaga tcaatggcag gagttgaact tgcagcaaag gaaaatgtag atatatttgt
     3421 ttttgggaac gctccaactg ccttatttaa acttagagaa cttattaagg aaggaaaagc
     3481 taatccaaag cttatagtag cagttccagt tggttttgta ggggctagtg agagtaaaga
     3541 agatttagat gaacttggaa ttccttatat aagagttaag ggaagaaaag gtggaagtac
     3601 agtagcagca gctataataa atgcattact ttatatgaat tataaaagag atatgtaata
     3661 aagaaggtaa tctaaatgtt tgatatgtat atagaaagcg gtggaaagaa acttagatgt
     3721 ggatacacta cagggtcttg tgcagcagct gctgctaaag ccgccactta tatgcttttt
     3781 aataaaaaag acataagtgt tatagaaata gatactccta aaaatataaa acttaattta
     3841 gaaatacaag atatacaggt cgaaaaaaat tcaatctctt gctccatagt aaaagatggg
     3901 ggagatgaca tagatgctac ctcaggtctt gaaatctttg caaaggcaga agaaattgaa
     3961 gaaggctttg aactatgtgg aggagaaggc gttggagtag taactaaaga ggggcttttt
     4021 gtagaaaaag gacaacctgc cataaatcca gtacctagag aaatgataaa aaaagaagtc
     4081 ttaagtgtac ttcctaagga taagggagtt agaataacaa ttttcgttcc aagaggaaga
     4141 gagattgcta agaaaacatt taaccctaga ttaggtatag ttaatggaat atcaatactt
     4201 ggtactaccg gaatagttta tccaatgtca gaagaggcct taaaagaatc tattaggatt
     4261 gaaataaggc aaaaagccgt aaataataaa gaccttgtat ttgtatttgg aaatatggga
     4321 gaaagatttt taagagaaag aggatacaaa aaagacaata tagttgttat atctaattat
     4381 gtaggttttt ctatagagtg tgccttagct caaggaataa aggatttaac catagtaggc
     4441 catataggaa agttaagcaa gattgccttt ggatgtttca atacacatag tagggtttca
     4501 gatgttagat tagaagttat agccctagag cttacattaa tgggatatga tttagaactg
     4561 gtacaaaaag ttttagatca aaaaacaagt gaaggtgccg taagacttct tggagatgat
     4621 tttcctatgt tatatgaaag aattggagaa aaggtattaa agagattaga tatatatgct
     4681 tatggtgaag caaactttaa tattcttatg tattatggat caaaggaaat gaagttacta
     4741 tatgaatcta aaaattcaaa cttgtttgat taaacttaaa ttagaaagga atgaaggctt
     4801 ttggttaaaa tattaggatt aggaccaggc catgaagact atataattcc taaagttatt
     4861 aaggaactag aaaaaagtga ttatatagtt ggttttgaaa gagcaatttc tagcttaaag
     4921 tttttaaata gtataaatga tgaaagattt aagagagtaa aaggtttaaa agaaataata
     4981 gaatttctaa atgaaaataa ggaaaagagg atttcaatag ttgcctcagg agatccaacc
     5041 ttttatggaa ttacaaatta tatatgtaag aattatgagg gagaagtttc tattgttcct
     5101 ggaataagtt cttttcaata tttaacatgc agagttaata agacttggaa tgatgctttt
     5161 gttggaagta tgcatggaag agaagaaaac tttatagaag ttgtgaagga aaacaaactt
     5221 tctatttggt taacagataa aaaaaatact ccttctgagc taagtaaaag gttaatagaa
     5281 aattctatag aatgtactat atatgttgga gaaaatcttt cttatgaaga tgaaaatata
     5341 acaattggaa atccaagaga aatttgcgaa aaggattatg gagatttatc tgtaataata
     5401 attgaaagat aaattttcct ttggaggata tatgaagttt ataaaagatt cagagtttat
     5461 aagaggaaaa tgtcctatga caaaagagga tattagaatc cttactatta gtaaattaga
     5521 tttagataaa gattctaatt ttttagatat aggaagtgga actggtagta ttactattca
     5581 agcttctaaa tttatagaag agggagctgt attttctata gaaagagatg aagaagccat
     5641 aagagtaact aaggagaact taaaaaaatt taattgtaat aatgttactc ttttagaggg
     5701 tgatgctata gaacatatgg aatcactttc aaaggaaatt aaaaaagaaa aaatctcaaa
     5761 atttgattct atatttatag gaggctctgg tggaggcctt gaaaaaatta taaatatatc
     5821 agatgaaatc ttaaaagagc atggagtttt agtattaaac tttataactt taaacaatgc
     5881 atataatgca atagaatttt taaagaaatt agaatataaa acagaaattt ctttagttaa
     5941 cataagtaaa aatagagaaa atacatacat gatgattagc aataatccaa tttttattgt
     6001 taaggcattt aaggaggaaa attaatggca atattatatg gaataggtgt aggaccagga
     6061 gatagtgaac tattaacaat aaaggcagta aaggctattg aaaatagtga tgttatagta
     6121 gcaccaagtg cagaagaagg tggagagagt atagcattaa tagcagctca ggaatacata
     6181 aaagaaggaa cagaaatagc tgttaaacat ttcccaatgg ggaagaaaaa cagagaatta
     6241 aaagttaaag aggcttatga ctttatttta gaaaaactta aagaaggaaa aaatgtaagt
     6301 ttcttaacta taggtgatcc ttatgtttat agtacttata tacatttatt aaattatgta
     6361 gaagatcgtg gttttcatgt aaaaactatt ccaggaataa catctttctg tgctgcagct
     6421 agcatagttg atagaacatt agttgtaggt aatgaaccat tacttgttct tccagcaggt
     6481 agagtacatg acataaaaga tgaaaagttt gttgtaataa tgaaagttta taaaaaagaa
     6541 gaagaagttt tagatgtctt agaagaaaaa ggatttgact atgtgctagt tagtagagca
     6601 ggtagagaag gggaaaaagt tctaagaact agagaagaaa tcttaaatca tagagattac
     6661 atgtctttaa tactagcaag taaatagaga aaaattataa ataaaaaatc gtattttatt
     6721 aggaggcaag ggataaaatg ttatatttta taggtgcagg tccaggagct gttgatttaa
     6781 taactgttag aggaagaaat ttattagaaa aagcagatgt tgtaatatat gcaggatctt
     6841 tagtaagcaa agaacattta aatgtttgta aagaaggagt taaaatatat aactcagctt
     6901 caatgacttt agaagaggta gttgaagtta taaaagaaaa tgatggtgaa gataaagtaa
     6961 tagtaagact tcacactgga gatccatcaa tatatggagc tattaaggaa caaatggatg
     7021 aactagataa gttcaatata aaatatgagg ttgtacctgg agtaagttca tttacagcag
     7081 cagcttcagc cataaagaaa gagtttactc ttccatcagt aactcaaact gttatattaa
     7141 ctagagttga aggaagaaca ccagtaccag agacagaaga tttagaaaag ttagcaggaa
     7201 ttggagcttc tatggcatta ttcctttcta tatcaatgat tgataaggta gttgaaaaat
     7261 taaaaagagg atatggaaga gaagatgttc ctatagctgt aatttctaga gcaacatggg
     7321 atgatcaaga aataatcata ggaacactag aaaatatatc agaaaaggtt aaggaagctg
     7381 gtataaataa atgtgctcaa atattagttg gagatttcat tgactgtaat tatgagaaga
     7441 gtcttcttta tgataagcat tttactcata tgttcagaaa aggaattgaa taaatatgat
     7501 tggaataata agtgttactg aaaaaggaga ctttataggg gataagctta aggatttttt
     7561 ctttgaaaat aatattagtt tcaaaggaat ttataaaagt aaatgtgaag atttttcttt
     7621 aaaaggagcc acacaggaaa tgtttgaaac ttgtaagagt attattttta taagttcaac
     7681 aggaattgca gttagggcta taactccttt tatagtaaaa aaagataagg atccaggaat
     7741 tgttgtggtt gatgtaaata ataagtttac tataagttta gcaggaggac acattggtgg
     7801 agccaatgaa ttaactttag aaataagcaa agttttaaat aatactccag ttataacaac
     7861 tgccacagat aaccaaaata aagttgcacc agacatgatt gctaaaagta ataatcttat
     7921 tatagaagat ttaaaaaagg ctaaagacat ggcttcaagg ttagtaaaca ataaaaaagt
     7981 ttatttttta gatgatgata atttaattaa attgccttta ggctatgaaa gtacatattt
     8041 attaaaagac aatactttat ggataactaa taaggaaaaa agtgaagaag aaaatcttaa
     8101 tggagtttta agattaataa gaaaaaatat attacttgga gtaggttgcc gaaaaggagt
     8161 gtcttcacaa gggttaatag attttgtaaa atcctcactt aaggatttaa atattgataa
     8221 gagagctgtc ttgaaaatag gatcaataga tttgaaaaaa gaggaaaagg ctattttaga
     8281 tttaagtgaa tatttaaatt gtcattttga aaccttttct aaggatgaga ttaagagtgt
     8341 tcaagatgaa tttgaaggta gtgattttgt agaaaaatct gttggagtaa gagctgtatc
     8401 agagccagca gttcttttaa tgaatggaaa tattatagta aataaactaa agaaaaatgg
     8461 aatgacgtta actgttggag agcttaaaag gtaaagaaaa ggaaagtaag ctttataaat
     8521 gtagaaaaaa ctctattaag ataagtgtct tagaaattct ttaaaagtat agtagaaaag
     8581 taaaagtttt aataagctta taaccataat actttacaac ttaagccaca taataaggag
     8641 gaataagaat gggaaaatta tatgtaattg gaattggacc aggtggatta gatgaaatga
     8701 ctttaagagc agttaaagct attgaagaat ctgacataat agttgggtac actaaatata
     8761 tagaaatggt taaagactta ataaaagata aggaaatctt taaaactggt atgcgtggag
     8821 aagaagaaag atgcagagaa gccttagaac ttagtaagga taaaaaagtt gctttaataa
     8881 gtactggaga ttcaggaatt tatggtatgg ctggacttat cttagagatg agaaaagatg
     8941 aaaatgttga aataatacct ggtattacag catctagtgc agcaggatca gttttaggtg
     9001 ctcctttaat gcatgataac tgtaacataa gtttaagtga tttaatgact ccttatgaag
     9061 atataaagaa aagagttcgt ttagcagcag agggagattt cgtaatttct ctttacaatc
     9121 caaagagtaa aggaagacca cactatttaa gagagtgtgt tgatataatt aaggaattta
     9181 gaggagaaga aactcctata gcagttgtta gaaatgcctt aagagaagga gaaagtaaag
     9241 aaatatttac tttaaaagac tttaatgatg aagtagttga tatgttctca atagtaatca
     9301 taggaaactc aaaaagttat attaaagatg gatattttgt aactccaaga ggatataaaa
     9361 taaaaaaata aaactaatgg ggtgagaatg tgattgcact tatattagga acttcagaag
     9421 gaagagagtt tttacaaaag cttaagggat ttgaagaaaa aatagttata tctacagcta
     9481 cagaatatgg tggggaactt ttaaaagaat tccctgttgc atttatgaat acaaaacctt
     9541 tagatgaaga aggactttta aattttgcta aagaatttaa tttaaaagga atagttgatg
     9601 cttctcatcc ttatgcaaag aatgttagtg aaaacacaat aaaagtatgt aagaaattaa
     9661 acatagaata cataagatat gaaagaaatg gtgtactaaa agagttagtc aatcctaata
     9721 ttattagaat aagtggatta gagaaactta aagaattaga ctctactatt aatggaccaa
     9781 ttttaaacac cacaggaagt aatggagtaa agcaaatatt agaatcttct cttgaaaata
     9841 gagttattca tagagtatta ccttcaaaaa aggttatgaa tgatcttata gatttagggg
     9901 ttaagataga agatataatt gctataaaag gtcctattgg ctatgaactt aacaaggctt
     9961 ttatagatga atacaaggca aaggctcttt taactaaaga tagtggtgaa agaggaggag
    10021 ctttagaaaa agcaaaggct gcattagatt caaatattaa attaataata gttgaaaagc
    10081 ctaagataga ttatggtaga tgtttttctg atatagatga aatagtaaaa tacgttaaaa
    10141 atattttgaa ataataagaa tcttaaatga aataaaacaa ctttatggta aaattatgaa
    10201 aagataaaga aattttaaga atagttaact ttttatttta gatagtgact ctgttttaag
    10261 cttat
//
LOCUS       DAEVGI010000088        10162 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEVGI010000088 DAEVGI010000000
VERSION     DAEVGI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.088"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(204..704)
                     /locus_tag="K8M77_001731"
     CDS             complement(204..704)
                     /locus_tag="K8M77_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBI7086223.1"
                     /translation="MSLYKQWTDMVVEYVKVKGEAAFWKEYSDVETKIYKDLLANHKE
                     VKKATIADLAKEYGTTEEFVMGFVDGINDSLKTPYDLEKVESNTELSFDIDFENLYYN
                     MLDAKADYLFNLPQWDAIFSGEKRREIQFRYRDSKTVRNEEVKVGRNEPCPCGSGKKY
                     KKCCGK"
     gene            complement(847..1401)
                     /locus_tag="K8M77_001732"
     CDS             complement(847..1401)
                     /locus_tag="K8M77_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBI7086224.1"
                     /translation="MKKGMILLIISSFLLVGCDSIKMDKLAANNNERVKTIANDLIGD
                     IDLSLYFDGTKDEQNPKIEQQEILVDGDEILGQYLIQALIQGPSQKGSLAPILPKDTK
                     LLSFDIKDGIAIINLSKEAIINMSATKEQATLEGIIATITQIPNINKINILVDNQMVD
                     SLGGNFDISKPFGKEDIPNLKINN"
     gene            complement(1417..2208)
                     /locus_tag="K8M77_001733"
     CDS             complement(1417..2208)
                     /locus_tag="K8M77_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI7086225.1"
                     /translation="MIFCSLYSGSSGNSILVGSEKTKILIDAGLSGKKIIDGLQEVGQ
                     NPNEIDGIFITHEHSDHIKGAGILSRKFNIPIYANELTWKAMEKSLGKIKEENIKIMP
                     KRSTMSLNDLDITSFNTPHDSVASTGYTIKHKGKSASIATDIGTFTEEILRNIRESEV
                     ILLESNHDVQMVKFGPYPYELKRRVLSEVGHLSNEDCGKAIVDIMNHDKHRKIILGHL
                     SNTNNVPELAEQAVINVLSANKINIGKDLELKLADRHKPSSYISF"
     gene            complement(2231..3484)
                     /locus_tag="K8M77_001734"
     CDS             complement(2231..3484)
                     /locus_tag="K8M77_001734"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBI7086226.1"
                     /translation="MNRLIINGGKALKGSIEINGAKNAAVAILPAAILASKGECIIDN
                     VPDIADVHCLERIIRSLGCNVEKLDNNTLKINAEEIKTVEACGNDVRKMRASYYFIGA
                     LLARFKEAKVELPGGCPIGVRPIDQHIKGFEALGAKVSIEHGAVNIMAEKLIGTNIFF
                     DVVSVGATINLMIAATLAEGTTVLENAAREPHVVDVANFLNAMGANVKGAGTDVIRIK
                     GVKELKGCNYSVVPDQIEAGTFMIAAAATRGDVTIQNVIPKHLESISAKLLEMGAKVE
                     EGDDSVRVYVDGDLKGVNLKTAPYPGFPTDVQQPMCTLLSTAKGRSIIVETIWENRHK
                     HVDELKKMGATIKVEGRSAIIDGVDRLTGAVVKATDLRAGAAMVIAGLISDGVTEITS
                     IEHIDRGYPHIEEKFRMLGADIVRK"
     gene            3824..4369
                     /locus_tag="K8M77_001735"
     CDS             3824..4369
                     /locus_tag="K8M77_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086227.1"
                     /translation="MKYYLVALFDEESCKFLESIQRGLLRKYKLPRNHISLHIPLETI
                     DNPNLDKLDEVVLKLIKPYKKFKIELTGSTEFNESTNKALNLQIENKGYIKKLHRLFN
                     DMLKLHGFNVRERVDSPLYIPINPPNFKENHKKNNTQPTVFSLRESSQKNILKVSKIE
                     LWRFQNNKREIPVKSYDLRNY"
     gene            4563..5042
                     /locus_tag="K8M77_001736"
     CDS             4563..5042
                     /locus_tag="K8M77_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBI7086228.1"
                     /translation="MELDNFFIQQDKFNKELVIDSNLNDYKVATRMYLALQVKISELA
                     DETKCFIYWNDSNKSRVSKQLVLEKYIDCLKQILTIGLNKNYTCLKKLIVKPNDYCLS
                     DQFLTLLIDVNDLIISPSIDHYKTLFEDFISLGISLGFSINQIEEIFFKNDCCKIAL"
     gene            5098..5529
                     /locus_tag="K8M77_001737"
     CDS             5098..5529
                     /locus_tag="K8M77_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI7086229.1"
                     /translation="MLGIILAIISGAAMSIQGVFNTRVSEKIGVWETNTIVQGSAFVL
                     ALFIMLMLRNGNYSELRSVNKIYLLGGAIGVVITYTVMAAVGSLGPTFGISIILISQL
                     LTAGIIDTFALFDSEKIRFAAHNYLGIILMIVGIIIFKWKS"
     gene            5596..6792
                     /locus_tag="K8M77_001738"
     CDS             5596..6792
                     /locus_tag="K8M77_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBI7086230.1"
                     /translation="MFSIKQKLDSNLKVYINRSYYTNYRVLIKCKKFMEDITKKIPKL
                     RGIVIREIKSLNLICAILTPKAINRLIEYPEIEFISFDDHAILCGLSIGTANRIATNK
                     SFNFTGKNVSIGLIDSGVYPHQDLTNPTNKIDMFLDLLNNYSYPYDDNGHGTALSGII
                     CGSGYSSKLVFRGIAENTKISCVKAFDANGKGYVSDILFAIETLINQENNPIRVLCLP
                     FELTSHNIKISDYFNELFKLAVSKNIIPVVPSGSIEGDNTIQGLALSPWCITVGGIDS
                     TKTPTTTFKFSSSGNSNVKKPDFCAACANIMCLNSDKKYISERNGIKLYPHKLDSSYT
                     VFQGTSLACAYISGVCALLLEAKPELNYKDLCSLLKIASNNKYELPSDSVGEGVIDLS
                     FLLENI"
     gene            complement(6795..7847)
                     /locus_tag="K8M77_001739"
     CDS             complement(6795..7847)
                     /locus_tag="K8M77_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI7086231.1"
                     /translation="MSISEGEGRNSIKVFLITLIILFCTVKTNVFANSLYPRMGSGKY
                     IFVNMPCIANVYVEAYELSLNNKVIGYIGNPDTVETVLNKIKQTYIDKASEKGVLVKE
                     VNFDTKIDLKKNMIQSKDLSSVEDITSKIMLANDILEESLLDVTIEAEINEIASIEPA
                     VEVIKLEDKYLGENTIEEGKLGEKKVKKKVILVNGKESESEIISESVLKEAESTKIYK
                     GIKNPINSNMAFLAHPTRGGVITSVFGEKSRGGHRGVDIAVPSGTPIGAACDGVVSFV
                     GYDDIYGNMVKIKHDDNTETLYAHASYILTEIGKEVKKGETIAKVGSTGRSTGPHLHL
                     ELIYKGNPINPVDYIS"
     gene            8078..9079
                     /locus_tag="K8M77_001740"
     CDS             8078..9079
                     /locus_tag="K8M77_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBI7086232.1"
                     /translation="MSTFMLKNSATKFTPVSNVFLEEYMPKARGEFIKVYLLLLKYNF
                     TGEPGVNSSILATNLNLLESDIMNALNYWHDEGVIKLVPIDKMNNFKIEFLDLSEGNH
                     SSSQNSVDLLSALDENNTKDMLKDIEKLICRPLSTKEMTTYLSWQTELNFSSELILIL
                     IEYCVSKGKKDVRYIETVALGWHNAGIKSIEEAQAYITKTEDKWVKIRKILKYLGIQN
                     TEIMKPQETMMEKWLLTYNFSVELILKACDICFERLNRADFKYIDGILGKWFKDGIKT
                     LDDVAKKDIKKTSSIKKPFNKNNSAPIQKKPAREANFTQRDYDYDSLEKQLLGWDIE"
     gene            9076..10071
                     /locus_tag="K8M77_001741"
     CDS             9076..10071
                     /locus_tag="K8M77_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI7086233.1"
                     /translation="MIKGYKSQLMDMYEKIRQKEKSNLANRQKELKEKLPQVFEYERE
                     INKLSLKLSMLALRKHEKESFEKLKKEIENLRDEKDQLLAMSGYPKDYLDMHYICPSC
                     KDTGYIGTKKCICYKKKLVDIYYENSHMADALKLNNFSKFDINVFSPLNNGNEKYSPR
                     ENMQIIIDKMVNGYIPKFDTHNENILFYGSPGTGKTFLTYCIAKELLDNGFLVVYRTS
                     DELIRNLREIRFNNDHELEDLLINCDLLVIDDLGAEQITDFSTTELFTLLNKKILLNK
                     KMIISTNLNLPELKQAYAERITSRLLGNFLLFKTYGDDIRIKKNLQKRKNTPYLT"
BASE COUNT         3508 a         1606 c         1276 g         3772 t
ORIGIN      
        1 ggattcacaa tagctattaa tattttatcc taaacacaaa cattttaacc atcctaagat
       61 tttataaata ttattacctt tttatagatt tataacaaca gtcacctccc ccaaaataat
      121 taatacttaa attatatcat attaaaatct taaaaaagca cataaaaaat aggtagactt
      181 agtctaccta ttcaaaaaat taattatttt ccacaacact ttttatattt ctttccactt
      241 ccacatggac atggttcatt tcttccaact ttaacttctt catttcttac agtttttgaa
      301 tctctgtatc tgaattggat ttctcttctc ttttctcctg agaatatagc atcccattga
      361 ggtaagttaa ataagtaatc tgctttcgca tctaacatat tgtagtataa attttcaaag
      421 tctatatcaa atgataattc tgtattgctt tcaacttttt ctaagtcata tggagttttt
      481 aaactatcgt ttattccatc tacgaatccc attacaaact cttctgttgt accgtattct
      541 ttagctaaat ctgcaattgt agctttctta acttctttgt gattagctaa taaatctttg
      601 tatattttag tttctacatc actatattct ttccagaatg cagcttctcc cttaacctta
      661 acatattcta caaccatgtc tgtccattgc ttatataaac tcatacttcc ttctcctttt
      721 taatcaactt tatatcaaaa ttataataat ttagacaata tatttcaata ttttactaat
      781 taataaacat ataaaatata tattttaata catatgaaat tatttgttta catttttaca
      841 aattccctaa ttatttattt ttaaattagg tatatcttct tttccgaaag gtttactaat
      901 gtcaaaatta ccacctaatg aatcaaccat ttgattatca acaagtatat taatcttatt
      961 aatatttggt atttgggtta ttgtagctat tataccttct aaggttgcct gttctttagt
     1021 tgcactcata tttataatag cttctttgct taaatttatg attgcaatac catcttttat
     1081 atcaaaagat aataatttag tatccttagg taatatagga gctaaacttc ccttttgaga
     1141 tggtccttga ataagagctt gtattaaata ctgccctaaa atttcatcac catcaacaag
     1201 tatttcttgt tgctctattt taggattttg ttcatcctta gttccatcaa agtataaaga
     1261 taaatcaata tctcctatta aatcattagc tattgttttt accctctcgt tattgttagc
     1321 tgctaactta tccattttta tgctgtcaca tccaactaac aaaaaagatg atataattaa
     1381 taaaatcatc ccttttttca tataaattcc ccttacttaa aaacttatat aactacttgg
     1441 tttatgtcta tctgctaact taagctctaa atctttccct atgttaattt tatttgcact
     1501 taatacatta ataacagctt gttcagctaa ttctggcaca ttattagtat tacttaaatg
     1561 acctaatatt atttttctat gcttatcatg attcattata tcaacgatag cttttccgca
     1621 atcttcatta gataaatgtc caacttcact aagtaccctt ctttttagtt cataagggta
     1681 tggtccaaac ttaaccatct gtacatcatg atttgattct aataaaataa cttcagattc
     1741 tctaatattt cttaatattt cctctgtaaa tgttccaata tcagtcgcaa tacttgcact
     1801 ttttcctttg tgctttatag tatagcctgt tgaagccaca gaatcgtgtg gagtattaaa
     1861 agatgttata tctaaatcat ttaaactcat ggtgcttctc ttaggcataa tttttatatt
     1921 ttcttctttt atttttccta gggatttctc catggctttc caagtaagct catttgcata
     1981 tataggtata ttaaattttc ttgataatat acctgctcct tttatatggt cactatgctc
     2041 atgggttatg aaaataccat caatctcatt tggattttga cctacttctt gtaaaccatc
     2101 tattattttt ttgcctgata atcctgcatc aattagtatt ttagtttttt ctgaacctac
     2161 taatatgcta ttaccactgc ttccactata tagtgaacaa aaaatcatat atttccccca
     2221 tttattgttc ttattttctg actatatcag caccaagcat tctgaacttt tcttctatat
     2281 gagggtatcc tctatctatg tgttcaatac ttgttatttc tgtaactcca tcagaaatta
     2341 aacctgcgat aaccattgca gctcctgctc ttaagtcagt agccttaaca acagcccctg
     2401 taagtctatc tacaccatct ataatagctg atcttccttc aacttttata gtagctccca
     2461 tttttttaag ttcatctaca tgtttatgtc tattttccca tatagtttca actattatac
     2521 ttctaccttt agctgtagat agtagagtac acattggttg ttgaacgtct gttgggaatc
     2581 ctggatatgg agcagttttt aaatttactc cttttaaatc tccatcaaca taaactctaa
     2641 cactatcgtc tccctcttct actttagctc ccatttctaa aagcttagct gaaattgatt
     2701 ctaagtgttt tggtataaca ttttgaattg ttacatctcc tcttgtagct gccgccgcta
     2761 tcataaatgt tcccgcttct atttgatcag gtacaacact ataattacaa ccttttaatt
     2821 ccttaacacc tttgattctt atgacatccg tccctgcacc tttaacatta gctcccatag
     2881 catttaaaaa gtttgctacg tctactacgt gtggctctct tgcagcattt tctaacactg
     2941 tagttccttc tgctaaagtt gccgctatca taaggtttat agtagctcca acacttacta
     3001 cgtcaaagaa tatatttgtt cctattaatt tttctgccat tatatttaca gcaccatgct
     3061 ctattgaaac cttagctcct agtgcttcaa atccttttat atgttggtca ataggtctaa
     3121 ctcctattgg acaacctcct ggaagttcaa ctttagcctc tttaaaacgt gctaaaagtg
     3181 ctcctatgaa ataataagaa gctctcattt ttcttacatc atttccacaa gcttctacag
     3241 tttttatttc ttcagcattt atctttaatg tgttattatc taatttttca acattacatc
     3301 ctaatgatct aattattctc tctaagcaat gtacatcagc tatatctggt acattatcaa
     3361 taatacattc acctttgcta gctagtatag ctgccggtaa tatagctacg gcagcatttt
     3421 tagctccgtt tatttctata gatcccttta aagctttacc gccatttata attaatctat
     3481 tcatacaatt cacccttgct aattttttat atgtatacaa ttaccatttt cttctttttt
     3541 attcttgttt aattaaaatt tttatttcga ttaaccatat ttaaactggc taaaccgtga
     3601 aatttaaagt attactcata attttcatta tatcataata agccaatatt taaagatttt
     3661 ttttattgtt tacttttaaa atttacccaa tttatattgt ctaaaaatac tttttattct
     3721 gaaatttctc tcttattatt ttttttaaaa attctatatg ttataattta taacaaagtt
     3781 tgtatttatt tggaacaaac taatattcaa gagaggtttt gttatgaaat attatttagt
     3841 agcattgttt gatgaggagt cttgtaaatt ccttgaatca attcaaagag gtttattgag
     3901 aaaatacaaa ttaccaagaa atcatataag tttacacatt cctcttgaaa caattgataa
     3961 ccccaatcta gataaactag atgaagtggt ccttaaatta atcaaacctt ataagaagtt
     4021 taagatagag ttaactggta gcaccgagtt taatgaaagt actaataaag ctctaaactt
     4081 acaaatcgaa aacaaaggtt atattaaaaa acttcataga ttattcaatg atatgttaaa
     4141 gttacatggt tttaatgtta gagagcgtgt tgactctcct ctatacatac ctataaatcc
     4201 ccctaacttt aaagaaaatc ataaaaagaa taatactcaa cctactgttt ttagcttaag
     4261 agaatcttct caaaagaaca tattaaaagt ttctaaaata gaactttgga gatttcaaaa
     4321 taacaaaaga gaaatccctg taaaatcata cgatctaaga aattactaaa taatttatat
     4381 tgtgtctatt tatattaata tttatatgac atatattcaa ttaacttagg ttaaaataat
     4441 tgtaaattaa catttcgtaa ctttttagat ttgtattcgt tttcgctcct tttcaattgt
     4501 gaaaagattc tgtaaaatta actagtaagc atttttgctt aagaagatag ggggatatac
     4561 tcatggagtt agataatttt tttattcaac aagataagtt taacaaggaa cttgttatcg
     4621 atagtaattt aaatgactat aaagttgcaa ctagaatgta cttagcgctt caagttaaaa
     4681 taagcgaatt agctgatgaa acaaaatgct ttatttattg gaatgatagc aataaatcta
     4741 gagtttcaaa acaattagtt ttagaaaaat atatcgattg tttaaaacaa atccttacta
     4801 taggcttaaa taaaaattat acttgcttaa agaaattaat agttaaacca aatgattatt
     4861 gtttaagcga tcaattttta actttactta tagatgtaaa tgacttaata atatctccat
     4921 ctattgatca ctataaaaca ttatttgaag attttataag cttaggaata agcttaggtt
     4981 tttcaataaa tcaaatagaa gaaatttttt tcaaaaatga ttgttgtaag atagctctct
     5041 aaaatttaga gagttatttt tttatttgtg gaaatactaa gcttgaggtg atttattatg
     5101 cttggtatta ttttagcaat tatttctgga gcagcaatga gtatccaagg agtttttaac
     5161 acaagagttt cagaaaagat aggtgtttgg gaaacaaata caatagttca aggttctgct
     5221 tttgttttag cactttttat tatgcttatg ctaagaaacg gtaattactc tgaacttcgt
     5281 tctgtaaaca aaatatatct acttggagga gcaattggtg ttgttataac atatactgtt
     5341 atggctgctg tgggaagctt aggaccaacc tttggtatat caataatctt aatatcccaa
     5401 ctcttaacgg caggaataat agatactttt gccctttttg atagtgaaaa aatcagattt
     5461 gctgcccaca attatttagg aataatatta atgatagttg gaattatcat ttttaaatgg
     5521 aaaagttaat acattgtcaa aatcatactc ccattcatat attataaaat aataaataat
     5581 atgggagtat ttattatgtt ttcaataaaa caaaaattgg atagcaatct aaaagtttat
     5641 ataaatcgct cttattatac aaattataga gttcttataa aatgcaaaaa atttatggaa
     5701 gatataacaa agaaaatacc caagcttaga ggtattgtta taagagaaat taagtcatta
     5761 aatttgattt gcgctattct tacaccaaaa gctattaata gattaattga atatcctgag
     5821 atagaattta tttcttttga tgaccatgct atactttgtg ggcttagtat aggtactgct
     5881 aatagaattg ccaccaataa atcttttaat tttactggca agaatgtatc tattggttta
     5941 attgatagtg gagtctatcc tcatcaagat ctaacaaatc ctacaaataa aatagatatg
     6001 tttttagatt tattaaataa ctactcttat ccttatgatg ataatggaca tggaactgct
     6061 ttaagtggga ttatatgtgg aagtggatac tcctcaaagc ttgtttttag aggcattgca
     6121 gaaaacacca aaatatcttg tgtaaaggcc tttgatgcta atggaaaggg ctatgtttca
     6181 gatatacttt tcgccattga aactcttata aatcaagaga ataatcctat aagagtttta
     6241 tgtttacctt ttgaacttac cagccataat attaaaatat cagattactt taacgaactt
     6301 tttaaattag cagttagtaa aaatataatt cctgttgtcc cttctggaag tatagaagga
     6361 gataacacta ttcaaggctt agcattatct ccttggtgta taacagttgg tggtatagat
     6421 tctacaaaga caccaacaac aacttttaaa ttttcttcat ctggaaattc taatgtgaaa
     6481 aaaccagatt tttgtgcagc ctgtgctaat ataatgtgct taaactcaga taaaaaatat
     6541 atttctgaaa gaaatggaat aaaactatat cctcataaat tagatagtag ttacacagtc
     6601 tttcaaggaa cctccttagc ctgtgcctac atatctggag tatgtgcact acttttagag
     6661 gccaagccag aactaaatta caaagattta tgttctttat taaaaatagc ttctaataat
     6721 aaatatgaac taccttctga ttctgttgga gaaggagtca tagatttatc atttttactt
     6781 gaaaatattt aaaactaact tatataatct acaggattaa tgggattacc cttatatata
     6841 agttctaaat gcaggtgggg ccccgtactt cttccagtac taccaacttt ggcaatagtc
     6901 tcaccttttt tcacttcttt tccaatctca gttaatatat aactagcatg agcgtagaga
     6961 gtttctgtat tatcatcgtg ctttatctta accatattgc catatatatc gtcataccca
     7021 acaaaactta ctacaccatc acaagcagct cctattggcg ttccactagg cacagctata
     7081 tcaacccctc tatggcctcc tctactcttt tctccaaaga cagatgttat aactcctcct
     7141 ctagttggat gagctaaaaa tgccatattt gagttaattg gattttttat tcccttatat
     7201 atttttgtac tctcagcttc tttcaaaact gattcagata ttatttcact ttctgattct
     7261 ttgccattta caagtattac tttcttttta acctttttct ctcccaattt tccttcttca
     7321 atagtatttt ctcctaaata cttgtcttct agttttataa cttcaactgc tggctctatt
     7381 gatgcaattt cattaatttc agcttcaata gttacatcta gtaaagattc ctctaaaata
     7441 tcattagcaa gcattatctt tgaagttata tcctctactg aacttaaatc tttagattga
     7501 atcatattct tttttaaatc tatttttgta tcaaaattta cttctttaac taaaactccc
     7561 ttttcagaag ccttatctat gtatgtttgt tttattttat ttaaaacagt ttcaactgta
     7621 tcaggatttc ctatataccc tataaccttg ttatttagag ataattcata agcctccaca
     7681 taaacattag caatacaagg catatttaca aaaatatatt ttccacttcc cattcttggg
     7741 tataaagaat tagcaaaaac attagtttta actgtacaaa ataaaataat taaggtaatt
     7801 agaaaaactt taatagagtt tctcccctca ccctctgaaa tactcatatt ggtaattctc
     7861 cttttaataa aaattttagt ataaatttaa acacgtaaat taatgttccc acttaacaaa
     7921 aaattattca tgtaaataaa atattttttt gcaatttata gaagattact ttattgttat
     7981 agaaaagaca ctttgatata attagacata gtctattttt tatttatata cattttaaaa
     8041 tttagactat ttataaatct agaaaggtgg ttattttttg agtactttta tgttaaaaaa
     8101 ctcagccact aaatttactc ctgttagtaa tgtattccta gaagaatata tgcctaaagc
     8161 tcgtggagaa tttattaagg tttatttatt attgctaaag tataatttca ctggagaacc
     8221 tggagttaac tcttcaattt tagcaacaaa cttaaatctt ttagaatcag atataatgaa
     8281 tgctcttaat tattggcatg acgaaggtgt tataaaacta gttcctatag ataaaatgaa
     8341 taactttaaa atagaatttt tagacttatc agaaggtaat cattcatcaa gtcaaaactc
     8401 tgttgattta ttatcagccc ttgatgaaaa taacactaag gatatgctca aagatataga
     8461 aaagcttata tgtcgtcctt tatccacaaa ggaaatgaca acttatcttt cttggcaaac
     8521 agagcttaat ttttcctcag agcttatatt aatactaata gagtactgtg tatctaaggg
     8581 taaaaaagat gttagataca tagaaacagt ggctcttgga tggcataatg ccggaataaa
     8641 atcaatagag gaagctcaag cttatataac taaaacagaa gacaaatggg ttaaaataag
     8701 aaaaattctt aagtatttag gaattcaaaa taccgaaata atgaagccac aggaaactat
     8761 gatggaaaag tggcttttaa cttataactt ctctgttgag ttaatactta aggcttgcga
     8821 tatatgcttt gaacgtttaa acagagctga ttttaaatat atagatggta tcttaggtaa
     8881 atggtttaaa gatggtataa agactcttga tgatgtagca aaaaaagata ttaaaaagac
     8941 atctagtatt aaaaaaccat ttaacaaaaa taattcagct ccaattcaaa agaaaccagc
     9001 tagagaagca aactttaccc aaagagatta tgactatgat tccttagaaa aacaattgtt
     9061 aggatgggat attgaatgat taaaggttat aaaagtcaat tgatggatat gtatgaaaaa
     9121 atccgtcaaa aagaaaaatc aaatctagct aatagacaaa aagagttaaa agaaaagcta
     9181 cctcaagttt ttgagtatga aagagaaata aataagcttt ctttaaaact ttcaatgctt
     9241 gctttaagaa aacatgaaaa agaaagtttt gaaaaattaa aaaaagaaat tgaaaatctt
     9301 agagatgaaa aagatcaact acttgctatg agtggatatc caaaagatta tttagatatg
     9361 cattatatct gtccatcttg caaagataca ggatatatag gcactaaaaa atgtatttgt
     9421 tataagaaaa aacttgtaga catatactat gaaaattctc atatggctga tgctttaaag
     9481 ctaaataatt tttcaaaatt tgatataaat gttttttctc ctttaaacaa tggtaatgaa
     9541 aaatattctc caagagaaaa tatgcaaata atcattgata aaatggttaa tggatatatc
     9601 cctaaatttg atacgcataa tgaaaatata cttttctatg gtagtcctgg aactggaaaa
     9661 acatttttaa cttattgcat agctaaagaa ttattagaca atggatttct tgtagtttat
     9721 agaacatcag atgaacttat tagaaatctt agagaaataa gatttaataa tgatcatgaa
     9781 ttagaagatt tgcttataaa ttgtgactta cttgttatag atgacttagg tgcagaacaa
     9841 ataactgatt tctctacaac tgagttattt acccttctaa acaagaaaat attattaaat
     9901 aaaaagatga ttatatctac aaatttaaat cttcctgaat taaagcaagc ttatgcagaa
     9961 agaataactt ctagattgtt aggaaacttt cttctattta aaacttatgg agatgacata
    10021 agaataaaga aaaatcttca aaaaagaaaa aatacacctt atcttactta aaagataagg
    10081 tgtacttttt ttgcataaat aaattaaatg gcgaattact ctctaaaaga ataaaaaaaa
    10141 acttcagttt aactgaagtt ct
//
LOCUS       DAEVGI010000089        10138 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEVGI010000089 DAEVGI010000000
VERSION     DAEVGI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10138
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.089"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            174..980
                     /gene="spoIIGA"
                     /locus_tag="K8M77_001742"
     CDS             174..980
                     /gene="spoIIGA"
                     /locus_tag="K8M77_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBI7086234.1"
                     /translation="MTVYIDIVILENFLINFFLLYLTLQILKDTIIYKRVILSAFLGA
                     IYTLFVFIPGLNILTSLPLKLLFSFGMITIISERRELKTIIKRYITFLVITFAFSGTC
                     FMFALVENQYNISESFIINDYSTKSIIFSLIISYILVSGVMNYFKNRAIINNFIYDLD
                     VCIDSEVVNIKAFLDTGNGLVEPATALPVIIAEREKFRGINIKEKDQFRIPYKVVDGN
                     SGYMKGIKIDNIKLCNVNGETMTRDAILCFCDNKLSKEGEYEALLSRGII"
     gene            994..1701
                     /gene="sigE"
                     /locus_tag="K8M77_001743"
     CDS             994..1701
                     /gene="sigE"
                     /locus_tag="K8M77_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBI7086235.1"
                     /translation="MLNIRLLLNKLFTKLDIMVEPIYYVGGNDVLPAPLSKEEEEELV
                     NKLGENDEAIRSILIERNLRLVVYIARKFENTGVGVEDLISVGTIGLIKAVNTFNPEK
                     KIKLATYASRCIENEILMYLRRNSKVKAEISFYEPLNIDWDGNELLLSDILGTDDDIV
                     YNLIEDEVDKELLFTAMKNLSNREKEIVELRFGLCGYKEKTQKEVADMLGISQSYISR
                     LEKKIIKRLKKEINKMI"
     gene            1776..2549
                     /gene="sigG"
                     /locus_tag="K8M77_001744"
     CDS             1776..2549
                     /gene="sigG"
                     /locus_tag="K8M77_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBI7086236.1"
                     /translation="MMINKVEICGVNTAKLPVLKEKEMKELLLQMQNGDTSAREKFIK
                     GNLRLVLSVIQRFNNRGENVDDLFQVGCIGLMKSIDNFDLSQNVKFSTYAVPMIIGEI
                     RRYLRDNNTIRVSRSLRDIAYKALLVRDKLVNENNKEPTVSQIAKELKIPREDVVFAL
                     DAIQDPVSLFEPIYYDGGDAIYVMDQISDSKNQDDSWLENIAIKEAMKKLNDREKLIL
                     NLRFFNGRTQMEVADEIGISQAQVSRLEKIALKHMRKYV"
     gene            2690..2962
                     /locus_tag="K8M77_001745"
     CDS             2690..2962
                     /locus_tag="K8M77_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBI7086237.1"
                     /translation="MEENLFSLNNLRAMEVIDVSEGKKLGLIADIKIDCDDNRILSII
                     IPGEKTSFFSKSEDIEIEWSDVFKVGVDVILIDSKGKNILDVQKYI"
     gene            3024..3473
                     /gene="nrdR"
                     /locus_tag="K8M77_001746"
     CDS             3024..3473
                     /gene="nrdR"
                     /locus_tag="K8M77_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBI7086238.1"
                     /translation="MRCPYCSYEESKVVDSRSAEDYNAIRRRRECLRCSKRYTTYEKV
                     EDIPILVIKKDLSRESFNKEKIISGLIKACQKRPVSRAQIEEIASDIERNISNKMMVE
                     IKSDYIGEMIMERLKDIDEVSYVRFASVYRQFKDINTFMEEIKNLMK"
     gene            3639..4343
                     /locus_tag="K8M77_001747"
     CDS             3639..4343
                     /locus_tag="K8M77_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HBI7086239.1"
                     /translation="MKREIIDNKEFIVFNDGNIKVRFSTALNNVNYKKEDEEGKKNLE
                     DLKEIFKVDKVLYVNQTHSSDFIDATFENFKGDRDCDSLVTKDKNTLIGVFTADCVPV
                     IAYDKEKEVIAAIHSGWKGTYTKIARKTCEYMKEKYGCENIKVIIGPHVRQCCYEVSE
                     DLAEKFSEEFGKEVCNGRMLNLEKCVEIQLKGIVKKENITSTRICTYCEKEVKMHSYR
                     QDQEKSGRLFSSIFID"
     gene            4357..6072
                     /locus_tag="K8M77_001748"
     CDS             4357..6072
                     /locus_tag="K8M77_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBI7086240.1"
                     /translation="MKKRIIIFTTLIITFFLAIMTSMYLVISNNKYLEESKNILNEYN
                     KVIALLLENDNGNIKSELERIESNNDMKNIRITYISKDGNLIFDTHKKLISDNESYLK
                     RQEIIEAIESGLGSSVRYSNDLHQNMIYSALKLKDGSIVRTSIAVENAKILDSINSNY
                     LLVGVIFSLVIALLLTVKITNIILNPLKELEQLTSTIASGNFHKRVKINSKDDEIQRL
                     GKSFNYMAEQLEITMERFKNKQNGLEAILKSMGSGVIAFDRDMNVLMINPYAKKIFGI
                     SGEIIGNKLLDYITDKEVLKAFFDEKDRVEIEVNYNDDPKILKIRKASIINEPEIIGT
                     VVVIQDITDIKKLENMRSQFVADISHELKTPLTSIKGFAETLRYVDDDETRNKFLSII
                     DEESDRLARLLEDILCLYEIEQKRSTVLEEFNVDEEIEKVYMLLNDQAKKKGVEIFLD
                     THSNCVLMGDKDKFKQMLLNLVSNSVKYTEKGGKVRVESYNRDMNLILVIEDNGIGIS
                     AEDLPRIFERFYRVDKARSRESGGTGLGLAIVKHIVRLFDGEINVTSELGVGTKIVIT
                     IPINI"
     gene            6399..7736
                     /locus_tag="K8M77_001749"
     CDS             6399..7736
                     /locus_tag="K8M77_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI7086241.1"
                     /translation="MKSRIAEVLPGGIGEELGFEKGDILLSINGTKVEDILDYRFLLA
                     DEYVEVEILKPNKEVWEFEIEKEYGEDLGVEFEEAILDKAKSCTNKCIFCFIDQLPKG
                     MRKTLYFKDDDSRLSFLQGNFVTLTNMKDEDIDRIIKYRISPINVSVHTTNPDLRKKI
                     LNNRFAGNVYERLQKLAAAGITVNAQIVVMPGINNGDELVRTVEDLYKLRPSIENVAA
                     VPIGITKFREGLADLEIYNKETAKEELDRIKVLQEKYMKEIGSPFVRLSDEFYVMADE
                     EVPNEEFYNGYEQIEDGVGMIRLLRETMAVDIKNKLTKSGKGKFTLITGTSAFKELDK
                     VCTDIREENNNIDIKCKVILNDFFGHTITVAGLLTGQDVIAQLKDNIDSEYLIMADNM
                     FRKGYEPGDITQRIMLDDLTAKDIEERLGVKVIVCSYTGEDLIDLINEHCEEE"
     gene            7739..9055
                     /gene="der"
                     /locus_tag="K8M77_001750"
     CDS             7739..9055
                     /gene="der"
                     /locus_tag="K8M77_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012449707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBI7086242.1"
                     /translation="MSKPIVAMVGRPNVGKSTLFNKLAGKRISIVQDTPGVTRDRVYA
                     ESEWLNRKFTMIDTGGIEPESSDIIVKQMRRQAQIAIEMADVIVFVVDGKEGLTAADQ
                     EVAQMLRKSKKPVVLVVNKIDRLVLEENSYEFYNLGIGDPITISASQGLGLGDMLDEV
                     VKYFNDPSEDEEDDEYIRIAMIGKPNVGKSSLINRLLGEERVIVSNVPGTTRDSIDSY
                     LETEDGKFILVDTAGLRRKSKVKEEIERYSVIRTYAAIEKADVAILVIDAEQGITEQD
                     EKIIGYAHEMNKAIMVVVNKWDLIEKDDKTLSNYQKDLQQKLKFMPYAKYLFISALTG
                     QRVHKILSTAKYCYDNYSKRVSTGLLNDVISKAVLMKEPPVVALKRLKIYYATQVATK
                     PPKFVFFVNDPNLLHFSYGRYLENQLRESFDFDGTGIEIEYRARKE"
     gene            9060..10055
                     /locus_tag="K8M77_001751"
     CDS             9060..10055
                     /locus_tag="K8M77_001751"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBI7086243.1"
                     /translation="MSKVAFLGAGSFGTSLGILLGNKGVTVSLWDRDENVINDINVNR
                     KNDKYIKDLTIPTNVTAYKDLDEALNGAEYVVLAVPSHVIRTACKNLKGKINDDVIII
                     NIAKGIEEGTNLRLSQVINQELPNNKVVVLSGPSHAEEVSKGIPTTLVASSECMECAE
                     KVQDLFMDKNFRIYTNDDIIGVEIGGAVKNIIALAAGVCDGIGYGDNSKAALMTRGMA
                     EIARIGIKMGGKAETFFGLTGMGDLIVTCTSMHSRNRRAGILIGQGKTAEEAIKEVGM
                     VVEGIKACKAFYELKEKEGVTMPITDIAYKVLFEGAKAENAVSLLMERDKKKEEI"
BASE COUNT         4151 a          917 c         1931 g         3139 t
ORIGIN      
        1 cattacaaga atagacatga aaaaaataat tataatatta agaatttttt acttggtaga
       61 aaaatgtaga aaaatgagag aaaagaaaga gagaaaatat gcatatggaa tatataatga
      121 caaaatttta gaataagaag ctttataatt ataaataggg agtgagttga tttatgactg
      181 tatatattga cattgtcata ttagaaaatt ttttaattaa cttttttcta ctttatttaa
      241 ctttacagat cttaaaagat acaataattt acaagagagt aatattatca gcttttttag
      301 gagcaatata taccttgttt gtttttattc caggattaaa tatactcaca tcattgcctt
      361 taaaactact tttttctttt ggaatgatta ctataatatc agaaagaaga gagttaaaga
      421 caataataaa aagatatata acttttttag tgataacttt tgcctttagt ggtacttgct
      481 ttatgtttgc tttagttgaa aatcagtata atatttcaga atcctttata ataaatgatt
      541 attcaactaa atctataata ttctcactaa ttataagtta tatacttgtt agtggagtta
      601 tgaattattt taaaaataga gctattataa ataactttat atatgattta gatgtatgta
      661 ttgattcaga agttgttaat attaaagctt ttttagatac aggaaatgga cttgtagagc
      721 ctgcaactgc attgcctgta ataatagcag aaagagaaaa gtttagaggg ataaacatta
      781 aggaaaaaga tcaatttagg ataccgtata aagtagttga tggaaattca ggatatatga
      841 aagggattaa gattgacaat ataaagcttt gtaatgttaa tggggaaaca atgacaagag
      901 atgcaatctt atgtttttgt gataataaat taagtaaaga gggagaatat gaagctttat
      961 tgtctagagg aattatatag gggtgattag gttatgttaa atataagact tttgcttaat
     1021 aagttattta ctaaattaga tattatggta gaaccaatct attatgttgg gggaaatgat
     1081 gttcttccag cacctcttag taaagaagag gaagaggagt tagttaacaa attaggggag
     1141 aatgatgaag ctattagaag tattcttatt gagagaaatt taagactagt tgtatatata
     1201 gctagaaaat ttgagaacac tggtgtagga gttgaggatt taatttctgt aggaacaatt
     1261 ggacttataa aggcagtaaa tacatttaat ccagaaaaaa agataaagct tgcaacctat
     1321 gcatcaagat gtatagaaaa tgaaattctt atgtatttaa gaagaaatag caaggttaaa
     1381 gctgagatat cattttatga gcccttaaat atagattggg atggaaatga acttttactt
     1441 tcagatatac ttggtacaga tgatgatatt gtttacaatc taatagaaga tgaagttgat
     1501 aaggaattgt tatttactgc aatgaagaat ctttcaaaca gagaaaaaga aattgttgaa
     1561 ttaagatttg gactatgtgg atataaagaa aaaactcaaa aggaagtagc tgatatgcta
     1621 ggaatatctc aatcttatat ttcaaggtta gagaagaaaa ttataaaaag attaaagaaa
     1681 gaaattaaca aaatgattta agagtataaa attaacctct acaggtaata attaaaatgc
     1741 ggaagggaat tacttcaaag gggttgattt caattatgat gataaataaa gtagagatat
     1801 gtggagtcaa tacagctaaa ttaccagttt taaaagaaaa agaaatgaaa gaattattgc
     1861 tacaaatgca aaatggagat acaagtgcta gagagaaatt tattaagggg aatctgagat
     1921 tagtgcttag tgtaatccaa aggtttaata atagaggcga aaatgtagat gatttatttc
     1981 aagttggttg tataggactt atgaaatcta tagataactt tgatttatca caaaatgtta
     2041 aattttcaac ttatgctgtg ccaatgataa taggagaaat aagaagatat ttaagggata
     2101 ataacactat aagagtaagt agatctttaa gagatatagc ttataaggct ttacttgtaa
     2161 gagataagct agtaaatgaa aataataagg aacctactgt atctcaaata gcaaaagagc
     2221 ttaaaattcc aagggaggat gttgtttttg ccctagatgc aattcaagat ccagtttctt
     2281 tatttgagcc tatttattat gatggtggag atgcaatata tgtaatggat cagataagtg
     2341 attcaaaaaa ccaagatgat agttggcttg aaaatatagc aataaaggaa gctatgaaga
     2401 aattaaacga tagagaaaaa cttatattga atttaagatt ctttaatgga agaactcaaa
     2461 tggaggtagc tgatgaaatt ggtatatctc aagcgcaagt ttcaaggttg gaaaaaatag
     2521 cattaaaaca tatgaggaag tatgtataga gctcttttga gctctatttt ttatggataa
     2581 attaaaaatc ttatgttatt tagctttttt tacctcttct aattaataaa tataaaaatt
     2641 aaacatataa attaaatata ataattatat gtttaggagg agtttataca tggaagaaaa
     2701 tttattttct ttaaataatt taagggctat ggaagtgata gatgtaagtg aaggtaaaaa
     2761 gttaggctta atagctgata ttaagataga ttgtgatgat aatagaatat tatctattat
     2821 aatacctgga gaaaaaacat ccttttttag taagtcagag gatatagaga tagaatggtc
     2881 tgatgtattc aaagttggag tagatgttat actaatagat tcaaaaggaa aaaatatact
     2941 agatgttcaa aaatatatat agaaattaag taaaaaagag ggtataatat tattacaact
     3001 tagagaaagg cgtggatttt aaaatgagat gtccttactg ttcttatgaa gaaagtaaag
     3061 ttgtggattc tagatctgca gaagattata atgcaattag aagaagaaga gaatgtttaa
     3121 gatgttctaa aagatacaca acttacgaaa aggtagaaga tataccaata cttgttataa
     3181 aaaaggattt aagtagagaa agtttcaata aagaaaaaat cataagcgga ttaattaaag
     3241 cgtgtcaaaa gagacctgta tctagggctc agatagaaga aatagcatct gatattgaac
     3301 gaaacataag taataaaatg atggtagaaa taaagtctga ttacataggt gaaatgataa
     3361 tggagagact taaggatata gacgaagttt catacgtaag atttgcttct gtatatagac
     3421 aatttaaaga tattaatacc tttatggaag aaataaaaaa tttaatgaaa taattttaag
     3481 ctatcttaaa gaaagttctt taagatagct tttttatttg ttttaattta tccatatact
     3541 taaataatat ttaacttgaa tttaatttaa taaagagttg aaatatgata atatggtaaa
     3601 ataagtagat tataatttgt gtttttgagg tgaaagttat gaaaagagaa ataatagata
     3661 acaaagaatt tatagttttc aacgatggaa atataaaagt tagattttca actgcattaa
     3721 ataatgttaa ttataaaaaa gaagatgaag agggaaagaa aaatttagag gacttaaagg
     3781 aaatatttaa agtagacaag gtactttatg ttaaccaaac acatagtagt gattttattg
     3841 atgcaacttt tgaaaacttt aaaggggata gagactgtga ttccttagtt actaaggata
     3901 aaaacacact tataggagtt tttacagcag attgtgttcc agttatagct tatgataaag
     3961 agaaggaagt aattgcagct attcatagtg gatggaaagg aacatacact aaaatagcta
     4021 gaaaaacttg tgaatacatg aaagagaaat atggttgtga aaatattaaa gtaataatag
     4081 gtcctcacgt aagacaatgt tgttatgaag taagtgaaga tttagcagag aagttcagtg
     4141 aagaatttgg gaaagaggta tgcaatggaa gaatgctaaa tcttgagaaa tgtgtagaaa
     4201 ttcagcttaa gggtattgtt aaaaaagaaa atataacttc tactagaatt tgtacttact
     4261 gtgaaaaaga agtaaagatg catagttata gacaggatca agaaaaatca ggaagattat
     4321 tttcatcaat atttattgat taaggtggat taataaatga aaaaaaggat tattattttc
     4381 acaacactaa taataacatt ttttctagct ataatgactt ctatgtactt agtaatatca
     4441 aataataaat atttagagga atcaaagaat atcctaaatg aatataataa ggtcatagct
     4501 ttattgttag aaaatgataa tggaaatata aagagtgaat tagaaagaat agaatcaaat
     4561 aatgatatga aaaatataag aataacatat atcagtaagg atggaaatct tatttttgat
     4621 actcataaaa aattaataag tgataatgaa agttatctta agagacaaga aataatagaa
     4681 gccatagaaa gtggtttagg aagcagtgta agatatagta atgatctcca tcaaaacatg
     4741 atctatagtg cactaaaact taaggatggc tctattgtta gaacatcaat agctgttgaa
     4801 aatgcaaaaa tattagatag cataaatagt aactatttat tagtaggtgt tatattttcc
     4861 ttagtaattg ccttactttt aactgttaaa ataactaata taatattaaa tccactaaag
     4921 gagttagaac aattaacctc tactattgca agtggtaatt ttcataaaag agtaaaaatt
     4981 aattctaagg atgatgaaat tcaaagacta ggaaaaagct ttaattatat ggcagagcaa
     5041 ttagaaataa ccatggagag gtttaagaat aaacaaaatg gattagaagc catattaaaa
     5101 agtatgggta gtggagtaat agcttttgac agggatatga atgttttaat gataaatcct
     5161 tatgctaaaa aaatatttgg cataagcgga gagattattg gaaataaact tttggattat
     5221 ataactgata aagaggtatt aaaggccttt tttgatgaaa aagatagggt tgaaattgaa
     5281 gttaactata atgatgatcc gaaaatatta aaaataagaa aagcaagtat aataaatgaa
     5341 ccagaaataa tagggacagt tgtggttata caagatatta cagatattaa aaaacttgaa
     5401 aatatgagaa gtcaatttgt agctgatata tctcatgaac ttaagacacc acttacctca
     5461 attaaaggtt ttgcagaaac cttaagatat gtagatgatg atgaaactag aaataaattt
     5521 ttaagcataa tagatgaaga atcagataga ttagcaagac ttttagagga tatattatgt
     5581 ctttatgaaa tagaacaaaa aagaagtact gttttagaag aatttaatgt tgatgaagaa
     5641 attgaaaaag tttatatgct attaaatgat caagctaaga aaaagggtgt ggaaatattt
     5701 ttagatacgc atagcaattg tgttcttatg ggagataagg ataagtttaa acaaatgtta
     5761 cttaatcttg taagcaattc tgttaaatat actgaaaaag gtggaaaggt aagagttgaa
     5821 agttataatc gtgacatgaa tcttatttta gttattgaag ataatggtat tggaataagt
     5881 gcagaggatc ttccaaggat atttgaaaga ttttatagag tagataaagc taggagtaga
     5941 gaaagtggtg gaactggact aggtcttgcc atagttaaac atatagttag actttttgat
     6001 ggtgagataa atgtaactag tgaactagga gtagggacta aaatagtaat aactatacct
     6061 ataaacatat aattctagtt aattaatact ttataacacc tttaaattta taacaattat
     6121 tatataatta gttatatatt taaaaatttt tttactataa tagaattaag agaaagctat
     6181 taaaagtaat cttcaaaatt tttttagggg attacttttt aatatttaaa caacatagtt
     6241 taaagcttaa tgaaaaagtt aataattaga tttagagtta aataaaaagt tagtaattga
     6301 aacaaaacac aatatatagg tataaagtta tttcagttga ctacattatt taattaaggt
     6361 aatataacat agatagtact aaaataggag tgaagcatat gaaaagtaga attgctgaag
     6421 tcttaccagg aggaattggt gaggaattag gctttgaaaa gggagatata ctattaagca
     6481 taaacggtac taaagttgaa gatatacttg attatagatt tttattagca gacgaatacg
     6541 ttgaggtaga aatattaaaa ccaaacaaag aggtttggga gtttgaaata gaaaaggaat
     6601 atggcgaaga tttaggagtg gaatttgaag aggccatcct agacaaagct aaaagttgta
     6661 caaataaatg tatattttgc ttcatagatc aattaccaaa gggaatgaga aaaactttat
     6721 attttaagga cgatgattca agactttcat ttttacaagg taactttgtt actttaacaa
     6781 atatgaagga tgaagatatt gatagaatta taaaatatag aataagtcct ataaatgtat
     6841 cagttcatac aacaaatcca gatttaagaa aaaaaattct taataataga tttgcaggga
     6901 atgtatatga aagactacaa aaattagcgg cagctggtat aactgtaaat gctcaaatag
     6961 ttgttatgcc aggaataaac aatggagatg aattagttag gactgtagaa gatctttata
     7021 aattacgtcc ttcaatagaa aatgttgctg ctgtaccaat tggaataaca aagtttagag
     7081 aaggattagc tgaccttgaa atatataata aagaaacagc taaagaagaa ttagatagaa
     7141 taaaagttct tcaagaaaaa tatatgaaag aaataggaag cccatttgta agattatcag
     7201 atgagtttta tgttatggca gatgaggaag ttccaaatga agaattctat aatggatatg
     7261 agcaaataga agacggagtt ggaatgataa gacttctaag agaaaccatg gcagtagata
     7321 taaagaataa gttaactaaa agtggaaaag gtaaatttac tcttattaca ggaacatctg
     7381 cctttaaaga attagataag gtttgtaccg atataagaga agagaataat aatatagata
     7441 ttaagtgtaa ggttatatta aatgatttct ttgggcacac aataacagtt gcaggacttt
     7501 taacagggca agatgttatt gctcaactta aagataacat tgatagtgag tacttaatta
     7561 tggcagataa tatgtttaga aaaggttatg agccaggaga tataactcaa agaataatgt
     7621 tagatgattt gacagcaaag gatattgagg aaagattggg agttaaagtt atagtttgta
     7681 gctatacagg ggaagaccta atagacttaa taaatgaaca ctgtgaggag gaataagaat
     7741 gagtaaacca atagttgcta tggttggaag accgaacgta ggtaagtcga ctcttttcaa
     7801 taaattagca ggaaaaagaa tttcaatagt acaagataca ccaggggtta ctagagacag
     7861 agtatatgca gaatcagaat ggttaaacag aaaattcaca atgatagata caggtggaat
     7921 agagcctgaa agtagtgata taattgttaa acaaatgaga agacaagcgc aaattgctat
     7981 agaaatggct gatgtaatag tattcgttgt tgatggtaag gaaggactta ctgctgctga
     8041 ccaagaagtt gcacaaatgc ttagaaaaag taaaaagcct gttgttttag tagttaataa
     8101 aatagataga ttagttttag aagaaaatag ctatgagttc tataatttag gaattggaga
     8161 tcctataact atatcagcat ctcaaggatt aggacttgga gatatgctag atgaggttgt
     8221 taaatatttt aatgatcctt cagaagatga agaggatgat gaatatatta gaatagctat
     8281 gataggtaaa ccaaatgtag gtaaatcatc acttataaat agattattag gtgaagagag
     8341 agttatagta agtaatgttc caggaacaac aagagattct atagatagtt acttagaaac
     8401 agaagatgga aagttcatct tagttgatac tgctggatta agaagaaaaa gtaaagtaaa
     8461 agaagaaata gaaagatata gtgtaatcag aacttatgct gccatagaga aagctgatgt
     8521 agctatactt gtaatagatg ctgagcaagg aataactgag caagatgaaa aaataatagg
     8581 atatgctcat gaaatgaata aagcaattat ggttgttgta aataaatggg atcttattga
     8641 aaaagatgat aaaacattaa gtaattatca aaaagactta caacaaaaac ttaagtttat
     8701 gccatatgct aaatacttat tcatatcagc tttaacagga caaagagtac ataaaatatt
     8761 atcaacagct aaatattgtt atgataatta ctctaagaga gtttcaactg gattattaaa
     8821 tgatgttata agtaaggctg ttttaatgaa agagccacca gttgtagcct taaagagatt
     8881 aaaaatatac tatgctactc aggttgctac aaagccacct aagtttgtgt tctttgtaaa
     8941 tgaccctaat ttattacatt tctcatatgg tagatattta gaaaaccaat taagagaaag
     9001 ttttgatttt gatggaactg gtatagaaat agaatataga gctagaaagg agtaatttta
     9061 tgagtaaagt ggctttttta ggagcaggaa gttttggaac ttctttaggt atattattgg
     9121 ggaataaagg tgttacggtt tctctgtggg atagagatga aaatgtgata aatgacatta
     9181 acgtaaacag aaagaatgat aaatatataa aagatttaac tattcctact aatgtcactg
     9241 cttataaaga cttagatgaa gctttaaatg gggctgaata tgtagttctt gcagtacctt
     9301 ctcatgttat aagaacagct tgtaaaaact taaaaggtaa aattaatgat gatgtaataa
     9361 tcattaatat tgctaaagga atagaagagg gtactaattt gaggttatct caagttataa
     9421 atcaagaatt accaaataat aaggtcgtag ttttatctgg gccaagtcat gcagaagagg
     9481 tttcaaaagg aattccaaca actttagttg caagttcaga atgtatggaa tgtgcagaaa
     9541 aagttcaaga cctatttatg gataaaaact ttagaatata tactaatgat gatatcatag
     9601 gtgttgaaat tggaggagcc gttaaaaaca ttatagcctt agcggcaggt gtttgtgacg
     9661 gtataggata tggagacaat tctaaggcag cattaatgac tagaggaatg gctgaaatag
     9721 ctagaatagg aattaaaatg ggtgggaaag ctgaaacttt ctttggatta actggtatgg
     9781 gagatttaat agttacttgt acaagtatgc attcaagaaa tagaagagct ggaatactta
     9841 taggtcaagg aaaaactgct gaagaagcta taaaagaagt aggaatggtt gttgaaggta
     9901 taaaagcttg taaagccttc tatgaattaa aagaaaaaga aggagtaaca atgcctataa
     9961 ctgatatagc atataaagtt ctttttgaag gggcaaaggc agaaaatgct gtaagccttt
    10021 taatggaaag agataagaaa aaagaagaaa tataaaattt gatttaaagg agatagaaaa
    10081 ttttctatct ctttttgttt taattaaaag tggctagtgt taaaaaattt tatgtagt
//
LOCUS       DAEVGI010000090        10073 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEVGI010000090 DAEVGI010000000
VERSION     DAEVGI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.090"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(182..1495)
                     /locus_tag="K8M77_001752"
     CDS             complement(182..1495)
                     /locus_tag="K8M77_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBI7086244.1"
                     /translation="MKQKSKKYLKYISSNNTFLLGKTFLKWLLIGSLIGIFIGGVIAI
                     FLKSLEWATDYRVNHGFVLFLLPIGGALVSFLYYKFGRNSVKGNNLIIENINNYCGDV
                     PLRMVPLVFFGTVVTHFFGGSAGREGTGVQIGASIAETIGKLLKLNKEDSKIMLMAGV
                     SGGFSAIFGTPLAGTIFGLEVSVLGKMSYEALIPSFFASIVGNEVVKNLGVHHSHYKV
                     LGVPDISALIILKVIISAICFGLASRLFSELTHKLKKVFSNKFKNTSLKSFVGGCIVI
                     LLVYLVGSRKYLGLSLPLLSEAFNGHISPFDFLGKLIFTSITLGAGYQGGEVTPLFVI
                     GSTLGNTLSGILNLSPSFLASLGLVGVFAGATNTPIASFVLGIEMFGVGGAQYLFMAC
                     AISYIFSGHTGIYTSQKIGVSKSNLISFSHDSTLASLSKREEEKL"
     gene            complement(1753..2412)
                     /locus_tag="K8M77_001753"
     CDS             complement(1753..2412)
                     /locus_tag="K8M77_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBI7086245.1"
                     /translation="MDTKELLLSESNEMISGIYERIQSLESLDLKSLDKNRTILLIVD
                     INKGFAKAGALYSDRIEKLINPISNLAKYALNNGIKVKAFTDYHTEDSIELKAYPKHC
                     MKDTDEWELVEELNLEGIEVIKKNSTNGFLEENFILNKEEIDNVIIVGDCTDICIYQL
                     AISLRAEFNRVNKDGEIYVPKKLVDTFDMPMHKANFMNYVFLNSMIDNGVNVIEDIIL
                     D"
     gene            2670..3770
                     /locus_tag="K8M77_001754"
     CDS             2670..3770
                     /locus_tag="K8M77_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI7086246.1"
                     /translation="MNNFFRNNKSYIKMVLVIVVSYLSIKLIDNIPSVMSAIHTILAV
                     LLPFLLAFIIAYILNPIVNLFSKKFKLSRGISIVITYLIFVTSISLAATYLLPKLYTS
                     LIELIDNIPAFTNYVQNLFTEIMASFKVPEGINSYLPHGDTNKLITFIGSMVTLLSNW
                     LLDAAISLTSSIINWIFGFLISIYVLVDKDKFLAHSSKVTRIIFGKNLGDEILEFIKI
                     LNEKIGTYVGIKAIDSLIIGLLAFVGLTVIKSEYALLLSVLVGITNMIPYFGPFIGMV
                     VGFFINVFISPMKAVIVLIYLFILQQFDAWYLDPKLIGNRVGLSPFLVILGVTLGGAI
                     YGPIGMILGSPVMSVIRIYTLKLIKKFEYRAE"
     gene            complement(3820..4272)
                     /locus_tag="K8M77_001755"
     CDS             complement(3820..4272)
                     /locus_tag="K8M77_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086247.1"
                     /translation="MLNKSDNEIYIMAPGEGNVLDYIVLNKAFNLEDLEEIISLNSHN
                     EFLFSPVNGKVICISKDYGFIEIGMKEGVNIIVSTHINNNIKAIEPIVDIGGEVLTGQ
                     ILMYFEKESDCDMSITVAIKKSHSILKIVKSKNKNIVLNEYIMKLNLI"
     gene            4563..5309
                     /locus_tag="K8M77_001756"
     CDS             4563..5309
                     /locus_tag="K8M77_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI7086248.1"
                     /translation="MLSEKRHEIILNLLKLKGFVSLAELLEATESSESTIRRDLSYLE
                     SINLLKRVHGGAKSLTNVSKELSYNEKSSKSIHEKRAIAKYAASLIEDGDCIFIDAGT
                     TTYELIDYLEGKDILVVSNGLSHIDTLIQKNIKCYVIGGNIKISTKAITGCDALMCLS
                     KFRFDKCFIGANGVHHIYGLTTPDTEEAILKECAINNSRKSYVLADDSKFGEISTIKF
                     SDINKCVIITNEKPKDNAYDKKTDIKVVDI"
     gene            5309..6229
                     /gene="pfkB"
                     /locus_tag="K8M77_001757"
     CDS             5309..6229
                     /gene="pfkB"
                     /locus_tag="K8M77_001757"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBI7086249.1"
                     /translation="MIYTLTLNPSIDYVIDVENLELGKVNRTSKDFKFPGGKGINVSR
                     ILGNQKIDNIALGFLGGFTGKFIEDNLKALGVKTEFIKVKGDSRINVKIKSNEETEIN
                     GTGPMIASEELEALFNRLDQLVEGDILVLAGSIPSTLPKDLYLQIQKRVSKKNVKVVV
                     DTSGKALLEAIKNKPFLIKPNNHEIEEIFNVKINSEKELIKYGKKLVSLGAENVIISM
                     AGDGALLITKDGVFKGNAPKGEVKNSVGAGDSLVGGFLAKYVETKDLIASFKNGIASG
                     SASAFSLDLALAEDVNKLLPIIKIEKYEEV"
     gene            6233..8125
                     /locus_tag="K8M77_001758"
     CDS             6233..8125
                     /locus_tag="K8M77_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI7086250.1"
                     /translation="MKISQLLSLDTIKLDLAASNKNEVIDQLVDVLWDAGKLTDKNLY
                     KEEILKRESLSSTGIGERIAIPHAKTKAVKNASLALGLYKDGVDYDSLDGEPVNIVFM
                     IAAPDGANDEHLETLSRLSVLLMNPDFKNGLLNAKTPEEVISLLDKFEENEESDEINN
                     SNEIENNSNKKYILAVTACPTGIAHTYMAADSLNKTAKEMDINIKVETNGSTGVKNRL
                     TQEDIDNADGIIVAADKQVEMARFNGKKVVIVPVVQAMKRPKELINEALSGSAPVYSH
                     SGDSSNQSSGKTERRGFYKHLMNGVSNMLPFVVGGGILIAISFMFGIHSAEINDPTFS
                     PIAKLLKDIGGGNAFFLMIPVLAGFIGSSIADRPGFAPAMVGGLIAANSGSGFLGGLI
                     AGFLGGYVVVLLKKIFSKLPQSLEGIKPVLLYPLFGILITGALMYLVIVGPVTALNNN
                     IAIWLQNMGTGNLVLLGIVLGGMMAIDMGGPINKAAFTFGIAMIASGNYYPHAAIMAG
                     GMVPPLATALATTFFKNKFTKDERDAGTTNYIMGLTFITEGAIPFAAADPFRVIPACV
                     IGSALAGGLSMVFKIGLPAPHGGIFVIPVVEGNPLLYLLSILIGSIVAALIIGFLKKP
                     VNEINK"
     gene            complement(8363..9361)
                     /locus_tag="K8M77_001759"
     CDS             complement(8363..9361)
                     /locus_tag="K8M77_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HBI7086251.1"
                     /translation="MSSTLAVTVVIILLTLAFDFINGFHDTATAVATSISTRALSPKA
                     AIALCAIFNFIGAFLGTEVAKTVGDGIVDQNKIPLWVIAAVILGAIIWNLITWYFGIP
                     SSSSHALIGSLIGGGIAYSHSINIVSWGTLFDEVILWLILSPIIGLIVGYIFMILLNW
                     ALRRCKPGPVNRIFSKLQVVSGALMALNHGGNDAQKSMGIITMALLSGGFLSTFEVPT
                     WVIALCAIAMATGTSIGGQKIIKTMGSGVAKLNAVNGFAAQTGAAGVIGIATMFHAPV
                     STTHIITTTIMGVGASKNPKAVKWGVAKNIVWAWILTIPISACIAAIVVEIFKFIL"
     gene            complement(9354..9986)
                     /locus_tag="K8M77_001760"
     CDS             complement(9354..9986)
                     /locus_tag="K8M77_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HBI7086252.1"
                     /translation="MFNLNPKDDKFYDMFIDEAKNVEKAAVILSEALGDLENKTSHVA
                     RVEELEHKGDEIVHRLIEELNNSFITPIDREDIFSITKRMDDILDSIESTMHRFTMFN
                     INESKEGAKVLGSLIVDASRELVSLMEEFKHMKKKNKEITERVISINKIENKGDIFYR
                     QMISELFDEDGKTPVLDIIKWREIYKFLEATLDSCEAVANIVEGVVMKYV"
BASE COUNT         3587 a         1509 c         1324 g         3653 t
ORIGIN      
        1 aattaagttt attttaaaac aaagtagctt ttaatttata tcaaattcta aaagtgtatt
       61 ttttgtgaat ttatttgcct tttattaact tatagtattc tttagagaat tttcaaagaa
      121 tactataagc tatttataaa aataccctta taaaaaatta gaaagtgact tatttaattg
      181 actatagttt ttcctcttcc cttttactca atgatgcaag ggttgaatca tgagaaaaac
      241 ttattaaatt actcttactt actcctattt tttgagaagt atatattccg gtatgtcctg
      301 agaagatata acttattgcg cacgccataa ataaatattg tgcacctcct actccaaaca
      361 tctctattcc taaaacaaaa gaagcaatag gcgtattagt tgctcctgca aatacaccta
      421 ctaaccctaa agaagctaaa aatgaaggtg ataaatttaa tattcctgat aatgtatttc
      481 ctaaggttga tccaataaca aataaaggcg ttacttctcc cccttgatat ccagcaccta
      541 aagttattga tgtaaaaata agtttcccta aaaagtcaaa aggtgatata tgtccattga
      601 aagcttctga taataaagga agacttaaac ctaaatattt tctacttcct actaaataaa
      661 ctaataatat tacaatacat cctcctacaa agctttttaa tgaggtattc ttaaatttat
      721 tagaaaatac tttttttaat ttatgtgtaa gttcactaaa taatctacta gctagtccaa
      781 aacaaattgc tgaaattatt acttttaaaa taataagagc tgagatatct ggtactccta
      841 aaaccttata atgagaatga tgcactccta aatttttaac aacttcattt cctacaatac
      901 tagcaaagaa acttggtatt aaagcttcat aactcatttt acctaataca gagacttcta
      961 atccaaatat agttccagca agtggtgtac caaatatagc tgaaaatcct ccacttaccc
     1021 ctgccataag cattatcttt gaatcttcct tatttaattt caataacttt cctatagtct
     1081 ctgctatact tgcaccaatc tgtactccag ttccttccct acctgctgat cctccaaaaa
     1141 agtgagttac aactgttcca aagaaaacta aaggaaccat acgaagtggt acatctccac
     1201 aataattatt tatattttca ataattaaat tattaccttt tactgagttc cttccaaact
     1261 tatagtatag aaagcttact aaagcccctc caataggaag taaaaataaa acaaagccat
     1321 gattaactct gtaatcagtt gcccattcta aagattttaa aaatatagct attactcctc
     1381 ctataaaaat tcctattaaa cttcctatta atagccactt taaaaatgtc tttcctagaa
     1441 gaaaagtatt attgctacta atatacttta aatatttctt agatttttgc ttcatttata
     1501 ttctcccttc attaatatta ataatttttg agcaaaaaat aactcctaca aaatacaagc
     1561 tatataaaca tataacttgt attttgtagg agtcattagc ataaagcatt taatggcgaa
     1621 ctccatcgcc tataaaaatt actttaaaat agtaacataa ttataaataa ttgtccatat
     1681 tcaatttcaa cttataaaaa acttataatc acttaaacct acttatatta attcactaaa
     1741 gaaattatat ttttaatcta aaataatatc ttcaataaca ttcacaccat tatcaatcat
     1801 agagtttaaa aatacataat tcataaaatt agctttatgc ataggcatat caaatgtatc
     1861 tacaagtttt ttgggtacat atatttcccc atctttatta actctattaa attctgctct
     1921 taagcttata gctaattgat aaatgcatat atctgtacaa tctcctacta ttattacatt
     1981 gtctatctct tctttattta atatgaaatt ctcctctaaa aatccatttg ttgaattttt
     2041 ctttataact tctattccct ctaagttcaa ttcctcaaca agttcccatt catctgtatc
     2101 cttcatacaa tgcttaggat atgcttttaa ttctattgag tcttctgtat gataatcagt
     2161 aaaggcttta acttttattc cattatttaa agcatacttt gctaaattac ttattggatt
     2221 tattaatttc tctattctat cactatataa agctcctgct ttagcaaatc ctttatttat
     2281 atcaactatt aaaagtattg ttctattctt atctaaactt tttaaatcta aactttctaa
     2341 ggattgaatc ctttcataaa ttccacttat catttcatta ctttcactta ataatagttc
     2401 tttagtatcc acgatttaaa tccccctata tttttttata tttattttat tatgtaacca
     2461 attcatatct ttatcaataa ttatagatat ttttaacatt taattaaaga attgattctt
     2521 aagaaatttt taaaaattac attaaataat ttcctttatt atatttatat aacatgaact
     2581 ctcatttgat aaattgattt taaaaccata taatcaaata gtaatttata ttattaaata
     2641 ttaaatctta tagagaagga gggtacatat tgaataattt ttttagaaac aataaatcat
     2701 atataaaaat ggtattagta atagtagtat cttatctatc aataaagtta atagataata
     2761 ttcctagtgt tatgtctgct attcatacaa ttttagcagt actattacct tttttattag
     2821 catttattat tgcctatata ttaaatccaa ttgtaaattt atttagcaaa aaatttaaat
     2881 tatcaagagg aattagtata gtaataactt acttaatatt tgtaacatca atttcactag
     2941 cagcaactta tttattgcct aaattatata caagtttaat tgagttaata gataatatac
     3001 ctgcatttac taattatgta caaaacttat ttacagaaat aatggcaagc tttaaagttc
     3061 ctgaaggaat aaactcttat ttacctcatg gagatactaa taaactaata acatttattg
     3121 gttctatggt tacattatta tcaaactggt tattagatgc tgccataagc ttaactagtt
     3181 ctataataaa ctggatattt ggattcttaa tttccatata tgttttagta gataaagata
     3241 aatttttagc tcatagtagt aaagttacaa gaatcatttt tggtaaaaat cttggagacg
     3301 aaatattaga atttataaag atacttaatg aaaaaatagg aacttatgtt ggaataaaag
     3361 caatagattc cttaataata ggtttactag catttgttgg attaacagtt ataaaatctg
     3421 agtatgcact tttattatca gtattagttg gtataactaa tatgattcct tactttggac
     3481 catttatagg tatggttgta ggatttttta taaatgtatt tataagccca atgaaagcag
     3541 ttattgtttt aatatatctt ttcatattac aacaatttga tgcttggtac ctagatccaa
     3601 aacttatagg aaacagagtt ggattaagtc catttctagt aattcttgga gttactcttg
     3661 gaggagctat ttatggtcct ataggtatga tacttggaag tccagttatg tcagttatta
     3721 gaatctatac tttaaaatta attaaaaaat ttgaatatag agcagaataa gaaaaagaag
     3781 ttgtattaaa gcaacttctt tttcttaatt aaatatattt taaatcaaat ttaatttcat
     3841 tatatattca tttaaaacta tatttttatt ttttgatttt acaattttta atattgaatg
     3901 agattttttt attgctacag taattgacat atcacagtca ctctcctttt caaaatacat
     3961 aagtatttgg ccagttaaaa cttctccccc aatatcaact ataggctcaa tagcttttat
     4021 attattattt atatgagtgc ttactattat attaactccc tctttcatcc caatctcaat
     4081 aaatccataa tcctttgaaa tgcaaataac tttcccatta actggagaaa ataaaaattc
     4141 attatgtgaa tttaaactaa ttatttcttc taaatcctct aaattaaaag ccttgtttaa
     4201 aacaatataa tctaaaacat ttccctcacc tggcgccata atataaattt cattatctga
     4261 tttatttaac atatataaac acctcttgtt tttaaaatat ttagacctta ttaatttcta
     4321 tgttttcagc ttttaaatta taattctatc tacacttatc ttaattaaag aatatcttaa
     4381 taaattatct ttttccttta taataagtta tagaatatat aattcataaa aagccaaatt
     4441 caatcacaaa atcttttata attaaaattt ttgatataat accttgtaat ttttgaatgt
     4501 tttttattga ttttgattgt ttttggatgt ataatttagt tacaaggttt taggaggtta
     4561 ttatgttaag tgaaaaaaga catgagataa tattaaactt attaaaacta aagggttttg
     4621 ttagcctagc tgaattactt gaagctactg aaagctctga atcaactata agaagagatt
     4681 taagttattt agaatctata aatctattaa aaagagttca tggtggtgct aaatcattga
     4741 ctaatgtttc aaaagaatta agttacaatg agaaatcatc caaaagcatt cacgaaaaaa
     4801 gagctatagc aaaatatgca gcttctttaa tagaagatgg cgattgtatt tttatagatg
     4861 caggcactac aacatatgaa ttaatcgatt acttagaggg aaaagatata cttgttgtaa
     4921 gtaatggctt atctcatata gatacattaa tacaaaaaaa tattaagtgc tatgtaatag
     4981 gtggaaatat taaaatttct acaaaagcaa taactggatg tgatgcatta atgtgtcttt
     5041 caaaatttag attcgataaa tgctttattg gagctaatgg agttcatcat atctatggac
     5101 ttactactcc agatactgaa gaagcaatat taaaagaatg cgcaataaat aattctagaa
     5161 aatcttatgt attagctgac gactctaaat ttggagagat ctcaacaata aaattctctg
     5221 atattaataa atgtgtaata ataacaaacg aaaaacctaa agataatgca tatgataaaa
     5281 aaactgatat aaaggtggtt gatatttaat gatttatact cttactctta atccatctat
     5341 tgattatgta atagatgtag aaaacttaga attaggaaaa gtaaatagaa caagtaaaga
     5401 ttttaaattt cctggcggaa aaggaattaa tgtttcaaga attttaggaa atcaaaaaat
     5461 agataatata gctcttggat tccttggtgg atttactggt aagttcatcg aagataatct
     5521 aaaggcttta ggggtaaaaa cagaatttat aaaagtaaaa ggtgattcaa gaattaatgt
     5581 aaaaattaaa tctaatgaag aaacagaaat aaacggaact ggtccaatga tagcatctga
     5641 agaattagag gcactattta atagattgga tcaattagta gaaggagata tattagttct
     5701 tgcaggtagc attccatcta ctcttcctaa agatttatac cttcaaatac aaaagagagt
     5761 ttctaagaaa aatgtaaagg ttgtagttga tacttctgga aaggctcttt tagaagctat
     5821 aaaaaataaa ccttttttaa taaagccaaa taatcatgaa attgaagaaa tatttaatgt
     5881 aaaaataaat tctgaaaaag aacttattaa atatggaaaa aagttagttt ctcttggagc
     5941 agaaaacgta ataatctcta tggctggaga tggtgcttta ttaataacta aggatggtgt
     6001 atttaaagga aatgcaccta aaggagaagt taaaaattct gtaggagctg gcgactcatt
     6061 agtaggaggt ttcttagcaa aatatgttga aactaaagat ttaattgctt ccttcaaaaa
     6121 tggaatagcc tcaggaagtg ctagtgcatt ttctttagat ttagctcttg ctgaagatgt
     6181 caataaatta ttaccaatta taaaaattga aaaatatgag gaggtttaaa atatgaaaat
     6241 ttctcaatta ttaagtcttg acacaataaa actagacctt gctgcatcta ataaaaatga
     6301 agttatagac caattagttg atgttttgtg ggatgccgga aagttaactg ataaaaatct
     6361 ttataaagaa gaaattttaa aaagagaaag cttatcttcc actggtattg gcgaaagaat
     6421 agcaattcct cacgctaaaa caaaggcagt aaaaaatgca tctttagctc ttggtcttta
     6481 taaagatgga gtagattatg attcacttga tggtgaacct gttaatatag tatttatgat
     6541 tgctgctcca gatggagcaa atgatgagca tttagaaact ctatctagac tttccgtact
     6601 tttaatgaat cctgatttca aaaatggact tttaaatgct aaaacaccag aagaagttat
     6661 ttctcttctt gataaatttg aagaaaatga agaatctgat gaaattaata attctaacga
     6721 aatagaaaat aattcaaata aaaaatatat cttagctgtc acagcatgtc caactggtat
     6781 tgctcatact tacatggctg ctgattcact taacaaaact gctaaggaaa tggatataaa
     6841 tataaaagtt gaaactaatg gttcaacagg tgtaaaaaat agattaactc aagaagacat
     6901 agataatgct gatggtataa tagttgcagc cgataaacaa gttgaaatgg ctagatttaa
     6961 tggtaaaaag gttgtaatag ttcctgtagt tcaagctatg aaaagaccta aagaattaat
     7021 aaacgaagca ttaagtggct ctgctccagt ttattctcat tctggagatt cctctaatca
     7081 atcttctggc aagactgaaa gaagaggatt ttataaacac ttaatgaatg gtgtttcaaa
     7141 tatgttacca tttgttgttg gtgggggtat attaatagct atatcattta tgtttggtat
     7201 tcactcagct gaaataaatg atcctacttt tagtccaata gccaaattat taaaagatat
     7261 tggtggagga aatgctttct tccttatgat tcctgttctt gctggattca ttggatcaag
     7321 tatagctgat agacctggat ttgcacctgc tatggttggt ggtttaatcg ctgctaacag
     7381 tggttctggc ttccttggtg gattaatagc tggtttcctt ggtggatatg tagttgtatt
     7441 attaaagaaa atatttagta aattaccaca atcactagaa ggtattaaac ctgttttact
     7501 gtatcctcta tttggtatct taataactgg tgccttaatg tacttagtaa ttgtgggtcc
     7561 tgtaactgct ttaaataata acatagcaat atggttacaa aacatgggaa ctggaaactt
     7621 agttcttctt ggaatagtat taggtggaat gatggctata gatatgggag gtcctataaa
     7681 taaggctgca tttacctttg gtatagctat gatagcttct ggaaactact atccacatgc
     7741 tgctataatg gctggtggta tggtacctcc tttagctaca gctttagcaa ctactttctt
     7801 caaaaataaa tttactaaag atgaaagaga tgctggtact actaactata taatgggatt
     7861 aactttcata actgaaggag ctattccatt tgctgccgct gacccattca gagttatacc
     7921 tgcttgtgtt ataggttcag ctctagctgg aggattatca atggtattta aaataggttt
     7981 acctgctcct catggtggaa tattcgtaat tccagtagtt gaaggaaatc ctcttttata
     8041 tcttctatca atattaatag gctctattgt agctgctcta ataataggat tcttaaaaaa
     8101 acctgtaaat gaaataaata aataagttaa tatataaaat aagtcttaat aaaacttaca
     8161 aacatattcc gagaattact agatattctt ttcatactgt ttgtaagttt tatttttaac
     8221 tttttataac tactaagcag taagttaatc acctgaaaat aggtgatttt attttttccc
     8281 taaataaaaa gattagacag aatttctttt tagaatccta tctaatcttt aattaaaaat
     8341 ttctattata attactttta tactataata taaatttaaa tatttcaact actatagcag
     8401 ctatgcatgc acttattggg attgttaata tccaagccca aactatattc ttagcaactc
     8461 cccacttaac tgctttaggg tttttagaag ctccaactcc cattatggta gttgttatta
     8521 tgtgagttgt acttacaggt gcatggaaca tagtagctat ccctataact ccagctgccc
     8581 cagtttgtgc tgcaaatcca ttaacagcat ttaatttagc aactccactt cccattgttt
     8641 taattatttt ttgtccgcct attgaagttc ctgttgccat tgcaattgca cataaagcta
     8701 taacccaagt tggtacttca aaagtagata aaaatcctcc acttagaagt gccatagtaa
     8761 ttatacccat tgatttttga gcatcattac ccccatggtt taaggccatt agtgctcctg
     8821 aaaccacttg taattttgag aaaattctat ttaccgggcc tggtttgcat cttcttagtg
     8881 cccagtttaa taaaatcata aatatgtatc caactattaa acctataatt ggtgataata
     8941 taagccaaag aattacttca tcaaaaagag ttccccaact tactatgttt attgaatgtg
     9001 aataagctat tcctccacct attaatgatc ctattaaggc atgagaagaa ctacttggaa
     9061 ttccaaaata ccaagtaata agattccata taatagcccc taatataact gccgcaataa
     9121 cccatagagg tattttattt tgatctacta taccatctcc tacagtttta gcaacttcag
     9181 ttcctaaaaa tgctccaata aagttaaata tagcacataa agctatggca gccttaggtg
     9241 aaagagctct tgtggaaatt gatgtagcaa ctgccgttgc agtatcatga aatccattta
     9301 taaaatcgaa agctaatgtt aagagaataa taacaactgt tacagctaaa gtactagaca
     9361 tacttcatca ctactccctc aactatattg gctacggcct cacaactatc taaagttgcc
     9421 tctaagaatt tgtatatttc tctccatttt attatatcaa gaactggagt ttttccatct
     9481 tcatcaaata attctgatat catttgtcta tagaagatat ctcctttgtt ttctatttta
     9541 tttattgaaa ttactctctc tgtaatttct ttatttttct ttttcatatg cttaaattct
     9601 tccattagag atactaattc tcttgatgca tcaactatta agcttcctaa aactttagca
     9661 ccttctttag attcatttat attgaacata gtgaatctgt gcatagtact ttctatagaa
     9721 tctaaaatat catccattct ttttgttata gaaaatatat cttctctgtc aataggagtt
     9781 ataaaagaat tatttaactc ttctataagt ctatgaacta tttcatcgcc tttatgttct
     9841 aactcctcaa ctctagccac gtggcttgtt ttattttcaa gatcccctaa ggcttcgctt
     9901 aatattactg ccgctttttc tacatttttt gcttcatcga taaacatatc ataaaattta
     9961 tcgtctttag gatttaaatt aaacatttgt atttcatctc ctggttctca ttattaattt
    10021 ttattgaaca aatctagtat atcataatat ttttttaaat ttaacataca att
//
LOCUS       DAEVGI010000091         9715 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEVGI010000091 DAEVGI010000000
VERSION     DAEVGI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.091"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     misc_binding    12..271
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            460..1470
                     /locus_tag="K8M77_001761"
     CDS             460..1470
                     /locus_tag="K8M77_001761"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBI7086253.1"
                     /translation="MEKLFIPKDYKPLLSLRETEVAIKELKDFFEDSLAKNLNLTRVS
                     APLFVNKGSGLNDDLNGIERPVSFDMKAIPEFNIQIVHSLAKWKRLALHRYEFKHGEG
                     LYTDMNAIRRDEDLDNIHSIYVDQWDWEKIIDKEERNLETLKETVRSIYSTFKATEDF
                     IVSKYPHIEKILPEDITFITSQELEDRYPDLTSKERETAICKEFGAVFIIGIGGKLAS
                     GEKHDDRSPDYDDWTLNGDLLFYYPLFDEAVELSSMGIRVDEESLLKQLKIAECEERK
                     ELPFHQMLLEGKLPYTIGGGIGQSRICMFFLRKAHIGEVQASMWDEDMIRTCEENNIH
                     LL"
     gene            1596..2414
                     /locus_tag="K8M77_001762"
     CDS             1596..2414
                     /locus_tag="K8M77_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086254.1"
                     /translation="MKRSILNTLLNVLAIVFIVFLMIKVSVLTGSILLLSFIIFKLTI
                     NKHLIYMFKGAKKLRANNLEEALSLYRKAALCNSSNVKAIKTYVFLELKIGSYAEALE
                     TLKRIVSKRKFLPEDSNQLDLLQAILYWKLNDIKTSLQILDDLKANNFNSLDFYEVYG
                     YVLIQDEDFEKAISISNEGLKVDELSQIIRANLGEVFYKIGDIEKACFYFDELIDECV
                     NFSEPYYFMGIISKEKEDFYKAKELLNKALKYDESILSNLSKNDIENALISINH"
     gene            2526..2903
                     /locus_tag="K8M77_001763"
     CDS             2526..2903
                     /locus_tag="K8M77_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBI7086255.1"
                     /translation="MKLLREMLIIVFIYFLGEFISKSFIHSVPGNIIGMLILLVLLCT
                     KVVKVEAIDCVAQFFLDHLAFFFVPAGVGLMTSVGLLEGNVLKLLFICIASTFVVIGV
                     TGLVVQALIRRKEKKLLGDEVNE"
     gene            2896..3606
                     /locus_tag="K8M77_001764"
     CDS             2896..3606
                     /locus_tag="K8M77_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBI7086256.1"
                     /translation="MNNLVNNPLFGILLTLFAFEIGMTIYKKTKIPLLNPLLIAIALV
                     IAVLVSCHISFETYNIGGNFINAFLGPATVILAVPLYKQLQLLKNHFVPIMTGIIVGS
                     FASMCCVIIMGYFLGVNHELIASLVPKSVTTPIGVEVSSGLGGIPSITVIAVIITGIT
                     GAIIAPMVCKLLKIDHEVAMGVSMGTAAHAVGTTKALEIGETHGAMSSLSIGVAGLIT
                     VFLAPPIYMLSALILKFV"
     gene            3704..4159
                     /locus_tag="K8M77_001765"
     CDS             3704..4159
                     /locus_tag="K8M77_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086257.1"
                     /translation="MKIYKKILGFFALAVLLNPNTLTLDASTFSKEDQIQNKEILYDI
                     TAFTTEKNTLNLIINQLFKEDNTLFIDAYFLNTTENTTFENLENFNISVYDTNNKEVL
                     NKTFKSVVIPKGLPPQHGRRILFPIENEFFNLQEKDLSKLSYKFTFDYN"
     gene            complement(4227..6389)
                     /locus_tag="K8M77_001766"
     CDS             complement(4227..6389)
                     /locus_tag="K8M77_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBI7086258.1"
                     /translation="MKKVIIAEKPSVARNIADAFNIKKRKDGYFEGEEYLITWAFGHL
                     LQLFDAKDYDENMKGWRMEKFPFIPEVFRYKIKQDSLNRNIIDKGAEKQIKIIKGLID
                     REDVDGIISATDFDREGQVIADEIFLYFSEKKPVYRLLLNEWTPDEVKKGMEKLKPNE
                     EMQSLQDAGIGRQWADWIIGINLTSVTTIKYKLNDGQILNIGRVLLPTLKIIYDRDKE
                     IENFKSSAYYKLNGTFKTSKNEEFEGTYYENNSEKFEDKKVLDEIKEKIKDKNAEIIE
                     KQTERKKEYPPFLFNLSNLQGYITSKYKGWTSDKVLKVAQSLYEKKFITYPRTGSMAL
                     EESLVDRTRNVLEKLKVGLPYEEQIKFVQNKRVFDNSKVESHSAIVPTYMKPNNLTQD
                     ERIVYTAVKNRFIMQFMPVAEFEETKITSKVNDKEVKGVFISKGKVQLVEGWRVVEKI
                     DSKDTILPMVNEKEVVDLVDSKINKVTKKPPKKHTEKTLLRVMETCGKSFKEDAEEDS
                     EEMMASILSGFSIGTPATRAETIKKLKDVGYITTKGKSLTCTELGRTIVEIFPVKELL
                     DLEYTGRLEKTLSDIERGKVKKEDFLNLIKNFTIEGVKAIKNDDSMAKHFKVEAPEGV
                     EVLGKCPNCGNPVIEGERGFGCVNWKNGCKYTIWKNDKYIASFGKQVTPQMVKLLLAN
                     GKVGFRNLKSKKGNTFAAYFIYEKDEEKGYFTWKMEFI"
     gene            complement(6618..7775)
                     /locus_tag="K8M77_001767"
     CDS             complement(6618..7775)
                     /locus_tag="K8M77_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBI7086259.1"
                     /translation="MLIKNGKIFTCEEGKIYEKGDILIKDGKISRIGEDLSQYIGEEE
                     VIDAKGLLIFPGFIEAHCHLGLHEEGNNGAGNGTNEASEPITPQMRAIDGINPFDGGF
                     QSAREAGVTTAVIGPGSANVIGGQFAAVKTSGICIDDMIIKEPVAIKVAFGENPKRVY
                     SGKNKMPNTRMAIAALLRETLTEAVNYKNRKIDAEIEDRDFSKNLKYEALLPLINKEI
                     PMKAHAHRADDILTAIRIAKEFNLKLTLDHCTEGDLISDYIKRENLDAIVGPTLSFNG
                     KAETLNKTFKTPKALIDKGIKVAITTDHPVVTIDNLPLCAAMAMKEGITFNEALEAIT
                     INPAKIIGIDERVGSLKEGKDGDLVILNGSPFEIATKTIYTIINGEVVYKD"
     gene            complement(7938..8435)
                     /locus_tag="K8M77_001768"
     CDS             complement(7938..8435)
                     /locus_tag="K8M77_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBI7086260.1"
                     /translation="MNIFKVNGKFNLGDLIISLLITLGGGYVIGKFIKESVQVYSTLN
                     KPWFSPPAIVFPIVWTILYILMAIAAYRIFLRHKQGEKTKSALWFYIIQLVLNFAWSI
                     IFFNFKLYGLAFIELIILLIFIVITTIKFLKFDKIAGILMIPYIIWVSFAGVLNFFVW
                     MLNEM"
     gene            complement(8481..>9715)
                     /locus_tag="K8M77_001769"
     CDS             complement(8481..>9715)
                     /locus_tag="K8M77_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7086261.1"
                     /translation="EFDRNNRYLRKKIEELKELENLLDREELLFNKINSAIGDYIFIS
                     KGEKLFYLNDEALEFLEVENKESILFESMEVLYEKILEKQVYLKEKGSMDSYKEIIFK
                     NIKGGYSNGEFYTIPFGDNYKIIIINDITKKNNALRLNKYLKYKLEEENIKGEFFTNI
                     CHELRTPINVINSALQLNNLNIENRDINSIERNNLVIKQNCLRLIRTINNFIDANKIS
                     EGQIETNIMVLNVVEVVENILDASSEYISKKKINFIFDPDFEEIFIAIDSEFLERIIL
                     NILSNSVKYGSENGNIYVKIYFEGKDLVIMIENDGVAISYDEQKYIFDKFTKSNKALN
                     RTQEGSGLGLYISKSLMKMQGGDLKVDIYEGHGNRFKLYFYDVDLFKEIDRTEHIFNN
                     NYYNLKERTEIEFSDIYI"
BASE COUNT         3244 a         1527 c         1161 g         3783 t
ORIGIN      
        1 taaataagtt gtacaaagca ttgatgggaa aaagtaatct ttggaaagag ttttagcgat
       61 tgagggatgg tgaaagcctc atactatacc tttgatgaag aacatccttg agcttctaac
      121 cgaacttcaa aatttatttt ggacagtaga cttagacggg tttctttccg ttaaaagaag
      181 gacagtgtta agatgcattg ttattaagtg agtgcttttg tactaacata ggtggtaccg
      241 cggagatgat tatacagcct tcgtcctatt aaaaatatag gatgatggct ttttttattg
      301 tctttttata atatattttt aaattttatt attttaacta tacgtataaa tcaaaacatt
      361 taaagaaatt ttaataacct gtttggggcc aaacaaattt ataaaaaaat atatgtagca
      421 aaagatatat ctacatttaa aattaaggag ttgtttaaaa tggaaaaatt atttatacca
      481 aaggactata aacctttatt atcacttaga gaaacagagg ttgctattaa agaacttaaa
      541 gactttttcg aagattcttt agctaaaaac ttaaacttaa caagagtttc tgctccactt
      601 ttcgtaaata aaggttcagg attaaatgat gatctaaatg gaatcgaaag acctgtaagt
      661 tttgatatga aagctatacc agagtttaat attcaaatag ttcattctct agctaaatgg
      721 aaaagactag ctcttcatag atatgaattt aaacatggtg aaggtcttta cactgatatg
      781 aacgctataa gaagagatga agatttagat aatatccatt ctatttatgt tgaccagtgg
      841 gattgggaaa aaataataga taaagaggaa agaaatttag aaactttaaa agagactgta
      901 agatcaatat atagtacttt taaagcaaca gaagatttta tagtatcaaa ataccctcac
      961 atagaaaaaa ttcttcctga agatataaca tttataacat cacaggaatt agaagatcgt
     1021 tatccagatc ttacttctaa agagagagaa actgctatat gtaaagaatt tggagctgta
     1081 ttcataatag gaattggtgg aaaattagct tctggtgaaa aacatgatga tagaagtcca
     1141 gactatgatg attggacatt aaatggagat ttattattct actatcctct atttgatgag
     1201 gctgtagaac tttcatcaat gggaataaga gttgatgagg aaagcctttt aaaacaatta
     1261 aaaatagcag aatgtgagga aagaaaagaa ctaccattcc atcaaatgct tcttgaagga
     1321 aaattaccgt atactatagg tggtggaatt ggtcaatcta gaatatgtat gttcttcctt
     1381 agaaaagctc acattggaga agttcaagca tctatgtggg atgaagatat gataagaact
     1441 tgtgaagaaa acaacataca tttattataa aagttatatt gaaattttga tttttattta
     1501 atttaacttt aactttaaaa aattcaatat tatccctctt ataaatatag tataatttct
     1561 ttaataaact aatatttaaa agggggataa ttattatgaa aaggtctatt ttaaatacat
     1621 tattgaatgt ccttgcaata gtttttatag ttttcttaat gattaaggta agtgtgctta
     1681 cggggagcat actactttta tcatttataa tatttaaatt aactataaat aagcatttaa
     1741 tttatatgtt taagggtgct aaaaaactta gggctaataa tttagaagaa gctttatctc
     1801 tttatagaaa ggctgctcta tgtaatagtt ctaatgtaaa agctataaaa acttatgttt
     1861 tcttagaact taaaatagga tcttatgctg aagctttaga aactttaaaa cgcatagtat
     1921 ctaaaagaaa atttttacct gaagattcaa atcaacttga tttgcttcaa gcaatacttt
     1981 attggaaatt aaatgatata aaaacatctc ttcaaatctt agatgattta aaagctaata
     2041 attttaactc tctagatttt tatgaagttt atggatatgt tttgattcaa gatgaggatt
     2101 ttgaaaaagc aatttcaata agtaatgaag gattaaaagt tgatgaattg agccaaataa
     2161 taagagctaa tttaggagag gttttctata aaattgggga tatagaaaaa gcttgctttt
     2221 attttgatga attaatagat gaatgtgtaa atttttcaga accttattat ttcatgggaa
     2281 ttatatcaaa agaaaaagaa gatttttata aagctaaaga acttttaaat aaagcactta
     2341 agtatgatga gtctattcta agcaacctat caaaaaatga tatagaaaac gctttaataa
     2401 gtataaatca ttaattattg tgatatttga ttatagcttt aattttaaat tcattctata
     2461 atgtataagg aactttaaaa attgttcctt atattttttt gataaaaaaa ggaagtgatt
     2521 atctagtgaa gcttttaaga gaaatgctta ttatagtatt tatatacttc ttaggcgaat
     2581 ttatttctaa aagttttata catagtgtac ctggaaatat tattggaatg ttaatattat
     2641 tagttttact atgtactaaa gttgttaaag tagaagctat tgactgtgtt gctcaattct
     2701 tcttagatca tttagccttc ttcttcgttc ctgcaggggt tggacttatg acatctgttg
     2761 gattattaga aggaaatgtt cttaaattat tatttatttg tatagcctca acttttgttg
     2821 taataggagt tacaggttta gttgttcaag ctttaataag aagaaaagaa aaaaaactac
     2881 taggagatga agttaatgaa taaccttgtt aataaccctt tattcggaat tttattaact
     2941 ctttttgcat tcgaaattgg tatgaccata tacaagaaga ctaaaattcc cctactaaac
     3001 cctttattaa tagcaattgc tcttgtaatt gcagttttag tttcatgcca tataagtttt
     3061 gaaacttata atattggtgg taattttata aatgcatttt tgggacctgc aactgtaatt
     3121 ctagcagtac ctttatataa acagttacaa cttttaaaaa atcattttgt accgataatg
     3181 acaggaataa tagttggaag ttttgcttct atgtgctgtg ttataataat gggatatttc
     3241 ttaggagtaa atcatgagct tattgcttct ttagttccta aatcagttac tacaccaatt
     3301 ggggttgaag tatctagcgg acttggtgga ataccttcaa tcacagttat agcagtaata
     3361 ataactggta tcacaggtgc aatcatagct cctatggtat gtaaattact taagattgat
     3421 catgaagttg ctatgggagt ttctatggga acagctgccc atgccgttgg aactactaaa
     3481 gctttagaaa tcggtgaaac tcatggtgct atgagttcat tatctatagg tgttgctgga
     3541 ttaataacag ttttccttgc tccaccaata tatatgctat ctgcattaat attaaaattt
     3601 gtctaaacag tgataaaatt tcactgtttt tttcttttta tgactatatt ttcttaatta
     3661 tagaaatact aaaaatatag atctataatt aaggagaatt aaaatgaaaa tatataaaaa
     3721 aattctaggc ttttttgcct tagcagttct attaaatcca aatactctaa ccttagatgc
     3781 aagcactttt tctaaagaag atcaaattca aaacaaagaa attttatatg atattacagc
     3841 cttcaccaca gaaaaaaaca ctcttaactt aataataaat caattgttta aagaagataa
     3901 tactctattt atagatgctt attttttaaa tacaactgaa aacactactt ttgaaaactt
     3961 agaaaatttt aatatctcag tttatgatac caataataaa gaggtactaa ataaaacctt
     4021 taaaagtgtt gttatcccta agggacttcc accacaacat ggtagaagaa ttttatttcc
     4081 aatagaaaat gagtttttta atctacaaga aaaagattta tcaaaattat cttacaaatt
     4141 cacctttgat tacaattaaa attttaaaac cttctcttaa ccaaataaaa aggagttgtt
     4201 ttaatacaac tccaaaatat attttattaa ataaattcca ttttccatgt gaaataacct
     4261 ttttcttcat ctttttcata tatgaagtat gctgcaaagg tatttccctt tttacttttt
     4321 aaatttctaa atccgacttt tccattagct aatagaagtt ttaccatctg aggtgttact
     4381 tgttttccaa aggaagctat atacttgtca tttttccata tggtatattt acatccattt
     4441 ttccagttaa cacatccaaa tcctctttct ccttctataa caggatttcc acagttagga
     4501 cattttccta aaacctctac tccctctggt gcctctactt taaagtgttt tgccatggaa
     4561 tcatcatttt ttatagcctt aactccctct atagtaaagt tttttataag atttaagaaa
     4621 tcttcttttt taaccttccc tctttctata tctgaaaggg ttttttctag tcttcctgtg
     4681 tattctaaat ctaaaagttc cttaacaggg aatatttcaa caattgttct accaagctct
     4741 gtacaagtta aactttttcc ctttgtagta atatatccta catccttaag ttttttaatt
     4801 gtttcagctc tagtagcagg tgttcctata ctaaatccac ttagtatcga agccatcatt
     4861 tcttcactat cttcctctgc atcctcttta aaactcttgc cacaagtctc cataacccta
     4921 agtaaagtct tctcagtatg ctttttaggt ggttttttag taaccttatt tattttacta
     4981 tctactaagt ctactacttc tttttcattt accattggaa gaattgtatc tttactatct
     5041 attttttcaa caactctcca tccttctaca agctgaactt ttccctttga aataaataca
     5101 cccttaacct ctttatcatt taccttggaa gttatttttg tttcctcaaa ttcagctaca
     5161 ggcataaatt gcataataaa tctattttta actgctgtat aaactattct ctcatcttga
     5221 gttaaattat taggtttcat gtaagttgga actatggcac tatggctctc aacctttgaa
     5281 ttatcaaaaa cccttttatt ttgaacaaac tttatttgct cctcataagg taatcctacc
     5341 ttaagctttt ctaaaacatt tctagttcta tcaactaagc tctcctctaa agccatactt
     5401 cctgttctag gataagttat aaacttcttt tcatataaag attgagctac ttttaaaacc
     5461 ttatctgaag tccatccttt gtatttactt gttatatatc cttgaagatt agaaagatta
     5521 aataaaaatg gtggatattc tttttttctt tctgtttgct tttcaattat ttcagcattt
     5581 ttatccttta tcttttcttt aatctcatct aaaacctttt tatcttcaaa cttctctgaa
     5641 ttattttcat aataagttcc ttcaaactcc tcattttttg aagtcttaaa agtaccatta
     5701 agtttgtaat atgcacttga tttaaagttc tctatttctt tatctctgtc ataaattatt
     5761 tttaaagtag gtagaagaac tctccctata tttaaaatct gtccatcatt tagcttatat
     5821 tttattgtag taacagaagt taaatttata cctattatcc agtctgccca ctgtcttcct
     5881 ataccagcat cttgaaggga ttgcatttct tcattaggtt ttaacttttc cattcctttt
     5941 ttaacttcat ctggtgtcca ttcatttaat aaaagtctat agacaggttt cttttcacta
     6001 aaatatagga aaatttcatc tgcaattact tgtccctctc tatcaaagtc agtagcagaa
     6061 attattccat ctacatcttc tctatctata agacctttta ttatctttat ttgcttttcc
     6121 gcacccttat caatgatgtt tctatttaaa gaatcttgtt ttattttata tctaaaaact
     6181 tctgggataa aagggaattt ttccattctc caccccttca tgttctcatc ataatccttt
     6241 gcatcaaata actgcaataa gtgtccgaaa gcccatgtta taagatattc ttcgccttca
     6301 aagtatccat cttttctctt ttttatatta aaagcatctg caatatttct agcaacagat
     6361 ggtttttctg caattattac ctttttcaaa gtatcactcc taattataat ccttttgtta
     6421 tattatctcc tcttctatat ctttgacaga tttaaaaatt tatttcactt attaaaataa
     6481 taagggctgt ctcaaaactt agcatttaat tataagtttt actaagttat attgatacag
     6541 ctccattaat ttatctaaaa tatattaaaa aatcaaattc aaacattatt tattataata
     6601 taaaattgct tttattccta gtctttataa actacctctc catttataat tgtataaata
     6661 gtttttgtag ctatttcaaa aggacttcca tttaaaatta ctaaatctcc atcctttcct
     6721 tcctttaagc ttccaactct ttcatcaata cctattattt tagctggatt tattgttatt
     6781 gcttctaagg cttcattaaa agtaattcct tctttcatag ccatagctgc acatagtgga
     6841 agattgtcta ttgttactac tggatggtct gtagttattg ctactttaat tcctttatct
     6901 attaaggcct ttggagtctt aaaggtttta tttaaagtct ctgcctttcc attaaaactt
     6961 aaagttggtc caactatagc atctaagttt tctcttttaa tataatcact tatcaaatct
     7021 ccttctgtac aatgatctaa agttaattta agattaaatt ccttagctat tcttatggca
     7081 gttaaaatat catctgctct atgggcatga gctttcatag gtatttcttt gttaattagt
     7141 ggaagtaaag cctcatattt taaattctta ctaaaatccc tatcctctat ttcagcatca
     7201 atttttctat ttttataatt aacagcctct gttaaagttt ctcttaataa agctgcaata
     7261 gccattcttg tattaggcat tttattcttt ccagaataaa ccctttttgg attttctcca
     7321 aaggcaacct ttattgctac aggttccttt attatcatat catcaataca tattccactt
     7381 gtttttacag cggcaaactg tcctcctatt acattagcgc ttccaggccc aattacagct
     7441 gtggtaaccc ctgcttccct tgcagattgg aatcctccat caaaaggatt tattccatct
     7501 atagctctca tttgtggggt tataggttca ctagcttcat tggttccatt tcctgcccca
     7561 ttatttcctt cttcatgtag tcctaaatga caatgtgctt caataaaccc tggaaatatt
     7621 aatagtcctt tagcatcaat aacctcttct tctcctatgt attgacttaa atcttcccca
     7681 attctactta tctttccatc cttaattaga atatcacctt tttcatatat cttaccttct
     7741 tcacaggtaa atattttccc atttttaatt aacatttttt atcccccttt tatttatgtg
     7801 atttaataaa tattaccttt agctattctt taaatcatac tttattttaa agaaagtttt
     7861 tattaaatat ctctatataa ttatagcact aaataaaaaa tccctcttat tctattaaaa
     7921 taagagagat ttttaaatta catttcattt aacatccata caaaaaagtt taatactcca
     7981 gcaaaggaaa cccatatgat ataaggaatc ataagaattc ctgctatttt atcaaacttt
     8041 aaaaatttaa ttgtggttat aactataaat attaataata tgatgagttc tataaatgct
     8101 aatccgtata acttaaagtt aaaaaatatt atgctccaag caaaatttaa tacaagttgt
     8161 attatataaa accaaagggc tgactttgtt ttttctcctt gcttatgcct taaaaatatt
     8221 ctgtaggctg ctattgccat aagtatatat aaaatagtcc atactatggg aaaaactatg
     8281 gctggtggac taaaccaagg tttatttaaa gtgctatata cttgtacact ctcctttata
     8341 aactttccaa taacatatcc tccacctaaa gttattaaca aacttattat taaatcacct
     8401 aaattaaatt ttccattaac cttaaatata ttcacataaa tcacctctta ggtaattata
     8461 tgtattcctt ttgatatata ttatatgtat atatcagaaa attcaatttc agttctttcc
     8521 tttaagttat agtaattgtt attaaaaata tgttcagttc tatcaatttc cttaaataaa
     8581 tctacatcat aaaaatataa tttaaatcta tttccatgtc cctcatatat atcaaccttt
     8641 aaatctcccc cttgcatttt cataagggat ttacttatat aaagccctaa tccacttcct
     8701 tcttgtgttc tatttaaagc cttattactt ttagtaaatt tgtcaaaaat atatttttgt
     8761 tcatcataac ttatagctac tccatcattt tctatcatta ttacaagatc ttttccttca
     8821 aaatatattt ttacatatat gtttccattt tcacttccat actttactga atttgataat
     8881 atatttaata ttattctttc taaaaactca ctatcaatag ctatgaatat ttcttcaaaa
     8941 tcaggatcaa aaataaaatt tatttttttc ttacttatat attctgaaga agcatctaat
     9001 atattttcaa caacttcaac cacattaaga accataatat ttgtttctat ctgcccctct
     9061 gatattttat tagcatcaat gaaattattt attgtcctta taagtcttaa acaattttgt
     9121 ttaattacta gattatttct ttctatagaa ttaatatctc tattttctat atttaaatta
     9181 tttaattgaa gagctgaatt tatcacattt ataggtgttc ttaattcgtg acatatattt
     9241 gtaaaaaatt ctccttttat attttcttcc tctaatttat attttaaata cttgtttaac
     9301 cttagggcat tattcttttt agtgatatca tttataatta taattttata attatctcca
     9361 aagggtatgg tataaaattc tccatttgaa taacctccct taatattttt aaaaattatt
     9421 tctttataag aatccatact tcctttctct tttaaataaa cttgcttttc tagtattttt
     9481 tcatataata cttccatact ttcaaataat atagattctt tgttttcaac ttctaaaaat
     9541 tctaaagctt catcatttaa atagaataat ttctcaccct tagaaataaa tatataatct
     9601 ccaattgcag aattaatttt attaaacaac aattcttctc tatctaatag attttctaat
     9661 tccttcaact cttctatttt ttttcttaaa tacctattat ttctatcaaa ttcac
//
LOCUS       DAEVGI010000092         9663 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEVGI010000092 DAEVGI010000000
VERSION     DAEVGI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 9663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9663
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.092"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..204)
                     /locus_tag="K8M77_001770"
     CDS             complement(<1..204)
                     /locus_tag="K8M77_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7086262.1"
                     /translation="MSDIENDYMYLKLSKVKNLINIILVIATILYIVYFFRPLTAFYL
                     PIIIETILVFSITFIATTVFKFSN"
     gene            complement(314..1249)
                     /locus_tag="K8M77_001771"
     CDS             complement(314..1249)
                     /locus_tag="K8M77_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI7086263.1"
                     /translation="MDNNYRIIDNYIRSGDKAHDEGNLIKALNYYLRAEELLEGERDI
                     DLIISIALMLDELGDTEKAKEKYEEALSINSFEVRAYYGLAVIYDEKEDYVKAIELYK
                     KAIDINPVYDRAIFFLANALDNVGDKEGAIEYYKKTIEVCKEDFWAHINLGSIYEERD
                     MLQEAYRMFSKALQIDGEHYLALFNMGVIYKRLNVYDKAKKFYEKAIKKNFGYAFSFL
                     NLAVIYKEENNIEKGIEILSLGLRFNPENHFLYYNRSCFKAILGKDLEDATDDLIKAV
                     EFYPEFIKYINKDNDLIEVRNSNKFKDFLEAINIG"
     gene            complement(1372..2292)
                     /locus_tag="K8M77_001772"
     CDS             complement(1372..2292)
                     /locus_tag="K8M77_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HBI7086264.1"
                     /translation="MRENFIFKDDEGLELQGYKWSNSKEFKAVIHILHGMTEDAIRYD
                     EFAEKLVEAGFLVYAHDHRGHGFTAKDLESLGYQAENDGFQWMIDDTKTLIESSKEKH
                     KGYKIILFGHSMGSFVSQRLVQEYNDLVDVLVLSGTNGEPDKLAPLGEFVAQVEMSLK
                     GREHKSKLMDKLIFGGFNKNFKPNRTDFDWLCSVESEVDKYIENERYGFICSSSFYYD
                     LLRGVRSIHKAENMNRINKDMPIYIFGGDKDPVGNFGKGVINLKDKLEKVGVKNIEYK
                     LYENGRHEMLNEKNKVEVMEDTIQWLLKNI"
     gene            complement(2415..3011)
                     /locus_tag="K8M77_001773"
     CDS             complement(2415..3011)
                     /locus_tag="K8M77_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086265.1"
                     /translation="MSKNFDYSLKDISSNLEKSLKEAELLGEGHNGVVFLLENNKAIK
                     IFRRMSVWKDESSILKRVRKSRFFPRIYEAKPGYIIREYVDGVRLDKYLRRGNLDENL
                     CKELYLMILEFERLGFKRIDIRCKDIYIQDDYTLKIIDPKNNYKKRVSFPRHLFKGLF
                     KRDELNRFLNYVAEIDEKRAHIWREKFEIYAEDKKILI"
     gene            complement(3152..3237)
                     /locus_tag="K8M77_001774"
     tRNA            complement(3152..3237)
                     /locus_tag="K8M77_001774"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Leu,seq:gag)
     gene            complement(3297..4412)
                     /locus_tag="K8M77_001775"
     CDS             complement(3297..4412)
                     /locus_tag="K8M77_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HBI7086266.1"
                     /translation="MKKWLIAIGGILIIAIFILFFNREYLKPVHMDVNWNDNFNPPEE
                     KMLFDFIEKDLSKSGYGIYTNYIDKSSEGDITKGYSVLSESEGLMMLYSVNSNNKELF
                     DEHFDIVKEMRLKNGLISWRKEGDKNSPSSATIDELRIIKALLLASNRWNSFYYKFYA
                     INIANSLLKHAEENKTLVDYIDDYGKGNTTTLCYLDLPTMKLLSQVDKKWEGIYEKSN
                     GIIENGRISEEVPLYRKVFYEETQKYDEEENVDFLLSTIVILNRIEAGENEESSIKWI
                     KEKFKKDGFLVATYNGKNGDATSQIESPSIYSNVALIANYIGDKELFNKAIDKLKYYQ
                     IKNKDSVLYGGFGDEKTNSVYSFDNLNALLAFQKYKD"
     gene            complement(4464..5009)
                     /locus_tag="K8M77_001776"
     CDS             complement(4464..5009)
                     /locus_tag="K8M77_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBI7086267.1"
                     /translation="MKKIIRLVIIVLAIYLLFLGSGYILKNVIFPLKYMNYVEKYSKE
                     YNLDPYFVMAVMKAESNFDEDAKSNKDAKGLMQITTSTGKWIAEKQGIIDFDTNLLLE
                     PEVNIRFGCWYLNNLHKEFGDWDLVIAAYNAGRGRVQEWLNSNEHSNDGKNLTYIPFK
                     ETDKYVKKVNVYYNIYRSLYS"
     gene            complement(5019..5618)
                     /locus_tag="K8M77_001777"
     CDS             complement(5019..5618)
                     /locus_tag="K8M77_001777"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBI7086268.1"
                     /translation="MIKVGLTGGICSGKSTISSMIKEAGIPVIDADIIAREVLEKYPD
                     ILLRVKATFGGHFFDWRGDFRRREFGNHIFRFPKERIKYEEIIMPYIKEEIELKLKEY
                     EKINTKLVVVDGATLIENDMHKDMDMVVLVWVDKSSQIERMGFRDKLSKGEAINRINS
                     QLSLERKKDYANIIIDNSGNLIKTKEQIDDLLEFFTLYQ"
     gene            complement(5635..8235)
                     /gene="polA"
                     /locus_tag="K8M77_001778"
     CDS             complement(5635..8235)
                     /gene="polA"
                     /locus_tag="K8M77_001778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBI7086269.1"
                     /translation="MKRLLILDGNSLLNRAFYAVPLLSNSEGTHTNAIYGFTNMLFKM
                     REDIKPDYIVCTFDKKAPTFRHLEYGDYKAGRKKMPPELAEQFPIVKEMLKLFSITTY
                     ELDGFEADDIIGSLAKVAEGEGIEVYIITGDKDALQLAAKDINVCITKKGVSEIEVYN
                     YDRMVEEYGVTPTQFIDVKGLMGDKSDNIPGVPGVGEKTAFKLIKEYGSVEAVLENID
                     NISGKKLKENLTEYSEQAIFSKKLATIMTNVPIDFDLDEIKSKDDYNKDQLKALFTKL
                     QMKSILDKLSNGEEEEPEIEVKITNIENNKKLEDLFNNINEEVYINFTYLNKEKYSTI
                     EIDKIFINYDEKNYLINFKELAQEEENIKALKEFFENEDILKNGYSIKNALTALKKLG
                     IEFKGLAFDCELAAYLIDSSKGEYTLLSLINNYLSKFPNVKDGEEDLVGISFMKELTL
                     VLKKKIEEEKMERLMYDIELPLETVLSSMESIGFKVNKDILDELSIKFKRELEETQKE
                     IFELADEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEVLEKLKDKHPIIEKIT
                     YFRQLSKIYSTYVEGLKSVIDTDGSIHSTFNQTVTTTGRLSSTEPNLQNIPIKYEMGR
                     EIRKVFIPKEEGDLLISADYSQIELRVLAHISGDENMIDAFNHHSDIHTKTASEVFKV
                     PVEEVTPLMRSRAKAVNFGIVYGIGDFSLSQDLNISRKEAKEYMDIYFERYPKIKEYI
                     SKVIHEAQTKGYVLTMLNRRRYIPEANASNKIVKALGERLAMNAPIQGTAADIIKLAM
                     VNVYNKLKELNLNSKLILQVHDELIINVVKGELDIVKKLLKEEMENTIKLSVPLDVDV
                     NVGEDWYKLK"
     gene            complement(8655..9572)
                     /gene="glsA"
                     /locus_tag="K8M77_001779"
     CDS             complement(8655..9572)
                     /gene="glsA"
                     /locus_tag="K8M77_001779"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBI7086270.1"
                     /translation="MKNFLESLIENNRQYVEHGKLASYIPELEKANKEDLGIYIVTLD
                     GKEVGAGEYDKKFTIQSISKVITLMLAILDNGKDRVFSRVGVEPTGDSFNSIVSLERK
                     PSKKPFNPMVNSGAILTTSLIEGDSEEEKFERILEFTRKVTGNDDIQLNEDVYLSEKE
                     TGDRNRALAYFMKSNGVIEGNIDDILDLYFKQCSLEVNAKDLARFGAMLANDGVLPWN
                     GERVISREICRIIKTIMVTCGMYDDSGKFAVHIGIPAKSGVGGGIMAAVPRRMGIGIF
                     GPSLDDKGNSIAGTHVMKDLSEELDLSIF"
BASE COUNT         3137 a         1792 c          845 g         3889 t
ORIGIN      
        1 attagaaaat ttaaatacag ttgtagcaat aaatgttata ctaaacacca atatagtttc
       61 aattattatt ggtaaataaa atgctgtaag tggtctaaaa aaatatacaa tatacaaaat
      121 agttgctata actaaaataa tattaattaa atttttaact ttactaagtt ttaaatacat
      181 atagtcattc tctatatcac tcaatatact cctccccctt aaatatacat tatcataaaa
      241 aaacataata aattatttat tttttgtaaa ttattgtatt taaatgtaaa ttaaaaaaac
      301 ataaatactg gtattaacca atatttatgg cctctaaaaa atctttaaac ttattactat
      361 ttcttacctc aattaaatca ttatctttat ttatatattt tataaattct ggataaaatt
      421 caacagcctt aattaaatca tctgtagcat cctctaaatc ttttcctaaa atagccttaa
      481 agcaacttct attataatat aaaaaatgat tttctggatt aaatcttaaa cccagagata
      541 aaatctctat tcccttttct atattatttt cttccttata tataactgcc aaatttaaga
      601 atgaaaaagc atacccaaaa ttctttttaa tggctttttc ataaaatttc ttagccttat
      661 catatacatt taatctctta tatattactc ccatattaaa aagtgctaaa tagtgctctc
      721 catctatttg aagagcttta gaaaacattc tataagcctc ttgaagcata tccctttctt
      781 catatataga tcctaaattt atatgagccc aaaaatcttc tttacaaacc tctattgtct
      841 ttttgtaata ctctattgca ccttctttat ctcccacatt atctaatgca tttgctaaga
      901 aaaatatagc tctatcataa actgggttta tatctatagc ctttttatat aattctatag
      961 cctttacata atcctctttt tcatcataaa tcactgctaa tccataataa gctctaacct
     1021 caaaactatt tatacttaag gcttcttcat atttttcctt ggccttttca gtatctccta
     1081 attcatctag cattaaagct atacttatta ttaaatcaat atctctttct ccttctaaaa
     1141 gctcctcagc tcttaaataa taatttaagg cctttattaa attcccttca tcatgagctt
     1201 tatcaccact acgtatataa ttatctataa ttctatagtt attatccata tatcgtctcc
     1261 tatttttcta atctattata tcaaaattat cttaacttac ataaagcaaa aaggaggtat
     1321 caaaattttg acacactcct aattaatact ctaattaatt atatatatct tttaaatatt
     1381 tttcaacagc cactgaatag tatcttccat aacttctact ttattttttt catttaacat
     1441 ttcatgtctt ccattttcat atagcttata ctcaatattt tttactccta ctttttctaa
     1501 tttatctttt aagtttatta cacctttacc aaaattccct actggatctt tatctccacc
     1561 aaaaatataa ataggcatat ccttgtttat tctattcata ttttcagctt tatgtatgct
     1621 tcttacccct cttaacaaat cataataaaa ggaacttgaa catataaaac catatctttc
     1681 attttctata tacttgtcta cttcactttc tacactacaa agccaatcaa aatctgtcct
     1741 atttggttta aaattcttat taaagcctcc aaaaatcaac ttgtccataa gtttactttt
     1801 atgttctctt ccctttaatg acatttctac ttgagctaca aattctccaa gtggagctaa
     1861 cttatctggc tcaccattag ttcctgataa aactaacaca tccactaaat cattatattc
     1921 ttgaactagc ctctgactta caaaagatcc catactatgt ccaaaaagta ttattttata
     1981 tcctttatgt ttttctttag aagactctat taaagtttta gtatcatcaa tcatccattg
     2041 gaaaccatca ttttcagctt gatatcctaa agattctaaa tctttagctg taaaaccatg
     2101 ccctctatgg tcatgggcat aaactaaaaa tcctgcttca acaagttttt ctgcaaattc
     2161 atcatatcta atagcatcct ctgtcattcc atgtaatatg tgaataactg ccttaaattc
     2221 tttagaatta ctccatttat atccttgtaa ttctaatcct tcatcatctt taaatataaa
     2281 attctctctc aaatccatca actcctttta gactgtgtag tataaaaatc ttcttaatac
     2341 taagatacta cttaatctaa aatctttaaa cagatgcttt aagcgattta ctatttttta
     2401 tcaacttatt aatattatat taatattttt ttatcctctg cataaatttc aaatttttct
     2461 ctccatatat gagctctttt ttcatcaatt tcagctacat aatttaaaaa tctatttaac
     2521 tcatctcttt taaaaagacc cttaaaaaga tgtctaggaa atgaaaccct ctttttatag
     2581 ttatttttag gatctattat tttaagagta taatcatctt gtatatatat atccttacac
     2641 cttatatcaa ttcttttaaa tcccaatctt tcgaattcta aaatcattaa atataattcc
     2701 ttgcaaagat tctcatctag atttcccctt cttagatact tatctaatct aaccccatca
     2761 acatattctc ttataatata tcctggctta gcttcatata ttcttggaaa aaatctagac
     2821 tttctaactc gttttaatat actgctttca tccttccaaa cactcattct tctaaaaatt
     2881 tttattgctt tattattttc aaggagaaat acaacaccat tatgcccctc ccctaataat
     2941 tctgcttctt ttaaagattt ttctaaatta ctacttatat ctttaaggct ataatcaaaa
     3001 tttttactca ttttacccct cttattaata agtgcttaat actatattat acatttttta
     3061 taataaaaaa tgactaattt cttaattaag aaattagtca ccttgaataa gtatataaaa
     3121 atttaagtta tgctattctt taataccaaa ttggtgcaga tagcgggact tgaacccgca
     3181 cactcctaaa agtaataccc cctcaagata tcgtgtctgc cattccacca tatctgcata
     3241 aaatattgcc cattttaggt atatgaacaa tattatttat attattactt attattttaa
     3301 tccttatatt tttgaaaagc tagtagtgca tttaaattat caaaagaata tacgctattt
     3361 gttttttcat ctccaaatcc accataaagc acactatctt tattttttat ttgataatat
     3421 tttaatttat ctatagcctt gttaaataat tccttatctc caatgtaatt tgctattaaa
     3481 gctacattag agtatattga tggagattca atctgtgagg tagcatctcc atttttacca
     3541 ttgtatgtag ctactaagaa tccgtctttc ttaaactttt cttttatcca tttaatagat
     3601 gactcctcat tttctccagc ttcaattcta tttaaaatta ctattgtaga taataagaaa
     3661 tcaacatttt cttcttcatc atatttttga gtttcttcat aaaatacttt tctatataaa
     3721 ggaacctctt cagatattct tccattttct attataccgt tagatttttc ataaattcct
     3781 tcccacttct tatctacttg actcaataat ttcatagtag gcaagtctaa ataacataaa
     3841 gtagttgtat tcccttttcc atagtcatct atataatcta ctaaagtttt attttcttct
     3901 gcatgtttaa gtaaagagtt agcaatattt atagcataaa atttataata aaaactattc
     3961 catctgttgc tggctaaaag aagagctttt attattctaa gttcatctat agttgcagag
     4021 gacggtgaat ttttatctcc ttctttcctc caactaataa gtccattttt taatctcatt
     4081 tcttttacta tgtcaaaatg ctcatcaaat aattctttat tattagaatt taccgaatat
     4141 aacatcataa gcccttctga ctcagataat actgagtacc ccttagttat atctccttct
     4201 gaacttttat ctatataatt tgtatatatt ccatatccac ttttacttaa atccttctct
     4261 ataaaatcaa aaagcatttt ttcttctggt ggattgaaat tatcattcca attaacatcc
     4321 atatgtactg gttttaaata ctctctatta aaaaataata taaaaatagc aattattaaa
     4381 ataccaccta tagctattag ccattttttc aaaacatcac ctcccaaagc tttatccata
     4441 gcgtattaaa atatataggt cacttaagaa taaagtgacc tatatatatt gtaataaaca
     4501 tttacttttt taacatattt atcagtttct ttaaatggta tataagtaag attttttcca
     4561 tcgttagaat gttcattact attaagccat tcttgaaccc gtccccttcc tgcattgtaa
     4621 gctgcaatta caaggtccca atctccaaac tctttatgaa gattatttaa ataccaacat
     4681 ccaaacctta tattaacttc tggctctaat aataaattgg tatcaaaatc aataatccct
     4741 tgtttttcag ctatccactt tccagtagag gtggtaattt gcataagccc ttttgcatcc
     4801 ttattagatt tagcatcttc gtcaaagtta ctttctgctt tcataaccgc cataacaaaa
     4861 taaggatcca aattatactc tttactatat ttctctacat aattcatata ttttagtgga
     4921 aatataacat tttttaatat atatccactg cctaaaaata gtaaatatat ggctaaaaca
     4981 attataacta gccttataat tttcttcata taactctcct actggtataa agtaaaaaat
     5041 tctaacaaat catcaatttg ttcctttgtt ttaattaaat taccgctatt atcaattatt
     5101 atgttagcat aatctttttt tctttcaaga cttagttgag aatttattct atttatagct
     5161 tcccctttac taagcttatc tctaaatccc attctttcta tttgactact tttatctacc
     5221 caaactaaaa caaccatatc catatcctta tgcatatcat tttctattaa ggttgcccca
     5281 tctacaacta caagcttagt gtttattttt tcatattctt taagtttaag ctctatttct
     5341 tcttttatat aaggcattat gatttcctca tactttattc tttcttttgg aaatctaaaa
     5401 atatggtttc caaactctct tcttctaaaa tcacctctcc aatcaaagaa atgacctcca
     5461 aaagtagctt ttacccttaa aagtatatct gggtattttt ctaatacttc tcttgcaata
     5521 atatctgcat ctataactgg gattcccgcc tcttttatca tagaagaaat agtgcttttg
     5581 cctgagcata ttcctcctgt aaggcctact ttaatcatag aatcagtacc ctccttattt
     5641 taacttatac caatcttctc caacattaac atcaacatct aatggtacac ttaatttaat
     5701 tgtattttcc atttcctctt ttagaagttt cttaacaatg tctaattctc ctttgacaac
     5761 atttattata agttcatcat gaacttgtaa tattaactta ctatttaaat ttaattcttt
     5821 taacttgtta tatacattta ccatagcaag ctttattata tctgctgctg ttccttgtat
     5881 tggagcattc attgcaagtc tttctcctag ggcttttact attttatttg aagcatttgc
     5941 ttcaggtata tatcttcttc tatttaacat agttaataca tatccctttg tttgagcttc
     6001 atgaataacc ttagaaatat actctttaat ttttggatat ctttcaaaat atatatccat
     6061 atattcttta gcttcttttc ttgaaatatt taaatcctgt gaaagactaa aatctccgat
     6121 tccatatact ataccaaagt ttactgcctt agccctactt ctcattaatg gagttacctc
     6181 ttcaactggt actttaaaca cttcagaagc agtcttagtg tgtatatctg aatgatgatt
     6241 aaatgcatct atcatatttt catcaccaga aatatgagct aaaactctaa gttctatttg
     6301 tgagtagtca gctgatatta ataaatctcc ttcttctttt ggtataaata cctttcttat
     6361 ttctctaccc atttcatatt ttataggtat attttgaaga ttaggctctg ttgatgaaag
     6421 tctacctgta gtagttacag tttgattaaa tgttgaatgt atagaaccat ctgtatctat
     6481 aacagacttt aatccttcaa cataagttga atatattttt gaaagctgtc taaaatatgt
     6541 tattttttct attataggat gtttgtcctt aagcttttct aatacttctg cgttagttga
     6601 atatccagtt ttagttttct ttataactgg taaatctaat ttttcaaata atatcttacc
     6661 taattgtttt ggagaattta tattaaactc ttcatctgca agttcaaaga tctctttttg
     6721 tgtttcctct aactctcttt taaacttaat agataactca tctaaaatat ctttatttac
     6781 tttaaatcct attgattcca tagaagataa aactgtttct aaaggaagct caatatcata
     6841 cataagtctt tccattttct cttcttctat ctttttcttt agaactaaag tcaactcttt
     6901 cataaatgaa attccaacta agtcttcctc tccatctttt acattaggga attttgaaag
     6961 atagttattt attaaggata aaagagtata ttctccctta gatgaatcta ttaaatatgc
     7021 tgctaattca caatcaaagg ctaatccctt aaattctatc cctaattttt ttaatgctgt
     7081 taaggcattt tttatactat agccattttt taatatatct tcattctcaa aaaattcttt
     7141 aagggcttta atattctcct cttcttgtgc taactcttta aagttaatta aataattttt
     7201 ctcatcataa ttaataaata ttttatcaat ctctatagta gaatactttt ctttatttaa
     7261 ataagtaaaa ttaatatata cttcttcgtt tatattatta aataaatctt ctaatttttt
     7321 attattttct atattagtta ttttaacttc tatttctggc tcttcttctt ccccatttga
     7381 taatttatct aatatagatt tcatttgaag ctttgtaaat aaagctttta actgatcttt
     7441 attataatca tcctttgatt taatctcatc taaatcaaaa tctataggaa cattagtcat
     7501 aatagtagct aactttttag agaatatagc ctgttcgctg tactcagtta aattttcttt
     7561 taacttttta ccacttatgt tatctatatt ttctaaaact gcctctactg aaccatactc
     7621 ttttattaat ttaaaggctg ttttttctcc tactcctggt acccctggaa tattatcaga
     7681 tttatctccc ataagtcctt taacatctat gaactgagta ggagtaactc catattcttc
     7741 aaccattcta tcatagttat aaacttctat ttcagaaaca cccttcttag ttatacatac
     7801 gtttatatcc ttagctgcta gttgaagagc atccttatct ccagtaataa tataaacttc
     7861 gattccttca ccttcagcca ccttagctaa acttcctata atatcatctg cttcgaatcc
     7921 atctaattcg taagttgtta ttgaaaataa ctttagcatt tccttaacaa taggaaactg
     7981 ctctgctaac tcaggaggca ttttctttct tccagcctta taatccccat actctaaatg
     8041 tctaaaagtt ggtgcttttt tatcaaaagt acaaactata taatcaggtt ttatatcctc
     8101 tctcatctta aaaagcatat ttgtaaaacc ataaatagca tttgtatgag ttccttcact
     8161 attgctaagc aaaggcacag cataaaaggc tctatttaaa agactgttcc cgtctaatat
     8221 caataatctt ttcatcctaa tcctccaata tattattcta ttaatctaac aaaaaacttt
     8281 actattaata aatctcactt ctaatctact ctgtaaaaag tagttaaaat aaaattataa
     8341 ataatatgaa gagaatatct agatgaattt tagaatttaa gctattacgt ataataattt
     8401 gcgttaataa gcgaaatctg tttgaccgaa gggagttttt tcgctttagc aaattattag
     8461 aagtaattgc tataaataat gactacaatg taactaaagt tccattgtat aagttcaaga
     8521 accaaattaa aatttggact ttcacttaaa atctagtaat tctcgtaata tatttataat
     8581 tttattttta aagtttttaa acaatcatct cttttaatga tttttcccat tagttctaat
     8641 aatattaaca cattttagaa aatacttaaa tctaattcct cagataaatc cttcataaca
     8701 tgtgttcctg ctatactatt tcccttatca tctaaagaag gtccaaaaat ccctattccc
     8761 attcttcttg gaacagcagc cattattccg ccaccaactc cactctttgc cggaattcct
     8821 atatgtacag caaatttacc tgagtcatca tacataccgc aagtaaccat tattgttttt
     8881 attattctgc aaatttctct agatattact ctttctccat tccaaggtaa tactccatca
     8941 ttagcaagca tagcaccaaa tctagctaaa tctttagcat taacttctag agaacattgt
     9001 ttaaaatata aatctaatat gtcatctata tttccttcta taactccatt gcttttcata
     9061 aagtatgcta aagctctatt tctatctcca gtttcctttt cagataaata aacatcttca
     9121 tttaattgaa tatcatcatt tcctgttact tttctagtaa attctaaaat tctctcaaac
     9181 ttttcctctt cactatcacc ttcaattaaa gatgttgtca atattgctcc tgaattaacc
     9241 atagggttaa aaggtttttt agaaggcttt ctctctaaac ttactatact attaaagcta
     9301 tctccagttg gttcaacacc tacccttgaa aaaactctat cttttccatt atctaatatt
     9361 gcaagcatta aagttattac ttttgatata ctctgaatag taaacttttt atcatactct
     9421 cctgccccta cttcttttcc atcaagagta actatatata tacctaaatc ttctttatta
     9481 gctttttcta attctggaat ataagatgcc agttttccat gttcaacata ttgtctatta
     9541 ttttctatta aactctcaag aaaatttttc accttttttc gctccttagt ttaatttttt
     9601 tattcaataa attaataatt aatatataaa cattataaca ttttcaacga attattataa
     9661 atc
//
LOCUS       DAEVGI010000093         9480 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEVGI010000093 DAEVGI010000000
VERSION     DAEVGI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9480
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.093"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            49..1560
                     /locus_tag="K8M77_001780"
     CDS             49..1560
                     /locus_tag="K8M77_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7086271.1"
                     /translation="MKRIAIYSRKSVFIEGSISIETQINMCKEYMINKFPDATFEIFE
                     DEGFSGGNTNRPAFQKMLKLAELKEIDIVVCYKIDRIARNTLDFLNILEIFKKNNVEL
                     VSITEGFDPNTQMGKVMLTLLASFAEMERTNIQQRVKDSMFSLAQKGNWTGGAPPLGF
                     KVKESGGIEIYNKNLILDVFNMKFKKYKNTEIIEYIESKYNHKFVNTTLASMLRKPIY
                     TKSSKEVSTYLKTKGYKILREENNSNSYYTYKNNNQDYAIVNNDVDGLIEPYVWISIN
                     KAMDDNSNGETNRFSEKFWLTKSLRCKYCGKTYCGQTKNVKSKYTNKDGVSKEYIGTY
                     EYYMCRDMLRGRLKTCENTKRIKRSYLEKKIEELIYILKDKSFFEKSYYKREVDNPAK
                     ELKELESNMKKLDKTINNLTDKIALLSNDAAMILIDKIETLVKEKKALKDKMISLELK
                     SLDNLEGKEEFIYENILEFNTNLSVDQKRNIAINIFKEIVYDYKTDKFEVFFN"
     gene            complement(1569..2738)
                     /locus_tag="K8M77_001781"
     CDS             complement(1569..2738)
                     /locus_tag="K8M77_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBI7086272.1"
                     /translation="MNVTVTALGAVIALVIAITLIIKKVHPAYGLILGALIGGLVGGA
                     GLTGTVNLMMDGAKNMMPAIFRILTAGVLAGVLIESGAAAKIAETIVEKIGESRALIA
                     LAIATMVLTMVGVFVDVSVITVSPIALAIAHKSGLSKPAILLAMVGGGKAGNIMSPNP
                     NTIAAADNLGVSLTNVMIAGIVPAIFGVIITCILASRIKQKGSLVESDEHMKELTDMP
                     GFLPAMVGPLVAIILLMLRPIAGIAIDPLIALPVGGVCGILAMGKIKHINKYATYGLA
                     KMSGVAILLIGTGTLAGIIANSGLKDVIISGINALGLPSFMLAPVSGILMSAATASTT
                     SGTAVATSVFGPTIVQLGVAPLATAAMIHAGATVLDHLPHGSFFHATGGSAILLS"
     gene            complement(2886..3947)
                     /locus_tag="K8M77_001782"
     CDS             complement(2886..3947)
                     /locus_tag="K8M77_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086273.1"
                     /translation="MLNRELAQNIVNKMMEVIPYNVNIMNHKGVIIGSGDSSRIGMIH
                     NGALEALRVKKVVEISKDGDKVKSGVNSPIFFREKAIGVIGITGNPKNVRQFTQLVTV
                     TAELLINQEYTLNKHIVKERLKEEFIYEWLYSKEAYDLEFIQRGANVGIDISLDRIIL
                     VLEFQKDQFKEIIKKIKRFIKEDEYYINLTSNRIVVILLDNNLLFKRLKELKEIMSDT
                     NSKIGLSSKHKIISKSMNEAVEALDIGNKLYKNEFFYDYKKVKVFSYLDNFLDEEEWK
                     IIKNKILEENMGEELLETFISYMKHSGERKKTAESLHIHRNTLNYRLERIEEITGLSF
                     DNYLDLFQILIAYIVSQLK"
     gene            complement(4206..4592)
                     /locus_tag="K8M77_001783"
     CDS             complement(4206..4592)
                     /locus_tag="K8M77_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086274.1"
                     /translation="MNHKKRSKILLVSGIIGTIYSLLLIRFFLAYFSVGIISDIDIEK
                     AVSALAVIIVIPHMVLFVLATIFNWFSYIKNKRGFALSAGILYLISLIVFPMDLIFVV
                     PSTILSFIGYFRLKKINNSKQENNCM"
     gene            complement(4691..5863)
                     /locus_tag="K8M77_001784"
     CDS             complement(4691..5863)
                     /locus_tag="K8M77_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBI7086275.1"
                     /translation="MNNFTFYNPTKINFGRGVESSVGEEIKSLGASKVLFHYGGGSIR
                     KNGLYDRILDSLNKAGLHVIELGGVKPNPRLSLVKKGIELCKNSKVDFILAVGGGSVI
                     DSAKAIALGVPYDGDVWDFFTKNIKIEKALPLGTVLTIPAAGSESSSGTVITNEDGWY
                     KRSTGSPLLYPKFSMLNPELCFTLPEYQIASGSADILVHLMERYFTNTKNVELIDRLI
                     EGTMKTVINNVPKVLKNKEDYDSFAEVMWAGTIAHNNLLSTGRETDWASHNIEHELSG
                     IYDVTHGAGLAVIFPAWMKFVYKHDLDRFNQFATRVFDVKVEGKTKGEIALEGIKKLE
                     EFFKSINLPVTLKELEIGEDRLEEMAKKCTDNDEHTVGHFVELNAEDILEIYKLAL"
     gene            complement(6190..9258)
                     /locus_tag="K8M77_001785"
     CDS             complement(6190..9258)
                     /locus_tag="K8M77_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBI7086276.1"
                     /translation="MLFQINERKNIVNGLCEKLEKAIYNVVAPLEIDMYITKEPVSYE
                     NRLTGEHKKGVIGESWGELWDCGWFNFKGEVPKSYEGENIVLLIDISGEAFVVDKDGN
                     PMRGLTTLNSEFDLSLGMPGKREVPMFERAKGGEVIDIWADCACNDLFGKYRDNGIIK
                     DAYIATCNEEVKALYYDVEVLHELMNQLPEDSARYNTILNALYEASKVLSIKTGMSGC
                     DFLKDGIVTQQEVTILNEEEVKKAREILKKELDKKGGDPSLSVSAIGHAHIDLAWLWP
                     IRETIRKGARTYSTVLANMEKYPEYVFGASQPQLYQWMKEYYPDLYERIKEKIEEGRW
                     EAQGGMWVEPDTNVPSGESLVRQLLYGKRYFREEFKKEMDTLWLPDVFGYSAALPQIL
                     KKSGVDYFMTIKLSWNNHNQFPHHTFVWEGLDGSRVLSHMPPEGTYNSSAAPRAVAKS
                     EKAFLDKGLSDECLMLFGIGDGGGGPGEEHLERLRRERNINGIAPVKQEPSSNFFKRI
                     EKDIDKYSVWSGELYLEKHQGTYTTHGKNKKYNRKMEIALKNLELAAMQAKVLGKGEY
                     PQEEIEKVWKEVLLYQFHDILPGSSIGRVYDESVEAYEKMLAKVEGMTEKLYRDILES
                     AYSKEEGAILVNSLSWSREKWIKLYDRWTRVNLNSLSGEIIKKEEIEPYCEISTLKVS
                     ETNLENGLVKVEFNENGTIARIFDKELNKEVLRDEKGNELRVYEDNGDAWDFSGVYED
                     RPSEIFDLVKSEVIVDGPKATIKQNYKYGDSTLVQEISILEGSKRINFKTKVHWKENG
                     KMLRTSFNTNVYTREADCEIQFGTIKRPTHGNTSWDMAKGEICAQRWIDLSQRDYGVA
                     LINDSKYGHNVSETKIDLNLLRSPGYPDPNADRGEHEFTYCLFPHRGDYIEGNVVHEA
                     HEVNAPIQVIYSENLGENLVKEAMASIDCENVIIDTIKKAEDGDQMIIRLYECHGEDA
                     KAKVNINVPYEKVEMVNLIEDSIDGKELNKETMHLTFKPFEVHTLKVTLK"
BASE COUNT         3114 a         1729 c         1074 g         3563 t
ORIGIN      
        1 taaataaaaa tctcataata ttcaacttaa tacttaagga ggtataatat gaaaagaatt
       61 gctatttatt caagaaaatc tgtttttatt gaaggttcta tatcaataga aacgcaaatt
      121 aatatgtgta aggaatatat gataaacaaa tttcctgatg ctacttttga aatttttgaa
      181 gatgaaggat ttagtggtgg caatactaat agacctgcat ttcaaaaaat gcttaaatta
      241 gctgaattaa aagaaataga tatagttgtt tgttataaaa tagatagaat agcaagaaac
      301 actttggact ttctaaacat attagaaata ttcaaaaaaa ataatgttga acttgtatca
      361 ataacagaag gttttgatcc aaatacacaa atgggaaaag ttatgctaac tttattggct
      421 agttttgctg agatggaaag aactaatata caacaaagag ttaaggatag tatgttctct
      481 ttggctcaaa aaggtaattg gactggcgga gctcctccat taggatttaa agttaaagaa
      541 agtggtggca ttgaaatata caataaaaac ttaatattag atgtattcaa catgaaattt
      601 aaaaaatata aaaatactga aattatagaa tatatagaat ctaagtataa tcataagttt
      661 gtaaatacta ctttggctag tatgcttaga aaacctattt atactaaaag ctctaaagaa
      721 gtttctactt acttaaaaac aaaaggatat aaaatattaa gagaagaaaa taactcaaat
      781 tcatattata cttacaaaaa taacaatcaa gattatgcta tagtaaataa tgatgtagat
      841 ggtttaatag agccatatgt ttggatttca ataaataaag ccatggatga taattccaac
      901 ggtgaaacta atagatttag tgagaaattt tggcttacta aatcacttag atgtaaatat
      961 tgtggcaaaa cttattgtgg acaaactaaa aatgttaaga gtaaatatac aaataaagat
     1021 ggagttagta aagaatatat aggtacttat gagtattata tgtgtagaga tatgcttaga
     1081 ggtagattaa aaacatgcga aaatactaaa agaataaaaa gatcatattt agaaaagaaa
     1141 attgaagaat taatttatat tcttaaagat aagtctttct ttgaaaaatc atattataaa
     1201 agagaagttg ataatcctgc taaagaatta aaagaacttg aatcaaacat gaaaaagcta
     1261 gataagacta taaacaattt aacagataaa atcgctttac ttagtaatga tgcagctatg
     1321 attttaattg ataaaattga aactttagtt aaagaaaaaa aagctctaaa agataagatg
     1381 atttcattag aattaaaatc cttagataac ttggaaggaa aagaagaatt catttatgaa
     1441 aatattcttg aatttaatac taatttatct gtagatcaaa aaagaaacat agctattaat
     1501 atatttaaag aaatagtata tgattataaa acagataaat tcgaagtatt tttcaattaa
     1561 ttttttgttt aacttaaaag tattgcactt ccaccagtag cgtggaagaa gcttccatga
     1621 ggtaagtgat ctaaaactgt agccccagca tgtatcatag ctgctgtagc aagtggtgcc
     1681 actcctaatt gaacaattgt tggtccaaag actgatgtag ccaccgctgt tcctgaagta
     1741 gtagaggcag ttgccgctga cataagtatt cctgaaactg gagctaacat aaagcttggt
     1801 aatcctaaag cgtttattcc acttataata acatccttta atcctgagtt agcaattatt
     1861 cccgctaaag tacctgtacc tattaaaagt atagctactc cactcatctt agctagtcca
     1921 tatgttgcat atttgttaat atgcttaatt tttcccatag ctagtattcc gcatactcca
     1981 cctactggta aagctattaa tggatctata gcaatgccag ctattggtct taacattaat
     2041 aagattattg ctactaatgg tccaaccatg gctggtaaaa atcctggcat atctgttaac
     2101 tctttcatat gctcatcaga ttctactaaa gaaccttttt gttttattct acttgctaat
     2161 atacatgtaa ttataactcc aaatatagct ggtactatac ctgctatcat aacattagtt
     2221 aatgaaactc ctaaattatc tgctgccgca atggtatttg gatttggaga cattatattt
     2281 cctgccttac ctccaccaac catggctaat aatatagctg gtttacttaa acctgatttg
     2341 tgagctattg ctaaagctat tggagatact gttattactg aaacgtctac aaaaactcca
     2401 accattgtta aaaccatggt agctattgct aaggctataa gagctcttga ttctccaatc
     2461 ttctcaacta ttgtctctgc aatcttcgct gccgctcctg attcaattaa taccccagct
     2521 agtacccctg ctgtaagtat tctaaagata gcaggcatca tatttttggc tccatccatc
     2581 attaaattaa ctgtccctgt tagtcctgct ccaccaacaa gtccacctat taaagctcct
     2641 aatataagtc cgtatgctgg atgaaccttt tttatgataa gagtgattgc aataactaat
     2701 gcaattactg cacctaaagc tgtaactgtt acattcacaa taaattcctc cttcttatta
     2761 aactcattta ttcttattta tattgacgat tttcattgta taagttttca tattcaaaat
     2821 cattatttaa tatcactaaa ataaaaaggt tttctttatt caaacgcaca aagaaaacct
     2881 tttttttatt ttaattgact tactatatat gcaatcaaaa tttgaaataa atctaaataa
     2941 ttatcaaagc ttaagcctgt tatttcctca attctctcta gtctataatt taatgtattc
     3001 ctatgtatat gtaaggattc tgcggtcttt tttctctctc cactatgctt catataggat
     3061 ataaaggttt ctaataattc ttctcccata ttttcctcta atatcttgtt tttaataatt
     3121 ttccactcct cttcatctaa aaaattatct aagtaactaa aaacctttac ttttttataa
     3181 tcataaaaga actcattttt atataattta tttcctatgt ctaatgcttc cactgcctca
     3241 ttcatagatt ttgatataat tttatgtttt gagcttaatc ctatcttaga atttgtgtca
     3301 ctcataattt cctttaattc ttttaacctc ttaaagagca aattattatc taacaaaatt
     3361 acaactattc tatttgaagt taaatttata tagtactcat cttcttttat gaaccttttt
     3421 atttttttaa ttatttcctt aaattgatct ttctgaaact ccaatacaag tattattcta
     3481 tctaaggata tatcaatacc tacattggct cccctttgaa tgaattctaa gtcataggcc
     3541 tcttttgaat acagccattc ataaataaat tcctctttca atctttcttt aactatatgt
     3601 ttattcaaag tgtattcttg atttattaaa agttcagcag taacagttac aagctgtgta
     3661 aattgcctaa catttttagg atttcctgtt ataccaataa cccctatagc tttttctcta
     3721 aaaaatatag gtgaattaac tccagatttc accttatccc catccttaga tatttcaacc
     3781 acctttttta ccctcaaggc ttctaaagcc ccattatgta tcattcctat tctactcgaa
     3841 tcaccactac ctattattac tcccttatga ttcattatgt ttacattgta aggaataacc
     3901 tccatcattt tattaactat attttgagcc agttccctat ttaacattat attgcgctaa
     3961 gtatatcccc tgcttaaact tagctcctcc tctctttgaa tctcccataa ttacaccttg
     4021 gaatattcta tataagctta gataaactga tactagcaaa atatttaaac atttacaata
     4081 cttaaaataa aaaaggatat gtatcaaaac taaataatga aacttataaa atatataatc
     4141 tattaattct cttaatatac ttataagttt tattaaaaaa ttattttgat acataccata
     4201 ctattttaca tacagttatt ttcctgcttg ctattattta ttttcttgag tctaaaatat
     4261 cctataaagc ttaaaatcgt acttggaaca acaaaaatta aatccattgg aaatacaata
     4321 agtgatatta aataaagtat tcctgcactt aaagcaaatc ctcttttatt ttttatataa
     4381 gaaaaccaat taaatattgt agctaaaaca aataaaacca tatgtggtat aacaataata
     4441 accgctagag ctgatacagc cttttctata tcaatatcgc ttataatccc cacagaaaaa
     4501 tatgctaaaa aaaatctaat caaaagtaat gaataaatag ttcctattat tccagatacc
     4561 aataatattt ttgaacgctt tttatgattc attttgctaa aaactccctt aatttatact
     4621 attaaataag gctctacctt aaagttttat ttttattatt ttataaaaat ctatataaag
     4681 tattagaatt ttataaagct aatttgtata tttcaaggat gtcctctgcg tttaattcta
     4741 caaaatgacc tactgtatgc tcatcattat ctgtgcattt tttagccatt tcttctaatc
     4801 tatcttcacc tatttctaac tcttttaaag ttactggaag atttattgat ttaaagaatt
     4861 cttcaagttt cttaattcct tctaaggcaa tctctccctt agttttgccc tctactttaa
     4921 catcaaatac tctagtagca aattgattga atctatctaa atcatgctta taaacaaact
     4981 tcatccaagc tgggaatata acagctaatc cagcaccatg agtaacatca tatattccac
     5041 ttagttcatg ttctatatta tgtgatgccc aatctgtttc tcttcctgtg cttaaaagat
     5101 tattatgtgc tattgttcca gcccacataa cctctgcaaa gctatcatag tcttccttat
     5161 tttttaatac cttaggaaca ttatttatta ctgttttcat tgttccctca attaatctat
     5221 ctataagttc aacattcttt gtatttgtga aatatctttc cattaaatgt actaaaatat
     5281 ctgcacttcc tgatgctatt tgatattctg gtaaagtaaa gcatagttct ggatttaaca
     5341 ttgaaaattt aggatataga agtggtgatc ctgttgatct cttataccat ccatcttcat
     5401 tagttataac agttcctgag cttgattcac ttcctgccgc tggtattgtt aatactgtac
     5461 ctaatggtaa agctttttct attttaatat ttttagtaaa gaaatcccat acatctccat
     5521 cataaggaac accaagagct atagctttag ctgagtcaat aacacttcct cctccaactg
     5581 caagtataaa gtcaacttta ctatttttac aaagctctat tcccttttta actaaactta
     5641 atctaggatt aggcttaact cctccaagtt ctattacatg taatcctgct ttatttaaag
     5701 agtctaaaat tctatcatat aacccatttt ttctaatact tcctccacca taatgaaaaa
     5761 gcactttact agctcctaag cttttaatct cttctccaac agaggattca acccctctac
     5821 caaagttaat tttagtagga ttataaaaag taaaattatt catttaactc accctttctc
     5881 ttttttccat attatttaaa ttaattacgt ttacaatatt atatatcact ttttatttta
     5941 agtaaaagat tatataaata tattttacaa acaaaaaaga caaccctcct caaaggttgc
     6001 cttttcctta tacttaattt ccccccttgg caagctatgc ttaccatttt ctttaagtaa
     6061 ttagaagtgt ttcttttaat tcccttattg tctgttagta ttatcttcta tataaatatt
     6121 acttaaattc ttaattataa tttaaagctt aaagttcttt aaatgtttta atactcttta
     6181 aattcttaac tactttaatg ttacttttaa agtatgaacc tcaaatggtt taaatgtaag
     6241 gtgcattgtt tctttgttta attcttttcc atctatgcta tcttctatta agttaaccat
     6301 ttctaccttt tcatatggaa catttatatt aactttagcc ttagcatctt ctccatgaca
     6361 ctcatataat ctgattatca tttggtcacc atcttcagct tttttaattg tatcaattat
     6421 tacattttca caatctattg aagccattgc ttctttaact aggttttcac ctaagttttc
     6481 actataaatt acttgtattg gagcattaac ttcatgagct tcatgaacaa catttccttc
     6541 aatatagtct cctctgtgtg ggaataaaca ataagtaaat tcatgctctc ctctatcagc
     6601 atttggatct ggatatcctg gacttcttaa tagatttaag tctattttag tttctgaaac
     6661 attgtgtcca tacttagaat catttattaa agcaactcca taatcccttt gtgataaatc
     6721 tatccatctt tgagcacata tttcaccctt agccatatcc catgaagtat ttccatgagt
     6781 aggtctcttt attgtaccaa attgtatttc acaatctgcc tctctagtat aaacattagt
     6841 attgaaagat gttctaagca tcttaccatt ttctttccaa tgaacctttg ttttgaagtt
     6901 gattctcttg ctaccttcta aaatgcttat ttcttgtact aaagtagaat ctccatattt
     6961 atagttttgc ttaatagttg cctttggtcc atctacaata acttctgatt ttactaaatc
     7021 aaaaatttca gatggtctat cttcataaac tcctgagaaa tcccaagcat ctccattatc
     7081 ttcataaact ctaagttcat ttcctttttc atctcttaag acttctttat ttaattcttt
     7141 atcaaaaatt cttgcaattg ttccattttc attaaactca actttaacta atccattttc
     7201 taagttagtt tccgaaactt ttaaagttga aatttcacaa tatggctcaa tttcttcttt
     7261 tttaattatt tctccactta aagagtttaa gttaactctt gtccatctat cataaagctt
     7321 aatccacttt tctctactcc atgataaaga gtttactaat atagctcctt cttccttact
     7381 atatgcactt tctaaaatat ctctatataa cttttcagtc attccttcta ccttagcaag
     7441 catcttttca taggcttcca ctgactcatc ataaactctg cctatggatg atcctggtaa
     7501 aatatcatgg aattgatata aaagaacttc cttccaaact ttttctattt cctcttgagg
     7561 atattctcct tttcctaaaa ctttagcttg cattgctgct aactctaagt tcttaagagc
     7621 tatttccatc tttctattat attttttatt cttaccatgt gtagtgtacg ttccttggtg
     7681 tttctctaag tataactccc cactccacac actgtattta tctatatcct tttctattct
     7741 cttaaagaaa ttacttgaag gttcttgctt aacaggagct attccattta tatttctttc
     7801 tcttcttaat ctctctaaat gctcttctcc tggtccaccg cctccatcac caattccaaa
     7861 tagcattaaa cattcatctg ataagccttt atctaagaaa gctttctctg atttagcaac
     7921 agcccttggt gcagctgaac tattataagt tccctctggt ggcatgtgtg ataatactct
     7981 acttccatct aatccttccc aaacaaaggt atgatgtgga aattggttat gattgttcca
     8041 tgataattta attgtcatga aataatctac accactcttt ttaagaattt gtggaagcgc
     8101 tgctgaatat ccaaatacat ctggaagcca taaagtatcc atctcttttt tgaattcttc
     8161 tctaaagtat ctctttccat ataataattg tcttactaaa gattctcctg atggaacatt
     8221 agtatctggt tcaacccaca tacctccttg agcttcccat cttccttctt ctattttttc
     8281 tttaattctt tcatataaat ctggatagta ttctttcatc cactgataaa gttgaggttg
     8341 gcttgctcca aatacatact caggatattt ttccatatta gctaaaactg ttgaatatgt
     8401 tctagctccc tttcttatag tttcccttat tggccataac caagctaaat ctatatgagc
     8461 atgtccaata gctgaaactg aaagtgaagg atctccacct tttttatcca attctttctt
     8521 taagatttct ctggcttttt taacttcttc ttcatttaag attgtaactt cttgttgagt
     8581 aacaattccg tcctttaaga aatcacatcc actcattcca gttttaatac ttaatacctt
     8641 tgatgcttca taaagagcat ttaatatagt attatatcta gcactatctt ctggtaattg
     8701 attcataagt tcatgtaaaa cctctacatc ataataaaga gcctttactt cttcattaca
     8761 agttgcaata tatgcatctt ttataattcc attatctcta tatttaccaa acaaatcgtt
     8821 acatgcacaa tctgcccata tatctataac ttctccacct ttggctctct caaacatagg
     8881 aacctctctc ttacctggca ttcctaagct taaatcaaac tctgagttta aagttgtaag
     8941 ccctctcata ggatttccat ccttatctac cacaaaggct tctccactta tatctattaa
     9001 aaggacaata ttttctcctt cataactttt aggtacttct cccttaaagt taaaccaacc
     9061 acagtcccaa agttctcccc aagactctcc tataactcct tttttatgct ctccagttag
     9121 tctattttca taacttactg gctccttagt tatatacata tctatttcta aaggcgcaac
     9181 cacattgtat attgcctttt ctagcttttc acacaaaccg tttacaatat tcttgcgttc
     9241 atttatctga aataacattc atctcacccc ttgataaatt taaaaatcat aaaataaatc
     9301 tttagaatcc taccttaata attaaattaa tgggcttctt ttctttcgat tcatctaaat
     9361 tctctatttt aaagatatct tataagcaat taagtgtaaa gtggacaaat attagtttta
     9421 tagttcatat ttttaataat tgaaattcaa tttaaaatct cttcaattat aattttaact
//
LOCUS       DAEVGI010000094         9333 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEVGI010000094 DAEVGI010000000
VERSION     DAEVGI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.094"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(129..851)
                     /locus_tag="K8M77_001786"
     CDS             complement(129..851)
                     /locus_tag="K8M77_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI7086277.1"
                     /translation="MRRESNPALAAGFEKAVSDGTTMTVGGTIVKILIMTAILGVAFV
                     YSWIAFQNPDVNYKSALVVSLGGSLILGLLTSFIPKIAQFTAVFYAAFEGVLLGSISR
                     YFESMFPGIVFPAMLLTIICLVATVLIYRRTPDIAGRISRGVFIAIISIGAIYLLGMV
                     LSFFGITLPIYGSGIIGIGFSLFVVAIATASLIMDYDFILKASQYGYPKYMEWYGAFG
                     LMVTLIWLYTEILNLLAKFSDN"
     gene            complement(1025..1363)
                     /locus_tag="K8M77_001787"
     CDS             complement(1025..1363)
                     /locus_tag="K8M77_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086278.1"
                     /translation="MKFKKNKLTLLIFTLIVISTAIIFYILKPINLFKFILFLIYVFL
                     EILLFRLWILSTYGFICIDCGNTFNIGILKRLLFFKKNKECPKCKGRNIVKEHLWSYC
                     LHSWNDLDED"
     gene            complement(1399..2793)
                     /gene="gltA"
                     /locus_tag="K8M77_001788"
     CDS             complement(1399..2793)
                     /gene="gltA"
                     /locus_tag="K8M77_001788"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBI7086279.1"
                     /translation="MSNLKKKRVKSREQDPIERGKNFKEVSLGYGEEEAIEEANRCLG
                     CKNPKCVEGCPVSVNIPSFISFIKKGDFSTSFEELSKYNALPAVCGRVCPQESQCEGK
                     CVLGIKGEPLAIGQLERFIADFARNNKLSPLKKSDKTLEKVAVIGSGPAGLTCAGELA
                     KLGYRVTIFEALHESGGVLVYGIPEFRLPKKDVVKYEIEEIKKLGVKIENNVVVGKTV
                     DIDELIENEGFKAVFIGSGAGLPKFMGIKGENANGVFSANEILTRVNLMKAFKNESHT
                     PLNLGKKVAVVGGGNVAMDAARTSLRLGAETHIIYRRSEKELPARLEEIHHAKEEGVI
                     LDVLTNPTEILTDEKGWVKGIKCIRMKLGEEDSSGRRRPIEIENSEFIMEVDSVIMSL
                     GTSPNPLISNATKGLKTNKRGCLLVDEESLKTSLKGIYAGGDAVTGAATVILAMGAGK
                     KAAKSIDSYLRKEE"
     gene            complement(2786..3685)
                     /locus_tag="K8M77_001789"
     CDS             complement(2786..3685)
                     /locus_tag="K8M77_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBI7086280.1"
                     /translation="MFKILEKRYLNDSVYLIKIEAPRVANKAKPGQFIILRTDEEGER
                     IPLTIADYNKEDKSVTIVVQGLGASTLELGKYNEGDYIHDFLGPLGKESDFIYEDLEE
                     LKKERILFVAGGVGAAPVYPQVKWLYNHGILADVIVGARNKDLLIFEKELREVSKNLY
                     IATDDGSYGFKGRVTDLLENLVKNKGINYNRAIVIGPMIMMKFMCILTKELNIPTTVS
                     LNPIMVDGTGMCGACRVTVGGEVKFACVDGPEFNGHLVDFDESIRRQAIYKTEEGRAF
                     LKLKEGNTHSHGGCGCRGDLKNE"
     gene            complement(3858..5024)
                     /locus_tag="K8M77_001790"
     CDS             complement(3858..5024)
                     /locus_tag="K8M77_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_022743700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBI7086281.1"
                     /translation="MARFTLPRDIYHGKDSLEVLKSLEGKKAFIVIGGGSMKRFGFLD
                     KVLSYLKEANMETKVFEGVEPDPSVETVMKGAKEMEEFNPDWIVSIGGGSPIDAAKAM
                     WIFYEYPDFTFEKAIVPFGLPKLRRKAKFVAIPSTSGTATEVTAFSVITDYKAKIKYP
                     LADFEITPDIAIVDPSLAETMPEKLVAHTGMDALTHAIEAYTASLRSNFTDPLALKAI
                     EMVNMHLVNSYKGNMEARGEMHEAQCLAGMAFSNALLGIVHSMAHKVGAVFHIPHGCA
                     NAIFLPYVIKYNRKACEDRYANIARHIGLKGESERELTDALIDLINKFNKELNIPSSM
                     KEYGIEEDDFKVNLKFIAHNAVLDPCTGSNPREIDDETMEKLYTCAYYGGDVDF"
     gene            5259..8120
                     /locus_tag="K8M77_001791"
     CDS             5259..8120
                     /locus_tag="K8M77_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7086282.1"
                     /translation="MAVAIVQQQQQQPNDKINIDLSNSNVAVVKNTNPPIKDEKESKD
                     KDNLKSDKKTEPKIEEKKQDKGNSKEEDKAQVKTEPKVKEDKDTKKSSEEIKEEVKNQ
                     KPVIKEVKLPVEKDSKNSSENLSNNSSDFASDVNRRLYEYESVRSNQSNAMNEAIRLH
                     NGDPSNTCVFFQGTCLRAIGVPVPSHIGYTSNLWNWLENNNWEMHRDFSNIQKGDIIF
                     AGEYHTMCFMGWKDKANGIAYVMGNEAYSYGDAYANRNLNGQAPTKENGWNRQYRATR
                     YYKYKGNSSKSNSDIVKTNIVTSKPVSSKDHKYTVEALKGTVIAKQDVYINEKPFPTT
                     EGIKPVGLANGGDKLTVTGRASNGWYEVLYNGQKAYISHRYTNFLEEKESKSKSSESI
                     TPLGVVKTNTSDKKSDSKEDNKKIENSLKDKDEKVNKGAESTEDKKVDESKDKATNKV
                     ESNKTKQAKPLESSKPVEETKKEESSKPSEIVTKTAFIKANGGLWLHSSKDTSYSSRV
                     SLMSNGAKVNVLEEDNSWFKVDYNGNTGWCSSKYVTNPVSYKSSTNKKVEENKSTEPK
                     KTMEENKPKKEAETSKPALTIVKTASVKANGGLWLHSSKDSYISSRLAIMDKGEKVTI
                     LEENGDWFKVNYNGKTGFCASKYLSDPTTYEKQSESNKIEEVKNTSPSKAVEESKPIA
                     PKKTVEENKPKKETETSKPTLTNIKRASVKANGGLWLHSTKDSSASSRITIMSNGEKV
                     DILDESGSWYKINYNGTMGWCPSQFLSNPTVISQSSQSKAVEENKPVYENKTVEVSKP
                     VNSTVKTAYIKANGGLWLHSTKDSYASSRISIMNKGSKIRVLEESGSWFKVDHNGNIG
                     WCSSEFLTNPVTSQNNTVEESKTVHLVQSNTNEASLRSAHVKANGGLWLHSSKDSSTS
                     SRLTVMGNGHKVEILEESGSWYKVRYNGNIGWCAKEFIA"
     gene            complement(8294..9208)
                     /locus_tag="K8M77_001792"
     CDS             complement(8294..9208)
                     /locus_tag="K8M77_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI7086283.1"
                     /translation="MDRVKSWEYDDIDNKFAIKDLDKSSFKRGSHIPIKVRKVFECED
                     IKYGDKKKITLIYKGEAFEAEIEGEIKGRIRMNWPKLIPKMKEVFKNEINDFEDSKEL
                     SVRVPRLKFTKETAIKYDVEMKTKYSHDVLCELGRLDIDIENKTIEEINEVGEVIERV
                     VKARKNQNKFREALFKRESKCKICGLAHKELLISSHIKPWSKSTPEEKLNPFNGFLLC
                     PNHDSLFDKHLISFRDNGEIIISKRLSEKEQELLNINKDIVINIEEENKKFLKEHRDV
                     FYEIEKAYDLEKELSISNKEIYKVELEV"
BASE COUNT         3223 a         1687 c         1191 g         3232 t
ORIGIN      
        1 atgtaaatac ttaaatgcgc ctattttaaa taatttactt tttatttatg tatttctaac
       61 tttataaaat ttaattaaag aaatagccct actttagaac ttaatctaaa atagggcaaa
      121 attatacctt agttatctga aaatttagct aaaagattta gtatttcagt gtataaccat
      181 attaaagtta ccattaatcc aaaagctcca taccattcca tgtactttgg atatccatat
      241 tgtgaagctt ttagtatgaa atcatagtcc attattaagc ttgctgtagc aattgcaaca
      301 acaaatagac taaatcctat accaattatt cctgaaccat atataggtaa agttattcca
      361 aagaaactaa gtaccattcc taataagtaa atagctccaa ttgatattat ggctatgaaa
      421 actcccctgc taattctgcc agctatatct ggagttcttc tatatataag tactgtagca
      481 actaagcata ttattgttaa aagcatagct gggaatacta ttccaggaaa catactttca
      541 aagtatcttg atatagaacc taaaagaaca ccttcaaaag cggcatagaa cactgctgta
      601 aactgtgcaa tctttggtat gaatgatgtt aataatccta aaattaatga tcctcctagt
      661 gaaaccacta aagcactttt ataattcaca tctggatttt gaaaagctat ccaactataa
      721 acaaaagcaa cacctaaaat agctgtcatt attaatattt ttactatagt tcctccaaca
      781 gtcatggttg ttccatcact aacagccttt tcaaatccag ctgctaatgc aggatttgat
      841 tctcttctca tgtttctacc tcctatataa atataagtaa tttattatag tttaaattat
      901 aaagtaaaag tatgtcaatt atgtttcatt tatggttata ctgcaaatta atattaattt
      961 aatgattaaa tttaattttt atagtatgtc ttatttactt tattataatc atatttcttt
     1021 aatattaatc ttcatctaga tcattccaac tgtgcagaca ataactccaa aggtgctcct
     1081 ttactatatt tctaccttta cattttggac attctttatt tttcttaaaa aataaaagtc
     1141 tttttaatat tcctatatta aaagtattcc cacaatcaat acaaataaaa ccataggtac
     1201 ttaatatcca aagtctaaat aaaagtattt ctagaaatac ataaattaaa aataaaataa
     1261 acttaaataa attaataggt tttaatatat aaaaaattat ggctgttgaa attactataa
     1321 gagtaaaaat taatagagtt aatttatttt ttttaaattt catatgaaac tcttccttaa
     1381 gaattgctaa atattatttt actcttcttt tcttaaataa gaatcaatgg attttgcagc
     1441 tttctttcca gctcccatgg caagaataac tgtagctgct cctgtaacag catctcctcc
     1501 tgcataaatt ccctttaagg aggtttttaa gctttcttca tctacaagga gacatcctct
     1561 cttatttgtc tttaatccct ttgtggcatt tgatattaaa ggattaggag aagttcctaa
     1621 ggacattatt acgctatcta cttccataat aaactctgaa ttttcaattt caattggtct
     1681 tcttcttcct gaagaatctt cctctccaag tttcatcctt atacacttta taccctttac
     1741 ccaacccttt tcatcagtta aaatttcagt aggattagtt aaaacatcta aaataactcc
     1801 ttcttcctta gcatgatgaa tttcttctaa tctagctggc aattcctttt cacttcttct
     1861 ataaattatg tgagtttctg ctcctaatct taaggaggtt ctagctgcat ccatggcaac
     1921 atttcctcca ccaactacag caaccttttt ccctaaattt aaaggagtgt ggctttcatt
     1981 tttaaaagcc ttcataagat ttaccctagt taaaatttca ttagctgaaa aaactccatt
     2041 tgcattttcc cctttaatcc ccatgaattt tggaagacct gctccagaac ctataaagac
     2101 agccttaaat ccttcatttt ctattaattc atcaatgtcc acagtttttc ctacaacaac
     2161 attattttct attttaactc ctagtttttt tatttcttct atttcatatt taactacatc
     2221 cttctttggc aatctaaact ctggaattcc ataaactaaa acccctccag attcatgtaa
     2281 ggcttcaaaa atagttactc tatatcctag tttagctagt tctcctgcac aggttaaacc
     2341 agctgggcca cttccaataa cagctacctt ctctaaagtc ttatcactct tttttaaagg
     2401 gcttaattta ttatttcttg caaagtctgc tatgaatctt tctagttgtc caatagctaa
     2461 tggttctccc tttattccta aaacacactt tccttcacat tgagattctt gtgggcaaac
     2521 tcttccacat acagcaggta aagcattata ttttgataac tcttcaaaag aagtagaaaa
     2581 atctcctttc tttatgaaag atataaaact tggtatattt acagaaacag gacatccttc
     2641 tacacactta gggtttttac accctaaaca tctatttgcc tcttcaatag cctcttcttc
     2701 tccatatcct agggaaacct ccttaaagtt tttacctctt tctattggat cttgctccct
     2761 agatttaact cttttctttt ttaaattact catttttcaa atcacctcta catccacatc
     2821 caccatgact atgggtattt ccctctttaa gttttaaaaa ggctcttccc tcttctgttt
     2881 tgtagatagc ttgtcttcta atagattcat caaaatccac taaatgccca ttaaattctg
     2941 gtccatcaac acaagcaaac ttaacttctc ctcctactgt aaccctacaa gcaccacaca
     3001 ttccagttcc atcaaccatt atgggattta aacttacagt ggtaggaata tttaattcct
     3061 ttgtaagaat gcacataaac ttcatcatta tcatgggtcc aattacaatt gctctattat
     3121 aatttatgcc cttattctta accagatttt ctaaaaggtc agttactctt cccttaaatc
     3181 cataacttcc atcatcagta gctatatata gatttttaga aacctcccta agttcctttt
     3241 caaagattaa taaatcctta tttctagccc caactataac atctgctaaa attccatggt
     3301 tatatagcca ctttacttga ggatatacag gtgcagcccc aactccacca gctacaaata
     3361 atattctttc tttttttaat tcctctaaat cttcatatat aaagtcactt tcttttccta
     3421 aagggccaag aaaatcatgt atatagtcac cttcattata ttttccaagt tctaaggtac
     3481 tagccccaag gccttgaaca acaatggtta cacttttatc ttccttatta taatcagcta
     3541 tggttaaagg aattctttcc ccctcttcat ctgttctaag tattataaat tgccctggct
     3601 tagccttatt agctactcta ggtgcttcta tcttaattaa ataaacagag tcatttaaat
     3661 atcttttttc taaaatttta aacatctaac taaacctctc caaaaatatt ttattctccc
     3721 acctaaaaaa ataggattgt atcaaagaac tcataaaatt tataactttt atctttgagt
     3781 tttaacacaa tcctcttatc tatactatat ttatctctct gctttatact ttccctaaaa
     3841 agataatctt ctcttcttta aaaatccaca tcaccgccgt aatatgcaca agtatataat
     3901 ttttccatag tttcatcatc aatctccctt ggatttgatc ctgtacatgg atctaaaaca
     3961 gcattatgag ctataaattt aagattaact ttaaagtcat cctcttctat gccatactct
     4021 ttcattgatg aaggtatatt taattcctta ttaaacttat ttattaaatc tattagggca
     4081 tctgttaact ctctttcact ttctcccttt aatccaatat gtcttgcaat gttagcatat
     4141 ctatcttcac aagcttttct attatattta attacatatg gcaaaaagat ggcattagca
     4201 catccatgtg gtatatggaa tactgcccct accttatgag ccatagaatg tactattcct
     4261 aaaagagcat tagagaatgc cattcctgct aaacattgag cctcatgcat ttctccccta
     4321 gcttccatat ttcctttata tgaatttact aagtgcatat taaccatttc aattgccttt
     4381 aaagctaaag gatctgtaaa gtttgatctt aaacttgcag tataagcctc aatagcatga
     4441 gttaaagcat ccattcctgt gtgagctact aatttctctg gcatagtttc tgctaagcta
     4501 ggatctacaa tagctatatc tggagttatt tcaaaatctg ctaaaggata ttttatctta
     4561 gccttataat cagtgattac tgaaaaggca gttacctctg tagcagttcc agaagttgat
     4621 ggaatagcta caaactttgc ttttcttcta agcttaggta atccaaaagg aacaatagcc
     4681 ttttcaaaag tgaaatcagg atactcgtag aaaatccaca tagcctttgc tgcatcaata
     4741 ggtgaacctc ctcctatgga aactatccag tctggattaa attcttccat ttcctttgca
     4801 cccttcataa cagtttctac tgatgggtct ggttctactc cttcaaaaac ctttgtttcc
     4861 atattagctt cctttaaata acttaaaacc ttatctaaaa agccaaatct tttcattgat
     4921 ccgccaccaa taactataaa agccttttta ccttctaagc tctttaacac ctctaaagaa
     4981 tcttttccat gatatatgtc ccttggtaaa gtaaatcttg ccatactaat aattcctccc
     5041 aaaataatat attcttaaca aaaaacaaag tagactatat aaactatagt ctactttaca
     5101 ttttatttat tattatttaa ttttattcct aaatttaaat taaaatttct tttacaatta
     5161 ttccattatt tattaacatt tctaattatg gtataattac atggaaattt aagtattata
     5221 gacactattt taaatttaag aaaataggag atgattttat ggctgtagca atagttcaac
     5281 aacagcagca acaaccaaat gataagatta atatagactt gtctaattct aatgttgctg
     5341 tagtaaaaaa tactaatcca cctataaaag atgaaaaaga atcaaaggat aaagataatt
     5401 taaaatctga taaaaaaacc gagcctaaaa ttgaagaaaa aaaacaagat aaaggtaact
     5461 caaaggaaga agataaagct caggtaaaaa ctgaacctaa agttaaagag gataaagaca
     5521 caaagaaaag ttctgaagaa attaaagaag aggtaaaaaa tcaaaaacct gttataaaag
     5581 aagtaaaatt acctgttgaa aaagattcaa agaattcttc tgaaaattta tctaataact
     5641 caagtgactt tgcttcagat gttaatagaa gattatatga atatgaaagt gttagaagta
     5701 atcaatctaa tgcaatgaat gaagctattc gtcttcataa tggagaccct agcaacactt
     5761 gtgttttctt ccaaggtact tgtttaagag caataggagt acctgttcca agtcatattg
     5821 gatacacttc taatctttgg aattggcttg aaaataataa ctgggaaatg catagagatt
     5881 tttcaaacat ccaaaaggga gatattatct ttgcaggtga atatcacaca atgtgtttca
     5941 tgggatggaa ggataaagca aatggcattg cctatgtaat gggaaatgaa gcctattctt
     6001 atggagatgc ttatgctaat cgtaatttaa atggacaagc tcctactaaa gaaaatggat
     6061 ggaatagaca gtatagagct acaagatact acaaatataa aggaaattct tcaaaaagta
     6121 attctgacat tgtaaaaaca aacattgtaa catcaaaacc tgttagctca aaggatcata
     6181 aatatacagt tgaagcctta aagggtactg taatagctaa gcaagatgtt tatattaatg
     6241 aaaaaccctt ccctactact gaaggaatta agcctgtagg actagctaat ggaggagata
     6301 aactaactgt aactggtaga gcctcaaatg gatggtatga agttctttat aatggccaaa
     6361 aagcttacat aagtcataga tatactaact tcttagaaga aaaggaatct aaatctaagt
     6421 cctctgaatc tattactcct cttggcgtag ttaaaactaa tacttcagat aaaaaatctg
     6481 attctaaaga agataataaa aagatagaaa attctcttaa agacaaagat gaaaaggtga
     6541 acaagggagc tgaatctact gaagataaaa aggtagatga atctaaggat aaagctacta
     6601 ataaagtaga atcaaataaa actaaacaag ctaaaccttt agaatctagt aagccagttg
     6661 aagaaactaa aaaagaagaa tcaagtaagc cttctgaaat agttacaaaa actgccttta
     6721 taaaggctaa tggtggttta tggctacatt ctagtaaaga tacttcatac tcttctagag
     6781 taagcctaat gagtaatgga gctaaggtta atgttttaga ggaagataac tcttggttta
     6841 aggttgacta taatggaaat actggttggt gttcaagtaa atatgtaact aatccagttt
     6901 cttataaaag ttcaactaat aaaaaagtag aagaaaataa atctacagaa cctaaaaaaa
     6961 ctatggaaga aaataaacct aagaaagaag ctgaaacaag taaacctgct ttaacaattg
     7021 ttaaaactgc ttctgtaaaa gctaatggtg gactatggtt acactcttct aaggattcct
     7081 atatctcttc tagattagca attatggata aaggtgaaaa agttacaatc ttagaggaaa
     7141 atggagattg gtttaaggtt aactataatg gtaaaactgg cttctgtgca agtaagtact
     7201 taagtgatcc tactacttat gaaaaacaat ctgaatctaa taaaatagag gaagttaaaa
     7261 atacctctcc tagtaaggct gtagaagaaa gtaaacctat tgcacctaag aaaactgtgg
     7321 aagaaaataa gcctaaaaag gaaactgaaa caagtaaacc tactttaaca aatattaaaa
     7381 gagcctctgt aaaggcaaat ggtggtttat ggctacactc tactaaggat tcctctgcat
     7441 cttctagaat taccataatg agtaatggag aaaaggttga tattttagat gaaagtggtt
     7501 cttggtacaa gattaactat aatggaacta tgggttggtg cccaagtcaa ttcttaagta
     7561 acccaactgt tatctctcaa agctcacaaa gtaaggctgt agaggaaaat aaaccagttt
     7621 atgaaaacaa aacagttgaa gtaagtaaac cagttaatag tacagtaaaa acagcttaca
     7681 taaaagcaaa tggaggttta tggttacatt ctactaaaga ctcctatgct tcttctagaa
     7741 taagcataat gaacaaagga tcaaagataa gagttttaga agaaagtggt tcttggttta
     7801 aggttgacca caatggaaat ataggttggt gttcaagtga attcctaact aatccagtaa
     7861 catcacaaaa taatactgtg gaagaaagta aaacagttca tctagttcaa tcaaatacta
     7921 atgaagcttc attaagatca gcccatgtaa aagctaatgg aggattatgg ctacactcat
     7981 ctaaagactc ctctacttca tctagattaa ctgtaatggg taatggtcat aaggttgaaa
     8041 tcttagaaga aagtggttct tggtacaagg ttagatataa tggaaacata ggttggtgtg
     8101 ctaaagaatt tatagcttaa aagaaattgg tacttattat aattaatttt cagggtactt
     8161 tatagttttt ttcttataaa taccataaat acttttaaag gcccataatt taaaatttct
     8221 ctttaaatta tgggcccatt ttctctttaa aagcaaaaaa actactccat tccaactttt
     8281 atgccatatg tatttaaact tctaattcaa ccttatatat ttccttattg cttattgaca
     8341 actctttctc taaatcataa gccttctcta tttcataaaa gacatctcta tgctccttta
     8401 aaaacttctt attttcctct tcaatattaa tcacaatatc cttatttata tttaatagct
     8461 cctgctcttt ttctgagagc cttttagata ttatgatttc tccattatcc ctaaagctta
     8521 ttaaatgctt atcgaataag gaatcatgat ttgggcatag taagaatcca ttaaagggat
     8581 ttagcttttc ctctggtgta cttttgctcc aaggttttat atggcttgat attaaaagct
     8641 ccttatgagc tagtccacaa attttacact tactttctct tttaaatagt gcttctctaa
     8701 acttgttttg attttttcta gccttaacaa ccctctctat aacttctccc acttcattta
     8761 tttcttctat ggttttgttt tcaatgtcta tatctagcct tcctaattca caaagaacat
     8821 catgactata tttagttttc atttctacat catattttat ggctgtttcc tttgtaaatt
     8881 tcaatctagg tactcttaca ctaagctctt tagaatcttc aaagtcattt atttcatttt
     8941 taaaaacttc cttcatttta ggtattaact ttggccaatt cattcgtatt ctacctttaa
     9001 tttccccttc tatttcagct tcaaaggctt cacctttata tataagagtt atttttttct
     9061 tatctccata tttaatatct tcacattcaa aaacctttct aacctttatt ggtatatggc
     9121 ttcccctctt aaatgaggat ttatctaaat cctttatggc aaatttatta tcaatatcat
     9181 catactccca tgatttaact ctatccaaag taactcttcc cccactcatt aaaataatct
     9241 atgtttctat aaatttatat ttccaagtca tcttagttaa aacaactaaa aactatgtta
     9301 ccatttctta ctccacattc tataattata caa
//
LOCUS       DAEVGI010000095         9207 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEVGI010000095 DAEVGI010000000
VERSION     DAEVGI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.095"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(5..1459)
                     /locus_tag="K8M77_001793"
     CDS             complement(5..1459)
                     /locus_tag="K8M77_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBI7086284.1"
                     /translation="MNETINYDSLLILSVVAFFTPFLVSRLKRVNIPYQVGEIFIGIL
                     LGKSFLNIIKPDIWILFLSNLGLAYLMFLSGLAIDFDELKPKEGELLKDSKLFTSIKM
                     FIVSIITAIILSFTLKFAGISEGYIFFALLFTAAAPGLLVPILKTKHIINSSYGQTLL
                     VFGIITQLVSLIGVTFVASVAVNGITLKSFTFLIIFAVAIVVYFLSKIIFKVHDFSTI
                     AFKNLHLSVRGAFVLVLVLVAVAEKVDSEIILGSFLAGMVFSLIVGKAKEEISHQLDV
                     VGYGFLIPIFFIMVGVNVDLKAVVENPQSIAKIPLFILIFFLVKFIPSLLLKKKYGTR
                     NSLAASMLLTAQLSLVIVGSQMALNLGYITAADYSAFVVTTVITCIFFPILFEKLIAL
                     HGDTNDAVEEDEKIIIREVLVSSQWYIGKALKDCKLPTGCRIFSMVRDGHEFMPNANT
                     TLEENDLVILAGVKHDVNETINMLSTCDLTLPEE"
     gene            complement(1488..2108)
                     /locus_tag="K8M77_001794"
                     /pseudo
     CDS             complement(1488..2108)
                     /locus_tag="K8M77_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474694.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            2346..3131
                     /locus_tag="K8M77_001795"
     CDS             2346..3131
                     /locus_tag="K8M77_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HBI7086285.1"
                     /translation="MRSNYHTHTYRCNHATGTIEDYVKEAIKANIDEIGISDHLPHPG
                     KNIDNYHRMAYEDLEDYFGKIEKVIEKYGDKISIKRSIECEYFPDFGWLYEELKDKYK
                     ADYLALGVHFFPYKGEWVYVGTIELTPEILMIYADFVVEAIKSGYFSYLAHPDLFGMT
                     YKNWDVHAEEASKRILKAAEEMNMPIEINVNGMRKNKIYYNNGERYQYPIKEFWELSK
                     EYNVKRIVGIDAHNPEEMHDLDMGLNFAKEHDLTIIDRLEFNK"
     gene            complement(3174..4193)
                     /locus_tag="K8M77_001796"
     CDS             complement(3174..4193)
                     /locus_tag="K8M77_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBI7086286.1"
                     /translation="MNKSIAYIGTYTNGASKGIYRLCLDTAKGNIEDLSVVARFGNPT
                     YLCIHNNKLYTVGNPISLDTLGGVASYNIEEDYSLKLTGASLLQGKKPCHINIIPDKS
                     LIVSSNFHEKSINTYSLNENFDIDTSLSAFSHKDDSKMHFASTTPDNKFICAVNLGMD
                     RIELFKINSNNTLSYIENLSFYCTKGCGPRHIEFSKNGKFAYVICENSSEIIILKYLG
                     EEGFKLVQYLHVLPNGFGGQNFGSAIKISPCNKFLYVSNRGFNGISAFRINEETGSLS
                     LINHYSSHGDFPRDFEISPCNKFLVIANEKSDNLTIYLKNPDGTLKLFKDDIFIPSPT
                     CIKFK"
     gene            4393..6333
                     /locus_tag="K8M77_001797"
     CDS             4393..6333
                     /locus_tag="K8M77_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HBI7086287.1"
                     /translation="MKKTIGILAHVDGGKTTFSEQLLYHTKSIRNRGRVDHKNSYLDN
                     NEIEKDRGITIYSEVGKFSIENQEYYLIDTPGHIDFSPEMERAISVLDYAILIISAVE
                     GVQGHSETIWELLNKYKIPTFIFINKIDREGAEVNKVINEMKHKLSEDIIFFSSELED
                     ETIEEVVERDEDLLNLYLEGNLSEEELLNKIPSMIKELKIFPCLCGSALLDEGVEDFI
                     SWFHNLSFTNYEESEDSFKGRVFKVRHDEKGNRLTFIKALSGILRTKEELTYLKEGKE
                     FLEKVNEIRIYNGSKYELVNEVKAGDVFAVVGVKGLESGDGIGIENIDSYDMVPTLKS
                     KVVYREGLNPKEVLSWFKILESEESTLSISWDERLKEIHVNIMGKVQLEVLKEVMKNR
                     FNEEIEFGTPEILYKETLNEEVIGYGHFEPLGHYSEVHLKIEPLERNSGIVFENKCHV
                     DDLTVGNQNLIRTHIFECEHKGILTGSPITDLKITLLTGKAHNKHTSGGDFREATKRA
                     LRQGLESGENKLLEPYYKFKIDVDLNLIGRVMNDIQKMHGEFKDPIIDGERAIIEGKG
                     PVSTFINYGMEFQSFTKGKGGLSLKFHGYDVCHNEEEIIKKRAYDRNADIDYTSTSIF
                     CSKGQAYLVKGEAAKEHMHCLV"
     gene            6495..6848
                     /locus_tag="K8M77_001798"
     CDS             6495..6848
                     /locus_tag="K8M77_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086288.1"
                     /translation="MELVNIYDEYREVNKNYVDFIEELVNKNFEGFSEDFVMSNLENF
                     QNSIGDLKVKADDIQVEEENKDNLKDLKYLIVDTLFLTFDLNNFYKLKEFERFKMRFA
                     NYVNKRRRDEMLKSF"
     gene            complement(6949..8946)
                     /locus_tag="K8M77_001799"
     CDS             complement(6949..8946)
                     /locus_tag="K8M77_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBI7086289.1"
                     /translation="MSTNKSSIEKITLGSFLIALGVVYGDIGTSPLYVMKSIINGNGG
                     LESITPDFILGVLSLIFWTMTLLTTIKYVLITLKADNKGEGGIFSLYTLIRRRAKWLI
                     IPAMVGGSALLADGMLTPVVTVTSSIEGLKILPSFNDIFGNNQDIIIIIVLVILSFLF
                     FIQHFGTEIIGKIFGPVMFIWFAFLAILGIVNLSGNLYLLKALSPHYAIKILFSPNNH
                     LGFFILGGVFLSTTGAEALYSDLGHVGRKNIYLTWPLVKICLLLNYFGQAAWILSQKN
                     NAKLYGIESLNPFFQMMPSWLLLFGVLISTLAAIIASQALISGSYTLVSEAIKLNLFP
                     RLQCLYPSNSKGQIYMPAINRILWIACIAIVLYFRSSDNMEAAYGLSITVTMLMTSIL
                     LFNYLLKRKTPLPIALIILVFFSSLEFSFLISSAVKFEKGGFVSVLIALCILSIMYIW
                     IKGHYIKMSLLDYIPIENYKNQLKELKNDVDRPKYATNLVYLTSSEKSKRIERKIMYS
                     ILDKRPKRADVYWFVNVIVTDEPYTSEYTVNTFGTDYMVKVQLKLGFRVNQKLNVFLR
                     QIVCELINNGDIKVQNKKYTTLHNRNVGDFRFILINECLSSESKLESWNSIIIRSKLF
                     IKKFTVSPAKWFGLESSEIEVENVPLILGSTEHTTLKRVYK"
BASE COUNT         3551 a         1108 c         1401 g         3147 t
ORIGIN      
        1 ctttttattc ttctggtaaa gttaaatcgc aggttgaaag catattaatg gtttcattta
       61 catcatgctt aactccggca aggattacta agtcattttc ttctagagtt gtattagcat
      121 taggcataaa ctcatggcca tctcttacca ttgagaaaat cctacatcct gtaggtaact
      181 tacaatcctt taaggcttta cctatatacc attggctact aacaagtact tcacgtatta
      241 ttattttttc atcttcttct acagcatcat tagtatcacc atgaagtgca attagttttt
      301 caaataatat tgggaagaag atacaagtta ttactgtggt aactacgaaa gcagagtagt
      361 ctgcggcagt tatgtatcct aaattcaatg ccatttgaga accaactata actaaactaa
      421 gctgtgccgt taataacata gatgctgcta aggaatttct cgttccatat ttctttttaa
      481 gtaaaagtga tggaatgaat tttactaaga agaaaattag tataaacaaa ggtattttag
      541 caattgattg aggattttca acaactgcct ttaaatcaac attaactcca accattataa
      601 agaatatagg aattaaaaag ccatacccaa ccacatctaa ttggtgagaa atttcttctt
      661 tggctttccc aactattaat gaaaatacca taccagctaa gaatgagcct agaattatct
      721 cagagtctac tttttcagca acagcaacaa gtaccaaaac aagaacaaag gcacctctta
      781 cgctaaggtg aaggttttta aaggcaatag tagaaaaatc atgaacttta aatataatct
      841 ttgataagaa atatactact atagccactg caaatattat taagaaagta aaactcttta
      901 aggtaatacc atttactgca acagaagcca caaaagtaac acctattaaa cttactaatt
      961 gagttattat tccaaaaaca agtaaagttt gaccataact actatttatt atgtgttttg
     1021 tttttaaaat aggtacaagt aatcctgggg cagcggcagt aaatagcagt gcaaagaata
     1081 tatatccttc tgaaatacct gcaaatttta atgtaaagga taaaattata gctgttatta
     1141 tagatacaat aaacatcttt atactagtaa ataacttaga atcttttaaa agttctcctt
     1201 ccttaggttt aagctcatca aagtctatag caagaccact taaaaacatt aaataagcta
     1261 atcctaagtt tgataaaaat aaaatccaaa tatctggttt aattatgttt aaaaaacttt
     1321 tacctaataa tatacctata aatatttccc ctacttgata cgggatattt actcttttta
     1381 gtcttgaaac taaaaaggga gtaaaaaagg ctactacaga taagattaac aatgaatcat
     1441 aatttatagt ttcattcata aataaatcat ctcttttctt tagtatttta tattaaattt
     1501 aaataactaa gttcatatat aaggattttt tctcttgagt catatttata cttattagga
     1561 atctttattt ccttctttaa agaaaatgga gtgtagtttt ctaaatagta tatgtaatca
     1621 tcagagggat aataaagtat aatatctact ggtctctcat gattagatac agaatcaaga
     1681 atatttccaa cgacttttat aaatatttgt tctgaaaagg gattaaaaaa ataaaattta
     1741 ttatcctttg aatctatagg atacctttca gcaaaacaat ttataaaatt taatttatct
     1801 tacttaactt tattttgtga taaataagat tttttatttc ctaagcattc attaaaaaag
     1861 aagttattca tctctattcc tgtaacttta atatttaaaa agtaatttaa atagaaatta
     1921 agtcttcctt taccacagcc aaaatcgatt aaggaatctt ctttagaaaa ttcatattga
     1981 gaacacatag cttctaatac atcataagat gtaggttcat acctattgta atgtagagat
     2041 tcattaaata ttttttgttc tccagaagtt tttatattta gcaatttttc aaaatattgt
     2101 tctttcattt tctcctcttt cataaaaatt tagtatttaa atttacaatt atcagtatag
     2161 tattttacct taaatagggc aaggtaaaat ttagggtgag gaattcttag ttaatcgaaa
     2221 atactttagg taaattaaac taaagaagag ataatagatt tatagaatgg aaatctaatg
     2281 ttggtcaaac ttttataatt agatacaata ataaatgaat gtttaaatgc ataggaggag
     2341 ttttaatgag aagcaattat catacacata catataggtg taatcatgcc acaggaacta
     2401 ttgaagatta tgttaaagaa gctataaaag ctaatataga tgaaattgga atttctgatc
     2461 acttaccaca tccaggtaag aatatagata attatcatag aatggcttat gaagatttag
     2521 aggattactt tggaaagata gaaaaagtta tagaaaagta tggagataaa atatcaataa
     2581 aaagatctat agaatgcgag tatttcccag acttcggatg gctttatgaa gaattaaagg
     2641 ataaatataa ggcagattat ttagctttag gagtacattt tttcccttat aaaggagaat
     2701 gggtgtatgt tggaacaata gaattaacgc cagaaatcct aatgatttat gctgattttg
     2761 tagtggaagc aattaagagt ggatattttt catatttagc acatccagat ttatttggaa
     2821 tgacatataa aaattgggat gtgcatgcag aagaggcctc aaagagaatt ttaaaagctg
     2881 ctgaagaaat gaatatgcct atagagataa atgtaaatgg aatgagaaaa aataaaattt
     2941 attataataa tggagaaaga tatcaatatc caataaaaga gttttgggaa ttatctaagg
     3001 aatataatgt taaaagaata gttggaattg atgctcataa tccagaagaa atgcatgatt
     3061 tagatatggg gttaaacttt gctaaagaac atgatttaac tattatagat agattagaat
     3121 ttaacaaata aaaaaggagt taaagctcct tttttattat atgaaattat ttcctattta
     3181 aattttatac atgtaggaga tggaataaat atatcatctt taaaaagttt tagtgttcca
     3241 tctggatttt ttaaatatat tgttaggtta tctgattttt catttgcaat tactaagaac
     3301 ttattacatg gactgatttc aaaatcccta gggaaatcac catgtgaact atagtgattt
     3361 ataagtgata aagaaccagt ttcctcattt attctaaagg ctgatattcc attaaagcct
     3421 ctgttagaaa cgtataggaa tttattacaa ggacttattt ttattgcaga accaaaattt
     3481 tgtcctccaa agccattagg aagtacatga agatattgaa ctaatttaaa tccttcttca
     3541 cctaaatatt ttaatataat tatttcagaa ctattttcac atataacata tgcaaacttt
     3601 ccattctttg aaaattctat atgtcttgga ccgcatcctt tagtacaata aaaacttaga
     3661 ttttcaatgt aacttaaggt gttattagaa ttgattttaa aaagttctat tctatccata
     3721 cctaaattta cagcacatat aaatttgtta tctggagttg ttgatgcaaa atgcattttt
     3781 gagtcatctt tatgagaaaa tgcacttaat gaagtatcta tatcaaaatt ctcatttaat
     3841 gaatatgtat taattgattt ttcgtgaaaa ttactagaaa ctattaagga tttatctggg
     3901 attatattaa tatgacaagg ttttttacct tgaagtaaac tagcccctgt taatttcaat
     3961 gaataatctt cttctatatt ataagaagca acaccaccta gtgtatctag tgaaattggg
     4021 ttacctactg tatatagttt attgttatgt atgcacaaat aagtaggatt accgaatcta
     4081 gctactacgg ataaatcttc aatatttcct ttagcagtat ctagacataa tctatagata
     4141 cctttactgg ccccatttgt gtaagtacct atatatgcaa tagatttgtt catttttaaa
     4201 gccccctctg acttgattat attgttaatt ttacgtttta tttgtatgat ataagatata
     4261 ttaacaattt ttataaataa tattaaagat taaatattat ttgttatacc atataaaaca
     4321 aaaatagtaa agaatataaa ataaaaacat atataatttt taatgtttag atttttttaa
     4381 tggaggacaa gtatgaaaaa gactattggt atattagctc atgttgatgg aggaaaaaca
     4441 actttttctg agcaactttt atatcataca aagagtataa gaaatagggg aagagttgat
     4501 cataagaatt cttatttaga taataatgaa atagagaaag acagaggtat aactatatat
     4561 tctgaggtag gtaaattttc tatagaaaat caagaatatt atcttataga tactccaggg
     4621 catatagatt tttcaccaga aatggaaaga gctattagtg ttttagatta tgctattttg
     4681 attataagtg cagtagaagg ggttcaggga catagtgaaa caatatggga attattaaat
     4741 aagtataaga ttcctacttt tattttcata aataagattg atagagaagg agcagaggta
     4801 aataaggtta taaatgaaat gaaacacaaa cttagtgaag atattatttt cttttcaagt
     4861 gaattagaag atgagactat agaagaagta gttgaaagag atgaggactt attaaactta
     4921 tatttagaag gaaatttaag tgaagaagaa ttattaaata aaataccaag tatgataaag
     4981 gaacttaaaa tttttccttg tttatgtggt tctgctttac tagatgaggg cgtagaagat
     5041 tttataagtt ggtttcataa cttatcattt actaactatg aggaatcgga agattctttt
     5101 aaaggaagag tttttaaagt aagacatgat gaaaagggaa atagattaac ctttataaaa
     5161 gctctaagtg gaattttaag aactaaggaa gaattgacat atttaaaaga aggaaaagag
     5221 tttttagaga aagtaaatga aattagaata tataatggaa gtaaatatga acttgtaaat
     5281 gaagttaagg caggagatgt atttgcagtg gtaggagtta aaggattaga atctggtgat
     5341 ggaattggta tagaaaatat tgattcatat gatatggttc ctactttgaa gtctaaggtg
     5401 gtttatagag aagggttaaa tccaaaggaa gtactttcat ggtttaaaat cttagaaagt
     5461 gaagagagta ccttaagtat atcttgggat gaaagattaa aagaaattca cgttaatatt
     5521 atgggaaaag ttcaattgga agttcttaaa gaagttatga aaaatagatt taatgaagaa
     5581 atagaatttg gaactccaga gatattatat aaagagacat taaatgaaga agtaatagga
     5641 tatggccatt ttgagccttt aggacattat agtgaggttc acttaaaaat tgagccttta
     5701 gaaagaaatt caggaatagt atttgaaaat aaatgccatg tagatgatct tacagttgga
     5761 aatcaaaatt taataaggac tcatatattt gaatgtgagc ataaaggaat attaacaggt
     5821 tcgcctatta cggatcttaa aataacctta ttaacaggaa aagctcacaa caaacacaca
     5881 agtggtgggg attttagaga agctacaaag agagctttaa gacagggatt agaaagtgga
     5941 gaaaataaac ttttggagcc ctattataag tttaaaatag atgtggatct taacttaata
     6001 ggaagagtaa tgaatgatat acaaaagatg catggggagt ttaaggatcc aattatagat
     6061 ggagagagag caatcataga aggaaaggga cctgtttcta catttataaa ttatggtatg
     6121 gagtttcagt catttactaa gggaaaggga ggactttctc ttaagtttca tggatatgat
     6181 gtatgtcaca atgaagaaga gattataaaa aagagggcat atgatagaaa tgcagacatt
     6241 gattatactt ctacttctat attttgttca aagggtcaag cttatttagt taaaggagaa
     6301 gcggcaaaag aacatatgca ttgtttagtt tagaaaaatt ttgtcttata ttgtttttta
     6361 gaacctttaa aggaatatat gagattaaaa tttagctaaa ttatattcat tagacaaaaa
     6421 tatatagctt agttataatt tatagagaaa gtttaaacta ttaaagatga attaattttc
     6481 tatgaggtga agaaatggaa ttagtaaata tttatgatga atatagagaa gtaaataaaa
     6541 attatgtgga ttttatagag gaactagtaa ataaaaattt tgaaggattc agtgaagatt
     6601 ttgtaatgag taatctagaa aactttcaaa attccattgg agatttaaaa gtaaaggctg
     6661 atgacattca ggttgaggaa gaaaataaag ataatttaaa ggatttaaaa tatttaattg
     6721 ttgatacatt attccttacc tttgatttaa ataactttta taaacttaaa gaatttgaaa
     6781 gatttaaaat gagatttgca aattatgtta ataaaagaag aagagatgag atgttaaaat
     6841 ctttttagga taaaaagagg gcttttatca agaaataaca aaacttacaa acaatatgaa
     6901 aaaatactgt ttgtaagttt tatgaaaagt tattttgata aaagtccttt atttataaac
     6961 acgttttaga gtagtatgtt cagtagatcc taatattaaa ggaacatttt caacttcaat
     7021 ttcactagat tcaaggccga accatttagc tggagaaact gtgaattttt ttataaaaag
     7081 cttagatcta attataattg aattccatga ttcaagcttg ctttctgatg ataaacattc
     7141 atttataaga ataaatctaa aatctcctac atttctatta tgaagtgtag tgtacttttt
     7201 attttgaacc ttaatatcac cattatttat aagttcacac actatttgac gtaaaaatac
     7261 attaagcttt tggttaaccc taaaaccaag tttaagttgc accttaacca tataatcagt
     7321 accaaaggta tttaccgtat attcagatgt ataaggttca tcagttacaa ttacatttac
     7381 aaaccaatat acatctgctc tcttaggcct tttatctaaa atagagtaca ttatttttct
     7441 ttctattctt ttagattttt ctgaacttgt taaataaaca agatttgttg catatttagg
     7501 tctatctaca tcattcttta attcctttaa ttgattttta taattttcaa taggaatata
     7561 gtctaataga ctcattttta tataatgacc ttttatccat atatacatta tgcttaaaat
     7621 acataaggct attaatactg aaacaaaacc acctttttcg aatttaactg cacttgaaat
     7681 taagaatgaa aattctaaag agctaaagaa gactagtata attagtgcaa ttggtaatgg
     7741 ggtttttctt tttaataggt aattgaataa aagtatggat gtcataagca ttgttacagt
     7801 tattgataat ccataagctg cttccatatt atctgaactt ctaaaataca aaactatagc
     7861 tatacaagct atccaaagta ttctgtttat agctggcata taaatttgtc ccttagaatt
     7921 agaaggataa agacattgta atctaggaaa taaatttaat ttaatagctt ctgaaactaa
     7981 ggtatatgaa cctgaaatta aagcttgaga tgctataata gcggctaatg tagaaattaa
     8041 aactccaaat agtaaaagcc aactaggcat catttgaaaa aatggattta agctttcaat
     8101 tccatataat ttagcattat ttttctgaga taatatccaa gcagcttgac caaaataatt
     8161 taatagtaag catattttaa ctaaaggcca agtaagatag atattttttc taccaacatg
     8221 accaagatca gagtacaagg cttcggctcc agttgttgat aaaaatactc ctccaagaat
     8281 aaaaaaacct aaatggttat ttggactaaa taaaattttt attgcataat gaggagaaag
     8341 tgcttttaaa agatataaat ttcctgacaa atttacaatt cctaatatgg ctaaaaatgc
     8401 aaaccaaata aacataactg gtccaaagat tttacctatt atttctgtgc caaaatgttg
     8461 aataaagaac aaaaaactta gtattactag tacaattatt ataataatat cttgattatt
     8521 tccaaatata tcattaaaac taggtaagat ttttaaacct tcaatggatg aagtaacagt
     8581 tacaacaggg gttagcattc catcagctag tagagcagaa cctcctacca tggcaggtat
     8641 aataagccac ttagcacgac gacgaattaa ggtataaaga gaaaatatac ctccttctcc
     8701 cttattatca gcctttaatg ttataagaac atactttatt gttgttaata aagtcatagt
     8761 ccaaaaaata agagataata ctccaaggat aaaatcagga gtaatgcttt ctaaacctcc
     8821 attgccattt attattgatt tcataacata aagaggagaa gttccaatat ctccataaac
     8881 aactcctaag gctattaaaa agcttcctaa agtaattttt tcaatagaac ttttattagt
     8941 actcatattt attcttcctt tattatgata agttaattat taagcttaaa atattaataa
     9001 attattaatg tgtacacata aatttaaaat ctaataaaat ttatactagc accattatga
     9061 aatgatgtaa agttgtaaat taaaaagaaa ttagttctat tcaataataa tgtttacatt
     9121 agcaagttta ttaaaaaata catgagtttt ttgaaaaaat tataatgaat ttctgatgag
     9181 tattaaagta atctagtaaa ataaaaa
//
LOCUS       DAEVGI010000096         8894 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEVGI010000096 DAEVGI010000000
VERSION     DAEVGI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.096"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            103..342
                     /locus_tag="K8M77_001800"
     CDS             103..342
                     /locus_tag="K8M77_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBI7086290.1"
                     /translation="MNDNQVVSFRDENGKKIEYLVLEQKLICGAEYVAMAPKNDKTHV
                     ELFKINFDKDWNETLDSVESEKEINMFKQVSNLKF"
     gene            717..1541
                     /gene="hisJ"
                     /locus_tag="K8M77_001801"
     CDS             717..1541
                     /gene="hisJ"
                     /locus_tag="K8M77_001801"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBI7086291.1"
                     /translation="MILNTKRDGHVHSTYCPHGSNDSLDMYIDTALRNGITEITFTEH
                     MPLPMEDPSPRKNSALPMERLEEYIKGITELKEKVKDKIKVNVGLEVDYVEGYEEETK
                     AILNKYGKYLDDAILSVHIVKFQGEYYQIGNGSDMIGELVEKIGGMDKLYDLYYETML
                     KSIKCDLGAFKPKRIGHPTLVRKYKADFPNEKYNIKLLENILKEMKKGNYEIDLNTSG
                     LRRAKCGEVHPQGIFLDLCLKYNIPMVLGSDSHSSKYVGVEFEKVANMIFPDSEAI"
     gene            complement(1668..3086)
                     /locus_tag="K8M77_001802"
     CDS             complement(1668..3086)
                     /locus_tag="K8M77_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI7086292.1"
                     /translation="MSKYDKKLKKALAQYENGNYEEALDICEKILDKDYNNEEALTLE
                     SRALEKLNRISDAIVSWKINAEYNNNEEARTKLDKFNNLDEKKLAMSTIFSETMKDEI
                     KARLQEEAQKEYAKQLEKREPEIKPLSNDTEKEKENKKVEQAPVIKINKEKPREDVKI
                     DDIKPLDYKIKETSKNESNHLDNVTKVDFHKKDEIKKSKDKPKDDEPKVISTPNFKNE
                     KSNAKKSNKKKPVKYIAVAVSGAIVLLLAYGVTKNIKTTDASKNQITASEEANNNSNK
                     TENSTDNKTNEDSKKETKTITADELNKAISNNDINSLYTLLTSTPKDKVPQDALDAYN
                     KAEKLMKTDGVKDLYLKGSDLFKEKKYEDALKDFEKAYAFSSDSYLRPHLIYFMGTSY
                     ENLDKNTEAIKYFQEYLKDYKAKPDAEDFMYTPQCLYNLAILYNKEGNSAESKKYAQE
                     IENDYPNTMFYNDVTKKIIYGK"
     gene            complement(3288..5195)
                     /locus_tag="K8M77_001803"
     CDS             complement(3288..5195)
                     /locus_tag="K8M77_001803"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBI7086293.1"
                     /translation="MDFIKVGAASPKLKISDTKYNLKEIETMILNAIEEEVKVLVFPE
                     LSISAYSCGDLFTNEKLIENCNLAIKNLVEFSKGKDILIAVGAPFLYRYSLYNCAYII
                     LNGKLLGIVPKSYIPNYSEFYEKRWFTEGFNIKGLNVDLDFQKEVPFGTDLIFSFKNL
                     KVGFEICEDLWVTIPPSSNLALMGANLICNLSASNELVSKSSYRKSLVQNQSARTMCS
                     YIYSSAGVHESTTDLLFSGHMIIAENGTIIKENNRFKRENDLLTGIVDLFKLDAERIK
                     NISFRNSTFNENNEPRFIPFNLENTEIKNFDREIDKHPFLPKSQYAMEERCEEILNIQ
                     AAALAKRLEHTNLKKAVIGISGGLDSTLALLVVVKTFDMLNIPRENIITITMPGFGTT
                     DRTYNNAVTLCKELHCDFREINIVNAALQHFEDIGHDKDIHDVTYENVQARERTQILM
                     DLANKEGGLLIGTGDLSELALGWCTYNGDHMSMYSVNCSIPKTLVRFLVNYFANHEIS
                     NDAKEALLDILDTPVSPELLPKDKEGKIAQKTEDIVGPYELHDFFLYHFIKHGSSPER
                     ILFLAKEAFKNDYDEETLKKWLDKFIRRFFTQQFKRSALPDGPKVGSISLSPRGDWRM
                     PSDAVYNDFLI"
     gene            5509..5922
                     /locus_tag="K8M77_001804"
     CDS             5509..5922
                     /locus_tag="K8M77_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBI7086294.1"
                     /translation="MLWLWVVVILAGIIIDYLSSDVLFVGFSFGALGGLVLAFLDVNV
                     ILQFILFSIITILFFFYVYPKIKRKIKADNLGTKTMEQSYIGRKIILDENVEDSSLIK
                     FEGIYWTFKSVSGALKKGTEVEIIGIEGNKILIKK"
     gene            5942..6892
                     /locus_tag="K8M77_001805"
     CDS             5942..6892
                     /locus_tag="K8M77_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI7086295.1"
                     /translation="MVATVIIIILLLIVVFAAISSIKVVNTGYVYVLERFGQFSKILE
                     PGWHLVIPFADFVRKKISTKQQILDIPPQYVITKDNVKIEIDNVIFYKVLNAKDAVYN
                     IEDFKSGIVYSTITNMRNIVGNMSLDEVLSGRDKINLELLTIIDSITDAYGIKILSVE
                     IKNIIPPAEIQDAMEKQMKAERDKRATILQAEGLKQSEIARAEAEKQAKILRAEAEKE
                     ANIRHAEGLKESQLLEAEGKARAIEEVSKAEAAAIERINTSIIESGTNEVVIALKQVE
                     ALKEMAANPANKLILPNEAVSSLGSIAAIADILKENKENK"
     gene            7072..8124
                     /locus_tag="K8M77_001806"
     CDS             7072..8124
                     /locus_tag="K8M77_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBI7086296.1"
                     /translation="MSNYFWCDVNLKNIENNINVIRDLIQNKKLIAVIKGDAYGLGSK
                     KIAEFIEDKVDIIAVGNIYESLCLDNINKDILILSPLCTKDDFKDERDNLILTLDNEE
                     ILNELDKETKKRVHIYVDTGMNRMGIKPNKLDSFIERVKNEFPNLEIDGIYTHLHNAK
                     YVNYTLKQIELFKSTVEKYRDKVRIIHCMASSAIINDQLRKACDFTTGARAGNVLYGY
                     IGFNKGIKKTYEYYAKPVNTYKVSKGQTVGYGCLFKADKDMEIGIIECGNVHGLGISR
                     EIKNNVFYDIFRMIYRRFKERSVIFNNNVPVKILGKVNMNITIISMENCSKDSVLKVD
                     ISPIISDSSIEKRYIY"
     gene            8341..8841
                     /locus_tag="K8M77_001807"
     CDS             8341..8841
                     /locus_tag="K8M77_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HBI7086297.1"
                     /translation="MISKREDYISWDEYFMGVALISAKRSKDPNTQVGACIVDNDNKI
                     VGIGYNGFPKGCSDDDLPWGNKGDFLETKYPYACHAELNAILNSTGKNVKNCRVYATL
                     FPCNECAKAIIQAGITEVIYLSDKYKDTDIVKASKFMFNKANVKYRYLGSKRENLLIS
                     FNPNDV"
BASE COUNT         3336 a         1045 c         1311 g         3202 t
ORIGIN      
        1 gtatataata tactcgtaaa tgattataaa attaaaaaag aataaataaa aaaatatata
       61 aaataataaa taagaaagaa aaatttaaag gaggaaataa aaatgaatga taatcaagta
      121 gtatctttta gagatgaaaa tggaaaaaag attgaatatc tggttttaga acaaaaatta
      181 atttgtggtg cagaatatgt agcaatggca cctaaaaatg ataagactca tgtggaacta
      241 tttaagatta attttgataa agattggaat gagactttag attctgtaga atctgaaaag
      301 gaaataaata tgtttaagca agtatctaat ttgaaatttt aataatttaa aataagtaca
      361 tataaattag aatttgtaac tataaaacat atctatataa atattataat tgttttaggc
      421 tctgttttaa gtaaatctta acttttaaac agagcttatt ttaaaaagat ttatataaag
      481 gaggatatac ctcggatcat tatccagtaa tatgtgaatt agaattaaaa tagcagtata
      541 ttcgtagtat gttgtcttat taatttaata aggtataata taaatggatt aaaaactaat
      601 tgtaaattat aataaagttt attcttagag tttttaattt tttaaatcta aatgaatctt
      661 tttattaaga ttttagattt tgacaaaatt tatagcagca tagtacggag gataaaatga
      721 ttttaaacac aaaaagagat ggacatgttc atagtactta ttgcccacat ggtagtaatg
      781 atagtctaga tatgtacatt gatactgcat taagaaatgg tattacagaa ataactttta
      841 cagaacatat gccactacct atggaagacc cttcaccaag aaaaaacagt gctttaccta
      901 tggaaagatt agaggagtac ataaaaggaa taactgagtt aaaagaaaag gttaaggata
      961 aaattaaagt taatgtaggt ttagaagtcg attatgtgga agggtatgaa gaagagacta
     1021 aagctatatt aaataagtat ggaaaatatt tagatgatgc aattctttca gttcatatag
     1081 ttaagtttca aggagagtat tatcaaatag gcaatggatc agatatgatt ggtgaattag
     1141 tagagaaaat tggcggaatg gacaagcttt acgatctata ctatgaaact atgttaaagt
     1201 caattaaatg cgatctagga gcctttaaac ctaaaagaat aggacatcca actttagtta
     1261 gaaagtataa agcagatttt cctaatgaaa aatataatat taagctttta gagaatattc
     1321 taaaagaaat gaaaaaagga aattatgaaa ttgatttaaa tacttcaggc cttagaagag
     1381 cgaaatgtgg agaggtacat cctcaaggaa tatttttaga cttatgttta aagtataata
     1441 tacctatggt attaggatct gattctcata gttcaaaata tgttggagta gagtttgaga
     1501 aggttgcaaa tatgattttc ccagattcag aagcaatata agaaactata acttatataa
     1561 atttattaga aagatttgta tgagaagaga ttttaaaata attatctata aagttttaat
     1621 ctatagataa taaaaaagca cgaggaatta atccttgtgc ttttttatta tttaccgtat
     1681 ataatttttt tagtaacatc attgtagaac attgtgtttg gataatcatt ttcaatttct
     1741 tgcgcatatt ttttagattc agcactgtta ccttctttat tatataaaat agctaagtta
     1801 tataaacatt gaggtgtgta cataaaatct tcagcatctg gctttgcctt ataatccttt
     1861 aaatactctt ggaaatactt gattgcctct gtatttttat ctaagttttc ataagaagtt
     1921 cccatgaaat aaattaagtg aggtctcaag taagaatcag atgaaaaggc ataggctttt
     1981 tcaaagtcct taagagcatc ttcatatttt ttttctttaa ataaatctga tccttttaag
     2041 tataaatcct taactccatc agttttcatt aatttttctg ctttattata agcatctaag
     2101 gcatcttgag gcactttatc cttaggagta gaagttaata atgtataaag agaatttata
     2161 tcattattac taatagcttt atttaattca tcagcagtaa tagtcttagt ttcttttttt
     2221 gaatcttcat ttgttttatt gtcagttgaa ttttcagtct tgttagaatt attattagct
     2281 tcttcacttg ctgttatttg atttttacta gcatctgtag tttttatatt ttttgttact
     2341 ccataagcaa gtaataaaac tatagcacct gaaacagcta cagctatata cttaactggt
     2401 ttctttttgt tagatttttt agcgttagat ttctcgtttt taaaattagg agtactaata
     2461 actttaggtt catcatcttt aggtttatct ttagattttt ttatttcatc ttttttatgg
     2521 aaatctacct ttgttacatt atcaagatga ttactttcat tctttgaagt ttctttaatt
     2581 ttataatcta atggttttat atcatcaatc ttaacatctt ctctaggttt ttctttattt
     2641 atcttaataa ctggagcttg ttcaaccttt ttgttttctt tctctttttc agtatcatta
     2701 gataatggct taatttcagg ttctctcttt tctaattgtt tagcatattc tttttgagct
     2761 tcttcttgaa gtctagcctt aatttcatcc ttcatagttt cactgaatat agtactcatt
     2821 gctaattttt tttcatctaa attattaaat ttatcaagtt ttgttctagc ttcttcattg
     2881 ttgttatact cagcatttat tttccaagaa acaatagcat cgcttattct gttaagcttt
     2941 tcaagtgctc tactttcaag tgtaagagct tcctcgttat tgtagtcctt atcaagtatt
     3001 ttttcacata tatctaatgc ttcttcataa tttccatttt catattgagc taacgctttt
     3061 ttaagttttt tatcatattt actcatagct ttatagctcc tccttttttt ataattttta
     3121 tcaaacatat ttcgataaaa taaataagta tctgtctaaa ttctaattat acattatata
     3181 tataaaagtt tacaatatat ttttggcaaa actatgtcaa tgttcttatt ttttttacaa
     3241 aataagattt tattatgtaa aaaagaacta taaacatttt aatattacta aattaaaaag
     3301 tcattataca cagcatctga aggcattctc caatcacctc taggagaaag tgatatagaa
     3361 ccaacctttg gtccatctgg aagagcagaa cgcttaaatt gttgagtaaa gaatcttctt
     3421 ataaacttat ctagccattt ttttagagtt tcttcatcat aatcattttt aaatgcttcc
     3481 ttagctaaga atagtattct ttcaggactt gatccgtgtt ttataaaatg atataagaag
     3541 aaatcatgaa gttcataagg gccaacaatg tcttctgttt tctgagctat tttaccttct
     3601 ttatcctttg gtaataattc tggacttaca ggagtatcta aaatatctaa taaagcttcc
     3661 ttagcatcat ttgaaatttc atgattagca aaataattaa ctaaaaatct aactaaagtt
     3721 ttaggaatag aacagtttac tgagtacatt gacatatgat ctccattata agtacaccat
     3781 cctaaagcta gttcagaaag atctccagta cctattaata atccaccttc cttattagct
     3841 aaatccataa gtatttgagt tctttctctt gcttgaacat tttcatatgt tacatcatga
     3901 atatctttat catggcctat atcttcaaaa tgttgaagag cggcatttac tatatttatt
     3961 tctctaaaat cacagtgaag ttccttgcat aaagttactg cattattgta tgttctatct
     4021 gtggttccaa aaccaggcat agttatagtt attatatttt ctcttggtat atttagcata
     4081 tcaaaagttt ttaccacaac taataaggca agagtagaat ctaatcctcc tgaaatacca
     4141 attactgctt tcttaaggtt tgtatgctct aatcttttag caagggcagc agcttgtata
     4201 tttaatattt cttcgcagcg ttcttccata gcatactgag attttggtaa aaaaggatgt
     4261 ttatctattt ctctatcaaa gtttttaatc tctgtatttt ctaaattaaa aggaataaat
     4321 ctaggttcat tattttcgtt aaaagttgaa tttctaaaac ttatattttt tattctttct
     4381 gcatctaatt taaataaatc aactatgcca gttaataaat cattttctct tttgaatcta
     4441 ttattttctt ttataatagt tccattttca gctattatca tgtgaccact aaataataaa
     4501 tctgtagttg attcatgaac tcctgctgaa gaataaatat aagaacacat tgttctagca
     4561 ctttggtttt gaactaatga ttttctataa gaactttttg atacaagttc attagaagct
     4621 gaaagattac aaataaggtt agcacccatt aaagctaaat tagagctagg tggtatagta
     4681 acccaaagat cttcacaaat ttcaaaacct acttttaaat ttttaaatga gaatattaaa
     4741 tcagttccaa aaggtacctc cttttgaaaa tctaaatcaa catttaatcc ttttatatta
     4801 aaaccttctg taaaccatct tttttcataa aactcagagt agtttggtat atagcttttt
     4861 ggcactatac ctaaaagttt tccatttaat attatatatg cgcaattata taaggaatat
     4921 ctatataaaa aaggagcacc aacagctatt aaaatatctt ttcctttaga aaattcaact
     4981 agatttttta ttgctaaatt acaattttca attaactttt cgttggtaaa taaatcacca
     5041 caagagtatg ctgatatact tagctcagga aaaactaaaa ccttaacctc ttcttctata
     5101 gcatttaaaa tcatagtttc tatctcttta agattatatt ttgtatcgct tatttttaat
     5161 ttaggtgaag ctgcacctac ttttatgaaa tccatagcat catctccagt atatttttat
     5221 ttttagtatt tctatagtaa taaaagttta tcctttaagt aaaactagat ttttaaataa
     5281 tattttctta tagtagtaac ttttactaaa caaaactaca tattaaatat taatgtgaat
     5341 ttataagaaa atcaacagga gataaatagt attagttacc ttttattaag tttaaaaata
     5401 ttatttaaaa aagtttaaga tagtacttga aataataatg tataaactgt ataataaagt
     5461 aaatattaat aaaaagttaa gattaataaa aaaaggaggg ggcaaactat gttatggctt
     5521 tgggtagtag ttattctagc gggaataata atagactatt taagttctga tgttctattt
     5581 gttggatttt cctttggggc tttaggtggt ttagtattag catttctaga tgtaaatgta
     5641 atattacaat tcatactatt ctccataata acaattttat ttttcttcta tgtttatcct
     5701 aaaattaaaa ggaagataaa agcagataat ctaggaacaa aaaccatgga acaatcttat
     5761 ataggaagaa aaattatatt agatgaaaat gtagaagatt catcattgat taaatttgag
     5821 gggatttatt ggacatttaa aagtgttagt ggagcattaa agaaaggtac agaagtcgaa
     5881 attataggaa tagaagggaa taaaattcta ataaaaaaat aaaaactagg aggggttact
     5941 catggtagct actgtaatta ttataatctt actattaata gtggtatttg cagcaatttc
     6001 atcaatcaag gttgtaaata caggatatgt ttatgtatta gaacgttttg gacaatttag
     6061 taaaatttta gagccaggtt ggcatttagt tattcctttt gctgactttg taagaaagaa
     6121 aatttcaaca aagcaacaaa ttctagatat acctccacaa tatgtaataa caaaagataa
     6181 cgtaaaaatt gaaattgata atgttatatt ctataaagta ttaaatgcta aagatgctgt
     6241 ttataatata gaagatttta aatcaggtat agtttactca acaataacaa acatgagaaa
     6301 catagtaggt aatatgtctt tagatgaagt tttatcaggt agagataaaa taaacttaga
     6361 gcttttaaca ataatagatt ctataacaga tgcttatggt ataaagatac tttctgtaga
     6421 aattaaaaat ataattcctc cagctgaaat ccaagatgcc atggaaaaac aaatgaaggc
     6481 agaaagagat aagagagcta ctatattaca agctgaaggt ttaaaacaaa gtgaaatagc
     6541 aagagcggaa gctgaaaagc aagctaaaat tttaagagct gaggctgaaa aagaagctaa
     6601 cattagacat gctgaaggtt taaaagaatc tcaattactt gaagcagaag gtaaagctag
     6661 agctattgaa gaggtttcaa aggcagaagc tgcagctatt gaaagaataa atacttcaat
     6721 aattgaatct ggaactaacg aagtagtaat tgctttaaaa caagttgaag cattaaaaga
     6781 aatggcagct aacccagcta acaagcttat tttacctaat gaagcagttt cttctttagg
     6841 aagtatagca gctattgctg atattttaaa agaaaataaa gagaataaat aattattatt
     6901 aggaatatac tatttttgag tctttaaaaa ggcttacgta ggcattaatt tttccctttg
     6961 ctttaatgag taaagggatt tttttattta ggcataaatt agtaatttat atattcataa
     7021 aaaaatgtta tcatataaaa tgtaatttca atgtagaaag aggattttaa aatgagtaat
     7081 tatttttggt gcgatgtaaa tttaaagaat atagagaata acataaatgt aataagagat
     7141 ttaatacaaa ataaaaagct gattgcagtt ataaagggag atgcttatgg attaggcagt
     7201 aaaaaaattg ctgaatttat agaagataaa gttgacataa tagcagtagg taatatttat
     7261 gagagtttat gcttagataa tattaataag gatatactta tcttatcacc tttatgtaca
     7321 aaagatgatt ttaaagatga aagagataat ttaatattaa ctttagataa tgaggaaata
     7381 ctaaatgaat tagataagga aacaaagaaa agagtacata tatatgtaga tacaggtatg
     7441 aacagaatgg gtataaagcc taacaagtta gattcattta tagaaagagt taaaaatgaa
     7501 tttcctaacc ttgaaataga tggtatctat actcatttac ataatgctaa gtatgttaat
     7561 tataccttaa aacaaataga attatttaaa tctacagttg aaaagtatag agataaagta
     7621 agaataatac actgcatggc gtcaagtgct attataaatg atcaattaag aaaagcatgt
     7681 gattttacta caggggcaag agccggtaat gttctttatg gatatatagg ctttaataaa
     7741 ggaattaaaa aaacttatga atactatgca aaaccagtaa atacctataa agtttcaaag
     7801 ggacaaactg taggatatgg ttgtcttttt aaggcagata aggatatgga aataggaata
     7861 attgaatgtg gtaatgttca tggcttaggc ataagtagag aaattaaaaa taatgttttc
     7921 tatgatatat ttagaatgat ttatagaaga tttaaagaga gaagtgtaat atttaataac
     7981 aatgtacctg taaaaatatt aggaaaagta aatatgaata taactatcat ctctatggaa
     8041 aactgctcta aagattcagt tttaaaagtt gatatttcac ctataatatc agattcaagc
     8101 atagaaaaaa gatatattta ttagaagcga aaatttatat ttttaagaaa cataaggcta
     8161 aataaatcta gtcttatgtt tcttaatttt ttataatatt tgaacagtta aaataccgcc
     8221 tagttatgtc ataaaagtag caataaaatt ttaaaaaata ataatgaaat tttttaaaac
     8281 ccttgaaaaa aaataaaaaa actttaaaat aagtgttaag tttctattta ggaggttaat
     8341 atgattagca aaagagaaga ttatatttca tgggatgaat attttatggg agttgcatta
     8401 attagtgcta aaagaagtaa ggatccaaac actcaagttg gagcttgtat agttgataat
     8461 gataataaaa tagtaggtat tggatacaat ggttttccta aaggatgttc agatgatgac
     8521 ctaccttggg gaaataaggg ggatttttta gaaacaaaat atccatatgc atgtcacgct
     8581 gaacttaatg ctattttaaa tagtacaggt aagaatgtta aaaactgcag agtatatgct
     8641 actttgtttc cttgtaatga atgcgcaaag gcaataattc aagctgggat aactgaagta
     8701 atataccttt cagataagta taaagatacc gatattgtta aagcctctaa atttatgttt
     8761 aataaagcaa atgtaaaata taggtattta ggaagcaaga gagaaaattt attaatatca
     8821 tttaatccaa atgatgttta aaataaataa accccgtaga taaaactatg gggtttattt
     8881 taagataaat ttat
//
LOCUS       DAEVGI010000097         8821 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEVGI010000097 DAEVGI010000000
VERSION     DAEVGI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.097"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            115..1119
                     /locus_tag="K8M77_001808"
     CDS             115..1119
                     /locus_tag="K8M77_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7086298.1"
                     /translation="MKKFLKVVGVILLSLIIMIGALAYYFRGQIGFYINIGKEYLEFK
                     ENPPSIEDLKNIKMSEDMDFKDVVYKDTNGKAQTLDIYGPEKKLRHGSPVILYVHGGS
                     WVYGNKEIPDLIAPLLDSFRKEGYTIISVGYELATDKVDFKKQASDVKDAIRWVYKNK
                     ETYRFNTDEIGVIGASAGAHLSLLATYSGEDEFVDDKELSNYPSKVKYVVDFFGPTEL
                     SALDMTMASWDLNNSIEKTEKQLRGEESITEYMDKYSPINYVKPNLPKTLIVHGRQDK
                     LVPYSNSMNLYNKSRDMGNDIQILTLENSGHDFSQVDVNEVFELGFKVLNFILFNTKF
                     "
     gene            1278..2843
                     /locus_tag="K8M77_001809"
     CDS             1278..2843
                     /locus_tag="K8M77_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI7086299.1"
                     /translation="MDKRLLKVLKYDYAVEMDKKRNLKAIRILYDMNEKEEYHIDEQT
                     WHDLDLDKVYSKLDRNYSSLGEAALYSMLRNPLNDEKKLNNRREEIEYFKENEEVRFN
                     IMGIFFELGRDKKNSLLDMINEKLIESNKFKYYFYTITGKILPLILILTAIFVSISAM
                     LGLMAVTFVNIYINSKERDRIKANGLMYLRRVIKASKQVVKINDKNLDDFNIKIKENL
                     KDLKSIDRNTRMISFINMWGGLFEFMSVLFLLEETAYYKIADSIEKNKDSILSLYKTL
                     GEMEAIISIGSYEEEKKNKITKPRFIRETTLEIKNGIHPIIENPVANSINMSKRGIVL
                     TGTNMSGKSTFLRMLGVNMLFAQAFNFVLAEKYEGPIFNIVTSISPNDDLSVGKSFYM
                     AEAESILRIIRALDKELPVFCAIDEIFRGTNPIERISASAEILTYINNKNSISIVATH
                     DRELVDILKESYEFYYFSENVDSKNGLSFDYKLKRGVSKTRNAIKLLEYIGYPKDIIN
                     KSYRRSEKLEGFI"
     gene            2858..3544
                     /locus_tag="K8M77_001810"
     CDS             2858..3544
                     /locus_tag="K8M77_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBI7086300.1"
                     /translation="MFKPVKNMKVYEQVVDQIKEMVRVGQIKKGDKLPTERVMAEELQ
                     VSRTSIREAMRALEVVGLIESRQGAGNYIREEFDDVLLEPLSIVFMLQNGTNKDIFEL
                     REVLELSTIFLSVMRISDEELKKLGGLVERFKTSRDEEENVKIDSEFHSIIVKGANNV
                     LITNLLEGVSELVDKFISEGRRAILSDERNRGKLLDFHEKIYIAIKNRDAYSAYKHMQ
                     EHFQLIKKNI"
     gene            3778..5290
                     /locus_tag="K8M77_001811"
                     /pseudo
     CDS             3778..5290
                     /locus_tag="K8M77_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458066.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            5385..6173
                     /locus_tag="K8M77_001812"
     CDS             5385..6173
                     /locus_tag="K8M77_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBI7086301.1"
                     /translation="MKIMVCIKQVPGTSKVEVDEKTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEKKGGNIKVISMGPPQAKAVIKEAYSMGADEGTLVSDRKFAGADVLATSYTLS
                     QGVKKCGDFHIILCGKQTTDGDTAQVGPEMAEYLGIPHVANVRRILDINDDFIKVEMD
                     MPNTIEVLEVKYPCLLTVDKDIFQPRLPSYKKKLETDEREINVISLNDFEDKAEKKYG
                     LNGSPTQVERIFPPSNNDDHEIWTGDSSELSERMEDKLRELKFI"
     gene            6186..7379
                     /locus_tag="K8M77_001813"
     CDS             6186..7379
                     /locus_tag="K8M77_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBI7086302.1"
                     /translation="MGRLVVHQDKIEDANSVIKICPFGAMEIVNGNVEINASCRMCKV
                     CVRKGPKGAFEFVEDENKVLVNKDEWKGIAVYVDHVNGEIHPVTLELIGKARELADKI
                     NQKVYCLFMGNGIKDKAGELLHYGVDEVIVYDYKELENFRIEPYTAVFYDFINKIKPC
                     SILVGATTLGRSLAPRVAARCRTGLTADCTILDIKENTDLVQIRPAFGGNIMAQIVTP
                     NSRPQMATVRYKVMSAPERNEECTGEIKYFEIEKDKLKSDIEVKEIKQKPKEHSISDA
                     EVIVVAGRGVKSEKDLEIIKELADLLGGELAVTRPLIENGWADANRQVGLSGRTVRPK
                     LIITCGVSGAIQFVAGMNNADYIFAINKDEKAPIFKVAHYGIVGDIYEIIPKLIEKIK
                     GEGVL"
     gene            7381..8781
                     /locus_tag="K8M77_001814"
     CDS             7381..8781
                     /locus_tag="K8M77_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBI7086303.1"
                     /translation="MSYNKINESGIEFIKGIINDNERVFIGEEISEDYSHDELGSEKH
                     MPDIVVKAMNRDEIVEIMKYANNNNTPVTVRGAGTGLVGAAVPILGGILLDLSGMNKI
                     LELDEDNLTLTVEPGVLIMEIAKFVEEHDLFYPPDPVEKTATIGGNVSTNAGGMRAVK
                     YGVTRDYVRGMEVVLPSGEVLELGGKVVKNSSGYSLKDLIIGSEGTLGIITKIVLKLL
                     PLPKKIISLLVPFNDLESAIETVPKIIKSKNIPTAIEFMQRDLIISAEEFLGKKFPDN
                     SSNAYLILSFDGNSKEEVERYYEKVADICLKEGALDVFISDTEERQESIWSARGAFLE
                     AIKASTSDLDEVDVVVSRNKVAEFVKFTNEVEEKLNVRIRSFGHAGDGNLHIYILKDE
                     LSEGEWHKKLNEAMEILYRKQRELNGQVSGEHGIGLAKRLYLNESLNKATMDIMRGIK
                     LAFDPNNILNPGKVCE"
BASE COUNT         3447 a          898 c         1702 g         2774 t
ORIGIN      
        1 ttttaaatat ataaaaattt aaaatagatg taagtataat tgtgtattta ccctgattta
       61 aggttattat ttaaatgaag tataggaaaa atttaaatta taaggaggga gagaatgaaa
      121 aaatttttaa aagtagtagg agttatatta ctaagtttaa taattatgat tggagcttta
      181 gcttattatt ttagaggaca aataggtttt tatataaaca taggaaaaga gtatttagag
      241 tttaaggaaa atccacctag tattgaggat ttaaagaaca ttaaaatgag tgaggatatg
      301 gattttaagg atgtagttta taaagatact aatggaaaag ctcagacttt agatatttat
      361 ggaccagaaa agaagttaag acatgggtca ccagtaattc tttatgttca tggaggaagt
      421 tgggtttatg gaaacaaaga aataccagat cttatagcac ctcttttaga ttcctttaga
      481 aaagaaggat ataccataat aagtgtaggg tatgaacttg ctactgataa ggttgatttt
      541 aagaaacaag cttcagatgt taaggatgcc ataagatggg tttataaaaa taaagaaact
      601 tatagattta acactgatga aataggagtt ataggggctt ctgctggggc acatttatcc
      661 ttgttagcca catatagtgg ggaagatgaa tttgttgatg ataaggaact ctctaactat
      721 ccatcaaagg ttaaatatgt tgtagatttc tttggaccaa ctgaacttag tgccttagac
      781 atgactatgg cttcatggga tttaaataat tcaatagaga aaactgaaaa gcaattaaga
      841 ggggaagaaa gtataacaga atacatggat aaatatagtc caataaatta tgttaagcct
      901 aacttaccaa agactttaat agttcatgga cgtcaagata aattggttcc atacagtaat
      961 tcaatgaact tatataataa gagcagggat atgggtaacg atattcaaat acttacctta
     1021 gaaaatagtg gccatgattt ttctcaagtt gatgtaaatg aagtatttga actaggtttc
     1081 aaagttttaa attttatact ttttaacact aagttttaat agaaagctaa atttatgtat
     1141 attttaataa ctacttagca tataagattt ttaaaattaa agtttataaa ttatatcata
     1201 attggttcaa atttcacttt tattgtaaaa gaagatataa taaaaccatc aaattttttt
     1261 agggtaagga gttttatatg gataagaggt tgttaaaagt attaaagtat gattatgcag
     1321 tagaaatgga taaaaagagg aacttaaaag ccataagaat actatatgat atgaacgaaa
     1381 aagaagaata tcatatagat gaacaaacat ggcatgattt agatttagat aaggtttatt
     1441 caaaattaga tagaaattat agtagtttag gagaagctgc actttattca atgcttagaa
     1501 atccactaaa tgatgaaaaa aagttaaaca atagaagaga agaaatagag tattttaaag
     1561 aaaacgaaga agtaagattt aatattatgg ggatattttt tgagttaggg agggataaaa
     1621 agaatagctt gttagatatg attaatgaaa agctaataga atctaataag tttaaatatt
     1681 atttttatac aattacagga aagatattac ctttaatatt aatacttact gcaatctttg
     1741 taagcataag tgcaatgctt ggcttaatgg ctgtaacctt cgttaatatt tatattaata
     1801 gtaaggaaag agaccgtatt aaggctaatg ggcttatgta tttaaggaga gtaataaagg
     1861 catctaagca ggtagttaaa attaatgata aaaatttaga tgattttaat ataaagataa
     1921 aagaaaactt aaaagattta aaatcaatag atagaaatac tagaatgatt agctttatta
     1981 atatgtgggg tgggttattc gaatttatgt cagtattatt tttattagaa gaaactgcat
     2041 attataagat tgctgattca atagagaaaa ataaagattc aattttaagc ctttataaaa
     2101 cattaggtga aatggaagca ataatatcta ttggaagtta tgaagaagaa aaaaagaata
     2161 aaataactaa acctagattc ataagagaaa caacattaga aataaaaaat ggaatacacc
     2221 caattataga aaatccagta gctaactcca taaacatgag taagagagga attgttctta
     2281 ctggaactaa tatgtcaggt aaatcaactt ttttaaggat gttaggagta aatatgcttt
     2341 ttgctcaagc ctttaatttt gttttagcag aaaaatatga ggggccaata tttaatatag
     2401 ttacttcaat tagtccaaat gatgatttaa gtgttggtaa aagtttttac atggctgaag
     2461 cagaatcaat tttaagaata ataagagcct tagataagga gttaccagta ttttgtgcaa
     2521 ttgatgagat ttttagaggg acaaatccaa tagagagaat atcagcttca gcagaaattc
     2581 ttacttatat caataataaa aacagtattt ctatagttgc aactcatgat agagaattag
     2641 ttgacatatt aaaagaaagt tatgaatttt attattttag tgaaaatgtt gatagtaaaa
     2701 atggattaag ctttgattat aagctaaaaa ggggagtatc taaaactaga aatgctataa
     2761 aattactaga atatataggt tatccaaaag atataataaa caagtcatat agaagatcag
     2821 aaaagctaga aggctttatt tagaaaggat tgttaggatg tttaaaccag ttaaaaacat
     2881 gaaagtttat gagcaggttg ttgatcaaat aaaagaaatg gttagagtag gacagataaa
     2941 aaaaggagat aagcttccta cagagagagt tatggcagaa gaacttcaag taagtagaac
     3001 ttctataaga gaagccatga gagctttaga ggttgtagga ttaatagaaa gtagacaagg
     3061 agcaggtaat tatataaggg aagagtttga tgatgtatta ttagaaccgc tttcaatagt
     3121 atttatgctt caaaatggaa caaataaaga tatatttgaa ttaagagaag ttttagaatt
     3181 gtctactata tttttatctg taatgagaat atctgatgaa gaattaaaaa agcttggtgg
     3241 acttgtggag aggtttaaga catctaggga tgaagaagag aatgttaaaa ttgatagcga
     3301 atttcatagc ataattgtta aaggtgcaaa taatgtactt ataactaatt tattagaagg
     3361 tgtatctgag ttagttgata agtttataag tgaaggaaga agagccattt taagtgatga
     3421 gagaaataga ggaaagctat tagattttca tgaaaaaata tatatagcta taaaaaatag
     3481 agatgcttac tctgcatata aacatatgca agagcatttt caattaataa agaaaaatat
     3541 ttagtaatag aaactttatc taaaattaaa gtgaatttta acctattata agcatatata
     3601 ataaatgtaa aaattgacta aaatatctaa aaggtataag gttaaattag ggtataaaaa
     3661 gagactttta gtaataaata tcaatctagc atattaaaat aatatgctag attttataaa
     3721 gcaaaattgg tcataccaaa attatagtat aaaagtttat tatgaggggt gaaagaattg
     3781 aatacgtatc ttatgttttt tcttgctctt atacctataa ataggtttag gagttcttaa
     3841 aattccagct cataaaatat gtcccatagc tttaataatt accattttac tttccatatt
     3901 tatttggaag atgcctacta tagactcctt aacagcagcc ttagaaggta ttgtcctagg
     3961 aatatggcca ataatgatag ttattatagc ggcagtattt acttataatt tagtagttca
     4021 tacaaaggcc atggaagtaa taaaatcaat gttaataagt gttagtgatg ataaaagaat
     4081 tttagttctt atactagcat gggaatttgg aggcttttta gaagctgtgg caggatttgg
     4141 aacagcagtt gctattccag ttggaataat ggcagcctta gaatttaatc ctttttttgc
     4201 cgctgttata tgtttagtgg ctaatacaac tcccacagcc tttggagcaa ttggtcttcc
     4261 tgttataacc ttagctaata tatcaggaat tcctatagaa tccttaagtt attatgtagt
     4321 actacaatta tttatctttg ttgtattaat tccatttgcc ttggtagctt taacttcaaa
     4381 aagtttaaag gggataaagg gagtctttgg tataacttta gcctctggtc tttcctttgg
     4441 catagttcaa gtccttgtag caaaattttt aggccctgaa ttaccagcag ttttaggatc
     4501 aatagtttct ttattagtta caatttttat agcaaaaaaa ttctataaac ataatgaaga
     4561 aacaaaaaga gaagaaaagt ttgaattaaa aagtgtctta gtagcatttt caccatttat
     4621 attaatatta gtcataataa taggaattag tcctttattt ccaggcttat ataataaatt
     4681 aagcgaagta aggacattag ttaatatata tactggtgtg ggagctaaac catatacttt
     4741 tgtatggtta acaactcctg gaatattaat attaatagct tcttttattg gagggtttat
     4801 acaaaaagcc tcttttaaag aaatggtaga aattttatta aagacaatta aacaaatgag
     4861 taaatctacc ataaccatac ttgcaataat agctctagct aaggtaatgg gctatagtgg
     4921 aatggttgca tctatagcaa cagtattagt tatgataaca ggttcatttt atgcttttat
     4981 agcacctttt attggagctt taggaacctt tgtaacagga agtgatacat ctgccaatgt
     5041 attatttggt tctcttcaag ctgaggcagc aaattcttta ggaataagcc cagcatggct
     5101 aggggcagca aatacttgtg gggcaactgc aggtaaaatg atatctcctc aaagcatagc
     5161 tgtggccatt gcggcaacag gactagctgg taaagaagga aagatattaa attcaacatt
     5221 aaaagtatgt atagtatatg taattttatt aggtttaata agttatttag gaagcttaat
     5281 ttttgtgtaa ttcttgaaaa cgtttattga tgtttattaa ttaaagtctc aattattaaa
     5341 gtattaaaaa ttaaaggatt tataaaggga atggagtgat ttttatgaag attatggttt
     5401 gtattaaaca ggttccagga acttcgaagg ttgaggttga tgaaaaaaca ggggttttaa
     5461 agagagatgg agtagattct aaaatgaacc cttatgattt atatgcttta gaaactgcat
     5521 tgaggataaa agaaaagaaa ggtggaaata taaaggttat aagtatgggg ccaccacaag
     5581 ctaaggcagt aataaaagag gcttattcta tgggggctga tgaaggaacc ttagtttcag
     5641 atagaaagtt tgctggggct gatgttttag caacttcata tacattatca caaggagtta
     5701 aaaaatgtgg ggattttcat attattcttt gtggaaaaca aactacagat ggagatacag
     5761 cacaggttgg acctgaaatg gctgaatatt taggaattcc tcatgttgct aatgttagaa
     5821 gaatattaga tattaatgat gattttatta aagttgaaat ggatatgcct aatacaatag
     5881 aggtactaga agtaaaatat ccttgtcttt taactgtaga taaagatatt tttcaaccta
     5941 gattaccttc ctataaaaag aaacttgaaa cagatgaaag agaaataaat gtaatatctt
     6001 taaatgattt tgaagataaa gctgagaaaa aatatggttt aaatgggtca ccaactcaag
     6061 ttgaaagaat atttccacca agcaataatg atgaccatga gatttggaca ggagattcat
     6121 ccgaattaag tgaaagaatg gaagataaat taagagagct taagtttata tagggggtaa
     6181 gagttatggg aagattagtt gttcatcaag ataaaataga ggatgcaaat agtgtaataa
     6241 aaatttgtcc ctttggagct atggaaatag ttaatgggaa tgttgaaata aatgcaagtt
     6301 gtagaatgtg taaggtatgt gttagaaaag gacctaaagg agcctttgag tttgttgaag
     6361 atgagaataa ggtcttagta aataaggatg aatggaaagg tatagccgtt tatgttgacc
     6421 atgtaaatgg agaaattcat ccagttacct tagaactaat aggaaaagct agagaattag
     6481 ctgataagat taatcaaaaa gtttattgtt tatttatggg gaatggaata aaagataagg
     6541 ctggagagct tttacattat ggagtagacg aagttattgt atatgattat aaagagcttg
     6601 aaaactttag aattgagcca tatacagctg tcttttatga ctttataaac aagattaaac
     6661 cttgttcaat tttagtagga gccactacat taggtagatc attagcccca agagtagccg
     6721 ctagatgtag aacagggctt acagcggatt gtactatact tgatataaaa gaaaatacag
     6781 atttagtcca aataagacca gcttttggtg gtaatattat ggcgcaaata gtaactccaa
     6841 attcaaggcc tcaaatggca actgttagat ataaagttat gtctgctcct gaaagaaatg
     6901 aggaatgtac tggagaaatt aagtactttg aaatagaaaa ggataaatta aaatcagata
     6961 tagaagttaa agaaattaaa caaaaaccta aagaacatag catatcagat gctgaggtaa
     7021 tagtagtagc tggtagaggt gttaaatcag aaaaagattt agaaataata aaagaacttg
     7081 cagatttatt aggtggagag cttgcagtta caagacctct tatagaaaat ggatgggctg
     7141 atgctaatag acaagttgga ttaagtggaa gaacagtaag accaaaactt ataataactt
     7201 gtggagtttc tggagctatt caatttgtag caggaatgaa caatgcagac tatatatttg
     7261 ccataaataa agatgaaaaa gcacctatat tcaaggttgc tcactatgga atagttggtg
     7321 atatttatga aattattcct aaacttattg aaaaaataaa aggggaaggg gtgctttaat
     7381 atgagttata acaaaataaa tgaaagtgga attgaattta taaaaggaat aataaatgat
     7441 aatgaaagag tatttattgg agaagaaata agtgaagact attctcatga tgagttaggt
     7501 tcagaaaagc atatgcctga tattgttgtt aaggctatga atagagatga aattgtagaa
     7561 ataatgaaat atgcaaataa taacaatact cctgtaactg taagaggagc tggtactgga
     7621 cttgttggag cagcagttcc aatactagga ggaattttat tagatttaag tggaatgaat
     7681 aaaatattag agttagatga agataactta actcttacag ttgaacctgg agttcttatt
     7741 atggaaatag ctaaatttgt tgaggaacat gatttatttt atccaccaga tccagtagaa
     7801 aaaacagcaa ccataggtgg taatgttagt acaaatgcag gaggaatgag agctgttaaa
     7861 tatggagtaa caagagatta tgtaagagga atggaagtag ttcttcctag tggagaagta
     7921 ttagaacttg gtggaaaagt tgttaaaaat agttcaggat atagcttaaa agatttaatt
     7981 attggatcag aaggaacatt aggaataata acaaaaatag tattaaaact attaccactt
     8041 ccaaagaaga taataagtct tttagttcca tttaatgatt tagaaagtgc catagaaaca
     8101 gtgcctaaaa taataaaatc taagaatatt cctacagcta tagaatttat gcaaagagat
     8161 ttaataattt cagcagagga attcttagga aagaaatttc ctgataattc atcaaatgct
     8221 tatttaatat taagctttga tggaaactca aaggaagagg ttgaaagata ttatgaaaaa
     8281 gtagctgaca tatgtttaaa agaaggagct ttagatgtat ttatatcaga tacagaagaa
     8341 agacaagaat caatatggtc agctagggga gctttcttag aagccataaa ggcatcaaca
     8401 agtgatttag atgaggttga tgttgttgta tcaagaaata aggtagcaga gtttgtaaaa
     8461 ttcactaatg aagtagagga aaaacttaat gttagaataa gaagttttgg acatgctggt
     8521 gatggaaact tacacattta tatattaaaa gatgaattat cagagggtga atggcataaa
     8581 aaacttaatg aagctatgga aatactctat agaaaacaaa gagaacttaa tggacaagtt
     8641 tctggagaac atggaatagg cttagctaag agattatatt taaatgaatc cttaaataag
     8701 gccacaatgg atattatgag aggaataaaa ttagcttttg atccaaataa cattttaaat
     8761 ccgggtaagg tttgtgaata aaataagttg aaaatttaat tagcatataa aatttaacaa
     8821 t
//
LOCUS       DAEVGI010000098         8818 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEVGI010000098 DAEVGI010000000
VERSION     DAEVGI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.098"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(745..1170)
                     /locus_tag="K8M77_001815"
     CDS             complement(745..1170)
                     /locus_tag="K8M77_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBI7086304.1"
                     /translation="MNIKNLANQLVKKYGLRDPLEIVLAKGIILLEEDLGEVYGYSHT
                     YKRQSFIHLNKNCSLKTKRLVCAHELGYLLIHPKEEIHVVTDKSLEPTDDFERYAKIF
                     AAHLLISDEMVKEYEGKNAEDMANSLNLPLDLVLIKLNS"
     gene            complement(1305..1847)
                     /locus_tag="K8M77_001816"
     CDS             complement(1305..1847)
                     /locus_tag="K8M77_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086305.1"
                     /translation="MFIGYRLQKLRKKRKLTQKALAEMTGISRSYLSDIEHNRYNPSF
                     DTIEALATALKLDLKSFFDDTLLEEDYYLKPLNEELEDERFEEIEVLSENEIVEKQII
                     NNDLNITYKTGSLSKKEILNIKADLEKTLNNLDNFEKNIAFEGFLLDSETRKAFKESL
                     EHSMKMAKLIAKNKFSSKAN"
     gene            2803..4731
                     /locus_tag="K8M77_001817"
     CDS             2803..4731
                     /locus_tag="K8M77_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HBI7086306.1"
                     /translation="MENYKAVFTISEVMKYLNIHDKKVVAFDIECAPKNEWFRLNEKK
                     AALDPNKGHIVGISLSVKEGTGIYIPLRHRNGKNVEDVHRMIAWLKGFVFHNESLIKV
                     AHNIAFESSFLYEYGIVIKEPVFDTMLGSMLTFKSFREFRTLRDSGLKLLAKEIFNED
                     LPSFSHVVNGTHFDNLHSEHKETVRYACADSDYALRLYNWCLEWFETYIPEHRLVCER
                     VESPAAIYTGIMKKNGITLNKELLQEFNRRAEEELEFCKREILSFANREVNLGSNLQT
                     QDFKDFLFKDLRLPVLKICEKGEPSLDDESLILLEEWCLRNFKKGTYLIECIKRHKRV
                     SKLKSTYIEALGKLLNSETGKIHTDFFSMGTETGRFSSRNPNLQNLPRKDNDHLGIRK
                     LFIPSPNHVFLDFDFSQIELRVGAYYCKDRKMINAFMKDFDIHGITTSIIYNIPLEEA
                     TDRNHEDFKKRRTIAKNCNFGVFFGLFPKGLQKTLMYKGGINISEKDCERIIRNIKDG
                     YPRLSTWQKDTVKVAAYRGYTRSRTGRRRYLKDIKSKDWNKRTVSERCALNTPIQGTA
                     ADIIKIAMARILRELPERDYIKPLLQIHDELLFEVREDKVNEAIAFIKDCMERTPFKD
                     FNIPLKVEYSVGYNFDEV"
     gene            4857..7760
                     /locus_tag="K8M77_001818"
     CDS             4857..7760
                     /locus_tag="K8M77_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI7086307.1"
                     /translation="MNKEEFGKCNLSEARRFLECFCKVECSEGTSYSGKSTELKEFDG
                     KSSELKEYEGTSFDSKGNELKGSENSCGRINIRIFSDKKGTGFKGKNLSFNIKDFQSK
                     SKVLMAHNEANRGIFFVVNSGGNSDSKINKINAQFFECDTLSLEEQLENISKFPLEPS
                     IIVQTKKSLHVYFLIKNGKVEKFRDIQKKLAKHFNGDGSCINESRVMRVPGFYHCKEE
                     PVRVKCIKFNPNLFYTQEDLERELSYSESEFIVNDDNYIRLESYISNYHSDLQESTLL
                     NKENISEDYRKSISEKKSEVRNNKSKEQVLYESEENLHSMSEDNISKNNQLNIENTVD
                     EELKSENGLEVVCFKCDFINHCKKNSKILSEPLWHGMITNLALFKGGTYRIHELSKGY
                     KTYSEKETEEKISNFLKSDAGPMTCETLRDRGYTCPRYGKTCYGKSPASLAFKPLTVK
                     DIRECIKTLKVSEVSNAINVDTALRFIENYMYNIGVALGKSLIEEDLANHLKIKNSKD
                     LISFYREVVRNFKKERGNKAKSKNKSKPKNSGNLPWYEEQEKGLKFLPFVLAKHLSET
                     RDVYYGGESFLIYENGVYNISGEKEAGRIIMDYMLPNYCVMASIRDCREQWDILVSKD
                     FDDFNRNPYLVNVRNGLLDIRDMSFKEHTPSYLSTVQLNVEYNPQVDCPQFKKFLNEV
                     LDCKLIPLVQEIVGYLLTTNTASQKAFVFWGPARTGKSTLLWVVEYLLLGKKNVSNIP
                     WQEIGDKFKTAELLGKLANVFSDLPSKSIDDTGIFKVVTGEDYLMAEKKNKNPFKFKP
                     FARLVFSCNELPRNYVDRTEGFYRRLIIVPFNRQIEKSKIDKSLKYKFQREKEGILNW
                     ALEGLKRLYENNFEFSENELTDGIKKEYKRENNNVISFVEECCELDGLFSCSRIELYE
                     SYKEFCVEAGLKALSQIKFNKELEGNFNITRSRSGKLRSWNGVRIKLEDLIIR"
     gene            8182..8802
                     /locus_tag="K8M77_001819"
     CDS             8182..8802
                     /locus_tag="K8M77_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086308.1"
                     /translation="MKKGIWVTISILSLGVFLALSFFIIAKKEIKDYKNIGIDLTYDE
                     RVKEPIEELLVKFVDFDYSKEEVNCEEIISNKEVAATYNLTFNSTLKYNLESELKMSK
                     VSIKSIVKEPDNEYRVKFHREFEIKFDKNSDTISSGMDDYTAYIVEKNGEFYIDKILN
                     DVDFNQFKNSKSKITKLFKSEEELFNEAMKSEIEARRNYEEYLKNL"
BASE COUNT         3188 a          938 c         1717 g         2975 t
ORIGIN      
        1 tcataattaa attctttgtt tgatattttt aaagaaaata agtttaaatt ataattatca
       61 aattcattat ttgatatcaa atgattaaaa atatttaaat aattttttgg tacaaaactt
      121 tgaatttctt ccattagtat ctccaatctt aaaattgtag ttatattaat ggaataatta
      181 taccataata ttcttttatt tataaatttt atgatattta attatgtttt gtaaaaagaa
      241 tatattaata tctcatcagt aatttgtaaa cttagttatt ataattccat gtgaaaattt
      301 tgcttaatat gagaataata ctttaaattt atattagaat agaagtaata aaatagactt
      361 aacagataga ttattatata tagacattat atggtggtaa ataattgtat taagatttag
      421 tgttttttag tagattagaa gatagattgt tttgaagttt taataagtaa agaaatgaaa
      481 atttttttac attttgtatc attatataga gttaaaaaat agtagacttt accttttgaa
      541 tattagaaaa gatatttcaa attataaaag aacggagtca aaataaagga attttattag
      601 agctttgaga aatctagaga tgttgactca aaattctatg aggattcaaa aaattatgaa
      661 ttaatatatt attagaatta tttttattaa ataccttgtc caaataattg gttttggaca
      721 agggtatact gtttattaaa tatcttaaga attaagttta atcaaaacta aatccaaagg
      781 tagatttaaa gagttagcca tatcttcagc attttttcct tcatactctt ttaccatctc
      841 atctgatata agtaagtgtg ctgcaaagat tttggcatat ctttcaaagt catccgttgg
      901 ttctaaggat ttatcagtta caacatgtat ttcttctttt gggtgaatta gaaggtatcc
      961 taattcatgg gcacatacaa gccttttggt ttttaatgaa caattcttgt ttaagtggat
     1021 gaagctttgc cttttatagg tatgggaata gccatatact tctcctaagt cttcttctaa
     1081 aagtatgatt cccttggcta atacaatttc taatggatcc cttaatccat atttttttac
     1141 taattggttt gctaaatttt ttatattcat atatattttg gattgcacca atgaaactct
     1201 tgattaaatt tatgagtttt gatgcaagtc cttcacctac ctttcaaata ttttataatg
     1261 aaaggatatt ggtgttttaa gcaggtttaa ttgattttaa ttggctaatt tgctttagaa
     1321 gaaaatttgt ttttggcaat tagttttgcc attttcattg aatgttctaa gctttcctta
     1381 aaagcttttc ttgtttcact atctaagagg aacccctcaa aggctatatt cttttcaaaa
     1441 ttatctaagt tatttagagt tttctctaaa tctgctttaa tatttaatat ctcctttttt
     1501 gaaagagagc cagttttata agtaatgttt aaatcattat taattatttg cttttcaaca
     1561 atctcatttt cagaaagcac ctcaatttct tcaaatcttt catcttctag ctcttcattt
     1621 agaggtttta aataataatc ttcttctaag agagtatcat caaaaaatga ttttaaatca
     1681 agctttagag ctgtggctaa tgcctcaata gtatcaaagc tagggttata tctgttatgt
     1741 tctatatctg agagataaga acgagatata cctgtcattt cagctaaagc cttttgagtt
     1801 aattttcttt tctttcgcaa tttttgtagc ctataaccaa taaacataag ttaaattccc
     1861 ccttttgttt taaagtttta ctctgattta gagtagtttt gattttcata tatattaaaa
     1921 tcaacatcca ctaaaaacag aattttattt ttaaattgat tatggtttaa ctaaactttg
     1981 aatttagaat atataaagat atttagacct tattctaagt ttatatattt tgtttggaat
     2041 tttgaaaata ttaaaattta tgtatggtaa cacttgtatt ttaagaaagt agtgttactt
     2101 aagtcactat aagtttaata atttaaggta attgtatttt actaggttaa atgtaaaata
     2161 ttgttataat attattttaa cagggtaatt tcgaacaatc aatagtttct acaaaaaaag
     2221 taaaacaata cagactttgt aaggaaaaag gagcaaatga aagaaatatg aaaataaaga
     2281 agattaattt ctaataaaag gataatttga attttggtat tggaagaaaa aggtattata
     2341 atgccattat aaggattggt aaggagaata ctaggagtat ttgtaatact gtacactaat
     2401 ttgtgaaagt gatacaaatt ttgcagaaat ccttatttgg atattagtgt ttaataagta
     2461 aatattaaaa atctctttta taggttttaa agtaaaggtt ttaattgatt gggaatttgg
     2521 aaaatttatg atttatgtaa agctttaaat gaactagcaa agaagaatct ttttgtataa
     2581 actaaagaat ttaaattaag aatacttaag cttattttga ttatttacta tggataatgc
     2641 ggagagaaac ttatttttaa ggatttaaaa gttaagggtc ataggataaa aatccaatgg
     2701 atatttttaa aatattctta taaaaggttt aaagggtaat agtcagagga taaaaatctt
     2761 aagtgaaata attgaaattt tttatataag gagagaaaag ttatggaaaa ttacaaggct
     2821 gtttttacta ttagtgaggt tatgaagtat ttaaacattc atgataagaa ggttgtggcc
     2881 tttgatattg aatgtgcacc aaagaatgag tggtttagat taaatgagaa gaaggctgct
     2941 ttagatccaa ataagggaca cattgtgggt atatcccttt ctgttaagga gggtactggt
     3001 atttacatcc ctttaaggca tagaaacggg aaaaatgttg aggatgttca tagaatgata
     3061 gcttggctta agggctttgt ctttcacaat gagagtctta taaaggttgc tcacaatata
     3121 gcctttgaat cctccttcct ttatgagtac ggaattgtta ttaaagaacc tgtttttgat
     3181 actatgctag gtagtatgct tacctttaag agttttagag agtttagaac tttaagggat
     3241 agtggtctta aacttcttgc aaaggagata tttaatgagg atttgcctag cttttcccat
     3301 gtggttaatg gaacacactt tgataatttg cattctgagc ataaggaaac tgtaaggtat
     3361 gcctgtgcag atagtgatta tgccttaagg ctttataact ggtgcttaga gtggtttgag
     3421 acttatatac cagagcatag gcttgtatgt gaaagggttg aaagtccagc agctatttat
     3481 actggtatta tgaaaaagaa tggtattacc cttaataagg agcttttaca ggagtttaac
     3541 agaagagctg aggaagagct tgaattttgt aagagggaga ttttatcctt tgcaaataga
     3601 gaagttaacc ttgggagtaa tcttcaaact caggatttta aggattttct ctttaaggat
     3661 ttgaggctac cagttcttaa gatatgtgaa aaaggcgagc cttccttaga tgatgagagc
     3721 ttgattctcc ttgaagagtg gtgtcttaga aattttaaga aggggactta tcttatagaa
     3781 tgcataaaga ggcataagag ggttagtaag cttaaatcta cttatataga ggctttgggg
     3841 aaacttttaa actcagaaac tgggaagatt catacagatt ttttctctat gggaactgag
     3901 actgggagat tttctagcag gaatccaaat cttcaaaacc ttcctagaaa ggataatgac
     3961 catttaggca ttagaaaact ttttattcct tcacctaatc atgtgttttt ggattttgac
     4021 ttttctcaga ttgagcttag ggttggggct tattattgca aggatagaaa gatgattaat
     4081 gcttttatga aggattttga tattcatgga ataacaactt ctattatata caatattccc
     4141 ttagaggagg ctacagacag gaatcatgag gattttaaga agagaaggac catagctaaa
     4201 aactgtaact ttggtgtttt ctttggacta tttcctaagg gacttcaaaa aacacttatg
     4261 tataagggtg gaattaatat aagtgaaaag gactgtgaaa ggattataag aaatataaag
     4321 gacggatatc caaggctttc aacttggcaa aaagatactg tgaaggttgc agcgtatagg
     4381 ggatatacaa ggagtagaac tgggagaaga cgttatttaa aggatataaa atctaaggac
     4441 tggaataaga ggactgtatc tgaaaggtgt gccttaaaca ctccaataca gggaacagca
     4501 gcagacataa taaaaatagc catggcaagg atactaaggg aattaccaga aagagactat
     4561 ataaaaccct tgcttcaaat acatgatgaa cttttgtttg aagttaggga agacaaggtt
     4621 aatgaggcca tagccttcat aaaggactgt atggagagaa ctccctttaa ggactttaat
     4681 atacccctaa aggtggagta ttctgtggga tataactttg atgaggttta gttaaaagtt
     4741 tacagttgtt taagctttgg acaagctata aagtaataag aggttattat aagttaggtt
     4801 aaaagtttat ctacgtaaaa gaatctaaaa aagagagatg aaagggtgtt tgaaaattga
     4861 ataaagagga atttggaaag tgcaatttaa gtgaggcaag aaggttttta gaatgttttt
     4921 gtaaggtgga atgttctgag gggacaagct attctggtaa aagcactgag ttaaaagaat
     4981 ttgatgggaa aagtagtgag ttaaaagaat atgaggggac aagctttgat agtaaaggaa
     5041 atgagttaaa aggctctgaa aattcttgtg gaaggattaa tattaggatt ttttctgata
     5101 agaaggggac aggctttaag gggaagaacc taagctttaa tataaaggat tttcagtcta
     5161 aaagcaaggt tttaatggct cataatgagg ctaatagagg catattcttt gtggttaact
     5221 ctggaggaaa cagtgatagt aagataaata aaattaatgc tcagtttttt gagtgtgata
     5281 ctctttcctt agaggaacag ctagagaata taagtaagtt tccactagag cctagcatta
     5341 tagtacaaac taagaagtct ctacatgtat attttctaat aaagaatgga aaggtggaga
     5401 agtttaggga tattcaaaag aaactagcca agcatttcaa tggggatgga agctgcataa
     5461 atgagagcag agtcatgaga gtgccaggtt tctatcattg taaggaagag ccagtaaggg
     5521 taaaatgcat aaagtttaat ccaaacctat tttataccca agaggattta gagagagaac
     5581 ttagttatag cgaaagtgag ttcatcgtaa atgatgataa ttatataaga ttagagagtt
     5641 acatttcaaa ttatcatagc gatttacaag agagtacctt attaaataaa gaaaacattt
     5701 cagaggatta tagaaaaagc atttcagaaa agaaaagtga agttagaaac aacaaatcta
     5761 aagagcaagt tttatatgag agtgaagaaa atttgcatag catgtcagag gataatattt
     5821 caaagaataa tcaacttaac atagaaaata ctgtggatga agaattaaag agtgaaaatg
     5881 gattagaggt tgtgtgtttt aagtgtgatt ttataaatca ctgtaagaag aactcaaaaa
     5941 ttctttctga gcctttatgg catggaatga ttactaacct tgctcttttt aagggtggaa
     6001 cttataggat tcatgaactt agtaaggggt ataagaccta tagtgagaag gaaactgagg
     6061 agaagattag taattttcta aagtcagatg caggtcctat gacttgtgaa acccttagag
     6121 atagggggta cacttgtcca aggtacggta agacttgtta tggcaaaagt ccagcctctt
     6181 tagcttttaa gcctttaact gttaaggata ttagagagtg cataaaaact ttgaaggtta
     6241 gtgaagtttc taatgccata aatgtagata ctgcccttag gttcatagag aattatatgt
     6301 ataacattgg agtagcttta ggtaagagtc ttattgagga ggatttagct aatcatttaa
     6361 agattaagaa ttcaaaggat cttatttcat tttataggga ggttgttagg aattttaaga
     6421 aggaaagggg gaataaggct aagagtaaaa ataagagtaa acctaagaat tcagggaatc
     6481 ttccatggta tgaggagcag gaaaagggac ttaagttctt gccctttgtt ttggcaaagc
     6541 atttaagtga gactagagat gtttactatg gtggggaatc ctttttgata tatgaaaatg
     6601 gagtttataa cattagtgga gagaaggagg ctggaagaat catcatggat tatatgctac
     6661 ctaattattg cgttatggca tctattaggg attgtagaga gcagtgggac atacttgttt
     6721 ctaaggattt tgatgatttt aatagaaatc cttatttggt taatgtaaga aatggacttt
     6781 tagatattag ggatatgagc tttaaggagc atacccctag ttacttaagt acagttcagc
     6841 ttaatgtaga gtataatccg caagttgatt gtccacaatt taaaaaattc ttaaatgaag
     6901 tgcttgattg taagctaata cccttagttc aggagatagt agggtattta cttaccacaa
     6961 atactgcctc tcaaaaggcc tttgtattct ggggaccagc tagaacagga aaaagtacac
     7021 ttttatgggt ggtggaatac ttacttcttg ggaagaaaaa tgtaagtaat ataccctggc
     7081 aagagattgg agataagttt aaaacagcag agcttttagg gaaattggca aatgtatttt
     7141 cagatcttcc aagtaaatca atagatgata caggaatatt caaggtggta acaggagagg
     7201 attatttaat ggcagaaaag aaaaataaga atccatttaa atttaagccc tttgcaaggc
     7261 ttgtctttag ttgtaatgaa ttacctagaa actatgtgga cagaacagaa ggcttttata
     7321 gaagacttat aattgtgcct tttaataggc aaatagagaa aagtaagata gataagtcct
     7381 taaagtacaa gtttcaaagg gaaaaggaag gcatattaaa ttgggcctta gaaggattaa
     7441 agaggcttta tgaaaataac tttgagttta gtgaaaatga gctgactgat ggtattaaga
     7501 aagaatataa gcgtgagaat aataatgtta tttcctttgt agaggagtgc tgtgaacttg
     7561 atggtttatt ttcatgtagc aggatagagc tttatgaatc ttataaggag ttttgcgtgg
     7621 aagcaggttt aaaagcactt tctcaaataa agtttaataa ggagctagaa ggtaatttta
     7681 atataactag atctagaagt ggaaaattaa gatcatggaa tggagtaaga attaagttag
     7741 aggatttaat aattaggtag tgtgacaggt tagtgactga tttttgacgg actttgagcc
     7801 atagttttct tagtgatacc aagggttttg acggaagtga cggacttttt ttacttctat
     7861 ataataggga aaataaaaaa tagtataaaa aaagatacat ttattaaata ccctatatat
     7921 agtgagtgaa aaatagctaa agtctggcaa agtctggcta aagctagtca tatcaatgct
     7981 ttagagtgtg acggactttt tgaaagtccg tcataaactt gtgaaagtaa gtcaaaaaaa
     8041 cattgaaagt tcaatttaga ggtgaataag agagtttaat actattattt aataggtata
     8101 attaacaatt ttgttataca ttaaggagaa aatagatata aaatataagt aaaaggtaga
     8161 aaagaaaagg aagaatatga tatgaaaaag ggaatttggg taactatttc aattttaagt
     8221 ttaggagttt ttttagcttt atcatttttt attattgcta agaaggaaat aaaggattat
     8281 aaaaacatag ggattgatct tacttatgat gaaagggtta aagaacctat tgaagaactc
     8341 ttagttaagt ttgttgattt tgattattct aaagaagagg ttaattgtga ggagataatt
     8401 tctaataagg aagttgcagc gacatataat ttaactttta attctacact taagtataat
     8461 ttagaaagtg aattgaagat gtcaaaggtt tctataaaaa gtattgtaaa agagcctgat
     8521 aatgaatata gggtaaaatt tcatagagaa tttgagataa agtttgacaa aaatagtgat
     8581 accatatcaa gtggaatgga tgattataca gcctatatag ttgaaaagaa tggagagttt
     8641 tatatagata aaattcttaa tgatgttgat tttaaccagt ttaaaaatag taagagtaaa
     8701 ataactaaat tatttaaatc agaggaagag ctttttaatg aagcaatgaa atctgaaata
     8761 gaagctagaa ggaattatga ggaatacctt aaaaatttat aattaaaaaa tctaaata
//
LOCUS       DAEVGI010000099         8807 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEVGI010000099 DAEVGI010000000
VERSION     DAEVGI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8807
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.099"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(217..756)
                     /locus_tag="K8M77_001820"
     CDS             complement(217..756)
                     /locus_tag="K8M77_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI7086309.1"
                     /translation="MKVIVRKELEEDHKTVELLVEEAFKDAEYSDHMEHVLVSKLRKG
                     ENFIDGLSLVAEREGEIVGHILLTKANIENETEKYETLALAPVSVLPKYQGIGIGGEL
                     IREGMRMAKELGYDSIIVLGHDKYYPKFGFKKAGDFGIKAPFEVPEENFMAIELNEEA
                     LKNISGVVRYANEFLADNK"
     gene            1228..1893
                     /locus_tag="K8M77_001821"
     CDS             1228..1893
                     /locus_tag="K8M77_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBI7086310.1"
                     /translation="MESKKKILRSVLLIALGSSILAFGSYNFNYQNNVTEGGVLGLLL
                     LVKNVFDISPSITNLIIDFSLFAIGSKFFGKKFLACSIFATFCFSVSYRFWESIGFLT
                     PNFTNNMLVASVLAGIGVGVGAGIVLRGGGASGGDDVIALLGNKFTSLKVQHVYMLTD
                     AIVLLMSLVYLDFKQIFFSIIAVCISGKLISVIYEYKNDGINTKDENKEAEVLEKNGS
                     LTV"
     gene            complement(2076..2519)
                     /locus_tag="K8M77_001822"
     CDS             complement(2076..2519)
                     /locus_tag="K8M77_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7086311.1"
                     /translation="MSNIYEFKKEYLNECAELFVKTFSKEPWNEPWDFENAKKRLNDV
                     VLTPGFRGAVLRNDEKIEGVILGNLEQWYDGEHFCVKEFFVDSSSQGKGTGKKLLNAL
                     ENILMEKEVGVIHFWTMKGSTAEAFYNKRGYEIPKELIMMRKKLK"
     gene            complement(2623..3750)
                     /locus_tag="K8M77_001823"
     CDS             complement(2623..3750)
                     /locus_tag="K8M77_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI7086312.1"
                     /translation="MADQRLNKLAKLLVNYSTAVKEGEFVFVSCNEVANPWLTEVVKE
                     ATKAGAHVEYILESEEAKEARLKFSTKDQLLSGNLIMETMLEKADVWLSAWGARNTRA
                     FSNIDSEKIKNSRAGEKGWRKFYSGRMGDGSLRWCGTQFPTYADAQEASMSFSEYEDF
                     VYGAGLLDHEDPVAEWNRVSKEQERWVKYLDTKKELHILTEGTDIKVSVEGRKWINCD
                     GRVNFPDGEIFTSPVENKINGHITFSFPGIYAGKEIEGIELEVKDGKVVSYKAKKGED
                     LLKALLETDEGASHFGEVAIGTNYGIKKFTRNMLFDEKIGGTVHMAIGDSMPEAGGKN
                     RSSLHWDMLCDMRNGGKIYADGELFYENGEFKKEILEKYNI"
     gene            complement(4123..5898)
                     /locus_tag="K8M77_001824"
     CDS             complement(4123..5898)
                     /locus_tag="K8M77_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBI7086313.1"
                     /translation="MKVTERLEKLRKIMKDKGIDYYIIPSEDAHQSEYVCEHYRGRAY
                     MSGFTGSAGTLLVGLENAILWTDGRYFIQALDELKGSGIEMFKMRIPGWPSLLEWLKE
                     NAKAGETIAFDGKVFSVGEYKDFKKLEEGNKLNIKIDEDLLDEVWKERPTLPKEKAFL
                     HEVKYCGKSAREKLREVREEMKKLGANNYIIASLDDIAWLYNIRGNDIKCNPVVLSYA
                     LVKENEAYLYVDKSKFTSKMEEELLNEGVTLKSYDEIGNAISNLEGKILIDPNKISAY
                     LYECIKDKNNIVEFGNITTKFKAIKNEVELDNLRKCQVRDGVAMVKFMKWLKDNIGKI
                     EISEISASDKLEELRSLDRLFKGISFETIAGHKEHGAMMHYSATKESDYTLEPRGFLL
                     IDSGGQYLDGTTDITRTFVLGELTEEERKDYTLVLKGHIGLMRAKFLKGATGSALDIK
                     AREPLWNEGIDYKCGTGHGVGFFLNVHEGPQSISPVPNKVALEPGMIITNEPGVYREG
                     KHGIRTENTMVVVKDTYSEEFGEFYKFDTISLCPIDLAGLDISLINEEEKAWLNNYHK
                     KVYYLLSPYLDEEEKEFLKNETREI"
     gene            complement(6145..6714)
                     /locus_tag="K8M77_001825"
     CDS             complement(6145..6714)
                     /locus_tag="K8M77_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7086314.1"
                     /translation="MLDKSIEFFKIILKREKGRELKNETLPEGFRFVKFKKGDEKAWA
                     EIEKSVLEFENVKDGEEYFKNKYLPYIDELERRTIFIENNNGEKIATFTAWWRYTGER
                     RHPFMEWVAVKPEYQGKGLGKALISEGVKLMIAIEGGCDIYIPTQTWSYKAIRLYRWA
                     GFEFETEERFPGGIKNETLEGLKVIKNLI"
     gene            7259..8419
                     /gene="alr"
                     /locus_tag="K8M77_001826"
     CDS             7259..8419
                     /gene="alr"
                     /locus_tag="K8M77_001826"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBI7086315.1"
                     /translation="MDICVRPVWAEIDLDIIANNMKEIRNLVGEKEIIAVVKANAYGH
                     GALDIASTLLENGASRLAVAIITEADELRDAGITAPIMILGYTPINFAENLINNEIEQ
                     TVYDVKYAKELSDFALKLGKKAKIHIAIDTGMGRIGFLPNEEGLNKVLEICSLPGVEV
                     VGLFTHFSTSDEKDKTYTYEQFSKLTAFNKALEDNGIHIPLKHASNSGAIMDLPETYL
                     DGVRCGIISYGYYPSEEVKKENLKLKPALTLKTNVAFVKELDEDMYVSYGRTYKTEKK
                     SKIATLPIGYADGYSRLLSGKAKVIIKGQFANVIGRVCMDQCMVDVTHIEDVKIGDEV
                     ILLGEENGLKFDANDMAEIMGTINYEILCMISHRVPRIYKKNNEIVKVRNYI"
     gene            complement(8529..8708)
                     /locus_tag="K8M77_001827"
     CDS             complement(8529..8708)
                     /locus_tag="K8M77_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086316.1"
                     /translation="MGYIIILLSPILGLIFSEIYIFLTSFMPWMSADGFVTSTSVIFA
                     ILGLIIVLNDKEKTK"
BASE COUNT         2927 a         1509 c          921 g         3450 t
ORIGIN      
        1 taaatattta aattctaatt gtatattgat gtgttctcct atatctatat aaaaaaacaa
       61 aagtttaaaa ctttaatcta aaaaataaca aggtgattga taaaaaaatt ataagactta
      121 taattttttt atacaaccac cttattattt taccttaaat taataaagct atagtctcaa
      181 tatttaatta atatttaaat aaatatatca aagttattac ttattatctg ctaaaaattc
      241 attggcatat cttacaacac cacttatatt ttttaaagct tcttcattta attctattgc
      301 cataaagttt tcttctggga cttcaaaagg agcttttatt ccaaaatctc cggctttttt
      361 aaatccaaat ttaggatagt atttatcatg tcctaaaact attattgaat catatccaag
      421 ttccttagcc attctcattc cttctcttat aagctctcct ccaattccaa taccttgata
      481 tttaggtaat actgaaactg gtgctaaggc taaagtttca tatttttctg tttcattttc
      541 tatattagct ttagttaata atatgtgtcc tactatttct ccctctcttt cagcaactaa
      601 agataaacca tcaataaagt tttctccctt tctaagttta ctaactaaaa catgttccat
      661 atgatcactg tactcagcat ctttaaaggc ttcttctact aaaagctcta ctgttttatg
      721 gtcttcttct aactcttttc taacaattac tttcataact ataacatcct ttctcatatg
      781 ttttattctt aaatttaaaa attaagatat attttatgta aaataatcac ttttaaattt
      841 aagaaccata tatgtatttg ctttcattta tagtatttca aataaaaata aatttatata
      901 tattaaccat ttattcattt gaagttttct ctctattaac aacaatattt taacctttcg
      961 gaaaaccttg taaaaatgaa cactttaaag aaaaacagat gaccacagag gctatttatt
     1021 tctattaaag aattgcccct ttaatcctta ttactcttat tttatatagt ttgctcttaa
     1081 tatttattaa gattataatt aaaatataca gtaatttact ataaaatata atttaaaatt
     1141 cataattttt tatttctata tagtagacat atgaggtctg ctattttctt tatagctaaa
     1201 ttaccttaat tttaggagag tgatattttg gaatcgaaaa agaagatctt gagaagtgtt
     1261 ttattaatag cactaggttc aagcatctta gcctttggta gctataactt taactatcaa
     1321 aataatgtaa cagaaggtgg agtcttaggc ttattacttt tagtaaaaaa tgtttttgat
     1381 atatctccat ctataacaaa cttaataatt gatttttcat tatttgcaat aggttctaaa
     1441 ttctttggca aaaaattctt agcatgttca atatttgcaa ctttttgctt ctcagtatcc
     1501 tatagattct gggaaagcat aggattttta acacctaact ttactaataa tatgctagtt
     1561 gctagtgttt tagcaggtat tggagttggt gttggtgctg gaatagtatt acgtggtggt
     1621 ggtgcttctg gtggagatga tgtaatagcc ttattaggta ataaatttac ttctcttaag
     1681 gtacaacatg tctacatgtt aacagatgcc attgttttat taatgtcttt agtttattta
     1741 gactttaagc aaatattctt ctcaataata gcagtatgta taagtggtaa actaataagt
     1801 gtaatctatg aatacaaaaa tgatggtatt aatactaaag atgaaaataa agaagctgaa
     1861 gttttagaga aaaatggttc tcttacagtt taataatata tagaatttga attaaatatt
     1921 aaaaagagca tgtattaatg ttttaaactt attacatgct cttttatatc ttctatatta
     1981 aaatttaaca tatagtatta ttttaaattt tttaatttct atgtgtatta aaataatttt
     2041 aattgtcaac tagaccttca ttatttaaaa atttactatt ttaacttttt tctcatcatt
     2101 attaattctt ttggaatttc ataacctctc ttattataaa aggcttcagc tgtgcttcct
     2161 ttcatagtcc aaaagtgaat tactcccact tccttttcca taagtatatt ctctaaagcg
     2221 tttaaaagtt tctttcctgt tcctttcccc tgtgaacttg aatccacaaa gaattcctta
     2281 acacaaaaat gctctccatc ataccattgc tctaaatttc ctaagataac cccttctatt
     2341 ttttcatcat ttcttaaaac agcaccccta aatcctggag ttaaaaccac atcattaagt
     2401 ctttttttag cattttcaaa atcccaaggt tcattccacg gttccttact aaaggtttta
     2461 acaaaaagct cagcacattc atttaagtat tcttttttaa actcatatat attactcata
     2521 attcttatac tcctattatt ttaaaattaa actaaaatta aattaggaga tgagtctctt
     2581 catcccctaa tttaattatt attctataat ttatatataa tattaaatat tatatttttc
     2641 taatatttct tttttaaact ctccattttc atagaaaagt tctccatctg catatatttt
     2701 tccaccattt ctcatgtcac aaagcatgtc ccaatgaagt gatgatctat ttttaccacc
     2761 agcctctggc atagaatctc ctatagccat atgaactgtt cctcctattt tctcatcaaa
     2821 tagcatattt ctagtaaact tcttaattcc atagtttgta cctatagcta cttctccaaa
     2881 atggcttgct ccttcatcag tttctaataa agcctttaat aaatcttctc cttttttagc
     2941 tttatatgaa acaactttac catctttaac ttcaagctct ataccctcta tttcctttcc
     3001 tgcataaata cctgggaatg aaaaagttat gtgtccattt atcttatttt caactggtga
     3061 tgtaaatatt tcaccatctg ggaagtttac tctaccatca caatttatcc actttcttcc
     3121 ctctactgag accttaatgt cagttccttc tgttaatata tgaagttcct ttttagtatc
     3181 taaatattta acccatcttt cctgctcttt gcttactcta ttccattctg ccacaggatc
     3241 ttcatggtct aaaagacctg ctccataaac aaagtcttca tattcactaa aactcatgga
     3301 agcttcttga gcatctgcat atgtaggaaa ttgagttcca caccatctta aagagccatc
     3361 tcccattctt cctgaataga attttctcca tcccttttct ccagctctgc tattttttat
     3421 tttttctgaa tctatattgc taaaggctct agtatttcta gctccccatg cacttaacca
     3481 aacatctgcc ttttcaagca tagtttccat tattaaattc cctgataata attgatcctt
     3541 tgtagaaaat ttaagtcttg cctccttagc ttcttctgat tctaaaatat actcaacatg
     3601 agctcctgcc ttagtagctt cctttactac ctcagtaagc caaggatttg caacctcatt
     3661 acaagataca aaaacaaact ccccttcttt aactgctgtt gaataattta caagcagttt
     3721 agctaactta tttaatcttt gatctgccat atatatccct cctaaacata caaatttata
     3781 tattttaatt tttgaactct attttaactt actatttttt tattaataac aataaatttt
     3841 taaagctaat tttctaatct tatcttaatc tttttctttc tttaatattt aaatttaaaa
     3901 aataaaaatt ataaataata cctttattct ctttttagat aaaagctttc ctttagacaa
     3961 accttttctc tctccataaa atcacccatt tgtttatatc tatttcataa gttttatcta
     4021 ttaattttat tatactccta taaaaatttg gaaactatat aaatataaaa gaaggtggaa
     4081 gaattattcc atcttctttt ataaatctat tcatatgtta aattatattt cccttgtttc
     4141 attctttaag aattcttttt cctcttcatc taaatatggt gataataaat aataaacctt
     4201 tttatgataa ttatttagcc aagccttttc ctcttcattt attaaactta tatctaatcc
     4261 tgctaagtct attggacaaa gtgaaatagt atcaaactta taaaattctc caaactcttc
     4321 tgaataagta tctttaacaa ctaccattgt attctctgtt cttattccat gttttccttc
     4381 tctataaact ccaggttcat tagttatgat cattcctggc tccaaggcaa ccttatttgg
     4441 tactgggctt atgctttgtg gtccttcatg aacatttaag aaaaatccaa ctccatgacc
     4501 tgttccacat ttataatcaa ttccttcatt ccataatggc tctctagctt ttatatcaag
     4561 ggctgatcca gtagctccct ttaagaattt agctctcata agtcctatat gtccttttaa
     4621 aaccaaagta taatcttttc tctcttcctc agttaattct cctaaaacaa aagttcttgt
     4681 tatatctgta gttccatcta agtattgtcc acctgaatct attaataaaa atcctcttgg
     4741 ttctaaagtg taatcactct ctttagttgc tgaataatgc atcatagcac catgttcttt
     4801 atgacctgct atagtttcaa aactaattcc tttaaataac ctatctaaac ttctaagttc
     4861 ttctaactta tctgaagctg atatttcact tatttctatc tttccaatgt tatccttaag
     4921 ccatttcata aacttaacca tggctactcc atctcttact tgacactttc ttaagttatc
     4981 taattcaacc tcatttttaa tagccttaaa cttagttgtt atgtttccaa attccacaat
     5041 attattttta tccttaatgc actcgtataa ataagcactt atcttatttg gatcaattaa
     5101 aatcttccct tctaaattac taatagcatt tcctatttca tcatatgatt ttaaagtaac
     5161 tccttcattt aaaagttctt cctccatttt agaagtgaac tttgatttat ctacataaag
     5221 atatgcttca ttttctttaa ctaaggcata gcttaaaact acaggattgc atttaatatc
     5281 atttcctcta atattataaa gccaagctat gtcatctaaa gaagctataa tataattatt
     5341 agcgcctagc tttttcattt cctctctaac ttctcttaat ttttctctcg cactttttcc
     5401 acagtactta acttcatgta aaaatgcctt ttccttagga agagttggtc tctctttcca
     5461 aacctcatct aaaaggtcct catctatttt tatattaagc ttatttcctt cttctaattt
     5521 tttaaaatct ttatactctc ctacggaaaa tacctttcca tcaaaagcaa tagtttctcc
     5581 tgcctttgca ttttctttaa gccattctaa taagcttggc catcctggaa ttctcatttt
     5641 aaacatttca attccagaac cctttaattc atctaaagct tgtatgaaat atctaccgtc
     5701 agtccacaaa atagcatttt ctaaaccgac aagtaaagtc cctgctgatc ctgtaaaacc
     5761 tgacatatat gctctccccc tataatgttc gcacacatac tcactttgat gagcatcttc
     5821 actaggaatt atataatagt caattccttt atccttcata atttttctta atttttctaa
     5881 tctttcagtc accttcataa ttatagcccc ctattttttt aatctaaatt gtaatttgat
     5941 ttaacttttc aaacctcaat ttaattttct gctttcaata atagtaacat aaaaatattc
     6001 tcctaaaaat accagttgaa atttttcata atacttatta tagctttaac tattttgtgt
     6061 taaaaatcca aaataataac cctaaaattc taataaaata ttaaatgttt cttttttgac
     6121 tttattttag aatatcctta aaattcatat taagtttttt attactttaa gaccttctaa
     6181 agtttcattt ttaattccac ctggaaacct ttcttcagtt tcaaattcaa accctgccca
     6241 tctatataac ctaattgctt tataactcca agtctgagtt ggtatgtata tatcacaacc
     6301 tccttcaata gctatcataa gtttaacccc ttctgaaata agagcttttc ctaatccttt
     6361 tccttgatat tcaggtttta cagcaaccca ctccataaat ggatgtcttc tttcccctgt
     6421 gtatctccac caagctgtaa atgtagcaat tttttctcca ttattatttt ctataaatat
     6481 agttcttctt tctaactcat ctatataagg taaatatttg tttttaaaat attcttctcc
     6541 atctttaaca ttttcaaatt ccagtacaga tttttctatt tctgcccaag ctttttcatc
     6601 acctttttta aacttaacaa atctaaaacc ctctggtaaa gtttcatttt ttaattctct
     6661 tcccttttct ctctttaaaa ttattttaaa aaattctata cttttatcta acatttatat
     6721 cccctccaga taaacaatat aacctattga aacttaattt ctatccttat agatacttat
     6781 taataagact ataagtaatt tcttatacct ttctatattt aattttatca ttaaacttat
     6841 aattttaata ttacactact ttttttcatt ctttatatct agatattaat tgatccaaag
     6901 aaaataataa tttaattaag taaatacctt ttcaacttag tatttgtata tctacagaaa
     6961 tccctattaa tttttagata ttaatagaga tattttaact cactcataaa aaagtatgac
     7021 atattcttaa tattgaaacc actatatata aaaattacaa ttatggtggt tcttcacttt
     7081 atgctacaat atcttataat agtatatgta gttaagtttt ttaataaaaa tatggtgatt
     7141 tatatgtaat actttatatg agcataataa tttagctaca acttattaat ttaatgattt
     7201 aagtgaaatt aattagatta aagtaataaa agctataatt aatttgaggt gttttaaaat
     7261 ggatatatgt gttagacctg tatgggctga aatagattta gatataatag caaataatat
     7321 gaaagaaata agaaacctag ttggtgaaaa ggaaataata gccgttgtta aggctaatgc
     7381 ttatggtcat ggagctttag acatagcttc cactctttta gaaaatggtg cttctagatt
     7441 agcagtagct ataatcacag aggctgatga acttagggat gctggaataa ctgcacctat
     7501 tatgatttta ggctatactc ctataaattt cgctgaaaat ttaatcaata atgaaattga
     7561 acaaacagtt tatgatgtga aatatgctaa ggaattatct gactttgcct taaagcttgg
     7621 caaaaaagct aaaatacaca ttgcaataga tacaggaatg ggtagaatag gtttccttcc
     7681 aaatgaagaa ggattaaata aagttttaga aatatgctct ttacctggag ttgaggtagt
     7741 aggcttattt actcacttct caacatcaga tgaaaaagat aaaacttata cttatgaaca
     7801 attttcaaaa ttaactgctt ttaataaagc tttagaagac aatggaatac acattccttt
     7861 aaaacatgct tctaatagtg gggctattat ggatttacca gaaacttatt tagacggagt
     7921 tagatgtgga attatttctt atggatacta tccatctgaa gaagtaaaaa aagaaaactt
     7981 aaaacttaaa ccagcactta cattaaaaac taatgtagcc tttgttaaag agttagatga
     8041 agatatgtat gtaagctacg gaagaactta taaaactgaa aagaaaagta aaattgccac
     8101 tcttccaata ggatatgctg atggatactc aagacttctt agtggaaaag ccaaagttat
     8161 aataaagggt caatttgcta atgttatagg aagagtttgt atggatcaat gtatggttga
     8221 tgtaactcat atagaagatg ttaaaattgg tgatgaagta attcttttag gggaagaaaa
     8281 tggattaaaa tttgatgcta atgatatggc tgaaattatg ggaacaataa actacgaaat
     8341 actttgtatg attagccata gagttcctag aatatataaa aagaataatg aaatagttaa
     8401 ggtaagaaac tatatttaat aaaaatagga gtgtattaaa agttatttta atacactctc
     8461 ttatttagtc attaaaattt aaataaactc taacctaatt taatcataat tatttatagc
     8521 actaattttt attttgtttt ttccttatca tttaacacta ttataagtcc taaaatagca
     8581 aaaatcacac tagtggatgt aacaaaacca tctgcagaca tccatggcat aaatgaagtt
     8641 aagaaaatat atatttctga aaaaattaat cctaaaattg gacttaataa aattataata
     8701 taccccatta atcctcttct ataattaaac ctatttttct aaaaatctat tattaaacaa
     8761 gttttttctt tttattatat ctttaaaata attaatttct gtttaaa
//
LOCUS       DAEVGI010000100         8748 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEVGI010000100 DAEVGI010000000
VERSION     DAEVGI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.100"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            84..1236
                     /locus_tag="K8M77_001828"
                     /pseudo
     CDS             84..1236
                     /locus_tag="K8M77_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1398..1583
                     /locus_tag="K8M77_001829"
     CDS             1398..1583
                     /locus_tag="K8M77_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBI7086317.1"
                     /translation="MGNIDKDRIVCRCKKVSEGTIIEAIKNGADTYEKVKKETGANTY
                     GCFACRLEIKKLLEENK"
     gene            complement(1684..3021)
                     /locus_tag="K8M77_001830"
     CDS             complement(1684..3021)
                     /locus_tag="K8M77_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7086318.1"
                     /translation="MFGFFSPAPAIPRLPKDKVSSAYKAYRIRMFLMIFIGYTGYYLV
                     RKNFAVASPYLIDNYGFSKTQIGLISMGLAVSYGLSKFILGNLSDKSNVKKFICFGLI
                     ASSVLSVLMGFARTVPIFLILMVFNGFFQGMGAPPCSIVLGKWFSQKERGLKMGIWNT
                     SHNIGGGLIAPIATLCLLIFGQNHFQSIFFVPAIICTIVALFCYVIGADTPASVGLPP
                     VEEYRNDYPEVKAEVNPTTLSSKEIMVKYVLKNKYVWYLALANIFVYLIRQGVVNWIP
                     IYLKESKGFSAANANWAMFLFEYAAIPASILLGWLSDVVFKGKRAPLSILCMIGVIFA
                     TVAYWQTSSTLVTMISVAFIGCFIYGPQLLIGMNLIDVVPNFAVGSATGFSGLCGYLL
                     GEFMADFVLGMIADSFGWNGAFIFIIVGAFIALLLLALTLKLKKNPQVEDKIA"
     gene            complement(3222..4241)
                     /locus_tag="K8M77_001831"
     CDS             complement(3222..4241)
                     /locus_tag="K8M77_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI7086319.1"
                     /translation="MKKFLNKKITIILTIIILLLIAKLHIIKKEKALSTSNMNHIVVY
                     GSLREEEGKYLLEKFKNEYDCTYEYIKLPTEEAVQRILDTRDNPQGDIFIGGSCDGYE
                     ILKKYDALEEYNSPWANNIPAKYKDLRGYWTGFQITPLSIGINKSLWQTYFGDSVPYP
                     KKIEDLLNPEFKDKIILPNPQTSGTGYTLMASLYQELGKEKFEAFIAKLNDNVSSSTI
                     SGFNSIQRVASGEYLITVNFLSDQLIANRSSDNIISIVPENAGWNIDSIALIKNSKHE
                     KIAKSFIDFVLSKETSSDLSKFSKAISTKNFDTNQFKIYDKYNFYLAAEDREKIMQIL
                     YENKE"
     gene            complement(4222..5010)
                     /locus_tag="K8M77_001832"
     CDS             complement(4222..5010)
                     /locus_tag="K8M77_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3919 family protein"
                     /protein_id="HBI7086320.1"
                     /translation="MSKNFKNILAIYLFFIVIILSTYIYYSSNIYDSLTVVKSSQENK
                     GDLNIKIPDKIILSNPYLGTTVISNKSELNELLQYFNKICLTNSTKENALPLENTLFI
                     SGEIHYLNGDKTPFEISNILKLDGESYSNNSSLINMLRNNLKDAFYTVNNLESILSSP
                     DNLITYKEDGTMKNLSKDSKSLLLEDFKSLRLMNDNKDFLKTDLSEIPKHHLTLYRYA
                     KEGDISNNTFYIDVYNDYVIIQYLGDENGKNIYLKGNLNEEIFK"
     gene            complement(5003..6433)
                     /locus_tag="K8M77_001833"
     CDS             complement(5003..6433)
                     /locus_tag="K8M77_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7086321.1"
                     /translation="MKNNKKQMNLNLKQKIILTNLLILAPIIIFIFIITYNALSKNLI
                     NNSIEYLLDKSRTTESYILNLLSSNDSEDKEDIIRDNAPFIASTLSEKYNVRIQIISN
                     SAQIIYDSSSDEISLFGSDINEALDGQTAYIVEKIDNVPTLFLSSPIFYKGKEYGAIR
                     LILINTEAYEILTNTLTIFIISGLIAIIIAIFLINTFAKELVSPLMILKQKSKNISEG
                     KFKEELHIKSGDEVEDLANTFNIMSENLDKYINEIKSAKLHQKKFFDNVSHEFKTPLT
                     AIIGFSEIIPKLNDKEKIVQSSALIEKEGKRLLNLVEEILLLAKSNKNTFEIEYTYID
                     VKSLIDDCLKILKIKLDNYDIKVYRNYESFFIYGDHDKTKQVFINIIDNAIKYSGCET
                     LNISTKRSPNKMEVIIEDDGTGFIIKDEYVNPKGNGLGIKICKDIMQSQNYGFNIESE
                     LDVGTKVTLSFSIGKNNETKEGDFNE"
     gene            complement(6423..7094)
                     /locus_tag="K8M77_001834"
     CDS             complement(6423..7094)
                     /locus_tag="K8M77_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7086322.1"
                     /translation="MKILVVDDEVSILQLIKMNLEIEGHIPITAENALDALELVIKER
                     PDIIILDAMLPDISGFNLIPKIKNIDDIPIIMLTAKSDINDKLLGLQLGADDYITKPF
                     NSTELILRINIVGKHISSDSKKKVKDLTIGDLTLLLDERKALIKSEYINLTFKEFEVL
                     RCLCQNKNKVFSREELLNKVWGYDFEGTTRAVDILIQRLRKKLGPCQSYIKTLYKAGY
                     KLDEK"
     gene            7384..8583
                     /locus_tag="K8M77_001835"
     CDS             7384..8583
                     /locus_tag="K8M77_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBI7086323.1"
                     /translation="MISYEFLFDLALILISTKLFGLITKKIRMPQVVGALVAGVVLGP
                     AFLNVLSETEFIQNLAELGVIVLMFTAGLETDINQLKKTGKASFIIAVLGVIIPLVGG
                     FFIASIFNKGNDVNTILQNVFIGIILTATSVSITVETLKEMGKLNTRAGNAILGAAII
                     DDILGIIALTITTSLADPSINVIIVLIKIVMFFIFAGFAGYLFHWAFIKLDEKYQRDL
                     RRFVIIAFVFCLLLSFCAEEFFEVADITGAFIAGLVISDSNRSKYLNSRFETLSYMLL
                     SPIFFASIGIKIKLTAMTKTIFIFSILLLLVAILSKVFGCALGAKICRYSNIEAIQIG
                     TGMISRGEVALIVANKGIAMGLMLQEFLASVVIMVVVTTIVTPILLKVVFKNRSKSVD
                     LNLKANV"
BASE COUNT         3135 a         1132 c         1269 g         3212 t
ORIGIN      
        1 tagaattttt tagataatat tttaatgata aaggaataat attagatgaa attattaaag
       61 ttttaagaaa aaggttagag aggatgaaga agttgaaaag ggcttacaag gtggagatta
      121 atcctactga tgaacaaaag tctaaaatac accaaactat tggtgtatca agatttatct
      181 ataactttta tattgctcgt aataaagaaa tttatgagag agaaggaaag tttgtaagtg
      241 gtatggattt ttcaaaatgg ttaaataatg agtatattcc ctaaaaatca agatatgaaa
      301 tggattaaag aagtatcttc aaaagctact aaacaagcta ttatgaatgg agataaagct
      361 tttagaaatt tctttaaaaa atctaaaggc tttcctaagt tcaagaaaaa gaaaaatcaa
      421 gatgtaaaag catattttcc taagaataat aaaactgatt ggactttaga aagacataga
      481 gtcaaaatac ctactcttgg atggataaag tttaaagagt ttggatacat acccgctaat
      541 tcagtagtta aaagtggaac agtaagtcaa aaatccaata gatactacgt ttcaatatta
      601 gttgatgaag atgacattca ggtatctaac ccaataaatg aagggatagg tattgatgtc
      661 ggtataaaag atttcgcaat atgttcagat ggagttaaat ttaaaaatat aaataaaaca
      721 tctactgtca aaaaagtaga aaagaaatta aaaagagaac aaagaaaact ttcaaggaaa
      781 tatgaaagtt taaaagaagg gagggctact cgtcaaaata ttcaaaaaca agtaatcaaa
      841 gtacaaaaac ttcatcagag attaactaat atacgaactg attatataaa taaaactata
      901 tctttaatta taaagcaaaa accaagctat ataacaattg aagatttaaa tgttaaagga
      961 atgatgaaga ataagcattt atctaaagct attgcaagtc agaagttttt tgaatttaaa
     1021 actaaactaa tgtctaaatg taaagaaaat aatatagagc ttagagtagt ggatagattt
     1081 tatccatcat caaagacttg tagtcaatgt ggagagatta agaaaggttt aaaactatta
     1141 gatagaacat acaaatgtga ttgtggactt actattgata gagatttaaa tgcaagtatt
     1201 aatcttaaaa atgctaaaaa atataagata gcttaaaatc aaaagcactt atatatgtac
     1261 ggagggctaa cttcggaatt tacgcctttt gagaaccatg caaaatcgta gtagcttagg
     1321 caaggcgagg ttctatgaag aaggaataat ctccatatgg atatatttga ccatattttg
     1381 agtagcagat ttttagaatg ggtaatatag ataaagatag aattgtgtgt agatgcaaaa
     1441 aggtatcaga gggaaccata atagaagcaa taaaaaatgg agcagatact tatgaaaagg
     1501 taaaaaaaga gacaggagcc aatacttatg ggtgttttgc ttgtagatta gaaataaaaa
     1561 aattattaga agaaaataaa tagaagaaaa tatttatata aaaggaaact agaaaggggg
     1621 agcctcctag tttcctttat ctgatttatt atgtatatat ttagttatat tgtgattaaa
     1681 atattaagct atcttatctt caacttgtgg atttttcttt agctttaaag ttaaagctaa
     1741 aagaagtaat gcaatgaatg cacctactat gataaatatg aatgcaccgt tccatccaaa
     1801 gctatcagct atcataccta aaacgaagtc ggccatgaat tcaccaagaa ggtatccgca
     1861 aagtcctgag aaacctgtag cagaaccaac agcgaagtta ggtacaacgt ctattaagtt
     1921 cattcctatt aataattgag gtccgtatat gaagcatcct ataaatgcaa cagaaatcat
     1981 tgttactaat gtactgcttg tttgccagta agctactgta gcaaatataa caccgatcat
     2041 acataagata ctaagtggag cacgctttcc tttaaataca acgtctgaaa gccatccaag
     2101 taatattgat gctggtattg cagcatattc aaataagaac attgcccagt ttgcattagc
     2161 agcagagaat ccttttgatt cttttaagta tataggaatc cagttaacaa ccccttgtct
     2221 tattaaataa acaaagatat tagctagtgc taagtaccaa acatatttat ttttaagaac
     2281 atatttaacc attatttctt ttgaagataa agttgttgga ttaacctcag ccttaacttc
     2341 aggataatca tttctatatt cttcaactgg aggtaatcca acagatgctg gagtatcagc
     2401 cccaattaca taacagaata aagctactat tgtacatatt attgctggta caaagaatat
     2461 tgattggaaa tgattttgtc caaatattaa taaacataaa gttgctatag gtgctattaa
     2521 acctccacct atattatgag aagtattcca tattcccatt ttaagtcctc tttctttttg
     2581 agagaaccat ttacctaata ctattgaaca tggtggagct cccatacctt ggaagaatcc
     2641 gttaaatacc ataagtatta agaatattgg aactgtacga gcaaatccca ttagaacact
     2701 taatactgat gatgcaataa gtccaaaaca tataaatttc tttacgtttg atttgtctga
     2761 taaatttcct aaaatgaact tacttaaacc ataagataca gcaagtccca tacttataag
     2821 tccgatttgt gtttttgaaa atccatagtt atcaattaaa tatggtgatg ctacagcaaa
     2881 gttttttcta actaagtagt aacctgtata tccaataaat atcataagga acatacgaat
     2941 acgatatgct ttgtatgctg atgatacctt atcttttgga agtctaggta ttgctggagc
     3001 tgggctaaaa aatccaaaca ttctataatc ctcctagtta tatgtgttat acgtgttaat
     3061 gttttcaaaa aacagtttac ttattaataa taaaataaga attaaacaac aaaatctctt
     3121 aaatgtttca taaatgtaac atttaagaga ttttaattaa ttttaatcct atgattttta
     3181 ttaaataaat ttcacttaca tataagttat tattatttaa attactcttt attttcatag
     3241 agtatttgca ttattttttc tctatcttca gcagccagat agaaattata tttatcatat
     3301 attttaaatt gatttgtgtc aaagttctta gtagaaatag ctttagagaa ctttgataag
     3361 tcacttgaag tttcctttga taatacaaag tctataaagc ttttagctat tttttcatgt
     3421 ttagaattct taattaaagc tattgaatct atattccaac cagcattttc agggactatg
     3481 ctaattatat tgtctgaaga tctattagct attaattggt cacttagaaa gttgaccgtt
     3541 ataagatatt ccccagaggc aaccctttga attgagttaa atccacttat ggttgaagaa
     3601 gatacattgt cattaagctt agctataaac gcttcaaact tttctttccc tagctcttgg
     3661 taaagtgatg ccattaaggt atatcctgta ccagaggttt gaggattagg aagtattatt
     3721 ttatccttaa attctggatt taataagtcc tcaattttct tagggtaggg aacagaatct
     3781 ccaaaatatg tttgccatag agatttgttt attcctattg atagtggagt tatttgaaaa
     3841 ccagtccaat atcctcttaa atccttgtat tttgcaggaa tattattggc ccagggagaa
     3901 ttatattctt ctaaggcatc atactttttc aaaatttcat atccatcaca gcttccccca
     3961 atgaaaatat ctccttgagg attatccctg gtgtctaata ttctttgcac agcttcttcc
     4021 gttggaagct ttatatattc ataagtacaa tcatattcat ttttaaattt ttcaagaaga
     4081 tattttccct cttcctctct aagagagcca taaacaacta tatggttcat gttagatgtg
     4141 cttaaagcct tttctttttt tataatatgt aactttgcta ttaatagtag aattataata
     4201 gttaatatta tggtgatttt tttatttaaa aatttcttca tttaaattcc cctttaaata
     4261 aatgttttta ccattttcat cacctagata ttgtatgatc acgtagtcat tataaacatc
     4321 tatataaaaa gtattattgg agatatctcc ttctttagca tatctatata aagttaaatg
     4381 atgtttaggg atttcactta aatcagtttt taaaaaatcc ttattatcat tcataagtct
     4441 taaggactta aagtcctcta ataaaagaga cttagaatcc tttgatagat ttttcatagt
     4501 tccatcctct ttataggtta ttagattatc aggacttgat aatatggatt cgaggttgtt
     4561 aacagtataa aatgcatcct ttagattatt tctaagcata tttattaaag aggagttatt
     4621 agaataactt tcaccgtcta attttaatat gtttgatatt tcaaagggag ttttatctcc
     4681 atttaaataa tgaatttcac cagatataaa taaggtgttt tctaagggca aagcattttc
     4741 tttggttgag ttagtaaggc atattttatt aaaatactga agcaattcat taagttcaga
     4801 tttatttgat attactgtgg ttcctaaata aggatttgaa agtattattt tatcaggtat
     4861 tttaatattt aaatctccct tattctcttg tgaactttta acaactgtta atgagtcata
     4921 tatattacta gaataataga tataggtaga aagtattatt actatgaaaa ataagtaaat
     4981 agcaagtatg tttttaaaat ttttactcat taaaatcccc ctccttagtc tcattatttt
     5041 taccaataga gaaggagagt gtaaccttgg taccaacatc taattcgctt tctatgttga
     5101 aaccatagtt ttgactttgc attatatctt tacatatttt tattccaagt ccatttcctt
     5161 tagggttaac atactcatct ttaattataa aacctgtgcc atcatcttct ataataacct
     5221 ccattttatt tggagatctc tttgttgata tattaagagt ttcacaacca ctgtatttta
     5281 tggcgttatc tattatattt atgaagactt gttttgtttt atcatggtct ccatatatga
     5341 aaaaggattc ataatttcta taaaccttaa tatcataatt atcaagtttt atttttaata
     5401 tttttagaca atcatctatt aaggatttaa catctatata ggtgtattca atttcaaagg
     5461 tattcttatt acttttagct aaaagaagta tttcttctac taaatttaaa agtcttttcc
     5521 cttctttctc tataagagct gaactttgaa ctattttttc cttatcattt aacttaggaa
     5581 ttatttcaga gaagccaatt atggcagtaa gtggagtttt gaattcatgg gatacattat
     5641 caaaaaactt tttttggtga agcttagctg actttatttc atttatatat ttatcaaggt
     5701 tttcactcat tatgttaaag gtgtttgcca agtcttcaac ctcatctcca ctttttatat
     5761 gtaattcctc tttgaatttt ccttcagaaa tatttttaga tttttgcttt aaaatcatta
     5821 aaggactaac tagttcttta gcaaaagtat ttattaaaaa gatggcaatt attatggcta
     5881 ttaaaccact tataataaat atggttaagg tatttgttaa aatctcatag gcttctgtat
     5941 ttattaatat aagcctaatt gcaccatatt cctttccttt ataaaatatt ggactagaaa
     6001 ggaatagagt aggtacgtta tctatttttt ctactatgta agcagtctgc ccatctaagg
     6061 cttcatttat atctgaacca aataatgaga tttcatctga ggaagaatca tatattattt
     6121 gggctgaatt actaattatt tgtattctca cattatattt ttcactaagt gttgaagcta
     6181 taaatggagc attatctcta ataatatctt ctttatcttc agaatcattt gatgaaagta
     6241 gattaagtat atatgattct gttgttctac ttttatcaag taaatattca atagagttat
     6301 ttattaaatt tttacttaaa gcgttatagg ttattatgaa gataaaaatt attataggag
     6361 ctaatattaa taaattagtt aaaataattt tttgttttag atttaagttc atttgctttt
     6421 tattattttt catctaattt ataaccagcc ttatataagg tttttatgta actttggcag
     6481 ggacctaatt tttttctaag tctttgaatt agaatatcta cagccctagt tgttccttca
     6541 aagtcatatc cccaaacttt atttagaagt tcctcccttg aaaaaacttt atttttattt
     6601 tggcaaaggc atcttaatac ttcaaattct ttaaaggtta agtttatata ttcagattta
     6661 ataagagcct ttctttcatc taatagtaag gttaaatctc caattgttaa atcctttact
     6721 tttttctttg aatcacttga aatatgtttg ccaactatat ttattcttaa aattagttcg
     6781 gtgctattaa atggttttgt tatataatca tcagcaccta attgtaaccc taaaagctta
     6841 tcatttatat cactttttgc agttagcatt attataggta tatcatcaat attttttatt
     6901 ttagggatta aattaaatcc acttatatca ggtaacatag catctaaaat tattatgtct
     6961 ggcctctctt ttattactaa ttctaatgca tctaaagcat tctcagctgt tataggaata
     7021 tgaccttcta tttctaagtt catttttatg agttgtaaaa tacttacttc atcatctaca
     7081 actaaaattt tcataactgt tcattccccc tttattagaa tttagcagca attttaattt
     7141 tagcataaaa ggttacttat taattgtact atattaattt tattgttaaa gagtaaattt
     7201 aatatttttt attagtataa gtaaagtaaa attttttcta ttaataatat aaataattta
     7261 gggtgtatta atacttatat aaaattaata tattaaaaaa tttagttata aatagggatt
     7321 gaaaaaattg aaaaataaaa ctataatgta aattaatgta tattatatga gggaggattt
     7381 gtaatgatat cttatgaatt tttatttgat ttagccctta ttcttattag cactaaatta
     7441 tttggattaa taactaaaaa aattagaatg ccacaagttg ttggggcttt agtagctgga
     7501 gttgttttag ggcctgcttt tttaaatgtt ttatctgaga cagaatttat tcaaaatctt
     7561 gcagaattag gtgttatagt acttatgttt acagcaggtt tagaaactga tattaatcaa
     7621 ttaaagaaaa caggtaaagc atcatttata atagctgtac ttggagtaat aattcctctt
     7681 gtaggaggat tttttattgc aagtatattt aataagggaa atgatgttaa tacaatattg
     7741 caaaatgtat ttataggaat tatattaaca gctacttcag tcagtattac tgtagaaact
     7801 ttaaaagaaa tgggaaaatt aaacacaagg gcaggaaatg caatactagg agctgcgatt
     7861 attgatgata ttttaggaat aatagcctta accataacaa ctagcttagc agatccatcg
     7921 attaatgtaa taatagtttt aattaaaata gttatgttct ttattttcgc tggatttgca
     7981 ggatatctgt tccactgggc atttataaaa ttagatgaaa aatatcagag agatttaaga
     8041 agatttgtaa taatagcctt tgtattttgt ttattattat ctttttgtgc tgaggaattc
     8101 tttgaagttg cagatataac aggagctttt atagcaggtc tagtaatatc tgattcaaat
     8161 agaagtaaat atttaaattc aagatttgaa accttgtcat atatgctatt atctcctata
     8221 ttttttgcaa gcataggtat aaaaattaaa ttaacagcta tgacaaaaac tatttttata
     8281 ttttcaatat tattactttt agtagctata ttaagcaagg tttttggatg cgccttagga
     8341 gctaagatat gtaggtatag taatatagaa gctattcaaa taggaacagg aatgatatca
     8401 aggggtgagg tggcacttat agttgcaaat aaaggtattg caatgggact tatgctgcaa
     8461 gaatttttag catcagtagt aataatggta gttgttacaa ctatagtaac tcctatttta
     8521 ttgaaagttg tttttaaaaa tagatctaaa agtgtagatt taaatttaaa agcaaatgtg
     8581 taaaacttaa aaataattta aaaaaattta gtgtatagaa tataaaaaaa caatgaaaga
     8641 aattaaaaaa taagctatat caaaactttt ttaataattt tttgatatag cctttttaga
     8701 catgtattat aataaaaata aaaaaaaggt tctgttttac agaacctt
//
LOCUS       DAEVGI010000101         8733 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEVGI010000101 DAEVGI010000000
VERSION     DAEVGI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.101"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            391..1014
                     /locus_tag="K8M77_001836"
     CDS             391..1014
                     /locus_tag="K8M77_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI7086324.1"
                     /translation="MKIYFTRHGETLWNLEHRFQGWKDSELTENGVKRAELLGKKFND
                     IKIDKIFTSPIKRAKRTAYLIKGDKDIEIEEVEGLKEISFGKWEGMTTKEIKSHDEYV
                     NELDNLFNKPFSYKSFEGENIKEFTERLFKTVDDIVKNNEDKDILIVTHGMCVRYLIG
                     YFKNEFEESKIIDYPIFHQASYNEVEWNGENFEVITLNNIDHYEYDK"
     gene            1900..2172
                     /locus_tag="K8M77_001837"
     CDS             1900..2172
                     /locus_tag="K8M77_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086325.1"
                     /translation="MSKKVEELKNEVMADVEKSCVEDIKAHMIDKIEEDVKVLDEDSR
                     AYKEDEKEAAKLGKAINKELDREIKEEAENAKEFAKELKADIESRE"
     gene            complement(2571..3524)
                     /locus_tag="K8M77_001838"
     CDS             complement(2571..3524)
                     /locus_tag="K8M77_001838"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBI7086326.1"
                     /translation="MIREKTNKISIIGAGFVGSTTAFALMQDGLASEIVIVDINKDKA
                     HAEAMDLAQGAAFVKSVDIKSGDYADTKDSDIVIITAGVGPKPGETRLDIINKNLKIF
                     KSIVPEVVKYSPNSILLVVSNPVDILTYITYKLSGFPKERVVGSGTVLDTSRLKYMLS
                     EHFDIDARNVHTYIIGEHGDSEITAWSLTNIAGANVDEYCKTVCANCDGSFKKELPEK
                     VKNAAYEIINSKGYTNYAVALAVTRIVEAILRDENAILTVSSLFEGQYGIDNVYLAMP
                     TIVDRSGARQILDVPISNEEKENLIKSAEILKGHIANSELD"
     gene            4137..5312
                     /locus_tag="K8M77_001839"
     CDS             4137..5312
                     /locus_tag="K8M77_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086327.1"
                     /translation="MIKIKKNINKDAEKKYEYRFYRYLGLILIILFSCIVTVSNLINS
                     DATKKLISQMVRKDYSDFFQYELKNSDRSMQGIYDLYSKRENIDVYKLMALNTIYFGG
                     DRNRYLEPYIRPYIDKYNLSQNDRLKLYEDVNKEIKNIENKYIFKLIVKRFSPYILAI
                     AFVTLMMGSASFIRKRNNAEKIIINILIFLAGIYICYLSSISVKSGIWINLAIMMALA
                     GFIIENKTKLLEYIGGSIILIILALSILGKFEYKSNELYKYDVNKLVLVNNFQGANFA
                     NEEHDIQRSGRALYSEIKKENMILVDNSNENINNLIENKEKIIFNNKENNEPVFYMCL
                     YNNIPVYRLALVKDGQIVKIYEGVLNPYMRSTLMKLERGKGHKKNKVCNIEMLKTIN"
     gene            5455..5871
                     /locus_tag="K8M77_001840"
     CDS             5455..5871
                     /locus_tag="K8M77_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086328.1"
                     /translation="MEYKKEQFNEKVLTDHITKEQWIDILKNSNLIGLRDMKILLNLY
                     GSNGQPLKTSLIGEHLEFEDISTRIQKMCKKIEEELGITLDEKSEMGSWKYKFRHWFI
                     MLNGSKIYDEKEEKNYFTWTLKKELKEAIDSLLGKE"
     gene            6079..6801
                     /locus_tag="K8M77_001841"
                     /pseudo
     CDS             6079..6801
                     /locus_tag="K8M77_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
     gene            complement(7016..8338)
                     /locus_tag="K8M77_001842"
     CDS             complement(7016..8338)
                     /locus_tag="K8M77_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBI7086329.1"
                     /translation="MSNFNETPRGSRIHISLFGKTNSGKSSIINALTGQNISLVSDFK
                     GTTTDPVYKAMELLPLGPVVFIDTAGFDDEGEIGKLRVEKTEEVVGKTDVALITLSLS
                     EILEAIKTNIEFKDMLSKEILWLNKLKKAKKPAILVINKCDLVPNKLIESKIDLKDID
                     KTTLSNKDYFVDSNLNNSLKEIGELLGIPCVAISAKNNLNINELKKELVNVSPSSITE
                     SPIIGDKIKAGDKILLVAPQDIQAPKGRLILPQVQVLRDILDYGGIPTMVTLDKLDEG
                     LKIFNGKPDLVITDSQVFKQVNKKLDRSVPLTSFSILMARYKGDLDKFYSGAKAIKNL
                     KAGDKVLIAEACTHHQLKGDIAREKLPTWLEETCPGVIVHNCSGKDFPKNLNEYALVI
                     HCGGCMFNKAEIMNRIGICDDALVPITNFGTSIAEINNILDRVMEPLK"
BASE COUNT         3346 a          888 c         1297 g         3202 t
ORIGIN      
        1 aagatgaagc tagacacgga aaagctttcg ctggattatt aaacagatac tttaaataag
       61 atataaaaat tacactcttt ataaattaaa aatttaatta cttccttatt agaagagatg
      121 ccgcaacggt atctcttttt acttatgaat aaaaagtttt ttattatgat atttttaata
      181 taaggtatat aattattaca gagtcaactg cttatgactt taaataatct ttttaagtat
      241 aattttctta atacaaataa gtaaataact atttttattt tgcaagataa tagatggtta
      301 ttgtttaaag atgattgtct taagggattt ttaagtatag ataaaattta tgtataaata
      361 agaggaatag aagggagaag tatttttaat atgaaaatat atttcacaag acatggggaa
      421 actttatgga atcttgagca tagatttcaa ggatggaagg attctgagtt aactgaaaat
      481 ggagttaaaa gagcagagtt attaggaaag aaatttaatg atattaaaat agataagatt
      541 tttacaagtc ctataaaaag agcaaagaga actgcatact taataaaagg tgataaggat
      601 attgagattg aagaagttga aggattaaaa gaaataagtt ttggaaagtg ggagggaatg
      661 accactaaag agataaaaag tcatgatgaa tacgtaaatg aattagataa tctattcaat
      721 aaaccatttt cttataagag ttttgaagga gaaaatataa aagagtttac agaaagacta
      781 tttaaaactg tagatgatat agtaaaaaat aatgaagata aggacatttt aatagttact
      841 catggaatgt gtgtaagata tttaatagga tattttaaaa atgaatttga ggaaagcaaa
      901 ataatagatt atcctatatt tcatcaagct agttataatg aggttgaatg gaatggagaa
      961 aattttgaag taattacttt gaacaatatt gaccattatg aatatgataa ataatataag
     1021 aagtttaaat aatttaatga ataaaaattt taaaattaca caacttaata gtagacttgt
     1081 gctacaatct taaatctaaa atctagtgat ttttaaatct accttcttcg attcttggga
     1141 agagggaaat agattttaga agtaagattt ttaaatattg aggtgcttta agtagcacct
     1201 tcctaatcta aaagttatag ctaaatcaaa atattagctt aaaagtttaa tgtaaaaggg
     1261 atatttagct tactgtagct tagaaagtag caataaagtt atagaaaatt atgcttttag
     1321 ataatttatg agcacattaa aaatagggat acataattgt atccttattt tataatttta
     1381 ttttcctttt gtaggttaaa tttatttata tataaaagcc ttttatatat gtttaataca
     1441 tttttttagt tttctaaaac cataggctat ttattaggct catcaatata aaatataatt
     1501 ttcaacttaa ccacttgcaa acttagatta atttctaagc tgttatataa atattaaata
     1561 agattttaaa gttctaatgt tatttctttg ttctataacc acttatagaa gtggactact
     1621 tcttatataa ttttatattc atagttttta ataatttata tttaaaactt aaatatctgt
     1681 atttttgtaa tgattataat ataaaaattt aattactttg ttttatgtaa ttttattaat
     1741 aaaatagtga taaattttaa gtagataatg tttaaattga taaaataata attattataa
     1801 agaagtattt atttatcttt ctaagagtga tataattaaa ttgtaaagaa tgttaacaaa
     1861 atgttcatta attatatttg tttttaggag ggttataaaa tgagtaaaaa agtagaagaa
     1921 ttaaaaaatg aagttatggc tgatgtagaa aaatcttgcg tagaagacat aaaagctcat
     1981 atgatcgata aaatagaaga agatgtaaaa gttcttgatg aagatagcag agcttacaaa
     2041 gaagatgaaa aagaagcagc taaattagga aaagctatca ataaagaatt agatagagaa
     2101 ataaaagaag aagctgaaaa tgccaaagaa tttgctaaag aattaaaagc tgatatagaa
     2161 tcaagagaat aatattttaa ataattaaat aaaattttca cattactttt gatgtaaaaa
     2221 gactgtatgt aatatccaga aagtgcatat agtcttttta ttttgattaa ttttaggtta
     2281 ataaatagta ataaaaaagt ataatacaat aaaaatattg cattatactc ttttttatta
     2341 ttaaaagggt tatgtaatta aggaatataa atttgttagc tttgatgcta attaatatcc
     2401 aaaatttatt tgaacattaa ttaatttcaa atactcaata aaggtagtgg aaagtttaaa
     2461 taaaaatatt tatttaaaca ttaattaatc ttaaagctta gagtatagtt tgaattttta
     2521 ttttaaactt caggtgtaag taaattaaag cttacacctg aaagtttatg ctaatctaat
     2581 tcggaattag ctatatgacc ttttaaaatt tcggctgatt ttattaggtt ttctttttct
     2641 tcatttgata tagggacatc tagaatttgt ctagctccac ttctatcaac tatagtaggc
     2701 attgccaaat aaacattatc aattccgtat tggccttcaa atagagaaga aacagttaaa
     2761 atagcatttt catcacgaag gattgcttca actattcttg taacggcaag agctacagca
     2821 tagtttgtat atcctttgct atttattatt tcataagctg catttttaac tttttcagga
     2881 agttcctttt taaaggagcc atcacagtta gcgcaaactg ttttacaata ttcatcaaca
     2941 tttgcaccag caatattagt taagctccaa gctgttattt ctgaatcacc atgttctcca
     3001 attatatatg tgtgaacatt tctagcatct atatcaaaat gttctgaaag catatatttt
     3061 aatcttgaag tatctaagac agttccagat ccaacaacac gttctttagg gaatccagaa
     3121 agcttgtaag ttatataagt taaaatatca actgggtttg atactactaa aagtattgag
     3181 tttgggctat atttaacaac ctcaggtact atacttttga agatttttaa gtttttattt
     3241 ataatatcta atcttgtttc acctggcttt ggaccaactc cagcagttat tataactatg
     3301 tcagagtctt ttgtatctgc atagtctcct gattttatat caacagattt cacaaatgcc
     3361 gcaccttgtg ctaggtccat agcttcagca tgtgccttat ccttatttat atcaacaatt
     3421 actatttcgg atgctaatcc atcttgcatt aatgcgaagg ctgttgttga accaacgaat
     3481 cctgcaccta ttattgagat tttattagtt ttttctctta tcatatatat catctcctaa
     3541 attaattcct agttaattac tctgaattat aagttaatta tataacaaat aattattatt
     3601 atcaagtagt tttctattat tttcatgttt agattattta agtagttatt tattactata
     3661 atctaagaaa ctttataaaa atattttgtc taaatagaag gatatttata cttgtaagag
     3721 aatataaata ttgaagaaaa tatatttaag agtgtaaaga tttgataatt aataagtaat
     3781 aaatttgtgg gctttaggat aaagaaaaat atctttaaaa acttaaatat attttattag
     3841 aaatattatt tatagggaaa gaagagttaa attaaaaaaa caaaatgggg tttaaagttt
     3901 ttatttaact cttataggaa ttaagaggtt gtattaaaac aatttataat ataaagctta
     3961 aattagtaat ttattaattt aaaaaaatga agttttatat taaagttttg atacaacttc
     4021 ttagtttaat aaatatataa atgaaaatat atatttataa agtgatattt tgggaaaaat
     4081 agtatataat tgaatggtga taaagagaga gaattgtttt aaaggggtga gcatctttga
     4141 taaaaataaa aaagaatatc aataaagatg ctgaaaaaaa atatgaatat agattttata
     4201 ggtacttagg tttgatttta attattcttt tctcttgtat agttactgta agtaatttaa
     4261 taaattcaga tgcaaccaag aaattaattt cacagatggt tagaaaggac tatagtgatt
     4321 tttttcaata cgaattaaaa aatagtgata gatcaatgca aggtatatat gatttatatt
     4381 caaagcgtga aaatatagat gtttataaac taatggcatt aaatactata tattttggtg
     4441 gagatagaaa taggtattta gagccttata taagacccta catagataaa tataatttat
     4501 cacaaaatga tagattgaaa ttatatgaag atgtaaataa agaaataaaa aatattgaaa
     4561 ataagtatat ctttaagtta attgttaaga gattttcacc gtacatactt gctattgctt
     4621 ttgtaacttt aatgatggga agcgcttcat ttattagaaa gagaaataat gctgaaaaaa
     4681 ttataattaa tattttaata tttttagcgg gaatatatat atgctattta tctagtataa
     4741 gtgtgaagag tggaatatgg attaatttag ctattatgat ggctctagca gggtttatta
     4801 tagaaaataa aactaagtta ttagaatata ttggaggatc tattatacta attattttag
     4861 ctttaagtat attaggtaaa tttgagtata agtcaaatga actatataaa tacgatgtaa
     4921 ataaattagt tttagtaaat aattttcaag gagctaattt tgctaatgaa gagcacgata
     4981 ttcaaagaag tggaagagct ttatattctg aaattaaaaa agagaatatg atattagtag
     5041 ataattctaa tgagaatata aataatttaa ttgaaaataa agagaaaatc atatttaata
     5101 ataaagaaaa taatgaacct gtattttata tgtgtttata taataatatt ccagtatata
     5161 gattagcctt agttaaggat ggccaaatag taaagatata tgagggagta ttaaatcctt
     5221 acatgagaag tacacttatg aagttagaaa gaggaaaagg gcataagaaa aacaaagtat
     5281 gcaatataga gatgcttaaa acaataaatt aagctttgaa ttttgtgtga attaacatta
     5341 aaaaaatcat aatgatatat tgagaggaat atttatatac taaggacaaa tttaattaga
     5401 tttagttctt agtatatttt tttaggcaga atgttgtttt atggagtgat ttcagtggag
     5461 tacaaaaaag agcaatttaa tgaaaaggtt ttaactgatc atattactaa ggaacaatgg
     5521 attgatattt tgaaaaatag taatttaatt ggattaagag atatgaaaat attgcttaat
     5581 ttatatggta gcaatggaca gccccttaag acttctttaa taggagagca tttagagttt
     5641 gaagatatta gtactagaat tcaaaagatg tgtaaaaaaa tagaagaaga gttaggaata
     5701 actttagatg aaaaaagcga aatgggaagt tggaaatata aatttagaca ttggtttatt
     5761 atgctaaatg gatcaaagat atatgacgaa aaggaagaaa aaaattattt cacttggaca
     5821 ttaaaaaagg aacttaaaga agctatagat tctttattgg gaaaagaata gtatgactta
     5881 aagaagtatt gtataaaata agataataaa acttattctt ggcaataaag caagttgttt
     5941 aaaagattag tgttttatta tttgtttttt tatacaatac tttttttaaa attataaatt
     6001 caatttgaag agtttgacag taagagcctt atgaggttaa tatcttatta gcttaacata
     6061 agaaaggaga aagatagtat gaatagaaaa gagataaaaa gtattgctaa gtctaagtta
     6121 aagggaagat ggtttaatat tgtcttatta actttaatca tagcaataat tgaatttgga
     6181 gcaagtgaga ttattggaag aacagaagga ataacttcta atattttaaa catagctaat
     6241 aactttttat taatgccagc cataacggct tcagggatta tatacacaat taaatttgtt
     6301 aagagtaatg aagtagtatc tttaagttat gctattccaa gcattaaaac ttggggaaga
     6361 tttataatga gtatgatagt tactatgatc tttagtattc ctatactttt agttgtgttt
     6421 ttaggaaata tatttttagt gttatctata acatcattac atagtgcaat gcttactggc
     6481 caaatatcta tgaatcctat gataggaatt tggggagtag tatttttaat agttatatta
     6541 gtattaattt tagctgctat agtaggaatt ttattattcc cattaccata tttaatggct
     6601 gaagatacct gtggaatatg ggaagctgtt aagagaagct ttaaaataat gaatggtcat
     6661 aaatgggaac tatttattat gggacttagc ttcttaggat ggttaatatt atcagcatta
     6721 acttttggaa taggattatt atggctttta ccatatattc aagttacctt gagaatatat
     6781 tatctttcaa ttacaggaag agcattagaa ataggaaaat atgaattaaa tcagaatatt
     6841 gaagttaaag aagaagattt cgaatttaga gattagctta aatgcctaag aaatataact
     6901 aattgtattt cttaggcatt ttattttaat tttaacttaa tatattcata tttaattttt
     6961 aatgtaatga atctcataag atagattact ttaaattgaa actaattaaa aatatttact
     7021 taagtggttc cattactctg tctaatatat tattaatttc tgcaattgat gtaccaaagt
     7081 ttgttatagg aactaaagca tcatcacata ttcctattct attcattatt tctgctttat
     7141 taaacatgca tcctccacaa tgaattacaa gggcatattc attaagattc ttaggaaagt
     7201 ccttaccaga acaattatga actattactc caggacaagt ttcttctaac caagtaggta
     7261 gtttttctct tgctatatca ccttttaatt gatggtgagt acaagcttct gctattaaaa
     7321 ctttatcacc tgctttaaga ttttttattg ctttggctcc tgaatagaac ttatctaaat
     7381 cacccttgta tctagccata agtattgaga aggaagtaag aggaacactt ctatctaatt
     7441 ttttattaac ttgtttaaat acttgagaat cagttattac taagtcaggc ttaccgttaa
     7501 agatttttaa tccttcatct aatttatcta atgtaaccat agttggtatt ccaccataat
     7561 ctaatatatc tcttaatact tgaacttgag gaagtataag tcttccctta ggagcttgta
     7621 tatcttgagg agctactaaa agaattttat ctccagcttt gattttatca cctattattg
     7681 ggctttcagt tattgaagag ggtgatacat taacaagttc tttttttaat tcatttatgt
     7741 ttaaattatt ttttgcactt atggcaacac aaggtattcc taataattca ccaatttctt
     7801 ttaaagaatt atttaaatta ctatcaacaa agtaatcttt attagataaa gttgttttat
     7861 caatatcctt taaatcaatt ttagactcaa ttagtttatt aggaactaaa tcacacttgt
     7921 taataactag tatagctggt tttttagcct tttttaattt attaagccat aatatttcct
     7981 tagaaagcat gtctttaaat tctatatttg tttttattgc ctctagtatt tcagaaaggg
     8041 aaagggttat aagagccaca tcagtctttc ctacaacctc ttcagttttt tcgactctaa
     8101 gcttacctat ttctccttca tcatcaaaac cagctgtatc gatgaaaaca acaggtccta
     8161 ggggtaaaag ttccattgcc ttataaacag ggtcagttgt tgttccctta aagtctgata
     8221 ctagagaaat gttttgccct gttagggcat ttattatgct agatttccca gagtttgttt
     8281 taccaaaaag agaaatatga attctacttc ctctaggagt ttcattaaaa ttactcataa
     8341 atttttctac tattgcaaga tataaatagt agaattgcac ctccttgcat taattctttt
     8401 gtgaaaatat tttcaaatat gaataaacct gtggataact tttttattga attttcactt
     8461 tcaaatttta gttaaataat gaaaataagt cttgaaaaaa gtaaaaaaat tacagttata
     8521 aatggctata ttaagccatt tacaggagtt tttatctttt gaaaaaatta cttttttaaa
     8581 tagtaaattt tattaaaaga taatagaaaa atagtataat ttttttgttt ctaaacaagt
     8641 tatccacata tgggtgtgga taactttaag gttattcaca atatatagtt gtgtaatatt
     8701 taagtacata ctaaatattg ataaggaaaa aaa
//
LOCUS       DAEVGI010000102         8679 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEVGI010000102 DAEVGI010000000
VERSION     DAEVGI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.102"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            760..2082
                     /locus_tag="K8M77_001843"
     CDS             760..2082
                     /locus_tag="K8M77_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI7086330.1"
                     /translation="MGKPKLKKEIGLFTATALVVGNMMGSGIFMLPSSLAGVSGPGST
                     IMAWLLTGLGSLILALTFANLGSKIPKTGGTYEYSRLAYGNFMGFMTAWLYWNGSWIG
                     NATIFIVITTYLGEVITSLTNSSIIGFLFCSSILWICTYINIRGTKLAGRVASVITVF
                     KVLLFIFFIVVGLIYFDPSNLTPMFPEGKGVSTIPVAASLTLWAFMGLETASVAGGEI
                     KDPEKNVKRSTILGMLISTVLYILISVVAMGVMSQSELASSTAPISDIIVKVLNLKSL
                     NFLNIAIAISILGTAMGWLLSTARVGYAAGEDGIFPSVFAKVHPKYNTPYVALIIGSI
                     FINIIFLMNFTKGLAGAYSFIVVLATLSYLPIYAISTIAEIILMVKEDKKPSFKKHLG
                     LITRCLIGFAFAIWAIYASGAEIVMYGFILILLGVPIYGYMSIKKHFR"
     gene            2208..2357
                     /locus_tag="K8M77_001844"
     CDS             2208..2357
                     /locus_tag="K8M77_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086331.1"
                     /translation="MAKVEALEEELVELKLKKRNFILANKDTKEIDNLIKKIEEEIMR
                     IKSNN"
     gene            2690..4714
                     /gene="glgB"
                     /locus_tag="K8M77_001845"
     CDS             2690..4714
                     /gene="glgB"
                     /locus_tag="K8M77_001845"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HBI7086332.1"
                     /translation="MTLVEVKDDKLEIKPVRLRKEKYKTQLENKIFNEDDLYLFHEGR
                     NYNAYNFMGAHFTSENRKRGVRFTLWAPRAKNIFLVGDFSNWETKEENKLNRINETGL
                     WSIFIPRLKEGIKYKYYIEQEDGKAVLKADPYGIYSEVRPNTASILCEKTKIRWSDKK
                     WLNKREATNYFESPINIYELHLGSWKRKDEDEFLSYDELSVVLPKYVKEMGYTHVEFM
                     PLNEHPLDASWGYQVTGYYSVTSRYGDIKGLKRLINALHKNDIGVILDWVPGHFCKDE
                     QGLYMFDGTPTYEYEEKWKADNKGWGTFNFDLGKPEVKSFLISNAFYFINEFHIDGLR
                     VDAVSNMLYLNYGRNHGEWIPNIYGGNENLEAIQFIKELNDAIKTYSKGVITIAEEST
                     SWPNVTNDTEYGGLGFDFKWNMGWMNDTLEYNELDPIYRKYHHNKLTFPMMYNHSEKF
                     ILPISHDEVVHGKKSLIDKMQGDYWNKFSNLRAYMAYMYGHPGKKLMFMGCEFGQFIE
                     WREYEELEWKLIDKFDMHRKTHNFFKDLNNFYKNNSELWELDYDGDGFQWIDADNNEQ
                     SIYIFIRKSKNIEKYKIFVCNFTPMVYYDFNIGVPEKGVYREIFNTDKKEYGGSGQVI
                     KGNLFSRKGWCHNQPYTLTIKVPPMAVSVFERIIEENKTEEKIVKEDKYI"
     gene            4760..6190
                     /gene="glgA"
                     /locus_tag="K8M77_001846"
     CDS             4760..6190
                     /gene="glgA"
                     /locus_tag="K8M77_001846"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBI7086333.1"
                     /translation="MKVLFATSEANPFIKTGGLGDVMGALPKELKRKGIDARVILPKY
                     SAIKGELLDKLNFKKWFMVPVGWRNQYCGVYQCEYDEVIYYLLDSEFYFHRNGLYGEG
                     DDGERFAFFDRAVLETLKEIDWCPDIIHCNDWQTGMIPVLHKLEYSKDPFYKDIKTVT
                     SIHNLLFQGNFSADILPELFGYDYEPVRNGSLEFYGGMSFMKGAINYSDRILTVSETY
                     AKEIQTPYFGENLDGLLRERGYALKGIVNGIDYYEFNPSKDSLIAKNFSVETIEDKVL
                     NKLALQKELGLPINQDIPMISIVSRLTNQKGCDLIVNIAHRLLQRNVQLVILGTGDYN
                     YENHFKGLQELYPTKVSANIKFDNGLAHRIYASSDIFLMPSLFEPCGLGQLIALRYGA
                     IPIVRETGGLKDTIHSYNKYTGIGNGFSFTNYNNNDLMHVIELALETYDDKEVWRSLI
                     IQAMDSDNSWNTSAEKYKELYEELIK"
     gene            6220..8655
                     /locus_tag="K8M77_001847"
     CDS             6220..8655
                     /locus_tag="K8M77_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBI7086334.1"
                     /translation="MLSINKESFKRDYKKKFLELHGIELEEGKNFHKYEALGCLVRDY
                     IAEDWVNTKKKYEDSKQKQVYYFSMEFLLGRLLGDALLNLGIRETCREALKELNIDLE
                     ELEEFESDQGLGNGGLGRLAACFLDSMASLNIAGHGCGIRYKYGFFNQKIIDGSQVET
                     PDSWLKNGNVWEVKKPDKSEIVKFGGNIKCENINGRLTFTHVNYEPILAVPYDTPIVG
                     YENNVVNNLRLWSADPVDNEFDFCYFNRGQYLKAIEYRNSIEAISQVLYPDDSMYEGK
                     LLRLKQQYFFVSAGLQSIVRHYKKNGGKIEEFDEYIAIHINDTHPTLAIPELMRILLD
                     EEGLSWESAMRITTNTISYTNHTILSEALEKWPINMFKELLPRIYMIVEEMNERYCAE
                     LWHKYIGQWDKISKMAIIGDGFVRMANLAIVGSHSVNGVAKLHTEILKKKEMSDFYYL
                     YPSKFNNKTNGIAHRRWLLKSNPELTNLLKETIGDSFIKHPTDMENFEKYIDDEYVLK
                     RLGEIKRKNKENLAKVIFDNQGIKVDPNSIFDVQVKRIHAYKRQTLNCLRIMELYNRL
                     LENPNLDIAPRTFIFAGKAAPAYYLAKNTIELINAVANKVNNDKRINDKIKVVFLENY
                     RVSLAERIIPATDLSEQISTTTKEASGTSNMKFMMNGAITIATLDGANIEIRDEVGDD
                     NIVIFGLTENEVLDYYSRGGYSAWDVRNNDNRLIRVTDDLINGVYSKDKDKFRSVYDN
                     LLTYNDEFFVLKDFDSYLKAQDKVDLLYRNNKEWQRKCGVNIAHSGIFSSDRTIAEYA
                     TGIWGSKVIYKNL"
BASE COUNT         3308 a          809 c         1597 g         2965 t
ORIGIN      
        1 aaagctatga actttgttca tagctttttt attttgtaaa taagagtata tttttatata
       61 agatattgat aatagtatat ttaggataaa tacaattact atgtataatt aaatttcata
      121 gtttatctag caaaacctta agatattgtt ggaattttag agtcatcttt aaaagatgac
      181 tctaattttt aatattgatt ataatattat aaaggttaga aagcttataa tataaagaag
      241 aaataaaaag cttaaaataa ttaagcatat atttacataa taattgagca aatatatgta
      301 aactgagttt aaagtatttt tacaaaaaac aatgtataaa tacttatatt ttatagaaaa
      361 taatcatgtt tttatttata attatttatg aaagttaaat tttgattatt gttataagtt
      421 ttatattaag aaaaactaag agctagtaat aagttcaaaa taaagtaata agataatatt
      481 ctataagtag tattttattt atatatactt taaattatac ttattaaaat aattattaaa
      541 ctttagcgat ttaaagttat agagttatta tagtttcttt aattaaattt ttatttatat
      601 taatttatat agggcttata ttataatagc aaaagtaagg attttaaaaa ttttaattaa
      661 ggttatatat gctatataaa attattgaaa tagtataaac taattttcat atagtgaaaa
      721 ttaatatttt ggatttattc gcttttagga ggattaaaaa tgggaaaacc taaattaaaa
      781 aaggaaatag gattgtttac ggctacagct ttagtagttg gtaatatgat gggatctggt
      841 atatttatgc ttccatctag tttagcaggt gtgtctggtc ctggttcaac tataatggcg
      901 tggttattaa cgggattagg ttctttgatt ttggcattaa cgtttgctaa cttaggttca
      961 aaaattccga aaacaggagg aacttatgaa tattctagac tagcttacgg aaattttatg
     1021 ggatttatga cggcgtggct ttattggaat ggatcttgga ttggaaatgc tactattttt
     1081 atagttatta ctacttattt aggtgaagtt ataacaagtt taactaattc atcaataata
     1141 ggtttcttat tctgttcttc tatcttatgg atttgtacat atataaatat aagaggaaca
     1201 aaattagctg gtagagtagc ttcagtaata actgttttta aagttttatt atttatattc
     1261 ttcatagttg ttggattaat ttactttgat ccgtcaaatt taacaccaat gtttccagaa
     1321 ggaaagggtg tttcaactat tcctgtagca gcatctttaa ctttatgggc atttatgggc
     1381 ttagaaactg catctgtagc aggaggagaa ataaaagatc ctgaaaaaaa tgttaagaga
     1441 agtactatat taggtatgtt aattagtact gttttatata tattaataag tgttgtagct
     1501 atgggagtta tgagtcaaag tgaattagct tcaagcactg ctcctatttc tgatataata
     1561 gttaaagttt taaatttaaa gagtttaaat ttcttaaata tagctatagc tattagtatt
     1621 ttaggtacag ctatgggatg gctattatca actgctagag ttggatatgc tgctggagag
     1681 gatggaattt tccctagtgt atttgctaag gtacatccta aatataatac tccatatgta
     1741 gctttgataa taggaagtat atttataaat attatattct taatgaattt tactaagggt
     1801 ctagcaggtg catatagctt tatagttgta ttggctactt taagttattt gccaatatat
     1861 gcgatttcaa ctattgcaga gataatactt atggttaaag aagataagaa accttcattc
     1921 aaaaaacatt taggtttaat aacaagatgt ttaataggtt ttgcttttgc aatttgggca
     1981 atatatgctt caggagctga aatagtaatg tatggattta ttttaatatt actaggagtt
     2041 ccgatttatg ggtatatgag cataaaaaaa cacttccgat aaattgaatt ttgatattta
     2101 acaaatttgt tatgtaatat tagttaattt attatataat aaatataagt tttaaattgg
     2161 gtttatatta tttatggtac agaaatttgc gtggggagtg tattgggatg gcaaaagtag
     2221 aagctttaga agaggaatta gttgaattaa aacttaaaaa aagaaatttc attttagcca
     2281 ataaagatac aaaagagatt gataatctta taaaaaaaat agaagaagaa attatgagaa
     2341 ttaaatcaaa taattgatat tcagtagcta tgaaagagaa attactcttt catagctttt
     2401 ttttaataaa aagaaagtat tctcaaagaa acttttaaat tgagatcagt caaatatgcc
     2461 ttcatagtga tcaatatgtt cttatttaag aaaaatatta ttttgatata taaaatttat
     2521 ttaacgatat gaaattttta aatattgttt tggtaaaaaa atacatttaa tatattaatg
     2581 agtttacttt ttataagatt attttagaat aatttaatta aataattaga tttgaatttg
     2641 tataaataat atttttattt atatcttaag aagggtaagg agatttgtta tgactttagt
     2701 agaggtaaaa gatgataaat tagaaataaa acctgttagg ttgagaaaag aaaaatataa
     2761 aacacaatta gaaaataaaa tttttaatga agatgattta tatttatttc atgaagggcg
     2821 aaattataat gcttataatt ttatgggagc tcattttact agtgaaaata gaaaacgagg
     2881 agtaagattt accctttggg ctccaagggc aaagaatatt tttttagttg gagatttttc
     2941 aaattgggag acaaaagaag aaaataaact taatagaata aatgaaacag ggctttggag
     3001 catttttata cctagattaa aagagggaat taagtataag tattatattg aacaagaaga
     3061 tggaaaagca gtattaaagg ctgatcctta tggaatttat tcagaggtaa gacctaatac
     3121 tgcttctatt ttatgcgaaa aaactaaaat aagatggtca gataaaaagt ggttaaataa
     3181 aagagaagca actaattatt ttgaaagtcc tattaatata tatgaattac acttaggatc
     3241 atggaaaaga aaagatgagg atgagttttt atcttatgat gaattaagtg tggttttacc
     3301 taagtatgtt aaggaaatgg gatatactca tgttgaattt atgccattaa atgagcatcc
     3361 attagatgcc tcttgggggt atcaggttac aggatattat tcagtaacaa gtagatatgg
     3421 agatattaag ggattaaaaa ggttaataaa tgcccttcat aaaaatgata taggagttat
     3481 tttagattgg gtaccaggtc atttttgtaa ggatgaacaa ggattatata tgtttgatgg
     3541 tacaccaact tatgaatatg aggaaaagtg gaaagctgat aataaaggat ggggaacttt
     3601 taattttgat ttgggaaaac cagaagtaaa aagcttttta atttctaatg ctttttactt
     3661 tataaatgaa tttcatatag atggattaag ggttgatgct gtttctaata tgctttactt
     3721 aaattatgga agaaatcatg gagaatggat tcctaatata tatggaggaa atgaaaattt
     3781 agaagctatt caatttataa aagagcttaa tgatgctata aaaacttata gcaaaggggt
     3841 aattacaatt gcagaagaat caacatcttg gcctaatgtg actaatgata ctgaatatgg
     3901 cggcttagga tttgatttta aatggaatat gggttggatg aatgacactt tagaatataa
     3961 tgagttagat cctatatata gaaagtatca tcataataaa ttaactttcc ctatgatgta
     4021 caatcattca gagaaattta tattacctat atctcatgat gaagttgttc atgggaaaaa
     4081 atctcttata gataaaatgc agggagatta ttggaataag ttttctaatt tgagagcgta
     4141 tatggcatat atgtatggtc atccagggaa aaaacttatg tttatgggat gcgaatttgg
     4201 acagttcata gagtggagag aatatgaaga gcttgagtgg aagttaattg ataagtttga
     4261 tatgcataga aaaactcata atttctttaa ggatttaaat aacttttata aaaataattc
     4321 tgagctttgg gaattagatt atgatggaga tggatttcaa tggattgatg ctgataataa
     4381 tgaacaaagc atatatattt ttataagaaa aagtaaaaat atagaaaagt ataaaatatt
     4441 tgtatgcaat tttactccaa tggtttatta tgattttaat ataggagttc ctgaaaaggg
     4501 agtttataga gaaattttca atacagataa gaaagaatat ggtggttctg gacaagttat
     4561 taaagggaat ttattctcaa gaaaaggttg gtgccataat cagccatata ctttaaccat
     4621 aaaggttccg ccaatggcag tttctgtttt tgaaagaata attgaagaaa ataaaacaga
     4681 agaaaagata gtaaaagaag ataaatatat ttaataaaag ataaaaggga agatatttta
     4741 tttggagggg tttaaattga taaaagtttt atttgcaaca tcagaggcaa atccatttat
     4801 aaaaactggg ggactaggtg atgtaatggg agcattacct aaggagctta aaagaaaggg
     4861 aatagatgct agagtaattt tgcctaaata tagtgctata aaaggagaac ttttagataa
     4921 gcttaatttt aaaaaatggt ttatggttcc tgttggttgg agaaatcaat attgtggagt
     4981 atatcagtgt gaatatgatg aagttatata ttatttatta gatagtgagt tttatttcca
     5041 cagaaatggt ttatatggag aaggtgatga tggagagaga tttgctttct ttgatagagc
     5101 tgttttagaa actttaaaag agattgattg gtgtccagat attattcatt gtaatgattg
     5161 gcaaacagga atgataccag tacttcataa attagagtat tctaaagatc ctttttataa
     5221 ggatattaag actgtgactt caattcataa tcttttattc caaggtaatt tttcagcaga
     5281 tattttacct gaactttttg gttatgatta tgaaccagtg agaaatggaa gtttagaatt
     5341 ttatggtgga atgagtttta tgaagggagc tataaattat agcgatagga ttttaactgt
     5401 aagtgaaact tatgccaagg aaattcaaac tccttacttt ggagaaaatt tagatggctt
     5461 attaagagaa agaggatatg cactaaaagg aatagtaaat ggtatagatt attatgaatt
     5521 taatcctagt aaggatagtt taatagctaa aaatttctca gtggaaacta tagaagataa
     5581 agtgttaaat aaattagctc ttcaaaagga attagggtta cctataaatc aagatatacc
     5641 tatgataagc attgtatcta ggcttacaaa tcaaaaggga tgcgacttaa tagttaatat
     5701 agcacataga ttgcttcaaa gaaatgtgca attagttata ttaggaacag gtgattataa
     5761 ttatgaaaat cattttaagg gattgcaaga gctttatcca actaaggtat ctgccaatat
     5821 aaagtttgat aatggattag ctcatagaat atatgcatct tcagatattt tcttaatgcc
     5881 atcattattt gagccttgtg gactaggaca acttatagca ttaagatatg gagctattcc
     5941 aatagttaga gaaacaggtg gattaaagga tacaattcat tcatataata agtatactgg
     6001 aataggaaat ggctttagtt tcacaaatta taataataat gatttaatgc atgttattga
     6061 attagcttta gaaacatatg atgataaaga ggtttggaga tcattaatta tacaagctat
     6121 ggattctgat aatagttgga atacttcagc agaaaaatat aaggaattat atgaagaatt
     6181 aataaagtag ttttaatcaa gtttgtagga ggcgagtaga tgctatctat aaataaggag
     6241 agttttaaga gagattataa aaaaaagttc ttagaacttc atggtataga acttgaagag
     6301 ggaaaaaatt ttcataagta tgaggctctt ggatgtttag ttagagatta tattgcagaa
     6361 gactgggtaa atactaaaaa gaaatatgag gattcaaagc agaaacaagt ttattatttc
     6421 tctatggagt ttttattagg aagactttta ggagatgcct tactaaactt aggtataaga
     6481 gaaacatgta gggaagcttt aaaggagtta aatattgact tagaagaact agaagagttt
     6541 gaaagtgatc aggggttagg taatggtgga ttaggtagat tagcagcatg ctttctagat
     6601 tcaatggcat ctttaaatat agctggacat ggatgtggca taagatataa atatggattt
     6661 ttcaatcaaa aaataattga tggatcacag gtagaaactc cagatagttg gcttaagaac
     6721 ggaaatgtat gggaagttaa aaagcctgat aaatcagaaa ttgttaagtt tggtggaaat
     6781 attaagtgtg aaaatataaa tggtagatta acttttaccc atgtaaatta tgagccaatt
     6841 ttagcagttc cttatgatac tcctattgtt ggatatgaaa ataatgtagt aaataattta
     6901 aggttatgga gtgcagaccc agtagataat gaattcgatt tttgttattt caatagaggt
     6961 caatatttaa aggctataga atacagaaat tctattgaag ccatatcaca agtattatat
     7021 cctgatgact caatgtatga agggaaactt cttagattaa aacagcaata cttctttgta
     7081 tcagcaggac ttcagagtat agttagacat tataagaaaa atggtggaaa aatagaagag
     7141 tttgatgaat atattgctat tcatataaat gacacccatc caaccttagc aataccagag
     7201 cttatgagaa ttttattaga tgaagagggt ctttcatggg aaagtgccat gagaataact
     7261 acaaacacaa tatcatatac aaatcatact attttatccg aagccttaga gaaatggcca
     7321 ataaatatgt ttaaagagct tcttcctaga atatatatga tagttgaaga gatgaatgaa
     7381 agatactgtg ctgaactttg gcataagtat attggtcaat gggacaagat ttctaagatg
     7441 gctataatag gagatggctt tgttagaatg gctaatcttg caatagtagg cagtcatagt
     7501 gtaaatggtg tagcaaaatt gcatacagaa atacttaaga aaaaagagat gagtgatttt
     7561 tattatcttt atccaagtaa atttaataat aaaactaatg gaatagcaca tagaagatgg
     7621 cttctaaaaa gcaatcctga acttactaat cttttaaaag aaacaatagg agacagcttt
     7681 ataaaacatc ctacagatat ggagaacttt gagaagtata ttgatgatga gtatgtttta
     7741 aaaagattag gagaaataaa gaggaaaaat aaagagaatt tagctaaggt aatttttgat
     7801 aatcagggaa ttaaagttga tccaaattca atttttgatg ttcaagttaa gagaatacat
     7861 gcatataagc gtcagacttt aaattgtttg aggataatgg agctttataa tagattatta
     7921 gaaaatccaa atttagacat agccccaaga acctttatat ttgcaggtaa agctgcccct
     7981 gcatactatt tagctaagaa tactattgaa ttaataaatg ctgttgcaaa taaagtaaat
     8041 aatgataaaa gaattaatga taaaatcaaa gtggttttct tagaaaatta tagagtttct
     8101 cttgcagaaa ggataattcc agctacagac ttaagtgaac aaatctcaac tacaactaag
     8161 gaagcttcag gtacttctaa tatgaagttt atgatgaatg gtgctattac tatagctacc
     8221 ttagatggag ctaatataga aataagggat gaagttggag atgacaatat tgttattttt
     8281 ggattaacag aaaatgaagt tctcgattat tactcaagag gtggatattc agcttgggat
     8341 gtaagaaata atgataatag gttaattaga gttacagacg atttaataaa tggagtttac
     8401 tctaaggata aagataaatt tagaagtgta tatgataatt tattgaccta taatgatgaa
     8461 ttttttgtac taaaggattt tgattcatat ttgaaagctc aagataaggt agatttgctt
     8521 tatagaaata ataaagagtg gcaaagaaaa tgtggagtaa atatagcaca ttcaggtata
     8581 ttttcttcag atagaaccat agctgagtat gctacaggaa tatggggttc aaaagttata
     8641 tataaaaacc tatagaaatt tataacaaag tataatcca
//
LOCUS       DAEVGI010000103         8543 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEVGI010000103 DAEVGI010000000
VERSION     DAEVGI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8543
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.103"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(16..801)
                     /locus_tag="K8M77_001848"
     CDS             complement(16..801)
                     /locus_tag="K8M77_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBI7086335.1"
                     /translation="MDKYFSIGEVSKITGLSIDRLRNYDKIGLLKPSYIDPKSGYRYY
                     SENKFRKLRIIKYLRKIGTPLKEIDLILNKNIDSQEFAKFLELKIRDIEKEIESLNMI
                     KEDISEIKHTFECNFKLSNINYIHKKYIDERYVVKKSLNKNINFVVSHREQVFSKFKS
                     EINTLEPPRSLEKGLYLHSLEDLENNNTEVYMLLKDYENLHNFIIPSGNYLCLSYKEN
                     ERDKAIFKLKSYIEENHIKIKGPILNLVLTTLPKEEFQFQILI"
     gene            914..2734
                     /locus_tag="K8M77_001849"
     CDS             914..2734
                     /locus_tag="K8M77_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7086336.1"
                     /translation="MIIKQMNIKRFKLPLIFAVLSVVLMGLYKTIFGVENTIIGLIIS
                     MASYAFLRLDLTSYPIYKSMIFLILNLFLAISAYISAINPFVGLIINFLILFTVSFIY
                     TTEFKNVISYIFLLLYVYMLEYPISLDELPRRLVAMGVGIFIIIGIHILFNRRNFKKN
                     SNNIIIRSIRNIQKEICHIINESYKEKENIYIDSELRKLLILIEVRNNNKFIENYKDD
                     IYFNIVLILERINSIINKVGKINNKSKYVIDYLNRLNHDLENITLFLERKVDCINEEK
                     DDLNKPSMINNFTDKEYAFLGECTELIRLLEKNINNLYEYRREKSRKRIKVKFNLKEL
                     LIGNSSLKVKDLRVAYSLKLAIAVSLIMFIVDLFKIHQGRWIVTSVYVVIQPYEEETL
                     TKAIKRFKGTIIGVIIYISIFTFFPHIIPLELLLLILMFLYFTQKDYDKKVVCTALMA
                     LSFGLSRSTVGYLAFYRFFFVIIGIVIALGVNKLIFPQSIKNSIYDLKERYLELTSKL
                     LCELKSILYEEGYNGNTVKLLLDCNLIESKLIENKLIAENLELKDLVDKQSIILSKIR
                     CLVLFINYSNWGISSKHINVDKNLLNVIFNKIEEELREIY"
     gene            3038..3547
                     /locus_tag="K8M77_001850"
     CDS             3038..3547
                     /locus_tag="K8M77_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7086337.1"
                     /translation="MDFITITNYFAEYGLLFLFVIILLEYMNLPGLPAGIIMPAVGVL
                     VSRSEMSLITALLVSVIAGLIGSWILYCIGRFGGELILQKYLNKFPSHESFVNRKIDY
                     LREKGYTGILISKLIPMARTVISIPAGVLKLNFLKYSAYATLGIFIWNTVLISSGYFL
                     GEELLKFLG"
     gene            3864..4568
                     /locus_tag="K8M77_001851"
     CDS             3864..4568
                     /locus_tag="K8M77_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7086338.1"
                     /translation="MEKYNVLVVEDEKEIAEAIEIYLKNQGYNVFKGSNGLEGLEIVE
                     KEEIHLAVVDIMMPKMDGATMVMKIRESYDFPIIMLSAKSEDMDKILGLNIGADDYVT
                     KPFNPLELLARVNSQLRRYSKYLNMVKKDEKKTNSYAIGGLEVNSDRKEVILDGDVVK
                     VTPIEFKILQLLIKSPGRVFSAEEIYERVWNENAVNTDTVMVHVRNIREKIEIDPKNP
                     KYLKVVWGVGYKIEKQ"
     gene            4543..6774
                     /locus_tag="K8M77_001852"
     CDS             4543..6774
                     /locus_tag="K8M77_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7086339.1"
                     /translation="MAIKSKSNKENLQVNKENRTNKTKWIFTIITLILIGIISVCLLL
                     SYPIIKKNTPNYITSYGLFNNYNFREKIIDTNYGIYYDYLEKKAEENEKEMNPMEDLF
                     TLKNENRYEDKESYEYSLKEDTRLLNERVKSLINGLHDLGNLRVYAMDEEGNTVYETK
                     GDQNWLLEALAKGSYIENGDLENYYRFYMVLKYNNRGKVSLEKIYGEDSHRVSNLLLD
                     MDSQYFAYQNEYEAKPIENMTYVYAVPKTLEYQDNLYYSEMDNKIMALGSASAPFIMI
                     GMIVTCLLALLIPFKYTREVLGFNKFLKIPFEINFIIASIMLAFTIESGRLIIIPTLQ
                     GGLSEALKMTSLPHLDIVTNIINVLYWMVIYGFVFFEVIYVKYIFNIGFKNYIRKHLL
                     VYKLKDIIFVILRKLIKYIKGIDLNKNSDKFLLIILGINFVIVSILCSVWFFGIFGVV
                     IYSGILFYFGRKYIDKWKKDYNKILEKTKEISNGNLDSKDDEDCGFFNPMRDELNNIQ
                     KGFKAAVEEEVKSQRMKTELISNVSHDLKTPLTSIIAYVDLLKGEDDEEKRKAYLETL
                     ERKSQRLKDLIEDLFEVSKATTGNINLNIMDVDISYLMKQVVLELDDKINEAGLDIRL
                     SLPEEKVILPLDGQRTYRVFENLIINVVKYALPNSRVYIDIKNRIENVDVIIKNISAD
                     EITFNINEISDRFVRGDKSRNTEGSGLGLAIVKSFVELQGGKFHIEVDGDLFKAIISF
                     KKN"
     gene            7077..8114
                     /locus_tag="K8M77_001853"
     CDS             7077..8114
                     /locus_tag="K8M77_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7086340.1"
                     /translation="MIESIKKNLKPKILISNLILIIGIVIFVTLYGAAFGGNNSLIGV
                     CAITAMLMFVDVHLSLKLNEAIITTVLSFVLMGVSSQIASINPFLGFVINFISIFVVS
                     YLVTNVTETKAYLPFILCYVFVEGTPITWSELPRRLIALFVGGALISLVYYFSHRKKD
                     DSDHMNISEMIKTMNKDTLQFNFSLRMALAVSIAMLLGSIFGFQKSMWISITAMSITQ
                     PHFDDTKTRVKERFFGTLIGAAIFLLIFVYLVPQQFSSIVLLILSYIYTFIKEYKIKM
                     IFITMSSLGAAMILFQPGVSIPMRIAFIVVGIVIALVVNKVIYGKLKLEESNEELDET
                     IKDIDKVNDEF"
BASE COUNT         3399 a          788 c         1335 g         3021 t
ORIGIN      
        1 aatattctta ttcttttaaa ttagaatttg aaattgaaat tcttcctttg gaagtgttgt
       61 tagtactaaa tttaaaattg gtcccttaat tttaatatga ttttcttcaa tataggattt
      121 taatttaaaa atagctttat cacgttcatt ttctttatat gaaaggcata aatagttacc
      181 actaggtata ataaaattat gtaggttttc ataatctttt agaagcatat acacctctgt
      241 attattattt tctaagtctt ctaagctatg taaatagagt cctttttcta gagaacgagg
      301 aggttctaaa gtatttattt ctgatttaaa ctttgaaaat acttgttctc tatgggaaac
      361 aacaaaattt atatttttat ttaggctttt ctttactaca tatctttcat caatatactt
      421 tttatgaata taatttatat tgcttaattt aaagttacat tcaaaggtat gtttaatttc
      481 acttatatct tcttttatca tatttaaact ttcaatttct ttttcaatat cccttatttt
      541 aagttcaaga aacttcgcaa attcctgaga atctatattt ttatttagta ttaaatcaat
      601 ttcttttaag ggagtaccaa tttttctaag atattttata atccttaatt ttctaaattt
      661 attttctgaa taatatctat atccactttt aggatcaata taagaaggct ttaataatcc
      721 tattttgtca tagtttctta gtctatctat ggataaacca gtaatttttg agacttcacc
      781 aattgaaaaa tatttatcca ttaaaacaat ctctcacttt ctaaaaaagt ctttactcta
      841 gtattatact agactttata atacttatta ttataaattt tattaattaa agataagaag
      901 agtgaaaatt acaatgataa ttaaacaaat gaatataaaa agatttaaac tacctttaat
      961 atttgcagtt ttatctgtag tattaatggg attatacaaa acaatatttg gagtggaaaa
     1021 cactattata ggattaataa tatcaatggc gtcctatgct tttctaagat tagatttaac
     1081 ttcatatcca atttataagt ctatgatatt tctaatatta aacttatttt tagccataag
     1141 tgcttatata tctgccataa atccatttgt tggattaata ataaactttt taatactttt
     1201 tacagtatca tttatatata caacggaatt taaaaatgtt atttcttata tattcctatt
     1261 gttatatgtg tatatgttgg aatatcctat aagtttagat gagttgccaa gaaggttagt
     1321 ggctatggga gtaggtattt tcattattat tggaattcac atattattta atagaagaaa
     1381 tttcaaaaag aattcaaata atataatcat aagatctata agaaatattc aaaaagaaat
     1441 ctgtcatata ataaatgaaa gctataagga aaaggaaaat atctatatag atagcgaatt
     1501 aagaaaatta ttaattttga ttgaagtaag aaataataat aaatttatag aaaattataa
     1561 ggatgatatt tattttaata ttgtacttat attagaaaga ataaattcaa taatcaataa
     1621 agttggtaaa attaataata aatctaaata cgtaatagac tatttaaata gattaaatca
     1681 tgatttggaa aacataactt tatttttaga aagaaaagtg gattgtatta atgaggaaaa
     1741 ggatgattta aataagcctt ctatgataaa taattttact gataaagaat atgctttttt
     1801 aggggaatgc actgaactta taagactttt agagaaaaat ataaataatt tatatgaata
     1861 taggagggag aaatcaagga agagaattaa agtaaaattt aatcttaagg aactactaat
     1921 aggaaatagt tctttaaaag tgaaagattt aagggtagcc tattctttaa aactagccat
     1981 agcagtttcc ctaataatgt ttatagtaga tttatttaaa atacatcagg gaagatggat
     2041 tgttactagt gtttatgtgg ttatacagcc ctatgaagaa gaaaccttaa caaaagcaat
     2101 aaaaagattc aaagggacaa taataggagt gataatctat atttctatat ttacattttt
     2161 cccacatatt attcctttag aattgcttct attaatttta atgtttcttt actttactca
     2221 aaaggattat gataaaaagg ttgtatgcac agcacttatg gctctaagct ttggattatc
     2281 tagaagtaca gttggatact tagcttttta tagatttttc tttgtaataa tagggatagt
     2341 aatagcttta ggagttaata agcttatttt tccacagagc ataaaaaatt ctatatacga
     2401 tttaaaggaa aggtatttag aattaacaag taaacttctt tgtgagttaa agagcatatt
     2461 atatgaagag ggatataatg gaaatacagt taaacttcta ttagattgta atcttattga
     2521 gtctaagtta atagaaaata aattaattgc tgaaaattta gaacttaaag acttagtaga
     2581 taaacagagc ataattttga gtaagataag gtgtcttgta ttatttataa attactcaaa
     2641 ttggggaatt tcatcaaaac acattaatgt agataaaaat ttattaaatg taatttttaa
     2701 taaaatagag gaagaactaa gggagattta ttaggaataa aagatcacat aaaatgatgt
     2761 tacttaaaag tttagaagtt aagaattaag ttaaattaag attactaact aaaatcaaaa
     2821 aaatattaag aaactcttta gaaaatcttt agatttatct gaggagtttc tttattttta
     2881 aagtttatta taagagtaac aaatgagaag aaagcaagta acaatttaga taatagaaat
     2941 gctttttaga attaagttag tttaaaagca ttttagaatt aacaattaat aaaaatatat
     3001 aggtcaaata aaattttaaa attgaaaggt aaggggtatg gattttataa ctataacaaa
     3061 ctattttgca gagtacgggc ttttattttt atttgtgatt attcttttag aatatatgaa
     3121 tttaccaggg cttcctgcag ggataataat gccagcagtg ggcgtgttag tttcaagaag
     3181 tgaaatgagt cttataacag cacttttggt atctgtaata gcaggtctaa taggaagttg
     3241 gatactttat tgcataggaa gatttggagg agaattaatt cttcaaaaat atctaaataa
     3301 atttccaagt cacgaaagct ttgtaaatag aaaaattgat tatttaagag aaaagggtta
     3361 tacaggaatt ttgataagca aactaattcc aatggctagg acagtaatat caataccagc
     3421 aggagttttg aaattaaatt ttcttaagta tagtgcttat gcaactttag gaatttttat
     3481 atggaataca gttttaatat catcaggata tttcttagga gaggaattat taaaattttt
     3541 aggatagaaa taaggaggtg ttttttggaa atatagacaa tgaggtatta acaaacttta
     3601 aagaatataa ggaaatagtt aaggaaaggc tatgtttcta acagtgaaaa agctttctat
     3661 gatattatta tattgaaatt attggataaa atttaaagga ttagagaaat attttagttt
     3721 aagcttataa aattagatag tctttaagag agagctaaaa tcaacactaa ttaacttata
     3781 gctaaattta aatatagaat taacctaagt ttttatatgg tatctaataa aaatatagta
     3841 ataaaaaagg gagtaataaa gagatggaaa agtataatgt tttagttgtt gaagacgaaa
     3901 aggaaatagc agaggctatt gaaatatatc taaaaaatca aggatacaat gtatttaaag
     3961 gaagtaatgg tttagaagga ttagaaatag ttgaaaaaga agagattcat ctagctgttg
     4021 ttgatataat gatgcctaaa atggatggag caacaatggt aatgaaaatt agagaaagtt
     4081 atgactttcc tataataatg ttatcagcaa aatcagagga catggataaa attttaggac
     4141 ttaatattgg agccgatgat tatgtaacaa aaccatttaa tccactagag cttcttgcaa
     4201 gggttaattc tcaattaaga agatattcta aatacttaaa catggtaaaa aaggatgaga
     4261 agaaaacaaa ttcttatgcc ataggtggat tagaagttaa tagtgataga aaagaagtaa
     4321 tattagatgg agatgtagta aaagtaactc ctatagaatt taaaatactt caattactta
     4381 ttaaaagtcc aggaagagtt ttttcagcag aagaaatata tgaaagagta tggaatgaaa
     4441 atgcagtaaa cacagacact gtaatggtcc atgtaagaaa tataagagaa aaaatagaga
     4501 tagatccaaa aaatccaaaa tatttaaagg tggtgtgggg agttggctat aaaatcgaaa
     4561 agcaataagg aaaacttaca agttaataag gaaaatagaa ctaataaaac aaagtggatt
     4621 ttcacaataa taaccttaat attaatagga attatatctg tatgtttatt attatcatat
     4681 cctataataa agaaaaatac tccaaactat attacaagtt atgggttatt taataattat
     4741 aattttaggg aaaaaataat tgatacaaac tatggaattt actatgatta tttagaaaaa
     4801 aaagctgagg aaaatgaaaa agaaatgaat cccatggagg atctatttac tttaaaaaat
     4861 gaaaatagat atgaagataa agagtcttat gaatatagtt taaaagaaga tactagatta
     4921 ttaaatgaac gtgttaaaag tcttattaat ggattacatg atttaggaaa tctaagagta
     4981 tatgctatgg atgaagaagg aaatacagta tatgaaacca aaggagatca aaattggttg
     5041 cttgaagctt tagcaaaggg ttcatatatt gaaaatggtg atttagaaaa ttattataga
     5101 ttttatatgg tacttaagta taacaataga ggaaaggttt ctttagaaaa aatttatgga
     5161 gaggatagcc atagggtaag caatttatta ttagatatgg attcacagta ttttgcatat
     5221 caaaatgagt atgaagcaaa accaatagag aatatgacct atgtgtatgc agtaccaaaa
     5281 actttagaat atcaagataa cttatattat agtgaaatgg ataataagat tatggcactt
     5341 gggagtgcta gcgctccttt tatcatgatt ggaatgatag tcacttgttt acttgcttta
     5401 cttattccat ttaaatatac aagagaggtt ttaggtttta ataagttttt aaagattcct
     5461 tttgagatta actttataat agcttcaatt atgctagcat tcacaattga aagtggaaga
     5521 cttataataa ttccaacact tcaaggagga ttaagtgaag ccctaaaaat gactagctta
     5581 ccacatttag atatagttac taatataatt aatgtgcttt attggatggt catatatggc
     5641 tttgtattct ttgaagtaat ctatgtaaaa tatatattta atattggatt taaaaattat
     5701 ataagaaagc atttattagt ttataaatta aaagacatta tatttgttat cttaagaaaa
     5761 ttaataaaat atattaaagg aatagattta aataaaaata gtgataagtt tcttttaata
     5821 attttaggaa taaactttgt cattgtctca atcctttgtt cagtttggtt ctttggaata
     5881 tttggagtag taatatattc aggaatttta ttctactttg gtagaaaata tatagataag
     5941 tggaaaaagg actataacaa aattttagaa aagaccaagg aaatatcaaa tggaaactta
     6001 gactctaagg atgatgagga ttgtggcttt tttaatccaa tgagagatga attaaataat
     6061 atccaaaagg ggtttaaagc agcagttgaa gaagaagtta aaagtcaaag aatgaaaaca
     6121 gagcttatat caaatgtttc tcatgattta aaaactccat taacatcaat aattgcttat
     6181 gttgatctat taaaaggaga agatgatgag gaaaaaagga aagcttactt agaaacttta
     6241 gagagaaaat cacaaaggtt aaaagactta atagaagatt tatttgaagt aagtaaagct
     6301 actactggaa atattaattt aaatataatg gatgtggata tttcatatct tatgaaacaa
     6361 gtagttttag aattagatga taaaataaat gaagctggtt tagatataag actttcattg
     6421 ccagaagaaa aagtaatatt accactagat ggacaaagaa cttacagggt atttgaaaat
     6481 ttaataatta atgtggtgaa atatgctctt cctaattcaa gagtgtatat agatattaag
     6541 aatagaatag aaaatgttga tgtgatcata aaaaatattt cagcagatga aataacattt
     6601 aatattaatg aaatttcaga tagatttgta agaggagata aatctcgtaa cacagagggt
     6661 tcaggattag ggcttgcaat agttaagagc ttcgttgaac ttcaaggtgg aaaattccat
     6721 atagaagtag atggtgattt attcaaagca ataataagct ttaagaaaaa ttaatttaaa
     6781 ttctaaagct catctagata ttctcttcat attgtttgta aggtttatac ttattttaat
     6841 atagcttttg taaataatat gttattttag aattataagt cttattttta aaatctataa
     6901 ttaaaaaata tataattact agatatatta ataaagtaag aaagaagggt tcaaagtaat
     6961 aaaaaaatgc tttgaactct aattttttgt ataaaaaact tatttcacta aatttcttaa
     7021 atatggtata atgctcgaca gaagaaatga ctagatggaa aagaggaata gagagaatga
     7081 tagaaagtat aaagaagaat ttaaaaccta agattcttat aagtaattta atattgataa
     7141 ttggtatagt tatttttgtt accttatatg gagctgcgtt tggaggtaat aatagtctta
     7201 taggggtatg tgccataact gcaatgctta tgtttgtaga tgttcattta agtttaaaat
     7261 taaatgaggc tattataact acagtgttat cctttgttct tatgggagtt tcaagtcaaa
     7321 tagcttcaat aaacccattt ttaggatttg taataaattt cataagtata tttgtagttt
     7381 catatttggt tactaatgtt acagaaacaa aagcatattt accttttata ctttgttatg
     7441 tatttgtaga aggaactcct attacatgga gtgaattgcc aagaagactt atagcattat
     7501 ttgttggagg agcattaata tctttagttt attattttag tcatagaaaa aaagatgact
     7561 ctgatcatat gaatataagt gaaatgataa aaactatgaa taaagatacc cttcaattta
     7621 acttttcact gagaatggca cttgctgtat ctatagccat gttacttgga agtatatttg
     7681 gttttcaaaa atcaatgtgg ataagtatta ctgcaatgtc aataactcaa ccacattttg
     7741 atgatactaa aactagggtt aaggaaagat tctttggtac tttgataggt gcagcaatat
     7801 ttttattaat atttgtttat cttgtaccac aacagtttag tagtatagtt ttacttatcc
     7861 taagttatat ttacacattc atcaaagaat ataaaataaa gatgatattc ataaccatga
     7921 gttctttagg agctgcaatg attttattcc aaccaggagt ttcaatacct atgagaatag
     7981 cttttatagt agttgggata gttatagctt tagtagtaaa taaagttatt tatggaaaat
     8041 taaaattaga ggaaagcaat gaagaattag atgagactat taaagatata gataaagtaa
     8101 atgatgaatt ttagagtaaa tatacgatgc tataattaga gctttataga tataattaaa
     8161 gagtataaag cttaacttta gagaaagtat atcgtattga ttaaagtata taaaattagc
     8221 ttttaagaaa agcttattaa atatgcttaa gctaaatatc cataataatt aaaaatatat
     8281 attgaattat taagaggctt gtacttaaag ttactcatta ataaaattta tagattctta
     8341 aagagagata tatgactttt tatatttcaa aataatatag aaggttttgt attaattgat
     8401 ttaaggaaga ggtttataag ttttatagtg agttttggta caagccattt ttattagatt
     8461 tttaataata tatataagat ttaaataaag ttttaaataa taataataga gctttaaaaa
     8521 ttttttagat atatatttaa att
//
LOCUS       DAEVGI010000104         8320 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEVGI010000104 DAEVGI010000000
VERSION     DAEVGI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.104"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            90..689
                     /locus_tag="K8M77_001854"
     CDS             90..689
                     /locus_tag="K8M77_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBI7086341.1"
                     /translation="MDINNFNSEQIKFLAEEMSKNSMVSYDDLPKYDLFLSQVIDYLN
                     DKFTEDKFTNNIVQNYIKSEVISKPEDGKKRGYTKVHLAQLVLLSYMRPLLKTDEIKK
                     VFALAFNNINDRSDDIISWETAYKMFGEVQEESIKRFLEVPLVDNDKIEEIIQVSDIK
                     EEDRDCIVTFLTVMTLIAQASVVKKVVQKIVNGYHKEEK"
     gene            complement(726..1601)
                     /locus_tag="K8M77_001855"
                     /pseudo
     CDS             complement(726..1601)
                     /locus_tag="K8M77_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465640.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(1712..2734)
                     /locus_tag="K8M77_001856"
     CDS             complement(1712..2734)
                     /locus_tag="K8M77_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBI7086342.1"
                     /translation="MSQGKHAKKNDSNSQGNTPKKKSKVKIIVLTLFFLLLIGIGLGA
                     TYVYSTLNKMDIKKIAQDDKSLGIDESNKDLFQDGILNIALFGVDSRDHNNVGRSDSI
                     IIATIDTKHDKIKLTSLMRDSYVEVDGHGKTKLTHAYAYGGPTLALKTINENFGLDIK
                     DYVTVNFDNLAEIIDDLGGVPINIKPYEVKEVNNYAKNVAEIAGREYTPVSEGEQVLN
                     GAQAVGYSRIRYVGDGDYERTERQRNVLDAIIKKLSTLKPSEYPETIKKFLPYVETNL
                     TPSKILSIAKSVASTGIPPVENMRFPLNGYCKGEMIDGVWYLTFDEAKTKEQIQNYIY
                     KDVNPK"
     gene            2910..3761
                     /locus_tag="K8M77_001857"
     CDS             2910..3761
                     /locus_tag="K8M77_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBI7086343.1"
                     /translation="MKKKSKGFLRKTVIFLVMFIKKIGDDDIFALGAQLAYYMVLSFI
                     PFLMFLMNLVGFSHLNSDTILNLLSNVMPTEAFNLIQSTVIEIVDKEQTGLLWISIAL
                     AIWVSSSGFKAVIKGLNKAYGVKETRSYIKLKLISMIYTILLALIVIATLFLFVFGDV
                     IGDFFIKVLEHPEVIYYIWNMLRYVVVILIMVLFFMFLYNATPCVRLGWLEVIPGAVI
                     TTLGWISISYIFAYYVNNFSNYSRLYGSLGAVFMFMTWMFITSMILILGGEINAVLAE
                     KNRLKDL"
     gene            complement(3822..4808)
                     /gene="galE"
                     /locus_tag="K8M77_001858"
     CDS             complement(3822..4808)
                     /gene="galE"
                     /locus_tag="K8M77_001858"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI7086344.1"
                     /translation="MAILVCGGAGYIGSHMVAELIENNKEVVILDNFEKGHEDAILGG
                     KLYKGDLRDRKILDKIFTENNIEAVIDFAAYSLVGESMTEPLKYFNNNVSGTISLLEA
                     MRDHNVKYIVFSSTAATYGEPENIPILETDKNLPTNAYGESKLLVEKILKWCDTAYGI
                     KYTALRYFNAAGAHVNGKIGEDHAPETHLIPLILQVALGKRDKIMMFGDDYDTKDGTC
                     VRDYIHVSDLASAHSLALERLMNGGESRIYNLGNGTGFTVKEVVEVARKVTGHQIPAE
                     VAPRRAGDPAILIASSDKAIEELNWKPKFNSLETIIETAWNWHKNHPNGYEK"
     gene            4962..5306
                     /locus_tag="K8M77_001859"
     CDS             4962..5306
                     /locus_tag="K8M77_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBI7086345.1"
                     /translation="MNKIVLLGRLVKDPELRYVEGGEKVYTKFTIAVARNFKSSTGER
                     KKDFIPIIMWEKKAEIACKYLKKGDLLSVSGRLITRSFEDTEGKRRYIVEVSVEDFKC
                     VNFNHDRVDNNF"
     gene            complement(5381..6580)
                     /locus_tag="K8M77_001860"
     CDS             complement(5381..6580)
                     /locus_tag="K8M77_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBI7086346.1"
                     /translation="MKHLNIKDDLFWVGALDPNLRVFDIIMYTPYGTTYNSYVVKGSE
                     KTAIFETVKAEFFDQYIARLKDLGVEPSEIDYIVVSHTEPDHAGSIGKILELAPKAKI
                     VASPIAINYIKEIVNGDFESIPVKDGDSISLGNKTLKFMSVPFLHWPDTIYTYVEEDK
                     TLITCDSFGSHYSCEEMFDDLIPNEEEYMEALKYYFDCIMGPFKPYVLKALDKIKGLD
                     IEIICTGHGPILRSNPYKVVNLYKEWSTPAPAKEVKKASIFYVSAYGYTKQLAEKIAE
                     GIKSTGNLDINLFNIIEHDMTELVGEVGSSDAILFGSPTIVNELVEPVRDLMAKLNPV
                     IHGGKIAGAFGSYGWSGEAVPRIESRLKELRMNMPVEGLRIKFKPSEEEAQKAFDFGV
                     EIGNALK"
     gene            complement(7059..7948)
                     /locus_tag="K8M77_001861"
                     /pseudo
     CDS             complement(7059..7948)
                     /locus_tag="K8M77_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480024.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(8079..>8320)
                     /locus_tag="K8M77_001862"
     CDS             complement(8079..>8320)
                     /locus_tag="K8M77_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7086347.1"
                     /translation="SQKFFEFKTKLTVKCKENNIELRIVDRFYPSSKTCSQCGEIKKD
                     LKLSDRVYKCSCGFVIDRDLNASINLRNTKEYKIA"
BASE COUNT         2834 a         1185 c         1146 g         3155 t
ORIGIN      
        1 aaaataaaat agaaggtatt aacttgtaat gtgattataa atactatata atacataata
       61 agggtttaaa acgaaatttg aggtgtgcta tggatattaa taattttaat tctgaacaaa
      121 taaaattttt ggcagaagaa atgtcaaaaa acagtatggt ttcttatgat gatttaccaa
      181 agtatgattt atttctttca caggttatag actatctaaa tgataaattt acggaagata
      241 aatttactaa taatatagta caaaattata ttaaaagtga ggtaatatca aagccggagg
      301 atgggaaaaa gcgtggatat actaaagtac acttagctca attggtttta ttaagttata
      361 tgagaccttt attaaaaacc gatgagatca aaaaagtatt tgctttagcc tttaataata
      421 ttaatgatag aagtgatgat ataatatctt gggaaacggc ttataagatg tttggcgaag
      481 tacaagaaga gagcataaaa agattcttag aggtgccatt agttgacaac gataaaatag
      541 aagaaataat acaggtttct gatataaaag aagaagatag agattgcata gtgactttcc
      601 taactgttat gacattaata gcacaagcaa gtgtagtaaa gaaagtagtg caaaaaatag
      661 ttaatggata tcataaagaa gaaaaataaa aaagttgaag ataaagcttc aactttttta
      721 tttttttatt caaagaaaga tatattatag ttacaatgga attctttatt aggaataagt
      781 gactggattg cagctttatc cttaaattct ccattaaatc catcaaagtc tgcatgtcca
      841 aaccaaggtt ctatacaaac aaatggagca ccagtatctt ttgtccataa tcctaagtaa
      901 ggaaattcac caaaatcaaa ttctaggtac tttttatgat tttttgattt taatgaaatt
      961 ttatttgatt ttagattttt aaaaactaaa gcatcatttt taaataaatc agtagataga
     1021 tttattatat tttcatcctt taaatatgat ttttcttctt ttgagaaata accaggatca
     1081 actaattgca ttatgcttgc attttctttc ttattaaatt cgaagtagta atcatcaaat
     1141 ttatcatttt caataggaca cataaatgct ggatgagcac caattgagaa atacatttct
     1201 ttattatcta aattctttac aatataatct atgcttaaat ttatatcatt taaagtgtat
     1261 tttattataa gtgaaaattt aaaaggataa acttttaatg tatcttcaga gtattttagt
     1321 tcaaaagata ttgagttttt agtttgttct atcatttcaa agtcacgaac tctagctaat
     1381 ccatgttgag gaagttcata agtaatgcca tcaactgtgt atttaccatt aaatacttta
     1441 ccaactatag ggaaaagtac tggagcatga tatttccaat aagcttcgtc tccagatcat
     1501 aaaaattcag tattatcttt ttttcccttt atactatgta attcgccacc ataattactt
     1561 aaagagattt ttaagtgttg attttcaaga gtataaatca taatctcatc ctttcagagt
     1621 agtctatttt aaaataatta tatcattaat tctatatagt aaaaaagctg aaaaacaatc
     1681 ttttgagatt atttcccagc tagttagtat attattttgg attaacatcc ttatatatat
     1741 agttttgtat ttgttccttt gttttagctt catcaaatgt taaataccaa acaccatcaa
     1801 tcatttcccc tttgcaatat ccatttagag ggaaacgcat attttcaaca ggtgggatac
     1861 cagttgaagc aactgattta gcaatactaa gtattttaga tggagttaaa tttgtttcaa
     1921 cataaggtaa aaattttttt attgtttcag gatattcaga aggttttaat gttgaaagtt
     1981 tctttatgat tgcatcaaga acatttcttt gtctttcagt tctttcataa tctccatcac
     2041 caacataacg aattctagag taacctacag cttgggcacc atttaatact tgctcacctt
     2101 cactaactgg cgtatattct cttccagcaa tttctgcaac atttttagcg taattattaa
     2161 cttccttaac ttcataaggt tttatattta ttggtactcc acctaaatca tctattattt
     2221 cagctaagtt atcaaagtta acagttacat agtcttttat atctaatcca aagttttcat
     2281 ttattgtttt taatgctaaa gtaggaccac cataagcata agcatgagtt aatttagttt
     2341 taccatgtcc atcaacttca acataactat ctctcataag agatgtaagt tttattttat
     2401 catgtttagt atctattgta gctattatta ttgaatcaga gcgtccaaca ttattatgat
     2461 ctctgctatc tactccaaaa agagctatat ttaaaatacc atcttggaat aggtctttat
     2521 ttgattcatc aatacctaaa gatttatcat cttgagctat tttctttata tccattttat
     2581 ttaatgttga gtaaacatat gtagctccaa gtcctatacc tataagtaat aagaagaata
     2641 atgttaatac tattattttc actttacttt ttttcttagg tgtattacct tgactattag
     2701 aatcattctt ttttgcatgt ttgccttgcg acattaattt caccaacctt cgccctttaa
     2761 aattttacac aatttactag tataatttta ttcctataga ttaacaaaaa agttacattt
     2821 ttattaatta tacatattaa ttataaatgt gtataattaa tatatctata gttaattata
     2881 aaattagtat tatttataag gagtaattta tgaaaaagaa atcaaaaggt tttctgagaa
     2941 aaactgtaat atttcttgtt atgtttataa aaaaaatagg agatgacgat atttttgcat
     3001 taggagctca attggcatat tatatggtat tatcttttat tcccttttta atgttcttaa
     3061 tgaatttagt aggttttagc catttaaata gtgatactat tcttaatttg ttgagtaatg
     3121 taatgccaac agaagctttt aacctaattc aaagtactgt tattgaaata gtagacaaag
     3181 aacaaacagg attattatgg ataagtatag ctttagccat atgggtatca tcttcaggat
     3241 ttaaagctgt aataaagggg ctgaataagg cctatggagt taaagagact agatcttata
     3301 taaagttaaa attaatttct atgatataca caattttatt agctttaatt gttatagcta
     3361 ctttattttt atttgtattt ggtgatgtaa taggagattt ctttataaaa gtattagagc
     3421 atccggaagt tatatattat atatggaata tgttaagata tgtagtagtt attttaataa
     3481 tggtattatt ttttatgttt ttatataatg caactccttg tgtaaggcta ggatggcttg
     3541 aggtgattcc aggagctgtc ataacaacat tagggtggat atcaatttca tatatttttg
     3601 cctattatgt aaataatttt agtaattact caagattata tgggagtttg ggagcagtat
     3661 ttatgtttat gacatggatg tttataactt ctatgatatt aattttaggt ggagaaataa
     3721 atgctgtttt agctgaaaaa aatagattaa aagatttata gagaaacaaa aaagagtcat
     3781 ttaaccatta aggcaatgac tctttttata attataattt attatttttc atatccattt
     3841 ggatggtttt tatgccaatt ccaagcagtt tcaattattg tttctaatga attgaattta
     3901 ggtttccaat ttaattcttc tatagcttta tctgatgaag ctattaatat agcagggtct
     3961 ccagctcttc taggagctac ctcagctggg atttggtggc cagttacttt tcttgcaact
     4021 tctacaactt cttttacagt aaatccagta ccattaccaa gattgtatat tctgctttct
     4081 ccaccattca ttaatctttc tagtgctaat gagtgagcag atgctaaatc tgatacatgg
     4141 atataatctc ttacacaagt accatcttta gtatcataat catctccaaa catcattatt
     4201 ttatctcttt ttcctagtgc aacttgtaat attaaaggaa ttaaatgagt ttcaggagca
     4261 tgatcttctc caatttttcc gttaacatga gcgccagcgg cattgaagta tcttaaagca
     4321 gtgtatttta ttccataagc agtatcacac cattttaaaa tcttttcaac aagaagctta
     4381 gattcaccat aagcatttgt aggaagattt ttatcagttt ctaatatagg aatattttct
     4441 ggttctccat aagtagctgc tgttgaagag aaaactatgt attttacatt atgatctctc
     4501 atagcctcta ataagcttat agttcctgaa acgttattat tgaagtattt taaaggttct
     4561 gtcatacttt ctccaacaag agagtaggca gcaaagtcaa taacagcttc tatattattt
     4621 tcagtgaaaa tcttgtctaa tatttttcta tctcttaaat cacctttata aagctttcca
     4681 ccaagaatag catcttcatg tcctttttcg aagttatcta agataacaac ttctttatta
     4741 ttttcaatta attctgcaac catatggcta cctatatagc cagcaccacc acaaactaaa
     4801 attgccatgt taatacctcc agtaaatttt attctaataa cattatatac tattttaagt
     4861 aaaagtttta ctaaaataaa cgaaatttaa tatgaatttt gaataaattt cacttttttg
     4921 gttaaaataa caggtgagaa ttttttagga ggtcatatta aatgaataaa atagttttat
     4981 taggacgttt agtaaaggat ccagagttaa gatatgtaga ggggggggag aaagtatata
     5041 caaaattcac aattgctgtg gcaagaaatt ttaaatcttc cacaggagaa agaaaaaaag
     5101 attttattcc aataataatg tgggagaaaa aagcagagat agcttgtaaa tatctaaaaa
     5161 agggagatct tttaagtgtt agtggaagac ttataacaag aagctttgag gatactgaag
     5221 ggaagagaag atatattgta gaagtatcag tagaagattt taaatgtgtt aattttaatc
     5281 atgatagagt agataataac ttttaatata aaaaagtagt aagcaaatag ttacttacta
     5341 ctttttggtt aatgtatatt tagtataatt aaattaagaa ttattttaaa gcatttccta
     5401 tttcaacacc aaagtcgaaa gctttttgag cttcttcttc tgatggctta aatttaattc
     5461 ttaaaccttc aacaggcata ttcattctta attcttttaa tctactttct attctaggaa
     5521 cagcttcacc actccatcca tatgaaccaa aggcaccagc tatttttcca ccatgaatta
     5581 cagggttaag ttttgccatt aaatctctta ctggctcaac aagttcattt acgattgttg
     5641 gagaaccaaa taatatagca tctgatgaac caacttcacc aactaattct gtcatatcat
     5701 gttctattat attaaataga tttatgtcta agtttccagt tgatttaata ccttcagcta
     5761 ttttttcagc taattgttta gtatatccat atgcagaaac atagaatatt gaagcttttt
     5821 taacttcctt agctggagct ggagtactcc attctttata aagattaacc actttatatg
     5881 ggttagatct taagattgga ccatgacctg tacagattat ttctatgtct aatcctttta
     5941 ttttatcaag agcttttaaa acatatggtt taaatggtcc cattatacaa tcaaagtagt
     6001 attttaatgc ttccatatat tcttcttcat taggaattaa atcatcaaac atttcttcac
     6061 aagaatagtg gcttccgaat gaatcacaag tgataagagt tttatcttct tctacgtaag
     6121 tgtagattgt atcaggccag tgtaagaatg gtactgacat gaattttaaa gttttatttc
     6181 ctaaagaaat tgaatctcca tctttaactg gaattgattc aaaatctcca ttaactattt
     6241 cttttatata gttaatagct ataggtgatg ctactatttt agccttagga gctaattcta
     6301 atattttacc tatagaacca gcatggtcag gctcagtatg acttacaacg atatagtcaa
     6361 tttctgaagg ttctacacct aagtctttta atcttgctat atactgatcg aagaattcag
     6421 cctttacagt ttcaaatatt gctgtttttt cactaccttt tacaacatat gaattataag
     6481 ttgttccgta aggtgtatac attattatgt caaatactct aaggtttgga tctaatgctc
     6541 ctacccaaaa taaatcgtct ttaatattta agtgcttcac tacatacgct acatattttt
     6601 tgtagaataa tatgcagcta cctcccttca aaatgtaata ttttttataa attaagaatt
     6661 attttataca taaacctaaa atgataataa attaattaaa agtacaaatt agctttatat
     6721 agaattatta atgattattt cactatatta aacaaattta taaatatttg ataaaattat
     6781 ataaaaagtg taattattct tttaattaat taataatatc aattaataaa atttattatt
     6841 tgatattatt aattatacac ttttagggtt aaaagtaaat agaaattggg cattattaac
     6901 tgatttttaa actaaaaaac aattgagaat tttcagaaaa ttagtatgaa tatttaataa
     6961 gttttagtta aaaactgcat gaaataatgt aataaattgt aaaataacca cactttagta
     7021 tggctatttt aaattcattt ttatttataa ttaattattt attttctttt tttctttaaa
     7081 gttaaataaa caccagtaga tgataaagca attccagtag tagctaaagc accagcccct
     7141 ataggtaatc ctgtattagg taaatttcca ttatcttttt tatttgtagt aatttctaat
     7201 gatattttat ctgattcttt tccactttta tcaattgaag atacttggaa gttatattta
     7261 gtattaggtt ttaagtattt agctaagaac tcaagtggag ttttgtcagc aggaactcta
     7321 ccaatttctt ttccatcttg ataaataaca tactctttaa gtctactttt tcatgagctt
     7381 tccaacttaa taaagcacta ttttcagtaa tttctttact aactaaagca gatggctcag
     7441 aaggtttttc acctgaattt tctgtatcac caggtttgtt tggattttct ggtttttcag
     7501 aatcaccagg ttgatcagga ttaaaaggtt tatctgtatt accaggatta tctgggtttt
     7561 caggattgtt tggtttgtct ggatttactg gcttatcagg atcaactggt ttatttgaat
     7621 catcagggtt tattacaggt ggtaaatcca catttccatc taatcccttt acagaaactt
     7681 cgtatattct agctgcagag tctgcacctt gagttggctg aataatattt aatctaacat
     7741 atctagcttt agtaacaggg aaagcatcat tgcttatagc ctttatatta ttatctacat
     7801 ttaatacagg agtaaatttt tcaccatcat tactaacttc aagagagtaa gctttagtgt
     7861 tcataccact gctttctcca cctgcttctg catggcttat ttctaattct cctatagttt
     7921 taatatctgc tactcaaaat atggtcaaat atatccatat agagattatt ccttcttcat
     7981 agaacctcgc cttgcctaag ctactacgat tttttatggc tctccaaagg cttaaattcc
     8041 gaagttagcc ctccgtacat atataagtgc ttttcatttt aagctatctt atattcttta
     8101 gtatttctaa gattaatact tgcgtttaaa tccctatcta ttacaaatcc acaagaacac
     8161 ttataaactc tatctgacag ttttaaatcc ttcttaatct caccgcattg actacaagtc
     8221 tttgatgatg gataaaatct atcaactatt ctaagttcta tattattttc tttacattta
     8281 actgttaact tagttttaaa ttcaaagaac ttctgacttg
//
LOCUS       DAEVGI010000105         8283 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEVGI010000105 DAEVGI010000000
VERSION     DAEVGI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.105"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     regulatory      46..150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            242..937
                     /gene="thiT"
                     /locus_tag="K8M77_001863"
     CDS             242..937
                     /gene="thiT"
                     /locus_tag="K8M77_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBI7086348.1"
                     /translation="MMSFSQIINDFIQSFSSINLGGNFKEIVTNPLTILTLIGVLIIL
                     VLLIKAKDIKFDAKMLTTIGIALALTAVLNMLKIYKFPQGGGITLGSIIPIILISYAY
                     GPLVGMLTGFLFGIISLFIDPYILHPVQVLFDYPIPYMALGVAGFFKNHRISGAIIAI
                     FLKFLAHFISGVVFFGAFAPKGMSPILYSFLVNGSLIGADGLICVVLLCILPVNRLIK
                     AISSKSVTSKYTS"
     gene            1046..2122
                     /locus_tag="K8M77_001864"
     CDS             1046..2122
                     /locus_tag="K8M77_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI7086349.1"
                     /translation="MSINHGANLFDLSNELGINKKDIKDFSSNINPFGASKKAKDAIL
                     NNIDMVSIYPDPKYKDLKESISQYCHCKKENIIVGSGATELISSFISVINPKKALLLS
                     PSYSEYESELEKINCEITKFFSKEEDNFKIDVNKLIDSINSSRFDLVIICNPNNPTGF
                     AFSKDEISLLLKNTSSIFMVDETYVEFTEPEVYSSTPLVDIFDNLFVIRGTSKFFSTP
                     GIRLGYGLISNKEIKKSMVEKLDLWNINIFATTMGEIMFKDKEYILSNTSKLKEERDY
                     LFRELSSIKDLKVYESYSNFILCKITSKKFTATKLYNKLLEKGLIIRNCSSFEGLNEY
                     FFRVCVLKPEDNKLLIENLKNLFL"
     gene            complement(2263..3309)
                     /gene="cax"
                     /locus_tag="K8M77_001865"
     CDS             complement(2263..3309)
                     /gene="cax"
                     /locus_tag="K8M77_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HBI7086350.1"
                     /translation="MKILKWLLIFIPISIIGEFMHFSPTLMFILSALAIVPLAGLMGE
                     ATEEISFYTGPKLGGFLNATFGNATELIISFFALKDGLFDVVKASIAGSVIGNILLVL
                     GASMLAGGLKYKTQSFNKKVMETSSSMLLFSVIGLTIPAIFMHTIKPEFLNTKYESLS
                     VIVAIVMFAIYILSLIFSFFTHKDIYAVEQDEEDGEAKWSLKKAITVLIVATVIIAIE
                     SEFLVGAVEPMTEALGLSKLFVGIIIIPIIGNAAEHSTAIIMAMKNKMDVSVEIAIGS
                     SLQVILFVAPVLIFLSLIFKPMSIIFNAFELVALIVSVLIANRIVNDGESNWLEGVQL
                     LAVYIIIAAMFMIV"
     gene            complement(3747..5030)
                     /locus_tag="K8M77_001866"
     CDS             complement(3747..5030)
                     /locus_tag="K8M77_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBI7086351.1"
                     /translation="MNNKYMVVISLDAVSSKDIEIMKELPNISKLMKEGALIKNVETI
                     YPSLTYPAHVSIITGKYPVNHGITNNIVLNFKDENPNWNWYGNKIKGETLFGVAKKNG
                     LTTASVVWPVTGKMKIDYNMPEIFETKPWHNQIIMSALAGSKVYQFSMNKKFGSLRKG
                     TKEPYLDNFVTACAKETIKKYKPNLTMVHLIDVDSHRHDYGYENLEALEALKRHDIRV
                     GEIIECLKESGIYENTTLILLGDHSAIDTHKVIRLNSIFREEGLIELKDGKLKSFKAI
                     ARECGGSCYIYLDNKNKKYENKCLEVLERLKEEGALEYILNKEEAIKAGANDQCDFMV
                     EATRGYYFSNEYEGKVIEETKEIKNKHTYKAVHGYSPRKKDYGTFFLAFGRGIKSGVV
                     LEEGKLINHGPTLGRILGFNFEKADGIVEERILNL"
     gene            complement(5504..5842)
                     /locus_tag="K8M77_001867"
     CDS             complement(5504..5842)
                     /locus_tag="K8M77_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086352.1"
                     /translation="MGSKVFERKNRVYDLKYSDDISKICKPYKIASNIAWIVVVIALV
                     EPRVLLPTFLLSLLMFLSKKMKVSWRVSTFLEKVISGRSDEGEKILSKLQGDIEDPIY
                     KELKELLKIK"
     gene            complement(5981..6394)
                     /locus_tag="K8M77_001868"
     CDS             complement(5981..6394)
                     /locus_tag="K8M77_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086353.1"
                     /translation="MFLKELNKEQGLAFINLVTEFALADENIKKEEEDLIRTYMKELD
                     LEEEELGNLSYEESIETIKNSSEKVKNIVYFELVRIGLVDEDCDIEEVDYLEKISKDL
                     NISRAKKIQVANCFYNFSEKDGEEKLEEMAKDIIG"
     gene            complement(6595..7506)
                     /locus_tag="K8M77_001869"
     CDS             complement(6595..7506)
                     /locus_tag="K8M77_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBI7086354.1"
                     /translation="MYVINFIRGFLMALADSVPGVSGGTIAFIMGFYNKFISSLNALT
                     STKKTDVNKKDAIIFLLKLGIGWITGMVLSILFIASIFDKEIYKISSLFTGFIIFSIP
                     IIISEDKESLKKNYGYIIYAILGIAIVAAITYFNPATGSAKGTSLVLNEFSLPLALYV
                     FFAGMIAISAMVLPGISGSTLLLIFGLYAPVVNGVKEVLTFNFSYLPMLMVFGLGVVV
                     GIITTIRLIKFLLSNYRSQMIYFIIGLMIGSIYAVFMGPTTLKIPKAAMNLSSFSFLF
                     FIIGGALILGLQQLKGYLEKKEKVSNI"
     gene            complement(7540..>8283)
                     /locus_tag="K8M77_001870"
     CDS             complement(7540..>8283)
                     /locus_tag="K8M77_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBI7086355.1"
                     /translation="ITSFFIGRSYESYKFKALEVSNVSSNEEIDNENKESNKEELSEE
                     QLRIKKIILNDKDGLFKVVNKENSIDKSYKPEELIIPKVNFVGLENEPRNRISNVLKK
                     DLENLFSDAKKAGLDLYLSCAYRSYEEQEYLYGRALEKEKSDSSNLVALPGTSEHQLG
                     LAVDLTTRSIGFQLEESFEDTKEGQWLLENSHKYGFILRYLKGKEDITGYSYEPWHYR
                     YVGDLEVSKYCYDNNLTLEELYDELDITK"
BASE COUNT         2878 a         1331 c          843 g         3231 t
ORIGIN      
        1 ccttgaccta attataattt gcatataaaa tatacttaga attatttcat gtgctagggg
       61 tgcctttagg ctgagagatg attattttat cattaaccct caacacctga tctggttaat
      121 tccagcgtag ggaagcgttt agggctgtat ttaaactgga tctatattta gattcggttt
      181 ttttatttac attctttaga ctctctaagc acacttaaag tttaaggagg gggtaaaaac
      241 tatgatgtca ttttcacaaa taataaatga ttttattcaa agtttttcat caattaattt
      301 aggaggaaat tttaaggaga ttgtaactaa tccactaaca attctcactt taataggagt
      361 cctaataata ttagttttac ttataaaggc taaagacatc aaatttgatg caaaaatgct
      421 tacaacaata ggaattgctt tagcacttac tgctgtatta aacatgctaa agatatacaa
      481 atttcctcaa ggtggaggaa taactttagg tagtataatt cctataatac ttatttctta
      541 tgcttatggt ccattagttg gaatgcttac tggattttta ttcggtataa tttccttatt
      601 tatagatcca tacatacttc atcctgtaca agtattattt gattatccta taccatatat
      661 ggcattagga gtagcaggat tctttaaaaa tcatagaatt tcaggagcta taatagcaat
      721 atttttaaaa tttttggctc actttatttc tggagttgtt ttctttggag cctttgctcc
      781 taaaggaatg tctccaattc tatattcatt tttagtaaat ggttctttaa taggagctga
      841 tggtttaata tgtgtagtac ttttatgtat tttacctgtt aatagactta ttaaagccat
      901 aagttctaaa agcgtaacaa gcaaatacac atcttaaaat aaagtaaggt ctagatttca
      961 ttaaaatcta gaccttttta ttttttaatt aaagaattat actataggta agagataaaa
     1021 agttatataa acttaggagg tacttatgag tattaatcat ggtgctaatt tatttgattt
     1081 atcaaatgaa ttaggtataa ataaaaaaga tattaaagat tttagttcaa atataaatcc
     1141 ttttggagca tctaaaaaag ctaaagatgc aattttaaat aacattgata tggtttctat
     1201 atacccagat cctaaatata aagatttaaa ggagtcaatc tctcaatatt gtcattgcaa
     1261 aaaagaaaat attatagttg gtagcggagc tacggaacta atatcctctt ttataagtgt
     1321 tattaaccct aaaaaagctt tattactttc tccatcttat tcagaatatg aaagtgaact
     1381 tgaaaaaata aattgtgaaa taactaagtt tttttctaaa gaagaagata actttaaaat
     1441 agatgttaat aaattaatag atagcataaa ctcttcaaga tttgatttag ttataatttg
     1501 taatcctaat aatcctactg gattcgcctt ttcaaaggat gaaatttctt tattactaaa
     1561 aaatacttca tcaatattca tggttgatga aacttatgtt gagtttacag agcctgaagt
     1621 ttattcctct actccactag tagatatatt tgataattta tttgtaatta gaggaacatc
     1681 taagtttttc tcaactccag gtataagatt aggttatgga cttatttcta ataaagaaat
     1741 taaaaagtca atggttgaaa aacttgattt gtggaacata aatatctttg ctacaacgat
     1801 gggagaaatt atgtttaagg ataaagagta tattctatca aatacctcta agttaaaaga
     1861 agaaagagat tatctattta gagaattaag ttcaataaag gacttaaagg tatatgaaag
     1921 ttacagtaac tttatacttt gtaaaattac atctaaaaag ttcactgcta ccaaacttta
     1981 taataaactt ttagaaaaag gattaataat aagaaactgt tcttcttttg aaggtttaaa
     2041 tgaatatttc tttagggttt gtgtcctaaa gcctgaagat aataaacttc ttatagaaaa
     2101 tcttaaaaac ttatttttat aattaaaaaa attaagaggt tgcatataca taatttttat
     2161 tattaattat atatgcaacc tcttaatttt tgaaattata ctttatttaa tatgataagt
     2221 actttttaaa attcacctat tttatttaat taatttttta atttaaacaa tcataaacat
     2281 agctgctatt attatgtaaa cagctaaaag ttgaactcct tcaagccaat ttgattctcc
     2341 atcgttaaca attctattag ctataagtac tgaaactatt aaagctacta attcaaaggc
     2401 attaaatatt atactcatag gcttaaatat aagacttaaa aatattaata ctggagccac
     2461 aaataaaata acttgcagac ttgatcctat agctatttct actgaaacat ccattttatt
     2521 cttcattgcc attattattg ctgtgctgtg ttctgcagca ttacctatta taggaattat
     2581 tattatcccg acaaaaagct tactaagtcc aagagcttct gtcatcggtt ctactgcccc
     2641 aactaaaaat tcactctcta tagcaattat tactgtagct acaataagca ctgttatggc
     2701 cttttttaat gaccatttag cttctccatc ttcctcatct tgttctactg catatatatc
     2761 cttatgagtg aaaaatgaga atattaaact taatatgtat atagcaaaca ttactatagc
     2821 aactattaca cttaaacttt catattttgt gtttaaaaat tctggcttta ttgtatgcat
     2881 aaatatagca ggaattgtta atccaataac agaaaataat agcatacttg aactggtttc
     2941 cattactttt ttattgaaac tttgtgtttt atattttaat cctcctgcta acatgctagc
     3001 acctagaact aatagtatat ttcctattac agaacctgca atagaagctt taaccacatc
     3061 gaacaaacca tcttttaatg caaaaaatga aattataagt tctgtagcat tcccaaaagt
     3121 agcatttaaa aaaccaccta attttggccc agtataaaat gatatttcct ctgttgcctc
     3181 tcccataaga cctgctaaag gaactattgc caaagcagat aatataaaca taagagttgg
     3241 agaaaaatgc ataaattcac ctataatact tataggaata aatattaaaa gccattttag
     3301 tattttcatc tttagtcctc catatataaa ataatttact aaggataatt atatcacttt
     3361 tgttaattat tttaacaaat tattattaat ttacctattt aaaatagtaa cattaaccta
     3421 cactttaact aatttttatt ttatcaatat attaaaacta aatatatctt agttatatct
     3481 ataaaaagat aattattatc tttttataaa acttaaatta tatatttagt atgcttataa
     3541 tcaacttcta ttatagagct tttttctaaa attattctta aaagcaaaaa aaacgctatt
     3601 ttaagcgttc ttttaattaa ttacatatct acattatgaa ataatttttg gcaatatttt
     3661 ttctaccatt ttaaaatatc ataattttgc gacaatttca ataattttat tcaattaatt
     3721 attttattca aaattataga aaattattac agattcaata ttctttcttc aactattcca
     3781 tctgcttttt caaaattaaa acctaatatt cttcctaagg ttggtccatg atttataagc
     3841 ttaccttcct ctaaaacaac acctgatttt attcctcttc caaaagctaa aaagaatgtt
     3901 ccataatctt tttttctagg tgaatatcca tgaactgctt tgtaagtatg tttatttttt
     3961 atttctttag tctcctcaat aacttttcct tcatattcat ttgaaaaata atatcctcta
     4021 gtagcttcta ccataaaatc acattgatca ttagctccag cttttatggc ctcttcctta
     4081 tttaatatgt attctaaagc tccttcttcc ttaagccttt ctaaaacttc taaacattta
     4141 ttctcatatt ttttattttt attatctaaa taaatataac atgagcctcc acattcccta
     4201 gctatcgctt taaagctttt aagctttcca tcctttaatt ctattaatcc ttcttcccta
     4261 aagatactat taagccttat taccttatga gtatctattg cactatggtc tcctagtaaa
     4321 atcaatgtcg tgttttcata tattcctgac tcttttaaac actctattat ttctcctact
     4381 ctaatatcgt gtctttttaa agcttctaag gcttctaaat tttcatatcc ataatcatgt
     4441 ctatgagaat ctacatctat tagatgaacc atagttaaat taggcttgta tttttttatt
     4501 gtctccttag cacatgctgt aacaaaatta tctaaataag gctcctttgt accttttcta
     4561 agagacccaa actttttatt catactaaat tgataaactt tacttccagc taaagcagac
     4621 atgattattt gattatgcca aggttttgtc tcaaatattt ctggcatatt ataatctatt
     4681 ttcatttttc ctgtaactgg ccaaaccacc gaagctgttg taagaccatt tttcttagct
     4741 actccaaaaa gagtttctcc ctttatttta tttccgtacc agttccaatt tggattttca
     4801 tccttaaaat ttaatactat attatttgtt attccatgat taactggata tttcccagta
     4861 ataatggata catgagctgg atatgtcaaa gaaggatata ttgtttctac attctttata
     4921 agagctcctt ctttcattaa cttagatata ttaggtaatt ccttcattat ttcaatatct
     4981 tttgaagata ctgcatctaa agaaattacc accatgtatt tattattcat tttaattatc
     5041 atctcctgtt cctattaaat tattttaatt cttttatcct ctatgtcaat gataatttta
     5101 tattactcaa tctttatttt atattttaaa tatataagtt tatcaaatat acatttattc
     5161 attcttacaa aataaagttt tattttatat actattaaca tatacattat aagtaaagcc
     5221 tatagtgagc atcaaaaaga aactactata gatgaaaatt ttcttttctc tgttgacgca
     5281 taacccattt agtattattt gtctattaat aactcctata agtgacaaaa ataaaacgaa
     5341 tataaaaatt ctatctttaa ctaaggaaat ataaataatt aaagataata tatttataaa
     5401 aatagctgaa ataaccggat ttttatataa aacattcttc atatcataac ctcctcccca
     5461 tatacaagaa ctaatctata ttttaactat aaattataat cttttatttt atttttaata
     5521 actctttaag ttctttataa ataggatcct ctatatcccc ttgtaattta cttaaaatct
     5581 tttctccctc atcacttctt ccacttatca ccttttctaa aaaagtactt accctccatg
     5641 atactttcat tttcttactt aaaaacatta ataagcttaa taaaaaagta ggcaataaaa
     5701 ctctaggctc tactaatgca attacaacta caatccaagc tatatttgaa gctattttat
     5761 aaggtttaca aattttactt atatcatcac tatattttaa atcataaact ctattttttc
     5821 tctcaaaaac tttacttccc atatattctc ctcctattaa taacttaaca attcaattat
     5881 aatcccatta tacatatttt tccatataat taaataaaaa aaggcttata ccaaaataat
     5941 ttttattctt atattttgat atagccaact ctatttttta ttatcctatt atatcttttg
     6001 ccatttcctc taatttttct tctccatctt tttctgagaa gttatagaaa caattagcta
     6061 cttgtatttt tttagctctt gatatattta aatctttgct tattttttct aagtaatcaa
     6121 cttcttctat atcacaatcc tcatcaacaa gtcctattct gacaagttca aaataaacaa
     6181 tatttttaac cttttctgat gaatttttta tagtttctat tgattcttca tatgaaagat
     6241 ttcctaattc ctcttcttct aaatctaatt ccttcatata tgtacgaatt aaatcttctt
     6301 cttctttttt gatattctca tcagctaaag caaattcagt tactaaattt ataaatgcta
     6361 atccttgttc tttatttaac tcttttaaaa acattgctta tccctccgta taaataaatt
     6421 tttagcttat tattaataat actataaaaa atttagtata acaaaggctt taaggttaat
     6481 tttcaattaa gaaatgacta caatcctcta tttctcttat tatattctaa aaatctacaa
     6541 tattaaagaa aaagctgtca ttacttctac gtaattacag ctactatata ttttttatat
     6601 attagaaact ttttctttct tctcaaggta tccttttaac tgttgcaacc ctaaaattaa
     6661 agctcctcct ataataaaga ataggaaact aaagctactt aaattcatag cagcctttgg
     6721 aatttttaat gtagttggtc ccataaaaac tgcatatata gatccaatca ttaatcctat
     6781 tataaaataa atcatttgag acctataatt tgaaagtaag aattttatta atcttattgt
     6841 agttattatt cctacaacta ctcctagtcc aaaaaccata agcataggta aataactaaa
     6901 attaaatgtt aaaacttctt tcactccatt tacaacaggt gcataaagac caaatattaa
     6961 aagtaatgtt gatcctgata ttcctggtaa aaccattgct gatatagcaa tcattccagc
     7021 aaaaaaaaca tatagtgcaa gtggtaatga aaactcattt aatactaaac ttgttccttt
     7081 tgcacttcct gtagctgggt taaaatatgt tattgcagca acaatagcta ttcctaaaat
     7141 agcatatatt atgtatccat agtttttctt taatgattct ttgtcttcac taattattat
     7201 tggtatagaa aatattataa atccagtaaa taaagaactg attttatata tttctttatc
     7261 aaatattgat gctataaata atattgataa aaccatacca gttatccaac ctattcctaa
     7321 ttttaataag aatattatag catctttttt gtttacatca gttttttttg tagatgttaa
     7381 agcatttaat gaacttataa acttattgta aaatcccatt ataaatgcta tagtaccacc
     7441 agaaacccct ggaacactat ctgctaaagc cattaaaaac cctcttataa agttaatcac
     7501 atacatagac tatctccttt aaaatcttat atttatatat tattttgtta tatccaattc
     7561 atcatataat tcctctaatg taagattatt atcataacaa tatttgctta cttctaaatc
     7621 tccaacatat ctatagtgcc aaggttcata actataccca gttatatctt cctttccttt
     7681 taaatatctt aaaatgaaac catatttatg agagttttct aaaagccact gtccttcttt
     7741 tgtatcctca aaagactctt ctaattgaaa ccctatagat ctagtagtaa ggtctacagc
     7801 taatcctaat tgatgctcac ttgttcctgg taaagccaca agattgcttg aatcactctt
     7861 ctctttttct aaagctctgc catataaata ttcctgctct tcataagacc tataagcaca
     7921 agaaaggtat aagtctaagc cagctttttt tgcatcacta aaaagatttt ctaaatcttt
     7981 ctttaataca ttgctaattc tgtttctagg ttcattttct aaacctacaa aattaacctt
     8041 tggaataatt aattcttctg gcttataact tttatctatt gagttttcct tatttacaac
     8101 cttaaacaat ccatccttat catttaaaat tattttttta atacgaagct gctcttcact
     8161 aagctcttct ttatttgatt ctttattctc attatctatc tcttcattag aacttacatt
     8221 actcacctct aaagctttaa atttgtaaga ctcatagctt cttcctataa aaaaactagt
     8281 tat
//
LOCUS       DAEVGI010000106         8227 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEVGI010000106 DAEVGI010000000
VERSION     DAEVGI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.106"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            232..2397
                     /locus_tag="K8M77_001871"
     CDS             232..2397
                     /locus_tag="K8M77_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBI7086356.1"
                     /translation="MKWSNILKVFKRDMKAIIKNPIALLIIGGICFIPSLYAWVNIAA
                     CWDPYENTSSVPIAVVNNDKGSSFNGKEMNIGNEVIDELKNNHAIGWKFVDTKQANRG
                     LIDGTYYAMIEIPEDFSKDLTSVLSENPKKPEIIYKVNTKANPVAGKITGVAQSTLVN
                     QITSNFITTVNETAFSSLNDLGYDVNKNKDNIIKLKNSIIAIDKNMSSIKNILNNINS
                     NSANLSTYLKEVQNTMPSITNGLNSISKNTDNTGNLINNSKESLNSTFDNIRLNLSES
                     QTSLNKIQSNLDELSSIASDASSAKINSLISKSINEINKVDNSITVVTNFLDVINKKN
                     NNEKIANMITSLKNIQNSLAEEKTKLNDLQSKVNNGKDLDKGLLSSISDLTKKIEGQL
                     DNSLNRFDNDTRPALNTIADGFVTATKGASDLLGKANGLVDQINNLLSTANQGAQLAN
                     STSEKLKNSLEEFSGVISELSSKLKDVSDDDLGKIVGILQSNPEFMGDFIANPFNIEV
                     DAIYKVANYGSGMAPIYSVLALWVGSLILISLLKTESAEFEGSENINLRERHFGKMLT
                     FVSLGILQGFIVAFGDKFLLGVQTVNTALLIFVSMFASVVFTIIVFTLMSVFGNLGKA
                     LAIILMVLQLAGSGGSYPIQVDPLFFRIIQPTFPFTYAISGYREAIAGPLVSTVILDF
                     VVLTIMGLVFILLGYLLKGPLNPRVRKFEDMFEESGIAE"
     gene            2420..4576
                     /locus_tag="K8M77_001872"
     CDS             2420..4576
                     /locus_tag="K8M77_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBI7086357.1"
                     /translation="MKKIFKVFRRDLRNIRKNPAALSVVIGLCIIPSLYAWINIKACW
                     DPYVNTGNLPVAVVNNDKGAEINGKEINVGKEVINELKKNDAIGWQFVDSWQGNYGLN
                     EGKYYAMIEIPSDFSEGLTSLTTGNPQKPNIIYKVNEKSNAIANKITNVAKNKVTDEI
                     KSNFVDTVNKEAFDLLNQLGGKLETNKPEILNVRDTLQSANSSLKEIQDFVQKANGDS
                     KSLSSYLSSVRNDLPKVTDQISNLQTTVEASKSLINSTQQTVNAVSNSFSSDVVTMQS
                     TNQELQNLLSKLKELNNAMDTTKMVETLDKLLKLTDSLNKIIDNNIKSLEKINSLTPN
                     DKIVDLINNLNNAKDLVNKEKDQLVDLKTALSNNVSKEDIAKAIDVTSKISNELSSTL
                     INISNNFYNNGAPALNTIGNSLKGGLDTANSVLETTKIVVPQLNALTTFGIASSDVAS
                     QQADQITSKLNEFQDEISKLIEKTSGLTEENLNNIIDVMSKNPEEIASFISSPIKVKE
                     EQVYDAGIFGVGLTPFYTTLAIWVGSLLLTSLLTAECEDFEDGTKLNLIQKHFGKMLL
                     FLTISLIQTVIVVLGDIYILGVNPANMPLMFGFAIVSSIVFTVMIFTLVSLFGNIGKA
                     IAIVIMVFQVAGAGGIYPIQTNPKIFEILQPLWPFTYSIDGFREAIAGPIWNSVSKDM
                     KALGIFLLIFLLLCILKKPFHRLTEFVEHKFRESGL"
     gene            4705..6189
                     /locus_tag="K8M77_001873"
     CDS             4705..6189
                     /locus_tag="K8M77_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBI7086358.1"
                     /translation="MGRKEKNLYKAVDIKNKEIIDVREIGYYSTPPFVARYIGKRIID
                     INGKGKTLFDPCCGKEELTDYFSDLGIKTIGMDLIKYKNNYKCEFKKDNFINYYCSQK
                     NTKTWDYDYYIANPPYNCHEVNFIKENKERLKNYFNEVGLHNMYSMFMFAIIDKAKNG
                     ALIGLITNDSFFTAKNHKRLRNKILRECSIYEITMCPRGLFHNQGADVRTSILILRKG
                     KEYQEKIVVNNRPKSIEEFKGLLNGVKNNSNERLYSLQEIVLQNKKDNLEFLIECPED
                     IRSLFNNIRIGEKFKCVTGISTGNDKEFLSKEKSNEFTIPFYKNPGKDKFYTDRVIYL
                     HKNFLEIEKINRNFNVRNKEILYKSGITCSSMGVSFNACILPKNSTFGVNPNIICESE
                     EVWWLLAYLNSSLVTYLVRGALIRSNMITSGYVSRIPLLSFTKEEKAKLSHLAKRSYK
                     LRKQNSSIETELCHIDNIVNHVAKISNESKDLISNFKKYLVEVT"
     gene            complement(6191..6664)
                     /gene="msrA"
                     /locus_tag="K8M77_001874"
     CDS             complement(6191..6664)
                     /gene="msrA"
                     /locus_tag="K8M77_001874"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBI7086359.1"
                     /translation="MKKIVLAGGCFWGVEEFLSRINGVVSTEVGYANGRTENPTYEDI
                     CTKNTYFAEVCLVNYDENIISLKELLAKFWTIIDPTSLNKQGNDVGSQYRTGIYYVEP
                     SDLEEILNSKEELQKSYSKKIVTEVKPLENYYKAEEYHQKYLKKNPNGYCHIKLD"
     gene            complement(6716..7303)
                     /locus_tag="K8M77_001875"
     CDS             complement(6716..7303)
                     /locus_tag="K8M77_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBI7086360.1"
                     /translation="MAMTEKEKMILGLPYNPADPELTKGRIRARILFQKFNKLDLPDN
                     EEEADKTFKERSSILKELLGSTGESIYMEPNFKCDYGYNISVGNNFYANFDCVMLDVC
                     KITIGENCFFAPGVHIYTATHPIDPIERLKYEFGKPVTIGNNVWIGGHATINPGVTIG
                     NNVVVASGAVVTKDVPDNVVVGGNPAKIIKKVCDK"
     gene            7435..8223
                     /locus_tag="K8M77_001876"
     CDS             7435..8223
                     /locus_tag="K8M77_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3888 domain-containing protein"
                     /protein_id="HBI7086361.1"
                     /translation="MFDPKMLNVEFRNGITDEKPIEGRKYTLTHNDDTGELFLTVALA
                     YAYDKINSSARDEVFGVWKRGNNRFILKIYLYVDGNEGREEAINRDRIFRKELPLALA
                     AIAYGDREFLDDNDDLLNSNIIVRFISAYEELNKIENYGTLKNCTNNRMENTPNNNNN
                     IYIEESILNDNDPLVNNFILTLLNPYIKAEITKLYESDMYYCLKDVEVLEVFKAQADN
                     ECEFNYKIYVGVKVGTMPTSYNNLIIEFLVTPNAVSTLSVKNPR"
BASE COUNT         3222 a          936 c         1309 g         2760 t
ORIGIN      
        1 ttatattgat atataataag aacataatga tttaataaat taattaatat aatgtaattt
       61 gaaaacgata aattaagaaa aatttataaa atactatcaa aaaggtttat ttgtaaacta
      121 aattttgata ctatagtaat taagagtttt ttaagtattt taaatttata attttaagat
      181 aagacatagg gggatgtgtt tattttagct ttattaaatg gaggtgttta aatgaagtgg
      241 agtaacattt taaaagtgtt taagagagat atgaaagcaa taataaaaaa tccgatagca
      301 cttttaataa taggtggaat atgctttata ccatcgcttt acgcatgggt aaatatagca
      361 gcatgttggg atccttatga aaatactagc agtgttccaa ttgccgtagt aaataatgat
      421 aaaggttctt cattcaatgg aaaagaaatg aacataggta atgaagttat tgatgaatta
      481 aaaaataatc atgcaattgg atggaaattt gttgatacaa aacaagcaaa taggggatta
      541 atagatggta cctattatgc aatgatagag attccggaag atttttctaa agatttaact
      601 agtgttttat ctgaaaatcc taaaaagcct gaaataatat acaaggttaa cacaaaagct
      661 aaccctgttg caggtaaaat aacaggagtt gcacaaagta ctcttgtaaa tcaaataaca
      721 tcaaatttta taactaccgt taatgagacc gcattttctt cattaaatga cttgggatat
      781 gatgtaaata aaaataaaga taatattatt aagttaaaaa attctataat agctatagat
      841 aaaaatatga gttctattaa aaatatactt aacaatataa atagcaattc agcaaactta
      901 agtacttatt taaaagaagt tcaaaatact atgccatcaa taacaaatgg tttaaatagt
      961 atatctaaaa atactgataa tactggtaat ttaatcaata attctaagga atctttaaat
     1021 agtacctttg ataatataag attaaattta agtgaatcac aaacatcatt aaataagata
     1081 caaagtaatt tagatgaatt atcttcaata gctagtgatg caagctctgc taagattaat
     1141 tcattaataa gtaaatctat caatgaaata aataaagttg ataatagtat tacagttgta
     1201 actaactttt tagatgttat taacaaaaaa aacaacaatg aaaaaatagc taatatgata
     1261 acttcattaa aaaatattca aaattcatta gcagaagaga aaactaagct taatgattta
     1321 caaagcaaag ttaataatgg taaagattta gataaaggac ttcttagttc aataagtgat
     1381 ttaactaaga aaatagaagg gcaattagat aattctttaa atagatttga taatgataca
     1441 agacctgctt taaatacaat tgctgatgga tttgttaccg ctactaaagg ggcatcagat
     1501 ttactaggaa aagcaaatgg attggtagat caaataaata atctattaag cacagctaat
     1561 caaggggctc aacttgcaaa ttcaacatca gaaaaactaa aaaatagttt agaagaattt
     1621 tcaggagtta taagtgaatt aagttcaaag cttaaagatg ttagtgatga tgatcttggt
     1681 aaaatagttg gaatactaca aagtaatcca gaatttatgg gagatttcat tgcaaatcca
     1741 tttaatatag aagtagatgc aatatacaaa gtagctaact atggatcagg aatggcacct
     1801 atatattcgg tattagcttt atgggttggc tcattaatat taatatcatt attaaaaaca
     1861 gagtcagctg aatttgaagg tagtgagaat ataaatttaa gagaaagaca ttttggaaag
     1921 atgttaactt tcgtaagttt aggaatatta caaggattta tagtagcctt tggggataag
     1981 ttccttttag gagttcaaac agtaaataca gcattattaa tttttgtatc aatgtttgct
     2041 tcagttgtat tcactataat tgtatttacg cttatgtctg tctttggaaa cctaggtaaa
     2101 gccttagcta ttatacttat ggttttacaa ttagcaggta gtggtggatc atatcctata
     2161 caagtagatc cattattttt tagaataatt caaccaactt tcccatttac ttatgctata
     2221 tcagggtata gggaagctat tgcaggacct ttagtaagta ctgtaatttt agattttgtt
     2281 gttttaacaa taatgggatt agtatttata ttattaggat atttattgaa aggtccttta
     2341 aatcctagag ttagaaaatt tgaagatatg tttgaagaat ctggaatagc agaataagct
     2401 gtattttaag aggtgaatta tgaagaaaat ttttaaagtc tttcgaagag atttaagaaa
     2461 tataaggaaa aatccagctg ctttatcagt agtaattggt ttatgtataa taccatctct
     2521 ttatgcttgg attaatatta aggcatgttg ggatccttat gttaatacag gaaatttacc
     2581 agttgctgtt gttaataatg ataagggtgc tgagataaat ggtaaagaga taaatgtagg
     2641 taaggaagta attaatgaac ttaaaaagaa tgatgccata ggatggcaat ttgtagattc
     2701 gtggcaagga aattatgggt taaatgaagg taagtattat gcaatgattg aaataccttc
     2761 agatttctca gaaggattaa caagtttaac tacaggaaat cctcaaaagc caaatataat
     2821 ttataaagta aatgaaaaat ccaatgctat agcaaataaa ataacaaatg ttgcaaaaaa
     2881 taaagttaca gatgaaataa aatctaactt tgttgatact gtaaacaaag aagcttttga
     2941 tcttttaaat caattaggtg gaaagttaga aactaataag ccagaaatat taaatgtgag
     3001 agatacatta caaagtgcta atagtagtct aaaggaaatt caagattttg tccaaaaagc
     3061 taatggagat tcaaaaagtt taagtagtta tctttctagt gtaagaaatg atttacctaa
     3121 ggtaacagat caaataagta atttacaaac tactgttgag gcgagtaaaa gtttaataaa
     3181 ttcaacacaa caaacagtta atgcagtatc aaatagtttt agcagtgatg ttgtaacaat
     3241 gcaatcaaca aatcaggaat tacaaaattt actttcaaag cttaaagaat taaataatgc
     3301 tatggataca actaagatgg ttgaaacttt agataaatta ttaaaattaa cagattcact
     3361 aaataaaatt atagataata atattaaatc cttagaaaaa ataaattctt taacaccaaa
     3421 tgataaaata gttgatttaa taaataattt gaacaatgca aaagatttag taaacaaaga
     3481 gaaagatcaa ttagttgatt taaaaacagc attatcaaat aatgtaagca aggaagatat
     3541 agctaaagcc atagatgtaa cttctaaaat aagcaatgaa ttaagttcaa ctttaataaa
     3601 tatttcaaat aatttttata ataatggagc gccagcttta aatactattg ggaattcatt
     3661 aaagggtgga ttagatacag caaattcagt attagaaaca acaaaaatag ttgttccaca
     3721 gctaaatgct ttaactactt ttggtatagc tagtagtgat gtagctagtc aacaagctga
     3781 tcaaataaca agtaaactta atgaatttca agatgaaatc agtaaattaa tagaaaaaac
     3841 ctcaggttta actgaagaaa atttaaataa tataatagat gtaatgagta aaaatccaga
     3901 agaaatagca tcatttatat catctcctat aaaagtaaaa gaagaacagg tttatgatgc
     3961 aggaatattt ggagtaggac ttacaccatt ttatacaact ttagcaattt gggttggttc
     4021 attattactt acatcactat taacagcaga gtgtgaggat tttgaagacg gaacaaaatt
     4081 aaatcttatc caaaagcatt ttggtaaaat gttattattc ttaactatat cattaattca
     4141 aacggtaata gtagttttag gagatatata tatattagga gtaaatcctg caaatatgcc
     4201 attgatgttt ggatttgcaa tagttagttc tattgtattt acggtaatga tatttacctt
     4261 agtatcatta tttggaaaca taggtaaagc aatagccatt gttataatgg tattccaagt
     4321 agcaggtgct ggaggaattt atccaataca gacaaatcca aaaatatttg agattttaca
     4381 acctttatgg ccatttactt attctataga tggatttaga gaagctatag ctggtccaat
     4441 atggaattct gtatctaagg atatgaaagc cttaggaata ttcttattaa tattcttatt
     4501 actatgtatt cttaaaaagc cattccatag acttactgag tttgtagagc ataaatttag
     4561 agaatcagga ttataaaata aagaaaagag taaagattaa ttctttactc tttttgtttt
     4621 ataagagact cttaaaatta taattaatag aaatatgtgg taaaatataa tgtgaaaata
     4681 tttcttgtta atgtgaggtg ttttatggga agaaaagaga aaaacttata taaagcagtt
     4741 gatataaaga ataaggaaat tattgatgtt agagaaatag ggtattattc tacacctccg
     4801 tttgttgcaa gatatatagg aaaaagaatt atagatataa atggtaaagg gaaaacttta
     4861 tttgatccat gttgtggaaa agaggagcta actgattatt ttagtgattt aggaattaaa
     4921 accataggta tggatttaat aaaatataaa aataattata aatgtgagtt taagaaagac
     4981 aattttataa attattactg ctctcaaaaa aatacaaaaa catgggacta tgattattac
     5041 attgctaatc caccatataa ttgtcatgag gttaatttta taaaggagaa taaggagagg
     5101 cttaagaatt atttcaatga ggttgggttg cataatatgt acagcatgtt catgtttgcc
     5161 ataatagata aggcaaagaa tggggcgctt attggattaa ttactaatga ttcatttttt
     5221 acagcgaaaa atcataagag gcttaggaat aaaattctta gggaatgttc tatatatgaa
     5281 ataaccatgt gtcctagagg cctttttcat aatcaaggag cggatgttag gacaagcata
     5341 cttattttaa gaaagggaaa agagtatcaa gaaaagatag ttgtaaataa caggcctaaa
     5401 tccatagaag aatttaaagg gttattaaat ggggtaaaaa ataatagtaa tgaaaggctt
     5461 tatagcttac aagagattgt attgcaaaat aaaaaggata atttagagtt tttaatagaa
     5521 tgtcctgaag atataaggag tttatttaat aatattagaa ttggagaaaa gtttaaatgt
     5581 gtaacaggca tttcaacagg taatgataaa gagtttttat ctaaggaaaa aagcaatgaa
     5641 tttactattc ctttttacaa aaatccagga aaagataagt tttatacaga tagagtaatt
     5701 tatctacata aaaatttttt agaaatagaa aagattaata gaaattttaa tgttagaaat
     5761 aaagaaattc tttataaaag tggtataacc tgttcatcta tgggggtttc ctttaatgca
     5821 tgtatattgc ctaaaaactc tacttttgga gtcaatccta atataatatg tgaatcagaa
     5881 gaggtttggt ggttactagc ttatttaaat tcatctctag taacttatct tgttagggga
     5941 gcgttaataa gaagcaacat gataacttca ggctatgtat ctagaatacc acttttaagc
     6001 tttactaaag aggaaaaagc taaactttct catttagcta aaaggtctta taagttaaga
     6061 aaacaaaatt ctagtataga aactgagtta tgccatatag acaatattgt taatcatgtt
     6121 gctaaaattt caaatgagtc taaagattta atttctaatt tcaaaaagta tttagttgaa
     6181 gttacttagt ttaatctaat tttatgtgac agtacccatt aggattcttt ttaagatatt
     6241 tttgatgata ttcttcagct ttataatagt tttctaaggg cttaacctct gtcactattt
     6301 tttttgaata agatttttga agttcttctt tactatttaa aatctcttct aaatctgaag
     6361 gctctacata gtaaatgcca gttctatatt ggctaccaac atcattacct tgtttattta
     6421 atgaggttgg atctattatt gtccaaaact tagctaataa ttctttaagt gatattatgt
     6481 tttcatcata atttacaaga caaacttcag caaagtaagt atttttagtg catatatctt
     6541 cataagtagg attttcagta cgtccattag catatccaac ttcagtgctt actacaccat
     6601 tgattctaga aagaaattct tctacacccc aaaaacaacc acctgctaat acaatttttt
     6661 tcataacgaa ccctccttat aagttttagc tttcctattt ataaaataat tatgattatt
     6721 tatcacaaac tttttttatt atttttgctg gatttccacc tactactaca ttatcaggga
     6781 catccttagt tacaacggct ccagaggcta caactacatt gtttcctata gttacacctg
     6841 ggtttattgt agcatgtcct ccaatccaaa cattattacc tatggttaca ggcttaccaa
     6901 attcatattt tagtctttca ataggatcaa ttggatgagt agctgtataa atgtgaactc
     6961 caggggcaaa gaaacaattt tctcctatgg taattttaca aacatcaagc attacacaat
     7021 caaaatttgc gtaaaagtta ttacctactg atatattata accataatca catttgaagt
     7081 taggttccat atatatgctt tcacctgttg aacctaaaag ttcttttaat atactacttc
     7141 tttctttaaa agttttatca gcttcctctt cattatcagg taagtctaat ttattaaatt
     7201 tctgaaataa aattctagct ctaattctac ctttagttaa ttctgggtca gcagggttat
     7261 aaggcagccc taatatcatt ttttcttttt ctgtcatagc cataaaaaat cctccaatat
     7321 ttattctcac aattatttta tcagtaacat atatatattt catcagatta gttatagaaa
     7381 tagttaaaat ttcatataat gaatagctaa tattatattt tgaggtgtta agttatgttt
     7441 gatcctaaaa tgttaaatgt tgaatttaga aatggaatta cggatgaaaa accaatagag
     7501 ggaagaaagt atacattaac tcataatgat gatactggag agttatttct tacagtagct
     7561 ttagcatatg cttatgacaa aataaactca tcagcgagag atgaagtttt tggagtttgg
     7621 aaaagaggaa acaatagatt tatcttaaaa atatacttat atgttgatgg aaatgaagga
     7681 agagaagagg ctataaatag agatagaata tttagaaaag agttaccttt agccctagct
     7741 gccatagcat atggagatag agagttttta gatgataatg atgatttatt aaatagcaat
     7801 ataatagtga gatttatatc tgcttatgaa gagcttaata agattgaaaa ctatggaaca
     7861 cttaagaatt gtactaataa tcgcatggaa aatacaccaa acaataacaa caatatttat
     7921 attgaagaga gtattttaaa tgataatgat cctttagtaa ataattttat actaacttta
     7981 ttaaatccat atattaaagc tgaaataaca aagttatatg aaagtgatat gtattattgt
     8041 ttaaaagatg tggaagtatt agaagtattt aaagctcagg cagataatga atgtgaattt
     8101 aactataaaa tttatgttgg agttaaggta ggaactatgc caacttcata caataacctt
     8161 ataattgagt ttttagtaac tccaaatgca gtaagcactt taagtgtaaa aaatccaagg
     8221 taaataa
//
LOCUS       DAEVGI010000107         8168 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEVGI010000107 DAEVGI010000000
VERSION     DAEVGI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.107"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(488..628)
                     /locus_tag="K8M77_001877"
     CDS             complement(488..628)
                     /locus_tag="K8M77_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086362.1"
                     /translation="MVKKQANPSIIKKFESIKLGGNRLKGVFVIKQQNNKNKREPIKD
                     KM"
     gene            627..1247
                     /locus_tag="K8M77_001878"
     CDS             627..1247
                     /locus_tag="K8M77_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI7086363.1"
                     /translation="MLVYLLIYKCKKYNKNKIINKLPRKDIPKYALISIGMGGLSALW
                     LIFADTILSSITLVKNSLQNFSKISESLNNEAYIFIFLSVILFGPVVEELLFRGLIFN
                     EIDKIKGGATPIILSGLLFGLFHREPVQVVYSSILGIILGFVYSKTRSLPLVIFMHML
                     NNLVATLPPPLAKHEILQFVNGFQIISVIPMVYLLYKLYKKGSLTS"
     gene            1634..1927
                     /locus_tag="K8M77_001879"
     CDS             1634..1927
                     /locus_tag="K8M77_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086364.1"
                     /translation="MKYFIVEGILKDSDKINEDIMKEHMAYTQKAMDEGIILVSGLKS
                     DMSGGIFVIKSESVEKLEAYLSKEPFKLYGIQDYKFVEFNAHYFNPSPNEWFK"
     gene            complement(2045..3214)
                     /gene="fliB"
                     /locus_tag="K8M77_001880"
     CDS             complement(2045..3214)
                     /gene="fliB"
                     /locus_tag="K8M77_001880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HBI7086365.1"
                     /translation="MKVRVPNYFNEFKCIASECEDTCCAGWEIVIDDETHKRYEKVEG
                     EFGEILRSKIVKSDGENIFLLNNGNCSFLNEKKMCEIYINLGEDHLCYTCQQFPRYTE
                     EFLDLKEVGLSLSCPEAARIILRKAENTTFNLSEEESLTEDEDYFNEGIDAEVLLEFL
                     ECRNIVFNIIERNDLDLGTKAALALEFVKEVQNKIDLGDMDEISELMEEYRDENFINT
                     LLNELECFKGKESIKHKNLCEYLNVYKSIKHINSNDPLGLEKALKYFEGNEEFYLRKH
                     KEFNEYYKENLYKFKNILVYFIFRYFMKAIFDYDVSAKIKLAIISTLMIKELAVVRFI
                     ENNNEFTEEDMVEVSRIYSKDIEHSDENIENLQEIFETEEIFEVDEILPMLMNDF"
     gene            3837..4985
                     /gene="nanH"
                     /locus_tag="K8M77_001881"
     CDS             3837..4985
                     /gene="nanH"
                     /locus_tag="K8M77_001881"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118429947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase NanH"
                     /protein_id="HBI7086366.1"
                     /translation="MRNKNNTFEKNLDISHKPEPLILFNKDSNIWNSKYFRIPNIQLL
                     NDGTILTFSDIRYNGPDDHAYIDIASARSTDFGKTWSYDVAMKNNRIDSTYSRVMDST
                     TVITNTGRIILIAGSWNTNGNWAMTTSARRSDWSVQIIYSDDNGLTWSNKIDLTKDSS
                     KVKNQPSNTIGWLGGVGSGIVMDDGTIVMPAQISLRENNENNYYSLIIYSKDNGETWT
                     MGNKVPNSNTSENMVIELDGALIMSTRYDYSGYRAAYISHDLGSTWEIYEPLNGKVLT
                     GKGSGCQGSFIKATTSNGHRIGLISAPKNTKGEYIRDNIAVYMIDFDDLSKGVQEICI
                     PYPKDGNKLGGGYSCLSFKNDYLAIVYEANGNIEYQDLTPYYLLIDKE"
     gene            complement(5202..5948)
                     /locus_tag="K8M77_001882"
     CDS             complement(5202..5948)
                     /locus_tag="K8M77_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086367.1"
                     /translation="MRRYKMVIISIIILLLINNIMHSKVSYALDIANSKNQVLLSEIP
                     NEEIYLYALDSDEGKSVYEDILLNVKGKKKRFNWIVDSGLTFRPELILTDINNDGKKE
                     LVIIITTGHGTGINTQEVHIFKEETLEEIKVQNPFDIISKNVKTKVYKDENSVKVYIN
                     INGKESVISSKKLNELYNDQNNKKNFGDTLFFRDFLIYEVYNEKLKAIVGGDIFFWNY
                     LVNIKIEYKFESNMLVVDKIYFDVTEYFKD"
     gene            6124..6291
                     /locus_tag="K8M77_001883"
                     /pseudo
     CDS             6124..6291
                     /locus_tag="K8M77_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477367.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(6300..6614)
                     /locus_tag="K8M77_001884"
     CDS             complement(6300..6614)
                     /locus_tag="K8M77_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBI7086368.1"
                     /translation="MSMKILMIALFILLGIMLSLGKWSFLIAGFNTMTKEEKEKYDVM
                     SLCKFMGKLMFIIAFCITLFTLSDIFMMKILFNIGTVILIVSIIFTIIYANTGNPFER
                     EN"
     gene            complement(6631..6855)
                     /locus_tag="K8M77_001885"
                     /pseudo
     CDS             complement(6631..6855)
                     /locus_tag="K8M77_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459081.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(6944..7432)
                     /locus_tag="K8M77_001886"
     CDS             complement(6944..7432)
                     /locus_tag="K8M77_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2691 family protein"
                     /protein_id="HBI7086369.1"
                     /translation="MRGIVLDKGQEYYTNLRKVFKSVNNIHLEYNWLITDCCAYPEDE
                     DIEELLSREYCFISGEELDTIIKKEDFQLIWGVLGGFNKKITLEEIMKYNLPYAEDNK
                     DIWKNPVCIQHPLSEIEIISWDSDKTVLISKDENLLDSFKSNFPLSEDLEFYNRKLKN
                     KL"
     gene            complement(7612..8037)
                     /locus_tag="K8M77_001887"
     CDS             complement(7612..8037)
                     /locus_tag="K8M77_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086370.1"
                     /translation="MESKEMRLAKKAKKLIDEFERVQSVLLSGELSEQEVEIRDDGAT
                     IVLKNIMPGYSGEAMFKLTHISDVVKAYIDDMDEENDEMIKSYEVENTNWHESDLGDI
                     YREAAIEHLEKVEVVQDKVRHTYKELKKIEEKAREIEGL"
BASE COUNT         2932 a         1317 c          807 g         3112 t
ORIGIN      
        1 ttatccctaa gcctccttat aaaatccatt attcctaata acataaaaat tttagctttt
       61 ataaataata tgtaaccccc ttggtactat cagttattcc ctaattaatt ctataaatag
      121 ctaagctatt ttcccctaaa cattatgcat ataattagtt tatattccat atattatctc
      181 atctaggttc tttcccaagc taaaaattat ggaacttata gcactaatca aaatttgaat
      241 taagtattta ttataagaac ttattaaaat tattttcctt cacttcatat attaacatca
      301 tttaaaagga aatttatcat aatatcttct taacccttgc tatgtaagtt tctttaataa
      361 gaaaatttaa ggaaagattt atcacaaaag ctcattagac tattttttat aaattgaggt
      421 gaatcatgaa aaaaatcatt aaactatttc aagccatact aaacatattt atttttctag
      481 ccctatatta cattttatcc tttataggct ctcttttatt tttattattc tgctgcttta
      541 taacaaagac tcccttaagc ctattaccac ctaattttat tgactcaaat ttctttatga
      601 ttgatggatt tgcttgtttt ttaaccatgt tagtttatct tcttatatat aaatgtaaga
      661 aatacaacaa gaacaaaatt attaataaac ttccaagaaa agatattcct aaatacgcct
      721 taatttcaat tggtatggga ggcctctctg ctctatggct catatttgca gatacaatat
      781 tatcctctat tacccttgta aaaaatagct tacaaaattt ttctaaaatc tctgaaagct
      841 taaataatga agcatatata tttatatttt tatctgtaat tttatttggc ccagttgttg
      901 aagaattact ttttagagga ttaatcttta acgaaataga taagataaaa ggaggagcaa
      961 cccctataat tctctctgga cttttatttg gattattcca tagggaacct gtccaagttg
     1021 tttattcctc tatattagga attattttag gatttgtata tagtaaaact cgttctcttc
     1081 ctttagttat attcatgcat atgctaaata atctagttgc aacccttccc cctccactag
     1141 ctaagcatga aatactccag tttgttaatg gctttcaaat aattagcgta attccaatgg
     1201 tctacctatt atacaagcta tacaaaaaag gatctttaac aagctaaact tccccttaat
     1261 ttaaggttat ctataattat taatttaata tatttatgat tattactttt aagatgtttt
     1321 taaagtgtgc ataaaagcat cttatttaat gctaagttaa aattcctcga ttaataataa
     1381 aaacttttta aataacctta attcctaaaa gtacaactct taaaaacttt aagcactagc
     1441 aatcaaataa taagacttat atatggcttc ttaaattaac acaataatat atttacaatc
     1501 attttttaag tccaaacata tttaaaagaa gttctacctc taacctcaaa agtttatttt
     1561 agtaaatatt attaaatact attaattatt agtttataat atatttatag tacttaattt
     1621 tggaggataa gaaatgaaat attttattgt tgaaggtata ttaaaagatt cagataagat
     1681 taatgaagat ataatgaaag aacatatggc ctatactcaa aaggcaatgg atgaaggaat
     1741 aatattagtc tctggactta aatcagatat gagtggaggc atatttgtta ttaaatcaga
     1801 atctgtagaa aaattagaag cctacttatc aaaggaacca tttaaacttt atggaattca
     1861 agactataaa tttgtagaat ttaacgctca ctattttaat ccctcaccaa atgaatggtt
     1921 taaataactt tttaaacaaa acttaaaata aaaagagtgt tttaaattac ataaatatat
     1981 aacttaaaat actcttttct tttttatatt ttaatgctta aattttcaat atttataggt
     2041 aaatttagaa gtcattcatt aacataggta gtatttcatc cacttcaaat atttcttctg
     2101 tctcaaatat ctcttgtaga ttttctatat tttcatctga atgttcaata tctttagagt
     2161 aaatacggct aacctctacc atatcttctt ctgtaaattc attattattc tctataaatc
     2221 taactacagc aagctcctta atcattaagg tacttattat agctaacttt atctttgcag
     2281 agacatcata gtcaaaaata gctttcatga aatatctaaa tataaaatat acaagtatgt
     2341 ttttaaactt atataagttt tctttgtaat actcattaaa ttccttatgc tttcttaaat
     2401 aaaactcttc atttccttca aaatacttta atgctttctc tagccctaaa gggtcatttg
     2461 aatttatatg ctttatgctc ttataaacat ttaaatattc acatagattt ttatgtttta
     2521 tgctttcttt tcctttaaaa cattctagtt catttaaaag tgtatttatg aaattttcat
     2581 ccctatactc ttccataagc tctgagattt catccatatc acctaaatct atcttgtttt
     2641 gaacctcttt aacaaattca agagctaagg ccgcctttgt tcctaaatct aaatcattac
     2701 gctctataat attaaaaaca atattcctac actctaaaaa ctctaaaaga acctctgcat
     2761 caatgccttc attaaagtag tcttcatcct cagttaagct ctcctcttca cttaaattaa
     2821 aagtagtgtt ttcagccttt cttaaaataa ttcttgcagc ctctggacat gataaagata
     2881 gtcctacttc ttttaaatct aaaaattctt cagtatatct aggaaattgc tgacaagtat
     2941 aacaaagatg atcctctcca agattaatat aaatctcaca catctttttt tcatttaaga
     3001 aggaacagtt cccattattt aaaaggaaaa tattttcccc atcactttta actatcttac
     3061 ttcttaggat ttctccaaat tcaccctcca ccttttcata acgcttatgg gtttcatcat
     3121 caataacaat ttcccatcca gcacaacaag tatcttcgca ctctgatgct atacatttaa
     3181 actcattaaa ataatttggt actcttactt tcatcttttt cctcacagtt tataataatt
     3241 ctaagtctca ccttttaatt atatcaaaaa atataagtcc tttgtttaat accttgtatt
     3301 ttaaaacttt tagagttcac tccttctatc cccttaactt tttcttttta atcttcctct
     3361 aaactttttg tcacttctct aaaaaagatt aaaggaagga taatctaaac ttctattcta
     3421 cttctcattt tattttcctc tgcttaaatg tgaataacta taataaaagg cttattatct
     3481 ttaataactt ctctagaaat tattacttta ttaaagattc tctgaaatat aaagtttttt
     3541 tactcctatt tcctttcttt aaaaactatt gttttattaa aatctcctta aattaaagct
     3601 attaagctta aactttctta aagttataag aactaaaatt ttatagaaaa ctccaaaaag
     3661 aattatattt acattattcc atagtaaaga ttttcaaata ctgttataat ttatttgaaa
     3721 aaattctata tttttcaaga tatgaatttt taataattag gtttctaaag ctatttataa
     3781 gaaaaataac ttttaaatta atgggtaaat atgaatttat ggaggaaatt atatttatgc
     3841 gtaacaaaaa caacaccttt gaaaaaaatc tagatataag ccataaacca gaaccactaa
     3901 tactatttaa caaggatagt aacatatgga attcaaagta ttttagaatt cccaatatac
     3961 aattattaaa tgatggcaca attttaacct tttcagatat tcgttataat ggccctgatg
     4021 accatgctta tatagacata gcttctgcac gcagtactga ttttggaaag acatggagct
     4081 atgatgtagc aatgaaaaat aatcgtattg actctactta ttctcgtgta atggactcca
     4141 caacagttat tacaaataca ggtagaataa tattaattgc aggatcatgg aatacaaatg
     4201 gaaactgggc aatgactact tctgcaagaa gaagtgattg gtctgttcaa attatttatt
     4261 ctgatgataa tggattaact tggtctaaca aaatagattt aaccaaggac tcatcaaaag
     4321 taaaaaatca accaagtaat acaattggat ggctaggagg agttggctca ggtattgtaa
     4381 tggatgatgg aacaatagtt atgccagcac aaatttcctt aagagaaaat aatgaaaata
     4441 actattattc attaattatc tattcaaagg ataatggtga aacatggaca atgggaaaca
     4501 aggttcctaa ttcaaatact tctgaaaata tggtcataga attagatgga gctttaatta
     4561 tgagtacaag atatgattac tctggttata gggcagcata catctctcac gatttaggaa
     4621 gcacctggga aatatatgag cctttaaatg gtaaagtttt aactggtaag ggctctggat
     4681 gccaaggttc ctttattaag gctactactt caaatggaca tagaatagga ttaatttcag
     4741 cacctaaaaa cactaaaggt gaatatataa gagacaatat tgctgtttat atgattgact
     4801 ttgatgattt atctaaagga gttcaggaaa tatgtattcc ttaccctaaa gatggtaaca
     4861 aattaggcgg tggctattct tgtctatcct ttaaaaatga ctatttagcc attgtttatg
     4921 aagccaatgg aaatatagaa tatcaagact taacacctta ttacttacta attgataaag
     4981 aataataaaa tacccattat actaaaacaa atcaaagtaa ttaatagaat gccacaaaat
     5041 ttgagactag aagaaaattt atataaaaat aaaatttctc tagtctcatc tcatactaaa
     5101 atatttctga tttctctaga actaattaaa ataaaaatct catatttaag taggctatga
     5161 ttaggtttca tatttgaaat tttattatat aacttaacat tttagtcctt aaaatattca
     5221 gttacatcaa aataaatttt atcaactact aacatattac tttcaaattt atactctatt
     5281 tttatattaa caagataatt ccaaaagaaa atatctccac ctacaattgc cttaagcttt
     5341 tcattgtaaa cttcatatat taaaaaatct ctaaaaaata acgtatcacc aaagttcttt
     5401 ttattgtttt gatcattata taattcattt aattttttac tgctaatgac agactctttt
     5461 ccatttatat taatatagac cttaacacta ttttcatctt tatatacttt tgttttaaca
     5521 tttttagata taatatcaaa cggattctgt accttaattt cttccaaagt ctcttccttg
     5581 aatatatgta cttcttgagt atttattcct gtgccatgtc ctgtggttat tattataact
     5641 aattcttttt ttccatcatt attaatatca gttagtatca actcaggtct aaaagttaat
     5701 cctgaatcaa caatccaatt aaatcttttc tttttacctt taacattaag aagtatatct
     5761 tcatatacac tctttccctc atcagaatct aaagcatata aatatatctc ttcattaggt
     5821 atttctgaaa gtaatacttg attttttgaa ttagctatat ctaaggcata actaacttta
     5881 ctatgcataa tattatttat taaaagtaaa ataataatgc taattattac cattttatac
     5941 cttctcataa taatcacctc aaaatatttt ttgtaatact taactaaatt attctaaatt
     6001 ttctttaata caaacactct aaaaaaactt aaacaattta ataaaagttt acttatgact
     6061 taaaggattt ctctctatat aactagaaat tttactccac aaactaatta aaggagtata
     6121 actatgaaaa taggactagc atctaaagaa tttataaata aagatattga aaaaaatact
     6181 aatacaatta tagaaactat gatagaagca aaagaagaaa attacaaagg tttattatat
     6241 tgtagctata tgacaattaa tgaaaatagt gaaataataa ataattttaa aatataattt
     6301 caattttctc tttcaaaagg attccctgta tttgcatata ttattgtaaa tattattgaa
     6361 actattaata taacagttcc gatattaaat agtatcttca tcataaaaat atcacttaag
     6421 gtaaataatg ttatacaaaa agcaattata aacataagtt tacccataaa tttacataaa
     6481 gacataacat catatttttc cttttcttcc ttggtcatag tgttaaatcc agctattaaa
     6541 aatgaccatt tacccaagga taacattatt cctaataaaa taaataaagc aatcatcaat
     6601 atcttcatac tcataaaatc acctcataaa ttagatttct atttttattc catcataagc
     6661 aaactctata ccatctaatc ctttctcaag ctcaacatag tcatcataag atttccccca
     6721 atcctcttct aagtgagtta tgattaccct cttaatatta tatttatttt tcaattcttc
     6781 aatgtcactt atagtaaata attcatctct taaatggtta tcttatttca aaataaaacc
     6841 atccttataa actttattaa actagtaatc tttagaaatc tcagctatta tatgtttatt
     6901 gagactccca aaactgtatg gtgaatttaa actttcatta actctacaat ttatttttta
     6961 actttctatt gtaaaattct aaatcttcac ttaaagggaa attactttta aaactatcta
     7021 ataaattttc atctttgctt attagaactg ttttatcgct atcccatgaa attatctcta
     7081 tctctgaaag aggatgttga atacaaactg gattcttcca aatatcttta ttatcttctg
     7141 cgtaaggtaa attatatttc ataatctctt ctaaagtaat tttcttattg aacccaccta
     7201 aaacacccca aatcaattga aaatcttctt ttttaattat agtgtctagt tcttctccag
     7261 atataaagca atattctctt gataatagtt cctctatatc ttcatcctct ggataagcac
     7321 aacaatctgt aataagccag ttatactcaa gatgaatgtt attaacagat ttaaaaacct
     7381 ttcttaaatt tgtgtaatat tcttgccctt tatctaaaac tatccctctc aaaattatct
     7441 tctccttatt ttaaagtact taactctcat tattctttta tttactgtgt aaaatccccc
     7501 aaaatatatg tcctaaatgt gtatttaatt aatattgtta ttttatttat aaataaaata
     7561 aagtaaggtt catttaatat ataaccttac tttacaaata aaaactaaat cttatagtcc
     7621 ttcaatttct ctagctttct cttctatctt tttaagttcc ttatacgtat gcctaacttt
     7681 atcttgtacc acttcaactt tttctaaatg ttcaatagca gcttctctgt aaatatcccc
     7741 taaatcactt tcatgccaat tagtattttc aacttcgtaa cttttaatca tttcatcatt
     7801 ttcttcatcc atatcatcaa tgtaagcttt aaccacatca gatatatgag ttaacttaaa
     7861 catagcttct ccactatatc ctggcattat atttttaaga acaatagtag ctccatcatc
     7921 tcttatttcc acttcttgct ctgataattc tccacttaaa agaactgatt gaaccctttc
     7981 aaactcatct ataagttttt ttgctttttt agctaatctc atttcctttg attccataat
     8041 tagttcccca ctttctagtt aaaatctaca ctatataagt attattttta cttataaact
     8101 ttctttttat gtaatctaaa acttcaaaaa tacctttaaa taaatcatat attttatgat
     8161 cattttct
//
LOCUS       DAEVGI010000108         8153 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEVGI010000108 DAEVGI010000000
VERSION     DAEVGI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.108"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            204..2627
                     /locus_tag="K8M77_001888"
     CDS             204..2627
                     /locus_tag="K8M77_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HBI7086371.1"
                     /translation="MIKFDKKKKNNITIVNIYNFIRKTVYPSGEFVEDDFQTVVREIE
                     LIKQYGFPATYALKHDALMDERFVKLIKESIDEYDEVGAWFEITEELAKKSGVKWKGE
                     TPIDDHVHKGYSLGYSPEDRFKMADVYMADFKCIFGYYPKTVGSWVIDIRTLTYLKEK
                     YGVIGGAICRDQIGTDGFTLWGGYFNQAYYPSKLNEYIPAQREEMQLDLPLFRLLGPD
                     PIYNFEEGLREELKGVHTLEPAWIIGQNKEWVHWFFECLNEEETLGFSYAQVGQENTF
                     LWNTMYKGYEVQIEHIFNLEKEKKLRVETLAESALWYKKKYRLTPPTTFGAKKDWNTY
                     NMKTLWYNSRFYRSSFLFENNVLSIRDIHLFNENYESRYYDNYLDDNESVFDALPLMR
                     PHYWKNEIDERPEISFVKLKRIDVYEKIKGGTIDFKVVNEDKCEITWDLNHEESIKIL
                     CYEDKISIKFNVKGSKECMLCINTLPVLQELNLNTILCKHNDFKYKISLIKGEFSVDE
                     ESKILIIPEEGEILLDLNTICEKPKEKEFFTDEYLKNREGIDGYVPEYKRYKKGSKVK
                     LRAFRPIVNIDTLLINKPFSQEILLGKRDKKGEIRYTLDGSEPNEKSLLYKEPIVVND
                     RAFIKAKIFKEGYKPSNTVESRIYVTLPIANIKGLTKPTDHYMYNKRGVYDLIDGEKG
                     SLHYTDGKWLGYTEDMEVIVDLGEVKEIKEIMVGFLQDTRAWIYYPKFVEFYSSVDGE
                     EFTLLTREEKDKNEKREEVGKKDITSQREIKTRYIKIFAKNEEVCPDWSIMPREGPTF
                     MFVDQVTVI"
     gene            complement(2812..3621)
                     /locus_tag="K8M77_001889"
     CDS             complement(2812..3621)
                     /locus_tag="K8M77_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBI7086372.1"
                     /translation="MSIIQNMFQFEFMRNAFMAGFIISLLCPCIGLFLVLKRYSMIGD
                     TLSHSSFAGVAIGLVFGFNPLLTAFLFTTICALLIELLRNYYKKYAELSMSIILTFSL
                     GLALILVSSGKSSTTVNSFLFGSILTVSKTDLTIIALVSLLCFIGIFSLYDKLVYTTF
                     DEEGAKVSGINTKLINYIFTIIVGATISVSIRVMGILVISSIIVIPAATAMQFKKGFT
                     KSLIISMIVGVIDVLSGLLLASLFDLAPGGTIALTSVIVLILSLILNRKKL"
     gene            complement(3640..4308)
                     /locus_tag="K8M77_001890"
     CDS             complement(3640..4308)
                     /locus_tag="K8M77_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBI7086373.1"
                     /translation="MIKINNLSFSYSGKKPYQLNDINLTLPENEFISIVGENGSSKTT
                     LLKLLLGYLKPIKGNIKINLNSVGYVPQKVDNFNSQFSITVYEVLKVHLKTLKIKDKG
                     EIDRVLSAVNMLEFKDRLIGSLSGGQQQRVFIARALIGHPNILILDEPSTGIDEKTQK
                     DIYELIYKLNKEKEMTIICVEHNIRKSLKYSSYILELKDSKATLFKNNEYLKKLDEIN
                     NEFI"
     gene            complement(4381..4716)
                     /locus_tag="K8M77_001891"
     CDS             complement(4381..4716)
                     /locus_tag="K8M77_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086374.1"
                     /translation="MNKFMLRRLILGVSLFFIITFSSAFIINKIYIKQKCKDLYFATE
                     YLTTRGNLENSLLTIKNFELSFLDKDIAIVEINGLSYDKPHQSISCKAYFEKKENSVW
                     NLKEIEKLT"
     gene            4885..5283
                     /locus_tag="K8M77_001892"
     CDS             4885..5283
                     /locus_tag="K8M77_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086375.1"
                     /translation="MDFSSLVIMEKDKENGVIKGELGSYSVLEGTNFIKKLYCVDGDV
                     SLFFDTDKDVLEWEFSAIYDLFNVEALTSLGYIVEEFDEEYNPTWVVKFKFDDEHEEM
                     RAVLNEVCSIIDKQMKKVFEDIKDKEEDYK"
     gene            5304..7103
                     /gene="pepF"
                     /locus_tag="K8M77_001893"
     CDS             5304..7103
                     /gene="pepF"
                     /locus_tag="K8M77_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBI7086376.1"
                     /translation="MSEVKKLRRRDEIPESDKWRIDKIYETPAKWNEELSKLKEEAPK
                     LKDFEGKLGNKEDLKAFLLLNEKLSRKLGKLYVYAHMRSHEDTSNPEMQSLVNKIDPY
                     SAEFSSYTAYFVPEILSLKEGTIENFINEDKDLEQYKIYFEMILNEKPHILSKEVESV
                     LASVSDCLGAPESIYSMFTNSDMTFGEIVDESGRKVELTEGNYSSFIKSKDRKVREAA
                     FKLLFGTYKKYENTLATSLTSSIKNFVFESKTRKYNSSLEASLKPNNIPVEVYYNALK
                     TVDENMDALHRYVRIKKKLLNLDEIHMYDLYVPVIECKKEHLEYKDAISLVEEGLKPL
                     GKEYLDIFNEGINEGWIDVYENKGKRSGAYSWGSYDTMPYVLLNYNYELNDASTLAHE
                     MGHSIHSYYTRKTQPYIYGDYSLFCAEVASTTNEILLIHHLIEKETDKNKKLYLINQE
                     LEQIRTTVFRQLMFAEFELKTHEAIENGESLTSEVLCKMWKDINIKYFGEDMKVDEEI
                     SIEWARIPHFYSDFYVYQYATGYAAASSFANSILSKGEEAVEKYKGFLKAGGSMYPID
                     TLKMAGVDMTTSKPLKDTLDRFNELLDMLEEII"
     gene            7121..8149
                     /locus_tag="K8M77_001894"
     CDS             7121..8149
                     /locus_tag="K8M77_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBI7086377.1"
                     /translation="MSKFEQDYFNLLINNYGFYVEDLKGEQDKELWIALKTVKDDGKY
                     AVIISKSYEEEENLKIAEDYLKSLGKSYSLHNIILYKGYDRDEKKDEDFSIDENCHRV
                     IVDVQKREVLKSDRSSEPLAKILEFLLKKKEEPKVPWYKKLRCGKVTGILIGLNILAF
                     LVCLIVATALGAGFFRNIVEMNPKILYWMGAKHNNAIIFHGEYYRLVTSMFLHSGIVH
                     LLFNMYALYILGDFIERIYGAKKYLAIYFVSGIVASIFSLYFSPVMGVGASGAIFGLL
                     GAALVFAYNEKDRIGKALVTNIIVIILLNVFIGLSMSNIDISAHFGGFIAGAILGLFF
                     HNYKIIRK"
BASE COUNT         3182 a          858 c         1394 g         2719 t
ORIGIN      
        1 tttataatta atactagatt aaataataaa ttataggcta tgaagtagtg aataaaaata
       61 ttcattattt tatagctttt tttattaaat tgtatgaact tacaaaaaat aaaatataaa
      121 gaaaatctta ataaaaacta aaattttttc attgtttaaa gtatttagat tacttaaaat
      181 gggtttaaaa aggagggaat tatatgataa agtttgataa aaaaaagaag aataatatta
      241 ctatagtaaa tatatataat tttataagaa aaactgttta tccaagtggt gaatttgttg
      301 aagatgattt tcagacagta gttagagaga ttgagctaat aaaacaatat gggtttcctg
      361 caacctatgc actaaaacat gatgctttaa tggatgaaag attcgtaaaa cttataaaag
      421 aatccataga tgaatatgac gaagttggag cttggtttga gataactgag gaattagcaa
      481 agaagtcagg agttaaatgg aagggcgaaa ctcctattga tgatcatgtt cacaagggat
      541 attcactagg atacagtcca gaggatagat ttaaaatggc tgatgtttat atggctgatt
      601 ttaagtgtat atttggatac tatcctaaaa cagttggttc ttgggttata gatataagaa
      661 ctctaactta tttaaaggag aagtatggag taataggagg ggctatttgt agagatcaaa
      721 ttggaactga tggatttact ctttggggag gatattttaa tcaagcctat tatccaagta
      781 agttaaatga atatattccg gctcaaaggg aagaaatgca attggattta cctttattta
      841 ggcttttagg accagatcca atttataatt ttgaggaggg attacgagaa gagttaaaag
      901 gagttcatac tttagagcca gcatggataa ttggacaaaa taaagaatgg gttcattggt
      961 tttttgagtg tttaaatgaa gaggagacct taggattttc ttacgctcag gtaggacagg
     1021 aaaatacatt tttatggaat accatgtata agggatatga agttcaaata gaacatatat
     1081 tcaatttgga aaaggagaag aagcttagag ttgaaacttt agctgaaagt gcactttggt
     1141 ataagaagaa atatagatta actccaccta ctacctttgg tgctaaaaaa gattggaaca
     1201 cttataatat gaaaacttta tggtataaca gtaggtttta tagaagcagt tttttatttg
     1261 aaaataatgt tttaagtata agagatattc atttatttaa tgaaaattat gaatcaagat
     1321 attatgataa ctatttagat gacaatgaaa gtgtatttga tgcattgccc ttaatgagac
     1381 ctcattactg gaaaaatgaa atagatgaaa gaccagaaat aagctttgtt aaattaaaaa
     1441 gaattgatgt atatgaaaaa attaaaggag gtaccataga ctttaaagta gttaatgagg
     1501 ataaatgcga aataacatgg gatttaaatc atgaggaaag cataaaaata ctttgctatg
     1561 aagataagat aagtattaaa tttaatgtga aaggttctaa agaatgtatg ctttgtataa
     1621 ataccttacc tgttcttcag gaattaaatt taaatactat tttatgtaag cacaatgatt
     1681 ttaaatataa aatttcttta attaagggag aattctcagt agatgaagaa tctaagatat
     1741 taataattcc agaggagggg gaaatacttt tagatttaaa cactatttgt gaaaaaccta
     1801 aggaaaaaga gttctttact gatgaatatt taaaaaatag agaagggata gatggatatg
     1861 taccggaata taaaaggtat aagaaaggta gtaaggttaa acttagagcc tttagaccaa
     1921 ttgtaaatat agatacttta cttattaata aaccattttc tcaagaaatt cttttaggaa
     1981 aaagagataa aaaaggagaa attagatata ctttagatgg aagtgagcca aatgagaaat
     2041 ccttgttata caaagagccc atagttgtta atgatagagc ttttataaaa gctaagattt
     2101 ttaaagaagg atacaagcct agtaatactg tggaaagtag aatatatgta actttgccaa
     2161 tagctaatat taaaggatta actaagccta cagaccacta tatgtacaat aaaagaggag
     2221 tttatgattt aatagatggt gaaaagggaa gtttacatta tactgatgga aaatggcttg
     2281 ggtacacaga agatatggaa gtaatagttg acttagggga agtaaaagaa ataaaagaaa
     2341 taatggttgg atttttacaa gacacaagag cttggattta ctatcctaag tttgtagagt
     2401 tttattcttc tgtagatgga gaagagttta ccttattaac aagggaagaa aaggataaga
     2461 atgaaaaaag agaagaagta ggaaaaaagg acattacatc acaaagagaa attaagacca
     2521 ggtatattaa aatatttgca aagaatgaag aggtatgtcc agattggagt ataatgccta
     2581 gagagggtcc tacctttatg tttgttgatc aagttacagt aatataaaga gattaatagc
     2641 aataaaaata tagtttctaa gctatatttt tatgattttc ttttaaaaat aaaggattat
     2701 ataaaaaagc ttaataataa aacttacaaa ggatttgaag agaaatctag taattctcag
     2761 aatatgtttg taagttttat taacttttta tacaacccat ttttacttat attataattt
     2821 ttttctattt aaaattaatg ataatataag tactattact gaagttaaag ctatagttcc
     2881 tcctggagct aaatcaaata gagaagctag aagtaaaccg cttaagacat ctataactcc
     2941 tactatcatt gaaataataa gggatttagt aaatcctttt ttaaattgca tggcagtagc
     3001 tgctggtata acaattattg atgaaataac cagtattccc ataactctaa ttgaaacaga
     3061 aatagtagct cctactatga ttgtaaaaat ataatttata agttttgtgt ttattccaga
     3121 aactttagcc ccttcttcat caaaggtagt ataaactaat ttatcatata atgaaaatat
     3181 tcctataaaa caaagtagag aaactaaagc tattatggtt aaatcagttt ttgaaactgt
     3241 taatatactt ccgaataaaa aagaattaac tgttgtacta gacttaccac tactaactag
     3301 tattaatgct aatcctaaac taaaggtaag tataatagac atagataatt ctgcatattt
     3361 tttataataa tttcttaata attctataag taatgcacaa atagtagtaa ataaaaatgc
     3421 tgttaaaaga ggattaaatc caaatactag cccaattgca acgcctgcaa aggaagaatg
     3481 ggaaagtgta tcccctatca ttgaatatct ctttaacaca agaaaaagtc ctatacaggg
     3541 acataaaaga gatattataa aaccagccat aaatgcatta cgcataaatt caaattgaaa
     3601 catattttgt ataatagaca tttttaaaaa gagccctttc tatataaatt cattatttat
     3661 ttcatctagt ttttttaagt attcattatt tttaaataaa gtagctttag aatcttttaa
     3721 ttcaagaata tatgatgaat acttaagaga ttttcgtata ttgtgttcaa cacatataat
     3781 agtcatttcc ttctctttat ttaacttata aataagttca tatatatctt tttgagtctt
     3841 ttcatctatt cctgtagaag gttcatctaa tattaatata ttagggtgtc ctattaaagc
     3901 ccttgcaata aaaactcttt gttgctgacc accagaaaga gaacctatta atctatcctt
     3961 aaattctagc atattgacag ctgaaagaac cctgtctatt tcgcctttat cctttatttt
     4021 taatgttttt aaatgtactt ttaatacttc ataaaccgta atggaaaatt gggagttaaa
     4081 gttatcaact ttttgaggaa catacccaac gctgtttaaa tttattttga tgtttccctt
     4141 aataggtttt aagtatccta aaagaagctt taaaagagta gttttagaac ttccattttc
     4201 tcctactatt gatataaatt cattttcagg taaggttaaa tttatatcat ttaattgata
     4261 aggtttttta ccgctgtagg aaaaggaaag attatttatc tttatcaaat cgtatcactc
     4321 ctagtataat tctaaataaa ttatacatgg attttaaatc aagtgcaaat aatttgcatt
     4381 ttaagttagc ttttctattt cttttaagtt ccaaactgaa ttttcctttt tttcaaaata
     4441 agctttgcaa gaaatagatt gatgaggctt atcgtaacta agtccattaa tttctacaat
     4501 tgcaatatcc ttatctaaaa atgataattc aaaatttttt atggttagaa ggctattttc
     4561 taaattccct cttgttgtta aatattcagt tgcaaagtat aaatctttac atttttgctt
     4621 aatataaatt ttattaataa taaaagcact tgaaaatgta ataataaaaa atagggatac
     4681 tcctaaaata agtcttctta gcataaattt attcatgaaa atcacctcta gtatattaat
     4741 gttacataaa acatattacc ataattttac atttaaataa atataaagag gatgacttta
     4801 tttatatatg ttatatacac ttgcaaatat aaatataaat tttataatta gtatatatag
     4861 taaaagagga aaggatgata taagatggat ttttcttcat tagttataat ggaaaaagat
     4921 aaagaaaatg gagttataaa gggtgaatta ggaagttatt cagttttaga aggaactaat
     4981 tttattaaaa aattatattg cgtagatgga gatgtaagtc ttttttttga tacagacaag
     5041 gatgttttag aatgggaatt ttcagcaata tatgatttgt tcaatgtgga ggcccttact
     5101 tcattaggat atatagtgga agagtttgat gaagaatata atccaacttg ggtagtcaaa
     5161 tttaaatttg atgatgagca tgaggaaatg agagcagttt taaatgaagt ttgctctatt
     5221 atagataaac aaatgaagaa ggtttttgag gatataaagg ataaagaaga agattataaa
     5281 taatatttga aaggagaatt aaaatgagtg aggtcaaaaa acttagaaga agagatgaaa
     5341 ttccagaaag tgataagtgg agaattgata aaatttatga aactccagct aaatggaatg
     5401 aggaattaag taagttaaaa gaggaagctc caaaattaaa agattttgag ggtaagttag
     5461 gaaacaagga agatttaaaa gcttttcttt tattaaatga aaagttaagc agaaaacttg
     5521 gtaaactata tgtttatgct catatgagaa gtcatgagga cacatctaat cctgaaatgc
     5581 aatctttagt aaataaaata gatccatatt cagcagagtt ttcaagctat actgcatact
     5641 ttgttccaga gatattaagc ttaaaagaag gaacaataga aaatttcata aatgaggata
     5701 aggacttaga acaatataaa atctactttg aaatgatatt aaatgaaaag ccacatatat
     5761 tatcaaagga agttgaatct gttttagctt ctgtatcaga ttgcttagga gctccagaga
     5821 gcatatattc aatgtttact aattcagata tgacttttgg agagatagta gatgaaagtg
     5881 gtagaaaggt agaattaact gaaggaaact atagttcttt tataaagagc aaggatagaa
     5941 aggtaagaga agctgccttt aaacttttat ttggaacata caaaaagtat gaaaatactt
     6001 tagcaacaag tttaacaagt tctattaaaa actttgtttt tgaaagcaaa acaagaaaat
     6061 ataatagttc cttagaggct tctttaaaac ctaataatat tccagtagaa gtttactata
     6121 atgctttaaa aactgtagat gaaaatatgg atgctcttca tagatatgtt agaattaaga
     6181 aaaagcttct taacttagat gaaattcata tgtatgattt atatgttcca gttatagaat
     6241 gcaaaaaaga acatttagaa tataaggatg ctataagttt agttgaagaa ggattaaaac
     6301 ctttaggaaa agaatattta gacatattca atgaaggaat aaatgaaggt tggatagatg
     6361 tatatgaaaa taaaggaaag agaagtggag cttattcatg gggatcctat gatactatgc
     6421 catatgtatt attaaactat aattatgagt taaatgatgc atcaacttta gctcatgaaa
     6481 tgggccattc aatacattct tattatacta gaaagactca accatatatt tatggagatt
     6541 atagtctttt ctgtgcagag gttgcttcaa caacaaatga aattttactt atacatcatt
     6601 taatagagaa agaaactgat aaaaataaaa aattatatct aataaatcaa gagttagagc
     6661 aaataagaac aactgtcttt agacaattaa tgtttgcaga atttgagtta aaaactcatg
     6721 aagctattga gaatggagaa tctcttacat cagaagtttt atgtaagatg tggaaagata
     6781 taaacattaa atattttggt gaagatatga aggtagatga agaaatatct atagaatggg
     6841 ctagaatacc tcatttttat tctgatttct atgtatatca atatgctaca ggctatgcag
     6901 ctgcatcttc ctttgcaaat tcaattcttt caaaaggaga agaggctgtt gaaaaataca
     6961 aaggcttctt aaaggctgga ggatcaatgt atcctataga tacattgaaa atggctggag
     7021 tagatatgac aacatctaag ccgcttaaag ataccttaga tagatttaat gagcttttag
     7081 atatgttgga agaaattatt tagaagattg aggagaggca atgagtaaat ttgagcaaga
     7141 ttatttcaac ttacttataa ataattatgg tttttatgta gaagacttaa aaggagaaca
     7201 ggataaagaa ctatggatag ctttaaagac agttaaagat gatggaaaat atgcagttat
     7261 aatatctaag tcttatgaag aggaagaaaa tttaaaaatt gcagaagatt atttaaaaag
     7321 cttaggtaag tcatattctc ttcataatat aattctttat aaaggctatg atagagatga
     7381 gaaaaaggat gaagattttt ctatagatga gaattgtcat agagtaattg ttgatgttca
     7441 gaaaagagaa gttttaaaaa gtgataggag ctctgagcct ctagcaaaaa tactagaatt
     7501 tttattaaaa aagaaagaag aaccaaaggt tccttggtat aaaaaattaa gatgtggaaa
     7561 agttacagga atattgattg gtttaaatat tttagctttt ctagtttgtc ttattgtagc
     7621 tactgcttta ggtgctggat tcttcagaaa tatagtagag atgaacccca aaattctata
     7681 ttggatgggt gctaagcata ataatgcaat aatatttcat ggagaatatt atagattagt
     7741 aacctctatg tttttgcata gtggaatagt acatctttta tttaatatgt atgctctcta
     7801 tatattagga gatttcatag aaaggattta tggagcgaaa aaatatttag ctatctattt
     7861 tgtttcagga atagtagcaa gtatatttag cttatacttt tcaccagtta tgggagttgg
     7921 cgcttcagga gctatatttg gacttttagg ggcagcttta gtttttgcgt ataatgaaaa
     7981 agatagaatt ggtaaagctt tagtaactaa tataatagtt attatattgc ttaatgtatt
     8041 tatcggtcta tcaatgtcta atatagatat atctgctcat tttggtggat ttatagcagg
     8101 agctatttta ggacttttct tccataatta taaaataata agaaaataaa aaa
//
LOCUS       DAEVGI010000109         8093 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEVGI010000109 DAEVGI010000000
VERSION     DAEVGI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.109"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(99..1457)
                     /locus_tag="K8M77_001895"
     CDS             complement(99..1457)
                     /locus_tag="K8M77_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBI7086378.1"
                     /translation="MEKQNLKNTEVNLIYGVDDDLDLPKKVLFGLQHIFAAFGGIIVV
                     PLVIATSLGFDSKVTTALISASILGSGLATIIQAKGIFKVGARVACIMGTDFTFVSPA
                     ISVGSVLGLPGIIGATILGSLFEVILSFFIKPLMKFFPPLVTGTVVALIGLTLLPVSI
                     DWAAGGAGSANYASLENLAVAMFVLIITLLLNNYGKGIISSASILIGIVVGYIVCIPL
                     GLVDFTSVKEASWLSFPKILEFGVTFDAKAIMAFIPAYFVATIGTVGCLKAIGETSNI
                     DIGDKRVAAGVLSDGVGSALGGLVGSCPNTSFSQNIGIISLTKVASRHVAVMAGILLV
                     ILGFLPKVAAIITGIPNPVLGGVGIMMFGTVAAAGIRTLSNIKLTERNLLIIAISMGL
                     GLGVTFRPDVIHNLPEAIRMIFSSGISTGTIAALILNAVLKESPTSMEFENMYDEETK
                     AS"
     regulatory      complement(1716..1817)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2047..2364
                     /locus_tag="K8M77_001896"
     CDS             2047..2364
                     /locus_tag="K8M77_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086379.1"
                     /translation="MQITDKKVKNSFVIRAVKQIGTFFIFTMVFAIVFSFLQPRVYKT
                     HSEYLSIKEKYDKLQKEYDSLNEKISNDTNALNSKKENLTKSNEDLQNKLNSLNKEIQ
                     SLN"
     gene            2377..2649
                     /locus_tag="K8M77_001897"
                     /pseudo
     CDS             2377..2649
                     /locus_tag="K8M77_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2675..3208
                     /locus_tag="K8M77_001898"
     CDS             2675..3208
                     /locus_tag="K8M77_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HBI7086380.1"
                     /translation="MSDKKLFTIKTSINKKDYSRFLYIATFLRNKFTIPAIIVITALM
                     AGFVSYNNRTFELKSFLIYWLILLIITLFAIVVKIEFQSREREKADKAGVLKSIETLE
                     FFDDILVIKSTAFKGKSKIKYYKFYEVIESKNYFIIYFNRGQASIIRKVDLEDSLVDE
                     LIDLLTGKIGNKYKKLF"
     misc_binding    3355..3623
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3772..5109
                     /gene="brnQ"
                     /locus_tag="K8M77_001899"
     CDS             3772..5109
                     /gene="brnQ"
                     /locus_tag="K8M77_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBI7086381.1"
                     /translation="MKDLSRKDLLLVSLMLFSLFFGAGNLIFPPFLGQAAGSSTWITI
                     LGFFVTAVGFPVLGVIAVAKSGGLHALASRVNPVFATFFTVLIYISIGPGLGIPRAGS
                     LPFEMAVAPFLPEGLISNNLALFLYTFVFFAVAYWLSLSPAKLVDRMGKVLTPSLLVL
                     IGLIFVASIFKPLGAYGDVTGVYKESPFISGFLDGYMTMDTIAALNFGIVIALVIKSK
                     GVKEEKFVVKTSVKAGIIAGGLLIVIYSMLAHLGATSGGRFGLTENGAQTLSNIITYI
                     FGKPGAILLAVIFTLACLTTCVGLITSCSQYFQTLTKKINYLSWVRILTLTSMVLANM
                     GLNKILAVSVPILNAIYPMAIMLIVLAMLDKYIKGNSVVYTTTIAFTTIVSVIHSLSC
                     TGINIEFLNSIFENLPFYSEGLGWVLPALFGLGLGLVLKSFFEKNKEKVLEVS"
     gene            5364..6236
                     /locus_tag="K8M77_001900"
     CDS             5364..6236
                     /locus_tag="K8M77_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBI7086382.1"
                     /translation="MNKKNGKMYRVAYRITAIILFGALAGIGVNFFLTPAHIYSSGVT
                     GISQLLSSIGGDFFNINIDISTWVLILNLPLAYLSFRKLGKKFTLYSFLSIISLSFFI
                     GIIPTRAVTNNQLLNSIFGGVLMGAGVGMCFRAGFSTGGTDIIVLVVQKMTGKSVGQL
                     GFIVNGIILLVAGLVYGWELALYSLVSIYVTTKVIDLFYIQQYKLTVTIYTKKEKEIV
                     DSLIKSQMRGVTIGRNLYGGYTNEPLSSITTVISKHELLIVKKNVMDIDPEAFVNIQP
                     TVEVMGNFYDNSLI"
     gene            6363..7709
                     /locus_tag="K8M77_001901"
     CDS             6363..7709
                     /locus_tag="K8M77_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI7086383.1"
                     /translation="MEKVDFSKLTGDLVTRENIEYEKSIESWNRAIKKYPLGIVFCNN
                     IDDVKNALEWAKENNIPFRIRVGRHNYEGYSIGNDVLVIDLSKMNNIIIDEENMKVTI
                     EGGVKNEEIYEALGVLGYPFPGGGCPTVGVVGFTLGGGWGYSSRLLGLGCDNLLEVKF
                     INSKGKIVIANEYDNSDLFWATKGCGGGNFGVVVSMTFKIPQKIEMATLIDIDYPNAE
                     KEELVYVIRTLQKKFKNLDRRMNLKTAIYNSKDKGIGVKITGLFYGHKEEANEILLPF
                     KLPTRVNFNLSYMRVLEANRKIEYSHPPYEKYKSTGRFVFRDYDNSEIEKLIDIVSNR
                     AEGAYYTAISFYGLGGAVKDIHKNSSAFYYRDARFIMGIQSVWEDDIFAEENIKWIKN
                     NFKYIESITTGSFINFPFKDLKDYEEEYYGENKERLREIRKKFDENKFFFFEQIIK"
     gene            7744..>8093
                     /locus_tag="K8M77_001902"
     CDS             7744..>8093
                     /locus_tag="K8M77_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086384.1"
                     /translation="MTYKSSIILNKEKYNSYFDNTFKNSIVHHIKCLLLGVFTLGLAY
                     PWIICMKYESTCKHTVICGKRLKFIGDPKELIGHWIFWWILIVITFGLYGFVVKTKFQ
                     QWTNANTIFDDVQI"
BASE COUNT         3065 a          913 c         1326 g         2789 t
ORIGIN      
        1 tgtatattat gtaataaaaa tatatttagt ttttaattag attttattta ttaatagtga
       61 atagttaaga ttattatatt tattaacttt aaaacttttt aactagcctt tgtttcttca
      121 tcatacatat tttcaaattc cattgatgtt ggactttctt ttaatactgc atttaggatt
      181 agggcagcta ttgttccagt tgagattcca gaagagaaga tcattctaat tgcttctgga
      241 agattatgaa ttacatcagg tctaaatgta actcctagtc caagccccat agatatagct
      301 attattaaaa gattacgctc tgtaagtttt atattactta gtgttctaat accagctgcc
      361 gcaactgttc caaacatcat tatgcctacg ccacctaaaa ctgggtttgg tattcctgtt
      421 attatagcgg ctacttttgg taagaagcca aggataacta atagtatacc agccattaca
      481 gccacatgac ggctagctac cttagttaga gaaataatac ctatattttg actaaaagaa
      541 gtattaggac aagaacctac tagtccacct aatgcacttc ctacaccatc agataatact
      601 ccagcggcca ctcttttatc accaatatca atattagatg tctctccaat agcctttaaa
      661 catcctacag ttcctattgt ggctacaaag taagcaggta tgaaggccat aattgccttg
      721 gcatcaaaag ttactccgaa ttcaaggatc ttaggaaatg atagccaact tgcttccttt
      781 acagaagtaa agtcaactag tccaagtgga atacaaacta tgtatcctac aactatacct
      841 ataagtattg aagcactact tattattcct tttccatagt tattaagtaa aagagttatt
      901 attaatacaa acatagccac agctaaattt tctaaactag cataattagc tgaaccagcg
      961 ccacctgcgg cccaatcaat tgatacagga agtaaagtta atcctattaa ggcaactacg
     1021 gtacctgtaa caagtggtgg gaaaaatttc attaaaggtt ttataaagaa acttaaaata
     1081 acttcaaata aagaaccaag tattgtagct ccaattattc caggtaatcc aagaactgaa
     1141 ccaactgaaa ttgcagggga tacaaaggta aaatcagtac ccataataca ggcaactctt
     1201 gctccaactt tgaagattcc cttagcttga ataattgtag ctagtccaga accaagtata
     1261 gaagcactta ttaaggccgt tgtcacttta ctatcaaagc caagagaagt tgcaataact
     1321 aagggtacta ctataattcc gccaaaagca gcaaatatat gttgaagtcc aaagagaact
     1381 ttttttggta aatctaaatc atcatctact ccatagatta agttaacttc cgtgtttttt
     1441 aagttttgct tttccataat ttcatctcct tatagttttt tagaatttac atattaagat
     1501 ttaaatttat atccaaaata aaaattccct ataagtttta aaataaaaaa tatgagttct
     1561 attaaatgta ttttaagaga attttttatt ggaataatat actttaaatc ttaaaactca
     1621 tataaagaaa ggtttcttat aacttttagg aagttatact taataaaaaa acaacacctt
     1681 tttagaggtg ttgccttaaa actagaatta ctagaattta tagtacaact cctcgtagtc
     1741 tgctatttta cggttagcag gtagaaactt tcggaccata ttaccgatta tatacgagtt
     1801 aaaatttaat acatttatta tatcacgaaa aatttaaata tcaatagttt atattgttcg
     1861 ctatataaat ttattttatt tttaaattat ttttaattag tttaaaatag cctatttgta
     1921 gtgagctata tgaatgattt aatttgtggg aaattatgtc tataataaat ctttttaaat
     1981 tatataataa tgaaatttat gtataaatta tgataaaatg ttagtggtat tgagaggggg
     2041 ataaaaatgc aaattacaga taaaaaagtt aaaaatagtt ttgtaataag agctgttaaa
     2101 caaataggaa ctttctttat ttttactatg gtttttgcta ttgtatttag ttttttacaa
     2161 ccacgagttt ataaaacaca tagtgaatat ctttctatta aagagaagta tgataagctt
     2221 caaaaagagt atgattcctt aaatgaaaaa ataagtaatg ataccaatgc tttaaattct
     2281 aaaaaagaga atttaactaa aagtaatgaa gatttacaaa ataaattaaa ttcattaaat
     2341 aaagaaattc aaagtttgaa ttaggagtag gagaatatga taaaagaaat tataaaattt
     2401 ataaaaaatc ataaaatagc tcttatatta gcattgattt tagggatttc gcctaatctt
     2461 atggttaaag attattcaaa ggaatttaat gaagtagctc ctaaagttaa tgagttagag
     2521 tataaaatac ctgccttaaa aaataataca gagcttaaat caattcagga agaaatagat
     2581 aaattacaaa atgaaaataa caccttaagt aaagaggtag aaaataaaca aaaatattta
     2641 gataatttaa atattaataa ataggagtaa gggaatgagt gacaaaaaat tatttacaat
     2701 aaagacatct ataaataaaa aagattatag tagatttttg tatatagcaa cttttttaag
     2761 gaataaattt acaatacctg ctattatagt aattacagct ttaatggctg gatttgtttc
     2821 ttataataat aggacttttg aattaaaaag ctttttaatt tattggttaa tattattaat
     2881 cataacttta tttgcaatag ttgttaaaat tgaatttcaa agtagggaaa gggaaaaggc
     2941 tgataaagct ggtgttttaa aatccataga aactttagaa ttttttgatg atattttagt
     3001 gattaaaagc acagcattta aaggtaaaag caagatcaaa tattataaat tttatgaggt
     3061 aatagagagt aaaaactatt ttataatata tttcaataga ggacaggctt caataataag
     3121 aaaggttgat ttagaggatt cattggtaga tgaattaata gatttattaa caggaaaaat
     3181 aggtaataaa tataaaaaac ttttttagtt aaattttatt ttaaattgaa aaagaaaatt
     3241 cggtataatt tattttttat taatgtatta ttagaaaaaa taaaaaataa ataaaaaata
     3301 cacttaaggt tgacagctgg aaaaataaga gataatatac ttataaattc aaaattaaat
     3361 tctatgaaga gaaaaagtag ggcatattta ttgatccaca gagagttgac aagttgctga
     3421 aagtcaatgt tcaaggtata tctgaaaatc atctcggaga agtaaagctg aagctatatt
     3481 ttagtaagtt ttatcggatt ttcaccgtta taggaaaagc gtattattta gtacgtattt
     3541 taagagtgct ataaattaaa tctatttttt taggttatat ttatagaatt agggtggtaa
     3601 cgcgattaaa cctcgtccct tttataggga cggggtattt tttattttta aaggaggttt
     3661 tttcatggac atagaaggag aaattataat tttttatagc gattaaataa ttaaaaacta
     3721 tatttttacg gaaaaaatat agttaataaa aaagaaatga ggtcattgtt tatgaaagat
     3781 ttatcaagaa aagatttatt attagtaagt ttaatgttat tttcattatt ttttggagct
     3841 gggaatttaa ttttcccacc attcttaggg caagcggctg gaagttctac ttggattact
     3901 attttaggat tttttgttac ggcagtagga tttccagtac taggggtaat tgctgtggcg
     3961 aaatcaggag gattacatgc attagcaagt agagtaaatc ctgtctttgc aacgtttttc
     4021 acagttctta tctatatttc aataggacct ggcttaggga tacctagagc aggaagtctt
     4081 ccctttgaga tggcagttgc gccattttta ccagaaggac ttatatctaa taatttagca
     4141 ttatttttat atacttttgt attttttgca gttgcatact ggctatcttt atcaccagca
     4201 aagttagtag atagaatggg gaaagtatta actccaagtt tacttgtatt aataggatta
     4261 attttcgtag caagtatttt taaaccttta ggagcatatg gagatgttac aggagtttat
     4321 aaagagtcac catttataag tggattttta gatgggtata tgactatgga tacaatagct
     4381 gccttaaact ttggaattgt aatagcactt gtaataaagt ctaagggagt aaaagaagaa
     4441 aaatttgttg taaaaacctc tgtaaaggca ggaataattg ctggaggatt attaatagta
     4501 atatactcaa tgcttgctca tcttggagca actagtggtg gaagatttgg tttaactgaa
     4561 aatggagcac aaactcttag taatataatc acttatattt ttggaaaacc aggagctatt
     4621 ttattagcgg taatatttac attagcttgc ttaacaactt gcgtaggtct tattacatct
     4681 tgtagccaat actttcaaac tttgactaaa aaaataaatt atctttcttg ggttagaata
     4741 ttaacattaa caagtatggt tttagccaat atgggattaa ataaaatatt agcagtatca
     4801 gttccaattt taaatgcaat ttatcctatg gcaataatgc ttattgtctt agcaatgtta
     4861 gataaatata taaaaggaaa ctcagtggta tatactacaa ctatagcatt tacaacaata
     4921 gttagtgtta ttcattcttt aagttgtaca ggtattaata tagaattttt aaacagtatt
     4981 tttgaaaatt tacctttcta ttcagaggga ttaggatggg tattaccagc cctatttggt
     5041 ttaggattag gtttagtttt aaaatcattc tttgaaaaga acaaagaaaa agttttagaa
     5101 gttagttaaa taaaatagag aataattcac tttgtttcgt aaattttttc cttagataca
     5161 aataataaac tagtatcgaa tattaatatt taaacttaaa tgtatataaa ataacaaata
     5221 gatattaaaa agcactaaat atcataaatt acaaaaagag taaataaaac actttaaaag
     5281 aattttattg atgatataat agattaagca aaatcacaag caataattta caatgaaaaa
     5341 taattttgaa cggagtgaat tcagtgaata aaaagaatgg aaaaatgtac cgggtagcct
     5401 atagaattac tgctataatt ctttttgggg ctttagctgg aatcggggtt aactttttct
     5461 taacacctgc acatatttat tcatcaggtg ttacaggaat atcacagcta ttatcatcta
     5521 ttggtggaga tttttttaat ataaacattg acatttcaac atgggtttta attttaaatc
     5581 taccacttgc gtatttatca tttagaaaat taggaaagaa atttacactt tatagttttc
     5641 tttctataat ttcattatca ttttttatag ggattattcc aacaagagct gttactaata
     5701 accaactttt aaattccata tttggaggag ttcttatggg agctggagtt ggaatgtgct
     5761 ttagagcagg attttctact ggaggtacag atattatagt tttagttgtt caaaaaatga
     5821 cagggaagtc agttggtcaa ctagggttta tagtaaatgg aataatatta ttagttgcag
     5881 gattagttta tggatgggaa ttagctttgt atagcttagt ttcaatttat gtaactacta
     5941 aggttataga tttattttac attcaacaat ataaacttac agtaacaatt tatactaaaa
     6001 aagaaaaaga aattgttgat agtttaatta agagtcaaat gagaggagtt acaatcggta
     6061 gaaatcttta tggtggatat actaatgaac cattaagctc aattaccaca gtaatttcaa
     6121 agcatgaatt attaattgtt aagaaaaatg ttatggatat agacccagaa gcatttgtaa
     6181 acatacagcc aactgttgaa gtaatgggta atttctatga taattctctt atatagataa
     6241 aaaggtgaag gattaattta gttaatttaa ctaaagttat aatctttcac tttttttatt
     6301 ttataagtaa agaacttatg atatcatcat tagatagaat atttattata gaaggtgaga
     6361 atatggaaaa ggttgatttt tctaaattga ctggtgattt agtaaccaga gaaaatatag
     6421 agtatgaaaa aagtatagaa tcttggaata gagctataaa aaaatatcct ttagggatag
     6481 ttttttgtaa taatattgat gatgtaaaaa atgctttaga atgggctaaa gaaaataata
     6541 ttccatttag aataagagtt ggacgtcata attatgaagg atactccata ggaaatgatg
     6601 tattagtaat agatttaagt aaaatgaata atataataat cgatgaagaa aatatgaaag
     6661 tcactataga aggtggcgtt aaaaatgaag aaatatatga agccttaggg gttttaggat
     6721 atccatttcc aggaggggga tgccctacag ttggtgttgt aggatttact cttggtggag
     6781 gttggggtta ttcaagtaga ttacttgggc ttggatgtga caaccttcta gaagtaaagt
     6841 ttataaatag taaagggaaa attgttatag ctaatgaata tgataattct gatttgtttt
     6901 gggctactaa aggttgtgga ggtggaaatt ttggagtggt tgtttcaatg acctttaaaa
     6961 ttcctcaaaa aatagaaatg gcaactttaa tagacataga ttatcctaat gcagaaaaag
     7021 aagaacttgt ttatgtaata agaacattac aaaaaaagtt taaaaactta gataggagaa
     7081 tgaacttaaa aacagctatc tacaattcaa aggataaggg aattggagtt aagattacag
     7141 gattatttta tggacataaa gaagaagcta atgaaatatt acttccattt aaattgccaa
     7201 caagggttaa ttttaattta agttacatga gagttttaga agctaataga aagattgaat
     7261 atagtcatcc tccttatgag aaatataaat caacaggaag atttgtattt agagattatg
     7321 ataattctga aatagaaaaa cttatagata tagtttcaaa tagagcagag ggggcatatt
     7381 atactgccat atccttttat ggacttggtg gagcagtaaa agatattcat aagaattcat
     7441 cagcttttta ctatagggat gcaaggttta ttatgggaat acaatctgtt tgggaagatg
     7501 atatctttgc agaagaaaat ataaaatgga ttaaaaataa ttttaaatac atagaaagca
     7561 taactaccgg atcatttata aactttcctt ttaaagacct taaggattat gaagaggaat
     7621 attatggaga aaataaagag agattgagag aaattagaaa aaaatttgat gaaaataaat
     7681 ttttcttttt tgaacaaatt ataaaatgat tttataatat tgataaagga gaggaggatt
     7741 aaaatgacat ataaatcatc tattattttg aataaagaaa agtataactc atattttgat
     7801 aatacattta aaaattcaat agttcatcat ataaaatgtt tattgttagg agtatttaca
     7861 ttaggattag catatccatg gattatttgt atgaaatatg aatctacatg taaacataca
     7921 gttatttgtg gaaagaggct taaattcata ggtgatccta aggagttaat aggacattgg
     7981 atattttggt ggatacttat agtaataact tttggtttat acgggtttgt agttaaaact
     8041 aaatttcaac agtggacaaa tgcaaataca atttttgatg atgtacaaat aaa
//
LOCUS       DAEVGI010000110         8028 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEVGI010000110 DAEVGI010000000
VERSION     DAEVGI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.110"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            204..1214
                     /locus_tag="K8M77_001903"
     CDS             204..1214
                     /locus_tag="K8M77_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBI7086385.1"
                     /translation="MAKKTSKKSNSKKPIKNRKLIITLSIIGLLIVSLVGGVATYGLT
                     MLGKINKVDLNENNLGIDKAIDEKLSKYDGIANIALFGIDATDGNVGRSDAIMIATVD
                     KIHNKLKLTSIMRDSYVDIQGHGKDKINHAYAFGGHELALKTLNENFDLNLKDFATVN
                     FSSLPKIIDSVGGMDLNIRQDEIDASPGINDHIKDLNRLSNTDCPLITSPGVQHVNGT
                     QALAYCRIRYTAGGDGERTERQRIVLSKLFEKFNQVSPAKYPTILNELLPMVTTSLSS
                     SEILNLAKIVASMGGSGLEQERYPMDEYSKGEMIHGVYYLTFDKDATVKQIHDYIFED
                     KK"
     gene            1333..2505
                     /locus_tag="K8M77_001904"
     CDS             1333..2505
                     /locus_tag="K8M77_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HBI7086386.1"
                     /translation="MKRRRIITTLILTLTLSLQSISVFAQPNKEVTNISSEKTDINNG
                     WVHENNNWYYILNNKRITGWKEIDNNWYHFNSEGLMETGWKFINGYWYYLKSNGSMAT
                     GWQFINNDWYYLNSNGSMATGWKFINNDWYYLKSNGTMATSWQKINNNWYYLNSNGPM
                     ATGWRFLDNNWYHLSENGPMTIGWKNSDDKWYYLDNNGAMVQNSEKTVNGNIYKFDSD
                     GVMITDKWFGSTYVNKDGIVLHGTPSRSHSYTQYKLFNYMSNEDNRESVHYAAIDLHG
                     GETTNNCVYFTSEALRRAGVKIPLYVANTYQLERELLSRGWIKSTNTSDLRPGDVVFS
                     GYKHSFTFMNWYDKDYAYIVDNQKKYFDSVLHKRLVSVDDPINDTIRATHFFYLPE"
     gene            complement(2600..3283)
                     /locus_tag="K8M77_001905"
     CDS             complement(2600..3283)
                     /locus_tag="K8M77_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HBI7086387.1"
                     /translation="MRAILLAAGMGTRLRPLTLDTPKSLIEVNGRPLLERQVEFLRER
                     GVEEIIVVTGYLAEKFDYLKDKYGVKLVNNDKYDIYNNIYTMYLVREYLENTYVVDAD
                     VYISENFLLEKPETSMYFSANKQGFKGEWKLSFDENLKVNDIEVGDDEGYILCGVSYW
                     DKKDTDIIVKELEDMVENGDFSELYWDDIVKNNISKLDIRIEKISENSIYEIDNLEEL
                     DYVKSVVEK"
     gene            complement(3430..4398)
                     /locus_tag="K8M77_001906"
     CDS             complement(3430..4398)
                     /locus_tag="K8M77_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI7086388.1"
                     /translation="MLKSNYKGYGSGIISAMAWGLDTVLVGVILSMAPFINTEAAIML
                     APFVSTFLHDFFSTLWMFIYMIFTRQVGKLFKSLKTPSTKYVILASIFGGPMGMTGYL
                     LSVNYIGPSYAATISSIYPAVGAVLAAIFLKEKISRKGYIGLTISILGIALLGFGNDG
                     GKASLIGFLFAGLSVLGWGSESVICSYGMKDEDISPKQALQIRQFISALFSGVIILPL
                     LKAYPLVFEVLKGNVIFLIIGTALMGTISYVCYYTAIHKLGPTRAMGLNITYVVWAMI
                     FDKVLLGHDITIKMVICALMVMIGSFVVATQPAHEEELTNVTNTAV"
     gene            complement(4424..6292)
                     /locus_tag="K8M77_001907"
     CDS             complement(4424..6292)
                     /locus_tag="K8M77_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBI7086389.1"
                     /translation="MLSYDIEVLKIINDEDNISQRRLAEMLNLSLGKINSLLKELLTK
                     EYIVKIDIDKRNVKYELTEAGIALLENSLDKVKSTKLSIHKHKYHKVKQAVILGAGGK
                     GDFGKPAGFLELEEFRIIDRIIDILKSNGIEKIVIVNGYKKEYYEELAKKDSTIFCVT
                     NSNYKWTGTMSSLALAKEYIDDDFILVENDLVFESRAVEQIIKNDNRDCVLITNESGS
                     GDEAFVEIRDGYLFKMSKDVHQFNKIDGEMIGISKISYKLFKMMLEEFKHNINPYMNY
                     EYTMLDVARNYKVGYEKINDLVWGEIDNKDQYEKVKKHIIPMIRRKEMQYKIDQVKSA
                     IVNGLKVSEEEVKEIVPVGGMTNKNYKAFVNDKAYIVRIPGLGTSSMINRRDEMINSK
                     LAADEGIDAKILFFDEESGVKIAELIEGAETLNPATAKKKENMELVVGALRTLHNSDI
                     KMENRFNVFEKIEEYESLVKKVNGTLFEDYYEIKTRVLKLEKVLEDNGMEIKPCHNDT
                     VPENFVKDINERMYLIDWEYSGLNDPMWDLAAHSIECDFSEDDEELFLNLYFNNLIED
                     KHKIRILVYKICQDFLWSIWTILKEAQGDDFGTYGIDRYNRGKKNLELLDKILMGQ"
     gene            complement(6511..7875)
                     /locus_tag="K8M77_001908"
     CDS             complement(6511..7875)
                     /locus_tag="K8M77_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBI7086390.1"
                     /translation="MKFTKRKIKLFSLTCAITTLCSGFVLGENVQAANNKEWKFENNK
                     WSYVDSNNLRSKDWENIKGDWFYFNSKGEMEIGWKYINKTWYHLRENGVMDTGWRKIN
                     GDWYYFNNSGYMMTGWQNINGSWYHLRENGVMDIGWRELGGAWYHLNESGAMETGWKY
                     INNKWYHFKDSGVMSRKWDYINKEWYYFDNNGVMKIGWQNINDTWYHLKENGVMDTKW
                     NKINDYWYYFNDYGKMINNQYIDGWRIDNNGVAHYEKAFRNQKLENSNQIIVVTTNNM
                     STSYCNIEIYEKNDSGKWNNIDSTTGRVGANGLAYIENRVQSTNTTPAGVMSITGAFG
                     VKNNPGTKLDYIKVNNNMYWDLNSENSTYNRLINYNPGGDYEHLISYPRQYEYSLITD
                     YNHNQVPNKGGAIFVHCLGRGATGGCVSMPREKMIKILKWIDPKKNPKILVIPKDDLD
                     NYWY"
BASE COUNT         2781 a         1343 c          841 g         3063 t
ORIGIN      
        1 ctttaaattc tataaaaaag attatttttt aactataatt ttattcgctt tatcttaatc
       61 aaaaataaac tttctttaat tttaatattt taaaaataac attaatatta tttttaaatt
      121 tcaataatct tatccataac tttacaactt atgcaatgaa atgctacaat tattttgtaa
      181 ttttaaaaag gaggatgctt aaattggcaa aaaaaactag taaaaaaagt aattctaaaa
      241 agcctattaa aaatcgcaaa ttaataataa cactttcaat tatagggctt ttaatagtta
      301 gtttagtagg aggtgtagct acttacggac taactatgct tggaaaaatt aataaagttg
      361 atttaaatga aaataattta ggaattgata aagcgataga cgaaaaactt tctaaatacg
      421 atggaatagc taacatagct ttattcggaa ttgatgcaac tgacggtaat gtaggtagat
      481 cagacgctat aatgattgct acagttgata aaatacataa taaacttaaa ttaacttcaa
      541 taatgagaga ctcttatgtg gatattcaag gacatggaaa agataaaatt aaccatgcct
      601 acgcttttgg tggacatgaa ttagctttaa aaactttaaa tgaaaacttt gacttaaatc
      661 ttaaagattt tgctacagtt aacttctcat cattacctaa aataatcgat tctgttggtg
      721 gaatggactt aaatataaga caagatgaaa ttgatgcaag tcctggtata aatgatcata
      781 taaaagattt aaatagactt agcaatactg attgtccttt aataacttca cctggtgtac
      841 aacatgtaaa tggtacacaa gcattagctt actgtagaat aagatacact gctggtggag
      901 atggtgaaag aactgaaaga caaagaatag ttttaagcaa attatttgaa aaatttaatc
      961 aagtttctcc agctaaatac ccaacaatct taaatgaact tcttcctatg gtaactacta
     1021 gtctttcatc tagtgaaatt ctaaatcttg caaaaatagt tgcttctatg ggtggttctg
     1081 gtttagaaca agaaagatat cctatggatg agtattctaa aggtgagatg attcatggcg
     1141 tttattattt aacatttgat aaagatgcta ctgttaagca aattcatgat tatatttttg
     1201 aagataaaaa ataaaatttt tcatatactt ctatatattt atagaagtat tatttttgct
     1261 taatactatt gatttaaatt aaaatataat tttttaattc tataatatta atattaaaaa
     1321 ttggaggtta ttatgaaaag aaggagaatt attacaaccc ttatactaac gcttactcta
     1381 tccttgcaaa gcatatcagt ttttgctcag ccaaataaag aagtaactaa catttcttca
     1441 gaaaagactg atataaataa tggctgggtt catgaaaata ataattggta ttatatatta
     1501 aataataaaa gaataactgg ttggaaagag attgataata attggtatca ttttaatagt
     1561 gaaggactta tggaaaccgg ttggaaattt ataaatggtt attggtatta tttaaaatct
     1621 aatggatcta tggctaccgg ttggcaattt ataaataatg attggtacta tttaaattct
     1681 aatggatcta tggctactgg ttggaaattt ataaataatg attggtatta tttaaaatct
     1741 aatgggacta tggctacttc atggcaaaaa ataaataaca attggtacta tttaaattct
     1801 aatggaccta tggctactgg ttggagattc ttagataata attggtacca cttaagtgaa
     1861 aatggaccta tgactatagg ttggaaaaat tcagatgata aatggtatta tcttgataat
     1921 aatggtgcaa tggttcaaaa ttctgaaaaa acagttaatg gaaacatata taaatttgat
     1981 tcagatggtg taatgataac tgataaatgg tttggctcta cttatgtaaa taaagatgga
     2041 attgtattac atggtactcc atctagatca cattcatata ctcaatacaa gttatttaat
     2101 tatatgagta atgaagataa tagagaatct gttcattatg ctgctataga ccttcacggt
     2161 ggagaaacaa ctaataactg tgtttatttc acttctgaag ctttaagaag agctggagtt
     2221 aaaatccctc tatacgtggc taacacatat caactagaaa gagagttact ttctagagga
     2281 tggataaaat caactaacac aagtgatctt agacctggag atgtagtatt ttcaggatat
     2341 aaacattcat ttacatttat gaattggtat gataaggatt atgcttatat agttgataat
     2401 caaaaaaaat attttgattc tgttcttcat aaaagactag tctcagtaga tgatcctatt
     2461 aatgatacta taagagcaac tcatttcttt tacttaccag aataatatat ataaatttac
     2521 ttttaattta taaaattatt agtattatat aaaaaggaga caagctatta gctaaactta
     2581 agctaatagc ttgtctttat tatttttcta ctactgattt aacatagtct aattcttcta
     2641 aattatcaat ttcataaata ctattttctg aaatcttttc aatgcgaata tctaacttag
     2701 aaatattatt ttttacaata tcatcccaat ataattcact aaaatcacca ttttcaacca
     2761 tgtcttcaag ttccttaact attatatctg tatctttctt atcccaataa gatactccac
     2821 ataatatata accttcatca tctccaactt ctatatcatt tacctttaaa ttctcatcaa
     2881 aactaagttt ccattctcct ttaaagcctt gtttattggc agaaaaatac attgatgttt
     2941 ctggtttttc taataagaaa ttttctgata tataaacatc agcatcaacc acataggtat
     3001 tctctaaata ttctctaact aaatacatag tatatatatt gttatatata tcatatttat
     3061 cattattaac tagttttact ccgtatttat cctttaaata atcaaatttt tctgcaagat
     3121 aacctgtaac tacaattatt tcttctactc ctctttctct taaaaattct acctgtcttt
     3181 ctaaaagagg tcttccatta acttctataa gtgacttagg tgtatctaaa gttagtggtc
     3241 ttaatctagt tcccattcct gctgctagta atattgctct catctataaa tctcctttat
     3301 tattctctca aaacttactt tatatttctt tttctttaaa ataatctaat attaaggcat
     3361 ttacaatccc ctcttaatta gtaaaatata attgatttct tttattatat atttcatatc
     3421 tattaagaac tacactgctg tattagtaac attagttaat tcttcttcat gtgctggttg
     3481 agttgccact acaaatgagc ctatcataac cattaaagca catattacca ttttaatagt
     3541 tatatcatga cctaatagta ctttatcaaa aatcattgcc catacaacat aagttatatt
     3601 aagtcccatt gctcttgttg gtcctaattt atgaatagcc gtataataac aaacgtaaga
     3661 gattgttccc attaaagctg tgcctattat aagaaaaata acatttcctt ttaaaacttc
     3721 aaatactaat ggataagctt ttaataaagg aagaattatt actcctgaaa aaagagcaga
     3781 tataaactgt ctaatttgta aagcttgctt aggacttata tcttcatctt tcataccata
     3841 agagcatatt acagattctg aaccccatcc taaaacactt aacccagcaa ataaaaatcc
     3901 tattaaacta gcttttcccc catcatttcc aaatcctaaa agagctattc ctaatatact
     3961 tattgttaat ccaatatacc cttttctact aattttttct tttaagaata ttgctgctaa
     4021 aacggctcca acagcaggat atattgatga tatagtggct gcatatgatg gtcctatata
     4081 gttaacagaa agtaaatatc cagtcatacc cataggccca ccaaatatag atgctaaaat
     4141 aacatatttt gtacttggag tttttagtga cttaaaaagt tttccaactt gtcttgtaaa
     4201 aatcatataa ataaacatcc ataaagttga aaaaaaatca tgtaaaaatg tacttacaaa
     4261 aggagctaac attatagctg cctctgtatt tataaatgga gccatggata atataactcc
     4321 aactaaaaca gtatctagcc cccaagccat agcagatata attccactgc catatccttt
     4381 ataattactt ttaagcatac tgcccctcct ttaaattcat actttattgc cccattaaaa
     4441 ttttatctaa taattctaaa tttttctttc ctctattata tctatctata ccataagttc
     4501 caaaatcatc gccttgagct tcttttaaaa tagtccatat actccataaa aagtcctgac
     4561 atattttata gacaagaatt cttatcttat gcttatcttc aattaaattg ttaaagtata
     4621 gatttaagaa taattcttca tcatcctctg aaaaatcaca ttcaattgag tgagcagcta
     4681 aatcccacat aggatcattt aatccactat actcccaatc tattagatac attctttcat
     4741 taatatcttt aacaaagttt tctggaacgg tatcattatg acatggtttt atttccattc
     4801 cattatcttc taaaactttt tctaacttta atactcttgt ttttatttca taataatctt
     4861 caaaaagagt accgttaacc tttttaacaa gactctcata ctcctcaatt ttctcaaaaa
     4921 cattaaatct attttccatt tttatatctg aattatgtaa agttcttaat gctcctacca
     4981 ctaattccat attttctttc tttttagctg ttgcaggatt aagagtttct gctccctcta
     5041 taagttctgc aatctttact ccagactctt catcaaagaa aagtatttta gcatctattc
     5101 cttcatctgc tgctagttta gagtttatca tttcatctct tctattaatc atagagcttg
     5161 tacctaatcc tggtattctt actatataag ctttgtcatt tacaaaagcc ttatagtttt
     5221 tattagtcat acctcctaca ggtactattt ctttaacttc ctcttctgaa acctttaatc
     5281 cattaactat agcacttttt acttgatcta ttttatattg catttctttt cttcttatca
     5341 taggtataat gtgtttcttt actttttcat attgatcttt attgtcaatt tctccccaaa
     5401 caaggtcatt tatcttttca taaccaacct tataatttct agcaacatct aacatagtgt
     5461 actcataatt catatatgga tttatattat gtttaaattc ttctaacatc attttaaata
     5521 atttataaga tattttgctt ataccaatca tttctccatc aatcttatta aattggtgta
     5581 catcttttga cattttaaaa agatagccat ctcttatttc aacgaaggcc tcatcccctg
     5641 atccactttc attagtaatt aaaacacaat ctctattgtc atttttaata atttgttcta
     5701 cagctctact ttcaaaaaca agatcatttt ctaccaatat aaaatcatca tctatatatt
     5761 ccttagctaa agctaaagaa ctcatagttc ctgtccattt ataatttgaa ttagttacac
     5821 aaaaaattgt tgaatctttt ttagctaatt cttcatagta ttctttctta tatccattta
     5881 ctataactat tttctctata ccatttgatt ttaaaatatc aattattcta tcaattattc
     5941 tgaattcctc aagctctaaa aaaccagctg gctttccaaa atcaccttta ccaccagctc
     6001 ctaaaataac agcttgttta actttatgat atttatgctt atgtattgat aactttgtac
     6061 tcttaacttt atctaatgaa ttttctagta atgcaattcc tgcctcagtt aattcatatt
     6121 taacatttct cttatctata tcaattttaa ctatgtattc tttagtcaat agttccttaa
     6181 gtaaagaatt tattttacct aaagaaagat ttaacatttc tgctaatctt ctttgactta
     6241 tattatcttc atcatttata atctttaaaa cttcaatatc atatgataac atcctcaact
     6301 ctcccttcgt tcattttttg aacattaata taatatcaca aaattttcct tattacaata
     6361 aaaaaggggc tgtatcaaaa tataataaaa cttactttga tacacgccct tcttaaaact
     6421 atttaataat attaatttta aaattactat ttaaatttga tattattgct agatatatac
     6481 ttcttattta agattatttt atattaagta ttaataccaa taattatcca aatcatcttt
     6541 aggaataact aaaattttag gattcttctt aggatctatc cactttaata ttttaatcat
     6601 tttttctcta ggcattgaaa cacatcctcc agtagcccct cttcctagac aatgtacaaa
     6661 aattgctcct cctttatttg gaacctgatt gtgattataa tcagttatta aagaatattc
     6721 atattgtcta ggataagaaa ttaaatgctc ataatctcca cctggattat aattaatcaa
     6781 tctattatat gtagaatttt cgctatttaa atcccaatac atattattat taacctttat
     6841 ataatctaat tttgttccag gattattctt aactccaaaa gcgcctgtta tgctcatgac
     6901 accagctgga gtagtattag ttgattgaac tctattttca atgtaagcaa gtccattagc
     6961 tcctactctt ccagttgtac tatcaatatt attccatttg ccagaatcat tcttttcata
     7021 aatttctata ttacaataac ttgtactcat attatttgtc gttacaacta ttatttgatt
     7081 actattttct aacttttgat ttctaaaagc tttttcataa tgagctaccc cattattatc
     7141 tattctccat ccatcaatat attggttatt tatcattttc ccataatcat taaagtaata
     7201 ccaataatca tttattttgt tccattttgt atccataact ccattttctt taagatgata
     7261 ccatgtatca tttatatttt gccaaccaat tttcataact ccattattgt caaaataata
     7321 ccactcttta tttatataat cccactttct actcattaca ccactatctt taaagtgata
     7381 ccatttatta tttatatatt tccatccagt ttccatagca ccactttcat ttaaatgata
     7441 ccatgctccc cctagttctc tccatccaat atccattaca ccattttctc ttaaatggta
     7501 ccaactgccg ttaatatttt gccaaccagt catcatgtat ccactattat taaaataata
     7561 ccaatctcca tttatttttc tccaaccagt atccattacg ccattctctc tcaaatgata
     7621 ccaagtttta tttatatact tccatcctat ttccatttct ccctttgaat taaagtaaaa
     7681 ccaatccccc tttatattct cccaatcctt acttctaaga ttattagaat caacatagct
     7741 ccacttatta ttttcaaatt tccattcctt attattagct gcttgtacat tttctcctaa
     7801 tacaaatcca ctacataaag tagtaattgc acaagttaaa ctaaataatt ttatttttct
     7861 ttttgtaaat ttcattatag actcccctta caataattat taaaacatga aaaattaatt
     7921 atatattcat aattttaaca taaaataaat gctttgttaa ttattcttat aaccatttat
     7981 ctaaattatg tataaattaa aatttaaaca acataaaaaa actatatg
//
LOCUS       DAEVGI010000111         8011 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEVGI010000111 DAEVGI010000000
VERSION     DAEVGI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 8011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8011
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.111"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            163..477
                     /locus_tag="K8M77_001909"
                     /pseudo
     CDS             163..477
                     /locus_tag="K8M77_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(585..983)
                     /locus_tag="K8M77_001910"
     CDS             complement(585..983)
                     /locus_tag="K8M77_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HBI7086391.1"
                     /translation="MRKKYFILTILTMILGLLSRRFMSYIPSLISLYLGDILWATMVY
                     FGFRFLFPRLSTNTSFLIAIIFSFGIEFSQIYQAKWINNIRATTLGGLVLGHGFLWSD
                     LLCYTIGILIGYFIDRLAFIKKSPKVSKKP"
     gene            1301..1975
                     /locus_tag="K8M77_001911"
     CDS             1301..1975
                     /locus_tag="K8M77_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBI7086392.1"
                     /translation="MTDRVECKLKAKELLRGRWENAVGACLLFFVSTFLLSFLLSPIP
                     IFGWIIIALISTFLTGSFIKYCIKLNETEGKVKYLGCLISFKTTLKILLCDILIGLVV
                     GIGGVMFTMIFSVASIVSQSIVIIIVALIILVALGFFIEAILFPVPMILTEDEGVGVM
                     EAIEMSFNITKGYRWKYIVMNLSFIGWMILSFLTFGIGMLWLQPYMMLTYYLFYKGIR
                     SANTTI"
     gene            complement(2168..2791)
                     /locus_tag="K8M77_001912"
     CDS             complement(2168..2791)
                     /locus_tag="K8M77_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7086393.1"
                     /translation="MEFLGSLLTFLINIVGSFGYFGILLIVGLEYACFPLPSEVILTF
                     FGLSASQGEISLIGVIIFSIIGGLLGSLICYAIGYYGGVPLQEWLGRKFPSSQKSMLA
                     LNKWFKKYGKPAVLLARIVPLTRTYVSLLAGAEKFSLKYFILYSSLGISIWNIFLVSL
                     GYFIGDNLPLINSIMEKYTFVTLAILIIAVIIFIYLKFFKKEKNTSK"
     gene            complement(2888..5545)
                     /locus_tag="K8M77_001913"
     CDS             complement(2888..5545)
                     /locus_tag="K8M77_001913"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBI7086394.1"
                     /translation="MKKVYSKSKEELFKELGVNKDGLNDTEVKSMEEKYGKNELVEGK
                     KKSTFQVFLDQFKDMLIIILICAAAISMFLGDIESAAVIIFVLIVNATLGTIQHLKAE
                     QSLSSLKALSSPSTKVLRNGERIEIDSKDVVVGDIIFLEAGDYVPADGRILENYSLQV
                     NESSLTGESESIVKMSEIIKGEEIPIGDRKNMVFSGSLVTYGRATILATAVGMDTEIG
                     KIAQLLNDTKEKKTPLQANLDNFGKKLAIGILVICAIVFALSLYRGEAIIDAFMFAVA
                     LAVAAIPEALSSIVTIVLSLGTQKMAKENAIIRKLQAVEGLGSVSIICSDKTGTLTQN
                     KMTVKKIFVDGKVIDSNELNLEKKIDNDLLTMSLLCNDAITDCEKEIGDPTEVALVNL
                     GDLYRVDELVQRDKFKRLGEIPFDSDRKLMSVVYNIHGENIIATKGAVDVLLKRSTKI
                     ETSEGVRPITKEDIAKIEKINQEFSKTGLRVLSFAYRNIESRTDIKLDDENDLTFIGL
                     ISMMDPPREESAEAVKACIEAGIKPVMITGDHKITASAIAKQIGILKDESEAIEGSAL
                     DKVSDEELKNMVEHISVYARVSPEHKIRIVRAWQEKGNVVAMTGDGVNDAPALKQADI
                     GVAMGITGSEVAKDAASMVLTDDNFATIVKAIENGRNIYSNIKNAIKFLLSGNTAGIL
                     SVLYASILALPVPFAAVHLLFINLLTDSLPAIALGLEPHKKEVMKEKPRKANASILTK
                     DFVTDVLFEGVVITIVTMIAFHIGLNQGGPLLASTMAFATLCLSRLVHGFNSKSKKHV
                     VSKELFSNKYLWAAFGIGFVLLNIVLLVPAFEGLFQVAPLTLNNLLTIYGLSLCTFIV
                     VQFVKFIREKFTENKTNTAKESNKENRAA"
     gene            6082..6882
                     /locus_tag="K8M77_001914"
     CDS             6082..6882
                     /locus_tag="K8M77_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HBI7086395.1"
                     /translation="MMNSHEIDFELFGDDMQFVEIELDPRETVVAEAGAMMMMDSSIE
                     METIFGDGSNSSGSGGFFGKLGGAAKRVLTGESLFMTAFTNMGVGREKVAFASPYPGK
                     IIPVDLRNYGGKLICQKDAFLCAAKGVSIGIDFRRKLGTGFFGGEGFILQKLEGDGMA
                     FIHAGGTIVRKRLLPGQKLKVDTGCLVAMTKDVNYDIEYVKGIKNAVFGGEGIFFASL
                     VGPGEVWIQSLPFSRLASKVVSSAPQVPGSRDVGEGSVLGEFGRLFQD"
     regulatory      7100..7202
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7438..8010
                     /locus_tag="K8M77_001915"
     CDS             7438..8010
                     /locus_tag="K8M77_001915"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI7086396.1"
                     /translation="MEALKEKILKEGKVREGNILKVDCFLNHQMDIKFLNEVGKEFRK
                     RFEGEKVDKILTIEASGIAIAGIASQYFDYVPVVFAKKTESLNLDKDVYESNVHSFTK
                     KKDYKVRVGKQFLNKGERVLIIDDFLAQGCATKGMIDLVEQAGAELVGIGIVIEKGFQ
                     DGRKVLEDLGVRVESLAIIDKLEDNKVYFK"
BASE COUNT         2872 a         1030 c         1280 g         2829 t
ORIGIN      
        1 tttagggaaa taatttgtat tttaaaagaa attaaagaaa aagatgtatt ttaaatttat
       61 tagatataat agcaaagatg caaggtattg gagttataaa tatatattgt gatgatatat
      121 tagtaagagt tcaaataaca gtataacctt taaataggtt taacttttat tagagattta
      181 aggttaaatc agtatatatc aagttataaa ttatcggaat ttagaaactc tactgttata
      241 gcaaagggta atgataaatt tgaacaatta gataatgatt taaagtttga atatatggaa
      301 tctgttagaa aaaacataat ttctattgtt tcatataatt atggaattgg cgatggtgga
      361 tatattgaaa ttcatgagat gatttcagat atgaaagtag aagttgatgt tggagaagat
      421 aagtatgtaa ctaaaggaag cactttgaag ttaaatggag aggtactata taaataggaa
      481 tattttaaga aagatttgaa tataagataa taaatagctt aaagatagaa acttaaagct
      541 atttatcaca tagtggttgc tattgtagat acctcttctg ggctctaagg ttttttgctt
      601 accttagggc tttttttaat aaaagcaagc ctgtctataa aatatccaat taatattcct
      661 atggtataac aaagtaaatc actccaaagg aaaccatgtc caagaactaa gccgccaagg
      721 gttgtagctc taatattgtt aatccacttg gcttgatata tttggctaaa ttctattcca
      781 aaggagaaaa taatagcgat taaaaaagaa gtattagtac ttagtctggg aaatagaaat
      841 ctaaagccaa aataaaccat agttgcccaa agtatatctc ctaaatagag tgatattaaa
      901 ctagggatat aggacatgaa ccttctagat aaaagtccaa gaatcatagt taaaatggtt
      961 aatataaaat atttttttct cataaattta tacctttcat taaattatat tttaataaaa
     1021 tttttataga aatttttatt aacattagtt tgattaagtt cttttaaatc atgatttaat
     1081 aaggaggtaa actttataat tagtaataga ttcaaaaggt ttattaccta aatcatagtt
     1141 ttatagtaat cctattgttt aaattatatc agataataaa gtgaaattta tattttaata
     1201 tattccttaa atttttgtgt taattcttaa aggatatgaa taatataaaa gtaattagat
     1261 gtctttaact aagtgataaa tttttaggag gcgctatcta gtgactgata gagtagaatg
     1321 taagctaaaa gctaaagaat tgttaagagg aagatgggaa aatgctgtag gagcatgttt
     1381 gttatttttt gtttcaacat ttttattaag ttttttatta tcgccaatac ctatattcgg
     1441 gtggattata attgcactaa tctcaacatt tttaactgga agctttataa agtattgtat
     1501 taagctaaat gaaacagagg gaaaagtaaa atacttagga tgcttaataa gttttaagac
     1561 tactcttaaa attttattgt gcgacatttt aattggatta gttgtaggaa taggtggggt
     1621 tatgtttaca atgatatttt cagttgcttc tatagttagt caaagcatag taataattat
     1681 tgtagctctt attattttag ttgctttagg attttttata gaggcaatat tattccctgt
     1741 acctatgata ttaacagaag atgagggtgt tggagtaatg gaagctatag aaatgagctt
     1801 taatataact aagggatata gatggaagta tatagttatg aacttaagct ttataggatg
     1861 gatgatatta tctttcctta cttttggaat aggtatgctt tggttacaac catatatgat
     1921 gttaacttac tatcttttct ataaaggaat aagaagtgct aacactacta tttaataaaa
     1981 atatacaaag gaataaaaat aaattttatc ataattgata aaactattat ttaattaaag
     2041 aaagattaaa tgaataataa gtagtgaaat agataattta gaataaaagt tgagattgtt
     2101 atttaattaa aaaaagactg tgctaaaact aagaaataag attttagcat agtcttttta
     2161 attataatta ttttgatgtg ttcttttctt ttttgaaaaa tttaagataa atgaaaataa
     2221 tcacagcaat aattaaaata gctaaagtga caaaggtgta tttctccatt attgaattta
     2281 taagtggtag attatcacct ataaaatatc ctaaggaaac taaaaaaatg ttccaaatac
     2341 ttatacctaa agaagagtat aagatgaaat attttaagct gaatttctca gcgccagcta
     2401 ataaagatac atatgtacga gtaagtggaa ctatccttgc tagcaataca gctggctttc
     2461 catatttttt aaaccattta tttagtgcta acatactttt ttgactgctt ggaaatttac
     2521 gtcctaacca ttcttgaaga gggacaccac catagtagcc aatggcgtaa cagattaatg
     2581 aacctaataa tccacctata atactaaata ttataactcc aataagtgaa atttctcctt
     2641 gagatgcact aagaccaaag aaggttaaaa tcacttcact aggtaatggg aaacaagcat
     2701 attctaaccc aactattaat aagattccaa agtatccaaa agaacctaca atattaatta
     2761 aaaatgttaa tagagatcct aaaaactcca tgcaaaataa tcctcctgtc tattcttaat
     2821 tggtattatt agatataaat atagaccatg ctaataccaa attggcatgg tctatagaaa
     2881 actataatta agctgctcta ttttctttat ttgactcttt agcagtgttt gttttgtttt
     2941 cagtaaattt ttctcttata aatttaacaa attgaacaac tatgaaagta cataatgata
     3001 atccatatat agttaataag ttattaagtg taagtggagc aacttggaat aatccttcaa
     3061 atgcaggtac taaaagaaca atgtttaata gtacaaatcc tataccaaat gcagcccata
     3121 aatatttatt ggagaataat tcttttgata ctacatgttt tttagattta ctgttaaatc
     3181 cgtgaacaag tcttgataaa cataaagtag caaaggccat tgtacttgct aatagaggtc
     3241 caccttggtt taatccaata tggaaggcta tcatagtaac aatagtaata actacacctt
     3301 caaataaaac atctgtaaca aagtcttttg ttaatataga agcattagct tttcttggtt
     3361 tttctttcat aacttctttt ttatgaggtt caagacctaa agcaattgca ggtaaactat
     3421 cagttaaaag gtttataaat aataagtgta ccgcagcaaa tggaactggt aatgctaata
     3481 tggaagcata taatactgat aatataccag cagtattacc agataataag aatttaatag
     3541 cattcttaat atttgagtaa atatttctac cattttcaat agcttttact atggttgcaa
     3601 agttatcatc tgttaagacc atagaagcag catctttagc aacttcagag ccagtaatac
     3661 ccattgcaac acctatatca gcttgtttta aagcaggggc atcatttaca ccatcaccag
     3721 tcatggcaac tacattgcct ttttcttgcc atgctctaac tattcttatt ttatgctctg
     3781 gagaaaccct agcatatact gaaatgtgtt ctaccatatt cttaagttct tcatcactaa
     3841 ctttatctaa agcagaacct tcaatagctt cactttcatc ttttaatata ccaatttgtt
     3901 ttgctatggc ggaagctgtt atcttatggt caccagttat cattacaggt ttaattccag
     3961 cttctataca agcttttacg gcttcagcag attcttctct tggtgggtcc atcattgata
     4021 taagacctat gaaggttaag tcattttcat catcaagttt tatatctgtt ctactttcaa
     4081 tatttctata tgcaaaagat aaaactctaa gacctgtttt tgagaattct tggttaattt
     4141 tttctatttt agctatatct tctttagtta taggtctaac cccttcagaa gtttcaatct
     4201 tagtagatct tttaagaaga acatcaacag ctcccttagt agctataata ttttcaccat
     4261 gaatgttata aacaacactc ataagttttc tatctgaatc aaaaggaatt tcacctagtc
     4321 ttttaaattt atccctttga acaagttcat ctactctgta taaatcacct aagtttacaa
     4381 gggcaacctc agttggatca cctatttctt tttcacaatc tgtaattgca tcattacaaa
     4441 gtaaactcat agttaataaa tcattatcta ttttcttttc taagtttaat tcatttgaat
     4501 ctattacttt tccatcaaca aaaatctttt taacggtcat tttattttga gttaatgtac
     4561 cagttttatc tgaacatata atagatacac tacctaagcc ttcaactgct tgtaattttc
     4621 ttatgattgc attttcttta gccatttttt gagttcctaa agaaagaact attgttacta
     4681 tagaacttaa agcttctggg atagcagcta ctgctaaggc aactgcaaac atgaatgcat
     4741 ctataatagc ttcacctcta taaagactaa gagcaaatac tattgcacag ataacaagta
     4801 ttccaattgc aagcttttta ccaaagttat ctaagttagc ttgaagagga gtcttctttt
     4861 ccttggtatc atttaataac tgagctattt tacctatttc agtatccata ccaacggctg
     4921 ttgctaaaat agtagctcta ccataagtta ctaagcttcc agagaagacc atattttttc
     4981 tatcaccaat agggatttct tctcctttta ttatttcaga cattttaact atgctttcag
     5041 attctccagt taatgagctt tcattaacct gtaaactata gttttcaagg attctaccat
     5101 cagcaggaac atagtctcca gcttcaagaa agataatatc tccaacaact acatcctttg
     5161 agtctatttc tattctttcc ccatttctaa gcaccttagt agaaggagag gagagagctt
     5221 ttaagctact taaagattgt tcagctttaa gatgctgaat agttccaaga gtagcattta
     5281 ctattaatac gaatatgata acagcggcac tttcaatatc tcctaaaaac attgaaatag
     5341 cggcagcaca tataagaatt attattagca tgtccttaaa ttgatcaaga aatacttgga
     5401 aagtagattt tttcttacct tcaactaatt catttttacc atatttttct tccatgcttt
     5461 taacttcagt gtcatttaaa ccgtccttgt tcacaccgag ttctttaaaa agttcttctt
     5521 ttgattttga ataaactttc ttcatattat tgtctccttt ctttaatata aattattgat
     5581 taaggataat ttctaacaaa taatatctat gtataggagg caaaaaaata agactcctaa
     5641 tataatctaa ggaatagtac ataaaatacc ttaaattcct tagattatat taaaagtctt
     5701 gtaaccaaat tggtgtataa taccaggtaa cttttcgtta accgcgatgt tgatattata
     5761 acttgcaact actccctctt aatacaatag atattaagta ttattaagtt taatatcata
     5821 ttatatctct ttgcgttgtc tgtcaataag tttttattac gaaaatacta tgattttatt
     5881 aagattttga aaaccctctt aataaataat atgagcttat ataaagatgt gtgaacaaag
     5941 gctttagtaa ttacgaaaag aataataagg gctttatttc ttagaatatt tttattgtag
     6001 aaggtatata ttctatgata gtatgtaaaa taaaattatg atttagtgtt ggaatagatt
     6061 tatttattgg aggatgtgta tatgatgaat tcacatgaaa ttgactttga actttttgga
     6121 gatgatatgc aatttgtaga gattgaatta gatccaagag aaactgttgt tgccgaggct
     6181 ggagcaatga tgatgatgga tagtagcata gaaatggaaa ctatttttgg tgatggaagc
     6241 aatagtagtg gatcaggtgg attttttgga aagttaggag gagctgctaa aagggtttta
     6301 acaggtgaaa gcttatttat gactgccttt accaatatgg gagtaggaag agaaaaggtt
     6361 gcctttgctt caccatatcc gggaaagata attccagtag atttaagaaa ttatggtggt
     6421 aaacttatat gccaaaagga tgctttttta tgtgcagcta aaggagtaag tataggaatt
     6481 gattttagaa gaaaattagg aactgggttc tttgggggag aaggatttat acttcaaaaa
     6541 cttgagggag atggtatggc ttttattcat gctggtggta ctattgttag aaagagactt
     6601 ctaccaggac aaaaattaaa agtggataca ggatgcttag ttgctatgac aaaggatgta
     6661 aattatgata tagagtatgt taagggaata aagaatgctg tctttggagg agaagggatt
     6721 ttctttgcaa gtttagtagg accaggagaa gtttggattc aaagtttacc atttagtaga
     6781 cttgcatcta aggttgtttc gtctgcacca caagtgccag gatctagaga cgttggcgaa
     6841 ggtagcgtat taggagagtt tggacgatta ttccaagact agattttaaa taagattata
     6901 tataataatt atattttaaa attatcagag caagttttaa agttttcatt tttagaaaat
     6961 ataaaattag taatgtttca tttctaagaa gtaataaact aaataggtta tttaatgttt
     7021 cttatgttca ttaaaatttt tttagtgata aaactttaca ttgaaagaat aagtagttat
     7081 aatactattt aataacttaa tatgtactta tataagtata tcgtatatgc tcgacgatat
     7141 gggttgagtg tttctactag gaggccgtaa acatcctaac tacgaatata taggtgattt
     7201 ctatattttt ttgtgagatt attatatatt cctaggcttt ttggcttagg aattttttta
     7261 attcatataa aattactatg taaaagcttt aataaagctt tctttttaat atgctttata
     7321 gaaattatct ttgtagtaaa gtagcctcac atagagcttt aatagattca attagaaaat
     7381 tctaaacaga gttatttatt ataatgaaat aatttttata gcagggggaa aaggaagatg
     7441 gaagcattaa aagaaaagat attaaaagaa ggaaaagtca gagagggaaa catacttaaa
     7501 gttgattgtt tcttaaatca ccaaatggat ataaaattcc ttaatgaggt tggtaaggag
     7561 tttagaaaaa gatttgaagg tgaaaaagta gataaaatac ttacaataga agcttcagga
     7621 atagctattg caggaattgc atctcaatac tttgattatg ttccagtggt ttttgctaaa
     7681 aagactgaaa gtttaaattt agataaagat gtttatgaat caaatgtaca ttcattcaca
     7741 aagaaaaaag attacaaagt tagagttgga aagcaattct taaataaagg tgaaagagtt
     7801 ttaataattg atgacttctt agctcaagga tgtgcaacta aaggaatgat agacttagtt
     7861 gagcaagctg gtgctgagtt agtaggaata ggaatagtta tagaaaaagg attccaagac
     7921 ggaagaaaag ttttagaaga tttaggggta agagttgaat ctcttgcaat aatagataaa
     7981 ttagaagata ataaagttta ttttaaataa t
//
LOCUS       DAEVGI010000112         7982 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEVGI010000112 DAEVGI010000000
VERSION     DAEVGI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.112"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..344
                     /locus_tag="K8M77_001916"
     CDS             <1..344
                     /locus_tag="K8M77_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086397.1"
                     /translation="LSIIDRIKAKGASIKSIKDTWLDTSSDNPYNSFLLTVMSGLSQL
                     ERDLISQRTKEGLRSAKTRGRNGGRPSKRNDKADTVGLLYREGYKIVDIVKQTGLSRA
                     TVYRTLKDLNI"
     gene            448..1245
                     /locus_tag="K8M77_001917"
     CDS             448..1245
                     /locus_tag="K8M77_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta2-toxin"
                     /protein_id="HBI7086398.1"
                     /translation="MKKFSSKIILGLLFICILAIGTINPIKVSAKDINPYKTIMEEYL
                     NAFKNFDIDTIVKISKDKRVNDEEQYREMLEEFKNDPNQQLKYFEIINSERVYNQEIF
                     NVKTEFINGAVYDMKFTVSPENGNLIVSDMEREKIENEGTYIVTPSMRAQVCTWDSTM
                     SAKMGSDVDYRYSSLFTYYADVITLNFRQYATSGSRSLSVKYSVVDHWAFGDDYKASE
                     WVYGENRDYADQIKIRIAPGQSFYKYRIKIENYSPASIRVFGEGYCY"
     gene            complement(1259..1903)
                     /locus_tag="K8M77_001918"
     CDS             complement(1259..1903)
                     /locus_tag="K8M77_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086399.1"
                     /translation="MSINKALNRKLKKLDISKKEFIELKNQLLDNADELKEEFLKEGL
                     SEEEAEKKVLESIELDELIKSFKENSIKKYFLSNKIIVISLTIIFSLFILKCTTFTAP
                     NRGNLSDFAYIPFRFLFNLIININNYHAWHDAFILDQIFILLLFVIFGTLIPIILNKW
                     NSLIPNLKIYIFFNVIVALLSKIRFFNFDLTILRILACILGFYIFKYLIKKLGD"
     gene            complement(1916..2230)
                     /locus_tag="K8M77_001919"
     CDS             complement(1916..2230)
                     /locus_tag="K8M77_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086400.1"
                     /translation="MDREILKGSLEIILLSLLKNKPMYGYEISKTIKSLTENSLIIGE
                     GTLYPALKRLEEKNLIENYFIELDTSKKKRKYYKITQLGLNELNLKLIDFLLIYKLIN
                     NC"
     gene            2452..3702
                     /locus_tag="K8M77_001920"
     CDS             2452..3702
                     /locus_tag="K8M77_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7086401.1"
                     /translation="MGNALQVNKENYIDDVHSESKGWITRSVIDKKGYSQWHYKYAEL
                     KDTDMSGENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVLMNLEKNYF
                     NQSIPIPNYVIDSGRGMYLIWLINKVPSQALALWKAIQEYLYNQLKCFGADRQALDAT
                     RILRVPGSINSKSKTVVNILDEYKYVYDLREIQNEFLPELKPYEKKKGRPSKINYIYR
                     ERSLYYGRIQDIIKLCELREYDLKGHRELILFLYRYYLCSFTEDVEKALNDVLELNSM
                     FRQALSEREVIRATRSAERCYLDKNKQYKYKNETLIELLEITEEEQKYMTIIISKEEY
                     KRRDRVYHKKNYDSEKAKKIYQEKLKSQGKLTKKEELEIVRNKIKALRLKGFKNKRIM
                     QMLGMSSTTTFERHISYLKKNGLL"
     gene            4024..4329
                     /locus_tag="K8M77_001921"
     CDS             4024..4329
                     /locus_tag="K8M77_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBI7086402.1"
                     /translation="MATKVIKDDVIRVRVTKEQKEKLKKIAKEKNTTISEILNVATKN
                     VIKNYEEQEKNYKKMCERSLATEEKIQEIKLNLEKRKLKNEKNFANKLKLFFPRLRK"
     gene            4590..4907
                     /gene="mobC"
                     /locus_tag="K8M77_001922"
     CDS             4590..4907
                     /gene="mobC"
                     /locus_tag="K8M77_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HBI7086403.1"
                     /translation="MNENRKREKQIKFYVNEKEYDQIKKKVEKSKLKQQEYLIKSALN
                     KKIIVIDGLKEILLELSREGNNLNQIAKKINEGEQRDIKEMKNKLNNLWNLIEKILKE
                     SNK"
     gene            4907..5995
                     /locus_tag="K8M77_001923"
     CDS             4907..5995
                     /locus_tag="K8M77_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBI7086404.1"
                     /translation="MAVIKYLPGKKSLKSQLKYLEKEGKTLEELKTGINCTSDNIEKE
                     FNIVKELYNKKEGKQYYHYTQAFNPEDKITPEKAHEIGKEWIEKNIKGYQIYLVTHID
                     KEHIHNHFIINSVSFEDGKKLQISPKKLEKMKKESNKICEREHLTEINLNKKNEVLKT
                     DEEYRIEKRGKETWKGELREVIELELKKSKSLEEFRENLKEKYNVETRVTKSTISYKH
                     PEQKKSVRGKRLGEKYTKERIINEFNKQTDRSISKGDNRGREEERGIEEGNRGAEKTK
                     GRSEEYKRRLISEGGISDRIRKDDEKYKTDGIEYFERLKKDRELAERRERELRELEEE
                     RIRREKEEYRRFEESLRRDRDNEREFEM"
     gene            5973..6710
                     /locus_tag="K8M77_001924"
     CDS             5973..6710
                     /locus_tag="K8M77_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBI7086405.1"
                     /translation="MKESLKCKLEYIENKSIELEKIELFNNAEEIRKNLDDIFDERKK
                     EYLKFFDSERKRIESENNKNKKELEELKNKIEEKRNYFRKPILILSAICIGISFYNLF
                     KTNSVSNLINEHEKKLTYKGAVEKANNKDKEIRYGKIGEWVEEGNFKIKVNSLKKESG
                     TMGKEKDIINITVENISNEDMKIDPTMFNVVGENGSKTKQALTFDDKITQIVNRGRKA
                     TFDYEVYPEEQDINLELEFGRAVIKLV"
     gene            6779..7735
                     /locus_tag="K8M77_001925"
     CDS             6779..7735
                     /locus_tag="K8M77_001925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086406.1"
                     /translation="MWNFSDEFNLRDISGHADLEFIDINLEKDTKLFLDPLLIESNGD
                     EWCKESNMILNNYFDNVFEAYKNNQKDLLYELFSFGREPNETFLGLSECEPKGKGNSA
                     DGLMKVFEDILNRGLMEDSLIKKPMDLCVFVHDFAEDGMSDLVTNVLRKKLNEFTLAQ
                     CEKYSIPVSDNKVEVGASWNSATQSWETVIDNVLLYEGKNILLVPKNIVRKSYVYSVE
                     KYLNKKILEHRQRYHLQNNTSLTTLRKYKNGNEELMPPSKKTLRKVEMKDTDSKEFVT
                     EYTKKNPKLIEEFRDEMYSKSNLGKYNLQDEELDEIVYGEDN"
     gene            7913..>7982
                     /locus_tag="K8M77_001926"
     CDS             7913..>7982
                     /locus_tag="K8M77_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7086407.1"
                     /translation="MLVGYARVSTEEQSLNRQIDMLV"
BASE COUNT         3543 a          640 c         1353 g         2446 t
ORIGIN      
        1 tgcttagcat tatagataga ataaaagcta aaggagccag tataaaaagt attaaagata
       61 cttggttaga tacatcaagt gataatccat ataatagttt tttgttaact gtaatgagtg
      121 gattaagtca gttagaaagg gacttaataa gccaaagaac taaagaaggg ttaagaagtg
      181 caaaaactag ggggagaaat ggaggaagac cttctaagag aaatgataaa gctgatacag
      241 tagggttact atatagggag ggttataaga tagttgatat agtaaagcaa acagggctaa
      301 gtagagcaac tgtatataga actttaaaag atttaaatat ttagttaaga actattatat
      361 tgtaaaatta tatatagtat gtattacttt caaaaaaatg aaaaatttgt tataataaat
      421 tggaaaaaag ttttaggagg ggaatttatg aagaaatttt catcaaaaat tattttgggg
      481 ttattattta tttgcatact tgctataggt acaataaatc caataaaagt tagtgcaaaa
      541 gatataaatc cttataaaac tataatggaa gaatatctta atgcatttaa aaattttgat
      601 attgatacta ttgtaaaaat atcaaaagat aagagagtaa atgatgaaga acaatatagg
      661 gaaatgttag aagaatttaa aaatgatcct aatcaacaat taaaatattt tgagattata
      721 aattctgaaa gagtttataa tcaggaaatt tttaatgtga aaactgaatt tattaatggt
      781 gcagtttatg atatgaaatt cactgtatca cctgaaaatg gaaatttaat agtttcagac
      841 atggagagag aaaaaattga aaatgaagga acatacatag taactccttc aatgagagct
      901 caagtttgta catgggatag tactatgagt gctaaaatgg gaagtgatgt agactataga
      961 tattctagtt tatttacata ctacgcagat gttataacat taaattttag acaatacgct
     1021 acttcaggct caagatcatt aagtgtaaaa tatagtgttg tagatcattg ggcttttggt
     1081 gatgactata aagctagtga gtgggtgtat ggtgaaaatc gtgattatgc agatcaaata
     1141 aaaatacgta tagcaccagg acaatctttc tataaatata gaataaaaat agaaaattat
     1201 agtccagcat caattagagt ttttggggaa ggatattgtt attaaaaaag ttaggtgatt
     1261 aatcacctaa cttttttatt aaatatttaa atatataaaa acctaaaata caagctaata
     1321 ttcttaaaat tgttaaatca aaattaaaaa atctaatttt tgataataaa gcaacaataa
     1381 cattaaaaaa aatatatatt ttcaaattag gaattaaaga gttccatttg tttaagataa
     1441 tagggattag cgttccaaat attacaaaca ataataatat gaatatttga tctaaaataa
     1501 aagcatcatg ccaagcatga taattattaa tgtttattat taaattaaat aaaaatctga
     1561 aaggaatata agcaaaatct gataagtttc ctctatttgg tgctgtaaaa gtagtacatt
     1621 ttaatataaa taaagaaaaa attattgtta aagatatgac tattatttta tttgataaaa
     1681 aatatttttt tattgagttt tctttaaagg atttaattag ttcatctaac tctatagatt
     1741 caagaacttt tttttcagct tcttcttcag ataaaccttc ttttaaaaat tcttccttta
     1801 attcatcagc attatctaat aattggtttt ttaactctat aaattctttt tttgatatat
     1861 ctaatttttt tagtttacga tttaaagctt tgtttatact cataaaaacc tccttttaac
     1921 aattattaat taacttatag attaataaaa aatctataag ctttagattg agttcattta
     1981 atcccagttg agtaatttta tagtattttc ttttcttttt tgaggtatca agttcaataa
     2041 aataattttc aattaaattt ttttcttcta gtctttttaa tgcgggatat agtgttcctt
     2101 ctcctattat taaagaattt tctgttaagc tttttatagt tttagaaatt tcatatccat
     2161 acataggttt attctttaat agagataaca atattatttc taatgagcct ttaagtattt
     2221 ctctatccaa taaatcaact cctttttagt taacttatat ttgtaattta attttatata
     2281 aaaatacatc gtaaagcaag gtgtttttat ataaattatt tagatttcaa aaaaataaaa
     2341 atattataaa aattggattg aaatttgatt ttttaagaaa aagttttaat gattaagtaa
     2401 tagaaaatat gatataattt tcttagtttt tacaaaaggg gaggtgaatt agtggggaac
     2461 gcattacaag tgaataagga aaactatata gatgatgttc atagtgagag taaaggatgg
     2521 ataactagga gcgttataga taaaaaagga tatagtcaat ggcactataa atacgctgaa
     2581 ttaaaagata ctgatatgag tggcgaaaat atctatataa ctttaaatac gttctataag
     2641 ccttgtagaa ggctagaaaa cataaaggaa ttaaatacat tatttataga cttagattat
     2701 tataaaactg gtaaaactaa ggatcaggta ttaatgaact tagaaaagaa ttattttaat
     2761 caaagtattc ctataccaaa ctatgtaata gatagtggaa gaggaatgta tttaatttgg
     2821 ctaataaata aagtacctag tcaagcatta gcattatgga aggcaattca ggaatattta
     2881 tataatcaat taaagtgttt tggagcagat agacaagcct tagatgcaac caggatatta
     2941 agagtaccag gaagtataaa ttctaaatct aaaacagtag taaatatatt agacgagtac
     3001 aaatatgttt atgatttaag agaaatacaa aatgaatttt tacctgaatt aaagccatat
     3061 gaaaagaaaa aaggaagacc cagtaaaata aattatattt atagagaaag aagtttatat
     3121 tatggaagga tacaagacat aataaaactt tgtgaactaa gggaatatga tttaaaagga
     3181 catagggaac ttatattatt tttatataga tattatcttt gtagctttac agaagacgtt
     3241 gagaaggcgt taaatgatgt tttagaactt aatagtatgt ttagacaagc tttaagtgaa
     3301 agagaagtta taagagcaac taggagtgct gaaagatgtt atttagataa aaataagcaa
     3361 tataagtata agaatgaaac tcttatagag ttattagaaa ttacagaaga agaacaaaaa
     3421 tatatgacaa taataatttc taaagaagaa tataagaggc gagatagagt ttatcataaa
     3481 aagaattatg atagtgaaaa ggcaaaaaaa atttaccaag agaaattaaa gtctcaaggt
     3541 aaattaacaa aaaaagaaga attagaaata gtaagaaata aaattaaagc acttagatta
     3601 aaaggcttta aaaataaaag aataatgcaa atgttgggta tgtccagtac aacaacattt
     3661 gaaaggcata tatcttactt gaaaaaaaat gggcttttat aaaagctcat tttttattgt
     3721 tttcgaattc atttttgatt ataaaacaaa ataaaaataa tttcaatttg tgggttaaga
     3781 aaaaaatacc cccatttttt ttctcggctt gtattatagg gtacttcgta cctgttcttt
     3841 tttattggag aggtttagag ataagttttt actttaggta gttccgaagg aactgttatt
     3901 agagtgcccc tttatttttt ggcgtagctt gttatgtaaa aagtattatc gaaaataaag
     3961 gtttattggt gtagctacag aattaaatta gtagaagtat ttgtttaaag tgaggatttt
     4021 tttgtggcta caaaggtaat aaaagatgat gttataaggg ttagagtaac caaagagcag
     4081 aaagaaaaat taaaaaagat tgctaaagaa aagaacacta cgataagtga aattttaaat
     4141 gtagctacaa aaaatgtgat aaaaaattac gaggaacaag aaaaaaatta taaaaaaatg
     4201 tgcgaaagat ctttagctac agaagaaaaa attcaagaaa ttaaattgaa tttagaaaaa
     4261 agaaaattga aaaacgaaaa aaattttgca aataaattaa agcttttttt tcctagatta
     4321 agaaaataaa aattaattat tagtattaaa aaaatgatta atttaatttt taggaagaaa
     4381 aaaaagcttc tctaatctac acttgaaaac tcttttctta aaggataaaa tttaattaaa
     4441 gtaagcattg caagattagt atttgagtac cctaaaaggg catatcaaat acgaccgtaa
     4501 aacaagtttt actatcttgc ttgggttagg gcaaagccct ccaagaccac acccaacaaa
     4561 aatcaaagat ttttttagga gaaaaaataa tgaatgaaaa tagaaaaaga gaaaagcaaa
     4621 taaaatttta tgttaatgaa aaagagtacg atcagataaa aaagaaagta gaaaaatcaa
     4681 aattaaaaca acaagaatat ttaataaaga gtgcattaaa taaaaaaata attgtaatag
     4741 atggattaaa agaaatactt ttagagttat caagagaagg aaataattta aatcaaatag
     4801 caaaaaaaat aaatgaaggt gaacaaagag atataaaaga aatgaaaaat aaattgaata
     4861 atttatggaa tttaatcgaa aaaattttaa aggagagtaa taaataatgg cagtaataaa
     4921 atatttgcca ggtaaaaaaa gtttaaaaag tcaattaaaa tatttagaaa aagagggaaa
     4981 aactttagaa gaattaaaaa caggaattaa ttgtacaagt gataatattg aaaaagaatt
     5041 taatatagtt aaagaacttt ataacaagaa agaaggaaaa caatattatc attacacaca
     5101 agcttttaat ccagaagata aaattacacc agaaaaagcc cacgaaatcg gaaaggaatg
     5161 gatagaaaaa aatataaaag gatatcaaat ttatttagta actcacatag ataaagaaca
     5221 tattcataat cattttataa ttaattcagt tagttttgag gatggaaaaa agcttcagat
     5281 aagtccaaaa aaattagaaa aaatgaaaaa agaaagtaat aaaatttgtg aaagagaaca
     5341 tttaacagaa ataaatttaa ataaaaaaaa tgaagtttta aaaacagatg aagaatacag
     5401 aatagaaaaa agaggaaaag aaacttggaa aggagagctt agagaagtaa tagaattaga
     5461 attaaaaaaa tcaaaaagtt tagaagagtt tagagagaat ttaaaagaaa aatataatgt
     5521 ggaaacaaga gtaacaaaaa gtacaataag ttataaacat ccagagcaaa aaaaatcagt
     5581 aaggggaaaa agattaggag aaaaatacac aaaggagagg attataaatg agtttaataa
     5641 acaaacagac aggtcaatca gcaagggaga taatagagga agagaagaag agagaggaat
     5701 tgaagaggga aacagaggag ctgaaaaaac taagggaaga agtgaagagt ataagagaag
     5761 acttatatct gaagggggga tttcagacag aattagaaaa gatgatgaaa aatataaaac
     5821 agatggaatt gaatattttg aaagacttaa aaaagataga gagttggcag aacgaagaga
     5881 aagagaactt agagagcttg aagaagaacg tattagaaga gaaaaagaag agtatagacg
     5941 atttgaagaa agtcttagaa gagacagaga taatgaaaga gagtttgaaa tgtaaattag
     6001 aatatataga aaataaaagt attgaattag aaaaaataga attatttaat aatgctgaag
     6061 aaataagaaa aaatttagat gatatttttg atgaaagaaa aaaagaatat ttaaagtttt
     6121 ttgatagtga gagaaaaaga attgaaagtg aaaataataa aaataaaaaa gaattagaag
     6181 aacttaaaaa taaaatagaa gaaaaaagaa actattttag aaagcctatt ttaatattaa
     6241 gtgctatatg tataggaata tcattttata atctatttaa aactaatagt gtaagtaatt
     6301 taataaatga acatgaaaag aaattaactt ataaaggagc agtagagaaa gcaaataata
     6361 aagataaaga gataaggtat ggaaaaatag gcgaatgggt tgaagagggt aattttaaaa
     6421 taaaagtaaa tagtttaaaa aaagaatcag gaacaatggg aaaagagaaa gatattatta
     6481 atataacagt tgaaaatata tccaatgaag atatgaaaat agatcctact atgtttaatg
     6541 tggttgggga aaatggaagt aagacgaaac aagcactaac ttttgatgat aaaataacac
     6601 aaatagtaaa tagaggaaga aaagcaactt ttgattatga agtatatcct gaagaacaag
     6661 atattaattt agaattagag tttggaagag ctgtaattaa gttagtttaa tattttatag
     6721 taataaatgg tataatataa ggtatattgt aaattttttt tacagaggag gaatatggat
     6781 gtggaatttt tcagatgaat ttaatttgag ggatattagt ggacatgcag atttagaatt
     6841 tatagatata aacctagaaa aagatacaaa attattttta gatccattat taatagagag
     6901 taatggggat gaatggtgta aagaatcaaa tatgatatta aataattatt ttgataatgt
     6961 ttttgaagca tataaaaata atcaaaagga tttattgtat gaattattta gttttggaag
     7021 agaaccaaat gaaacttttt tagggctatc agaatgtgaa ccaaaaggaa aaggcaatag
     7081 tgcagatggg ttaatgaaag tatttgaaga tatattaaat agaggattga tggaagatag
     7141 tttaataaaa aaacctatgg atttatgtgt atttgttcat gattttgcag aggatggaat
     7201 gtcagattta gtaacaaatg ttttaagaaa aaaacttaat gagtttactt tggctcaatg
     7261 tgaaaagtat agtataccag ttagtgataa taaggttgag gtaggagctt cttggaattc
     7321 agctactcaa tcttgggaaa ctgtgataga taatgtatta ctttatgaag gtaagaatat
     7381 actattagtt cctaaaaata tagttagaaa aagttatgtg tattcagtag agaagtattt
     7441 aaataaaaag atattagaac atagacagag atatcattta caaaataata cttcgttaac
     7501 tacattaagg aaatataaaa atggtaatga ggaactaatg ccacctagta aaaaaacatt
     7561 aagaaaggtt gagatgaaag atactgatag taaagaattt gtaacagagt atactaagaa
     7621 gaatccaaag ttaatagagg aatttagaga tgaaatgtat tcaaagtcaa atttaggtaa
     7681 gtataatctt caagatgaag aattagatga aatagtatat ggagaggata actgatttta
     7741 gattaataat agtatcaaaa atggtgattt ttgatacata aatttttcgt actgaaaaaa
     7801 gccaaatttt ctatgtaaaa atgtattaaa aaaggtatca taatctaaat agattatgat
     7861 acctttttgg tataataaag gaaataaatt tagaaaaaat ggagataaaa ttatgttagt
     7921 tggatatgca agggttagta ctgaagaaca aagtttaaat agacaaatag atatgttagt
     7981 tg
//
LOCUS       DAEVGI010000113         7979 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEVGI010000113 DAEVGI010000000
VERSION     DAEVGI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.113"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(105..713)
                     /locus_tag="K8M77_001927"
     CDS             complement(105..713)
                     /locus_tag="K8M77_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HBI7086408.1"
                     /translation="MKLIWKGKFNDVEDLPIGELPENAVRFEEPESAEELAKETRRFL
                     IPVVIFFLIVIFLRIKTNGFFGVSDVLNIFGIILIPFSILPHEYLHAIFFPKYAEVEM
                     WYSIKQRLALVTSNTAITKKRFIFLSIFPNIVFGFLPLIIWIFIPSDMSFISGILFTF
                     GFISLTLGSGDFMNIYNTIKQVPKDAMVQISGLNSYWFFKEK"
     gene            1283..1771
                     /locus_tag="K8M77_001928"
     CDS             1283..1771
                     /locus_tag="K8M77_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7086409.1"
                     /translation="MSIEIYEIESFKDLNLFWNMFHDYINYIFEMSPLSKETFEYFLS
                     KEYKDSVLELSERDFNPLKIIFFKKKDKILGFATYIIYIDESGKSIILEYCLNKDFRN
                     KGLGKIAYNKLQEKMIKEGAKTIELTPTNKRNELFWKNNGFSDTRIFLQDGRNLLIKK
                     LR"
     gene            complement(2419..4074)
                     /gene="hcp"
                     /locus_tag="K8M77_001929"
                     /gene_synonym="priS"
     CDS             complement(2419..4074)
                     /gene="hcp"
                     /locus_tag="K8M77_001929"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI7086410.1"
                     /translation="MENSLMFCYQCEQTVGGKGCTKRGVCGKTPEIANLQDLLIYQLK
                     GISCYAKEILDKGGNIDQEVVKFVENGLFTTLTNVNFDPEVHINLLKKSQKIKEKLRK
                     IALKGEYPEHAIYNLSDSKDEMLKDAIKAGIMYDDSLDQDIRSLRSTILYGLKGIAAY
                     GHQARFINFIDKEVDNFYFKALEATTNDKLSSEDLIELTMKTGNIAIAVMKLLDDANT
                     SKYKNPTPHKVNVTLKKGPFIIVSGHDLRDLEMLLEQSEGKGINIYTHGEMLPAHGYD
                     GLKKYKHLVGNFGSAWQNQQKEFDDIPGCILMTTNCLMQPRESYKDRIYSTSVVGWEG
                     IKHIKADENGYKDFSEIINKALELGGYDEDEECKEILVGFGHKATLSNAEKIVSAVNE
                     GNIKHFFVIGGCDGARPGRNYYTDFAKLVPKDCIILTLACGKYRFNKLEFGEVAGLPR
                     LLDIGQCNDLYSAVKIATSLADAFDTNVNSLPLSIILSWYEQKAVADLLALLALGIRG
                     IYLGPSLPAFLSPNVLQYLVDNFDLKPISTPDEDLKSCLRQNL"
     gene            4823..5089
                     /locus_tag="K8M77_001930"
     CDS             4823..5089
                     /locus_tag="K8M77_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086411.1"
                     /translation="MLKAILKIMKISNEFSSSTIAKELNISETMVEIYKEQLEQKGYI
                     RKISLSGCSQEQCSSCGCGCGSSKSLNSISGWEITEKGLLVLQK"
     gene            5139..5357
                     /locus_tag="K8M77_001931"
     CDS             5139..5357
                     /locus_tag="K8M77_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBI7086412.1"
                     /translation="MDTLEKKQVGEKLVVKGIQKDSIVKRRLLDMGITPGVTLEITGK
                     APLGDPIEVIVRGYKLTLRKNEAKSILV"
     gene            5388..5609
                     /locus_tag="K8M77_001932"
     CDS             5388..5609
                     /locus_tag="K8M77_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBI7086413.1"
                     /translation="MIPMSLTKEGSSIRIEKMLINDSLGKRLMEIGISKGNIIDVIKN
                     DTTGLIIGISGSRLALDKTLANKIFVKEI"
     gene            5824..7833
                     /gene="feoB"
                     /locus_tag="K8M77_001933"
     CDS             5824..7833
                     /gene="feoB"
                     /locus_tag="K8M77_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBI7086414.1"
                     /translation="MLTIALAGNPNCGKTSLFNLLTKSRQHIGNWPGVTVEKKEGTLK
                     FKGESYKVIDLPGTYSLGAYSEDEIVARNYILKDKPDVVINVVDATNLERNLYLTTQL
                     IEMGANVVIALNMIDQAESLNIEIDTNKLSKRLGVPIIKTSAFKNRGIEELIETSIHS
                     KKNEKLININYGEDIENEIKNLSYLLEAYKNKLEFPVNWTALKLLENDEYIKDKVKQL
                     NSPSIFTKLEESNKTIEKNIGFEADMSIVDKRYSFISSITEDVIKKPSKKQVTTTEKI
                     DKIVTNKYLGIPIFALIMYCLYELTFIIGAGIQEWFGDLIAKAGVIVSDFIANMGAPE
                     LLVGFINDGLFGGVGAVLSFLPLIMVMYFLLGLLEDSGYMARAAYVMDRLMRGLGLHG
                     KTFVSMIVSVGCNVPGIMSTRTLENKKDRMIAILINPFISCGARMPIYAVFVEAFFPT
                     HQGLVLFSLYVLGIIVALISGKIFSKTLFKGESSYFVMELPAYRIPSIKNVFLLMWEK
                     AGAFFKKAGMIIFPMMIVLWALSVLPLGVEPNSEHSILGMIGSFVAPLFVLAGYGTWQ
                     AGVSLITGILAKESVVATMGMVYAGVEEGEALINVIQQVFTPLSAVSFLVMTLLYTPC
                     LAALGVIKRETNSMKWTIFSAVYTFVIALVLSTLVYQVGLLLGFQ"
BASE COUNT         2922 a         1127 c          988 g         2942 t
ORIGIN      
        1 attttttctt caaaaaataa taaaaagcaa gtaaaattac ttgcttttta tattaaattt
       61 tttcttaatt atatatacct aaacttatta tatatacctt taacttattt ttctttaaaa
      121 aaccaataag aatttaaccc agaaatttga accatagcat cttttgggac ttgttttatg
      181 gtattataaa tattcataaa atctccagac cctaaggtta aacttataaa accaaaagta
      241 aataaaattc ctgatataaa actcatatca ctaggtataa aaatccatat tataaggggt
      301 aaaaacccaa atactatgtt tggaaaaata cttaaaaata taaacctttt ctttgttatg
      361 gctgtattag atgtaactaa agccaacctt tgcttaatag aataccacat ttcaacctct
      421 gcatattttg gaaaaaatat agcatgtaga tattcatgcg gtaatatact aaatggtatt
      481 aagataattc caaaaatatt tagtacatca ctcactccaa aaaatccatt agtttttatt
      541 ctaagaaaaa taactataag aaaaaatatt acaacgggta ttaaaaatct tctagtttct
      601 ttagccagct cctcagctga ttccggttct tcaaacctta cagcgttttc aggcaattca
      661 cctattggaa gatcctctac atcgttaaac ttccctttcc aaattaactt caaaaaatta
      721 gccactaatc taataaaatc tttaaataag attgtaattt aattaaaatt aactacaact
      781 tatcctagta tttcttaaaa tacattaaat ataaaatatt ctaaatactt actttataat
      841 attagtgaca ttcacctccc ctatttgttt taaattatag ataaaattca aatataatac
      901 caaataacaa ctcactatac ttctcattaa taactcttta atgaaattct aagcacataa
      961 atataaactt tatatataaa tataatgttt acaaattaac ttccccttat tcccttatta
     1021 taccataaaa cctccatttt tcgtcatggt aaatcaaacc aattgaaaat tatttaattt
     1081 aatccatata ttaatatatc cttatataaa atcatatata taaatatata atttaaaatt
     1141 catacttatt aagggttctc attttaaaat ctccttctat atttaagaga accctacttc
     1201 ttaattaata tataggttta taattttcaa aggaggtttt tgataaagtt tataattaaa
     1261 tataaacttt acatatttaa ttatgagtat tgaaatttat gaaattgaaa gctttaaaga
     1321 tttgaatcta ttttggaata tgttccatga ttatataaac tatatttttg aaatgagtcc
     1381 actctctaaa gaaacatttg aatatttttt atcaaaagaa tataaagatt ctgttttaga
     1441 actttctgaa agagatttta acccactaaa aataatattt tttaagaaga aagacaaaat
     1501 actaggtttt gcaacttaca ttatatacat tgatgaaagt ggaaaatcta taatattaga
     1561 gtattgttta aacaaagatt ttagaaataa aggcttaggt aaaattgcat ataataaact
     1621 tcaagaaaaa atgataaaag aaggtgctaa aactatagaa cttaccccta ccaacaaaag
     1681 aaatgaactt ttttggaaaa acaatggctt ttctgatact agaatctttt tgcaagatgg
     1741 aagaaactta ttgataaaaa aactaaggta atattaagct ttttaaattt taaaattatt
     1801 tcatgtaaat tgtatagaaa tttaaaacta tgtctataat ccttaatgaa gatacaaaag
     1861 caatatattt aaagattaag tttaaatata ttacattttt attaataact aaatttaaaa
     1921 tagtgttgat tttatataaa catgtaaaac aacacaacta aaaatatagt taattaaatg
     1981 tgtagctatg ttaatgctct cctctaattc ttttttgcta attagctaca catttatttt
     2041 acaagctcaa ttatggattt aatccatttt acataattac ataaagttta taatagaata
     2101 aactctttaa agatcaataa actaaagaac cttatattta agtaatctta ttaatatagc
     2161 atctaagaat tacttaaaag aaatttacta aagtataaat ataataaact aatgaccact
     2221 ataataatat taccctaaaa tctcctttaa actaatctaa atattaatca aaataaaaag
     2281 cataaaccta cagacttagg tcttgctttt taaaatcact cttaataaga gaaagttaat
     2341 tctttctctt atttttttaa tgttataatt taaataaaac tcatttcttc ctttttgata
     2401 tgacttctta atattttatt ataaattttg tcttaaacaa cttttaagat cttcatctgg
     2461 agtacttatt ggcttaaggt caaaattatc aactaaatat tgaagtacat ttggtgataa
     2521 aaaagctggt aatgatggtc ctaaatatat tcctcttatc ccaagagcta atagtgccaa
     2581 taaatcagcc acggcttttt gttcatacca tgaaagtatt atgctaagag gcaatgaatt
     2641 tacattggta tcaaaagcat cagctaagct tgttgctatt ttaactgctg aatacaaatc
     2701 attacattgt cctatgtcta ataatcttgg taaccctgca acctctccaa actctaattt
     2761 attaaatcta tattttccac aagccaaagt taaaattata caatccttag gtactaattt
     2821 tgcaaagtct gtataataat ttcttccagg tcttgctcca tcacatcctc ctattacaaa
     2881 gaaatgtttt atatttccct cattaactgc agagactatt ttttctgcat tacttaaagt
     2941 cgctttgtgt ccaaaaccaa ctaaaatttc tttacattct tcatcctcat cataaccacc
     3001 aagttcaaga gccttgttta taatctctga aaaatcttta tatccatttt catcggcttt
     3061 tatatgtttt attccttccc aaccaactac actggtacta tatattctat ccttgtagct
     3121 ttccctaggt tgcattagac aatttgttgt cattaaaata catcctggaa tgtcatcaaa
     3181 ctccttctgt tggttttgcc atgcagatcc aaaattccca actaaatgct tatatttttt
     3241 taatccatca taaccatgag ctggtaacat ctcaccatgt gtatatatat ttattccttt
     3301 tccttcactt tgctctaaaa gcatttctaa atctcttaaa tcatgtcctg atactattat
     3361 aaaaggtcct ttttttaaag ttacatttac cttatgagga gtcggattct tatatttact
     3421 tgtattagca tcatctaata acttcattac tgcaatagct atatttccag ttttcatagt
     3481 taattctatt aaatcttctg agcttagttt atcattagta gtagcttcta aggctttaaa
     3541 ataaaaatta tctacttcct tatctataaa gttaataaac ctagcttgat gtccatatgc
     3601 tgctattccc ttcaatccat ataaaattgt tgatcttaaa gatcttatat cttgatctaa
     3661 gctatcatca tacattattc cagctttaat agcatctttt aacatctcat ctttactatc
     3721 acttaaatta tatatagcat gctctggata ttctcctttt agagcaatct ttctaagttt
     3781 ttcttttatt ttttgagatt ttttcagaag attgatatga acttctggat caaagtttac
     3841 attggttaag gttgtaaata gcccattttc tacaaacttt accacctctt gatcaatatt
     3901 tcctccttta tcaaggattt cttttgcata acatgatatt cccttcaatt ggtaaattaa
     3961 taaatcctga agatttgcta tttctggagt cttaccacac acccctcttt tagtacaccc
     4021 ctttcctcca actgtttgct cacattgata acaaaacatt aatgaatttt ccaacttaat
     4081 tcctccttta aactttattt attataatta acataaaatc aaattgttag atttaaaatt
     4141 aaatccttct ataaagatta ttgcttaata taattaaatc tataaattat aaatattatt
     4201 tcttattacg cgaaataatg ttaaacttaa ttcaaaattt taaatatact agtatattta
     4261 aagttattca ccaaaaccta gaaattacat ataaataatc atataggttt atacataaac
     4321 acctccttac ttttgtacag tatagtttta cctaaaaact tttttactat ccacatctcc
     4381 tttatttaaa ttccatacaa atttacttac acagcaaatt cactttaata aaaattatgt
     4441 ataaacttat attgatattt tagtaacctc attttaaaac tcatttttaa attaatttat
     4501 tttactaatt atgtgagaat atatttctca cttttttatt ttcatttcaa ataaaatcta
     4561 tttaaaaatt tcaagctaac aaattaccaa gtttcccctt tatttgttat ctaagaactt
     4621 aatcctctac ttattttatt tctttagaat agaaatcaaa gttaatacca gtatttacca
     4681 ggaaatctat ttctaaagga gttattttaa tctaatagat atattctact gctaaactca
     4741 attgaaaata attatcaaat tccttataat agcaattggt atcgattatt attgtttctt
     4801 agatgtgtaa agaggtgata taatgttaaa ggctatctta aaaataatga aaatttcaaa
     4861 tgaattctca tcctctacta ttgctaaaga acttaatata agtgaaacaa tggttgagat
     4921 ttataaagaa caactagaac aaaaaggata tataagaaaa atctctttat ctggctgctc
     4981 tcaagaacaa tgctcttcat gcggatgtgg ctgtgggagt tctaaaagtt taaattctat
     5041 ttcaggctgg gaaataactg agaaaggatt actagtttta caaaaataat tttaattaga
     5101 taaaattaag gtttttataa ttaggaggga tatacaaaat ggatacttta gaaaaaaagc
     5161 aagtaggtga aaagcttgtt gttaaaggaa ttcaaaaaga cagtattgta aaaagaagat
     5221 tattagatat gggaattacc cctggagtta ctttagaaat cacaggtaag gcccctcttg
     5281 gtgatcctat tgaagtgatt gtaagagggt ataaattaac tctaaggaaa aacgaggcta
     5341 aaagcatttt agtttagtat ttaatatctt aaaaggagga attttttatg atacctatgt
     5401 ctttgactaa agaaggcagt tctattcgga tagaaaaaat gcttattaat gattcactag
     5461 gaaaaagact tatggaaata ggaatttcaa agggaaatat tatagatgta attaaaaatg
     5521 atactacagg actaataatt ggtataagcg gatcacgtct tgctttagat aaaactttag
     5581 caaataaaat ctttgttaaa gaaatctaag ctatatattt ttgtttttaa ttgataatga
     5641 atattattat attttaaatg gtgtattggg atattttatg tattaataag aaacatactt
     5701 tgatataata atttattttt taattaatat ataaatggtt tatctaaaag accaaataaa
     5761 aatcttttag agttggtatt gggtatttta ctaaatttaa aattttatta agggggaaaa
     5821 ttaatgttaa ctattgcgtt agctggtaat cctaactgtg gtaagacaag cctttttaac
     5881 ttacttacta aatcaagaca acatatagga aactggcccg gtgttacagt ggaaaaaaaa
     5941 gaaggtactt taaaatttaa aggtgaaagc tataaagtaa ttgatttacc agggacttat
     6001 agcttaggag catactcaga agatgagatt gttgctagaa attatatatt aaaggacaaa
     6061 cctgatgttg ttataaatgt tgtggatgca actaacttag agagaaatct ttaccttact
     6121 actcagctca tagaaatggg cgcaaatgtg gttattgctc ttaatatgat tgaccaagct
     6181 gagtctttaa atatagaaat tgatacaaat aaactttcta aaagattagg tgttcctatt
     6241 ataaaaacat cagcttttaa aaatagagga atagaagaat taatagaaac ttctatacat
     6301 tcaaaaaaga atgaaaaact tatcaatata aattatggtg aagatattga aaatgaaata
     6361 aaaaaccttt cttatttgct agaagcatat aaaaataaat tggaattccc tgttaattgg
     6421 acagctttaa aacttttaga aaatgatgaa tatataaaag ataaagtaaa acaattaaat
     6481 tctccaagta tatttactaa gcttgaagaa agtaataaga ccatagaaaa aaatataggc
     6541 tttgaagctg atatgtctat tgttgataaa agatattctt ttatatcatc aattactgaa
     6601 gatgtaataa agaaacctag taaaaagcaa gtaacaacta ctgaaaaaat agataaaata
     6661 gttacaaata aatatttagg aatccctatt ttcgcactta ttatgtattg tttatatgaa
     6721 ttaactttta ttattggtgc tggaattcaa gaatggtttg gagatcttat agctaaagca
     6781 ggtgtaattg tatctgactt tattgctaat atgggtgctc ctgaattact agtaggattt
     6841 ataaatgatg gactatttgg tggtgtaggt gctgttcttt ctttcttacc attaattatg
     6901 gttatgtact tcttattagg acttttagaa gacagtggat acatggcaag agctgcttac
     6961 gttatggata gacttatgag aggtttagga ttacatggaa aaaccttcgt ctctatgata
     7021 gtttccgttg gatgtaatgt tcctggaata atgtctacta gaaccttaga aaacaaaaag
     7081 gatagaatga tagctatact tattaatcca tttatatcct gtggtgctag aatgcctata
     7141 tatgctgtat ttgttgaagc attcttccct actcatcaag gtttagtctt atttagttta
     7201 tatgtgctag gtattatagt tgcattaata agtggaaaaa tatttagcaa aactttattt
     7261 aaaggtgaat cttcatattt tgtaatggag cttcctgctt atagaattcc atctataaag
     7321 aatgtattct tattaatgtg ggaaaaagca ggtgctttct tcaagaaagc tggaatgatt
     7381 atattcccaa tgatgatagt tctttgggct ttatctgtat taccactagg agttgaacca
     7441 aatagtgaac actctatttt aggtatgatt ggtagctttg ttgctccatt atttgtacta
     7501 gctggatatg gaacatggca agctggtgta tcattaataa ctggtatatt agctaaagag
     7561 tcagtagttg caacaatggg tatggtttat gctggagttg aagaagggga agccttaata
     7621 aatgtaattc aacaggtatt tactccactt tcagcagttt cattcttagt tatgactctt
     7681 ttatatactc cttgtttagc agccttaggg gtaataaaaa gagaaactaa ttctatgaaa
     7741 tggactatat tctctgctgt atatacattt gtaattgcct tagttttatc aacacttgta
     7801 tatcaagttg gattattgct aggttttcaa taaattaaat atgccttcta attataatta
     7861 ttttagttat aattattata agaaccaaga gatctccttc caatagtctc ttggttcttt
     7921 caatttaaaa tattatatat aaacttagaa aatatccttt tttcccccct ctatttgct
//
LOCUS       DAEVGI010000114         7815 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEVGI010000114 DAEVGI010000000
VERSION     DAEVGI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.114"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            81..197
                     /locus_tag="K8M77_001934"
                     /pseudo
     CDS             81..197
                     /locus_tag="K8M77_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
     gene            387..617
                     /gene="secG"
                     /locus_tag="K8M77_001935"
     CDS             387..617
                     /gene="secG"
                     /locus_tag="K8M77_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBI7086415.1"
                     /translation="MNTILVVVQTILAIVILVSIMMQPSKADALSGLIQGSQDSFFSR
                     NKGRTREARLIKVTIFASVLFAINTLAINLVK"
     gene            906..3161
                     /gene="rnr"
                     /locus_tag="K8M77_001936"
     CDS             906..3161
                     /gene="rnr"
                     /locus_tag="K8M77_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBI7086416.1"
                     /translation="MKIKDIILGFMKEEAYRPMTMLELSKIFNINKAEYKQFKKVFKA
                     LEAEGLIARDEEDRFGLIDKVGLIPGKIQIHQNGFGFLIPDEEGVTDVFIPKNALNEA
                     MNGDRVLGKVVKESQEGKRREGEIVRITERVNTKIIGIYEDNKSFGFVLPQDQRIQND
                     IFISKKDRNGAKKGNVVVVEITKWPDGRRKNPEGVVVEILGNRGDLGLDIITVIKKYA
                     LPEEFPPQVLAFAEEISEEIPESEIKRRTDLRDVKMVTIDGEDAKDLDDAVSIERLPN
                     GNYKLGVHIADVTHYVKENNPLDKEAFKRATSVYLVDRVIPMLPKKLSNGICSLNPQV
                     DRLALTCFMEIDHKGNVVNYSIHESVIKTNERMTYTDVTKILRDNDKELIERYKDLVD
                     DFKAMEELCKILNKRRMGRGAIDFEFAESKIILDEKGKPIDIKPYERAIANRIIEEFM
                     LVCNETIAEYMNKAEIPFVYRIHEEPDNEKLQRFKNFAYSLGYLFNFGELIEPRDLQK
                     VVEEVKGKNEEMVINTLLLRSMMQARYSPDCAGHFGLAAEYYCHFTSPIRRYPDLQIH
                     RIIKEYINKKIDPKRAKRLLSIVDSASKQSSEMERIAQEAERETNDLKKAEYMKDRIG
                     EIFDGIISSVTGFGAFVELPNTVEGLVHVTSFRDDYYIYDEENLQLIGERTKKVYKLG
                     EKVKVLCSRVEMDTREVYFEIVEDKYTIEEKEAETEERENLLDEVKKELEAEGKVEII
                     EPREETAEEEL"
     gene            3517..3987
                     /gene="smpB"
                     /locus_tag="K8M77_001937"
     CDS             3517..3987
                     /gene="smpB"
                     /locus_tag="K8M77_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBI7086417.1"
                     /translation="MAKRKDNKTLAENRKARHDYFVDEAMEAGIALVGTEVKSIRNGR
                     VNLKDCYADINNGEIFINNMHISPYEQGNIFNVDPLRKRKLLLHKSEIQRLIGIVQQQ
                     GVALIPLSLYLKNGRVKVNLGVCRGKKNYDKRDTMLEKAHKREMDRQLKERNRY"
     gene            4471..5079
                     /locus_tag="K8M77_001938"
     CDS             4471..5079
                     /locus_tag="K8M77_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086418.1"
                     /translation="MRVLKGVILAMIPAVLFMGCGEFSKNDVAKVSFEPLSEKEEQIL
                     NLTDNRVFFYKVKNISKGKGYKIDLSYEVYKEGKEVKNEPILTSVSETYEKGKENITL
                     GINFKDDNRINCLLGGDGVYSRHNYKSEENIKDYLSANFAGDYDLELEKGKRVCLYYG
                     TSGNGISSNVIGMYMDSEEIKEAINKSKDCIFLSISVEDFSE"
     gene            complement(5308..6297)
                     /locus_tag="K8M77_001939"
     CDS             complement(5308..6297)
                     /locus_tag="K8M77_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI7086419.1"
                     /translation="MTSFQSLIIMLICILSIIIILYLIESDNFLLEKQISKYISLGKF
                     KHALYLLDKYSEKLDPLFFHNNKILILSKQKDFDAAYEISIEAIKKLKEPNYELYNNI
                     AFVYYNLKLYNRAIDFSERALKLNPIDTFALNNKGFSYIEIGEYSKAEECFDKALEFN
                     PYFKNALFGKAYCAFEKSDYLLATKYLQDFVSIEKNNASAYKKLGECYFILNDLKNSS
                     KMYEKSLEIDQENDASYCEYANVLLCLGHYDKALNNLNKALAISPYNFMAFYYKARAY
                     SLKKDFDNAFYYLERAFNYCDSLKEIALKDEMLTNLKFFSKFSELLEIHNIKN"
     gene            6470..7048
                     /locus_tag="K8M77_001940"
     CDS             6470..7048
                     /locus_tag="K8M77_001940"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI7086420.1"
                     /translation="MELLKDKIKKEGRVTEDNILMVDSFLNHQMDVEFFNEVGKEFKD
                     RFKNDQIDKILTIESTGIGLGIITAQYFDNVPVVFGKKIERLPRNKREEVFNSEVYSF
                     TKKTIYNVVVDKKFIKPGEKILIVDDFLANACAVFGLIDVVKQAGAEVVGVGIVIEKG
                     FQSGRAHLEDKGIRVESLVTIDKIEDGEVYFK"
     gene            7374..7734
                     /gene="ssrA"
                     /locus_tag="K8M77_001941"
     tmRNA           7374..7734
                     /gene="ssrA"
                     /locus_tag="K8M77_001941"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         3098 a          776 c         1422 g         2519 t
ORIGIN      
        1 aaaaattcct ttaagaaaaa tggattatta gaaggatttt aaaatgaatt taagaactaa
       61 tacaaaacta ataataaatg ctttattatt taactgtttt agtgccttca gttgcattaa
      121 atactgttat aggattagta ttatatagag ttatagaaaa ttctctaaga agagcctctt
      181 atagtataag aggatagaaa attaaatgag ttatataatc atagaaagag gaaaaagtat
      241 ataaaagaaa aataggtaaa ctatataata tgtattgttt tataaaagtg tgatgaacaa
      301 ggatataaat attgtaaatt tttcattggt gtgatatgat aatttcatgg aattatgcaa
      361 ctatacaaag cttaggaggt tttcttatga acacaatttt agtagtggtc caaactattt
      421 tagctatagt aattttggtt tcaataatga tgcaaccaag taaagcagat gccttaagtg
      481 gattaatcca aggaagtcaa gatagcttct tctcaagaaa taaaggtaga acaagagaag
      541 ctagacttat aaaagttact atctttgcat ctgtattatt cgctataaat acattagcta
      601 taaatttagt taaatagtta ttttttggcc acccatataa ttagggtggc ttttaaatta
      661 tattggattg attaaaattt catatttaac attttacaag aatggtttta gattaatatt
      721 ggattgaatt tccttaatca aagagaggat ttaagcatat catattagta ggatgataaa
      781 tcaatgtttt ctagcagtat cttagataat taggtgagtt aatccattag aatttaagag
      841 aaacaggctt tgagctaaaa tataaaagtt gcatttaaat ctatatatag aacggaggaa
      901 aaagcatgaa aataaaagat ataattttag ggtttatgaa agaagaagca tacagaccaa
      961 tgacaatgtt agaattatca aagatattta acataaataa agctgaatat aaacaattta
     1021 aaaaagtctt taaggcctta gaagcagagg gtttaatagc tagagatgag gaagacagat
     1081 ttggcttaat agataaagtg gggttaatac ctggaaaaat tcaaatacat caaaatggat
     1141 ttggtttctt aataccagat gaagaaggtg taactgatgt atttatacct aagaatgctt
     1201 taaatgaggc tatgaacgga gatagagtat taggtaaggt tgtaaaagaa agccaagaag
     1261 gaaaaagacg tgaaggtgaa atagtaagaa taactgagag agttaataca aagataatag
     1321 gaatatatga agataataag agttttggat ttgtacttcc acaggatcaa agaattcaaa
     1381 atgatatatt catatctaag aaggatagaa atggagctaa aaaaggtaat gtagttgttg
     1441 ttgaaataac taagtggcca gatggtagaa gaaagaatcc agaaggagta gtagtagaaa
     1501 tactaggaaa cagaggagat ttaggcctag atattataac tgtaattaag aaatatgctc
     1561 ttccagaaga attccctcca caagttttag cctttgcaga agaaatttca gaggaaatcc
     1621 cagagtcaga aattaagaga agaacagact taagagatgt aaaaatggtt actatagatg
     1681 gtgaagatgc taaggattta gatgatgctg tatctataga aagacttcca aatggaaact
     1741 ataaattagg agttcacatt gcagatgtaa ctcactatgt aaaagaaaat aatcctttag
     1801 ataaagaggc ctttaaaaga gctacttctg tttacttagt tgatagagtt attcctatgc
     1861 ttcctaaaaa gttatcaaat ggaatatgtt cattaaatcc acaggtagac agacttgctt
     1921 taacttgttt tatggaaata gatcataaag gaaatgtggt taactattca atacatgaaa
     1981 gtgtaattaa aactaatgag agaatgacat acactgatgt aactaagatt ttaagagata
     2041 atgacaaaga gttaattgaa agatataagg atttagtaga tgactttaag gcaatggaag
     2101 agttatgcaa aatcttaaat aagagaagaa tggggagagg agctattgac tttgaatttg
     2161 cagagagcaa aataatctta gatgaaaaag gaaagccaat agatataaaa ccatatgaaa
     2221 gagctatagc aaacagaatc atagaagaat ttatgctagt atgtaatgaa actatagcag
     2281 aatatatgaa taaggctgaa atcccttttg tatatagaat acatgaggag ccagataatg
     2341 aaaaacttca aagatttaaa aattttgcat atagcttagg atatttattt aactttggag
     2401 aacttattga gcctagagat ttacaaaagg ttgttgaaga agttaagggc aaaaatgaag
     2461 agatggtaat aaatacattg cttttaagaa gtatgatgca agctagatat tcaccagact
     2521 gtgcaggtca ctttggatta gcagctgaat actactgtca ctttacttca ccaattagaa
     2581 gatatccaga cttacaaatt catagaataa taaaagaata tataaataag aagattgatc
     2641 ctaagagagc taagagactt ttaagtattg ttgactctgc atctaaacaa tcttcagaaa
     2701 tggaaagaat agctcaagaa gctgaaagag aaacaaatga tcttaagaag gctgaatata
     2761 tgaaagatag aattggagaa atatttgatg gtattatttc ttcagttact ggatttggag
     2821 cctttgttga acttccaaat actgtagaag gtcttgttca tgtaacaagc tttagagatg
     2881 attattacat ctatgatgag gaaaaccttc aattaatagg tgaaagaact aagaaggttt
     2941 ataaacttgg agaaaaggtt aaggttcttt gctcaagagt agaaatggat actagagaag
     3001 tttattttga aatagttgag gataagtaca ctattgagga aaaggaagct gaaactgaag
     3061 agagagagaa tttacttgat gaagtaaaaa aagagcttga agcagaggga aaggttgaaa
     3121 taatagagcc aagagaagaa actgctgagg aagagcttta ataataggaa gataaaaaac
     3181 ttatagatag atttcgagaa ttactacatt tcattaagaa tttatagtag tcactccaag
     3241 taagttacta aaacctgaaa acttgctgcg cttcggacat tcaggtttat taacgtaact
     3301 tactttacgt gactacttaa attctaaaat tcatgtagat attctctata ttctatctat
     3361 aagttttatt aatatcttaa tataattaag tgtagttttg tgaatttaca tttaaatagt
     3421 aaatatgtta aaataataag caaagaaaag aaataagagg cgttttgctt tgggtaagta
     3481 aaattgcttt taatataacc atagtaggtg agaagtatgg caaagagaaa agacaataag
     3541 acattagcag agaataggaa agctagacat gattactttg ttgatgaggc tatggaagca
     3601 ggtatagctt tagttggaac agaggttaag tctattagaa acggaagagt taatttaaag
     3661 gattgttatg cagatataaa taatggagag atatttataa ataatatgca tataagtcct
     3721 tatgagcaag gaaacatatt taatgttgat cctttaagaa aaagaaaatt acttcttcat
     3781 aaaagtgaaa ttcaaagatt aataggaata gttcaacaac aaggggttgc tttaatacca
     3841 ctttctttat atttaaagaa tggaagagtt aaggttaact taggggtttg tagaggtaag
     3901 aaaaactacg acaagagaga tacaatgctt gaaaaggctc ataagagaga gatggataga
     3961 caacttaagg aaagaaatag atattaaatt cttttctaga aggtgcttag aagaaaaagt
     4021 tattatttta atttaaaaac tttatctttt aagcaccttt tttatattta agtcaaggca
     4081 cttttgctta ttaattaatt tataaagttt tagattaaat taggttacta tatgttttta
     4141 ttaacttata taaagaagga aatttataag gggaaggaga aaagatttaa gggggttaaa
     4201 gtttataagg gaattaattt atgaaactac aagttaatag gaaactacaa cttaaaatga
     4261 ggtagtattt atggggataa attaaaaata gttagtgcct taggaaagtt ttgaaaatta
     4321 aaaatataca agactttaag taaaaataaa tttttaaaat actagtttag ggttttatca
     4381 tataatataa aaaaatagtt acaattaatg taattaatat aaatatggat ttataaatta
     4441 taatataaaa attaatatag gggtgtttta atgagagttt taaaaggggt aattttagct
     4501 atgattccag cagtcttgtt tatgggatgt ggagagtttt ctaaaaatga tgttgcaaag
     4561 gtaagttttg agccgctttc agaaaaagag gagcagattt taaatttgac tgacaataga
     4621 gtttttttct ataaggtgaa aaatatttct aaaggtaagg ggtataagat tgatttaagt
     4681 tatgaggttt ataaagaggg aaaagaagtt aaaaatgaac ctattttaac aagtgtaagt
     4741 gaaacctatg aaaaggggaa agagaatata accttaggaa taaactttaa agatgataat
     4801 aggataaatt gtcttttagg aggagatgga gtttatagtc gtcataatta caagtctgag
     4861 gaaaatataa aggactattt aagtgctaat tttgctggtg attatgattt agaacttgaa
     4921 aaaggaaaga gagtttgtct ttattatgga acttcaggaa atggaattag tagtaatgtt
     4981 ataggaatgt acatggattc agaggaaata aaagaagcta ttaataaaag caaggactgt
     5041 attttcttaa gtataagtgt agaggatttt agtgagtagt ttataaattt attttagaga
     5101 ttaaaataaa tttaaaagag tacatgttcc ttataaaagt tagtagttta taaatagaat
     5161 ttagagcctt aataaagtta gtatagaaac taagtattat tagggctcat attttaaatt
     5221 tcttatataa tatttttctg tagaaattta aaaggaagta taattaaaaa attaattaga
     5281 ataattttta gcttttcact ttaaaagtta gttttttata ttatgaattt ctaataactc
     5341 actaaacttt gagaaaaact ttaagtttgt aagcatttca tccttaagag ctatttcttt
     5401 taaggagtca caatagttaa aggccctttc taaataataa aatgcattat caaaatcttt
     5461 ctttaaggag taagctcttg ctttataata aaaggccata aagttatagg gtgatatggc
     5521 aagggcctta tttaaattgt ttagtgcctt atcatagtga cctaagcaaa gaagtacatt
     5581 tgcatattca caataagaag catcattttc ttggtctatt tctaaggatt tctcatacat
     5641 tttagaactg ttttttaagt catttaaaat aaagtaacat tctcctaatt ttttataggc
     5701 agaggcatta tttttttcaa ttgaaacaaa atcttgaaga tatttagtag caagaagata
     5761 atcactcttt tcaaaagcgc aatatgcctt tccaaataag gcatttttaa aataaggatt
     5821 aaattctaat gctttatcaa aacattcctc agctttagag tattcgccta tttcaatata
     5881 agagaaacct ttattgttta aagcaaaggt atctatagga tttaatttta aggctctttc
     5941 tgaaaaatct atggctctat tataaagttt tagattgtaa tatacaaatg caatattatt
     6001 gtaaagttca tagttaggtt ctttaagctt ttttattgcc tctatggaaa tttcataggc
     6061 tgcatcaaaa tctttttgct ttgatagtat taaaatttta ttattatgga aaaatagagg
     6121 atctaatttt tctgaatact tgtctaaaag ataaagagca tgtttaaatt ttcctagaga
     6181 tatatattta cttatttgtt tttctagcag aaaattatct gactcgatta aatataaaat
     6241 tataattatg gataaaatac atataagcat tattattaat gattgaaatg aagtcatatt
     6301 tatctctcct aaatattctt ctactattat tatataaaga attactaaaa aggattacat
     6361 gaataataag aatactgtat aataaaatta gatagcaata ggttttccta ttatgatata
     6421 atataggaag ttaatactat aaattaatag taagaaagga tgtaaccaaa tggaactatt
     6481 aaaagataag ataaaaaaag aaggtagagt tacagaagat aatattttaa tggttgattc
     6541 ttttttaaat caccaaatgg atgtagaatt ttttaacgaa gtaggaaaag aatttaaaga
     6601 tagatttaaa aatgatcaaa tagacaaaat ccttacaata gaatcaacag gaataggatt
     6661 aggaataata actgctcaat attttgataa tgtaccagtt gtttttggta agaaaataga
     6721 aagattacca agaaacaaga gagaagaggt atttaattca gaagtttatt cattcactaa
     6781 aaaaacaata tacaatgttg ttgttgataa gaaattcata aaaccaggag aaaaaatcct
     6841 tatagttgat gatttcttag caaatgcctg tgcagttttt ggattaatag atgttgttaa
     6901 acaagctgga gcagaagttg ttggagtagg aatagttata gaaaaaggat tccaaagcgg
     6961 aagagctcac ttagaagaca aaggaataag agttgaatct ctagttacta tagataagat
     7021 agaagatgga gaagtttact ttaaataatt tttttagatt atttttatga gctaaatagg
     7081 gttaaaaagt acattttaag cctaaagaga gcaaaagata agaaatttta atttaggtaa
     7141 aattaaccat tgatatttta attagaatta ggtattatat aaattgtctg ttttagaatg
     7201 ttaaaagtta attaattagt caaggaattt atctcttaca aacaaaaata tatgtccctt
     7261 gactaaatta attaattaat atataataag aacataagtt tgattaaaac tttagaattt
     7321 aaaaattgaa tatggaattt ttagcgttta gaccatatta ttatctcata catgggggcg
     7381 ttttggcttc gacgggggta agatgggttt gataagcgag tcgagggaag catggtgcct
     7441 cgataataaa gtatgcatta aagataaacg cagaagataa ttttgcatta gcagcttaat
     7501 ttagcgctgc tcatccttcc tcaattgccc acggttgaga gtaagggtgt catttaaaag
     7561 tggggaaccg agcctagcaa agctttgagc taggaacgga atttatgaag cttaccaaag
     7621 agaaagtttg tctgtggacg ttctctgagg gaattttaaa acacaagact acactcgtag
     7681 aaagtcttac tggtctgctt tcggacacgg gttcgacacc cgtcgcctcc accataaaaa
     7741 accacgatag aaatatcgtg gttttttata tgctaaatga ataaatatat aaaggcaata
     7801 ttaaatatat tttga
//
LOCUS       DAEVGI010000115         7622 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEVGI010000115 DAEVGI010000000
VERSION     DAEVGI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.115"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..924)
                     /locus_tag="K8M77_001942"
     CDS             complement(<1..924)
                     /locus_tag="K8M77_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7086421.1"
                     /translation="MFLKSLEIRGFKSFADKTELNFKKGITAIVGPNGSGKSNVSDSV
                     RWVLGEQSAKTLRGAKMEDVIFTGTEYRKPIGYAQVSLTLDNSSGELPLDYLDVKVTR
                     KLFRSGESEYLINNSPCRLKDVVNLFMDTGIGKEGYSLIGQGKIDSILSGKPEDRRAI
                     LEEAAGIVKFKSRKGEAEKKLKNTEENLVRINDIIFTYEERLGPLEEEKNKALRYLEL
                     SNELKHKEISSLMSELHNIDGVILKQKEKIKDLIAFNDKQKEKYKEEREKMFALKEEL
                     DSLEGSHSKKQEEYFVKKEEINKSLGKVNLLN"
     gene            complement(941..1036)
                     /locus_tag="K8M77_001943"
     CDS             complement(941..1036)
                     /locus_tag="K8M77_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017750641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBI7086422.1"
                     /translation="MRKTTRDRVIKITIIFVVICFLATLLPIMFM"
     gene            complement(1129..2202)
                     /locus_tag="K8M77_001944"
     CDS             complement(1129..2202)
                     /locus_tag="K8M77_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI7086423.1"
                     /translation="MGKRHYIIPIFVPHEGCPHDCVFCNQGKITGENKEIILGPKYKQ
                     ENKVNSNFVRKTIEEYIETIGEGDRILEVSFFGGTFTAIDINKQRELLAVAKEYKDKK
                     IIDYIRLSTRPDYIDEFILDHLKSYKVDIIELGVQSLDKEVLHKSGRGHGYDEVLKAS
                     KLIKEYGFTLGHQIMVGLPEDTFEKDIETTRESIKMKPDICRIYPALTVKNTPMEDMY
                     LEGTYKPYTLEEAVYISAKLYKMYKENNIQVIRIGLQPTDNIALGKDIVDGPFHPAFR
                     ELVESSIINENIYNILKDKSGEVTIRISNKSVSKLYADKKRYFNELKDKAQNCNLKVK
                     VDNSMEVDKINIEVESKVYKIDL"
     gene            complement(2181..2906)
                     /locus_tag="K8M77_001945"
     CDS             complement(2181..2906)
                     /locus_tag="K8M77_001945"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBI7086424.1"
                     /translation="MNFKVKDINLIEVEKLIGIEFENKGVLITALTHSSYANQFKDVK
                     YNERLEFLGDSVLQLCVTKYLFNNYKDKSEGELTKIRALVVCENSLHKVSKNLSLGKY
                     IRMSKGEELTGGRERTSIQADALEAVIAAVYLDKGIEVANDFILRNFKDVIDKAINEE
                     IILDFKTRLQEVLQKNGEVNIVYNLVKHEGPPHRRKFFTDLLINNEVMGQGVGFSKKE
                     SEQNAAKAALQRLGEIKWEKDTI"
     gene            complement(2993..3223)
                     /gene="acpP"
                     /locus_tag="K8M77_001946"
     CDS             complement(2993..3223)
                     /gene="acpP"
                     /locus_tag="K8M77_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI7086425.1"
                     /translation="MFDKLKEIIADKLSVNEDEITMESTFIDDLGADSLDIVELIMAL
                     EEELEMEIPDEDAEGFKTVGDVVEYITEHTEK"
     gene            complement(3300..4319)
                     /gene="plsX"
                     /locus_tag="K8M77_001947"
     CDS             complement(3300..4319)
                     /gene="plsX"
                     /locus_tag="K8M77_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBI7086426.1"
                     /translation="MRIAVDGMGGDHSPSAVVEGCVQALEEFKDIEIYITGPEDLLKE
                     AFSKFKYDKERVIFIDAKEVISTNEHPAMAVKKKKDSSLVKALRLVKDNQCEAVISAG
                     STGAFLTGCTLIVGRIKGVERPALAPVMPGKNGPFMIIDAGANVDSKPSYLVQFAKMG
                     EVYFKSVMDVNNPKVGLVNIGEEEEKGNDLTKATYKLLKEEKDINFIGNVEPREVSTG
                     DVDVLVCDGFVGNTVLKMYEGVASTILSMIKSEVKSSFLAKLGVPFLAPALMNLKKKM
                     DYKEYGGAPFLGVKGICVKAHGSSDAKAFKNAIRQARKFHENDLIGKLSEEITKKSFD
                     NQKKF"
     gene            complement(4376..4558)
                     /gene="rpmF"
                     /locus_tag="K8M77_001948"
     CDS             complement(4376..4558)
                     /gene="rpmF"
                     /locus_tag="K8M77_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBI7086427.1"
                     /translation="MGNPARKTFRAKRDSRRAQTFKASLPGIVECPQCHEMKMAHRVC
                     KNCGHYKGKEVVSVEE"
     gene            complement(4574..5068)
                     /locus_tag="K8M77_001949"
     CDS             complement(4574..5068)
                     /locus_tag="K8M77_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBI7086428.1"
                     /translation="MKLIFSEICSKRERRKSLDFILDLKSFEFEGENMKVIEPLNFTG
                     EVISNESFIELVGNITGELQMKCSRCLTNFSYEISIDMDEKFTNNSNQEDDSIAYVEG
                     DELNVAEAVVENVISTLPIKRLCSIDCNGLCQKCGIDLNKETCQCDNEEIDLRMAKLM
                     DLFK"
     gene            complement(5188..6384)
                     /locus_tag="K8M77_001950"
     CDS             complement(5188..6384)
                     /locus_tag="K8M77_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBI7086429.1"
                     /translation="MNVLVINCGSSSLKYQLIDMDTENALATGLVERIGLEGANLTQK
                     SEGKDKYEIIEPMKDHQDAIRLVLGALIDEKHGVIKSLDEINAIGHRVVHGGEKYAES
                     ALVTEEVMKDLEECAKLAPLHNPANIIGINACKALMPNVPMVVVFDTAFHQTMPEKAF
                     VYALPYELYEKEHIRKYGFHGTSHKYVSAKIAEAMGKNIEDLKIITCHLGNGASIAAI
                     KNGKCVDTTMGFTPLEGLVMGTRCGNIDPAVVTYLIDELGYTSQEVNTLMNKKSGIFG
                     VSGVSSDFRDVEAAADKGSKEAQIALDLFRNSVKKYIGAYIAEMNGCDVIVFTAGVGE
                     NSIIERGAICRDLEFLGIELDEERNNIRAKVAEISKEGSKIKLFVVPTNEELMIAQDT
                     LSIVSK"
     gene            complement(6474..7475)
                     /gene="pta"
                     /locus_tag="K8M77_001951"
     CDS             complement(6474..7475)
                     /gene="pta"
                     /locus_tag="K8M77_001951"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI7086430.1"
                     /translation="MELMKQIWESAKNNRKKIVLPEGDEERTLVASQRIKEEGLADVY
                     LVGSEQVIREKAEALGVNLEGVNIVDPETSDKLDTYINEFYELRKAKGMTVEKAGKIV
                     RDPLYFGTMMVKMGDADGMVSGAVHTTGDLLRPGLQIIKTAPGVSVVSSFFIMMVPGS
                     QYGEGGMLLFSDCAVNPNPNADQLAAIAIATADTAKNLCKMDPKVAMLSFSTMGSADH
                     DLVTKVRVATEKAKELRPDLDIDGELQLDAAIVEKVASQKAPNSKVAGNANVLVFPDL
                     QAGNIGYKLVQRFANAEAIGPVCQGFAKPINDLSRGCSSEDIVNVVAITAVQAQATK"
BASE COUNT         2267 a         1521 c          815 g         3019 t
ORIGIN      
        1 atttaaaaga tttacttttc ctaagctttt atttatttct tcctttttaa caaaatactc
       61 ttcttgcttt ttagaatgac taccttctaa agagtctaac tcttccttta aagcaaacat
      121 tttctctctt tcttctttat acttttcttt ttgcttatca ttaaaagcta ttaaatcttt
      181 tatcttttct ttttgcttta atataacccc atcaatatta tgtaattcac tcataagaga
      241 tgaaatttct ttgtgcttta attcatttga aagctctaaa tatcttaaag ctttattttt
      301 ttcctcttct aaaggaccta atctttcttc ataagtaaaa attatatcat ttattctaac
      361 taagttttct tccgtattct ttaacttttt ctcagcttca ccctttctag atttaaactt
      421 tacaatacct gcagcttcct ctagaatagc tcttctatct tccggcttac cacttagaat
      481 actgtctatt ttaccttgac caataagcga atacccttct tttcctattc ctgtatccat
      541 aaataggttt acaacatctt ttaatctaca aggagaatta tttattaaat attcgctttc
      601 tccagaacgg aataattttc tagtaacttt tacatctaaa taatctaaag ggagttcccc
      661 tgaagaattg tctaaagtta aagaaacctg tgcatatcct ataggctttc tatattctgt
      721 accagtaaag attacatctt ccatcttagc tcctcttaag gttttagcac tttgctcccc
      781 taaaacccat cttacgctat ctgatacatt actttttcca cttccattag gcccaactat
      841 agccgttatt ccttttttaa aatttaattc tgttttatca gcaaatgatt taaatcccct
      901 tatttcaaga gattttaaaa acatatattc actcctatct ttacataaac ataattggta
      961 ataaagttgc taaaaagcat attacaacaa aaataatagt aatttttata accctgtctc
     1021 tcgtagtttt tctcactgta cttcactcct catggtataa gtttatatta tgccaattaa
     1081 aatatcaata aaaaaagcct cagagataaa ctccaaggca tataataact ataaatctat
     1141 tttatatact ttcgattcta cttctatatt tattttatct acttccatag aattatctac
     1201 tttaaccttc aaattacaat tttgtgcctt gtctttaagt tcattgaagt atctcttttt
     1261 atcagcataa agcttagaaa ctgatttatt gctaattctt atagttacct ctccactttt
     1321 atcctttaag atattatata tattttcatt tataatacta ctctctacta attccctaaa
     1381 agcaggatgg aaaggcccat ctacaatatc cttacctaaa gctatattat ctgtcggctg
     1441 caaaccaatt cttataacct gtatattatt ttctttatac atcttataaa gtttagcact
     1501 tatatacaca gcctcttcta gagtatatgg tttataagtt ccctctaagt acatatcctc
     1561 cataggagtg tttttcactg taagagcagg atatattcta catatatcag gtttcatctt
     1621 tatagactct cttgttgttt ctatatcctt ctcaaaagtg tcctcaggaa gtcctaccat
     1681 tatttgatga cctaaagtaa agccatattc tttaattaac ttagaagctt ttaaaacctc
     1741 atcataacca tgacctctac ctgatttatg caacacttcc ttatctaaag actgaactcc
     1801 aagttctatt atatcaactt tataactttt taaatgatct aaaataaact catcaatata
     1861 atctggtcta gtagacaatc ttatatagtc tataattttt ttatccttat attcttttgc
     1921 aacagctaat agttctcttt gcttatttat atcaatagca gtaaaagttc ctccaaagaa
     1981 agaaacctct aatattctat ctccttcacc aattgtttct atatattctt caattgtctt
     2041 tcttacaaaa ttactattaa ccttattttc ttgcttgtac tttggtccta atataatctc
     2101 tttattttct ccagttattt tcccttgatt acaaaacacg caatcatgtg gacatccttc
     2161 atgaggaaca aaaattggga ttatatagtg tctttttccc atttaatttc acctaacctt
     2221 tgcagtgctg ctttagctgc attttgttct gattcttttt tactaaatcc aacaccttga
     2281 cccataacct cattattaat taaaagatct gtaaagaatt ttcttctgtg aggtggtcct
     2341 tcatgcttaa ctaaattata aacaatatta acttccccat ttttttgaag aacttcttgt
     2401 aatctagttt taaagtctaa gattatttct tcatttatag ccttatctat aacatcttta
     2461 aagtttctta aaataaagtc atttgcaact tcaattcctt tatctaaata aactgcagca
     2521 attactgcct ctaatgcatc ggcttgtatt gaagttcttt ctcttcctcc agttaactcc
     2581 tcacctttac tcattcttat atacttacca agtgatagat tttttgaaac tttatgtaaa
     2641 gaattttcac aaactactaa agctcttatt tttgttaatt caccttcaga tttatctttg
     2701 taattattaa ataaatattt tgtaacacaa agctgtaaaa ctgaatctcc taaaaattct
     2761 aatctttcat tgtattttac atctttaaat tgatttgcat atgaactatg cgttaatgct
     2821 gttataagca ctcctttatt ttcaaactca attcctataa gtttttcaac ttctattaaa
     2881 tttatatctt tcaccttaaa attcatctcc ttaaggttta ttcttcaagt tgtttgtgaa
     2941 acaacttcaa gaataaactt aaattaaaaa tcccgcttat tgcgggactt tattacttct
     3001 ctgtatgttc tgtgatgtat tctactacat ctcctactgt tttgaagccc tcagcatctt
     3061 catctgggat ttccatctct aactcttctt caagagccat tattaattca actatatcaa
     3121 gagaatccgc acctaagtca tctataaatg ttgattccat agttatttca tcttcgttta
     3181 cgcttaattt atcagcaata atctctttta atttatcaaa cattcaattc acctcctaag
     3241 ttaatctaat aaaatattat tatatataaa atgcatagtc aatacgcatt acgtaatttt
     3301 taaaattttt tttgattatc aaagcttttt ttagtgattt cttcacttaa tttcccaatt
     3361 aggtcatttt catggaattt tctcgcctgt cttattgcat ttttaaaagc ttttgcatca
     3421 gaactaccat gtgcttttac acatattccc tttacaccta aaaatggtgc tccaccatat
     3481 tctttataat ccattttttt cttaaggttc attaaagctg gagctaagaa tggtactcct
     3541 aatttagcta aaaatgatga tttaacttct gatttgatca tacttaatat tgtagaagct
     3601 acaccttcat acatctttaa aacagtattt ccaacaaatc catcacaaac taagacatct
     3661 acatctcctg ttgaaacttc tctcggttca acatttccaa taaagttaat atctttttct
     3721 tcttttagaa gcttataagt agcctttgta aggtcattac ccttttcttc ttcttctcca
     3781 atattaacta atcctacctt agggttatta acatccataa cagatttaaa gtaaacctca
     3841 cccattttag caaattgaac taaataagaa ggtttagaat caacgttagc tcctgcatcg
     3901 attatcataa aaggaccatt tttacctggc ataactggtg ctaaagcagg tctttcaacc
     3961 ccttttattc tccctacaat aagtgtacat cctgttaaaa atgcacctgt gcttccagct
     4021 gatataactg cttcacactg attatcctta acaagtctaa gtgctttaac aagacttgag
     4081 tcttttttct ttttaacagc catagcagga tgctcattag tacttatcac ttctttagca
     4141 tctataaaaa taactctttc tttgtcatat ttaaacttag aaaaagcctc ttttaaaaga
     4201 tcttctggtc cagttatata tatttctata tccttaaatt cctctaatgc ctgaacacaa
     4261 ccttcaacta ctgcacttgg ggaatgatct ccccccattc catctacagc aattctcatg
     4321 agtttcaccc ctttttttcc tatatataaa gaaaagaaag tcataagact ttcttttatt
     4381 cttcaactga tactacttct ttacctttgt agtgtccgca gtttttacat actctatgag
     4441 ccattttcat ttcatgacat tgtgggcatt ctacgatacc tggtaagctc gccttgaaag
     4501 tttgagctct tcttgaatct ctttttgctc tgaacgtttt tctagctgga tttcccattg
     4561 ttacacctcc ttattactta aacaagtcca ttagctttgc cattcttaag tcaatttctt
     4621 cattgtcaca ttggcatgtt tctttattta aatcaatacc acatttttga caaagcccat
     4681 tgcagtctat actgcaaagt ctttttatag gtaaggttga aattacgttt tctactaccg
     4741 cttcagcaac atttaattcg tcgccttcta cataagcaat agaatcatct tcttgatttg
     4801 aattatttgt aaatttctca tccatatcta ttgatatttc ataagagaaa ttagttaagc
     4861 atcttgaaca cttcatctga agttctccag taatgttacc tactaactct ataaaagact
     4921 cattagatat aacttcacca gtaaaattta gaggctctat aaccttcata ttttcgcctt
     4981 caaattcgaa gctttttaaa tccagaataa aatctaaaga ttttcttctt tctctttttg
     5041 agcatatttc tgaaaaaatt aatttcataa tcatcgtagt caaataacta ctttcacgct
     5101 ccttaacata agatacgtat tgtttattcc taaacaacac atatcttatt atattaacta
     5161 gctatttaaa agtcaagtat tttataatta tttacttaca atacttaatg tatcttgcgc
     5221 tatcattaat tcttcattag ttggaactac gaaaagttta atttttgaac cctctttgct
     5281 tatttcagca actttagctc ttatattatt tctttcttca tctaactcaa ttcctaagaa
     5341 ctctaaatct ctacagatag ctcctctttc tatgattgag ttttctccaa caccagcagt
     5401 aaatactatt acgtcacatc cattcatctc tgcaatgtat gctcctatat atttcttaac
     5461 tgaatttctg aataaatcta aagctatttg agcttctttg cttcctttgt cagcagctgc
     5521 ttcaacatct ctaaagtcac tgcttactcc tgaaactcca aatattcctg attttttgtt
     5581 cattaaagta tttacttctt gtgaagtata tcctaattca tctattaagt atgtaactac
     5641 agctggatct atatttccac atctagttcc cattactaaa ccttcaagtg gtgtgaatcc
     5701 catagtagta tctacacatt taccattttt tatagctgct atacttgcac catttcctaa
     5761 gtgacaagtg attattttta aatcttctat gtttttaccc atagcttctg ctattttagc
     5821 tgaaacatat ttatgagaag ttccgtggaa accatatttt cttatgtgtt ctttttcata
     5881 taattcatat ggtaatgcat atacgaatgc tttttctggc attgtttgat ggaatgctgt
     5941 atcaaataca acaaccatag gaacatttgg cattaatgct ttacaagcat ttattcctat
     6001 tatatttgca ggattatgaa gtggagctaa tttagcacat tcttctaagt ctttcataac
     6061 ttcttctgta actaatgcag attcagcata tttttctcca ccatgaacaa ctctgtgtcc
     6121 tatagcattt atttcatcta atgattttat aacaccatgt ttttcatcta ttaaagctcc
     6181 tagaactaat ctgatagcat cttgatgatc tttcattggc tctatgattt catatttatc
     6241 tttaccttca ctcttttgag ttaagtttgc gccttctaat cctattcttt ctactaatcc
     6301 agttgctaaa gcattttcag tatccatatc tattaattga tattttaatg atgagcttcc
     6361 gcaatttata actaatacgt tcattataaa ctcctcctgt taaactataa ggtaaaatta
     6421 ataagcttaa ttcattttaa ttttatccat ctatataaaa ttctaatcaa ttactattta
     6481 gttgcttgtg cttgaacagc agttattgca acaacgttaa ctatatcctc tgaactacat
     6541 cctcttgaaa ggtcatttat tggtttagca aaaccttgac aaactggtcc aatagcttct
     6601 gcattagcaa atctttgaac taatttgtat cctatatttc cagcttgtaa atccgggaat
     6661 actaaaacat tggcatttcc tgctacttta ctatttggag ctttttgtga agcaaccttt
     6721 tcaactatag cagcatctaa ttgtaattca ccatctatat ctaaatctgg tcttaattct
     6781 ttagcttttt ctgttgctac tcttactttt gttactagat catggtcagc acttcccatt
     6841 gttgagaatg aaagcattgc aacctttgga tccattttgc aaagattttt agcagtatca
     6901 gcagtagcta tagctatagc agctaattgg tctgcatttg gatttggatt aacagcacag
     6961 tctgagaata ataacattcc accttctcca tattgtgaac ctggtaccat cattatgaag
     7021 aaacttgata ctacagatac tcctggagca gtttttatta tttgaagacc tggtcttaaa
     7081 aggtcaccag ttgtgtgtac agcacctgat accattccgt ctgcatcacc catttttacc
     7141 atcattgttc caaagtataa aggatctctt acgatttttc cagctttttc aactgtcatt
     7201 cctttagctt ttcttaattc ataaaattcg tttatataag tgtctaattt atctgaagtt
     7261 tcaggatcaa ctatgtttac accttctaaa tttactccta aagcttcagc tttttctctt
     7321 attacttgtt ctgaacctac taagtaaacg tcagcaagtc cttcttcttt tattctttgt
     7381 gaagcgacaa gtgttctctc ttcatcacct tctggaagta ctattttttt tctattattt
     7441 ttagcacttt cccatatttg tttcataagt tccatgttca tttctccttt caagtcaaag
     7501 cagatgttat gtgttattac acacaatact aataatacta tactcctata ctaatatatt
     7561 tttcaacaga taacaaacaa aaaaaacggt atcatactat tgtttttata tttaaaatta
     7621 tc
//
LOCUS       DAEVGI010000116         7593 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.116, whole genome shotgun sequence.
ACCESSION   DAEVGI010000116 DAEVGI010000000
VERSION     DAEVGI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.116"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(162..1607)
                     /locus_tag="K8M77_001952"
     CDS             complement(162..1607)
                     /locus_tag="K8M77_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI7086431.1"
                     /translation="MNDNKKLGVIALAGVVISAMLGGGVYNLPQNMAQSASAGAILIS
                     WIITGIGIWFIANTFRILAAARPDATTGIYTYGELGFGKFTGFLMAWGYWICNSFANV
                     GYAVLLMDSLNYFFPPYFKGGNNWLSIACGSLVLWIIFFIVLAGVKQASALNVIGTIG
                     KLLPLAIFLLVLLFSFKFSTFFTDFWGLKTVVKVHDTNLGGILPQVKSTMLVTLWVFT
                     GIEGAVVVSGRAKSQKDVSKATFLGFITCLLIYTLLSLLPLGVYSQGEISKMAPPSTA
                     AVLMDLIGNWGSVIMNLGVIIAILSSWLIWTVMLSQLPFAAAQSGTFPKIFAKENKNE
                     SPSFSLLASTIIMQIILILVHFAGNAWNMMLSITSVMALPCYLVSTLYLFKITYKNEN
                     YPTDIFAKRKYAMITAILGSIYGVWLIYAAGINYMLIAIVIYALGIPVFIKARCETSP
                     NEKEFTKVERYFAIVLIILALIGLVYLFKFM"
     gene            complement(1653..2615)
                     /locus_tag="K8M77_001953"
     CDS             complement(1653..2615)
                     /locus_tag="K8M77_001953"
                     /EC_number="4.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine decarboxylase, pyruvoyl type"
                     /protein_id="HBI7086432.1"
                     /translation="MNKNLEANRNRTLSKGIHKNIKVRAPKIDKTAISPYDRYCDGYG
                     MPGAYGNGYVSVLKVSVGTVKKTDDILLDGIVSYDRAEINDAYVGQINMLTASSFCGV
                     AGQVWGHDLAIHDSIANNEIKPLYELKQFDGTPLKVYDAKPLLDAGIELFGTEKNRRF
                     TTAPGAHVICANKSATAYRPKENRPLKEGEAYGVWSFIALSLSNDRDHCADLFIEDAG
                     LWKKNDNPEDLKKFLEDHRKAVTWSVVECGRDSHVVFERTYIGFAYVIMKPGEIGNAL
                     TCAPYVTLARDAIPSEGFPSLNRISLSQWLDDMNFDSLVNPSKK"
     gene            3083..3916
                     /locus_tag="K8M77_001954"
     CDS             3083..3916
                     /locus_tag="K8M77_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI7086433.1"
                     /translation="MEILLGALGITLVTGFFYYENNVISTTNYEVDCGIGKDINVVHL
                     SDLHGKEFGKNNEKLKMLILKEKTDLVVATGDMIDSSLKNMEGVIDFLSDLSKCVKVV
                     YISGNNEQRCKKAEYIFGSLKSKGVIVLRNEIITLSLSGVKVNILGMFEKPKGDLYSS
                     IKKINGSYAYEDSHKLFKRLESLEGLKIVLSHYPELFEAEYSKYDFHIMFSGHAHGGQ
                     FRIPIVKRGLIAPGQGFFPKYTEGMHGNKNKLIISRGLGNSTKITRLFNRPEIVNVKI
                     K"
     gene            4041..4193
                     /locus_tag="K8M77_001955"
     CDS             4041..4193
                     /locus_tag="K8M77_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086434.1"
                     /translation="MNKNMEMMKKLIEEKKNKGKNTKSNVRAQKTIGFAQGGRKSNNG
                     GGLFDK"
     gene            4508..5413
                     /gene="nadA"
                     /locus_tag="K8M77_001956"
     CDS             4508..5413
                     /gene="nadA"
                     /locus_tag="K8M77_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBI7086435.1"
                     /translation="MDIRDKILKLKKEKGAIILAHYYQIPEIQEIADYVGDSYYLSKI
                     AKDCEENIIVFCGVKFMAESAKILSPEKTVILPVMEAGCVMADMATEEGLAKLKEEHP
                     NAKVVCYINSSTEVKALSDVCCTSSNAENIINNLEEKEIIFLPDRNLGSYIQEKTPDK
                     KFILWNGFCIVHEAIQKEEILRLKREHEGILTVAHPECSKEIRDISDFIGSTSEIINF
                     VNNSSNKKFIIITEEGVLHQLRKNGEEKEFYIPYGKMVCRNMKMTTLKDLYESLLKME
                     NKIEIDEDLRLKAYNSLKNMHKLGG"
     gene            5418..6713
                     /locus_tag="K8M77_001957"
     CDS             5418..6713
                     /locus_tag="K8M77_001957"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBI7086436.1"
                     /translation="MKRVADVLIVGSGVAGLYASLNLREDLEIIMVSKKTVNLCNSSL
                     AQGGIAVARGKEDFHSFIEDTLKAGKYENNIDSVRVLVEESMNNINKLMDLGANFEKD
                     ENGVLFTKEGAHEINRIVYHKDITGKHVEDILLENVKRRKNIKIIEECEMVDIYHRDN
                     RCIGALFNKDEENLSIYAKVVILATGGIGGLFKKSTNERIITGDSIGIAIRNNIEIKD
                     LSYIQIHPTAFFSKKSEEKRFLISESVRGEGGKLLNCNGERFVDELLPRDIVAKKIYE
                     EMKKTNSNNVFLDVSFMEKSFLQNRFPNIYNKCLEEGIDISKEPIPVAPAQHYFMGGI
                     KVDLNGKTSMENLYAFGETSCTGVHGANRLASNSLLEALVFSRRGALEINNYIDNLEI
                     IIEEREYEDLDKYRLLNRKILIDEICRLRGDIKDELVTC"
     gene            6694..7533
                     /gene="nadC"
                     /locus_tag="K8M77_001958"
     CDS             6694..7533
                     /gene="nadC"
                     /locus_tag="K8M77_001958"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBI7086437.1"
                     /translation="MNWLLVEENVKRALNEDLQYGDITTESVVLDHKIAKVDIIAKEK
                     GIIAGTEVFKMVFKILGDVEVDFSVNDGEEVEKGQHFGQVSGDAKKILMGERVALNYM
                     QRMCGIATLTREFVERLEGTKVKLLDTRKTTPNMRIFEKYAVKIGGGTNHRFGLSDGV
                     MIKDNHIEAAGGIKNAVSLARKNSPFVRKIEVEVESIEQLKEALEAKADIIMLDNMDI
                     KTLKESVKLIDNRVEVEASGNVTLDNIREIAKTGVDFISTGSVTHSFKVLDISMKNFR
                     YID"
BASE COUNT         2952 a          874 c         1294 g         2473 t
ORIGIN      
        1 tttgtaaggt ttattgaagg ttataaaaaa aacacttttt tataaatgta agaaagctaa
       61 aaaaagactt tggttcattt tgagatattg atttattaat caagcttcaa aattcaccaa
      121 agtcttttaa attttggata gggttttgat atgtgagtag tttacataaa tttaaataaa
      181 tatactaagc ctattaaggc aagtattatc aaaactattg caaagtaacg ttcaacttta
      241 gtaaattctt tttcattagg gctagtctca catctagctt ttatgaatac aggaattcct
      301 aaagcatata taactatagc tataagcata tagttgattc cagcagcgta aattaaccaa
      361 acaccataaa ttgatcccaa tattgcagta atcattgcat attttctttt ggcaaatata
      421 tctgttggat agttttcgtt tttatatgtt atcttaaata aatataaggt agaaacaaga
      481 taacaaggaa gtgccataac acttgttata ctaagcatca tattccatgc attacctgca
      541 aaatgaacaa gtattaatat tatttgcata atgattgttg atgctaataa tgaaaatgaa
      601 ggtgattcat ttttattttc tttagcaaat atttttggaa atgttccgct ttgggctgct
      661 gcaaaaggca attgtgaaag cattacagtc caaataagcc aagaactcaa tattgctata
      721 attacaccaa ggttcattat tacacttccc cagtttccta ttaaatccat taatactgca
      781 gctgttgatg gtggagccat tttagaaatt tctccttgcg aatatacacc aagtggtaat
      841 aatgaaagta gtgtataaat taaaagacaa gtaataaatc ctaaaaatgt tgctttacta
      901 acatcttttt gagatttagc tcttccagaa acaacaacgg caccttcaat accagtaaat
      961 acccataagg ttacaagcat tgtgcttttt acttgaggca atattcctcc taagtttgta
     1021 tcatgaactt ttacaactgt ttttaatccc cagaagtcag taaagaatgt agaaaattta
     1081 aatgagaata ataaaactag taagaaaatt gctaaaggta ataatttccc tattgttcct
     1141 attacattta aagcacttgc ttgtttaaca ccagccaata ctataaagaa tataatccaa
     1201 aggacaagag atccacatgc tattgaaagc cagttattac cccctttaaa gtaaggtggg
     1261 aaaaagtagt ttagtgaatc cattaaaagt acggcatagc caacatttgc aaaagagtta
     1321 catatccaat atccccaggc cattaaaaat cctgtaaatt ttccaaaacc aagttcacca
     1381 taggtatata tacctgttgt ggcatcaggt cttgcagcag caaggattct aaaggtgtta
     1441 gctatgaacc aaattcctat cccagttatt atccaagata ttaatattgc acctgcagaa
     1501 gcggattggg ccatgttttg gggaaggtta taaacacctc caccaagcat agcactaatt
     1561 actactccag caagagcaat tacgcctaat tttttattat cattcataat aactcctcct
     1621 agaataattt tgttttaaga aattattatt tattattttt tactaggatt aactaaagaa
     1681 tcaaaattca tatcatctaa ccattgagaa agagaaattc tatttaaact tgggaaacct
     1741 tcagaaggaa tagcatctct tgctaaggtt acataaggag cgcaagttaa agcgtttcca
     1801 atttcaccag gtttcattat tacataagca aaacctatgt aagttctttc aaaaacaaca
     1861 tgagaatctc taccacattc aactactgac caagttacag cttttctatg atcttctaag
     1921 aatttcttta aatcttctgg attatcattt tttttccaaa gtccagcatc ttctatgaat
     1981 aaatcagcac agtgatctct atcatttgat agtgataagg ctataaatga ccatacacca
     2041 taagcttctc cttcttttaa aggtctattt tcctttggtc tataagctgt agcactctta
     2101 tttgcacata ttacatgggc acctggagca gttgtaaaac gtctattttt ttctgtacca
     2161 aaaagttcta taccagcgtc taataaaggt ttagcatcat aaactttaag tggagttcca
     2221 tcaaattgtt ttaattcata caaaggtttg atttcattat tagcaattga atcatgtatt
     2281 gctaaatcat gtccccaaac ttgacctgca accccacaga atgatgaagc tgtaagcata
     2341 ttaatttgtc caacataagc atcatttatt tcagctctat cataagaaac tataccatct
     2401 aataaaatat cgtcagtctt tttaactgta ccaacggaaa ccttaagaac tgatacatat
     2461 ccattaccat aagcccctgg cattccgtag ccatcacaat aacgatcata aggacttata
     2521 gctgttttat caattttagg tgctcttacc tttatatttt tatgtattcc tttagaaaga
     2581 gtcctgtttc tgtttgcttc aagattttta ttcatttaaa tcactcctaa acaatataag
     2641 tttaactatt aaatttttag aataaaaata tattatttgg ccaaataata atttaccttt
     2701 cattatgtaa aattatagca ctataaatat ttgttacaaa tttaatattt atagtattta
     2761 aaagttttca tatgaaattt tttttagttg tgtataaaag tgagctaaac tttaaaatat
     2821 taacagataa tgaatttaat gtatacaagg tttataaatt tctttatatt tttatgaaaa
     2881 aattttatgc ttattttaat taaatatatt taaagactta agtaattatt tgtgatagaa
     2941 taaaaatatt ttataagatt ataagtatat aaaatcttag aattttataa attaaaaata
     3001 ataaagagcc aaatttaata aaaattaata atataaataa aaatattatt ctgaaattaa
     3061 ttaagagtat aggtgtgata ttatggagat tttattaggc gcattaggta taaccttagt
     3121 aacaggattt ttttattatg aaaacaatgt aataagtaca actaattatg aagttgattg
     3181 tggaattggg aaagatataa atgtggttca tttatcagat cttcatggaa aagagtttgg
     3241 aaaaaataat gaaaaactta aaatgttaat attaaaagaa aagactgact tagttgttgc
     3301 cactggagat atgatagatt caagtcttaa aaatatggag ggggtaatag attttttaag
     3361 tgacttaagt aagtgtgtta aagttgttta tatatcagga aacaatgaac agaggtgcaa
     3421 aaaagctgaa tatatctttg gaagtctaaa gtcaaaggga gttatagttt taagaaatga
     3481 gattataact ttaagtttaa gtggagtgaa agtaaatatt ttaggtatgt ttgaaaagcc
     3541 aaagggagat ttatattctt caattaaaaa aattaatgga agttatgcat atgaggattc
     3601 ccataaactt tttaaaaggt tagaaagctt agagggatta aaaattgttt taagtcatta
     3661 tccagaactt tttgaggcag aatattcaaa atatgatttt catataatgt tttctggtca
     3721 tgcacatgga gggcaattta gaattcctat agttaaaaga ggattaattg caccaggaca
     3781 aggatttttt cctaaataca ctgaaggaat gcatggaaat aaaaataaac ttattattag
     3841 tagaggtctt ggaaacagta ctaaaataac aagattattt aatagacctg aaatagtgaa
     3901 tgttaagatt aaataatagg taatttaaaa attaagtttt gtttaaaata tgcaacatat
     3961 ctatttaaac taggatgatt tattttaaag aatatattaa aatttattat atattatgaa
     4021 attaaaagga gaaatttgta atgaacaaga acatggaaat gatgaaaaaa ctaatagaag
     4081 aaaaaaagaa taaagggaag aatactaaat ctaatgttag agctcaaaaa actattggat
     4141 ttgctcaagg gggaagaaag agcaacaatg gtggaggatt atttgataaa taaagtctaa
     4201 ttaaagatgt ttttatagga ttgtataaga actaagaaat aaattgcctg tatcaaatat
     4261 aataataaaa cttacaaaca tatttggaga attattagat cttcttttca taatgtttgt
     4321 aagttttatc aaaaattatt ttggtatagc ttctttattt ttaaggtgtt tttatacagt
     4381 ctttttttat tacaattaac aaaatgaaaa caatatatat ataatgttaa tgaatattcg
     4441 tattttattg aaattggata aacatggtga tattataaat taaaatacat aaattggggg
     4501 agacaaaatg gatataaggg acaaaatatt aaagttaaaa aaagagaagg gcgctatcat
     4561 attagcacat tattatcaaa ttcctgaaat acaggaaata gctgattatg taggtgattc
     4621 ttattactta agtaaaatag caaaggattg tgaagaaaat ataatagttt tttgtggggt
     4681 taaatttatg gcagaaagtg caaagatact ttcaccagaa aaaacggtca tattaccagt
     4741 tatggaagca ggatgtgtta tggcagatat ggctacagaa gagggactag ctaaattgaa
     4801 agaggaacat ccaaatgcca aggttgtttg ctatataaat tcttcaacag aagtaaaagc
     4861 attatctgat gtttgttgta cttcatctaa tgctgaaaat ataataaata atttagaaga
     4921 aaaagaaatt atatttttac ctgatagaaa tttagggtct tatattcagg aaaaaacacc
     4981 agataaaaag tttatattat ggaatggctt ttgcatagtt catgaagcta ttcaaaaaga
     5041 agaaatttta agattaaaaa gggagcatga agggatttta acagtagctc atccagagtg
     5101 tagtaaggag ataagagata tttcggattt cataggaagt actagtgaaa taataaattt
     5161 tgtaaataat tcttcaaata aaaaatttat tataataaca gaagaagggg tacttcatca
     5221 gttgagaaaa aatggagagg aaaaagaatt ttatattcca tatggaaaga tggtttgtag
     5281 aaatatgaaa atgactacct taaaggatct ttatgaaagt cttttaaaaa tggaaaataa
     5341 aattgaaata gatgaggatt taagattaaa agcatataat tcattaaaaa atatgcataa
     5401 acttgggggc taaaactatg aaaagggtag ctgatgtatt gatagttgga agtggggtag
     5461 caggactata tgcctcatta aatttaagag aagatttaga aataattatg gtttcaaaaa
     5521 agacagttaa tctatgcaat tcttccctag ctcagggtgg aattgctgta gctagaggaa
     5581 aagaagattt tcattcattc attgaagata ccttaaaagc tggaaagtat gaaaataata
     5641 ttgattctgt tagggtttta gttgaagaat caatgaataa tataaataaa cttatggatt
     5701 taggggctaa ttttgaaaag gatgaaaatg gagttctttt tacaaaagaa ggtgcacatg
     5761 aaataaatag aattgtttat cacaaagaca tcactggaaa gcatgttgaa gatattctct
     5821 tagaaaatgt taaaagaaga aaaaatataa aaataataga agaatgtgaa atggttgata
     5881 tatatcatag agataataga tgtataggag ctttatttaa taaagatgaa gaaaatttaa
     5941 gtatttatgc taaagttgta attttagcca caggtggaat tggtggttta ttcaaaaaat
     6001 ctactaatga aagaatcata actggagata gcatagggat tgcaattaga aataatatag
     6061 aaattaagga cctttcttat atacaaattc accctactgc atttttctca aaaaaatcag
     6121 aggaaaaaag atttttaatt tctgaatctg ttaggggaga aggaggaaaa ctcctaaact
     6181 gtaatggaga aagatttgta gatgagttat taccaaggga tatagttgct aaaaaaatat
     6241 atgaagaaat gaaaaaaaca aattctaata atgtattttt agatgtgtcc tttatggaaa
     6301 agagtttttt acaaaatagg ttccctaaca tatacaataa gtgcttagag gaaggaattg
     6361 atataagtaa ggagccaata cctgtagccc cagctcagca ttatttcatg ggaggaatta
     6421 aggttgattt aaatggaaaa acttctatgg aaaatttata tgcttttggt gagacaagct
     6481 gcacaggagt tcatggtgct aatagacttg ctagtaattc tttattagaa gctttggttt
     6541 tttcaagaag aggggcctta gaaattaata attatataga taatttagag attattatag
     6601 aagagagaga atatgaagat ttagataaat acagattgtt aaacaggaaa atattaattg
     6661 atgaaatctg taggctgaga ggagatatta aggatgaatt ggttacttgt tgaggagaat
     6721 gtaaaaagag ctcttaatga ggatttacaa tatggagata taacaacgga gtcagtagta
     6781 ttagatcata aaatagctaa ggttgatatt atagctaagg aaaaagggat aatagcaggt
     6841 acagaagtat ttaaaatggt ctttaagatt ttaggagatg tagaagttga tttttcagta
     6901 aatgatggag aggaagttga aaaagggcaa cactttggtc aggtttctgg agatgcaaaa
     6961 aaaattctta tgggagaaag agttgctcta aattatatgc aaagaatgtg tggaatagca
     7021 acccttacta gagaatttgt tgaaagatta gaaggaacaa aagttaagct tttagacaca
     7081 agaaaaacta ctcctaatat gaggattttt gagaaatatg cagtgaagat tgggggagga
     7141 actaatcata gatttggatt aagtgatggt gttatgataa aggataatca tatagaggca
     7201 gcaggtggaa taaagaatgc agtttcccta gcaagaaaaa attctccttt tgtcagaaaa
     7261 atagaagttg aagttgaaag cattgagcaa ttaaaggaag ctttagaagc aaaggcagat
     7321 ataataatgt tagataatat ggatataaaa acattaaaag aatctgtgaa attaatagat
     7381 aatagagtag aggtagaggc ttcaggaaat gtaaccttag ataatattag ggaaatagcc
     7441 aaaacgggag ttgattttat atcaacaggg tcagttaccc attcttttaa agtattagac
     7501 ataagcatga aaaacttcag atatatagat taaaaataag ttttatttgt atattatgga
     7561 ttaattgtaa aatgaaattg aactaaataa aaa
//
LOCUS       DAEVGI010000117         7523 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEVGI010000117 DAEVGI010000000
VERSION     DAEVGI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.117"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            51..1871
                     /locus_tag="K8M77_001959"
     CDS             51..1871
                     /locus_tag="K8M77_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBI7086438.1"
                     /translation="MGKVYIYHDSQGTFYREPFGAVSVGSKVSLRLECKECGEVFIEV
                     IKFDGSRYLIPMTIEERRNECIIYKGIIDTTNSLGVINYYFKYIKDGFTKYYGNNDEC
                     LGGEGKIYYDFPNYYQITVYEDNKISSWYKEGIIYQIFVDRFFNGNKDSMILNKKKNS
                     FIYGNWYDEPMYIRDSNGNIKRWDFYGGNLRGVIEKLDYIKSLGVNIIYMNPIFDAVS
                     CHKYDTGDYENIDKMYGTNSDFNELCQKAEEKGIRIILDGVFSHTGSDSRYFNKYGNY
                     GELGAYESKYSKYYNWYRFYDYPNSYECWWGFENQPNVEELEKTYSDYIVNSENSIIA
                     KWLRLGASGWRLDVADELPDEFIQMIKERMKNEKEDSVLIGEVWEDASNKVSYSKRRK
                     YLLGNELDSVTNYPYRDIISNFLNEEISSKDFYKVIMSIKENYPRENFFANMNILGNH
                     DTERILTVLKENLNKLKLALCLQMTLPGVPLIYYGDEAGLLGNKDPENRKTYPWGREN
                     KEILSYYSFFGNFRKNEEVLRKGDFYIFKDTPEDIIAFKRVYKDKEMIIIVNRSNSRK
                     TITLDSEKRRYKDKFSKEEFYGDGSITLEVERENYKILTN"
     gene            1926..2369
                     /locus_tag="K8M77_001960"
     CDS             1926..2369
                     /locus_tag="K8M77_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086439.1"
                     /translation="MIRTIVCQRDGCNGNAFYINSHDGEMSVVCKECNSEYKYEIENN
                     SLLMLSTCSNCNNDTFKVFKDTESNNIYAKCIVCGNLPENIFIDADGNQVSYESKILN
                     DIKDMVYRVEQRISDLEREAESLGSGQVLIEQSIAYINQFLSENK"
     gene            2594..3760
                     /locus_tag="K8M77_001961"
     CDS             2594..3760
                     /locus_tag="K8M77_001961"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBI7086440.1"
                     /translation="MSKKEIVAMILAGGQGSRLGVLTKNLAKPAVPFGGKYRIIDFPL
                     SNCSNSGIYTVGVLTQYKPLKLNEHIGIGDTWDLDRRDGGVSILPPYQKEKGGDWYKG
                     TANAIYQNIEFIERYDPEYVLILSGDHIYKMDYDKMLEMHKQKEADATIAVINVPMHE
                     ASRFGIMNTNEDLSIYEFEEKPEHPKSTNASMGIYIFNWKILKKFLEEDELDPSSSND
                     FGKNIIPKMLNSGKKLIAYPFNGYWKDVGTIESLWEANMDLLKYEDELSLYDSEWKIY
                     SANPVRPAQFIGKDAEIKSSLTVEGCIVHGKVENSVLFQGVYVGKGAIVKDAVIMPNT
                     KIEDNVLIEKAIIGSEAIVCKGCKIGDGNKISVIASKEVVIGSKEIIEECAMVK"
     gene            3790..4896
                     /gene="glgD"
                     /locus_tag="K8M77_001962"
     CDS             3790..4896
                     /gene="glgD"
                     /locus_tag="K8M77_001962"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBI7086441.1"
                     /translation="MNNCIGIINLDENESKIGELVKNRPLAAVPIAGRYRIIDFILSN
                     MTNAGIESLGILSKNKSRSLVDHLTNGRPWDLHRKRDGLRVFNFGERDPYYDDITNLY
                     ENLEFLTKSRREYVIMSPSYMICNIDLKEALEYHKKNDNDITVIYKKVKDADSNFIDC
                     DVLNFDECGFIKSIGKNLGREEESSISLEMYILRTDLLIEIIYECIRRGSHRKFKSYL
                     KEFLDEIKVGGFEFKGYLSCVNSLKSYYKANMDLLEEKVYKELFIDNNPIYTKSRDEG
                     PTHYTKNSEVTNSIIANGSYIEGKVENSIIGRRVYVGPEAVIKNCIIMQETVIGENAH
                     LDKIITDKAAVIRDDDKIVGLEYCPMVLSKPNVI"
     gene            4959..5405
                     /locus_tag="K8M77_001963"
     CDS             4959..5405
                     /locus_tag="K8M77_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBI7086442.1"
                     /translation="MENKRKRIIAWSLMILWMAFIFFMSSQPGEVSSKQSNFVVVLLD
                     MFGMNLNSRFGELSTYIIRKGAHFTEYMILYFLAFNLLRLYITRKKAYIYSLIIVFGY
                     ACSDEFHQLFVEGRSGQFKDVLIDTSGGIFGSLLVILKNKIFSNKA"
     gene            complement(5572..6984)
                     /gene="cls"
                     /locus_tag="K8M77_001964"
     CDS             complement(5572..6984)
                     /gene="cls"
                     /locus_tag="K8M77_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI7086443.1"
                     /translation="MAFIINVILSVIIVVLERKQPEKTIAWLLILTLLPPIGLILYIF
                     LGRNWKRNKLNDRVNPEINELINPILDEYPEKQYVPLMELLAFNSDSPIFVNNDIKIY
                     KDGVEKFKDLKEELLKAKHHIHLEYYIVNSDEIGNEIKDILIKKALEGVKVRFIIDKV
                     GSSSLKRSYIKDLKKAGISVIMYSYFLAPLLKVINTQINYRNHRKIVVIDGQVGFLGG
                     INIGDEYLGRNKKFGYWRDTHIMIKGDFVLALQAVFLDDFITIEKANNSYTFYDKEFD
                     KYFPENIVAKERVLMQLVKSGPDSTFPAIMQSVLKMIMTARENIYITTPYFVPPSSII
                     EALRIASLSGIDVKIIFPEKSDHFMVNKASKSYLAELMDCGIEVYFYDKSSFIHSKTM
                     TIDGKICTLGTANMDIRSFELNYEINTVIYDKEITCKLDALFFEDLLKSRHFKIEEYE
                     KSTKFDKFIEGFARIFSSLL"
BASE COUNT         2923 a          718 c         1287 g         2595 t
ORIGIN      
        1 ttattttgat acacgccctt tgttttatta agtaagaaag gggtattgcc atgggaaaag
       61 tttatattta tcatgattct caaggtacct tttataggga accttttgga gcagtttctg
      121 taggttctaa ggtaagttta agattagaat gcaaagagtg tggagaagtt tttatagagg
      181 taataaagtt tgatggaagt agatatttaa tacctatgac aattgaagag agaagaaatg
      241 aatgcataat ttataaagga ataatagaca ctacaaactc cttaggagta ataaattatt
      301 actttaaata cattaaggat ggatttacta agtattatgg aaataatgat gagtgtttag
      361 gaggagaggg aaagatatac tatgattttc ctaattatta tcaaataaca gtttatgagg
      421 ataataaaat ttctagttgg tataaggaag gtattattta tcagatattt gtggatagat
      481 tttttaatgg aaataaggat agtatgatat taaataaaaa gaaaaatagc tttatatatg
      541 gtaattggta tgatgagcca atgtatataa gagatagtaa tggaaatatt aaacggtggg
      601 atttttatgg agggaattta aggggagtaa ttgaaaaatt agattatata aaatctttag
      661 gggtaaatat tatatacatg aatccaatct ttgatgctgt gagttgtcat aaatatgata
      721 ctggagatta tgaaaatatt gataagatgt acggaactaa cagtgatttt aatgaattgt
      781 gccaaaaagc tgaggaaaaa ggtataagga taatattaga tggagttttt agtcatacag
      841 gatcagatag taggtacttt aataaatacg gaaactatgg agagctggga gcctatgaat
      901 ctaaatactc taaatattat aattggtata ggttttatga ttatcctaat agttatgaat
      961 gttggtgggg ttttgaaaac cagcctaatg tagaggaatt agaaaagaca tattcagatt
     1021 atatagttaa tagtgaaaat tcaataatag cgaagtggct tagattagga gcaagcggat
     1081 ggaggttaga tgtagcagat gaacttccag atgaattcat acaaatgatt aaggaaagga
     1141 tgaaaaatga gaaagaagat agtgtgctta taggagaggt ttgggaagac gcttcaaata
     1201 aggttagcta ttcaaaaaga agaaagtatt tattaggaaa tgaattagat tctgtaacaa
     1261 attaccctta tagagatata atttctaatt ttttaaatga agaaataagt tcaaaggatt
     1321 tttataaagt aataatgagc ataaaagaaa attatccaag agaaaatttt tttgcaaaca
     1381 tgaatattct aggtaaccat gacacagaaa gaatacttac agtattaaaa gagaatttaa
     1441 ataagttaaa attagcccta tgtcttcaaa tgactttacc tggagttccc ttaatttatt
     1501 atggtgatga ggcaggactt ttaggaaata aggatcctga aaatagaaag acctatcctt
     1561 ggggacgaga aaataaggaa atattaagtt attatagttt tttcggaaac tttagaaaga
     1621 atgaagaggt tttaagaaag ggagattttt atatttttaa ggatacacct gaggatatca
     1681 ttgcttttaa gagagtttat aaagataaag aaatgataat tatagtaaac aggagtaatt
     1741 ctagaaaaac cataacctta gatagcgaga aaagaagata taaagataaa ttttctaagg
     1801 aagaatttta tggtgatggt agcattactt tagaggtaga aagagaaaat tataaaattt
     1861 taactaatta gaggtatttt ctaagggaaa aaatcatatt aatccaatat atggaggtgg
     1921 ataatatgat aagaacaata gtatgccaaa gagatggatg caatggaaat gctttttata
     1981 taaatagtca tgatggtgaa atgagtgttg tatgtaagga gtgtaatagt gagtacaaat
     2041 atgaaattga gaataattca cttttgatgt tatctacttg ctcaaactgt aataatgata
     2101 cttttaaggt ctttaaagat actgaaagca ataatatata tgcaaaatgt attgtttgtg
     2161 gtaatctacc agaaaatatt tttattgatg ctgatgggaa tcaggtatct tatgaatcta
     2221 aaattttaaa tgatataaag gacatggttt atagagttga gcaaagaata agtgatcttg
     2281 agagagaggc tgagagttta ggaagtggtc aagtattgat agagcaatct attgcatata
     2341 taaatcaatt tttatcagaa aataaataaa tttttaatta tgtactagga caatttctag
     2401 tactctttat tgttaaataa ttaaaatttt ttaaagaaat tattttagtt atttaaataa
     2461 tttaaaacaa ttttttatta agattaaatt tattttaaat taattttaat aactattaaa
     2521 gttaaatata aagtatgtta taatttaaat atccatttaa tggtatataa ggggtaaatt
     2581 ggaggggaga tttgtgtcaa aaaaagagat agttgcaatg atattggctg ggggtcaagg
     2641 atctagatta ggtgtattaa ctaaaaattt agctaagcca gcagttcctt ttggaggaaa
     2701 atatagaata atagattttc ctttaagtaa ctgttctaat tcaggtatat atactgttgg
     2761 tgttttaaca cagtataagc ctttaaaatt aaatgaacat attggtattg gtgatacatg
     2821 ggatttagat agaagagatg gaggggtaag tatattgcct ccttatcaaa aagaaaaagg
     2881 cggagattgg tataaaggaa ctgctaatgc aatttatcaa aatattgaat tcatagaaag
     2941 atatgaccca gaatatgttc ttattttatc aggagatcat atttataaaa tggattatga
     3001 taaaatgtta gaaatgcata aacaaaaaga agctgatgct actatagcag taataaatgt
     3061 tcctatgcat gaggctagta gatttggaat aatgaataca aatgaagatc tatcaatata
     3121 tgagtttgaa gaaaagccag aacatccaaa gagtacaaat gcttcaatgg gaatatatat
     3181 atttaattgg aaaattttaa agaaattttt agaagaggat gagttagatc caagttcaag
     3241 caatgatttt ggaaaaaaca taattcctaa aatgttgaat tcaggaaaaa aacttatagc
     3301 atatccattt aatggttatt ggaaagatgt tggaactata gagagtttat gggaagctaa
     3361 tatggattta ctaaagtatg aagatgaatt aagtctttat gatagtgaat ggaaaattta
     3421 ttctgcaaat ccagttagac ctgctcaatt tattggaaaa gatgcagaaa taaagtcgtc
     3481 attaacggtt gaaggatgta tagtacatgg taaggtggaa aattcagttt tatttcaagg
     3541 tgtatatgtg ggaaaaggag ctatagttaa agatgctgta ataatgccaa atacaaaaat
     3601 tgaagataat gttttaatag aaaaagctat tataggtagt gaagctattg tttgtaaagg
     3661 atgtaaaata ggagatggaa ataaaatttc tgtaatagca tctaaagaag ttgttatagg
     3721 tagtaaagaa ataatagaag aatgtgctat ggtaaagtag gaaaatgttt atgagaggag
     3781 gaattattaa tgaataattg cataggtata ataaatttag atgagaatga aagcaaaatt
     3841 ggagaattag taaaaaatag gcctttagca gcagtaccta tagcaggaag atatagaata
     3901 attgatttta tactttctaa tatgaccaat gcaggaatag aatctttagg cattttaagt
     3961 aaaaataaat cgcgttcatt agtagatcat ttaactaatg gtagaccatg ggatttgcac
     4021 agaaaaagag atgggttaag agtatttaat tttggagaaa gagatccata ttatgatgat
     4081 ataactaatc tttatgaaaa tttagagttt ttaactaaaa gtagaagaga atacgttatt
     4141 atgtcacctt catatatgat ttgtaatata gatttaaaag aagctttaga atatcataag
     4201 aaaaatgata atgatattac agtaatatat aaaaaagtta aagatgcaga ttctaatttt
     4261 attgattgtg atgttttaaa ttttgatgaa tgtggattta taaaaagtat aggaaaaaat
     4321 ttgggtagag aagaagaaag tagcataagt ttagaaatgt atatattaag aacagatctt
     4381 ctaatagaaa taatatatga atgtataaga agaggttctc atagaaaatt taaaagttat
     4441 ttaaaggaat tcttagatga aattaaggtt ggtggatttg aatttaaagg ttatttaagt
     4501 tgtgttaatt cactaaaatc ttattacaag gctaatatgg atttattaga agagaaggtt
     4561 tataaggaat tatttataga taataatcct atatatacta aatctagaga tgaagggccg
     4621 acacattata ctaaaaatag tgaagtaact aattcgataa tagcaaatgg ttcttatatt
     4681 gaagggaaag ttgaaaacag tataattgga agaagagttt atgtaggacc agaggcagtt
     4741 attaaaaatt gtattataat gcaagaaact gttataggtg aaaatgccca tttagataaa
     4801 ataataacag ataaggcagc ggtaataaga gatgatgata aaatagtagg tcttgaatac
     4861 tgtcctatgg ttttaagcaa acctaatgta atataaactt gaaataaatc atagcatcct
     4921 ttaaatttaa tttatacaaa aggagaagag gagatgctat ggagaataaa agaaaaagaa
     4981 taattgcatg gagtttaatg atattatgga tggcttttat attttttatg tctagccagc
     5041 caggagaggt ttcttctaag caaagtaatt ttgtagttgt tttacttgat atgtttggaa
     5101 tgaacttaaa tagtcgtttc ggtgaattat caacttatat tataagaaag ggagcacact
     5161 ttactgaata tatgatactt tatttcttag cttttaatct tttaagatta tatataacta
     5221 gaaagaaggc atatatatat tccttaatta tagtatttgg atatgcttgt agcgatgaat
     5281 tccatcaatt atttgtagaa ggaagaagtg gacagtttaa agatgttctt atagatacat
     5341 caggaggaat ttttggaagc ttattagtta ttttaaaaaa taaaattttc tctaataaag
     5401 cttaaaatag aaaaatgtta aaatatattt attttaaata caaaaatgac ctatattaaa
     5461 ataactttta acaaaactta caaacagtat gaagagaaaa cttgtagttc tctgaatatg
     5521 tttgtaagtt ttattattct aatacagaac ctttttctaa ttaataattt tttataataa
     5581 tgatgagaaa attctagcaa atccctctat aaacttatca aacttagttg atttttcata
     5641 ttcttcaatt ttaaaatgtc tagacttaag taaatcttca aaaaataagg catctaactt
     5701 acatgttatt tccttatcat atataacagt atttatttca taattaagtt caaaactacg
     5761 tatgtccata ttagctgttc ctaaagtaca tattttccca tctattgtca ttgttttaga
     5821 atgtataaaa gaacttttat cataaaaata aacttcaatt ccacaatcca taagttcggc
     5881 taaataactt ttagatgcct tatttaccat aaagtgatct gatttttcag gaaaaataat
     5941 ctttacatca atcccgctta aagaagctat tctaagagct tctattatgc ttgatggagg
     6001 tacaaaataa ggagtagtta tataaatatt ttcccttgca gtcataatca tttttaaaac
     6061 gctctgcatt attgcaggaa atgttgaatc aggtccgctt ttaactaact gcattaaaac
     6121 tctttcctta gcaactatat tttcaggaaa atatttatca aattctttat cataaaaagt
     6181 ataactatta ttagcttttt ctatggttat gaagtcatct aggaaaactg cttgtagtgc
     6241 taaaacaaaa tcacctttaa tcattatgtg agtatctctc caataaccaa actttttgtt
     6301 tcttcctaaa tattcatctc ctatattaat accacctaaa aaaccaactt gaccatctat
     6361 aacaactatt tttctatgat ttctatagtt aatttgagta tttattactt taagaagggg
     6421 tgctaagaag tatgaataca taataactga gattcctgct ttttttaaat ctttaatgta
     6481 gcttcttttt aagctacttg aaccaacttt atcaataata aatctaacct taactccttc
     6541 tagagctttc tttatcaata tatctttaat ttcattgcct atttcatcac tatttactat
     6601 atagtactca agatgaatat gatgcttagc cttaagaagt tcctctttta aatctttaaa
     6661 cttttcaaca ccatccttat atatctttat atcattatta acaaagatag gagaatcact
     6721 gttaaaagct aaaagctcca ttaaaggtac atattgtttt tcaggatatt catctaaaat
     6781 aggatttatt aactcattaa tctcaggatt aaccctatca ttaagtttat ttcttttcca
     6841 attacgtcct aagaatatat aaagtattaa tcctatagga ggcaatagag ttagtattag
     6901 taaccatgct attgtttttt ctggttgttt tctttctagt accacaatta tgacagacaa
     6961 gattacatta attataaatg ccaagtataa aaatgttgtt gtatagttta taataatccc
     7021 tccctcataa ataaaatact aaatcttatt gtaacattaa ttatatcaat aactaatgat
     7081 ttctaaacat tttatataag atttataaat aaaatttatt ttaattatgt gttggtgtag
     7141 taattttatt tcatgtttta atatcaagga gtgtgttggt tttatataaa ggaatagata
     7201 gagctataaa ttaagataaa gatttctatt aaacgtttgt taaatccagg tttaacaaga
     7261 tatattaatg ataggatata aaatctgatt aatactttgg tatgtattta aattaccttt
     7321 agtgcttgga gcagtaatag ttacataata tatgaatata aaaagttata aattatataa
     7381 agttataagt attatttata aagataatat aagtatgaaa aatatagaat tattttagat
     7441 agagtattat gatataggat ttaatacata actattaaat cctatatctt ttttaagttt
     7501 aaatttaaaa gggtagttat tga
//
LOCUS       DAEVGI010000118         7518 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEVGI010000118 DAEVGI010000000
VERSION     DAEVGI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.118"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            136..1800
                     /locus_tag="K8M77_001965"
     CDS             136..1800
                     /locus_tag="K8M77_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HBI7086444.1"
                     /translation="MKSKRFQVLSERPVNQDGLIGEWADEGLIALDSPNDPKSSIKIE
                     NGIITELDGRSRDEFDMIDKFIAEYAINIEDAEASMKLSSKEIARRLVDINVSRDEIV
                     KITTSITPMKAVEVIQEMNVVEMMMALQKMRARRTPANQCHVTNVKDNPVQIAADAAE
                     AALRGFAEQETTVGIVRYAPFNALAILVGSQVGRGGVLTQCAVEEATELDLGMRGLTS
                     YAETVSVYGTESVFTDGDDTPWSKAFLASAYASRGLKMRFTSGSGSEALMGYSEGRSM
                     LYLESRCIYITKGAGVQGLQNGAVSCIGMTGAVPSGIRAVLGENLIAAMLDIEVASAN
                     DQTFSHSDIRRTARMLMQMLPGTDFIFSGYSAVPNYDNMFAGSNFDAEDFDDYNILQR
                     DLKVDGGLRPVTEEETIKVRNKAAKCIQIIFRELGFPEVTDEEVEAATYCHGSKEMPN
                     RNVVEDLKAAEEMLERRITGLDIIKALSKNGMEDIANNLLNMLKQRVTGDYLQTSAIL
                     DKDFNVISAVNDVNDYMGPGTGYRLDGQRWEEIKKVPTVMRPEDIE"
     gene            1808..2380
                     /locus_tag="K8M77_001966"
     CDS             1808..2380
                     /locus_tag="K8M77_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBI7086445.1"
                     /translation="MTMEERTFIPEITVEEVGEAKVGLRSDEVVIGLAPAFLKYQNKT
                     IVDVPHTETLLEIIAGIEEEGLHARVVRVLRTSDVSFIAHDAACLSGSGIGIGIQSKG
                     TTVIHQKDLLPLNNLELFSQAPLLTPETYRLIGKNAAKYAKGESPTPVPVKNDQMVRP
                     KFMAKAALLHIKETKHVEPGKKPVQLEVKF"
     gene            2400..2825
                     /locus_tag="K8M77_001967"
     CDS             2400..2825
                     /locus_tag="K8M77_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HBI7086446.1"
                     /translation="MENKRMTAADYPLTSKRKGDIKTPTGKALEDITLEKVLSGEINA
                     DDIRISPETLEMQAQIAESMNRDAIARNFRRAAELIRVPDDRILEMYNALRPYRSTKE
                     DLFKIADELETKYDAKVNADFVREAAEVYETRNKLRIEE"
     gene            2842..4692
                     /locus_tag="K8M77_001968"
     CDS             2842..4692
                     /locus_tag="K8M77_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBI7086447.1"
                     /translation="MKIIAGIDIGNSSTETALGKVYENNVEFLSSGIIPTTGIKGTEE
                     NISGVIASLNQGLKKANLTLEDLDLVRINEAAPVIGDVAMETITETIITESTMIGHNP
                     STPGGLGIGIGKTIRLETLETLNIDEIKEEDNAFIPLVLGNISFLEAVFRINQATRRG
                     INITAAIVQKDDGVLINNRLDKKIPIVDEVSLLEKVPVNMKAAVEVAPQGSVIRQLSN
                     PYGIATIFDLSPEETKMIVPVSRALIGNRSAVVIKTPQGDVKEKKIPAGKINITGIRR
                     KESVDVEEGADKIMEAVSLCSPIEDLRGDAGSNVGGMLEKVRQVMADLTNQSISDIKI
                     QDLLAVDTFIPQKVKGGLAKEFSMENAVGIAAMVKAHKLQMQIIANKLEEKLGVPVEV
                     GGVEADMAIRGALTTPGTNTPLAILDMGAGSTDASIINKEGNITSIHLAGAGNMVTML
                     IKSELGIEDFGLAEDIKKYPLAKVESLFHIRHEDGTVEFFEKPLDSSVFAKIVIIKEG
                     MLIPVDGQNSLEKIKNVRKTAKERVFVINCLRALKSVSPTGNIRDIEFVVLVGGSSLD
                     FEVPELVTDALSHYGVVAGRGNIRGCEGPRNAVATGLVLAFDRKGVKEND"
     gene            4685..5035
                     /locus_tag="K8M77_001969"
     CDS             4685..5035
                     /locus_tag="K8M77_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HBI7086448.1"
                     /translation="MIKDYNHPSIFVYCSLGINEVDIEEILWGIEEEGIPFILKNKDL
                     NDAKELANLAANDSKLSVGIGVNSKGDVSLTINKLNEEEPLFFINLKEGNTCLRSLGA
                     NGARLVKGMPLKNI"
     gene            5054..5566
                     /locus_tag="K8M77_001970"
     CDS             5054..5566
                     /locus_tag="K8M77_001970"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI7086449.1"
                     /translation="MNIYTKTGDKGTTSLFGGSRVDKCDLRVDAYGTIDELISFIGLA
                     YAELTDEEEKEILNKIQKELFMLGAELASDEKGLELLKDKIQLENIEYLEELIDRYMG
                     IAGPLTAFVIPGKNKPSATLHVARTVARRGERIMTALNEVQPLREEIKKYINRLSDTL
                     FALARYYEEK"
     gene            5590..6018
                     /locus_tag="K8M77_001971"
     CDS             5590..6018
                     /locus_tag="K8M77_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBI7086450.1"
                     /translation="MKKLNDVKELSLEIVKEMAKAAEAKACEMNVPVIFAAVDAGANL
                     MLMHRMEDAFLTSIDIAINKAYTAACLKQGSHEIAECVQPGQSLYGLQLTNNCRIVPF
                     GGGFPIIVDGKVVGAVGVSGGTVEEDMAIAQAAVDCFNNK"
     gene            6112..7269
                     /gene="dhaT"
                     /locus_tag="K8M77_001972"
     CDS             6112..7269
                     /gene="dhaT"
                     /locus_tag="K8M77_001972"
                     /EC_number="1.1.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-propanediol dehydrogenase"
                     /protein_id="HBI7086451.1"
                     /translation="MRMYDYLVPSVNFMGANSISVVGERCKILGGKKALIVTDKFLRG
                     LKGGAVELTEKYLKEAGIEVAYYDGVEPNPKDTNVKDGLKIFKDENCDMIVTVGGGSS
                     HDCGKGIGIAATHEGDLYDYAGIETLTNPLPPIVAVNTTAGTASEVTRHCVITNTKTK
                     VKFVIVSWRNLPLVSINDPMLMVGKPAGLTAATGMDALTHAVEAYVSKDANPVTDAAA
                     IQAIKLISSNLRQAVALGENLVARENMAYGSLLAGMAFNNANLGYVHAMAHQLGGLYD
                     MPHGVANAMLLPHVCKYNLISNPQKFADIAEFMGENIEGLSVMDAAQKAIDAMFRLST
                     DIGIPAKLRDMGVKEEDFGYMAEMALKDGNAFSNPRKGNERDIVEIFKAAF"
BASE COUNT         2997 a          926 c         1510 g         2085 t
ORIGIN      
        1 tatttaaaat atgacaaaaa tttaacataa catatgaaga ttttccaatg gaatttgatt
       61 aaaattatat taaaatttag acatagacaa aaaaataaca aacgaatttg aatttgaaag
      121 tgaggagacg ttaaagtgaa atctaaaaga ttccaagtat tatcagaacg tcctgtaaac
      181 caagatggac ttataggaga gtgggctgat gaaggcttaa tagctttaga tagtccaaat
      241 gatccaaaat catcaataaa aatagaaaat ggaataatta ctgaattaga cggtagatca
      301 agagatgagt ttgatatgat agataaattt atagcagagt acgctataaa tatagaagac
      361 gcagaagcat ctatgaaact ttcatctaaa gaaatagcaa gaagattagt tgatataaat
      421 gttagtagag atgaaatagt aaaaatcact acttcaataa caccaatgaa ggctgtagaa
      481 gttattcaag aaatgaacgt tgttgaaatg atgatggctc ttcaaaaaat gagagcaaga
      541 agaacacctg ctaaccaatg tcacgttact aacgtaaaag acaacccagt tcaaatagca
      601 gcagatgctg cagaggctgc tttaagagga tttgcagagc aagaaactac agtaggtata
      661 gttagatatg caccttttaa tgcattagct atcttagtag gttcacaagt aggtagagga
      721 ggagttttaa ctcaatgtgc agttgaggaa gctactgaac ttgacctagg aatgagagga
      781 cttacaagtt atgcagaaac agtttcagtt tatggaacag aatcagtatt tacagatgga
      841 gatgatactc catggtcaaa agcattctta gcatcagctt atgcttcaag aggacttaag
      901 atgagattta catcaggttc aggttcagaa gcattaatgg gatactcaga aggtagatca
      961 atgctttact tagaatcaag atgtatatat ataactaagg gagctggagt tcaaggatta
     1021 caaaatggtg cagttagttg tataggtatg acaggggcag ttccatcagg aataagagca
     1081 gttcttggag aaaacttaat agctgcaatg cttgatatag aggttgcatc agcaaatgac
     1141 caaacattct cacactcaga cataagaaga acagcaagaa tgttaatgca aatgcttcca
     1201 ggaacagact tcatattctc aggatatagt gcagttccaa actacgataa catgtttgct
     1261 ggatcaaact ttgatgcaga agactttgat gattacaaca tacttcaaag agacttaaaa
     1321 gttgacggtg gattaagacc agttacagaa gaagaaacta taaaggttag aaataaagca
     1381 gctaaatgca tacaaataat ctttagagaa ttaggattcc cagaagttac tgatgaagaa
     1441 gtagaagctg caacttactg tcacggaagt aaggaaatgc caaacagaaa tgtagttgaa
     1501 gatttaaagg ctgcagaaga aatgttagaa agaagaataa caggattaga tataataaaa
     1561 gctttaagca aaaatggtat ggaagatata gcaaacaatt tattaaacat gcttaaacaa
     1621 agagttactg gagattacct tcaaacttca gcaatcttag ataaagattt caatgttata
     1681 agtgctgtta atgatgtaaa tgactatatg ggacctggaa caggatatag actagatggt
     1741 caaagatggg aagaaatcaa aaaagttcct acagtaatga gaccagagga tatagagtag
     1801 ggggagaatg acaatggaag aaagaacttt tataccagaa ataacagttg aagaagttgg
     1861 agaagctaag gttggtttaa gatcagatga agttgttata ggattagcac ctgcattttt
     1921 aaaatatcaa aataaaacaa tagtagatgt tcctcatact gaaactttac ttgaaattat
     1981 agcgggaatt gaagaagaag gattacatgc aagagtagtt agagttttaa gaacatctga
     2041 tgtatctttt atagctcatg atgcagcttg tttaagtgga tcaggaatag gtataggaat
     2101 acaatcaaaa ggtactacag taatacatca aaaagattta ttaccattaa ataacttaga
     2161 attattctca caagctccgc ttttaactcc ggaaacatat agattaatcg gaaaaaatgc
     2221 tgctaaatat gctaaaggtg agtcaccaac accagtacca gtaaaaaatg accaaatggt
     2281 tagacctaaa ttcatggcaa aggcagcttt actacacata aaagaaacta aacatgttga
     2341 accaggtaaa aagccagttc aattagaagt taagttttaa tttagaaagg attgaaatca
     2401 tggaaaataa gagaatgact gctgcagatt atcctttaac ttctaagaga aaaggagata
     2461 taaaaactcc tacaggaaaa gctttagaag atataacttt agaaaaagtt ttaagtggag
     2521 aaatcaatgc agatgatata agaatttcac cagaaacttt agaaatgcaa gctcaaatag
     2581 ctgaaagcat gaacagagat gctatagcta gaaactttag aagagctgcg gagcttataa
     2641 gagttccaga cgatagaata ttagagatgt acaacgcttt aagaccatat cgttcaacaa
     2701 aagaagattt attcaaaata gctgatgaac ttgaaacaaa atatgatgct aaagttaatg
     2761 ctgattttgt tagagaagca gctgaagtat atgaaactag aaataaatta agaatagaag
     2821 agtaaaatca gaggtgaaaa gatgaaaatc atagcaggaa ttgatatagg aaactcttct
     2881 acagaaactg ctttaggtaa ggtttatgaa aataatgttg aatttctgtc tagtgggata
     2941 attcccacta caggaatcaa aggaacggaa gaaaatataa gtggggtaat agcttcttta
     3001 aaccaaggtt taaagaaagc taacttaact ttagaagatt tagatttagt tagaattaac
     3061 gaagcagcac ctgttatagg ggatgttgct atggagacaa taactgaaac aataataact
     3121 gaatcaacaa tgataggaca taacccttct actccaggag gattaggtat aggaataggt
     3181 aaaactataa gattagaaac tttagaaact ttaaatattg atgaaatcaa agaggaagat
     3241 aatgctttta ttccattggt tttaggaaat ataagttttt tagaggctgt atttagaata
     3301 aatcaagcaa ctagaagagg tattaatata actgctgcca ttgttcaaaa ggatgatgga
     3361 gtattaatta ataacagatt agataaaaaa atacctatag ttgatgaagt ttctctttta
     3421 gaaaaggttc ctgtaaatat gaaagcagct gttgaagtag caccacaagg ttcagtaatt
     3481 agacagctat caaatccata tggaatagct actatttttg acttaagtcc agaagagaca
     3541 aaaatgattg ttcctgtatc tagagcctta ataggaaata gatcagctgt tgtaataaaa
     3601 actcctcaag gggatgttaa ggaaaagaaa ataccagctg gtaagattaa cataacagga
     3661 ataagaagaa aagagtctgt ggatgttgaa gagggagcag ataaaataat ggaggctgtt
     3721 agcctttgct ctccaataga agatttaaga ggagatgctg gttctaatgt tggaggaatg
     3781 cttgaaaaag ttagacaagt tatggctgat ttaactaatc aaagcatttc agatataaag
     3841 attcaggatt tattagcagt agatactttt atcccgcaaa aggttaaggg tggacttgca
     3901 aaagagtttt caatggagaa tgctgttgga atagcagcaa tggttaaagc tcataaactt
     3961 caaatgcaaa taatagctaa taaacttgaa gaaaagttag gagttccagt agaagttggt
     4021 ggagtagaag ctgacatggc aataagagga gctttaacaa ctccaggtac aaatactcct
     4081 ttagctattt tagatatggg agctggatct acagatgctt ctattataaa taaagaaggt
     4141 aacataacat caatacattt agctggtgca ggaaacatgg taactatgct tattaaatca
     4201 gaattaggca tagaagattt tggacttgca gaagatataa aaaaatatcc attagctaag
     4261 gttgagagtt tgtttcatat aagacatgaa gatggaactg tagagttctt tgaaaaacct
     4321 ttagattctt cagtttttgc taagattgta attattaaag agggaatgct tattcctgta
     4381 gatggacaga attctttaga aaagattaaa aatgttagaa aaactgctaa ggaaagagtt
     4441 ttcgtaatta actgtttaag agcattaaaa agtgtttcac ctacaggaaa tataagagat
     4501 atagaattcg ttgttttagt tggaggatca tcattagact ttgaggttcc tgaattagta
     4561 acagatgcct tatctcatta tggagtagtt gcaggaagag gaaatataag aggatgtgaa
     4621 ggtccaagaa atgcagttgc aactggacta gtattagcct ttgacagaaa gggtgtcaag
     4681 gaaaatgatt aaggattata atcatccaag catatttgta tattgttctt taggaataaa
     4741 tgaagtggat atagaagaaa ttctatgggg aatagaagaa gaaggaatcc ctttcatatt
     4801 aaaaaataaa gatttaaatg atgctaagga acttgctaat ttagctgcta atgattctaa
     4861 actttcagtt ggaataggag ttaatagtaa aggtgatgta agtttaacta ttaataagtt
     4921 aaatgaagaa gaacctttat tctttataaa cttaaaagaa ggaaatacct gtttaagaag
     4981 tttaggagct aatggagcaa gattagttaa ggggatgcct ttaaaaaata tttaaattga
     5041 agaggtgttt agcatgaata tttacactaa aactggtgat aaaggaacaa cttctctttt
     5101 cggaggaagt agagtagata aatgtgattt aagagtagat gcgtatggaa caatagatga
     5161 attaatatct ttcataggtc ttgcttatgc tgaattaact gatgaagaag agaaggaaat
     5221 acttaataaa atacaaaagg aattgttcat gttaggagcc gagcttgcta gtgatgaaaa
     5281 aggacttgag cttttaaaag ataaaatcca attagagaat atagagtatt tagaagaatt
     5341 aatagataga tacatgggca tagcagggcc tttaactgcc tttgttatac caggtaaaaa
     5401 taaaccttca gcaactcttc atgtagctag aactgtagct agaagaggag aaagaataat
     5461 gactgcttta aacgaagtac agccattaag agaagaaatt aaaaaatata taaatagatt
     5521 atcagatact ttatttgcat tagcaagata ttatgaagaa aaataattaa ccaaaaggag
     5581 gagaaaaata tgaaaaaatt aaacgatgta aaagaattaa gcttagaaat agttaaagaa
     5641 atggctaaag ctgctgaagc taaagcttgt gaaatgaatg ttccagttat atttgcagca
     5701 gttgatgctg gtgctaattt aatgcttatg cacagaatgg aagatgcatt tttaacaagt
     5761 attgatatag ctataaacaa agcttataca gctgcatgtt taaagcaagg aagtcatgaa
     5821 attgcagaat gtgttcaacc aggacaaagt ctttatggtc ttcaattaac aaataattgt
     5881 agaatagtac catttggtgg tgggttccca attatagttg atggaaaagt agtaggagct
     5941 gttggtgtaa gtggtggaac agttgaagaa gacatggcta tagcacaagc ggcagtagat
     6001 tgttttaata ataagtaata aaaagttaat aatatttaac ttatataata gattaaatat
     6061 ttaaaatata ttttaataga tataaaatgt ttgaaaatgg aggaaaatga aatgagaatg
     6121 tacgattatt tagtaccaag tgtaaacttt atgggagcta actcaatatc agtagttggt
     6181 gaaagatgta aaatattagg tggaaagaaa gctttaatag ttacagataa attcttaaga
     6241 ggattaaaag ggggagcagt tgaattaact gaaaaatacc taaaagaagc aggaatcgaa
     6301 gttgcttatt atgatggagt tgaaccaaat ccaaaagata caaatgttaa agatggttta
     6361 aaaatattca aagacgaaaa ctgtgatatg atagttacag ttggtggagg aagctcacat
     6421 gactgtggta aaggaatagg tatagctgca actcacgaag gagatcttta tgactatgct
     6481 ggaatagaaa ctttaacaaa tccacttcct ccaatagtag cagtaaacac tacagctgga
     6541 actgcaagtg aagtaactag acactgtgtt ataacaaaca ctaaaactaa agttaaattc
     6601 gttatagtaa gctggagaaa cttaccttta gtttcaatca atgacccaat gttaatggtt
     6661 ggaaaaccag caggattaac agctgcaaca ggaatggacg ctttaactca tgctgtagaa
     6721 gcatatgtat caaaagatgc taaccctgta acagatgctg ctgcaataca agctataaaa
     6781 ttaatatcaa gcaatttaag acaagctgtt gctttaggag aaaacttagt agctagagaa
     6841 aacatggctt acggttcatt attagctggt atggcattta acaatgctaa cttaggatat
     6901 gtacatgcta tggctcacca attaggcgga ttatatgata tgcctcacgg agtagctaac
     6961 gctatgttat taccacacgt atgtaaatac aacttaatat ctaacccaca aaaatttgct
     7021 gatatagctg aattcatggg agaaaacata gaaggattat cagtaatgga tgctgctcaa
     7081 aaagctatag atgcaatgtt cagattatca actgatatcg gaataccagc aaaattaaga
     7141 gacatgggag taaaagaaga agacttcgga tacatggctg aaatggctct taaagatggt
     7201 aatgcattca gtaacccaag aaaaggtaac gaaagagaca tcgttgaaat attcaaagct
     7261 gcattctaat aaaagattag ctttatagta aaaaaataat taaaaatata ataagtgcta
     7321 gctctaatta ttagagctag caaacaataa aaggcataaa tacaatagac tttttatata
     7381 aacttaataa acaaacaaca gtaaaaatta atatatgtaa atcggataaa tgaaataatt
     7441 aagttataaa tacccagtaa cttaaattaa aaatactagt ttaaaataat tttataatat
     7501 acccaattaa ataatttt
//
LOCUS       DAEVGI010000119         7505 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEVGI010000119 DAEVGI010000000
VERSION     DAEVGI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.119"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(100..1248)
                     /gene="fliB"
                     /locus_tag="K8M77_001973"
                     /pseudo
     CDS             complement(100..1248)
                     /gene="fliB"
                     /locus_tag="K8M77_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
     gene            complement(1446..2411)
                     /locus_tag="K8M77_001974"
     CDS             complement(1446..2411)
                     /locus_tag="K8M77_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HBI7086452.1"
                     /translation="MKYYLAPMEGITGFIYRNSYEKFFGGIDKYFAPFVVPNSSKSLK
                     TKELRDVLPENNKGINLVPQILTNDSEGFILTAKKLKDLGYNEINLNLGCPSGTVVGK
                     KRGAGFLAHREELDKFLEEIFKINHMKISIKTRLGMDKPEEFYELIKIYNKYPMEELI
                     IHPRTRQDFYGNKPNLEVFKDAISLSKNPVCYNGDIFTLEDHNKLVEEFKEVDKIMLG
                     RGILANPALMNEILNNEFMDKKVLKEFHDEIFSKYREVFNEDRNAMFRMKELWGYMIY
                     MFSNNKKYAKKIKKAQKVVDYNQAVTSLFLEQDILKGAGLFSSEE"
     gene            complement(2515..3885)
                     /locus_tag="K8M77_001975"
     CDS             complement(2515..3885)
                     /locus_tag="K8M77_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7086453.1"
                     /translation="MKNQYELKNEKIGRLILKYSAPAILSMMVTSLYNTVDRAFIGSI
                     KDIGALAISGLGVTMPLFTILGAFCVAISVGGSTNISIKLGEEKKEEAERILGNTFIL
                     SIVVSLIIMIFGFSFLEKILYFFGASKETIIYAKDYMRVILIGAWFNFPGFALNNAIR
                     AEGNPKLAGKIMIISCILNIILDPIFIFLFNMGIKGAALGTIICQFVVFLWTMYYFTL
                     GKSNLKLRIKGRILNKNILRAIILIALTPFFMELAAGFIHLITNRVLKDYGGDLAIGA
                     MTSITSIYLLFLMPVFGLSQGMQTIVAYNYGAKEYKRAKKTLLMTIIIATLILTLGLV
                     LIRIYPREFINIFTKDKELISIALNGLKIYTFALPTLGVSILGAVYFQSIGNVKKSIF
                     LSLLRQVILFIPIVFIIPRVYGLNGVWISQPLADILATILILIFLIKEFYIRDKSYAI
                     PIEESA"
     gene            complement(4262..4603)
                     /locus_tag="K8M77_001976"
     CDS             complement(4262..4603)
                     /locus_tag="K8M77_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBI7086454.1"
                     /translation="MDNDELLNMRFKICDNLQYEVDEEGIVTILEKQDHKIQRFFRKL
                     KFKIPMYKRTSLDEYGSEVFLQINGTNTVKEIGEALESRFGEKVHPLYERLLIFLNHI
                     YLNCKYIEKVE"
     gene            complement(4615..6531)
                     /locus_tag="K8M77_001977"
     CDS             complement(4615..6531)
                     /locus_tag="K8M77_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBI7086455.1"
                     /translation="MKKKLPKEAYGGVSGKDYVPYFTGKSKNGVNLPVLIIGIVLAIV
                     FAASTAYSGMKSGLTVAAGIPGAIIGSVFVGIFARSKGILGKNITQGMASGGESIASG
                     MIFVLPAIILIGSKFTFLEGVAAGVGGVLFGIGCLSLVYNYLIVEEHGKLMYPESMAI
                     SETLVASEGSEDSIKFMGIGFVISGIINVLTGSFLNLINNTVTYLGSKFYKWKFSTEV
                     NPLLLGIGFIVGLEVSLTMFAGSILANFGIAPLIGYFSDMAEKSMHSWNDASMLIHQM
                     DVNAISGSYVKYIGAGMMLCGGIIGAIKLIPTIIVSIKETLKASPVNEENAEKNSGSI
                     IILLVGALLAFVIGFFISNSIAMAITGAVVSIILSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVILTLVFVIMGWTGKADNKALLLFATFMVVSISVAGGYSQSQKVTYIIGGSK
                     KEMQNSFMIASIVGVVVVTGTILVLSSQLSITGADAPFALPQANLMSTLTSGIMEGNL
                     PWPMIIVGVVMALFLYLVDLPIMTVAIGFYLPISTTSIILVGALIRVFIEKMSKSEHE
                     KEVKVSNGISLSSGLVAGGSIVGLIGIVLQVTGIITPGTPTGFVASNGMAIIVLILLV
                     LATTVPIMKSKVKH"
     gene            complement(7333..7485)
                     /locus_tag="K8M77_001978"
     CDS             complement(7333..7485)
                     /locus_tag="K8M77_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086456.1"
                     /translation="MGNKNRNSKKKMASLGLRKDENGDYKYIDPSDTTSEFVPVRNHG
                     RKSSHN"
BASE COUNT         2610 a         1304 c          719 g         2872 t
ORIGIN      
        1 cttaaatatg gtatttaaat aaaaaatttt aagttattgt taacttatat atgtaaactt
       61 atacatagtt ttaaatctct tcttagttta aaatacaatt tagaccaaac ttataaaaca
      121 tagataacct atttctaaaa ttatctcctc aaagccatca tttaagaact ctataacagc
      181 atctttatta ttttcaataa ctcttgaaaa agtaataata ttattcttta tctcttccct
      241 ttatattttt tcatcattaa tacaataatt taaaaataca gaatatcttg ttaataaata
      301 ttctaatata attaattgga aagaaattat catatcttcc atatcatcac ttgtacaatt
      361 actaaatatc ttagatataa tacatttttt aagtaaatct tcaaattctt taaatgattc
      421 tttatatttc tcccactttt cacttaaaag atttatatta gtattttcag caaaatcaga
      481 tactctttct agtacacact ttagattaga aatactctta taattttcta taatatctaa
      541 aaacagagag tttacttcct ctaatgattc acctttattt atttttattt cattatatag
      601 ataaatcact tcttttatgt actctatgtt actatacttt tctagttcat ctaagaaaat
      661 atcttcacta gtataacttt cattttctaa tatatttaac aacatctcaa aacctaaaag
      721 taatttttct tctaaggaaa atccctcttc atacattata tttattaaag tatccctcaa
      781 tttaagctct aatggtatat cttctttatt tattttttcc aattgattta ttataatctc
      841 actatctata ttatctaata tttccataac ttctggacat gaacatgata aagaaagctc
      901 ttttctatat tcaaactcat tacttattct tggaaatgta ctacaagttt tagataaata
      961 ctcttcccct ttacacttaa ctatattaca cagtccctta caatctaaaa aagaacacgc
     1021 ttcaaaggta tctttgttta taaagtataa tttttcccca ttaacttctc tcacttttac
     1081 attatttaaa aggtatttag aatgttcttt attatttttc catttttcat atgtatcctt
     1141 atctatatca atatcccatc cttcacaaca agtgaattta cagctatcag ctatacactt
     1201 aaaatcctca taacctgaaa gtttcttaat aatcttttct tcattcatat aatttctctc
     1261 catattttca ttatattagt taaaagtcta attactctaa taaatgtaat tatagcacac
     1321 tttctaattt actatttttt actatactat tcttattcct ataataaaaa acctaccaga
     1381 caataaaact aaagtctgat agattttaaa ataaatatat taaatataaa gaacatatct
     1441 tatatttact cttcactact aaatagtcca gctcccttaa gtatatcctg ttctaaaaat
     1501 aggcttgtaa cagcttggtt ataatcaacc actttctgag ctttctttat tttcttagca
     1561 tattttttat tatttgagaa catatagatc atatatcccc atagctcctt cattctaaac
     1621 attgcatttc tatcttcatt aaatacttct ctgtacttac taaatatttc atcatgaaac
     1681 tcttttaata cctttttgtc cataaactca ttgtttaaaa tttcattcat taaggctgga
     1741 ttagctaaaa tccctcttcc taacattatt ttatccactt ctttgaattc ttcaaccaat
     1801 ttattatgat cctctaaggt aaaaatatct ccattataac aaactggatt cttacttaaa
     1861 gaaattgcat ctttaaaaac ctctaaatta ggtttatttc cataaaaatc ttgtcttgtt
     1921 ctaggatgaa taataagctc ctccatagga tacttattat aaatttttat aagttcataa
     1981 aattcctcag gtttatccat tcctaatcta gtttttattg aaattttcat gtgatttatt
     2041 ttaaatattt cttctaaaaa cttatcaagc tcttctctat gagctaaaaa ccctgcccct
     2101 cttttcttac caacaactgt tccagaagga catcctaaat ttaaatttat ttcattatat
     2161 cctaaatcct taagcttttt agctgttaag ataaaccctt ctgaatcatt ggtaagtatt
     2221 tgtggaacta agtttattcc tttattattc tctggcaaaa catctcttaa ttctttagtt
     2281 ttaagacttt tactactatt tggaacaaca aaaggggcaa agtatttatc aattcctcca
     2341 aaaaacttct cataagaatt tctatatata aatccagtaa tcccttccat tggtgctaaa
     2401 taatacttca tttgtttctc ctttttttaa agattctaga aaatattata acaggctttt
     2461 ttcttcttat ccacttagta attaaagtat ttttatgtaa acttctttaa ttagctatgc
     2521 actttcttct atgggaattg cataactttt atctcttatg taaaattctt ttattaaaaa
     2581 tataagtatt aatatggtag ctaaaatatc agctaagggt tgtgaaatcc acactccatt
     2641 tagtccatat actctaggta ttataaatac aataggtata aatagtataa cttgtcttaa
     2701 taaacttaaa aaaattgact ttttaacatt tcctatagac tgaaaatata ctgcccctaa
     2761 aattgaaacc cctaaggtag gtaaggcaaa ggtatatatt tttaacccat taagtgctat
     2821 acttattaac tccttatcct ttgtaaatat attgataaac tctcttggat aaattctaat
     2881 aagtacaagt cctaaggtta aaattaaagt tgcaattatt atagtcatta ataaagtttt
     2941 tttagcacgt ttatactcct tcgctccata attatatgca actatggtct gcattccttg
     3001 acttagtcca aagactggca tcaaaaacaa aagatatata gatgttatgg atgtcattgc
     3061 tcctattgct aaatctccac cataatcctt aagcactcta tttgttatta aatgtataaa
     3121 tcctgctgca agctccataa aaaacggagt taaagcaatt aagattattg cccttaaaat
     3181 attcttattt aatatccttc ctttaatcct tagctttagg tttgactttc ctaaggtaaa
     3241 ataatacatt gtccaaagaa aaactacaaa ttgacagatt atagttccta aggcagctcc
     3301 cttaattccc atattaaata agaatataaa tattggatct aaaattatgt ttaaaataca
     3361 gcttattatc atgattttac cagctaactt tggatttccc tcagctctta ttgcattatt
     3421 aagagcaaat ccaggaaagt taaaccatgc ccctataagt attaccctca tataatcctt
     3481 tgcatatata atagtttcct tacttgcccc aaaaaaatat agtatttttt ctaaaaatga
     3541 gaatccaaag atcataataa ttaaagaaac aactatggat aatataaagg tatttcctaa
     3601 tattctttca gcttcttcct tcttttcttc ccctagcttt attgatatat tagtacttcc
     3661 tcctacagag atggcaacac agaaagcacc taatattgta aacaagggca tagttactcc
     3721 taagccagat atggcaaggg ctcctatatc cttaatagat cctataaaag ctctatctac
     3781 agtgttatat aaggaagtta ccatcataga tagtatagct ggggctgaat attttaaaat
     3841 taatctccct attttttcat tctttagttc atattgattt ttcataataa tgtccctatc
     3901 ctaatgtcct atatttctta tgatgaataa tcatttcatt tgttattaat aattattatc
     3961 aagtcttatt tattatagca tctacttatt cttagtcaat attttctgtg catattttta
     4021 aaaatattaa atatttattc cataacttaa aaataataaa tatgattata gggatgcttt
     4081 gttataaact taaaatatct ttccttattt aagataagaa aagattaata attatagtta
     4141 aggattcaaa cctcccttaa gttaaatatt ttcaaaatta caatttactt aaaatatatc
     4201 ctaaaattaa aataacctac aaaaatctgt aaaaagactt ttgtaggtta aaagataaaa
     4261 tctattctac tttttcaata tacttgcaat ttaaatatat gtgatttaga aaaattaata
     4321 gtctttcata aagaggatgt actttttcac caaacctaga ctctaaagct tcaccaattt
     4381 ccttaacggt attagttcca ttgatttgta aaaatacttc gctaccatat tcatctaaag
     4441 aagttctttt atacattgga atcttaaatt tcaattttct aaaaaatctt tgaatcttat
     4501 gatcttgttt ttctaaaata gtgactatgc cctcttcatc aacttcatac tgaagattat
     4561 cacagatttt aaatcgcata tttaaaagtt catcattatc cattataatt caccttaatg
     4621 tttcacctta gatttcataa taggtactgt agttgctaaa actaaaagga taagtactat
     4681 tattgccatt ccattgctag ctacaaaacc agttggtgtt ccaggtgtta ttattcctgt
     4741 tacttgtaga actattccta ttaatcccac aatagatcct ccagcaacaa gaccagaaga
     4801 taaacttatt ccatttgaaa ctttaacttc tttttcatgc tcactcttgc tcattttttc
     4861 aataaataca cgtattaatg cacctactaa aattatagaa gttgttgata taggtaaata
     4921 gaatcctatt gcaacagtca ttattggtaa gtctactaag tataagaata aagccataac
     4981 tactccaaca attatcatag gccaaggtaa gtttccttcc attatacctg aagttaatgt
     5041 tgacattaag ttagcttgtg gtaatgcaaa aggtgcatct gctcctgtta ttgataattg
     5101 gctagaaagt actaatattg ttccagtaac aacaacaaca ccaactatac ttgcaatcat
     5161 gaaagagttt tgcatttctt ttttgcttcc accaattata tatgttacct tttgagattg
     5221 gctatatcca cctgctacag agattgaaac aaccataaat gtagcaaata aaagtaatgc
     5281 tttgttatca gcttttccag tccatcccat tataacgaat actaatgtta atattactaa
     5341 tgaagctatt gtcatacctg atacaggaag gtttgatgtt cctattgtac cagttaaacg
     5401 tcctgcaact attacaaata gtaatgatag gattattgaa acaactgcac ctgttatagc
     5461 cattgcaata ctattagaaa taaagaatcc tataacaaaa gctaataaag ctcctactaa
     5521 aagtattatt atagatccgc tatttttttc agcattttct tcgttaacag ggcttgcttt
     5581 taatgtttct tttattgaaa ctattatagt tggtataagt tttatagccc ctataatacc
     5641 tccacaaagc atcattcctg ctcctatata cttaacataa cttcctgata ttgcatttac
     5701 atccatttgg tgaattaaca ttgaagcatc attccatgaa tgcatgctct tttctgccat
     5761 atcactgaaa tatcctatta atggagctat accaaagtta gcaagtatag atcctgcaaa
     5821 catagtaaga gatacttcta atccaactat aaacccaata ccaagtaaaa gtgggtttac
     5881 ttcagtagaa aatttccact tataaaattt gcttcctaga tatgtaacag tgttatttat
     5941 taagtttaaa aatgatcctg ttaaaacatt tataatacca cttataacaa atccaatacc
     6001 cataaattta attgagtctt cacttccttc agaagcaaca agtgtttcag atattgccat
     6061 tgactcagga tacataagtt ttccatgttc ctcaactatt agatagttat aaactagtga
     6121 tagacatcct ataccaaata gaactcctcc tacacctgca gctacacctt ctaagaaagt
     6181 aaacttactt cctattagta taattgctgg taatacaaat atcataccac ttgctataga
     6241 ttctcctcca cttgccatac cttgagttat gttttttcca agaatacctt tactacgtgc
     6301 aaatattcct acaaaaactg atcctatgat agcaccaggt ataccagcag caacagttaa
     6361 ccctgatttc atacctgaat aagcagtaga agcagcaaaa acaatagcta atactatacc
     6421 tattattagt accggaagat taactccatt ttttgactta ccagtaaaat aaggaacata
     6481 atctttacca cttacaccac cataagcttc cttaggtaat ttctttttca tacacctaat
     6541 ctctcctttc ttttattcat atcaagggat aaaaatatct attgatatgt tctattgtta
     6601 tattttctat aaaatgcaca aatattataa tatataaaaa taaagcaaaa tttataattc
     6661 tacttaaact aattattgcg catattatat ttaaactcac cttaaaagac agagtaaaaa
     6721 agctgtcaat taagttaacc tttatatagt aatttaatta tcactatact tttaaaatgt
     6781 ttttaaaact ttctaaatac ataaagaaga aatccttata attaagtatt tcattgtttc
     6841 aatgaattaa tactttgttt aaatctacct ataaaaatta gatttttctt atattttcat
     6901 ggtgtatttt actattaaaa tatttttatc atgcttataa taataccatt ttaactaaaa
     6961 aaaatccatg ataattcttt cacatataca atttttttac atttattatt aaataatctt
     7021 taaattttta aaatttaaca ttccctattc cctcttacat aaaacctata caaatactct
     7081 aagtataagc tctaaattaa ataacttcct ttaaaagtaa aaagctacac agactttatt
     7141 aatctatgta gattctcata taacatttac taatattttt cacctgttat caaattatct
     7201 tttctttctt tttactaaaa aggttcctga atccataatt actactccta attttgtatg
     7261 aatattcata atgaagctcc tctttaataa tgatgttagg tttatagtta taaactttta
     7321 taagtatatc ttttaattat gagaagactt tcttccatga ttccttactg gaacaaattc
     7381 tgaagttgta tcactcggat caatatactt ataatctcca ttttcatctt ttctaagacc
     7441 taaagatgcc atcttctttt tactatttct atttttatta cccattttta ttacctccta
     7501 taaaa
//
LOCUS       DAEVGI010000120         7469 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEVGI010000120 DAEVGI010000000
VERSION     DAEVGI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.120"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..399
                     /locus_tag="K8M77_001979"
     CDS             <1..399
                     /locus_tag="K8M77_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7086457.1"
                     /translation="DTVIITDLTRISRSTKDLLNIIDRIKAKGASIKSIKDTWLDTSS
                     DNPYNSFLLTVMSGLSQLERDLISQRTKEGLKSAKARGRNGGRPSKRNDKAHTVGLLY
                     REGYKIVDIVKQTELSRATVYRVLNDLKLK"
     gene            425..781
                     /locus_tag="K8M77_001980"
     CDS             425..781
                     /locus_tag="K8M77_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086458.1"
                     /translation="MIWSKLSSSINYYINKRIWGEELLKENILLLNQYIEDAFILEDG
                     IYKYLDKKTYEYIDLSEEDMKKIEEAFIERLEKKRKVNKDKENFKNHMIMITEYLENE
                     KIKEKSNVIELKNYRK"
     gene            complement(874..1902)
                     /locus_tag="K8M77_001981"
     CDS             complement(874..1902)
                     /locus_tag="K8M77_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7086459.1"
                     /translation="MKIAIRGGHNFLAKGACGLIDETIEDRKVYKAVIKNLIENNFEV
                     LDVTPDDCDVNTDLKFGVEKANNFNADLFVSIHFDKCYDKFDGPLGTGTWVCEKGGKA
                     EIYAQNIVDTISEGTSLKNRGVKTNAKLYELNKTIMPAVIVEVCFCESKVDVDIYREK
                     GSDLIGYLIAKGICKSVNKEISSDLPQVNLENTTNSQNNNLFKTNATAKVALDPRDNP
                     SNNYKDLGEIYANERIKILAEVCDLKFFLPATYWQDALNKESSPIWVNSKQTVLNVDT
                     NATVINVLTELDARYTPSPDSNRMGYVKNQERLFVHKIENNYALATYLASEGYKTAWF
                     TAEYIKLD"
     gene            2213..2770
                     /locus_tag="K8M77_001982"
     CDS             2213..2770
                     /locus_tag="K8M77_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBI7086460.1"
                     /translation="MSELYKNIVLCQKGDKKAIEYIINKFEILINKYKMSFLKEIHFN
                     SYDVEDNKQDLIVSLINIVNKIPIDNPQFENEGCLVNYIYRSILNSRKDMYINKNIKR
                     YFIESQSLSSMVEFKDKPLVKYIESNIEIEDMLKCLTEKEQKVIKYKFLNDKSEVEIA
                     EIMGTSRQWINRIKNTALKKLKENI"
     gene            2851..3045
                     /locus_tag="K8M77_001983"
     CDS             2851..3045
                     /locus_tag="K8M77_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBI7086461.1"
                     /translation="MDSELFKLMATQGAFAILFSYLLFYVLKENSKREDKYQNIIEEL
                     TELLPKIKDDVEDIKEKLNK"
     gene            3298..5937
                     /locus_tag="K8M77_001984"
     CDS             3298..5937
                     /locus_tag="K8M77_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7086462.1"
                     /translation="MANNIIPNVSSGDLVGSTPTFPPNAVVRGDFLYLRDVDGNQIPG
                     RTVSDGDEITVLFISNEKNIVLVQYPTSSGYRQGYVTNARSIIKYKDDYSWVNGSTPE
                     PVYDFDKTTQIGTLDPRERAVVLYKVDGMTYVAYDTGKGKLTKSGLVHYEGAGSSTGG
                     GSFNGVAPGEVVPGGFTYENNAEVVGDELYLRDANGNRIPGRSVSVGDKITVLDVSYS
                     KQLALVQYPAGNVVRQGYVTNATNLIRYFNQSMWHNGSTPEEVLDENGGHLGSLDPYE
                     SATPLYKKNGMTHVVYNTSKGPNTKSGYVKYEGSAATRVDIPRPSITNSQKIVYGTSG
                     RGRELAAYKVGNGANSLIYVCAIHGWEDHWAADGIELTRIGNGLIEHFQNAGTNNWSL
                     YIIPVANPDGLSEGYTNNGPGRCTIVGAVDCNRDFPIGFTPYGDSRHWTNKEPLSVPE
                     SRSLRDFISEIKGKTSGSTYLIDMHGWEDKTIGDSHISRAFQNQFGFVNGCYDGVYQG
                     GFLISWANSIGVRAALIELPQSCYSHSDAVNGRYLQKCINAVTNLIGGSGGSSSGGSS
                     SSDITYISNGEVINVQSFLNVRKGPGTDYDSIGQLHQGDKVSIVAKNGTWYKIKYGSG
                     YGYVHSDFVKNDTVLPITPEENDKDKEQAKNKAIEYVKNIIGTEIKLPFNTYKTIIDG
                     IVEIDFKYGLNSYIFPDAAINLNIEEGKLKANNNAGNLMLMFLDIYKKKNPLVRFLLN
                     KLIYKIEEGEISIEINPAELSIEISIASKLYENKKFEMGNKVFFSLLFKINNDEIEKI
                     KKELGEVAKEVLDELVSLINYLMENADNVFLGVLMVVLVILAAFYLVPALAASLGVTA
                     GVTALSEAIAAILVAVKAMPTLK"
     gene            6054..6968
                     /locus_tag="K8M77_001985"
     CDS             6054..6968
                     /locus_tag="K8M77_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086463.1"
                     /translation="MKTKKRLSPISIFISIILLLSLILLCFKISGKRFNTTKIEETKE
                     VETQFPYNVEFDNYKVQINKPKLFNHYVLFDYNVETKDGSPIEEGDFNITGSVKFPDK
                     AYFISSDNILNEINNNKMHIVDVGIIVARENYDLYVKNPSFLVNAREKGSSKLIYEEK
                     RIDKRIETKGIIYPTKENYKEINKTFKLDNIDFNILSLGNFDFGSFIPIFIGNISHEQ
                     SEEISNKYAIRIKCGNLNQIYNLEKWAGFYEKAIKNYSKIDSKYNKNSEYDQFYMAKI
                     LYNPNEVSRDNMEIYLINKNTNEEVKIN"
     gene            7073..>7469
                     /locus_tag="K8M77_001986"
     CDS             7073..>7469
                     /locus_tag="K8M77_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7086464.1"
                     /translation="MEYALKLNKINYIDNVHSNSKGWITRSVIDKKGYSQWHYKYAEL
                     KDLDMSGENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTCKTKDQVLMDLEKNYF
                     NQSIPIPNYVIDSGRGMYLIWIINAVPSKA"
BASE COUNT         3078 a          756 c         1239 g         2396 t
ORIGIN      
        1 gatacagtta taattactga tttgaccaga atatcaagaa gtacaaagga tttacttaat
       61 attatagata gaataaaagc taaaggagct agtataaaaa gtattaaaga tacttggcta
      121 gatacatcaa gtgataatcc atataatagt tttttactaa ctgttatgag tggattgagt
      181 caattagaga gggacttaat aagtcaaagg actaaagaag gtttaaaaag cgcaaaagct
      241 aggggaagga atggaggaag gccttccaag agaaatgata aagcccatac agtagggtta
      301 ctatataggg aaggttataa aatagttgat atagtaaaac aaacggaatt aagtagagca
      361 actgtatata gagtgctaaa tgatttaaaa ttaaaataaa aaatatatta aaggggatga
      421 gagtatgata tggagcaagt taagctcaag tataaattat tatattaata agagaatatg
      481 gggagaagaa cttttaaaag aaaatatttt attattaaat caatatattg aggatgcgtt
      541 catacttgag gatggtattt ataaatattt agataaaaaa acatatgagt atattgatct
      601 aagtgaagaa gatatgaaaa aaatagagga agcttttatt gagagattag agaaaaaaag
      661 aaaagtaaat aaagacaaag aaaattttaa aaatcatatg ataatgatta ctgagtattt
      721 agaaaatgaa aaaattaaag agaaaagtaa tgttatagaa ttaaaaaatt atagaaaata
      781 atgtgttatt ttaaaagaat attagttaat aaaaaaagac taaaaagtaa tataactttt
      841 ttagtctttt tctattatat ttattaaaga ttattaatct aattttatat actctgctgt
      901 aaaccatgct gttttatatc cttcactagc taagtaagta gctaaagcat aattattttc
      961 aattttatga acaaaaagtc tttcttgatt ttttacatat cccattctat ttgaatcagg
     1021 agaaggagta tatctagcat ctaattcggt taaaacattg attacagttg cattggtatc
     1081 aacattaagt actgtctgtt ttgaatttac ccatatagga gaagattctt tatttaatgc
     1141 atcttgccaa tatgttgctg gtaagaaaaa ttttaaatca caaacttcag ctaaaatttt
     1201 tattctttca tttgcataga tttcacctaa atctttgtag ttattactag ggttatccct
     1261 aggatctaaa gcaacctttg ctgttgcatt agttttaaat aaattattat tttgtgaatt
     1321 agtagtgttc tcaagattaa cttgaggcaa atcagaacta atttctttat ttacagattt
     1381 acaaattcct ttagctatta aataaccaat taaatcagac cctttttctc tataaatatc
     1441 tacatctact ttagattcgc agaaacaaac ttcaactatt acagctggca tgattgtttt
     1501 atttaattca taaagcttag cattagtttt aactccccta ttttttaagg aagttccttc
     1561 agagattgta tctactatat tttgagcata tatttctgct tttccccctt tttcacaaac
     1621 ccaagttcct gtacctaaag gaccatcaaa tttgtcataa catttatcaa agtgtataga
     1681 aacgaataaa tctgcattaa aattattagc tttttctact ccaaatttta agtctgtatt
     1741 tacatcacag tcatctggag taacatctaa aacttcaaaa ttattttcaa ttaagttttt
     1801 aataacagct ttataaactt ttctatcttc tatagtttca tctattaacc cacaagcacc
     1861 ttttgctaaa aagttatgtc caccacgtat agctattttc atattatcac ctttctttct
     1921 taaattttta tgttcttata tattaaaaag tgttaggatt tgattttgta aacataaaaa
     1981 ttaaagaaag ttagattttt ataaaaaata gatatttaca aaatagacta aaaaaagctt
     2041 tttatatagt ataagctttt agtctatttt tatattgttt gggtttattg acttatttgt
     2101 gaaaaagttg taaaattaat acgaacatat gttcgtatta aaaatagaac attttattaa
     2161 aaaaaaaaac actctaccat gcaaaaaata aattaatatg ggtagagtgc gtatgagtga
     2221 attatataaa aatatagtat tatgtcaaaa aggagataaa aaggctatag aatatataat
     2281 aaataagttt gaaatattaa ttaataaata taaaatgtct tttcttaaag agatccattt
     2341 taatagttat gatgttgaag ataataaaca agatttaata gttagtttaa taaatatagt
     2401 taataaaata cctattgata atcctcaatt tgaaaatgaa gggtgtttag ttaattatat
     2461 ttatagatca atattaaaca gtagaaagga catgtatata aataaaaata taaaaagata
     2521 ttttattgaa agtcagtctt tatcaagcat ggtagagttt aaagataagc ctttggtaaa
     2581 atatatagaa tctaatattg aaattgaaga tatgctaaaa tgccttacag aaaaagaaca
     2641 aaaggttata aaatataaat ttttaaatga taaaagtgag gtagagatag ctgaaataat
     2701 gggaacatca agacaatgga taaatagaat aaagaataca gctttaaaaa aattaaaaga
     2761 aaatatttag ttattaattt aatctatttt tattaaagac aaaaattagg cttgaattgt
     2821 taaataaaaa tggattggag gttagctaaa atggatagtg aattatttaa gttaatggca
     2881 acacaaggag cctttgcaat attattttcg tatttattgt tttatgtttt gaaggaaaat
     2941 agtaaaagag aagataagta tcaaaatata atagaggagc ttacagaatt attgccaaaa
     3001 ataaaagatg atgtagaaga tataaaagaa aaacttaata aataggatga gaagtcgata
     3061 tttaaaaaat atcggctttt ttaattttta ggtttacatt tttaaaacta aactcttttt
     3121 atttataaag agtaatttaa atttcaaaaa aataaaaaaa ttataaattt agtttacaaa
     3181 attgaagtca aattactctt tatattatgt aaaacaaatt caagcttgat ttttgtaaaa
     3241 agaaaaattt aaaagattaa attcaaataa aaaataaaga aaagagaggt tttaaaaatg
     3301 gcaaacaata taattccaaa tgttagttca ggggatttag taggaagtac acctacgttt
     3361 ccgccaaatg cagtagtaag aggagatttt ttatatctaa gagatgttga tggtaatcaa
     3421 attccaggaa gaacagtaag tgatggagat gaaattacag ttttatttat aagtaatgaa
     3481 aaaaatatag tattagttca atatccaaca agttctggat atagacaagg atatgtaaca
     3541 aatgcaagaa gcataataaa atacaaagac gattattcat gggtaaatgg ttcaacacct
     3601 gagccagtat atgactttga taaaacaaca caaataggaa cattagatcc aagagaaaga
     3661 gccgtagttc tttataaagt agatggaatg acatatgttg cttatgatac aggaaaaggt
     3721 aagcttacaa agtcaggatt agttcattat gaaggagctg gtagttctac tggaggcgga
     3781 agttttaatg gagtagcacc aggagaggtt gtaccaggtg gattcacata tgaaaacaat
     3841 gcagaggtag ttggtgatga actatatcta agagatgcaa atggtaatag aataccaggt
     3901 agaagtgtaa gtgtaggaga taaaataact gttttagatg ttagttatag caagcaatta
     3961 gctttagtac agtatccagc tggcaatgta gtaagacagg gttatgtaac aaatgcaact
     4021 aacttaataa ggtactttaa tcaaagtatg tggcacaatg gaagtacacc agaggaagta
     4081 ttagatgaaa atggtggaca tttaggatca ttagatccat atgaatcagc aacaccacta
     4141 tataagaaaa atggaatgac acatgttgtt tataatacat ccaaaggacc taacacaaag
     4201 tcaggttatg taaaatatga aggttcagca gctactagag tggatatacc tagaccatca
     4261 ataacaaatt cacaaaaaat agtatatgga acaagtggaa gaggaagaga attagcagct
     4321 tataaagttg gtaatggagc taattcattg atatatgtat gtgcaataca cggatgggaa
     4381 gatcactggg cagcagatgg tattgagtta actagaatag gtaatggact tatagagcac
     4441 ttccaaaatg caggaacaaa caattggtca ctatatataa ttccagtagc aaatccagat
     4501 ggattatcag aaggatatac aaataatggg ccaggaagat gtactatagt aggtgcagta
     4561 gattgcaata gagatttccc aataggattt acaccatatg gagattcaag acattggaca
     4621 aataaagagc cgttaagtgt accagaatca agaagtttac gtgactttat aagtgaaata
     4681 aaaggaaaga catcaggttc aacatattta atagatatgc acggatggga agataagaca
     4741 ataggagatt cacatatatc aagagcattc caaaatcaat ttggatttgt aaatggatgt
     4801 tatgatggag tatatcaagg aggattttta atatcatggg caaattcaat aggagtaaga
     4861 gcagccttaa tagaattacc acaatcatgt tatagtcatt cagatgctgt taatggaaga
     4921 tatcttcaaa agtgtataaa tgcagtaaca aatctaatag ggggttctgg cggtagttca
     4981 tcaggaggaa gttcatcttc agatataact tatatatcaa acggagaagt aataaatgta
     5041 caatccttcc taaatgtaag aaaaggacca ggaacagatt atgactcaat aggacagttg
     5101 catcaaggag ataaggtaag catagtagct aaaaatggaa catggtataa aataaaatat
     5161 ggttcaggct atggttatgt acattctgat ttcgttaaaa atgatacagt gttgcctatc
     5221 acgccagaag agaatgataa ggataaagaa caagctaaga ataaagctat agaatatgtt
     5281 aaaaatataa ttggaactga aattaagtta ccatttaaca catataaaac aataatagat
     5341 ggaattgtgg aaattgattt taaatatgga ttaaatagtt atatatttcc tgatgctgca
     5401 ataaatctta atattgaaga gggaaagtta aaagctaata ataatgcagg aaatttaatg
     5461 ttgatgttcc ttgacattta taaaaagaaa aatccattag ttagattttt actaaataaa
     5521 cttatatata aaattgaaga aggagaaatt tcaatagaaa ttaatccggc tgagctatct
     5581 atagaaatat ctatagcatc aaagttatat gaaaacaaaa agtttgagat gggaaataag
     5641 gttttcttta gcttattgtt taaaattaat aatgatgaga tagaaaagat taaaaaagaa
     5701 cttggagaag tcgcaaaaga agtattggac gaattagtaa gtttaataaa ttatttaatg
     5761 gaaaatgcag ataatgtatt tttaggtgtt cttatggttg tattggtaat tttagcagca
     5821 ttttatttag taccagcatt agcagcttct ttaggagtaa cagcaggagt aacagcttta
     5881 tcagaggcaa ttgcagcgat acttgtagca gttaaagcaa tgccaacatt aaaatagtaa
     5941 tattatttaa gagttatata tcatagaatc catctatgac atataacttt tatgctataa
     6001 ttattaaaaa tattttaaat tataaaatac aatatatttg aaaggaatag cgaatgaaaa
     6061 caaaaaaaag attatctcca attagtattt ttataagtat aattttattg ttaagtctta
     6121 tacttctgtg ttttaaaata tcgggtaaga gatttaatac cacaaaaata gaggaaacta
     6181 aagaagtgga aacacaattt ccgtataatg tagaatttga taactataaa gttcaaataa
     6241 ataagccgaa attattcaat cattatgttc tttttgatta taatgttgag acaaaggatg
     6301 gttcacctat agaagaagga gattttaata ttacaggtag tgttaaattt cctgataaag
     6361 cttattttat atcttctgat aacatattaa atgaaattaa taacaataaa atgcatattg
     6421 ttgatgtcgg gattattgta gctagagaaa attatgattt atacgtaaaa aatcctagtt
     6481 ttttagtaaa tgcaagagaa aaaggtagta gcaaattgat ttatgaagaa aaaagaatag
     6541 ataaaaggat agaaactaaa ggaattattt atccaactaa agaaaactat aaagaaataa
     6601 ataaaacatt taaattagat aatattgatt ttaatatttt aagtttagga aattttgatt
     6661 ttggatcatt tatacctatt tttataggta atataagtca tgaacaaagt gaagaaataa
     6721 gtaataaata tgctattaga attaaatgtg gaaatttaaa tcaaatttat aatttagaaa
     6781 aatgggctgg attttatgaa aaagccataa aaaattatag taagatagat tctaaatata
     6841 ataaaaattc agaatatgat caattctata tggcaaaaat tttatataat cctaatgagg
     6901 taagtagaga taatatggaa atatatctta ttaataaaaa tactaatgaa gaagttaaaa
     6961 taaactaaaa ttttcatgag attagagatt tgcaataaat tttattgcaa atttttttat
     7021 aaatatattt gtgatataat tttcttagtt ttacaaaagg ggaggtgaaa aagtggaata
     7081 cgcattaaaa ctgaataaga taaactatat agataatgtt catagtaaca gtaaagggtg
     7141 gataaccagg agcgttatag ataaaaaagg atatagtcaa tggcactata aatacgctga
     7201 attgaaagat ttagatatga gtggtgaaaa catttatata actctaaata cgttctataa
     7261 gccttgtaga aggctagaaa atataaagga attgaatact ttatttatag acttagatta
     7321 ttataaaact tgcaaaacta aggatcaggt attaatggac ttagaaaaga attattttaa
     7381 tcaaagtatt cctataccaa actatgtaat agatagtggg agaggaatgt atttaatatg
     7441 gataataaat gcagtaccta gtaaagcat
//
LOCUS       DAEVGI010000121         7466 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEVGI010000121 DAEVGI010000000
VERSION     DAEVGI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.121"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(93..1406)
                     /locus_tag="K8M77_001987"
     CDS             complement(93..1406)
                     /locus_tag="K8M77_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBI7086465.1"
                     /translation="MKKGKFSALLPLIIFVSIYLGTSLVMKDFYSVSVLVPGIIAVLF
                     GIFTNRKRGLEKNIEIFCKGAGNSNIILMCLIFILAGAFAEVAKNMGAVESTVNLGLT
                     ILPKNILVAGIFIISSFIAISLGTSMGTIAAIVPIAMGISDKTNIALPLIVGAVVGGA
                     MFGDNLSMISDTTIAATKTQGCEMKDKFKINFKVILPAAILVIVIYTFLGSGANTSVG
                     QYDYNFIKIIPYLGILIAALIGANVIAILSGGIVLAGAIGFFTGSLDLKTFFTSISTG
                     IGGMSELILISIIIGGIVEIIKENGGIDYVLNLVTKRINGKKGAEFGIGLLVSLVDAC
                     TANNTIAIVTVGPLAKDISDEYNLDSKRIAGILDMFSCAVQGIIPYGAQLMSAAALTG
                     LSSFEIMKFLFYPYLMGISVIAFIAFFQKKESAEKSGELNAIAVE"
     regulatory      complement(1506..1669)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1744..2586)
                     /locus_tag="K8M77_001988"
     CDS             complement(1744..2586)
                     /locus_tag="K8M77_001988"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HBI7086466.1"
                     /translation="MKPMNKIAAIHDISCYGRAALATIIPILSSMGNQVCPLPTAVLS
                     THTDGFGKPAIRDLSDFIYETKDHWKRLNLNFQCIYSGYLADPNQVKFVEKFIEEFKE
                     DDTLVVIDPVMADNGKLYDGMSEKMIEEMRTLIKSADIITPNITEASFLLGKEYKESL
                     NEDEIKEYLVGLGDLGPKISIITSATSLKGNEYIDTVLYDREEKMFYTYSHKRINAFY
                     CGTGDAFASLLIGWILKGESIEKALEMSCNFIAEAIEYSEKFDYPPNEGILLENLLYK
                     LISK"
     gene            complement(2723..2798)
                     /locus_tag="K8M77_001989"
     tRNA            complement(2723..2798)
                     /locus_tag="K8M77_001989"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2763..2765),aa:Lys,seq:ctt)
     gene            complement(2817..2890)
                     /locus_tag="K8M77_001990"
     tRNA            complement(2817..2890)
                     /locus_tag="K8M77_001990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2856..2858),aa:Gly,seq:tcc)
     gene            complement(2895..2970)
                     /locus_tag="K8M77_001991"
     tRNA            complement(2895..2970)
                     /locus_tag="K8M77_001991"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2935..2937),aa:Pro,seq:tgg)
     gene            3137..3427
                     /locus_tag="K8M77_001992"
     CDS             3137..3427
                     /locus_tag="K8M77_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086467.1"
                     /translation="MKISSIVMLAASFLLIVVGIVLFANRKRFEGENQAGKYNAKYIQ
                     SNAIGNIFIGFLGTILGVLDNFVNGNSIKIAFAVIIIGGSIIQKLIGNKISK"
     gene            3610..4908
                     /locus_tag="K8M77_001993"
     CDS             3610..4908
                     /locus_tag="K8M77_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBI7086468.1"
                     /translation="MKKKIISTVLAATMTFGVGHSVFATPLTDDQKQQMEHNQDKYAD
                     INSKIRELEDKIDGLSAKIEPLFFQVEKNKEEISKTEESISTVKVQIEESKKKIEKQQ
                     EVLGQRIRATYKSGGQANYLTALLDSNGIGDFLSRVQAISKVMGMDKQVIDELTSEKE
                     KLDSEVKELQDKTTELNKLNSETQSKIDELNKMKAEQEGVIKDMKAEQEKVVGELAPL
                     ERQLMEPWTSKINSNSSLNDLNQAVTALRGLRNQIKTPEVDAEAVQAIEKAKDLIETK
                     KAEEAVSSAPNRGGDVNTGGSSSSASNGSSSSNSGSTVAPPSEGAASAVVSYAYQFIG
                     RPYVFGATGPDTFDCSGFTSYVYRNAVGREITRTTYTQINQGRPVSRDQLQPGDLVFT
                     NGVEHVGIYVGGGQMIHAARPGVGVIVGPIYNFSSARRIL"
     gene            5082..5852
                     /locus_tag="K8M77_001994"
     CDS             5082..5852
                     /locus_tag="K8M77_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBI7086469.1"
                     /translation="MNKALKELDLTLVKENETLDDLQLDGLHLIQKKEGFRFGVDAVL
                     LANFANVNRKHSVLDLCTGTGIIPFIIYGKKNPKEVVGIEIQEEFAEMANRSAKINNI
                     EDKVSFVCGDLKDKELNRSISKFDVVTVNPPYKLNNAGIVNPEDKLAIARHEIMCNLE
                     DVIAASRRFLKDNGRLYMVHRPERLADIFCLMRKYKIEPKRVRMVHPNTKKAANIVLV
                     EGQRDGGAFLKWEAPLYVYKDEGGYSEEIDKIYGRESK"
     gene            5855..6697
                     /gene="rsmI"
                     /locus_tag="K8M77_001995"
     CDS             5855..6697
                     /gene="rsmI"
                     /locus_tag="K8M77_001995"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBI7086470.1"
                     /translation="MSKVYLVPTPIGNLGDITLRALEVLKNVDLIAAEDTRQTLKLLN
                     HFEIKKPLISYHKHNEQGKGEEILRRVKEGTSVAIVSDAGTPGISDPGSVIVEKCIEE
                     GIEFEVLPGATAFTTALIYSGLDTTKFMFKGFFPRENKDKRSFIEDIKDRTETIIIYE
                     SPYRILDTIDTLKEGLGNRKVAICRELTKLHEEIFRGTLEEAKIHFEENAPKGEFVCV
                     ISGKTDKEIEEENTSKWISMSIEEHIIHYIDNEGMKKKDAIKQVAKDRGVAKSEIYKF
                     SLDI"
     gene            complement(6864..7103)
                     /locus_tag="K8M77_001996"
     CDS             complement(6864..7103)
                     /locus_tag="K8M77_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBI7086471.1"
                     /translation="MKSTGVVRRVDELGRIVIPIELRRTLDIAEKDALEIYVDGEQII
                     LKKYEPACIFCGDARDVVNYKGKNICQKCLDELKK"
BASE COUNT         2787 a         1038 c         1134 g         2507 t
ORIGIN      
        1 tttattaata agttattttg atacacgcct tttttttatt ttatactaaa atttatcaaa
       61 ccccttttta atgcttattg ctttttaata ttttattcaa ctgctatagc atttaactcc
      121 ccactttttt cagcactttc tttcttttgg aaaaatgcta tgaatgctat tacacttatt
      181 cccattaaat atggatagaa taaaaatttc attatttcaa atgaacttaa tccagttaaa
      241 gccgccgctg acattaattg tgctccatat ggaattattc cctgaactgc acaagagaac
      301 atatctaata ttcctgcaat tctttttgaa tctaagttat attcatctga aatatccttt
      361 gctaaaggtc caactgtaac tatagcaata gtgttatttg cagtacatgc atcaactaag
      421 cttactaata atccaatacc aaattcagcc ccttttttgc cattgattct cttagtaact
      481 aagtttaata catagtctat accaccattt tcttttatta tttcaacaat tcccccaatt
      541 attattgaaa ttaatataag ttcactcatt ccccctatac cagttgatat tgaagtaaag
      601 aaagtcttta aatctaaaga tcctgtgaag aatccaattg ctccagctaa tactattcca
      661 cctgataata tagcaattac attagcccca attaatgcag ctattaaaat acctaagtaa
      721 ggaataatct taataaaatt ataatcatat tgccctactg atgtattagc cccacttcct
      781 aagaatgtat atattacaat tactaaaata gcagctggta atataacctt aaagtttatt
      841 ttaaacttat ccttcatttc acatccttga gtttttgttg ctgctattgt tgtatcagaa
      901 atcattgata gattgtcccc aaacatagct ccacctacta ctgccccaac tataagtggt
      961 aatgctatat tagtcttatc tgaaattccc attgctattg gaactatagc agctattgtt
     1021 cccattgatg ttcctaaaga tatagctata aacgatgata ttataaatat tcctgctact
     1081 aatatatttt taggtaatat tgttagccct aaatttactg tagattctac agctcccata
     1141 tttttagcaa cctctgcaaa ggcaccagct aatataaaga ttaagcacat taaaattata
     1201 ttactattac cagccccctt acaaaatatc tctatgttct tttcaagtcc tctttttcta
     1261 tttgtaaata ttccaaataa aactgcaatt attcctggaa ctaaaacaga tacagagtag
     1321 aaatctttca ttactaatga agttcccaaa taaatcgata caaaaattat taatggtaat
     1381 aatgctgaaa attttccttt tttcattttc tctcctccta atccatttaa taaatctatc
     1441 ctttatccta caaaataaaa aaacttctat cctaggatag aagttattat tcttacagat
     1501 tctctttctt atctctcagt ttctaccgca ggatttagca ccgctataaa tctttgcata
     1561 ggaaattact actgattttt tcattttaaa atcagcattg ttttaaccgc aaagaattac
     1621 cggttgctgg gtttcacagg gccagtccct ccacctctct tgataagatt tattaaattt
     1681 tgtttttttt aatgttatca ttctaaaatt actttgtcaa tttatttttt ttgctttaat
     1741 aatttattta cttattaatt tatataacaa attttcaagt aatattcctt catttggagg
     1801 ataatcaaac ttttcgctat attctatagc ttctgcaata aaattacatg acatctccaa
     1861 tgctttttca atactttccc cttttaatat ccatcctatt agtaatgaag caaaagcatc
     1921 gcctgttcca caataaaaag catttattct tttatgagaa taggtataaa acattttttc
     1981 ttctctatcg tatagtacag tatcaatata ttcatttcct tttaaacttg ttgcacttgt
     2041 tattatactt attttaggtc ctaaatctcc taaacctact aaatactctt ttatctcatc
     2101 ttcatttaaa gattctttat attcttttcc taaaagaaaa gaagcttctg taatatttgg
     2161 agtgattata tctgcacttt ttattaaagt tctcatttct tcaatcattt tttcactcat
     2221 accatcataa agcttaccat tatcagccat aacagggtct ataactacta gagtatcatc
     2281 ttctttaaac tcttctataa atttttcaac aaatttaact tgattaggat cagctaaata
     2341 tccactatat atgcattgaa aatttaaatt aagtcttttc caatgatctt tggtttcata
     2401 tataaaatcg cttaaatctc taattgctgg ttttccaaat ccatctgtat gtgttgacaa
     2461 tactgctgtt ggaagtggac atacttgatt tcccattgaa gataatatag gtattattgt
     2521 tgctaatgct gccctcccat agcatgaaat atcatgaata gcagcaatct tattcatagg
     2581 tttcatttct atctcccctt aaaaatctac ttttaaataa aattcttatt atctagtatt
     2641 atattaattt aattttttta tatcaaatgt acccatactt ttatataaaa taaaaaaaaa
     2701 gcatcgttta taaaacgatg cttggtgcac catcagggat tcgaaccctg gactccctga
     2761 ttaagagtca ggtgctctac caactgagct aatagtgcat attatatttt ttaaaatgga
     2821 gcgggtaccg ggaatcgaac ccgggtatcc agcttggaag gctggtgttc taccattgaa
     2881 ctacacccgc ataatggtcg gggtgacagg gatcgaacct gcgacctcat ggtcccaaac
     2941 cacgcgcgct cccatctgcg ccacaccccg gcgacattta ttattatatg agaaaataat
     3001 aaggttgtca acaaaaaaat aaaaaaaagt ttaagaaatt aaaaaatatt atattctact
     3061 taaaatatat tatatttctg tgaggatgtt ataatgttaa gtagtaattt aaaaattaaa
     3121 agatgaggtg aaacaaatga agataagttc aattgttatg ctagcagcta gttttttatt
     3181 aatagttgtt ggaatagtat tatttgcaaa cagaaaaaga tttgaaggag aaaatcaagc
     3241 gggaaaatat aatgctaagt acattcaaag taatgcaata ggaaatattt ttataggatt
     3301 tttaggaact attttaggtg tacttgataa ttttgttaat ggaaattcaa taaaaatagc
     3361 tttcgcagtt attataatag gtggatcaat tattcagaaa ttaattggaa ataaaatctc
     3421 aaaatagtat atatatgtaa gaaaaagtta aaaagtgtaa aaaagtgaaa aaaatatatt
     3481 tgtttaacaa aaaaatgaca aatttatatt gttacaaaaa ggtttcaaat gctataatgt
     3541 ataagtataa aacttttaac ataggggagg gatgagttcc tatttttcat taaatgggag
     3601 caagtagata tgaagaaaaa aataatttca acagttcttg ctgctacaat gacatttgga
     3661 gtaggacaca gtgtattcgc aactccactt acagatgatc aaaagcaaca aatggaacat
     3721 aatcaagata aatatgcaga tattaatagt aagataagag agttagaaga taagatagat
     3781 ggattatcag ctaaaataga acctttattt ttccaagtag aaaaaaataa agaagaaata
     3841 tctaagacag aggaatcaat ttcaacagtt aaagttcaaa ttgaggaatc aaagaaaaaa
     3901 atagaaaaac aacaagaagt tttaggtcaa agaataagag caacatataa aagtggtgga
     3961 caagcaaact acttaactgc tttattagat tctaatggaa ttggtgactt cttatcaaga
     4021 gtacaagcta taagcaaagt aatgggtatg gataagcaag ttattgatga attaacttct
     4081 gaaaaggaaa aacttgatag tgaagttaag gaattacaag ataagactac agaattaaat
     4141 aagcttaatt cagagactca atcaaaaata gatgagttaa acaaaatgaa agctgagcaa
     4201 gaaggcgtta taaaagacat gaaagctgag caagaaaaag ttgttggaga attagcacca
     4261 ctagaaagac agttaatgga gccttggact tctaaaataa attcaaatag ttcattaaat
     4321 gatttaaatc aggctgtgac agccttaaga ggtttaagaa atcaaataaa aactccagag
     4381 gtagatgcag aagctgttca agctatagaa aaagctaaag atttaataga aactaaaaaa
     4441 gcagaggaag cagtaagcag tgcacctaac agaggtggag atgttaatac tggtggaagc
     4501 tcatcttcag ctagtaatgg aagttcatca tcaaatagtg gaagcacagt agctccacca
     4561 agtgaagggg cggcttcagc agtagtatca tatgcatacc aattcatagg aagaccatat
     4621 gtatttggtg caactggtcc tgatacattt gattgttcag gatttactag ctatgtatat
     4681 agaaatgcag taggtagaga aattactaga actacttata cacaaataaa tcaaggtaga
     4741 cctgtatcaa gagatcaatt acaaccagga gatttagtat ttactaatgg tgtagaacac
     4801 gttggaatat atgtaggtgg tggacaaatg attcacgctg ctagaccagg tgttggtgta
     4861 atagtaggcc ctatatataa tttctcatca gcgagaagaa tattataatt taaaatatat
     4921 ttatgccaaa cagtagaaat aactgtttgg catatttttt tataaaaggt taagaatttt
     4981 gagtatatgc tataatatag actataacaa ttaaagagac cttgatttat ttactgattt
     5041 tacaggtcta ctgattacaa taaaggtgag tgtgaagaag tatgaataaa gctttaaaag
     5101 agttggattt aactttagtt aaagagaatg aaactcttga tgatctacag ttagatggat
     5161 tacatctaat tcaaaaaaaa gaagggttta gatttggagt ggatgctgtt ttgttagcta
     5221 attttgctaa tgttaacaga aaacattctg tattagattt gtgcacaggt acaggaataa
     5281 ttccttttat tatttacgga aaaaaaaatc ctaaagaagt agttggaatt gagatacaag
     5341 aagaatttgc tgaaatggca aatagaagtg caaagattaa taatattgaa gataaagtat
     5401 cttttgtatg tggagattta aaggataaag agcttaatag atcaatatct aaatttgatg
     5461 ttgttacagt aaatcctcca tataagttaa ataatgcagg tattgtaaat ccagaagata
     5521 agcttgctat agctagacat gagattatgt gtaatttaga ggatgttata gcagcaagta
     5581 gaagatttct aaaggataat ggaagacttt atatggtaca tagacctgaa aggttagctg
     5641 atatattttg tcttatgaga aaatataaaa tagaacctaa aagagttaga atggttcatc
     5701 caaatacaaa aaaagctgca aacatagttt tagtagaagg tcaaagagac ggcggagctt
     5761 tcttaaaatg ggaagcacct ttatatgtgt ataaagatga aggtggatat agtgaagaaa
     5821 tagataagat atatggaagg gagagtaaat aaaaatgagt aaagtttatt tagtaccgac
     5881 tccaatagga aatttaggag atataacttt aagagcctta gaggttttaa aaaatgttga
     5941 tttaatagct gctgaggata caagacaaac acttaagctt ttaaatcatt ttgagattaa
     6001 aaagccttta attagttatc ataagcataa tgagcaaggt aaaggtgaag aaatacttag
     6061 aagagttaaa gaaggaactt cagttgctat agttagtgat gctggaactc caggaatatc
     6121 agatccagga agtgtaattg ttgagaaatg tatagaagaa ggtatagaat ttgaagtttt
     6181 accaggagca acagctttta caactgcttt aatatattct ggattagata caactaaatt
     6241 tatgtttaaa ggatttttcc caagagaaaa taaggataaa agatctttta ttgaagacat
     6301 aaaggataga acagaaacaa taataatata tgaatcacca tatagaatat tagatactat
     6361 agatacttta aaagaagggt taggaaatag aaaagtagct atatgtagag aattaactaa
     6421 gcttcatgaa gaaatattta gaggaacttt agaggaagca aaaattcatt ttgaagaaaa
     6481 tgctccaaaa ggtgaatttg tttgtgtaat atcaggaaaa acagataaag aaatagaaga
     6541 agaaaatact tcaaaatgga ttagtatgtc tatagaggaa catataatac attatataga
     6601 taatgaagga atgaagaaaa aagatgctat caaacaagta gcaaaggata gaggagttgc
     6661 aaaatcagaa atatataaat tttcgttaga tatttagggt taacatataa gatttaagta
     6721 ttatatattt aatttaataa aaaataaagc acttcatagg ggattatgaa gtgctttaac
     6781 actataaggg gatatagtgt taaaaattat tattggttag ggttaaaatg ttaattaatt
     6841 atttatatta aataagttaa ttactatttt tttaattcat ctaaacattt ttggcagatg
     6901 tttttacctt tgtagttaac aacatctcta gcatctccgc agaatataca tgctggttca
     6961 tattttttta atatgatttg ctctccatct acatagattt ctaaagcatc cttttctgcg
     7021 atatctaatg ttcttcttaa ctctatagga ataactattc ttcctaattc atctactctt
     7081 cttactacac ctgttgattt cattatattt cctccttgca ttttacaact ttcgacaaaa
     7141 cgcattataa tattaacaaa cttgtcatat taagtcaata tgttttgtca aaaaaaatgt
     7201 ttgtttacaa ttttttttat tctcaatcaa ttatatatta aatatactac catttttttg
     7261 aaaagtcaag gtaaagatta atttatttta cataaataga ctaatttttg tttgacaaaa
     7321 agttaaaaaa aaggataaaa attgatatat ttatataaat ttatgcctat aaaataacag
     7381 ttaacttatt aaatatatat ttaaaaataa aattggaaaa aaataataaa aatgtcgtat
     7441 agaaaattat ttgatttata taatat
//
LOCUS       DAEVGI010000122         7375 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEVGI010000122 DAEVGI010000000
VERSION     DAEVGI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7375
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.122"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(133..636)
                     /locus_tag="K8M77_001997"
     CDS             complement(133..636)
                     /locus_tag="K8M77_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7086472.1"
                     /translation="MEDLKIRRFEENEGGEVSKLIEKNFLDTNIKGYSKEDMRNLASN
                     YTENKILNMAKNSHMYVAILKNKIVGCGAIASFLGEEDESILLPIFVLPEFQGKGIGK
                     KIMETLERDEYYIRARRVEVKSSTMACDFYKKMGFNHKYNLKELDKNGNYKLEKFRYR
                     LQYDQNY"
     gene            1027..1305
                     /locus_tag="K8M77_001998"
                     /pseudo
     CDS             1027..1305
                     /locus_tag="K8M77_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
     gene            1364..1828
                     /locus_tag="K8M77_001999"
     CDS             1364..1828
                     /locus_tag="K8M77_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086473.1"
                     /translation="MAFIILGIYILINIVLLHGTKKARKGKKTVTIKIMSLISIIMFI
                     LSLLILLFGFMSYLHHKNMLNLIISIIYLFVLIFISYKLSCFYITEDTIIYGYNCYNF
                     KDIQKIEIEKVKNKFNITIYIKGSNTLYFKLDYLKKKKLLNALHGKVLCINL"
     gene            complement(1885..2910)
                     /locus_tag="K8M77_002000"
     CDS             complement(1885..2910)
                     /locus_tag="K8M77_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBI7086474.1"
                     /translation="MDNMKKILKKMPKVMKKSFKKSNTHDVLLEQANRMVINDNNLYL
                     NNKWFSKSLKWCMDILELNYNVSKSLGWIRPSYSRKPDIMLRSVLTLFSFHFYHGEDK
                     DNYFGRIGKGKTAMCKFIFFGRKALKYRDKGKRRKCMKYLSYSCHYLADMSEPHHVTH
                     HIASPNERIMNVLKKFDINKIGKGNFSNHRRFENLAQAWIKGDEDITLLLDKNKDKDE
                     NILLKGVPTKGEIFSYILKDEGIKVFNEDDKKKVLDIYYKNSNLDFDDYCRYLGNESS
                     EYAKELIHYAFKDDTREQALAAIKALNMAEIQLARFLYYFCNYNLKEEKFVEPKLKLY
                     NLSLGVS"
     gene            complement(3025..4203)
                     /locus_tag="K8M77_002001"
     CDS             complement(3025..4203)
                     /locus_tag="K8M77_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI7086475.1"
                     /translation="MILENMILDNVKNMPPSGIRKYFDLINEMEDVISLGVGEPDFVT
                     PWNVREAGIYSLEQGHTHYSSNAGFIELREEISKYLNRRFSLRYNPKDEILVTVGGSE
                     GIDLALRALVGPGDEVIIPEPSFVAYKGCTAFTGATAKTIDLRACDDFKLTPELLEEA
                     ITEKTKVVIIPFPNNPTGAIMNKEELQKIVDVLKDKDVIIISDEIYAELSYDEDHVSI
                     ASFPEVKEKTIVINGFSKAYAMTGWRLGYVCAHKVLIDAMKKIHQYAIMCSPTTAQYA
                     AIEALKNGDESVFEMAREYNRRRRVLVDGFRSMGLDCFEPLGALYVFPCIKSTGMSSD
                     EFCEKLLLEEKVLAVPGNAFGECGEGFIRACYAASMEDIMEAIKRIRRFVERNNMQGK
                     "
     gene            complement(4200..4682)
                     /locus_tag="K8M77_002002"
     CDS             complement(4200..4682)
                     /locus_tag="K8M77_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBI7086476.1"
                     /translation="MEEILEVLEKNSRYTDQEIAVMTGKTVEEVREAIRDYEERSIIA
                     GYTTLINWENTGRETVTALIEVQITPQRGVGFDKVAERIYKFPQVKACYLMSSGGFDL
                     TVIVEGKTMKEVAMFVSDKLSIQEYVTGTATHFVLKKYKDHGTIFREKKTDDREMLFI
                     "
     misc_binding    complement(4775..5116)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5212..6423)
                     /locus_tag="K8M77_002003"
     CDS             complement(5212..6423)
                     /locus_tag="K8M77_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086477.1"
                     /translation="MKKFWSIFTFTYKETIRNKALIISTIITLIVMLGFFNMDKIVGL
                     FNREGSKDLIMISTEANNKFFDSNTLESLNDDDFKVVLSKDSEETNKVKKEIENGKSD
                     YKALLEVSTGKEVSGKLYVNELPGDSLMKKVNTILNDTKFVFSMEKFNITPEEYANIM
                     TPAKLDVVQTGNASHERMALVYALVFGIYIITLTFGSMVANSVIEEKSNRIMETLITM
                     AKPMELFFGKVLGICAVGLTQIAVILGSGLIMLKTSGASLEVLSSLNLNASIIFAFVA
                     YFLLGYLTFSMLYAAVASLATSSQDVNSSMGAITLVFVGVFLLAMNCMFNIESTLTKV
                     FSYIPFASPLIMFERIVLSKVTFMEILITTIINVGFIILVGFFSSKLYKKGTLHYGKK
                     ASLIKIIRGKE"
     gene            complement(6425..7315)
                     /locus_tag="K8M77_002004"
     CDS             complement(6425..7315)
                     /locus_tag="K8M77_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI7086478.1"
                     /translation="MRVEAQNISKTFGEVQALKDLSFVIEKGKALGLLGRNGAGKTTA
                     IRILLGILPSDTGKVLVDNKKLSFDENAFGYLPEERGLYLKYTVKSQLMHFASLYGMK
                     KKEALNSIEYWLEKFEISEYLNKKVETLSKGNKQKIQLIVAVMHDPEVIILDEPFSGL
                     DPVNVELFKTVIRELLAKGKTLIFSSHRMADVEEFCDDIIMLKKGETILQGNLDKIKE
                     DYGIKGLVVEGEEKVQNFLKESGFEALEFKKGIYRVNLKDLEKGKELLRKITNTDLDI
                     RGFYFERPSLNDIFIERLGD"
BASE COUNT         2482 a         1313 c          728 g         2852 t
ORIGIN      
        1 ataatttcaa agttttaaaa cctaactcaa taaaaaaaga taaaacttat aaatatattc
       61 ttagagttaa tgaaatctac taagaaatta taagttttat ctttatattt tatattttat
      121 tttttatata ttctagtaat tttgatcgta ctgaagtcta tatctaaact tttctaattt
      181 atagtttcca ttcttatcta gttctttaag attatattta tgattaaatc ccattttctt
      241 ataaaaatca caggccatag ttgatgattt aacttcaact cttcttgctc ttatataata
      301 ttcatccctt tctagggtct ccataatttt tttgcctatt cccttacctt gaaattctgg
      361 taaaacaaat ataggtaata atatactttc atcttcttcc cctaaaaaac tagctatagc
      421 tccacaacca actattttat tttttaaaat agctacatac atatgtgagt ttttagccat
      481 attcaaaatt ttattttcag tatagttaga agctaaattt ctcatatctt cttttgaata
      541 accttttata tttgtatcta aaaaattttt ttcaataagt ttagaaactt ctcccccttc
      601 attttcttca aatcttcgta tttttaaatc ctccattaaa caattcacct gacttcctaa
      661 attaataatt accctaaaat atattaattg agttttttaa cttattttaa gcctatactt
      721 atcttagatt tatttttaat attaaactgt tactaaagtt aagaaaactc tctattaata
      781 cccaatttaa tgtaaattta ttacttttag ataaatttac gaacttctct cttatgataa
      841 aatttattat acaccttttc tctttattgt aaacattata aaactaatag tttcatgcta
      901 caaattgagt attcatgcca aattcattaa cacatcttat caaagataat aatttattta
      961 tttaaagcta taaccataaa aatctttata atatttaagc tttaaattta actgaattgc
     1021 tttattatga ataagtcact tccaatagta atgatcaatc ctattccagg agttgaaagc
     1081 gcaaattgta acttttttat gaagcataac ttaggtgtta agtctaactc acttcatgaa
     1141 actttaaaaa tttgcgaaaa acttatttct gataaaaatt tctatgagaa aatcgtatca
     1201 tctcaaaaac ttaattctaa tataaatgcc gcagaggata tatgtaaatt tttaataaca
     1261 aaataccatg aaattcaata caacagtgac aataataatc tttgactcat tgaaaaaatt
     1321 attgtaaact tataaataaa atctaagaat acaggtgatt ctattggcat ttattatttt
     1381 aggaatatat attttaataa atatagtttt acttcatgga acaaaaaaag ctagaaaagg
     1441 caaaaagacc gtaactataa aaataatgag ccttatttct ataattatgt ttatattgtc
     1501 attgctaata cttttatttg gttttatgtc ttatttacat cataaaaaca tgctaaattt
     1561 aataatatca attatttatt tatttgtttt aatttttata tcctataaat tatcatgttt
     1621 ttatataact gaagatacta taatctatgg atataactgc tacaacttta aagatataca
     1681 aaaaatagaa attgaaaagg ttaaaaataa gtttaatata acaatataca taaagggatc
     1741 aaatacatta tattttaagc tagattatct aaagaagaag aagcttttaa atgctcttca
     1801 cggtaaggtt ctgtgtataa atttataaac aaaaggctat ctaaacacac tacaaatatt
     1861 aatgtgttta gatagtcttt cttattaact tactcctaaa cttaaattat ataattttaa
     1921 tttaggctca acaaattttt cttccttaag attatagtta cagaaataat ataaaaatct
     1981 agctaactga atctctgcca tattaagtgc cttaatagct gctaaagctt gttctctagt
     2041 atcatcctta aaggcataat gaattaattc ttttgcatat tctgaacttt cattacctaa
     2101 atatctacaa taatcatcaa aatcaagatt actattttta taatatatat ctaaaacctt
     2161 tttcttatca tcttcattaa acaccttaat gccttcatcc tttagaatat aagaaaagat
     2221 ttcgccttta gtaggtactc cttttaaaag aatattttca tctttatcct tatttttatc
     2281 taataaaaga gttatatctt catctccctt tatccaagct tgtgctaagt tttcaaacct
     2341 acgatgatta gaaaaatttc cttttcctat tttatttata tcaaattttt ttaatacatt
     2401 cattattctt tcatttgggc tagcaatatg gtgagtaaca tggtgtggtt cactcatatc
     2461 agctaaatag tgacaagaat aacttaaata cttcatgcat tttcttctct tacctttatc
     2521 tctatacttt aaggcttttc gtccaaaaaa aataaattta cacattgctg ttttaccttt
     2581 tccaattctt ccaaagtaat tatccttatc ttcaccatga taaaaatgaa aagaaaataa
     2641 ggttaagact gacctaagca ttatgtctgg ctttctagaa tatgagggcc taatccatcc
     2701 taagctctta ctaacattat aattaagttc aagtatatcc atacaccatt taagagattt
     2761 agaaaaccac ttattattta aatacaaatt attatcatta atcaccattc tatttgcttg
     2821 ttctaataat acatcatggg tattagattt tttaaaagat tttttcataa cctttggcat
     2881 tttctttaat atctttttca tgttatccat gactgtcgcc tcccacctaa atacaattat
     2941 agcaccagac atagaaaata tttaacgtta aaaaaggcag taaaagctaa atttaagcct
     3001 ctactaccta ttaaaaagat ttatctattt cccttgcata ttatttcttt caacaaatct
     3061 tcttattctc tttatagcct ccattatgtc ttccattgaa gctgcgtaac aagctcttat
     3121 aaaaccttct ccacactctc caaaagcatt tcctggaaca gctagtacct tctcttcaag
     3181 aagaagtttt tcacaaaatt catctgaact cataccagtt gactttatac atgggaatac
     3241 atataaagct cctaatggtt caaagcaatc taatcccata cttctaaatc catcaactaa
     3301 tactcttctt cttctattat attcccttgc catttcaaaa acgctctcat caccattttt
     3361 aagagcctca atagcagcat actgagccgt tgttggtgaa cacattattg catactgatg
     3421 tatctttttc atagcatcta ttaaaacttt atgagcacat acatatccta atctccatcc
     3481 agtcatagca taagctttag agaatccatt tattactata gttttttcct ttacctcggg
     3541 gaaactagct attgaaacat ggtcctcatc ataggataat tctgcatata tttcatcaga
     3601 aataattatt acatccttat cctttaaaac atccacaatc ttttgaagct cttctttatt
     3661 cattattgct ccagtaggat tattagggaa tggaattatt accacctttg ttttctcagt
     3721 tatggcctct tctaaaagtt ctggagttaa tttaaaatca tcacaagctc ttaaatctat
     3781 tgtcttggca gttgctcctg taaatgctgt acatccctta taagcaacaa agcttggctc
     3841 aggtattata acctcatctc caggacctac taaagctcta agggctaaat ctatcccttc
     3901 acttccccca acagtaacaa gtatttcatc ctttggatta tatctaagag aaaatcttct
     3961 gtttaaatac tttgatatct cttctcttaa ttctataaat cctgcatttg atgaatagtg
     4021 agtatgtcct tgctctagag aataaattcc tgcctctcta acattccatg gagttacaaa
     4081 atcaggctcc ccaaccccta aagaaataac atcttccatt tcatttatta aatcaaaata
     4141 ttttctaata cctgaaggtg gcatattttt tacattatct aatatcatat tctctagtat
     4201 catataaata acatctccct atcatctgtt ttcttttctc taaatattgt tccatgatct
     4261 ttgtattttt taagtacaaa gtgagttgct gttcctgtta catattcttg aattgaaagt
     4321 ttatctgaaa caaacattgc cacttctttc attgtttttc cttctacaat aacagttaaa
     4381 tcaaatccac ctgaagacat taaataacaa gctttaacct gtggaaactt atatattctc
     4441 tctgcaacct tatcaaaacc aacacctctt tgaggagtta tttgtacttc tatcaaggct
     4501 gtaactgtct ctcttcctgt attctcccaa tttataagag tagtgtaacc agcgattatg
     4561 cttctttctt cataatctct tatggcttct ctaacctctt ccactgtttt ccctgtcata
     4621 actgcaattt cttgatctgt atacctactg tttttttcta aaacttctaa aatttcttcc
     4681 atgacacatt ggcccccttt gttattgctt attttctatt taaattgttt gattgtgttt
     4741 agcataatta ataaaaaaag ttcctccatc cctaaaaagg gacgaaggaa ctacttcgcg
     4801 gtaccaccct aataagtaaa attactttta ctcacctcag ttagaaatct ttaatagtat
     4861 ggctgaaatt taataaattt ataccttatt tattactcaa tttctatcct ctttaacggg
     4921 aggaaatcgt cttaatctaa ttaaaagtta tgtacatctt aattttaaaa catacttact
     4981 aaaatactct atttaacctt tagttctcaa caagaaactc cgagactgct tcaattaagc
     5041 tttcttacta aatctcacca aactttagct ctctgaaaag tccacctaat ttactctttc
     5101 tcttcactgt ttttcatgat tttttcgttt ttataattat atacacaaaa tttaataaat
     5161 tcaatatttt tttatattta tttataataa attattatat ttacaaaaat tttattcttt
     5221 acctcttata atttttatta atgaagcttt tttaccataa tgtaaagttc cttttttata
     5281 tagtttacta ctaaagaatc caactaaaat tatgaatcca acattgataa tagtagttat
     5341 taaaatttcc ataaaagtaa cttttgataa aactattctc tcaaacatta ttaaaggaga
     5401 agcaaatggt atataactaa atactttagt taaggttgat tcaatattaa acatacagtt
     5461 cattgcaagt aagaaaactc caacaaagac taaggtaatt gctcccattg atgaattgac
     5521 atcttgagaa cttgtagcta aggaagccac tgctgcataa agcattgaga atgttaaata
     5581 tcctaaaagg aaataggcaa caaaagcaaa tattatacta gcatttaaat ttaatgaact
     5641 taaaacttct aaacttgcac ctgaagtttt aagcattatt aagccagagc ctaggattac
     5701 tgctatctgt gtaagcccta cagcacaaat tcctaatacc ttaccaaaga aaagttccat
     5761 tggtttagcc atagttatta aggtttccat tattctatta ctcttttctt ctataactga
     5821 attagcaacc atagaaccaa aagtaagagt tattatataa attccaaaaa caagagcata
     5881 aactaaagcc attctctcat gtgaagcatt tcctgtttga accacatcaa gcttggctgg
     5941 agtcattata ttagcatatt cttctggtgt tatattaaac ttctccattg aaaatacaaa
     6001 tttagtgtca tttaaaattg tatttacttt cttcattaaa gaatctccag gtagttcatt
     6061 aacatataat tttcctgaca cctctttacc tgttgaaact tccagtaaag ccttataatc
     6121 tgactttcca ttttctattt cctttttaac cttattagtt tcttctgaat ctttgcttaa
     6181 aacaacttta aaatcatcat catttaaact ttctaaagta ttagagtcaa aaaatttatt
     6241 attagcttct gtgcttatca ttattaaatc tttagagcct tccctattaa atagccctac
     6301 tattttatcc atattaaaga atcctagcat tacaattaaa gtaattattg ttgatattat
     6361 aagagcttta tttctaatag tctctttata ggtaaaagta aaaatactcc aaaacttttt
     6421 catactaatc acctaacctt tctatgaaaa tatcatttaa ggatggtctt tcaaaataaa
     6481 atcctcttat atctaaatct gtgttagtga tttttcttaa aagttctttt cctttttcta
     6541 aatccttaag atttactcta taaattccct ttttaaattc taaagcttca aaacctgatt
     6601 cttttaagaa attttgtact ttttcttctc cttcaactac aagaccttta attccataat
     6661 cttctttaat cttatcaaga tttccttgaa gtatggtttc ccctttttta agcattatta
     6721 tatcatcaca aaactcttcc acatcagcca ttctgtgact actgaaaatt aatgttttgc
     6781 ctttagctaa tagttctctt attacagttt taaataattc cacatttaca gggtctagtc
     6841 cactaaatgg ctcatctaaa attataactt ctggatcatg cattactgca actattaact
     6901 gtattttttg tttattacct tttgatagag tttcaacctt tttatttaga tattcactta
     6961 tttcaaattt ttcaagccaa tactctatgc tatttaatgc ctcttttttc ttcattccat
     7021 aaagagaagc aaaatgcata agttgactct taactgtgta tttaagatat aatcctctct
     7081 cctctggtaa atatccaaaa gcattttcat caaagcttag ttttttatta tccactaaaa
     7141 cctttccagt atcacttggt aatattccaa gtaatattct tattgcagtg gttttacctg
     7201 ctccatttct tcctaataac cctaaagcct ttcctttttc aatgacaaag cttaaatctt
     7261 ttaaagcttg tacctctcca aaggttttgc ttatattttg agcctcaact ctcactaaaa
     7321 atcccccttt tcattttgat aaacatgtta aaatttactg ttacataaaa tatta
//
LOCUS       DAEVGI010000123         7270 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEVGI010000123 DAEVGI010000000
VERSION     DAEVGI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7270
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.123"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(314..973)
                     /locus_tag="K8M77_002005"
     CDS             complement(314..973)
                     /locus_tag="K8M77_002005"
                     /inference="COORDINATES: protein motif:HMM:NF037930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086479.1"
                     /translation="MARSKLIKDFINSQMDINTALQNLMAILYCLEDETLITWTKKEL
                     SGYNFDDSLPQYRLLSGIVMANFIVGNMKYTKYQFPINHLDKNFQRSLLEVPILSSIS
                     TLNETIKKDFNLVKPISPELYPMLQSNSNANIIKAYVDINIVNLTDIISKVKTKILET
                     LLFLEKEFGNLDNLDIDISSKTSENIKNIVHHIQINLYDNSISIGDNNKIKNSDILTN
                     K"
     gene            complement(986..1192)
                     /locus_tag="K8M77_002006"
     CDS             complement(986..1192)
                     /locus_tag="K8M77_002006"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086480.1"
                     /translation="MGIKIGDNNRIKKSNIIDSSNSNVENTNKDSFPNRHPILIGILC
                     SIIASIIMMFSFWDKISYFIKNLL"
     gene            2504..2764
                     /locus_tag="K8M77_002007"
     CDS             2504..2764
                     /locus_tag="K8M77_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005215132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086481.1"
                     /translation="MAVDKKKRNRIIINFKDTKEEMELYEKLLKHSNPASYIKDIFMG
                     LIPLTLEDNIQVVQKSTECNKEENENSEEILDIDNILGIIDN"
     gene            3261..3683
                     /locus_tag="K8M77_002008"
     CDS             3261..3683
                     /locus_tag="K8M77_002008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086482.1"
                     /translation="MKQENRVLLIFEDKFIIGDNISLIKDYMKDSFNVKITKWTIQGI
                     PKVHRNKVKYLGTLLDYTNPITKNDNLIRLVKNQNNSQKIYRDSFVEIEKEKLRYKKK
                     LYNESYKELVDGGIIKFVNKEIIDDMIYQSKLRGCLIG"
     gene            3972..4388
                     /locus_tag="K8M77_002009"
     CDS             3972..4388
                     /locus_tag="K8M77_002009"
                     /inference="COORDINATES: protein motif:HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086483.1"
                     /translation="MLQDFFTDDNEYVRLPKEELFIQTKLTFIRNPNYNIYQKMLYQL
                     LHTYGIDKRGVYPSQGKLAKELGISRRKVISTLQELEDLGGIYIINQVFENTKHKTSN
                     LYYISEIDKEGNFNKDSLKIVKKMYSNKTRVLDKPY"
     gene            4538..5221
                     /locus_tag="K8M77_002010"
     CDS             4538..5221
                     /locus_tag="K8M77_002010"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013691.1,HMM:NF025107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086484.1"
                     /translation="MNKNHYVYLITNLINGKKYIGKISCNCPIEEDQYMGSGKILRNA
                     KLKYGNENFIKSIIEVCNTENDTYEREKYWIEEYNAFDSNEFYNLTPGGEGNSTYNRF
                     EVIDNNIAKFKDDNMYINNAFMRNKELNMQEKSFYIYVRGYGDKCFANQSTICDDLAI
                     SRPTLRKLMKSLEDKSYIYVQRKYSAGTKEKATPVIFPIPLDENTGLLTEYHKEIIEY
                     TKYKYPSDY"
     gene            complement(5266..6048)
                     /locus_tag="K8M77_002011"
     CDS             complement(5266..6048)
                     /locus_tag="K8M77_002011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086485.1"
                     /translation="MKKIYFAKLFIVSILIMIINNIVNLFDNAPDFFKYGDSLFHFLQ
                     NILSIINQLASSTAAAVIFYYCVEFLNKNKELDKYRDFRSDLYHVLYSYLESTKNIKG
                     FEVFKGRQVKPGDMFTYKDVPIFINLVKSFDTNTIINNISSYLCTSESVKVVEFIRSI
                     NFGLGKLINHKNYRYLKNSTEKLQNISDLYEFDLYGSDEFFNNIVKKSINKEEDTRNY
                     ILELIDYYIYVLDDIIFLYENTDKFIESIEKRHIWTFITLLD"
     gene            6334..>7270
                     /locus_tag="K8M77_002012"
     CDS             6334..>7270
                     /locus_tag="K8M77_002012"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012464.1,HMM:NF019149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7086486.1"
                     /translation="MVAIYLRKSRGDIEDLNKHKETLIELCNNNSWKYDLYEEIGSSA
                     TLERPELSKLLSNINNYTKVVVMALDRLSREELGQATITNLFRENNIEVVTPTKIYNF
                     NKEQDTLMSDFEKLLARQEFRLIKKRLVTGKIQSFKQGNWVQGFAPMPYVYNRDTKSL
                     DIDKEKFEVFERIKELALKGYSAQIIACDVNMKVITIRRLLKNKTLLGYVKYKGEYVK
                     GNHEPVLTLEEWNIIQNYIKGRTNGITRTRHHYPLTGLVKCNCGHSRSSVRRKDRNNK
                     EIIAKCRYCNDSSMLSDNIHQKIKEELNVEIDRITF"
BASE COUNT         3057 a          683 c          981 g         2549 t
ORIGIN      
        1 aaagacattt ctaataattt attaaagacc atattaaatt ataaattact atagtaattt
       61 atatgcgtta tcaaagtatc actaatatta aaagaattat attgtcaata acaaataaat
      121 ttttaggaat aaataaaaag taatatagaa tacttcaaat agtctaactt tttaataact
      181 ttaagttata tgataataaa tttattttga ttttagagag tattataatt aaactaactt
      241 acgtatttat ttaaaataaa aagagagtaa aactctcttt atttaaatag cttttaattc
      301 aatatactaa attttatttg ttagttaata tgtcagagtt cttaatctta ttattatcac
      361 caatacttat gctattatca tatagattaa tttgtatatg gtgaactata tttttaatat
      421 tttcagaagt tttactagat atatcaatat caagattatc gagatttccg aattcttttt
      481 ctaagaataa gagagtctct aaaattttag tttttacttt agatataata tctgttaaat
      541 taactatatt aatatctaca taagctttta taatatttgc gttagaattt gattgtaaca
      601 taggatacaa ctcaggagat ataggtttta ctaaattaaa atctttcttt attgtttcat
      661 ttaaagttga tatgcttgaa agtatgggaa cttccaataa agatctttga aaatttttat
      721 ctaaatgatt aataggaaat tgatatttag tatatttcat atttcctaca ataaagttag
      781 ccataactat acctgataat aatctatatt gtggaagact atcatcaaaa ttatatccag
      841 atagttcttt tttagtccaa gttattagtg tttcatcttc taaacaatat aaaatagcca
      901 ttaaattttg aagtgcagta ttaatatcca tttgtgaatt tataaagtct tttattaatt
      961 tgcttcttgc caatctaact accccctata ataaattttt aataaaataa gaaattttat
     1021 cccaaaatga gaacatcatt attatacttg ctattataga acataaaatt cctattaata
     1081 taggatgtct gttaggaaac gaatctttat ttgtattttc aacgttacta ttactactgt
     1141 ctattatatt agatttttta attctattat tatcaccaat ttttataccc ataaattaaa
     1201 actcctttaa taagttatga atattttacc atataaaaat atgtttaaaa gagaataaga
     1261 aatataacag tgagcattaa aataaggtaa gataggagac ttcaaaattt tatttttata
     1321 taatatttaa gtcattaaat aagtgcttag tataaaaaat agatatggta gttactttaa
     1381 taaatgctat ggtattttga gaaaaagact ttgcaggtgg aataaatgtt tatgaagatg
     1441 aaaatgaaag aattggattt attatattat aaaatgaact ttgttaactt tatttatata
     1501 atataaatat aaaattaaat aattggagtt tcagagctga cattatctcc attatcaaaa
     1561 aatgtaaagg tgaactagag ccgacgcaat aagtctagtc ctgtattatt cagtagcgta
     1621 ataagacata aagaactact cggtaatacc agatatagta ggaaagctat atcaatttta
     1681 aaaaatggtt aatagtttta atattctata taaaatattg gaggaaaaat gtgaaattag
     1741 ccttaaaaac tttagaaagg cttaggtgat ttaaagaatt tggttaattt cactcctaag
     1801 attactgaat taatggtttt atttacataa aaccattaaa acattataaa aaataaagag
     1861 aaatttcata aaaagtattc attattatat tctctcttta tattagaaat atagtatttt
     1921 acacattata taatattata caaggtgtat tagattgtat agtattagta catattttac
     1981 aaaatataaa tatatgtata atataaacaa atacaaagtg tataaaagta tactgatgat
     2041 acaatattaa ttaaaatgta atttggaaat tactgggttg gatattaagg aaataaatac
     2101 ttcattggta caatagaata caaaattaat ttagacaagt ttaatactta aatagtaatg
     2161 ttatcagcat tagaatctta tagggataac ttctttaagt tatttattaa tttaacaatt
     2221 agatattata agataggtaa agatgaatca agaaagaggt taatttataa aaaaatctat
     2281 gactttagag ctgataaaag ttataagaag aaaataattt atctattgaa taaatagaat
     2341 atattctaaa acataggata gaagcagata gagaaaagga aaatatataa tttgttaatc
     2401 atattattag agaatataca caaagatttt aaatgaagtt acacttaata aacttcatgc
     2461 taaatgctaa tgcagaagga tattataaga taggggtgag tacatggcag tagataagaa
     2521 gaaaaggaat aggataataa ttaattttaa agatacgaaa gaagaaatgg agttatatga
     2581 aaagctttta aaacattcaa atcctgcatc atatataaaa gatatattta tggggttaat
     2641 tccattaaca ttagaagata atattcaagt tgtacaaaag agtacagaat gtaacaaaga
     2701 agaaaatgaa aatagtgaag aaatcttaga tatagataat attttaggaa ttattgataa
     2761 ttaaagtaaa aaaatctacc atcaaatgaa ggtagattag tggtaaagtt atttatttat
     2821 tacacttcgt tatataaata aatatattct gcaaacttca aaaatataac tatttttagt
     2881 taaaaatatg tataattaac agaatattaa tttatctgat tagtgtttca gctattcttg
     2941 ctcctgcact taaagctaaa agagctagtt ctattgcaaa catagttgtc attttaatca
     3001 cctcacaaat tattgggtta tttatatttt aattatattt taaaggttta tacacttaag
     3061 agagattata gtgttattaa atttgagtag aggatatttt aaggttaatt tataatagag
     3121 atagaattag aagaaaaaaa taatacatca actactacag atattagctt tcttttaaat
     3181 gaactttata acaacaattt atataatata aatatatcaa taaatactat gtaatgaatt
     3241 gttaaaggag aatgataaag atgaaacagg agaatagagt actgttaata tttgaagata
     3301 aatttattat aggggataat atttctctta ttaaagatta tatgaaagat agttttaatg
     3361 tgaagattac taaatggaca atacaaggaa tacctaaggt acaccgaaat aaagttaagt
     3421 atttgggaac tttacttgat tatacaaatc caataactaa aaatgataat ctaataagat
     3481 tagtaaaaaa tcaaaataat agtcaaaaaa tatatagaga tagttttgta gaaatagaaa
     3541 aagaaaaatt aagatataag aaaaaattat acaatgaatc gtataaggaa ttagtagatg
     3601 gaggtattat taaattcgta aataaagaaa taattgatga tatgatttat caatcaaaac
     3661 ttagaggatg tttgatagga tagctattaa tagctatctt tttctttttt atatagttat
     3721 attgtgcata atattcacta tgtatatgaa taatgttcac taatattgta actaatatgc
     3781 ataatatatt gtgaataatg ttcacaagga ttgtgaataa aagttacata aaatatataa
     3841 atataaaaat aaataaaaat ataaatataa aaaataaata tgtacgaaac tcctttttct
     3901 atttataatt atactatatg gttattaaaa ctatattaaa ggattataaa aactgtaaag
     3961 gagttatttt tatgttacaa gattttttca cagatgataa tgaatatgtg aggttaccca
     4021 aagaagaatt atttatccaa actaaactca cttttattag gaacccaaat tacaatattt
     4081 atcagaaaat gttataccaa ctacttcata cttatggaat cgataaaaga ggtgtttatc
     4141 caagtcaagg aaagttagct aaagagcttg gtatcagcag aagaaaagtt atatcaactc
     4201 tacaagaatt agaagattta ggaggtattt atattatcaa tcaagtattt gagaatacta
     4261 aacataagac aagcaacctt tactacatat cagagattga taaagaaggg aattttaata
     4321 aagattcatt aaagatagta aaaaagatgt attctaataa aacacgagtt ctggacaaac
     4381 cgtattaaac ttattaaaat acaccaatca ctttaaacta attataacag ataaaatgta
     4441 tttaataaat attttttatc tgtttaattt taactagata tgaactaaga taagtcgatt
     4501 tatataataa taaatgattg ataggagaaa ttaaattatg aataagaatc attatgttta
     4561 cttaataact aatctaataa acggaaagaa atacattggt aaaataagtt gtaattgtcc
     4621 aatagaagaa gatcaatata tgggaagtgg aaaaatatta agaaatgcca aattaaaata
     4681 tggtaatgag aattttatca aatctattat agaagtgtgt aatacagaaa atgatactta
     4741 tgaaagagag aaatattgga tagaagaata taatgctttt gatagcaatg aattttataa
     4801 tttgactcca ggtggagaag ggaattcaac ttataacaga tttgaagtaa tagataataa
     4861 tatagctaaa tttaaagatg ataatatgta tattaataac gcttttatga gaaacaaaga
     4921 attgaatatg caagaaaaat ctttttatat ttatgtaaga ggttacggag ataagtgttt
     4981 tgctaatcaa tctactattt gtgatgatct agctatatct agacctacat tgagaaaatt
     5041 aatgaaaagt ttagaagata aaagttatat ttatgttcaa agaaaatatt cagctggaac
     5101 aaaagagaaa gcaaccccag ttatttttcc aataccactc gatgagaata caggattgct
     5161 tacagaatac cacaaagaaa taatagaata cactaaatat aaatatccaa gtgattatta
     5221 aatactatat cattccacta gagaattatc tagtgggatt tttatttaat caagtaaagt
     5281 tataaaggtc caaatatgtc ttttttcaat ggattcaata aacttatctg tattttcata
     5341 taagaagatt atatcatcta aaacataaat ataataatct attaattcaa gaatataatt
     5401 tctagtatct tcttctttat taattgactt tttaacaata ttattaaaaa attcatcact
     5461 accatacaaa tcaaattcat ataaatctga gatgttttga agtttttcag tagaattttt
     5521 taaatatcta taatttttat gatttattaa tttacctaat ccaaaattta ttgatctaat
     5581 gaattcaact acttttacag attctgaagt acataaataa gaagatatat tattaattat
     5641 agtatttgta tcaaaggatt tcactaaatt tataaatata ggaacatctt tataggtgaa
     5701 catatctcct ggcttaactt gtctaccttt gaatacttca aaacctttga tattttttgt
     5761 tgattcaaga taggaatata atacatgata taagtctgat ctaaaatctc tatatttatc
     5821 taattcttta tttttattta agaattctac acaataataa aatataactg cggcagctgt
     5881 cgatgaagct agctgattta ttatagataa gatgttttgt aagaagtgaa ataaagaatc
     5941 accatattta aaaaaatccg gagcattatc aaatagatta acaatattgt ttattatcat
     6001 aattaatata ctaactatga ataattttgc aaaataaatt tttttcataa ttcctcccaa
     6061 aataaattaa tattaatttc aaataaagta tatcaaaatt aattagcact taacatatat
     6121 ttcatatgaa ctcttatatt attttttata taaataatag taggaacgtt ttttagctag
     6181 aaaacttcaa attggatttt ggaggtatta tatagtagat gaataaaggt aataataaaa
     6241 ttagtgataa agcaatgaag gaatttacac aagaattaaa gagattatat tataagtata
     6301 agggtaataa tataaagaaa gtgagttgat attttggtag ctatatattt aaggaaatct
     6361 aggggagata ttgaagattt aaataaacac aaagaaacac tcatagagtt atgtaacaat
     6421 aatagttgga aatatgattt atatgaagag ataggtagtt cggcaacact agaaaggcca
     6481 gagttaagca aattactatc taatataaat aattatacta aagttgtagt aatggcgttg
     6541 gatagacttt caagagaaga acttgggcaa gctactatta caaatttatt tagggagaat
     6601 aacattgaag tagtaacacc aactaaaatt tataatttta ataaagaaca agatacatta
     6661 atgtctgatt ttgagaaact actagcaaga caagaattta gattaatcaa gaaaaggctt
     6721 gttactggga aaattcagag ttttaaacaa ggaaattggg tacaaggttt tgcacctatg
     6781 ccttatgtat ataatagaga cactaaaagt ttagatatag ataaagaaaa gtttgaggtt
     6841 tttgagagga ttaaagaatt agctttaaaa ggttattctg ctcaaattat tgcttgtgat
     6901 gtaaatatga aagtaatcac tattaggaga ctattaaaaa ataaaacttt attaggatat
     6961 gttaaatata aaggtgaata tgtaaaaggc aatcatgagc cagttttaac tttagaagaa
     7021 tggaatatca ttcaaaacta tataaaaggt agaactaatg ggattactag aactagacat
     7081 cattatccat taacagggtt agttaaatgc aattgtggac atagtaggtc ttccgtaaga
     7141 agaaaagata gaaataataa ggaaattatc gctaagtgta gatattgtaa tgatagttct
     7201 atgttatcag ataatataca tcaaaaaata aaagaagaat taaatgttga aattgataga
     7261 ataacgtttg
//
LOCUS       DAEVGI010000124         7220 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEVGI010000124 DAEVGI010000000
VERSION     DAEVGI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.124"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..268)
                     /locus_tag="K8M77_002013"
     CDS             complement(<1..268)
                     /locus_tag="K8M77_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086487.1"
                     /translation="MDREILKGSLEIILLSLLKNKDMYGYEMSKEIKNITENVLILGE
                     GTLYPALKRLKEKNLIQDYFIETNSSKKKRKYYKITEAGLIELNL"
     gene            493..963
                     /locus_tag="K8M77_002014"
     CDS             493..963
                     /locus_tag="K8M77_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086488.1"
                     /translation="MNLVKTRDDLEREAPRLKKEWIQKIDSIDNANRKYVLVFEDLVF
                     EADNEQDITSRLIRDYIETDDRNMQLLFRIDFARALSMYSIMNGINVEVYNNGKKVRN
                     NYAVSEDDPDYEKDYEIPDVILDVFDEFTLFKGLNELKYAKIYYKSDDGEYKLF"
     gene            1030..1560
                     /locus_tag="K8M77_002015"
     CDS             1030..1560
                     /locus_tag="K8M77_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI7086489.1"
                     /translation="MKKREQIYTFLIDFIKEKGYQPTVREIAHAVNLKSSSTVYKHLE
                     MLEKDGLIKLGKREQRGRKRSIHIIDLNKKIKKNVSEETIKNLIKFSKKQVNTNNDIF
                     SKKVTNDYIVAVDNIFPDDIIIVEKVNSLKKDSIGLLLFENRTFIKKIFSDYEKVYNT
                     DPIVLGKIIGVYRDFF"
     gene            1846..2073
                     /locus_tag="K8M77_002016"
     CDS             1846..2073
                     /locus_tag="K8M77_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086490.1"
                     /translation="MRLSEEDKKCIKILYRNGLNVKETEKLLNNDFSKDAIKKHIYRH
                     LNEFRNEHIINISLNKNKLKKEFKNIIKNNF"
     gene            2139..2401
                     /locus_tag="K8M77_002017"
                     /pseudo
     CDS             2139..2401
                     /locus_tag="K8M77_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463356.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(2654..2827)
                     /locus_tag="K8M77_002018"
     CDS             complement(2654..2827)
                     /locus_tag="K8M77_002018"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086491.1"
                     /translation="MKKNTKQILVSSILGASIALIVCLLFDYFGIPKNIFSYIFILII
                     FLVISRSINNKYI"
     gene            complement(2827..3198)
                     /locus_tag="K8M77_002019"
     CDS             complement(2827..3198)
                     /locus_tag="K8M77_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086492.1"
                     /translation="MSSIALQLPNSYVELEREEIEYVDGGFAVGVNFAGHAFNLIIAA
                     ICGGATASVSTFISSVGKKEAKKIFTRNIKSKLLEWGCERLAGKVAFAVDVALEFSDV
                     GGAIARFLDAHDTYPNNGWIG"
     gene            complement(3265..4377)
                     /locus_tag="K8M77_002020"
     CDS             complement(3265..4377)
                     /locus_tag="K8M77_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HBI7086493.1"
                     /translation="MKIYSINELTHSKILYDKNPPKFMMYIIIIVLALLVAFIVWSTK
                     SVKTYIIKGQGIVTTDTKSNVMTKMSGEITDVFIEEGKDVNEGDTLISLNPLESNLQL
                     GQIQAQIQVINNRITLLDRAEKDATNGRNTFNKNNSDEVEFYNKLANLNTKRKEFIVD
                     KEEAKKQGATDEQIEKYEKSQKNKDDVTRYEGILQFTNEKKQLEFEKSKLEAQKKSLE
                     EAAEEFKIVAPKSGKLHLNVPLNKGMVLQAGSLIGTLTNKEEKIIIESLIPSNERPRI
                     HIGDEVSLAVLGLSQSEYGTIEGQVSSIDEDATIDNEKGNVFFKIKIEPQKTYLTDTK
                     GEKVNLTLGMLTETRVKYEKITYMKYFLDQLGIKIN"
     gene            complement(4364..4867)
                     /locus_tag="K8M77_002021"
     CDS             complement(4364..4867)
                     /locus_tag="K8M77_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086494.1"
                     /translation="MNTNMYKKITLKENKTLKLKNVLSRELTQSEFMNENKIMYMMES
                     YIKSKGNSIVGPMINYSSIISGENGQPKLVAKLMVQLKETIENLEEPYEFSSQIRVPN
                     CLFARFIEKEQNLQFAYAKLNLHAFENNIKLLGDNYTIFVERNKNNIVADIFMQMQNE
                     VGPIENI"
     gene            complement(4848..7130)
                     /locus_tag="K8M77_002022"
     CDS             complement(4848..7130)
                     /locus_tag="K8M77_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HBI7086495.1"
                     /translation="MFYNNKYLIKQHDSTDCAAACLASVCMYYKKEITITKLRDILGT
                     DIKGTTLKGLENGANRLGFDTKAIRVDKSGFMSKFTLPAIAHIVTDEGLSHFVIIHKI
                     KNNKVIILDPAKGKEKENINEFFKRFDGILLLLAPNNDFSLDRSKEEGTLSKFIKILL
                     PQKKLFICSIISSVILTVLGISTSFFNKILIDEILPYGLKNQLIIFCICFITLGVTQV
                     LISAIRQHMLLYLSQKIDIPLLLGYFKHVYKLPTKFFETRKVGDILTRFSDAFVIKNI
                     LTSVTLSLIIDIVLTVVSAIILCLMNKTLFLVVIILTLISASLIFIFKIPYKKINLKQ
                     MEASSSLNSTIIESLRGIDTIKYHAAEEKTIEKLEKEYIKNLKITFQEGVLSNIQGSI
                     SSFISYTGNLALMSIGATMVMSGKTTLGSLMAFTTLSGYFMGPINRLIGLQLSIQEAS
                     ISLKRISEIYEVEEEQEENDNKFKIKKIDGDIVLKNITFRYGSRSPVLKNLNLTIPKG
                     KKVALVGESGSGKTTISKLLLKSFDVEEGSIKISGKDINDIDLYSLRKAISYVPQNIE
                     LFSGTIKDNVILGKSNVTNEQISIACENAGCSSFIEKLPGKYDTFLEEAGRDLSGGEK
                     QRIAMARALIKDTDLLILDEATSNLDFISEARIYNTLFVKCKSKTMLIIAHRLSTIRN
                     CDIIYVLHKGEVVEQGNHQSLLDKKGYYYKLYVSQVGEISIEKKDRDKTIIKEESNIK
                     IVDYNIENLEDMENDEYEYV"
BASE COUNT         2592 a         1013 c          774 g         2841 t
ORIGIN      
        1 ttaaatttaa ttcaattaaa ccagcttcag taattttata atattttctt tttttctttg
       61 aactattagt ttcaatgaaa taatcttgta ttaaattttt ttcttttaat cttttaagtg
      121 ctggatataa agttccttcc cctaatatta aaacattctc agttatgttt ttaatttctt
      181 tagacatctc atatccatac atatctttat tctttaataa agatagtaaa attatttcta
      241 atgaaccttt taatatctct ctatccaaaa aatcaactcc ctttaagtta atttagtgtt
      301 ctataattta attttacgaa aacatacatt gctttacaag gtattaatta aaaaatatat
      361 tatatttttt aattaatttc agaaaatact taaagtatta taactcagaa ataaaaaagt
      421 taaagcaaaa agctttaact tttttatttc tgagttataa tgtgagtaaa aatattgaga
      481 ggtgtattaa gtatgaattt agttaaaaca agagatgatt tagaaagaga agctcctaga
      541 ctaaaaaaag agtggataca aaaaattgat agtatagata atgcaaatag aaaatatgta
      601 ttagtttttg aggatctagt attcgaggca gacaatgaac aggatattac ttcaagacta
      661 ataagagatt atatagaaac tgatgataga aatatgcagc ttctatttag aattgacttt
      721 gcaagagctt taagtatgta tagcataatg aatggaatta atgttgaagt ttataataac
      781 ggtaagaaag taagaaataa ctatgctgta tctgaagatg atccagatta tgaaaaggac
      841 tatgaaattc ctgatgtaat cctggatgta tttgatgaat ttactctttt taagggcctt
      901 aatgaactta agtatgctaa aatatactat aaatctgatg atggagagta caagctattt
      961 taatataaat taaaataaag aagtagcttt aagtaataaa aagctgcttc ttttttataa
     1021 ggtgaacata tgaaaaaaag agaacaaata tatacatttt taatagattt tataaaagaa
     1081 aaaggatatc aacctacggt tagagaaatt gctcatgcag taaatttaaa gtcatcatct
     1141 actgtttata aacatttaga aatgttagaa aaagatggat taataaaact tgggaaaaga
     1201 gaacagagag gtagaaaaag aagtattcat attattgatt taaacaaaaa gattaaaaaa
     1261 aatgtatcag aagaaactat taaaaattta attaaatttt caaaaaaaca agttaatact
     1321 aataatgata tattttccaa gaaagttact aatgattata tagtagctgt agataatata
     1381 tttcctgatg acattattat cgttgagaaa gtaaattctc taaaaaagga tagtatagga
     1441 ttattattat ttgaaaatcg tacctttata aaaaaaatat tttcagatta tgaaaaggta
     1501 tataatactg atcctatagt tttagggaag attattggtg tttatagaga ttttttctga
     1561 tattacttga aaaaacataa aagaaacgtt aaaataggtt aacaatatta aaatttactg
     1621 taataatgta ttagaaactt tcaagtttat cagttaatag ttaaaccagt attaactatt
     1681 aaattaatta caaacttaat ttaatctgta tatctattaa cacaaaaaag attatctctt
     1741 taattttaga gataatcttt tttgtgttta gaattaacca agaatattat acaatttaat
     1801 tatatcctat actcatagta attataaatt ggggtaagaa aatttatgag attatcagaa
     1861 gaagataaga aatgtataaa aattctttat agaaatggct taaatgtaaa agaaactgaa
     1921 aaattattaa ataatgactt ttctaaagat gctattaaaa aacatatata tagacattta
     1981 aatgaattta ggaatgaaca tattataaat atatccttaa ataaaaataa attaaagaaa
     2041 gaatttaaaa atattattaa gaataatttt taataaaggt ttttccataa ttttcaagaa
     2101 tattatttat atattcttaa tttagggaaa attaagatat gagaacagct caagaagtag
     2161 ccaatgaatt agatttaacc aggactaaag tttataattt gctttattct ccattagtta
     2221 aaaagaatgg tggtcaagta gcaataagca atgaactttc aaattattag aagaaaaaaa
     2281 tactatagct gatgtaaaag ctgaagaaag tattgatttt acatcaacta tagaaattct
     2341 acaaaatcaa ataactattt taaataatat tataactaat aatttaaata gaatactaga
     2401 ccactagcaa ttaaatattt atctcctcat tttttctcaa gagaaattaa agcttgaagt
     2461 gtaacatctt aagcagtctt tatattagta tttttagtaa ttttttatca attctttttc
     2521 ggcataatac atagtctatg caccctcctt ttaaatgata attaattata tcattttagc
     2581 agtttacaca gactatttta caatcttgct tagttattat aactttatgt atctctactt
     2641 ataagctttt cacttatata tatttattat ttatactacg tgaaattact aaaaatatta
     2701 ttaaaataaa aatataagaa aatatatttt taggtattcc aaaatagtca aataataaac
     2761 atactattaa tgctatacta gctccaagta ttgatgaaac taatatttgt ttagtatttt
     2821 tcttcattaa cctatccacc cattattagg atatgtgtca tgagcatcta gaaatctagc
     2881 tatagcacct cctacatcac taaattctaa tgctacatct acagcgaaag caactttacc
     2941 agctaatctt tcacaacccc attctaacaa tttacttttt atatttctag taaatatttt
     3001 tttggcttcc ttttttccta cactagatat gaatgtactt actgatgctg tagcaccacc
     3061 acatattgct gctattataa gattaaaagc atgtccagca aaatttacac ctactgcaaa
     3121 tcccccatca acatattcta tttcttctct ttcaagctcc acataactat taggcaattg
     3181 taatgctatt gaactcatct taagctcctc ctttttttct ttaaatttta tatattttaa
     3241 aggtatttta ctacctaata aaagctagtt aatctttatt cctagttggt ctaagaagta
     3301 tttcatatat gttatttttt cgtatttaac ccttgtttct gtaagcatac ccaaagttaa
     3361 atttactttc tcaccttttg tgtctgtaag gtatgttttt tgaggttcta tctttatttt
     3421 gaaaaataca tttccttttt cattatctat agtagcatct tcatcaatac ttgaaacttg
     3481 tccttctata gttccatatt ctgattgact taaacctaat accgctaggg acacttcatc
     3541 acctatatgt attcttggtc tttcatttga tggaattaaa gattctataa taatcttttc
     3601 ttctttatta gttaaagttc ctattaaact acctgcttga agtaccattc ctttatttaa
     3661 tggtacattt aaatgtaact ttccactttt aggtgctaca attttaaact cttcagcagc
     3721 ttcttctaat gatttttttt gtgcttctaa tttacttttt tcaaactcta attgcttttt
     3781 ttcatttgta aattgaagaa tcccctcata tcttgtcaca tcatctttat ttttttgtga
     3841 tttctcatat ttttctatct gttcatccgt agctccttgt tttttagctt cttctttatc
     3901 tacaataaat tcttttcttt ttgtatttaa attagctaat ttattataaa actctacttc
     3961 atcagaatta tttttattga aagtatttct accattagta gcatctttct ctgccctatc
     4021 aagtaaagtt attctattat taataacttg aatctgagct tgaatttgac ctaattgtaa
     4081 gttggattct aaaggattta aacttattaa agtatctcct tcattaacat cttttccttc
     4141 ttcaataaaa acatctgtaa tttcacctga cattttagtc attacatttg attttgtatc
     4201 tgtagtaact ataccttgtc cttttattat ataagtcttt actgatttag ttgaccaaac
     4261 tataaacgct actaataaag ctaacactat aattattata tacatcataa atttaggtgg
     4321 atttttatca tataaaattt tactgtgagt tagttcattt atactatata ttttcaattg
     4381 gacctacctc attttgcatt tgcataaata tatctgctac aatattattt ttatttcttt
     4441 caacaaaaat agtatagtta tctcctaata atttaatgtt attttcaaat gcatgtaagt
     4501 ttaactttgc ataagcaaat tgaagatttt gttccttttc aataaatctt gcaaacaagc
     4561 aattggggac tcttatttgt gaactaaatt cataaggttc ttccaagttt tctatagttt
     4621 ctttaagttg taccataagc ttagcaacta acttaggttg tccattttct ccacttataa
     4681 ttgatgaata atttatcata ggtccaacta tagaattacc ctttgacttt atgtaacttt
     4741 ccatcatata cataatttta ttttcattca taaattcgct ttgagttaac tctctactta
     4801 atacattttt taatttaaga gttttatttt cttttaatgt tattttttta tacatattcg
     4861 tattcatcat tctccatgtc ctctaaattt tctatattat aatctacaat ttttatgtta
     4921 ctttcttctt ttatgatagt tttatctcta tctttctttt caatagatat ttcaccaact
     4981 tggctaacat ataacttata ataatatcct ttcttgtcaa gtaaactttg atgatttcct
     5041 tgttcaacaa cctcaccttt atgtaaaaca tatataatat cacaatttct tatagttgaa
     5101 agtctatgtg caattattaa catagtttta cttttacact ttacaaataa agtattataa
     5161 atcctagctt cacttataaa atctaaatta ctagtcgctt catctaaaat taataaatct
     5221 gtatccttta ttaaagctct agccatagca attctttgtt tttctccacc tgaaagatct
     5281 ctaccggctt cttctaaaaa agtatcatac tttccaggaa gtttttcaat aaaagagcta
     5341 catcctgcat tttcacaagc aatagaaatt tgttcatttg taacattact ttttcctaaa
     5401 ataacattat ccttaatagt tcctgaaaat aactctatat tttgaggaac ataagatata
     5461 gcttttctta aggaatataa atcaatatcg tttatatcct ttccagaaat ttttatactt
     5521 ccttcttcaa catcaaagga ttttagtaaa agttttgata tagtagtttt cccacttcct
     5581 gattcaccaa ctaaagcaac ctttttcccc tttggaatag taagatttaa attctttaaa
     5641 acaggtgatc tactcccata tctaaaagta atatttttaa gaactatatc tccatctatt
     5701 ttttttattt taaatttatt gtcattttct tcttgctctt cttcaacttc atatatttct
     5761 gaaattcttt ttaaagaaat actagcttct tgtatactta gctgtaaccc aataagtctg
     5821 tttattggac ccataaaata tccagaaagt gttgtaaagg ccattaaact tcctaaagtt
     5881 gttttcccac tcataaccat agttgcacct atggacataa gagctaaatt tcctgtataa
     5941 gatataaaag atgatataga cccttgaatg ttagataata ctccttcttg aaaagttatt
     6001 ttaagatttt ttatatactc tttttctaat ttttctatag tcttttcttc agctgcatga
     6061 tactttatag tatctatgcc tcttaaactt tcgattatag tactattaag actagaacta
     6121 gcttccattt gctttaaatt tattttttta taaggtattt taaaaataaa tataagagaa
     6181 gcacttatta aagtaagaat tattactact aaaaataatg ttttattcat taaacaaaga
     6241 attattgcag atactactgt taaaactata tcaattatta atgataaagt tacagatgtt
     6301 aaaatatttt ttattacaaa agcatcacta aatcgagtta atatatcacc tacttttctt
     6361 gtttcaaaaa acttagtagg tagtttatat acatgtttaa aataacctaa caacaaaggt
     6421 atatctattt tttgagataa atataacaac atatgttgtc ttatagccga tattaaaact
     6481 tgagttactc ctaaagttat aaaacatata caaaaaataa taagttgatt ctttaatcca
     6541 taaggtaaaa tttcatcaat taaaatctta ttaaaaaaag atgttgatat acctaaaacg
     6601 gtcaatataa ctgagcttat aatactacaa ataaataatt tcttttgtgg taaaagtatt
     6661 tttataaact ttgataaagt tccttcttct ttagacctat ctaaactaaa atcattatta
     6721 ggagcaagta aaagaagtat gccatcaaac cttttaaaaa attcatttat attttccttt
     6781 tcttttccct tggctggatc tagtatgata actttattat tttttatttt atgtataata
     6841 acaaaatgac ttaatccttc atcagtaact atgtgtgcta tagctggtaa agtaaactta
     6901 ctcataaaac cggatttatc aactcgaata gctttggtat caaatcctaa tctattagca
     6961 ccattttcta aacctttaag cgttgtcccc ttaatatctg taccaagaat atctctaagt
     7021 ttagttattg taatttcttt tttataatac atacatacac tagctaaaca tgctgctgcg
     7081 caatctgtac tgtcatgttg ttttattaaa tatttattat tataaaacaa atttcaagcc
     7141 ctttcttctg aaaaaaatct tttttacttt ttaaaaagta cacttagaaa ttaacattaa
     7201 tttttaacaa aaacaagaaa
//
LOCUS       DAEVGI010000125         7187 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEVGI010000125 DAEVGI010000000
VERSION     DAEVGI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.125"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            191..1021
                     /locus_tag="K8M77_002023"
     CDS             191..1021
                     /locus_tag="K8M77_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086496.1"
                     /translation="MRKISQYLLFIPCIVLVAYFMIVPLVDVIIPTFTNTNGGMFSAY
                     KEFFTDSYMMSIFWRTIKISLVSSIICMFIGVPVSYYISRVSKKVRGFLIALTVFPIL
                     TNSVVRSFAWMSILGKNGIINSLLMKFNLIQEPLSLLYTEGAIIIGTIYIFLPLMIIS
                     LVGVMENIDNDLLEAAESLGANRLKAFFKVVFPLSLPGLIVGTVLVFTGSLTAYTTPQ
                     LLGGNKNTVLATLIYQKTMTLGDWQGAAVVATIMIVVTLIVIKGINFLASRLDKRGVS
                     "
     gene            1023..1805
                     /locus_tag="K8M77_002024"
     CDS             1023..1805
                     /locus_tag="K8M77_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086497.1"
                     /translation="MKKSKVLTTFVVLVYIFLFAPLVIIAMTSIGTENYIVFPPKGFS
                     LKWFITVFKSESFIKSMGTSFLISTVSTLSALVIGMPAAYGLSRYNFKGKDILKSFFF
                     SPLIVPGIVVGFSLFQFLLVKMNLSVYASLFIGHTLIIIPYIIRVVGSSLEGFDYSIE
                     EAAMSLGCKKSQTFFKVVLPNITSGVMAAFMLAFINSFNNVPVSIFLTGPGVSTLPTT
                     MMNYVEYNYDPTVSALSVILMILTIGIMYILEKTLGLNNFAG"
     gene            1841..2872
                     /locus_tag="K8M77_002025"
     CDS             1841..2872
                     /locus_tag="K8M77_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086498.1"
                     /translation="MDLINLQNINVSYDKKNNILENLNLKVKKGELVSLLGPSGCGKT
                     TTLRVVAGFIEPTAGKFLLGDEDYTNIPVHNRNFGLVFQSYALFPHLTVEENVAFGLK
                     MKKMSKEEIRKKVDDILKVVDMSHLVKRYPKELSGGQRQRVAIARALVIEPSLLLLDE
                     PLSNLDAKLRLKMRVEIRKLQQKLGITTLFVTHDQEECFSISDKVAVLNKGVIEQFDT
                     PENIYSNPATEFVARFVGFENFIDLIKVSDGIYKNESGINLKVNKVKDKNEVKATIRP
                     DDIKIVKEDLENTVNGVIEVRTFLGKAYQYDIKTKLGNLIVNSENDSIYEKGQNIKLQ
                     LPVDKIVIL"
     gene            2912..3958
                     /locus_tag="K8M77_002026"
     CDS             2912..3958
                     /locus_tag="K8M77_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI7086499.1"
                     /translation="MKKKILATLLTGLVLGTSLVGCGKTEGAETGKKLVVSTWGLNED
                     VLKETVFEPFAKEHGVEIVLDIGNNSERLTKMKNNPNSQIDITYLAESFAEQGVEAGI
                     FDKLDYSKIPNASEMNEKAKSTVEAGYGPAYTLNSIGIVVDPSAGIEINSWEDLWKPE
                     LKNKIAIPDITTTNGPAMVEIAAEKAGVDVKTDNGEAAFKELEALKPNVVKTYSKSSD
                     LANMFSNGEIVAAVASDFAFGTISKAKPEVINVIPESGTYLNFNTININKNSKNKDLA
                     YEFINYALSKEVQEKTAKALNESPVNKEVKLNGEETKNLTYGPVVDNAKVIDFKFVNS
                     VMDQWVNNWNRIMN"
     gene            3974..5692
                     /locus_tag="K8M77_002027"
     CDS             3974..5692
                     /locus_tag="K8M77_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBI7086500.1"
                     /translation="MQVELIIKNLKVYNSYFKKFIKSDVLINEGKFLHIGKGYEDRLW
                     SENIIDGEDKYIIPGLIDIHMHIESSMTIPREFSKAAIKHGVTTVVADPHEIANVFGV
                     RGIEEFIKFKGNLDIFYGIPSSVPSTSSSLETTGEKITHNEVKRLLEYDNIICLGEVM
                     NFKDLIEDDDSNINKIINISKEKNIPLEGHCPKIQGVDLSLYIYRGVNGDHTQQSVGS
                     LQEKIQNGMFIEMQHKSMTLENIKFLIENNLYEHFALVTDDVMADKLVKGHLDEILKE
                     AVKLGMSIENAIYASTYTPARRMNLLDRGTIAPGKLADFILLDSIEDFNIYEVYKNGE
                     MVFNRDKGLKEEFFEDKSKLDYRFYNSIELNNITKESLEVKVPNKYKNKVNCRTMKVL
                     KNTTFTEEGEVTLNVYNNILQWEKSSCALIAVFERYGKNNNISFGLVEGEIIKEGAIA
                     TTWAHDHHNLMVMGRNISDMTIAANEVINSRGGYVVSKNNEVIAKLELPIGGIISDEP
                     IEIIGEKLGEVRNAMRDLGYNHMNEIMSFSTLSLPVSPALKITDKGLIDVKKGSIVSL
                     FKKGLY"
     gene            5829..7163
                     /locus_tag="K8M77_002028"
     CDS             5829..7163
                     /locus_tag="K8M77_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBI7086501.1"
                     /translation="MSKIVTLIKNVTVITMNEHKEIIENGLVVFEKNKIVYVGTDVRT
                     EEKLKRSGYKVEVIDGEEGILMPGMINCHTHGSMVPFRSLADDCKDRLKRYLFPLEQR
                     LVDKELTYIGAKYAIAEMLLGGVTTFCDMYYFEDEVAKAAKELNMRGVLCETIVDFPS
                     PDSEKAFGGLDYSIKFIEKWKNDDLITPGIAPHAPYTNTEESLKEAYKISKKYDVPIT
                     MHLAEMDYELEECKDKYNLTPVAYLDKLGILDSNFIAAHAVLVNEEDIEILRKNNVNI
                     SHNIGANSKGAKGIAPILKMREKGINIGLGTDGPMSGNTLDILSQMSQVGKIHKLFNK
                     DRTLLPSIDLIEMGTIGGAKVLGIDKEVGSIEIGKKADLTLIETKSVNMQPIYDYYAT
                     IVYSANSSNVELVVVDGKIVVKDKKLVSASFSEVRKDLLSLTEKIKKISKEL"
BASE COUNT         2859 a          734 c         1336 g         2258 t
ORIGIN      
        1 attcgtgatt tttattgaaa tatgaataaa tttttaattt gattaattta aaggtattaa
       61 aataatataa tttacgaata ttattttata ttatattgac aatatacgca ataaaaagta
      121 gaataaaatt aaatttgtag aaattaaaaa cagtgagtaa agatacagca cttagagacg
      181 gaggaaaaac atgaggaaaa taagccagta tttattattt ataccctgca tagtattagt
      241 agcatatttt atgatagttc cattagtaga tgtaataatt cctactttca ctaatacaaa
      301 tggtggtatg tttagtgcct ataaagaatt tttcacagat tcttatatga tgtcaatatt
      361 ttggagaacc attaagatat ctttagtatc atctataatt tgcatgttta taggtgtgcc
      421 agtttcttat tacatatcaa gagtttctaa aaaggtgaga ggttttctta tagccttaac
      481 agtctttccc atattaacaa attcagttgt tcgttcattt gcttggatga gtatcttagg
      541 aaaaaatgga ataatcaata gtttattaat gaagtttaat ttaatacaag agccattatc
      601 actactatat acagaaggag ctattataat aggtacaatt tatattttct taccacttat
      661 gattatctcc ctagtaggag ttatggaaaa tatagataat gacttattag aagcagcaga
      721 aagtttagga gctaatagat taaaagcatt ctttaaagtt gtatttccac ttagcttacc
      781 aggattaata gttggaacag ttttggtatt tacaggttcc ttaacagctt acacaacacc
      841 tcaattatta ggaggaaata aaaatacggt tttagcaact ttaatatacc aaaaaactat
      901 gacattaggt gattggcaag gggccgcagt tgtagcaaca ataatgattg tagtcacttt
      961 aatagtaata aaggggatta acttcctagc atcaagatta gataagagag gggtttctta
     1021 aagtgaagaa gagcaaagta ttaacaacct tcgttgtttt ggtttatata tttctatttg
     1081 caccgctagt aattattgcc atgactagta taggaacaga aaattatata gtatttcctc
     1141 caaaaggatt tagtttgaaa tggtttatta cagtatttaa atctgagtca tttataaaaa
     1201 gtatggggac aagcttctta atatctacag tatctacatt atcagctttg gttataggaa
     1261 tgccggcggc ttatggatta agtagatata acttcaaagg aaaagatatt cttaagagtt
     1321 tcttcttttc gcctttaata gttcctggaa tagtagtggg attctcattg tttcagtttt
     1381 tattggtaaa gatgaactta tcagtatatg ctagtttgtt tattggacat actttaataa
     1441 taattcctta catcataaga gttgtaggat caagtttaga ggggtttgat tattccatag
     1501 aggaagcagc tatgagctta ggatgtaaaa aatcacaaac tttctttaaa gtagttttac
     1561 caaatataac atcaggggta atggcagcat ttatgttggc ttttataaat tcatttaata
     1621 atgttccagt atctatattt ctaactggac caggagtaag cacattacct acaactatga
     1681 tgaactatgt tgaatataat tatgatccaa cagtatcagc tttatcagta atcttaatga
     1741 tattaactat tggaattatg tacatattag aaaaaacatt aggattaaat aattttgcag
     1801 gatagaattt aagttaaaaa tttatttgga ggatgaaaac atggatttaa taaacctaca
     1861 gaatataaat gtttcttatg ataagaaaaa taatatattg gaaaatctaa atttaaaagt
     1921 taaaaaagga gaattagttt ctcttttagg accaagtggg tgtggaaaga caacaacatt
     1981 aagggttgta gctggattta tagaaccaac agcaggaaag tttttattag gagatgaaga
     2041 ctatacaaat atacctgttc ataatagaaa ctttggatta gtctttcaaa gctatgcatt
     2101 atttcctcac ttaacagtag aagaaaatgt agcttttgga ttaaaaatga aaaagatgag
     2161 caaagaagaa ataagaaaaa aggttgatga tatattaaaa gttgttgata tgagccattt
     2221 agtaaagagg tatccaaagg aattatctgg aggacaaaga caaagggttg ccatagcaag
     2281 agccttagta atagagccaa gtcttttatt actagatgaa cctttaagca acttagatgc
     2341 taaattaaga ttaaaaatga gagttgaaat aagaaaactt caacaaaagc ttggaataac
     2401 aacattattc gtaactcatg accaagaaga atgtttttca atttcagata aagtagcagt
     2461 tttaaataaa ggggtaatag aacaatttga tactccagaa aatatttatt caaatccagc
     2521 aacagagttt gttgcaagat ttgtaggttt tgaaaacttt atagatttaa taaaggtatc
     2581 agatggtata tacaaaaatg agagtggaat taacttaaaa gttaacaagg ttaaggataa
     2641 gaatgaagta aaagcaacta taagaccaga tgatataaag attgtaaaag aggatttaga
     2701 aaacacagta aatggagtta tagaggttag aactttctta ggaaaggcat accaatatga
     2761 tattaaaaca aaacttggga atctaattgt aaatagtgaa aatgacagca tatatgaaaa
     2821 gggacaaaat ataaaacttc aattaccagt ggataaaata gtaattttat aaaacttaaa
     2881 tataaaaaat atgtttataa aggagaatct tatgaaaaag aaaattttag caaccttatt
     2941 aacaggatta gtactaggga catctttagt tggatgtgga aaaacagaag gagcagagac
     3001 aggaaaaaaa ttagtagttt caacttgggg attaaatgaa gatgtattaa aggagacagt
     3061 atttgaacca tttgctaaag aacatggtgt ggaaatagtt ttagatatag gtaataactc
     3121 agaaagatta actaagatga aaaataatcc taactcacaa atagatataa cttatttagc
     3181 agaatctttt gcagagcaag gagtagaagc aggtatattc gataaattag attattcaaa
     3241 gattcctaat gcaagtgaga tgaatgaaaa ggctaaatct acagtagagg caggatatgg
     3301 accagcatat acattaaata gtataggaat agttgtagat ccttcagcag gaattgaaat
     3361 aaattcttgg gaagatttat ggaaaccaga acttaaaaat aaaatagcta taccagatat
     3421 tacaacaact aatggtccag caatggtaga aatagcagca gaaaaggcag gagtagatgt
     3481 taaaactgat aatggagaag ctgcatttaa agaattagaa gctttaaaac caaatgttgt
     3541 taaaacttat agtaaatctt cagatttagc taatatgttc tcaaatggtg aaatagtagc
     3601 tgctgtagca tcagactttg cttttggaac aatttcaaaa gctaaaccag aggttataaa
     3661 tgtaatacct gagtcaggaa cttacttaaa ctttaataca ataaatataa ataaaaattc
     3721 taaaaataaa gatttagctt atgaatttat taactatgca ttaagcaaag aagttcagga
     3781 gaaaactgct aaagctttaa atgaatcacc agttaataaa gaagttaaat taaatggaga
     3841 agaaactaaa aacttaacat atggaccagt ggttgataac gctaaggtaa ttgactttaa
     3901 gtttgttaat tcagtaatgg atcaatgggt taataactgg aacagaataa tgaactagtt
     3961 tgcaggtagt gaaatgcaag tagaattaat tattaaaaat ttaaaggtat ataatagtta
     4021 ttttaaaaaa ttcataaaaa gtgatgtctt aataaatgag ggaaaatttc ttcatatagg
     4081 gaaaggatat gaagatagat tatggtctga aaatataata gatggggaag acaaatatat
     4141 aattcctgga ttaatagata tacacatgca tatagagagt tctatgacta ttccaaggga
     4201 attttctaag gctgcaataa aacatggggt cactacagta gtagctgatc ctcatgaaat
     4261 tgctaatgtt tttggagtaa gaggtataga agaatttatt aaatttaagg gaaatctaga
     4321 tatattttat ggcataccta gctcagtacc ttcaacttca agttcactag agacaacagg
     4381 ggagaaaata actcacaatg aggtaaaaag attattagag tatgataata ttatttgtct
     4441 tggagaagta atgaacttta aagacttaat agaagatgat gattctaata taaataaaat
     4501 aataaatata agtaaagaaa aaaatatacc tttagaaggg cattgtccta aaatacaagg
     4561 agtagattta tcactttata tttatagagg ggttaatggg gatcatactc agcagtcagt
     4621 tggatccctt caagaaaaaa ttcaaaatgg tatgtttata gagatgcaac ataaatcaat
     4681 gacattagag aatataaaat ttttgattga aaataattta tatgagcatt ttgcattagt
     4741 aacagatgat gtaatggcag ataaattagt taaggggcat cttgatgaga ttttaaagga
     4801 agccgtaaag cttggaatgt ctatagagaa tgcaatatat gcatctacat atacgccagc
     4861 tagaagaatg aatcttttag atagaggaac aattgcacct ggaaagctag cagattttat
     4921 attgttagat agtatagaag attttaatat atatgaagtt tataaaaatg gggaaatggt
     4981 ttttaataga gataaggggt taaaagaaga gttttttgaa gataaaagca aattagatta
     5041 tagattttat aatagtatag aattaaataa tattactaaa gaaagtttag aggttaaagt
     5101 accaaataag tataaaaata aagtaaattg tagaactatg aaagtcttaa agaatactac
     5161 ttttacagaa gagggagaag taactttaaa tgtttataat aatattttac aatgggaaaa
     5221 gagtagctgt gcattaattg ctgtttttga gagatatggt aaaaataata atatatcttt
     5281 tggattagta gaaggagaaa ttataaaaga gggagcaata gcaactactt gggctcatga
     5341 tcatcataac cttatggtaa tgggaagaaa tattagtgat atgactatag cggctaatga
     5401 ggttataaat tctaggggag gatatgtagt ctcaaaaaat aatgaagtaa ttgctaaatt
     5461 agagttacca ataggtggaa taatcagtga tgaacctatt gaaatcatag gagaaaagct
     5521 aggggaagtt agaaatgcca tgagagattt aggatataat catatgaatg aaattatgtc
     5581 atttagcact ttatctttac ctgttagtcc tgcattaaag ataacagata aagggttaat
     5641 agatgttaaa aaaggtagta ttgtttcttt attcaaaaaa ggtctatatt aagtgattat
     5701 tgatttttat ttagttagct ttaacttaat tacgcaaagt aatataaaag gaaagtaagg
     5761 ggatgtatcg tagtagatat agcctcttat ttttagctat attttaggtg gattttggag
     5821 gggataatat gagtaaaata gtaactttaa taaaaaatgt tacagttatt actatgaatg
     5881 aacataaaga aattatagaa aatggtttag ttgtttttga aaaaaataaa atagtatatg
     5941 ttggaactga tgtaaggaca gaagaaaaat taaaaagaag tggatataaa gttgaggtaa
     6001 tagatggtga agagggaata ttaatgccag gtatgataaa ctgtcatact catgggtcta
     6061 tggttccttt tagaagttta gctgacgatt gtaaggatag attaaagaga tatttatttc
     6121 ccttagaaca aagattagtt gataaagaat tgacttatat aggagcaaag tatgctatag
     6181 cagaaatgtt attaggtgga gtaactacct tttgtgatat gtattatttt gaagatgagg
     6241 tagcgaaagc tgctaaagaa ttaaatatga gaggagtgct ttgtgagaca atagtagatt
     6301 ttccttcgcc agattcagaa aaggcttttg gaggtttaga ttattccata aaatttatag
     6361 aaaaatggaa aaatgatgat ttgataactc caggaatagc tcctcatgca ccttatacaa
     6421 atacagagga atcattaaag gaagcatata aaattagtaa aaaatatgat gttcctataa
     6481 ctatgcattt agcagaaatg gactatgagt tagaggaatg taaagataaa tataatctta
     6541 ccccagttgc ttatttagat aagcttggaa ttttagactc taattttata gcagcccatg
     6601 cagttttagt taatgaagaa gatatagaaa ttctaaggaa gaataatgtt aatatatctc
     6661 ataatatagg agcaaattct aaaggagcta aaggaatagc accaatatta aagatgagag
     6721 aaaaaggaat aaatattggg cttggaacag atggtcctat gagtggaaat actttagata
     6781 tattaagcca gatgtcacaa gtaggaaaaa ttcataaatt atttaataaa gatagaactt
     6841 tactaccatc aatagattta atagaaatgg gaaccatagg tggagctaaa gttttaggaa
     6901 ttgataaaga agttggatct atagagattg gtaagaaagc tgatttaact ttaatagaaa
     6961 caaaatcagt aaatatgcaa cctatatatg attattatgc aacaatagta tattctgcta
     7021 attctagcaa tgtagaatta gtagttgttg atggtaagat tgttgttaag gataaaaaat
     7081 tagtaagtgc tagcttttct gaagtaagaa aagatttact aagtttaaca gagaaaataa
     7141 agaaaatttc taaagaactt taggggttta tataataaga aagctta
//
LOCUS       DAEVGI010000126         7143 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEVGI010000126 DAEVGI010000000
VERSION     DAEVGI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.126"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            130..1167
                     /gene="citC"
                     /locus_tag="K8M77_002029"
     CDS             130..1167
                     /gene="citC"
                     /locus_tag="K8M77_002029"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBI7086502.1"
                     /translation="MSEWNYENTFEEELTFFSFNPQINKKYKFQVEEFLNKENLRFVE
                     SINFTVCVFNNKELIATGSLDGYILKCFAIRNDYRKKGISEKILRILTEKAFENGETH
                     LFIYTKPKNSKLFESLGYHQLINIPNLVSLLENKSTGIRTYISNLQKESYLQGVKVAS
                     IVMNCNPFTKGHKYLIEKASKENDVVHLFILTEDKSEISTKDRINMVKLGTKHLKNVL
                     IHEAGKYIISSATFPSYFIKEQKNITKAHAYLDLTLFCEYISKALNIKYRYVGEEPFS
                     NLANEYNQYMKEILPKYNIQVIEVKRLKEDGQAISASNVRRLLKEGNLGKVESLVPKT
                     IFDYLLNISKK"
     gene            1191..1496
                     /gene="citD"
                     /locus_tag="K8M77_002030"
     CDS             1191..1496
                     /gene="citD"
                     /locus_tag="K8M77_002030"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBI7086503.1"
                     /translation="MEIKKPALAGTLESSDCIVSVEPSRDNTIEINLTSTVKKQFGNE
                     IIRVAKETLKNLGVNSVVMEINDKGALNCVIEARIEAAVCRASEINEFDWGSYKCQD"
     gene            1484..2374
                     /gene="citE"
                     /locus_tag="K8M77_002031"
     CDS             1484..2374
                     /gene="citE"
                     /locus_tag="K8M77_002031"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBI7086504.1"
                     /translation="MPRLRRSMMFVPGSKPAMIKDCTIYGADSVMFDLEDSVSIAEKD
                     SARRLVYHALTSMDFGDTETVVRVNALDSEFGIEDLKAIVRAQPDVIRLPKTETAQDV
                     LDMEKVIASIEEEIGLPIGTTKMMAAIESALGVLNAYEIATSSKRLMGIALGAEDFVT
                     DMKTHRSPEGNELFAARSHIILASRAAKISAFDTVYSDVNNEEGFIKEATLIKQLGFD
                     GKSLINPRQIDLLHKVFEPTEKEIDKAIKIIEAAKEAEKRGSGVVSLNGKMIDKPVIA
                     RAERVLMLAKASGISYEKEV"
     gene            2378..3937
                     /gene="citF"
                     /locus_tag="K8M77_002032"
     CDS             2378..3937
                     /gene="citF"
                     /locus_tag="K8M77_002032"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBI7086505.1"
                     /translation="MGINSININIPDDILKERKTVEFKGEFSLEPKGNIQGRKITATF
                     CGKNKKLSSIAESIEKTGVKDGMTISFHHHFRAGDYIIRMVMKEIANLGIKDLNLCVS
                     SLTSAHEFLIDYIKDGTVTGIETSGIRGKLGEEISKGIMKKPVIIRSHGGRARAIEAG
                     DTKIDVAFLGVPCSDDYGNANGIHGKSQCGSLGYAIIDSKYADKVVLITDTIMPYPNV
                     PASISQQDVDYIVEVEEIGDPNGIASGATRFTKDPKELLIAKYASAVIEASGYLKDGF
                     SIQAGTGGASLAVARFLKEKMEEKNIKASFGLGGISKQFVDLHEEGFIGTLFDVQGFD
                     LKAVESIGKNNKHVEIDASLYANPHNKGCIANKLDIVILSALEVDKNFNVNVITGSDG
                     YIRGASGGHSDTAAGAKLSMIVSPLVRGRIPTVVDKVNTVITPGESIDVIVTERGVAI
                     NDKLSKNKELKERLESNKNIPILSIEELHEKALKITGTPEPIKYEDRVVGIIEYRDGS
                     IIDVIHKVKED"
     gene            3956..4468
                     /gene="citX"
                     /locus_tag="K8M77_002033"
     CDS             3956..4468
                     /gene="citX"
                     /locus_tag="K8M77_002033"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBI7086506.1"
                     /translation="MNEKNKFLMEILKGREERAFEVKKLNERFQGTIICLTLNIPGPD
                     KLDELYYYAFNEATKALEASLNIHVLKERAPLSGYEAYFVSDKDIKEIKKIALTLEEK
                     HPLGRLFDIDIFYKDLSKISREDLGEPKRKCFLCGEDAVSCGRNRKHSVYELISSIKE
                     KILQYKNNKN"
     gene            4509..5654
                     /locus_tag="K8M77_002034"
     CDS             4509..5654
                     /locus_tag="K8M77_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBI7086507.1"
                     /translation="MTKDELLKQRELAHGLISIKPNFDINNREQLSQIYTPGVSTICK
                     EVEHHPSMVKTLTSVGNSIAVITDGTAVLGLGNIGTLAGYPIVEAKALVYKDLAGVNA
                     IPLCVDQIGCNELIKTIKNISSSFSGIHLEDIKAPECFYIEDELKKTLNIPVYHDDQH
                     GTAIAVLGALYNASKVVNKDFSKLKVLILGAGASGIATAKLLLKAGIEDIILVDKNGA
                     LVSGDETLNDPQKEMAKITNKELKKGTLEEVIKGRDVFIGLSEGNLVTKEMVESMNED
                     PIIFALANPTPEIKPEIAKEAGARVIATGGPSYPNQINNILVFPGLFKGLLEAKATDV
                     TYGVMIAVSKKLASLVENPTAEKIIPGVFDGDIVKSVSETVVKNIEK"
     gene            5675..7015
                     /locus_tag="K8M77_002035"
     CDS             5675..7015
                     /locus_tag="K8M77_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBI7086508.1"
                     /translation="MELQNKKVDGKEESLLKKFFNINLFGVPMLLFLVGAIIIILGIS
                     TNSLPKDMVGSIFLIFTCGIVLGKIGDSIPIWKDYLGGGAILAFLVTSYAVYIGLIPT
                     TYVKDIKELFDSGFLELYISIMICGSLLAIDRKFLAKTVGGFIPMVIIATLTAALGAV
                     IGGLITGVSPKEVILNYALPIMGGGNGAGAIPMSQIWGQVTGKDPKIWYSSAMAILTI
                     ANIIAILAGAILNGIGNKKPKLTGNGELIKGVKNAESTKSNFKATFEDGAAGLFITLA
                     FYCLGNLFGKGFFPTIGGVFIHPFAYMVVFVLIASGFNLVPERIRVGAKQVQKFMVGN
                     LFYVLIAGVGIAMVDFGALLKAFNLTTVIISLGAVIGAILGPWITSKIFGFYPIEASI
                     AAGLCHVNRGGSGDLEILGAAKRMNLMAYAQIATRLGGAIILVLAGFLFSLWLK"
BASE COUNT         2748 a          801 c         1350 g         2244 t
ORIGIN      
        1 aaaaaccgtg aaaacgttat aattttcgtg aatgcagaaa atctattgta ctttgaaaag
       61 agtgtggtaa aataaaattg taagtaacaa atgtttaaaa aataaacaaa tgttaaaagg
      121 agatatgaaa tgtcagaatg gaattatgaa aatacttttg aagaggaatt aactttcttt
      181 tcctttaatc cacaaataaa taagaaatat aaatttcaag ttgaagaatt tttaaataaa
      241 gaaaatttga gatttgtaga aagcattaac tttacagttt gtgtatttaa taataaagaa
      301 cttatagcta cgggatcctt agatggatat atactaaaat gttttgctat tagaaatgat
      361 tatagaaaaa aaggcattag tgaaaagata ttaagaatat taacagaaaa agcttttgaa
      421 aatggagaaa cacatctttt tatatacaca aaaccaaaga actcaaagct ttttgaaagt
      481 ctaggttatc accaattaat aaatattcct aatttggttt ctttattaga aaataaatca
      541 acaggaatta gaacttatat aagtaattta caaaaagaat cttatctaca aggagtaaag
      601 gtagcttcaa tagttatgaa ttgtaatcca tttacaaagg gacataagta tttaattgaa
      661 aaagcttcaa aggaaaatga tgttgttcat ttatttatat taacagaaga taaatcagaa
      721 atttctacta aagatagaat taacatggtt aaacttggaa ctaaacatct gaaaaatgtt
      781 ttaatacatg aggcgggaaa atatattatt tcaagtgcca catttccttc atactttata
      841 aaagaacaaa aaaacattac aaaagctcat gcttatttag atttaacttt attttgtgag
      901 tatatatcaa aagcattaaa tattaaatac agatatgtag gagaagaacc tttttcaaat
      961 ttagcaaatg aatacaatca atatatgaaa gagatacttc ctaaatataa tatacaagtc
     1021 atagaagtta aaagattaaa agaagatgga caagcaattt cagcttctaa tgtaagaaga
     1081 ttattgaaag aaggaaatct aggaaaagta gagagtttag ttcctaaaac aatatttgat
     1141 tatttattaa acataagtaa aaaataataa acattttgga ggggtttcat atggaaatta
     1201 aaaaaccagc tttagctgga acattagaat caagtgattg tatagtttca gtagagcctt
     1261 caagggataa cactattgaa attaatttaa caagtactgt aaaaaagcaa ttcggaaatg
     1321 aaattataag agttgcaaag gaaaccttaa aaaacttagg agttaacagt gttgttatgg
     1381 agataaatga taagggagcc ttaaattgtg ttattgaagc acgtatagaa gcggcagtat
     1441 gtagagcttc agaaataaat gaatttgatt gggggagtta taaatgccaa gattaagaag
     1501 atcaatgatg tttgtacctg gaagtaagcc tgctatgata aaagattgta ctatatatgg
     1561 tgctgattct gtaatgtttg acttagaaga ttcagtttct atagcagaaa aagattctgc
     1621 aagaagatta gtttatcatg ctttaacttc tatggatttt ggtgatacag aaacagtagt
     1681 ccgtgttaac gctttagatt cagagtttgg tattgaagat ttaaaagcaa ttgtaagagc
     1741 acaaccagat gttatacgtt tacctaaaac tgaaactgct caagatgttt tagatatgga
     1801 gaaggttatt gcatctatag aagaagaaat aggtcttcct attggaacaa ctaaaatgat
     1861 ggctgctata gaatcagcct taggtgtttt aaacgcttat gaaatagcaa cctcttcaaa
     1921 acgtttaatg ggaattgcct taggtgctga agattttgta actgatatga aaactcatag
     1981 atctcctgaa ggtaatgaac tttttgcagc acgtagtcat ataatattag cttcaagagc
     2041 ggctaaaatt tcagcttttg atacagttta ttctgatgta aataatgaag agggctttat
     2101 taaggaagca acattaataa aacaactagg atttgatgga aaatccttaa taaacccaag
     2161 acaaatagac ttattacata aagtttttga acctactgaa aaagaaattg ataaggcaat
     2221 aaaaattata gaagctgcta aagaagcaga aaaacgtggc tcaggcgttg tatctcttaa
     2281 tgggaaaatg attgataagc cagtaatagc aagggcagaa agagttctta tgttagctaa
     2341 ggcttcagga atatcatatg aaaaggaagt gtaatttatg ggaatcaata gtatcaatat
     2401 aaatatacca gatgacatat taaaagagag aaaaactgta gaatttaaag gagaattctc
     2461 attagaacct aaggggaata ttcaaggaag aaaaataaca gcaacttttt gtgggaaaaa
     2521 taaaaagtta tcatctatag ctgaatctat agaaaaaaca ggagttaaag atggtatgac
     2581 tatatctttt catcatcact tccgtgctgg agattatatc attcgtatgg ttatgaagga
     2641 aattgctaat cttggaatta aggatttaaa tctttgcgta agttctttaa caagtgctca
     2701 tgaattttta atagattata taaaagatgg cacagtaaca ggaatagaaa caagtgggat
     2761 tcgtggaaaa ctaggagaag aaatttcaaa aggaataatg aaaaaaccag taattatccg
     2821 ttctcatggt ggacgtgctc gtgcaattga ggctggtgat acaaagatag atgtagcctt
     2881 tttaggtgtt ccttgttcag atgattatgg aaatgctaat ggaatacatg gaaaatctca
     2941 atgtggttct ttaggatatg ctattattga ttctaaatat gcagacaagg tagttcttat
     3001 tacagatact ataatgccat atcctaatgt accagcaagt atctctcaac aggatgtaga
     3061 ttacattgtg gaagttgaag aaataggtga tccaaatggt attgcttcag gggctacaag
     3121 atttacaaag gatcctaaag agttattaat agcaaaatat gcttccgcag taattgaagc
     3181 ttctggatat ttaaaagatg gtttttcaat tcaagcaggt acaggtggag cttcattagc
     3241 agtagctcgt ttcctaaaag agaaaatgga agaaaaaaat ataaaggcaa gctttggcct
     3301 tggtggaata agtaaacaat ttgttgattt acatgaagaa ggatttatag gaacattatt
     3361 tgatgttcaa ggatttgatt taaaagctgt tgaatctatt ggtaaaaata ataaacatgt
     3421 agaaatagat gcttcgcttt atgcaaatcc acataataaa ggatgtattg ctaataaatt
     3481 agacatagta atattaagtg ctttagaagt ggataaaaac ttcaacgtaa atgttataac
     3541 tggttctgat ggttatataa gaggggcatc aggtggacac tcagatacgg ctgctggtgc
     3601 taaattatca atgatagttt ctcctttagt tcgtggtaga ataccaacag tagttgataa
     3661 ggttaatact gtaataacac ctggtgaatc aatagatgtc attgtaacag aacgtggtgt
     3721 tgctataaat gataagctct ctaagaataa agagttaaaa gaaagattag aatcaaataa
     3781 aaatattcct atattatcaa tagaagagct ccatgaaaaa gctttaaaaa ttacagggac
     3841 tccagaacct ataaaatatg aagatagagt tgttggaatt attgaatacc gtgatggttc
     3901 aataatagat gttatacaca aggttaaaga ggattaaaaa tcaagaggat aaaaaatgaa
     3961 cgaaaagaat aaatttttga tggaaatatt aaaaggtaga gaagagagag catttgaagt
     4021 aaaaaaacta aatgaaagat ttcagggaac tattatatgt ttaacattaa atattcctgg
     4081 tccagataaa ttagatgaac tttattatta tgcatttaat gaagccacta aggcattgga
     4141 ggcttcatta aatatacatg ttttaaaaga aagagctcct ctttctggat atgaagctta
     4201 ttttgtttca gataaggata taaaagaaat aaaaaaaata gctttaactt tagaggaaaa
     4261 acatccttta ggaaggctat ttgatataga tatattctat aaagacttat ctaaaataag
     4321 cagggaagat ttaggagagc ctaaaagaaa atgtttttta tgtggagaag atgcagtatc
     4381 ctgtggcaga aataggaaac attcagttta cgaacttatt tcaagcataa aagaaaaaat
     4441 acttcagtat aaaaacaata aaaattaatt agtataaaat atggtatagt ttgaaaagag
     4501 gtaataatat gactaaggac gaattattaa aacaaagaga attagctcat ggattaataa
     4561 gtataaaacc taattttgat ataaataata gagaacaatt atcacaaata tatactcctg
     4621 gagtatcaac tatttgtaaa gaggttgagc atcatcctag tatggtgaaa acactaactt
     4681 ctgttggaaa ttcaatagct gttataacag atggtactgc ggttttaggt cttgggaata
     4741 taggtaccct tgcaggatat cctatagtag aggctaaagc tttagtttat aaagatctag
     4801 ctggtgtaaa cgctatccca ttatgcgttg atcaaatagg atgcaatgaa ttaattaaaa
     4861 caataaaaaa tatatcttca agttttagtg gaattcatct tgaagatata aaggcaccgg
     4921 aatgctttta tatagaagat gaacttaaaa aaactttaaa tattccagtt tatcatgatg
     4981 accaacatgg tactgccatt gctgttttag gggctcttta taatgcatct aaggtagtta
     5041 ataaggattt ttcaaagtta aaggtattaa ttttaggggc aggagcttca ggaattgcaa
     5101 cagcaaaatt attattaaag gctggaatag aagatattat attagttgat aagaatggag
     5161 ctttagttag tggtgatgaa actcttaatg atcctcaaaa agaaatggct aaaataacaa
     5221 ataaagaatt aaaaaaagga actttggaag aagtaattaa gggaagagat gtatttatag
     5281 gtttatcaga agggaatctt gtaactaagg aaatggtaga aagtatgaat gaggatccta
     5341 taatattcgc tttagctaat ccaacgccag agataaaacc tgaaattgca aaggaagctg
     5401 gtgcaagggt tattgcaaca ggtggacctt cttatccaaa tcagattaat aatatattgg
     5461 ttttcccagg actatttaaa ggattattag aagctaaggc aactgatgta acttatggtg
     5521 taatgatagc agttagtaaa aaattagctt ccttagttga aaatccaact gctgaaaaaa
     5581 taatacctgg agtatttgat ggtgatatag ttaagtctgt ttctgaaact gtggtaaaaa
     5641 atattgagaa gtagttaatt taaggggagt ttatatggaa ttacaaaata aaaaggtaga
     5701 tggtaaagaa gaaagtttgc ttaaaaagtt ttttaatata aatctttttg gagtgccaat
     5761 gttattgttt ttggttggag caataatcat aatattaggt atatcaacaa attcattacc
     5821 aaaggatatg gttggaagta tattcttaat atttacttgt ggtattgttt taggtaaaat
     5881 aggtgattca atacctatct ggaaagatta tttaggtgga ggtgctattt tagcattctt
     5941 agtaacttca tatgcagttt acataggatt aattcctacc acttatgtaa aggatatcaa
     6001 agaacttttt gatagtggat tcttagagtt atatatttca ataatgattt gtggttcttt
     6061 attagcaata gatagaaaat tcttagcaaa aactgttggt ggatttatac ctatggtaat
     6121 tattgcaact ttaactgctg ccttaggcgc tgtaatagga ggattaatta caggagtttc
     6181 tccaaaagaa gttatattaa attatgcact tcctattatg ggtggtggaa atggagcagg
     6241 tgctattcct atgagccaaa tttggggaca agtaacagga aaagatccaa agatatggta
     6301 ttcatcagca atggcaatat taacaattgc aaacataata gcaatattag caggtgctat
     6361 tttaaatggt ataggtaata aaaaacctaa gcttacaggg aatggagaat tgattaaagg
     6421 agtaaaaaat gcagaatcta caaaatctaa ttttaaagca acctttgaag atggcgcagc
     6481 aggtctattt ataacacttg ctttctactg tttaggaaat ctgtttggaa aaggattctt
     6541 cccaacaata ggtggagttt tcattcatcc atttgcttac atggttgtat ttgtattaat
     6601 agctagtgga tttaacttag ttccagaaag aattagagtt ggtgcaaaac aagttcaaaa
     6661 atttatggtt ggtaatttat tctacgtttt aatagcagga gttggtatag caatggttga
     6721 ttttggagct ttattaaagg cattcaattt aacaactgtt ataatatctt taggtgcagt
     6781 tataggagct atattaggtc cttggataac ttcaaaaata tttggcttct atcctataga
     6841 agcttccata gcagctggat tatgccatgt taatagggga ggttctggag acttagaaat
     6901 attaggagct gctaagagaa tgaatttaat ggcatacgct caaatagcaa ctagacttgg
     6961 tggagcaatt atattagttt tagcaggatt cctatttagt ttatggttga aatgatttga
     7021 ataaatacat atataaatgt caattaagag agattttatt taatttaaga aataaagtct
     7081 ctcttatttt atttgaaaat tgtaatttga attaaaaata aatgttaatt tggaaataat
     7141 tgt
//
LOCUS       DAEVGI010000127         7091 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEVGI010000127 DAEVGI010000000
VERSION     DAEVGI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.127"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            68..1333
                     /locus_tag="K8M77_002036"
     CDS             68..1333
                     /locus_tag="K8M77_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBI7086509.1"
                     /translation="MKKLGLAFQIILGLILGIIIGAIFYGNPVVTSYLQPFGDIFIRL
                     IKMIVIPIVFSSLVVGVAGVGDVKKLGKIGGKTILYFEIVTTFAIIIGLVIANLFHPG
                     SGVNISTLATTNIDKYMSTAQAASSHGFMDTFINIVPTNIFESLAKGDLLPIIFFSVM
                     FGLGVAAIGEKGKPVLSLCQGIADSMFWITNQIMKLAPLGVFGLIGVTVSKFGLASLI
                     PLGKLIITVYGAMFFFVFFVLGFIAKMAGTSIISLMKLLKDELILAYTTASSEAVLPK
                     LMEKMERFGCPKAITSFVIPTGYSFNLDGSTLYQSIAALFIAQIYGIHLPLSAQINLV
                     LVLMLTSKGMAGVPGASFVVLLATVGSLGIPVAGVAFIAGIDRIVDMARTLVNVLGNS
                     LAVVVISKWEKEFNAEEGEKYIKSVSEIA"
     gene            complement(1419..1535)
                     /locus_tag="K8M77_002037"
                     /pseudo
     CDS             complement(1419..1535)
                     /locus_tag="K8M77_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460070.1"
                     /note="cleaves off formyl group from N-terminal methionine
                     residues of newly synthesized proteins; binds iron(2+);
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            1724..2923
                     /locus_tag="K8M77_002038"
     CDS             1724..2923
                     /locus_tag="K8M77_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBI7086510.1"
                     /translation="MSFCCKDLFQLKYFSKAKLLAGESGLNKQISLPYVRTTESISNW
                     LYGGELLFVIYKNNDENLALLLEECIQKKLAGIVILIDDKHIDKITDHMIDRANNEAL
                     PLFVMPWDIKLIDIIQEIFLKIEQKREESKNGKHFLESLLFSQDQLSDDINALAEFYN
                     IKLRPFHYICILKMKKTSNPPYDTENISKHIANSLDDGLKEKNYTLIHMEYANHLLSL
                     VFANNYEEAKKSLEYIESSFNLMCSKYSENIEMNLSFSRIRETNSDIKASYKEAFKAL
                     SMIDMYDNDSKVIKYNELGIIRLFIELAEIKDVYQYCYENIGPILEYDKSHGMNLLET
                     LKCYFKNNRHLLKTSQELFIHRNTLLYRLSTIKNLLQRDLDDAMTDLELFNSILIFEF
                     LNLQNKL"
     gene            complement(3033..3959)
                     /locus_tag="K8M77_002039"
     CDS             complement(3033..3959)
                     /locus_tag="K8M77_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBI7086511.1"
                     /translation="MKLKGIILTMLSSITFGFAFTLGPMTYGLEGSNPVTLTFLRNFL
                     SLPFLLVIILLLKIDLRVTKKQLRDLIILGFVGNAITTLMLNMAFACIDVGIVTPIHF
                     TYPIFVTLGCVIFFHEKLSKQKVFALIIAMSGIGCFFITALNSASFGKSTLLGLILAV
                     ASGIFYAFYIIFMDKSGLKSEPPFKITFYVAIASTVGMFIYGMLTKELVLSSLTHKAW
                     GLSAIFAFLCTVVALSLLQVGIKYIGASEAAVISTFEPITSVIFGAILLGEEITLMKI
                     IACSLIFAGVLILSFAKEKTKSNIKIKESVTN"
     gene            complement(4008..4613)
                     /locus_tag="K8M77_002040"
                     /pseudo
     CDS             complement(4008..4613)
                     /locus_tag="K8M77_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
     gene            4861..6123
                     /gene="codA"
                     /locus_tag="K8M77_002041"
     CDS             4861..6123
                     /gene="codA"
                     /locus_tag="K8M77_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HBI7086512.1"
                     /translation="MKAILFKNARLKGNETLVDLLVENGVYKEIGPNLSEKYKDVETY
                     DLKGDLVVPPYVDPHIHLDYVYTARMPGANNGTGTLFEGIQRWSETKGNMTIDEIKER
                     ARIALKKEILYGTQYMRTHVDVTDPKFTGLKAIMELKEEYKDIIDIQIIAFPQEGMYS
                     YKGGDELVEEALKMGADVVGAIPHFEFTREMGEKSVKKTVELAMKYNKLIDVHCDETD
                     DDQSRFVELLAAEAYLNGIGELTTASHTCAMGSYNNAYAFKLFKLLKLSKMNFISCPT
                     ENIHLQGRYDTYPKRRGLTRVKELNDAGINVCFAQDSISDPWYPLGNGNLMNILDAGI
                     HICHMMSVDEINNALDLITTNGAKTLHIQDKYGIEVGKDANFIVLNAKNEFDAILERV
                     GVNCSVRRGEFLFKREPEVIDTKITLLK"
     gene            complement(6287..6781)
                     /locus_tag="K8M77_002042"
     CDS             complement(6287..6781)
                     /locus_tag="K8M77_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBI7086513.1"
                     /translation="MNYCFQCGTKLINKQCNNEGEVPYCNKCEMFRFPTFSSAISAIV
                     LNPDKNKVLLIQQYERKDNILVAGYINKGENAEQTLVREIKEETGLNVKDYQYMKSSY
                     YEKTNTLMCNFICVVDSEDLSQINEEVDKAEWFSFEEALKNIKSKSLAEEFLIYAFKK
                     MNYI"
BASE COUNT         2586 a          964 c          965 g         2576 t
ORIGIN      
        1 ccagttttct tgcatatatt aaatgaaaat aatttttaat ttataatata aggaggatat
       61 taaacaaatg aaaaaattag gactagcatt tcaaattata cttggcctta tactaggtat
      121 tataatcggt gctatttttt atggaaatcc agttgtcact tcatatttac aaccatttgg
      181 agatattttt ataagattaa ttaaaatgat tgtaattcct atcgtctttt catcactagt
      241 tgttggtgtt gctggagttg gagatgttaa aaaattaggc aaaatcggcg gaaaaactat
      301 tctttatttt gagattgtaa ctacattcgc tattataata ggtttagtta tagctaattt
      361 atttcatcct ggaagcggag taaatattag tactcttgca actactaata ttgataaata
      421 tatgagtaca gcacaagctg catctagcca tggatttatg gatacattta taaatattgt
      481 tccaactaat atttttgaat cccttgcaaa aggagatttg cttccgatta ttttcttttc
      541 agttatgttc ggattaggtg tagctgcaat tggagaaaaa gggaaaccag ttctttcact
      601 atgtcaaggt attgctgact caatgttttg gattactaat caaattatga aacttgcgcc
      661 acttggcgta tttggattaa taggtgtaac tgtttctaaa tttggattag cttcattaat
      721 tcctttagga aagttaataa ttactgttta tggcgccatg ttcttctttg tattttttgt
      781 tcttggcttt attgcaaaaa tggctggaac aagcattata tcacttatga aacttttaaa
      841 agatgaactt attttagctt atactacagc aagttctgaa gccgttttac caaaacttat
      901 ggaaaagatg gagaggtttg gctgtcctaa ggcaattaca tcttttgtta ttccaacagg
      961 atattcattt aacttagatg gatctacttt atatcaatct attgcagcac tttttattgc
     1021 tcaaatatat ggaattcact tacctctttc tgctcaaatt aatttagtgc ttgtattaat
     1081 gcttacttca aaaggtatgg ctggagttcc tggtgcatct tttgtagtac ttttagcaac
     1141 tgttggttct ttgggaattc cagtagcagg ggttgcattt attgctggta tagatcgtat
     1201 agttgatatg gcgagaactc ttgttaatgt acttggaaat tccttagctg ttgttgttat
     1261 atctaaatgg gaaaaggaat ttaatgctga agaaggagaa aaatatatta aatcagttag
     1321 tgaaatagca taattttaat ttactaaatt atgattttat ttaattataa aaatagtctt
     1381 agataattta tataaatatc taagactatt ttttatattt ctttattcac tcttaaggta
     1441 tcgactaaat catcaataat tcctatatct tctttagttg caagttctga tttttgtcct
     1501 aaaaataata catctttaac aattggtctt atcatattta ttccaacctt tctaatttat
     1561 cgattttaat aaactctaaa attaataata ttttaaaaaa atcacactta aaatataaat
     1621 gtaataaaca aatatgtaaa tatattaatt aatatttaat atatttataa aaaatggtaa
     1681 aataaataag aaagattgaa atgaaattaa aaggagtgac tttatgagtt tttgttgcaa
     1741 agatttattt caacttaaat atttttcaaa agcaaaattg ttagctggag aaagtggact
     1801 aaataaacaa atttctttac cctatgtaag gacaacagaa tctatttcaa attggcttta
     1861 tggaggagag ttactctttg tcatttataa aaacaatgat gaaaacctag cacttttact
     1921 agaagaatgt attcaaaaga aacttgcagg tattgttatt ttaattgatg ataaacatat
     1981 agataaaata acagatcaca tgattgatag agcaaataat gaggctcttc ccctatttgt
     2041 aatgccttgg gatataaaac taattgatat tatacaagaa atttttctaa aaatagaaca
     2101 aaaaagagaa gaatcaaaaa atggtaaaca ttttcttgaa tcacttcttt tttcacaaga
     2161 tcaattaagt gatgatataa acgctttagc agaattttat aatataaagt taagaccttt
     2221 ccattatatt tgcatactta aaatgaaaaa aacctcaaat ccaccttatg atacagagaa
     2281 tattagcaag catatagcaa attccttaga tgatggatta aaagaaaaaa attatacttt
     2341 aatccatatg gagtatgcaa atcatttatt gagtttggta tttgctaata attatgaaga
     2401 agcaaaaaaa tccttagaat atattgaatc tagttttaat cttatgtgct ctaaatattc
     2461 agaaaatatt gaaatgaact taagctttag ccgtataagg gaaactaatt ctgacattaa
     2521 agcaagttat aaagaggcat ttaaagcttt gtccatgatt gatatgtatg ataacgattc
     2581 aaaagttatt aaatataacg aactaggtat tataagattg tttattgaat tagctgaaat
     2641 taaggatgtt tatcaatatt gttatgaaaa cataggtcct attttagaat atgataaaag
     2701 tcatggaatg aatttattag aaactttaaa atgctatttt aaaaataata gacatttact
     2761 aaaaacttcc caagaacttt ttattcatag gaatacttta ctttatagat tatccacaat
     2821 aaaaaatctt ttacaaaggg atttagatga tgctatgact gacttagagc tttttaatag
     2881 catattgatt tttgaatttc ttaatttaca aaacaaatta taaatgtgga ctacccttta
     2941 aataaagatt aataattata tatttaaaat attaaaatgg actatataaa aattctaaaa
     3001 acaattttta tatagtccat agtttattgc aattaattgg taactgattc ttttatttta
     3061 atattagatt ttgtcttttc ttttgcaaag gacagtatta atactcctgc aaatattaat
     3121 gaacatgcaa ttattttcat taatgttatt tcttcaccta aaagtattgc tccaaagata
     3181 acacttgtta taggttcaaa tgtacttata acagcagctt cactagcacc aatatatttt
     3241 atacccactt gtaataatga taatgctaca actgtacata agaaagcaaa tattgctgat
     3301 aatccccatg ctttatgtgt caatgaagat aatacaagtt cctttgtaag cattccataa
     3361 ataaacatac ctactgttga agctatcgct acatagaatg ttattttaaa aggtggctca
     3421 cttttcaaac cacttttatc cataaatata atataaaatg catagaaaat tccagatgct
     3481 acggctaaaa ttaatcctaa caatgtactt tttccaaaag atgctgaatt taacgctgta
     3541 ataaagaaac atcctatacc tgacatagca ataattaatg caaagacttt ttgtttactt
     3601 aatttttcgt ggaagaatat tacacagcct aatgttacaa atattgggta agtaaaatgt
     3661 ataggtgtaa ctattccaac atctatacaa gcaaatgcca tatttaacat taaagttgtt
     3721 attgcatttc caacaaaacc aagtattatt agatctctta gttgtttctt tgttactctt
     3781 aaatctattt ttaaaagtaa aataattact aataaaaatg gcaaacttaa aaaatttctt
     3841 aaaaaagtta atgtcacagg gttgcttcct tcaagaccat aggtcattgg cccaagtgta
     3901 aaggcaaaac caaaggtaat tgaagatagc atagttaaaa taattccttt taatttcata
     3961 atatccccta ctttttatac ttaaatttat caaatactaa tttaatatta tttaaaagct
     4021 ttgtcaataa tcttgataat gtttgaaata acatcatcaa ctattatttt tcctctttca
     4081 gctgatgaag atttagctga tgcaagtatt ccagtttcag gaacaatatc tttttctatt
     4141 ggatatctat agtaagttgc agggctagca ttttctgtat ccattatttt atcttctcta
     4201 acaagttctg gtgcaaaata catcattaat gatgtttctg taacagcagc atgttctaaa
     4261 gcccaacctg ggaatggtac ttcatcaaat accttatcaa ttacgtcagg tgacattgga
     4321 tcccaccagt tagtaagaag taattgtact ttatctccat attttgcaga acataaatcc
     4381 atagcttcta ctatgaatgc ttcattttca aagtgagcac ttaatataaa tatttttgta
     4441 aatccatcac gaacaaattc atctattata tccattacta aagcttgtaa tgtagctcca
     4501 tttaaatcta tagttcctgg gaataatgga ccaccaccac ttaatggttt tgatttgtat
     4561 ccatatgata aagccggagc aactacccca tttatttttt cagcaattct ataatacaat
     4621 gagtattttt tggtgacaat gtactaaaaa tacaaatcta tttaccaaaa tatgttcttt
     4681 atgcgcacag taatattaaa ctattcttgt atattttata caatgactaa ataagtcttt
     4741 catgctaaac tttctttatt gacaagtcat tactaaaaat aatagaaatt ttaatttatt
     4801 aataatgata taagtaaatt tttttagtaa aataccaaaa atagaaggga gattatttaa
     4861 atgaaagcta ttttatttaa aaatgctaga ttaaaaggaa atgaaacact agttgattta
     4921 cttgttgaaa atggagttta taaggaaata ggtccaaatc tttctgaaaa atataaagat
     4981 gtagaaactt atgatttaaa aggagatctt gttgtaccac cttatgttga tccacatatt
     5041 catttagact atgtatatac agctcgtatg cctggtgcaa ataatggaac aggaactctt
     5101 tttgaaggaa ttcaaagatg gtctgaaaca aaaggaaaca tgacaataga tgaaattaaa
     5161 gaacgtgcaa gaatagctct taaaaaagag attttatatg gtactcaata tatgagaaca
     5221 catgttgacg taacagatcc taagtttaca ggattaaaag ctattatgga attaaaagaa
     5281 gaatataaag atattataga tattcaaatc atagctttcc cacaagaagg aatgtattct
     5341 tataaaggtg gagatgaatt agtagaagaa gctttaaaaa tgggtgctga tgtagttggt
     5401 gctattcctc actttgaatt cacaagagaa atgggagaaa aatcagttaa gaaaactgta
     5461 gagcttgcaa tgaaatataa taaattaatt gacgttcact gtgatgaaac tgatgatgac
     5521 caatcaagat ttgttgaatt attagcagca gaagcttact taaatggaat tggagaactt
     5581 acaactgcaa gccatacttg tgctatgggt tcatataata atgcttatgc atttaaatta
     5641 ttcaaacttt taaaattatc aaaaatgaac ttcatatcat gtccaacaga aaatattcac
     5701 ttacaaggaa gatatgacac ttatccaaag agaagaggtc ttacaagagt taaggaatta
     5761 aatgatgcag gaattaatgt ttgttttgct caagactcaa tttcagaccc atggtaccca
     5821 ttaggaaatg gtaacctaat gaatatctta gatgctggta ttcatatatg ccatatgatg
     5881 tctgttgatg aaattaataa tgccttagat ttaattacaa caaatggtgc taaaactctt
     5941 catatacaag ataaatatgg tatagaagta ggaaaagatg ctaatttcat agttttaaat
     6001 gctaaaaatg aatttgatgc aatccttgaa agagttggag ttaactgctc tgtaagaaga
     6061 ggagaattcc tatttaagag agaacctgaa gtaatagaca caaaaataac tctattaaaa
     6121 tagttaccat ataaattaaa gattacaaac atattccgag aatctagata ttctcttcat
     6181 attgtttgta atctttataa aaaattattt tgatatatac tctcattttt tctatatatt
     6241 ctttagaatt ctctaactca gcggctttct taatgttatt taaatattat atgtaattca
     6301 tctttttgaa ggcatatatt aaaaattcct cagctaaact tttgctttta atatttttta
     6361 atgcctcttc aaaagaaaac cattctgctt tatcaacttc ctcattaatt tgacttaaat
     6421 cttcactatc taccacacaa ataaaattac acattaatgt attagtcttt tcataataac
     6481 tactcttcat atattgataa tctttaacat taagacctgt ttcttctttt atttctctta
     6541 ctaaggtctg ttctgcattt tcacctttat taatgtatcc tgctacaagt atattatctt
     6601 ttctttcata ctgttgtata agtaacactt tatttttatc aggatttaaa acaattgcac
     6661 ttatagctga actaaatgtt gggaaacgaa acatttcaca cttattacaa tatggaacct
     6721 ccccctcatt attacattgt ttatttatta atttagtacc acactgaaaa caataattca
     6781 tagtatttac tccttatatc tagtatatag attatactag tttatttatc tttagcctct
     6841 tgttccccac tacagtgaga atttaccttt tattatattt cttttctata gcttagtcaa
     6901 aaatgtatag tataaatact ataaatcaaa tattaaatat attttctcat attattttta
     6961 acaagatctt ctctaaagaa aatatcaatt tatatgtccc tagttaaaaa tattttgtta
     7021 aaatagtata taatattata tattcaaatt cttattaata caaaaatttc aatattatag
     7081 aagttaattg t
//
LOCUS       DAEVGI010000128         7061 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEVGI010000128 DAEVGI010000000
VERSION     DAEVGI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7061
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.128"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(21..449)
                     /locus_tag="K8M77_002043"
     CDS             complement(21..449)
                     /locus_tag="K8M77_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086514.1"
                     /translation="MDNCKYNYKYNCECNLDKYEDNCNKCRKLEKIAEELTRESAELN
                     EAVEEKLEGVKYIQREIDELEERLECLRKKQKSLLCEAMELDEELDKTIFEAFKHLFA
                     TVECYKKCKNNERPERPEKPEKPEKPGRPGCGCKYCYKDR"
     gene            complement(629..1906)
                     /gene="serS"
                     /locus_tag="K8M77_002044"
     CDS             complement(629..1906)
                     /gene="serS"
                     /locus_tag="K8M77_002044"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBI7086515.1"
                     /translation="MLDLKKLRSNTEEVKKALSNRGEDFDVNVIDEVIALDEERRKIL
                     VDVEALKKQRNEVSAEIPKRKKAGEDVTEVMAEMREIGDKIKADDAKVAELNDKINYI
                     MLRIPNIPNPAVPEGETDEDNVEIKRWGEPTKFNFEPKAHWDLGTDLDLLDFERGGKV
                     AGSRFTVYKGMGARLERSIINYFLDKHTFENGYTEVLPPYMVNRDSMTGTGQLPKFEE
                     DAFKVENNGYFLIPTAEVPVTNMYRNETLEGDKLPIKHAAYSACFRAEAGSAGRDTRG
                     LIRQHQFNKVELVKFCKPEQSYEELDKLVQDAESVLQGLGLPYRIVRICKGDLGFTAA
                     LKYDIEVWMPSYNRYVEISSCSNFEDFQARRANIKYKNSPKEKPQFVHTLNGSGVAIG
                     RTVAAILENYQQEDGSVVIPEALKEYMRCDLLK"
     misc_binding    complement(1993..2200)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2258..2491)
                     /locus_tag="K8M77_002045"
     CDS             complement(2258..2491)
                     /locus_tag="K8M77_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HBI7086516.1"
                     /translation="MHKDIFTSVVRVRGSKKYNVVPVKSNKPVEISKWIDFSNVLSRL
                     YVGVPTKSGNVVCKNIMNTGVDIICTKNLPKDS"
     gene            complement(2504..3898)
                     /locus_tag="K8M77_002046"
     CDS             complement(2504..3898)
                     /locus_tag="K8M77_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7086517.1"
                     /translation="MDYDVLILGGGIIGCSVAYELSKYNLNIALIEKDFDIADDVSFV
                     NTAIVYDGSECKDNLMSYLENMGNMMLPEITKKLNVPFKRVGHLTIADNDETVKIIEE
                     NYKRAKSRGINNIYLIDEKRAREIEPNYKGEIKKAMYSENIAVTSPYDLAIGFAEIAY
                     DNGVSFRLEEIVLDIQSIAKGFKVTTNKNKFTCRFVINTTPGEHYTIDQDKDYEQKNA
                     LKTYYLLLNDEIKEKTSNIVVKLKNEEEFIIQKNTVNGDTLIGINSNNSLGFMKSLKI
                     AKEMINSISRKHVKDIFYDNHNQDRLIIDDSRKNKGYIKVMGNHYGEVTIAPAVSKII
                     CDEIIDKLNCSLNKNFVDKRREFYRFRELSNDERNELISLDKRYGKIVCLCNGISEGE
                     IVDCIRRPLGARTVEGVKRRTGATFGTCHGAYCISKIINILARELEKKPTEIVEDSKN
                     SKVLASRIKEFDRV"
     gene            complement(4141..6897)
                     /locus_tag="K8M77_002047"
     CDS             complement(4141..6897)
                     /locus_tag="K8M77_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HBI7086518.1"
                     /translation="MKNKILNTVLISILLLVVVFFVSTNFIINFQWFKEVGYLNVFFT
                     KLIAISKLFVPIFILYFCVIAIYLFTLRKSIRSLVGDTKFKSVKKYFLLSNLVISILG
                     AGATATTQWYKILQFTNAVSFGEVDPIFNKDISFYVFKLPLVQSLFSTAISLIIILVL
                     ITVIIYLALGFKDKIYQNKDNVININSKTYGIRKFAGKQLAVLASVLSLLIGCSYLLK
                     SYNLVYSTRGVSYGAGYTDVKITMIFYKVIAIACVISSIVVFISILKLKFRPIIISIA
                     SIAVLIVLEPVVAIFTQQFVVKPNEMELEKPYISYSIDATKKAFNIDEIEVKEMEPNE
                     NITSEKLEDNKDIIENLKVNSTGPLLSFYQQVQLIKNYYEFNDADTDRYNINGKYTQV
                     FVSPREINREAMTTWQNKHLRYTHGYGLAMSRVNSVTEFGQPDFVMKDIPTVNTTDIN
                     LENPRIYFGESDNDYVIVNTEGGEFDYPTGDTENTFNYNGTGGLKMTPFNRVLFSIYE
                     RNPKILMSSSITSESRIILNRNIVKRVQEIAPFLTYDSDPYIVVHDGRLVWMMNAYTS
                     TDKYPFSEPHEGVNYIRNSVKVVVDAFNGNVDFYVTDENDPIINCYLKIYKGLFKPLS
                     EMPEDLKEHFRYPQDLFELQSKVLTKYHVDDPIKLFTEEDLWDRSLDVVKHGGDNLSQ
                     GDEGKEESILNKAKENKNNEAENEGLYLMTKLPDEENVEMMLLDYFNMRGKQSMVALL
                     GARMDGDNYGELVMYKFPPQRTIYSPILFKNRIQQDPNISKEISLWAGKGSEVIYGDI
                     IIVPIEDSLLYLNTIYLKANSENSMPEMKRVILSNGDKIVIEENIEKALLKLFNYNSF
                     EENKNSNKDETSNTEITSDNSGVKEAADLFNKAIEAQKNGDWATYGEFINKLGDVLNK
                     MSHD"
BASE COUNT         2308 a         1285 c          680 g         2788 t
ORIGIN      
        1 taaaatttaa aacttattta ttatctatcc ttatagcaat atttacaacc acatcccggt
       61 cttcctggct tttctggttt ttctggtttt tcaggtcttt caggtctttc attatttttg
      121 cactttttat aacattcaac tgtagcaaat aaatgtttga aagcttcaaa aattgtctta
      181 tctaattctt catctaattc cattgcttca cataataaag atttttgttt ttttcttaaa
      241 cattctaatc tttcttctaa ttcatcaatt tctctttgaa tatattttac accttcaagt
      301 ttttcttcaa ctgcttcatt aagctcagca gattctctag ttaactcttc agctattttc
      361 tctaactttc tgcacttatt gcagttatcc tcatatttat ccaagttaca ttcacaatta
      421 tatttatagt tatatttaca attatccata ataattacac ctcactttta tttcacctaa
      481 tttatactat gttaataata tgttttttga cacaaaaaat aaaaaaaacg atattaaaaa
      541 taccgttttt ttaactctat actaaactta tttattttaa tatataacta taaatctaat
      601 taaatatttc tcctataaag aaattatatt actttaataa atcacatctc atatattctt
      661 ttaaagcttc tggtataact actgatccat cttcttgttg atagttttct aatatagcag
      721 ctacagttct tccaatagca acccctgatc catttaatgt atgaacaaat tgtggttttt
      781 cctttggaga atttttatat ttaatgttag ctcttctagc ttggaaatct tcaaagtttg
      841 aacaacttga aatttcaaca tatctattgt agcttggcat ccaaacttct atatcatatt
      901 ttaatgcagc agtgaatcct aaatcacctt tacatattct aactattcta tatggtaatc
      961 ctaaaccttg taatacagat tcagcatctt gaactaattt atctaattct tcatagcttt
     1021 gctctggttt acagaactta actaactcaa ctttattaaa ttggtgttgt cttataagcc
     1081 ctcttgtatc tcttcctgct gaaccagctt ctgctctgaa acaagcacta tatgcagcat
     1141 gttttattgg aagtttatct ccctctaaag tttcatttct gtacatatta gttacaggaa
     1201 cctcagctgt tggtattaag aaatatccat tgttttctac tttgaaagca tcttcttcga
     1261 attttggaag ttgaccagta cctgtcatac tatctctatt taccatgtat ggtggtaata
     1321 cttcagtgta tccattttca aatgtgtgtt tatctaagaa gtagtttatt atagatctct
     1381 ctaatctagc acccattcct ttgtaaactg taaatcttga tccagcaacc tttccacctc
     1441 tttcgaagtc taaaagatct aaatctgttc ctaaatccca gtgtgctttt ggttcaaagt
     1501 tgaatttagt aggctctccc catcttttta tttcaacatt gtcctcatca gtttcccctt
     1561 ctggaacagc tgggtttggt atattaggaa ttcttagcat gatgtagtta attttatcgt
     1621 ttaactcagc aactttagca tcatcagcct ttattttatc tcctatttct ctcatttcag
     1681 ccattacttc tgtaacgtct tctccagctt tttttctctt tggaatttca gctgatactt
     1741 catttctttg tttctttaaa gcctcaacat caactaaaat ttttcttctt tcttcatcta
     1801 aagctataac ttcgtctata acatttacat caaagtcttc tcctctatta gataaagctt
     1861 ttttcacttc ttcagtatta cttcttaact tttttaaatc taacattgcc attctccttt
     1921 caagaacctt atttatattt aaaattttat taactaaaaa aatatataaa aaaggagcct
     1981 ccattcccta caaaaaggga cgaaaagact ccgcgttgcc accctaattg ataaaatcaa
     2041 aaaaagattt tatcctctta aaaactttaa cggttaattc cgtactgctc atcacagtcc
     2101 ctcacaggtg gattcatgaa aatattgtac tagttcacac caaaaccact agctctctga
     2161 aagcaatata taacattact agcctgatca acggtttatt attaatttaa acataatata
     2221 taaaaaatat actttaatat ttgctttttg tcaattctta actatctttt ggtagatttt
     2281 ttgtgcatat aatgtctacg ccagtattca ttatattttt acataccaca tttccacttt
     2341 tagttggcac tccaacgtat aatctactta aaacatttga aaaatcaatc cacttagata
     2401 tctcaactgg tttattactt tttacaggaa ctacattata ttttttagat cctctaactc
     2461 ttaccacact agtaaatata tctttatgca ttaaaccacc cctttagact ctatcaaatt
     2521 ccttaattct agatgctaaa accttagaat tctttgaatc ctcaactatt tctgttggct
     2581 ttttttcaag ctctcttgct aatatattta ttattttact tatacagtaa gctccatgac
     2641 aagttccaaa ggtagcacct gttcttcttt ttacaccctc aacagttctt gcccctagtg
     2701 gacgtcttat gcagtctaca atttcacctt cactaatacc attacaaagg catactatct
     2761 ttccatacct cttatctaaa gatataagtt catttctctc atcattagat aattctctaa
     2821 aacgatagaa ttcccttctt ttatctacaa agtttttatt taaactgcaa tttaatttat
     2881 ctattatctc atcacatatt atcttagata cagctggggc tatagtaacc tctccatagt
     2941 gattacccat aacctttata tatcctttat tttttctact atcatctata atcaatctat
     3001 cctgattatg attatcatag aaaatatcct taacatgttt tctactaatg ctatttatca
     3061 tttctttagc tatttttaat gacttcataa atcctaatga attatttgag tttataccta
     3121 ttaaagtatc cccattaaca gtattctttt gaattataaa ctcctcttca ttttttaatt
     3181 taacaactat atttgatgtt ttctctttta tttcatcatt taataatagg taatatgttt
     3241 ttaaagcatt tttttgttca taatccttat cttgatctat agtataatgt tcacctggag
     3301 ttgtatttat aacaaatcta caagtaaatt tattcttatt cgtagttact ttaaagcctt
     3361 tagctatgct ttgaatatct aaaactattt cctctaatct aaagcttact ccattatcat
     3421 aagctatttc agcaaatcct atagctaaat catatggact tgtaacagct atattttctg
     3481 aatacatagc tttctttatt tcacctttat aattaggttc tatttctctt gctctttttt
     3541 catcaattaa ataaatatta tttattcctc ttgatttagc tctcttatag ttctcttcaa
     3601 ttattttaac tgtttcatca ttatcagcta tagttaaatg tcctaccctc ttaaatggaa
     3661 catttaattt tttagttatt tcaggtaaca tcatgtttcc catattctct aagtaactca
     3721 ttaaattgtc cttgcattca gaaccgtcat atacaattgc tgtattaaca aaggaaacat
     3781 catctgcaat atcaaaatcc ttctctataa gagctatatt taagttatac tttgataact
     3841 catatgcaac agagcatcct atgattcctc cacctaatat caacacatca taatccatat
     3901 gtttcacccc tagctcttag atatattcca ctaaaaatta tatcataaaa tatatatact
     3961 attgtaacca ttcctaaaat tatagcaata taaaaaaaag agtattcaag taaatttcta
     4021 taaaatctta ataaatcaaa attaaaaaat ctacttaaaa tactcaaatt tattttatat
     4081 aaattacaat ataaaaattt taactaattc ttagttattt tttaagaacc aataattaat
     4141 ttaatcatgc gacattttgt ttaaaacatc acctaattta tttataaact ctccataagt
     4201 tgcccagtca ccattttttt gagcctctat agctttatta aataaatcag cagcttcctt
     4261 tactccactg ttatctgaag ttatttcagt atttgaagtt tcatccttat ttgagttttt
     4321 attctcttca aatgaattat aattaaatag ctttaaaagt gccttttcta tattttcttc
     4381 tataactatt ttatctccat tagaaagaat tactcttttc atttcaggca tactattttc
     4441 tgaatttgcc tttagataaa ttgtatttaa atatagtaat gaatcttcta taggaacaat
     4501 aattatatcc ccatatataa cctcagatcc tttcccagcc caaagactta tttccttaga
     4561 aatgttagga tcctgttgta ttctattctt aaataaaatt ggactatata tagttctttg
     4621 aggagggaat ttatacataa ctagttctcc atagttatct ccatccattc tagctcctaa
     4681 aagtgctacc atactttgtt tgcctctcat attaaaataa tctagaagca tcatttcaac
     4741 attttcttca tctggtaatt tcgtcataag ataaaggcct tcattttcag cttcattgtt
     4801 tttattttct tttgccttat taagaataga ttcttcctta ccttcatctc cttgacttag
     4861 gttatctcct ccatgcttaa caacatctag tgatctatcc cataaatctt cctcagtaaa
     4921 tagtttaata ggatcatcta catgatattt tgttaatacc ttactttgta attcaaataa
     4981 gtcttgagga tatctaaaat gctcctttaa atcctctggc atttctgata aaggcttaaa
     5041 taaaccttta tatattttta aataacaatt aattataggg tcattctcat ctgttacata
     5101 aaaatctaca tttccattaa aagcatcaac cacaacctta actgaattcc ttatatagtt
     5161 tactccttca tgaggctctg aaaatgggta cttatctgtt gatgtatatg cattcatcat
     5221 ccaaaccaat cttccatcat gaactactat atatggatca ctatcataag ttaaaaacgg
     5281 agctatttct tgaactctct taactatatt acgattaaga attattctac tttcagaagt
     5341 tatggaacta gacattaata ttttaggatt tctttcataa atactaaata acactctatt
     5401 aaaaggtgtc atctttaatc caccagttcc attatagtta aatgtatttt cagtatctcc
     5461 tgttggatag tcaaactctc ccccttcagt atttacaata acataatcat tatcactttc
     5521 tccaaaataa attcttggat tttcaagatt aatatctgtt gtatttactg ttggtatatc
     5581 tttcataaca aaatcaggtt gtccaaattc tgttacacta ttaaccctac tcatagctaa
     5641 tccataccca tgggtatatc taagatgctt attttgccat gttgtcatag cttctctatt
     5701 tatttccctt ggagatacaa atacttgagt atatttacca tttatattat atctatctgt
     5761 atctgcatca ttaaattcat aataattttt tataagttga acttgctgat aaaaacttaa
     5821 taaaggtcct gttgaattaa cctttaggtt ttcaataata tctttattat cttctaattt
     5881 ttcactagtt atattttcgt ttggttccat ttctttaact tcaatctcat caatattaaa
     5941 agccttttta gtagcatcaa tactatatga aatataaggt ttttcaagtt ccatttcatt
     6001 tggtttaact acaaactgtt gagtaaaaat ggctactaca ggctctaaaa caataagtac
     6061 agcaattgat gcaattgata ttataatagg tctaaattta agctttaaaa ttgaaataaa
     6121 aactactatt gaagatatta cacaagctat agctataacc ttataaaata tcattgttat
     6181 tttaacatct gtataaccag ctccatagga tacccctcta gtactataaa ctaaattata
     6241 tgactttaat aaatagctac atcctataag cagtgataat actgaagcta aaactgctag
     6301 ttgttttcct gcaaactttc ttattccata agttttacta tttatattta taacgttatc
     6361 cttgttttga taaattttat ctttaaaccc taaagctaaa tatataataa ctgttataag
     6421 aactaaaata attattaggg aaatagctgt actaaaaaga gactgtacta aaggtaattt
     6481 aaaaacataa aaagatatat ccttattaaa aataggatct acttctccaa aagaaaccgc
     6541 attagtaaat tgaagaattt tataccactg agttgtggca gtagctccag ctcctaatat
     6601 tgatattact aaatttgata ataaaaagta cttcttaact gacttaaact tagtatcacc
     6661 taccaagctt ctaatacttt ttctcaaagt aaataaatat atagctatta cacaaaaata
     6721 taaaataaat ataggtacaa ataatttact tatagctata agcttagtaa aaaatacatt
     6781 taaatatcct acttccttaa accattgaaa attaataata aaattagttg aaacgaaaaa
     6841 aactacaact agaagtaaga tactaataag aactgtatta agtatcttat ttttcaaaac
     6901 ttcccctcct taaattttga catttcatat atataaaata aaacttcact aaaactaaaa
     6961 ttcatataag ccttaaatct taatcaatat taaataaact ttactctttg caaatgaaag
     7021 tctaaagaat atattagata aatattaatc taaatattta a
//
LOCUS       DAEVGI010000129         7007 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEVGI010000129 DAEVGI010000000
VERSION     DAEVGI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.129"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            60..134
                     /locus_tag="K8M77_002048"
     tRNA            60..134
                     /locus_tag="K8M77_002048"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Cys,seq:gca)
     gene            complement(505..1386)
                     /locus_tag="K8M77_002049"
     CDS             complement(505..1386)
                     /locus_tag="K8M77_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBI7086519.1"
                     /translation="MVKFQKLKIGDTIGIVSPASGDSTDVINYNISSFKNLGFKIKEG
                     KYLRKKNDYLAASDKERAEDLMEMFKDKEVKAIVAYRGGYGCIRMLPYLDIGVIKKNP
                     KILCGYSDLTVLLNYASQKTGLITFHGPMINSKISSDEITKNSFLSLLTDESNLINIS
                     TKDFKVENKELFRGILCGGNLTMICSTLGTPYEINTRNKILMIEDVNEENYAIDRYLM
                     QLKLSGKLDSCRAFLIGHFTPYNPKTINTILSILLPYKKPIIYNIPFGHDYPNITLPI
                     GSSILFDPAKDKLIIKY"
     gene            complement(1478..2422)
                     /locus_tag="K8M77_002050"
     CDS             complement(1478..2422)
                     /locus_tag="K8M77_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBI7086520.1"
                     /translation="MIQIYKSLSENDGTLKKITSLEPGCWVNIIAPSQEELLLISKKT
                     GVPLEFLRAPLDDEETSRIEIEGDFILIIVDIPFTEMEDNSLTYDTYPLAIIHTKNEL
                     ITVCLKNSKVLTDFSSNKVRNFYSFKKSRFILQILNRVSTYYLLYLRQIDKKSIMIEK
                     RLHKSMKNRELVQLHSLSKSLVYFSTSLKSNEITLEKMLKLDVIQRYEEDKDVLEDVI
                     IENKQAIEMTDIYSNILGSTMDFFASVISNNLNIVMKVLASVTILMSTLTVISGIYGM
                     NFDYLPLLHHPYGFHIIMTLSVILCGLIALILYKKDMF"
     gene            2697..3194
                     /locus_tag="K8M77_002051"
     CDS             2697..3194
                     /locus_tag="K8M77_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086521.1"
                     /translation="MGTMHKEVVKKRRDVAIVISIICMSVAISLSNFFGNIKFESFNA
                     ETITDPIFLVLTLVIFFREYRKCKTSYKYSIVANQLMIHKVKANKQSTLENIKLNNIV
                     YLGKDYSKEAKDFKTSSCKKYVCSLLDRTGHYCCIYKNGDGYNKFYFKPSKTLVDKLK
                     KNLAI"
     gene            complement(3223..4392)
                     /locus_tag="K8M77_002052"
     CDS             complement(3223..4392)
                     /locus_tag="K8M77_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HBI7086522.1"
                     /translation="MRQEVISYLSTEKEDLFNLCKFLHDNPEDSYKEYDACKYICNFL
                     RDRDFDVREKFLDLDTAFYAKKGNGYPKICFLCEYDAIKSKGGHITGHNLLTTISITS
                     ALGLSKVIEKSGGTVILIGCPGEYLGGTKSTYVRQGIFDDIDVVLTAHPDIVTSESGT
                     SKAIIPLKVTFKGHDGLSFLDFDSFTSLDGTMLTFNVINALSKGFCKKANIHTILSQG
                     GVTPLLRPVESEGKLYIRAESTKTAKCIENKIKNLVACISDTMNLESCVELYEPPNEE
                     LLSNQVLNRLYSHNLKENGIINIGKPRDIDAGISLGTVSHKVPCIHPYINIVDNVNID
                     YGTKEFADATISSFAQEQALSASYALISTALDLIESNFLLSEVKETFNMSKENLY"
     gene            4548..4724
                     /locus_tag="K8M77_002053"
     CDS             4548..4724
                     /locus_tag="K8M77_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor G inhibitor Gin"
                     /protein_id="HBI7086523.1"
                     /translation="MNKSICIICGKEGHGIMIRGKLICTECEKKAISCDINSEFYEFY
                     KNRLKEEVYKKKLG"
     gene            4742..6172
                     /locus_tag="K8M77_002054"
     CDS             4742..6172
                     /locus_tag="K8M77_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBI7086524.1"
                     /translation="MRNIPIINGISMYLREEIVPFSMPGHKNGRGFKGFEEILLQGDL
                     TEVEGLDNLQHPEGIIKEAEDKLSKLYKSKKSYFLVNGSTCGNLIMIFSLFKEGDKVL
                     VERNFHKSIMNGIIMRKLKPVFINNIFHSKLKAPVGIDLNHLKEQISLNKDLKGIILT
                     YPNYYGVGVNIDETIKIAREANLKVGIDSAHGAHFGFNKLLPESVQELKTDIVVMSAH
                     KTLPSLTQTAWMHVNNEDFIEEIEFYKNIFMSTSPSYMFMMSLDYSRNFLEEHGEEAY
                     NNLFKIIEDFKNHIKDLSYFKILDKEFLINGLEKNIKINDIKIDNSRIVLNLRENLNG
                     NKLLDYLRSKNIQCEMSDNKNIVLIPSPFNTEEDFKILKDALKYCDINCLKDEEIDFY
                     LTDLPEKKLEPFEVLELEYERININDAVGKIVAESIVLYPPGVPMLIMGEIIEERHIK
                     LLNEYMKSKVDLIGIKDKKVKVIKNK"
     gene            6419..>7007
                     /locus_tag="K8M77_002055"
     CDS             6419..>7007
                     /locus_tag="K8M77_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBI7086525.1"
                     /translation="MGKIFCIMGKSGSGKDTILKEINKVEDLNLKTIVTYTTRPKRNN
                     ETDGVEYFFIDKEKLDDFNREGKIIECRVYDTVHGKWYYLTVDDGQINLEENNYIVIV
                     TLEAYSEFRKYFGDEKLVPLYIDLDDGVRLERALKREREQKSPNYNEMCRRFLADNED
                     FSEDKLLNNNIIKKYNNYNLEKCISEICDVIRKTSK"
BASE COUNT         2636 a          807 c         1061 g         2503 t
ORIGIN      
        1 gagcagagga ctgaaaatcc tcgtgtcgct ggttcgattc ctgctcgagc caccagtgtg
       61 gcgctatagc caagtggtaa ggcagaggtc tgcaaaacct ttattccccg gttcaaatcc
      121 gggtggcgcc tccataaaaa acctcagtaa agttactgag gtttttgctt tgtagaaatt
      181 tacatataca cactaaattt tattccaaac actaagaatt aagtggattt atattttata
      241 aattcacttt tttcattaaa tattcaaaaa gacatgttaa tatgtctttt ttagtttatt
      301 tagcataaat aaaagtatat gttatgcagt agttagaact ctctccttta ttcttcttaa
      361 actttattat aaataaaagt acattttatt tgctagctaa taaattttta tagctttagt
      421 gcaatctacg tgttttattg atggttatga tattagttta cccttagcta gaatattgac
      481 tttcctatta accataagag acatttagta ttttattatt aatttatctt tagctgggtc
      541 gaaaagaatt gatgatccta ttggtaaggt tatattagga tagtcatgac caaaaggaat
      601 attatatata attggttttt tatatggcaa tagtatagaa agtatagtat taattgtttt
      661 tggattatat ggggtgaaat gacctattaa aaaagctcta caagaatcta gtttacctga
      721 aagttttaat tgcattaagt atctatctat agcataattc tcttcattta catcttcaat
      781 catgagaatt ttatttctag tatttatttc ataaggagtt cctaaggtgc tacatatcat
      841 agttagattt ccaccacaaa gaatccctct aaatagttct ttattttcaa ctttaaaatc
      901 ctttgtagat atattaatta aattgctctc atcagtaaga agtgatagaa agctattttt
      961 agttatttca tctgaactaa ttttagaatt tatcataggt ccatgaaatg ttattaagcc
     1021 tgtcttttga gatgcgtaat ttaagagaac tgttaaatca ctgtaaccac ataaaatttt
     1081 tgggtttttt ttaattactc ctatatctaa atagggtaac attcttatac atccataccc
     1141 acctctataa gctactattg ctttaacttc cttatcctta aacatctcca ttaaatcttc
     1201 agctctttct ttgtcagaag cagctagata atcatttttt tttcttaaat attttccttc
     1261 tttaatttta aagccaagat ttttaaatga agatatatta tagtttatta catcggtact
     1321 atctccacta gcaggggaaa caatacctat agtatcacca atttttaatt tttgaaattt
     1381 caccataaaa cctcctaaaa aaatagagag cataaattgc tctcaataat cttttagtta
     1441 attatatgca aaaatctttt tatggttaca gataaattta aaacatatct tttttatata
     1501 gtataagtgc tattaaacca caaagtatta cagataaagt cattattata tggaacccat
     1561 atggatgatg taatagtggt aaatagtcaa agttcattcc gtatatacca gatataactg
     1621 taagggtaga cataaggatt gtaactgaag ctaagacttt catcactatg tttaagttat
     1681 tagaaataac agaagcaaag aaatccatgg tactacctag aatgttacta taaatatcag
     1741 tcatctcaat agcctgctta ttttctatta ttacatcttc taaaacatct ttatcttctt
     1801 catatctttg aataacatca agttttaaca tcttttctaa tgtgatttca ttagatttta
     1861 aggatgttga gaaatatact aatgatttac ttaatgagtg caattgtact aattctctgt
     1921 ttttcattga tttatgaaga cgcttttcta tcataatact ttttttatct atttgtctta
     1981 agtataaaag ataatatgta gaaactctgt ttagaatttg taaaataaat cttgattttt
     2041 taaatgagta aaaattacga actttatttg aagagaagtc ggttaatacc ttgctatttt
     2101 ttaagcatac agtaattaat tcattttttg tatgaattat tgctaatgga taagtatcat
     2161 atgttaagga gttatcctcc atttctgtaa aaggaatatc gactattatt aatataaaat
     2221 ctccttcaat ttctatacga gaagtttctt catcatctaa aggcgctctt aaaaattcta
     2281 aaggaactcc tgttttttta gaaataagaa gtaattcttc ctgagaaggt gctattatat
     2341 taacccaaca acctggttca agggaagtaa ttttctttaa agtcccatca ttttcactta
     2401 aagatttata tatttgaatc attttctccc tcccaaaaca ttaagttata ttttgtttca
     2461 attatcatta aacaagttat ttaaaataaa atcaagataa tttttataat tagcttagat
     2521 ataataaaga aagcaataga aataaagagg tacaagcaat acaattacta taaactttaa
     2581 atatgagcaa ttaaggttaa aatcggtaaa attttatact tatctaggta gatttaattt
     2641 ttgaaaatta gtataatgtg atttgtttat aatattaaaa gtcgggggat ttttaaatgg
     2701 gcactatgca taaggaagtt gtcaaaaaaa gaagggatgt agcaattgta attagtataa
     2761 tctgtatgag tgtagctata agcctatcta atttttttgg caatattaaa tttgagtctt
     2821 ttaatgcaga gacaataaca gatccaatat ttctagtttt aacattagtt atattcttta
     2881 gagaatatag aaaatgtaag acaagttata aatattccat agtagctaat caactaatga
     2941 ttcataaggt gaaagctaat aaacaatcaa ctttagagaa cataaagtta aataacattg
     3001 tttatttagg taaagattat tctaaagaag caaaggattt taaaacctct tcttgtaaga
     3061 aatatgtgtg tagtttatta gatagaacag gtcattattg ttgtatatat aaaaatggtg
     3121 atggatataa taaattctat tttaagccaa gtaaaacact tgttgataaa ttaaaaaaaa
     3181 acttagctat ttaagaaaag agagtcttaa gactctcttt ttttaatata aattttcctt
     3241 agacatattg aaggtttctt taacttctga aagcagaaaa ttgctttcta taagatctaa
     3301 ggctgttgaa atgagggcat aagaagctga aagagcttgt tcttgagcaa aggatgatat
     3361 tgttgcatct gcaaattctt tagttccata atcaatattt acattatcaa ctatgtttat
     3421 ataaggatga atacaaggta ccttgtgact tacagttcct aatgaaatac cagcatctat
     3481 atctcgtggt tttcctatgt ttattattcc gttttctttt aaattatgac tatataatct
     3541 atttaatact tgattagata aaagctcttc attaggtggt tcataaagct caacacaact
     3601 ttctaagttc attgtgtcag aaatacaagc aacaaggttt tttattttat tttctataca
     3661 ttttgcagtt ttggtacttt ctgctcttat atataattta ccttctgatt caacaggtct
     3721 tagaagaggt gtaacgcctc cttgagataa aatagtgtgt atgttagctt tcttacagaa
     3781 gcccttagat aaagcgttta ttacattaaa agttagcata gtaccatcta aagatgtaaa
     3841 actatcaaaa tctaagaaac ttaatccatc atgaccttta aaagttactt ttagaggtat
     3901 aattgccttt gaagtaccac tttcagaggt tactatatct gggtgagctg tcaatactac
     3961 atctatatca tcaaaaatac cttgtctaac ataagtagat ttagttccac ctaaatattc
     4021 tccaggacat cctattaata ttactgttcc accagacttt tcaattacct tgcttaatcc
     4081 taaggcactt gtaattgata tagtagtaag aagattatga cctgttatat gtcctccctt
     4141 agatttaata gcatcatatt cgcataaaaa gcatatttta gggtatccat ttcctttctt
     4201 agcatagaaa gcagtatcta aatctaaaaa tttttctctt acatcaaaat ctctatctct
     4261 taggaaatta catatatatt tgcaagcgtc atattctttg taactatctt caggattatc
     4321 atgcaaaaat ttacaaagat taaagagatc ttctttttct gtagataaat aagaaataac
     4381 ttcctgtctc attgaaaaaa cctcctaaat atttataaga ttagtttgtg ctacaataga
     4441 aaaaatatta atttttattt atttgaatat ttttcctgct gaattatgta tagaaaaata
     4501 ttatgtggaa aaaatataga aaaatagaga tttaaggagt gttatctatg aataagtcaa
     4561 tttgtataat ttgtggcaaa gagggtcatg gtataatgat aagaggaaaa ttaatatgca
     4621 cagaatgtga gaaaaaagct ataagttgtg atattaatag tgaattctat gagttttata
     4681 aaaatagatt aaaagaagaa gtttataaga aaaaattagg ataacagagg agtggttaac
     4741 tttgagaaat attcctataa ttaatggaat atcaatgtac ttaagagaag aaatagtacc
     4801 tttttctatg ccaggtcata agaatggacg aggctttaag ggatttgaag aaatactttt
     4861 acaaggtgat ttaactgaag ttgaaggatt agataacttg cagcatccag agggaataat
     4921 caaagaagct gaggataaac tttctaagtt atataaaagt aaaaagagtt attttttagt
     4981 taatggaagt acttgtggaa atttaataat gatattttct ttgtttaaag aaggagataa
     5041 agttttagta gaaagaaact tccacaaaag tataatgaat ggaataataa tgagaaaatt
     5101 aaaaccagtt tttataaata atatatttca ttcaaaacta aaagcaccag taggaattga
     5161 tctaaatcat ttaaaagagc aaataagttt aaataaggat ttaaaaggaa taatattaac
     5221 ttaccctaat tattatggcg taggagttaa tatagatgag actataaaaa tagctagaga
     5281 ggcaaattta aaagtgggaa tagattcagc tcatggagca cattttggat ttaacaagtt
     5341 attgcctgaa agtgtacaag aattaaaaac tgatatagtt gtaatgagtg ctcataaaac
     5401 tttaccaagt ttaacacaga ctgcttggat gcatgttaac aatgaagatt ttattgagga
     5461 aatagaattt tataaaaata tttttatgag tactagccct tcatatatgt ttatgatgag
     5521 tttagattat tcaagaaact ttttagaaga acatggtgaa gaggcatata ataacttatt
     5581 taagatcata gaggatttta aaaatcatat taaggactta tcatatttta aaatattaga
     5641 taaggaattt ttaattaatg ggttagaaaa aaatattaag ataaatgata ttaagataga
     5701 taatagtaga atagtactaa acttaaggga aaacttaaat ggtaataaac tattagatta
     5761 tttaagaagt aaaaatatac agtgtgaaat gagtgataat aaaaatattg ttttaatacc
     5821 gtcaccattt aatactgaag aagattttaa aattttaaaa gatgctttaa aatattgtga
     5881 tataaattgt cttaaagatg aagaaataga tttttattta acagatttac cagagaaaaa
     5941 attagaacct tttgaagttt tggagttaga gtatgaaaga attaatataa atgatgctgt
     6001 aggtaaaata gtggcagaga gtatagttct gtatccacca ggagtcccta tgttaataat
     6061 gggtgaaata atagaagaaa gacatattaa attacttaat gagtatatga aaagcaaggt
     6121 agatttaata ggtataaagg ataaaaaagt taaggtaata aagaataagt aaatacttaa
     6181 agaatatatt agttaggttg tatcgaagtt aggagttaaa taaggaatat aataagtatt
     6241 ttttatttat aagattttgg tacaaccttt ttaatttatt aaataaacgg attagaagat
     6301 tatttgatat aataatcctt gtattaaaat tattacataa aactttttag ggagttttat
     6361 agaatttatt taatatattt ttagagaaaa aaacgtgttt atatagattg gagtagttat
     6421 gggtaaaata ttttgcataa tggggaaaag tggttctggt aaggatacta ttttaaaaga
     6481 gataaataag gttgaagatt taaatttaaa aactatagtt acatatacaa ctagacctaa
     6541 acgaaataat gaaacagatg gagtggaata tttctttata gataaggaaa agctagatga
     6601 ttttaataga gaaggtaaga taatagaatg tagagtttat gatactgttc atggaaaatg
     6661 gtattaccta actgtagatg atggccaaat aaacttagaa gaaaataatt atatagttat
     6721 tgtaacttta gaggcttatt ctgagtttag aaaatatttt ggagatgaga aattagtacc
     6781 actttacata gacttagatg atggagttag acttgaaaga gcattaaaaa gagagagaga
     6841 acagaaaagt cctaattata atgagatgtg cagaagattt ttagcagata atgaagattt
     6901 tagtgaagat aaattactaa ataacaatat aattaagaaa tataacaatt acaatttaga
     6961 aaaatgtata agtgaaatat gtgatgtaat aagaaaaact tctaaat
//
LOCUS       DAEVGI010000130         6973 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEVGI010000130 DAEVGI010000000
VERSION     DAEVGI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.130"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            165..752
                     /locus_tag="K8M77_002056"
     CDS             165..752
                     /locus_tag="K8M77_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBI7086526.1"
                     /translation="MKSLKGTKTAENLMKSFAGECQARTRYTYFSSTARKEGYVQISN
                     IFLETAENEKEHAKRFYKFLKDDLQGEAVEINAAYPVELPTDTLTNLKFAADGEHDEL
                     SNLYPSFADVADEEGFPEVAAAFRMIAKAETAHYNRFMKLAKNMEEGKVFKKDEVVLW
                     KCGNCGFIWEGAEAPLKCPSCLHPQAFFEVFKETY"
     gene            complement(914..1459)
                     /locus_tag="K8M77_002057"
     CDS             complement(914..1459)
                     /locus_tag="K8M77_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HBI7086527.1"
                     /translation="MLYYLVALFDKVSYAIIKPLQESISNEYKLYENLPMLHITLAVI
                     EDPDMDKLNEVVKNTLKSYKKFEVHLNGLIKFGEPYRSVNLKVDEESGVIKKLSRELY
                     ENLSKEGFKLTSNPDTWQLHVSLANPYFAQRVWGEDEFESAYTKLQDYNFDRELLIDE
                     IQFWRPINDENKMVVFSYPLH"
     gene            2282..3838
                     /locus_tag="K8M77_002058"
     CDS             2282..3838
                     /locus_tag="K8M77_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBI7086528.1"
                     /translation="MSILIVKNMNHGFGDRAIFEDVSFRLLKGEHVGLIGANGEGKST
                     FMNIITGKLMPDEGDVQWSNKVRVGYMDQHAQLEKGKTIREVLREAFQYLFDMEVEMN
                     GLYERMGEMSEDEMTKALERTATIQDMLDHNGFYMIDAKVEEVAAGLGLRDVGLDKDV
                     TDLSGGQRTKVLLAKLLLETPDILLLDEPTNYLDEEHIAWLKNYLIGYENAFILISHD
                     IPFLNSVVNVIYHVENRALTRYTGDYDNFMRVYEANKKQLEAAYERQQAEIAKLEDFV
                     ARNKARVATTGMARARQKKLDKMDKIELAAEKPKPEFNFKQGRTSGKLIFEARDLVIG
                     YDEPLSKPLNIRMERGQKIALVGANGLGKSTLLKSLMGLIKPISGEVETGDYQSIGYF
                     EQEIKEANYNSCIEEVWQEFPGYTQYEVRSALAKCGLTTKHIESSVAVLSGGEQAKVR
                     LCKLINKETNILILDEPTNHLDVEAKDELKRALKEYKGSILLVCHEPEFYRDVVTDVW
                     SCEEWTTKIV"
     gene            4193..6193
                     /locus_tag="K8M77_002059"
     CDS             4193..6193
                     /locus_tag="K8M77_002059"
                     /inference="COORDINATES: protein motif:HMM:NF024573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7086529.1"
                     /translation="MKLSKLRIKNYKSFKDSGTIEIKGNIFALIGQNNAGKSAIMDAI
                     QVFFPNCKKSANSENFHKFTKEDISIELWFSKVNSDYLENKLFQDKIKKQNEVIKKCI
                     ETEKYDKELKKLDEIREKELNKCILKYGIENEELYVKKIIPKESKAFYILKNNDKISE
                     ADLKKILPELKIIPAIRDPKNESTAGTNSYLKDLIQMLDEEAQTEIMMEDKKLTYKEL
                     NEVLSEETSKRSKELSSKITNYYSKAIGSSDFEVKVNSSVNISKGTNYSTELIDNTTN
                     IKADILDCGTGYQSMVILALLETYVEIAHTNNNYILIIEEPEVYLHPRLQRKMIDTLL
                     RVSEHNQVIFTTHSPITISKLNSLDVKLVEREYGEAKIKNSSVKEIIDELGVRADDFL
                     HSKGIIFVEGKDDEIVIRELLKKLDKCIIDKINIIQAVGCSNFKWYANAEILINKNFD
                     IPTLILRDSDVERPEKLKKDLVEDIINSFINTPQYARYNQDEKEEIKTKINDAIKVLS
                     QHSLEYYFINPNLLVQFYGCKVELENAIKCYNCKYNDKMQEGLSGKDIEGKVQNYYQP
                     KRFLEGYPDKPSDIAKNLEPKFIEEWESYKESCNCEISELYGIDNFIKIRNVLKNNIN
                     DFNKSNDVFKEIINMYDIKYLEENGLKELIDILKEFIIKVKI"
     gene            complement(6276..6437)
                     /locus_tag="K8M77_002060"
     CDS             complement(6276..6437)
                     /locus_tag="K8M77_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBI7086530.1"
                     /translation="MPRKDLKNRTPVGSAMDNKIYYEMKAYSEKTGIPISKILDKAMS
                     MYLESTKKL"
     gene            6548..>6973
                     /locus_tag="K8M77_002061"
     CDS             6548..>6973
                     /locus_tag="K8M77_002061"
                     /inference="COORDINATES: protein motif:HMM:NF014548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HBI7086531.1"
                     /translation="MNDLFIKKFNNEEIITLILDNRICWIAKDVAKILNYDDPSKAIN
                     QCIKAEKFEYGIEYEVLAGDKLKEVKRLIGVTHISYLKQTPKLVIFYEEGLYGFINYS
                     KLPIGISFRKWLRREVLPELRAKGTYSINKESYKDNLKYK"
BASE COUNT         2837 a          677 c         1161 g         2298 t
ORIGIN      
        1 ttaacaaaat gttcaaaaaa tacttgaaat tttgtttgaa agtgatatta ttatttatat
       61 aaggaataac aattattaag taatataatt ttgaaattat ttatttggga aaataatttt
      121 gattgtgttt tttcaaataa gtgatttgta ggaggttttt tattatgaaa agtttaaagg
      181 gaactaaaac tgctgaaaat ctaatgaaat cattcgctgg tgaatgccaa gcaagaacaa
      241 gatatactta tttttcaagt acagctagaa aagaaggtta tgttcaaata tcaaatatat
      301 ttttagaaac tgctgaaaat gaaaaagaac acgctaagag attttataaa ttcttaaaag
      361 acgatcttca aggagaagct gtagaaataa atgctgctta cccagtagaa ttacctacag
      421 atacattaac taacttaaaa tttgctgctg acggagaaca tgatgaatta agtaatcttt
      481 atccatcatt tgctgatgta gctgatgaag aaggattccc agaagtagct gctgctttca
      541 gaatgatagc taaagcagaa actgctcact ataatagatt tatgaaatta gctaaaaata
      601 tggaagaagg aaaagtattt aaaaaagacg aagtagtact ttggaaatgt ggaaactgtg
      661 gatttatttg ggaaggtgca gaagctccat taaaatgccc ttcatgctta catccacaag
      721 ctttctttga agttttcaaa gaaacttatt aaaaatagat ttattcatgc ttaactcgta
      781 taattttatc aattgaatgt aaatataata aactaaaact tatttcagag agaagaaatt
      841 ctctctgatt tttttataaa tatagagcgt atatcaaaat aacttttgat atacgctcta
      901 taaattttaa gtgttaatgc agtggatagc taaaaaccac cattttgttt tcatcattta
      961 ttggtctcca aaattgaatt tcatcaatta aaagttctct atcaaaatta taatcttgaa
     1021 gttttgtata agctgactca aactcatcct ctccccatac tctctgtgca aaatatgggt
     1081 tagctaagga tacatgaagt tgccatgtat ctgggttaga agtcaattta aatccttctt
     1141 ttgataaatt ctcataaagt tctctagaaa gtttctttat tactccactt tcttcatcta
     1201 cttttaaatt tacagaccta taaggttctc caaatttaat tagtccattt aaatgaactt
     1261 caaatttttt atatgatttt aaagtgtttt ttactacttc atttaattta tccatatcag
     1321 gatcttcaat tacagctaag gtaatatgaa gcataggaag gttctcataa agtttatatt
     1381 catttgaaat ggattcttgc aatggcttta ttattgcata ggaaacctta tcaaatagtg
     1441 ccactaagta atatagcata gataaacccc tttcttacta gtttgtacaa tttataccaa
     1501 gtatttaatc tattatatca tatagaaact taggaagact acaaatagac cttattacaa
     1561 taggatatac acctttaaga aattggttga ttttacttaa atagaatatt aagaaaataa
     1621 ttgattttat ttaaaagtaa tattaagttt taagaacaaa ttttaaaaaa gtgtatatat
     1681 tagtttattg agggaattta taaggaatct aaaaagcttt tagtaaactg aaagcaaaaa
     1741 agagataaat tttatggaga gtaagaaaaa tatatttgaa aaactaaagt tagataatat
     1801 actatatata atgtatatta gagagtttta aaataagttt atttaatata tattttagtt
     1861 tgaaattttt gaaaggtgga acaaggaaat gatagaaagc agagtaagta gattaaataa
     1921 gaaaaaatca tctaagatgt cagttctata taatatagtt ggaatttgct ttttagcttt
     1981 aattttattt gttggtagtc aaatgtttct ttaagaattg attatttggt tggaatctat
     2041 ttgcatagat ataagttctg tataaagaat ttattttttg attatagtta aggaaattat
     2101 tttctttata tggataaggt gagtgtaaat aaaaattcca gcttaaaata tttagtattt
     2161 tacataatat tagttttagt ttagaaaata aatttttttg ttaagggata gtattttgag
     2221 ttcatatatt ttatgagatt atttttatag atatttttag ataaggagag atagaagtta
     2281 aatgagtatt ttaatagtta aaaatatgaa tcatggcttt ggagatagag ctatatttga
     2341 agatgtgtct ttcagacttt taaaggggga gcacgttgga cttataggag caaatggaga
     2401 aggtaaatca actttcatga acataattac aggtaaactt atgccagatg agggagatgt
     2461 tcaatggagt aataaggtta gagttggtta catggatcag catgctcagc ttgagaaagg
     2521 taaaactata agagaagttt taagagaagc attccaatat ctttttgata tggaagttga
     2581 aatgaatggt ctttatgaga gaatgggtga aatgtcagag gatgagatga ctaaagcctt
     2641 agaaagaaca gctacaattc aagatatgtt agatcataat ggattttata tgatagatgc
     2701 aaaggttgaa gaggttgcag ctggattagg tcttagagat gttggattag acaaggatgt
     2761 tactgattta agtggaggac aaagaactaa ggttttatta gctaaacttt tattagaaac
     2821 accagacata ttacttctag atgagcctac aaactatctt gatgaggaac atatagcttg
     2881 gcttaagaac tatttaatag gatatgagaa tgcatttatc ttaatatcac atgatattcc
     2941 attcttaaat agtgtagtaa atgtaatata ccatgttgaa aatagagctt taactagata
     3001 cactggggat tatgataact ttatgagagt atatgaagct aacaagaagc aattagaggc
     3061 tgcttatgaa agacaacagg ctgaaatagc taaacttgaa gacttcgttg ctagaaataa
     3121 ggctagggtt gctacaacag gtatggcaag agctagacaa aagaaattag ataaaatgga
     3181 taaaattgag cttgctgctg agaaaccaaa accagagttt aactttaagc aaggaagaac
     3241 ttcaggtaaa ttaatctttg aagcaagaga tttagttata ggatatgatg agccacttag
     3301 taaaccactt aacataagaa tggaaagagg acaaaaaata gccttagttg gagctaacgg
     3361 acttggaaaa tcaacacttc taaaaagctt aatgggatta ataaaaccaa taagtggaga
     3421 ggttgaaaca ggagactacc aaagcatagg atactttgag caagaaataa aagaagcaaa
     3481 ctataattca tgtatagaag aagtgtggca agagttccct ggatacactc aatacgaagt
     3541 aagaagtgcc ctagctaaat gtggattaac aactaagcac atagaaagct cagtagctgt
     3601 attaagtggg ggagaacaag ctaaggtaag actttgtaag ctaataaaca aagaaacaaa
     3661 catacttatc ctagacgagc caacaaacca tttagacgtt gaagctaaag atgaattaaa
     3721 gagagcatta aaagaatata aaggaagcat acttttagtt tgccatgaac cggaattcta
     3781 tagagatgta gtaacagatg tatggtcatg cgaagaatgg acgactaaga ttgtttagta
     3841 aaatttatga aaagagttgg tataaaaacc aactctttta tattttatat aatttatata
     3901 aatataaatt tgttggttta atgtattatt tataaatatt aacaattaag aaaatatata
     3961 gtatgataaa tttatacata tgttgtaaaa gttataatta aagatgaatt ttcctagaaa
     4021 tataattaat aaagtttaat attaattata ttagttaaat aagttttatg tttatgagga
     4081 aataagagta aatatttact tggtattgga ataattaaaa attatgtttg ggttttatat
     4141 aaatttaata gtctataatt aaataattta aggatttttt ggggggatat aagtgaaatt
     4201 aagtaaatta agaataaaaa attataagtc atttaaagac tctggaacta tagaaattaa
     4261 aggaaatata tttgcattaa ttggacaaaa taatgcagga aaatcagcaa taatggatgc
     4321 tatacaggtg tttttcccaa attgtaaaaa gagtgctaat tcagaaaatt ttcataagtt
     4381 tactaaagag gatatatcaa ttgaattatg gttttcgaaa gttaattctg attatttaga
     4441 aaataaatta tttcaagaca aaataaaaaa acaaaatgaa gtaataaaga aatgcataga
     4501 aacagaaaaa tatgataaag aattaaaaaa attagacgaa ataagagaaa aagaattaaa
     4561 taaatgtatt ttaaaatatg ggattgaaaa tgaggaactg tatgttaaaa aaattattcc
     4621 taaagaaagt aaagcttttt atatattaaa aaataatgat aaaattagtg aagctgattt
     4681 gaaaaaaatt ttacctgaat taaaaattat tccagcaata agggatccta aaaatgagag
     4741 tactgctggt acaaattcat atttaaagga tttaatacag atgcttgatg aagaagctca
     4801 aacagaaata atgatggaag ataaaaaact tacatataaa gaacttaatg aggttttatc
     4861 cgaagaaaca tctaaaagaa gtaaagaatt atcttctaag ataactaatt attatagcaa
     4921 ggcaatagga agtagtgatt ttgaagttaa agttaattca tctgtaaata taagtaaagg
     4981 aactaattat agtacggagc ttatagataa tacaacaaat attaaagcag atattctaga
     5041 ttgtggtact ggttaccaaa gtatggttat attggctttg ttagagactt atgttgaaat
     5101 agcacatact aataataatt atattttaat tattgaagaa ccagaggtgt atttacatcc
     5161 acgtttacag agaaaaatga tagatacatt acttagggtt tccgagcata atcaagtgat
     5221 ttttacaact cattctccaa taacaataag taagttaaat agcttagatg taaaattagt
     5281 tgaaagagaa tatggtgaag ctaaaataaa aaattctagc gtaaaagaaa taattgatga
     5341 gttaggagta agagcagatg attttttaca tagtaaagga attatatttg ttgaaggaaa
     5401 agatgacgag attgttataa gagaattatt aaagaaatta gataaatgta ttattgataa
     5461 aataaatata atacaagctg tggggtgtag taattttaag tggtatgcta atgccgaaat
     5521 attaattaat aaaaattttg atatacctac actaatatta agagattcag atgttgaaag
     5581 accagaaaaa ttaaaaaaag acttagttga agatataata aattcattta ttaatacacc
     5641 acaatatgct aggtacaatc aagatgaaaa agaagaaata aaaacaaaga taaatgatgc
     5701 aattaaagtt ttatcacaac attcattaga gtattatttt attaatccaa acttattagt
     5761 acaattttat ggatgtaagg tagagttaga aaatgctata aaatgttata attgtaaata
     5821 taatgataaa atgcaagaag gactaagtgg aaaagatatt gaaggtaaag tacaaaatta
     5881 ttatcagcca aaacgatttt tggaaggata tccagataag ccaagtgata tagctaaaaa
     5941 tttagaacct aaatttattg aggagtggga atcttataaa gaaagttgta attgtgaaat
     6001 tagcgaatta tatggaattg ataattttat aaaaataaga aatgttttaa aaaataatat
     6061 taatgatttt aataagagta atgatgtttt taaagagata ataaatatgt acgatataaa
     6121 atatctagaa gaaaatggat taaaagaatt aattgatatt cttaaagaat ttataataaa
     6181 agttaaaatt taatattaaa gaaaaatata taacagaata atttactaag tctaatttaa
     6241 aaaaaattag attataacta aatatacaga aagtactaaa gttttttagt actttctaag
     6301 tacatagaca tagctttatc taatatttta gatattggta tacctgtttt ttctgagtaa
     6361 gccttcattt cgtaataaat tttattatcc attgcacttc ctactggagt tctatttttt
     6421 aaatcctttc ttggcactaa taatcacctc aacaatatta ttaacctaaa agaatatttt
     6481 cataactgaa tacagttgat ataagtatgg aatactattt ttgaaaatat tttaggagga
     6541 atgagttatg aatgacttat ttataaaaaa gtttaataat gaagaaataa taactcttat
     6601 acttgataat agaatttgtt ggattgctaa agatgttgct aaaatactta attatgacga
     6661 tccctctaaa gctattaatc agtgtataaa ggcagaaaag tttgaatatg gaatagagta
     6721 tgaggtttta gctggagata agttaaaaga agttaaaaga cttattggtg taacacacat
     6781 aagttattta aagcaaactc caaagttagt tatattttat gaagaaggat tatatggatt
     6841 tataaattat agtaaacttc ccatagggat tagttttaga aaatggttaa gaagagaggt
     6901 attacctgaa cttagggcta aaggaactta ttctattaat aaagaatctt ataaagataa
     6961 cttaaaatat aaa
//
LOCUS       DAEVGI010000131         6953 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEVGI010000131 DAEVGI010000000
VERSION     DAEVGI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.131"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            472..2763
                     /locus_tag="K8M77_002062"
     CDS             472..2763
                     /locus_tag="K8M77_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBI7086532.1"
                     /translation="MSDNKILTYEREVLSETKRWIESEEERLEGEKLKANEKLDALNK
                     KSKGAYDPEIEIVKKSVDFLSKEEEKFKEAYNKPYFARIDFREYRKDFESFYIGKCGL
                     GDMLEGEEKVIDWRAPLADLYYSGTQGEAYYKAPIGVIEGELSLKRKFLFDHEHNLKE
                     CFDEGINEIILKSGLNEEGDGLIDEFLRINLENSTGTKLKDVVATIQKEQNDIIRAPK
                     NSPLIIQGSAGSGKTTVALHRLAYLLYRYKETITGEDILVVAPNKIFLDYISEVLPNL
                     GVDKVEQKTFEELALKKLGIKGKVITKDKKLVEILENDLEKNKYIMNDSKIKGSILFK
                     EMLDRYIQIIEREDSHIDNIRICGYSVFDSKEIKRLFLKDLIKYPVNKRKDEIKNYFS
                     KKLGEKVSKVLDKVDFQYEYKIARLKKSMEDSKERREKLIELYDERDNHKKEIKKSAR
                     KEFNEYFNRWKGIDTTNVYKNFFENKEIFNEVTGGKIPDKLAEYMRENLANNIDKKII
                     DSDDLAAMLYLKFRIEGIEESSLLKHIVIDEAQDYSMLQLYVMSMIASGKSLTIVGDI
                     GQGIYSFKGINDWKEAIKLVYDENAEYKHLSQSYRSTVEIINYANKVLVKQNTYEEPA
                     KPVLRHGMVPEEIKFNNAKEFCERLDNIVKKVKDEGKTSIAIVGKTLDECKKIRNLVK
                     KYSNYDFELIKDDQKEFDLNLIIIPSYLTKGLEFDCTIVYNLNKENYNDNEIDKKLLY
                     VVLTRALHYEYIFYKEEKSPLIG"
     gene            2780..3037
                     /locus_tag="K8M77_002063"
     CDS             2780..3037
                     /locus_tag="K8M77_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBI7086533.1"
                     /translation="MEEKQIMAFRDEEGNKVEFEVVAKIYLGEKNKKEYIVLSPVEGN
                     GDEADDFVFRVDKVNDSVEYNLVEDDEEFRLVKKEYKKLLY"
     gene            3053..3448
                     /locus_tag="K8M77_002064"
     CDS             3053..3448
                     /locus_tag="K8M77_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086534.1"
                     /translation="MEKYRIINFLSTNGLTEIEEVSSEENRLALKFYYDFEDVEIQGA
                     KACANDETDYEEDSNPWIVEGVIPYLDDIATDSVEEILEEVMEDFEIIAKVEGYEIEA
                     SNYSGKQFLAVFLPEDEELEIDDILDELK"
     gene            3559..3754
                     /gene="ssrS"
                     /locus_tag="K8M77_002065"
     ncRNA           3559..3754
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="K8M77_002065"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3801..3989)
                     /locus_tag="K8M77_002066"
     CDS             complement(3801..3989)
                     /locus_tag="K8M77_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086535.1"
                     /translation="MSKSAWDYTLEILSLMGDIGYYNDLLSKNLNKKEREVYSKKIDS
                     LESKFFSLKEKLKNTSIF"
     gene            4141..4353
                     /locus_tag="K8M77_002067"
     CDS             4141..4353
                     /locus_tag="K8M77_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086536.1"
                     /translation="MSREISNKDRENIVRRIYERMKHDFKEEEFYAEVSLSEMEDSIK
                     KHYLKGKSNFKTLEELIDDEIKNEKF"
     gene            4559..5854
                     /locus_tag="K8M77_002068"
     CDS             4559..5854
                     /locus_tag="K8M77_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI7086537.1"
                     /translation="MNKRKLTAIILSISMIFAVSINSNKIVQAKTTEEAQQEINSNKS
                     KIDDLKDKQNDINSEKSKSQSKLDEIQKQIADKNQKLLTSQKKVDEYKANIDSLKDSI
                     DKLQGQINDIQINMDKKKKEEKEKEIILSGRIRSAYKSNLSNQFLYIMLESKNVGDFI
                     SNVSSIKYVVDRDNKLIDDIKKVQDELKSEESQLKSQEEELSSKKTKLENEKKEYDNL
                     VSQYQSQLNELNSLEEQKQAEINSLSEKERAVLDEINSYEEDNANLKDYINNLINEKK
                     SVKLNSDNNSTTSTSNNEVSSEQKSNSSSGFMRPAPGGVTDPFGPRVHPVTGKRSVHT
                     GADLGAAYGTPILASKSGTVVEAGWNTAYGNMVIIDHGDGTSTLYGHSSRLAVQAGQH
                     VSQGQVIAYVGSTGYSTGPHLHFGIMINGQWVNPMNYIN"
     gene            5982..6347
                     /locus_tag="K8M77_002069"
     CDS             5982..6347
                     /locus_tag="K8M77_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086538.1"
                     /translation="MKALIWIIIILVAVGLLFESMYKIKMVFENFSKNNNRKAGKDFN
                     FDFKNQFNNIPEEKKEEFIRSLSDDKESLFKEYLACNKEDENLLREKLDEKHVFNIFN
                     RWARQNNIEPINISNYKEL"
     gene            complement(6415..>6953)
                     /locus_tag="K8M77_002070"
     CDS             complement(6415..>6953)
                     /locus_tag="K8M77_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI7086539.1"
                     /translation="SSGYFAPLIYIIAFSLVPLTFFPDSVLAILGGSIFGLGGGFLYT
                     SIGALIGGSISFFISRILGQSFVEKFENDKIKNIQELLKDNGFLMILLLRLIPLFPFD
                     LISYGAGLTKISYKDFALGTLIGTIPGILVFVNLGAQWITFNKTSIYLSVSLLILFII
                     LSVFLKKIYINKHLEKGN"
BASE COUNT         2914 a          614 c         1216 g         2209 t
ORIGIN      
        1 aaaaaattta tttttaataa gaaattctta ggttaatatt ttaagagggg cttttaaaaa
       61 aattaattaa taagtttatg attttaagtt atattcatag attgtgtaat agaaaatata
      121 attgaactta ataataaaat ctaatataat taacaatatg atgtaggcaa gttataataa
      181 tgactttatg aaggaaaata aattgattat acctaaaaat atgttctatc tttaatataa
      241 ttttacataa atgaattagt ttatataaga atttaaatga ttaaaacata tttcgtgaaa
      301 taaaggtgta aaaggactta cattgttagt aaataataat gaagtattct tttacacctt
      361 ttttaaggtg tattctagaa attaaaattt tttgttctat aaattatttt ttattattta
      421 tgatttaaat gagaaattaa atgggactat ttagtgaggg aggtatttat tatgagtgat
      481 aataagattt taacttatga gagagaagtc ctttcagaaa ctaaaaggtg gattgaaagt
      541 gaagaggaaa gattagaagg tgaaaagtta aaagctaatg aaaaactaga tgctttaaat
      601 aagaaatcta agggagcata tgatccagaa atagaaatag ttaagaagtc tgtagacttt
      661 ttatcaaagg aagaagaaaa gtttaaagaa gcttataata agccatactt tgcaagaata
      721 gattttaggg aatatagaaa agattttgaa agtttttata taggtaaatg cggacttgga
      781 gatatgctag aaggagaaga aaaggtaata gattggagag cacccctagc tgatctttat
      841 tatagtggta ctcaaggaga ggcatattat aaggcaccta ttggagttat tgagggtgag
      901 cttagtttaa agagaaaatt tctttttgat catgagcata acttaaagga gtgttttgat
      961 gagggaatta atgaaattat tttaaaatct ggtcttaatg aagaagggga tgggttaata
     1021 gacgaattcc ttagaattaa tttagaaaat agcacaggga ctaaattaaa ggatgtagta
     1081 gctactatac aaaaagagca gaatgatata ataagagcac ctaaaaattc acctttaata
     1141 atacaagggt cagcaggttc aggaaaaacc actgtagcac ttcatagact ggcttattta
     1201 ttatatagat ataaggaaac cataactgga gaagatattt tagttgtagc gccaaataaa
     1261 atatttttag attatatttc tgaagtgttg cctaacttag gagtagataa ggttgaacaa
     1321 aaaacttttg aggaattagc cttaaaaaag ttaggtataa aaggtaaagt tataactaag
     1381 gataaaaaat tagtagaaat tttagaaaat gatttagaaa aaaataaata tataatgaat
     1441 gatagtaaaa taaaagggtc tatattattt aaagaaatgt tagatagata tattcaaatt
     1501 attgaaagag aagatagcca tatagataat ataagaattt gtggatattc agtttttgat
     1561 tcaaaggaga taaaaagatt attcttaaaa gatttaataa aatatcctgt taacaagaga
     1621 aaggatgaaa tcaaaaacta tttttctaaa aaattaggag aaaaggtaag taaggtttta
     1681 gataaggttg attttcagta tgagtataaa atagctagac ttaaaaaaag tatggaagat
     1741 tctaaggaaa gaagagaaaa acttatagag ctttatgatg agagagataa tcataaaaaa
     1801 gaaataaaaa agtctgctag aaaagaattt aatgaatatt ttaatagatg gaagggtata
     1861 gatacaacta atgtttataa aaatttcttt gaaaacaagg agatttttaa tgaagtaaca
     1921 ggggggaaaa taccagataa gttagctgaa tatatgagag aaaatttggc taataatata
     1981 gataaaaaaa taatagattc agatgactta gcagctatgc tttatttaaa atttagaata
     2041 gaaggaatag aggaaagcag cttattaaaa catattgtca ttgatgaggc acaggactat
     2101 tctatgctac agttatatgt tatgtctatg atagcttcag gaaagtcttt aacaatagtt
     2161 ggagatattg gtcagggaat atattcattt aaaggaatta atgattggaa agaggctatt
     2221 aaattagttt atgatgaaaa tgcagagtat aagcacttaa gccaaagcta tagaagtaca
     2281 gtagagataa taaattatgc aaataaggtt ttagttaaac aaaataccta tgaagagcca
     2341 gcaaagccag ttttaagaca tggaatggtt ccagaggaaa ttaagtttaa taatgcaaaa
     2401 gaattttgtg aaagattaga taacatagtt aaaaaagtaa aagatgaagg aaaaacttca
     2461 atagctatag tgggtaaaac ccttgatgaa tgtaagaaga taagaaactt agttaagaaa
     2521 tattcaaatt atgactttga attaattaaa gatgatcaaa aagagtttga tttgaactta
     2581 ataattatac catcatatct tactaaagga ttagaatttg actgtactat agtttataat
     2641 ttaaataagg aaaattataa tgataatgaa atagataaga aacttcttta tgtggtttta
     2701 actagagcgt tacattatga atatatattt tataaagagg aaaaatcacc tttaataggt
     2761 tgatttagga ggagaaaaaa tggaagaaaa acaaataatg gcttttagag atgaagaagg
     2821 aaataaagtt gaatttgagg ttgttgctaa aatatattta ggagaaaaga acaaaaaaga
     2881 atatatagtt ctttcaccag tagaaggtaa cggagatgaa gctgatgact ttgtttttag
     2941 agttgataaa gtaaatgatt cagttgaata taacttagtt gaagatgatg aagagtttag
     3001 attagtaaaa aaagaatata agaaattatt atactaaaaa gaggtaatta ttatggagaa
     3061 gtatagaata ataaattttt tatctactaa tggattaaca gaaatagagg aagtttcttc
     3121 agaagagaac agattagctc ttaaatttta ttatgatttt gaagatgtag aaatacaagg
     3181 tgctaaagct tgtgcaaatg atgaaacaga ctatgaagaa gatagtaatc catggattgt
     3241 ggaaggtgtt attccatatt tagatgatat tgcaacagat agtgttgaag agattttaga
     3301 agaagttatg gaagactttg aaataatagc taaagttgaa ggatatgaaa tagaagctag
     3361 caattattca ggaaaacagt tcttagctgt gttcttgcca gaggatgaag aattagaaat
     3421 agatgatatt ttagatgaat taaaatagtt tttagaatag ttttaagttt atacttaact
     3481 aaaattatct caatagtaaa aacattctaa aaattcaatg ttgctttttt tatatcttgt
     3541 gttataataa aagcataatt aatcctgaga tgtacgcgaa gcttattaat tttaacctac
     3601 atgagaaata cgggatttgc aaaacagcta atgaaatcgg gcttcaagtt ttacttcact
     3661 tttgtgacgg aagatgatag agttagtttg cgtaaaaccc acctgcgaga ggcaggtata
     3721 aaattgtaag tgtgacggca tatttgggaa cattaattga gtttgtttaa aggttcaact
     3781 tttgttggac ctttttttca ttaaaaaatt gatgtgtttt ttagcttttc ttttaaagag
     3841 aagaacttac tctctagtga gtctattttt ttagaataca cttctcgctc tttcttgtta
     3901 agatttttac ttaacaaatc attgtaataa ccaatgtcac ccattaaaga tagaatttct
     3961 aaggtatagt cccaagcaga tttactcata aaaaacctcc taagtttaaa atctagtaat
     4021 agtatagata ttaatacaag agttggtgaa taattttaat taattacttt aatatatttt
     4081 agtccatgtg tataatataa ttatgaataa attattaact tgcataggga gtgaatggtt
     4141 atgagtagag aaataagtaa taaagataga gagaatatag taagaagaat atatgaaaga
     4201 atgaaacatg atttcaagga agaagagttt tatgctgaag tttcattatc agaaatggaa
     4261 gatagtataa aaaaacatta tttaaaagga aaaagcaatt ttaaaactct agaagaactt
     4321 atagatgatg aaataaaaaa tgaaaaattc tagtttataa ttaaggacta tataaaagca
     4381 tgttacttaa gaaaagtatg cttttatata gtctttttaa aagtaaattt aataagggga
     4441 taattaaatt taacatatat atttattata ttaacaaaat tatttttatg gtgtataata
     4501 tgttgatgta tggtgtttta gtaaaataat atatttaaat aagaaatagg tgaaatatat
     4561 gaacaagaga aaattaactg caattatact tagtatatca atgatttttg cagtttcaat
     4621 aaatagcaat aagattgtac aagctaagac tacggaagaa gctcaacaag aaattaatag
     4681 taataaaagt aaaatagatg atcttaaaga taaacaaaat gatataaatt ctgagaaaag
     4741 taaatctcaa agtaaattag atgaaataca aaagcaaata gccgataaaa atcaaaaact
     4801 acttacttca caaaagaaag ttgatgaata taaagctaat attgattctt taaaggatag
     4861 catagataaa cttcaaggac aaattaatga tattcaaata aatatggata agaaaaaaaa
     4921 ggaagaaaag gaaaaggaaa taatactttc aggtagaata agaagtgctt ataaatcgaa
     4981 tttaagtaat caatttttat acataatgct tgaatcaaag aatgtagggg actttataag
     5041 taatgtatca agtataaaat atgtggtgga cagagacaat aaattaattg atgatataaa
     5101 gaaagttcaa gatgagttaa aaagtgagga atctcaatta aaaagtcaag aagaagaatt
     5161 atcaagcaaa aaaactaagt tagaaaatga gaagaaagaa tatgataatt tagtaagtca
     5221 gtatcaatct caactaaatg aattaaattc cttagaagag caaaaacaag ctgagataaa
     5281 tagtttaagt gaaaaagaaa gagcagtatt agatgaaatt aatagttatg aagaagataa
     5341 tgctaatctt aaagactata taaataattt aattaatgag aaaaaaagtg ttaagttaaa
     5401 tagtgataat aatagtacaa ctagtacaag taataatgaa gtaagttcag agcaaaagtc
     5461 taattcttca agtgggttta tgagaccggc tccaggtgga gttacagatc catttggacc
     5521 tagagtacat cctgttacag ggaaaagaag tgttcataca ggggcagatt taggggcagc
     5581 ttatggaact cctattcttg catcaaagtc aggtactgtt gtcgaagcag gatggaatac
     5641 ggcttatggt aatatggtta taatagatca tggagatggt acaagtactt tatatggaca
     5701 ttcatctaga cttgctgtac aagctggtca acatgtatca caaggacaag taattgccta
     5761 tgtaggatca acaggatata gtacaggacc tcatcttcat ttcggtataa tgataaatgg
     5821 tcaatgggta aatcctatga actatataaa ttaatttaaa gggagtgcat taaaattata
     5881 ttacaaagaa aactgtgata gaatttcttt gaagttaaat tttaatatgc tcctttttac
     5941 ttaatatagt ttaaataaaa atttagtttg gagatgatta ggtgaaagct ttaatttgga
     6001 taataataat attagttgct gtaggcctat tatttgagtc aatgtataag ataaaaatgg
     6061 tatttgagaa tttttctaaa aataataata gaaaagctgg aaaggatttt aattttgatt
     6121 ttaaaaatca gtttaataat ataccagaag aaaagaaaga agagtttata agatccttat
     6181 cagatgataa agagagttta tttaaagaat acttagcttg caataaagaa gatgaaaatt
     6241 tattaagaga aaaattagat gaaaaacatg tatttaatat atttaacaga tgggcaagac
     6301 aaaataatat agaaccaata aatataagta attataaaga gttataaaag gtttttataa
     6361 tataaaaata gtccaaggat ttttcttcct aggactattt ttattttaaa agatttaatt
     6421 gcctttttct aaatgcttat ttatataaat tttctttaag aacacagata atattatgaa
     6481 taatattaat agacttacag atagatatat acttgttttg ttaaaagtaa tccattgagc
     6541 tcctaaattt acaaaaacta atatgccagg aatagttcct attaaagtac ctaaagcaaa
     6601 gtctttatat gatattttag taagaccagc tccatagctt attaagtcaa aggggaagag
     6661 cggtattaat cttaaaagta gaatcattaa aaatccattg tcctttaata attcttgtat
     6721 atttttaatt ttatcatttt caaacttttc tacaaaggat tgaccaagga ttcgagaaat
     6781 aaagaaagat atacttccac ctattaaagc accaatactt gtatataaga atcctccgcc
     6841 tagtccaaat atacttccac ctaagattgc taatactgaa tctgggaaaa aagttaatgg
     6901 aactaaggaa aaggctatta tataaattag tggagcaaaa tatccagaac tta
//
LOCUS       DAEVGI010000132         6919 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEVGI010000132 DAEVGI010000000
VERSION     DAEVGI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.132"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..938)
                     /locus_tag="K8M77_002071"
     CDS             complement(<1..938)
                     /locus_tag="K8M77_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBI7086540.1"
                     /translation="MRFIGVRTWKTGIGAVIAMIIAKELGLSYWVSAGIITILSIQST
                     KRESLKIAIKRIESVIIALIVSSVLFLALGFNSVVFGLYILVFIPLTVKLKVTDGIVV
                     SSVLVTHLLVEKQVNSYWIINELGLMLVGVLVALILNSYMPKNEEKIKEDIDYISEKI
                     KEIFMDMAYSLRTHSVSIKEQRLFDELENRIELAKKRANDNFNNYLFSDVKYYVHYID
                     MREVQFQILKYMREHFSRISITVKQTELVANFTEEVASVIGKEVNVNILINRLNRLKK
                     ELSEQELPVTREEFENRAMLFQFINDLEIFIKTKNQ"
     gene            1086..1727
                     /locus_tag="K8M77_002072"
     CDS             1086..1727
                     /locus_tag="K8M77_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HBI7086541.1"
                     /translation="MRIPKHIGVIPDGNRRWAVENGLEKDQGYLNGIKPGLELFKLCQ
                     KLDIKEITYYGFTTDNTKRPSYQKKAFIDACIKSVELIANEDCELLVIGNADSPVFPE
                     DLKKYTKRTTIGKGGIRVNFLVNYGWEWDLNLLKACDIKSKNIYDNIQSKDISRIDLL
                     IRWGGRRRLSGFLPVQSVYSDIYIIDDLWPNFNKDHFFNALEWYKDQDITLGG"
     gene            1732..3229
                     /gene="crtI"
                     /locus_tag="K8M77_002073"
                     /pseudo
     CDS             1732..3229
                     /gene="crtI"
                     /locus_tag="K8M77_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476382.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
     gene            complement(3261..4952)
                     /locus_tag="K8M77_002074"
     CDS             complement(3261..4952)
                     /locus_tag="K8M77_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI7086542.1"
                     /translation="MIRDVNDNKKKKVDEILSIVKVSSLLFSAMAFLQYTFNLTKYNN
                     KFLAYYSILSILISIFLLSTIYILWSLASLNKYKDHINKIQLIEIILFMIIFIMAILV
                     SGGNSSNNKFLFLFIIITSTIQGGMHRGCVVSVVSSAIILAIDLIFSGNADVNIYFQN
                     DLILAGVFVLTAWTLGFYVKTENDHIKELQTLVNEDGLTGVYNHRYFYDKLREKMHIS
                     EVENEKLSLIFMDIDYFKHYNDLYGHQKGDEALKKIGKLLKNIARENDLVARYGGEEF
                     AIILPNTSEEEATKIAERIRNKVEKTYFEGQENQPNANFTVSVGIAAFPDKAKSELEL
                     VKCADDALYRAKFFHKNRVETYVSVLDELKRDLNEEENTLVTSIKTLISIINAKDKYT
                     YSHVERVVIYSRLMAKKLELSEDEKKQLIYGAYMHDIGKINIPNEILTKKMRLTKEEW
                     EILKQHPKDGVEIIKSVNSLKNVVPLILYHHERYDGFGYPVGLKEKEIPYLARVLTVI
                     DSFDAMTSSRPYNKRKTYDEGLIELERCSGTQFDPEIVRIFSEVVKENINEIQDF"
     gene            complement(5012..5146)
                     /locus_tag="K8M77_002075"
     CDS             complement(5012..5146)
                     /locus_tag="K8M77_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBI7086543.1"
                     /translation="MKKLNKNLLTLFAALTTVVATTVATSACLWFTHQPEEPKSLRDE
                     "
     gene            complement(5167..5811)
                     /locus_tag="K8M77_002076"
     CDS             complement(5167..5811)
                     /locus_tag="K8M77_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HBI7086544.1"
                     /translation="MIENLSKLISEKVSSELNYDNERKEIIQYGTYALIQTLISIISV
                     LILGLVFNIALEALIFLFTASILRKYSGGAHSESSNVCTLLGIIISICIGFLIKSSFF
                     AKMNFELVVFIGIVIFVFGYFIVFKFAPVDTKNKPIKTEKKKKRMKKGSLKILTIYLF
                     IEVLSIILYYNSGWSLAKPVMLSIIFGVAWQCMTLTYIGNILLKTIDSFTNKLL"
     gene            complement(5813..6274)
                     /locus_tag="K8M77_002077"
     CDS             complement(5813..6274)
                     /locus_tag="K8M77_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086545.1"
                     /translation="MLKVTLFETLVRGIPEALAMMLAMYAFANKKLEKKEYLISSLIL
                     VLVVYLIRLLPINYGIHTILNIFVLIFLAFNVNKIDLIVSIKASILILMILFLCEGLN
                     LLFIEKFFSENIDYLFENVFTKTILGIPSTIIFMVIVTYYYLIASKKGKLR"
     gene            complement(6291..6857)
                     /locus_tag="K8M77_002078"
     CDS             complement(6291..6857)
                     /locus_tag="K8M77_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HBI7086546.1"
                     /translation="MIEAVIIALIISKIKGYSLKPFFKSWTIYPILIFEVIYIVFQIS
                     IFMGNYDVVKWAPWFKSIYMYLFLIPIFWYKEYVSALIGSLFILAGTFLNHIVMAANG
                     GEMPVFPSLSYLTGYLKPNTILKVNDIHIIGTSTVKYKYLSDIIDIGYSVLSIGDILI
                     RIFVVIIIYVTVKKTNEKNEYKQSNIFA"
BASE COUNT         2506 a         1060 c          747 g         2606 t
ORIGIN      
        1 cattgatttt ttgtttttat aaaaatctct aaatcattaa taaattgaaa taacatagct
       61 ctattttcaa attcttctct ggttactgga agttcttgtt cacttaactc ttttttcaat
      121 ctatttaatc tatttataag aatatttacg ttaacttctt tacctattac agaggctact
      181 tcttctgtaa aattagcaac tagttcagtc tgttttacag taatagatat tctagaaaag
      241 tgttctctca tgtactttaa aatttgaaat tgaacttctc tcatatctat ataatgcaca
      301 tagtacttaa catctgaaaa caaatagtta ttaaaattat cattagctct ttttttagct
      361 aactctattc tattttcaag ttcatcaaaa agtctttgtt cctttattga aacactatgt
      421 gttcttaaag aatatgccat gtccataaat atctctttta ttttttcaga tatataatct
      481 atgtcttctt ttatcttttc ctcattctta ggcatataac tatttaatat taatgcaact
      541 aaaaccccaa ctagcataag acctagttca tttattatcc aatatgaatt cacctgtttc
      601 tcaactaata aatgagttac taaaacagaa cttacaacaa ttccatccgt cacttttagt
      661 tttactgtta aaggtataaa aactaaaata tataatccaa aaacaactga gttaaaccct
      721 aatgctaaga ataaaactga agacactatt aaggctataa taacagattc tattctcttt
      781 atagctatct ttaatgattc tctttttgta ctctgtatgc taagtatggt tataattcct
      841 gctgaaaccc agtaacttag ccctaattcc tttgctatta tcatagctat tacagcgcct
      901 attcctgttt tccaagttct aacacctata aatctcattt tttcaactcc gtataattaa
      961 cattaaattt ttccataaaa taataatata attaaaattt aaaataatca aatttatgca
     1021 tgttatttat taaaatggga atactttcat ttgaatctat ttttaagaaa gggttgagac
     1081 atattatgag aattccaaaa catatagggg ttatcccaga tggcaataga cgttgggccg
     1141 tagaaaatgg acttgaaaag gatcaaggat atttaaatgg aattaaacct ggacttgaac
     1201 tttttaaact ttgtcaaaaa ctagatatta aagaaataac ttattatggt tttactacag
     1261 acaataccaa aagaccatca tatcaaaaaa aggccttcat agatgcctgc ataaaatcag
     1321 tggaattaat tgcaaatgaa gattgtgaat tgcttgttat aggtaatgct gattctccag
     1381 tttttcctga agatttaaaa aaatatacta aaagaactac aataggcaaa ggtggcatac
     1441 gcgttaactt ccttgttaac tatggttggg aatgggattt aaaccttcta aaagcctgtg
     1501 acattaagag taaaaatatc tatgataata ttcaatcaaa ggatatttca agaatagatt
     1561 tattaataag atggggcggt agacgtagat taagcggatt tttaccagtt caatcagttt
     1621 attctgatat atacataatt gatgatttat ggcctaattt taataaagat catttcttta
     1681 atgcccttga atggtataaa gatcaagata ttaccctagg tggttaatta aatgagtaaa
     1741 aataaaaaag ctataatagt tggagctgga ataggtggat tagctactgc tgttcgcctt
     1801 cttattaata attttgaagt agatattttt gaaaaaaact ctaagatagg tggaaaagta
     1861 aatttaattg aatacaaaga ttttaagata gattcttctg cttcaatatt tatgctccct
     1921 aagccctatt tagaagtatt taaatatgca aaaaaagacc ctaaagacta tatagaactt
     1981 gtagaattaa atactttata taaagtgttt aatgatgaag gggatagttt taatatttat
     2041 tccgactttt taaaaactac agagtcctta gaaaaagtat ttaatgatga aagttcaaat
     2101 tattataaat atatatctga ctcctataga agatatcttt tagtagaaaa atacttttta
     2161 aataggagtt tttttatctt aaattattgg agatatttaa aatctttacc tgaactaatt
     2221 aaaatacatc cttttaaaag ttgttataaa accattgaaa aatatgtaag taatgaatat
     2281 ttaaaaacct tattagcttt ccaatgcatg tatcttgggg agtctcccct taaaagctct
     2341 aatgttttta atttaattcc atcaaccact caaatatatg gattatatta tattaaaggt
     2401 ggaatgtact cttacgttaa ggctttagaa aaattaatcc tagaacttgg tggaaaaata
     2461 catcttaact caaatgtaac taatatactt atggaaaaga atgtaacaat tggcgcaaaa
     2521 ataaaccacg aaaatatgtt ttctgatcta atcgtttgta attcagattt tacttatact
     2581 atacaaaatt tacttcctag aagtacattt aaaaataaaa tttctaaaag aaaacaaaat
     2641 aatttatcct tttcttgctc tacatttata ctacatttat ttcttaagaa aaaatacaaa
     2701 aatttacatg tacataatat agtacttaat ttaaataaga aagaagtttt attagctccc
     2761 tttatagatg ggcccttacc aaaggaatat atattatatc tattgcccaa gctcaataga
     2821 tacttcatta actcctccgg attgtgaatg cattaatata acagtacgtg ttccaaacta
     2881 aaaaaaatat aaatcaaaat ggactgagtc tacaattgtt tctttgagaa acaaaatctt
     2941 atatgaccta agtaaaatta aaggtttaga agatataaaa gaaaatataa tttacgaaag
     3001 ctatacaacg cctatgacct taaaaaatga ttttaattgc ttttttggtg cagcttttgg
     3061 gcttaatcat aatttgctac aaacaactat ttttagacct caggcaaaaa taaaaaaact
     3121 aaaaaatata tattttgtag gtgattcagt acatcctggc tctggaatat caatgtcttt
     3181 aacctcagct aagctatgct gtgaaaaaat aatatcagat tttagctaaa ataaaaagag
     3241 gtgtattatg atacatctct ttaaaaatct tgtatttcat ttatattttc cttaaccact
     3301 tcactaaaaa tcctaactat ttctggatca aattgagttc cactgcatct ttctagttct
     3361 attaatcctt catcataagt ttttctttta ttataaggtc tacttgaagt catagcatca
     3421 aaactatcta ttacagttaa tacccttgct aagtatggta tttctttctc ctttaatcct
     3481 actggatatc caaagccatc atatctctca tgatgatata aaataagagg cactacattc
     3541 tttaaggaat ttactgattt tattatttct actccatctt taggatgttg ctttaaaatc
     3601 tcccattcct ctttagtaag tctcatcttt ttagttaata tttcatttgg tatatttatt
     3661 ttacctatat catgcatata agctccatat attaactgct ttttctcatc ttcggaaagt
     3721 tccaattttt tagccataag tctactataa ataactactc tttctacatg gctatacgta
     3781 tatttatctt ttgcatttat aatacttatt aaagttttta tggatgtgac aagagtattt
     3841 tcttcttcat ttaaatctcg ttttaattca tctaaaacag atacataagt ttcaactcta
     3901 tttttatgga aaaatttagc tctatataat gcatcatcag cacatttaac taattctagc
     3961 tcacttttag ctttatctgg aaatgcagct ataccaacag atactgtaaa attagcatta
     4021 ggttgatttt cttgtccttc aaaatatgtt ttttctactt tatttctaat tctttctgca
     4081 attttagtag cttcctcttc tgaagtatta ggtaaaatta ttgcaaactc ttctcctcca
     4141 tatctagcta ctaaatcatt ttctctagct atatttttta gaagttttcc tattttcttt
     4201 aatgcttcat cacctttttg atgtccatac aaatcgttat aatgcttaaa ataatctata
     4261 tccataaata ttaatgacag tttctcattt tcaacctcac taatatgcat tttctctctt
     4321 aatttatcat aaaaatatct atgattataa acccctgtta atccatcttc gttaaccaag
     4381 gtttgtaatt cctttatgtg atcattttca gtttttacat aaaaacctaa tgtccaagct
     4441 gttaaaacaa atactcctgc taatattaaa tcattttgaa aataaatatt tacatcagca
     4501 ttccctgaaa aaatcaaatc tatagctaaa ataatagctg atgacactac agaaaccaca
     4561 caccctctat gcatgccccc ctgtattgta gatgttatta taataaataa aaataaaaat
     4621 ttattattac tagaattccc tcctgaaact aatatagcca ttataaatat aatcataaat
     4681 aaaattattt ctattaattg tatcttattt atatgatctt tatatttgtt taaactagct
     4741 aaactccaaa gtatgtaaat cgttgataat aagaatatac ttattaaaat tgataatata
     4801 ctgtaatacg ctaaaaattt attgttatac tttgtaagat taaaggtgta ttgtaaaaaa
     4861 gccatagctg aaaataataa tgatgatact tttactattg ataatatttc atcaactttt
     4921 ttctttttat tatcatttac atccctaatc atattctccc tctctaatct agtataaaat
     4981 aaggccggtt taaaaccgac ctttaggtaa attattcatc tcttaaagat tttggttcct
     5041 ctggttggtg tgtaaaccaa agacatgctg aagtagcaac tgttgttgca actactgttg
     5101 ttaatgcagc aaataatgtt aataagtttt tgtttaattt tttcatttaa ctcatcccct
     5161 caagatttat aataatttat ttgtaaaaga atctatagtt tttaacaata tatttcctat
     5221 gtaggttaga gtcatacatt gccatgctac tccaaagatt atacttaaca taacaggctt
     5281 agctaaactc catcctgaat tataatataa aattatactt aatacttcaa taaacaaata
     5341 tatagttaaa atctttaaag atcctttttt catgcgtttt ttcttctttt cagtttttat
     5401 tggtttattt ttagtatcta ctggagcaaa tttaaaaact ataaaatatc caaaaacaaa
     5461 aataactatt ccaataaaca ctacaagctc aaagttcatt tttgcaaaaa aactactttt
     5521 tattaaaaaa ccaatacata ttgaaattat tatgcctaat aaagtacata cattagatga
     5581 ttctgaatgt gctcctccag agtattttct aagtatgctt gctgtaaata aaaatatcaa
     5641 agcttctaaa gctatattaa acactaatcc cagtataagt actgaaatta ttgatattaa
     5701 tgtttgtatt agagcataag ttccatactg aataatttct tttctttcat tatcataatt
     5761 taattcacta gaaacttttt cagatattaa cttagataaa ttctcaatca ttttatctca
     5821 actttccttt ttttgatgct attaaataat aataagtaac aataaccata aatattatag
     5881 tagatggaat acctaatatt gtttttgtaa atacattttc aaataaataa tcaatatttt
     5941 cactgaaaaa tttctctata aataataaat ttaaaccttc acataaaaat aaaatcatta
     6001 atattaatat tgatgctttt atacttacta ttaaatctat tttattaaca ttgaatgcta
     6061 aaaatattaa aacaaatatg ttcaatattg tatgaatacc ataatttata ggcaataacc
     6121 taattaaata aactactaat accaatatca aacttgaaat taaatattcc ttcttttcta
     6181 actttttatt ggcgaaagca tacatggcta acatcatcgc aagagcttct ggtattcccc
     6241 ttactaaagt ttcaaacaaa gttactttta acattcttcc tccactgtca ctaagcaaat
     6301 atattgcttt gcttatattc atttttctca ttagtttttt taactgtaac atatattatt
     6361 atgactacaa aaattcttat aagtatatct cctatactta aaacactata tcctatatct
     6421 attatatcac ttaaatactt atattttact gtacttgttc ctataatatg tatatcattc
     6481 acctttaaaa tagtgttagg cttaagatat cctgttaaat atgataaact tggaaaaacg
     6541 ggcatttccc caccattagc agccattaca atatgattta aaaaagttcc tgctaagata
     6601 aacaaggatc caattaaagc acttacatac tctttgtacc aaaatatagg tattaaaaac
     6661 aagtacatat atatgctttt aaaccaaggt gcccatttta caacatcata gtttcccata
     6721 aatatactta tctgaaatac tatataaata acttcaaaaa tcaaaatagg atagattgtc
     6781 caacttttaa agaaaggttt taaactatat ccttttattt ttgaaattat taatgctata
     6841 ataactgctt ctatcattat tttactcctt taattaattg taaagtttta acaaattatt
     6901 aaaactttat ccatttaaa
//
LOCUS       DAEVGI010000133         6887 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEVGI010000133 DAEVGI010000000
VERSION     DAEVGI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.133"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            186..650
                     /gene="greA"
                     /locus_tag="K8M77_002079"
     CDS             186..650
                     /gene="greA"
                     /locus_tag="K8M77_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBI7086547.1"
                     /translation="MNNYLTEENVRKLREELDYRMSVVRAKIAKEKLEAAAHGDRSEN
                     AEYKEACRNYRENDNRIQYLLTMISTAIIVDEESNDKSTLGLNSKARIKFVEDDDEDI
                     VTLVTTMDLDLENMCISIESDLGKALMGKKVGDVVEVEAPGGNYTVEVLEIL"
     gene            808..2571
                     /locus_tag="K8M77_002080"
     CDS             808..2571
                     /locus_tag="K8M77_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBI7086548.1"
                     /translation="MKKDTILSASIGSTDFHYLQKDYDEIKKLNLNTWNEVAWIGDEL
                     NSKIVMWTNSSPVNNVTLSSSDFINENGDLISSNNIKISWLKETLANIGRSNPSAPLE
                     PFPDIIHNSISLNIEKNKIASAWINIKIPRNAKPGIYNGSIEVTADELEKAYTFDYSF
                     EVLNLVQPLPRETNTQIEFWQHPYTIARYYKISKEDLFTEKHFKYLRENLKEYRDMGG
                     CGVIATIVHEAWNHQSYDSDPSMIKWRKNSYGTFEFDYSHFDKWIQLNIDLGILDPEK
                     SFGQIKCYSIVPWDNRIQYFNEATNKEEAINPNPGSDLWINIWTQFLTSFMSHLEEKG
                     WFNITYISMDERSIDDLKACVDLIESITNNSYEHFKISSAMNYESGNDYSLLDRIDDI
                     SIGLSHINHNSDDMKNMAKHRQELGLLTTIYTCTGDYPSSFTISDPSEGAFTMWYSLC
                     QNTNGFLRWSWDGWVENPLENVSYKYWEPGDPFLIYPAEKDSIGKTFYSTPRLEKLKE
                     GIRDINKAKYLMEKAPSLKNSIENLIYSLKRPNKGENAYGSAVAASKEDRDLTISEVK
                     RMKNDINNFAREFISLTMETL"
     gene            complement(2694..6760)
                     /locus_tag="K8M77_002081"
                     /pseudo
     CDS             complement(2694..6760)
                     /locus_tag="K8M77_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
BASE COUNT         2275 a         1243 c          906 g         2463 t
ORIGIN      
        1 tggctcaaag tccgtcacaa gtccgtcaca actccgtcac taaagcataa aaagctatat
       61 tttctccttt agataaatat aaaactctat aaatactctc ctatttccta cttaacatct
      121 ttaaaattta tgtagatgtt gtataatgat aaaataaaga attaagaggt aagggagatc
      181 ttattatgaa caattatttg acagaggaaa acgttagaaa gttaagagaa gagttagact
      241 atagaatgtc tgttgttaga gcaaagattg cgaaagagaa acttgaggcg gcagcccatg
      301 gggatagatc agaaaacgct gagtataagg aagcttgtag aaactataga gaaaatgata
      361 atagaataca atacctttta actatgattt caacagcaat aatagttgat gaagaaagta
      421 atgataaatc tacactaggt cttaacagca aagctagaat aaaatttgtt gaagatgatg
      481 atgaggatat tgttacttta gttacaacta tggatttaga tttagaaaac atgtgtataa
      541 gcatagaatc agatttagga aaggccctaa tgggtaaaaa ggttggagat gtagtagaag
      601 ttgaagctcc tggtggaaac tatactgtag aagtattaga aatattataa tagacttagc
      661 ttaaagtgga taattttatt tgtccacttt ttttcttctt tatctaatct tttaacaata
      721 gacttttttt aaatgttcta tttttaaaac ttctatctta taatgtattt attactaatt
      781 tttagaaaaa taaggaaggt ttattttatg aaaaaagata ctattcttag tgcttctatt
      841 ggctctactg actttcatta tcttcaaaag gattatgatg aaataaagaa attaaactta
      901 aatacttgga atgaggtagc ttggatagga gatgaactta actctaaaat tgttatgtgg
      961 acaaactcct ctcctgttaa taatgttacc ctttcttcaa gtgactttat aaatgaaaat
     1021 ggggatttaa tctcttcaaa taatattaag atttcttggc ttaaagaaac cttagctaat
     1081 ataggacgta gtaatccttc tgctcccctt gaacctttcc cagatattat tcataattct
     1141 atttcactaa atatagaaaa aaataaaata gcctctgctt ggattaatat aaagattcct
     1201 aggaatgcaa aacctggaat ttataatggt tctattgagg tcactgctga tgaattagaa
     1261 aaagcctata cttttgatta ttcctttgaa gttttaaact tagtacaacc tcttccaagg
     1321 gaaacaaata ctcaaattga gttttggcaa catccttaca ccatagcaag gtattataaa
     1381 atatccaaag aagatttatt tacagaaaag cattttaaat atttaagaga gaatcttaaa
     1441 gaatatagag atatgggagg atgtggtgtt atagccacta tagttcatga agcttggaat
     1501 catcaatctt atgatagtga cccttcaatg attaagtgga gaaaaaactc ctatggcacc
     1561 tttgaatttg actactctca ctttgataag tggattcaac ttaatataga cttaggaatt
     1621 ctagatcctg aaaagagctt tggccaaata aagtgttata gtattgtccc ttgggataat
     1681 agaattcagt actttaatga agctactaat aaagaagaag ccataaatcc aaaccctggt
     1741 agtgatcttt ggataaacat ttggacacaa tttttaactt catttatgtc tcaccttgaa
     1801 gaaaaaggtt ggtttaacat aacttatatt tcaatggatg aaagaagtat agatgattta
     1861 aaagcttgtg ttgatttaat tgaaagtata acaaataact cttatgagca ttttaaaatc
     1921 tcttctgcca tgaattatga aagtggaaat gactactctc tcttagatag aatagatgat
     1981 atatcaattg gattatccca tataaatcat aattctgatg atatgaaaaa tatggctaaa
     2041 catagacaag aacttggatt attaactaca atatacacct gtactggaga ttatccaagt
     2101 agtttcacaa taagtgaccc ttcagaaggt gcttttacta tgtggtattc cctatgccaa
     2161 aacaccaatg gctttttacg ttggtcatgg gatggttggg ttgaaaatcc cttagaaaat
     2221 gtttcttata aatattggga acctggagac ccttttctta tatacccagc agaaaaggat
     2281 agcataggta aaacctttta ctctactcct agattagaaa aattaaaaga aggtataaga
     2341 gatataaaca aagctaaata ccttatggaa aaggctccaa gcttaaaaaa ttctatagaa
     2401 aatttaatct actctctaaa aagacctaat aaaggagaaa atgcctatgg atccgcagta
     2461 gcagcttcta aggaggatag agatttaact atctcagaag taaagagaat gaaaaatgac
     2521 ataaataact ttgcaagaga atttatttca ttaactatgg aaaccttgta gttaatctac
     2581 ttagaagttt tcacccttta gcaatcaata aaaatagacg tgtatcaaag taagttttat
     2641 tattatactt tgatacacgc cccttttata caacttattt tttatttaaa agattattct
     2701 cctaagatct tatccataac tatttggata tcagctttat ctactactcc atctttgttt
     2761 atatcagatt tttcacttaa agcatttcct gatataacct ctccttgata tttagatact
     2821 atggataaat ctcctacgtt tatagaacca tcctcattaa tatctgctga cccttcagat
     2881 tctctaatac ataatttaca gtatagatgt ttttagcacc agattctgaa cgagcaatga
     2941 ttattgattt attattataa actggtaata tagttacagc cacattttct tcccctgaag
     3001 cttctacagt tttaggtaat tcttttaaat taactgtgta ttcattagta tcagaattaa
     3061 atccttctaa ggcttctcca tctaatttta tttcagataa tttattacct tcattagcac
     3121 ttacttgctg tacatactca tatacagcaa cttctgataa tccaatacac ttacctactt
     3181 caggttgttg catataaaca cgaatactat cagtatttat tactttatct aagaaataag
     3241 ttgtttctcc agcaaggtat gatacctctg ttgtgtttga atgagaaact acctcatatt
     3301 caccagcttc atttttataa cttatttcta cctttttagg aattcttcca taaatattat
     3361 cggtaaataa ccataaatct aatctatcta tattatattt atttttccaa tctaattgaa
     3421 catatgcccc tttaggttca ccatttacag ttaaatttct ttcattccag tttgtccaac
     3481 gttctcctgt atttgaacta ttattagtta ttccattatt aatagaattt aagttatcag
     3541 ctgtagaaac gcaactttgg cttaaagcag gaacatcttt gttagaagaa ttatttatag
     3601 ctaagttagt agcagctttt aaagcttcaa ctacacgtac tgttgaagta acagtatatg
     3661 tcttacctaa taaggatact tctcctaaaa cttcaactgt tgtattaggc ttagaaaaat
     3721 ctactccttc taaattccaa tccactttaa actcttcact ttcatttcca tcagctaaat
     3781 atcctttaac agttttagga agtgtaggag tttgtccagc ataagtaaat gttgagtaat
     3841 tttccattga aaccacatca ccaattacat ggacatttac atttacagcc acatctgttc
     3901 cttctaattt accatttatc ttaaatactt gaggagtatt taattgtgat tcatcataat
     3961 cattccatga aatattaaaa gtctcagttg ttccatcact atatcttcct tctactgtac
     4021 ttggtaactc tggcttttca tttaagttaa catagtaatc tttaactatt tcataagatt
     4081 ctaagaactt atcttctcca gcattattta cagtattaat ttctatagat tgagattgta
     4141 atccctcacc tgaaacattt aatcttatat ttccagcatc cttagttgat tgaactatta
     4201 ctaaggcctt accacttaat gcttttctag aagcttctgt atcacttgta ggtttataag
     4261 aatctgtatc agtttgatct ccattatcaa cacctacaat ctttccattc ccttctaact
     4321 caaaatttaa tctattctct gcattttgaa ctatattgcc attttcatca acaatatcta
     4381 cagtaatata agataaatca tatccatctg aatcaatagt gtctctatct acacttaatc
     4441 ttacagtaga agcctcccca gttgttttta ctgtatttct tccctctgta tttgtgattt
     4501 catttccttc tttgtcatat gccttagcag ttaatgtccc ctctttataa ggaacattaa
     4561 agactggata taatgaatca tttccaaaag tatagtattt atatcctgca tcagttgtat
     4621 gctcagttga agtttgctcc ccaatctttt catcatttaa atataattca actttgtgag
     4681 catcagtaaa tacgtttact tcaacattcc cattttctat aactatatct tctctgttcc
     4741 atgttggaag tacatgtaat gtatttacat catcattcca ttgactttga tataaataat
     4801 aaatatcttt ttcaaatcct gcagtatcaa ctataccaaa gtaacttgat tttggagctg
     4861 ctccatttcc tccaccaact gcacctgtac cagtaccatt ccaaggagta ggttctccta
     4921 tatagtcaaa tcctgtccaa acaaattctc cagcattata atcatttttg attacaaact
     4981 tccaagcatc actagctgaa tgtccccaac caactcttgt ttgattatta tcatactctg
     5041 aaatacgatg attactataa tctattccct ttgttttata atatcctcta gtatgcactg
     5101 cactactagt ttcagaagca tataatgtcc aatctggatg gtttgctctt tgttgtgcaa
     5161 cctgtgcttc atttgcatag ttaaatccaa ctaatccacc attgtcatca accacctctg
     5221 aaatggcctc tgctgttcta tctccatttt tagttctatt atcacctata gtaacatgtc
     5281 ttgtttcatc ctcatcctta atccaatcta ttatattttg agctacgtta gtataattag
     5341 atgcacttcc actaattcct tctaatatct cattacctat tgaccacatt attatagatg
     5401 ggttattctt agagttttta accatagatc tagcttcaaa ctctccccat gtcatttctg
     5461 gagaaccatt taaaatttca ttatcttctc ctattacttc attaaaatac ttagaaaagt
     5521 catttacatt tccgttcttt ggatttgtcc atgtgtcaaa tgcttcatta ataactaaca
     5581 agcctaaacg attacaaatt tctaataaca tttcactagc agggttatga gaaacacgga
     5641 ttgcatttac tcccatttcc ttcattttct ccatttgacg ttctactgca cgataataag
     5701 atgccgctcc taaagcccct tggtcatggt gcatacaaac accttttaat ttcatatttt
     5761 ctccatttaa agagaatcca gtatctttgt taaaattata atatctaaaa ccaaaatcag
     5821 taaaataagt atctataaca tttccatcta ctaatacttc actttttacc ttatacatat
     5881 ttggattatc aactgaccat aaatctggat tatttacaat aacagtttga ttgaaagtat
     5941 aactttcatt agctcctata ctctgttcat ttattactga ctcgctaact tcttctccct
     6001 tagaatttac tactgtactt tttacagtaa ccatagaatt gtctttagac tcgttttcaa
     6061 taattgtctc aatagctaca tctacatctc cacctttttg ctcttcaagt tttggagtag
     6121 taactgttgt tcctgaatgg gcaacatgaa tagagtctgt tacatttaaa gttacatcac
     6181 gataaattcc acttccagaa taccatctac tagttggcac tggattagat accttaacag
     6241 caattatatt ttcagtttgt ccatcacatg ttaaagcttc agtaatatca aaggcaaaaa
     6301 aagtatatcc ataattatgc tcaccaacct ttttaccatt tacatatact tcagcattca
     6361 tatatactcc atcaaagttt aatgtaaaat ccttaccatt atacttttca ggaactacaa
     6421 aggctttacg ataccatcca gttcctccta ataagaatcc actttcagct tccccttcaa
     6481 cagtaaaatc tccttcaata ctccaatcat gaggtaagtt tagtcctctc catgaagaat
     6541 catcaaagtc taccccttca gcaccagaag gatctccttt aaaaaacttc cagttttgat
     6601 tgaatattgt tgatctctct ccatctttta tgaatccaac atttactgtt tcagattcag
     6661 gaatttgtct tgtttcagcc attactaagg taggcgctaa tgttaaagca aatgcaattg
     6721 ctgctcctaa agctgtcccc cttttgttaa atgattgcat attaatttcc ccccttttta
     6781 aatatacata taagctattt tacttatttt aacttatatg tataataata ccatcaagta
     6841 aattcatgat aaagtcataa aaacttttta actattgcaa aataatt
//
LOCUS       DAEVGI010000134         6839 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEVGI010000134 DAEVGI010000000
VERSION     DAEVGI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.134"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            40..423
                     /locus_tag="K8M77_002082"
     CDS             40..423
                     /locus_tag="K8M77_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HBI7086549.1"
                     /translation="MSNIKFCSIIIKDDFNNILISKRKGKKADEHLWYIFERKIKGRE
                     TPEKCANRAIKEDLKTIVFDLNKLCDLNVNEGETLRVFTGSLKEKITCGSNITTYKWI
                     SKDDLNDYTFANGELEKINAFFDTI"
     gene            486..704
                     /locus_tag="K8M77_002083"
     CDS             486..704
                     /locus_tag="K8M77_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086550.1"
                     /translation="MTLLRKKILGEDFHVTIKPIYNREDTLAFTFSPDQKKVEIGEEI
                     KITVKYPTEKYDAFCDELVELIARYQEI"
     gene            complement(737..1381)
                     /locus_tag="K8M77_002084"
     CDS             complement(737..1381)
                     /locus_tag="K8M77_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086551.1"
                     /translation="MKKEKFIPKLIFFLLGMAIIQLGVVIFLLSGAGSDPFTVFTQGL
                     SKALSVSTGTANMIILVTLVIAIFIADRKQLKVGTFVLVLVVGPTIDAIMGILHRFGL
                     DTSLSNFNLFTKCVIVAVSCIIIAIGFSFLVSTELGVAPNDLVPLIIRDKTNFQYRWI
                     RIGLDLSFLVVGYLLAGVVGLGTIIAVLFTGPAIQFFMPPCEKIVHKFIGKNAA"
     gene            1656..1988
                     /locus_tag="K8M77_002085"
     CDS             1656..1988
                     /locus_tag="K8M77_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086552.1"
                     /translation="MKDTKTKEHIARIAKASTYFIFRNGPVNKLHKENKVSDEELKEM
                     QEYMQNHLAYLYEVLLEEGNLKKYELVMNTMNQFYVNDDTEVVLADEGFDSLYDQLFP
                     KSSNIILK"
     gene            complement(2023..3243)
                     /locus_tag="K8M77_002086"
     CDS             complement(2023..3243)
                     /locus_tag="K8M77_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBI7086553.1"
                     /translation="MKNLSLIKRIFVAIILGILIGLGCSYINLDIPIRILMTFNSIFG
                     NLLSFLIPLIIVGFIVPGIASLGNKSGKGLFITTLISYASTFLIGILTFFIGRAVLPK
                     FIVSASLSTGSVNVDPYFTIDIPPMFGVMSALVFAFLLGIGISRIKNSYLLKVSEEFN
                     HVISLTIKNVLIPLVPIYILSIFSKLSYNGEIFTTLKSFGLVYLVLFSIQGSYLVVQY
                     ALAGTLKKENPLKLLKNMIPAYMTALGTQSSAATIPVTLNCTKENKVDQDVADFVIPL
                     GATINLAGDTITLVLASMSVMYMKGQVPTFSIMVPFIIMLGVTMVAAPGVPGGGVMAA
                     LGLLEGMLGFGNIEKSLMIALHAAQDSFGTATNVTGDGAIAIIVESILKKRNNTNIKI
                     EEAEEDFIPKVSCN"
     gene            3745..5214
                     /locus_tag="K8M77_002087"
     CDS             3745..5214
                     /locus_tag="K8M77_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI7086554.1"
                     /translation="MSQEQNTNLKRGLKSRHLSMIAMGGAIGTGIFLAMGDTLHQAGV
                     GGAFVAYGIIGVMVYFLCTSLGEMATYMPETGSFGAYATKFVDPALGFALGWNYWYNW
                     AITIAAEMVAGSLLMKFWFPNVPAIVWSALFLVIILTLNLLSAKAYGESEFWFAAVKV
                     ATVIIFLIVGVLMIFGILGGDNVGFKNFYENGGPFIGGAKSIFMIFLIAGFSFQGTEL
                     VGVAAGESEDPEKNVPKAVNTIFWRILLFYLGTILVIGALVPNAQAGVETSAFTIVFE
                     KLGLAGAASIMNAIILTSVLSAGNSGMYASSRMLYAMAKNGMAPKIFEKTNSRGVPVN
                     AIIITTIIASLCFLTGIYAENTVYVWLVAASGLSGFIAWLGIAICHYRFRKAYVAQGK
                     DMNRLVYKAKLFPLGPIIALILCGIIVLGQGYAYLDVRNGVDWGGLISSYIGLPLFFG
                     LFAYYKIKYKTKIIDLKDVDLSVEHIDTDESIEDEVFSY"
     gene            complement(5254..6359)
                     /locus_tag="K8M77_002088"
                     /pseudo
     CDS             complement(5254..6359)
                     /locus_tag="K8M77_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
     gene            6486..6704
                     /locus_tag="K8M77_002089"
     CDS             6486..6704
                     /locus_tag="K8M77_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HBI7086555.1"
                     /translation="MSDSIVKVKKNSNGEITDYVLRSGKTVSKAEGVKLAEEGMIDGV
                     IISHSKKGEAYLRSIPDGKESNNLDELS"
BASE COUNT         2492 a          929 c         1057 g         2361 t
ORIGIN      
        1 tgtatataaa aagcgaaatg tgtaaaaggg gagacgtata tgagtaatat taaattttgt
       61 tctattatta taaaggatga ctttaataat attttaattt caaaaagaaa aggaaaaaaa
      121 gcagatgagc acctatggta tatttttgaa agaaaaataa aaggaagaga aactccagaa
      181 aaatgcgcaa atagagcaat aaaggaagat ttaaaaacca tagtttttga tttaaataaa
      241 ctatgtgatt taaatgttaa tgaaggagaa actttaagag tttttacagg ttccttaaaa
      301 gaaaaaatta cttgtggctc taatataact acatataaat ggataagcaa agatgattta
      361 aatgattata cttttgcaaa tggagaatta gaaaagataa acgctttttt tgatacaata
      421 taaaaacatg ccaatattgg catgtttttc ttttattgtt ataataaaac ggtgaggtga
      481 ttattatgac attattaaga aagaaaattt taggagaaga ttttcatgtt actataaaac
      541 ctatatataa cagagaagat acattggcat ttaccttttc tccggaccag aaaaaagtag
      601 agattggtga agaaattaaa attacagtta agtatcctac agaaaagtat gatgctttct
      661 gtgatgaatt agtagaactt atagctagat atcaagaaat ataaaaaaaa cagtccatat
      721 ggactgtttt ctattattaa gcagcatttt tacctataaa tttatgtaca attttttcac
      781 atggtggcat aaaaaattgt atagctggac ctgtgaaaag tactgctatt attgttccaa
      841 gacctacaac accagctagt aaataaccaa ctactaggaa acttaaatct aaaccgatac
      901 gtatccaacg gtattggaaa ttagttttat ctcttattat aagaggaact aaatcgtttg
      961 gtgcaactcc taattcagta ctaactaaga atgaaaatcc tatagctatt ataatacagc
     1021 ttacagctac aattacgcat tttgtaaata agttaaagtt actaagtgaa gtatctaaac
     1081 caaatctatg taatattccc atgattgcat ctatagtagg ccctactact aaaacaagta
     1141 caaaagttcc aactttaagt tgttttctat ctgcaatgaa gattgcaata actaaagtaa
     1201 caagtattat catgttagca gttccagttg aaacgcttag tgcttttgaa agcccttgag
     1261 taaatacagt aaatggatct gatccagctc cacttaataa gaatattaca acacctaatt
     1321 gtataattgc cattcctaat aagaaaaata taagcttagg aataaatttt tcttttttca
     1381 tttttaatac actccttgtt tttgagtttt gtgaatcgta tttttgaatg tgaatttaaa
     1441 tatttaatgt gtatttaaaa acttaaaaga aaaccaaaca aaatgattat aaccattgtt
     1501 aatctatatg tcaaataaat ttaataactt aataaaaaat tcataactaa agaaatttaa
     1561 tatactaaaa ttcactttat tagaataaat aagaaaattg aaatgtactt gattttattt
     1621 tataattatt acgttagatg ggagaggtgg aaaatatgaa ggatactaag actaaagaac
     1681 atattgctag gatagcaaaa gcttcaacat attttatttt tagaaatgga cctgtaaata
     1741 agcttcataa agaaaataag gtttctgatg aagaacttaa agaaatgcaa gagtatatgc
     1801 aaaatcactt agcatatctt tatgaagttt tacttgaaga gggtaattta aaaaaatatg
     1861 aacttgtaat gaatactatg aatcaatttt atgtaaatga tgatacagaa gtagtattag
     1921 ctgatgaggg ctttgattct ttatatgatc aattattccc taaaagtagc aatataattc
     1981 ttaaataaaa aatgactatg agaaattctc atagtcattt ttttaattac aactaacctt
     2041 tggaatgaaa tcttcttcag cctcttcaat tttaatgtta gtattgtttc ttttctttaa
     2101 tattgattct actattatag ctatagcccc atctccagtt acattagttg ctgttccaaa
     2161 actatcttga gcagcatgaa gtgctatcat taatgatttt tcaatattac caaatccaag
     2221 cataccttca agtaatccta aagcagccat aactccgcca cctggtacac ctggtgctgc
     2281 taccatagtt actcctaaca taattataaa tggaaccata atagagaaag ttggaacttg
     2341 tcctttcata tacattacag acattgatgc aagaactaga gtaatagtat cacctgctaa
     2401 atttattgtt gctcctaaag gaataacaaa gtctgctaca tcttggtcaa ctttattttc
     2461 cttagtacag tttaaagtaa ctgggattgt agctgctgat gattgagttc ccaaagctgt
     2521 catatatgca ggaatcatat ttttaagtaa ttttaatgga ttttctttct ttaaagttcc
     2581 agctaaagca tattgaacca ctaaataaga tccttgtatt gaaaataaaa ctaagtacac
     2641 aagtccaaaa gactttaaag tagtaaaaat ctcaccatta taacttaact ttgaaaatat
     2701 tgaaagtatg taaataggta ctaaaggtat taacacattt tttatagtta atgaaataac
     2761 gtgattaaat tcttctgata cttttaataa gtaactattt tttattcttg atattcctat
     2821 tcctaataaa aatgcaaaaa ctaaagctga cataacacca aacattggag gaatatcaat
     2881 tgtaaaataa ggatcaacat ttactgatcc agtgcttaga gaagcactta ctataaattt
     2941 aggaagtact gcgcgtccta taaagaaagt aagtattcct attaaaaatg ttgaagcata
     3001 tgaaattaaa gtagttatga aaagtccttt tcctgattta tttcctaagg atgctatacc
     3061 aggaactata aacccaacta ttataagtgg aattaagaaa cttagtaaat tcccaaatat
     3121 gctattaaag gtcattaata ttctaatagg tatatctaaa ttaatatagg aacatcctag
     3181 cccaataagt attcctaaaa taattgcaac aaatattctt tttattaagg ataaattttt
     3241 cataatagcc cccttttgta ctcttattaa atacatatgt ttttcgtatg aatacatatt
     3301 actatagtta agatttaaag tcaacaataa aatctaaact attttataaa ttattaagta
     3361 atttttgttt gtaattttta tttatcaata aaaaaatatt gacattttac aaatttaatc
     3421 tcataattat taatataaag tgaataactg agatagaggc gcgatgatta atagtatctt
     3481 tgcagaggta agcacattga agcaaagtga aaggatgaat cgccgaaacc attagaagag
     3541 gctttaattc tattaggttg gggttacata gaatatatgt aacactgtca caaattattt
     3601 tgtggtgtgc tatcatgaaa gaagagtatt gtatataact ttacgaaccc ttaagcatga
     3661 aacacgctta agggtttctt ttgttagcga aaacttagtt atcacttatg gcttaattat
     3721 taaaaccata aggaggacta aaaaatgagt caagaacaaa atactaatct aaaaagaggc
     3781 ttgaaatcaa gacatcttag tatgattgcc atgggtggcg ccataggaac tggtattttc
     3841 ttagcaatgg gagatacact tcatcaagcc ggtgttggag gagcttttgt agcctacgga
     3901 atcataggag taatggtata tttcttatgt acaagcttag gggagatggc aacatatatg
     3961 ccagaaacag gttcctttgg agcttatgca acaaaatttg ttgatcctgc tttaggattt
     4021 gctctaggat ggaattactg gtataactgg gctataacaa tagcggcaga aatggttgca
     4081 ggttctttat taatgaagtt ttggtttcct aatgttccag caatagtttg gagcgcatta
     4141 ttcttagtaa ttatattaac tcttaattta ttatctgcca aagcttatgg agaatcagaa
     4201 ttttggtttg cggctgtaaa agttgctact gttataatat tcttaatcgt aggagttctt
     4261 atgatttttg gaatattagg tggagataat gttggattca aaaacttcta tgaaaatggg
     4321 ggaccattta ttggaggagc aaaatcaatt ttcatgatct tcttaatagc tggtttctca
     4381 ttccaaggaa ctgaattagt aggggttgct gctggagaaa gtgaagatcc tgaaaaaaat
     4441 gtacctaagg ctgttaatac aatattttgg agaatcttat tattttattt aggaacaatt
     4501 ttagtaatag gtgctttagt gccaaatgct caagcaggag tagaaacaag tgcatttaca
     4561 atagtatttg aaaaattagg attagctgga gccgcttcta ttatgaatgc cataatacta
     4621 acatctgtct tatcagctgg taactcaggt atgtatgcat caagtagaat gctttatgct
     4681 atggctaaaa atggaatggc acctaagatt tttgaaaaaa ctaattctag aggagtacca
     4741 gttaatgcta ttataataac aacaataatt gcaagtttat gtttcttaac aggtatttat
     4801 gccgaaaata cagtttatgt atggcttgta gcagcatctg gattatcagg atttatagct
     4861 tggcttggaa tcgcaatatg tcattataga tttagaaaag cttatgtagc tcaaggtaaa
     4921 gatatgaata gattagttta taaagctaaa ttattcccac tagggcctat aatagcttta
     4981 atattatgtg gaataatagt tttaggtcaa ggatatgcat acttagatgt tagaaatgga
     5041 gtagactggg gtggattaat ttcatcttat attggattac ctctattctt tggattattt
     5101 gcatattata aaattaaata taaaactaaa ataatagact taaaagatgt tgacttaagt
     5161 gtagagcata tagatactga tgagagtata gaagatgaag tattttctta ctaaaaaaag
     5221 aggtggtttt ttcacctctt ttttatttta aaattaataa atttgtttta ttacataatt
     5281 ttgatctgtt aaagcttttt ttatttcctc tatgtgagag tgtccatttg tctctaaagt
     5341 tacttctata accatatttt taagagcatc ccttgcttta aaatgatcat gttcaagttt
     5401 tactatatta gcattagtat cagcaagaac tttagcaatc ttcaatattt gtcctggttt
     5461 atgaggaact tctaaactaa atccaaaaag tcttccttat ttttatcttt acaatttaat
     5521 ttagcagaag ctgctaatga gactgctcct gaagcttctg ctaaaagttt atgtttttca
     5581 cttaatgtaa agaatgcttc aacaatttca tgatctgata cagttattat atcatcaaca
     5641 tattctttta taaatttaaa tgttaaatca ccaggtcttt ttactgcaat tccatcagca
     5701 atagtatcaa cagcgtctaa agcaattatt tctcctgcct taaaactttt aaccattgca
     5761 tttgcacctt cagcctgtac tccaattact ttaacattag ggtttaaagc cttagcagct
     5821 agggtaactc cacttattaa gccaccgcca cctatagggc agagaataat atctacatca
     5881 tttaaatctt caaatatttc taaggctact gtaccttgtc cacaaattac atcaatatca
     5941 ttaaatggat gtataaataa ggctccttgt tcttgttcaa ttcttttagc ctcattaaaa
     6001 gcatcatcat aaacataacc tgataaaaca acattccctc ctaaatcctt tgttgattgg
     6061 atttttagta gaggagtagt ctttggcatt actatagttg atttaacatt agcttgtgat
     6121 gctgcaaaag ctacgccttg agcatgattt ccggcagaag aacaaacaac accttttgat
     6181 ttttcttcat ctgataaaga tcttatttta tttaaagcac ctgttaattg taaattttca
     6241 catttcatat acacttggtt tctagatttc tttgaaaaga cctcgctata tattaaaggt
     6301 gtttttatga ctacatcttc tatatttttt ttggctttaa taattttttc taaatacaaa
     6361 ataatcaccc ctcattctca ttatgtaaac cttttacaat ttaattataa caaataaatt
     6421 tgattttcca caaggaatat tttaaaaaaa attatgcaaa ataataatga aagaaggtga
     6481 taaatatgtc agattctata gttaaagtaa aaaagaatag taatggagaa attacagatt
     6541 acgttttaag aagtggaaag actgtttcta aagctgaagg tgtaaagctt gcagaagagg
     6601 gaatgataga tggagtaata atttctcatt caaagaaagg ggaagcttat ttaagaagta
     6661 ttcctgatgg aaaagaaagt aacaacttag acgaattatc ataattaaaa aaattgcgat
     6721 atgtttatat tataatgaag atagcttaaa tttagctatc ttcattattt tgcattctaa
     6781 tatctaaata cgatattaaa atagggtttt taatttatat aggtaattaa atttaaaaa
//
LOCUS       DAEVGI010000135         6793 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEVGI010000135 DAEVGI010000000
VERSION     DAEVGI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.135"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(25..1830)
                     /gene="ftsH"
                     /locus_tag="K8M77_002090"
     CDS             complement(25..1830)
                     /gene="ftsH"
                     /locus_tag="K8M77_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBI7086556.1"
                     /translation="MKKLQSMITYIAIFAVVLMFAFAFYRNGTQGKVISYTEFKEAYV
                     GNKIETMTIKEDKMSVDGVFKDGKRFTSYVSNNMLDNLLQETKGVETVIKYTPPNNMG
                     IWISFLPTILIIGVIFFGLFMFTQQAQNSGGNRGVMNFGKSKAKMANLDGKKVTFKDV
                     AGADEEKGELEEIVDFLKQPKRYIEMGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGHDE
                     REQTLNQLLVEMDGFGVNEGIIMIAATNRPDILDPALLRPGRFDRRILVGAPDVKGRE
                     EVLKVHTRNKHLSEDVDLKVLAKMTPGFSGADLENLTNEAALLAVRGGKSSIDMSDIE
                     EAITRVIAGPEKKSRVVSEYDRRITAVHESGHAVVSNVLEYADPVHEISIIQRGMAAG
                     YTMNLPEEDRTHTSKKQLKDKMVELLGGRVAEKLVIGDISAGAKNDIDRASHIARSMV
                     MEYGMSDVIGPISFGNSDGGEVFLGRDIGKSSNISEETSAKIDEEIKKLIDEAYNRAE
                     SILRENISKLNAVTDVLLQKEKIDGDEFREIFKNS"
     gene            complement(1906..2457)
                     /gene="hpt"
                     /locus_tag="K8M77_002091"
     CDS             complement(1906..2457)
                     /gene="hpt"
                     /locus_tag="K8M77_002091"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBI7086557.1"
                     /translation="MIRDIKDDVKKVLYSEEQLAKRVKEIAEEISKDYAGKDLLVVGI
                     LKGSVLFTSDLIKNITIPCEIDFMAVSSYGNSAQTSGVVRILKDLDSDIENKHVLIVE
                     DIVDTGTTLSYLLEYLKARKAASIEIVALLDKEARRTSNISAKYKGFDVPDEFIVGYG
                     IDYAEKYRNLPFIGALKEEIYNK"
     gene            complement(2460..3869)
                     /gene="tilS"
                     /locus_tag="K8M77_002092"
     CDS             complement(2460..3869)
                     /gene="tilS"
                     /locus_tag="K8M77_002092"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBI7086558.1"
                     /translation="MKKKVIDFIKENSMIKSGDKVLVALSGGPDSVCLLHILSELKEL
                     LHIEVYAAHVNHCLRGESALKDEAYVEELCKNLNIKCFVKRVDINKISEEQNISTEMA
                     GREERYKFFEELKNEYSLDKIAIAHNANDQAETLIMRALRGTGIEGLVGIKPVRDGIF
                     IRPILILRRKEIEEYCEINNLTPRIDETNLEEIYSRNKIRLKAIPFIEENFNPDIVNT
                     LNRLAYSCSKDVEFIQEEVEKRFTKLCIKENNSILIKEEAFNAKEALLTRIIKKALFE
                     VSSKHNNFELKHIQDIIALKDKGTGKQINITNGVIALNEYGDIRIKLVDSKKVKDNKV
                     LNLENIKDELDKNQKVVIEDDILGNYELIVEDLKKGEKFSKDRFIKSFDYDKISNIDI
                     RFRQNGDKIIPLGMKSSKKLKDIFINNKIPKEERDFIPLVLFNNEIAWIVGSNVSETF
                     KVTNKTKKVIKITFKGKEN"
     gene            complement(4084..6474)
                     /gene="spoIIE"
                     /locus_tag="K8M77_002093"
     CDS             complement(4084..6474)
                     /gene="spoIIE"
                     /locus_tag="K8M77_002093"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBI7086559.1"
                     /translation="MQYGVKFDNYKRIKGSENVKNNMKIEASSIGLIMAVFVGFLISR
                     VYLDMTFGVVQVLAPFGLAYLIAITNYERKYILSSSLGVIVGYITLFNKVSNFSAYII
                     ISTIVTIISMSKLNKKARNISNFIMVFIGLFIYGVLVGSSDIILHLIGAISVTALVFP
                     IYYVVSYTLKCIEEINTQHFFSIDEIVSIELFICLLIVGIGTLSINDISFRNIAAILF
                     IIALAFISDTNMGAGAGITMGIILGFATGNLMESIAIYGACGLVAGIFRESGKLFTAL
                     SFNIIFIIVTLYSGVFNNISFVETLVGTVIFLLIPKKIYNKISLEINKDKKVGHFSEV
                     RFSEIKDELTERLKDFTEVLSVMGKSLNNLVSNDKLAIKNKGNALVENLSDRTCSDCD
                     MRYICWKRELHQTYNAFSDLIRNYENNLGDFPHELEKKCIKKYALVKNLEDIMNIYMV
                     NETLKSRLGEGRKILSNHINNMSVTISEIVDEFGNELHLCTDVEKSIKKSLLKYGVNF
                     GSLICYNDKNGRIKIKMQMENCMGSQTCIKTVLPIISETIGKNMSIGSEGCNINRKNN
                     MCEIVVEEAPKYHINSHVAVATKEGEKFTGDSYSYGRTKDGNYITVISDGMGSGPEAG
                     LESKVSVEIIEKFMDVGFDEKIAIDAVNAIMSIKFSEDEKFSTLDMSKIDLYTGNAKF
                     MKVGAIESFIKRGNKVEVINSNTLPFGVLEEPDVDTVEKQVSNGDVIVSISDGILDVK
                     NDGSFDTTWLIEFLKNTKYRQPKDLSIAILEKAKELSGGKAKDDMTVVVSKVFAIN"
BASE COUNT         2161 a         1283 c          627 g         2722 t
ORIGIN      
        1 gaggtactta agtacctctt ttaactatga gtttttaaat atttctctaa actcatcacc
       61 atcaattttt tctttttgaa gtaacacatc agttactgca tttaatttac ttatattttc
      121 tcttaatata gattctgctc tattataagc ttcatcaatt aatttcttga tttcttcatc
      181 tattttagcg ctagtttctt cacttatgtt actactcttt ccaatgtctc tacctaagaa
      241 tacttcgcca ccatcgctat taccaaatga tataggtcca ataacatcac tcattccata
      301 ttccataacc atacttctag caatgtgact agctctatct atatcatttt tagcaccggc
      361 acttatatct ccaataacta atttctcagc cactcttcca cctaaaagtt caaccatctt
      421 atctttaagt tgtttcttag atgtgtgagt tctatcttcc tctggtaaat tcattgtgta
      481 tcctgcagcc attcctcttt gaattatact tatttcatga actggatctg catactctag
      541 aacattactt acaacagcgt gtccagattc atgaactgca gtgattcttc tatcatattc
      601 actaacaact ctactcttct tctctggccc agctatcact cttgttatag cctcttcaat
      661 gtctgacata tctatgctac ttttaccacc tctaaccgct aataatgcag cttcgttagt
      721 taagttttca agatctgcac cactaaatcc tggtgtcatt ttagctaaaa cttttaaatc
      781 tacatcttct gatagatgtt tatttcttgt atgaactttt agtacttctt ctctaccctt
      841 aacatcaggt gcacctacta aaattcttct atcaaatctt cctggtctta gtaatgctgg
      901 atctaagata tctggtctat ttgtagcagc tatcataatt attccttcat taactccaaa
      961 accgtccatt tcaactagta attggtttaa tgtttgttct ctctcatcat gaccgccacc
     1021 aagtccagca cctctttgtc taccaacagc atcgatttcg tctatgaata taatacatgg
     1081 agcatttttc ttagcttgct caaataaatc tcttactctt gaagcaccaa ctccaacaaa
     1141 catttcaacg aaatctgaac ctgatatact aaagaaagga acaccagctt ctcctgctat
     1201 agcttttgca agaagtgtct tacctgttcc tggaggccct actaaaagaa ctcctttagg
     1261 tattcttgct cccatttcta tatatctctt aggttgtttt aagaaatcaa caatttcttc
     1321 taattcacct ttttcttcat cagctccagc gacatctttg aacgtaactt tttttccgtc
     1381 taaattagcc atcttagctt tacttttacc gaaattcatt acccctctat ttccaccact
     1441 gttttgagct tgctgtgtga acataaataa accaaagaat atcacaccta ttataagtat
     1501 tgttggaagg aaactaatcc aaatacccat attattaggc ggagtatact ttattacagt
     1561 ttctacccct ttagtctctt gcaaaagatt gtctaacata ttatttgaaa catatgaagt
     1621 aaacctctta ccatctttaa aaaccccatc aactgacatt ttatcttctt ttatggtcat
     1681 tgtttcaatt ttatttccta catatgcttc tttaaattca gtatatgata ttacttttcc
     1741 ttgagttcca tttctataaa aagcaaatgc aaacataagc actacagcaa aaatagctat
     1801 ataagtaatc atactttgta attttttcat ctctatgccc cccctctctg accatcattt
     1861 ttaatgaatt ttaacatacg aaatagtatt ttacaatatt ttgaattact tattgtaaat
     1921 ttcttctttt aaagcaccga tgaatggtaa gtttctatat ttttcagcat agtctattcc
     1981 atatccaact atgaattcat ctggtacatc aaatcctttg tattttgctg aaatatttga
     2041 agttcttcta gcttctttat ctaatagagc aacgatttct atacttgctg cctttctagc
     2101 ttttaaatat tctaaaagat agcttaaagt tgttcctgta tcaactatat cttcaactat
     2161 aagaacatgc ttattttcta tatcactatc taagtctttt aatattctaa caactcctga
     2221 agtttgagct gagttgccat aacttgaaac agccataaaa tctatttcac atggtattgt
     2281 tatattttta ataaggtctg aagtaaataa tactgaacct tttaatatac caacaactaa
     2341 taaatctttt ccagcataat ctttactaat ttcttcagct atctctttta cacgtttagc
     2401 taattgttct tctgaatata aaactttttt aacatcatct tttatatctc ttatcataat
     2461 taattttcct ttcctttaaa agtaatttta attacctttt tagttttatt tgtgacttta
     2521 aatgtttcac taacattaga tcctacaatc catgctattt cattattaaa tagcactaag
     2581 ggtataaaat ctctttcttc tttaggtatt ttattattta taaatatatc ttttagcttt
     2641 ttactactct tcattcctaa aggaattatt ttatctccat tttgtctaaa tctaatatct
     2701 atattggaaa ttttatcata atcaaaagac tttataaatc tatcttttga aaatttttca
     2761 ccttttttta aatcttctac tattaattca taatttccta aaatatcatc ttctattaca
     2821 actttttgat ttttatctag ttcatcttta atattttcta aatttaaaac tttattatct
     2881 ttaacctttt tgctatctac taattttatt cttatatctc catattcatt taaagcaatt
     2941 accccgttag ttatgttaat ttgctttcct gtccctttat cttttaaggc aattatatcc
     3001 tgtatatgtt ttaattcaaa attattatgt ttagaactca cttcaaataa tgcctttttt
     3061 attattcttg ttaatagagc ttcctttgca ttaaaggctt cttctttaat aagaatgctg
     3121 ttattttctt ttatacaaag tttagtaaat ctcttttcta cttcttcttg tataaactct
     3181 acatccttac tacagctata agctaatcta tttaaagtat taactatatc tggattaaaa
     3241 ttctcttcta taaatggtat tgccttaagc cttattttat ttctagaata tatttcttct
     3301 aaattagttt catctattct aggagttaaa ttatttattt cacagtattc ttctatttct
     3361 tttcttctta aaatcaatat aggtcttatg aaaataccat ctctaactgg tttaattccg
     3421 actaatcctt ctatgccagt tcctcttaaa gctctcataa taagagtttc tgcttgatca
     3481 ttagcattgt gtgctatagc tattttatct agtgaatatt cattttttag ttcttcaaag
     3541 aatttatatc tctcttctct tccagccatt tctgtagaaa tgttttgttc ttcacttatc
     3601 ttatttatat ctactctttt tacaaaacat tttatattta aatttttaca aagttcttcc
     3661 acataagctt catcttttaa agcactttct cctcttaaac aatgattcac atgggctgca
     3721 tatacttcta tgtgtaataa ttccttaagc tcacttagaa tatgtaataa acatactgag
     3781 tctggaccac ctgataaagc taccaaaacc ttatctccac ttttaatcat ggagttttcc
     3841 tttataaaat ctataacctt tttcttcatt gtaactcact ccataaatat tatattttgc
     3901 cctaatatta taacacataa aaaatctctt atgttcttaa aaaagttcgt ataatcaatt
     3961 ttaaataata aatttaatta tttacttaaa aataaaagaa agtgtataat aacttttttt
     4021 aattaaaatc tatataaaaa ataaattata gattttattt agttcttata cactctcctt
     4081 ttcttaattt attgcaaaaa ccttagatac aaccactgtc atatcatcct tagcctttcc
     4141 tccacttaat tcctttgctt tttctaaaat agctattgat aaatccttag gctgtctata
     4201 cttagtattc tttaaaaatt caattaacca tgtagtatca aaacttccat catttttaac
     4261 atctaaaata ccatcactta tactaactat tacatcccca ttacttactt gcttttcaac
     4321 agtgtctaca tctggttctt ctaagacacc aaaaggaagt gtatttgaat ttataacctc
     4381 caccttattt cctctcttta taaaactttc tatagctcca actttcataa acttagcatt
     4441 tccagtatat aagtctatct tactcatatc taatgtagaa aacttttcat cttcactaaa
     4501 ctttatactc ataattgcat taactgcatc aatagctatt ttttcatcaa aaccaacatc
     4561 cataaatttt tctattattt ctactgagac tttactttca agtcctgctt cagggcctga
     4621 tcccatacca tctgatatta cagttatata attaccatcc tttgttctac catatgaata
     4681 tgaatctcca gtgaattttt ctccctcttt agtagcaact gctacatggg aattaatatg
     4741 atatttagga gcttcttcaa caacaatctc acacatatta ttttttctat ttatattaca
     4801 cccttcactt ccaatactca tattttttcc tatggtctcg cttattatag gaagaactgt
     4861 ttttatgcat gtttgagatc ccatacaatt ttccatttgc atcttaattt taattcttcc
     4921 atttttatcg ttataacata ttaagcttcc aaagttaacg ccatacttta aaagagattt
     4981 ttttatgctt ttctccacat cagtacatag atgtagttca tttccaaact cgtcaactat
     5041 ctcactaatt gtaactgaca tattatttat atgattagat aagatttttc ttccctctcc
     5101 taacctactc tttaaagttt catttaccat atagatattc attatgtcct ctaaattttt
     5161 aactagagca tatttcttta tgcacttttt ctcaagttca tgaggaaaat cacccaaatt
     5221 attttcataa tttcttatta aatctgaaaa agcattataa gtttgatgaa gttctctctt
     5281 ccaacatata tatctcatat cacaatcact acaagttcta tctgataaat tttcaaccaa
     5341 tgcattcccc ttatttttta tagctaattt atcattgcta actagattat ttaatgattt
     5401 acccattaca gataaaactt cagtaaaatc ttttaatctt tcagttaact catctttgat
     5461 ctcagaaaat ctaacttcac taaaatgacc aaccttttta tccttgttta tctccaaaga
     5521 aattttattg tatatttttt taggaattaa aagaaaaatt actgttccaa ctaaagtttc
     5581 tacaaatgaa atattattaa aaactccaga atataaagtt actattataa aaattatgtt
     5641 aaatgataag gctgtaaaaa gctttcctga ctctctaaat atgcctgcaa ctaagccaca
     5701 agctccataa atggctatgc tctccataag gtttcctgta gcaaatccta atattatacc
     5761 catagttata cctgctccag cacccatatt agtatctgaa ataaaggcta aagctataat
     5821 aaaaagtata gctgctatat ttctaaaact aatatcattt attgatagag ttccaattcc
     5881 aactattaat aaacatataa atagttctat acttacaatt tcatctattg agaaaaaatg
     5941 ttgagtattt atttcttcta tgcactttaa tgtatagctc acaacataat atataggaaa
     6001 aactaaggct gtcactgaaa tcgcacctat taagtgtaaa attatatctg aacttcctac
     6061 taaaactccg taaataaata atcctataaa taccattata aaatttgata tatttctagc
     6121 ctttttattt aatttactca tagaaattat ggtaactata gttgatatta ttatgtaagc
     6181 cgaaaagttg ctaaccttat taaacaaggt aatgtaccct actataactc ctaaacttga
     6241 tgataatata tattttcttt cataattcgt tattgcaatt aaatatgcta atccaaatgg
     6301 ggctaatact tgaactactc caaaagtcat atctaaatac actctgctta ttaaaaaacc
     6361 tacaaataca gccataataa gaccaatact tgaagcttct attttcatat tatttttcac
     6421 attttctgaa ccctttattc ttttgtaatt atcaaattta actccatatt gcatatttgt
     6481 cctcctaacc ttctatcttt atgaagatta taacaagaat tacaggaaaa ttttgtaaat
     6541 agataggttg aactcttatt tttttaagac ataataaaac aaaaatttta tgaaatttat
     6601 attattttac ataagtttta taatataatt gtcatttaat tggaaatgaa taaattgaaa
     6661 tgaatttaaa gaaatatatt atctttttta gactttacta aaaacatttt ttgttgaata
     6721 aagtatattt ttatctataa aaataattat atattaaata aaacaaaaaa actcatctta
     6781 tattaagatg agt
//
LOCUS       DAEVGI010000136         6681 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEVGI010000136 DAEVGI010000000
VERSION     DAEVGI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.136"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(113..304)
                     /locus_tag="K8M77_002094"
     CDS             complement(113..304)
                     /locus_tag="K8M77_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HBI7086560.1"
                     /translation="MITKDMTIGEVIRKMPTAAEVLMSFGMGCVGCPSAQAETLEEAA
                     IVHGINLDDLIEAINNIEY"
     gene            complement(414..893)
                     /locus_tag="K8M77_002095"
     CDS             complement(414..893)
                     /locus_tag="K8M77_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBI7086561.1"
                     /translation="MGYIMDLRKELPNPHRPLIMCSAGIIIIDKKGRVLLQKRTDNNK
                     WGLPGGSLELGESFEEAAIREAYEEVGLKVKSLSLFNVYSGKECYNKYPNGDEIYNAS
                     SIFISNDYEGEVVLDGEESADAVFFNKIDIPSLEEVNPPDRIVIKDIIEKLNIEKYI"
     gene            complement(1021..2307)
                     /locus_tag="K8M77_002096"
     CDS             complement(1021..2307)
                     /locus_tag="K8M77_002096"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBI7086562.1"
                     /translation="MSAFVVLGAQWGDEGKGKMTDYLAEEAEVVVRFQGGNNAGHTVE
                     VEDKQYKLHLIPSGILHDEKLNVIGNGVVVDPKALFTEIDYLEGLGVKVTPEKLIVSD
                     RAHLIMPYHITLDELKEKARGKNDIGTTCKGIGPCYTDKYERSGIRVCDLMHKDSFAE
                     KLKINIEMKNGYIKLLGGEELNFDEIYNEYMAFAERLRPYVKDTSVEIYNAIQADKNV
                     LFEGAQGMLLDIDYGTYPYVTSSNTTSCGVASGAGIGPNMVTNAVGIAKAYTTRVGKG
                     PFPTELENETGDWIREKGHEYGVTTGRSRRCGWLDLVILKTTTRVCGLTSLVVTKIDT
                     LAGLDKIQMCVGYELDGKVIDYFPASLEDLARCKPVYEEFEGWGEEVADARSYEELPE
                     NAKAYLRRIEEFTGTKVSIVGVGPKRNQTIRVREEL"
     gene            2787..3467
                     /gene="sdaAB"
                     /locus_tag="K8M77_002097"
     CDS             2787..3467
                     /gene="sdaAB"
                     /locus_tag="K8M77_002097"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBI7086563.1"
                     /translation="MNTYGVFDILGPIMIGPSSSHTAGAARLGKVARAICGKPMKKVT
                     FLLHGSFGKTYKGHGTDKALVAGILGMEPYDTKLRNSLEIAKEKNIEIEFKEVDLGDA
                     HPNTVKFIIEDIDGVFHEIVGSSIGGGSIKITEVNGSVVEFTGEYPTLIISHKDVPGA
                     VSKVTAIVYEHNINIAFMKVFRTERGKAARMVFEMDSPITKDIIDEIKKVESVEKLVV
                     INPVKEDI"
     gene            3468..4349
                     /gene="sdaAA"
                     /locus_tag="K8M77_002098"
     CDS             3468..4349
                     /gene="sdaAA"
                     /locus_tag="K8M77_002098"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBI7086564.1"
                     /translation="MIARSGAELLEICKENNFSLAEYAIQYEMESKNCTREDVIKGME
                     KVLQVMKEAANEGQEKEVYSVSGLIGGDAYKLKKYLEKGDTLTGNVMVGAMARALSCS
                     EVNASMGRIVACPTAGSCGILPAVILTVGERLNLSDEELIQGLLASSAVGMIIAQNAT
                     LAGAEGGCQAECGSAAAMGAAATVEMMGGTPEMALDAGAIVFKNILGLVCDPIAGLVE
                     VPCAKRNFAGAVSALTTADLVMAGINSKIPFDDTVEAMYRVGKSLPASLRETALGGLA
                     ITKTGLKLKEKVFGKDK"
     gene            4584..5756
                     /locus_tag="K8M77_002099"
     CDS             4584..5756
                     /locus_tag="K8M77_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBI7086565.1"
                     /translation="MNKIFGFTKEELSSLKATYTANEIYQQPELWKETLNIVENNKEA
                     INKFLEKNLNKDNVKIILTGAGTSAYVGDTIYLYLAKKLGKRVEAIATTDFVSNPDEY
                     IDENANTLLVSYARSGNSPESVGTYDLFEKKVKNLAHIVVTCNKEGELAKKSQEKKGN
                     LVLIMPEKSNDKSFAMTSSFTCMTLATLLLFDIANLEKNKKIVEELATKGQASLDNQW
                     KDAKAISDLGAERIVYVGSGALKGLCHEMALKNLELTSGKQVTVCESILGFRHGPKSI
                     INNNTLVIFLCSTNYYTALYDYDLIKEVYNDEGTQKVAVLSYFDRENLKEISHKYITA
                     NGSKLDDTFIAFNYIILGQMISFFNSLRLEISPDNPRPDGTVNRVVQGVIIHEYNN"
     gene            5776..6633
                     /locus_tag="K8M77_002100"
     CDS             5776..6633
                     /locus_tag="K8M77_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HBI7086566.1"
                     /translation="MNKIKSTKDMLLRAQKGGYAIPAFNIHNLETFQVVVETAAELNS
                     PVIIAGTPGTIDYACSDYIVAMAEVASQKYDIPIAIHLDHFEDPKLIKSHILTGFKSC
                     MIDASHLSFEDNVNMVKEVVDFAHRYDATVEAELGKLVGVEDDLVVDENEGAYTDPDS
                     ALEFVERTGVDSLAIAIGTAHGLYKGTPKLDFDRLVKIREKVYVPLVLHGASDVPDEL
                     VKKSIELGICKVNVATDLKIPFSDAVKDYFIANPQANDPRKYMTPGKEAMKKIVEHKI
                     LLCGSNGKA"
BASE COUNT         2293 a         1022 c         1000 g         2366 t
ORIGIN      
        1 gtatttaaat taatcttata tttaaactta ataattaact tttaaagtaa atattataaa
       61 aataagaagc acctattaaa aaagtacttc ttattttaaa cagagattaa aattaatatt
      121 ctatgttatt tatagcttct attaaatcat ctaagtttat tccatgaact attgcagcct
      181 cttctaaagt ttcagcttga gctgatgggc atccaacaca tcccatacca aagctcatta
      241 atacctcagc agctgttggc atttttctta ttacttctcc aattgtcatg tctttagtta
      301 tcataataaa ctacacctct cttaattaaa ttaaaagtta tatccttaat tatactccct
      361 ataaatttca aatacaagta tttcactctg aattataatc tttaaaataa aacttatata
      421 tacttttcta tatttaattt ttctattata tcttttatca caatcctatc tggtggatta
      481 acctcttcta atgaaggaat atctatctta ttaaaaaata cagcatctgc actttcttct
      541 ccatctaaga ctacttctcc ttcataatca tttgaaataa atatagaaga tgcattataa
      601 atttcatctc cattaggata tttattgtaa cactctttac cagaataaac attaaataag
      661 cttaaactct taaccttaag ccccacttct tcatatgcct ctctaatagc agcttcctca
      721 aaactttcgc caagctctaa acttcctcca ggaagtcccc atttattatt atcagttctt
      781 ttttgtaata atactcttcc ctttttatca attattatga ttccagctga acacattata
      841 agtggtctat gtggattagg aagttctttt cttaaatcca ttatatatcc cataaaattc
      901 tcctcttaaa ttacataatt ttattctaaa gtaatttaaa aatattatac actaaaataa
      961 aaaatagctt aagattaaat aatcttaagc tattttaaac taattattct ttactactta
     1021 ttaaagttct tctcttactc ttatagtttg atttctcttt ggtccaacac caactataga
     1081 aactttagtt cctgtgaatt cttctattct tcttaagtaa gctttagcgt tctctggtaa
     1141 ctcttcgtag cttcttgcat cagcaacttc ttcgccccat ccttcgaatt cttcatatac
     1201 tggtttacat ctagctaaat cttctaaact agctgggaag taatctataa cttttccatc
     1261 taattcatat ccaacacaca tttgtatttt atctaatcca gctaatgtat ctattttagt
     1321 tactactaat gaagttaatc cacaaactct tgtagtagtt tttaagataa caaggtctaa
     1381 ccaaccacat cttcttgatc ttcctgtagt tactccgtac tcatgacctt tttctcttat
     1441 ccaatcacca gtttcatttt caagctctgt tgggaatggg cctttaccaa ctcttgtagt
     1501 ataagcttta gctataccaa cagcattagt taccatgttt ggtcctatac ctgctccact
     1561 agcaactcca catgaagttg tgtttgatga tgtaacatat ggataagttc catagtctat
     1621 atcaagaagc attccttgag ccccttcaaa taatacattt ttatcagctt ggatagcatt
     1681 gtatatttca actgaagtat ctttaacata tggtcttaat ctttcagcaa atgccatata
     1741 ttcattatat atttcatcga agtttaactc ttctccacct aataatttga tgtatccatt
     1801 tttcatttca atgtttattt ttaatttttc agcaaaagaa tctttgtgca taaggtcaca
     1861 aactcttatt cctgatcttt catatttatc tgtataacaa ggacctattc ctttgcaagt
     1921 agttcctata tcattttttc ctctagcttt ttcttttaac tcatctaaag ttatatggta
     1981 tggcattata agatgtgctc tatcactaac tattaatttc tctggagtta cttttactcc
     2041 taagccttct aaataatcta tttctgtaaa tagtgcttta ggatctacta caacaccatt
     2101 tcctattacg tttaatttct cgtcatgtaa tattcctgaa ggtattaagt gtaacttgta
     2161 ttgtttgtct tcaacttcaa ctgtatgtcc agcgttattt cctccttgga atctaacaac
     2221 tacttctgcc tcttctgcta agtaatctgt catctttcct tttccttcgt ctccccattg
     2281 agctcctaat acaacaaatg ctgacatttt ttatttcctc cttataaaaa gaacattaac
     2341 attctttttt tataaaccat aacggttgat taactttctt caattagaca acgttcgtat
     2401 ttataggtat ttttgctata aatttcattg tcctatacca tattaacaga gtaaaactct
     2461 taggtcaagg caataaaaac gaatatttta acatattttc aattacaaag ttcactttgt
     2521 ttaattcaat taaataatat ttaatttaat aatatttctt cgtcaaaatt tttattaata
     2581 ttactctttt ccttatactt attcttatca ataaattgat tttttactaa atattttaat
     2641 tctaaaacta attaaattat taacattaaa tttagataaa atttcaattt tgatttttaa
     2701 aaatgttata ctatatttaa cgaaagaatt acttaattag acactaaaaa tcttatttta
     2761 ttaaatataa caaaggagga taaaaaatga atacatatgg tgttttcgat atccttggac
     2821 caattatgat tggaccatct agctctcata ctgcaggggc tgctagactt ggaaaagtag
     2881 ctagagcaat ctgtggtaaa ccaatgaaaa aagttacttt tcttttacac ggttcttttg
     2941 gcaaaactta taaaggtcat ggtacagata aagctttagt ggcaggtatc cttggaatgg
     3001 aaccttatga tacaaaactt agaaattctt tagaaatagc aaaagaaaaa aatattgaaa
     3061 tagagtttaa agaagttgat ttaggagatg cacatcctaa tacagttaaa tttataattg
     3121 aagatattga tggtgttttt catgaaatag taggatcatc aataggcggt ggaagtatta
     3181 aaatcactga ggtaaatggt agcgttgttg aatttactgg tgaataccca acactaataa
     3241 tctcacataa agatgttcct ggagctgtat ctaaagtaac agccatagtt tatgagcata
     3301 atattaatat agctttcatg aaagtcttta gaacagaaag aggaaaggct gcaagaatgg
     3361 tctttgaaat ggatagtcct attacaaagg acattattga tgaaattaag aaagttgaat
     3421 ctgttgaaaa actagttgtt ataaacccag taaaggagga tatttagatg attgcaagaa
     3481 gtggtgctga attattagaa atatgtaaag agaataattt ctccttagct gaatacgcta
     3541 ttcaatatga aatggaaagt aaaaactgta ctagagaaga tgtaataaaa ggtatggaaa
     3601 aagttcttca agttatgaaa gaggcagcta atgaagggca agaaaaagaa gtttattctg
     3661 tgagtggatt aattggcgga gatgcttata aattaaaaaa atacttagaa aaaggggata
     3721 ctttaactgg gaatgttatg gttggcgcta tggcaagagc cttatcttgt tcagaagtaa
     3781 atgcttccat gggaagaata gtggcatgtc caacagctgg ttcttgtgga atattacctg
     3841 ctgtaatatt aacagtagga gaaagattaa atctaagtga tgaagaactt attcaaggtt
     3901 tattggcatc ctctgctgta ggtatgataa ttgcgcaaaa tgcaacatta gccggtgccg
     3961 aaggtggatg ccaagctgaa tgtgggtctg ctgctgctat gggagctgct gccactgtag
     4021 aaatgatggg tggaacccct gaaatggctt tagatgctgg ggcaatagta tttaaaaata
     4081 tacttggtct agtttgtgat ccaatagctg gcttagtaga agtaccttgt gctaaaagaa
     4141 attttgcagg agcagttagt gccttaacaa ctgccgactt agttatggca ggcataaata
     4201 gcaagatacc ttttgatgat acagttgaag ctatgtatag agtaggaaaa tctttacctg
     4261 cttctctaag agaaactgcc ttaggtggtt tagcaattac taaaacagga cttaaattaa
     4321 aagaaaaagt ttttggtaaa gataaataat tcttttttaa tgagcatatg taattatgta
     4381 tgctcatttt atttttaaat aattagcatt caatattact atacgctttt taattttatg
     4441 ttgatattct tcaaagttac tagtaaatta ttgacattta ttacaataat attgtaatat
     4501 taacttataa taacatcatt atctttggtg tttttttatt tctaccaact ggtaatgaca
     4561 acattataat aggggtgaga tttatgaata aaatttttgg atttactaaa gaagaattat
     4621 ccagcttaaa agccacttat acagctaatg aaatatatca acaaccagaa ttatggaaag
     4681 agactttaaa catagtagaa aataataaag aagctataaa taaattctta gaaaaaaatt
     4741 taaataaaga caatgtaaaa ataatcttaa ctggagcagg aacatctgcc tatgttggag
     4801 ataccatata tctttatttg gctaaaaagt taggtaaaag agttgaagct atagcaacta
     4861 ctgacttcgt ttcaaatcca gatgaatata tagatgaaaa tgcaaatacc ctcttagttt
     4921 cttatgctcg ttctggtaat agcccagaaa gtgttggaac ttatgattta tttgaaaaga
     4981 aagttaaaaa tctagcacac atagttgtta cttgtaataa agagggtgag cttgctaaga
     5041 agtctcaaga aaaaaaagga aacttagttt taataatgcc agaaaaatca aatgataaga
     5101 gttttgctat gacaagctcc tttacttgta tgacattagc tactctactt ttatttgata
     5161 tagctaatct agaaaaaaat aaaaaaatcg ttgaagaatt agctactaaa ggacaagctt
     5221 ctcttgataa tcaatggaaa gatgcaaaag caatttcaga tttaggagca gaaagaattg
     5281 tttatgtggg ttctggcgct ttaaagggtc tttgccatga aatggctctt aaaaatttag
     5341 aattaacttc tggtaaacaa gttactgttt gtgaatcaat cttgggcttt agacatggtc
     5401 ctaagtcaat aataaataat aatactttag ttatattctt atgctctaca aattattata
     5461 cagctcttta tgattatgac ttaataaaag aagtttataa tgatgaaggt actcaaaagg
     5521 ttgctgtttt aagttatttt gacagagaaa atcttaagga aatttctcat aaatatataa
     5581 cagctaatgg atctaaacta gatgatacat ttatagcatt taattatata atattgggtc
     5641 aaatgatttc tttctttaac tctttaagac ttgaaatttc tcctgataat ccaagacctg
     5701 atggaacagt taatagagtt gttcaagggg ttataattca cgaatataat aattaattta
     5761 tgagaggtgt tttttatgaa taaaattaaa tctactaagg atatgctttt aagagctcaa
     5821 aaaggaggat atgctattcc tgcttttaac atacacaatc tagaaacttt tcaagtagtt
     5881 gttgagactg cagctgagct taactctcct gttataatcg caggtactcc tggaactatt
     5941 gattatgctt gttcagatta tatagtagct atggctgaag tagcctctca aaaatatgat
     6001 attccaatag caattcacct tgaccacttt gaagatccaa agcttataaa aagtcatata
     6061 ctaactggat ttaaatcttg tatgatagat gcttcccatc tttcctttga agacaatgta
     6121 aacatggtaa aagaagttgt agactttgcc catagatatg acgctactgt tgaagctgaa
     6181 cttggtaaac ttgttggagt tgaagatgac ttagtggtag atgaaaatga aggagcatat
     6241 acagaccctg attcagcttt agaatttgtt gaaagaacag gtgtagattc actagctata
     6301 gcaattggaa ctgctcatgg attatataaa ggaactccta aattagactt tgatagatta
     6361 gttaaaatta gagaaaaagt atatgtacct ttagttttac atggtgcatc tgatgttcca
     6421 gatgaattag ttaaaaaatc tatagaactt ggaatatgca aggttaatgt tgctacagac
     6481 ttaaagattc cattttcaga tgcagtaaaa gattatttca tagctaaccc acaggctaat
     6541 gacccaagaa aatatatgac tccaggtaaa gaggctatga agaaaatagt tgaacacaag
     6601 attcttcttt gtggtagtaa tggtaaagct taattattta attttatata taaaagggcg
     6661 tgtatcaaaa taactttttt a
//
LOCUS       DAEVGI010000137         6665 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEVGI010000137 DAEVGI010000000
VERSION     DAEVGI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.137"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(231..4628)
                     /locus_tag="K8M77_002101"
     CDS             complement(231..4628)
                     /locus_tag="K8M77_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HBI7086567.1"
                     /translation="MNKRKIAAIILATMITNLSATTIDVLAQELNTKDNSKVEVSHND
                     ESHQARVSKFDLYNSDKLDAYNQEFQISRSNIKSINNNGGKYNSSTIDKAIDGNLETH
                     WETGKPNDSNFTNEVVVTFNEITNIDRIVYSARRDSARGKGFAKEFEIYASLKDEGDD
                     FSLVSSGKYTESIRDLVEIKFNPTDFKRLKFKFKKAEQNWASAAEFMFYKPDEVYSSI
                     PKLFTDGTMSELSKEFNSLEKINAFKEKIKNHPLYNDFKEITDLAESLISNSKKEDVL
                     ELEMRGDSISEAQKRKVWNFQDWQITGLSARAGDKINVYVDVAEGDPTPTLLYKQSLT
                     QHGGSTNFQLKPGKNEITIPEINYESNGIPKDVIQGGDLFFTNYRSDNQKRAPKVRIE
                     GASKYPVFILGKSDENEVMKELEAYVEKIKAEPNTTPNIFAVSSKKSLEFVQATYALD
                     WYKKNNKTPKYTAEQWDQYIADAMEFWGFDNSKDVNSDFNFRIMPMVKNLSGGAFMNA
                     GNGVIGIRPGNQDAILAANKGWGVAHELGHNFDTGGRTIVEVTNNIMPLFFESKFKTR
                     TRITEQNIWENNTYPKVGLDDYSNNELYNKDDSTHLAQLAPLWQLYLYDNTFYGKFER
                     QFRERDFGNKNREDIYKSWVVAASDAMELDLTEFFARHGIRVDDKVKDDLAKYPKPDK
                     KIYYLNDLAMNYKGDGFTDNAKVSVSTSGSNGNIKLSFSVDDENKDNILGYEIRRDGK
                     YVGFTSNDMFVDTKSNLDEDGVYVVTPYDRKLNTLNPIEVNALQSTLSVNPVITLALG
                     EEFKPEEYIVAKDIKGNSLSESVKIKSSNVNTSKVGEYEVLYSLEDSKGNKYTKTSKV
                     NVVSRKEYMSDLTPKKSSNGWGTVRKDKSISGGVIGLTRDGDFVDYDKGLGLHSNAEY
                     VYDLEGKDYDYFESYVGVDKAMSPRPSSSVIFKVLVDGEEKFNSGVMRSTTPQKYVKV
                     DVKNAKELKLIVNDAGDGDSSDHASFGDAKLATLSSKPIIKGENLAYNMDEKVDLMKG
                     ITATDIEDGNITSKVQIKSSDFVEGKSGIFKVVYSVTDSDGLTSECSRTIAVTDKETQ
                     LSTLNWKSATIGSGAVRKDRAVSGNQIRLLNEDNSVETFAKGIGTHSYSEIVYNSEGY
                     DIFDTWVGIDRHVAEKKVSSVKFKVYVDGELKAETDVMRIDTPKKRLVVDVRNSKEIK
                     LVVDVADNGNTWDHADWADAKFRNLAEYDTSELNKALEEAKKLDLNNYTEETSEALKS
                     AISKGEEALTSKDKETINSALEELNKAMDSLVKVDLNAVINIPDKYLLKSIQNQLNKT
                     GDITLGDMYNLTTLTLSGVFDLSGLENAKNLETLNMDYNEVKDLRPLSKLKKLNTLNA
                     HEQFIAAGELKPSNGKVIGDSKVYNREGKNVVKTIRVVDKSGNTILEQEAKDEFTINT
                     KDLSPGLYGVHVLFEDKGFSGVMFYLFNV"
     regulatory      complement(4771..4854)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(5073..6461)
                     /locus_tag="K8M77_002102"
     CDS             complement(5073..6461)
                     /locus_tag="K8M77_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7086568.1"
                     /translation="MDISLNTLKNVEEVKIKKAVPAVLALMIFALVIDNSFKIISPDL
                     AKAFNISATAVSWQATLAGLVIGIGAIVYASLSDSVSVRTLLVLGIILICVGSLLGFI
                     FQKSFLIIVISRIIQSAGLGSAETLYVIFIAKYFKESEHKKYLGFSTSSYAISQVIGT
                     ITGGYISTYLSWQVLFLIPLLTLVIIPFILKYIPKEKNRAGNVDYIGFILVATVAATL
                     MIFVSGFQWEVLIGFIVALILFLIYISKNENAFISIKFFTNRNFISVLLVAFVIYSVQ
                     LAFIFMFPFLVESIYHYRLDQISLLLVPSYVLAATVGALSGKVSKKIGSKQCIQIAIL
                     GIIVSLIVGALFVKTSVLVFIFVMILFSSSFALMYAPMIDTLTRTIPKEKTGTAIGFY
                     NFCLNIATSIGITYVAILMENKYLGKNFTGLFSDLVAIQYSNVLLVLAVISLVALVLY
                     WTLIGRREKKAI"
BASE COUNT         2159 a         1192 c          691 g         2623 t
ORIGIN      
        1 ttaaaaatta taaataaaat ttaaaacact ttttaattta tattaagctt tagaattgta
       61 tcatattata ttaatcttaa aaatttttca taagcaaaaa aatatagagt agaatctctt
      121 tctatagact ctactctaaa tatatcttta ttaaaatcct tttaaaaagt tatttctttt
      181 atattataaa attaatttgt aagcaaaata actttatatt tttctattta ttatacatta
      241 aataaataga acattactcc actaaaacct ttatcttcaa ataatacatg aacaccatat
      301 aatcctggtg atagatcctt agtgtttatt gtaaattcat ctttagcctc ttgttctaaa
      361 attgtatttc cacttttatc aacaactcta atagttttaa ctacattttt tccttctcta
      421 ttatatactt ttgagtcacc tataacttta ccattagaag gctttaactc tcctgctgca
      481 ataaattgct catgtgcatt taatgtatta agcttcttta attttgataa aggtcttaag
      541 tctttaactt cattataatc catatttaaa gtttctaaat ttttagcatt ttctaagcct
      601 gataaatcaa aaactccgct taatgttaat gtagttaaat tatacatatc ccctaaagtt
      661 atatctccag ttttatttaa ttgattttga atagatttaa gcaaatattt atctggtata
      721 ttaataactg catttaaatc tacttttact aaagaatcca ttgccttatt taactcttca
      781 agggcagaat ttattgtctc tttatcctta gaagttaagg cttcttctcc ctttgatatt
      841 gcacttttta aagcttcaga agtttcttca gtgtaattat ttaagtctaa ttttttagcc
      901 tcttctaatg ccttatttaa ctctgatgta tcatactctg ctaaatttct aaacttagca
      961 tctgcccagt ctgcatgatc ccaagtgttt ccattatcag ctacatctac tactaattta
     1021 atctctttag aatttcttac atccacaact aaacgtttct ttggagtatc aattctcatt
     1081 acatctgttt cagcctttaa ttctccatca acatatactt taaacttaac actagaaact
     1141 tttttctctg caacatgtct atcaatacct acccaagtgt caaagatatc atacccttct
     1201 gaattataaa ctatttctga atatgagtga gttcctattc ctttagcaaa ggtttcaaca
     1261 gaattatctt catttaataa tcttatttga tttccactta ctgctctatc ttttcttaca
     1321 gcacctgaac ctatagttgc agatttccaa tttaaagtag ataactgagt ttctttatct
     1381 gtaactgcta tagttcttga acattcagaa gttagtccat cactatctgt tactgagtac
     1441 acaaccttaa atattcctga ctttccttca acaaagtctg aagattttat ttgtacttta
     1501 gaagttatat ttccatcttc aatatctgtt gcagttattc ctttcattaa atctactttt
     1561 tcatccatat tataagctaa attttcacct ttaattattg gttttgaaga taaagtagct
     1621 aatttagcat ctccaaagct tgcatgatct gaactatctc catctccagc atcatttact
     1681 attaatttta gctccttagc gttttttaca tcaaccttaa catatttttg aggtgttgta
     1741 cttctcatta ctccactatt aaacttttct tctccatcaa ctaaaacctt aaaaataact
     1801 gaagaggatg gtctaggtga cattgcttta tcaacaccta cataagattc aaagtagtca
     1861 taatcttttc cttctaaatc atatacatac tccgcatttg aatgaagacc taatccctta
     1921 tcgtagtcaa caaagtctcc atctcttgta agacctatta ctcctccact tatgctctta
     1981 tcttttctaa cagttcccca tccatttgaa gattttttag gagttaaatc actcatatac
     2041 tcttttctac ttaccacatt aaccttagaa gttttagtgt atttatttcc tttagaatct
     2101 tctaagctat ataagacttc atattctcca actttagaag tatttacatt gcttgattta
     2161 atttttacgg attcacttaa agaattacct tttatgtcct tagccacaat atattcttct
     2221 ggcttaaact cctcacctaa agctagtgta atcactgggt ttacagataa agttgattgc
     2281 aatgcattta cctctattgg atttaaggta tttaactttc tatcatatgg tgttactaca
     2341 tatacaccat cctcatctaa attagattta gtatcaacaa acatatcatt agaagtaaat
     2401 cctacatact ttccatctct gcgtatttca tatccaagta tattatcttt attttcatca
     2461 tctaccgaaa atgaaagttt tatattacca tttgaaccac ttgtgcttac agataccttt
     2521 gcattatctg taaatccatc acctttataa ttcattgcta aatcatttaa gtaatagatc
     2581 tttttatcag gctttggata cttagctaaa tcatctttaa ccttatcatc aacacgaata
     2641 ccatgtcttg caaagaactc agttaaatct aactccatag catctgacgc tgccacaacc
     2701 caagatttat atatatcttc tctattttta tttccaaaat ctctttctct aaactgtctt
     2761 tcaaactttc catagaaagt attatcatat aaatataatt gccataatgg tgctaactga
     2821 gctaaatgag tactgtcatc tttattatat aactcattat tagaataatc atctaagcca
     2881 actttagggt aggtattatt ttcccatatg ttttgttcag ttattcttgt tctagttttg
     2941 aatttagatt caaagaataa tggcataata ttattcgtta cttctactat ggttcttccc
     3001 cctgtatcaa aattatgtcc aagctcatga gcaacacccc atcctttatt agctgcaagt
     3061 attgcatcct gatttccagg tcttatacct ataacaccat ttccagcatt catgaatgct
     3121 ccaccactaa gatttttaac cataggcatt attctaaaat taaaatctga attaacatct
     3181 tttgagttat caaatcccca gaattccata gcatcagcta tatattgatc ccattgctcc
     3241 gctgtgtact ttggagtttt gttatttttc ttataccaat ctaatgcata agtagcttgt
     3301 acaaattcta atgatttctt agaacttact gcaaaaatat ttggagtagt attaggttct
     3361 gctttaattt tttcaacata ggcttctaac tctttcatta cttcattttc atctgatttt
     3421 cctagaataa atacaggata tttgcttgcc ccttctattc ttacctttgg tgctctcttt
     3481 tgattatcac ttctataatt agtgaagaat aaatctcccc cttgaataac atccttaggt
     3541 attccattag attcataatt tatttctggt atagttattt catttttacc aggctttaat
     3601 tgaaaatttg ttgatccacc atgttgagtt aaagattgtt tatataataa cgttggagta
     3661 ggatcacctt ctgctacatc cacatataca tttattttat ctccagctct agcagataat
     3721 cctgttattt gccaatcttg gaaattccat acttttctct tttgagcctc acttatagag
     3781 tctcctctca tttctaattc aagtacatcc tctttttttg aattagaaat taatgattca
     3841 gctaagtctg taatttcttt aaagtcatta tatagtggat gatttttaat tttttcttta
     3901 aaggcattta ttttttctaa actattaaat tctttactaa gctcactcat agtaccgtct
     3961 gtaaaaagct taggtattga agaataaact tcatctggtt tataaaacat aaattctgct
     4021 gctgaagccc agttttgttc agccttttta aatttaaact ttaatctttt aaagtcagtt
     4081 ggattaaact ttatttcaac taaatctcta attgattctg tatattttcc actgcttact
     4141 aagctaaaat catctccctc atctttaaga gaagcatata tttcaaattc ttttgcaaag
     4201 ccttttcccc ttgcagagtc tcttcttgca gaatatacaa ttctatctat attagttatt
     4261 tcattaaatg taactactac ttcatttgta aaatttgaat cattaggttt tcctgtttcc
     4321 caatgagttt ctaaattgcc atctatggct ttatcaatag tggaactatt atactttcca
     4381 ccattattat ttatggattt tatatttgat ctgctgattt gaaattcttg attataagcg
     4441 tctaatttat cactattata taaatcaaac ttacttactc ttgcttgatg tgattcatca
     4501 ttatgactaa cttctacctt agaattgtct ttagtgttta attcctgtgc taaaacatca
     4561 attgttgtag cagaaagatt tgtaatcatg gtagctaaaa ttatagctgc tatttttctc
     4621 ttattcattt tttctatcct cctgattgac tttcccctca gaagtgcatt tctacttata
     4681 atttaatata agcttgtaca aaataaattt ttaaatttta acctacaccc tcctccataa
     4741 ttaatataaa acaaaaagct taatgcactc tttggcaact ggctgacata gttataaacc
     4801 ttagtcccta tagctttgcg tcattagatt tctctaattt tgccgaattt taaagttata
     4861 atatcataat ttcttttaat tttatagtct ttataataaa tttaatataa atgagcattt
     4921 ttttaataat ttatacaaaa acttaataga tttataatat actaaacttt tataaacaaa
     4981 aaaacctata agatagtctt tttattgacc atcctacagg cttttaacac ttagtttttt
     5041 taactattat ttataagatt ctaaaatttt atttatattg cctttttctc tcttcttcca
     5101 attaaagtcc agtataatac aagtgctact aaagatataa ctgctaatac taataaaaca
     5161 ttactatatt gtatagctac taagtcactg aaaagtccag taaagttctt tcctaagtat
     5221 ttattttcca taagtattgc tacatatgtt attcctattg aagtagctat atttaaacag
     5281 aaattataaa aaccaatagc cgttcctgtt ttttccttag gtattgttct tgttaatgta
     5341 tcaatcatag gtgcatacat taaagcaaat gaacttgaga atagtatcat tacaaaaata
     5401 aatactaaaa ctgatgtttt tacaaataaa gctcctacaa taagacttac tattatacct
     5461 aatatagcta tttgaataca ttgcttactt cctattttct tagaaacctt tcctgataaa
     5521 gctcctacag tagcagctaa aacatatgaa ggaactaaaa gtaatgaaat ttggtctaat
     5581 ctataatgat atatagattc aactaagaat ggaaacataa atataaatgc aagttgtaca
     5641 ctatatataa caaaagctac aagtaatact gatataaagt ttctatttgt gaaaaattta
     5701 atgcttataa aagcattttc atttttactt atgtatataa ggaataatat aagtgcaact
     5761 ataaatccta ttaaaacttc ccattggaat cctgaaacaa agatcattaa tgttgctgca
     5821 actgttgcaa ctaatataaa tcctatataa tccacattac cagctctatt cttttcctta
     5881 ggaatatatt ttaatataaa aggtattatc actaaagtta ataatggaat taaaaatagt
     5941 acttgccaac ttaaatatgt tgaaatatat cctccagtaa ttgtaccaat tacctgtgaa
     6001 atagcgtaac tacttgtact aaatcctaag tattttttat gttcactctc tttaaaatac
     6061 tttgctataa atattacata taatgtctct gcacttccta atccagctga ctgaattatt
     6121 cttgaaataa caataattaa aaatgatttc tgaaaaataa atcctaatag tgaaccaaca
     6181 catattaata taattcctag taccaataaa gttctaacac ttactgaatc agataaagaa
     6241 gcatatacaa tagctccaat accaataact aatccagcta aagttgcttg ccaactaaca
     6301 gcagtagctg atatgttaaa agctttagcc aaatctggtg aaataatttt aaatgagttg
     6361 tcaataacta aagcaaaaat cattaaagcc aatacagcag gtaccgcttt ttttattttt
     6421 acttcttcaa cattttttaa agtattaagt gaaatgtcca ttttaaatcc tctcttttct
     6481 ttttttcatt tgtgcttctt aaataagagc ttgctaataa tcataataaa cttgtctact
     6541 ctttttactt catcctccct caataatcgt ttacaaatta tactaaaacc aacttattta
     6601 gcggtaagta aacttgtcta agttttactt attttttaga caagttaata ctatcatatc
     6661 tttat
//
LOCUS       DAEVGI010000138         6506 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEVGI010000138 DAEVGI010000000
VERSION     DAEVGI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.138"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            69..1307
                     /gene="uvsE"
                     /locus_tag="K8M77_002103"
     CDS             69..1307
                     /gene="uvsE"
                     /locus_tag="K8M77_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HBI7086569.1"
                     /translation="MKIGYACTPVTTNARTNRRILLKDFSKDKFLSITKQNLDDLQKI
                     LEWNIKNNIYLFRIGSDIIPLGSHEINNISWQKEFKDELETIGTFIKNNKIRVSMHPG
                     QYTVINTPKEDVLYKSIKDIEYHCEFLDSLNVDYKNKIILHIGGVYGDKKLAKENFLK
                     GFKKLSDSSKKRLVIENDERNFSLDDVLDISSKLNIPVIFDNLHNICYGDNSYSLKEI
                     YSLVIKTWNKELDGNMKVHYSEQDIFKKKGSHSPSISINSFLEYYEEVKEYSPDIMLE
                     VKDKDISAIKCINSLKEINKTLNSKAYREEIENYKLLLLQHDKDFQKKLNSFSKGLIE
                     FYNYLDKLLLSPKEIIGFKYSLELAFNILKDHISNRESLYFKKLINEKEYEKAKVYLT
                     KLVKKIEFPPKELSYYISQS"
     gene            1377..2000
                     /gene="ytaF"
                     /locus_tag="K8M77_002104"
     CDS             1377..2000
                     /gene="ytaF"
                     /locus_tag="K8M77_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBI7086570.1"
                     /translation="MEAEKVSFLSILLFVIAASLDILVVSLAYGLKDIKINFSSTLVI
                     ASISALGTFISMILGKFLVDLIPIKLGDIIGGLVLLLLGFYSIYSYFKEKKILTSHNS
                     ENDSSPTFILENPEVADKDKSGNIDFKESLALSLALALNNFGLGIGASISGLNIAFTT
                     ISTFIISLIFISLGFYLSKTIKSKNISKNSNLIAGIIIIILSLFIIF"
     gene            complement(2169..3338)
                     /locus_tag="K8M77_002105"
     CDS             complement(2169..3338)
                     /locus_tag="K8M77_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
                     /protein_id="HBI7086571.1"
                     /translation="MIVEPKFRGFICTTSHPIGCKKNVENQIEYVKENGKIEGAKRVL
                     VLGASTGYGLASAIVASEACDAEVLGVSFEREAKGKRTASAGWYNIESLKKFVEGEGK
                     KFISVNGDAFSNEVKSEVIDLIKENMGKVDLVIYSLAAPKRKDPVSGEVYSSCLKTVG
                     APFTSKTLDFHTGEIQDITINPATEEEIEGTRKVMGGEDWMLWIEALKEANVLENGVK
                     TIAYSYIGPEVTYPIYREGTIGRAKNDLEKTAGEITKVLKSLNGEGYISVNKALVTQA
                     SSAIPIVSLYISILYKVMKEKGTHEGCIEQIYRMFKELYEGNLNLDSENRIRIDDLEM
                     AEDVQKAIEEIWPQITSENVFELSDAEDFKKEFFKLFGFGLEGVDYSEDVDITTV"
     gene            complement(3576..5066)
                     /locus_tag="K8M77_002106"
     CDS             complement(3576..5066)
                     /locus_tag="K8M77_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI7086572.1"
                     /translation="MGNSNDGKKLMWYNLGLMAFVSVWGFGNVVNNFATQGLTVITSW
                     ILIIALYFVPYALMVGELGSTFRDSKGGVSSWISKTMGPTLAYLAGWTYWVVHVPYLA
                     QKPQAVLVSLGWAVFQDGSTIKGIDSKIIQLVCLVVFLFFVWIASRGVNSLGKIGTIA
                     GTAMFVMSILYIVLMLTAPAITGTSIASPNMTSIKTYIPKFDFAYFTTIAMLVFSVGG
                     AEKISPYVNNMKDSKKGFSKGMIALAIMVAVTALLGSVAMGMMFDANNVPDDLMLNGA
                     YYAFQKLGNYYGIGNSLLILYALANFAAQVSALVFSIDAPLKVLLSDTDARYVPIALT
                     KTNKNGAPINGYIMTSILVGILIIVPALGSGNFNALFTWLLKLNAVVMPMRYLWVFLA
                     YIMLRKAIKGKFKSEYKFVKNDKFAMLIGTWCFVFTAFACILGMFPTDVKAFSGEWIF
                     RVGMNIGTPLVLIGLGLILPKIAKRTNGQAYKDAVREATATKLELN"
     gene            complement(5263..6156)
                     /locus_tag="K8M77_002107"
     CDS             complement(5263..6156)
                     /locus_tag="K8M77_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBI7086573.1"
                     /translation="MKKYVVIDIGGTSIKHALMTESGDILEKGSMKTEGDNIDIFIEA
                     IGKVVDSYKEKNEVFGLACSLPGAVDVKTGFIGGGSAIPCIHGPNIRELLEKRCGLEV
                     SIENDANCAGLAEGWLGSAKGVENYACIVIGTGIGGCIVINGRILRGKHLHGGEFGYM
                     FTRDSEGLTDRIWSEVSSTNALVTRVSKLKGIEKSELDGKKVFEMAEDGDEEVNNEIE
                     SWYMDLARGIYNIQYIVDPEKIVIGGAISARHRFDKKINEKLALLKSDIATLDISVEK
                     CKFQNDSNLIGALYNFLFVRR"
BASE COUNT         2240 a         1120 c          723 g         2423 t
ORIGIN      
        1 atttttgttt tattttttta tttaaatgca aaacatataa ttaatatttt gcaaaggatt
       61 gattatatat gaaaatagga tatgcttgta ctccagtaac tacaaatgct agaacaaatc
      121 gcagaatatt actaaaggat ttttctaagg ataaattcct ttctattaca aagcaaaact
      181 tagatgattt acaaaaaata ttagaatgga atattaaaaa taatatttac ttatttagaa
      241 taggatcaga tattatcccc ttaggttccc atgaaataaa taatataagc tggcaaaaag
      301 aatttaaaga tgaattagaa accataggaa cttttattaa gaataataaa attagagttt
      361 ctatgcatcc tggtcaatat acagtaataa atacccctaa agaggatgtg ttatacaaat
      421 ctataaagga tattgagtat cactgtgagt ttttagactc cttaaatgta gattataaaa
      481 ataaaataat acttcatata ggaggggtat atggcgataa aaaattagca aaagaaaact
      541 ttttaaaggg atttaaaaag ctttcggatt cttctaaaaa gcgattagtc atagaaaatg
      601 atgagagaaa tttttctcta gatgatgttt tagatatttc tagtaaatta aatattcctg
      661 ttatatttga taatttacat aatatatgct atggggataa ttcttatagt ttaaaagaaa
      721 tttactcact tgttattaaa acatggaata aagagttaga tggaaatatg aaagtacatt
      781 atagtgagca ggacattttt aaaaagaaag ggtcccactc tccttcaatt tctataaata
      841 gttttttaga atattatgag gaggttaaag agtattctcc tgacataatg ttagaagtta
      901 aggataagga tatctctgca attaagtgca taaactcctt aaaagaaata aataaaacac
      961 taaactctaa agcttatagg gaagagatag aaaattataa attacttttg cttcaacatg
     1021 ataaagactt tcagaaaaag cttaactcct tttctaaagg tttaattgaa ttttataatt
     1081 atttagataa attattacta tctcctaagg agataatagg atttaaatac tctttagaac
     1141 tagcttttaa tattttaaaa gatcacataa gtaatagaga aagcctatat tttaaaaagc
     1201 tcattaatga aaaagaatat gaaaaagcta aagtttattt aacaaagtta gtaaagaaaa
     1261 tagaatttcc ccctaaggaa ttatcttatt atatttctca gtcttaagct tactctccct
     1321 ccaaaattgt cacaatttat atattaataa aatatattgg aattaacagt aaaattttgg
     1381 aggcagaaaa agtgagcttt ttaagtatac ttctttttgt aattgctgca agtttagata
     1441 tacttgttgt ttctttagca tatggtttaa aagacataaa aataaatttt tcaagtaccc
     1501 ttgtcatagc atccatatct gctttaggaa cttttatttc aatgatactt ggaaaatttt
     1561 tagtggattt aattcctatt aaattaggag atattatagg tggtttagtt cttcttttat
     1621 taggttttta ttctatatat tcttatttca aagagaaaaa aatcctaacc tcacataata
     1681 gtgagaatga ttcctctccc acctttatat tagaaaaccc agaagttgca gataaagata
     1741 agtctggtaa tattgatttt aaagaatccc tagccttatc cttggcccta gctttaaaca
     1801 actttggttt aggaataggt gctagcatct ctggactcaa tattgccttt acaacaatat
     1861 caacctttat tatttcattg atttttatct ccttaggctt ctatttatca aaaacaataa
     1921 aaagtaaaaa tatttcaaaa aatagtaact taattgcagg aataataatc atcatattat
     1981 cactatttat tattttttag atacaattaa gttttattca aaatataaaa atacattgcc
     2041 atacaaaaag caactataaa ttcttaaata aagtaacttt aaataaaata taaaaggcct
     2101 tactctagta acttaactaa agtatgacct tttcatattt ttaaaattaa tcaaataata
     2161 tttttatact aaactgttgt tatatcaaca tcctctgagt aatctactcc ctctaaacca
     2221 aatccaaata acttgaaaaa ttccttttta aaatcttctg catcacttaa ttcaaaaaca
     2281 ttctcactag ttatttgtgg ccatatttct tcaatagcct tttgtacatc ctcagccatc
     2341 tctaaatcat ctattctaat tctattttct gagtctaaat ttaagtttcc ttcgtaaagc
     2401 tctttaaaca ttctgtatat ttgctcaata cacccctcat gagttccttt ttctttcata
     2461 actttataaa gaattgaaat atataaagat actattggaa tagctgaact agcttgcgtt
     2521 actaaagctt tatttacaga tatatatcct tctccattta atgattttaa aaccttagtt
     2581 atctctcctg cagttttttc taaatcattt ttagctcttc ctatagttcc ttctctgtat
     2641 attggatagg taacttctgg tccaatatat gaataagcta ttgttttaac gccattttct
     2701 aagacattag cttcctttaa tgcttcaatc catagcatcc aatcttctcc acccattact
     2761 tttctagtac cttctatttc ctcctctgta gctggattaa tagtaatatc ctgtatttct
     2821 cctgtatgga aatctaaagt tttactagta aatggagctc ctacagtttt taaacaagaa
     2881 ctataaactt ctcctgaaac aggatccttt ctctttggag ctgctaagga atatataact
     2941 aaatctactt ttcccatatt ttcttttatt aaatctataa cttcactttt aacttcatta
     3001 gaaaaagcgt ctccatttac tgatatgaat tttttccctt caccttctac aaatttcttt
     3061 aatgattcta tattatacca tcctgctgaa gctgttcttt ttccttttgc ctctctctca
     3121 aatgaaacac ctaaaacttc cgcatcacag gcttctgaag ctacaatagc acttgctaat
     3181 ccatatccag tggaagcccc taatacaagt actcttttag ctccctctat ttttccattt
     3241 tcttttacgt attctatttg attctcaaca ttttttttac aacctatagg atgtgatgta
     3301 gtacatataa atcctctaaa ctttggttct actatcatta agatttttct cccttcttct
     3361 ttttcattta ttatttttaa aggctcatat tttctttagc cctttatcta ttctttttaa
     3421 tctttgatac tattttgttt gattatcaaa atatattgca attatcatat tatcatattt
     3481 catatatttt gtcattataa ttaatctaat aaaatcacca aatataatta tataattgta
     3541 taaaaaaaaa gacactctat agagtgcctt ttttactaat taagttctaa ctttgttgct
     3601 gtagcttctc ttactgcatc cttgtatgct tgtccgttag ttctcttagc tattttaggt
     3661 aatattaaac ctaatcctat taatactaaa ggtgtaccaa tattcattcc tactctgaaa
     3721 atccattctc ctgagaatgc tttaacatct gttgggaaca tacctaatat acaagcaaat
     3781 gctgtaaata caaaacacca agtacctatt aacattgcaa atttatcatt tttaacaaat
     3841 ttgtactctg atttgaactt tccttttata gcttttctta acattatgta agctaagaat
     3901 acccataaat atctcattgg cataacaaca gcatttaatt ttaataacca agtaaataaa
     3961 gcattgaagt ttccacttcc taaagctgga actattatta atattccaac taaaatagaa
     4021 gtcattatat atccgtttat tggagcccca ttcttattag tcttagttaa tgctattgga
     4081 acatatcttg catcagtatc tgataataaa acttttaatg gagcatctat tgagaatact
     4141 aatgctgaaa cttgagctgc aaagtttgct aatgcatata atattaataa agaatttcct
     4201 attccataat agttacctaa cttttggaat gcatagtaag caccatttaa cattaagtca
     4261 tcaggaacat tatttgcatc aaacatcatt cccattgcta ctgatccaag aagtgctgta
     4321 actgcaacca ttatagctaa ggctatcata ccttttgaga aacctttttt agaatctttc
     4381 atgttgttaa catatggaga tatcttttca gctcctccaa ctgagaatac taacatagct
     4441 atagtggtaa aataggcaaa atcaaatttt ggtatatagg tttttataga agtcatattt
     4501 ggacttgcta tagaagttcc tgtaatagca ggtgctgtta acataagtac tatataaaga
     4561 atagacataa caaacattgc tgttcctgct attgtaccta ttttccctaa tgaatttact
     4621 cctcttgagg caatccatac aaagaataag aatactacta aacatactaa ttgaataatt
     4681 ttagaatcta taccttttat agtgcttcca tcttggaata ctgcccatcc taatgaaaca
     4741 agtactgctt gtggtttttg cgctaaataa ggcacatgta ccacccaata agtccaacct
     4801 gctaagtaag ctaatgtagg tcccattgtt ttacttatcc acgaacttac gccaccttta
     4861 ctatctctaa aagttgagcc taattctcca accataagcg catatggtac gaagtataaa
     4921 gctattatta atatccatga agttattacg gttaaacctt gagttgcgaa gttatttact
     4981 acgttaccaa agccccaaac tgatacaaag gccattaaac caaggttata ccacataagt
     5041 ttttttccat cattactatt acccatgtac ttttctctcc taatcattaa attttagtgg
     5101 ctttcatata aattttttta aaatacttgt ctatttttaa aagaattcat agaaaatttg
     5161 tcacaaatta ttttaacgtt aaatattacc cattaatttt atcatcctat taaatttaaa
     5221 tcaatataat gtaattcctt tattaaaata aaataataag atttatcttc taacaaaaag
     5281 aaagttatat aatgctccta ttaaatttga atcattttga aatttgcatt tttcaacact
     5341 tatatctaaa gttgctatat cactttttaa taaagcaagt ttttcattta ttttcttatc
     5401 aaatctatgt ctagcactta tagctccacc aataacaatt ttttcagggt caactatata
     5461 ttgaatattg tatattcctc ttgcaagatc catataccat gattctattt cattattaac
     5521 ttcctcatct ccatcttcag ccatttcaaa aactttcttg ccatcaagtt ctgatttttc
     5581 tattcctttt aatttagata cccttgtaac taaagcatta gtagaggaaa cttcactcca
     5641 aattctatct gttaatcctt cactatctct agtaaacata taaccaaatt ctcccccatg
     5701 aagatgcttt cctcttaaaa ttcttccatt tataactatg catccaccta ttcctgtacc
     5761 aattacaata catgcataat tttctactcc tttagctgat ccaagccatc cttccgctaa
     5821 tccagcgcaa tttgcatcat tttctataga aacctctaaa ccacatcttt tttctaaaag
     5881 ctcccttata tttggaccat gtatgcaagg aattgcactt cctcccccta taaatcctgt
     5941 tttaacatct acagcacctg gcaaactaca tgctaatcca aaaacctcat ttttttcttt
     6001 atatgaatca acaacttttc ctatagcttc aataaaaata tctatattat ccccttcagt
     6061 tttcatgctt cctttttcta aaatatctcc actttcagtc atcaaagcat gttttatact
     6121 cgttcctcca atatcaataa caacatattt cttcatagaa aaatcctccc cactatacat
     6181 aatttataac ccaatgctta taaacattaa aattacattc tttataaaag taatgggctg
     6241 tatcaaaata taacaataaa atatttctaa gaaagctgct tttctaacat ttttcaaata
     6301 taggttatcc aatgaacaag taaatgctaa atcatatggt tctcttgaat ttattgcacc
     6361 cccatttttg agttgcaatc accgtaatca ataaatttca tatcaatagt agcgtttaca
     6421 ggtttattat atagcccatc taaacatttt catagtggag tttttttata tttatttgat
     6481 attctctatt tataaattta atgaaa
//
LOCUS       DAEVGI010000139         6411 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEVGI010000139 DAEVGI010000000
VERSION     DAEVGI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.139"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..2464
                     /gene="mprF"
                     /locus_tag="K8M77_002108"
     CDS             <1..2464
                     /gene="mprF"
                     /locus_tag="K8M77_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBI7086574.1"
                     /translation="TSVFKNFNSEYFFMYRNKLDFLNILIIAALGIISYIPLSFYDFI
                     LKRKVRIRLKNRKLYKYSWIASSIASLLGFGGATSLAFKQYFYGDYVDDKKKLLKEIG
                     KIVALNLTGLSIVCCTYMGIRISSWNNLGIIKYAIGIIALYAPGFIIYSAYKYSKTKD
                     KLEFFSTLGIIFISFLEWLTTIILIYETLRITGASISVLNFLPIYIESAVVGMISMIP
                     GGIGTFDLTFMTGLESLGIPIEQTLLGIILYRISYYIVPALIGVLLFVHDFGGKINKK
                     FNGLPYEIVSKVAYKIVVSLVFISGAIIVLSNIAPQYLLKIKLLKEILGKQVLGLSIG
                     MSVVLGFLIMLAALMLKYRAKSIYKASMVLFILGIILSLTKGINPYELVFLIIVAYLL
                     YLSKRMFYRDSFVVSCKNTLIDSGILIASFSIYFFILITFGTHLKYVGIVRKMPYKMA
                     YKFGFIAFALVTVIYVAIYFLNIRRKIPVKTFDQCSEYIEKIIEEYKGDSLTHLVFLK
                     DKYIYLNEDKDLFIQYEVYGDKLFVLGNPVGNNENLFREIEKFCEYTDNYGYTPVFYQ
                     VNEEMISYLHSNGYDFMKIGEEAKVDVKEFKVVGNKMKSLKTSRSKVTKEGYTFHMVE
                     PPFSREFLDSLKEISDEWLDGRKEKGFSVGFFDEDYLNKAPIAILRDREGEIKAFANI
                     MYMYDDESFSVDLMRFSKNTPRGVMDFMFINLIEYGKEKGYEIFNMGMAPLANVGLSK
                     YAFWNEKLALQFYENGQALYSFKGLRRFKEKFSHNWEYKYIAYRRNTSILITVIQAAI
                     VCSRNRNVDESIVIRNLKSLIK"
     gene            2654..3628
                     /locus_tag="K8M77_002109"
     CDS             2654..3628
                     /locus_tag="K8M77_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI7086575.1"
                     /translation="MKKLKWGILGPGNIARDFAQALNRVNGEVYAVASRNKERAEKFA
                     RENNVKKAYGSYDEIIKDKDIDVVYIATPHSNHYEYIIKSLNNNKHVLCEKAITVNER
                     ELEEALKIAREKNLILEEAMTLFHMPLYEKVIKKINKEDLGKVNMVQVSFGSFKEYDE
                     NNRFFNLDLAGGALLDIGTYALSFARYFLSSMPEEILSTVKKAKTGVDEKSGIILKTK
                     EDEIATISLAFRSKMPKRGIVSCDNGFITIDNFPRANKATINYLDGKVEVIECGEEEK
                     ALDYEVIFMEERIKENKESNSLELTYDVTKIMNKVRKDWGIFYPFENN"
     gene            3758..4531
                     /locus_tag="K8M77_002110"
     CDS             3758..4531
                     /locus_tag="K8M77_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI7086576.1"
                     /translation="MKSLVIKFISIFLGIICILYYILVNALSGKTTFSSFYLILGVVI
                     ILYTLFIDKLLKLQWFKNVYKPMKILALICISIFIIVEGAIIFYPKKSLKPCNYIVVL
                     GAGIRGENLTATLKDRLDKTIEYLEKTGFNGEIIVSGGQGPGESITEAYAMEKYLVEN
                     GVPKDKIVLENKATSTYENLNYSKKIIENLSGKPIKDLDILVVTTDFHAMRSNLLAKR
                     NGYDKAYLYTSKTQWYLVPSMYAREFFAFCKSYFLDKNI"
     gene            5085..>6411
                     /gene="mgtE"
                     /locus_tag="K8M77_002111"
     CDS             5085..>6411
                     /gene="mgtE"
                     /locus_tag="K8M77_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBI7086577.1"
                     /translation="MNKRITKEKLKDILDKYPSNKFDSLDDIHPADIVDLLEENEENI
                     NLIKKLPENMIADIIDFAKDDDKVNILKSLSEENKKKVLNLIASDELTDLMASLNREE
                     TSKLLSSMPVQDAKDLRKLLTYAPDTAGGVMATEFISIKDTMSVKETLEYLQLEAPKA
                     ESTYYIYVVDDLGILQGVVSLRELVISKFDKKISEIVNKNVINVPFDMDQEEVANIFD
                     KYGLLTIPVVDYKDMLLGIITIDDVIEIIKEETTEDIHRLGGVDGEEKVDSTVKESVK
                     SRLPWLFVNLITAFLASATVGLFEGTISRVVILATFMPIVAGMGGNGGTQTLTIIVRG
                     IALGELTFKNAKRIFFKEIGVGILTGAATGLVTAIVAVLWSQKPAIGIVIGLAMILNM
                     TAATFAGYLVPVVLKKLKVDPALASAVFVTTVTDVCGFFFFLGLGTLFLP"
BASE COUNT         2444 a          637 c         1139 g         2191 t
ORIGIN      
        1 tacttcagta tttaaaaatt tcaattcaga atatttcttt atgtatagaa ataaactaga
       61 ctttttaaac atattaataa ttgcagcatt aggtataatt tcttacattc ctttatcttt
      121 ctacgatttc attttaaaga gaaaagttag aataagatta aaaaatagaa agctatataa
      181 gtattcttgg atagcaagtt ctattgcaag tttgcttgga tttggaggag ctacatcctt
      241 agcttttaaa cagtattttt atggtgatta tgtagatgat aaaaagaaat tattaaaaga
      301 aattggtaaa atagtagcct taaacttaac aggcttatca atagtttgtt gtacatatat
      361 ggggattaga atatcttcgt ggaataatct gggtataata aaatatgcca taggaataat
      421 agccttatat gcgccaggat ttataattta ctcagcttat aaatatagca aaacaaagga
      481 caagttagag tttttcagca ccttaggtat tatattcata tcatttttag agtggcttac
      541 aacaataatt ttaatttatg aaactttaag aataacagga gcatcaataa gtgttttaaa
      601 tttcttgcca atatatattg aatcagcagt tgttggaatg atcagtatga ttccaggggg
      661 aattggtacc tttgatttaa cctttatgac tggattagag tccttaggta ttcctataga
      721 acaaacccta ttaggtataa tactttatag aataagttat tacatagttc cagcgcttat
      781 aggtgtatta ctttttgtac atgattttgg tggaaaaata aataaaaagt ttaatggatt
      841 accttatgaa atagtttcta aggtggctta taagatagtg gtttctttag tatttatatc
      901 aggtgctatt atagttttat caaatatagc acctcaatat ttattaaaga taaaattatt
      961 aaaagaaatt ttaggtaaac aagtattagg cttatcaatt ggaatgagtg tagtattagg
     1021 atttttaata atgcttgcag ctcttatgct aaaatatagg gctaaaagta tttacaaagc
     1081 aagtatggtt ttatttatat taggaataat attgtcctta acaaagggaa ttaatcctta
     1141 tgaattagta tttttaatta ttgtagctta ccttttatat ttaagtaaga gaatgttcta
     1201 cagggatagc tttgttgtaa gttgtaaaaa taccttaata gattcaggga tattaattgc
     1261 atctttttca atttatttct ttattttaat tacctttgga acacatctta aatatgtagg
     1321 aatagttcgt aaaatgcctt ataagatggc atataaattt ggatttatag cttttgcttt
     1381 agtaactgta atatatgtag ctatttactt tttaaatata agaagaaaaa ttcctgttaa
     1441 gacttttgat cagtgtagcg aatatataga aaagataata gaagaataca agggtgattc
     1501 cctaactcat ttagtatttt taaaggacaa gtatatttat ttaaatgaag ataaggatct
     1561 ttttattcaa tatgaagtct atggagataa gctttttgtt ttaggaaatc ctgtgggcaa
     1621 taatgaaaat ctatttaggg aaatagaaaa gttttgtgag tatactgata actatggtta
     1681 cacaccagtg ttttatcaag ttaatgaaga aatgataagt taccttcatt ctaatggata
     1741 tgattttatg aaaattggag aagaagctaa ggttgatgta aaagaattta aagttgtagg
     1801 aaataaaatg aagagtttaa aaacttcaag aagtaaggtg actaaggaag gttatacttt
     1861 ccacatggtt gaaccacctt tttcaagaga atttttagat tccttaaaag aaatatcaga
     1921 tgaatggtta gatggaagaa aagaaaaagg attttcagtg ggattctttg atgaagatta
     1981 tttaaataaa gctcctatag ctattttaag agatagagag ggagaaataa aagcctttgc
     2041 taatataatg tatatgtatg atgatgaaag cttctcagtg gatctaatga gatttagtaa
     2101 aaatactcca aggggagtta tggattttat gttcataaat ttaattgagt atggaaaaga
     2161 aaagggatat gaaatattca acatgggaat ggctccttta gccaatgttg gtttatctaa
     2221 gtatgctttc tggaatgaaa aattagccct tcaattctat gagaatggac aagcacttta
     2281 ctcctttaaa ggattaagac gttttaaaga gaaatttagt cataattggg agtataaata
     2341 tattgcttac agaagaaata catccatact aataacagta atacaagcag ctatagtttg
     2401 ttcaagaaat agaaatgtag atgaaagtat agttataaga aacttaaaaa gtttaattaa
     2461 ataaaaagtt acattttaat gggttgtatc tttagatccc tatttataca aaatttaatt
     2521 aagaataaaa tatatgattt caaaagggcc taaaataggt ccttttaatt tttaaataaa
     2581 ttttgcttta agtggattat tagtttaaaa taagcttaaa taatatactt tatattaaag
     2641 aggagctgag aatatgaaaa aacttaaatg gggaatatta ggaccaggaa atatagctag
     2701 agattttgca caggctttaa acagggttaa tggagaggtt tatgcagtag cttctagaaa
     2761 taaggaaaga gcagagaagt ttgcaagaga aaacaatgtt aaaaaagctt atggaagcta
     2821 tgatgagatt ataaaagaca aggatataga tgtagtgtat atagcaacac cacacagtaa
     2881 tcattatgag tacattataa aaagtttaaa taataataag catgttctat gtgaaaaggc
     2941 tataactgta aatgaaagag agttagaaga agctttaaaa atagcaagag agaaaaattt
     3001 aattttagaa gaggcaatga ccttatttca tatgccctta tatgaaaagg ttataaagaa
     3061 aataaataag gaagacttag gaaaagtgaa tatggttcaa gtttcctttg gaagcttcaa
     3121 ggaatatgat gaaaataaca gattctttaa tcttgattta gctggaggag cacttttaga
     3181 tattggaact tatgccttat cctttgcaag atatttctta tctagtatgc cagaggaaat
     3241 cttaagtaca gttaagaagg ctaaaactgg tgtagatgaa aagtcaggaa taatattaaa
     3301 gacaaaggaa gatgaaattg caactatatc cttagccttt agatctaaaa tgcctaagag
     3361 aggaattgtt tcctgtgata atggatttat aacaattgat aatttcccaa gagctaataa
     3421 ggcaaccata aactacttag atggaaaagt ggaagttata gagtgtggag aagaggaaaa
     3481 ggccttagat tatgaagtta tctttatgga agagagaata aaggaaaata aagaatctaa
     3541 tagcctagag ttaacatatg atgttacaaa aattatgaat aaggttagaa aagactgggg
     3601 aattttttat ccttttgaaa ataattaaaa atttattata aaacatcgga ataagcttag
     3661 taagttttta tatttacttt tgtaatatta ttgtatatat ttgcttttaa ggttttgata
     3721 aaatttttat attgagaact ggagaaagga gatatatgtg aagagtttag ttattaagtt
     3781 tatttcaatt tttttaggaa taatatgtat attatattat atcttagtta atgctttaag
     3841 tggaaaaaca acctttagct ctttttattt aattttagga gtagtaatta tattatacac
     3901 cctttttata gataaacttt taaaattaca gtggtttaaa aatgtgtaca agcctatgaa
     3961 gattttagct cttatatgca taagtatatt tataatagtg gagggagcaa taattttcta
     4021 tcctaaaaaa tccttaaagc cttgtaatta tatagttgtt ttaggggctg gaataagagg
     4081 agaaaattta actgccacat taaaagatag attagataag accatagaat atttagagaa
     4141 aactggattt aatggagaga ttatagtatc tggtggacaa ggtcctggag agagcataac
     4201 tgaggcttat gcaatggaaa aatatctagt ggaaaatgga gtgcctaagg ataaaattgt
     4261 acttgaaaat aaagcaacta gtacatacga aaatctaaat tattctaaga agatcataga
     4321 gaacttaagt ggaaagccta taaaagactt agatatttta gttgttacca cagattttca
     4381 tgctatgaga agcaatcttt tagctaaaag aaatggatat gataaggctt atttatatac
     4441 aagtaagact cagtggtatt tagtaccttc tatgtatgca agagaatttt ttgctttttg
     4501 taaatcatat tttcttgata agaatattta attttcataa atattaaaat tttaatcact
     4561 taatttaaat taggcatgag ttagtattaa aatttcgaat atagaattaa aatgaaaagc
     4621 agattaaaaa actaatctgc tttttattta gcatgtagtt atatgaatag atgtaaaagg
     4681 agaattacag cataaagaaa caaattatag attatatatt ttaagaatat tcataaaata
     4741 tactacatgc ttatttaaaa caagaaaaag tgaggttaaa aattatttaa ttataatttt
     4801 tattatatat ttatgagctg tttttataaa atattacaaa atataaaaaa ataataaaat
     4861 ttttatttac aaattgttta aatatactta atataatata tagaaataat attttgaaac
     4921 ttttatttca ttttggaggt ggtatgaatg gaaagcgttc cccttagaag gaactcaagg
     4981 ttaaaattaa aaataagagg gattgtacat ttatatgcct ttaaggagca aattatccta
     5041 tagagggttt gtattcctct tatttaagga aaagaggaaa tgatatgaat aaaagaataa
     5101 caaaagaaaa gttaaaggat attttagata aatatccaag taataaattt gattctctag
     5161 atgatataca tccagctgat atagttgatc ttttagaaga gaatgaggag aatattaatc
     5221 ttataaaaaa acttccagaa aatatgattg cagatattat agattttgcg aaggatgatg
     5281 ataaggtaaa tatattaaaa agtttatctg aagaaaacaa gaagaaggtt ttaaatttaa
     5341 tagcttcaga tgaacttaca gaccttatgg caagcttaaa tcgtgaagaa actagcaaat
     5401 tactctcaag tatgccggtt caagatgcta aggatttaag aaaattatta acatatgcac
     5461 cagatacagc tggtggtgtt atggctacag agtttatatc tataaaggat accatgagtg
     5521 taaaagaaac cttagagtac cttcagttag aagctccaaa ggctgaatca acttattaca
     5581 tatatgttgt agatgactta ggtatattac aaggggtagt ttctttaaga gaacttgtaa
     5641 taagtaagtt tgataagaaa atttcagaga ttgtgaataa aaatgtcata aatgttcctt
     5701 ttgatatgga ccaagaagag gttgcaaata tatttgataa atatggactt ttaacaatac
     5761 cagttgttga ttataaggat atgcttttag gtataataac aattgatgat gttattgaaa
     5821 ttataaaaga agaaacaaca gaagatatac accgtctagg tggtgtagat ggagaagaaa
     5881 aagttgatag cacagtaaag gaatctgtaa aaagtagatt accatggttg tttgtcaacc
     5941 taataactgc gttcttagct tctgccactg ttggactttt tgaaggaact attagtaggg
     6001 ttgtaatttt agctacattt atgcctattg ttgcaggtat gggtggaaat ggaggaactc
     6061 aaaccttaac aattatagta agaggtatag ctttaggaga attaactttt aaaaatgcaa
     6121 agagaatttt ctttaaggaa attggagttg gtattttaac aggggctgcc actggattag
     6181 taactgcaat agttgctgtt ttatggtcac aaaaacctgc aattggaata gtaataggat
     6241 tagctatgat attaaatatg actgctgcta cttttgcagg atatttagta ccagttgttt
     6301 taaagaaatt aaaagtagac ccagctttag cttctgctgt ttttgttaca acagtaactg
     6361 atgtatgtgg atttttcttc ttcttaggtt taggaacttt atttttacca t
//
LOCUS       DAEVGI010000140         6359 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.140, whole genome shotgun sequence.
ACCESSION   DAEVGI010000140 DAEVGI010000000
VERSION     DAEVGI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.140"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(17..1264)
                     /locus_tag="K8M77_002112"
     CDS             complement(17..1264)
                     /locus_tag="K8M77_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086578.1"
                     /translation="MNPFLEKSSKIQDYFTDWRNIYAKPYNKNEVDPYTKTRIILMNG
                     AEFEANWFSHQFSRNCNNNELRREIALARRLDKQQQMLISSLRPANESILETTISYEQ
                     LAVDLTARLAKREPNEHVKKALDFALLEDFDHLYRYSDLLFMEEGTKAENLVGHYTEI
                     MPGRPTIAHHRYPNDNIRNFVDFKTADLITKLDISIITAAEQQTMNYYMNIAGFYTSD
                     IGRNLYQEIGLIEEQHVSHYGSLLDPNCTWLENLLMHKYTEAYLYYSCYNSEVDPYIK
                     GLWEQCFVQEVTQLHKACDLLKKYENKEWQEVIPNGEFPELLTLGENISYVRDILDNT
                     VNNTTIKDDYVDVSKLGPDSSFHEFQNKVNKNVEDVPSHKVIVDFISKNNEDYRFETK
                     ENPILALRDRKSDNTSIGRTSLS"
     gene            1389..4592
                     /locus_tag="K8M77_002113"
     CDS             1389..4592
                     /locus_tag="K8M77_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7086579.1"
                     /translation="MNLDIFMKNLVRRTSSFTREQGKKLIKEAYVKDVKGKSIDGIYH
                     IYGSVLNDDKNWDYNTHIKINMQNSDIMGTNCSCETFKENSKHIKNYVCKHISATNDV
                     FYSLAKKKMQKNKLKSNNKPKLVKEKNEEHKGQEKRFLSLDINIKHMVKEGITLFNCE
                     FRIGAGNLNLILDLKDFLYKNSLKKPLKFNDGFTYNPLKDEFLDEDKRVLQFVASHKD
                     MISGRYLRLKQNNLKDFLKLIDEKKKINFNFNSINYEVKVKKENVPVALTLKEGKEGF
                     ILNHHKKFPIILNNSGDVMFFDRNLYLPRKRQLEYYIPIHKLFLKNNTITYKKSLENL
                     RSLLEELKNISKNIVLDENIRVFKEKLMKTTFNLYKNKEKIYCNVKIDYCGYIIDLIR
                     DEKDNSFLRDLKSEKYIEFQLERFKFIKREEDFCFIGSEEEIYELFSKGIKRLRELGE
                     VLLSEELKEFKVLDSSLISSELKELSNFYKLKFDFGDFELRELRESIEAMKRGDRFYR
                     TKKVYLDLEDPGIVNFLNLLDDLGLENIKDNEVYIDKSKVLYIQEKLKDRTLSFIKGG
                     NVLQEIVGKLLNKEFKRKLVPKALNAELRPYQKEGFKWINEITDLGFGGVLADDMGLG
                     KTLQIIAFLLSQKKSKSIVVVPTSVIYNWMDEFEKFAPSIRIGLVHGSKSKRDKVLRD
                     FKRGLGIKIEEKNLKEKSYEKYDVLLTTYGTLKNDEKAYENLSFDYCIIDEAQNIKNP
                     SAQATLSVKNIKSRCNIALTGTPIENNLMELWSIFDFVMPGYLFTKERFRERFILDES
                     NLSELKSLITPFILRRLKEEVLSELPEKLEKKYLVEMKGKQKQLYSFYVNAIKNQLNE
                     NKSSEKSGRDKINLFAYLTKLREICLDPSLVVPDYKGGSSKLTVVKEIVKDASESGKK
                     ILLFSQFTSVLQKIEEDFKKEDISYLYLDGGTSAKDRVERVKKFNEDSNIKVFLISLK
                     AGGVGLNLTSASVVIHFDPWWNPAVEDQATDRAHRFGQENKVEVIKLVAKDTIEEKIV
                     LMQEDKRELIQSLMDGKTMDGKGLKRLTEEEISKLFE"
     gene            5073..5759
                     /locus_tag="K8M77_002114"
     CDS             5073..5759
                     /locus_tag="K8M77_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBI7086580.1"
                     /translation="MRINKLFSNFGICSRKETNKIIREGRVIVNGKPCIEGQWVELSD
                     EILLDRKPLVPKEKIYIALNKPVGVVCTAAEEVKDNIIDYLGIDDYVFPIGRLDKDSQ
                     GLIILTNDGDLANEVLSAENNHEKEYIVTLDQPFYDDFIEKMEQGVEILGQVTKPCKL
                     KRINENTYSIILTQGLNRQIRRMSKVFGFNVVKLERIRILNITLEDIEYGKWRYINRE
                     EIEILKKLIK"
     gene            5774..>6359
                     /locus_tag="K8M77_002115"
     CDS             5774..>6359
                     /locus_tag="K8M77_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086581.1"
                     /translation="MGNDNRVKAWFTKLILILGSILIIYSIISDLYKISYLEFKGVET
                     TGKLITFNEKINSGRFKSKSYTPVVRIKVYGQEIDVPAKLNKIDKDLVDFVGDEVKVK
                     YSSKDYNLLIINDKKYLLNRINYMLLAVQILIFALLSICMKATCGEDGKVKWIIGKKN
                     PVLNLLFWTPGLVFSFSWIYYQIILGRYAVEQFDF"
BASE COUNT         2485 a          654 c         1076 g         2144 t
ORIGIN      
        1 tttttataag ttcatactaa cttaaagatg ttcttccaat agaagtatta tcagattttc
       61 tatctcttaa agcaagaatt ggattttctt ttgtttcaaa tctataatct tcattatttt
      121 ttgaaataaa atcaactatg accttatgac ttggaacatc ttcaacattt ttattaactt
      181 tattttggaa ttcatggaat gatgaatcag gacctaattt acttacatca acgtaatcat
      241 cttttatagt tgtattatta acagtattat ctaatatatc tctaacataa gatatatttt
      301 ctccaagtgt tagaagttct gggaattcac catttggaat aacttcttgc cattctttat
      361 tttcatattt tttaagaaga tcacaagctt tatgtaattg agtaacttcc tgaacgaagc
      421 attgttccca tagtccttta atatatggat caacttcaga attataacaa gaataataca
      481 aatatgcttc agtgtattta tgcataagta gattttcaag ccatgtacag ttaggatcta
      541 aaagacttcc atagtgagaa acgtgttgtt cttctattaa tcctatttct tgataaagat
      601 ttcttccaat atcactagta tagaaacctg ctatattcat gtaataattc atagtttgtt
      661 gctctgccgc agtaattatt gatatatcta gtttagtaat aaggtctgct gttttaaagt
      721 caacaaagtt tcttatgtta tcattagggt atctatgatg agcaatagtt ggtctacctg
      781 gcataatttc tgtataatgt ccaactagat tttctgcttt tgttccttct tccataaata
      841 ataaatctga atatctatat aaatggtcaa aatcctcaag taatgcaaaa tctaaagctt
      901 ttttaacatg ttcattaggt tcacgctttg caagtctagc agttaaatct acagctagtt
      961 gttcataact tatagtagtt tctaaaatac tttcattagc aggtcttagt gaactaatta
     1021 gcatttgttg ttgtttatct aatcttctag caagggcaat ttctcttcta agttcattgt
     1081 tattacaatt tctagagaat tgatgagaaa accagtttgc ttcaaattca gctccattca
     1141 ttaaaattat tctagttttt gtataaggat caacttcgtt tttattataa ggttttgcgt
     1201 aaatatttct ccagtctgta aaataatctt gtatttttga gcttttttct aaaaatggat
     1261 tcataataaa taaatcacta cctttccaaa ttttaatata attttagtat tacaaaatta
     1321 ttttttagta ttcattaatt tgtaattatg ttagaataat tctattaaaa gcaaggagtg
     1381 gtatgccttt gaacttagat atttttatga agaacttagt aaggcgaact tccagtttta
     1441 ctagagaaca aggtaaaaag ttaattaagg aagcatatgt taaagatgtt aagggtaaga
     1501 gcatagatgg aatttatcat atttatggaa gtgtactaaa tgatgataaa aactgggatt
     1561 ataatacgca cataaaaata aatatgcaaa atagtgatat tatgggtact aattgtagtt
     1621 gtgaaacctt taaagagaat agtaaacata taaaaaatta tgtgtgtaaa cacatttcag
     1681 caactaatga tgttttttat tccttagcaa agaagaagat gcaaaagaat aaattaaaaa
     1741 gtaataataa gcctaaatta gttaaagaga aaaatgagga acataaaggg caagaaaaga
     1801 ggtttttatc tttagatata aatattaaac acatggtaaa agagggaatt actttattta
     1861 actgtgagtt tagaattggt gcaggaaact taaatttaat actggattta aaagatttct
     1921 tatataaaaa tagcttaaaa aagcctttaa aatttaatga tggatttact tataatccat
     1981 taaaagatga atttttagat gaagataaaa gagttcttca atttgtagca agccataagg
     2041 atatgataag tggaagatat ttaagactta aacaaaataa tctaaaagat ttcctaaaat
     2101 taattgatga aaaaaagaaa ataaatttta attttaactc aattaattat gaggttaagg
     2161 tgaaaaagga aaatgttcca gtagctctga ctttaaagga aggtaaagag ggatttatat
     2221 taaatcacca taagaaattt cctattattt taaataactc aggagatgta atgttttttg
     2281 atagaaattt atatcttcca aggaagaggc agttagaata ttacattcca attcacaagt
     2341 tatttttgaa aaataacaca atcacatata agaagagctt agagaattta agaagtctat
     2401 tagaagaact taaaaacata agcaagaata tagttttaga tgaaaatatt agagttttta
     2461 aagaaaagct tatgaaaaca acctttaatt tatataaaaa taaggaaaaa atttattgta
     2521 atgtaaagat tgattattgt ggatacataa ttgatttaat aagagatgaa aaagataata
     2581 gctttttaag agatttaaaa agtgagaagt atatagaatt tcaacttgaa agatttaagt
     2641 tcataaagag agaagaagat ttttgcttta taggaagtga agaggaaata tatgagcttt
     2701 tctctaaggg aattaaaagg cttagagaac ttggagaagt tttattatca gaggaactta
     2761 aggagtttaa ggttttagat tcatcactta tttcatcaga acttaaagag cttagtaatt
     2821 tctataaact taagtttgac tttggagatt ttgaattaag agaacttagg gaaagcatag
     2881 aggctatgaa aaggggagac cgcttttata gaactaagaa agtttattta gatttagaag
     2941 atcctggcat agtaaacttt ttaaatcttc tagatgattt aggattagaa aatataaaag
     3001 ataatgaagt ctatatagat aagagcaagg ttttatatat tcaagaaaaa ttaaaggata
     3061 gaactctctc ttttataaaa gggggaaatg ttcttcaaga aatagtagga aagcttctaa
     3121 ataaagaatt taagagaaag cttgttccaa aggctttaaa tgcagagctt agaccttatc
     3181 aaaaagaggg ctttaaatgg attaatgaaa ttactgattt aggttttggt ggagttttag
     3241 cagatgatat gggacttggt aaaacccttc aaataatagc ttttttatta tctcagaaaa
     3301 aaagcaaaag catagtagtt gtcccaacat ctgttatata taactggatg gatgagtttg
     3361 aaaagtttgc tccaagcata aggattggat tagttcacgg tagtaaatct aagagagata
     3421 aggttttaag ggattttaaa agaggacttg gtattaagat agaagagaag aatttaaaag
     3481 aaaaatccta tgaaaaatat gatgttttat taactaccta tggaacctta aagaatgatg
     3541 aaaaagccta tgaaaactta agttttgatt attgcataat agatgaggct cagaatataa
     3601 agaatccatc agcacaagca actttatctg ttaaaaatat taaatcaaga tgcaatattg
     3661 ccttaacagg aactcctata gagaataatt taatggagct ttggtcaata tttgattttg
     3721 ttatgcctgg atatttattt actaaggaga gatttagaga aagatttatt ttagatgaaa
     3781 gcaatttaag tgaattaaaa tccttaataa ctccttttat tttaagaaga cttaaggaag
     3841 aggttttaag tgaacttcca gaaaaacttg agaaaaaata cttagtagaa atgaaaggaa
     3901 aacaaaaaca gttatatagc ttctatgtaa atgcaataaa gaatcaatta aatgaaaata
     3961 aaagttctga aaagagtgga agagacaaaa ttaatctatt tgcctatttg acaaaattaa
     4021 gagaaatttg tttagatcct tcactagttg taccagatta caagggagga agtagtaagc
     4081 ttactgtggt aaaagaaatt gtaaaagatg ctagtgaatc aggaaagaag attttactat
     4141 tttctcaatt tacttcagta ctacaaaaaa tagaagagga ctttaaaaaa gaggatattt
     4201 cttatttata tttagatgga ggaacttctg ctaaggatag agtagagaga gttaaaaaat
     4261 tcaatgagga tagtaatatt aaagtgtttt taatatctct aaaggctggg ggagttggac
     4321 taaatttaac ctctgccagt gtggttatac attttgatcc ttggtggaat ccagccgtag
     4381 aagaccaagc tacagatagg gcacatagat ttggacaaga aaataaagtt gaagttataa
     4441 agttagttgc aaaggatact attgaggaaa aaatagtatt aatgcaggag gataaaagag
     4501 aacttattca aagtttaatg gatggaaaaa ctatggatgg taagggatta aaacgactta
     4561 cagaagaaga aattagtaaa ttatttgagt agggatttaa taaaagaaaa aaagctaaga
     4621 tgtctaatca ccttagcttt ttttctaatc ttactattat ctgtagttgg tggcaaacca
     4681 agttagagag tttaaagaaa taagactaaa attaatctta tttatacata aaaatcatcc
     4741 aagttatttt taaagcaaag aaggttgttt actctaaaaa tacaatataa aaacaggcta
     4801 acagaaaatc cgaaaagttt atagtataac catacgttcc taaagtatgt tattaaaatt
     4861 taaatatact ataattatat tataaactac tatttgcaat atataaacta atttttaaaa
     4921 tttggttaaa ataaatattt ttactaaaga aaaaagaata tttctgtgct aattttgaaa
     4981 gttattcagt aattctgtta taattaagca tatgaattta gaactaatat aaataagaat
     5041 ttaactttag gaaggaatgt ttataattac taatgagaat aaacaagctt tttagcaatt
     5101 ttgggatatg ctctagaaaa gaaactaata aaattattag agagggaagg gttattgtta
     5161 atggcaagcc atgtattgag gggcaatggg ttgaattaag tgatgaaata cttttagata
     5221 gaaaaccctt agttccaaag gaaaagattt atatagcact taacaagcct gtaggagttg
     5281 tatgtactgc tgcagaagaa gttaaggata atataataga ttatttaggg atagatgatt
     5341 atgttttccc aataggaagg ttagataagg actctcaagg attaataatt ttaactaatg
     5401 acggagattt agcaaatgaa gttttatcag cagaaaataa ccatgaaaag gaatatatag
     5461 taaccttaga tcaacctttt tatgatgatt ttattgaaaa gatggagcag ggtgttgaaa
     5521 tattaggaca agtaacaaaa ccttgtaaac taaagagaat aaatgaaaat acctattcaa
     5581 taatattaac tcaagggtta aatagacaaa taagaagaat gagtaaggtt tttggcttta
     5641 atgtagttaa gttagagaga attagaattc ttaatataac tcttgaagac atagaatatg
     5701 gaaagtggag atatataaat agggaagaaa ttgagatttt aaaaaaactt ataaagtagt
     5761 taaggggata aaaatgggaa atgacaatag agtaaaagca tggtttacaa aattaatact
     5821 aattttagga tctatattaa ttatttattc aatcatatct gatttgtata aaattagtta
     5881 tcttgaattt aaaggcgtag aaacaacagg aaagttaata acttttaatg aaaagattaa
     5941 ttcgggaaga tttaaatcaa aatcatatac tcccgttgta aggataaagg tttacggaca
     6001 agagattgat gttcctgcaa agttaaataa aattgataaa gacttagttg actttgttgg
     6061 agatgaagtg aaagttaaat attcttctaa ggattataat cttttaatta taaatgataa
     6121 aaaatatctt ttaaatagaa ttaattacat gctactagca gttcaaattt taatattcgc
     6181 tttattatct atttgtatga aggctacttg tggagaagat gggaaagtaa agtggattat
     6241 tgggaagaaa aatcctgttt taaatttgtt gttttggaca cctggtttag tattttcatt
     6301 ttcatggatt tattatcaaa taatattagg gaggtatgcc gtggaacagt ttgacttta
//
LOCUS       DAEVGI010000141         6253 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEVGI010000141 DAEVGI010000000
VERSION     DAEVGI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.141"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(208..486)
                     /locus_tag="K8M77_002116"
     CDS             complement(208..486)
                     /locus_tag="K8M77_002116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086582.1"
                     /translation="MIDIIIIILLFIAIGIGMVLLINSLKKKYKTRNQDEQSTNEDYM
                     SLGMLLGMCFGVAIGSMFTNKFGTNSISYGVSFGMLGGILIGMNIKKK"
     gene            complement(722..1510)
                     /locus_tag="K8M77_002117"
     CDS             complement(722..1510)
                     /locus_tag="K8M77_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBI7086583.1"
                     /translation="MIKFIATDMDGTLLNSKKELSPEFYDVFEELKKRDILFAAASGR
                     QYYTLAKEFNDIKDDMLFIAENGTFVVYKGKELVVNGLDRELANELIRIGRTIENADV
                     VLCGKNSAYVESSDERFLGEVRKYYERCEIVDDLEKVDDTVLKVTMCDFNGSEENSNK
                     YFDKYRDELQVTVSGDIWLDITAGGVNKGVAIKEIQDLLDIDFEETMVFGDYLNDLEM
                     MESAYHSYAMENAHEDLKKVSRFITHKNNDEDGVMHQIKEVIGL"
     gene            complement(1629..2393)
                     /locus_tag="K8M77_002118"
     CDS             complement(1629..2393)
                     /locus_tag="K8M77_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI7086584.1"
                     /translation="MFMEERLEEILRILKNDGKVLVKDLSIKFNVTEGMIRKDLQKLE
                     QEGLLKRTYGGAILERQTIHDDNIRPRLMKNLGEKDIIAKLALNEIKDGDFMFLDAST
                     TNYSIASMLLNTKKQVTLVTNMNRIAILFDSHSSIDVICIGGIYNKNLGGTIGSKAIE
                     DVKSYRFHKAFVGVGGINLVEDFISNFNLEEASMKNAIIKSSLKTYLVAENEKFYTDG
                     ICEFAKCEDIDFIISDKKPNEEILKLLKERNISIIY"
     gene            complement(2653..3828)
                     /locus_tag="K8M77_002119"
     CDS             complement(2653..3828)
                     /locus_tag="K8M77_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI7086585.1"
                     /translation="MFFNDKKLKGALIVVTYAIILHFVFMKYSTVFGGLGYITGLVKP
                     LILGIAIAFVLNIPMKFFERKLFRNLENSKKSWVRSAKRPLSIAVTIIVLIGTISAII
                     LFVVPQLTSSVVKLTESIPTYLKSFEVNMNNYADSTVLLSKVWDQVMNTWQEILKTVT
                     HLFGGIASYIVGLTVDVTTAIIDFFLAFVLAIYMLSSKEKLICQIKKVMYAYLRGDIA
                     DKILEIGQTTNRVFSGFIIGQCTEALIIGTLCFLGMLVLRMPYAFLISVLVGTTSLIP
                     ILGAFIGTIPSFFLIFIINPIKAFWFIVFIFCLQRVEGDLIYPRVVGGSVGLSSLWVM
                     LAIMIGGSAFGFIGMLVGVPTAAVIYCVFGSVTNKRLSKKGIKIDEEEIEDSGKIHV"
     gene            complement(3978..4982)
                     /locus_tag="K8M77_002120"
     CDS             complement(3978..4982)
                     /locus_tag="K8M77_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI7086586.1"
                     /translation="MALMSDKFLSIVIPAYNSEKYIEKNLMFLSRQTSNNFEVIVVDD
                     GSTDNTCEVSETCLENFKIDHRVIRCEENRGQSVARNIGINYSRGKYILFLDSDDFAE
                     NNLVQILERNMFNEPDIVFFDYKRIKEDDSVNVNIAQNFEFGKIKSGEEVFNAYKDNK
                     IRLWTGSLVYNKEFLNKNKLRFLEGAHGAEDLNFIFKALLSSKKVRGIEDSLVFYCQR
                     GDSLTNNPDIYKNITVVESMEDVAKFIDENKLDKKLKEAIEKEFTCEHIMYQILGYLN
                     SETKKDTLSVIKNKNVKKYLKKATIKTNRYGRSMYTYMKIAAYFPNLFIKTYLKNTGK
                     "
     gene            complement(5037..6239)
                     /locus_tag="K8M77_002121"
     CDS             complement(5037..6239)
                     /locus_tag="K8M77_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBI7086587.1"
                     /translation="MTKLYYEDQYIKEFKGEIIEVKEIDGKFHVLLDQTAFFPGGGGQ
                     MGDLGLIDGIKVLDVYEEEGKVYHILEKEPKKFKNLQCELDWERRFDGMQQHLGQHLL
                     SGCFYDLFGANTCGFHLGKEISTVDIVGFLDEKTIREAEKEANRLIFENLEVKSYAPS
                     KKELKKVKTRRALPKTDEEIRIVEIVGLDLNACCGVHPRNTRDLQVIKIRRWEKHKNA
                     TRIEYVAGNRAVSDFFTKDEILGEICKLLKSGEGDTLNAVKNLLENNKNLVDENRKVK
                     AEIGDYKIKEMLNKSERIGSITLVNEIFDGEDTKHVGKLANKITEEYEAIVLFAVKNG
                     DRVNLIFNSSKDMKKVNMSDILKDTITLIDGRGGGNQFAAQGGGKNNGNTEVAIDYAT
                     NKIRNILL"
BASE COUNT         2081 a         1124 c          521 g         2527 t
ORIGIN      
        1 ctaaatataa aaaaataata gtagtaaaca ctactattat tcttaatttt catatataaa
       61 atgtattata tttgggttat gttttaatat aatgttgatt tataaactat tcaaataacc
      121 caaaaaggta ggacatattc atataatcct accttcaaat tagcattata ttaagtttgc
      181 taatatatta ttctgcttaa taaagcatta ctttttcttt atgttcatgc ctataagaat
      241 tccacctaac ataccaaaag atactccata agaaatagaa tttgtaccaa atttattagt
      301 aaacattgaa ccgattgcta caccaaagca cattcctaat aacattccta atgacatata
      361 atcttcgttt gtactttgtt cgtcttggtt tcttgtttta tatttcttct taagactgtt
      421 tattaataaa accattccaa ttccaatagc tataaatagc aaaataataa ttataatatc
      481 aatcatgatt ttagctccta taatttgttt agtttactat attaaaataa ttataaattt
      541 tatttcttat tatataacaa ttgcgtgctt actttttatt atagcattct tttaaccaat
      601 ttattaccag ttacataaac tatggggctc ttaatttgaa atccttataa gatagtaatt
      661 cctctacttg atttaatggt attatttgta tagttgcttt aatacaactc tttacttaat
      721 tctataatcc tataacttct tttatttgat gcataactcc atcttcatca ttatttttat
      781 gagttataaa tcttgaaacc ttctttaaat cttcatgagc attttccatt gcataactat
      841 gataagcact ttccatcatt tctaaatcat ttaaataatc tccaaatacc atagtttctt
      901 caaagtctat atctaaaaga tcttggattt cttttatggc cactccttta ttaactcctc
      961 cagcagttat atctaaccat atatcccctg aaacagttac ttgaagctca tctctatact
     1021 tatcaaaata tttattgcta ttttcttctg aaccattaaa atcacacata gtaactttta
     1081 aaactgtatc atctaccttt tctaaatcat ctactatttc gcatctttca tagtattttc
     1141 taacttctcc taagaatctt tcatcactac tttcaacata ggctgaattt tttccacata
     1201 aaactacatc tgcattttct atagttcttc ctattcttat aagttcatta gctaattccc
     1261 tatctaatcc attaacaaca agctcttttc ctttataaac aacgaaagtt ccattttcag
     1321 caataaataa catatcatct tttatatcat taaactcttt agctaaagta taatattgtc
     1381 ttccacttgc agctgcaaaa agtatatctc ttttctttag ttcctcaaat acatcataga
     1441 attctggact taactctttc ttactattta ataatgttcc atccatatct gttgctataa
     1501 attttatcat atatatacat cctcctaagt aaaagtataa attaaagtta attttatgtt
     1561 caattctata tatttaatat aaattctaaa ataatcaaag tataaattta gatgtaaagt
     1621 attaagtcct agtatattat agaaatattt ctttctttta aaagctttaa aatttcttca
     1681 ttaggctttt tatcacttat tataaagtca atatcctcac atttagcaaa ttcacaaata
     1741 ccatcagtat aaaacttttc attttcagca actaaataag tttttaaact agatttaatt
     1801 atagcatttt tcatactagc ttcttctaag ttaaagttac ttatgaagtc ctctactaaa
     1861 tttattcctc caacccctac aaatgcctta tgaaatctat aacttttaac atcctctata
     1921 gctttacttc ctatagttcc ccctaagttt ttattataaa taccaccaat acaaattaca
     1981 tctatagatg aatgtgaatc aaaaagtatt gctatcctat tcatatttgt tactaaagtt
     2041 acttgctttt ttgtgtttaa aagcatacta gcaattgaat agtttgttgt tgatgcatct
     2101 aaaaacataa aatctccatc tttaatttca tttaatgcta attttgctat aatatccttt
     2161 tcacctaagt ttttcattaa tctaggcctt atattatcat catgaatagt ttgtctttct
     2221 aatatagctc caccataagt tctttttaat aatccttctt gctcaagttt ttgtagatct
     2281 tttcttatca ttccttctgt aacattaaat ttaatactta aatctttaac taaaactttt
     2341 ccatcgtttt ttaatattct taagatctct tctaatcttt cttccataaa cattacaata
     2401 taccgctatt cctttacgct taagtaaccc cttattaata agaacaatct tttattattt
     2461 agtatctttt attatcattt attcttattt attattatat tcttttatgt ttaaaaaagc
     2521 aataatgaat aaaaacctcg ttgaaatagc cctttaaatc agcaaaggtt taagataata
     2581 tatgcaaaaa taaaaagaag gaattatatc tactttttaa taaagtcttg ataaaattcc
     2641 ttcttttatt atttatacgt gaatttttcc actgtcttct atttcttctt catctatttt
     2701 tatacctttt ttgcttaatc tcttattagt aacacttcca aatacgcagt atataacagc
     2761 cgctgttgga actcctacta acattccaat aaacccaaag gcactaccac caatcataat
     2821 tgcaagcatt acccataaag atgataatcc tacagatcct cctacaactc ttggataaat
     2881 taaatctcct tcaactcttt gaagacagaa tataaataca ataaaccaaa atgccttaat
     2941 aggatttatt ataaatatta agaaaaatga aggtattgtt cctataaatg ctcctaaaat
     3001 aggaattaaa cttgttgtac caacaagaac acttattaag aaagcatatg gcattcttaa
     3061 tactaacata cctaaaaagc ataaagtacc tattatcagt gcttcggtac attggccaat
     3121 aataaagcct gaaaatactc tattagtagt ttgtccaatt tctaatattt tatctgctat
     3181 atctcctcta agatatgcat acataacctt ctttatttga catattaatt tttccttgct
     3241 tgaaagcatg tatatagcta atacaaaagc tagaaaaaaa tcaattattg cagttgttac
     3301 atcaactgtt aagccaacaa tataagatgc aattccacca aataaatgag taactgtttt
     3361 taatatttct tgccaagtat tcataacttg atcccaaact ttacttaaaa gtactgtgga
     3421 atcagcgtaa ttattcatat taacttcaaa ggattttaaa taagtaggaa tactttctgt
     3481 aagtttaact acacttgaag ttagttgagg aacaacaaat aaaattatgg cacttatggt
     3541 accaattaaa actattattg ttaccgcaat agataaaggt ctttttgcac ttctaaccca
     3601 ggatttttta ctattttcta aatttctaaa taactttctc tcaaaaaact tcataggtat
     3661 atttaataca aaggctattg ctatacctaa aatcaatggt ttaacaagcc ctgtaatata
     3721 gcctagtcct ccaaatactg tagaatattt cataaataca aagtgtaaaa taatagcata
     3781 agtaacaact attagggctc cttttaactt tttatcattg aaaaacatat tttcacttcc
     3841 ttattttaag atatttttca tcttttcaaa aataatttta ttattaatga aaataactta
     3901 tatctactta aatataccat attgttaact ttttgttaat aacattttct tttatactta
     3961 atttaatata tacttctcta ttttcctgta ttttttaaat atgtttttat aaataaattt
     4021 ggaaaatatg cagctatctt catataagtg tacatacttc ttccatatct atttgtttta
     4081 atagttgctt tttttaagta ctttttaaca tttttatttt taataacact taaagtatct
     4141 ttctttgttt cactatttaa atatcctaat atttgataca taatatgctc acaagtaaat
     4201 tctttttcta tagcttcttt aagcttttta tctaatttat tttcatcaat aaatttagct
     4261 acatcctcca ttgattctac tacagttata ttcttgtata tatcaggatt attagttaaa
     4321 gaatctcccc tttggcaata aaacactaaa ctatcttcta ttcctctaac cttctttgaa
     4381 cttaaaagtg ccttaaatat aaagtttaaa tcctctgctc catgagcacc ttctaaaaat
     4441 ctaagtttat ttttatttaa gaactcctta ttgtagacaa gacttccagt ccaaagtctt
     4501 attttattat ctttataagc attaaagact tcttcacctg attttatttt tccaaactca
     4561 aagttttgag ctatattcac atttacacta tcatcctctt tgattctttt ataatcaaaa
     4621 aatactatat caggttcatt aaacatattt ctttctaaaa tctgaactaa attattctct
     4681 gcaaaatcat ctgagtctaa aaataagata tattttcctc ttgaataatt aattccaata
     4741 tttctagcaa cactctgccc tctattttct tcacatctaa tcactctatg atctatttta
     4801 aagttttcta aacaggtttc acttacttca catgtattat ctgttgaacc atcatcgaca
     4861 actattactt caaaattatt tgatgtttgt cttgataaaa acataagatt tttttcaata
     4921 tacttttctg aattatatgc aggaattact atacttaaaa acttatcact cattaatgcc
     4981 atctcacttc aactccatta tagtttttaa attaagctta taaatagttt taaaatttaa
     5041 agtaaaatat ttcttatttt attagtagca taatcaattg caacttctgt atttccatta
     5101 ttttttcctc caccttgagc tgcaaactga tttccgccac ctcttccatc aattaaagtt
     5161 atggtatctt ttaatatatc actcatatta acttttttca tatctttaga actattaaaa
     5221 attaaattta ctctatctcc atttttaact gcaaataaaa ctattgcttc atattcctca
     5281 gttattttat tagctaactt tcctacatgt tttgtatcct caccatcaaa aatttcatta
     5341 actaaagtta tacttcctat tctttctgat ttatttaaca tctcttttat cttgtaatca
     5401 cctatttcag ctttaacttt tctgttttca tctacaagat ttttattatt ttctaaaaga
     5461 ttcttaacag cgtttaaagt atcaccttct cctgatttta aaagcttgca tatttctcct
     5521 aaaatttcat ccttagtgaa aaagtcagat acagctctat ttccagctac atattctatt
     5581 cttgtagcat ttttatgctt ttcccatctt cttatcttta taacttgaag atctctagta
     5641 tttcttggat gaactccaca acaagcattt aaatctaatc ctacaatttc aacaattctt
     5701 atttcttcat cagttttagg caatgctctt ctagttttta ctttctttaa ttcttttttg
     5761 cttggagcat aactttttac ttctaaattc tcaaagatta atctattcgc ctctttttca
     5821 gcttccctta tagttttttc atctaaaaaa cctacaatat caactgtact tatttctttt
     5881 cctaaatgaa aaccacaagt atttgcacca aataaatcat aaaaacatcc agaaagtaaa
     5941 tgctgcccta aatgctgttg cattccatca aatcttcttt cccaatctaa ttcacattgt
     6001 agatttttaa atttcttagg ctctttttca agaatatgat atacttttcc ttcttcttca
     6061 taaacatcaa ggactttaat tccatcaata agtcctaaat ctcccatctg tcctccccct
     6121 cctgggaaaa aggcagtttg gtctaaaaga acatggaatt tcccatcaat ctctttaacc
     6181 tcgattattt ctcctttaaa ctcctttata tattgatcct cataataaag ttttgtcatt
     6241 ttatatcaac acc
//
LOCUS       DAEVGI010000142         6249 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEVGI010000142 DAEVGI010000000
VERSION     DAEVGI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.142"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            24..1721
                     /locus_tag="K8M77_002122"
     CDS             24..1721
                     /locus_tag="K8M77_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7086588.1"
                     /translation="MKKKSVGITMAVFLLGIFMGAIDSGIVSPARDIIANGLKVSQNA
                     SVWVVTIYTLAYAVSMPLIGKLSDKYGRKKIYMVSITLFGLGSLLCGISDYVNSYTFL
                     LFSRVIEAVGGGGIMPIATAYIGTSFPVEKRGSALGMIGGVYGIATVVGPTLGSGILS
                     IFGDKNWGFLFLVNVPISIIILLMATKLEENTSAQGIKKLDVCGSGVLTILILSLMYG
                     ATNLKFYDFANSIKSLDVWPYLLIFIISIPILVLVEKKAEDPVINLSYFTNKEIAITL
                     ILSFVVGCGLMATVFIPQFSENILRTPMGSGGYIVTIFAIFVGIAAPLGGKFIDKIGV
                     KKVLLIGMSLVIIGNLYQGYVTTKHPGMVNLIIGLAIMGFGLGFSMGTPINYLMLSLV
                     PDNEATVGQSAVSLIKSIGIAVSPNILINFISDAGRRVPGALQKVMPHIDGMSNIMSN
                     SGGASNFNNSMANASVTNIFSLIKEMVQSQFAALGDKFSNNPHMNIGMIEKSYMQSLD
                     GAKGAIETAFQKTMNTGYTKLFVTCAIIALIGLILTAMLNNNLITMKNRRLEKKEKN"
     regulatory      1772..1875
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2001..2195
                     /gene="thiS"
                     /locus_tag="K8M77_002123"
     CDS             2001..2195
                     /gene="thiS"
                     /locus_tag="K8M77_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBI7086589.1"
                     /translation="MIVNGKKVEKGELNTVYDLLKSLNVNSKRVVVELNKEIVNSCNY
                     NEILLNHEDVIEIIAFVGGG"
     gene            2272..3075
                     /gene="thiF"
                     /locus_tag="K8M77_002124"
     CDS             2272..3075
                     /gene="thiF"
                     /locus_tag="K8M77_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HBI7086590.1"
                     /translation="MNIKINEKWRDVKENCTLYSLKNEEFPDSHVIVLNGFPLVEDKD
                     LKEGDRVVFIKKGIMPNKEELEELMVSRHTPGIHEKLKKIKVLIAGCGGLGSNIAISL
                     GRIGIGSISLVDFDIVEPSNLNRQQYYIEDIGEFKVIALKRNLIRINPFIKIEALNLK
                     LSEDNMGILQGADIIIEAFDNPDYKALLANYVLSNMKDKFLISSSGMAGFYDSNLIKT
                     KQITDKFYICGDLVNEAKEGCGLMAPRVAICANHMANLVIDIAVNKIEF"
     gene            3205..3969
                     /locus_tag="K8M77_002125"
     CDS             3205..3969
                     /locus_tag="K8M77_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBI7086591.1"
                     /translation="MDKLIIGGVEIKNRLFVGSGKYPSNEIIKDVLEGSGSQVITLAL
                     RRVDLDNKEEDILQNIPKDVILLPNTSGATNAEEAIRIARIARAMGCGNWIKIEVISD
                     SKYLLPDNEETIKATKVLADEGFIVLPYMCPDLYAGRRLIEAGAAAVMPLGAPIGSNR
                     GLKTKELIQIMIDELDIPIIVDAGIGKPSQAMEAMEMGAAACLVNTAIASSEDPINMA
                     RAFKMAVEGGRLAYEAKMGRESKFGNASSPLTGFLD"
     gene            3982..5085
                     /gene="thiH"
                     /locus_tag="K8M77_002126"
     CDS             3982..5085
                     /gene="thiH"
                     /locus_tag="K8M77_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBI7086592.1"
                     /translation="MSFYDVVEKYRDFDFYGYFDSVKKEDVLRSIYERNKRPEDLLNL
                     ISPMGELVLEEMAQEARNLSLKYFGRTILLYTPMYISNYCVNKCSYCGYNVENKICRK
                     KLNQEEIEKEGEAISKEGFKHILILTGESEYHTPVEYIEKSIKTLKGKFPSITIEIYP
                     MTEEGYKKVVEAGAEGLTVYQETYDEKVYDRVHVAGPKKNYKFRLEAPERGAEAGMRS
                     ISIGALLGLADFRIDAFFTAMHGKYLRDKYPHIDISYSVPRIRHCEGGLKKLNEVYDR
                     ELVQILLAYRLFDPQGGINISTREGKDFRRNLIPLGVSKISAGVSTEVGGHSLKEKGT
                     SQFDINDESSVSEVKELIKSEGYQPIFKDWHRF"
     gene            5115..5696
                     /locus_tag="K8M77_002127"
     CDS             5115..5696
                     /locus_tag="K8M77_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBI7086593.1"
                     /translation="MFLITNRKLVNREKYFNTIEEAGKYGVKNIILREKDLSTEKLIE
                     VYIKIKELVPEETNIIINSNIEAAKILKEKFIHLSFKDFKKNLEEVKSLEVGVSVHSI
                     LEAIEADRLGASYILVSPIFETQCKKDVTPKGINFIKEIKEKVNCKVIALGGINELNF
                     KEVLGAGADDFACMSLLFMSNNIKECLDTFKSL"
     gene            5808..6239
                     /locus_tag="K8M77_002128"
     CDS             5808..6239
                     /locus_tag="K8M77_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBI7086594.1"
                     /translation="MSFERIDISDRSEQEGRSCVILYNFENKELKKIQNFARIMGIKD
                     QIVLNSKNGDETIKNILEDKIEKTEGEGVKERAIIFNAIAPGKVHMFLENLKKIGIQR
                     PLVAMVTETSINWTIDVLVKNLIAERRALRSGDMTTHENKF"
BASE COUNT         2444 a          623 c         1226 g         1956 t
ORIGIN      
        1 aaacttacga aagggtgata ataatgaaga aaaaatcagt tggaattaca atggctgtct
       61 tcttgttagg aatcttcatg ggtgctattg actctggtat agtttcacca gcaagagata
      121 taatagctaa tggattaaaa gtttcacaaa atgctagtgt ctgggttgta acaatatata
      181 ccttagcata tgcagtatca atgcctctta taggaaaact ttctgataaa tatggtagaa
      241 aaaaaattta catggtttca ataactctat ttggactagg ttctttacta tgtggaatat
      301 cagattatgt aaatagttat acattcttat tattttcaag agttatagaa gcagtaggtg
      361 gcggaggtat aatgccaata gctacagcgt acataggaac atcattccca gttgaaaaaa
      421 gaggttcagc gctaggaatg attggagggg tatatggaat agcaacagtt gtaggaccaa
      481 ccttaggttc aggaatactt tctatctttg gagataaaaa ctggggattt ttattcttag
      541 taaatgttcc aatcagtata ataatattac ttatggcaac taaattagaa gaaaatactt
      601 ctgcacaagg aattaagaaa ttagatgttt gtggttcagg agtattaaca atattaattt
      661 tatctttaat gtatggagct acaaacttaa aattctatga ttttgctaat tcaataaaat
      721 cactagatgt ttggccatat cttttaatat ttataatatc aattccaata ttagttttgg
      781 tggaaaagaa agcagaagat ccagtaataa acttatctta ctttactaat aaggaaatag
      841 ctataacttt aatattaagt tttgttgtag gttgtggatt aatggcaaca gtatttattc
      901 ctcaatttag tgaaaatata ttaagaacac caatgggtag tggtggatat atagttacaa
      961 tatttgcaat atttgtaggt atagcagcac ctttaggtgg aaaattcata gataaaatag
     1021 gagttaaaaa agtactatta ataggtatgt ctttagttat aataggtaac ctttatcaag
     1081 gatacgtaac aactaaacac ccaggtatgg ttaacttaat aataggttta gctattatgg
     1141 gatttggctt aggattctct atgggaacac caataaatta cttaatgctt agtttagtac
     1201 cagataatga ggctacagtt ggacaatcag cagtatcatt aattaaatcc ataggtattg
     1261 cagtatcacc aaatattctt attaacttta tatcagatgc aggtagaaga gtaccaggag
     1321 cattacaaaa agttatgcca catatagatg gaatgtctaa tattatgtca aatagtggtg
     1381 gtgcttcaaa ttttaataat tcaatggcaa atgccagtgt tactaatata tttagtctta
     1441 taaaagaaat ggtacaatca caatttgcag ctttaggaga taagttttca aataatcctc
     1501 atatgaatat tggtatgatt gaaaaatcat atatgcaaag tttagatgga gctaaaggtg
     1561 caatagaaac agccttccaa aaaactatga atacagggta cactaaatta ttcgtaacat
     1621 gtgctattat agctttaata gggctaatat taacagctat gttaaataat aatttaataa
     1681 caatgaaaaa tagaagatta gaaaagaagg aaaaaaacta attgataaaa aatgtttttt
     1741 gagttatgat aaataaaaac aaagaatttt aagatattgc taggggtgct gtaaaaggct
     1801 gagagggata ataagtccta accctaataa cctgatttgg ttaataccag cgtagggaag
     1861 tatattttaa gttgtgattt ttcatattag aaaatattat tatgattaat tagagaatat
     1921 atttttaaag gtttagcaat attgctaaac ctttttttgt tggtataaaa gtcttaagaa
     1981 tatagaaaag aggtgaatag atgattgtta atggtaagaa agtagaaaaa ggtgaactta
     2041 atacagttta tgatttacta aaaagtttaa atgtaaatag taagagagtt gttgtagaac
     2101 taaacaaaga gatagttaat tcctgtaatt ataatgaaat cttacttaat catgaagatg
     2161 taattgagat tattgccttt gttggtggag ggtgattaag aatcatttaa gaagtttaag
     2221 ataacaaaag gtgagtgaaa ataaaagata aaaaagataa gagggacctt tatgaatata
     2281 aagattaatg aaaagtggag agatgtaaaa gaaaattgca cattatattc tttaaaaaat
     2341 gaagaatttc ctgattctca tgttatagtt ttaaatggat ttccacttgt agaagataag
     2401 gatcttaagg aaggagacag ggttgtcttt ataaaaaagg gaattatgcc taataaagag
     2461 gagttagaag aactaatggt atctaggcat acaccaggta ttcatgaaaa actaaaaaaa
     2521 attaaggttt taatagctgg atgtggagga cttggatcta atattgctat ttctttagga
     2581 agaataggga ttggaagtat aagtttagta gattttgata tagtagaacc ttctaattta
     2641 aataggcagc agtattatat tgaggatatt ggagaattta aagtaattgc tttaaaaaga
     2701 aacttaatta gaataaatcc ttttataaaa atagaagctt taaatcttaa gctttctgaa
     2761 gataatatgg gtattttaca aggagctgac ataataatag aagcctttga taatccagat
     2821 tataaagcct tattagctaa ttatgtttta agtaatatga aggacaaatt tttgatttcc
     2881 tcttcaggaa tggctggatt ttatgattca aatttaataa aaacaaagca aataacggat
     2941 aaattttata tttgtggaga tttagtaaat gaagctaagg aaggctgtgg gttaatggct
     3001 cctagagtgg ctatatgtgc aaatcacatg gctaatttag ttatagatat agctgttaat
     3061 aaaatagaat tttaaaaaga agggctagta aaaatagagt ggattttaag tggtttttag
     3121 atagagttta tatataaaaa ataaaatact agtctaaaat atgaagaact aagaagagta
     3181 gtgagtagaa aaggggaatt ttttatggac aagttaataa taggtggagt agaaataaaa
     3241 aatagacttt ttgttggaag tggtaaatat ccttcaaatg aaataataaa ggatgtttta
     3301 gagggaagtg gctctcaagt tataacttta gccttaagaa gggtagattt agataataag
     3361 gaagaggata ttcttcaaaa tatacctaag gatgtaatat tacttcctaa tacttctggg
     3421 gcaactaatg cagaggaggc tattagaata gcaaggatag caagagctat gggatgtgga
     3481 aactggataa agatagaagt tatatcagat tctaagtatt tgcttccaga taatgaagaa
     3541 accataaagg caactaaggt attagctgat gaaggcttta ttgtccttcc ttatatgtgt
     3601 ccagatcttt atgcaggaag aaggttaata gaagctggag cggcagctgt aatgccactt
     3661 ggtgcaccaa taggaagtaa tagagggtta aagactaaag aattgattca aataatgata
     3721 gatgaattag atattcctat tatagttgac gcaggtatag gaaagccatc acaggctatg
     3781 gaagccatgg aaatgggagc tgcagcttgt ttagttaata ctgccatagc atcctcagaa
     3841 gatcctataa atatggctag agcttttaaa atggcagtag aaggtggcag attagcttat
     3901 gaagctaaaa tgggaagaga atcaaagttt ggaaatgctt catctccact tactggattt
     3961 ttagattagg agatgattta attgagtttt tatgatgtag tagaaaaata tagagatttt
     4021 gatttttatg gatattttga ttctgtaaaa aaggaagatg tgttaagaag tatttatgaa
     4081 agaaataaga ggccagagga tttacttaat ttaatatctc ctatgggaga attagtttta
     4141 gaagaaatgg ctcaagaagc aagaaatctc tccttaaaat attttggaag aacaatatta
     4201 ttatatacac ctatgtatat ctcgaattat tgtgtaaata agtgttcata ttgtgggtat
     4261 aatgtagaaa ataaaatatg taggaaaaaa ttaaatcaag aagaaataga aaaagagggg
     4321 gaagctattt caaaggaggg atttaaacat attctaatat taacaggaga aagtgaatat
     4381 catactccag tagagtatat agaaaagagt attaaaactt tgaaagggaa atttccttca
     4441 ataaccattg aaatataccc aatgacagaa gagggatata aaaaagtggt agaagcaggt
     4501 gctgaagggc ttactgtata tcaagagacc tatgatgaaa aggtatatga tagggttcac
     4561 gtggctggtc caaagaaaaa ttataaattc agattagaag ctccagagag aggagcagaa
     4621 gctggaatga gaagcataag tataggagcc ttattaggat tagctgattt tagaatagat
     4681 gccttcttta cagcaatgca tggaaaatat ttaagggata agtatcctca tatagatata
     4741 agttattcag ttccaagaat aagacactgc gaaggagggc ttaaaaagtt aaatgaagtt
     4801 tatgataggg aactagttca aatactttta gcctatagac tatttgatcc ccaaggagga
     4861 ataaatatat ctactagaga aggaaaggat tttagaagaa atttaattcc cttaggagtg
     4921 agtaaaatca gtgctggagt ttcaactgag gttggaggcc attctttaaa agaaaaaggt
     4981 acaagtcaat ttgatataaa tgatgaaagt tctgtaagtg aagttaagga attaataaaa
     5041 agtgaaggtt atcaacctat atttaaggat tggcatagat tttaaaataa ttttttatta
     5101 aaatggtgat ttttatgttt ttaataacca atagaaagtt agtaaatagg gaaaaatatt
     5161 ttaataccat agaggaagca ggtaaatatg gtgttaaaaa tataatttta agagaaaaag
     5221 atttaagcac agagaaattg atagaggttt atataaaaat aaaagagtta gttccagagg
     5281 aaacaaatat aataataaat tccaatattg aagcagcaaa gattttgaaa gagaagttta
     5341 ttcatttaag ctttaaggat tttaagaaga atttagaaga agttaaatcc ttagaggttg
     5401 gagtatctgt tcactctatc ttagaggcca ttgaagctga tagattaggg gcaagctata
     5461 tattagtatc tcctattttt gaaacgcaat gtaagaagga cgtaactcct aagggaatta
     5521 attttataaa agagattaag gaaaaggtaa attgtaaggt catagcctta ggcggaataa
     5581 atgagcttaa ctttaaagaa gtattaggag ctggcgctga tgattttgca tgtatgtcac
     5641 ttttatttat gagtaacaat ataaaggaat gcttagatac ttttaaatct ctttaaacat
     5701 atctagattt atatgaatga aaatgtcaat tatatgatta ttaatagaat tatggtataa
     5761 tttatattaa ttaataatta caatttgtta ttaaaggaga agtgattatg tcatttgaaa
     5821 gaatagatat ttcagataga agtgaacaag aaggaagaag ttgtgtaatc ttatataact
     5881 ttgaaaataa ggaattaaag aaaatacaaa actttgctag aataatggga ataaaagatc
     5941 aaatagtttt aaactctaaa aatggagatg aaactataaa gaatatatta gaggataaaa
     6001 ttgaaaaaac agaaggcgaa ggagttaaag aaagagcaat aatatttaat gccatagctc
     6061 ctggaaaagt tcatatgttt ttagaaaact taaaaaaaat aggcatacaa aggcctttag
     6121 ttgcaatggt tacagaaaca tctattaatt ggactattga cgttttagtt aaaaatttaa
     6181 tagctgaaag aagagcatta agatcaggag atatgactac tcacgaaaat aaattttaga
     6241 aaaattata
//
LOCUS       DAEVGI010000143         6248 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEVGI010000143 DAEVGI010000000
VERSION     DAEVGI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.143"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            33..650
                     /locus_tag="K8M77_002129"
     CDS             33..650
                     /locus_tag="K8M77_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086595.1"
                     /translation="MNFFNKNEEENSSIEEKKLVEEKNSVGEKSAVEVTLNKEGNVEN
                     VTFDKSEAVAYGVDLEKELEDEKNNEVDEQGELLNPIPTPVKQGENMLDYVVTLSDEQ
                     KIKLNEFIQANDFSAVSRLLSEHKRIISTPKELFNIVLNFNDSKIIPFKSAMVLGLDE
                     IDAFNVLARQYPILKELERNKISIKRINRVQQSQIILVDGEMKLS"
     gene            1374..2357
                     /locus_tag="K8M77_002130"
     CDS             1374..2357
                     /locus_tag="K8M77_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 family protein"
                     /protein_id="HBI7086596.1"
                     /translation="MISERVDLLINNGEILDEIEGTSERNIDRKYSALNLTLRNEIAN
                     IDRIEKAIKLIKENTSVFSEYRRRNLLNLAVNISLEEDMDKALSEIENIYINLKSEFS
                     QSRYLMLAAEEIFFSRNFINVEEVIMNTKSAYKYMKKNHRFETKREDLCSAAMIAMTS
                     KNLKETFDEINECYDLLTECGFSKNNDLELLSNMLSIINMPVDIKCAQVRDLATNLKE
                     NKVEFKKSYLPILGIAAFVTDDYNKLSKNILDVSETLKENEGFRSVTVDEKARNIMAL
                     ILVVKEYLDNLKDDSKLKLIKKSLDKSLEDVFAIASSGSVTIEEIDITVTQ"
     gene            complement(2495..3313)
                     /locus_tag="K8M77_002131"
     CDS             complement(2495..3313)
                     /locus_tag="K8M77_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBI7086597.1"
                     /translation="MMNVDTSKCIGCTLCMQDCIVSDIEMVDGKAHIKNESCIECGHC
                     IAICPKEAVSDSDYDMSKIQEYKKESFDIDSDRLLNFIKFRRSTRLFQNRDVEDEKLE
                     KIIEAGRFTQTGSNMQNVSYVVVKDKIQELRKIVLETLKSLGENLLNKETTPENIKRY
                     CYMWIKMYNDFLEDPNGEDKLFFKAPALIIVKSESTVNASLAASNMELMTNALGLGTY
                     FSGFLQRASEVNPKINEFLGLKENESLVNCMVIGYPKVKYKRTVPRKEAKITWM"
     gene            3611..3937
                     /locus_tag="K8M77_002132"
     CDS             3611..3937
                     /locus_tag="K8M77_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086598.1"
                     /translation="MKKHNKRKINEKHIEHALKVLSGKWRLKVLWEVYNYESIRFNQL
                     QKNVEGISSLMLTRVLKDLIDNNLVIRTQFNEVPPHVEYSLTDLGRSLCPIIRLLEDF
                     GKKTLE"
     gene            4191..6113
                     /locus_tag="K8M77_002133"
     CDS             4191..6113
                     /locus_tag="K8M77_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7086599.1"
                     /translation="MEVNSIGKTLKKALPPTILSMVLLLVNLKFFGLSNIIIAIYMTL
                     TFIRMRTYLIIENNIFKPLFIQLAIGVLASVASMGGLLEVLINFFGIIILVYLLTDEY
                     NPDSYFPYLMAFVLLQMFPVKFDQISNRLLGIFVSYIIVYLALMIVSPKGEDNKIQEL
                     IKSGFENIHSQFKNLYYDDIDKVKSEQIELFDICRELNRFVYSGGRRKYYQIMIAFQH
                     INNIIHDLKSSKEIIEENKKEFKRFYKLFKYLENNFKDYKLCAERLEEFEKEFKFHNK
                     NLTFYTSLVLRYLSESIEHLNYHRFNLRKFLNFKNNKDYFQVYSKYNLKLNEFKLRFA
                     IRMSVIVTLAFFVIRRFSLPKGYWLPMTVFILALPFYEDSKARVYARFRGTILGVIVA
                     FLLFSVFKGQEMHFVIILVTTFFMYAFKDYATMSIYVTCYALAITTISMSDGEAVILR
                     LLYTGIAAIVVLFANKFILPNKNHVELINMVKKLIDLDKVMITKAREALEKDFDEMEL
                     RRIIYSSYLISGRIQMHTNPSDEKEDKEIKKFMLSNSEFTTSIINYAVILSNSNKGAL
                     DYEYINEGISLIEEKLRSFKEEFFYNPNYIGSFCKNIQLINHEDNYKNYCLIKCVDRV
                     YNLEKNLNILKRTMTN"
BASE COUNT         2479 a          606 c          915 g         2248 t
ORIGIN      
        1 tggataaaaa ttagaattag gaggaattta atatgaattt ttttaataaa aatgaagaag
       61 aaaatagttc tatagaagaa aaaaaacttg tagaagagaa aaattctgta ggagaaaaga
      121 gtgctgtaga agttacttta aataaagagg gtaatgtaga aaatgttact tttgacaaaa
      181 gtgaagctgt agcatatggt gttgatttag aaaaagaatt agaagatgaa aaaaataatg
      241 aagtagatga acaaggagaa cttttaaatc caatacctac accagttaag caaggtgaaa
      301 atatgttaga ctatgttgtt actctttcag atgaacaaaa aattaagtta aatgagttta
      361 tacaagctaa tgatttttca gcagtaagta gattgctttc agagcataaa agaataattt
      421 caactcctaa agagttattt aacatagttt taaactttaa tgatagcaaa ataattcctt
      481 ttaaatctgc tatggtacta ggattagatg aaatagatgc atttaatgtt ttagcaagac
      541 aatatcctat attaaaagaa cttgaaagaa ataaaatatc cataaagaga attaataggg
      601 ttcaacaatc acaaattatt cttgtagatg gagaaatgaa attatcttaa gttaatatta
      661 gaaggttaaa taatctttta ttaagatatt agattatttt taatagacaa gctttaaagg
      721 tttaaaaaga gattttatga ttagagttaa atcataaaat ctcttttaac ttatattaaa
      781 taatataaga ttttaatatt tagtttctaa atatgttttt tattttggca tgaaatgata
      841 atcttaaaga ttactcctag tataaaaaag ccaaaaaata tacttaaaat acttaatgtg
      901 tatatgtgag ttgataacaa taagtcaaaa aatagaccca tgaataatcc aaatataaca
      961 taggctaaaa taaaaatttt cttttgttta taagtagaat ttctcataaa acaacacccc
     1021 tttaatcccc ttttatactt tcattataca ccaaaatata gaataatagg ttatgtatgg
     1081 aagttgagta ttaaatatat aagtatttaa atatttaata cttttagtta taagaaagtc
     1141 attttaatgt gttttatttt gataattatg taaaatttat ggtgtttttt taaggagtat
     1201 aataggctat taaataatgc cttttgaaaa taagagtata attttattaa taaagttatg
     1261 taagttaatt tctgtaatac agtacataaa ttcatactaa atattagttg tattacagta
     1321 gttaatttgc acttatttat aattattttt ttgaaaaaga aaggatgatt attatgatta
     1381 gcgaaagggt agatttatta attaataatg gtgagatttt agatgaaatt gaagggactt
     1441 ctgagagaaa tattgataga aaatatagtg ccttaaacct aacattaaga aatgaaattg
     1501 ctaacataga tagaatagaa aaagcaataa aacttataaa agaaaatact tcagtttttt
     1561 cagaatatag aagaagaaat ttattgaact tagctgtaaa tatatcctta gaagaggata
     1621 tggataaagc tttaagtgaa atagaaaaca tttatattaa tcttaaaagt gaattttcac
     1681 aaagtagata tttaatgtta gcagcagaag aaatattctt ctcaagaaat tttattaatg
     1741 ttgaagaagt gataatgaat acaaagagtg cttacaagta tatgaagaaa aatcatagat
     1801 ttgagactaa gagagaagat ctttgttcag cagcaatgat tgcaatgact tcaaaaaatc
     1861 ttaaagaaac ctttgatgaa attaatgaat gttatgatct tttaacagag tgtggctttt
     1921 caaaaaataa tgatttagaa ttattaagca atatgttatc aataataaat atgcctgtcg
     1981 atataaaatg tgcacaagta agagatttag caacaaattt aaaagaaaat aaggttgagt
     2041 ttaagaaatc ttatcttcca atactaggaa tagcagcctt tgtaactgat gactacaata
     2101 agttaagtaa aaatatttta gatgtttcag agactttaaa agaaaatgaa ggatttagat
     2161 cagttactgt ggatgaaaaa gctagaaata ttatggcttt aattttagta gttaaggaat
     2221 atttagataa tttaaaggat gattctaagc taaagcttat aaaaaaatct ttagacaaat
     2281 ccttagagga tgtatttgca attgcttcat ctggtagtgt tactattgaa gaaattgata
     2341 taaccgttac acaataatta gcttaaaatt tattatataa ttaatttata aagtaaaaag
     2401 tatttttaac aaaatggtat ggaaatttgt atttgagata aagggcgtta tcaaagtatg
     2461 aagttatttt gatacatgcc ctttatttgt ataattacat ccaagttatt ttagcttctt
     2521 ttcttggtac agttctttta tatttaactt tagggtaacc aataaccata cagtttacta
     2581 aggattcatt ctcttttaag cctaagaatt cattgatttt agggtttact tcagaggctc
     2641 tttgtaagaa tccactaaaa tatgttccaa gtcctaaagc attagtcata agctccatat
     2701 tagatgctgc aagagaagca tttacagtag attctgattt aacaattata agtgcaggag
     2761 ctttaaaaaa tagtttatct tcaccatttg gatcttctaa gaaatcatta tacattttaa
     2821 tccacatata acaatatctc tttatatttt caggagtagt ttctttattt aataaatttt
     2881 ctcctagaga ttttaatgtt tctagaacta tttttctaag ttcttgaatc ttatctttta
     2941 caacaacata agaaacattt tgcatattac tacctgtttg agtaaatctt cctgcttcta
     3001 ttattttttc aagtttttca tcttcaacat ctctgttttg gaaaagtctt gtacttcttc
     3061 tgaatttaat aaaatttaaa agtctatctg aatctatatc aaaactttct ttcttatatt
     3121 cttgaatttt actcatatca taatctgaat ctgaaacagc ttccttagga caaatagcaa
     3181 tacagtgtcc acattctatg cagctttcat tttttatatg ggccttacca tctaccattt
     3241 ctatatcact aactatacag tcttgcatac aaagagtaca gcctatacat ttactagtat
     3301 ctacattcat cataataaat caacctacct atctaataat ttatatatct aaataaaatt
     3361 atagtaatta tgaattttta agacaagtac gcaatattta tttagttact tacaaaaagt
     3421 taagtattga agttaagttt atttagttta atattatata atttataatg gatttaaagt
     3481 aaataagact ttagaaaagt gaaatatatt tgtataaatg ctttatttaa gaagaagtaa
     3541 aatagactat taagaaatat tattatgtga tatcattaag cagtaaaata aattttagga
     3601 ccggtgttat atgaaaaaac ataataaaag gaagattaat gaaaagcata tagaacacgc
     3661 tttaaaggtt ttaagtggta aatggagatt aaaagtttta tgggaagttt ataattatga
     3721 aagcataagg tttaatcaac tacagaaaaa cgttgaagga atatctagtc ttatgctaac
     3781 aagagtttta aaagatctta tagacaataa tcttgttata agaactcaat ttaatgaggt
     3841 gcctccccat gttgagtact ctttaacaga tttagggaga agtctatgtc ctataataag
     3901 gcttttagag gatttcggca aaaagacatt agaataaaaa tataaaaaat caaaatttac
     3961 ttaaaataca gtttaaaatt aaagtttaaa agaggagttt taatatccaa attaggtagg
     4021 aatattaaaa cttcttattt ttttatttaa ttttcaatct ttaaaattta cttatgaaat
     4081 ataatttgaa taggaggtaa aatgtgaaca aattgtaact aaatgtggta caatttttat
     4141 agaagtatat ttaaaataag aaaataagag tttgaaagaa ggggaaagcc ttggaagtaa
     4201 atagtatagg aaaaacttta aaaaaggcat taccaccaac tattttatct atggttttac
     4261 ttttagtaaa tttaaagttt tttggattaa gtaatataat tattgcaatt tatatgactt
     4321 taacatttat aagaatgagg acatatctaa taattgaaaa taatattttt aagcctttat
     4381 ttatacaatt agccataggg gtattagctt ctgttgcaag tatgggaggg cttttagaag
     4441 tattaataaa tttttttgga ataataatat tggtttattt acttacagat gagtataatc
     4501 cagatagtta tttcccttac ttaatggcct ttgttcttct tcaaatgttt ccagtaaaat
     4561 ttgatcaaat ttctaatagg cttttaggaa tttttgtatc atacataata gtttatttag
     4621 ctttaatgat tgtatcacct aagggagaag ataataaaat tcaagaacta ataaaaagtg
     4681 gatttgaaaa tattcattct caatttaaaa atctttatta tgatgatata gataaagtaa
     4741 aaagtgaaca gatagagctt tttgatattt gtagggagct taatagattt gtgtactcag
     4801 gtggaagaag aaagtactat caaattatga ttgcttttca acatataaat aatattatac
     4861 atgatctaaa aagttcaaag gaaataattg aggaaaataa aaaggagttt aaaagattct
     4921 ataagttatt taaatatctt gaaaataatt ttaaagatta taagttatgt gctgaaaggt
     4981 tagaggaatt tgaaaaagaa tttaaatttc acaataaaaa tctcactttt tatacaagtt
     5041 tagttttaag atatttatca gaatctatag aacatttgaa ctatcataga tttaacttaa
     5101 ggaagttttt aaactttaaa aataacaagg attattttca agtttatagt aaatataacc
     5161 ttaaacttaa tgaatttaaa cttagatttg ctataagaat gtcagttata gtaactttag
     5221 cttttttcgt aattagaaga ttttccttac caaagggata ttggttacca atgacagtct
     5281 ttattttagc tcttcctttt tatgaagata gcaaagcgag agtttacgct agatttagag
     5341 gaactatttt aggagttata gtagcatttt tactattttc tgtttttaaa ggacaagaga
     5401 tgcattttgt tataatttta gttacaacat tttttatgta tgcttttaaa gattatgcaa
     5461 caatgagtat atatgtaact tgttatgctt tggccataac taccatatct atgagtgatg
     5521 gggaagcggt tatattaaga ctattatata caggaattgc tgctatagta gtattatttg
     5581 ctaataaatt tattcttcct aataaaaatc atgttgaact tataaatatg gtaaaaaagt
     5641 taattgattt agataaggtt atgatcacta aagcaagaga agcactagaa aaagattttg
     5701 atgaaatgga attaagaaga attatttatt cttcatattt aattagtggg agaatacaaa
     5761 tgcatacaaa tcctagtgat gaaaaagaag ataaagaaat taaaaagttt atgctttcaa
     5821 atagtgaatt tacaacttca attataaact atgcagttat tttgagtaat tcaaataaag
     5881 gagccttaga ttatgagtac ataaatgagg gtataagttt gattgaagaa aaattaagga
     5941 gttttaagga agaatttttt tataatccta attatatagg aagcttttgt aaaaacattc
     6001 aattaataaa ccatgaggat aattataaga attattgttt aattaagtgt gtagatagag
     6061 tttataatct tgaaaagaat ttaaatatat taaagagaac aatgacaaat taagataata
     6121 attatgaggg tgtatttagt attgatagaa ctttaaatga aagtatcact acattataat
     6181 atatagatta agaaatttaa aattaacata attaaaaatt ttttgaaagt tacacataaa
     6241 tgtgtagc
//
LOCUS       DAEVGI010000144         6241 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEVGI010000144 DAEVGI010000000
VERSION     DAEVGI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.144"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            116..2131
                     /locus_tag="K8M77_002134"
     CDS             116..2131
                     /locus_tag="K8M77_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBI7086600.1"
                     /translation="MNKKYFKELLGNKKTLKNLGINVFIWITLSFLLVFLGESFHRGS
                     IENGKDFLDNQITVFLLNYLIFLMITSIAFFFKKTRMVYGIISTILMGFYMASGVIIG
                     FRGTPLIWADMFSFKEGLAIAGNYLNLNILKYTVIALVIIIAILVFLWFSERYKSRNK
                     VINPYGFIIFPLSILAVGAFYGNAKGSIEVYRWNLPVSYERNGFMYSFLDTAAGFKVK
                     EPSDYNKASIEKIKNDIIEESQLASNDTKMASAMPAKFPNIIIVQLESFMDLHRINGL
                     TFKEDPIPTFRKISSESTKGFLKVPTYGGGTVRSEFEVLTGLSTDYLPVGEIPNNNIL
                     KKQPVESLAYILHDYGYGTNVIHNYEGNFYNRDTVYPNLGFDKYISMEYMDKPNNQSW
                     QYPEDVLNIEPIEDIISNNEKPQFIYNVTVESHGGYSSSDFENYTVDGNLDQEEKNEL
                     QCYIDKLRGVDEYIKELLDYVESSGEPTVIAMFGDHLPSLKIINDDESVLKDGNKYLA
                     DFFIWDNIGLPKENINMEAEEFTTYILEKLNMVAGVMPTFHNAYKDDEHYKEDFELLQ
                     YDMLFGNKYILNENKNKYEKTNMKMGLKEITLNNYDIKDDILTVTGDNFNYKSKIIIN
                     GKIKETNFIDENTLTTTEIPSNIKSISVGQIGKYDKILSSSNSLEIK"
     gene            2242..2511
                     /locus_tag="K8M77_002135"
     CDS             2242..2511
                     /locus_tag="K8M77_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086601.1"
                     /translation="MKIIKKDGRIEEFKIEKLSTSLSNAASDLNFTLNKSDINIIAKD
                     VEETLKKIRKDNGETSSYEVIGVVIDTLKNEKFSDVLRAYVKYRK"
     gene            2620..3462
                     /locus_tag="K8M77_002136"
     CDS             2620..3462
                     /locus_tag="K8M77_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086602.1"
                     /translation="MIKKTVVAIGLVFSLSFLVGCGNKSLVEQGKVAIKQGNYEEATA
                     IFKAASEEDSKDKEEAESLYDLTYNYVISNKAYNEFKLDVALDSLKKVEENKNKELMK
                     EDITSLKEEISKDKSVLDDFYSSMEKVNDLENEGKISEARDLLSKSLKNISYLGENVK
                     ELIASGNSKLKNLDEKLDKKEIVKEDNSKEEKTDSSENNKELANKALETVKSFGVVKK
                     DQYLELKSDEVYDLGDGSYGFIVNKYYYPEGKPEPAFICQYLVDKESWKVKEVKEGII
                     KELN"
     gene            complement(3518..4054)
                     /gene="lepB"
                     /locus_tag="K8M77_002137"
     CDS             complement(3518..4054)
                     /gene="lepB"
                     /locus_tag="K8M77_002137"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI7086603.1"
                     /translation="MKCFNYILDLPDSFFKIIITILISLILILGFLLNNIFIAVVDGS
                     SMEDTIHHGDVLIINKKSYSTTSPKRYDIVNIYAHYKYDNFLVKRIIGLPGDTIEINN
                     SEVYINGDKIYESYIKEEMNIPYYLKLNIPKNKFFVMGDNRNISLDSRYFGLVQGTDI
                     QGKAIFKYCYKNHKFKFF"
     gene            4229..6196
                     /gene="pulA"
                     /locus_tag="K8M77_002138"
     CDS             4229..6196
                     /gene="pulA"
                     /locus_tag="K8M77_002138"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HBI7086604.1"
                     /translation="MRRNFNSKEFNDKYYYDGELGAIYNKEKTIFRVWSPEAKSLELL
                     LYRDGNTESLIERDLLHKKSNGLWELVKKGDLNGVYYKYHIVGEDFEHDFVDPYCKAL
                     GVNGYRAMVIDLKKTNPKGWENEKKPSLENPLDSILYEMHLRDFSIDESSGVSLENRG
                     KFLGIVEENTKVPGTEVKTTLDHLKELGITHVHLLPSFDFGTVDEERLDEEQYNWGYD
                     PVNYNVPEGSYSKNPYKGEVRIKEFKEMVLKLHKAGIRVVMDVVYNHTYSGENSNLNL
                     SYPGYYHRQDDFGNFSNGSGCGNELASERLMVRKYMVDSLKYWAREYHIDGFRFDLMA
                     LHDIETLKGIREELNKIDPSILIYGEGWNGGESPLPKEEACFKCNIEKFDKLQIAAFS
                     DDMRDGIKGHVSHLKDGGFVNGGEDFEESIKFGIVASTYHEGVDYNKVNYSDSPWANE
                     PYQTVNYCSAHDNNTLHDKLKIVCENASEEEIIEMNKLSAAIFLTSQGIPFIHSGEEL
                     LRTKINEKGEFIENSYNSNDFVNKIDWTRKVKYMDLFKYYKGLIELRKEYPLFRLESN
                     KEIREKISFIESKLGINEKGIVAYRLKDNNNEFIVIFNSNNNEVKINLPKGLWGVMVN
                     NKFSGKEIRDEARDFYDIIRKSAYVFKKISN"
BASE COUNT         2539 a          556 c         1100 g         2046 t
ORIGIN      
        1 ggagagttat atcaaaggaa agtagattac tattatagtt ttcaaaatcg gactcgtaga
       61 acagggaatt aaacaagatt tatagatatt tgtagatttt tggcaacggc ataagatgaa
      121 caaaaagtac tttaaagagc ttttaggaaa taaaaaaact ttgaagaatt tggggattaa
      181 cgtatttatt tggattactc tatcttttct attagtattt ttaggggagt catttcatag
      241 aggatctatt gaaaatggaa aggatttttt agataatcaa ataacagtat ttctattaaa
      301 ctatctaata tttttgatga ttacttcaat agcttttttc tttaagaaga ctagaatggt
      361 ttatggaata ataagcacta ttcttatggg attttatatg gcaagtggag taataatagg
      421 ctttagagga acaccactta tatgggcaga tatgttttct tttaaagaag gattagctat
      481 agcaggaaat tatttaaact taaatatttt aaaatatact gttatagcct tagtaattat
      541 aattgcaata ttagtatttt tatggtttag tgaaagatat aaaagtagaa ataaagtgat
      601 taatccatat ggctttataa ttttcccatt atctattttg gctgtaggag ctttttatgg
      661 aaatgcaaaa gggagtatag aagtatatag atggaatttg ccagtttcct atgagagaaa
      721 tgggtttatg tactcatttt tagatactgc ggcaggattt aaggttaaag agccaagtga
      781 ttacaataag gcatctattg agaaaataaa gaatgatata attgaagaat cacaactagc
      841 tagtaatgat actaaaatgg catctgctat gccagcaaaa tttcctaata taataattgt
      901 tcaattagaa tcatttatgg atttacatag aataaatgga ttaactttta aagaggaccc
      961 tataccgacc tttagaaaaa tatctagtga atcaacaaag ggctttttaa aggtacctac
     1021 ttatggaggt ggaactgtta gaagtgaatt tgaagtgcta actggattaa gtactgatta
     1081 cttaccagta ggtgaaatac ctaacaataa tatacttaaa aaacagccag ttgaaagttt
     1141 agcttatata ttacatgatt atggatatgg aacaaatgtt atacataatt acgaaggaaa
     1201 tttttataat agagacacag tgtatcctaa cttaggattt gataaatata tatctatgga
     1261 gtatatggac aagccgaata atcaatcttg gcaatatcca gaggatgttt taaacataga
     1321 acctatagaa gatataatat ctaataatga aaagcctcag tttatttata atgtaactgt
     1381 agaaagtcat ggaggatatt cttcttcaga ttttgaaaat tatacagtgg atggaaattt
     1441 agatcaagaa gaaaaaaatg aacttcaatg ttacatagat aaattaagag gtgttgatga
     1501 atacataaaa gaacttttag attatgtaga atcttcagga gagcctacag taatagctat
     1561 gtttggagat catctacctt cattaaagat aataaatgat gatgagagtg tattaaaaga
     1621 tggaaataaa tatttagctg acttctttat atgggataac attggcttac caaaggaaaa
     1681 tataaatatg gaagcagagg agtttacaac atatatatta gaaaagctta atatggtggc
     1741 aggagttatg cctactttcc ataatgcata caaggatgat gagcattaca aagaagattt
     1801 tgaacttctt caatatgaca tgctatttgg aaataaatat atcttaaatg aaaataaaaa
     1861 taaatatgaa aaaaccaata tgaagatggg attaaaagaa ataactttaa ataattatga
     1921 tataaaagat gatatattaa ctgtaactgg tgataatttt aattataaga gcaaaattat
     1981 tataaatgga aagattaaag aaactaattt tatagatgaa aatacattaa caactactga
     2041 aattccttca aacattaaaa gtatttcagt tggccaaata ggaaagtatg ataaaatact
     2101 aagtagtagc aattcattag aaataaaata gtacttttat ttattagatc ttagaataaa
     2161 tttagctttt aagaataaaa tgtaaatgtt atacttaatt taataattac taaattatac
     2221 taatttaaca ggaggatatt tatgaagata ataaaaaaag atggaagaat tgaagaattt
     2281 aaaattgaaa aattatcaac aagcttatct aatgcagcaa gtgatttgaa ttttacactt
     2341 aataaatcag atataaatat aattgcaaag gatgttgagg aaacattaaa aaaaataaga
     2401 aaagataatg gagaaacttc aagttatgaa gttattgggg ttgtaattga tacattaaaa
     2461 aatgagaagt ttagtgatgt tttaagagca tatgtaaaat atagaaaata attaaaaggg
     2521 gctgtatcaa atataacaaa taaaacttat tttgatacat gccttttatt tgtaaagagt
     2581 atattttatt aatggtaaat aagggagaga agttaaaata tgataaaaaa aacagttgta
     2641 gctataggtt tggtatttag tttaagtttt ttggttgggt gtggaaataa aagtttagta
     2701 gaacaaggta aagttgccat aaaacaagga aattatgaag aagctacagc aatttttaaa
     2761 gcggctagtg aagaggattc aaaggataaa gaagaagctg aaagcttata tgatttaaca
     2821 tataattatg taatatctaa taaagcatat aatgagttta agttagatgt ggctttagat
     2881 agtttaaaga aagtagaaga aaataaaaat aaagaattaa tgaaagaaga tattactagt
     2941 ttaaaagaag aaataagcaa agataagagt gtattagatg acttttactc aagtatggaa
     3001 aaggttaatg atttagaaaa tgaaggtaag atatcagaag caagagattt gctaagtaaa
     3061 tctcttaaaa atatttctta tttgggagaa aatgttaagg aactaattgc aagtggaaat
     3121 tctaaattga agaatttaga tgaaaagctt gataaaaagg aaatagttaa agaagataat
     3181 agtaaagaag aaaaaactga ttcttccgaa aataataaag aattagcaaa taaagcttta
     3241 gaaactgtaa aaagttttgg tgtagtaaaa aaagatcagt atttagagtt aaaatctgat
     3301 gaagtatatg acttaggtga tggaagttac ggttttatag taaataaata ctattatcca
     3361 gaaggaaagc cagagccagc atttatatgt cagtatttag tagataaaga atcgtggaaa
     3421 gttaaagagg ttaaagaggg tattattaaa gaattaaatt aaaaatttat gacaaactag
     3481 agtgatattt tattctctct agtttgttta atttaaatta aaagaattta aatttatgat
     3541 tcttatagca atatttaaag attgccttgc cttgaatatc agtaccttgt actagtccaa
     3601 aatatctaga atctaaactt atattcctat tatcacccat aacaaagaat ttatttttag
     3661 gtatatttaa ttttaaataa taaggaatat tcatttcttc ttttatatat gattcataaa
     3721 ttttatcacc atttatatat acttcagaat tatttatttc aattgtatca ccaggaagac
     3781 ctataattct tttaactaga aaattatcat atttataatg tgcataaata tttactatat
     3841 catacctttt aggagaagtg gtggaatagg attttttatt tataattaat acatcaccgt
     3901 gatgaatagt atcttccatt gaagagccat caacaacagc tatgaatata ttgtttaaga
     3961 ggaaacctaa aattaaaatt aaggatatta atatggttat tataatctta aaaaagctat
     4021 caggtagatc taaaatgtaa ttaaagcatt tcatgaatta ttacctcatg tatattaaga
     4081 atatattatt agattatttt ttatgtattc aaataagaac taataattaa ctttaagttg
     4141 aaatttaatt actactttaa tataattaag ttagttgtaa tttatataca attagttgga
     4201 aacgttaccg aagtggggag aggattttat gagaagaaat tttaattcta aagagtttaa
     4261 tgataagtat tattatgatg gagaacttgg agctatttat aataaagaga agactatttt
     4321 tagagtatgg tcaccagaag ctaagagttt agaattgtta ctttatagag atggaaatac
     4381 agagagttta atagaaagag atttgcttca taaaaaaagc aatggacttt gggaattagt
     4441 aaagaaggga gatttaaatg gagtttatta taagtaccat attgtaggag aagattttga
     4501 gcatgatttt gttgatcctt actgcaaagc cttaggagtt aatgggtata gagctatggt
     4561 tattgacttg aagaaaacta atcctaaggg atgggaaaat gaaaagaaac catcacttga
     4621 aaatccctta gattcaattt tatatgaaat gcatttaaga gatttttcaa ttgatgagag
     4681 ttcaggggtt tctttagaaa atagaggaaa gtttttagga atagttgagg aaaatacaaa
     4741 agttcctggt acagaagtaa aaacaacctt agatcattta aaggaattag gaataaccca
     4801 tgtacatctt ttaccttctt ttgattttgg aacagttgat gaggagaggt tagatgaaga
     4861 gcagtataat tggggatatg accctgtaaa ctataatgta ccagaaggat catattctaa
     4921 aaatccatat aaaggtgaag ttagaattaa agagtttaag gaaatggtac ttaaacttca
     4981 taaagcagga ataagagttg tcatggatgt tgtatataat catacttatt caggagaaaa
     5041 ctctaattta aatttatctt atccaggata ttatcataga caagatgatt ttggaaattt
     5101 ttctaatggt tcaggatgtg gtaatgaatt agcatcagaa agacttatgg taagaaaata
     5161 tatggttgat tcattaaaat attgggcaag agaatatcac atagatggtt ttagatttga
     5221 tttaatggca ttacatgaca ttgaaacctt aaagggaata agagaagaat taaataaaat
     5281 agatccttca atattaatat atggagaagg gtggaatgga ggggaatctc cactgcctaa
     5341 agaagaagct tgttttaaat gtaatataga gaaatttgac aaattacaaa tagcagcttt
     5401 tagtgatgat atgagagatg gaataaaagg acatgtatca catcttaaag atggaggctt
     5461 cgtaaatgga ggagaagact ttgaagagag tataaagttt ggaatagtag cttctactta
     5521 tcatgaaggt gtagattata ataaagtaaa ttattcagat tctccttggg caaatgaacc
     5581 ttatcagaca gtaaattatt gttctgccca tgataataat acattacatg ataaacttaa
     5641 aattgtatgt gaaaatgctt ctgaagaaga gataatagaa atgaataagt taagtgctgc
     5701 tattttctta acatctcaag gaataccatt tattcattca ggagaggagc ttttaagaac
     5761 taagattaat gaaaaaggag aatttattga aaatagttat aattctaatg actttgtaaa
     5821 taagattgac tggactagaa aagtaaagta tatggattta tttaaatact ataagggatt
     5881 aattgaatta agaaaggaat atcctttatt taggttagaa agtaacaaag aaataagaga
     5941 aaaaatttca tttatagaga gtaagttagg aataaatgaa aaaggtatag ttgcttatag
     6001 attaaaagat aataataatg aattcatagt aatttttaat agtaataata atgaagttaa
     6061 aataaacctt cctaagggcc tttggggagt tatggttaat aataaatttt caggaaaaga
     6121 aataagggat gaagctagag atttttatga tataataaga aagtcagcat atgtttttaa
     6181 aaaaataagt aattaaatat aattgtaaaa tttagagttt aataaaatga attttaatat
     6241 a
//
LOCUS       DAEVGI010000145         6054 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEVGI010000145 DAEVGI010000000
VERSION     DAEVGI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.145"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            127..654
                     /locus_tag="K8M77_002139"
     CDS             127..654
                     /locus_tag="K8M77_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086605.1"
                     /translation="MGVTTYKTKNGNFIIDSKKLNEKVTEVYKTYGFDLNLYEFEDEV
                     DKVFYLRYAVIKLKEELVGRKNKSEKQKLAENWYDMMSEKFGADWDKENAWCKYISEK
                     YILAEVTPNGKEVPEAYYDTKVEAIMEFLKMGACDNLKVFRGTVVCKDICGEEVLISL
                     RKKDDYVFPPFRKGF"
     gene            1132..5843
                     /locus_tag="K8M77_002140"
                     /pseudo
     CDS             1132..5843
                     /locus_tag="K8M77_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474547.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         2549 a          586 c         1160 g         1759 t
ORIGIN      
        1 aagaatttgt ctactactaa ataattatat tatacaacaa aatataaatt aataagatat
       61 aaagtttaat atcactaaag aaaatatctt ttaagaaata atatttctag attaaggaga
      121 aaaacaatgg gagttactac atataagaca aagaatggca attttataat tgattctaaa
      181 aaactaaatg aaaaagttac tgaggtttat aaaacttatg gatttgatct taatttatat
      241 gaatttgaag atgaagtaga taaggttttt tatcttagat atgcagttat aaaacttaag
      301 gaagaattag ttggaagaaa gaataagtct gaaaagcaaa aactagctga aaattggtat
      361 gatatgatgt cagaaaaatt tggtgctgac tgggataaag aaaatgcttg gtgtaaatat
      421 atttcagaga aatatatatt agcagaagtt actcctaatg gtaaagaagt tccagaagct
      481 tattatgata caaaggtaga agctattatg gagttcttaa aaatgggagc ttgtgataat
      541 cttaaggttt ttagaggtac agtggtatgc aaagatatct gtggagaaga agtattaatt
      601 tcacttagaa aaaaagatga ttatgtattc cctccatttc gtaagggatt ttaaaaatta
      661 taaagagaga taagtttaaa ataagctcat taggtgtaat gaaatattat acctaatgag
      721 tttattttta ttactataat taacgttatc aatgatttat taataaacta ttaataatgt
      781 taattataat ttatgaaaca gcaaaattaa tattttttaa aaaaattaat atataagtag
      841 gtgagggaat caaagtaata ctgcctttta aagggaatta atagaattta tttacaaaaa
      901 gttaataaaa agaacacttt caaaaaatta accaataatg tataatctat taaaatataa
      961 ataaagtgtt aagagaaatt aaaagattaa ggaattaatg taataattaa aattcattag
     1021 tttctatata aagcttaaaa tttttttata atataaatat ttggtagggg caactaagta
     1081 tttattataa aaatttttat aaataacata ataaaaaggg ggacaaattt aatgaaggaa
     1141 cagaaaaaac agatgaagag gtttttatcc tcaacgctca atggtttggt ggtattggct
     1201 tttataatgc ctagtagtgt aggaactaat gtaatggcag aggaaattca aaatgggaca
     1261 agccatacag taagaaattt agagaatatt gctagggatg aactttattt taagtatcaa
     1321 aatccaaatg aagtagtaag agttatagtt gaacttgaaa agccagcagc tatagaggaa
     1381 gctaaggctg aaggtgagaa aaaaccatct gaagcaaaaa ttcaagaagt aaaagaagaa
     1441 caaaaagatg ctaaggatga agcagaagaa attacaggag aaaagataaa taaaagcttt
     1501 ggaaccttaa taaatggatt cagtatcgat acaaaagtaa aagatataga ggaattaaag
     1561 aaaatcgatg gtgtaaaaag cgtaaaagtt gtaaagactt attatccagc tatgaattct
     1621 gctaaagatt taacacaggc agtagaaact tggaaagagt taggcttaaa aggtgaagga
     1681 atggttgttt ctattataga ttcaggaata gatccaaatc ataaagatat gaaaataaca
     1741 gattcatcaa aagctaagct taaaaaagaa aatttaaaag atggaccagg aaaatatttt
     1801 acagaaaaaa ttccatatgg atataatttt gctgatgaaa atgaaaatat tatagataca
     1861 catccaaaag tagatatgca tggaatgcat gtagcaggaa tagttgctgc caatggaagt
     1921 gatgaagggt tgctaaaaat gaggcaataa aaggagtagc accagaagca caattactag
     1981 ctatgaaagt tttttcaaat aatcctaata gacaaggggc agctgaggat gatatagtag
     2041 ctgccattga agagtctgtt aatcaaggag cagacataat aaatatgagt ttaggatctt
     2101 ctgctggatt ccaaaaagaa gatgatccag aacaaatagc cgttaaaaag gctgtggatg
     2161 ctggggtagt cgttgttgtg gctgctggaa attcacaata ttcaacggct ccatacaagg
     2221 ttccggatat aaaggatact ggtttagtag gagctcctgg aactgcaaag gatgcactta
     2281 cagtagcaaa ctatcataat agtaagatgc tattaccaac aataagcttt gaagataatg
     2341 gtgaagcagt taatatacca tttatgttat caggagaaga aaatagtctt aatttagata
     2401 aagactttga tttagtagat tgtggacttg gaaaggtaca agattttaaa ggaaaagatt
     2461 taaagggaaa agttgcctta ataaaaagag gggaaattac ttttatagat aaaaatttaa
     2521 atgcacaggc agctggtgct gaagggataa taatatacaa tggagatggt gatgagtcat
     2581 ttataaatat ggcaacagat ccaaaggtta aaattccatc agtatttgtt aaaaactcag
     2641 atggggaaaa atttaaaaat gctattaata agaatttaaa gataaagttt acaaacaata
     2701 aaatattagt tgcaagtagt gatgctggtg attttgttga atcatcatca tggggaccta
     2761 ctccaagctt agactttaaa ccacaaatat ctgcaccagg tggaaatata tattcaacta
     2821 taaatgataa taaatatggt attaagactg gtacatcaat ggcagcgcca catgttgctg
     2881 gaggagaaac attaatagtt gaagggctta aaaaggaaaa tccaaatctt aagggaagag
     2941 atttagtaga attagcaaaa aatacagcaa taagtacttc taagatagag atggataaaa
     3001 ataatcctaa gataccttat tcacctagaa gacaaggagc tggtcttatg caaatagagg
     3061 aagctcttaa aaataaggtt gtagtattag atgaaaataa taattctact gtggcattaa
     3121 agcaaatagg aaatgagaaa gaatttacat taacattaaa aaattatgga gataaagaag
     3181 ctgagtatga tgttgaaaat ttaggtggag ttttaacaga aactagtgat actttaaaga
     3241 ctatgtctca tgatgtaagg attgaggggg caaatcttaa gtttgataaa aataaagtta
     3301 ttgttccagc taagggtaca gaaactttaa aagtgaaatt aacaatacct aaagccgttt
     3361 cagaggatag atttgttgaa ggatttatta aacttacagg aaaagatgtt ccatcattat
     3421 cagttccttt cataggatat tatggagact ggggaaaaga tcaaataata gaagctatga
     3481 attgggatag taaaaatcaa aagttcatag ttccatcaga agtattaaca aatttaaatg
     3541 gagcaattgg gtacaagcta ggtttaggag caaaggatga aaagggaaat cttaaagtag
     3601 atcctagtaa aatagcaata tctccagatg gaaatggaaa tggtgatatc atagctccat
     3661 atttatatta tttaagaaat gctaaggtaa ctgaattaga gttattagat aaagataaaa
     3721 aatccttagg agttatagga catgaaaatt atataagaaa agaggaatat agtgaaccaa
     3781 gtggaagtgg aaaagctcca aacttatttg agaacttaac ttgggatgga aagttatata
     3841 accaaagtac aggagaaaag gaagttgtac cagaaggaca atattattta aatataaaat
     3901 caaaagttga ttatgataat gctaaatatc aagaggtagt tgttccagta caagttgacc
     3961 ttactgcacc taatattgaa ataacttcag gagacaaagt attaggtaat aaggacgata
     4021 atgaagtaga ttataaatta gaatggactg ctaaggataa tgtttctatt ataccagata
     4081 tagctacagt atatgtaaat ggtaaaagtg taagcgctaa tataagtgaa aataatggca
     4141 cttatagttg tgatataaag ttaaaaaaca atgctttaaa tgaagttaag gtagctatga
     4201 atgatacagc acttaattta gctgaagtat ctaagaatat aaaggttgaa tcttcagatc
     4261 cattaataaa atttgaaggt aactttggaa ctgctacttt aagtgttgat aattctttag
     4321 aatatctagt aaagggagta gttttaggtc cagtaaaaga atttaagtta aataatgaag
     4381 atgttaaggt aaatgaagat ggaacttttg tacataaagt tgctttaaaa gaaggaatga
     4441 ataaagttaa tatttatgcg aaagatgaaa atggaaatgt gttatataat tatgctagta
     4501 atatattatg tgatactaaa gctcctataa taaacttatc atctccaaag gtagaatcag
     4561 atggtatagt tataactaat gaagataaaa taaatataaa aggtactgtt gaggataaca
     4621 catggggata taaattctat aaaaatgaca ctattcagtt agaagttgaa gagagagcta
     4681 agccaggaaa tgatagtaca agaagagagt tttcatatga agttcctgta aaagatggag
     4741 atgttatagt attaaaggcc gttgatgtat taggtcatga aactcttaga aagcttactt
     4801 ttaaggttga taaaaatgct ctagaagtga caattggagg ggtatcagat caaggaatat
     4861 acaatagtga tgtaactcca aaggtagttt ctaatgaaga tgcagaaatt agttacttat
     4921 taaatggaaa agattatgat ggaaaaactc ctatttcaga ggatggaaac tatgagttaa
     4981 ttgtaaagtc taaagataaa gctggaaata aaacagaagt aaaaactaac tttactatag
     5041 ataaaacacc agcaaatatt tctgttaata atattgaaga tggaagagta tataatgaag
     5101 aaattattcc tgaaatagct agtaatgaag aagctacttt taaatatact ttaaacggga
     5161 aagaatatga tggtaagtct agtataaaag aagatggtga ctatgtttta aatatacaag
     5221 caacagataa agctggaaat gtatcaaata aagaagttaa gttttctata gatagaacac
     5281 ctgctaatat atttgtaact ggagttgaag agggtaaagt ttataatgaa cctgttactc
     5341 caataattga gattgatgag aaggatgcaa ctttaaaata tactttaaat ggaaaagaat
     5401 atgacggaaa atcaataata gatgaagatg gtaagtatat cttaaaggtt gaagctttag
     5461 ataaagcagg aaatccatca gaaaaagtta ttaactttgc tatagacaga agtttcttaa
     5521 aaaattcaga aaaggatgat ccaaataata ataagaaata taatgaacct attgatgagg
     5581 aaatagtaca aaagcctgaa gctaaaactg attcaaaaga ggaattaaag gctaataagc
     5641 ttaaagaaga gaataaagtt agtgaagaaa ataaaagtaa tgaagagaac tcagttaaag
     5701 atgaaaaact tcttaagaaa gaaggaacat tgccaacaac aggacaagtt cttggaggat
     5761 ctatgctatc tttattagga gctataatgg cttcagttgg agctgttttc ttaaaaagaa
     5821 aaaataaaaa caaggaagaa tagtaaatag aaagtattta aaataaatta ataaatttag
     5881 gatattttaa ggggattatt taaaaatata aagtagataa agagttttct tatagggtaa
     5941 acaaagaaat ttgtttatac ttaggagggc tctttatttt cttactaata aagagaatta
     6001 tcttataaaa aatcgattat attaagaatt ttagacttta ttttagtgta ataa
//
LOCUS       DAEVGI010000146         5954 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEVGI010000146 DAEVGI010000000
VERSION     DAEVGI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.146"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..2520
                     /locus_tag="K8M77_002141"
     CDS             <1..2520
                     /locus_tag="K8M77_002141"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012471.1,HMM:NF019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7086606.1"
                     /translation="TKQLALVQYPAGSVVRQGYVTNATNLIRYFKQGEWLNGSTTEPV
                     LDENGGSLGSLSPYEAATPLYKKNGMTHVVYDTSKGPNTKSGYVKFEGLPELPDTGIQ
                     IPSTSHSQATVEVYGTSGKGRPLKVYKIGSGSKVLFAGFALHGWEDNWSNDGLALVNI
                     ANSLIEKIGDYKAQNGLHGWTVYIAPCMNPDGVVVKGTHNGPGRCAVTTRVDMNRCFP
                     YKFSPQYISRNYTGPNPLGAPEAVALKNLVERINVSSSEMVVLDFHGWMNFTQGNAEL
                     GSYFGNQFGFGHKNLYSSGFFSSWATTLRNTKAVLIEYPTNTYSYNDVINKNYIGKTF
                     NGIINILKNNPNGGDVDNGGSDSSEIPYNANGEVINVEPTSALNVRSGPGTTYRAIGT
                     LKLGNRVTILAKTKPAGETKDWYKISFNYGSGYIRSDFVKLDSSEINYNAVGEIINVS
                     SFLNVRSGPGTNFEMLGKLYKGDVVLIVSKNGDWYKIRYGTTFGYIHKDYVYINKEND
                     NFVEKKVQIGAVEINKVKTIDGIVFANINQMLKGFCNNNGYTKAPSGDIIVTVHNFHK
                     KMDVSKTLPISKCKLINGDYYYNLNEFISILGYDEFVQNISDYSDVIYKYVYIGMDDE
                     TQFTIHEALDALGFIPVVGDVIDGVNAIMYALEKDYLNAALSLGAIIPVIGSPFFKGG
                     KYGFKIMSGFSKVANKSKKFVKKLDITEGRYVYRAIHDADLQRIKDGLGLQAKNPNGT
                     WSLEEHIVRGSNSESWKNDRFISTSLDLEISRIFNSSKNLGIIKIDLEKVPKELIYKS
                     YKEFPLYDINGHRSLAYEFSVWQQEISILKEIPRDAFEFIE"
     gene            2536..2823
                     /locus_tag="K8M77_002142"
     CDS             2536..2823
                     /locus_tag="K8M77_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086607.1"
                     /translation="MRNIRYVLTPEAYGSNGEFIDKIGTLGDLVVDTGMLLRPQFDKT
                     IPNIKVLNSLFREGWYPRSAEWEPFEIDSDEYNELVEYLLSLPLNKPYKLE"
     gene            2948..3499
                     /locus_tag="K8M77_002143"
     CDS             2948..3499
                     /locus_tag="K8M77_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HBI7086608.1"
                     /translation="MKYTLELICNEFNKNKIIDFLFFWGHTENNKKEVTKACLSQWYY
                     SYFKIDGIAYCCAEQYMMAEKARLFKDFNVLDKILAASKQSEIKRLGRMVKNFNENIW
                     NERKIDIVVKGNIEKFSQNEELKKFLLNTKNKIIVEASPYDSIWGVGLSVNNENITNP
                     NKWSGKNLLGFALMEVRDFLNEI"
     gene            4590..4958
                     /locus_tag="K8M77_002144"
     CDS             4590..4958
                     /locus_tag="K8M77_002144"
                     /inference="COORDINATES: protein motif:HMM:NF013826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H1/viroplasmin domain-containing protein"
                     /protein_id="HBI7086609.1"
                     /translation="MAKKNSKKYYAIKVGKGVKNKIVTTWKECSELVLGFNSIYKSFK
                     TREEAEQYLNGIKDNSIDIVLEKNKKAREYNTKKKKSTRSLQGIRIDKELYDRFQEKC
                     NKFEFTEEKAITLLIEEWLS"
     gene            5297..5590
                     /locus_tag="K8M77_002145"
     CDS             5297..5590
                     /locus_tag="K8M77_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBI7086610.1"
                     /translation="MATKVVKDDIIRVRVTKEHKEKLKEIAKKKNITISEIISVATEN
                     EIKKYEEKEKNYKKICERSVATEEKIQEIKLNLEKRKSKNKKNIFKNILNLRR"
     gene            5596..5856
                     /locus_tag="K8M77_002146"
     CDS             5596..5856
                     /locus_tag="K8M77_002146"
                     /inference="COORDINATES: protein motif:HMM:NF027249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI7086611.1"
                     /translation="MKKRYRFPCVLTKENDGITVTFPNFKGCISCGYTFEEAIKNGKQ
                     ALGLHINGLIEDNEVLPDSKEIKNIRVYENQKIEFIEVEIEE"
BASE COUNT         2360 a          571 c         1100 g         1923 t
ORIGIN      
        1 actaagcaat tagctttagt acaatatcct gctggtagtg ttgttaggca aggatatgtt
       61 acaaatgcaa ctaacttgat tcgttacttt aaacaaggag aatggttaaa cggaagtact
      121 acagagcctg tattagatga gaatggtggt tcattaggat cattaagtcc atatgaagct
      181 gcaacaccat tatataagaa aaatggaatg acacatgtag tatatgatac ttctaaaggt
      241 ccaaatacaa aatcaggtta tgtaaaattt gagggattgc cagagttgcc tgatacaggt
      301 attcaaattc cttcaacttc acattcacaa gcaactgttg aagtatatgg aacaagtgga
      361 aaaggaagac cgttaaaagt ttataaaata ggaagtggta gtaaagtact atttgcagga
      421 ttcgcgttac atggatggga agataactgg tcaaatgatg gacttgcatt agtaaatata
      481 gcaaattcat taatagaaaa gataggagat tataaagcac aaaatggtct tcatggatgg
      541 acagtatata tagcaccttg tatgaaccct gacggagttg tggttaaagg aactcataat
      601 gggccaggta gatgtgcggt aactactaga gttgatatga atagatgttt cccatataaa
      661 ttttcaccac aatatatttc aagaaactat actggaccta atccattagg agcaccagaa
      721 gctgtagcac ttaaaaattt agttgaaaga ataaatgtta gttcaagtga aatggtagtt
      781 ttagatttcc atgggtggat gaatttcact caaggtaatg cagagttagg aagctatttt
      841 ggaaatcaat ttgggtttgg gcataaaaac ttatatagct caggtttctt ttcaagttgg
      901 gcaacaactt taaggaatac aaaagcagtt ttaattgagt atcctacaaa tacttatagc
      961 tataatgatg ttataaataa aaattatata ggaaaaactt tcaatggaat aataaatatt
     1021 cttaagaata atcctaatgg tggagatgta gataatggtg gatcagattc gagtgaaatt
     1081 ccttataatg caaatggaga agtaattaac gtagaaccaa catcagcatt aaatgtaaga
     1141 agtggtccag gaacaacata tagagcaata ggaacactaa agttaggaaa tagagtaact
     1201 attttagcta aaactaaacc ggctggagaa acgaaggatt ggtataaaat aagttttaat
     1261 tatggaagtg gatatataag aagtgatttc gttaagctag actcatctga gattaattat
     1321 aatgctgtag gtgaaataat aaatgtatca tcttttttga atgtaagaag tggtccaggt
     1381 acaaattttg agatgttagg taaattatat aagggggatg tagttttaat agtatcaaag
     1441 aatggagact ggtataagat tagatatggt actacatttg gatatattca taaagattat
     1501 gtatatataa ataaggaaaa tgataatttt gttgaaaaaa aggttcagat tggtgcagtt
     1561 gaaattaata aggttaaaac tatagatggc atagttttcg ctaatataaa tcagatgtta
     1621 aaaggatttt gtaataataa tggttatacc aaagcaccat caggagatat tatagtaaca
     1681 gttcacaatt ttcataaaaa gatggatgtt tctaaaacat tacctatttc taaatgtaag
     1741 ttaataaatg gtgattatta ttataattta aatgaattca taagtatttt aggctatgat
     1801 gaatttgttc aaaatatatc tgattatagt gatgttatat ataaatatgt gtatatagga
     1861 atggatgatg agacacagtt tacaattcat gaagctttag atgcattagg gtttatacca
     1921 gttgtaggtg atgttattga tggtgttaat gctattatgt atgctttgga aaaagattat
     1981 cttaatgcag cacttagttt aggagctata attccagtta taggtagtcc attctttaaa
     2041 ggtggtaaat atggatttaa aataatgagt ggattctcaa aagttgctaa taaatccaag
     2101 aaatttgtca aaaaacttga tattactgaa ggtaggtatg tgtatagagc tatacatgat
     2161 gctgatttac aaagaataaa agatggctta ggattacagg caaagaatcc taatggaact
     2221 tggagtttag aagaacacat agttagggga tcaaattcag aatcatggaa aaatgataga
     2281 tttatatcaa catcgcttga tttagagatt tcaaggatat ttaattcttc aaaaaattta
     2341 ggtataataa aaatagattt agaaaaagtt ccaaaggaat taatatataa atcatataag
     2401 gaattccctt tatatgatat taatggacac agatctttag cttatgaatt ttcagtttgg
     2461 caacaagaaa taagtattct taaagaaatt cctagggatg catttgaatt tatagaataa
     2521 gtaaaaggag taattatgag aaatatacga tatgttttaa ctccagaggc gtatggttcc
     2581 aatggtgaat ttatagataa aattggtaca ttaggagatt tagtagttga tactggaatg
     2641 ctattaagac ctcaatttga taaaactatt cctaatatca aagtgttaaa ttctttattt
     2701 agagaaggat ggtatccaag aagtgctgaa tgggagcctt ttgaaataga ttcggatgaa
     2761 tacaatgaat tagttgaata ccttctaagt ttacctttaa ataaaccata taagttagaa
     2821 taatttatag agttttaata aatagaatat ataagcaatt ttagttaatt agaggacaaa
     2881 gttagtttac attgttctct aattttgtat ataaatattt aataagatag aatggtggat
     2941 taagattatg aaatatacat tagaattaat ttgtaatgaa ttcaataaaa ataaaataat
     3001 tgatttttta tttttttggg ggcatacaga aaataataaa aaagaagtta caaaagcttg
     3061 tttaagtcag tggtattact cttattttaa gattgatggt attgcatatt gttgtgctga
     3121 gcagtatatg atggctgaaa aggctagatt atttaaagat tttaatgttt tagataaaat
     3181 attagcagcc tctaaacaaa gtgagattaa aagattaggt agaatggtta aaaatttcaa
     3241 tgaaaatatt tggaatgaaa gaaaaattga tattgttgtt aaaggaaata tagaaaaatt
     3301 ttctcagaat gaggaattga aaaaattttt attaaataca aaaaataaaa taatagttga
     3361 agcgagtcca tatgattcta tttggggagt tggactatca gttaataatg aaaatataac
     3421 taatcctaac aaatggagtg gaaaaaatct actaggattt gctttgatgg aggttagaga
     3481 ttttcttaat gaaatttaga atataaaatt tcaatagtat aataagttat attatttttt
     3541 taaatgtaat agtatgaact tatcaatgaa ttaaaaatag agcgtagttt tggaaaaaat
     3601 attatagttt taaatatttt tatataataa ctaaaattta tagtttacat aataagaaaa
     3661 aaagcgtaaa attaagacat aaaatttcca attaaaattg ataattttag taaagggtag
     3721 aaattttgat tcaaaaataa aaaaaaagca cttcaaattg aaggctttat ataacaactt
     3781 cggttcaatt aattgtttgg cgacaattga ttgtaactta aggtattaca agtaccaaga
     3841 agctaaggaa taaacgaaca cttcaaacca gacctaaagt gaaggtaacc caaccgaaga
     3901 tcttatatct aattgatata catttagtat attaaaatga taataaattt tcaagaggtt
     3961 ttagtaattt tgtttacata attacatgtt tacaatagaa attctaagct taggtattaa
     4021 ctacctaagc tttttttgtt gatatataga atttaatata gaaaaaatcc tagttatagg
     4081 tgagtggtta aaggaataac actataaaaa actggtcggc tcaccaccga tagcagctcc
     4141 gttagatgga aatagcgact tcttagacaa gctatttaag ataatagttt cttagtagtg
     4201 gagtgcctgt cctggtggag tttgctttag ctcctagggg tgtgtgtaga gttacgtggt
     4261 agtatgcttg ataaaaccag acctaagcaa gaaagataca catagtggtt ggaagccacg
     4321 ggattaaatg cgacacggat acctcaatgg ctccaagcca ttaatataaa tgtttgctga
     4381 tcccctttgt agtatgtaat attttggaaa ttaaatctat ttatgtacta caaggggggg
     4441 gagcgagccg tattcctaat tcaccaaacc attaacagat gcttgattga aagtcaacaa
     4501 aaaaatacaa taaagttagc gagtgaaggg gtaaaaaaag gatatatagt atttataatt
     4561 atatttattt aagagagggt tatatagtta tggctaagaa gaatagtaag aagtattatg
     4621 caattaaagt tggtaaggga gtaaagaata agattgttac tacttggaag gaatgtagtg
     4681 aattagtatt aggttttaat tcaatatata aaagttttaa aactagagaa gaagcagaac
     4741 aatacttaaa tggaataaag gataatagta ttgatattgt attagaaaaa aataagaaag
     4801 caagagaata taatactaag aaaaagaaat caactagaag tttacaaggt ataagaattg
     4861 ataaagagct ttatgatagg tttcaagaaa agtgtaataa atttgagttt acagaggaaa
     4921 aagctattac tcttttaata gaagaatggc ttagttaaat attttgtagt taatatatta
     4981 tggcttgaat tgggataata tagggatttt atagggcgtg tttgtggctt agagtgggat
     5041 aattgtaaat tgttttgata tcattgaaat actaggcttt gcctagacta gaaccttgag
     5101 tgatagcaat ttctccttat gctctttaat aagctaaaaa aattattttc cagcttgttt
     5161 tctttgtaaa atctatttat ctaaatagac tttatttttt tatttttaat acaaaattta
     5221 tattgaaaat aaaggtttaa ctttgtagtt acagaattta attaagtaga agtatagata
     5281 aagtgaggat ttttttgtgg ctacaaaagt tgtaaaggat gatattataa gggttagagt
     5341 gaccaaagag cataaagaaa aattaaaaga aatagctaag aagaagaata ttactataag
     5401 tgaaattatt agtgtggcta cagaaaatga aataaaaaag tatgaggaaa aagaaaaaaa
     5461 ctataaaaaa atatgtgaaa gatctgtagc tacagaagaa aaaattcaag aaattaaatt
     5521 aaatttagaa aaaagaaaat caaaaaataa aaaaaatata tttaaaaata ttttaaattt
     5581 aaggaggtaa tttgtatgaa aaaaagatat agatttcctt gtgttttaac aaaagaaaat
     5641 gatggaataa cagtaacttt tccgaatttt aaagggtgta tttcttgtgg ctacactttt
     5701 gaagaagcta taaaaaatgg aaaacaagct ttggggttac atataaatgg cttaatagaa
     5761 gataatgaag tgctgccaga ttcaaaagaa ataaaaaata taagagtata tgaaaatcaa
     5821 aagatagagt ttattgaagt tgaaatagaa gaatagcaag tatagatttt gtcatgacac
     5881 tttaaagttg ttatatcaaa atcatcattt ttaggaataa tcctaagacc tatttaacac
     5941 aattaaattt aatt
//
LOCUS       DAEVGI010000147         5869 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEVGI010000147 DAEVGI010000000
VERSION     DAEVGI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.147"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..548)
                     /locus_tag="K8M77_002147"
     CDS             complement(<1..548)
                     /locus_tag="K8M77_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7086612.1"
                     /translation="MRNALKLNKMNYIDNIYSRSKGWITRSIINKRGYSQWHYKYAEL
                     KDLDMSDENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVLMDLEKNYF
                     NQSIPIPNYVIDSGRGMYLIWIINAVPSKALPLWKAVQEYLYNQLKFFGADRQALDAT
                     RILRVPGSINSKSKTVVNIVDE"
     gene            845..1201
                     /locus_tag="K8M77_002148"
     CDS             845..1201
                     /locus_tag="K8M77_002148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086613.1"
                     /translation="MKKGVKIFLSSMLLLGTIYTPAKAAYYTYGNGGDYIGKLNLYME
                     GSTRYGLAGTYIADNRYIVNTIKASLTDNGVLKATKSESMSSGATTKTVICNYSGVVG
                     THSVYSPSHGNWTGTT"
     gene            1258..2451
                     /locus_tag="K8M77_002149"
     CDS             1258..2451
                     /locus_tag="K8M77_002149"
                     /inference="COORDINATES: protein motif:HMM:NF014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086614.1"
                     /translation="MTILNIFFQYLKKQKLKKLQMFLQIFISLACVLLLIGMILNINN
                     KLTEIEELTSLDNIKGNFLIDNEYNSDPEIISNNLKNLTSDINKFNEIKFNQFSVQAG
                     LGSGYDIYIDSNMNDSIKFPIIKGRNFDKNDFKLDYEDKETIIPIIISKNLEQKYPLN
                     SEFKTKNNLFVNENNYINGGSTVKVVGILDNSAKFWLDDTILVDKLNFFDVIIYPLDF
                     QKNPQIISQYFINLNCSKDEYNNIKKEIEAKYPGIKLKDSDLKALFFDKLEDKIIELI
                     FISIFTIVLLITSLFGFISIIHSIILMREKELGVYYCLGASKKNILLFILMEIVIISL
                     LAAFICYISVYKFKDYFMYNFEVLLNKNTFLISLVIISLYILISNLVVSITILKKEPV
                     ELIKN"
     gene            2463..3563
                     /locus_tag="K8M77_002150"
     CDS             2463..3563
                     /locus_tag="K8M77_002150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086615.1"
                     /translation="MGIILKRWKKNHIKVFIIFLGMLLGILAISIGLSFLEDAYNFST
                     ELNGGNSKVKETLLYKNQEINEKNFTQDLENIVKELNKHYEVSLGSVIYPIDRNASLS
                     DAPSIKPVLYNKNIKWRPNVIYGRNLTLEESLSDEKVAVIGYEIFKNKFKNKEFENNL
                     TIDIYGETYKVIGVIGRTKRYSPQNFLIEIPYKNYFQIYKEEPDIQNIPIYISGNKDF
                     NYYNNKLTLLNKPKYINNIKMPLKLIILIGLLLLLVTIINESNLFSLWILNRKKEFAI
                     KKSLGSTNWLIIKDVLFEMILLSILVAFVALILQFFIQIELNQILYKYELKITLLNFI
                     VSIIVSLIVALITSIVPTKLIISINPCNELKS"
     gene            3582..4277
                     /locus_tag="K8M77_002151"
     CDS             3582..4277
                     /locus_tag="K8M77_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086616.1"
                     /translation="MKKPIIKVRNLSKEYLIDKKPFKVLDSINLDVYENDMIAIMGKS
                     GSGKSTFLNILGCIDKLSKGEYLLNDLRINDCKDKKLSEIRSKEIGFVFQNFNLINDY
                     TVLDNVKLGILYKKYYTKENLNAKAIALNYLKSVGMENKINKYPFQLSGGEQQRVAIA
                     RALASNPSIILADEPTGALDIENTENILKLLKEINQKGKTVIIITHDINVAKICDKIV
                     YLKDGKLINDDLK"
     gene            4261..5217
                     /locus_tag="K8M77_002152"
     CDS             4261..5217
                     /locus_tag="K8M77_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086617.1"
                     /translation="MTILNKKNNKTILFFIFILILGIGINKYFSKEDSNSFDAPISGL
                     GLFVFTEDNPQMHNQILDSSILNNYKYKFMNKTGKDLECNLNIFVNGIQINIQDIEND
                     SLSNNFKFFLKNNESKVLPIKPILDNLKNGLNIVNLNIITDNNTNAIDVNEKVWLKST
                     YNYTLEINNNGNELKKDDFILKQAKNYISIENLPNENQFIINNTKEDLEKEIAPKNFI
                     KAKKGSNVSLPIILGGGNSTELLLYGTLNNNQIIINNSLNLYYNLKENKYTIDNIEIK
                     APEKEGKYEMVFYGVFNLEKTNFNDINSIETRASHRITLIVE"
     gene            complement(5535..>5869)
                     /locus_tag="K8M77_002153"
     CDS             complement(5535..>5869)
                     /locus_tag="K8M77_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086618.1"
                     /translation="LNIIDRIKAKGASIKSIKDTWLDTSSDNPYNSFLLTVMSGLSQL
                     ERDLISQRTKEGLRSAKARGRNGGRPSKRNDKADTVCLLYREGYKIADIVNQTGLSRA
                     TIYRVLKD"
BASE COUNT         2364 a          670 c          666 g         2169 t
ORIGIN      
        1 tactcatcta ctatatttac tactgttttt gatttagagt ttatacttcc tggaactctt
       61 aatatcctgg ttgcatctaa ggcttgtcta tctgctccaa aaaactttaa ttgattatat
      121 aaatattctt gaactgcttt ccacaatggt aatgctttac taggtactgc atttattatc
      181 catattaaat acattcctct tccactatct attacatagt ttggtatagg aatactttga
      241 ttaaaataat tcttttctaa gtccattaat acctgatctt tagttttccc agttttataa
      301 tagtccaagt ctataaacag tgtatttaac tcttttatgt tttctaaccg cctacaaggc
      361 ttatagaagg tatttagagt tatatagata ttttcatcac tcatatctaa atcttttaat
      421 tcagcgtatt tatagtgcca ttgactatat ccccttttat ttataatact cctggttatc
      481 caccctttac ttctactata aatattatct atatagttca ttttattcag ctttaatgcg
      541 tttctcactt attcacctcc ccctttgtaa aactaagaaa attatatcat actttcaata
      601 ctgttaacta ttactaaata tagtatcctt attaaatgta tttataacga taattaataa
      661 ataaaaatac taccattaat taacacatta taattaataa gttattactt atcgtagtaa
      721 atacatattt ccccaaaatc aatccactaa attattatta tacaatttta tacaatagct
      781 tccaaaatcc ctctttatat gttataattt tactgttaaa ttttttaaaa aaaggagaaa
      841 atttatgaaa aaaggggtta aaattttttt atcaagcatg ttgttattag gaacaatata
      901 tactcctgct aaagctgcat attatactta tggaaatggt ggagattata taggtaagct
      961 aaacctatat atggaaggat ctactagata tggtttagca ggaacatata tagctgataa
     1021 taggtatatt gttaacacta taaaagcttc cttaactgac aatggtgttc taaaagcaac
     1081 taaatctgaa agtatgtcaa gtggagcaac tactaaaaca gttatttgta attatagtgg
     1141 tgtagtagga actcattcag tttatagtcc aagccatgga aattggacag gaacaactta
     1201 aaaaaacaaa aaaaatatga tattttaaat atcatatttt ttttataagg agactttatg
     1261 acaatattaa atatattttt tcaatattta aaaaaacaaa aattaaaaaa gcttcaaatg
     1321 tttttacaaa tttttatatc tttagcttgt gtattattat taattggaat gatattaaat
     1381 atcaataata aattaactga aatagaagag cttacttccc ttgataatat taaaggaaat
     1441 tttttaattg acaatgaata taactcagat cctgaaataa ttagtaataa tttaaaaaat
     1501 ttaacttcag atattaataa atttaatgaa attaaattta atcaattttc tgtacaagca
     1561 ggattaggta gtggatatga tatctatata gatagtaata tgaatgattc tataaaattc
     1621 ccaataatta aaggaagaaa ttttgataaa aatgatttca aattagacta tgaagataaa
     1681 gaaacaataa tacctattat aataagcaaa aatttagaac aaaaataccc tttaaatagt
     1741 gaattcaaaa ctaaaaataa tttatttgta aatgaaaata actatataaa tggtggctct
     1801 actgttaaag tagtaggtat tttagataat tccgctaaat tttggttaga tgatacaatt
     1861 ctagttgata aattaaattt ttttgatgta attatatatc ctcttgattt ccaaaaaaat
     1921 ccacaaataa tctcacaata ttttatcaat ttaaattgtt ctaaagatga atataataat
     1981 ataaaaaaag aaattgaagc aaaatatcca ggaattaaat taaaagattc agatttaaaa
     2041 gccttatttt ttgataaact agaagataaa attatagaat taattttcat ttcaattttt
     2101 actatagttt tattaataac ttctctattt ggctttatat caataattca ctcaataatt
     2161 ttaatgcgtg agaaagagct tggagtttat tattgcttag gagcttcaaa gaaaaatatt
     2221 ttattattta tattaatgga aatagttata attagtctat tagcagcttt tatatgttat
     2281 attagtgttt ataaatttaa ggattatttt atgtataact ttgaagtatt gctaaataaa
     2341 aatacttttc taatatcttt agtaataatt tctctctata tacttataag caatttagtt
     2401 gtttcaatta ctattttaaa gaaagaacct gtagaattaa tcaagaattg aggtgtactt
     2461 ttttgggaat tatattaaaa agatggaaaa aaaatcatat taaggttttt ataatttttt
     2521 taggaatgct tttgggaata ttagccattt ctatagggct ttcattttta gaagatgcat
     2581 ataatttttc aacagaacta aatggtggta atagtaaagt taaagaaact ttactttata
     2641 aaaatcaaga aattaatgaa aaaaacttta cacaagactt agaaaatatt gttaaggaat
     2701 taaataaaca ctatgaagtt tctttaggtt ctgttattta tccaatagac agaaatgctt
     2761 ctttatctga tgctccatct ataaagcccg ttttatacaa caaaaacata aaatggagac
     2821 caaatgttat atatggaaga aatttaacat tagaagaaag tttaagcgat gaaaaagttg
     2881 ccgtaatagg ctatgaaatt tttaaaaata aatttaaaaa taaagagttt gaaaataact
     2941 taactattga tatttacgga gaaacctata aagttattgg tgttattggt agaacaaaaa
     3001 gatattctcc tcaaaatttt cttatagaaa ttccatataa gaattatttt caaatataca
     3061 aagaagagcc tgatatacaa aatattccta tatatatttc aggaaataaa gattttaatt
     3121 actataataa taaattaact ttacttaata aaccaaagta tataaataat ataaaaatgc
     3181 cattaaaact aataatttta ataggattat tattactatt agttacaatt attaatgaaa
     3241 gtaatttatt ttctctttgg atacttaata gaaaaaaaga gtttgctata aaaaaatctt
     3301 taggatcaac taattggcta ataataaaag atgttctttt tgaaatgatt cttttgagta
     3361 tattggtcgc ttttgtagct ttaattctac aattttttat tcaaatcgaa ttaaatcaaa
     3421 ttttatataa gtatgaactt aaaataactt tattaaattt tattgtttca attatagttt
     3481 cattaatagt agcattaata acatcaatag ttccaaccaa attaataatt tctattaatc
     3541 cttgtaacga attaaaatct taaaagaaag tgagattaat aatgaaaaag cctataataa
     3601 aagtacgaaa tttaagtaaa gaatatttaa tagataaaaa gccttttaaa gtcttagatt
     3661 ctataaattt agatgtatat gaaaatgaca tgattgcaat aatgggaaaa tccggttctg
     3721 gaaagtcaac atttcttaat attttgggtt gtattgataa actttcaaaa ggcgaatatt
     3781 tattaaatga tttaagaata aatgattgca aagataaaaa attatcagaa atacgtagta
     3841 aagaaattgg atttgtattt caaaatttca acttaattaa tgattacacg gttctagata
     3901 atgtaaaact aggtatatta tataaaaaat attatacaaa agaaaattta aatgccaaag
     3961 ctattgcatt aaattattta aaatcagttg gtatggaaaa taaaattaac aaatatcctt
     4021 ttcaattatc aggtggcgaa caacaaagag tagctattgc aagagcatta gcttctaatc
     4081 ctagtattat tttagctgat gaaccaactg gagctttgga tatagaaaat actgaaaata
     4141 tattaaaatt attaaaagaa atcaatcaaa aaggaaaaac agtaattata attactcatg
     4201 atattaatgt agctaaaata tgtgataaaa tagtatattt aaaggatggt aaactaatta
     4261 atgacgatct taaataaaaa aaataataag acaattttat tttttatatt tattttaatt
     4321 ttaggtattg gaataaataa atatttttca aaagaagatt cgaactcttt tgatgcacca
     4381 atttctggat taggtttatt cgtttttact gaagataatc ctcaaatgca taatcaaatt
     4441 ttagattcat caattttaaa taattataaa tataaattta tgaataaaac aggaaaagat
     4501 ttagaatgta acttaaatat ttttgttaat ggtattcaaa taaatataca agatattgaa
     4561 aatgatagct tatctaataa ctttaaattt tttcttaaga acaatgaatc taaagtgctt
     4621 cctataaaac caatattaga taacctaaaa aatggtctta atattgtaaa tttaaatata
     4681 attacagata ataatactaa tgctatagat gtaaatgaaa aagtatggct aaaatccact
     4741 tataactata ctttagaaat taataataat ggtaatgaat tgaaaaaaga tgattttatt
     4801 ttaaaacaag ctaagaatta tatttcaatt gaaaatttgc ctaatgaaaa tcaatttatt
     4861 ataaataata caaaagaaga tttagaaaaa gaaattgcac ctaaaaactt tataaaagcc
     4921 aagaaaggtt ctaatgtttc tttacctata attttaggag gaggaaactc tactgaactt
     4981 ctactatatg gtacattaaa taataatcaa attataataa ataattcttt aaatttatat
     5041 tataatttaa aagaaaataa atatacaata gataatatag aaattaaagc tcctgaaaaa
     5101 gaaggtaaat atgaaatggt attctatgga gtatttaatt tagaaaaaac aaattttaat
     5161 gatataaact ctatagagac aagagcctct cacagaataa cacttattgt tgaataaaag
     5221 aaatctatgt aaaaactgaa aattttgtat cctatttaat caaatcgcct taaaatagga
     5281 tacaagatta attttaaatt taaactatta attgaaaaat ttatcttaaa ataacaaaat
     5341 aagtataatt ttattttgtt aaaacaactc tttatctaga atatctccaa aaatattgtt
     5401 agtttagcca tctcctacta aattaagttc attttttagt tataaactct aactaatctt
     5461 aatcattatg aaacattaat ttagtattaa taaccttaag aagttaacaa ttataacgtt
     5521 ccactaattg actattagtc ttttaaaact ctatatatag ttgctctact taatccagtt
     5581 tgatttacta tatcagctat tttataccct tctctatata gtaaacatac tgtatctgcc
     5641 ttatcattac gtttagaagg tcttccacca tttcgccctc tggctttagc acttcttaac
     5701 ccttctttag tcctttgact tattaagtcc ctttctaatt gactcaatcc actcataaca
     5761 gttagtaaaa aactattata tggattatca cttgatgtat ctagccaagt atctttaata
     5821 ctttttatac tagctccttt agcttttatt ctatctataa tattaagta
//
LOCUS       DAEVGI010000148         5867 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEVGI010000148 DAEVGI010000000
VERSION     DAEVGI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.148"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..2538)
                     /locus_tag="K8M77_002154"
                     /pseudo
     CDS             complement(1..2538)
                     /locus_tag="K8M77_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480024.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
     gene            2908..3213
                     /locus_tag="K8M77_002155"
     CDS             2908..3213
                     /locus_tag="K8M77_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086619.1"
                     /translation="MKIVKNLCNEVNLEDKCCKLCYRLIESYFNGTIVYGIEVERKDF
                     KDSNLINIERDSVCKISPNKDKVEVLVDKLYKYEVSPIHLIDIIGEDVDNCVADFIL"
     gene            3262..3663
                     /locus_tag="K8M77_002156"
     CDS             3262..3663
                     /locus_tag="K8M77_002156"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBI7086620.1"
                     /translation="MIIGIGVDIIEIGRVRQAIRNNKNFLSKLFTEREIDYFISRNMN
                     SEVIAGNFAAKEAVSKALGTGMRGFSFKDIEILRNELGKPEVILHNGANLIGNKLVEN
                     NNSLRVHLSISHNNSSAIAYSVLEGEYYGNI"
     gene            3650..5149
                     /locus_tag="K8M77_002157"
     CDS             3650..5149
                     /locus_tag="K8M77_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HBI7086621.1"
                     /translation="MEIFSSNSIRNMDKDYIEKFNMPSIVLMENAIIKFLENIDLTKE
                     SFTIISGRGNNGGDALGIARHLLNKGKKVYVHILEKEKEGSVDYKTNLTILENLGCYI
                     NSISNNKDLEILKNNIINSEIIIDGIFGTGLNKEIAGIYFDTIKCINENSKFTVSIDV
                     PSGINSDDGSICGIAIKANKTISFQVYKLGFLNYKSFEYLGDLKIVDIGIPKSIVYKN
                     SNNMRFIDKEYIRNIFPYRNKYGHKGIYGRSLIFAGQKGFIGAAYMVGEAAIKTGAGL
                     VTVFTHKDVQSELSIKLNEGMTGTYNSKEEYEEFIGGADVIAFGPGMGNNDLTFEVLN
                     NICNLYSGALVIDADGINVLERKKYILKEFKGDIVLTPHPGEMARLLGIEIKDVERDR
                     IGIAKKFAKENKVTVLLKGYNTIITDGDKVFVNPTGSSAMASGGMGDTLTGIITSLIS
                     QGVNSFDAAILGAYIHGYIGDELSKENFTVSAMEIIKNIPNKIKELINF"
     gene            5210..5803
                     /locus_tag="K8M77_002158"
     CDS             5210..5803
                     /locus_tag="K8M77_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086622.1"
                     /translation="MKGKRNAILIILLIIILGVIGGLAIYQRTYNLTPEDILEDMFSI
                     ESYETEIIYDVKNSRGQFQEKGRIYYDEEVGTKIVLDDREQLFEKDKIIINYVKDEKT
                     YEVGRDYDQFYRFMFINELKDLCNEEHEFTYKWNDENEKSEIILEFKNLNGNENFSRE
                     VMIIDAKKKVPKEAIIYDRDDLESVYIKFNNFSKVKN"
BASE COUNT         2152 a          693 c          964 g         2058 t
ORIGIN      
        1 catctcctaa atctattgtt aatgtattag gagcagagcc tacggcacac cacttagttt
       61 taacatttcc atctaaagca tattgaggag cttcattctc gtttacaaag cttgatgcct
      121 tagctgattt attaagtgat aagtttacta aaccatcttt tgcagcattt gcaacagtta
      181 taagttcagt tttagtttcc acattttctc cagaagaatt tttagcagta agtgttactg
      241 gataaacacc ttctttttca taagtaacag ttggattttg ttctgtacta ctttcaactt
      301 tagcacctgg gaatttcatt ggatttcttc tgtaacttca gaactagtgt tagtaaaagt
      361 tactgattgt cctggtgcta ttagtgtatt tgaaacttta aatgatgctg ttggagcagg
      421 gtaagcaggc cattccatat ttatactttg actatttcct ctgttatagt ttttattaac
      481 agcaactacc tcaaaattag tatttgtttc tttatcattt cttcttaaat cactaacata
      541 ataacttgta ttaggagttg cacctagtaa aactttagtt ccatcattca taactctgta
      601 aatttcataa tgatgtacct cagaagaatt tccttcggga ttccatgaaa gtctagctcc
      661 tgcatatatt ccatctttaa agtcaacatc atttaattta gcattagata tatttacttt
      721 tgaacttgag ttaggaattt ctattgttat attacctaag tttgcagaga agttatcaac
      781 acatactggg cttgaaagtt tataagaaat agttttaatt gattttccta catatggtga
      841 aatatcataa gtaagtttag tccaatcagt actaagtttc ttattagcag aaatagtagt
      901 ttctgtacca tcatggaatg taagaactaa atccatatct acagtatttc cattagcctt
      961 agcagtagta gtgaattgca caccttcagg taaagttaaa tcagaactat aaagttttat
     1021 tgtagaagct tttccttcat ctaggtaacc agaaagttta attgagttac caccatagta
     1081 agcagtagta taatctatgt cagcttttac attattattt tcttcatttg aaataaccca
     1141 tctgtaagtt ggcattatat cgtttaaact tctgttattc caatcttgag tagatacttt
     1201 attaccatca acataaaaat catatccatt ccccatatta aagttagttg taaatggaag
     1261 gctagttaca gcactttttt caacagagta tgttgaaatt tctctccaat caactccttg
     1321 agcattagtt gcctttgatg gatcaccatg ttcattaacc cataatctac tttcattttc
     1381 ttggaattgt tcctgtgttt tagcatcaaa ataagtccaa cttggacaat ataaacctaa
     1441 tgaagtaaat ggagcttggt catctctttg ataatgagtc catctaagag gagttttata
     1501 tccattttct tgaacatcta ttccagcata taagtcataa gggtttattc ctatttcttt
     1561 agctttttca ttagatactt ttaataaatc tttatctttt aaactattat atgtccacca
     1621 gaaatttaag aacatactat cagcaacttt gtttttatct ccatctaata agaaatattc
     1681 atttttatca gttaaagcat tttgccaatc catattccca tcttttgtca tggaatcata
     1741 ccacatgatt tcaaattttc cgttagactt agctttaaat tgccttatta attcttgcat
     1801 aagcttagca tgttcaggag ttaaaggttc ttgttcagtt ccttctgttt cttgatttat
     1861 gaaccaacca tcaaagccat agttttctgc aacttcaatt aacttatcta ttatagggaa
     1921 gtttccatta gaatcttttt caaggaattt atttaaccat tccatttttc caccatgagc
     1981 tgtcatagga aagaatacag tacctaaaac aggaacccca tttctgtgtg ctgagtcagt
     2041 aacatctgga cttggaggaa ctattaatcc ttctccagct gatccacccc agtaaaccaa
     2101 tttatctatg tattgccaat aagagaatgt attagcacta aatttatttg agccttgtga
     2161 tggattacca ctagtgttag cattcattat tgaaattgct acaactttaa catctttatt
     2221 ttgagtatca ttaatagtgt caagtttatc tttttcgact ctttttgcta atggaactgt
     2281 gcttttatta aattttgcat ctttatcatt gttagcactc cattttaata aatcttctgg
     2341 ataccaataa gatgagattg gttgattctg gattgagttt gaagtttctt cttgagtttt
     2401 cacataattt acaacacttg tatcttgttt aggtgttgcg taagctatct ttcctaaagg
     2461 attaaaagaa aatgcaaatg ctgcaaaaac acaaagtaaa gcccttctac gtttacgtat
     2521 tttttgtttg ttagtcataa aaatccccct aatatttaac ttgtatataa cgattataga
     2581 gagaattatt acataattag atattttcat aaatataact aattacataa taagtggata
     2641 aattatagaa tttatatcgt tttcataaaa tttattattg aattatgaaa agataaaaaa
     2701 ataaagataa aaaagaagaa attttcacag aaaaatacaa aaaaaatgaa atttgggttt
     2761 ctattaagag actcatgtcg ttaaataacg ataaaaaata tgtttatata tgcatgattt
     2821 gacatgtttc ttttgaaaaa gatgataata taaaggtaat taaatttcca ggtacataat
     2881 atttagggga aatagggagg agattaaatg aaaatagtaa aaaatctatg taatgaagtg
     2941 aatttagaag ataaatgctg caaactctgt tatagattaa tagaaagtta ttttaatgga
     3001 acaatagtgt atggaataga agttgaaaga aaagatttta aggattcaaa tttaattaat
     3061 atagaaagag attcggtttg taaaatatct ccaaataaag ataaggtaga agtattagtg
     3121 gataaattat ataagtatga agtgtcacct atacatttaa ttgatattat aggtgaagac
     3181 gttgataact gtgtagctga ttttatatta tagtagtatc atagtagata aatatattaa
     3241 tgattaaggg agaggatctc tatgataata ggtattggtg tggacatcat tgaaatagga
     3301 agagttagac aagctattcg aaataataaa aattttttaa gtaagttatt tactgaaaga
     3361 gaaatagatt attttattag tagaaacatg aatagtgagg ttatagctgg aaattttgct
     3421 gctaaagaag ctgtaagtaa ggctttagga actggtatga ggggattctc ttttaaagat
     3481 attgaaattc ttagaaatga attaggaaaa cctgaagtta ttttgcataa tggtgcaaat
     3541 ttaataggaa ataaattagt tgaaaataat aattcactaa gggtacattt aagtatctct
     3601 cataataata gtagtgcaat tgcatattct gttttggagg gtgaatatta tggaaatatt
     3661 tagttctaat agtataagaa atatggacaa agattatata gaaaagttta atatgccaag
     3721 catagtttta atggaaaatg ccataattaa atttttagaa aatatagatt taactaaaga
     3781 aagttttact attatttctg gaagaggcaa taatggtggt gatgccttag gtattgcaag
     3841 acatctatta aataaaggta aaaaagttta tgtacatata ttagagaaag aaaaagaggg
     3901 gtctgtagat tataaaacta atcttactat attagaaaat ctaggttgct atataaatag
     3961 tatttctaat aataaggatt tagagatact aaaaaataac ataataaata gtgaaataat
     4021 tattgatggt atttttggaa ctggacttaa taaagaaatt gctggaatat attttgatac
     4081 tataaaatgt ataaatgaaa atagtaaatt tacagtttct atagatgttc catcaggtat
     4141 aaattcagat gatggtagta tttgtggaat agctattaaa gctaataaaa ctataagttt
     4201 tcaggtttat aaattaggat ttttaaatta caaaagcttt gaatatttag gcgatttaaa
     4261 aattgtagat ataggaatac ctaagagtat agtttataaa aattcaaata atatgagatt
     4321 tatagataaa gaatacataa gaaatatatt tccttataga aataaatatg gtcataaagg
     4381 tatatatgga agatcattga tatttgctgg acaaaaagga tttattggag cggcttacat
     4441 ggtaggggaa gcagccataa agactggagc tggtttagtt acagttttta ctcataaaga
     4501 tgtccaaagt gaattatcta ttaagttaaa cgaaggaatg acaggaacat ataattctaa
     4561 agaagaatac gaagaattta ttggtggggc agatgtaatt gcttttggcc caggtatggg
     4621 taataatgat ttaacctttg aagtgttaaa taatatatgc aatctttata gtggagctct
     4681 tgtcatagat gcagatggaa taaatgtatt ggagagaaaa aaatacattt taaaagaatt
     4741 taaaggggac atagtattaa cacctcatcc aggagagatg gcaagattat taggtataga
     4801 aattaaagat gttgaaagag atagaattgg aattgctaaa aaatttgcaa aagaaaataa
     4861 agttacagtt ctattaaaag gatataatac aataataact gatggagata aggtatttgt
     4921 gaatcctact ggaagtagtg ctatggcatc agggggcatg ggagatacat taacaggaat
     4981 tataacttca ttaatatctc aaggagtaaa tagttttgat gcggctattt taggagcata
     5041 tatacatgga tacataggtg atgaattatc aaaggagaat ttcacagtaa gtgctatgga
     5101 aattataaaa aatattccta ataaaattaa agaattgatt aacttttaga ataaaaagga
     5161 ttactaatta atagtatgta aggtataagg ttttattggg aggattttca tgaagggtaa
     5221 aagaaatgcc atactcataa tactattaat tataatctta ggggttatag gtggactagc
     5281 tatctatcaa agaacttata atttaacacc agaagatata ttagaagata tgttttctat
     5341 agaatcttat gaaacagaaa taatatatga tgttaaaaat tctagaggtc agttccaaga
     5401 aaaaggtaga atttattatg acgaagaagt tggcacaaaa attgttttag atgatagaga
     5461 acaattattt gaaaaggaca aaataataat aaactacgtt aaagatgaaa agacttatga
     5521 agttggaaga gactatgatc agttctatag atttatgttc ataaatgaat taaaggattt
     5581 atgtaatgaa gaacatgaat ttacttacaa gtggaatgat gaaaatgaaa agtctgaaat
     5641 aatactagag tttaaaaatt taaatggaaa tgaaaatttt tcaagagaag taatgattat
     5701 agatgcaaag aaaaaagttc ctaaagaagc tataatttat gatagggatg atttagaaag
     5761 tgtttatata aagttcaata actttagtaa agttaaaaat taaaattaat attgtgattg
     5821 cttttttagc aatcacaata ttaatttatg taaaatattt tctttta
//
LOCUS       DAEVGI010000149         5455 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEVGI010000149 DAEVGI010000000
VERSION     DAEVGI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.149"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(514..1752)
                     /locus_tag="K8M77_002159"
     CDS             complement(514..1752)
                     /locus_tag="K8M77_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBI7086623.1"
                     /translation="MKAEIMAIGTEILLGDIVNTNAQFLAKELANLGIGVYHQSVVGD
                     NSERILEAFDNAFKNCDTIITTGGLGPTKDDLSKELAAKYFNMEMCLREELLCDLEDY
                     FKKNNLEMTENNKKQCYFPKEAIILPNPNGTAPGAILEGENNKRIILLPGPPREMEPM
                     FTNHVVPYLSKFTDSVLVSKILRVFGIGESKMDDLVCDLLDNENPTVAPYAKNIDVIL
                     RITAKGKDKEEAEKLIAPMEKEIRKRLGDNIYGEGEVTLEEVVGKLLVDKKMTVSTAE
                     SCTGGMVASTLINYPGISEVFMEGAVTYSNEAKMKRLGVKKETLEDFGAVSEECAREM
                     AKGIAKNAGTRIGISTTGIAGPGGGTEEKPVGLVYAGLCIDGITKVKKFNFKADRQKV
                     RTRTMMNVLDWLRRELEKID"
     gene            2076..2969
                     /locus_tag="K8M77_002160"
     CDS             2076..2969
                     /locus_tag="K8M77_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI7086624.1"
                     /translation="MNSKFKGIIFMILASAAFATMNLLSKLAVGVNSYQKTFLTNVVA
                     TLIVCFIIASKKGSFLGKKESRKYLLVRGVAGTLSILTNYYALDKMFLSDATILTKLA
                     PFFTIIFSYLLISEKITKKQFTLLIVAFAGSLFVIKPQFSTAIIPSLIGVASAAFAGI
                     AYTMIRVIGNKEDFWTIILSFTSIATIVMLPSMFMNTDNLTLQNILLLVGAGIAFTLG
                     QVALTIAYKNAPASEISMYDYIGLIIAAIYGFIFFKEIPDFLSFTGYIIIVGSSLLNI
                     LGNKKTSNKEINEDSSLVSTD"
     gene            complement(3190..3342)
                     /locus_tag="K8M77_002161"
     CDS             complement(3190..3342)
                     /locus_tag="K8M77_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086625.1"
                     /translation="MIGEVLSFGYDQCAKLGYAIGFGAMEGLVIYLGLLAIIVLKLFN
                     SKRKAN"
     gene            complement(3794..4810)
                     /locus_tag="K8M77_002162"
     CDS             complement(3794..4810)
                     /locus_tag="K8M77_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBI7086626.1"
                     /translation="MKKYLDKLQNIPIGISGTCLAFITLSNSWKLKGINYFKPIAIFL
                     AVCMLSLMLLRLIRFPKVMYEELKNPVTGTFYPTMGMVVWLVSGFFYPYFPVVCSLLW
                     IAAVIYHYGIVIFYTVIRIKERKFENIMPTCFIVYTGMITGSVASKGMDGVIPQIPQI
                     AHFMLMFGFILYTILLPLVLYIVFKSEILDDHKLPTVGIICSPAPLGVVGILTIEPNP
                     NPYMLAWLVITGLILLVVVYGYILKLFKEGFKPSYAAFTFPLAIATLSAFKLSTYFTG
                     LGYAKMANLFKFLGDVEIFIATYVIFFMLLNFLNMFFKAISPKLGEYVEEEEELIGGT
                     LYSK"
     gene            complement(5045..5371)
                     /locus_tag="K8M77_002163"
     CDS             complement(5045..5371)
                     /locus_tag="K8M77_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086627.1"
                     /translation="MSKYIYDENVFLKIDNFDNIYFEEKDSVLNIRLVKIEDGLVKEF
                     FEDDLKFDLSKYNNTKEELNKSLVYELKNEFLDFMDNEKEKIFDVIINLKELKEELIN
                     KEVSLV"
BASE COUNT         1898 a          898 c          564 g         2095 t
ORIGIN      
        1 tttacttact ttttattata ttaattttaa aacacataaa ttctataaat attttaataa
       61 gctatatcta tcatctttaa gtataataga cttaattttt ttcttaaact tttttcttaa
      121 aaataaaaaa ataaatataa aaaatatttt gttttaactg tataaaaaaa ataatactgt
      181 taataatatt tactgaggca ataagcttca atcttataat agcctctccc ccattttatt
      241 tatactgaca gtttcgttaa agtacggact aacgaaactg tctccccctt aaaaccttta
      301 taatgtaata aattttcttc atgtttttct agtagagagt attcatatac tctctcaatt
      361 tttaagtaag acatatttaa aagctaagct ttttaaaata aattattaga cactctaagc
      421 acatattaat tacccattaa gtttacttaa tgggtaattt ttttctcttt atatttataa
      481 gataaaacta aggtttttaa aacttaattt tctttaatca attttttcaa gctctcttct
      541 taaccaatct aaaacattca tcattgttct agttctaacc ttttgtctat cagctttaaa
      601 attaaatttt ttaacctttg taattccatc tatgcataat ccagcataaa caagacctac
      661 aggcttttct tctgttccac cgccaggacc tgctatccct gttgtagata ttcctattct
      721 tgttcctgca tttttagcaa taccctttgc catctctcta gcacactctt cgctaactgc
      781 accaaagtcc tctaaagttt ctttttttac tccaagtctc ttcatttttg cttcattaga
      841 ataagttaca gcgccttcca taaacacttc tgatattcct ggatagttta ttaatgtgct
      901 tgcaaccatg ccaccagtac aagattcagc tgttgatact gtcatctttt tatcaactaa
      961 taattttcca acaacttctt ctaaagttac ttccccttca ccatagatgt tatctccaag
     1021 tctctttcta atctcttttt ccataggagc aataagcttt tctgcctctt ccttatcttt
     1081 tcccttagca gtaattctta aaataacatc tatatttttt gcataaggag caaccgttgg
     1141 attttcatta tctaaaagat cacatactaa atcatccatc ttgctttctc caatgccaaa
     1201 aactcttaaa attttagata ctaaaacact atcagtaaat ttacttaagt atggtactac
     1261 atggtttgta aacattggct ccatttccct tggtggtcct ggtagaagta ttattctctt
     1321 attattttcc ccttctaaaa tagctcctgg agctgttcca ttagggtttg gaagtattat
     1381 ggcctccttt ggaaaataac attgtttttt attattctct gtcatttcaa ggttattctt
     1441 cttaaaataa tcctccaaat cacaaagaag ctcttctctt aagcacattt ccatattgaa
     1501 atactttgct gcaagttcct tgcttaaatc atccttagta ggtcctaacc cacctgtagt
     1561 aataattgta tcgcaatttt taaaagcatt gtcaaaagcc tctaaaatcc tttcagaatt
     1621 atctccaact actgattgat gataaactcc aattcctaag ttagctaact ccttagctaa
     1681 aaattgagca ttggtattta ctatgtcgcc taaaagtatt tctgttccta ttgccattat
     1741 ttcagctttc aaaaaaatca ccttcttaaa cttaatttat aattttattt ttgcaaaatt
     1801 atttattatc atctcccaaa taattttact ctactttctt ctctatgtcc tagtttttat
     1861 ttatattctt tctattattc ataattatta tataaatatt tttacaatta aatttgacct
     1921 cttattttct tagctctata atagtctttg tttatttata atacttttaa tttttattag
     1981 aatattaagg gtgttttaat aaacaatgca ataaatacta actaaaataa actataattt
     2041 gaagatctat ataaaataaa ttaaggagga aaaaaatgaa ttcaaaattt aaaggaatta
     2101 tttttatgat acttgcctca gcagcctttg ctactatgaa tctacttagt aagctagctg
     2161 taggagttaa ttcatatcaa aaaacttttt tgacaaatgt tgtagcaacc ttaatagttt
     2221 gctttataat agcctctaaa aaaggctctt ttttgggaaa aaaagaaagt agaaaatatt
     2281 tattagttag aggggttgca ggtactttat caatactaac aaattattac gccttagata
     2341 aaatgttttt atctgatgca acaatactaa ctaaattagc accatttttt acaataattt
     2401 tttcttactt attaattagc gaaaaaatta ctaaaaaaca atttacactt ttaatagtag
     2461 cctttgcagg aagcttattt gtaataaaac ctcaatttag tactgctata attccatccc
     2521 ttattggcgt agcctctgct gcctttgcag gaatagcata cacaatgata agagtgattg
     2581 gaaataaaga agacttctgg acaataatct tgtcatttac aagcatagct actattgtta
     2641 tgttaccttc aatgtttatg aacacagata atttaacact acaaaatata cttttacttg
     2701 ttggtgcagg aatagctttt acattaggac aagttgcctt aaccatagct tacaaaaatg
     2761 ctccagcatc tgaaatatca atgtatgatt acattgggct tattatagcc gctatatatg
     2821 gttttatttt ctttaaagaa attccagatt tcttatcatt tacaggttat ataataatag
     2881 ttggttcttc acttttaaat attttgggaa ataaaaagac ttctaataaa gaaataaatg
     2941 aagatagttc tttggtttct acagattaaa aatattattt aaaacaaaga aaagagctgt
     3001 gataaatcca cagccttttt ctttttgtcg aaaataaaaa attattattt tataatgaaa
     3061 tcaattaatt ataaattttt atcttataaa aatcaatgtt acaaaaccta aaaatttagt
     3121 attctaatct attgaagtat ttaacacttc tcaaatcaaa ttcattcaca aacatactta
     3181 ctaatgcacc taattagctt ttctcttact attaaaaagc ttcaatacaa ttatggctaa
     3241 aagcccaagg tatataacta acccctccat tgctccaaag cctatagcat aacctaattt
     3301 agcacattga tcgtacccaa aacttaatac ctctcctatc attttatgcc tcctaataaa
     3361 ctcttcatct aagatatatg aatttatatt ttataaaatg atgttttaat tataaaaaaa
     3421 gataattgga atgactaaaa catcatttca cttatctttt atattttcca aaaaaaatag
     3481 aaaatacagc atgacacatt attttggtag catcttataa aaagattatg agcagaggct
     3541 aaatttgttt attttattag tttgtaaagt aggtgggagt ttgacttact attaaagaat
     3601 tacccataat ctttattata aaatacttta aagtgattga gatttttatt atgttgttta
     3661 actaaaagtt tatcttataa caataataga tataagagtt tgtatctcta gaagtttaag
     3721 ccccattcta gaaatttctg actaaaattc aattgtgaat caagtcttaa gtttatatgt
     3781 aaactctatt caattatttt gaataaagag ttccacctat taattcttct tcttcctcta
     3841 catattctcc aagttttgga cttatagctt tgaagaacat atttaagaag ttaagtagca
     3901 taaagaatat tacatatgtt gctataaata tttcaacatc acctaagaac ttaaataagt
     3961 tagccatttt agcatatcct aaaccagtaa aataagttga taacttaaat gctgataaag
     4021 tagcaattgc taatgggaat gtaaatgctg catatgatgg tttaaaacct tctttaaata
     4081 gttttaaaat atatccataa acaacaacaa gtaatattaa tcctgttatt actaaccaag
     4141 ctaacatata tgggttaggg tttggttcta tagttaatat accaactact ccaagtggag
     4201 ctggtgaaca aattatacct actgttggta atttgtgatc atctaatatt tcacttttaa
     4261 atactatata taaaactaat ggtagaagaa ttgtatataa tataaatcca aacataagca
     4321 taaaatgtgc tatttgaggg atttgaggaa ttaccccatc cataccttta cttgctacac
     4381 tacctgttat catacctgta taaactataa aacaagttgg cattatattt tcaaactttc
     4441 tttcctttat tctaattact gtatagaaaa taactattcc ataatgatat attacagctg
     4501 ctatccaaag caaactacat actactggaa agtaaggata aaagaatcct gatactaacc
     4561 aaacaaccat acccattgta ggataaaatg ttcctgttac tgggttttta agctcctcat
     4621 acataacctt tgggaatctt ataagtctta aaagcattaa agatagcatg caaactgcta
     4681 aaaatattgc tattggttta aaataattta ttccttttaa tttccatgaa ttactcaatg
     4741 ttataaatgc caagcaagtt ccactaattc ctattggtat gttttgaagc ttatctaaat
     4801 attttttcat ctatctaatc accacttcct actcacttga tgattttatt atatctccct
     4861 tcccttataa gtaaaaatac ttactttata taattattat aagtatttag ttatatataa
     4921 agtcatgtta aaacatgatt tatgtagcaa agtctcttat ttaaatcttt gaaatactta
     4981 atttaagtaa attattataa aaaaataaga ttaagaggtt tatcttaatc ttatttctac
     5041 aaaactatac caaacttact tctttattaa ttaattcttc ctttagttcc tttaaattta
     5101 taattacatc aaatattttt tctttttcat tatccataaa atctaggaat tcattcttta
     5161 attcataaac caaactttta tttaattctt cctttgtatt gttatattta gataaatcaa
     5221 attttaaatc atcctcaaaa aactctttta ctaacccatc ttctattttg accaatctta
     5281 tatttaaaac agaatctttt tcttcaaaat aaatattatc aaaattatct atctttaaga
     5341 aaacattctc atcataaata tatttactca tattaattct tcccttcttt tgcttaaagc
     5401 taacatatta ttactaacat ataaattagt aaagatgtaa tcaaattctc ttaac
//
LOCUS       DAEVGI010000150         5454 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEVGI010000150 DAEVGI010000000
VERSION     DAEVGI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.150"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            141..1433
                     /locus_tag="K8M77_002164"
     CDS             141..1433
                     /locus_tag="K8M77_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7086628.1"
                     /translation="MKNLNLKGNPNRGLIGATLGFFIGFAAVSLYGPTSAVFKEAFVN
                     LNPVLLALLIAAPNLSGSLLRIPFSAWVDTTGGRKPLIVLLLLSIIGMGGLYVVLAFF
                     SDNLNQYYYLLFALGLLSGCGIATFSVGVSQASYWFPKSKQGVALGIYGGVGNLAPGI
                     FALLIPNLALPLLGLPGSYLAWLIFLIVGTVIYIKIGQNAWYFQLVDKGINKDEAKEI
                     ASKEYGQELFPKGKASETLLISAKSWKTWALVFIYFTTFGGFLALTGWFPNYWITYFG
                     LNMKVAGLLTALYSILTSLTRIYGGKVADKNGGEITTIVSLGIALIGAICMTFASTMT
                     LAIIGIILLAIGMGVANAAVFKIVPNAVPEAMGGASGWIGGLGALGGFLIPPVMASFL
                     NRSGFAGYSQGFSVFIVLIIIAIGVIALFQALQKKSAK"
     gene            complement(1799..3328)
                     /gene="guaA"
                     /locus_tag="K8M77_002165"
     CDS             complement(1799..3328)
                     /gene="guaA"
                     /locus_tag="K8M77_002165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBI7086629.1"
                     /translation="MRDLVLVVDFGGQYNQLIARRVRECGVYCEIIPYTYSIEKIKEK
                     NPKGIIFTGGPNSVYGENTPTLDKEIFNLNVPILGICYGDQLMAHLLGGKVATAPVRE
                     YGKTNVTLDNSSKLFAGIEADETCWMSHTDYIAEAPEGFKIIAHTDVCPVAAMENEER
                     RLYGVQFHPEVEHTPFGQNMMRNFLYNICGLENSWSMASFAEEKIAEIKKIVGDKKLI
                     CALSGGVDSSVAAVMVHKAVGKQLTCIFVDHGLLRKDEGDQVEKIFKEGFDMNLIRVN
                     AQDRFLGKLKGVSDPETKRKIIGEEFIRVFEEEAGKLGDIKFLVQGTIYPDVVESGTD
                     TSAVIKSHHNVGGLPEDMEFSLIEPLRELFKDEVRAVGEELGIPHHLVWRQPFPGPGL
                     AIRVLGEVTEDKLEVVREADAIFREEIALAGLESEIWQYFAVLPNIQSVGVMGDERTY
                     CHTVGLRAVTSSDGMTSNWAHIPYEVIDKVSRRIVNEVKGVNRIVYDVTSKPPATIEW
                     E"
     gene            complement(3439..4893)
                     /gene="guaB"
                     /locus_tag="K8M77_002166"
     CDS             complement(3439..4893)
                     /gene="guaB"
                     /locus_tag="K8M77_002166"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBI7086630.1"
                     /translation="MARILKTAYTFDDVLLVPNKSEVLPNEVSLKTQLTKKIQLNIPL
                     MSASMDTVTESKMAIAMAREGGIGIIHKNMTIEDQAREVDRVKRQENGVITDPIFLSE
                     DHTVREALDLMAQYRISGVPVTREGKLVGIITNRDIVFETNYDKKVSEVMTKSPLVTA
                     KEGTTLTEALEILKQHKIEKLPLIDDENNLKGLITIKDIEKAKAFPNAAKDEKGRLLC
                     GASIGVTNDMMERVDAVVKAKVDVIVLDTAHGHSKGVIEGVKRIKAKYPELQVIAGNI
                     ATPEAVRDLAEAGADCVKVGIGPGSICTTRIVAGVGVPQLTAVMDCAEEGKKLGIPVI
                     ADGGLKYSGDIVKALAAGACAVMMGSIFAGCEEAPGAIEIYQGRSYKVYRGMGSLGAM
                     AKGSSDRYFQNGTKKFVPEGVEGRIAYKGHLADTIYQLIGGIKSGMGYLGAPTLDNLY
                     ENANFVVQTSAGFRESHPHDINITKEAPNYSVNQ"
     regulatory      complement(5084..5181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         1651 a          962 c          729 g         2112 t
ORIGIN      
        1 taatttgtta ctatttttta ttttcgtgat aaaagttaca tattattttt tcatttagtg
       61 atataattta agtgtattta ttaaatattt ataaacttta gagttaaaaa attaacaatc
      121 aaaaaaacgg agtgaatgag atgaaaaact taaatcttaa gggtaatcca aatcgtggat
      181 taataggagc tacattaggt ttcttcatag gttttgcagc agtttctctt tatggaccaa
      241 cttctgcagt atttaaagaa gctttcgtaa accttaatcc agtattatta gcattactta
      301 tcgcagcccc taacttatca ggttctttac ttagaatacc tttttcagca tgggttgaca
      361 caactggtgg aagaaagcca cttatagttt tactactttt atcaataata ggtatgggtg
      421 gtttatacgt agtattagct ttctttagcg ataacttaaa tcaatattat tatttattat
      481 ttgcacttgg actattatct ggatgtggta tagcaacatt ctctgttggt gtcagccaag
      541 catcatattg gttcccaaaa agcaaacaag gcgtagcttt aggaatatat ggtggtgttg
      601 gtaacttagc acctggaata tttgcattat taattccaaa cttagcactt cctctattag
      661 gattaccagg ttcttactta gcttggttaa tattcttaat agttggtaca gtaatttata
      721 taaaaatagg tcaaaacgct tggtacttcc aacttgttga caaaggaata aataaagatg
      781 aagctaagga aatagcatca aaagagtacg gtcaagagtt attccctaaa ggaaaagcaa
      841 gtgaaacact tttaatatca gctaagtcat ggaaaacttg ggctttagta tttatatatt
      901 tcacaacttt cggtggattc ttagcattaa caggttggtt ccctaactat tggattactt
      961 atttcggatt aaatatgaaa gttgcaggtc ttttaacagc tttatattct atattaactt
     1021 cattaactag aatatatggt ggaaaagttg ctgataaaaa tggcggagaa ataactacta
     1081 tagtttctct aggaatagca cttatcggtg caatttgtat gacttttgct tcaactatga
     1141 ctttggcaat aataggtata atcttacttg ctattggtat gggagttgca aacgcagcag
     1201 tatttaaaat agttcctaat gcagttccag aggcaatggg tggagcttca ggatggatcg
     1261 gtggattagg tgctttaggt ggattcttaa tacctcctgt aatggcatca ttcctaaaca
     1321 gatcaggatt tgctggatat tcacaaggat tttcagtatt tatagtttta ataattatag
     1381 caattggagt aatagcttta ttccaagctt tacaaaagaa atcagcaaaa taaacattta
     1441 tataaaacaa aagtagctat gaattttcat agctactttt ttattttaaa ttataagtaa
     1501 ttaagtaatt tataaatata aaattatcca ccaaaattaa aactctaaat tatccacaaa
     1561 atattattaa gcccatttat aatctaatca caagtaatca tacattttta ttgatttaac
     1621 aagataaata gacaatcata ttaaaatgac tgcctattta tccgtaataa aaatttatat
     1681 atcttttgat tagtttggat acttaaattt tatctgattt agacctaata atttataatc
     1741 agataaatta tattaaaatt cagataggat taatacctat ctgaatcaat tttttatatt
     1801 attcccactc gatagttgct ggtggttttg aagttacatc gtaaacaatt ctgttaactc
     1861 ccttaacttc atttacaatt cttcttgaaa ccttatctat aacttcatat ggtatatgag
     1921 cccagtttga agtcattcca tctgatgatg taactgctct taatcctaca gtgtggcagt
     1981 aagttctttc gtctcccata acaccaactg attgaatgtt aggtaatact gcgaagtatt
     2041 gccatatttc actttcaaga cctgctaaag ctatttcttc tctaaatata gcgtcagctt
     2101 ctctaacaac ttctaattta tcttcagtta cttctcctaa aactcttata gctaatcctg
     2161 gtcctgggaa tggctgtctc catactaagt ggtgaggaat tcctaattct tctcctacag
     2221 ctcttacttc atctttgaat aattctctta atggctctat taatgagaat tccatatctt
     2281 ctggaagtcc tccaacgttg tggtgactct taattactgc tgaagtatct gttccacttt
     2341 caacaacgtc tggataaatt gttccttgaa ctaagaattt aatatctcct aattttcctg
     2401 cttcttcttc aaatactctt atgaattctt ctccgattat ctttctctta gtttctggat
     2461 ctgaaactcc ttttaattta cctaagaatc tatcttgagc attaactctg ataaggttca
     2521 tatcaaaacc ttctttgaag attttttcta cttgatctcc ttcatctttt cttaatagac
     2581 cgtggtcaac aaagatacaa gttaattgct tacctacagc cttgtgaacc ataacagctg
     2641 caactgatga atcaacccca cctgataatg cacatattaa ttttttatct ccaactattt
     2701 tcttgatttc agctattttt tcttctgcaa aagaagccat tgaccaagaa ttttctaatc
     2761 cacatatatt gtaaaggaaa tttctcatca tgttttgacc aaatggagtg tgctcaactt
     2821 ctggatggaa ttgaactcca taaagtcttc tttcttcatt ttccatagcc gctactggac
     2881 aaacatctgt atgagctata attttgaatc cttctggtgc ttctgctatg taatcagtat
     2941 gactcatcca acaagtttca tctgcttcta ttcctgcaaa taattttgat gaattatcta
     3001 aagttacatt agtttttcca tattctctta ctggagcagt agcaactttt ccacctaaaa
     3061 ggtgtgccat taattgatct ccataacata ttcctaatat aggaacattt aagttgaata
     3121 tttctttatc taatgtagga gtgttttctc cgtaaactga gtttggtcca ccagtgaata
     3181 ttattccttt tggattcttt tcttttattt tttctatgct atatgtgtaa ggtattattt
     3241 cacaataaac tccacactct ctaactcttc tagctattaa ttgattatat tgtccaccaa
     3301 agtcgacaac taaaactaaa tctctcattt gctttcctcc acaattttat tttccgttta
     3361 tgtataaagt atactcaaca aaatgttaaa acttataaga tatatttcaa cattttgctg
     3421 aagcttttaa tctaattatt attggttaac actgtagttt ggtgcttctt tagttatgtt
     3481 tatatcatga gggtgacttt ctctaaatcc tgctgaagtt tgaacaacaa aattagcatt
     3541 ttcatataaa ttatctaaag ttggtgctcc taagtaaccc attcctgatt ttattccacc
     3601 tattaattga tatatagtat ctgctaaatg tcctttgtaa gcaattcttc cttctacacc
     3661 ttctggaacg aatttcttag taccattttg gaaatatcta tcacttgatc cttttgccat
     3721 cgctccaagt gaacccattc ctctgtaaac tttgtagctt ctaccttgat atatttctat
     3781 agctcctggt gcttcttcac atcctgcaaa aattgatccc atcataactg cacaagctcc
     3841 tgctgctaaa gccttaacta tatctcctga atattttaat ccaccgtcag ctattactgg
     3901 tattcctaat tttttacctt cttcagcaca atccataact gctgttaatt gaggaacccc
     3961 aacacctgca actattcttg tagtacatat tgatccaggt cctataccta ctttgacaca
     4021 atctgcacct gcttctgcta aatctcttac agcctctggt gtagctatgt taccagctat
     4081 tacttgaagt tcaggatact tagcttttat tctcttaact ccttcaataa ctccttttga
     4141 atgaccatga gctgtatcta aaacaataac gtcaaccttt gctttaacta ctgcatctac
     4201 tctttccatc atgtcattag ttacaccgat tgaagctcca cataaaagtc ttcctttttc
     4261 atctttagct gcatttggga aagctttagc tttttctata tcttttatag ttataagtcc
     4321 ctttagatta ttttcatcat caattaaagg aagtttttct attttatgtt gctttaatat
     4381 ttctaatgct tctgttaatg tagttccttc tttcgcagta actaatgggc tttttgtcat
     4441 aacttctgaa acttttttat cgtagtttgt ttcaaatact atgtctctgt ttgttatgat
     4501 tccaactagt tttccttctc ttgttacagg aactcctgaa attctgtatt gagccattag
     4561 atctaaagct tctcttactg tgtgatcttc tgataagaat attggatctg ttattactcc
     4621 attttcttgt ctttttactc tatcaacttc tcttgcttga tcttctattg tcatgttttt
     4681 atggattatt cctattccac cttctctagc catagctatt gccattttag attcagtaac
     4741 tgtatccata cttgcactca ttaatggaat gtttaattga atttttttag ttaattgagt
     4801 ttttaaagaa acctcattag gtaggacctc tgatttgttt ggtactagta atacatcatc
     4861 aaatgtatat gctgttttta atattcttgc catactatcg cctccacaca ctatctttaa
     4921 gttatttacg tacaattaaa tgtttttccg tatttctacc ttttaaactt ctacttattt
     4981 tttgaaagca aacttgcaaa tatacacaaa agatgaaatt ttattttcaa aaaaaaaagc
     5041 ataataactc ctaagtgaag ttaatatgct tatagtacac ctatagtata tcaaattcac
     5101 tcatagacgg aaatttaagg tttccggtag aaacgccttg ccttattcaa agcttatacg
     5161 agtttgttta tattccgttt tgtttaattt ttatttatta taatctatga aagaaagctt
     5221 gtcaataaaa tgcataaaat aaatttcaaa agtttataaa tgattttttc taatcatttt
     5281 atatataatt tgcaagaaat aattcattta tttttatata atatattata tatttattgg
     5341 ttgtgttttt catattaata catatatttt ttattttatt atatatatct tacatgttat
     5401 ttataataat aaatattcca aacaaaatat tccctttatt tccctaactt taaa
//
LOCUS       DAEVGI010000151         5347 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEVGI010000151 DAEVGI010000000
VERSION     DAEVGI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.151"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            246..1817
                     /locus_tag="K8M77_002167"
     CDS             246..1817
                     /locus_tag="K8M77_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBI7086631.1"
                     /translation="MSNESTKKVLSHRKDLRFKLVLEGAAIGLLCSLVLVLNRIIVNY
                     LFPLFKKLYAWAALSITNMIIVLVILAILGALIGFMVRREPMISGSGIPQVEGELIGK
                     LKMNWLKILIYKFLGGVISLGAGLSLGREGPSVQIGAAVGEGFSNGLKRINIEKKYLI
                     TSGASAGLAAAFNAPLSGVIFALEEVHKSFSPLVLVSAMAASLVSDFVSKQFLGMNPS
                     LGMENVPVLPLKYYWVLIILGVILGIGGAIFSKGILKVQSLYGKFKNVPVEVKVMIPF
                     LLTGILGVFAPIILGGGHELIMDLATKSFPIKLLLAVLLVKFLLTIISFGAGTPGGIF
                     FPLLLLGAVVGNIVGIVSCNAFGVPNQYIIDFIILAMAGNFASIVKAPITGIILITEM
                     TGSFEHMLALSVVVIISYITSDLLKSEPIYESLLERWIEKVGSEVEEPSNNKTLLEVG
                     VELGSEAEGKEIKDVNWPETALLVAIKRGNNEIIPKGSIEIQSGDYLVVMVDESRASS
                     VLEEIQFLTSAKEEF"
     gene            complement(1951..2529)
                     /locus_tag="K8M77_002168"
     CDS             complement(1951..2529)
                     /locus_tag="K8M77_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBI7086632.1"
                     /translation="MKKVNFKGSVMLNPTPVVLVTSKNKEDKVNAFTVGWVSTVCTKP
                     PMIAMGIRPERLSHEYIKESKECVINLPCRDMVKFVDFCGVKSGRKVDKIKHFGYALD
                     KGIAVSTPSIEKAPVALECKLKSITPLGTHDMFLFEVLNVKVDESLIDEKGKIHFNKA
                     NLLAYSHGEYFGLNSKPLGSFGYSIKKKKKSR"
     gene            2687..3412
                     /locus_tag="K8M77_002169"
     CDS             2687..3412
                     /locus_tag="K8M77_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7086633.1"
                     /translation="MKKRFIFLLGIFLLVNSLSITLLEEKVLGKEKEEVTICIDAGHQ
                     EKGDRKLEPIAPWSNEKKPRVSSGTAGVGTKNKEYEINLEVAMILKELLNREGYKVVM
                     TREKNQVTLSNRERAEIGNASKADISIKLHCDGSNNSGKRGASILIPSSETKELKGIY
                     EESKKYGEILSETLKDGGVKVNGVFERKDMTGFNWSQRPVIILEMGFMSNWEDDALLG
                     DKLYQQKIADLIVKSLEKYRVEL"
     gene            complement(3649..4800)
                     /locus_tag="K8M77_002170"
     CDS             complement(3649..4800)
                     /locus_tag="K8M77_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBI7086634.1"
                     /translation="MKRKPKILLVTSLSLIVLLTLSIYVSLNKKKTSAFSEVINLLNE
                     PHQKEYDELKGKFEKVLQDLFKNRNIAILNNDLEELKKFYDLQKKPSLWAYESESKKV
                     KYLNNWSQKQGVVFNEIKSKIEIRKAREREKDLYGIICVVSSEFTYYYLNEPLKTNTF
                     RLGTYHYLNLKDEGDRCIITKEWYTDPFADSLDLNNIKSDEIKSYILNSSSPDYSPDE
                     RTQKAIDYAHTYCGAAADNELGFNYNKKYTDFNPQGGDCANFASQILFEGGGFKKNST
                     WNYSDGEGSKAWVNAQAFKNYMVNSGRTSYIAKGKYSEIYKAAYNLRPGDFVAYEKNG
                     RITHISTVTGLDSKGYPLVTCHNTDRLLVPFDLGWSNDNIRFHLVDVYY"
     gene            4969..5238
                     /locus_tag="K8M77_002171"
     CDS             4969..5238
                     /locus_tag="K8M77_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086635.1"
                     /translation="MKLVKGKIPFIRSIFGCFIFPLVIGYFYTYLNPEWFTTNQWVGV
                     AAGALLIARGIFEKVMANNQRNAKVSFLGGGAIILYVIARYFGIL"
BASE COUNT         1930 a          653 c          869 g         1895 t
ORIGIN      
        1 tataatcttc atattttaat actatgtata aaattttaag aaattacata ttttttaatt
       61 aataagattt tatattgaga aaataaaatt atgcaaaact attgctataa gaagagtgta
      121 aggtttacga tgagattttt tgaaaaaata atttgaaatt gaagtttaat taaatttgaa
      181 tctgtaaaaa cataagggta taataattat gttaataaaa tgttaaatgt aaggagggga
      241 aatctttgag taatgagagt acgaaaaaag tactgtcaca tagaaaggat ttaagattta
      301 aattagttct agaaggtgct gccataggac ttttatgttc tttagtttta gtactaaata
      361 gaattattgt taattactta tttccacttt ttaaaaagct ttatgcatgg gcagcattaa
      421 gtataactaa tatgattata gttttagtta tattagctat tttaggagca cttattggat
      481 ttatggttag aagagagcct atgatttcag gaagtggtat tcctcaagtt gaaggggaat
      541 taattggtaa acttaaaatg aattggctaa agattttaat ttataagttc ttaggaggag
      601 taatttcctt aggagctgga ttgtctttag gtagagaagg tccatcagta caaattggag
      661 ctgctgtagg agaaggattt agtaatggtt taaaaagaat aaatatagag aaaaaatatt
      721 taataacctc aggtgcttca gctggtttag cagcagcatt taatgcacct ttatcagggg
      781 taatatttgc cctagaagag gtacacaaga gtttctcacc tttagtttta gtttctgcaa
      841 tggcagcatc cttagtatct gattttgttt caaaacaatt tttaggaatg aatcctagtt
      901 taggaatgga aaatgttcct gtattacctt taaagtatta ctgggtatta ataatattag
      961 gagtaatttt aggtataggt ggtgcaatat tctcaaaggg aattttaaaa gtacaaagtt
     1021 tatatggaaa gtttaaaaat gttcctgtag aagttaaggt aatgattcct ttcttactaa
     1081 caggtatatt aggtgttttc gcaccaataa tactaggtgg tggacatgaa cttataatgg
     1141 atttagcaac taagagtttt ccgataaagc ttcttttagc tgtgctttta gttaaatttt
     1201 tattaactat tataagtttt ggtgcaggta caccaggtgg tatattcttc ccactattac
     1261 ttttaggggc agtagtagga aatatagttg gcatagtaag ttgtaatgca tttggagttc
     1321 caaatcaata tataatagat tttataatat tagctatggc aggtaacttt gcttcaatag
     1381 taaaggcacc tattacaggt ataatactta ttacagagat gactggttct tttgaacata
     1441 tgctagcttt atctgtagtt gtaataattt cctatataac atctgactta ctaaaaagtg
     1501 aaccaatata tgaatcttta ttagaaagat ggatagaaaa ggttggttca gaagtagaag
     1561 aaccttctaa taataaaact ctactagagg ttggagtaga acttggtagt gaagcagaag
     1621 gaaaagaaat aaaggatgtt aattggccag agacagcact tttagttgca ataaaaagag
     1681 gaaataatga aataatccct aagggatcta ttgagataca aagtggagat tatttagtgg
     1741 ttatggtgga tgagagcaga gcaagctctg ttttagaaga aattcaattc ttaacctctg
     1801 ctaaagaaga attttaatta tacatatgaa aagataatac taaatttaaa aaagagaatt
     1861 aataaactta tataggaatt ttagaaagac actaggaaaa tttatttgca caaattctcc
     1921 tagtgtcttt ttgaatatta gttaggaata ctatcttgat tttttctttt tcttaattga
     1981 ataaccaaaa gaacctagtg gtttagaatt aagaccaaaa tattctccat gactatatgc
     2041 taataaatta gccttattaa aatgaatctt tcccttttca tctataagac tttcatcaac
     2101 ctttacatta agaacctcaa ataaaaacat gtcgtgtgta cctaaaggtg ttatagattt
     2161 aagcttacat tctaaagcta caggtgcttt ttcaatagaa ggagtactta cagctattcc
     2221 tttatcaagg gcatatccaa agtgtttaat tttgtcaact tttcttccag attttacacc
     2281 gcaaaaatca acaaatttaa ccatatctct acatggtaag tttataacac attccttact
     2341 ttcttttata tattcatggg ataatctttc cggtcttatt cccatggcga tcattggagg
     2401 tttagtgcaa actgtgctaa cccaaccaac agtaaaagca tttactttat cttctttatt
     2461 tttagaagta acaagaacta ctggagtagg atttaacatt acacttccct taaaattaac
     2521 ttttttcata tgtatctcct ttaatttata ttaaattctt tattagacat aatttaagac
     2581 tttaaaatta taacatgaaa aatttatatg taaaaccagt ggataatatt ttttagtttt
     2641 tagagaaata ctatttatta tttaaatact agaaaggatt tttattatga aaaagagatt
     2701 tatattttta ttaggaattt ttcttttagt aaattcactt agcattactt tacttgaaga
     2761 gaaagtttta ggaaaagaaa aagaagaggt tactatatgt attgatgcag ggcatcaaga
     2821 aaaaggagat aggaagcttg aacctatagc accctggtct aatgaaaaaa agcctagagt
     2881 atcttcaggg acagcgggag ttggtacaaa aaataaggaa tatgaaataa acttagaagt
     2941 agctatgatc ttaaaagagc ttttaaatag agaaggctat aaagtagtta tgacaaggga
     3001 gaaaaaccag gttaccttaa gcaatagaga aagggcagaa ataggaaatg catcaaaggc
     3061 agatatttca ataaaacttc actgtgatgg atcaaataat tcagggaaaa gaggagcctc
     3121 aattttaatt ccttcttctg aaacaaagga gttaaaaggt atatatgaag aaagtaagaa
     3181 atatggagaa attttaagtg aaactttaaa agatggtgga gtaaaagtta acggagtttt
     3241 tgaaagaaaa gatatgacag gatttaattg gtcacaaaga cctgtaataa ttttagaaat
     3301 gggatttatg agcaattggg aagatgacgc tttattagga gataagttat atcaacagaa
     3361 aatagctgat ttaatagtta agtccttaga aaaatataga gtagaattat agttaaaaat
     3421 gatatgtatt aaaacttata aatatattct gagaattact gaattacgct aagaatttat
     3481 agctattact tcaaataatt tactaaagct cagaactccc gctggtcaaa cagcttcgct
     3541 tcttaagaca aattattcta cgtaataact taaattctaa agcttattca gatattctct
     3601 tcataatatt tataagtttt acttttaaga tatttatata tattcaaatc aataataaac
     3661 atctactaga tgaaagcgta tattatcatt gctccaacct aaatcaaaag gaacaagaag
     3721 tctatctgtg ttatggcaag ttactagggg ataaccttta ctatctaatc ctgtaactgt
     3781 tgaaatgtga gttattcgtc catttttttc ataagcaaca aaatcaccag gtcttaagtt
     3841 ataggcagct ttatatattt cagaatattt acccttagca atataggaag tacgcccact
     3901 attaaccatg taatttttaa atgcttgagc atttacccaa gccttagaac cttcaccatc
     3961 agaatagttc catgttgaat ttttcttaaa gccaccacct tcaaaaagaa tttgagaggc
     4021 gaagtttgca cagtctcctc cttgagggtt aaagtctgtg tattttttat tatagttaaa
     4081 accaagttca ttatctgcag ctgctccaca ataggtgtgt gcataatcta tagctttttg
     4141 tgttctttca tcaggtgaat aatctggact agaactattt aaaatatatg atttaatttc
     4201 atcagatttt atattattta aatctagaga atcagcaaaa ggatcggtgt accattcttt
     4261 agtgataata cacctatctc cctcatcttt taaatttaaa tagtgataag tacctaatct
     4321 aaaggtatta gttttaagtg gttcattaag ataataatat gtaaattcac ttgaaacaac
     4381 acatattatt ccgtataagt ccttttctct ttctctagcc tttcttattt caatttttga
     4441 ttttatttca ttaaatacaa ctccttgttt ttgagaccag ttgtttaaat acttaacttt
     4501 tttactttca ctttcatagg cccaaagact aggctttttt tgtaaatcat aaaatttctt
     4561 taattcctct aaatcattgt tcaatatggc tatatttcta tttttaaata agtcttgaag
     4621 tactttttca aattttcctt ttaattcatc atattctttt tgatgaggtt catttaataa
     4681 atttatgact tctgaaaatg ctgaagtttt ctttttgttt aaggatacat atatggataa
     4741 ggttaataat actattaggg ataaagaagt cactaataat attttgggtt ttcttttcat
     4801 taagccctcc tatttttaat acataattca ataatttata tgatgaataa ctaaaaaaaa
     4861 taataaataa aagtaaatat taacttaata ggagatatat gcttttaaat ttttaaaaat
     4921 gttgataaaa ttaatatgta ataagagagt aaaggagaat tacataagat gaaattagta
     4981 aaaggaaaaa ttccatttat aagaagtatt tttgggtgtt ttattttccc gcttgtaatc
     5041 ggatactttt atacatacct taatccggaa tggtttacaa caaatcaatg ggttggtgtg
     5101 gcagctggag ctttgcttat agctagagga atatttgaaa aagttatggc taataatcaa
     5161 agaaatgcaa aggttagctt tttaggtggt ggagcaataa ttttatatgt tattgctaga
     5221 tattttggaa tactataaaa attatataca taatttttag ttcaatattt taaaaaggaa
     5281 tattagtaaa cttaattaac cataaggaat aatcttaagc taaaagagaa atgatataga
     5341 tttagtt
//
LOCUS       DAEVGI010000152         5203 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEVGI010000152 DAEVGI010000000
VERSION     DAEVGI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.152"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            200..3127
                     /locus_tag="K8M77_002172"
     CDS             200..3127
                     /locus_tag="K8M77_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBI7086636.1"
                     /translation="MYYKIGIDAGSTTLKCIVLNEEDKILYSNYERHYSKVREKLIEE
                     LENIKGIIENKKFKIAITGSAGYGISKEYNLPFVQEVFATTLAIKRYYDDVDVAIELG
                     GEDAKIIFLTGGFEERMNSSCAGGTGAFIDQMAHLMGLTVDEMDNLSLKHENIYEIAS
                     RCGVFAKTDIQPLLNQGARKEDISASVFQSVVNQTIGGLAQGRSIEGKIMFLGGPLYF
                     CKGLRERFIKTLKLKDNDVVSPKNAQVFVAIGSAIYSKEDNKKYEYEELLNIIKENHN
                     EISTMNIIDPLFNSEEEYNEFKERHQKAEVKVSDISNYAGEAYIGIDAGSTTTKVVLM
                     DKDDNILYEYYGSNKGNPIEVVKKELKHIYNICGEKIKIMGSAVTGYGEELIKKAFSV
                     DFGIVETVAHYIAASHFNPKVDFILDIGGQDIKCFKIKDGIVANIMLNEACSSGCGSF
                     IESFANQMGYDVETFSKLGLFAKHGADLGSRCTVFMNSSVKQAQKEGATVEDLSAGLS
                     VSVVKNALYKVIRAKSLDELGKNIIVQGGTMYNDAILRAFEKELGINVIRPSISGLMG
                     AYGCALYAKRKNLKESKIISKEDLESFTHKSKIAKCGLCTNRCNLTINIFNDGEKYIS
                     GNRCEKPTGNKKSKSIPNMYQYKYKKLREYESLDEGKRGTIGLPMVLNMYENIPLWAT
                     FFKELGFKVVLSDESNKKMYLKGQHTIPSDTVCYPAKLAHGHIENLISKNIEHIFYPC
                     MSYNFDEGISDNCFNCPVVAYYPELLKTNVKELNSNNFMMEYLSLNNKKLLSKQLYEI
                     LNKTFGDIKQKEVKNALEKAYIEYEKYTKHIKEKGDEFIEYAKNNNKKVIVVAGRPYH
                     IDPEINHGIDKVLSSLGIVVLTEDSIEVSHEKPKVNILNQWTYQARLYNAAYYVAKNK
                     DMELIQLVSFGCGTDAITSDEVKDILERNNKIYTQLKIDETNNLGAAKIRIRSLLEAI
                     AQE"
     gene            3136..4401
                     /locus_tag="K8M77_002173"
     CDS             3136..4401
                     /locus_tag="K8M77_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBI7086637.1"
                     /translation="MEIENNKKFAVFTKEMRKTHTILAPTMLPIHFKLLSGIMKKYGY
                     KLEFFDGDTNSAIEEGLKSVHNDICYPAMIVIGQLMEALKSGKYDPDKTALIMSQTGG
                     GCRASNYIHLLRKALKENNLEQVPVISLNASGLEKHEGFKMYPGLLIKCIYALYYGDL
                     LMYMYNQCKSYEINKGETDRVLDKWIEILKEKFETLKYLKVRELYKNIILDFSKIELE
                     ETEKVKVGIVGEIYLKYSPLGNNNLEEFLREDNAEVVMSGVTDFFMYCLSNSEIDYKL
                     YGMKKVSSKFTKIGCSYLEHLQNIMIDSIKKYSKFRAPAPFKELKTSVEEYIGRGVKM
                     GEGWLMTAEMLELINSEVNNIVCAQPFGCLPNHIVGKGMIRGIMEKHPEANIVVVDYD
                     PSSTKVNQENRIKLMLANAKLSEYILNNN"
     gene            complement(4468..5046)
                     /locus_tag="K8M77_002174"
     CDS             complement(4468..5046)
                     /locus_tag="K8M77_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein"
                     /protein_id="HBI7086638.1"
                     /translation="MAAGKTHDKITVIMTPFIASFFLFINMSFLEDLGSSLAFTILGV
                     SVYMFGGYMFSGDLDIKSREFRRWGYLKFIWIPYQKLFEHRSVFTHGFILGPIIRILY
                     VSIIPITISSLLYSFSIINISTPEILKLVFNFILKNKILFFNMALALFLGSGLHTITD
                     MIYSYFKRKFSYKKRKSSKKYRKSKKPTRRAA"
BASE COUNT         2103 a          535 c          902 g         1663 t
ORIGIN      
        1 gagaatatgg agtaattttt aaaagtaaat ttaaattttg cttattatta aaacttttaa
       61 ttaggtgata taattaaata attatgatat agttaataaa gagttaagaa aaaagaatta
      121 tttctttaaa atttattcat atttttgcat gataattaat agaaatatat caattattaa
      181 taaagaggtg acattaactt tgtattacaa aataggtatt gatgcagggt ctacaacctt
      241 aaagtgtata gtcttaaatg aagaggacaa aatactttac tcaaattatg aaagacatta
      301 ctctaaggta agagaaaagc taatcgaaga actagaaaat ataaaaggga taattgagaa
      361 taaaaaattt aagatagcta taactggatc tgctggttat ggaatttcaa aagagtataa
      421 tttacctttt gttcaagaag tatttgctac aactttagct ataaaaagat attatgatga
      481 tgtggatgtg gcaatagagc ttggaggaga agatgccaag ataatttttt taactggtgg
      541 ttttgaggag agaatgaact caagttgtgc aggtggaaca ggagctttca tagatcaaat
      601 ggctcacctt atggggctta ctgtagatga aatggacaac ttaagtctta aacatgagaa
      661 tatatatgag attgcttcaa gatgtggggt ttttgctaaa actgatatac aacctctatt
      721 aaatcaagga gcaagaaaag aggatatttc agctagtgta ttccaatctg ttgtaaatca
      781 aacaataggt ggactagcac aaggaagaag tattgaggga aaaataatgt ttttaggagg
      841 acctttatat ttctgtaagg gattaagaga gagattcata aaaacattaa aattaaagga
      901 taatgatgtt gtttctccta aaaatgcaca ggtttttgtg gctattggaa gtgctatata
      961 ttctaaagaa gataataaaa aatatgaata tgaggaatta ttaaatataa taaaagaaaa
     1021 tcataatgaa atatcaacca tgaatataat agatccatta tttaattctg aagaagaata
     1081 taatgagttt aaagaaagac accaaaaagc tgaggttaaa gttagtgata taagtaatta
     1141 tgccggggag gcatatatag gcatagatgc tggaagtact acaacaaagg ttgttttaat
     1201 ggataaagat gataatattt tatatgaata ttatggatct aacaaaggaa atcctattga
     1261 agttgtaaaa aaagaattaa agcatattta taatatatgt ggagaaaaaa ttaagataat
     1321 gggtagtgca gttactggct atggagaaga gctaataaaa aaagcctttt cagttgattt
     1381 tggaatagta gaaactgtag ctcattatat tgcagcaagt cattttaatc ctaaggtaga
     1441 ctttatttta gatataggtg gacaagatat taaatgcttt aaaataaagg atggtattgt
     1501 ggctaatata atgctaaatg aggcatgttc atctggatgt ggttcattta tagaaagttt
     1561 tgctaatcaa atgggatatg atgtagaaac tttttcaaaa cttggtttat ttgcgaagca
     1621 tggagcagat ttaggatcaa gatgtacagt ttttatgaac tcatcagtaa aacaagcaca
     1681 aaaagaaggg gctacagtag aagatctttc agctgggctt tctgtaagtg ttgttaaaaa
     1741 tgctctttat aaagtaataa gagcaaaaag tcttgatgaa ttaggaaaaa atataattgt
     1801 tcaaggggga accatgtata atgatgccat ccttagagcc tttgaaaaag agcttggtat
     1861 aaatgttata agacctagta tatcaggact tatgggggct tatggatgtg ctctttatgc
     1921 aaaaagaaaa aatttaaagg aaagtaagat aatttctaaa gaggatttag agagttttac
     1981 gcataaatcc aaaatagcta aatgtggatt atgtactaat agatgtaatt taacaataaa
     2041 catctttaat gatggagaaa aatatatctc aggaaataga tgtgaaaaac caactggcaa
     2101 taaaaaatct aaaagtattc ctaatatgta tcaatataaa tataaaaaat taagagaata
     2161 tgaatcgtta gatgaaggaa aaagaggaac tataggatta ccaatggtat taaatatgta
     2221 tgaaaatatt cctctatggg ctacattctt taaagagtta ggttttaagg tggttttatc
     2281 tgatgaatca aataagaaga tgtatttaaa agggcaacat acaattccat cagatactgt
     2341 gtgttatcca gcaaaattag ctcatggtca tatagaaaac ttaataagta aaaatataga
     2401 gcatatattc tatccatgta tgagttataa ttttgacgaa ggtatatcag ataactgttt
     2461 taattgccct gttgtagctt attatccaga acttttaaag acaaatgtaa aagagttaaa
     2521 tagtaataac tttatgatgg aatatttaag cttaaacaat aaaaaattat taagcaaaca
     2581 actttatgag attttaaata aaacttttgg agatataaaa caaaaggaag ttaaaaatgc
     2641 tttagaaaag gcttatatag aatatgaaaa atatactaag catataaaag aaaaagggga
     2701 cgagtttata gagtatgcta aaaataacaa taagaaggtt atagtagtag caggaagacc
     2761 ttatcacata gatccagaaa taaatcatgg aatagacaag gttttaagta gcttaggaat
     2821 tgttgtttta acagaagatt ctattgaagt aagtcatgaa aagccaaagg ttaatatatt
     2881 aaatcaatgg acatatcaag ctagattata taatgctgct tattatgttg ctaaaaataa
     2941 agatatggag cttatacaat tagtttcctt tggatgtgga acagatgcca taacttctga
     3001 tgaagttaaa gacatactag agagaaataa caaaatatat actcagttaa aaatagatga
     3061 aactaataat ttaggcgcag ctaaaataag gataagaagc ttgctagagg ctatagcaca
     3121 ggaataggtg atagaatgga aatagaaaac aataaaaaat ttgcagtatt tactaaagag
     3181 atgagaaaga ctcatactat tttagctcct acaatgttgc caatacactt taaactttta
     3241 tctggaataa tgaaaaagta cggttataaa ttagaatttt ttgatggtga tacaaatagt
     3301 gccattgaag aaggattaaa aagtgttcat aacgatatat gctatccagc tatgattgta
     3361 attggacaac tcatggaagc tttaaaaagt ggaaaatatg acccagacaa aacggcttta
     3421 ataatgagcc aaactggggg aggatgtcgt gcatctaact atatacattt attaagaaag
     3481 gcactaaaag aaaataactt agagcaagtt ccagtaattt ctttaaatgc aagtggatta
     3541 gaaaagcatg aagggtttaa aatgtatcca ggcttattaa taaaatgcat atatgctctt
     3601 tattatggag atctgcttat gtatatgtat aatcagtgta aatcttatga gattaataag
     3661 ggtgaaacag atagagtttt agataagtgg attgagattt taaaagagaa gtttgaaacc
     3721 ttaaaatatt taaaggtaag agaactatat aaaaatataa ttttagattt ttctaagata
     3781 gaactagaag aaactgaaaa agtaaaggtt ggtatagtgg gagaaattta tttaaagtat
     3841 tctcctttag gaaataataa tttagaggaa tttttaagag aagataatgc tgaagttgtt
     3901 atgtctggtg ttacagattt ctttatgtat tgtctatcaa attcagaaat agattataaa
     3961 ttatatggaa tgaaaaaagt ttcaagtaaa tttactaaaa ttggatgttc atatctagag
     4021 catttacaaa atattatgat agattcaata aaaaaatata gtaagtttag agcacctgct
     4081 ccatttaagg aattaaaaac ttctgttgaa gagtacatag gaagaggagt taaaatgggt
     4141 gaaggatggc ttatgactgc tgaaatgtta gaacttataa atagtgaggt aaataatata
     4201 gtttgtgctc aaccctttgg atgtcttcct aatcatatag ttggaaaagg tatgattaga
     4261 ggaattatgg agaaacatcc agaagctaat atagttgttg tagattatga tccctcttca
     4321 acaaaggtaa atcaagaaaa tagaataaaa ttaatgttag ctaatgctaa attaagtgaa
     4381 tatatattaa ataacaacta agtataaggt ctcaaataaa aaaatttatt tgagacctta
     4441 atatttcaaa tttgaaaata atttttatca tgcagccctt cttgttggct ttttactttt
     4501 tctatatttt ttacttgatt ttctcttttt atatgagaat tttcttttaa agtaactata
     4561 tatcatatca gttatggtat gaagaccaga acctaaaaat aatgctaaag ccatattaaa
     4621 aaataaaatt ttattcttta atataaaatt aaaaactaac tttaaaattt ctggtgtaga
     4681 tatattaatt atggaaaaag aatataataa tgaagaaatt gttataggaa taatagatac
     4741 ataaagtatt cttattatag gtcctaatat aaatccgtgt gtaaaaacac ttctatgttc
     4801 aaatagtttt tggtatggaa tccaaataaa ctttaaatac ccccatcttc taaattctct
     4861 acttttaata tctaaatctc cagaaaacat atacccacca aacatatata cagatacgcc
     4921 taatattgta aaggcaaggc tacttcctaa gtcttctaag aagctcatat ttataaataa
     4981 aaagaaagaa gctataaaag gggtcataat aaccgtaatt ttgtcatgtg tttttcctgc
     5041 tgccaaaata tcaccccaca aaaaaatatt tgtaataata atataacata ttttgtgcta
     5101 atagcacata ttttattaaa ttaacaaatt aaaactttaa atttaagtta ttttatcata
     5161 atttatataa taacttaatt aataaaaaaa cttttgattt tct
//
LOCUS       DAEVGI010000153         5175 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEVGI010000153 DAEVGI010000000
VERSION     DAEVGI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.153"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(251..1072)
                     /locus_tag="K8M77_002175"
     CDS             complement(251..1072)
                     /locus_tag="K8M77_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI7086639.1"
                     /translation="MRITTLVENTKISAEYENKHGLSFHIETEKHNILFDLGPKNTFL
                     TNAKKLNINLEEVDIVVISHGHNDHGGGLEEFLKINNKAKVYIHKDAFNEYYSMAGIF
                     KKYIGLDKELKKNPRIILTEGDMKIDDELYLFSVVENRHKVSKFNKVLYKRVDGMYLE
                     DDFVHEQSLIITEDNKNVLMGGCAHNDIRNIIDKAEVIIGKDLDYVISGFHIFNPSTG
                     ISESDLFINTLGDNLNKRNTKFYTCHCTGMRAFKILEEKLQDKIEYISTGQVLNI"
     gene            complement(1321..1947)
                     /locus_tag="K8M77_002176"
     CDS             complement(1321..1947)
                     /locus_tag="K8M77_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086640.1"
                     /translation="MVFTINAYKIPLESVYRLKKNNNWEPQEHFLTIDFENDMIFNTH
                     EEAEKWLADNNILFINDEKVNTSEFQLNCYGVENFNIEIVVHRKTKPNIFTEKDVRKV
                     LNEGDDRYNNSLIIDFEGNLKLIQSNPEDIIYHSNYAVINEVYNSGNGFVGREFSDLY
                     IKYIYLNLLDNWVLHLESGRSIYVTCYEDNINEENTIYKINKLLADMN"
     gene            complement(2208..4006)
                     /locus_tag="K8M77_002177"
                     /pseudo
     CDS             complement(2208..4006)
                     /locus_tag="K8M77_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(4173..5168)
                     /locus_tag="K8M77_002178"
     CDS             complement(4173..5168)
                     /locus_tag="K8M77_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086641.1"
                     /translation="MINSNDKIILDSKLGNAISKEDRDEELKEKFPIELSGLRCIKTM
                     NSSFIDFYKTYEEEDLLLNKEDNLIQNFNLIESRKASSFSRSEKENNIPLNIANKFPI
                     ETEFLTLENLEHSNLLRIVDEEKESILIPYLLDKTPYLALLNKNYESYMLKHNFRGEG
                     FTIKDLFVLNLGKEVLVFIGFQVDTKLNKLHILKVKDGKFVKAFNENEYYYSKVFLEN
                     LNKDKEAELIIWINEIEKAYKIEIYSIKENGLKKTNKFDKEYYGKVVDYYKNLIESYG
                     NRNIYSYYLALAYEKRREYRKAIAVIDDALGEEFPYPSVEKLKELRKKIKNKLNS"
BASE COUNT         1705 a          860 c          387 g         2223 t
ORIGIN      
        1 ctactatata tgcaattaaa attattaaag ataggtttaa taaagtagca agtattctaa
       61 aaaaatttaa ttccatttta ctcctccaat ttactcttta tttatattaa tattttaatt
      121 aatttttatg atttttcaaa tttataaaat ttgtactatc acaggaaaat ttatacaata
      181 aataaacaag ggattatata aaatacttat attaaaattt ataagttatt ttgatacaat
      241 cccttgtatt ttatatattt aaaacttgtc cagtagaaat atattctatt ttatcttgta
      301 acttttcctc tagaatttta aatgctctca ttcctgtaca gtgacaagtg taaaacttag
      361 tatttctttt atttaagtta tctcctaatg tatttataaa taaatcactt tcacttattc
      421 ctgtgctagg attaaaaata tggaaaccac ttataacgta gtctaagtct tttcctatta
      481 taacctctgc cttatcaatt atatttctta tatcattatg agcacatcca cccattagta
      541 cattcttatt atcttctgtt attataagac tttgttcatg tacaaaatca tcttctaagt
      601 acataccatc tactctttta taaagtacct tattaaattt agatacttta tgtctatttt
      661 ctactactga aaataaatat aactcatcat ctattttcat atctccctct gtcaatatta
      721 ttcttggatt tttctttaat tctttatcta atcctatata ctttttgaaa attcctgcca
      781 tactataata ctcattaaaa gcatccttgt gtatataaac cttagcttta ttatttattt
      841 ttaaaaattc ttctaaccca ccaccatgat cattatgacc atgagatatt actacaatat
      901 caacttcttc taaatttata tttaacttct ttgcatttgt caaaaatgta tttttagggc
      961 ctaaatcaaa aagtatgtta tgtttttctg tttctatatg aaatgataag ccatgcttat
     1021 tttcatattc agctgatatt tttgtatttt ctactaatgt agttattctc aaattactca
     1081 ctcctcttta atctcaaact acctttatac taaaaacaac taaaaacaac tctgatcgca
     1141 tttttccttg caacaagtaa taactctcac tatttcatta ttctaatcag ttattatttg
     1201 taccatagac atttttatat cttctattta aatctttacc acacacggta caccttatta
     1261 tttcataatt ggaaaataca tttgactttt ccataatatt tgcatttcct ttatttataa
     1321 tcagttcata tcagctaaca atttatttat tttataaatt gtattttctt cattgatgtt
     1381 atcttcataa caagttacat aaatacttct tcctgattct aaatgtaaaa cccaattatc
     1441 taataagttt aaataaatat atttaatata caagtcgcta aattctcttc caacaaatcc
     1501 atttccgcta ttataaactt cattgataac agcatagtta gaatgatata ttatatcttc
     1561 tggattgctt tgtattaact ttaaattgcc ttcaaagtct attattaagg aattattata
     1621 tctatcatcg ccctcgttta aaactttcct tacgtctttt tcagtaaaaa tattaggctt
     1681 tgtttttcta tgtactacta tttcaatatt aaagttctca acgccatagc aatttaactg
     1741 aaattctgaa gtattaacct tttcatcatt tataaaaaga atattattat cagcaagcca
     1801 tttttcagct tcttcatgtg tgttaaatat catatcattt tcaaaatcaa tagttaaaaa
     1861 gtgctcttgt ggctcccaat tattattttt cttcagtcta taaactgact ctaaaggtat
     1921 tttataagca ttaattgtaa aaaccatatc ctcaccccta gtcattaaat ataaaaacaa
     1981 aaccaaataa ttacatttta attatatcat tctattaacc ttttgtacac atttacataa
     2041 tatataaaaa catctaattt cttaaatttg taaagtaact ccttattgtt aattaaaaaa
     2101 aaacaaacta acttcttatt tgctagtttg ctctttattt ttatttatta aattaattaa
     2161 aaattttata tttatcttta acaatattag aaaattataa gatatactta aattcttcct
     2221 aactctaaaa atttttctac aattatagga tcaaattgag taccagaatt ctttttaagc
     2281 tcctttttag cttcttcaga actaaggctt tttttataga ctcttttatt tgtcatagca
     2341 tcataagaat ctactacatt tataattcta gcaagtagag gaatttcatc accttttaaa
     2401 cctaatgggt atccagtacc atcatatctc tcgtgatgag ctaatataca tggtgctatt
     2461 ttttcaaact ctggattaaa catagcaatt ctatatcctt tttcagcatg agtttttatt
     2521 atttcatact cttctttttt taacttacct ggtttagata gtatttcctc aggaatccta
     2581 ctttgcctat atcatgaagt ttagctagca tttttaaatt ttttaattcc ttttctgata
     2641 agttcatctt cttacctagt tcaacagcat actttttaac tatatttata tgttctttga
     2701 tataatcatg tcttaaaaaa agctctttat ttaatgagtc tattatattc ttttttatgc
     2761 gtttttcatt caccaatttt tctctataaa ctttatcttc agcttctact aaaacttcat
     2821 ctattggttt tttagattct tttttactag atactccaag tgctatactt aaaggaatta
     2881 tagttttatg attaagctta caatttttcc ttattctgtt acatatagct tctgcttctt
     2941 cctctgttgt tttaggcaaa agaatacaaa actcatctcc tccccatcta aatataaggt
     3001 catcttttct acaagaattt tttattaccc ttgaaatttc ttttaaaatt ttatctcctt
     3061 ctaaatgtcc aatagcatca tttataacct taagtccatt taaatctccc attacaaaac
     3121 ttaaaggaaa aaactcttta ttatttaatt cttttaactt ttcatcgaaa taagctctat
     3181 tataaagccc tgtaagttta tctctataac tcattttttt taatttattt tctaaaataa
     3241 cattctcagt tacatctcta actaccccta caataccagt tactactcca cctttatcta
     3301 taacaggtac cttaatagac tctatatatc ttatctttct atttaattct atctttattt
     3361 tttcttttaa tttattcttg gtatttataa ctttatcatc ttgaagtata agcttatcta
     3421 gtattttttc ttccttaaaa ataccttttc tattggaagc ctttttataa atactcttta
     3481 tatcttttcc tattatttct gctttagtta tcttatagta atcttttact aaagtgttat
     3541 tacattttaa atacttacca tccttatctt tatgataaat tccatctgga atagattcta
     3601 caagagtgtt taaaagttcc ttttgattta ttaactcttc ctcatacttt tttctttcac
     3661 tttgattttg aagaattcct aaaaaatact ttatttctcc attttctttt ataggggcta
     3721 aataacactc taaaaatgat ccatcatgat atcctaatcc attttcatat cctgaaaaaa
     3781 gatttggctt ttcctcatct cttaccagat tataattttt aatatattca tctactacct
     3841 ctttagtata aatttcactt aaactcttac caattatata ttctttactc aaatttaaag
     3901 catttgagta atatttattc acaaaaataa atctaccatc tattcctatt aaccatactg
     3961 aataaggtat gttatctaat aatacctcta ataaatttga attcataaat ttcatccttc
     4021 tttacttata tactattaga atctaatttt atcataaaat aattatataa ttaaaccaaa
     4081 attattattt atattaagtt ttcaaaaata taaatagaaa aataactata tccatttaaa
     4141 ttctaaaatc tagatatagt tattttctat aatcaactat tcaatttatt ttttattttc
     4201 tttcttaatt cttttaattt ttcaacagat ggatatggaa attcttcccc taatgcatca
     4261 tcaattacag ctatagcctt tctatactct ctcctttttt cataagctaa ggctaaataa
     4321 taagaatata tattcctatt accataactc tctataagat ttttataata atctactact
     4381 tttccataat attctttatc aaatttatta gttttcttta atccattttc tttgatagag
     4441 taaatctcta ttttgtaagc tttctctatt tcgtttatcc aaataataag ttcagcttcc
     4501 ttatccttat ttaaattttc caaaaatacc ttactataat agtactcatt ttcattaaaa
     4561 gccttaacaa actttccatc ttttactttt aaaatatgaa gtttgtttag cttagtgtct
     4621 acttgaaaac ctatgaatac caatacttct tttcctaaat tcaaaacaaa taaatccttt
     4681 atagtaaaac cctcacccct aaaattatgt tttagcatat aagattcata atttttattt
     4741 aataaagcta aatatggagt tttatctaat aaataaggta ttaagatact ttctttttct
     4801 tcatctacta ttctaagaag attactatgc tctaaattct ctaaggttaa aaattcagtt
     4861 tcaattggaa acttatttgc tatattaagt ggaatattat tctctttttc acttctacta
     4921 aaggaacttg cttttctact ttcaataaga ttaaaatttt gtattaaatt atcttcttta
     4981 tttaataaaa gatcctcttc ttcataagtt ttataaaaat ctataaatga agaattcata
     5041 gtttttatac accttaatcc acttaactct ataggaaatt tttcttttaa ttcctcatct
     5101 ctatcctctt ttgatatagc atttcctaat ttagaatcta aaattatttt atcatttgaa
     5161 ttaatcatgt aaatt
//
LOCUS       DAEVGI010000154         5162 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEVGI010000154 DAEVGI010000000
VERSION     DAEVGI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.154"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(120..1067)
                     /locus_tag="K8M77_002179"
     CDS             complement(120..1067)
                     /locus_tag="K8M77_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBI7086642.1"
                     /translation="MNKVFCIGELLIDFIGKDIGKGLKNGVNFEKKAGGAPANVGAAV
                     CKLGGESYFLGQVGNDSFGEFLVDMLKNIGINTEMTKMDGYTTLAFVAIDEKGERDFE
                     FHRGSDGEYSFNNIDLSKIEKDDIIHFGSATGFLKGELKNTYFKLLEVGRKNGNFISF
                     DPNYRDMLIKEEGIEEFKEDCKKFISSCDFLKLSDEEIKLLTEEEDLERGVEKLHSLG
                     AKIIAVTLGGKGTLLSNGIHLEIVPSIKINQVDSTGAGDAFVGAVLKKVIELKDKKNL
                     DLNLWREIISYGNKVGALTCTNYGAIDAIPNEEDLREYI"
     gene            complement(1060..2523)
                     /locus_tag="K8M77_002180"
     CDS             complement(1060..2523)
                     /locus_tag="K8M77_002180"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBI7086643.1"
                     /translation="MEFLNDEDINKKSKEYYNKTKDIWRDNFHFEPPFGLINDPNGLS
                     YYKGEYYIFFQWNPYGCEHKNKHWGIVKTKNFKDFSIPKIALTPKEYFDKDGCYSGGA
                     IVKDGNLQVFYTGNVKGDKGERISYQCKGILEENGQLKKLGPVISTLPKGYTAHFRDP
                     YIWKNNGYYYMILGIQTEKLKGRCVIYKSSDLDDWKFYGELKTSYEDFGFMWECPSFL
                     KIDNKDIFIFSPQGIKEEEFRYQNIYQSGYLIGNLNYDTLEFKNNNEFKELDMGFDFY
                     APQVFKDEKGRNILIGWMGLPEKEEFHCSRENGWIFSLTMPRELTLKNGILYQNPIEE
                     MRKLRGDKIVSLKNTFIEGLNFSEVKENSYELKLDIERQEASKIEINFMKSDEEHTSL
                     IYDFKKEICIIDRNSMINGEKGTRKFKLNSNGNLRINMFVDKSSVEIYFQDGIEAASL
                     KLYPKKDSFNLSLKSEEGKIKINSLNVWEINEVNYNE"
     gene            complement(2563..3540)
                     /locus_tag="K8M77_002181"
     CDS             complement(2563..3540)
                     /locus_tag="K8M77_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI7086644.1"
                     /translation="MKVTIQDIANMVGVSKSTVSRYLNGGYVSNENTVKIKEAIEKTG
                     FETNFFAKRLKSKQSKLIGIIVPRIDSFTAGKTLNGINKKLEEQGYQGIILTSEFNIE
                     KELEQINKLYNQGVDGIIIMSFEISKEHVKLANKLPIPILFSGQKNDYINYLVLDDEK
                     IGNILGTYIKEQGHKDIVFLGVSESDREVGILRKKGFCNVLKKECNISFVETDFSFDK
                     AYEAGEEVLKYNPTAIVCATDNIALGLMRYLLENGKKIPEDISIAAFGGYEVSAITYP
                     SLTTVKIDYKLFGEEIALNILSLINGDKSINTCEIPLELMVRESVKSLK"
     gene            complement(3673..5025)
                     /locus_tag="K8M77_002182"
     CDS             complement(3673..5025)
                     /locus_tag="K8M77_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBI7086645.1"
                     /translation="MSKEQIVAQEILKNIGGKENIKSMEHCATRLRLIVKDKNLINEK
                     AIENIDGVRGQFFAAAQYQIILGTGFVNKVFAAMNGEGVETGNVKEDAYSDMTLPQKI
                     SRTLGDIFVPIIPVLVATGLFMGLRGLLTNLGVEFSPTFNTLSEVLTDTAFIFLPALV
                     AWSTMKKFGGTPVVGIVLGLMLVAPQLPNAWQVAGGANPIYISLLGISIPIVGYQGSV
                     LPALVLGIIAAKLEKFIRKFMPDVLDLIFTPFLTLLVSMILGLLVVGPIMHTSEVYIL
                     DLFKMFLNLPFGIGGAIIGGVHQVIVVTGVHHIFNALEVELISSTGLNPFNAVITGAI
                     VAQGAAALAVGFKTKDKKKRSLYISSAIPAFLGITEAAIFGVNLRFIKPFIFACIGGA
                     ASGMFASIMKLAGTGMGITAIPGTLLYINTGLIQYFITVAIGFAISFVLTYIFFKPQE
                     "
BASE COUNT         1671 a          987 c          497 g         2007 t
ORIGIN      
        1 atactcataa aattttattt taaaggataa agctctaatc taaaatacac taaaaaaaac
       61 atatgagaac taaaggattt taataggttc ctaaaaattc tcatatgctt tcaaataaac
      121 tatatatatt ctcttaaatc ttcttcattt ggaatagcat caatagcacc ataatttgtg
      181 catgtcaatg ctccaacctt atttccataa cttataattt ctctccataa atttaaatct
      241 aaattcttct tatcttttaa ttcaattact ttcttaagaa cagccccaac gaaagcatcc
      301 cctgctcctg ttgaatctac ttgattaatt tttattgatg gaactatctc taaatgaatt
      361 ccattactta aaagagttcc ttttcctcct aatgtaacag ctattatttt cgctcctaaa
      421 gaatgaagtt tttcaactcc tctttctaaa tcttcttctt cagttaatag ctttatctcc
      481 tcatcgctaa gctttaaaaa atcacaagag cttataaatt tcttacagtc ttccttaaac
      541 tcttcaattc cttcttcttt aataagcata tctctatagt ttggatcaaa agatataaag
      601 tttccatttt tacgtccaac ctctaaaagc ttaaaataag tgttcttaag ttcacctttt
      661 aagaatcccg tagctgaacc aaaatgaatt atatcatcct tttctatttt acttaaatca
      721 atattattaa atgagtattc tccatcacta cctctatgaa actcaaaatc tctttctccc
      781 ttttcatcaa tagctacaaa tgctaaggtt gtatatccat ccattttagt catttcagta
      841 tttataccta tattttttag catatcaact agaaattctc caaaagagtc attaccaact
      901 tgacctaaaa aataagattc tcctccaagc ttacaaactg ctgctccaac atttgctgga
      961 gctccaccag cctttttctc aaagtttact ccatttttaa gacctttccc tatatctttt
     1021 cctataaaat ctattaataa ttctcctata caaaatactt tattcattat aattcacctc
     1081 atttatctcc catacattta aggaatttat tttaatttta ccctcttctg attttaaact
     1141 taagttaaag ctatcttttt taggataaag ttttaatgat gctgcttcaa ttccatcttg
     1201 aaaatatatt tctactgatg atttatcaac aaacatatta attcttagat tcccatttga
     1261 atttaactta aacttccttg tgcccttttc cccgttaatc attgaatttc tatctattat
     1321 acaaatttct tttttaaaat cataaattaa ggatgtatgt tcctcatcac tcttcataaa
     1381 attaatttca atctttgatg cttcctgtct ttctatatct agtttaagtt cataactatt
     1441 ttcttttact tcactaaaat ttaaaccttc aataaatgta ttttttaagc ttacaatttt
     1501 atctccccta agctttctca tctcctctat agggttttga tataaaattc cattctttaa
     1561 agttaattct ctaggcatgg ttaaagaaaa tatccatcca ttctccctac tacaatgaaa
     1621 ctcttctttt tcaggaagtc ccatccatcc aattaatata tttcttcctt tttcgtcctt
     1681 aaatacttga ggcgcataaa aatcaaagcc catatctaat tccttaaact cattattatt
     1741 tttaaattct aaagtatcat aatttaaatt ccctattaag tatccagatt gatatatgtt
     1801 ttgatatcta aactcttctt ctttaattcc ctgaggagaa aatataaaaa tatccttatt
     1861 atcaattttt aaaaaggatg gacattccca cataaatcca aaatcctcat aagacgtttt
     1921 tagttcccca taaaacttcc agtcatctaa atcacttgat ttatatatta cgcatctacc
     1981 ctttagcttt tcagtctgta ttccaagaat catataataa taaccattgt ttttccaaat
     2041 atatggatct ctaaaatgag ctgtatatcc ttttggtaat gtagaaataa ctgggcctaa
     2101 ttttttcaat tgtccatttt cttcaagaat tcccttgcat tgataagata ttctctctcc
     2161 tttatcccct ttaacattcc cagtataaaa cacttgtaag ttaccatctt taacgatagc
     2221 tccccctgaa taacatccat ccttatcaaa atattctttt ggagttaagg caattttagg
     2281 tattgaaaaa tctttaaagt tctttgtttt tacaattccc caatgtttat tcttgtgctc
     2341 acagccatat gggttccact gaaaaaatat ataatattct cctttgtaat aacttaatcc
     2401 atttggatca tttataagcc caaagggagg ctcaaaatga aaattatctc tccatatatc
     2461 tttagtttta ttataatatt cttttgattt tttgtttata tcttcatcat ttaaaaactc
     2521 catttacttc acctttcttt aaattattcg gtgaagcaaa ccctacttta aacttttaac
     2581 actttctctt accataagtt ctaaaggaat ttcacaagta tttatacttt tatccccatt
     2641 tatcaaactt aaaatattaa gtgctatttc ttcaccaaat aatttataat caatttttac
     2701 agtggttagt gatggatatg ttattgcact tacctcatat cctccaaaag ctgcaatgga
     2761 aatatcctct ggtatcttct tcccattctc aagaagatat ctcataagcc caagagctat
     2821 attatctgtt gcacatacaa tagctgttgg attatatttt aaaacctctt ctcctgcttc
     2881 atatgcttta tcaaaagaaa agtctgtttc tacaaaactt atattgcact ctttttttaa
     2941 aacattacaa aatccttttt ttctaagaat tccaacttct ctatcacttt cactaacccc
     3001 taagaacaca atatctttat gtccttgttc ttttatatat gttcctaaaa tatttcctat
     3061 cttttcatca tctaaaacta aataatttat ataatcattt ttttgtcctg aaaacaaaat
     3121 aggtatagga agtttattag ctaattttac atgttctttt gaaatctcaa aagacattat
     3181 aataattcca tctacccctt gattatataa cttattaatt tgttctaatt ccttttctat
     3241 gttaaattca cttgttaata ttattccttg ataaccctgt tcttctagtt ttttatttat
     3301 accatttaat gttttacctg cagtaaatga atctattcta ggaactatta ttcctattaa
     3361 cttactttgt ttagacttta accttttagc aaaaaagtta gtttcaaaac cagtcttttc
     3421 tatagcctct tttatcttaa ctgtattttc attacttaca taaccaccat ttaagtatct
     3481 tgatacagta ctttttgata ctcctaccat attagctatg tcctgtattg taactttcat
     3541 cattttcctc ctaaataaag atatctatct tcttatatta taaattatat cattacctag
     3601 aagtttttaa ccatataaga ctattttgaa atatttaaaa gcatggctct gtaaaatcac
     3661 agagcctaaa aattattctt gtggtttaaa gaatatatat gttaatacaa atgaaatagc
     3721 aaatccaata gcaactgtta tgaagtactg aattaatcct gtattaatat aaagaagtgt
     3781 tcccggtata gctgttattc ccataccagt tccagctaat ttcattattg atgcaaacat
     3841 tcctgatgca gctcctccta tacatgcaaa tataaatggt ttaataaatc ttaagtttac
     3901 tccaaatata gctgcttctg taattcctaa aaaggctggt attgctgaag aaatatatag
     3961 tgaacgtttt ttcttatctt ttgtcttaaa tccaactgca agagctgctg ctccttgagc
     4021 tactatagcc ccagttatta ctgcattaaa tggatttaat cctgtacttg aaattaattc
     4081 aacttctaaa gcattaaata tatgatgaac tccagttaca actataactt gatgaactcc
     4141 tccaattatt gctccaccta ttccaaatgg taagtttaaa aacattttaa ataaatctaa
     4201 tatatatact tcacttgtat gcattatagg acctactact aaaagtccaa gaatcattga
     4261 tactaataaa gttaaaaatg gagtaaatat taaatctagt acatctggca taaattttct
     4321 aataaatttt tctaatttag ctgctataat acctaatact aaagctggaa gtactgatcc
     4381 ttggtaacct actataggga tacttattcc taataaagaa atatatattg ggtttgctcc
     4441 tcctgctacc tgccaagcat ttggaagctg aggggctaca agcataagcc ctaatactat
     4501 accaaccaca ggtgttccac caaatttttt cattgttgac caagctacta aggctggtaa
     4561 gaatataaat gctgtatctg ttaaaacctc tgataaagta ttaaaagttg gactaaattc
     4621 aactcctaaa ttagttaaaa gtcctcttaa tcccataaat aaacctgttg caactaatac
     4681 tggaattata ggaacaaaaa tatctcctaa agtacgagat attttttgtg gtaaagtcat
     4741 atcactatat gcatcttctt ttacattccc agtttctact ccttcaccat tcatagctgc
     4801 aaagacttta tttacaaacc cagttcctaa aattatttga tattgagcag cagcaaaaaa
     4861 ttgtcctctt actccatcaa tattctcaat tgccttttca tttattagat ttttatcttt
     4921 aactataagt cttaacctag ttgcacagtg ttccattgat tttatatttt cttttccacc
     4981 aatatttttt aatatttctt gagcaactat ttgttcttta ctcattttaa aaccccctaa
     5041 aatctattct cttttgatat cctttacttc ttgaatatat ggtatcagtt ccatatggtt
     5101 tctgtcaaac ttttaaattt atatcttaaa ataaattaaa ggcatatttt aatgaatttt
     5161 aa
//
LOCUS       DAEVGI010000155         5120 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEVGI010000155 DAEVGI010000000
VERSION     DAEVGI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.155"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..650)
                     /locus_tag="K8M77_002183"
     CDS             complement(<1..650)
                     /locus_tag="K8M77_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086646.1"
                     /translation="MENEFDIEFKNYLYKVLGKDNLQELESNDEDGECYLEIGSKLSI
                     DLSEGMVSLLTDKDSLLVILRFPSEAKKSFRGVSKGKVDFGFQLFKREDFDINYDFVG
                     FTLSIKDAVDYSDMRLDFNFTPEGFEFSEKMNYALNIIAIDEKYIVRAMRRIRFPREL
                     CKIIREYYLKITREDYNREVALEDLENLDAILFDGITSKQVEESFSLYKFTDKPEES"
     gene            complement(818..1240)
                     /locus_tag="K8M77_002184"
     CDS             complement(818..1240)
                     /locus_tag="K8M77_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI7086647.1"
                     /translation="MNYDSFEIAMLIKEIYSSTMGKVSEGLKESGLTHQQITVIKLIA
                     HKGKVTVKELCDEMSLAKGTVSGIVSRLENMGYVEKFKDEKDKRNTYIVFSKKGVDFA
                     KTFRCDINNSFDSVFENFTYEEVKEIREVLLKLREKIK"
     gene            complement(1394..1858)
                     /locus_tag="K8M77_002185"
     CDS             complement(1394..1858)
                     /locus_tag="K8M77_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086648.1"
                     /translation="MDGIKKFTPRVSKRILLFIAGLVWGFAGFRVFTLGFGDVMSHHG
                     NLIITIIFSAVIFFVFFKFIFSKMFKKHTTRIVNSKLDKHCVFSFFDFKSYLIMGFMI
                     FFGISVRNLGIFNPVYLGDFYMGLGAALFMAGVYFFVGFLTFDNLKVKYKIS"
     gene            complement(2307..2528)
                     /locus_tag="K8M77_002186"
     CDS             complement(2307..2528)
                     /locus_tag="K8M77_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086649.1"
                     /translation="MENKNIKLILVALGSFMLVLLQTEMFQRAIEIFSFIGLTIIGDI
                     ILLLSSILSFVGFVIFAFTSFKLIRNNIK"
     gene            complement(2830..4752)
                     /locus_tag="K8M77_002187"
     CDS             complement(2830..4752)
                     /locus_tag="K8M77_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBI7086650.1"
                     /translation="MRVKLVGLNEEMLEAVKGLEELIDFQLYNEKGSKNENLEIINVS
                     KLPEESENVIEVLRGKNNEIRYKKKHHFFRALSLYLQFLKKGEESFSRSEKTYIDSVG
                     VMIDASRNAIYRVSEVKRILGYMALMGHNRCMLYTEDTYEIEGYPYFGYMRGRYSKEE
                     LREIDDYGYSLGIEVVPCIQTLAHLKQTLRWPYGEGMKDTQDVLLVGEEKTYRFIEAM
                     ISSLRECFRSKNIHIGMDEAFDLGRGHYLTKHGPVSHQKLMVEHLNKVNEIAKKYDFK
                     PMIWDDMFLRCGAPDGGYYDLDIVITPEIANNIPEEVSLVYWDYYNSDEEKYKKLLDI
                     RDDFNNNIIFAGGCWRWSGFAPNYSKTFETTNAALNQCKAKGIKEVFATAWGDDGSET
                     PIYSIIVGLILFGEHGYYNKVEKEWIDERCKSLTGLSMEDFTSLEELDLVPTVKTPNM
                     EVCNPSKYIAYQDLLLGAFDKHLEGLDLEEHYINLSKKYEEIGERSERFKLMFTMYSK
                     LASYLSVKSEIGLEIRKAYLEKDKDALRLIAYNFIPEIQEKLKSFHKSFRDLWYKECK
                     GQGFEVIDIRLGGVMARCDSAIYRIKAYLKGNIDKIEELEEERLYFSEQFGGDDCKLI
                     CCNEYEKIATQNILSW"
BASE COUNT         1777 a          989 c          452 g         1902 t
ORIGIN      
        1 gattcttctg gtttatctgt aaacttatat aggctaaaac tttcttctac ctgctttgat
       61 gttattccat caaaaagtat cgcatcaagg ttttctaagt cttctagagc aacttctcta
      121 ttataatctt cccttgtaat ttttaaataa tactccctaa taatcttaca aagctccctt
      181 gggaatctaa tacgtctcat agctcttact atatactttt catctatagc aattatattt
      241 aaagcgtaat tcattttctc agaaaattca aaaccttcag gagtaaaatt aaagtctagc
      301 ctcatatcac tataatcaac agcatccttt attgataaag taaatccaac aaaatcataa
      361 ttaatatcaa agtcttctct cttaaaaagt tgaaatccaa aatcaacctt tcctttactt
      421 actcctctaa aactcttctt agcttcactt ggaaacctta aaataactag gagactatcc
      481 ttatccgtca aaaggctaac catgccttcc gaaagatcta tagaaagctt gctgccaatc
      541 tctaaataac actctccatc ttcatcatta gattctagtt cctgaaggtt gtcctttcct
      601 aaaaccttat acaaatagtt tttaaattca atatcaaatt cattttccat tctttaagtc
      661 gtctccttta tttttaaata atcttcaact attatatatc aacaacatta tagttacaaa
      721 ccatatttta accattttta attctaaaaa gactttaaaa atagcagaga catcatatac
      781 cataataatt aattaagttc ttctaaataa ctaagtatta ctttatcttt tccctaagct
      841 ttaaaagtac ctctcttatt tccttaacct cttcataggt aaagttttca aatacactat
      901 caaagctatt atttatatca cacctaaaag tttttgcaaa gtctactcct tttttagaga
      961 aaactatata tgtatttctc ttatcctttt catccttaaa cttctctaca taccccatat
     1021 tttcaagcct tgacactatt cctgacacag ttcccttagc taaggacatt tcatcacaaa
     1081 gttccttaac tgtaaccttc cccttatgag ctataagttt aatcactgtt atttgttggt
     1141 gagtaagtcc actttcttta agtccttcac ttactttccc catggtactt gaataaattt
     1201 cctttattag catagctatt tcaaatgaat cataattcat ataagtccct cttttctaaa
     1261 aaaataaatt tcaaattata tttccccaag gaatctaagt cctatgaaaa atatatgaaa
     1321 gattctacat ataaaattaa tggtacataa agccttctaa actttatgca ccatttaata
     1381 cctttatact aaattagcta attttatatt ttacttttaa attatcaaag gttaagaatc
     1441 ccacaaagaa atatacgcca gccataaata gtgctgctcc taatcccata taaaaatctc
     1501 ctaaataaac tggattaaat attcctaagt ttctaactga aattccaaag aaaatcataa
     1561 atcccattat aagatagctc ttaaaatcaa agaaggaaaa tacacaatgt ttatcaagtt
     1621 tactatttac tattcttgtt gtatgtttct taaacatttt actaaatata aatttaaaaa
     1681 acacaaaaaa tataactgct gaaaaaatga ttgttatgat taagtttccg tgatgtgaca
     1741 taacatctcc aaaccctaaa gtaaatactc taaatcctgc aaatccccac actaatcctg
     1801 ctataaaaag taaaattctc ttactaactc ttggagtaaa ttttttaatt ccgtccataa
     1861 ttttcatctc ctacaaaata attttatttt attagatgca acccatctta aaatgcttcg
     1921 catgaaaact ctttgttcct atgcaaacta ttttactcct aataataatt attgtcaaat
     1981 aaaagttttt atttatttca ttctttattt atgcagatca atcactgaaa ttaaggtact
     2041 aaaattttat tacttaaatt ataatctctt tcataagact cttattaacc ccaaaattat
     2101 cctttcaaag atttagtata aaagattttt ttaagggaaa cctaagtgtg tatccttata
     2161 gaggttaaga cttcattttt gtgattgatg ttcatcacct cctaaaagtc ttaatttctc
     2221 aaattttcta tatgcctttt taaattaaag atggctatac catacatttc ccccttctaa
     2281 gaaacttcaa gtatagccat ctatttttat ttaatattat ttcttataag tttaaaagat
     2341 gtaaaagcaa aaatcacaaa gcctacaaag gataaaatag aacttaataa aagaattatg
     2401 tcccctatta tagttaatcc aataaagcta aaaatttcta tggctctttg aaacatctct
     2461 gtttgaagta gcacaagcat aaaagatcct aatgctacta aaatcaactt aatattttta
     2521 ttttccattt aataatcctt ccctatattt caccttactt tttccctatt ttccacttga
     2581 aatatttatt tacaaaaatc aaaaatataa ctaataaaat gctcagtata aaaggctcta
     2641 aaccctcata caacctaata aaggtattca actaaaaaac tcctccttaa attttcacaa
     2701 actcaatagt actattttta aatcttatta tacctaataa gattaataat ttttttcata
     2761 tcataatatc cccaatttaa ttattttatc cattgcttat acttctgagt tttgtatcat
     2821 agaaactaat taccaactta aaatattttg tgttgctatt ttttcatatt cattgcaaca
     2881 tataagctta caatcatcgc ctccaaattg ttcagaaaaa taaagtcttt cctcttctag
     2941 ctcttctatt ttatcaatat ttcctttaag ataagctttt attctataaa tagctgaatc
     3001 acatcttgcc ataactccac caagtctaat atctataact tcaaagcctt gtcctttaca
     3061 ttccttatac catagatccc taaaactctt atgaaaactc tttagctttt cttgtatttc
     3121 tggaataaag ttatatgcta taagtcttaa ggcatcctta tccttttcta aataagcttt
     3181 tcttatttct agtcctatct cacttttaac tgaaaggtaa gaggcaagtt tagaatacat
     3241 agtaaacatt aatttaaacc tctcacttct ctctcctatt tcctcatatt ttttagaaag
     3301 attaatataa tgttcttcta aatctaaacc ctctaaatgc ttatcaaagg ctcctaaaag
     3361 taaatcttgg taggctatat attttgaagg attacaaacc tccatattag gagttttaac
     3421 tgttggaaca agatctaact cttccaaaga agtaaaatcc tccatgctaa gccctgttaa
     3481 agatttgcat ctttcatcta tccattcttt ctctaccttg ttataatagc catgctctcc
     3541 aaataatata agacctacta ttattgaata tattggggtt tctgatccat catctcccca
     3601 agccgttgca aagacttctt ttattccttt agccttacat tgatttaagg cagcatttgt
     3661 tgtttcaaag gtctttgaat agtttggagc aaatccactc catctccagc atcctcctgc
     3721 aaagataata ttgttattaa aatcatctct aatatctaaa agctttttat atttttcttc
     3781 atcagaatta taataatccc aataaactaa ggaaacttcc tctggaatat tatttgctat
     3841 ttcaggagtt ataactatat ctaaatcgta atatccacca tctggagctc cacatcttaa
     3901 aaacatatca tcccaaatca ttggcttaaa atcatatttt tttgctattt cattaacctt
     3961 atttaaatgc tctaccataa gcttttggtg agaaacaggt ccatgtttag ttaaataatg
     4021 gcctcttcct aaatcaaaag cctcatccat acctatatga atatttttac ttctaaaaca
     4081 ttctcttaat gaagaaatca ttgcctctat aaacctataa gtcttttcct ctcctactaa
     4141 aagtacatct tgagtgtctt tcatcccttc cccataaggc catcttaaag tttgttttaa
     4201 gtgagctaaa gtttgaatgc aaggtacaac ttctatacct aaagagtatc cataatcatc
     4261 aatttccctt aactcctctt tgctatatct tcctctcata taaccaaagt atggataacc
     4321 ctcaatctca taagtatctt cagtataaag catacaccta ttatgtccca ttaaggccat
     4381 ataccctaat atccttttaa cctcagaaac tctataaata gcatttcttg aggcatctat
     4441 cattacccct actgaatcaa tataagtttt ttcacttctt gaaaaactct cctctccctt
     4501 tttaagaaat tgaagatata aactaagtgc tctaaaaaag tggtgttttt tcttatatct
     4561 tatttcatta ttttttcctc taaggacttc tattacattt tctgattctt caggaagctt
     4621 agaaacattt ataatctcta aattttcatt cttactcccc ttctcattat aaagctgaaa
     4681 atcaattagt tcttctaatc ccttgacagc ttctaacatt tcctcattta atccaactaa
     4741 tttaactctc atctttattc ccctctcata caaaaatagg cgtttcctat ataattccac
     4801 attaaaatca aatctcctac taaaaagatt attttataat aaataaaaat aaattgttat
     4861 aaaaatgaac tataaaacat gaaaataaat ctaaacattg ttttagcaaa taaaaattat
     4921 tctaagaatg gactatctaa gatcctctaa tagtttttaa aataaataaa ataacttatg
     4981 cataaataac tacacatata aataaaactt ataaatagat aaagagctta tattcttaaa
     5041 ctctaaatct atttataagt ttttaatata tgtatcaata atacttaagc ttattaaatt
     5101 tataatagat tttctttata
//
LOCUS       DAEVGI010000156         5046 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEVGI010000156 DAEVGI010000000
VERSION     DAEVGI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.156"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            134..1108
                     /locus_tag="K8M77_002188"
     CDS             134..1108
                     /locus_tag="K8M77_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7086651.1"
                     /translation="MWFKKLYVGTIDLLSAIVEKIAKCNLACKIYKAVFQLGVFPKPK
                     NFNEIVSNLVIEKDIIYESSILEGNKLDVYYPKNLNKKAPILMWIHGGGYIANSKETV
                     KNYMMTLANKGFVVFNIDYALAPKYKYPSQIVQCNEALKYVFENAEKFNGDRENIFIG
                     GDSAGAQMASQLVAIISNEELSNKMNLKPSITNKFLRGVILFCGLYNMDSLRETKFPG
                     IKTYMEALTGESDFENYERIDELSTIKYITPNYPSTFITVGDIDPFVDQGKELASSLR
                     ALGVNVVSRFFENKGLWHEYQFKFSIPESRETFKSIIKFLNDNSKSSN"
     gene            complement(1319..2110)
                     /locus_tag="K8M77_002189"
     CDS             complement(1319..2110)
                     /locus_tag="K8M77_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086652.1"
                     /translation="MKRYARIYVQFLKQYLKTLLEYKEDFIYGILGFFLVQATSIIFI
                     SLIFNNVPTLSGWSFYEIIFIYGFSQIPRGIDHIFTDYLWIFSGRSIVRGEFDRYLLR
                     PLNPLFQVIAERFQPDGFGELVVGFILVVYSAIKLSVNITLLWIIGFIIAVIGGTLIY
                     TGVKLATASIAFWTKTSFRHVQMAYGLSDFSKYPISIYPGAIKFLLTFIIPFAFTGYY
                     PGTYLLGKESFFMGVILTLIVGILSIVVSYRIWLKGISSYESSGN"
     gene            complement(2115..2933)
                     /locus_tag="K8M77_002190"
     CDS             complement(2115..2933)
                     /locus_tag="K8M77_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086653.1"
                     /translation="MDFFKTIRVYSSFAKGTFKALSSYKANLLMGLIGQLIMISVTYF
                     LWIAIYSSSQEGIMNGFTLKEMLTYVMITLLIGIVTSNDISQEISFEVRDGSISTNLI
                     KPINYRLRIIFIGIGNFIFFFLTLFLPGAILISTYAILNGININLASILIFLISIILG
                     CLINIYYSYIFGLLSFKFYNIWGISQIARAIIMLVSGAMIPLTFFPEIVQKLFNFLPF
                     SSIIYTPAMIYLNKLSCSQIAKSLGLQLIWVVILMVLSKIMWNKVIDKLTIQGG"
     gene            complement(2936..3961)
                     /locus_tag="K8M77_002191"
     CDS             complement(2936..3961)
                     /locus_tag="K8M77_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI7086654.1"
                     /translation="MIKVKNLTKEFKIRKKESGLKGAFKSLFSSEFDSKTAVSNISFE
                     IKSGEIVGYIGSNGAGKSTTIKMMTGILTPSSGQVLVNGRVPYKEREKNAKDIGVVFG
                     QRTQLWWDLALSETFTILKDIYSIPDEVYEKNMKFLNKVLDLEEFMTRPVRTLSLGQR
                     MRGDLAASLIHNPKILYLDEPTIGLDVVVKEKVRTAIKEINKEFGTTVILTTHDLSDI
                     EELCKRIIIIDKGTKIYDGSLDDIKKKYGYMTTLEIQSKKDFKTLLNIKEILKSESNI
                     PIETFSSDDLSEYIESGKLIITFNKNKITSSKIISKVIERVDVLDFLLKESSIEDIIK
                     KIYRNEV"
     gene            4272..4940
                     /locus_tag="K8M77_002192"
     CDS             4272..4940
                     /locus_tag="K8M77_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBI7086655.1"
                     /translation="MKLDRKDIIDIFNFRFATKEFTGEIIPKEDMEMIAETARLSPSS
                     FGLEPWKFLIVENKELIKEISEVSWGFQRQASTTSHIVIALTKAGSEVKYDSDYIRNL
                     WINTKGVSEEFFEGIKDVLKNFQVGKLEADETNEKLLEWSKRQTYIALGNMMTAAAMR
                     EIDSCAIEGFDKEKVEEILAKKGLLDKEKYELTYLIAFGYRKENPNRKKSRLPKSEVI
                     QWIE"
BASE COUNT         1929 a          651 c          736 g         1730 t
ORIGIN      
        1 cgaatttacg ccctctgaga gttatatcaa aggaaagtag aatcctattc atagttttca
       61 aaatctgact cgtagaacag ggaattaaac aagatttata gttatttata gatttttggc
      121 aacggtgatt aacatgtggt ttaagaaatt atatgtagga actatagatt tattatcagc
      181 tattgtggaa aagatagcta aatgcaattt agcatgtaaa atttataaag cagtctttca
      241 attaggagtt tttccaaagc ctaagaactt taatgagata gttagtaatt tagtaataga
      301 gaaagatata atttatgaat catcaatttt agaggggaat aagttagatg tatattatcc
      361 taagaatctt aataaaaagg cgccaatatt aatgtggata catggtgggg gatatatagc
      421 taatagtaag gagactgtta agaattatat gatgactttg gctaataaag gctttgttgt
      481 gtttaatata gattatgcct tagctccaaa gtataaatat ccaagtcaaa tagttcaatg
      541 taatgaagct ttaaaatatg tctttgaaaa tgcagaaaag tttaatgggg atagagagaa
      601 tatttttatt ggaggagatt ctgctggggc tcaaatggct agtcagttag ttgcaattat
      661 aagcaatgag gaactatcta ataaaatgaa tttaaaacca agtataacaa ataaattttt
      721 aaggggagta atattatttt gtggattata taacatggat tctttaaggg aaactaaatt
      781 tccaggtata aaaacttata tggaagcatt aactggagag agtgattttg aaaattatga
      841 aagaatagat gaattatcta ctattaaata tataactcct aattatccaa gtacttttat
      901 aacggttgga gatattgatc cttttgtaga tcaaggaaaa gagttagcat cttctttaag
      961 agctttagga gtaaatgtcg tttcaagatt ttttgaaaat aaaggccttt ggcatgaata
     1021 tcaattcaaa ttttcaattc cagaatcaag ggaaaccttt aaaagtataa ttaaattttt
     1081 aaatgataat agtaaaagta gtaactgatt ttaattttaa aatagaatta aaattcactt
     1141 agaacatgga taaaaatgga ggcttatctt gttagcacat atcatgcttt aaggtaagcc
     1201 tctttttatt atataaatac taaaagataa ttttattgaa agttactttt atgtttattt
     1261 aagcatcata ttagaaataa ctaatttata tagtagttta tactatagct tagtatcttt
     1321 agtttccaga actttcgtaa gatgaaattc ctttaagcca tattctatat gatacaacta
     1381 ttgataaaat acctactatt aaggtaagta taactcccat gaagaagctt tccttaccaa
     1441 gtagatatgt tccagggtag tatcctgtaa aagcaaatgg aattataaag gttaataaga
     1501 attttatagc accagggtaa atacttatag gatacttaga aaaatcactt aatccatagg
     1561 ccatttgaac atgtctaaag cttgtcttag tccaaaaggc tatggaggct gtagcaagct
     1621 taactcctgt atagattaag gttcctccaa taacagcaat tataaagcct ataatccaaa
     1681 ggagagtaat atttacacta agctttattg cagaataaac cacaagaata aatccaacta
     1741 caagttctcc aaaaccatca ggttgaaatc tttcagctat tacttgaaat agtggattta
     1801 aaggccttaa taaatatcta tcaaactctc cacgaactat gcttctacca gaaaaaatcc
     1861 acaaataatc agtaaatatg tgatcaattc cccttggaat ttgagaaaat ccatatataa
     1921 agattatttc gtaaaaactc catccagaaa gagtgggtac attattaaat ataagagaaa
     1981 taaaaattat agaggtagct tgaactagaa aaaatcctaa aataccatat ataaaatcct
     2041 ctttgtattc aagcaatgtc tttaggtatt gcttcaaaaa ttgcacatat attctagcgt
     2101 atcttttcat aattctaacc tccttgaata gttaatttat caattacttt attccacata
     2161 attttagata ataccataag aattactacc catataagtt gcaatccaag ggatttagct
     2221 atttgagagc aacttaattt attaagataa atcatagctg gagtatatat aattgaacta
     2281 aaaggcaaaa agttaaataa cttttgaact atttcaggga agaatgtaag ggggatcatt
     2341 gcccctgaaa caagcattat aatagctcta gcaatttgac ttattcccca tatattataa
     2401 aacttaaaag agagtagtcc aaaaatataa gagtaataaa tatttattaa acatcctaaa
     2461 ataatactaa ttaaaaagat taatattgaa gctaagttaa tatttattcc atttaatatt
     2521 gcgtatgtgc taattaaaat tgcaccaggc aaaaatagtg ttaaaaagaa aaatataaag
     2581 tttcctatac ctataaatat tattctaagt ctataattta taggcttaat cagatttgta
     2641 gaaattgatc catctctaac ttcaaaggat atctcttggc ttatatcatt actggtaacg
     2701 attccgatta aaagtgtaat cataacataa gtaagcattt cctttagggt aaatccattc
     2761 ataattcctt cttgagagct actgtaaata gctatccaaa gaaaataagt aacacttatc
     2821 ataattaatt gtcctattag ccccataagt agattggctt tataagatga aagagcttta
     2881 aaagtaccct tagcaaagga actataaact cgtattgttt taaagaaatc catagctaca
     2941 cctcatttct atagattttc ttaataatat cttcaatgct actttctttt aataagaaat
     3001 ctaaaacatc aactctttca ataacctttg aaataatttt agaagatgtt attttatttt
     3061 tattaaaggt tataataagc tttccagatt caatatattc acttaaatca tcactagaga
     3121 aggtttcgat aggtatatta ctttcagatt ttaaaatttc cttaatgttt aataaggttt
     3181 taaaatcttt tttactttga atttctaaag tagtcatata accatatttt ttcttaatgt
     3241 catctaagct tccatcatat atcttagtac ctttgtctat gataattatt cttttacata
     3301 attcctcaat atcacttaaa tcgtgagtag ttagtatgac agttgttcca aattctttat
     3361 ttatttcttt tatggctgtc cttacttttt cttttacaac tacatcaagt ccaatggtag
     3421 gttcgtctag atatagtatt ttaggattat ggattaaaga ggcagctaaa tctcctctca
     3481 tcctttgtcc aagagaaaga gttctaacag gtcttgtcat gaattcttct aaatctaaaa
     3541 ccttatttaa aaatttcatg tttttttcat atacttcatc tggaatagag taaatgtctt
     3601 ttaatattgt aaatgtctca gatagagcta agtcccacca aagttgagtt ctttgaccaa
     3661 aaacaacacc aatatcttta gcattttttt ctctttcctt atagggaact cgtccattta
     3721 ctaatacctg accagaggat ggggttagta ttcctgtcat cattttaatg gttgtggatt
     3781 taccagcccc attagagcct atataaccta cgatttcccc gcttttaatt tcaaaggaaa
     3841 tatttgatac tgctgttttt gaatcaaact cactagaaaa aagagattta aaggctcctt
     3901 taagtccact ttctttttta cgaatcttaa attccttagt taaattttta acttttatca
     3961 aaaaaacacc tcctaaaaat ttctcaataa ttccccttag catattgatt aaaagtaaaa
     4021 tatttttctt ttgaatacga ttaaccaata atacttatta tattttagtt ctttttcaat
     4081 aaaattacaa ttggaattta aagaaattta aatagaaata cactatgatt tagatattat
     4141 atttttaatt atatacctga gtaaatagat aagaattgtg gtataataaa taaaataata
     4201 atatcttaat aattagaaaa taataatatt aaataaaaat aataaatcaa catgaatagg
     4261 aggatataat aatgaaattg gatagaaaag atattataga tatttttaat tttagatttg
     4321 ctacaaagga atttacaggg gagataatac ctaaagagga tatggagatg attgcagaaa
     4381 cagctagatt atctccaagt tcatttggct tagagccttg gaagtttctt attgtagaaa
     4441 ataaagaatt aattaaagaa atttcagaag tatcttgggg atttcaaaga caagcttcaa
     4501 caacaagtca tattgtaatt gctttaacaa aggcaggaag tgaagttaaa tatgattcag
     4561 attatattag aaatctttgg attaatacaa aaggggtttc agaagaattt tttgaaggaa
     4621 taaaagatgt attaaagaac ttccaagttg gaaagttaga agctgatgaa acaaatgaaa
     4681 aattacttga atggtcaaaa agacaaactt atatagcatt agggaatatg atgacagcag
     4741 cggctatgag agaaatagat tcatgtgcta ttgagggatt tgataaagaa aaggtagaag
     4801 aaatattagc taaaaaaggg cttttagata aagaaaaata tgaattaaca tatttaatag
     4861 catttggata tagaaaagag aatcctaata gaaagaaatc aaggcttcca aaaagtgaag
     4921 taattcaatg gatagaataa agtatataat tttctaaatt aaaatgagct gtatcaatat
     4981 ataacaataa aagatatttt gatacatgct ctttttttat gaaataaatt ttcaaaaaca
     5041 aaaatt
//
LOCUS       DAEVGI010000157         5019 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEVGI010000157 DAEVGI010000000
VERSION     DAEVGI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.157"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(127..2025)
                     /locus_tag="K8M77_002193"
     CDS             complement(127..2025)
                     /locus_tag="K8M77_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7086656.1"
                     /translation="MDKTIIKQLFKINKTPFPWQKAINAGICAGFPVLIGVLIGQIRL
                     GLLGGIGSFSYLYVINEPYAQRGKKILFAAIGISLSVALGTLLAPYPILIILMVGLIG
                     GITIFIFGLLKIPGPSSMFFVLSFLVSTGMPVNQSEAFIRFFVVLMGGLFAWLMSMKG
                     YFLNPHGPEIKVIKRLYLTLADFSEAIGNEDINDIRNKTVNALREAEEILLTGYIPWK
                     NSFLFNRLYLLNEEGNKLFLEMLELHVNKNKKLPKEFSNMIRKLAMAIDFKDGDKLKI
                     DSYENLDMDYHKLLQIIYDTEAIINLPLTYIGRSIKISKTSLMSKVKRACNKDSIVFI
                     NAIRYGVVLSISAIIAFSFPFSRPYWIPLSCAAVMSGSTIISTFHKSIQRSFGTIIGV
                     IIATIILKTNPNGFIIVIINMILTALTELFIVKNYAIAVMFITPNALLIAETSTQIHN
                     ITYFATARITDIVVGSLIGLIGIYIFGRRSASSRVKDLIVKLIHSQKNLLVLLSTNDK
                     EINNKKIKNMKEKMGVDLMNLKMAYNTALGEVPRNEEMLEMLWPVFFSLEHISYLLDK
                     YCTTKKYLNLSDDELAQLLLVLEKIAIFIEQGIDVKPKKVPNIKEISQICEEFNSIQR
                     VFYIKSHK"
     gene            complement(2073..2480)
                     /locus_tag="K8M77_002194"
     CDS             complement(2073..2480)
                     /locus_tag="K8M77_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI7086657.1"
                     /translation="MNLYGHKVNQLARNFTKKLNEKIAPLGLYSSQWGIILYLHEKKQ
                     CTQIEISQYLGVEAPTITRTLTRMEKMGLVIRTEGKDKRERIINLTEKAYEMFPKWFE
                     EAKNIEVEAINNINKEELEIFNNVLEKMMNNLD"
     gene            complement(2618..3715)
                     /locus_tag="K8M77_002195"
     CDS             complement(2618..3715)
                     /locus_tag="K8M77_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBI7086658.1"
                     /translation="MENTWEKKWVEDIEYLKEELKKRHKNLFAYTAEESFNEKVENLK
                     NIVNELDYEEMKVEISRVVSSLRDAHTSLIFPAKRFIPLKFYYFNDGVYIINTCKGYE
                     KLLFKKVLALGDMKIDEVLEELSNIISFENEYFFKAQSMKYMQIAEVLYGLLIIDTMD
                     KVKITLDEGEYEVLTCSFEDLVYANKRLPMYAKNDFSNLWFEVLKSGELYIKYNSCRE
                     QGEESIGKKIENIICLIEKENIEKVTVDLRNNLGGDSTLFTPLIDYLKSSEKINKKEN
                     LKVIIGRETFSSALLNAYTFKNSTNAKIIGEPSGGKPNCYGEILRLTLPNSKLVITYS
                     TRFYKLIEDDLVMALYPEEILLESIEDYINV"
     gene            4181..4633
                     /locus_tag="K8M77_002196"
     CDS             4181..4633
                     /locus_tag="K8M77_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBI7086659.1"
                     /translation="MDVLVYVAISVGVLSGVWAWMAAVFGMVSWTGFVGWTSYYSSGG
                     MAKGLFRSLCSNATGIIYALLIIKGSSIFQNEYWSYFLTAFFSFMMVIQAKLKKFEFI
                     SGAFCGCFSTFGLNGNWQAVIPALLCGNVLGYISQKIGIYCHKVFSKN"
     gene            complement(4738..>5019)
                     /locus_tag="K8M77_002197"
     CDS             complement(4738..>5019)
                     /locus_tag="K8M77_002197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086660.1"
                     /translation="KITKFIAGGIEVLLGIPVLGASIILGLSWIPLGVMLVFHIINLV
                     LSKNENLPITGSILGVVGNALGWIPFLGMIMHILTAVFVIIEACKMKVE"
BASE COUNT         1739 a          852 c          548 g         1880 t
ORIGIN      
        1 ttatactgta tgaatacgtt tcaagcatca aaatacgaat ttttatgcat tgaaaaattt
       61 ttataaaaaa tattcaacct taaatttaat taaaaagtta aggttgaaca caataaataa
      121 actctattat ttatgagatt taatataaaa taccctttgt atagaattaa attcttcgca
      181 aatttgagat atttccttta tattaggaac ctttttaggt tttacatcta ttccctgctc
      241 aataaatatt gctattttct ctaatacaag taataattgg gctaattcat catcagataa
      301 atttaaatat ttcttagttg tacaatactt atctagtaag taactaatat gctctaaaga
      361 aaagaataca ggccatagca tttcaagcat ttcctcatta cgaggaactt cccctaaggc
      421 agtgttatat gccatcttaa gattcattaa gtctacaccc attttttctt tcatattttt
      481 tatcttttta ttattaattt ctttatcatt agttgatagg agaactaata aattcttttg
      541 actatgtatt aattttacta ttaaatcctt tactctacta gaagctgagc gtcgtccaaa
      601 aatataaata cctattaaac ctattaaaga acctaccact atatcagtaa tacgtgctgt
      661 tgcaaaataa gtaatattat gtatttgtgt tgaagtctct gctataagta aagcatttgg
      721 cgtaataaac attactgcta tggcataatt ttttacaata aaaagttctg ttaaagctgt
      781 taaaatcata ttaattataa ctattataaa accatttgga tttgttttta aaataatagt
      841 agctattatt actccaataa ttgtaccaaa ggatctttga attgatttat gaaaggttga
      901 tataatagtt gaacctgaca tgacagcagc acaggataaa ggtatccagt atggccttga
      961 aaaaggaaaa ctaaaggcta ttatagctga tatagaaaga acaaccccat atctaattgc
     1021 attaataaat actattgaat ctttattaca agctctttta accttactca ttaatgaagt
     1081 ttttgaaatt ttaatgcttc ttccaatgta tgttaatggc aaattaatta tagcttcagt
     1141 atcataaatt atttgtaaaa gcttatggta atccatatct aagttttcat aagaatctat
     1201 cttaagcttg tctccatcct taaagtcaat tgccatagct aactttctta tcatattact
     1261 aaattctttt ggtaatttct tatttttatt aacatgcaac tcaagcattt ctaaaaataa
     1321 tttattaccc tcttcattta aaaggtataa tctattaaat aagaatgaat tcttccatgg
     1381 aatatatcca gttaaaagaa tttcctctgc ttcccttaaa gcatttacag ttttatttct
     1441 tatatcatta atatcttcat ttccaatggc ttcactaaaa tctgctaagg ttaaataaag
     1501 tcttttaata acttttattt caggaccatg tggatttaaa aaatatccct tcatgctcat
     1561 taaccatgca aaaagccctc ccataagaac aacaaaaaat cttataaacg cttctgactg
     1621 attaacaggc attcctgttg atactaaaaa acttaataca aagaacattg aagaaggtcc
     1681 tggtattttt aaaagaccaa atataaaaat tgtaattcct ccaattaaac ctaccattaa
     1741 tataataagt attgggtagg gagctaataa agttcctaaa gcaactgaca agctaatacc
     1801 aatggctgca aacaatattt ttttaccacg ctgagcataa ggctcattta taacatataa
     1861 atatgaaaaa cttcctattc cacctaataa tcctaaacga atttgaccta ttagaactcc
     1921 tataagtact ggaaatcctg cacagattcc agcatttatt gctttttgcc aaggaaaagg
     1981 agttttatta attttaaata attgctttat tatagtttta tccatgtaca ctccttttta
     2041 aagtctatat tttctactaa actttatatt tattaatcta aattgttcat cattttctct
     2101 aaaacattat taaaaatctc taattcttct ttattaatat tatttatagc ctctacttct
     2161 atattcttgg cttcttcaaa ccatttagga aacatttcat aagccttttc tgttaaattt
     2221 ataattcttt cacgcttatc cttcccttca gttctaataa ctaagcccat tttctccatc
     2281 ctagttaagg ttctagtaat tgttggagcc tctaccccaa gatattgact tatttctatt
     2341 tgggtacact gtttcttttc atgtaaatat aatataatac cccattgtga tgaatatagc
     2401 ccaagaggag caatcttctc atttaatttc ttagtaaaat ttctagctag ttgattcact
     2461 ttatgtccat ataagttcaa ttttattcct cctttcattt aattacctaa gtaataatta
     2521 cctaggtaat tatatcactt taatttataa gaatcaaatt cccataaaaa aaccacatga
     2581 atatatttat ataacatatt catgtggtta tgaaaaatta tacatttata taatcttcta
     2641 ttgactctaa aagaatctcc tctgggtaaa gagccataac taaatcatct tctataagct
     2701 tataaaatct tgtagaataa gtaattacaa gcttactatt tggaagagta agccttagga
     2761 tttccccata acaattaggc tttcctccac taggttctcc tattattttt gcattagtac
     2821 tatttttaaa ggtataagca tttaaaaggg ctgaagaaaa agtttctctt cctattataa
     2881 cttttaaatt ttccttttta tttatctttt cactgctctt taaataatct attaaaggag
     2941 taaaaagtgt tgaatctccc cctaaattat ttcttaaatc tacagttacc ttttctatat
     3001 tttccttctc aattaggcat attatatttt caatcttttt ccctatactt tcctccccct
     3061 gttccctaca gctattatat tttatgtata actctccact ttttaaaact tcaaaccaaa
     3121 gattagaaaa atcattttta gcatacatag gcagtctttt atttgcataa actaaatcct
     3181 caaaggaaca ggttaaaacc tcatattctc cctcatctaa ggttattttt actttgtcca
     3241 tggtatcaat tattaaaagt ccatataaaa cttctgctat ttgcatatat ttcatgcttt
     3301 gagccttaaa gaaatactca ttctcaaagg aaataatatt agaaagttcc tctaaaacct
     3361 catctatctt catatcacca agggctaaaa ccttcttaaa taagagcttt tcataccctt
     3421 tgcatgtatt tataatatat actccatcat taaaataata aaactttaat ggaataaacc
     3481 ttttagcagg aaatataagt gaagtatgag catctcttaa agaagacaca acccttgata
     3541 tttcaacctt catctcttca taatctagtt cattaacaat gttttttaaa ttctctacct
     3601 tttcattgaa actttcttcc gctgtataag caaataaatt tttatgcctt ttcttaagct
     3661 cttcttttaa atactcaata tcctctaccc actttttctc ccatgtattt tccataagct
     3721 ctcctttaca gataatatta aaatactaat actataaatt tatgtttcaa atatatatag
     3781 gttattttaa atttaccttt aactttttta tttatctctt aatctcttta aaactacttc
     3841 tctttctaaa tcctctaaac taaaatccac ttaaatcctc taagacacaa tctacgtctc
     3901 cttttcaaaa tatcttaaga cctaatctat atctcttcta taaacacctt aagaaaaaat
     3961 ctatatataa aatatatact aagttaaaat ccacgtcctc tctttttaga aatatcttaa
     4021 actaaaacct atttctactt tagaaaacat ctattgtaaa ctatttatat tttaacacat
     4081 taataatttg attaaaggtt tacttttgga taaaatatta atgtacgata aaaacattaa
     4141 tattaagttt ataagatact aatgaggagg atatttttct atggacgtac ttgtatatgt
     4201 ggctatcagc gttggtgtac tatctggagt ttgggcatgg atggcagcag tatttggaat
     4261 ggtaagttgg actggctttg ttggttggac tagttattat tcctcaggtg gaatggctaa
     4321 aggacttttt agatctcttt gctcaaatgc aacaggaatt atttacgccc tgcttattat
     4381 aaaaggatca tctattttcc aaaatgaata ttggtcttat tttttaacag catttttctc
     4441 ttttatgatg gtaatacaag ccaagcttaa aaaattcgaa tttatctctg gagcattctg
     4501 tggctgcttc tcaacctttg gattaaacgg aaattggcaa gctgtaatac cagcactact
     4561 ttgtggaaac gtattaggat atatatcaca aaagatagga atctactgtc ataaagtatt
     4621 tagtaaaaat taaataaaaa acaaagacaa gtaataaaat aaatccttgt ctttgtttta
     4681 ttctacaatt acaaatttaa cggaattaat acatggtatt ttaaaattcc aattaattta
     4741 ttctactttc attttacaag cttctatgat tacaaagaca gcagttaata tatgcataat
     4801 cattcctaag aaaggtatcc atcccagggc attaccaact acccctaaaa tactgcctgt
     4861 aataggtaaa ttttcatttt tagataaaac taaattaatg atatgaaata ctagcataac
     4921 ccctaaagga atccatgata acccaagaat tatacttgct cccaatacag gaataccaag
     4981 caatacttca atacctcctg caataaattt ggtaatttt
//
LOCUS       DAEVGI010000158         4984 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEVGI010000158 DAEVGI010000000
VERSION     DAEVGI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.158"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(46..459)
                     /locus_tag="K8M77_002198"
     CDS             complement(46..459)
                     /locus_tag="K8M77_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086661.1"
                     /translation="MYVAFGNRVLDSEEIKKEIELNTDARVVSDLCKSSKREDIIAFK
                     LSIDMDILRGLMRENSDLKDLNDEELFEDYLDLAEEVAGMIFEYMPEDAILDIRSYKW
                     DMSYNDVNLIMVMAHEDLGIAKVNDVMKRLLRQVD"
     gene            complement(668..1309)
                     /locus_tag="K8M77_002199"
     CDS             complement(668..1309)
                     /locus_tag="K8M77_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HBI7086662.1"
                     /translation="MSILSIVLTGFGLAMDAFAVSVAKGITLTRVKAKDALKVALFFG
                     GFQALMPLIGWGAGRYFADYIKAFDHWIAFILLSFIGGKMIFEALKEEDEEKAEVAVS
                     MEVNKNKEREFANMKRKEELSAKNLTVLAIATSIDALAVGVSFAFLGISIVQTIIIIG
                     IITFVLCFLGVIIGEKLGDIFKNYAEIVGGVILILIGINILLEHTGIIEKLFS"
     gene            complement(1664..2194)
                     /locus_tag="K8M77_002200"
     CDS             complement(1664..2194)
                     /locus_tag="K8M77_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086663.1"
                     /translation="MNKRKIVASILTTSLLVTSLVGCVGSNNKANTNSNDSKVQESVE
                     NQSDFNDLRTYVGKTYNEVSENKGTGNENIEEVSGKKVIVSSSYSTRMFNYNADLILE
                     LDDSKNISAVSVHFKGIEPNNILENIKKILGEPKISEDKENGDSKVYSWEKDGYQYKL
                     SQVGEETIINVNKSAI"
     gene            complement(2346..2525)
                     /locus_tag="K8M77_002201"
     CDS             complement(2346..2525)
                     /locus_tag="K8M77_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086664.1"
                     /translation="MEYNKHRYLGFLGLFSLVGLKGFTGGNALWYIFFLNFLWFTLFY
                     ENSWLYVGSPFKFNK"
     gene            complement(2606..3274)
                     /locus_tag="K8M77_002202"
     CDS             complement(2606..3274)
                     /locus_tag="K8M77_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBI7086665.1"
                     /translation="MEKKILIVEDDPMVALINKRFLENMGFKDILGPVQTEEEIIQLL
                     DKENIDLILLDVYLPKKNGIDILKSLRYKKYLTDVIMITAANSVEEVKRAFAYGVTDY
                     LVKPFEFERFEEAINKYKQKNNLLNKREALSQQDIDVISKSLEEKIELPKGLNQKTLD
                     RIMEFLKENKGKVWTLREIAYELKISNVTIKKYMDYLEDIKKVNVTLTSGNVGRPEYK
                     YTLG"
     gene            complement(3274..4824)
                     /locus_tag="K8M77_002203"
     CDS             complement(3274..4824)
                     /locus_tag="K8M77_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7086666.1"
                     /translation="MKVKFENKILLWAILITFIPLALSFTLFIEDKLSYMDEDVRNTL
                     KETAFSISQIPFVQEDLNNGEINPRIQEYTKHFIEAINDVDIIVVADMRGIKYSHLDE
                     KQIGQVFINDDKKEVLTQGNSYYSLMKGSMGETLRWFQPVMYNGKQVGFIMVGKYYNE
                     IQLLTHKTLIKYMGLFILVFLITILISKLFARKVKKAILGMEPEEIAALYKEKKIILN
                     TVSEGIIALNKNNEITEINDNCYKLIDGFSKYKVLKKLLPYIEENKPVEMKEIILQGK
                     KVFVTIQPIMKKGEYLGSVITLMDRNDIRKIAKEITGVDEVVKNLRANVHEFRNNLHV
                     ILGLIQLGEYEEARKYILKTQKIHENNSLKFSNVEDYYVRGLLLSRELVAKERGVNFV
                     LTEESFLFGNHNYVDSYDIVTILGNLIENAFDSCVCSSIENKEVEVTLYEDDEKIELQ
                     VRDNGKPIDNNIKERIFELGVSSKGEGRGTGLSLVKNRVELYDGCIDIEEFNEEKIFI
                     ITILKGEK"
BASE COUNT         1682 a          918 c          398 g         1986 t
ORIGIN      
        1 ttattttata aaaattaatt tgaaagttta tttcatcatt taaaattaat ctacttgtct
       61 taataatctc ttcataacat cattaacctt agctattcca agatcttcat gagccattac
      121 cattattaaa tttacatcat tgtaagacat atcccattta tatgatctta tatctaatat
      181 agcatcttct ggcatgtatt caaatatcat tccagcaacc tcttctgcta aatccaaata
      241 atcttcaaat aactcttcat catttaaatc tttaagatca gaattttctc tcataagacc
      301 tcttaagata tccatatcta tgcttagttt aaaagcaatt atatcttctc tttttgagct
      361 cttacataaa tcacttacaa ctctggcatc agtattaagt tctatttctt tttttatttc
      421 ttcactatct aacactctgt ttccaaatgc aacgtacatg taaattacct ccttagttgt
      481 ttataactct aaaccatatt taacacaaat atatttaatt aaaattatta ttctaaaatc
      541 tacctatata tttatatcac ttgttcatat attattacaa gtaatatttt ttctcttgtt
      601 taaaaaatag tgattttaag tttcaaccta aaataaagtc atatctatcc ttaccttaat
      661 tatgatacta agaaaataac ttttcaataa ttcctgtatg ttctaataat atatttatac
      721 ctataagaat taatataact ccacctacta tttcagcata gtttttaaat atatctccta
      781 atttttcacc tattattacc cctaaaaaac ataaaacaaa agttataata ccaattatta
      841 ttatagtttg aactatagaa atacctaaga atgcaaaact aaccccaact gctaaggcat
      901 caatactagt tgcaatagct aatacagtaa gatttttagc acttaactcc tctttgcgtt
      961 tcatattagc aaattctctc tctttatttt tgttaacttc cattgatact gcaacctcag
     1021 ctttttcttc atcctcttct tttaaagctt caaaaatcat ttttccccct atgaaactta
     1081 ataatataaa tgctatccaa tgatcaaaag cttttatata atcagcgaaa tatctcccag
     1141 ctccccatcc tattaaaggc ataagtgctt gaaatcctcc aaagaataaa gcaactttta
     1201 atgcatcttt agcttttact ctagttaggg ttattccctt agcaactgat acggcaaaag
     1261 catccatagc aaggccaaaa ccagttaaaa caatagataa aatactcata atacatcctc
     1321 ctacttataa aaactttata aaaaaagacc tatatgcagc aaaaaactgc atataagtct
     1381 cgctatttaa gattaagcca gaccaaagcc ctttggtcaa tttgttgact taatcactca
     1441 ttcaacttga gctaactact ccctcatatg gaaacaaagt ttgttaaata ctttaaaact
     1501 ttatcataaa tatttgcata aatcaatggt atattactat taaagtttat taacataaat
     1561 attctcttta tattttactc aaatttcatt aaatttattt cactaatttt gcataaacta
     1621 tttttaaatt attatcttta aagtattata aaatttaaat aaattaaata gcactcttat
     1681 ttacattgat tatagtttct tctccaactt gtgaaagctt atattgatat ccatcttttt
     1741 cccatgaata aactttagaa tcaccatttt ccttatcctc gctaattttt ggctcaccca
     1801 atatcttctt tatattttct aatatattat ttggctctat tcctttaaag tgaacactta
     1861 ctgcacttat attttttgaa tcatcaagtt ctagtattaa atctgcatta taattaaaca
     1921 ttcttgtaga ataacttgag gaaacaataa ctttttttcc tgaaacttct tctatatttt
     1981 catttccagt tcctttattt tctgaaacct cattgtaagt ctttcctaca taagttctta
     2041 aatcattaaa atcactttga ttttcaacac tttcttgaac ttttgaatca ttactatttg
     2101 tattggcctt attatttgat ccaacacacc caactagaga tgtaactaat aaagaggtcg
     2161 ttaaaatact agcaacaatc tttcttttat tcattttttc tcccacctat ttttcatgtg
     2221 ttttttatac aaatgtatat tacttgtata ttcatctaat tatagtatag tttccccaag
     2281 gaaaaattac aaaacttatt tttaagctct tttaattaag aaaatatact ctaaacaaaa
     2341 atattttatt tattaaactt aaatggtgat cctacatata accaactgtt ctcataaaaa
     2401 agtgtaaacc ataagaaatt aagaaagaaa atataccaca gggcatttcc tcccgtaaat
     2461 cccttaagtc caactaatga aaacaaccct aaaaatccta aatatctatg cttgttatat
     2521 tccatattat cccctcctaa taagtatttc taatataatt atactataca aatcaggaaa
     2581 tattttctat gaaaatcttt atttattaac ctagagtgta tttatattca ggtcttccaa
     2641 catttccact agttagtgtt acatttacct ttttaatatc ttctaagtaa tccatatatt
     2701 tttttattgt tacattgctt atttttaatt cataggctat ttctcttagt gtccaaacct
     2761 ttcctttatt ttcttttaaa aactccatta ttctatctaa agttttttga tttaatccct
     2821 taggtaattc aatcttttcc tctaaagatt ttgaaataac atctatatcc tgctgactta
     2881 aagcctctct cttatttaaa agattatttt tttgtttata cttatttata gcctcctcaa
     2941 acctttcaaa ttcaaaaggc ttaactaagt aatcagttac cccataagca aaggctctct
     3001 tcacctcttc aacactattt gcagcagtta tcattattac atcagttaaa tacttcttgt
     3061 accttaaact ttttaatata tcaataccat ttttcttagg aagatataca tctaaaagaa
     3121 ttaaatcaat attttcttta tctaatagct gtattatttc ctcctcagtt tgtactggtc
     3181 ctaatatatc tttaaagccc atattttcta aaaatctttt attaataaga gccaccattg
     3241 gatcatcttc tactataagt atcttctttt ccattatttt tctcctttta aaatagttat
     3301 aataaatatt ttctcttcat tgaattcctc aatatcaata caaccatcgt aaagctcaac
     3361 tctattctta actaatgata atcctgttcc tcttccttct ccctttgagg aaactcctaa
     3421 ttcaaatatt ctttccttta tattattatc tataggtttc ccattatccc taacttgcaa
     3481 ttctatcttt tcatcatctt catataaagt aacctcaact tccttgttct caatacttga
     3541 acatacacaa gaatcaaaag cattttctat taagttacct aaaatagtta caatatcata
     3601 ggaatcaaca taattatgat ttccaaataa aaatgattcc tctgttaaaa caaagtttac
     3661 tcctctttct ttagcaacta actctctact taaaagtaat cctcttacat aataatcctc
     3721 tacatttgaa aattttaaag agttattttc atgaatcttt tgagttttta atatgtattt
     3781 tctagcctct tcatattcac ctagttgaat taaccctaaa attacatgta gattatttct
     3841 aaattcatgg acattagctc ttagattctt aacaacctcg tcaacaccag ttatctcttt
     3901 tgcaattttt ctaatatcat ttctatccat taaagttatt acagaaccta aatattcacc
     3961 ctttttcata ataggttgta tagttacaaa taccttcttc ccctgaagta taatttcttt
     4021 catttctaca ggtttatttt cttctatata aggtaataat tttttcaaca ccttatactt
     4081 tgaaaatcca tctataagct tatagcaatt atcatttatc tctgttattt cattattttt
     4141 atttaaggca ataatacctt cactcactgt atttaatatt atcttttttt ctttatataa
     4201 agcagcaatt tcttctggtt ccattcccaa aattgccttt ttaacctttc tagcaaaaag
     4261 tttagatatt aatatagtaa ttaaaaaaac taaaataaaa agtcccatat attttattaa
     4321 agtcttatga gttagaagtt gaatctcatt ataatactta ccaaccataa taaacccaac
     4381 ttgctttcca ttgtacatta caggttgaaa ccatcttaat gtttccccca tagaaccctt
     4441 catcaaagaa taataactgt ttccttgtgt taaaacttct ttcttatcat cattaataaa
     4501 gacctgccct atttgctttt catctaaatg agaatactta atccccctca tatcagcaac
     4561 aacaataata tcaacatcat ttatagcttc aataaaatgt tttgtgtatt cttgtattct
     4621 agggtttatt tctccattat taagatcctc ctgaacaaaa ggaatctgac ttatactaaa
     4681 agcagtctct tttaaggtat ttcttacatc ctcatccata tatgaaagct tatcttctat
     4741 aaataaagta aaagataatg ctagtggaat gaaagttata agtatagccc ataataatat
     4801 tttattttca aattttactt tcatattttt caccaccgtg aaggaattat atcatattgt
     4861 aaagattact ccaattctat ttttaatttt tcattaattt tttctaaatc catattttta
     4921 acttttttct tttttaaata tattgtttag ttttaaatta agcttagaaa atcttataaa
     4981 aaaa
//
LOCUS       DAEVGI010000159         4854 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEVGI010000159 DAEVGI010000000
VERSION     DAEVGI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.160"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            80..1051
                     /locus_tag="K8M77_002204"
     CDS             80..1051
                     /locus_tag="K8M77_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBI7086667.1"
                     /translation="MQISNVDIKLNSDDLMSFINDFVKIKGLEIKSLKINNGIEVEGV
                     FKKVLALGFKATLNILGVEGDNLVIEFSKAKLMSVGILKPFRKIGLKIALKGFKNQGI
                     LVKEDKILVGYKKILGNIPFLNLDFKELKIQGEVLNIGLEKIDVELSKIKEPVLEIEV
                     RDDIEEEEKTDDYIMSIDVQKVEDMYTSSRDYVVNHIPEKAKDYKDYIMFLPDIVALI
                     FRLLKDNRVPMKTKAIVGASLGYVVCPFDILPDKIPFVGALDDLSVIFFALNRMINDV
                     DINVILENWQGENEFVVILRKTVEFFSGFTGANNLDNIYEVIDSITN"
     gene            1068..1697
                     /locus_tag="K8M77_002205"
     CDS             1068..1697
                     /locus_tag="K8M77_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBI7086668.1"
                     /translation="MAKKYYAIAKGKSGIPKIVGTWPECQKEVIGCKGAKYKSFTSKD
                     EAEKFISIHENGGSFEEVKGNEEVKDDLIYIYVDGSFMVSKGNYSYGFLVVKNDEILY
                     EDNGIGYDKEAIALRNVSGEVEGAMKAIEYAIENGYKDIVLCYDYQGIESWALGTWKR
                     NNRITQNYNEFMQEKFKLIKVRFKKIKGHSGNKFNDRADILAKKALESL"
     gene            1776..1937
                     /locus_tag="K8M77_002206"
     CDS             1776..1937
                     /locus_tag="K8M77_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086669.1"
                     /translation="MAKKLMTTLAIGVIGAAVGMMVTPNLDRKTQRALKKASRRMIDA
                     AGESMGWME"
     gene            2233..3534
                     /locus_tag="K8M77_002207"
     CDS             2233..3534
                     /locus_tag="K8M77_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086670.1"
                     /translation="MEILSLGEKIKRRRKELNMTLKELAGERITPGQISLVESGRSNP
                     SMDLLEYIAITLNTSVEYLMETEKTQAEKICLYYEQIAESAILNDDIKKGEKFIEYAI
                     YYADKYDLIYRKAKNLALKAEMYLKSEEYLLAQQYFLSANILFLQINSNVDMVKTYLN
                     IAKISLHSKAYYSANSYLKEAEKIYINNKLTEDLLLGEIYYLISKTYFFIENIQKAIN
                     FAFLAKSEFEKVYNKKAYAKSLELLAEEHAKTGDLESAIAYSKQSLNVYKELEQLDKI
                     SSIEDSLGKLFFEFDNIEESFEHYNRAIKIREENTDSKLLDTLFNLCENHIKTKNMNK
                     CIEILNEIKNKLNVSDVNRLIEYKILTFRIEVLQNNIKEAENILLDTLEIAKRNNMKK
                     KEADISIRLGKFYLELKRDYEAAKYLDKGINNLKNLGVIKS"
     gene            3551..4816
                     /locus_tag="K8M77_002208"
     CDS             3551..4816
                     /locus_tag="K8M77_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086671.1"
                     /translation="MDFLSTGEKIKRARIYKGLTLKQLCEDDISISRMSCIENGKVKA
                     DKWVLELVAKRLDLDLEYLLCDDITQLNNSIDKYIQKKVLTESEADEIKEYLEYSLSK
                     EYEDISAKLMHILFINYTNIREFKKAKDLLNEYSNIYESNKESKRLYYEDLALYFTVR
                     KNFADAITYYNMLLDFLLKDGIEKNNEIYIKNATALAICNYRVGHIEKSREIIKDIFS
                     IQGIDLNSKCLGEAQGILAILSLLEGNSEEFEKNYTYYKEKIDCNSYNWKRINSLIID
                     AFLKCGKYEEAQKLTEESIEQVNKEDKIGYIELLLFIINVYNANGCIESVKEYCELSL
                     ELAINKNNMFFIEKGYYFKAELSKNERNDMQWEMYMNLATDLLLKFGTYEEKRERYLE
                     MADLYHYLDDTNEALKYLSFAIKEVDNLY"
BASE COUNT         2082 a          399 c          843 g         1530 t
ORIGIN      
        1 tttttttacc ttcaaaatta agaataaata ctatatacta aaagatggat aattattaaa
       61 taataagggg agaagtattt tgcaaatttc aaatgtagat ataaaattaa attcagatga
      121 cttaatgagc tttattaatg attttgtaaa aattaagggt ttagaaataa aaagcttaaa
      181 aataaacaat ggtatagaag tagaaggagt gtttaaaaag gttcttgctt taggttttaa
      241 agcaactttg aatattttag gagtagaagg agataatctt gttatagaat tttcaaaagc
      301 taaacttatg agtgttggaa ttttaaagcc ttttagaaaa ataggtctaa agatagcttt
      361 aaaaggtttt aaaaatcagg ggatcttagt taaggaggat aaaatacttg taggttataa
      421 gaaaatacta ggtaatatac ctttcttaaa tttagatttt aaggaattaa aaattcaagg
      481 agaggtttta aatattggat tagaaaaaat agatgtagag ctttcaaaga ttaaggaacc
      541 tgttttagaa atagaagtga gagatgacat tgaagaagag gaaaagacag atgattacat
      601 aatgagcatt gatgtacaaa aagttgagga tatgtataca agtagcagag attatgtggt
      661 taatcatatt ccagagaagg ctaaagatta taaggattat attatgttct taccagatat
      721 agtagcttta atatttagac ttttaaaaga taatagagtt cctatgaaga ctaaggctat
      781 agtaggtgct tctttaggat atgtagtttg tccatttgat atattaccag ataaaatacc
      841 ttttgtaggt gctttagatg atttaagtgt aattttcttt gctttaaata gaatgataaa
      901 tgatgtagac ataaatgtta tattagaaaa ttggcaagga gaaaatgaat ttgtagttat
      961 tttaagaaag actgtagaat tctttagtgg atttacaggt gctaataatt tagataatat
     1021 atatgaagtc atagattcaa taactaatta aaaaaaggag taaaataatg gctaaaaaat
     1081 attatgctat agcaaaagga aaatcaggaa ttcctaaaat tgttgggact tggccagaat
     1141 gccaaaaaga agtaatagga tgtaaaggcg ctaagtacaa aagtttcact tcaaaggacg
     1201 aggcagaaaa gtttatttct attcatgaaa atggaggaag ctttgaggaa gttaaaggaa
     1261 atgaagaggt taaggatgat ttaatttata tttacgtaga tggtagcttt atggtttcta
     1321 aaggaaatta ttcttatggc tttttagtag taaaaaatga tgagatatta tatgaagata
     1381 atggtattgg atatgataaa gaagcaatag ctctaagaaa cgtatcagga gaagttgaag
     1441 gagctatgaa agcaattgaa tatgccatag aaaatggata taaagatata gttctttgtt
     1501 atgattatca aggaattgaa agctgggctt taggaacttg gaaaagaaat aatagaataa
     1561 ctcaaaatta taatgaattt atgcaagaaa aatttaaact cataaaggtt agatttaaaa
     1621 aaataaaagg acatagtgga aataaattta atgatagagc agatatactt gccaaaaaag
     1681 cattagaaag tctttaatgc tatgaattac ctctaataaa aatattttaa ttagacaaac
     1741 taaatatgaa atcattatta aaagaggtga ttattatggc aaaaaaatta atgactactt
     1801 tagccatagg tgttattgga gcagctgttg gtatgatggt aactccaaat ttagacagaa
     1861 aaactcaaag agctcttaaa aaggcttcaa gaagaatgat tgatgcagcg ggcgaaagta
     1921 tgggatggat ggaataactt ataaaaaagc agcttaaggc tgcttttttg tatatatata
     1981 gaaatttttt tattatgtag agaaattttc aattataata tataatattt ttaaagaata
     2041 agtatatata ttaaaaaatt aaagatataa gttattaaaa ttaaagtata tgattaaaaa
     2101 tgttaaaaaa tgcacaaaca tatagatttt tttacaaaaa tactgaataa ttgacattaa
     2161 aacctaattt tgtgatataa tttaattggt tatggatgtt aaaataataa cagtaagagg
     2221 atgatttaag ctatggaaat attatcattg ggagaaaaaa taaaaagaag aagaaaagaa
     2281 ttaaatatga ctcttaagga attagcgggg gagagaataa caccaggaca aatcagttta
     2341 gtagagtcag ggagatcaaa tccttcaatg gatcttttag agtatattgc aatcacgcta
     2401 aatacttccg ttgaatattt aatggaaacg gaaaaaacac aagctgaaaa aatatgttta
     2461 tattacgagc aaatagcaga aagtgctatt ttaaatgatg atataaaaaa gggtgagaaa
     2521 tttatagaat atgcaatata ttatgctgat aagtatgacc taatatatag aaaagctaaa
     2581 aatttagcat taaaggctga aatgtattta aaaagtgaag aatacctttt agctcagcaa
     2641 tatttcttat ctgcaaatat attattttta caaataaata gcaatgtaga tatggtaaaa
     2701 acatacttaa atatagccaa aataagcttg cattcaaaag catattattc agctaattca
     2761 tatttaaaag aagctgaaaa gatatacata aataataaat taactgaaga tttacttcta
     2821 ggagaaatat attatttaat atcaaaaaca tacttcttta ttgaaaatat acaaaaggcg
     2881 ataaattttg catttttagc aaaaagcgaa tttgaaaaag tttacaataa aaaagcttat
     2941 gctaagagtt tagaattact agcagaagag catgctaaaa ctggtgattt agaaagtgca
     3001 atagcatact caaagcaaag tcttaatgtt tataaagaat tagagcaatt agataaaata
     3061 agttcaatag aagattcctt aggaaaatta ttctttgaat ttgacaatat agaagagtcc
     3121 tttgaacatt ataatagagc tataaaaata agagaagaaa acactgattc taaactttta
     3181 gatactttat ttaatttatg tgaaaatcat attaagacaa aaaatatgaa taaatgcata
     3241 gaaattctta atgaaataaa aaataaatta aatgtaagtg atgttaatag attaatagaa
     3301 tataagattc tgacttttag aatagaagta ttacaaaaca atataaaaga agcagaaaat
     3361 attttattag atacacttga aattgctaag agaaataata tgaaaaagaa agaagctgat
     3421 atctccataa gactaggtaa attctattta gagcttaaga gagattatga agcggctaaa
     3481 tacttagaca agggtataaa taatctgaaa aatttaggtg taattaaaag ttaaggtata
     3541 ggtgagagtg atggattttt tatctactgg ggagaaaata aaaagagcta gaatatataa
     3601 aggtttaaca ttaaagcaac tttgtgaaga tgatatatcc atatctagaa tgagctgtat
     3661 agagaatgga aaagtaaaag cagataaatg ggtgctagag ttagttgcta aaagattaga
     3721 tttagattta gagtatcttt tatgtgatga tataacacaa ctaaataatt ctattgataa
     3781 gtatattcaa aagaaagtct taactgaaag tgaagctgat gagataaaag agtacttaga
     3841 atattcttta agcaaagaat atgaagatat ttctgcaaaa cttatgcata ttttatttat
     3901 aaattatact aatattagag aatttaaaaa agcaaaagat ttgttaaatg agtattcaaa
     3961 catatacgaa agcaataaag agagtaaaag attatattat gaggatttag ctttatattt
     4021 tacagtaaga aagaattttg cagatgcaat aacatattat aacatgcttt tagatttcct
     4081 attaaaagat ggaatagaaa aaaataatga gatttatata aaaaatgcta cagctttagc
     4141 aatatgtaat tacagagttg gtcatataga gaaaagtaga gaaataataa aagatatttt
     4201 ctctatacaa ggaattgatc ttaatagtaa gtgcttaggg gaagcccaag gaattttagc
     4261 tattttatca cttctagaag gaaatagtga agagtttgaa aaaaattata cttattataa
     4321 agaaaagata gattgtaatt cttacaactg gaaaagaata aatagtctta ttattgatgc
     4381 tttcttaaaa tgcggaaaat atgaagaagc ccaaaagctt actgaagaat ctatagaaca
     4441 ggttaataag gaagataaaa tagggtatat agaactttta ttatttataa taaatgttta
     4501 caatgctaat ggatgtatag aaagtgttaa ggaatattgt gaattatcac tagaattagc
     4561 aataaataaa aataatatgt tctttataga gaaaggttat tattttaaag cagaattaag
     4621 taaaaatgag agaaatgata tgcaatggga aatgtatatg aatttagcaa cagatctttt
     4681 acttaaattt ggtacatatg aagagaaaag agaaagatat ttagaaatgg ctgatcttta
     4741 tcactattta gatgatacta atgaagcttt aaaatattta agttttgcta taaaagaagt
     4801 ggataattta tattaatata gaattaagga gttacatcaa aattagtaat acac
//
LOCUS       DAEVGI010000160         4852 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEVGI010000160 DAEVGI010000000
VERSION     DAEVGI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.161"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     regulatory      862..944
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            1007..3181
                     /locus_tag="K8M77_002209"
     CDS             1007..3181
                     /locus_tag="K8M77_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBI7086672.1"
                     /translation="MKNSKNFNIFTLWSVVISMILIMISPGSIVLGEETKSVQNDGAV
                     EITSTTFESNTVAKGISNNLRIDYKILNKDKLKDGDKIVISLPDIFKDIEPKCHDQHF
                     KDFDVKDGVVTLTFNENVEKAVTGYMIIRFVGNSNIRKGVSYPVSIDLNGKPSTVYIT
                     GEEYSNSSSGGQYPLMYKTADLPTAATDKEQGREYYGEIVDRNKPIKYFVEINLGDGV
                     NPNTRSYLSNADFFDNIPKGMALDINSICIKRMGYYDERSSDVTKDFWESNRIKADTK
                     HLEINFGDIRYERYTIIYETRITSTESGYLNDAKLYYDDDKELPSKHYSKLSKDAGAL
                     NVYKYVDKTKVTNNPNDQKIKYDIKFDSYGYFFKDTLNIIDKLDPRLSDIKITATDQF
                     ITDFYENTKELVIKNSNGDIDAKKPAYITIEASMKNVGPGEVVKNIAYVNGNPTNEVS
                     TRKNPIVEIIKVSKDDVESNLLEGAVFKLTTKDGKTVKDVYNQLVKTFTTNSEGPIRF
                     ELPNGDYKLEEIKAPNGYKLDQTPIEFTVNDESKIVKVVAKDEPLPTTCNLVINKINE
                     KEIPILGAKFKLFEESNPKKVLKFSSQKNNYELNQNGVGKLTPSGKNASFKINNLPYG
                     NYILKEVQAPKGYKLSDDIYITLGFEESFYRVGKQGEKIILNKNTETNTYDISAENVP
                     RIILPETGGSGTSKFSFIGITLLAGVLSIVSTTKFVLKEREN"
     gene            3185..4753
                     /locus_tag="K8M77_002210"
     CDS             3185..4753
                     /locus_tag="K8M77_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBI7086673.1"
                     /translation="MKNNILKKFSFMMVFIFVFITTSIPVFAATPSISKDAPIKGSIT
                     ISKKGATFTAYKLLDATKSGDAYEYSVNSDLKDFFNNSNYGSYSQESIQKLSGEEVKE
                     FAVNLHKYILDNKKSGQELTDGQKNTVDLGYYLVTETSSDSEGAAVASTPIIVSVPQV
                     SGDSWNYDVTINPKDNTPILEKNIVKDNQRVKTSSENIGDVIKYEVKASIPVYQKNAQ
                     NIMYKFTDTMSKGLTYDEKTGFKVTSGDKVFAKDTDYTVDVKKQEDGETVITINFVYE
                     NIKAYAETGITLNYQATLDKDAVISNKENLENTNNIELDYTNNPHLKDSYKKLTDKVT
                     TYTFGFGITKVDSEINSKLLQGAKFSVKDESGKTVAKYTYDEKGQVVSLSGNGVTNSK
                     GITTFLGLKEGKYLITEEVAPSGYSLLKNPVEVTITANKDESGNYTGAATIEISNGNK
                     AGQIINDISENDGNILFNIQIENHAGFSLPSTGGLGNTGFIKIAIILLSIVCVLSILG
                     LGYTKFENSRKTKN"
BASE COUNT         1994 a          502 c          836 g         1520 t
ORIGIN      
        1 gaagaaactt aagcttgtta ttagaagttt taaatttagt tgatagaata acttcaaaag
       61 aaaatgaaga taaattaaga tatttaaatg atatattaaa tgggtaaaat atttaaattt
      121 tgcttatatt ttataatata aaaaataagg ttaaagagct attattttaa tattttatcc
      181 tttagtgacg gagttgtgac gtatttttga cggagttgaa acctgagttt ttctagtgat
      241 atcaatggtt ttgacggaag tgacggactt tttccttttc tggtatatag ggggaaaata
      301 aaaaatataa aaaaatatat atataaataa aaaaagtata cagtatacag ggaaatagaa
      361 aaaagtccgg caaagtccgt cataggtagt tctaagtaag ttatatcaag ggtttaaagc
      421 gtgtgacgga cttataaaag tctgtcaaaa gtccgtcaca atacttttag agattaaatt
      481 tacatggtct aagtgggaaa aaaattctat aaaaataatg tgtggctttg taattatgtt
      541 ccaaaaaata cattaaagaa ttaaatttag agtggaaaat ttttaagtgc attttaaatt
      601 aaaaatataa aattaatatt aaaaacctca taaagctttg tgttaaaagt ataaaaaaat
      661 cgaaaattat ttgataaatt aattgcaaaa taatataaaa tatataagtt taacaataaa
      721 aacttaataa aattaacata taatatttgc tgagaaaaaa tgttttttga caggtttttt
      781 aaaatactgt aacatacaaa tacaaattga aaattaagtg ttaaaaagta gtgattttta
      841 agaaataatt agtaaatatt aatatgttat agaaacgttg atgtatattc tgtatttggg
      901 catctgggat atactgagtt agtgatgcaa ccgaatatat ttataaaaag ttataaatat
      961 agtcggtttt ttattttcca ttttttaaga gaggagggct tattttatga agaatagtaa
     1021 aaatttcaat atatttacct tatggtcagt tgttatttct atgatattaa ttatgatttc
     1081 tcctggaagc attgttttag gagaagaaac aaaatcagtt caaaatgatg gggctgttga
     1141 aattacaagc actacttttg agagtaatac tgttgctaaa ggcataagca ataatttaag
     1201 aattgactat aaaattttga ataaagataa gcttaaagat ggtgataaaa ttgttataag
     1261 tttgccagat atatttaaag acatagagcc taaatgtcat gatcagcatt ttaaggattt
     1321 tgatgttaag gatggagtgg tgactttaac ctttaatgaa aatgtagaaa aagctgtaac
     1381 aggatatatg attataagat ttgtgggaaa tagcaatatt agaaagggag taagttaccc
     1441 agttagtatt gatttaaatg gaaagccaag cactgtttat ataacaggag aagaatattc
     1501 caattctagt tctggtggac aataccctct tatgtataag actgctgatt taccaacagc
     1561 tgctacagat aaggaacaag gaagagagta ttatggagag atagtagata ggaataaacc
     1621 tataaaatat tttgtggaaa taaatttagg tgatggagta aatccaaata caagaagtta
     1681 tttaagcaat gctgattttt ttgataatat tccaaagggc atggctttag atataaatag
     1741 tatatgcata aaaagaatgg gttactatga tgaaagaagt agtgatgtta ccaaggattt
     1801 ttgggagagt aatagaataa aggctgatac caaacattta gaaataaatt ttggtgacat
     1861 aagatatgag agatatacaa ttatctatga aactcgtatt acttctactg aaagtggata
     1921 tttaaatgat gctaaattat attatgatga tgataaagag ttgccttcaa agcattattc
     1981 aaagttatca aaggatgcag gagcattaaa tgtttataaa tatgttgata agacaaaggt
     2041 aacaaataat cctaatgacc aaaaaattaa atatgatatt aagtttgatt cctatggata
     2101 cttttttaag gatactttaa atattattga taaattagat cctagactaa gtgatattaa
     2161 gattacggct acagatcaat ttataactga tttttatgaa aacacaaagg aattagttat
     2221 taaaaatagt aatggagata tagatgctaa aaagccagca tatatcacca tagaagcaag
     2281 tatgaaaaat gtagggccag gggaagtagt aaaaaatatt gcatatgtaa atggaaatcc
     2341 aactaatgag gtttcaacta gaaagaatcc tatagtggaa ataatcaagg tttctaaaga
     2401 cgatgtagaa tcaaatttac ttgaaggagc agtttttaaa ctaacaacta aagatggtaa
     2461 aactgttaag gatgtgtaca accaattggt taaaacgttt acaacaaatt ctgaagggcc
     2521 aataaggttt gaattaccta atggagatta taaattagag gaaataaagg ctcctaatgg
     2581 atataaactt gatcaaacgc ctattgaatt tactgtaaat gatgaaagta agatagttaa
     2641 ggttgtggct aaggatgagc ctttaccaac tacttgtaac ttagttataa acaaaataaa
     2701 tgaaaaagaa attcctattt taggagctaa atttaaatta tttgaagaaa gtaatccaaa
     2761 aaaagtttta aaatttagtt ctcagaaaaa taattacgaa ttaaatcaaa atggagttgg
     2821 gaaattaaca ccttcaggga aaaatgcatc ttttaaaatt aataatttac cttatggcaa
     2881 ttacatatta aaagaagtac aggctcctaa gggatataaa ttaagtgatg atatttatat
     2941 aactctaggt tttgaagaaa gtttttatag agttggaaaa caaggtgaaa aaataatttt
     3001 aaataaaaat acagaaacaa atacatatga tatttcagct gaaaatgttc caagaataat
     3061 actaccagaa acagggggca gtggaacttc taaattttca ttcattggaa ttacactttt
     3121 agcaggtgta ttaagcatag ttagtactac aaaatttgtt ttaaaagaaa gggagaatta
     3181 atagatgaaa aataatatat taaaaaagtt tagctttatg atggtgttta tttttgtttt
     3241 tattacaaca tcaatacctg tatttgcggc tacgccatca atttctaaag atgctcctat
     3301 aaagggaagt atcacaatat caaaaaaagg tgctacattt actgcatata agttattaga
     3361 tgcaactaaa agtggggatg catatgaata ctcagtaaat agtgatttaa aagacttctt
     3421 taacaattct aattatggtt cttatagcca agaatcaatt caaaagttaa gtggagaaga
     3481 agtaaaagag tttgctgtta acttacataa atatattctt gataataaaa agagtggaca
     3541 agaacttaca gatggacaaa agaatactgt tgacttaggt tattacctag ttacagaaac
     3601 ttcaagtgat tcagaggggg cagcagttgc ttcaacacct ataatagttt cagttcctca
     3661 agtttcagga gattcatgga attatgatgt aactattaat ccaaaggata acactcctat
     3721 attagaaaaa aacatagtta aagataatca aagagttaaa acttcatctg agaatattgg
     3781 agatgttatt aaatacgaag taaaagcttc tataccagtt tatcaaaaga atgcacagaa
     3841 tataatgtac aaatttactg atactatgag caagggatta acatatgatg agaaaactgg
     3901 ctttaaggtg acttcaggag ataaagtttt tgctaaggat acagattata ctgtagatgt
     3961 aaaaaaacaa gaagatggag aaacagttat tacaataaac tttgtatatg agaatataaa
     4021 agcttatgca gagactggaa taactttaaa ttatcaagct accttagata aggatgcagt
     4081 tattagtaat aaagaaaact tagaaaacac taacaatata gaattagatt atacaaataa
     4141 tccacatctt aaggatagtt ataagaaatt aactgataag gttactactt atacctttgg
     4201 atttggaatt acaaaggttg attcagaaat taattctaag cttttacaag gtgctaaatt
     4261 ttcagtaaaa gatgaaagtg gaaagactgt agctaaatat acatatgatg aaaaaggaca
     4321 agtagtttct ttaagtggaa atggggtaac taactcaaag ggaattacaa cattcctagg
     4381 cttaaaagaa ggaaagtacc ttattacaga ggaagtagct ccatctggat atagcttatt
     4441 aaaaaatcca gtagaagtga caattacagc taataaagat gagtctggaa attacacagg
     4501 agctgcaact atagagattt ctaatggaaa taaagcagga caaataataa atgatatttc
     4561 agagaatgat ggaaatatat tatttaatat tcaaatagaa aaccatgctg gattttctct
     4621 tccatcaaca ggaggattag gaaatacagg atttataaaa atagctatta tcttattaag
     4681 tatagtttgt gttttatcta tactaggact tggctacact aaatttgaaa atagcagaaa
     4741 aactaagaac taaacttaaa aattaaatac agagctataa accaaaatac aataacaaaa
     4801 cttacaaaca gtatgaagag aatatctaga tgagctttag aatttaaatt at
//
LOCUS       DAEVGI010000161         4852 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEVGI010000161 DAEVGI010000000
VERSION     DAEVGI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.162"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            67..1149
                     /locus_tag="K8M77_002211"
     CDS             67..1149
                     /locus_tag="K8M77_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI7086674.1"
                     /translation="MDIISSINTRFLSKSEQSKKEIFNKIDNIVKGGGNIIRMNLSHG
                     KHRDVECCIDYIRSNHKNVKILLDLQGNKIRVANNIYGTFKVNSGDLVYFCSEETYDV
                     YLKNVDRNKLIPLNIKNKFIYNNTFKKIYMKDATMEFIVISNNNGLIKTKVKLGGVVR
                     KEKGCNLPNLDRKNWGVSEKDLEDIKFAIDNKVDIIDYSYCSYMEECREFKNIVFKNL
                     KSNQFIPKLWGKIETNEGINNIKEVAKELDGIVIARGDLTAEIGILNVPIVQEKILYA
                     LKNENKSIIVATNVLSSIRNKQNKPTINELSDIYHFIRCGATGFMLTGETSTGENEEY
                     VVTTLKNSIKYYEKLLSKIKKRSKGV"
     gene            complement(1146..1703)
                     /locus_tag="K8M77_002212"
     CDS             complement(1146..1703)
                     /locus_tag="K8M77_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBI7086675.1"
                     /translation="MHFIQNIDINILYFIQNNIQNKFLNPIMIFLTSLGNLGFIWIAL
                     SILLIISKKYRKIGLLTLAVLIVNTLLGEGILKHIIERSRPFATYESLHIMIPKPSSY
                     SMPSGHTSASFASAFMLAYYFKNIRIYIYSLASLIAFSRLYLLVHYPSDVLTGILLGY
                     LSYLIVIKSYELIQKSKIKDQRKSF"
     gene            2506..3954
                     /locus_tag="K8M77_002213"
     CDS             2506..3954
                     /locus_tag="K8M77_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI7086676.1"
                     /translation="MLRHKKLLALILGGVMTTSVMLTGCGSSDKKAEGGAASEEPVNL
                     VWYVIGKPQTDGELVEEEVNKYIKDKINATVDIKHIDFGDYSQKMNVIANSGEEYDLA
                     FTCSWAFPYLENARKGAFLELNDLLDKEGADLKGVIDERLWKGAEVDGKIYGVPNQKE
                     IAGAPMWVFDKELVEKYDIPYQDLHSVEDLEPWLQIIKEKEPDFVPFYTQGDSIPLEF
                     DEIMRPLGVFFNDDTLTVQNMYETEEMKAMMTKLREYYEKGYINQDAAVNNMKNEVKR
                     FMWKADGQPYAENGWGQALGREVVTSSIIPPYVTNNSTTGAMTAISATSKHPEKAMEL
                     INLVNTDSTLRNLLMFGIEGTHYEKVSDNQIKRDPNGPYSVTSWAYGNLFDTYVLDSD
                     PADKWDAFEEFNQGAKTSPILGFKFNTEPVTTQISAINNVLQEFERTLYSGSVDPVKG
                     LDDLNKKLAASGLDDIKAEMQKQLDEWKASNK"
     gene            4104..4838
                     /locus_tag="K8M77_002214"
     CDS             4104..4838
                     /locus_tag="K8M77_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HBI7086677.1"
                     /translation="MEDKIKIFKALGDETRINILHLINKKSMCAKGIARELSISESAV
                     SQQIKVLKDSNLVIAYKIGYQIVYDLNIEQLESINILIDYIVNDYNNIKTKENIYEKC
                     NKICKHAKCINNKNLKEENIMRICFPVKNNEGVKSVPYGHFGTAPTFVICDLEKDEVT
                     TVGNGDLGHEHGKCQPIKALSGEVVDAVVVGGIGAGAISKLNSMGIKVYKAIDGDIEV
                     NLESLKKGELKEFSSTHTCNHHGCSH"
BASE COUNT         2035 a          465 c          825 g         1527 t
ORIGIN      
        1 tataaataat atttaaaagt cattatgcat ataaatcaat tagtagcaat atttgtgggg
       61 gaagttatgg atattataag cagtataaac accagatttt taagtaaaag tgaacagagt
      121 aaaaaggaaa tttttaataa aatagataac atagtaaaag gtgggggaaa tataattagg
      181 atgaatctat cccatggcaa gcatagagat gtagaatgct gtatagatta cataagaagc
      241 aatcataaga atgtaaaaat tcttttagac ttacagggaa ataagattag ggtagctaat
      301 aatatatatg gaacatttaa agttaattct ggtgatttag tttatttttg ttcagaggaa
      361 acttatgatg tttatcttaa aaatgttgat agaaataaac ttataccttt aaatataaaa
      421 aataagttta tatacaataa tacttttaaa aagatatata tgaaagatgc cactatggaa
      481 tttatagtaa taagcaataa caatggatta ataaagacta aagttaaatt aggtggagta
      541 gttagaaaag aaaagggatg taatcttcct aatttagata ggaaaaattg gggtgtaagc
      601 gaaaaggatt tagaagatat taaatttgcc atagataata aagtagatat tatagattat
      661 tcttattgtt cttatatgga agaatgtaga gaatttaaaa atattgtttt taaaaattta
      721 aagtcaaatc aatttattcc taagctttgg ggaaaaattg agactaatga aggaataaat
      781 aatattaaag aagttgctaa ggaattagat ggaatagtta tagcaagggg agatttaaca
      841 gcagaaattg gaatattaaa tgttcctata gtgcaagaaa aaatattata tgctttaaaa
      901 aatgagaata aatcaataat agtagccaca aatgtacttt catctattag aaataaacaa
      961 aataagccta caataaatga attaagcgat atatatcatt ttattaggtg tggcgcaaca
     1021 gggtttatgc ttactggaga aactagtact ggggaaaatg aagagtatgt agttacaacc
     1081 ttaaaaaata gtataaaata ttacgaaaag ttattatcaa aaataaaaaa gagatcaaag
     1141 ggagtctaaa aagactttct ttgatctttt attttagatt tttgtataag ttcataggat
     1201 tttataacta ttaagtagct tagatatcct agtaaaatac ctgttaatac atcacttgga
     1261 tagtgaacaa gcaagtataa tctagaaaaa gctattaaag aagctaagga atagatatat
     1321 attcttatat ttttaaaata ataagctaac ataaatgctg atgcaaagga tgcagaagta
     1381 tgaccagagg gcatagagta actacttggt ttaggtatca ttatatgtaa gctttcatat
     1441 gttgcaaagg gtctagatct ttctataata tgtttaagaa ttccttcacc aagcaaggtg
     1501 ttaacaatta aaacagctaa ggttaataat cctatttttc tatatttttt tgatattata
     1561 agtaagatac ttaaggcaat ccatataaag cctaaattac ctaatgaagt taagaaaatc
     1621 attattggat taagaaactt attttgtata ttattttgta taaagtaaag tatatttata
     1681 tctatatttt gaataaaatg catcttattg cctcctaaaa ttaataaata tattttatta
     1741 ctaaattttt aagaagtcta ctataagtat gtaaataagt ttataaaaaa aagtaatatt
     1801 atataaaaaa tattcattta aagaattaga gaaacaaaat ataatgtaga tgtaataaat
     1861 aaaatgaggt taatagtaag tctaatgtat ataaagtctt aggatttaag tttgacacta
     1921 gtaatgtaac tgttgaatta gagtctgtaa atactgttct tgaagagttt acaagaattt
     1981 tatatactgg actattagat acagatgaat atgttttaaa gcttattaat aagctttaaa
     2041 gtgctggaat agatatggtt aaggaagaat tagaaaggca attagataat tggaaaataa
     2101 aaataaattt taatcatata tgattagata aagaataaaa atactcaaaa tgtaatagtt
     2161 atattacaaa aaattcaaac tttttaaaat atttaaaaag ttaacagaat atataaaaaa
     2221 atcttttgat gttaaaaatc tttgaaatgt agtaaaatag cctatttaac acattaatat
     2281 agtacttttt tgtaatgttg actttttaac tgttatgtta taaattaact atagaaactt
     2341 taatttactt aaaacttctc aaacaaaata ctaaaaaata tatatgatta tcatattggt
     2401 tttaattaaa tttgataaag tgtatgattt ttgtaaacgt ttgtttagtc ttttgaatta
     2461 agggatctaa atggtataaa aatagtaata tgagggggaa aaaaaatgct tagacacaaa
     2521 aaattattag cattaatttt aggaggagta atgactacat ctgtaatgct tacaggatgt
     2581 ggatcaagtg acaaaaaagc agagggagga gcagcaagtg aggaaccagt taacttagta
     2641 tggtatgtta taggtaaacc tcagactgat ggagaattag tagaagaaga agtaaataaa
     2701 tatataaaag ataaaataaa tgctactgta gacataaaac atattgactt tggtgattac
     2761 agccaaaaaa tgaatgtaat agctaactca ggagaagaat atgatttagc atttacatgt
     2821 tcatgggctt tcccatactt agaaaatgct agaaaaggtg ctttccttga attaaatgat
     2881 ttattagata aagagggagc agaccttaaa ggggttatag atgaaagact ttggaaaggt
     2941 gctgaggttg atggaaaaat atatggagtt ccaaaccaaa aagaaatagc aggagcacct
     3001 atgtgggtat ttgataagga gcttgttgaa aaatatgata ttccatatca agatttacac
     3061 tcagtagaag atttagaacc atggttacaa atcataaaag aaaaggaacc agattttgta
     3121 ccattctata ctcaagggga ttcaattcca ttagaatttg atgaaataat gagaccttta
     3181 ggagtattct ttaatgatga tactttaaca gtacaaaata tgtatgagac agaagaaatg
     3241 aaggctatga tgactaaatt aagagaatac tatgaaaaag gatatataaa tcaagatgca
     3301 gcagttaata atatgaaaaa tgaagttaag agatttatgt ggaaagctga tggacaacca
     3361 tatgcagaaa atggatgggg acaagcttta ggtagagaag ttgtaacatc atcaataatc
     3421 cctccatatg ttacaaataa ttcaacaact ggagctatga ctgctatatc agcaacatct
     3481 aagcatcctg aaaaagctat ggagcttata aacttagtaa atactgactc tacattaaga
     3541 aacctattaa tgtttggaat agagggaact cactatgaaa aggttagtga caatcaaata
     3601 aagagagatc caaatggacc atatagtgtt acaagttggg cttacggaaa cttatttgat
     3661 acttacgttt tagatagtga tccagcagat aaatgggatg cttttgaaga atttaaccaa
     3721 ggtgctaaga cttcaccaat cttaggattt aagttcaata cagagccagt tacaactcaa
     3781 atatcagcaa ttaataacgt attacaagag tttgaaagaa ctttatactc aggttcagta
     3841 gatccagtaa aaggattaga tgacttaaat aaaaagttag ctgcatctgg attagatgac
     3901 ataaaagctg aaatgcaaaa acaattagat gaatggaaag cttctaataa ataataaatt
     3961 ttaaatataa aaagagtggt tttaagccac tcttttttag tttttaaata tttaatttaa
     4021 agtaattcta atatagaaaa gagtaaaata ttaaaaaata aatttaatta agaaaatact
     4081 taattaaaaa taagaggtgg cttatggaag ataaaataaa aatatttaaa gcattaggag
     4141 atgaaactag aataaatata ctacatctca taaataaaaa atctatgtgt gcaaagggaa
     4201 tagctagaga acttagcatt tcagaatctg ctgtttctca acaaataaaa gtattaaaag
     4261 attcaaatct cgtaattgca tataaaatag gttatcaaat tgtttatgat ttaaatatag
     4321 agcagttaga aagtattaat atcttaattg attatatcgt aaatgattat aataatataa
     4381 aaacaaaaga aaatatctat gaaaaatgca ataagatatg taaacatgct aaatgcataa
     4441 ataataaaaa tttgaaggag gaaaatataa tgagaatctg tttcccagtt aaaaataatg
     4501 aaggagttaa aagtgtacca tatggtcatt ttggtactgc tccaactttc gtaatatgtg
     4561 atttagaaaa agatgaagta actactgtag gcaatggtga tttaggtcat gaacatggaa
     4621 aatgtcaacc aataaaagct ttatcaggtg aagttgtaga tgctgtagtt gttggtggaa
     4681 ttggtgctgg tgctataagt aaattaaatt ctatgggaat taaggtttat aaagctattg
     4741 acggagatat agaagttaat ttagaatcat taaaaaaagg tgaattaaaa gagttttcaa
     4801 gcacacatac atgcaatcat catggatgca gtcattaata aagaaaaagc ga
//
LOCUS       DAEVGI010000162         4745 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEVGI010000162 DAEVGI010000000
VERSION     DAEVGI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.163"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            417..1256
                     /locus_tag="K8M77_002215"
     CDS             417..1256
                     /locus_tag="K8M77_002215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086678.1"
                     /translation="MNFIDTYDEILSYFSSKEFNISLWENYSNVISKELSNKVKRDIK
                     DYNYRKDILPVVETALKSREKLKQVHESFIAVSDNIKYKFNELFEEDLDLDIILYLGL
                     CNGAGWATSLDNKNVVLLGIEKIIELDWCNEEDMFALIAHEIGHIWHKTKGGCFGKQK
                     NISEKALFQLYSEGVAMLFEQLLCGDENYYHQNQEGWIDCCENNLIELKREYLIRITN
                     SESVQDFFGDWNSYKGHSDVGYFIGCKFVRFLLKKYSLTEMLSMEMDLMLKEYISFSK
                     LGR"
     gene            1444..1875
                     /locus_tag="K8M77_002216"
     CDS             1444..1875
                     /locus_tag="K8M77_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HBI7086679.1"
                     /translation="MTESDFLEKLKELKNIICVVEFGSFRTESWIKDRSDIDLLVITA
                     PNVTFMDTLEIEDDVLEIAREFYKYPNIHLTFLLFKDFHNKFAKIAIDSEKKYILNEE
                     KWYDFNHNVLKYIRINERLNRNLKLDEQFSYFGGIIDESIL"
     gene            1859..2317
                     /locus_tag="K8M77_002217"
     CDS             1859..2317
                     /locus_tag="K8M77_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086680.1"
                     /translation="MSPYYRYKKEKFLEKYQSATDNLEDLKVAIKEYKDTNNRIIRRA
                     IFAFFQDFTEYIIDMCESYILINDGKIKSTTSATQLVEQAYDMGFFDNELKKYLVLSV
                     KLRNRYTHDYYLREESEKAIEEFCFLKLANLEIFLEESKDKVILKYKNLV"
     gene            complement(2391..2786)
                     /locus_tag="K8M77_002218"
     CDS             complement(2391..2786)
                     /locus_tag="K8M77_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HBI7086681.1"
                     /translation="MKKTVKVVGAIIENEKKEILCALRSKDMTLPKQWEFPGGKIEEG
                     ETVGQAIVREIKEELDCDIEYISTFNDNTHEYEKVIVNLITLRCKLVNGTPKASEHAK
                     LIWLPVDYLETLSWAPADVPAVKQLVSEK"
     gene            3160..3369
                     /locus_tag="K8M77_002219"
     CDS             3160..3369
                     /locus_tag="K8M77_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086682.1"
                     /translation="MNKIKEYRSLANMSQLELSKKVGVARQTINLIENQKYNPSLDLC
                     IRIAKALGTDLNTLFWVNEDEREDE"
     gene            3362..3868
                     /locus_tag="K8M77_002220"
     CDS             3362..3868
                     /locus_tag="K8M77_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086683.1"
                     /translation="MNKFEKTLLKHNIGYLDERDEYQQGVIYETLATANLYLSYLLII
                     CMVISLIFDGVNHSLTFGTFALAALQFGNSCYILNKLKKSGAYETEVYDDYTYNMEIK
                     KLKKQCIISAIQWGIFMFFVMTYLWPWILGEPINVGLRQVIAWTIGGTLFGLLCYAIS
                     KSKIKKIV"
     gene            4303..4479
                     /locus_tag="K8M77_002221"
     CDS             4303..4479
                     /locus_tag="K8M77_002221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086684.1"
                     /translation="MSEENKELIIEEVEIKGKKRKINLEKYAKFFSDKDFFEKLRKFA
                     KKAGIKVVYMSLLL"
BASE COUNT         1862 a          487 c          746 g         1650 t
ORIGIN      
        1 aaaagtcata tataattaat ttattattta gacattataa caatggaaat actatatgat
       61 atatatatac aacctaaaat tgaggatgtt ttaaagtttc ttcaaaaaca atagacatta
      121 gagaaagttt aaataaatgt atagaaagtt agatgtaaaa taaaggattt atatataatt
      181 aagtttttat gtgtaacaga aaaatatttt gatatcaata gtatttttaa ggggcaaaat
      241 tataaattct cataaaaata acataatgaa tcaaaaaagt tctagatcat ttatttgctt
      301 tagaactttt tattatctgt attttaatgt tatggtaatt tgtggaataa aatttggact
      361 tgtgatatta taacacttat agataagcag aaaattttat ggtaggggtt ggtttaatga
      421 attttataga tacatatgat gaaattttat cttatttttc atcaaaggaa tttaatatat
      481 cactttggga aaattattca aatgtgattt caaaggaatt atctaataaa gttaaaaggg
      541 atattaaaga ttataattac agaaaggata tattgcctgt agttgaaaca gctctaaaaa
      601 gtagggaaaa attaaagcaa gtccatgaat catttatagc agtttcagat aatataaaat
      661 ataaattcaa tgaattattt gaagaagatt tggatttaga cattattcta tacttaggtc
      721 tatgtaatgg agctggctgg gcaacatcac ttgataataa gaatgttgtt ctacttggaa
      781 ttgaaaagat tattgaactt gattggtgta acgaagagga tatgtttgct ttgatagctc
      841 atgaaatagg tcatatttgg cataaaacta agggtggatg ttttggaaaa caaaaaaata
      901 ttagtgaaaa agcacttttt caactttata gcgaaggagt agctatgtta tttgagcaat
      961 tactatgtgg agatgaaaat tattatcatc aaaatcaaga gggatggata gattgttgtg
     1021 aaaataattt aattgaacta aagagagaat acttaataag aattactaat tctgaaagtg
     1081 tacaagactt ctttggagat tggaatagtt acaaaggtca ttcagatgta ggctatttta
     1141 taggatgtaa atttgttaga tttcttctaa aaaaatattc acttacagaa atgctttcta
     1201 tggaaatgga tttaatgtta aaagaatata ttagcttttc aaagttagga aggtaattaa
     1261 tatggagagg atagttgtag ttaaagatga attatttatt aaggaagagc tattgaataa
     1321 ttattaaagt tataaatgaa aacagggtat ttctcctgtt ttttatttct catattttgt
     1381 ataagtttta aaagtgaaag tattaggata taataagatt aggaataaaa tgaggtgatt
     1441 aatatgacag aatcggactt cttagaaaaa ctaaaagagt taaaaaatat aatttgtgta
     1501 gttgaatttg gaagttttag aacagaatca tggattaaag atagaagcga tattgattta
     1561 ttagtaatta cagcaccaaa tgtaactttt atggacacct tagaaattga ggatgatgtt
     1621 ttagaaattg caagagaatt ttataaatat cctaatatac atttaacttt ccttttattt
     1681 aaggattttc ataataagtt tgcaaaaata gcaatagaca gtgaaaagaa atacatatta
     1741 aatgaagaaa aatggtacga tttcaatcat aatgttttaa aatatataag aattaatgaa
     1801 agactaaata gaaatttaaa attagacgag cagtttagtt attttggagg aataatagat
     1861 gagtccatat tatagatata aaaaagaaaa atttttagaa aagtatcaat cagcaactga
     1921 caacttagaa gatttaaaag tagcaataaa agaatataaa gatactaata atagaattat
     1981 aagaagagct atttttgctt tctttcaaga tttcactgaa tatattattg atatgtgtga
     2041 aagttacata ttaataaatg atgggaaaat aaagagtaca acttctgcaa cacagctagt
     2101 tgaacaagca tatgatatgg gcttttttga taatgaacta aaaaaatact tagttttatc
     2161 agtgaaactt agaaatagat atactcatga ttattaccta agagaagaga gcgaaaaagc
     2221 aatagaagaa ttctgttttt taaaactagc aaatctagaa atatttttag aagaaagtaa
     2281 ggataaagtt attcttaaat ataaaaattt agtttaatag ataacttcac aggggtttta
     2341 tgatagtata attcaagaag taagaaaaac aggagaaatc tcctgttttt ttatttctca
     2401 cttactaatt gctttacagc cggaacatca gcaggagccc atgaaagagt ttcaaggtaa
     2461 tctactggaa gccatataag ttttgcgtgt tcactagcct ttggagttcc attaacaagt
     2521 ttacatctta gtgttattaa gttaactata actttctcat attcatgagt attatcatta
     2581 aatgtagata tgtattctat atcacaatct aattcttctt taatttccct aactattgct
     2641 tgtcctacag tttccccttc ctcaatcttt ccacctggga attcccattg cttaggaaga
     2701 gtcatatcct ttgatcttaa ggcacaaaga atttctttct tttcattttc aattatagca
     2761 ccaactactt ttactgtttt tttcataatg tttttctcac ctcgaagatt attttatcat
     2821 aattacaatt gagatcaaac tttagattaa cttataaata attactggtt atatttagat
     2881 aagaaatgta atattagatt atctatgaca ccattatcac atgaaacaat tcctcacaat
     2941 aggtaggcct ccatttctca taattcatat aatcctcatt aaaggtaaaa cgcttgtcca
     3001 taatacacct cttaaaaatt aaatttatac cataatattt tttatacaat cttattatat
     3061 cattaaactt aaagtgtcac atatatttga catataaaat ttttaatggt attattttac
     3121 taaagtcata tatatgtgat acggaattga ggtgaaggga tgaacaaaat aaaggaatat
     3181 agatctctag ctaatatgtc acagttagag ctttcaaaaa aggttggagt tgcaagacaa
     3241 accataaatt taatcgaaaa tcaaaaatat aatccatccc tagatttatg tattaggatt
     3301 gccaaggcct taggaactga tttaaacaca ttattttggg ttaatgagga tgaacgagag
     3361 gatgaataag tttgaaaaaa cacttctaaa acacaatata ggttacttag atgaaagaga
     3421 tgaatatcag caaggagtga tttatgaaac tcttgcaacg gctaatttat acttatcata
     3481 cttacttata atttgtatgg taattagtct tatttttgat ggagttaatc atagtctaac
     3541 ctttggaaca tttgccttag cggcccttca atttgggaac tcatgttata ttttaaataa
     3601 gctgaaaaaa agtggagctt atgaaacaga ggtttatgat gattatactt acaatatgga
     3661 aattaaaaaa ttaaaaaaac aatgtataat atcagcaata caatggggaa tatttatgtt
     3721 ttttgtaatg acatatcttt ggccatggat tttaggggag ccaattaatg taggattaag
     3781 acaagtaatt gcttggacaa ttggtggaac tctatttgga ctattatgtt atgctatttc
     3841 aaaatcaaag ataaagaaaa tagtgtaaat ttagagactt gctaactaaa gtaagtctaa
     3901 taagatttat ttaacttggg aattatatta tataaaggaa taggttttaa agaaagttac
     3961 aggagtctag gcagaagaat tatattataa gaactagaaa tatattatat agaagaataa
     4021 agttaggata aataaacaag gattataatt ttagatctca atattttttt gtaaaatagc
     4081 tttactttaa gaagaaatag cattttaaat ttagcctcct atttgctagg attcttgcaa
     4141 aaatataaga aatagcagtt gatcaataaa taaattttaa tcttatatct actaagtttc
     4201 ttgaaaaaat ataagaaata gtatctaaga aaatattatc tattatatgt tgaaataaac
     4261 ataacattag gagtattata tattgcaaag gaggggtttt gtatgagtga agagaacaag
     4321 gaactaatca ttgaagaagt tgagataaag ggtaaaaagc gtaaaataaa tttagaaaaa
     4381 tacgcaaaat tcttttctga taaggacttc tttgagaaat taagaaaatt tgctaaaaaa
     4441 gcaggtatta aggttgttta tatgagctta ttattatgaa atattaggta attacattta
     4501 ttaagtaaaa gaaagaagaa ctaattcata ggttactctt tcttttttat ttcaataata
     4561 taaatatatt aagttatact gattttaaaa aattttaggc catatgaaag gttggatttg
     4621 tgtcaatatc ttggacataa aaattcgaac ttacttaagc tgcattcaaa gccttaaatt
     4681 ctacctcatt aggagtatta taatcaagag cttaatgtag ccttttacta ttgtaaaaaa
     4741 ctttc
//
LOCUS       DAEVGI010000163         4709 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEVGI010000163 DAEVGI010000000
VERSION     DAEVGI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.164"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            56..1552
                     /gene="yjeM"
                     /locus_tag="K8M77_002222"
     CDS             56..1552
                     /gene="yjeM"
                     /locus_tag="K8M77_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBI7086685.1"
                     /translation="MSSEHKSKLSLTALVLMIFTSVYGFANMPKAFYLMGYSAIPWYI
                     ISALAFSIPYAFMMAEYGAAFRKEKGGIYSWMAKSVGPKYAFIVTFMWYSSNLIWLVS
                     NSSSIWIPFSNVIYGKDTTGTWSLLGLSVPQTMAVLGSILIIVITYAASKGLKSITKV
                     TSIGGTVVALSNIVLILGAIIVFVSNGFKPAQIINGAAFVHAQNPSYQSPIGVLGFLV
                     FAVFAYGGLEAVSGLVDETKDAKKNFPKGLMIASIIIAVGYAVGILCVGLFTNWQEVL
                     SGDNVNLANVSYIVIQNLGVKLGQAFGMSTNASLQLGAWFARYIGLSMMLALMGAFFT
                     LSYAPIKQLIEGTPKEIWPKKWTILNENNMPVNAMWVQCIVVVIFILIASFGGESANK
                     FFSYLILMGNVAMTIPYMFLSGAFPAFKKKKHIEKPFIMYKSYKSSLIWSIIVTFTIG
                     FANLFTIIKPVIQDKDYTAMAFQITGPIIFSIIAFILYHNYEKKIKNK"
     gene            1667..2077
                     /locus_tag="K8M77_002223"
     CDS             1667..2077
                     /locus_tag="K8M77_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086686.1"
                     /translation="MKKKTLIILSIIFIVLLGIGFYFFSNQSTYKVIINNNTNKEISG
                     LQISYNNNKKNISIPSIKAGDSEEVNVKSNENGSLVMYYTDILGDSHKEILSGYFQKG
                     AQGKIVVNITSVNTLGIYGMEIASPEAENILNNK"
     gene            2127..2903
                     /locus_tag="K8M77_002224"
     CDS             2127..2903
                     /locus_tag="K8M77_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBI7086687.1"
                     /translation="MENIIYNILYIIIYSFLGWCCEVIYCYILDHKFTNRGFLNGPLC
                     PVYGFGAFFVLTFLDLYKRNLILVFILGIVITSILEYVTSFLLESIFHTKWWDYSKHK
                     FNIHGRVCLLNSTLFGILSVILVEFINPIIVDFVSKFPKKIIFLITIILLTLIAIDLI
                     VTVQAMIKLNANLHNLQNLSKEFEKLGISFIDKTEKELEITISKIKHESNLKEELLNK
                     VHSIRRNNLLEKRLLKAFPNMKHKRYNELLQKLRKYYKLK"
     gene            complement(2932..4194)
                     /locus_tag="K8M77_002225"
     CDS             complement(2932..4194)
                     /locus_tag="K8M77_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBI7086688.1"
                     /translation="MRSKRIKKLNSKKVALQISLVLIALFLILISVVLFKTYSSKNVT
                     YKGIYVENINVSNLNEKDLKEKLNEHYSDLLNNFKITFNYKDNFNETFSAKDLGFFFD
                     INDLSNQIFSFGKNSNIFSNTIERLKLKSNPKYFQFKPESNSKVLSEKLSEISNKINK
                     EKVEPTISFSNDSISATKEQIGYKLNLEKLTSEIVNDLNNSHDSLNCTINIPVEEIKP
                     KLTQETVNNMVILGSYTTKLPSLTGGRTKNISNYLSKLNGTVLMPGDIFSADKEGEDR
                     TESNGYTYAPGYIGNKVVDILAGGICQGVTTLYNSVLYADLKIVERAPHSLPVTYAPI
                     GRDATMASGSIDFKFQNNMNNPVIVQTYITSSGQVKSNIWGIKEDPGKEIVISVDQHG
                     PKSATTYKETYENGVLVKKEVLSNDKYN"
     gene            4451..>4709
                     /locus_tag="K8M77_002226"
     CDS             4451..>4709
                     /locus_tag="K8M77_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086689.1"
                     /translation="MFKKITLKDKSIYYKYIDKNKFLSCEYSFTTLFMWKDFNNIEYD
                     IVNNIFIIRKYDKINGKIFMQPLGDIDDDSLINIIDYLEFIR"
BASE COUNT         1751 a          540 c          658 g         1760 t
ORIGIN      
        1 atatattata tgtataaaat attataattt aataatttaa ccaaggagga aaaatatgag
       61 ctcagaacat aaaagtaaat tgtctttaac agcgcttgta ttaatgattt tcacatcagt
      121 atatggtttt gctaatatgc caaaggcatt ttatttaatg ggatatagtg ctattccatg
      181 gtacataata tcagcattag ctttctcaat tccatatgct tttatgatgg cagaatatgg
      241 tgcagcattt agaaaggaaa aaggaggaat atactcatgg atggctaaat ctgtaggccc
      301 taaatatgcc ttcatagtta catttatgtg gtattcatca aatttaatat ggttagttag
      361 taactcatca tcaatatgga ttcctttctc aaatgttata tatggaaaag atactacagg
      421 aacatggtct ttacttggat taagcgttcc acaaacaatg gctgtattag gatcaatact
      481 tataatagtt ataacttatg cagcatctaa aggattaaag agtataacaa aggttacttc
      541 aattggagga actgttgttg cattatcaaa catagtactt attttaggag caattattgt
      601 ttttgtaagc aatggattta aaccagctca gataataaat ggtgctgcct ttgttcatgc
      661 acaaaatcca tcgtatcaat cgccaatagg tgtattagga tttttagtgt ttgcagtgtt
      721 tgcttatggt ggactagaag cagtaagcgg attagttgat gaaactaagg atgctaaaaa
      781 gaattttcca aagggattaa tgattgcatc tataataata gccgttggat atgctgttgg
      841 aatactttgt gttggattat ttacaaattg gcaagaagta ttatcaggag ataatgttaa
      901 cttagcaaat gtatcatata tagtaattca aaacttaggt gtaaaattag gacaagcttt
      961 tggcatgagt actaatgctt cacttcaatt aggagcttgg tttgcaagat atataggtct
     1021 ttcaatgatg ttagccttaa tgggagcttt ctttacatta agttatgcac caattaaaca
     1081 attaatagaa ggaacaccta aagaaatctg gccaaaaaaa tggacaattt taaatgaaaa
     1141 taacatgcca gttaatgcaa tgtgggttca atgtatagtt gttgttatat ttatattaat
     1201 agcttctttt ggtggagaat ctgcaaataa attctttagt tatttaatat taatgggtaa
     1261 tgtggccatg acaataccat atatgttctt atctggagct tttccagcat ttaaaaagaa
     1321 aaagcatata gaaaaaccat ttattatgta taagtcatat aaatcatcat tgatttggtc
     1381 tataatagta acatttacaa ttggttttgc aaatttattt acaataataa agcctgttat
     1441 tcaagataag gattatacag ctatggcatt ccaaataact ggaccgataa ttttctctat
     1501 aattgctttt atactttatc ataattatga gaagaaaata aaaaataaat aatttaaaac
     1561 aacatctttt taagatgttg tttttcttat attttcttaa tatggattct tattgaattt
     1621 atgtagtata attattttaa aaataataaa atggagagtg tagcatatga agaaaaaaac
     1681 attaattatt ctaagtatta tatttatagt attactagga ataggttttt atttttttag
     1741 taaccaatct acatataaag tcataattaa taataatact aataaagaaa taagcgggtt
     1801 acaaataagt tataacaata ataagaagaa tattagtata ccaagcataa aagcaggtga
     1861 ttcagaggaa gtaaatgtaa aatcaaatga aaatggttct ttagttatgt attatacaga
     1921 tattttagga gattctcata aagaaatatt gtcgggttat tttcaaaaag gggctcaagg
     1981 aaaaatagta gtaaatatta catcggtaaa tactttgggt atatatggaa tggaaatagc
     2041 ttcacctgaa gcagagaata tactaaataa taagtaaact atgaaaggaa gtgaattaaa
     2101 aagagaactg ctctcttttt gtatttatgg aaaacattat atataatatt ttatatataa
     2161 taatatatag ctttttaggg tggtgctgtg aagttatata ttgttatata ttagatcaca
     2221 agtttacaaa tagaggattt ttaaatgggc cattatgccc tgtatacgga tttggagctt
     2281 tttttgtttt aacattttta gatttgtata aaagaaattt aatattagta tttatattag
     2341 gaatagtaat tacatcaatt ttagagtatg taacatcttt tttattagaa tcgatattcc
     2401 atactaaatg gtgggattat tcaaaacaca agtttaatat tcacggaaga gtgtgtttac
     2461 ttaattctac gctatttggt atattatcag ttatattagt tgaatttata aatcctatta
     2521 tagtagattt cgtaagtaaa tttccaaaaa agataatttt tcttataaca ataatcttat
     2581 taactctaat tgccattgat ttaatagtaa cagtacaagc tatgattaaa ttaaatgcaa
     2641 atttacacaa tcttcaaaat ttatctaaag aatttgaaaa acttggaatt tcttttatag
     2701 ataagacaga gaaagaatta gaaattacta ttagtaaaat taaacatgaa tccaatttaa
     2761 aagaagaact tttaaataaa gttcattcta taagaagaaa taatttactt gaaaaaaggc
     2821 ttttaaaagc attccctaat atgaaacata agagatacaa tgaactatta caaaaattaa
     2881 gaaaatatta taaattaaaa taaaggaagc caattaatgg cttcctttat tttaattgta
     2941 tttgtcattt gataacactt ctttttttac taatactcca ttttcataag tttccttata
     3001 agttgttgct gatttaggtc catgttgatc tacagatata actatttctt ttcctggatc
     3061 ttcttttata ccccaaatgt tagacttaac ttgtccagaa gaagttatat aagtttgaac
     3121 aataactgga ttattcatat tattttgaaa cttaaagtca atagatccac ttgccatagt
     3181 agcatctcta cctataggag cataagttac tggaaggcta tgaggagctc tctcaactat
     3241 ttttaagtca gcatataata ctgagttata aagagttgta actccttgac atattccgcc
     3301 agctaatata tctactactt tgtttccaat ataaccaggg gcataagtat aaccattact
     3361 ttcagttcta tcttctcctt ctttatcagc agaaaaaata tctcctggca ttaatacggt
     3421 accattaagc ttgcttaaat aattgctaat gtttttagta cgacctcctg tcaaactagg
     3481 aagtttagta gtatatgagc ctaaaataac catgttatta acagtttctt gagttaattt
     3541 aggttttatt tcttctacag gtatattaat agtacaattt agactatcat gactattatt
     3601 taaatcattg actatttcag aagtaagctt ttctaagtta agcttgtacc caatttgttc
     3661 tttagttgct gatatagaat catttgaaaa agaaatagtt ggttcaacct tttctttatt
     3721 gattttatta cttatttcag aaagtttttc acttaaaacc tttgaatttg attcaggttt
     3781 aaactgaaaa tatttaggat tagactttaa ctttaatctt tctattgtat tagaaaatat
     3841 attgctattt tttccgaaag aaaaaatttg attagataaa tcattaatat caaagaaaaa
     3901 tcctaaatct tttgctgaaa aagtttcatt aaaattgtct ttataattaa atgttatttt
     3961 aaaattattt aataaatcag agtagtgctc atttaatttt tcctttaaat ccttctcatt
     4021 taaatttgac acattaatgt tttcaacata aattccttta taagtaacat ttttactaga
     4081 ataagtttta aataaaacta cagaaattaa aattaagaat aaagctataa gtactagtga
     4141 aatttgtagt gctacctttt ttgaatttaa tttttttatt ctcttacttc tcacctttat
     4201 tacctcaact attattaatt gatattataa aattatacaa gtataattta acaaataaat
     4261 ataaataaaa tgtgtagatt ttgtataatt tgctttattc aaatactttt aattatgtag
     4321 taaataaatt ttatgtttag tagattttat attaaccttc tttaattata atataaaata
     4381 ttgaaaaaat atacaactct gaggtatttt tcatattata tttaataagc tataaattaa
     4441 aagaggagtt atgtttaaaa aaattacctt aaaagataaa tctatttact acaaatatat
     4501 agataaaaac aaatttttaa gttgtgaata ttcttttact acacttttta tgtggaaaga
     4561 ctttaataat atagaatatg atatagtaaa taatattttt attattagaa aatatgacaa
     4621 aattaacggt aaaattttca tgcaaccttt aggagatata gatgatgatt ctttaataaa
     4681 tattattgat tatttagaat ttataagaa
//
LOCUS       DAEVGI010000164         4674 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEVGI010000164 DAEVGI010000000
VERSION     DAEVGI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.165"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..521
                     /locus_tag="K8M77_002227"
     CDS             <1..521
                     /locus_tag="K8M77_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086690.1"
                     /translation="KRMISYLKNSFSKIFLIKKKENKLSSKMEYTNFNKKEVNIKSTN
                     SNILEEVAIEKLPVNYKIETTSHCLNSESKLPFEIKIPKNFKGRRKIPKVDCDLDEIN
                     FLNSNDYSLEDLKKDFRDLKLNSDFIKNINSDSLESNRDKSLEERVCNLEKKINELEE
                     FFLEIFQAMSNK"
     gene            550..1382
                     /locus_tag="K8M77_002228"
                     /pseudo
     CDS             550..1382
                     /locus_tag="K8M77_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590636.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1468..2082)
                     /locus_tag="K8M77_002229"
     CDS             complement(1468..2082)
                     /locus_tag="K8M77_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBI7086691.1"
                     /translation="MEIYTIGHSNYDLSRLVDMLNKYNINCVVDIRGIPYSKYNVQYN
                     KETIQKTLNDLGFTYIYMAKEFAAKRDSKISYNKEGYADFHKVINEESFKNGIERLKV
                     GISKGYRIALLGAMQEPVRCHRSILVGRELEKYGFKVNHILHDYSICDQREIENMLLD
                     KYFKDRGQITIDSLIGNEKSEEEMIEEGYDLANKEIGYRMEKLK"
     gene            complement(2310..2465)
                     /locus_tag="K8M77_002230"
     CDS             complement(2310..2465)
                     /locus_tag="K8M77_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI7086692.1"
                     /translation="MADRTLKCKDCGADFIFTEGEQAFYKEKGFENDPVRCLDCRRAR
                     KAQRNNR"
     gene            complement(2641..4299)
                     /locus_tag="K8M77_002231"
     CDS             complement(2641..4299)
                     /locus_tag="K8M77_002231"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBI7086693.1"
                     /translation="MSYENLANLIFPNIDKTPEYYFEKYPKRDLKEGAKVLRYAPSPT
                     GFQHIGGVFASLINERLAHQSGGIFYLRIEDTDQKREVEGAIDDTIKTMHNFGMDFDE
                     GITGENSEKGAYAPYKQSQRADIYRAFVKDLLRKGLAYPCFMTSEELEALREKQIAEK
                     LTPGCYGEFAKYRDLSPEEAIKRIEAGESYVIRMKSPGNPEKRVVAHDMIKGEVSFPE
                     NLQDVVIIKGDGLPTYHFAHAIDDTLMRTTHVIRGEEWLSSLPIHLQMFEVLGVEAPK
                     YAHIPTIMKMDGSSKRKLSKRKDPESAVSYYSEKGYPSQSVIEYLLNIINSAFEEWRA
                     ENPDADYHDYKVELDKMSKSGALFDLVKLNDVSKDVICKMKPEVVYDLYTNWAKEYDK
                     EMYDLVTSKEAMMKEVFNIDKEGPKPRKDFAKWDEVREKIFYFFDELFDKETANDVEL
                     PKTLELEEAKRIIEAYEKAYNFNTDKDTWFSDLKEVAVELGYATDRKKYKKNPEEYKG
                     MVSDVAGAVRAALTHRANTPDLYTIMQIMGEEAVRERFKKFLAL"
     misc_binding    complement(4354..4579)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1557 a          790 c          533 g         1794 t
ORIGIN      
        1 ttaaaaggat gatttcatat ttaaaaaata gtttttctaa aatattttta attaaaaaga
       61 aagaaaataa actttcttct aagatggaat atactaattt taataagaaa gaggtcaaca
      121 taaaatctac aaatagtaat atattagaag aagttgctat agaaaaactt cctgtgaatt
      181 ataaaataga aacaacttct cattgtctta acagtgaaag taaattacct tttgaaataa
      241 aaatacctaa aaattttaaa ggtagaagaa aaattcctaa agtagattgt gacttagatg
      301 aaataaattt tttaaattca aatgactatt ccttagaaga cttaaaaaaa gactttagag
      361 atttaaaact taattcagat tttataaaaa atataaactc tgattcccta gaaagtaata
      421 gggacaaatc tttagaagaa cgtgtatgta atcttgaaaa gaaaataaat gaacttgaag
      481 aatttttctt agaaattttt caagcaatgt caaataaata atcactaaaa agtggatata
      541 tcaaatttct atcttccacc ttcaaaaaaa ttcaaacttt ttaccgttga aggtgctact
      601 gccgctatgt gctatatttt tgattcctta atagcaaatg aattgcttcg aagaaaagat
      661 aaaatagcta taatgacacc tatttttact ccttatttag aaattcctca aatccctagg
      721 tatgatttag aaagtatata tattcatgct agtgaggtaa atgaggaagg gagctatact
      781 tggcagtatc cagagggtta ataagtgaac tgcttagtga taaaaaggaa atggtaaata
      841 aaagatatga atcactatca acagagccag aaaaacttcc atttattgat agactagttg
      901 ctgatagtcg ccaagtagca cttaatcaca ctgctggtct ttcaacacct caacaggtac
      961 aaatggcctt cttttctgca ttctctttag tggataaaga aaatacatat aaaaatcaaa
     1021 ctattgatat atgtcataga cgtcaaagat tattatttga aggactagac ttgccaatta
     1081 aaaaaaatcc atatgatgca tcatattata ctgaatttga tttattgcaa tgggctctta
     1141 aaaattatgg tgctgaattt gcaaattact tagaatcaaa ttataaacct gtagatgtat
     1201 tatataaatt agctgaagaa tcacctattg tcttattaag cggtggtgga ttccaatgtc
     1261 ctgaatggtc agtaagaata tctttagcta atttagatga tgatgcttat tcagaaatag
     1321 gaactgtact tagaaaaatc cttgaggact ttgttcataa ttggaaatct tcaactaaat
     1381 aaaatattat ggtaacctaa ctttatttat tataattagc attaaacttc aattaataaa
     1441 agctaaaata gtagttattc taaactacta ttttagcttt tccattctat atccaatttc
     1501 cttatttgct aaatcatatc cttcttctat catttcttct tcactctttt catttcctat
     1561 aagtgaatct atggtaatct gccctctatc cttaaaatat ttatctaaaa gcatattctc
     1621 tatttctctt tgatcacata ttgaataatc gtgaagtata tgattaacct taaatccgta
     1681 tttttctaat tctcttccga ctaatatact tctatgacat cttacaggtt cctgcatagc
     1741 ccctaataaa gctattctat atcctttact tattcctact ttaagtctct ctattccatt
     1801 tttaaaagat tcctcattaa taaccttatg aaaatcagca tagccttctt tattgtaaga
     1861 tattttgcta tctctctttg cagcaaattc ctttgccata tagatatatg taaatcctaa
     1921 gtcatttaaa gttttttgaa ttgtttcttt attgtattgc acattatatt tagaataagg
     1981 tattcccctt atatcaacta cacaatttat attatattta tttagcatgt caacaagtct
     2041 acttaaatca taattagaat gtcctatagt atatatctcc atataaaaca cctccaaaat
     2101 taattataat actaaactaa gatcaatatt gattttaact cttttagaaa atggtttatt
     2161 ttaaagtttc caacctctat ttaataacaa tatagatttt tcactatata ttgaaaatta
     2221 tacatacttt caatttagga agaatttttt attataaaaa taaaaaaagg ttgtaagact
     2281 aatcttacaa ccttataata aacaaattat tatctattgt ttctttgagc ttttcttgct
     2341 cttctgcaat ctaagcatct tactggatca ttttcaaatc ctttttcttt gtagaaagct
     2401 tgctctccct cagtgaatat gaaatctgct ccacagtctt tacattttaa tgttctatct
     2461 gccattttga catcctcctt aaatttaaag atttaaatgg attaataaaa attttcttta
     2521 agtttaagga aaattttatt catcttttaa agctttttct gttatgttct aattatacca
     2581 ctaaaaaatt aacattgcaa gctttttatt agcattgtgt taaaacctct taaaatcaaa
     2641 ttatagagct aagaattttt taaatctttc tcttacagct tcttctccca taatttgcat
     2701 tattgtataa agatctggag tattagctct gtgagttaaa gctgctctta cagctcctgc
     2761 aacatcagaa accattcctt tgtattcttc tgggttcttt ttatatttct ttctatcagt
     2821 agcatatcct aattctacag caacttcttt taaatctgag aaccaagtat ccttatctgt
     2881 attaaagtta tatgcttttt cgtaagcttc tattattctc ttagcttctt ctaattctaa
     2941 agtttttggt aattcaacat cattagctgt ttccttatca aataactcat caaagaagta
     3001 gaatattttt tctctaactt catcccattt agcaaagtct tttcttggct ttggaccttc
     3061 tttatctata ttgaatactt ctttcatcat agcttcttta gaagtaacta aatcatacat
     3121 ttctttatca tactctttag cccagtttgt atataaatca taaacaactt ctggtttcat
     3181 tttacagata acatctttac ttacatcatt taatttaact aaatcaaaca aagctccact
     3241 cttactcatt ttatctagtt caactttgta atcatgataa tctgcatctg gattttctgc
     3301 tctccactct tcgaaagctg agtttattat atttaaaaga tattcaataa ctgattgaga
     3361 tggatatcct ttttcgctgt aataacttac tgcactttct ggatcttttc tctttgataa
     3421 ttttcttttt gaacttccat ccattttcat tattgttgga atatgagcat acttaggtgc
     3481 ttctactcct aaaacttcaa acatttgaag atgtattgga agtgatgata accattcttc
     3541 tcctcttata acatgagtag ttctcattaa tgtatcatct attgcatgag caaagtgata
     3601 agttggaaga ccatcacctt ttattattac tacatcttga agattttctg ggaatgaaac
     3661 ctctcctttt atcatgtcat gagcaacaac tctcttttca ggatttccag gtgacttcat
     3721 tcttataaca taactttcac cagcttcaat tctctttata gcctcttctg gtgataaatc
     3781 tctatattta gcaaactctc cataacatcc tggagttaat ttttctgcaa tttgtttttc
     3841 tcttaaagct tctaattctt cagaagtcat aaaacatgga taagctaatc cttttcttaa
     3901 aagatcttta acaaaagctc tgtatatatc agctctttga ctttgtttat atggtgcata
     3961 agctcccttt tctgagtttt caccagttat tccttcatca aaatccattc cgaagttatg
     4021 catagttttt atagtatcat ctatagcacc ttcaacttct ctcttttgat cagtatcttc
     4081 tattcttaag tagaatattc caccactttg gtgagctaat ctttcattta ttaatgaagc
     4141 aaaaactcct cctatatgtt gaaatccagt tggacttggt gcatatctta atactttagc
     4201 cccttctttt aaatctctct ttggatattt ttcaaaataa tattccggtg ttttgtctat
     4261 gtttgggaat attaaattag ctaaattttc ataactcatt cttttcactt tcctttctaa
     4321 atttatgtaa taaaaaaagt ccctcgtcct aaaaattagg acgaaagact taacttccgc
     4381 gttaccacct aaattagtta gctaagctaa ctcactcata atcaattaag ggaattatcc
     4441 ctagacctac tccttaaaat ttaagatttt cagtctaagc tccgaagctt ctttctataa
     4501 gattattttt taggctctca ccctccctaa atcgctttaa aataattact tatgtactct
     4561 tcttcatcat agcaataaaa tatttcattt taaataacaa actctattat atcagattct
     4621 ttaaagttgt aaacaaaaat attaccactc ttaaatataa ttatttctta agtt
//
LOCUS       DAEVGI010000165         4622 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEVGI010000165 DAEVGI010000000
VERSION     DAEVGI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.166"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            2..77
                     /locus_tag="K8M77_002232"
     tRNA            2..77
                     /locus_tag="K8M77_002232"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Lys,seq:ttt)
     gene            218..1282
                     /locus_tag="K8M77_002233"
     CDS             218..1282
                     /locus_tag="K8M77_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBI7086694.1"
                     /translation="MKGIKKILSLGLLLVFSFSMVSCGGSKENTLEDTVVVTSKYPDE
                     VNSYIAEEFKKETGISVKYEVKDEIKEDDFKNSNTDIILGGNSELYKKMASDNILKGY
                     KTSWYSDVDDNYRDKDGYWYSIFRNPMVVAYNKANLAANLVPKSLADLKNGNLANKLL
                     MVNSNNDYTKYFISATASYLTKEANNDDNIGNTFLQGVKLNVATFFNNYDELFTALDT
                     KETPIGILPLDVLNKKIKDNANITRIDFEEGVPVITECAGILKSAPNPNASELFMEFV
                     AGPKIQLELAQKFNIMPTLPVAIKYSPDWIKNFKTLDIENNVVLENEDKWVQFFNGVV
                     KPEVPAKTTNNPVIKGKKKS"
     gene            complement(1357..1659)
                     /locus_tag="K8M77_002234"
     CDS             complement(1357..1659)
                     /locus_tag="K8M77_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086695.1"
                     /translation="MLVRCIDNSLCSSLTFGKEYVVIEEGDKYYVVVDDRNKEITTKK
                     QRFEVIEDSDLAKKAKATINELNFQINNEFKDIKDFKVRTNSKGEIKEVIIKFKYE"
     gene            complement(1778..2533)
                     /locus_tag="K8M77_002235"
     CDS             complement(1778..2533)
                     /locus_tag="K8M77_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBI7086696.1"
                     /translation="MNKRKWVTIGILPIMWLIYFLFEFLTGRIEKNSETLMMLFLIIP
                     FALVGYLVYVLANKYKDGFSKKTLLWIFMILMILDQGIKFIIHKWFFNDHFNIIGNFL
                     TFQPIINTDGSWLNVRFGTGLDFGFLIILNLIALIIFFECYRYYVHNGHKDFNADMCI
                     VFIMAGALCSLIDKVFYGGSLDFIGISNLFIADFKDIYINLAILFFILCIYFNDYWKD
                     DSTSTLKDDLASVKRFFIFAKNDLLVNILKLKK"
     gene            2750..3553
                     /locus_tag="K8M77_002236"
     CDS             2750..3553
                     /locus_tag="K8M77_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBI7086697.1"
                     /translation="MTDIIREYYNKNSKNEWLRLNDPYNRLELFSTMYMIKEYFPQEG
                     KILDIGSGPGRYSIELLKRGYEVSLMDLSEKSIDMAKRNIESMGLKANDYICGDALYL
                     DFIKDNTFDGILLMGPMYHVQSKEDRIRILENCMRILKPGGIILIAYINSLGVLKVGL
                     SDFPQEYKDINKIYDLFDEKGFSQEESFTETYFTVPEKAISEVNEVGFNILSRAGAES
                     FLSGQAFYMTKYYLEDKEIYFNLLKVATEKCEDERFRDSTEHLIIVAKK"
     gene            3742..4542
                     /locus_tag="K8M77_002237"
     CDS             3742..4542
                     /locus_tag="K8M77_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBI7086698.1"
                     /translation="MLTIFAVSDSIGETAQQVAMAAASQFKDNAEVKRIPYVKSLEDV
                     EDLIKTIEDCEACMIVSTIITVNVREYLTQKCIEKNINIINVLGPIINVASTILNKYP
                     DYNPGAMWNTDETYYKRIEAMEFAMQYDDSKDYRGLKNADVVLVGLSRTSKTPLCMYL
                     ANKGIKAINIPLVPEVGVPEELYEIDKKKIFGLTINPLQLIEIRKRRLDKFHRISADI
                     EYASDSRILEEFEFADKILRKIGCRTIDVTQRAIEDTALIIMEKLGVK"
BASE COUNT         1854 a          518 c          688 g         1562 t
ORIGIN      
        1 tgatttgcta gctcagtcgg tagagcacgt gacttttaat cacggtgtcc agggttcgat
       61 tccctggcag atcaccaaaa acctctctaa taagagaggt ttttttgtat ataaaaatat
      121 attatataaa ttagtggtaa aataatttat acttaagtta gttaagaatg taaaaaagag
      181 ttttaattat atttttatag tatttgggag gagagtaatg aaaggaataa agaaaatatt
      241 atcattaggt ttgcttttag tattttcatt ttcaatggta tcttgtggtg gatcaaaaga
      301 aaatacttta gaggatacag tagtagtaac aagtaaatat cctgatgaag ttaatagtta
      361 tatagctgaa gaatttaaga aagaaactgg aatatcagtt aaatatgaag tgaaagatga
      421 aataaaagaa gatgatttta aaaattctaa tactgatatt atcttaggtg gaaatagcga
      481 attatataaa aaaatggctt cagataatat tcttaaagga tataaaacaa gttggtatag
      541 cgatgtagat gataattata gagataaaga tggatattgg tattcaatat ttagaaaccc
      601 tatggtagtt gcttataata aagctaactt agcagcgaat cttgtaccaa agagtttagc
      661 tgatttaaaa aacgggaatt tagctaataa attattaatg gtaaattcca ataatgatta
      721 tacaaagtat tttatatctg ctacagcttc ttatttaact aaagaagcaa ataatgatga
      781 taatatagga aatactttct tacaaggtgt aaagttaaat gtggctacat tttttaataa
      841 ttacgatgaa ttatttacag ctttagacac taaagaaact ccaataggaa ttttaccttt
      901 agatgtttta aataaaaaaa ttaaagataa tgctaatata acaagaattg attttgaaga
      961 gggtgtaccc gttataactg aatgtgcagg tatattaaaa tcagctccta atccaaatgc
     1021 ttcagaacta tttatggagt ttgtagctgg gccaaagatt caattagaac tagctcagaa
     1081 atttaatata atgcctacat tacctgtagc aataaaatat tctcctgact ggattaagaa
     1141 ttttaaaact ttagatatag aaaataatgt tgttcttgag aatgaagata aatgggttca
     1201 attctttaat ggtgttgtta aaccagaagt acctgccaag acaactaata atcctgttat
     1261 taaaggtaag aagaaatctt aatacatatt aaaaaagaga tattatgttt aaaacataat
     1321 atctctttta tatttaaagt gtgaattgta aatagcttat tcgtatttaa atttaatgat
     1381 aacttctttg atttctcctt tagagttagt tctaacctta aaatctttta tatctttaaa
     1441 ttcattgtta atttgaaagt taagttcatt tatagtagct ttggcctttt tagctaaatc
     1501 actatcttcg ataacttcaa atctttgctt tttagttgta atttccttgt ttctatcatc
     1561 aactacaaca tagtatttat cgccttcttc aattacaaca tactccttac caaaagttag
     1621 gcttgagcat aatgaattat ctatacatct tactagcata gctactctcc tctcaattaa
     1681 tatattaaat tctctacacc tttaatatat cataataaga ggctttgtcc attataaatg
     1741 gataattaga ttaaatttgt gatatcttac aaatatacta tttcttaagt tttaatatat
     1801 ttactagtaa atcattttta gcaaaaataa agaatctttt tactgaagca agatcatctt
     1861 ttaaggtact agtgctatca tctttccagt aatcattgaa gtatatacat agaataaaaa
     1921 ataaaatagc taaatttata tatatatctt taaaatcagc tataaataaa ttgcttatac
     1981 ctatgaagtc taagcttcca ccataaaaaa ccttatctat taaagaacat aaagcaccag
     2041 ccattataaa tactatacac atatctgcat taaaatcttt atggccatta tgtacataat
     2101 atctataaca ttcaaagaat attattaatg ctattaaatt taatattata aggaatccaa
     2161 agtctaatcc agttccaaag cgaacattta gccaagagcc atctgtattt ataattggtt
     2221 ggaaggttaa aaaattacct ataatattaa aatgatcatt gaaaaaccat ttatgtataa
     2281 taaattttat tccttgatct aaaatcatta gaatcataaa tatccataat aatgtttttt
     2341 tgctaaatcc atctttatat ttatttgcta aaacatatac gagatatcct actaatgcaa
     2401 aaggaattat taaaaacaac atcataagag tttcagaatt cttttcaatt cttccagtta
     2461 aaaactcaaa taagaaataa atcaaccaca ttataggcag tatccctata gttacccatt
     2521 tccttttatt cattactacc tccctataag atataccatt agaatattta attaaaataa
     2581 caattaactt ttataaatat taaagagata tctataactt tagtctataa tagtgtcatt
     2641 aattaaacta aatcaatttt aacatagtta attattacaa ttaaagagga tatatagaat
     2701 tttaaaagaa tatataaatt atcacacata ataaatgacg aggtgtttta tgacagatat
     2761 tataagagag tactataata aaaatagtaa gaatgaatgg cttagattaa atgatccata
     2821 caatagatta gaattattta gtactatgta catgataaaa gagtattttc ctcaggaagg
     2881 aaaaatatta gatataggct caggaccagg aagatattca atagagctat taaaaagagg
     2941 atatgaagtt agtttaatgg atttgtcaga aaaatctata gatatggcaa aaagaaatat
     3001 agagagtatg ggattaaaag caaatgatta tatttgtgga gatgctttat atttagactt
     3061 tataaaagat aatacctttg atggaatact tcttatgggt cctatgtatc atgttcaatc
     3121 aaaagaagat agaattagaa tattagaaaa ttgtatgaga atattaaaac caggtggtat
     3181 tatattaata gcttatataa attctttagg ggtattaaag gtaggtcttt cagactttcc
     3241 acaagagtat aaagatataa ataagatata tgacttattt gatgaaaaag gatttagtca
     3301 ggaagaatct tttactgaaa catattttac tgttccagaa aaggctattt ctgaggttaa
     3361 tgaagtaggt tttaatatat taagtagagc cggggctgaa agctttttaa gtggacaagc
     3421 tttttatatg actaaatatt atttagagga caaggaaata tattttaatt tattaaaggt
     3481 tgcaactgaa aaatgtgaag atgaaagatt tagagattca acagaacatc ttataatagt
     3541 tgctaaaaaa taatagtatt ttaaagatta aatagtttat ttatataaaa tagtaactat
     3601 gagaattaat ttaaatctat ggcttaaaag ctctagtagc tataaaatat aaaaactaaa
     3661 agggaaaaga gagtaaatta cattaaaaaa acaacaataa taatatagtt gtttttagat
     3721 gaaaagagag gagcaatttt tatgttaaca atttttgcag tttcagattc cataggagaa
     3781 acagctcagc aggtagccat ggctgctgct agtcaattta aggataatgc tgaagttaaa
     3841 agaattcctt atgttaaaag cttagaagat gtagaggatt taataaaaac cattgaggat
     3901 tgtgaagcat gtatgattgt atctacaata ataactgtta atgttagaga gtatctaaca
     3961 caaaaatgta ttgagaaaaa tataaatata ataaatgtat taggtccaat aataaatgta
     4021 gcttcaacaa tattaaataa gtatccagat tataatccag gtgctatgtg gaatactgat
     4081 gaaacttatt ataagaggat agaagctatg gaatttgcta tgcagtatga tgatagcaaa
     4141 gactatagag gattaaaaaa tgctgatgta gttcttgttg gattatctag aacatctaaa
     4201 actccactat gtatgtatct tgctaacaag ggaataaagg caataaatat tccactagtt
     4261 ccagaggtag gtgtaccaga agaattatat gaaattgata aaaagaaaat atttggactt
     4321 acaataaatc cacttcaact tatagaaata agaaaaagaa gattagataa gtttcataga
     4381 atatcagcag atatagagta tgcttctgat tcaagaatat tagaggagtt tgaatttgca
     4441 gataagatac ttagaaaaat aggatgcaga accatagatg taactcaaag agctatagaa
     4501 gacactgctt taataataat ggaaaagtta ggtgtgaaat aattttttat aaacataatt
     4561 attttaaaac acaaaattta atagggataa atataaaggg gctgtataaa aatataataa
     4621 aa
//
LOCUS       DAEVGI010000166         4463 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEVGI010000166 DAEVGI010000000
VERSION     DAEVGI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.167"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..999
                     /locus_tag="K8M77_002238"
     CDS             <1..999
                     /locus_tag="K8M77_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI7086699.1"
                     /translation="KIINNNKTKFFLLLFLIILTGILCYLSITKGFIKTNYETVIDAY
                     KNFNGSQEHIIIKTSRVPRTLNALLVGGALGVSGLLIQALTKNKLASPSILGINSGAV
                     FILVIAITYIPQISNMGLACLSFIGALGATILVYILSGGLSGEVRTMDLTLGGAALSA
                     LLFSLTQGILYKNDIALEEIIYWMTGSVEGKKIDMILKFSPVIIVTIILTFFIWKKLN
                     VFSLGEEMARSLGIKTLYLKILIIAIVSVLSGVSVALAGPIAFVGLVTPSMVHKFIGS
                     DYKWLIPFSVFTGSSILLSADILSRFIIYPKEVPVGALTALIGGPFFIYIAKRRGK"
     gene            999..2021
                     /locus_tag="K8M77_002239"
     CDS             999..2021
                     /locus_tag="K8M77_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI7086700.1"
                     /translation="MINISKKTIKISILLLLITLLIFGISLSLGEVMISPIDVLKSIF
                     GIDTGFSSILVMNIRLPRVIIAFLIGGSLGLAGAILQGVVKNPLASPEILGISNGGAV
                     GALVFLTIFTNPNNNSLTTSILYMPIFTFIFSFLAVVIIFFICGKSTSTKKLIIIGVG
                     VSAIFKALTNILIINGPIVFMKEATTWLTGSIYGANWTHAAIISVIFFVFTIITVIFI
                     KDLNLHQLDDEIITVLGNKVYKSRIILLSISAALTAGAVTIGGGIPFVGLIAPHISRK
                     LVNSKFENLIPLSILIGGIITLLADFVSKWLFYPQELPIGIFTASIGAPYFIYLLIKN
                     RKSTNR"
     gene            2027..2797
                     /locus_tag="K8M77_002240"
     CDS             2027..2797
                     /locus_tag="K8M77_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086701.1"
                     /translation="MIKTNNLKLCYDEKIILDDINIEIEKGKITALIGPNGCGKSTLI
                     KSISKILTPKSGEVLMGDKNLLKMPSKELAKELAMLPQSSSAPEDLTIYDLVKQGRYP
                     YHNLLSFWSKKDEEIVMESIRKMGLIEEKDRTLENLSGGQRQRAWIALSLCQDTEVIL
                     LDEPTNHLDIKYQLEILNILKELNRKENRTIVMVIHDINHALRYADNIIALKDGNIFA
                     QGSKDKIINEDLIKNVFDIKCRIIDSPIDNGKMCVTYI"
     gene            3151..3666
                     /locus_tag="K8M77_002241"
     CDS             3151..3666
                     /locus_tag="K8M77_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBI7086702.1"
                     /translation="MYFNKVLGMKEFGKDEKVFYRVAIRAIILQGDKILMVKSNTGDF
                     KFPGGGVEKGETPEETLRREVQEETGYILNEVKEKFGVLIERDRRRRMGCTIFEMTSH
                     YYLCSVIEERGEQHLDKYEEELGFTPIWISLDEVIRENENILNQREKINPWVKRETFV
                     LKKIKKYLNEN"
     gene            complement(3816..4397)
                     /locus_tag="K8M77_002242"
     CDS             complement(3816..4397)
                     /locus_tag="K8M77_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBI7086703.1"
                     /translation="MNFWNDIFNSINGLAGKNHLLDSIMLFSSKRLPYILFATVIVLY
                     LIGIISKNKKARGIAVDTIVFTGINLILAVIIGHFFYAPRPFVNDPNAKLLYPHKPDS
                     SFPSGHSVGSMSIALGLNRYNKVLGTICVILSILMGISRVYVGHHYPQHVVASFLIVI
                     ILNVLYIKFLSKSVQKLYFSIEKHIPILNKLVK"
BASE COUNT         1677 a          469 c          731 g         1586 t
ORIGIN      
        1 aaaattataa ataacaataa gacaaaattt ttcttacttt tatttttaat aatattaaca
       61 ggaattctat gctatttaag cattactaaa ggatttataa aaactaatta tgaaactgtt
      121 atagatgctt ataaaaattt taatggttct caagagcaca taattataaa aacatctaga
      181 gttcctagaa ctttaaatgc tcttttagtt ggaggagcac ttggtgtttc tgggcttttg
      241 atacaagcat taactaaaaa caaacttgct tctcctagta tattaggtat aaattcggga
      301 gcagtattta ttttggttat tgctataaca tatattccac aaatttctaa catgggactt
      361 gcatgcttat cttttatagg ggcattagga gctactatat tggtatatat attgtcaggt
      421 ggactttctg gtgaagttcg aactatggac ttaacgttag gtggagcagc tttaagtgcc
      481 ttgcttttct ctttaactca aggaatatta tataaaaatg atattgcttt agaagaaatt
      541 atatattgga tgactggttc cgtagaaggt aaaaaaatag atatgatact taaattttca
      601 ccagtaataa tagttactat aatcttaaca ttctttattt ggaaaaagct taatgtattt
      661 tctttaggtg aagaaatggc aaggtcatta ggaataaaaa ctttgtattt aaaaatatta
      721 ataatagcta tagtatcagt tttatcagga gtatctgtag cacttgctgg acccatagca
      781 tttgttgggt tagtaactcc aagtatggtg cataaattta ttggttcaga ttataaatgg
      841 cttattcctt tctctgtgtt tactgggtcc tctatattat tatcagcgga tatattatca
      901 agatttataa tttatcctaa agaagtacca gtaggagcat taactgctct tataggagga
      961 ccattcttta tatatattgc taaaaggagg gggaagtaat gataaatata agtaaaaaga
     1021 ctataaaaat aagtatatta ttgttattaa ttactctttt aatatttggg ataagtttaa
     1081 gtttaggaga ggttatgata agtcccatag atgtattaaa aagtatattt ggaatagata
     1141 caggtttctc ttctatatta gtaatgaata taagactacc aagagttatt attgcttttt
     1201 taattggagg atctttagga ttagcggggg ctatacttca gggagtggta aaaaacccat
     1261 tagcatctcc tgagatatta ggtatatcaa atggtggagc tgttggagca ttagtatttt
     1321 taacaatttt cactaatcct aataataatt ctttaacaac atcaatatta tatatgccta
     1381 tattcacctt tatattttct tttttagctg tagttattat tttctttatt tgtggaaaat
     1441 ctacatcaac aaaaaagtta attataatag gtgttggtgt ttctgcaata tttaaagcat
     1501 taactaatat tttaataata aatggaccaa tagttttcat gaaagaagct acaacatggt
     1561 taacaggtag tatttatggt gcgaattgga ctcatgcagc tataatatca gtaatattct
     1621 ttgtttttac aataataaca gtaatattta taaaagattt aaatcttcat caattagatg
     1681 atgagattat aaccgttctt ggtaataagg tttataaaag tagaataata ttgttaagta
     1741 tatcagcagc cttaacagcg ggagctgtaa ctattggagg aggaatacct tttgttggat
     1801 tgatagctcc tcacatttca agaaaacttg tgaattcgaa atttgaaaat cttattccgt
     1861 tatcaatact tataggagga ataatcacat tattagctga ttttgtttct aagtggttat
     1921 tttatccaca agagttgcca atagggatat ttacagcatc tataggagct ccgtatttta
     1981 tatatctttt aataaaaaat agaaagtcta caaataggtg atgaaaatga taaaaacaaa
     2041 taatttgaaa ttgtgttatg atgaaaagat aatattagat gatataaata tagaaataga
     2101 aaaagggaaa attacagcat taattggacc taatggttgt ggtaaatcta ctttaataaa
     2161 gtctatatct aagatactta ctcctaaaag tggtgaagtc ttaatgggag ataaaaatct
     2221 tcttaaaatg ccatcaaagg aattagcaaa ggaattagct atgttaccac aaagtagtag
     2281 tgctccagag gatttaacta tttatgattt agttaagcag gggagatatc cttatcataa
     2341 cttattatca ttttggagca aaaaagatga ggaaatagta atggaatcta ttagaaaaat
     2401 gggactaata gaagaaaaag ataggacttt agaaaatctt tctggtggac aaagacaaag
     2461 agcatggatt gccttatctt tatgtcaaga tacagaagtt atacttttag atgaacctac
     2521 aaatcattta gatataaagt atcagctaga gattttaaat attttaaaag agcttaatag
     2581 aaaagaaaat agaactatag taatggtaat tcatgatata aatcatgctt taagatatgc
     2641 tgataatata atagctttaa aggatggaaa tatatttgcc caaggtagca aagataaaat
     2701 tataaatgaa gatcttatta aaaatgtttt tgatattaaa tgtagaatta tagattcacc
     2761 aattgataat ggtaagatgt gcgtaactta tatataaaat atagctttaa ttgcattaag
     2821 atttaaataa taagattttt aactagaaat atactaagct tttatatttt gttcaataat
     2881 attatgttta gtagggtata ttataaatag gttaaagaat attatattag taaaggtaat
     2941 acattaaaaa tagcttctta ataattatat aatttatttt tgtatattat tggcttttag
     3001 attattcaaa atggatttac atataaagta tttagatgta aaataaaatt ataatattaa
     3061 aatgcagatg attattaggg gagtttaggg aatgaactcc cttctttttt gtatttatgg
     3121 atatattaat ttcattaaag gggtgaagat atgtatttta ataaagttct agggatgaaa
     3181 gagtttggaa aagatgaaaa ggtattttat agagttgcca taagggcaat tattttacag
     3241 ggtgataaaa tacttatggt aaaatctaat acaggagatt ttaagttccc aggtggcgga
     3301 gttgaaaaag gggagactcc agaggaaacc ttaagaagag aagttcaaga agaaacagga
     3361 tacatattaa atgaagttaa agaaaaattt ggagttttaa ttgaaagaga tagaagaagg
     3421 agaatgggtt gtacaatttt tgaaatgacc tctcattatt atctttgcag tgttattgaa
     3481 gaaagaggag aacaacattt agataagtat gaggaagagc taggttttac tcctatatgg
     3541 atttctttag atgaagtaat tagagagaat gaaaatatac ttaatcaaag agaaaaaatt
     3601 aatccatggg tgaaaagaga aacttttgta cttaaaaaga ttaaaaaata tttaaatgaa
     3661 aattagtttt aaatataaaa atactttatc aaaaatttaa tatctttttg ataaagtatt
     3721 tttctttgta tagattttta atttaaagat tatatagtat agaaattaaa attcacttaa
     3781 aaattagctt tttatttaaa ctttagagat ttagattatt taactaattt atttaaaatt
     3841 ggtatatgtt tttcaattga aaaataaagt ttttgaacgc ttttgcttaa gaattttatg
     3901 taaagtacat ttagtataat tacaattaag aatgaagcaa caacgtgttg aggatagtgg
     3961 tgtccaacat atactctaga aattcccatt aatatagata aaattacgca aattgttcca
     4021 agaactttat tatatctatt taatcctaaa gctatactca tactaccaac tgaatgacca
     4081 cttggaaaag atgaatctgg cttgtgtgga tataatagct ttgcattggg atcatttaca
     4141 aaaggtcttg gagcataaaa aaagtgacct ataataacgg ctagaattaa attaattcca
     4201 gtaaatacaa tggtgtctac agctattcct ctagcttttt tatttttact tataatccct
     4261 attaaataaa gaacaatcac tgtagcaaaa agaatatatg gaagtctttt tgaactaaaa
     4321 agcataattg aatctaataa gtgatttttt cctgctaacc cattaataga gttaaaaatg
     4381 tcattccaaa aattcatatg tggttctcct taataatttc ttcttaagtt atatttataa
     4441 aattttatga tgcttgtaca caa
//
LOCUS       DAEVGI010000167         4379 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEVGI010000167 DAEVGI010000000
VERSION     DAEVGI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.168"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(331..2679)
                     /gene="dnaE"
                     /locus_tag="K8M77_002243"
                     /pseudo
     CDS             complement(331..2679)
                     /gene="dnaE"
                     /locus_tag="K8M77_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            complement(2995..4245)
                     /gene="dnaE"
                     /locus_tag="K8M77_002244"
     CDS             complement(2995..4245)
                     /gene="dnaE"
                     /locus_tag="K8M77_002244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBI7086704.1"
                     /translation="MGDKKFCHLHLHSGYSLLDGSGKIKPLIKRAKELGMESIALTDH
                     GVMYGCVDLYKEAKAEGIKPILGCEVYVVPKSRKIKSNDEDNKTYHLVLLVKNEKGYE
                     NLMKIVSVASIEGFYYKPRIDREYLKEHSEGIIALSACLGGEVQKAILNGNIDKAREA
                     ATFYRDTFKDGFYLELQNHGIEEQKKVNEVNIQLAKELNIPLVATNDVHYINQEDSKA
                     HDILMCIQTGKTVDDPNRRRYPSDQFYLKSPEEMWDMFDYIPEALENTLKIAEECNFD
                     YEFHVSKLPKFPLPEGVEPFEYLRKTCFDGLIDRLEELKPLKEKGIYDIDKVYEIGEN
                     NEKVKEDIERLEYELGVIKQMGYVDYFLIVWDFIKYANDNGIPTGPGRGSAAGALVAY
                     TLGITKIDPMKYSLLFERKLRCAV"
BASE COUNT         1306 a          881 c          493 g         1699 t
ORIGIN      
        1 ataataaatt tattcactta ttttaaaaaa attttatttt tttaatttta agaaaattta
       61 caaattaatt ttagcacttt taaaaaaaaa gtccactttt aaaaagactg ttactagtgc
      121 ttcatttaaa atatctccct caaactcaat aagtaatcgt taatacccat tctattaaaa
      181 aaatatttta tactctagtt ttccttctaa agtggacatt catttcctat tttatttagt
      241 tttaacaaat tttaagttta atattataag attaaatttc aatctttatg aaaacactta
      301 aacttttctt tcaactctga attataatta ttaaccttca actactttta catttccttc
      361 tccaaatttg ctaattaaaa aatctattac atctgtatct aaatcaaccc acatatctct
      421 gtttaatcta aaagatgctt tttcctttgc tgcaaatata taaactggag aatcaccttt
      481 atattgtgat aataaaactc ttaaaggctt aactatcata ttagcttttt ctctatcttc
      541 aacctttata tacacctttg aagaatttat taattctagt ggttgtatat cttcacaaag
      601 tatttttggc tcctcatctt cctttagaga aactcttcct cttattaaaa caaaggagtc
      661 ctcatttact agtgcattac atttttctaa ggttttaggg aatataacac attctaaata
      721 gccagttaag tcctctacct ttgcaaatgc cataagagta ttatttctag ttacctttct
      781 agaaacatta gtaagaatcc ctcctataat cactctagtt ccatcagtta aagaactgtc
      841 taagactaaa tcttctaatt caacattttt ttgctcattt aaattctttt gagcttctat
      901 tattttttct atagttattg atgtttgctc ctttaaaggt ttttcataat catctagtgg
      961 atgaccactc atataaagtc ctgtcatttc cttttccata gcaagcatat atttcttgtt
     1021 gaattccttt atatttggat atcttatttc taagtttgat tcactctctt cttgaagtgc
     1081 tccaaacaat gacatttgcc catctatatt tttctttctc tgagaatata ctccatccat
     1141 tattttttca aaaacagata gtaattgtga tctataaacc tttaagctat caaatgtacc
     1201 agcttttatt aaactttcta ctgatctttt atttacaata cttaagtcta ttttattgca
     1261 aaaatccata agggaagtga atttaccctt ttcatctctt gatttagcta tgttttctac
     1321 aacatttacc cctacatttt ttatagctcc cataccaaat cttatagtat cacccttaac
     1381 tgtaaactta gtataactct cgtttatatc tggtggtaat acctgaattc caagcttttc
     1441 tgcacttctt atatattcag ataccttatc actgttaccc ataacagagt taagcatagc
     1501 agctaaaaac tctactggat aatatctcat aagataagct gtctggaatc ctattacggc
     1561 ataggcagca gcatgacttt tgttaaatgc ataggatgca aaatccatca tagagtcaaa
     1621 tattttatta gctgcctcag ctgaaatacc atttcttaag caacctggaa cctctatatt
     1681 tccattttca tcttcaattc cataaatgaa gttctttctc tcttcttcca tgaccttatg
     1741 ctttttctta gacatagctc ttctaacaag gtcacttcta cccatggaat atcctgctaa
     1801 ttttctaaca atctccataa cctgctcttg ataaaccata accccatagg ttacatttaa
     1861 tatgttttca agctcaggaa ctatatattc tactgacttt ggatcccttt taccacttat
     1921 atatcttggt atttctgcca taggaccagg tctatataat gatattcccg ctatgatatc
     1981 ctctaaacag tctggcttaa gctctttcat gaaagaggtc attcctgctg actctaactg
     2041 gaatacccct actgtatttc cttcaccaat cattttataa acttctttat catcaaagtc
     2101 taaattatct aaatctatgt ctactcccct gttatacttg atcatatcca cagcatcacg
     2161 cataacagtt aaggttctaa gacctaagaa gtccattttt aataaaccaa gctcttcaag
     2221 tgttgtcata tcaaattgag ttacaatgga ttcatcattt ttttgaagtg gaacatactc
     2281 aactaaaggc tgagaagcta taactactcc cgcagcgtga gttgatgaat gtcttggaag
     2341 cccttctagc cttctactta catcaataag ttcttttact ctctcttcag tatcatatat
     2401 tgttttaagc tctggattta aatctaaagc cttatctatt gttactccaa gaactgtagg
     2461 tatttgttta gctattctat caacctctgc ataagtgtaa ttcatagctc tacctacgtc
     2521 cctaatacaa gctcttgctg ccattgttcc aaaagttata atctgtgata cattgtcgtg
     2581 accatacttc tcaacaacat agtctataac tctttgtctt ccctcataac agaaatcgga
     2641 atctatatct ggcatactta ctctctccgg atttaagaac aattagtgtt tatctacaag
     2701 ctctttatct tgtactctgg aagtttcctt cattttaatc aagtagtcat ttctacttca
     2761 gtttggacta tatcatcata ttgaatacat tcttttttct atgcataaaa tattacttat
     2821 attttggttt gctaaatata tttatattca atatgtcggg aactcgtgga aaagattatt
     2881 gcttagctac tcacttttct agtctctgaa cctttctagt acttttctgc tattcctaga
     2941 cttggctgct gattggcata tctttcgact tagcttccca gcaattcacc cgatttaaac
     3001 agcgcatctt aatttacgct cgaagagtaa gctgtatttc atagggtcta tttttgttat
     3061 tcctaaggta taggcaacta aggccccagc tgctgatcct cttcctgggc cagttggtat
     3121 tccattgtca ttagcatact taatgaagtc ccaaactatt aggaagtaat caacatatcc
     3181 catttgcttg attactccaa gctcatattc aagcctttct atatcttctt ttactttttc
     3241 attattctca ccaatttcat aaaccttatc tatatcatat attccctttt ccttaagagg
     3301 cttaagttct tctaatctat ctataagacc atcaaagcaa gtctttctta gatattcaaa
     3361 aggctctacc ccttctggca gtgggaactt tggaagcttt gaaacatgga attcataatc
     3421 aaaattacat tcttctgcaa tctttaatgt attttctaaa gcttctggaa tgtaatcaaa
     3481 catatcccac atttcttctg gtgattttaa atagaattgg tcagaagggt atcttcttct
     3541 atttggatca tccacagttt ttccagtttg tatgcacata agtatgtcat gtgcctttga
     3601 atcttcctgg tttatatagt gaacatcatt agttgcaact aaaggaatat taagctcttt
     3661 agctaactgt atatttacct cattaacctt tttttgctct tctattccat gattttgaag
     3721 ttctaaataa aatccatcct taaatgtatc tctataaaat gttgcagctt ctcttgcttt
     3781 atctatattt ccatttaaaa tagctttttg aacttctccc ccaagacaag cacttagggc
     3841 tattattccc tctgaatgtt cttttaaata ttctctatca attcttggtt tataataaaa
     3901 tccttcaata gatgctacag aaacaatttt cataaggttc tcatatccct tttcattctt
     3961 aacaagaaga actaagtgat atgttttatt atcttcatca ttgctcttta tctttctaga
     4021 cttaggcaca acataaactt cacatcctaa gataggtttt attccttctg ccttagcttc
     4081 tttatataag tcaacgcatc catacataac accatggtca gttaatgcta tactttccat
     4141 tccaagttcc tttgcccttt taataagtgg ctttattttt ccagaaccat caagcaaact
     4201 atacccagag tgtagatgca agtgacaaaa cttcttatcc cccatttcct tcacatcctt
     4261 tcatgtaata ttgtttctat aaaatttttt aatcatatat aaaatacttt ttttaagttt
     4321 aaatcttaaa aatcatctct atttttttat taaattaaac acttaaaaat tatgtttta
//
LOCUS       DAEVGI010000168         4360 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEVGI010000168 DAEVGI010000000
VERSION     DAEVGI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.169"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            96..431
                     /locus_tag="K8M77_002245"
     CDS             96..431
                     /locus_tag="K8M77_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086705.1"
                     /translation="MSKYKVGDELIIVTDPNQETKKLKDLSFLKKDGDFACLSWGIKI
                     VDVLYEKPNVTLTYKNVMGETNKVFAVCKNVENFDHEKVLEKALLKAFQNELTNITVI
                     KNSGLKTIL"
     gene            461..637
                     /locus_tag="K8M77_002246"
     CDS             461..637
                     /locus_tag="K8M77_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086706.1"
                     /translation="MAYCPFWSNEREKFKCYKECPMNEKNTGEECVFFMYLDKSFLLE
                     DECEKLNLVLSQVN"
     gene            complement(689..2161)
                     /locus_tag="K8M77_002247"
     CDS             complement(689..2161)
                     /locus_tag="K8M77_002247"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBI7086707.1"
                     /translation="MDYKFDVKNPRNLTMLVDFYELTMANGYFKNGIENKIAYFDMYF
                     RRVPDGGGYCIMAGVEQLIEYLSNLNFSEDDIAYLRSKELFCDEFLNFLINFKFTCDV
                     WAIPEGNPVFPNEPLVIVKGPVIQAQFVETMILLTINHQTLIATKANRICRAAKDKTV
                     MEFGSRRAQGYDGAIYGARAAAIGGCDSTACTIADQMFDIPAIGTMAHSWIQLFGDEY
                     EAFKAWAVAYPDNCTLLIDTYNVLKSGVPNAIKVFDEVLKPLGKRPKGIRIDSGDITY
                     LSKKCRQMLDEAGYPDCKIIVSNSLDEYIIRDVLSQGASIDAFGVGERLITARSEPVF
                     GGVYKLVAVEDEEGNIIPRIKISENEEKITNPGFKRIIRIFDNNTHTALADLILLNEE
                     EIDTTKPLEIFDPIHVWKRKTLTNYYVKDLLVKIFEDGKVCYKNPTVMEIRNYAQKEC
                     EKLWPEILRFENPHTYYVDLSNGLWNLKHNLLHRYSSIYK"
     gene            2314..4308
                     /locus_tag="K8M77_002248"
     CDS             2314..4308
                     /locus_tag="K8M77_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBI7086708.1"
                     /translation="MSNLDKYQNEAVRARSKSVLVIAPPGAGKTTVILNRIKYLLEER
                     KVKGIHVIVITFTKAAAENMKSRFKEMHKEGVVPFFGTFHGLFYKILLRNKDEIRLIE
                     SKDSYNIIRKVLSSYIEEVSDEKIKEVLNNISRKKVSSKDLEISMTYDIFDKCYEAYE
                     TFKNERGLLDFDDLQIKAVTLLKENPHILGYYRNLIKYILVDEFQDCDEVQLEFLRLI
                     NNEVFCVGDEDQSIYSFRGATPKCMINFEENFEGSEKVYLKYNYRSLKNIVEVSKDVI
                     KHNKERYQKEIIAFNENQGKIELKKPFNESEEANIIALKIEESKRLGNKYSDTAILYR
                     TNMESRSFIDAFIRKKIPFKLLDKEYNFFNHFICRDLIAYLRLSIDPFNKEDFNKIIN
                     KPFRYVSKSAIYGVMNSKKKENVFDILMNVEGVHPFQMRKFEELKSDIAYLNKLSLNS
                     AIDFILSDLEYSQYIREYSSKFKQNQEDLLDIVEEFKSSAMEFKSIITFLSHIESVEE
                     NLENLNSKGNIDSVILSTMHGVKGMQFNDVHIVNIVDETIPHKNSMENLEEERRLFYV
                     GITRAINNLYLYAPKSVRGKFKDPSIFLNNTNLDSQCNITDYGLKEGDAIRHNYFGIG
                     EILKIEKDTISIKFIQGENKKFSLRTLIENNLIEKIICEV"
BASE COUNT         1672 a          508 c          658 g         1522 t
ORIGIN      
        1 aaaaataata aaaaaatata agattgcaat actttactta aagttaaata agtggtaaac
       61 tttactatat aaataaatta cttaggaggt taattatgtc aaaatataaa gttggagatg
      121 aattaattat tgttacagat cctaatcaag aaactaaaaa gttaaaagat ttaagtttct
      181 taaaaaaaga tggagatttt gcttgtttat catggggtat aaaaattgtt gatgtattat
      241 atgaaaaacc gaatgttaca ttaacttata aaaatgttat gggagagact aataaagttt
      301 ttgctgtatg taaaaacgtt gagaattttg atcacgaaaa agttttagaa aaggctttac
      361 ttaaagcttt ccaaaatgag cttacaaata ttacagttat aaaaaattct gggttaaaga
      421 ctatattata attaatttaa gaagggattg agagcacaat atggcgtatt gtccattttg
      481 gtcaaatgag agggagaaat ttaaatgtta taaggaatgt ccaatgaatg agaaaaacac
      541 aggagaagag tgtgtattct ttatgtattt agataaaagt tttttacttg aggatgaatg
      601 tgaaaaatta aatctagttt tgagtcaggt aaattaataa gagatgcttc acattaatga
      661 agcatctctt atttttatat ataaataact atttatatat gctagaatat ctatgaagta
      721 aattatgttt taaattccat aaaccatttg ataaatcaac atagtaagta tgaggatttt
      781 caaatcttaa aatttctggc caaagttttt cacattcttt ttgagcataa tttctaattt
      841 ccattactgt tggatttttg tagcatactt ttccatcttc aaatatttta actagtaaat
      901 ctttaacgta ataattagtt aatgtttttc ttttccatac atgaatagga tcaaaaatct
      961 ctaatggctt agtagtatct atttcttctt catttagaag aattaaatca gctaaggctg
     1021 tatgagtatt attatcaaat attcttatta ttctcttaaa tccaggattt gttatttttt
     1081 cttcattttc gcttatcttt attctaggaa ttatattacc ttcttcatct tctactgcaa
     1141 ctaatttata aactccacca aaaacaggtt ctgatctagc tgttataagt ctttctccaa
     1201 ctccaaaggc atctattgaa gctccttggc ttaatacatc tcttattata tattcatcta
     1261 gagaatttga aactattatt ttacaatctg gatatccagc ttcatctaac atctgtctac
     1321 attttttaga tagataagtt atatcaccac tatctattct tattcctttt ggtctttttc
     1381 ctaaaggttt taaaacctca tcaaatacct taatagcatt aggaacacca gactttaaaa
     1441 cgttataggt atctattaat aatgtacagt tatcaggata tgctactgcc caggccttaa
     1501 atgcttcata ttcgtctcca aaaagttgaa tccaactatg agccatagtt cctatggcgg
     1561 gtatatcaaa catttgatca gctattgtac aggctgtaga atcacaacca cctatggcag
     1621 cagctcttgc tccataaatt gctccatcat atccttgagc tcttctagaa ccaaattcca
     1681 ttacagtctt atccttagct gctctacata ttctattagc cttagtagct ataagtgttt
     1741 ggtgatttat agtaagaagt atcatagttt ctacaaactg tgcttgtatt acaggtccct
     1801 taacaataac taaaggttca tttgggaaga caggatttcc ttctggaatt gcccaaacat
     1861 cacaagtaaa cttgaaattt attaaaaagt ttaaaaattc atcacaaaat aattcctttg
     1921 atcttaaata ggctatatca tcttctgaaa agtttagatt tgataggtat tcgattaatt
     1981 gctcaacacc agccattata caatagccgc caccatcagg tacccttcta aaatacatat
     2041 caaaataggc aattttattt tctatgccat ttttaaaata tccatttgcc atagtaagtt
     2101 catagaaatc aactagcatt gtaagatttc tagggttttt tacatcaaac ttataatcca
     2161 tataaaaatt atctcctttt cataagttat caataactat tgtaaccagt agttagaatt
     2221 attacaatat atatgttaat aaatattatg gtttactaga ttaattttag tcaataatta
     2281 aaataaattt tattaaatat taggagaatt ataatgtcta atttagataa atatcaaaat
     2341 gaagctgtaa gagcaaggag taaaagtgtt cttgttatag ctccaccagg agcaggaaag
     2401 accacagtaa tattaaatag aattaaatat cttttagaag aaagaaaggt taaaggaata
     2461 catgttatag taataacttt tactaaggcg gcagcagaaa atatgaaaag tagatttaaa
     2521 gaaatgcata aggaaggtgt tgttccattc tttggaacct ttcatggact tttttataaa
     2581 attttactta gaaataagga tgaaataagg cttatagagt ctaaggattc ttataatatt
     2641 ataaggaaag tattatcatc ttatatagaa gaggtaagtg atgaaaaaat aaaagaagta
     2701 ttaaataata tatctagaaa gaaagtttct tctaaggatt tagaaatatc aatgacttat
     2761 gatatttttg ataaatgcta tgaagcatat gaaactttta aaaatgagag aggactttta
     2821 gattttgatg atttacaaat aaaagcagta actcttttaa aagaaaatcc tcatatattg
     2881 ggatattata gaaatttaat aaaatatata ttagttgatg agtttcaaga ttgtgatgaa
     2941 gttcaattag aatttttaag acttataaat aatgaagttt tttgtgttgg agatgaggac
     3001 caaagtatat attcctttag aggagcaact cctaaatgta tgataaactt tgaggagaac
     3061 tttgaaggta gtgagaaggt ttatttaaaa tataattata gaagcttaaa aaatattgtg
     3121 gaggtatcta aggatgttat aaagcataat aaagagagat accaaaaaga aataatagct
     3181 tttaatgaaa atcaagggaa aatagaactt aaaaagccct ttaatgagtc tgaggaagca
     3241 aacataattg ctctaaaaat agaagaaagt aaaagattag gtaataaata ttctgatact
     3301 gctattttgt atagaacaaa tatggagagt agaagtttta ttgatgcctt tattagaaaa
     3361 aagattccct ttaagctttt agataaagag tataatttct ttaatcattt tatatgtaga
     3421 gatttaattg catatttaag actctcaata gatccattta ataaagaaga ttttaataaa
     3481 ataataaaca aaccatttag atatgtttca aaaagtgcta tttatggagt gatgaattct
     3541 aagaagaaag aaaatgtttt tgatatatta atgaatgtgg aaggcgtaca tccatttcaa
     3601 atgagaaagt ttgaggagtt aaaaagtgac atagcttatt taaataaact ttccttaaat
     3661 agtgctattg attttatatt aagtgattta gagtatagtc agtatataag agagtattca
     3721 tctaaattta agcaaaatca agaggattta ttagatattg tagaagaatt taaaagttca
     3781 gctatggaat ttaaatctat aatcactttt ctttctcaca tagaaagcgt tgaagaaaat
     3841 ttagaaaact taaattctaa aggtaatata gactctgtta tattaagcac catgcatggg
     3901 gtaaagggaa tgcagtttaa tgatgttcac attgtaaata tagttgatga aacaatacca
     3961 cataaaaaca gtatggaaaa tttagaagag gagagaagac ttttttatgt gggtataact
     4021 agagccataa ataatttata tctttatgca cctaaaagtg taagaggtaa atttaaggat
     4081 ccatctatat ttttaaataa tacaaatcta gatagccaat gtaatataac tgattatgga
     4141 ttaaaggaag gagatgccat aagacataat tattttggca taggtgaaat tttaaaaata
     4201 gagaaggata ctatttcaat taaatttatt caaggagaaa ataaaaaatt ttctttaaga
     4261 actttgattg aaaataattt aatagaaaaa ataatttgtg aagtttaaat gtgtagtaac
     4321 aaaggaaatt agagaattta aataaaaatt ttctttgtta
//
LOCUS       DAEVGI010000169         4324 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEVGI010000169 DAEVGI010000000
VERSION     DAEVGI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.170"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..879)
                     /gene="ytxC"
                     /locus_tag="K8M77_002249"
     CDS             complement(1..879)
                     /gene="ytxC"
                     /locus_tag="K8M77_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBI7086709.1"
                     /translation="MLLMTLCYEEEKNLIKDMQNIKNVLKSKGIIIGVSESISYGTHF
                     VKIYYGNSEFDENMRATIMLYISNVIYNVIVEHYREKEMLHYINENYFFLKHDEILDI
                     DLAINKILKGDQKICSDKDFYCLNKINDIIENIKEFILENDYINIEGFITFRMKNFLK
                     DIECIIDKVVEDYMIEKEYNEFIKLLKYFVDIQDCKLEEVNIIVQKNGSYEVKDSKGL
                     DIFKDFLNEITDAAEVGIINTEDIIISGLITNVPKKIKIYNEEYCINKEFIQTIKSVF
                     GERVETHRSYNNILKK"
     gene            1014..1709
                     /locus_tag="K8M77_002250"
     CDS             1014..1709
                     /locus_tag="K8M77_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086710.1"
                     /translation="MRTCFVDYRISELEEKKLKDLNFNIIKVPKHPKLYDAIDGHPDI
                     QINILNENTLLLAKDSSEELFNSIPKDLNIIKSSYNLQDKYPGNVILNAVNLKNDFIH
                     NLKFTDPKLIESVNGKNLINIKQGYSKCSIAVVSEKALITSDKGIYKALKPHGFDILL
                     IPSGDVSLPGLDYGFIGGTCGLISKNQMAFFGNLENHPYGNHIKNFLLKYDVEPIYLS
                     NGKLIDRGSILTI"
     gene            1964..2944
                     /locus_tag="K8M77_002251"
     CDS             1964..2944
                     /locus_tag="K8M77_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI7086711.1"
                     /translation="MFYLGIDAGGTKTSFMLINHLGEVLSTYTSGTCHIHQVGFDGFR
                     KGIQEGIDKICNEINICRDDFSYSFLGLPAYGENESEKLKMNSIITEILGENKFSCGN
                     DVEVALAGSLACQPGICIILGTGAIASGITNDLKTARTSGWGYICGDEGSGYWIAKKG
                     VEIFGKQSDHRLERTALYDIFKNELKLENNFDLIFLIKDEYKQDRTKVANLAMLVYKA
                     ALKGDKYALDIYRQAAKECLLMVNGLTNQLEFTGVLNISYSGSVFNSKEFILDPLRNY
                     LQYNYTAFNLTAPILPPIKGAALNALKLHKGEIPQGVVHNLAKEPKEILV"
     gene            complement(3073..3627)
                     /locus_tag="K8M77_002252"
     CDS             complement(3073..3627)
                     /locus_tag="K8M77_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBI7086712.1"
                     /translation="MKIKDIKKLTDCKFLNLYKLDIENKVGNSKEYFIASRRTEKDLS
                     CVVNKHHKADGVMIIPITENDEFVLLKQFRPAINDYIYEFPAGLIDNGEDVIKAATRE
                     LFEETGLLASESEYLIKPSYTSVGMSDESVAVVKMKVYGNISTENLEENEEIEVIKVP
                     RKEAKNFVKENNVSIKTALVLSFM"
     gene            complement(3793..>4324)
                     /locus_tag="K8M77_002253"
     CDS             complement(3793..>4324)
                     /locus_tag="K8M77_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086713.1"
                     /translation="LFVYLTYLIIIKNKENNKNKLKTNKGWKITPYINLRISSFESRI
                     DLEKMRLMELIKNSIKLIDEKVAFVDCNSEGFYIKSKNELIYIEFLVGEQNIEKNKEN
                     VSLKNNEMVKLELYNTIRRTIRRYIWFGEENFINPLKSEFNIRFSSGNIIMKISKEDK
                     IIFNKDEENHTLKNES"
BASE COUNT         1553 a          597 c          437 g         1737 t
ORIGIN      
        1 ttattttttt aaaatattat tgtaacttct atgggtttct actctttcac caaagacact
       61 ttttatagtc tgaataaatt ctttattaat acaatattct tcattatata tttttatttt
      121 tttaggtaca ttagttatta aaccactaat aataatatct tctgtattta ttattcctac
      181 ttcagcagca tctgtaattt catttaaaaa atccttaaag atatcaagtc ccttagaatc
      241 tttaacctca taagatccat ttttttgaac tattatattt acttcttcta atttacaatc
      301 ttgtatatct acaaaatact ttaatagctt tatgaattca ttatattctt tttcaatcat
      361 ataatcttct actactttat ctataataca ttctatatcc tttaaaaaat ttttcattct
      421 aaaagttata aaaccttcta tgtttatata atcattttct aatatgaatt cttttatatt
      481 ctctataata tcatttatct tatttaaaca ataaaaatct ttatctgaac aaatcttttg
      541 atctcctttt aatattttat ttatagctaa atcaatatct aatatttcat catgttttaa
      601 aaagaaataa ttttcattta tataatgaag catttctttt tctctataat gttctactat
      661 aacattataa ataacattac ttatatataa cattatagta gctctcatat tttcatcaaa
      721 ttcactattt ccatagtata ttttaacgaa gtgagttcca tatgagatac tttctgacac
      781 accaattata atacctttac tctttaaaac attttttata ttctgcatgt cttttattaa
      841 atttttttcc tcctcataac ataatgtcat taaaagcatt tttctcactc ctttctttac
      901 ttttagtatg tattaattca catgtaatat tcaaaaatat aaattcatta atcgacaatt
      961 tctttaattt tttagtaaaa tgaaattata aaattttgag tgaggagatt attatgagaa
     1021 cttgttttgt tgattataga atttcagaat tagaagaaaa aaaactaaag gatttaaatt
     1081 ttaatataat taaggtacca aaacatccta aactatatga tgctatagat ggtcatccag
     1141 atatacaaat aaatatactt aatgaaaata ctcttttatt agctaaagat tcctctgaag
     1201 aattatttaa ttctattcca aaggacttaa atattataaa aagctcttat aacttacaag
     1261 ataaatatcc tggaaatgta attttaaatg ctgtaaactt aaaaaatgat tttatacata
     1321 atctaaaatt tactgatcct aagctaattg aatcagtaaa tggaaaaaac ttaataaaca
     1381 taaagcaagg atattctaag tgttctattg ctgtagtttc tgaaaaagcc ttaataactt
     1441 ctgataaagg aatttataaa gccctaaaac ctcatggatt tgatatatta ttaattccta
     1501 gtggtgatgt ttctcttcct ggtttagatt atggatttat aggtggaacc tgtggattaa
     1561 tttcaaaaaa tcaaatggct ttctttggta acttagaaaa tcatccctat ggaaatcata
     1621 ttaaaaactt tttattaaaa tatgatgttg aaccaattta tctttctaat ggtaaattaa
     1681 tagatagagg tagtatatta actatttaac aatcactata ttttactcaa tatagtaata
     1741 aatgttgata aaatagcata taaatattat tttttatatt tatcaccttt attgttacaa
     1801 cttaaatata tatttttcag ataataagtg tataaattta atatttactg aattattatt
     1861 taatttaatt catacttttc cattcccatt gattatacat ttaataatat agtaaaataa
     1921 aaccaagtgg taattataac tatgtattaa aagggggaaa agtatgtttt atttaggtat
     1981 tgatgcaggt ggaacaaaaa cgtcatttat gcttataaac catttaggag aggttttatc
     2041 aacttatact agtggtacat gtcatataca tcaagtaggt tttgatggtt ttagaaaagg
     2101 tattcaagaa ggtattgata agatttgtaa tgaaattaat atatgcagag atgattttag
     2161 ttactcattt ttaggtttac ctgcttatgg tgaaaatgaa agtgaaaaat taaaaatgaa
     2221 ctctataata actgaaatct taggagaaaa taaattttct tgtggaaacg atgtagaagt
     2281 tgccttagct ggctccttag cttgtcaacc tggaatatgt ataattctag gaacaggggc
     2341 aattgcctct ggtataacta atgatttaaa aacagcaagg actagtggat ggggatatat
     2401 atgtggtgat gaaggttctg gatattggat tgctaaaaaa ggtgttgaaa tctttggaaa
     2461 acaatctgat catagattag aaagaacagc actttatgat atatttaaaa atgaattaaa
     2521 actagaaaac aatttcgatt taatattcct tataaaggat gaatataaac aagatagaac
     2581 taaagtggct aatcttgcaa tgttagttta caaagctgct cttaaaggag ataaatatgc
     2641 tttagatata tatagacaag ctgctaaaga atgtcttcta atggtaaatg gattaactaa
     2701 tcaacttgag tttacaggag tattaaatat ctcttactct ggtagtgtat ttaactcaaa
     2761 agaatttatc ttagacccac taagaaatta tcttcaatat aattatactg cttttaatct
     2821 aacagcacct atacttccac caattaaagg cgctgcatta aatgccttaa aacttcacaa
     2881 aggagaaatt ccacaaggtg tagttcataa cttagctaaa gaaccaaaag aaattttagt
     2941 ataataatat attttaaaag gagtattatg aaaataagta ataaagttta taaatagtat
     3001 gaagaaaaac tcttaacata tttataaatt ttatcttgaa atttcataaa gctcctttaa
     3061 tttatttata atctacataa agcttaaaac caatgcagtt tttattgata cattattttc
     3121 tttaacaaag tttttagcct cttttctagg cactttaata acttctattt cttcattttc
     3181 ttctaaattt tctgttgaaa tatttccata aaccttcatt ttaaccacag ctacagattc
     3241 atcagacata cctactgaag tatagctagg cttgattaaa tattcacttt cactagctaa
     3301 aagtccagtt tcttcaaaaa gctctcttgt agcagctttt attacatctt ctccattgtc
     3361 tataagtcca gctgggaatt catatatata atcatttata gcaggtctaa attgctttaa
     3421 caaaacaaac tcatcatttt ctgttattgg aattatcata acaccatctg ccttatgatg
     3481 tttattaaca acgcagctta aatctttttc tgttcttcta cttgctataa aatattcttt
     3541 agaatttcca accttattct caatatctaa tttatataag tttaaaaact tgcaatcagt
     3601 taattttttt atatccttta ttttcacctt aatactcctc tcatattttc acttttaatt
     3661 aagctttaaa ttaatacaat ttaaaatata tctatatttt taactattct aatttaattt
     3721 tacacataaa aaggataaat ataaagtttc ctttacacct atcctaaaaa tctaattaat
     3781 ttctctttaa acttaagatt cattttttaa agtatgattt tcctcatctt tattaaagat
     3841 tattttatct tccttactaa ttttcataat tatatttcca cttgaaaatc ttatattaaa
     3901 ttcacttttt agtggattaa taaaattctc ttccccaaac catatatacc tcctaatagt
     3961 ccttcttatt gtattataca attctaactt taccatctca ttatttttta aagaaacatt
     4021 ttctttattt ttttctatat tttgttctcc tactaaaaat tctatataaa ttaattcatt
     4081 tttacttttt atataaaagc cttcactatt acaatctaca aaggcaacct tttcatcaat
     4141 caattttata gaatttttta ttaattccat caacctcatt ttctctagat ctattctact
     4201 ttcaaaggaa cttattctta gatttatata tggagttatc ttccatcctt tattggtctt
     4261 taatttattt ttattatttt ctttattttt tataataatt aaataagtta aatatacaaa
     4321 taaa
//
LOCUS       DAEVGI010000170         4311 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEVGI010000170 DAEVGI010000000
VERSION     DAEVGI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.171"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..180
                     /locus_tag="K8M77_002254"
     CDS             <1..180
                     /locus_tag="K8M77_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBI7086714.1"
                     /translation="ANLQARVNAEHGACQGKKDLATLAKQLNLDAIHDTVHEMCKDEA
                     RHGKAFAGLLNRYFK"
     gene            313..1053
                     /locus_tag="K8M77_002255"
     CDS             313..1053
                     /locus_tag="K8M77_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase"
                     /protein_id="HBI7086715.1"
                     /translation="MGKAYEKVYEVTYGETDGRKDCRITSMMNFFSDCCLSQEEKNSM
                     NYADNSSETTWVFFDYEIIVNRYPRYREKVKVKTYVESIRKFYSNRVFEAYDMDGALV
                     ARADVLAFLINKKTRRPARISDEEYEIHGLSKESSKLLRKKLKFEKFDKEDLEMKFHI
                     RYLDIDLNMHVSNIKYVEWILETVPVDIVLNYKMKKIKIKFEKEITYGHNVIIKSKII
                     KGEDEVKVLHKVENEEGESITLAETYWY"
     gene            1076..1834
                     /locus_tag="K8M77_002256"
     CDS             1076..1834
                     /locus_tag="K8M77_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase"
                     /protein_id="HBI7086716.1"
                     /translation="MSENKFMKKYDVLYYDSDVNKNIKMVPLMKIFGDVSAIHEEELA
                     YEGIKYLKDHQLSWIIYSYSIDIKKSIPYKSSINVETYLEGIKKFYACRVYKVYNEKN
                     ELVAEGKIIFLLIDLEKRRAVRIPKEYCELINMSDTGEVELKSTKVEKLIREDLESNI
                     SVRRSDIDFNKHVNNTKYLEWTMEATPECILDEYSLISAKIKYEKEVRLGDDVNIVCQ
                     WDEIEEGYKCLYKIVNNRLGEVSASIETIWKKEF"
     gene            complement(1956..3200)
                     /locus_tag="K8M77_002257"
     CDS             complement(1956..3200)
                     /locus_tag="K8M77_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI7086717.1"
                     /translation="MIYHDVVIIGGGASGITAAITAKDFGLDVAIVEGTDRIGKKILT
                     TGNGRCNITNSLISFPFQNFHSTNDGFFIKSLNKFSVEDTKAFFLNLGLPIIELEKGK
                     NYPQSLQASSVVDILKMALEEREIPVYNSYKVKSIHKSKGKFTLSTGNEDSPVIKCKK
                     LIMACGGKSAPKTGSDGSGYTLAKSLGHSIIEPNPAIVQLKLSHNKLKALSGIKFNGY
                     AEVLCYGKSVRKEFGEILFTDYGISGPPILQISREASLGCFKGKEVKILVDMMPDKSL
                     KELEDFLEGHFAVFSCREVINSLIGVVNKKMIPILLREAGIDNLHKPCYELTWKEKKA
                     LINLMKSWEFKCIGTNGFQAAQVTTGGINTKEVDAETLQSKLVDNLYFCGELLDVDGD
                     CGGFNLQWAWSSGFTAALSCTL"
     gene            3396..3965
                     /locus_tag="K8M77_002258"
     CDS             3396..3965
                     /locus_tag="K8M77_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086718.1"
                     /translation="MKFRITNENIEGYNTELKIRRMNYDQVVVNYKNNSGIKTFKIND
                     GELVSEGEVDDIIKKYTDLLKIKINRGTSALFYKGIIDSIEESIEEVKSLKVLNDFTK
                     STSKRGIWDKEILIYLNESYPIKIEASGRNFREDSYKFNIKVLEEAEFIEMCHFNIEK
                     LKNQIGWRERQLNVYKKIVKKIEKESNFE"
     gene            4164..4238
                     /locus_tag="K8M77_002259"
     tRNA            4164..4238
                     /locus_tag="K8M77_002259"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4197..4199,aa:Glu,seq:ttc)
BASE COUNT         1673 a          522 c          730 g         1386 t
ORIGIN      
        1 gctaatttac aagcaagagt aaatgcagag catggagctt gccaaggtaa aaaagattta
       61 gctactttag ctaaacaatt aaacttagat gcaatccacg acacagttca cgaaatgtgt
      121 aaagacgaag ctagacacgg aaaagctttc gctggattat taaacagata ctttaaataa
      181 gaatccatca caaattgttt gtattgatat atttataaag aaaacaccac taaaacacct
      241 agtttagtgg tgtttttcta gtttgaaagg tattttttga tttgatatac ttccaaatga
      301 ggagtgatta aaatggggaa agcttatgaa aaagtttatg aagttactta tggagaaaca
      361 gatggaagaa aagactgtag aataacttca atgatgaact ttttttcaga ctgttgctta
      421 agtcaagaag aaaagaattc aatgaactat gcagataata gcagtgaaac tacttgggtg
      481 ttttttgatt atgaaataat agttaataga tatccaagat atagagagaa agttaaggta
      541 aaaacttatg ttgaatctat aagaaagttt tattctaata gagtgtttga agcttatgat
      601 atggatgggg ccttagtagc tagagctgat gtattagcct tcttaataaa caaaaaaact
      661 agaagaccag ctagaataag cgacgaagaa tatgagattc atggattaag taaggagtct
      721 tcaaaattat taagaaaaaa attaaaattt gagaaatttg ataaagaaga tttagagatg
      781 aaatttcaca taagatattt agatatagat ctaaatatgc atgtaagtaa tattaagtat
      841 gtagagtgga ttttagaaac agtaccagtt gatatcgttc taaattacaa aatgaaaaaa
      901 ataaaaataa aatttgaaaa agaaattaca tatggtcata atgtaattat aaagtcaaaa
      961 ataattaagg gtgaagatga agtaaaggtt cttcataaag tagaaaatga agagggagaa
     1021 agcattactt tagcagaaac ttattggtat taaaattttt ctaggaggat taattatgag
     1081 cgaaaataaa tttatgaaaa aatatgatgt actatattat gattcagatg ttaataaaaa
     1141 cataaaaatg gttccactta tgaaaatctt tggtgatgta tctgcaatcc atgaggaaga
     1201 gttagcttat gaaggaatta agtatttaaa agatcatcaa ttaagttgga ttatttatag
     1261 ttactcaata gatataaaaa agtcaatacc atataaaagt tcaataaatg ttgaaactta
     1321 tctagaagga ataaagaaat tttacgcttg tagagtatat aaagtttata atgaaaaaaa
     1381 tgaacttgtg gctgaaggaa agattatttt cttattaata gatttagaga agcgtagagc
     1441 tgttagaatt cctaaagagt attgtgagtt aataaacatg agtgatactg gagaggtaga
     1501 gttaaaatca actaaagttg aaaaattaat tagagaagat ttagaatcaa atatttctgt
     1561 aagaagaagt gatatagatt ttaataagca tgttaataac actaaatact tagaatggac
     1621 tatggaagct acaccagagt gtattttaga tgaatatagc cttatatcag ctaaaataaa
     1681 gtatgagaag gaagttagac ttggggatga tgtaaatatt gtttgccaat gggatgagat
     1741 tgaagaaggt tataagtgcc tttacaagat tgtaaacaat agattaggtg aagtttcagc
     1801 ttcaatagaa acaatttgga aaaaagagtt ttaaaagtaa taaaaccctc ggtatggcta
     1861 ggagtttgta gcctagttat accaagggtt ttgtattaag taaaaataaa ttttacttaa
     1921 attaaaagtt tatattatct atagaaaata tattactaaa gggtacaaga aagtgcagca
     1981 gtaaatccag aactccaagc ccattgaagg ttaaatccac cacaatctcc atctacatct
     2041 aataattcac cacaaaaata aagattatct actaactttg actgaagagt ctcagcatca
     2101 acttcctttg tatttattcc accagtagtt acctgagctg cttggaatcc atttgttcca
     2161 atgcatttaa attcccaaga tttcataagg tttattaagg cttttttctc tttccaagta
     2221 agttcgtaac atggtttatg taaattatct atacctgctt ctcttaataa tatagggatc
     2281 attttcttgt taacaacacc tataagtgag tttataactt ctctgcaaga aaaaacagca
     2341 aaatgtcctt ctaagaaatc ttctaattcc tttaatgatt tatcaggcat catatcaact
     2401 aagattttaa cctctttacc tttaaagcat cctaaggaag cttctctaga tatttgtaat
     2461 attggagggc cagatattcc gtagtctgta aaaagaatct ctccaaattc ttttctaaca
     2521 ctttttccat aacataaaac ttcagcataa ccattaaact ttattcctga taaagcttta
     2581 agtttattgt gagatagttt taattgtact atagctggat taggttcaat aatagagtga
     2641 cctaaacttt tagcaagagt atatccagag ccatcagaac ctgttttagg agcagatttt
     2701 cctccacaag ccattattaa ctttttacac ttaatcacag gtgaatcctc atttcctgtg
     2761 gataaagtaa attttccttt agatttgtgg atagatttta ctttgtaaga attataaaca
     2821 ggaatttcac gttcttctaa ggccattttt aaaatatcta caacagaaga tgcctgtaaa
     2881 gattgaggat aatttttccc tttttcaagt tctattattg gtaaccctag attaagaaaa
     2941 aaggcttttg tatcttctac agaaaattta tttaaagatt taataaaaaa tccatcattt
     3001 gtactatgaa agttttgaaa tggaaaactt attaatgaat ttgttatatt acatctaccg
     3061 tttcctgtag taagaatttt tttgcctatt ctgtctgttc cttcaactat ggcaacatct
     3121 aatccaaaat cctttgctgt aattgcggct gttataccag aagctccgcc tcctataata
     3181 actacatcat ggtatatcat aagtttaatt tctcccttct aaatttgtat atattaataa
     3241 tcaaaataat tttctaaaat tcttatatgt ttaatcataa catttctaag tttgaattgt
     3301 atcacatttg aatggtaata gatacttaaa gagaagaaat tataaagtta tattattttc
     3361 tttaaggagc aatactaaat agtgaggtga atattatgaa atttagaatt accaatgaaa
     3421 atatagaggg gtataataca gagttaaaga ttagaagaat gaattatgat caagtagtgg
     3481 taaattataa aaataattct ggaataaaaa cttttaaaat caatgatgga gaattagttt
     3541 cagaaggaga agttgatgat ataattaaaa aatacactga cttattaaaa ataaaaataa
     3601 atagaggaac atcagcttta ttttataaag gtataataga ttcaattgaa gaatctattg
     3661 aggaagtaaa atccttaaaa gttttaaatg attttactaa atcaacaagt aaaagaggaa
     3721 tttgggataa agagatactt atatacttaa acgaaagcta tccaataaaa attgaagcaa
     3781 gtggaagaaa ttttagagaa gatagttata agttcaatat taaggttcta gaagaagcag
     3841 aattcattga aatgtgccat tttaatatag aaaaattaaa aaatcaaata ggttggagag
     3901 aaaggcagtt aaatgtctat aaaaaaatag ttaaaaaaat agaaaaagag agtaattttg
     3961 agtaaaatgg tgaattgtaa ttataatttg attaaatagc tataaatagc ctaaaataac
     4021 taaagaaagt gttgacataa ctttagtttt tgggtattat attccttgtc aggtcgccaa
     4081 gtgcgaaaga cgaaaaacaa aataaacaaa tttagtttaa agctaaaact ggtcaaaaat
     4141 gatgtggttt taagagttaa tgtggcccct tggtcaagcg gttaagacac caccctttca
     4201 cggtggtaac aggggttcga ttcccctagg ggtcaccata ttacggtcgc atagctcagc
     4261 tgggagagca cctgccttac aagcaggggg tcacaggttc gatccctgtt g
//
LOCUS       DAEVGI010000171         4308 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEVGI010000171 DAEVGI010000000
VERSION     DAEVGI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.172"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(51..1349)
                     /gene="uraA"
                     /locus_tag="K8M77_002260"
     CDS             complement(51..1349)
                     /gene="uraA"
                     /locus_tag="K8M77_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBI7086719.1"
                     /translation="MAKSVQNKEVELLEVIGVEDKLPLKKAIPLSVQHLFAMFGSSVL
                     VPILLNIDPATVLFFNGIGTLLYAFITKRKIPAYLGSSFAFISPVLLLMGEGYSFTEV
                     QSGFVVSGLLFATIAIIVGFVGTGWIRKLFPPASMGAIVTIIGLELAPTAADMAGFPV
                     GLSNSKVLNPTWVLVSMITIITVILASVLLRGFLKVIPILIGIVTGYLVSCFLGLVDF
                     TQVKEASIFVLPQIHMAKPSLNAILTILPATFVVVAEHVGHLVVTSSIVGKDLSKDPG
                     LNRSLLGDGLSTMISGFFGSVPTTTYGENIGVMALTKVYSSYVICGAGVLSVILGFSG
                     SLSALIRSIPTPVIGGVSLLLFGTIATSGLRTFIEEKVDYSKSKNLILTSVTMIVGLS
                     NIAVRFGAIEIKGMGLATIVAMILSISFMIFDKLGLMNEK"
     gene            1537..3120
                     /locus_tag="K8M77_002261"
     CDS             1537..3120
                     /locus_tag="K8M77_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI7086720.1"
                     /translation="MITVNNVSLRFGGRKLFEDVNLKFTPGNCYGVIGANGAGKSTFL
                     KILAGEIEPNTGEVSIPAKTRVSVLKQDHYQYDDCEVLKTVIMGNPRLFEIMEEKDAL
                     YAKPDFSDEDGIKAAELEAEFADLDGWEAESEASSLLQGLGIGTELHYSHVRDLKGDE
                     KVKVLLAQALFGKPDVLILDEPTNHLDIKAITWLENFLGNFEGTVIVVSHDRHFLNMV
                     CTQICDVDFGKIKLYVGNYDFWYESSQLALQMAKDQNKKKEEKIKELQNFIARFSANA
                     SKSKQATSRKKLLDKITLDDIQPSSRKYPFVGFKPEREVGNDILEVNGISKTVDGVKL
                     LDNVSFRVNKDDKIAFVGNERSITTLFKIINGEMEPDSGDYKWGVTITNAYFPKDNTE
                     FFENCELNLVDWLRQFSDEKSESYLRGFLGRMLFSGEEALKQASVLSGGEKVRCMLSK
                     LMLSSANVLTLDQPTNHLDLESITAVNNGLADYKSVLLFACHDHQFVETIANRIIELT
                     PEGIKFDKVCTYDEYLETK"
     gene            complement(3171..4130)
                     /locus_tag="K8M77_002262"
     CDS             complement(3171..4130)
                     /locus_tag="K8M77_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBI7086721.1"
                     /translation="MDKEIFRKIKKKNLEKKYIQIKIVGAFTAIVVLVFLGIRIALNN
                     KIELAKENIEEGEIKELGMEEATVELPKYKSTDIVPGRNVTFYGKNYAVNAKDVSKMI
                     EGSYDGNEKYVFLTFDDGPSPITEQVLDILKNENVKGTFFMLGSRLDSGQASKDILKR
                     AIEEGNAIANHSYSHNFKKLYPGNITDVNYFMDEFRKTNDVMRGILGVEFDTNVLRMP
                     GGYNSRVYYKDRNLEELDNNLESNKIISIDWNALNGDAEGKPYTLNEMIDYVKRTSRG
                     KNQVVLLMHDTFGKEKIVKILPEIIKYYKEEGYEFKTISDANV"
BASE COUNT         1497 a          706 c          574 g         1531 t
ORIGIN      
        1 tttatctttc atttaaatct cttaatagaa tctagctttt taattaatat ttatttttca
       61 ttcattaaac ctaatttatc aaatatcata aatgatatac ttaaaatcat agcaacaata
      121 gtagcaagtc ccattccttt aatttctatt gcaccaaatc ttactgctat gttacttaaa
      181 ccaactatca ttgtaactga tgttaatatt aagttttttg attttgaata atcaactttc
      241 tcttctatga aagttcttaa tcctgaagta gctatagtac caaataaaag caaactaact
      301 cctcctatta ctggtgttgg tatgcttctt attaaagctg ataagcttcc tgaaaatcct
      361 aagattacag aaagaactcc agctccacaa attacgtagg aactataaac ctttgttaat
      421 gccattactc cgatattttc accataagtt gtagtaggaa ctgatccaaa aaatccagat
      481 atcatagttg ataaaccatc tcctaaaagc gatctattaa gtccaggatc tttagataaa
      541 tcttttccaa ctatgctact tgttactact aaatggccaa catgctctgc aactactaca
      601 aaggttgcag gtaaaattgt taaaattgca tttaaacttg gtttagccat atgtatttgt
      661 ggcaatacaa agatgcttgc ctctttcact tgtgtaaaat ctactagtcc taaaaaacaa
      721 ctaactaaat atccagtaac tatacctatt aatataggta ttacctttaa aaatcctctt
      781 aataatactg aagctaaaat tacagttatt attgtaatca ttgaaactaa tacccatgtt
      841 ggatttaaaa ccttagaatt tgataagcct actgggaaac cagccatatc tgctgctgtt
      901 ggagctaact ctaatcctat tattgttact atagccccca tagaagctgg tggaaataat
      961 tttctaatcc atcctgttcc tacaaaaccc acaatgattg ctatggtagc aaacaataat
     1021 cctgatacta caaatccact ttgtacttca gtgaaggaat atccttctcc cattaaaagt
     1081 aatactggtg atataaatgc aaaacttgat cctaaatatg ctggaatttt tctttttgtt
     1141 atgaaagcat ataataaagt tcctattcca ttaaagaata agaccgttgc tggatcaata
     1201 tttaaaagaa ttggtactaa gactgatgat ccaaacattg caaataaatg ttggacactt
     1261 aaaggaattg ctttttttag aggtaacttg tcctctactc ctatgacttc tagtagttct
     1321 acctctttat tttgtacaga ttttgccata ttattatcct cccttttcta gactctcagg
     1381 tctaaattaa aagtttatat aaaatatagc ataccgttta taataattca atattttttt
     1441 gtcgcatttt atgttataat aagtattcat gtgcataaaa atagtttttt tgcattaaca
     1501 aattattaaa atttaaagaa gaaaggacag aacttaatga ttacagtaaa taatgtaagc
     1561 ttaagatttg gtggaagaaa actttttgaa gatgttaact taaagtttac tcctggaaat
     1621 tgctatggag ttattggcgc aaatggtgct ggtaaatcta catttttaaa aatattagct
     1681 ggtgaaatag agcctaatac aggagaagtt tcaattcctg ctaaaactag agtttctgtt
     1741 ttaaaacagg atcattatca atatgatgat tgtgaagttt taaaaacagt tataatggga
     1801 aatccaagat tatttgaaat aatggaagaa aaagatgctt tatacgcaaa gcctgatttt
     1861 tctgatgaag atggaataaa agctgctgaa cttgaagcag aatttgcaga tttagatgga
     1921 tgggaagcag aatcagaagc ttcttcatta cttcaaggct taggaatagg tactgagctt
     1981 cactacagcc atgttagaga ccttaaaggt gatgagaagg ttaaagttct tttagctcaa
     2041 gccttatttg gtaagccaga tgtacttatc cttgacgagc ctacaaacca cttagacata
     2101 aaagctatca cttggcttga aaacttctta ggaaactttg aaggtacagt tatagttgta
     2161 tcccatgata gacatttctt aaacatggtt tgtacacaaa tatgtgatgt tgactttgga
     2221 aaaatcaaat tatacgttgg taactatgat ttctggtatg aatcaagtca acttgctctt
     2281 caaatggcta aagaccaaaa caagaaaaaa gaagaaaaaa ttaaagaact tcaaaacttc
     2341 attgctagat tcagtgctaa tgcttctaaa tctaagcaag ctacttctcg taagaagtta
     2401 ttagataaga ttactttaga tgatattcaa ccttcaagca gaaaatatcc tttcgttgga
     2461 tttaagcctg aaagagaagt tggaaacgac attctagagg ttaatggaat atcaaaaact
     2521 gttgatggag ttaaacttct agacaatgta agtttcagag ttaacaaaga tgataagatt
     2581 gcctttgttg gaaatgaaag atcaataact actttattta aaataataaa tggagaaatg
     2641 gaaccagatt caggagatta taagtggggt gtaactataa ctaatgctta cttccctaaa
     2701 gataatactg aattctttga gaattgcgaa ttaaacttag ttgattggct tagacaattc
     2761 tctgatgaaa aatctgaatc ttacttaaga ggattcttag gaagaatgtt attctcaggt
     2821 gaagaagctc ttaaacaagc ttctgtatta tcaggaggag aaaaggttag atgtatgctt
     2881 tctaaattaa tgcttagctc agctaacgtt ttaactttag accaaccaac taaccactta
     2941 gacttagaat caataactgc agtaaacaat ggtttagcag attataagag cgtattatta
     3001 tttgcttgtc acgaccacca atttgttgaa actatagcaa acagaatcat agaattaact
     3061 ccagaaggaa ttaaatttga taaagtttgc acatatgatg aatatttaga aactaagtaa
     3121 taaaaagagc gtgtgttgaa atttttattt caatacacgc tctcttcatt ttatacatta
     3181 gcatcactta tggttttaaa ctcataccct tcttctttat aatattttat tatttcaggt
     3241 aatattttta ctatcttttc ttttccaaag gtatcatgca taagtaaaac cacttgattt
     3301 tttcctcttg atgttctttt tacataatca atcatttcat ttaaagtata aggctttcct
     3361 tctgcatcac catttagagc attccaatct atacttatta ttttattgct ctctaaatta
     3421 ttatctaact cttctaaatt tctatcctta taatatactc tactattata gcctcctggc
     3481 attcttaaaa catttgtatc aaactcaaca ccaagaattc ccctcattac atcattagtt
     3541 tttctaaatt catccatgaa ataattaaca tctgttatat ttcctggata aagcttttta
     3601 aaattatgac tataactatg attggctata gcatttcctt cttctatggc ccttttcaaa
     3661 atatctttag atgcttgtcc agagtctagt ctagaaccta gcataaaaaa agtccccttt
     3721 acattttcat tttttaatat atctaaaacc tgttctgtaa ttggacttgg tccatcgtca
     3781 aaagtaagaa aaacatactt ttcattccca tcataagagc cttctatcat tttacttaca
     3841 tcttttgcat taacagcata atttttgcca taaaaagtaa catttctacc tggaacaata
     3901 tctgttgatt tatattttgg aagctcaact gtagcttctt ccatacctaa ttccttaatt
     3961 tctccttctt ctatgttttc cttagctaat tcaattttat tatttaaagc aattcttatc
     4021 cctaaaaaaa ctaaaacaac tatagcagta aatgctccaa caatttttat ttgtatatat
     4081 ttcttctcta aatttttctt ttttattttt ctgaaaatct ctttatccat aatacctcca
     4141 aaacacaact taacttattt tattatataa aatttcaact tttgaaatta taatctttaa
     4201 tttcaaaatg atcttattta atattaagtt tttaaataac atatctctct atacaaacag
     4261 tatactcaaa agaaaaattt atattatttt ttaaaaaaat aactctat
//
LOCUS       DAEVGI010000172         4289 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEVGI010000172 DAEVGI010000000
VERSION     DAEVGI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.173"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            100..615
                     /locus_tag="K8M77_002263"
     CDS             100..615
                     /locus_tag="K8M77_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086722.1"
                     /translation="MDIADINILVKYLSNLPMKEYKKNFTFLGEGIARKVFALNKNLV
                     VKVAKGEDGLHQNFVESYIFKNAPYNLKKYLCPVLLYNKRILIMPRAEVFPDIRRKTF
                     VDLKRLREESSIEEDIYTLGEKYFLFKEDLYVHSSWGIVNDNYYLIDYGCTSCEGDLY
                     YDFLMRTNGIL"
     gene            698..1933
                     /locus_tag="K8M77_002264"
     CDS             698..1933
                     /locus_tag="K8M77_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI7086723.1"
                     /translation="MNKFESMLENYAKLATHIGVNVQEGQTLVISSPVECAEFTRMLV
                     KSAYEKGAKDVVVQWNDEICGKIKYEHSPLEVFENFPDWMKESRLSYAKEGACFLSIS
                     ASDPELLKNIDPAKIAAFRKSSSTALKEFSEMLMSNKNSWSIVSIPTKAWAKKVFSDL
                     PEKEAVDKLWNEIFKIVRVDTENPVEAWNKHKETLKYHMDYLNEKNLKSLHFENSLGT
                     DLTIELPENHLWAGGAEYTQDGVEFIANMPTEEVFSMPSKFGVNGTVFSSKPLNYGGN
                     LIDNFSVTFKDGKVVDFSAKKGYDTLKHLLDTDEGAKYLGEVALVPYNSPISNSGIIF
                     FNTLYDENASCHLAFGKAYSLCIKNGENMTNEELEKAGANDSLTHVDFMIGTKDLKIT
                     GLTHDNVEIPVFKDGNWAF"
     gene            complement(2126..3175)
                     /locus_tag="K8M77_002265"
     CDS             complement(2126..3175)
                     /locus_tag="K8M77_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086724.1"
                     /translation="MDKLKQFLKWGLLSVILQCLVLFVFDKFYFKLNSDVNVKAIDIA
                     SQHTKENKDVKLPDNITDKKISYDGEYIAYLNNNDVLKVLNTLKNDENNVDVENEQIS
                     YYTWLRDRNRLIVITNTKYNNKKNTVNLYSIDVDTNKTKKIELGNINSSYKVNEITVS
                     TKTGVIYIEFKNEDTKESIVKRIDNNDDITDVKLITDSPGNIEVMPRADRLVYDSEKE
                     SGIYLTQPNKKLGINPIYKVRLLGVDETGNIYFGEIKDNKVFKIVYGNVEKSIDTWNK
                     IPLEKSLELNKIYIGKDGEIYVLEEEQKIVKDIKNGKEYKYEGDFLQMYSNGFATIIN
                     GNKLHRVYFDSYKNA"
     gene            complement(3192..4202)
                     /locus_tag="K8M77_002266"
     CDS             complement(3192..4202)
                     /locus_tag="K8M77_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBI7086725.1"
                     /translation="MGRDSRKKRRSKKKWKTIAIFIAFEFIFTAVTAPFILLYGPFKN
                     AKRTYVGAAMTSFNHQWMATTFLSDEKINDILNSNIEDTNTNHKNTNIKTNVNLPTKH
                     DNSIELYSFENFKYSGYYIVVKDPTRVKIGVSKYLGEEGQTTSEIAREYNAVAAVNGG
                     AFTDKSSTAQWTGNGGTPAGIVISEGKLVYKDVPDDKKIELVGITKEGKMIAGMYSFN
                     NLKELNVKEAVSFGPVLVKEGEPTPMKGDGGWGVAPRTAMGQRADGSIVMLVIDGRSL
                     TSGGATLKELQEVLLNTCNVVTAMNLDGGKSTTMYLNGKVINNPASNVGERSIPSAII
                     VK"
BASE COUNT         1473 a          644 c          525 g         1647 t
ORIGIN      
        1 gtaatttatg tttattatct caataatata ttacttcaat caacttttat taaagttcct
       61 tcatagaata tagaataaaa agtttcgagg ttttttatta tggatatagc tgatattaat
      121 atacttgtga aatatctaag caatcttcca atgaaagaat ataaaaagaa ttttaccttt
      181 ttaggagaag gaatagcaag aaaagttttt gctttaaata aaaatttagt tgttaaagtt
      241 gctaaaggtg aagatgggct tcatcaaaat tttgttgaat cctatatttt taaaaatgct
      301 ccttataatc taaaaaaata tttatgtcct gtacttcttt ataataaaag gattttaata
      361 atgcctagag ctgaggtttt cccagacata agaagaaaaa cttttgttga tttaaaaaga
      421 ttgcgtgaag aaagttctat agaagaagat atttataccc ttggagagaa atattttcta
      481 tttaaagaag atttatatgt tcacagttct tggggaattg taaatgataa ctattattta
      541 atagattatg gctgcacctc ttgtgaagga gacttatatt acgactttct catgagaaca
      601 aatggaattt tataaaataa tctttattaa ttaattgttt tataattttt actgcattat
      661 aataatggta agaatttgta aagaaaggat tgatattatg aacaaatttg aaagcatgtt
      721 agaaaattat gcaaaattag ctactcatat aggagtaaat gttcaagagg gtcaaaccct
      781 tgttatctct tctcctgtag aatgtgcaga atttacaaga atgcttgtta aatctgctta
      841 tgaaaaagga gcaaaggatg ttgttgttca atggaatgat gaaatatgcg gcaaaatcaa
      901 atatgaacac tctccattag aagtttttga aaactttcca gattggatga aagaatcaag
      961 attaagttat gctaaagaag gagcttgctt cttaagtatt tctgcctctg atcctgaact
     1021 tctaaaaaat atagaccctg caaaaatagc agcctttaga aaatcatcaa gtacagcttt
     1081 aaaagaattt agtgaaatgt taatgagcaa taaaaattca tggtcaatag tttctattcc
     1141 aactaaagct tgggctaaaa aagttttctc tgatttacct gagaaagaag cagtagataa
     1201 attatggaat gaaatcttta aaatagttag agttgataca gagaaccctg ttgaagcttg
     1261 gaataaacat aaagaaactt taaaatacca tatggattat ttaaatgaaa agaatttaaa
     1321 atcacttcat tttgaaaatt cacttggaac tgatttaact atagaattac cagaaaatca
     1381 tctttgggct ggtggagctg aatacactca agatggagtt gaattcatag ctaatatgcc
     1441 tactgaggaa gtattctcta tgccttctaa atttggagtt aatggaacag tatttagttc
     1501 taaaccttta aactacggtg gaaatttaat agataatttc tcagttactt ttaaagatgg
     1561 aaaagttgtt gatttctcag ctaaaaaagg atacgacact ttaaaacatc ttctagatac
     1621 tgatgaaggt gctaaatact taggagaagt agctcttgtt ccttataatt ctcctatatc
     1681 aaactcagga ataattttct tcaacactct atatgatgaa aatgcttctt gtcatttagc
     1741 ttttggtaaa gcatattctc tatgcataaa aaatggtgaa aatatgacta atgaagagct
     1801 tgaaaaagct ggagctaatg attcattaac tcatgtagat tttatgatag gaactaaaga
     1861 tttaaaaatt acaggtttaa ctcatgataa tgttgaaatt ccagtattta aagatggtaa
     1921 ctgggcattt taatatctaa aaacatattt taaatatatg attaaattaa accttcaaaa
     1981 taaacacatg ttttaaatat aaatttaaaa acatatgcta atttaaagta aaaggcaaag
     2041 aataacttat ttaaatgtaa attattcttt gcctttttta tataaattat tttaaactca
     2101 ttttagattc tatataatat ttccactaag catttttata tgaatcaaaa tatactctat
     2161 gaagtttatt tccatttata attgttgcaa atccattaga atacatttgt aagaagtcac
     2221 cttcatattt gtattctttt ccatttttaa tatctttaac aatcttttgt tcttcttcta
     2281 aaacgtatat ttctccatct ttccctatgt agatcttatt tagctctaag cttttttcta
     2341 gaggtatttt attccaagta tcaattgact tttctacatt tccataaact attttaaata
     2401 ctttattatc ttttatttct ccaaagtata tatttccagt ttcatcaaca cctaaaagtc
     2461 taactttata tattggatta attcctaatt tcttatttgg ctgtgtaaga tatattcctg
     2521 attctttttc actgtcatat actaatctat ctgcccttgg cattacttca atatttcctg
     2581 gtgaatcagt tattaatttt acatcagtta tatcatcatt attatctatt ctcttaacta
     2641 tagactcttt tgtatcttca tttttaaatt caatgtatat aactcctgtt ttagttgaaa
     2701 ctgttatctc attaacttta taagaagaat ttatattacc aagctcaatt tttttagttt
     2761 tatttgtatc tacatcaata gaatataaat taactgtatt ctttttatta ttatatttag
     2821 tattagtaat tacaataagc ctatttctat ctctaagcca agtataataa gaaatttgtt
     2881 cattttcaac atcaacatta ttttcatcat ttttaagagt attaagaact ttaagtacat
     2941 cattattatt taaatatgct atatattctc catcataaga tatcttttta tcagttatat
     3001 tatctggtaa ttttacatcc ttattctctt ttgtatgttg gctagctata tctatagctt
     3061 ttacatttac atcagaattt aacttaaaat aaaatttatc aaaaacaaat aaaactaagc
     3121 attgtaaaat tacagacaat agtccccact ttaaaaattg ctttaattta tccataaaat
     3181 tctcctcttt attattttac tattatagct gaaggaatag atctctcccc tacatttgat
     3241 gctggattat ttattacttt tccatttaag tacatagtag ttgatttacc accatcaagg
     3301 ttcatagcag taactacatt acaagtattt aataatactt cctgtaattc ctttaaagta
     3361 gctcctccac ttgttaaact tctaccatca ataactaaca ttactattga tccatcagct
     3421 ctttgtccca tagcagttct tggagcaact ccccatccac catcaccttt cataggtgta
     3481 ggttctcctt ctttaactaa aacaggacca aaacttacag cttccttaac atttaattct
     3541 ttaagattat taaatgaata cattcctgca atcatttttc cttcttttgt tataccaact
     3601 aactcaattt tcttgtcatc tggtacatct ttataaacta atttcccctc tgatataact
     3661 attccagcag gagttcctcc attaccagtc cattgagccg tactagattt atctgtaaaa
     3721 gctcctccat ttacagcagc aacagcattg tattctctag ctatctcaga agtagtttgt
     3781 ccttcttctc ctaggtattt agaaactcct atttttactc tagtaggatc ttttacaact
     3841 atataatatc cactatattt aaagttttca aaagagtata attctatact attatcatgt
     3901 ttagttggta aattaacatt tgtctttata ttggtatttt tatgatttgt atttgtatcc
     3961 tcaatattag aatttaatat atcattaatt ttttcatctg ataaaaatgt agttgccatc
     4021 cactgatgat taaaactagt cattgctgca cctacataag tccttttagc atttttaaat
     4081 ggtccatata aaagtataaa aggtgctgta acagcagtaa atatgaattc aaaagcaatg
     4141 aatattgcta tagtcttcca tttcttttta cttctcctct tctttctact atctctcccc
     4201 atttttcctc cttaatttaa aatgattaaa tttcctttgt aatctaaacc aactaaccct
     4261 tacattctat tgtttttaaa atattactt
//
LOCUS       DAEVGI010000173         4233 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEVGI010000173 DAEVGI010000000
VERSION     DAEVGI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.174"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..550
                     /locus_tag="K8M77_002267"
     CDS             <1..550
                     /locus_tag="K8M77_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086726.1"
                     /translation="LEREYDPYDGERYELGRRFAEYENEIEQMKLRDCKEKEEKTYEV
                     NFDCPIGEVLMGALEGCKKIPEFAEDIKRCAWYKVDVRNFDDMCNMSNYNKYTMMYYP
                     MINYYPYISKEGHFFFGIKCDKYGNIKYILYAIPGTKDRKDQPYGGRTGFVTWDRYRE
                     RENGYWIMFYDFENSSVVIPMK"
     gene            699..1271
                     /locus_tag="K8M77_002268"
     CDS             699..1271
                     /locus_tag="K8M77_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086727.1"
                     /translation="MNKKQFGITLILVFLMIILVTGENMFTGNIKKVALTNNNIEGLS
                     TYTALDGLLTYKLPDSWDVEEKKYPGNYIIYDNSFISDTMGLWGYVQILNSNDDLEKM
                     VEKEKELISNNDISDYLVSNESLDDELVKKVVFKEKNQKGVTYVNTVYYKKLGENKII
                     KVLFSASESKQKEDYSIIYKAIIDSIEHED"
     gene            1374..2144
                     /locus_tag="K8M77_002269"
     CDS             1374..2144
                     /locus_tag="K8M77_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBI7086728.1"
                     /translation="MRIVLGEIFGLKIYSYGFMIGLGIICATLLFLKRGTQRGYNEDK
                     LFNATILTVILGILGGKILYIITEWKTVIQDPSLIFRDFGNGFVIYGAIIGGALGIAL
                     CSLKNKWNVLELADLVVPGLALAQGFGRIGCLLAGCCYGAETTSSIGIIFPADSLAPA
                     GIPLYPTQIFSSIFDFALGLFLLWYGNKNKEKGKTMSMYMIIYSIGRFFVEFLRNDPR
                     GSVGSLSTSQFISIFILIGGILLYNINKLKGRKETSEK"
     gene            2134..2973
                     /locus_tag="K8M77_002270"
     CDS             2134..2973
                     /locus_tag="K8M77_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086729.1"
                     /translation="MKNKKLILVILCLVLGLGIVGCSAKDISSKFVGSNNNDFEYIKE
                     NKVDKIVIQSTRDSGFRFLVKEKSVIDNIYSMLSKAEVVSKKTDLPPDYIFEIHVGDE
                     EKKFYYVTGVKNNGEGNFYDGDHFYRISKRLDNYLMQNMQAIRKPRAFDEIYYNSILE
                     VMKKDKDELNKDNAKVGIDILGDRDCTKYMLSSDIEDFEKDVKKVVPNASIMDHNRDD
                     FDIIVTVRNYGYTSTMFRTVITIENRLTHSEKKFYVDGNYKNSWDINVYDSWKDVQKE
                     WDR"
     gene            2994..3848
                     /locus_tag="K8M77_002271"
     CDS             2994..3848
                     /locus_tag="K8M77_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBI7086730.1"
                     /translation="MGSCASCANKDKCSSASKDGGCSSSIPAKLGTNYGNIKNVIGVI
                     SGKGGVGKSTVTGILATQLAKKGYKVGVLDADITGPSMPRFFGISEKRADIVAMDSEG
                     KQVKFIPVKTELGIKVISMNLLMEVEDDPVIWRGPMVTGVLNQMFKDTDWEELDYLLI
                     DMPPGTSDITLTVMQTFPIKELVIVSTPQDMVSMIVKKLVTMAHKMNVCVRGVVENMA
                     YIECECGKKMRVFSKKSSEEHAEYLGLPLIGELPINLDLTEALENGKAEEYVAENPLY
                     SLIFEGLY"
     gene            3991..4179
                     /locus_tag="K8M77_002272"
     CDS             3991..4179
                     /locus_tag="K8M77_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBI7086731.1"
                     /translation="MKANVDKDTCIGCGLCPTIAPEVFDMDDDGKAHTIVEEVPENSK
                     EAAKEAEGSCPVAAISVE"
BASE COUNT         1679 a          396 c          803 g         1355 t
ORIGIN      
        1 tttagaaaga gaatatgatc cttatgatgg agaacgttat gagctaggta gaagatttgc
       61 agagtacgaa aatgaaatag agcaaatgaa acttagagat tgtaaggaga aagaagaaaa
      121 aacttatgaa gttaactttg attgtccgat aggtgaagtt ttaatgggag ctttagaagg
      181 ttgtaagaag attcctgaat ttgcagagga tataaaaaga tgtgcatggt ataaggttga
      241 tgttagaaac ttcgatgata tgtgtaatat gtcaaattat aataaataca caatgatgta
      301 ttatcctatg attaactact acccttatat aagcaaagaa gggcatttct tttttggtat
      361 aaagtgtgat aaatatggaa atataaaata tattttatat gctattccag ggactaagga
      421 tagaaaagat caaccatacg gtggtagaac tggttttgtt acatgggata gatatagaga
      481 aagagaaaat ggatattgga taatgtttta tgattttgaa aactcatctg tagttatccc
      541 tatgaaataa tcaagagaaa aactgtttat atagatttta tttctatata gacagttttt
      601 tatttatgta atattaatac tctatttagt aatttttact taattattga atatatttta
      661 aatatagact ttcctaaaag atgaaaagag gtgatttttt gaataaaaaa caatttggta
      721 taaccctaat attagttttt cttatgataa tattggtaac tggtgaaaat atgtttactg
      781 gaaatataaa aaaagttgca ttaacaaata ataatattga agggctaagt acatataccg
      841 ccctagatgg tcttttaacc tataagttac ctgatagttg ggacgttgaa gaaaaaaaat
      901 acccaggaaa ttatattatt tatgataata gttttataag tgacactatg ggcttatggg
      961 gatatgtgca gattttaaat tcaaatgatg atttagaaaa gatggttgaa aaagaaaaag
     1021 agcttattag taataatgat ataagtgact atttagtatc taatgaaagt cttgatgatg
     1081 aattagtaaa gaaggtagtt tttaaagaaa aaaatcaaaa gggagtaacc tatgttaata
     1141 ctgtttatta taaaaagtta ggagaaaata aaataataaa ggtattattt agtgcaagtg
     1201 aaagtaagca aaaagaagat tattcaataa tatataaagc cataattgat agtatagagc
     1261 atgaggatta gaaaatttga tttgctttaa tagaagtatt ataattaaat ttaaaagaga
     1321 agcataataa tatgcttctc ttttaaattt acaaataaat attggaggag tttatgagga
     1381 ttgttttagg ggaaatcttt ggacttaaga tttatagcta tggatttatg ataggattag
     1441 gtataatctg tgcaacatta ttattcttaa agcgaggaac tcaaagaggc tataacgaag
     1501 ataagctatt taatgctact atcttaactg ttattttagg aattttagga ggaaaaatac
     1561 tctacataat aactgagtgg aaaacagtta ttcaagatcc aagtttaata tttagagatt
     1621 ttggaaatgg ctttgtaatt tatggagcca taataggagg agccttagga atcgctctat
     1681 gctctttaaa gaataagtgg aatgtattag agcttgcaga tttagtagta ccaggtttag
     1741 ccttagccca aggatttgga agaataggat gtttacttgc aggatgttgt tatggggctg
     1801 aaactacaag ttctatagga attatatttc cagctgattc attagctcca gccgggatac
     1861 cattatatcc aacacaaatc ttttcgtcaa tttttgattt tgccttaggt ttatttttac
     1921 tatggtatgg aaataaaaac aaagaaaaag gaaaaactat gagcatgtat atgataatat
     1981 acagtatagg tagatttttt gtagagtttt taagaaatga tcctagaggt agcgtaggat
     2041 cattatcaac atctcaattt atatcaatat ttatattaat agggggtata ttactatata
     2101 atataaataa attaaaaggg agaaaagaaa caagtgaaaa ataagaaatt aattctagta
     2161 atactttgct tagttttagg attaggcatt gttggatgtt cagctaaaga tataagctca
     2221 aaatttgttg ggagtaataa taatgacttt gaatatataa aagaaaataa agttgataaa
     2281 atagttattc aaagtacaag agattctggt tttaggtttt tagtaaaaga aaagtcagta
     2341 atagacaata tatacagtat gttatctaaa gctgaagttg ttagcaaaaa gacagattta
     2401 cctccagatt atatttttga aatccatgtt ggtgatgaag aaaagaaatt ttattatgta
     2461 acaggtgtaa aaaataatgg agaaggtaat ttctatgatg gagatcactt ttatagaata
     2521 tctaagagat tagataacta tttaatgcaa aatatgcagg caataagaaa acctagagct
     2581 tttgatgaaa tatactataa ttcaatactt gaagtaatga aaaaagacaa agatgaacta
     2641 aataaagata atgccaaagt tggtattgat atacttggag atagagattg tacaaaatat
     2701 atgttatcat cagatattga agattttgaa aaagatgtaa aaaaagtagt tccaaatgct
     2761 tcaataatgg accataatag agatgacttt gatataatag ttactgtaag aaattatggt
     2821 tatacatcta ctatgtttag aactgtaata actattgaaa acagattaac tcattcagag
     2881 aaaaaattct atgtagatgg aaattataag aactcttggg atatcaatgt ttatgatagt
     2941 tggaaagatg ttcaaaagga atgggataga taacagaatg gaggaatttt cttatgggaa
     3001 gttgtgcaag ttgtgcaaat aaagacaaat gtagttcagc tagtaaagat ggaggatgta
     3061 gttcatcaat accagcaaaa ttaggaacta actatggaaa tattaaaaat gttataggtg
     3121 ttataagtgg taaaggtgga gttggtaaat caactgtcac tggaatatta gctactcaac
     3181 tagctaaaaa aggatataaa gttggagtat tagatgctga tataacagga ccatcaatgc
     3241 caagattctt tggaataagt gaaaaaagag cagatatagt tgctatggac agtgaaggta
     3301 agcaagttaa atttattcca gtaaaaactg agctaggaat aaaagttata tcaatgaact
     3361 tacttatgga agttgaagat gatcctgtaa tttggagagg accaatggtt acaggagtat
     3421 taaaccaaat gtttaaagat actgattggg aagagttaga ctacttatta atagatatgc
     3481 caccaggaac aagtgacatt acattaacag taatgcaaac attccctata aaggaattag
     3541 taatagtttc aacacctcaa gacatggtat caatgatagt taaaaaacta gttactatgg
     3601 ctcacaaaat gaatgtttgt gtaagaggag tagttgaaaa tatggcttac atagagtgtg
     3661 agtgtggcaa gaaaatgaga gtatttagca aaaaatcttc agaagagcat gctgaatatt
     3721 taggacttcc tctaatagga gaattaccaa taaacttaga cttaactgaa gctttagaaa
     3781 atggaaaagc tgaagaatat gtggctgaaa atccattata ttcattaatt tttgaaggat
     3841 tatattagta taaataaaag aatgaatcaa aattatatta ttttggttca ttctttttat
     3901 tttttaaatt aagtttattt ataaagtaat ataaattaaa atttagggaa taatgatatt
     3961 gttaatataa attttaagga ggcaataatt atgaaagcca atgtagataa agatacatgt
     4021 attggatgtg gattatgtcc aacaatagca ccagaagttt ttgatatgga tgatgatgga
     4081 aaagctcata caatagttga ggaagttcct gaaaatagta aagaagcagc aaaagaagct
     4141 gaaggtagtt gtccagtagc agctatttca gttgaataat aattaaaatc aaaaagggct
     4201 gtatcaaaat ataataaaac ttacaaacag tat
//
LOCUS       DAEVGI010000174         4131 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEVGI010000174 DAEVGI010000000
VERSION     DAEVGI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.176"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(91..747)
                     /locus_tag="K8M77_002273"
     CDS             complement(91..747)
                     /locus_tag="K8M77_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086732.1"
                     /translation="MARKSSKKYLKEANAQIILFVLIMIIMRIVVLNFMSEISGIEFD
                     ELSKARIYNGDITAILFLESLVNFFGNFILETYKGIILLAIINVFIASYILTKKDFTG
                     NSYFNIVIAYLFSAIITVFLLLTGIILFFSIMQCMVVINSLNIDVSSEILWLAIAVLI
                     GVVFIKYFRKSVVFSLLAFLIISVYASYYTDIGKKAINTDYTQYSLEEFANNLNLVEE
                     "
     gene            1054..2253
                     /locus_tag="K8M77_002274"
     CDS             1054..2253
                     /locus_tag="K8M77_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI7086733.1"
                     /translation="MNKLKIVSLFMSSFLMISAFSAPLITKANPEVNKLQEEKKQITE
                     EKEIKKGKLDEIKDSIDAKQAELNSAQAKVTEYEEKIKTLNNEISSTDSEISKVEKSI
                     EENTTEIEKAKKEQELKKEILGERLRNVYKSNLNDKFLYMILESKNFGELFSNIANIR
                     TLVKTDNKLIEEIESLKNELEVRQAELETSKSTLASKKVELLSKEKSLNEVKAEHENT
                     LNTYKSQLNELEELESEKNAELKELADREDEIEKEIQSYFTKTPSTASPTSNSSSGFI
                     RPVASSTITSSYGPRVHPVTGQYKVHTGVDFAASAGTPFIAAKDGVVTAAEYHPAYGN
                     MVIIDHGGGFSTLYAHASQLKVSVGQKVKQGQVVSLVGSTGYSTGPHAHFEIRINGQH
                     VNPMDYI"
     gene            2476..3339
                     /locus_tag="K8M77_002275"
     CDS             2476..3339
                     /locus_tag="K8M77_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HBI7086734.1"
                     /translation="MKNSKITAIVLSAIIGIGVGTGGTLVYENNQEAKQISKLEQTTK
                     ANAVEEIVKGNVLATLWQQNSGEVKALRYEAYNSGKRYVDELVKEPTAKPYAITLDID
                     ETIIDNSPHAGYEIKHNELYSKENFGEWVQMADAAAIDGAKDFTDYAKSKGFEVFYVS
                     NRSEEKELDATIKNMQKLGFVNSDKDHILLKTDTSSKDGRWNKIKDNYNLAIYCGDNL
                     GDFPDGYDNKSNEQRREIVDKEAQFFGTKYIVLPNPTYGDFENAVYGYDFKKSPEQKL
                     EDRLNSIKSFK"
     gene            complement(3551..3742)
                     /locus_tag="K8M77_002276"
     CDS             complement(3551..3742)
                     /locus_tag="K8M77_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086735.1"
                     /translation="MILKIKKGEYGKMFTNELKDLLAGLYQKYGCTQEVLQLSNIIDE
                     IIVKEQRERLKEYYKSRKK"
BASE COUNT         1699 a          516 c          507 g         1409 t
ORIGIN      
        1 ataaataaaa atatattaaa gtgattaaat gtaatttact aaataataaa tagaaaacac
       61 ttctttacta ttaaatataa tcatatagat ctattcttca actaagttta aattattagc
      121 aaactcctct agactatatt gagtatagtc tgtgttaatt gcttttttac ctatatcagt
      181 gtagtaagaa gcataaactg atattattaa aaatgcaagt aaagaaaaaa ctacactttt
      241 cctaaaatac tttataaaga ctactcctat taatacagct atggctaacc ataaaatttc
      301 tgaagacaca tctatattta atgaatttat aactaccata cattgcatta tgctaaaaaa
      361 taaaattata ccagttaata ataaaaatac tgttattatg gctgaaaata gatatgctat
      421 gactatatta aaataagaat tacctgtaaa gtctttttta gttaatatat aagaagcaat
      481 aaatacattt attattgcaa gtaaaattat tcctttatat gtttctaaaa taaagttacc
      541 aaaaaaatta acaagactct ctaaaaataa tattgctgta atatctccat tataaattct
      601 agccttagat aattcatcaa actctattcc tgatatttca ctcataaagt ttaaaactac
      661 tattctcatt attatcataa ttaagacaaa taatattatt tgagcatttg cttcctttaa
      721 atatttttta gatgattttc tagccataag aaattccccc tcttttataa ttcattttaa
      781 ttattgacaa gtagtatcaa tcttaatcac taaaatatac aaatccaact taatcactaa
      841 aatatacaaa tccaaaatat atttattaat caaactctaa actaatattt aaataaacac
      901 taataagaaa acataattta aatttttaat aatataaaaa taaattaact taaaatttgc
      961 atttttttaa tggatattac taagggtttt aaggtataat ttgttgaaat tactataaaa
     1021 ctctcgaaaa atatattaaa gataggtgat taaatgaaca aacttaaaat tgtatctttg
     1081 tttatgagta gctttttaat gattagtgct ttttcagccc cattaataac taaagcaaat
     1141 cccgaagtta ataaattaca agaggagaaa aaacaaatta cagaagaaaa agaaattaaa
     1201 aagggaaaac tcgatgaaat aaaagatagc attgatgcta aacaagctga gcttaattca
     1261 gctcaagcaa aagttactga atatgaagag aagataaaaa ctttaaataa tgaaatttct
     1321 tcaactgatt cagaaataag taaagtagaa aaatcaattg aagaaaatac tactgaaatt
     1381 gaaaaagcta aaaaagaaca agagttgaag aaagaaatat taggtgaaag acttagaaac
     1441 gtatataaat ctaacttaaa cgataagttt ttatatatga ttcttgagtc taaaaacttt
     1501 ggagaattat tctctaatat agcaaacata agaactcttg ttaaaacaga taataagctt
     1561 attgaggaga ttgaatcttt aaaaaatgaa cttgaagtta gacaagctga acttgaaact
     1621 tctaaatcta cattagctag taaaaaagtc gaacttcttt ctaaagaaaa aagtcttaat
     1681 gaagttaaag ctgaacatga aaatacatta aatacttata aatctcaatt aaacgaatta
     1741 gaggaattag aatctgaaaa gaatgctgaa cttaaagaat tagcagatag ggaagatgaa
     1801 attgaaaagg aaattcaatc atattttact aaaactccaa gcacagcatc tccaactagt
     1861 aattcatcaa gtggatttat aagaccagtt gcttcttcta ccataactag ttcatatggt
     1921 ccaagagttc accctgtaac tggtcagtat aaagttcata caggtgtaga ctttgcagct
     1981 tcagcaggaa ctccatttat agctgctaaa gatggggttg taactgctgc tgaatatcat
     2041 cctgcttacg gaaatatggt tataatagac cacggtggtg gattcagtac tctatatgct
     2101 cacgcatctc aattaaaagt ttctgtaggt caaaaagtaa aacaaggaca agtagtttct
     2161 ttagttggtt ctacaggata tagtacaggc cctcatgctc attttgaaat aagaattaat
     2221 ggacaacatg taaatccaat ggattatatt taatagaaaa gatagtcatt tgactatctt
     2281 tttttattta cataattatt aattaaattt ttataagaat aattttacat atcaattatt
     2341 gattattaaa agatatatat tattaattaa atacttgatt ttaatggaaa cataaaatat
     2401 aatgaaagcg ttaacacatt gataaccatt ttaatacttt gtacattttt atgcaacaaa
     2461 tgaaaggatg agattatgaa aaactcaaaa attacagcaa ttgtattatc agcaataata
     2521 ggtattgggg ttggaactgg aggtacttta gtttacgaaa acaatcaaga agcaaaacaa
     2581 atctctaagt tagaacaaac aacaaaggct aatgcagtgg aagagattgt taaaggaaat
     2641 gttttggcaa ctttatggca acaaaattct ggagaagtaa aggctttaag atatgaagca
     2701 tacaactctg gaaagagata tgttgatgaa ttggtgaaag aacctactgc taaaccctat
     2761 gcaatcacct tagatattga tgaaacaata atagataatt cacctcatgc agggtatgaa
     2821 ataaagcata atgaattata ttcaaaagaa aactttggtg agtgggtaca aatggctgat
     2881 gcagctgcaa tagatggagc taaagacttt actgattatg ctaaaagtaa aggatttgaa
     2941 gttttctacg tttcaaatag aagcgaagaa aaggaacttg atgcaactat aaaaaacatg
     3001 caaaaattag gttttgtaaa ttcagataag gatcatatac ttttaaaaac agatacaagt
     3061 agtaaagatg gtagatggaa taagataaaa gataattata atcttgcaat ttattgtgga
     3121 gataacttag gtgacttccc tgatggatat gataataaat ctaatgaaca aagaagagaa
     3181 atagtagata aagaagctca attctttgga acaaaataca tagttcttcc aaatccaact
     3241 tatggtgatt ttgaaaatgc cgtttatgga tatgatttca aaaaatctcc agaacaaaaa
     3301 cttgaagata gattaaatag tataaaatca tttaaataaa ctttaattaa tttaaagaag
     3361 tttaattata catattatga ataaaaaatc tatattacta actgtaatat agattttttt
     3421 attataatat atgttaacaa aatcaaagtc ttacggccct taaaattaat tgtaataatc
     3481 ataattcaat tgtttttcat aatttagagt taataatatt tatatgcttt ataacttttg
     3541 aaattagata ttattttttt cttgatttat aatattcttt tagtctttct ctttgctctt
     3601 taactataat ttcatcaatt atattgctaa gttgaagaac ctcttgagta catccatatt
     3661 tctgatataa acctgctaat aaatctttta actcatttgt aaacatctta ccatactctc
     3721 cctttttaat ctttaatatc atctcctatt aaaacatcat atttcttacc tataaaatct
     3781 ttaattactt agataattta ttaaaatcat ttatatttaa agagaaatca ttacttaaga
     3841 taataaatca tcctttaact atattctaaa attatttaac taattagtaa ataattttag
     3901 aattacaaaa ttcgaccatg aatatttatt aatttattat gagtttttat actatttatc
     3961 cttttttaac cataacttat taataaaata tgaatttgct cttctacctt tttaattaca
     4021 cttcatatct taccaataaa aaccatttat tgtaataaaa aaatttttta ttttaaaaaa
     4081 ctacttattt ttgtaaaaaa aatacacttt tagtttaaaa ttttaaaaca t
//
LOCUS       DAEVGI010000175         4035 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEVGI010000175 DAEVGI010000000
VERSION     DAEVGI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.177"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(20..1165)
                     /locus_tag="K8M77_002277"
     CDS             complement(20..1165)
                     /locus_tag="K8M77_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI7086736.1"
                     /translation="MYNLIDARGATWYRGTGIGTYTYNLLTNILKLDKDSSYDLLCSV
                     EKIPEFESENTKIIMSSRKHQRFFENYYIPSYGIKEDIDLLHLPQNGLGLSSEGNFAK
                     LVTIHDLIPYILPETVGKGYLKKFIQSMPEIIDNSTGIITVSEYSKSDIIRFFPHFPA
                     ENIFVTPLAANENYKPLDKEKCLFDVNKRFNFNGPFIVYIGGFSLRKNVKGLVDAFNS
                     IHKNIDENYKLLIVGGLRDQGLKLKAYTESLPIKDKIIFTGFIEDEYLPTLYNATTLF
                     VYPSLYEGFGLPPLEAMSCKTAVLTSNITSIPEVVPFKESLFNPNNPKELSLKLENLL
                     NDSKLRNNLENICFERSKEFTWEKTAKKTLDVYKKVIEISKNSSIGE"
     gene            1294..2301
                     /locus_tag="K8M77_002278"
     CDS             1294..2301
                     /locus_tag="K8M77_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HBI7086737.1"
                     /translation="MEHDYIKDKILLNYGIEVKEVIKVKNTYKIITSDEEYCLKVIKY
                     QYPHFYFIVSAQKHLMKNGFNSIPKILDTVYGKDYIKLDDKLAYLTPWVKCRECDYKN
                     RLDLSLAAKKLSELHNSSEGFVINRDLKPRIAWGKWYKIFETRGEEILDFKKRIYQKA
                     YMSDFDKLYLSIMDEELKRVERTLFHIENSGYFDYMKKEVKKLGFCHHDYANHNVLLL
                     ADNEINIIDFDYCILDSHLHDLSSLCIRTMKEGRWDLNLFKYIIDSYSKNKEVRNEDF
                     PIMASFIEFPQAYWQLGLQYYWEQQPWEEEHFLKKLGKYEKDREVRQNFVDELNNFTL
                     R"
     gene            2309..2992
                     /locus_tag="K8M77_002279"
     CDS             2309..2992
                     /locus_tag="K8M77_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086738.1"
                     /translation="MSEKLELFLRENNIEVLPRKEIEREKRELRFYDPVEYLNTLSEI
                     HKEFLKEENRIRFKFRNEVWKQVQIFKLWSRRVERIEESNGLINKALDASKKSLECIY
                     NISYKELIIRAMEREEVCIGRIYYEVKNGDKKIFIKNTENIKFNMVEEDYYNYLKKIR
                     GKYKDELKDLLLEVVEKESLDFKSYVYINSLIQYPYNSMRYLQNNYIKDLKIKSNELE
                     ELLVKDYLI"
     gene            3008..>4035
                     /locus_tag="K8M77_002280"
     CDS             3008..>4035
                     /locus_tag="K8M77_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003482172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HBI7086739.1"
                     /translation="MNKIRYKDEKYLCRYDLDIKLFEALGLDIFDLRPNRNVFLLDTK
                     QGKKILKMINYDDDRLNFIIHSTEYLRERYDGILKINKLPNGEWRFKWKGNDYILLDY
                     FEGTEFNIANPIELEIITEAVAKLHNAGRGIQEATSKEMNEKNSELFKLKDYFINSKK
                     DLEKLKEIVGSYKYKNEFDEIFIKEVDYHLSDVKVCIDLLEKSKYDDLCRDKEKITLC
                     HNDLAYHNILFNQNKVSFIDFDYCNINLRVIDLCNFIIKSIKRFGFSLEIYDSIIERY
                     DKLNNLSKEEKELMDIYLRFPHDFYTVSMQYYYKLKDWKYESFLNKLERKLEYTKEKE
                     ILLNHINK"
BASE COUNT         1619 a          377 c          663 g         1376 t
ORIGIN      
        1 taataattta tataaatttt tattctccta ttgatgagtt tttagagatt tctattacct
       61 tcttatatac atctaaggtt ttctttgctg ttttctccca agtaaattct ttgcttcttt
      121 caaaacatat attttctaaa ttatttctaa gttttgaatc atttaataga ttttctagtt
      181 ttagagataa ctccttaggg ttatttgggt taaataagct ttctttaaag ggaaccactt
      241 caggaataga agttatattt gaggttaaaa cggcagtctt acaactcata gcttctaaag
      301 gaggtagccc aaaaccttca tataaagaag gatagacaaa gagagtagtt gcgttataca
      361 aggttggtaa atattcatcc tctataaatc cagtaaaaat aatcttatct tttataggta
      421 aactttcagt ataagccttt aattttaatc cctggtctct taatccacct acaattaaaa
      481 gtttataatt ttcatcaata tttttatgaa tactgttaaa agcatcaact agtcccttaa
      541 cattttttct taaactaaag ccacctatat acacaataaa tggtccatta aaattaaatc
      601 tcttattaac atcaaatagg catttttctt tatctaaagg tttataattt tcattagctg
      661 ccagtggagt tacaaaaata ttttcagcag gaaagtgtgg aaaaaatctt ataatatcac
      721 ttttagagta ttctgataca gtgattattc ctgttgaatt atcaattatt tcaggcatac
      781 tttgaataaa tttttttaag taacctttac caacagtttc aggtaaaatg taaggaatta
      841 agtcatgaat agtaactaat tttgcaaagt ttccttcaga ggaaagtcca agtccatttt
      901 gaggaaggtg aagcaaatct atatcctctt ttattccata ggatggtata tagtaatttt
      961 caaaaaaacg ttgatgtttt ctagaagaca ttattatttt agtattttca ctttcaaact
     1021 caggtatttt ttctacagag cataataaat cataagatga atccttatct aattttaata
     1081 tgtttgttaa taggttatat gtgtatgttc ctatacctgt ccctctatac caagttgcac
     1141 ctcttgcatc tattaagtta tacattaaga ttccactcct tagaaaaata atcattataa
     1201 ttaataatat taataaataa tataaaatgt gcatgtaaca agaagaattt aagcaacata
     1261 tactaattaa aaagtgacag taggagtaag tttatggaac atgattatat aaaggataaa
     1321 atcttactta actatggtat agaagtcaaa gaagtaataa aagtgaaaaa cacatataaa
     1381 attataacca gtgatgagga gtattgtttg aaggtaataa agtatcaata tcctcatttt
     1441 tattttatag tttctgctca aaagcatctt atgaaaaatg gttttaattc aattcctaaa
     1501 atattagata ctgtttacgg aaaagattat ataaaattag atgataaatt agcttactta
     1561 actccttggg taaaatgtag agaatgcgat tataaaaata gattagattt aagcttagcc
     1621 gcgaaaaagc ttagtgaact acataatagt agtgaagggt ttgttataaa tagagatctt
     1681 aagcctagaa tagcatgggg taagtggtat aagatttttg agacaagggg agaagaaatt
     1741 cttgatttta aaaaaagaat atatcagaaa gcatatatgt cagactttga taagttatat
     1801 ctttctatta tggatgagga gttaaaaaga gttgagagaa cactttttca tatagaaaat
     1861 agtggatatt ttgactatat gaaaaaggaa gttaaaaaat taggcttttg tcatcatgat
     1921 tatgcaaatc ataatgtgct tttattagca gataatgaaa taaatataat agactttgat
     1981 tactgtattt tagattctca cttacatgat ttaagtagcc tatgcattag aacaatgaaa
     2041 gaagggagat gggatttaaa tttatttaaa tatataatag atagttattc aaagaataaa
     2101 gaagtaagaa atgaagattt ccctattatg gcttctttta ttgaattccc tcaagcttat
     2161 tggcaattag gacttcaata ttattgggaa caacagcctt gggaagaaga acatttttta
     2221 aagaaactag gtaaatacga aaaagataga gaggttagac agaactttgt tgatgaatta
     2281 aacaatttta ctttgaggtg atttaatttt gagtgaaaaa ttagaattgt ttttaaggga
     2341 aaataatata gaggtattac ctagaaaaga aatagaaaga gaaaaaagag aattaagatt
     2401 ttatgatcca gtagagtatt taaatacact aagtgagatt cacaaggaat ttttaaaaga
     2461 ggagaatagg ataagattta aatttagaaa tgaagtgtgg aaacaggttc aaatttttaa
     2521 actttggagt agaagagtag agagaataga ggaaagtaat ggattgatta ataaggcctt
     2581 agatgcctca aaaaaaagct tagaatgtat ttataatatt agttataaag agcttataat
     2641 aagagctatg gaaagggaag aagtatgtat tggtaggata tattatgaag ttaagaatgg
     2701 agataaaaag atttttataa aaaatacaga gaatataaaa tttaatatgg tggaagagga
     2761 ttattataac tacttaaaaa agataagagg aaaatataag gatgaattga aggatttact
     2821 tcttgaagta gtagagaaag aaagcttaga ttttaaaagt tatgtttata taaactcatt
     2881 gattcagtat ccttataatt caatgaggta tttgcaaaat aattatataa aggatttaaa
     2941 gattaaaagt aatgaattag aagaattatt agttaaagat tatcttatat agtaggggga
     3001 taggtttatg aataagatta gatataaaga tgagaaatat ttatgtagat atgatttaga
     3061 tattaaatta tttgaagctt taggactaga tatatttgat ttaagaccaa atagaaatgt
     3121 atttttatta gatacaaagc aaggtaagaa gattttaaag atgataaatt atgatgatga
     3181 cagattaaac ttcataatcc attcaacaga gtacttaagg gaaagatatg atggtatatt
     3241 gaaaataaat aagcttccaa atggagagtg gagattcaaa tggaaaggaa atgattatat
     3301 attattagat tactttgaag gaactgagtt taatatagct aatccaatag aattagaaat
     3361 cataacagag gcagtagcta aacttcataa tgcaggaaga ggaattcaag aggctacttc
     3421 aaaggaaatg aatgaaaaaa atagtgagct ttttaagtta aaagattatt ttataaatag
     3481 taaaaaagat ttagaaaaac taaaggaaat agtagggagc tataagtata aaaatgaatt
     3541 tgatgaaatt tttataaaag aagtagatta tcatttaagt gatgtaaaag tttgtataga
     3601 tttattagaa aagagtaaat atgatgattt gtgtagagat aaagaaaaaa taactttatg
     3661 tcataatgat cttgcctatc ataatatatt atttaatcaa aataaggtaa gctttataga
     3721 ctttgattat tgcaatataa atttaagggt tatagactta tgcaatttta taattaagag
     3781 tataaaaaga tttggattta gtttagaaat atatgattca attatagaaa ggtatgataa
     3841 gttaaataac ttatcaaagg aagaaaaaga gttgatggat atatatttaa gatttcctca
     3901 tgatttttat actgtttcaa tgcaatatta ttataaatta aaggattgga aatatgagtc
     3961 cttcttaaat aaattagaga gaaaattaga atacacaaag gaaaaggaaa ttttattaaa
     4021 tcatataaat aaata
//
LOCUS       DAEVGI010000176         4035 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEVGI010000176 DAEVGI010000000
VERSION     DAEVGI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.178"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            60..659
                     /locus_tag="K8M77_002281"
     CDS             60..659
                     /locus_tag="K8M77_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI7086740.1"
                     /translation="MRILDDITKFIFVCDEPKPCDLIFIPGSSKYEVSEKASLLYKQR
                     LAKYVLPSGKFSCKLEKFPNEKIINKKYAGKYESDWEFCKEVLFRNDVPLESILVENK
                     STNTSENAFFSRKVTDDLNIEVKSAILCCQAFHARRALITYSLAYPNTQFYVVPVETQ
                     GISKDNWYKSDYGINRVLGELRKCGSYFEEYIKDLNKII"
     gene            716..895
                     /locus_tag="K8M77_002282"
     CDS             716..895
                     /locus_tag="K8M77_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086741.1"
                     /translation="MFSKKEKRFENKFIENLDFGTIIVLVDKKTGVNYLLAQGPNGCS
                     LTPLLDSKGNVVVEK"
     gene            complement(1080..3595)
                     /locus_tag="K8M77_002283"
                     /pseudo
     CDS             complement(1080..3595)
                     /locus_tag="K8M77_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456264.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT         1263 a          702 c          454 g         1616 t
ORIGIN      
        1 ttttatatta aattacataa gttttaaaat taaggctata aatttagaga ggttatgaaa
       61 tgagaatact agatgatata actaaattta tttttgtttg tgatgaacca aagccttgtg
      121 acttaatctt tataccaggc tcatccaaat acgaggtaag cgaaaaggct agtttattat
      181 ataaacaaag attagcaaaa tatgtacttc cttcaggcaa gttttcttgt aaactagaaa
      241 aatttccaaa tgaaaaaata ataaataaaa agtatgctgg taaatatgaa tctgattggg
      301 aattctgtaa ggaagtttta tttagaaatg atgttccatt agaatccatt ttagttgaaa
      361 ataaatcaac taatacctct gaaaacgcct ttttctcaag gaaagttaca gatgatttaa
      421 atatagaagt taaaagtgct atattatgtt gccaagcctt tcatgctaga agagctttaa
      481 ttacttactc tttagcatat cctaatacac aattttatgt tgttccagta gaaactcaag
      541 gaatttctaa ggataactgg tataaatctg attacggcat aaatagagtt cttggtgagc
      601 taagaaaatg tggctcttat tttgaagaat atataaaaga tttaaataaa attatataaa
      661 attaacacct tatttttaag cttattaatt taaatatatt aaaaggagtt tttatatgtt
      721 ttctaagaaa gaaaaaagat ttgaaaataa gtttatagaa aacttagatt ttggaacaat
      781 aattgtctta gtggataaaa aaactggagt aaattatctt ttagctcaag gtccaaatgg
      841 ctgcagttta acccctttac tagattctaa aggaaatgta gttgtggaaa aataatgaga
      901 ttaattcttt aaacaatact atatgaaaac cactttaaat taatattcaa ttaaaatata
      961 gagtataaaa agagatatca aaatatatga tatctctttt tcattgattt tactatttaa
     1021 ttaagatatt tttattaatt ttcctttaaa gccaagaaat taatattaat ttataatgat
     1081 tatttatcct gcctatttct tctaatcact tttcttttgt aaaaaatcat tgctatacct
     1141 attccaatag ctataacacc aattattata ataactattc ttccgcccac tgagccagta
     1201 ttaggtaaga aacctgagtg tttaacatta actttacatg tagctttaaa gccaccatcc
     1261 ttagttgtta ctgttatttc agcttctcct gatgacttgg cctctatatt tccattctca
     1321 tcaactgata caccctttgg cttacttgat atccaagtaa cctccttgtt tcttgcatct
     1381 tttggactta tttcagcctt taaagtatat ttttcacctt ttttaaggtt taaatcttta
     1441 ttatttaatt tgacaccagt taccttaatt tattctggag gagtggcttc tttttcattt
     1501 accttaacat tgatttcctt agtttttgat gcaccttcac tatctgttac tttgtaaatt
     1561 actttataat ctccggcctt ttctgtatcc acattatttt ctataacctt aatatccttt
     1621 gttatatctc catcttcctt atccatagct gttacaccat ccattggatc aaacttatcc
     1681 ccaactgtta atgtaacatc tttagcattt atttctggag caattattta taactgaagg
     1741 tgatagacta tcgattttta agagctaaga tagcagttcc tccttcttct tttgaaggag
     1801 cttttacagt tacctttcca tctttgccta ttgaatattc atttccacta tataagtctt
     1861 taaccttctc tccttctttg aaatttgttt taaaacttat atctttatct tcatttttag
     1921 tgtttaatac aacaaataga ttttgtccat ttgtaatatt tttctcttca ttattttcac
     1981 ttaaattcat aggagtaatt tcttttccat tatagcttct taatactact gaatatcctt
     2041 cttcattgtt tcctgcaata gtttttctat ttccctttga aaatatctta gagtattctt
     2101 ttcttatatt aagaagtttt tcataatgat catgaacctt tttatactta ggatcattta
     2161 atctttcaaa atccatatca tatctatttt gatctccttg ttcaaagcca ttccatcctg
     2221 acattccaag ttcttctcca tagtatataa cagggatacc cttatctgtt atttgaagag
     2281 aagcagcaag catttgtttg cctaagtcat ctcccacagt ctttaggaat ccatcttcat
     2341 catgactact taagaattgt cccattaaat tggtattgct tattttactt gctctatcat
     2401 ctaaataagc tgaagttccc tcaatatttc catttacaaa atctcttgct tttcctttat
     2461 aaccaaagtc taataaagca tccatttgac cattttgaag ttgtcctcca tcgttattta
     2521 tatcagctcc aaaatattca ccaatagttt taaagcttgg gtttatttct gttaatttat
     2581 ttttaaatgc cttccaagtt gtattatcaa catgcttaac tgtatcaact ctaaaataat
     2641 caatagtatt tcctttttca gtctttgatc tttcaatcca tcctgtttgc cactctatta
     2701 atttttctct tacttctgga tcctctgttt taaaatctgg aagtcctgct gactcttgag
     2761 ttataaaatc atttcctgga ttttctctaa acattccttt gaacttagct atatcttcct
     2821 ctgttggaga gttattagct ccactattat ccttattttt cattccatag ccagcatgat
     2881 ttaaaacaac atcaaccatt atttttattc cactatcatg tgctgtatct attaatgttt
     2941 ttaagtcatc catagttcct aaatgtggat ctaatttttc aaaatcctta gcccagtaac
     3001 catgacatga gtattgagtt tttccttgag agaacatttg attaaaatca gtattctcaa
     3061 caataggagt tatccaaatt gtgtttattc caagttcttt taaatatgga atcttatctg
     3121 ttaatccttt taaatctcct ccatgataag tatatggatt cttcttatca tagttgtttc
     3181 catgaggatc atcattattc ttatctccat taaagaatct atctgtcact gtaaagtaaa
     3241 ttctagactc atcaaagcca aaatctaaag gtccaactcc aacctttgat ttaacagtta
     3301 aaattccttt tgctttatga ctttcaccat ttttatcaac tattgtaact ggtatttctt
     3361 tttctccagc tactacatta tcatttattc caatgctttg gcttaatgaa ttatccttta
     3421 ataaatttaa atccattaac accttattag ggccacctac atttgtaagg tccatataaa
     3481 cttcttttat attttctttt ttagcatcac ttccacttaa tttaagattt gtaatggcat
     3541 tttcatcata gttaacctga ttatttttga aagtaacctc tccatttatt tttaattgat
     3601 caggattatt tattgaatca tatacctttt tatttttata atcaaaccta aatattacct
     3661 ctgaatcttt tgataaattc aaagctatgt tttgaccatt attaccatag ctctcatccc
     3721 aagttccatt catggcaatt ttataattat aatttcctgc ctttaacttt aaagtgcctt
     3781 catataaacc atttttatac tttttcagca ctccattcat attttttgga tcccaatcag
     3841 aaccgttata attaacttgt aaaaaatctc ctacaactac agcaaaagct tcactctcat
     3901 tcttaactaa agtcttttca tttactttag tattattatt ttctacttca gccattactg
     3961 gcatatgagg taaaaagcta aacaagaatg ttaataaaac tagcatagaa atagacttaa
     4021 tctttttcct gctat
//
LOCUS       DAEVGI010000177         3974 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEVGI010000177 DAEVGI010000000
VERSION     DAEVGI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.179"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            103..1299
                     /locus_tag="K8M77_002284"
     CDS             103..1299
                     /locus_tag="K8M77_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBI7086742.1"
                     /translation="MNINLMNEAQEIKDLLVALRRDFHENPELGFEEWRTSGKIKEFL
                     TNEGIEYIETAKTGVCGIIKGTLKDESKKDRCIALRADIDGLPMDDKKTCSYSSKVKG
                     RMHACGHDAHTTILLGAAKLLSRHKDKFSGTVKLLFEPAEETTGGAPIMIEEGVLENP
                     RVEKIIGLHVEETLDAGEIMIKKGVVNAASNPFTIKIKGRGGHGAYPHMAVDPIVMAS
                     QVVLGLQTIVSREIKPVNPAVVTVGSINGGTAQNIIPDEVILKGVIRTMTLEDRAYAK
                     ERLREIATSICTAMRGECEIDIEESYPCLYNNSSVVDLITEAAKEIIGSQNVKEQEAP
                     KLGVESFAYFALERDSAFYFLGARNEERNIIYSAHNSRFDIDENLLPIGVSIQCKAAL
                     NYLTKE"
     gene            1359..2333
                     /locus_tag="K8M77_002285"
     CDS             1359..2333
                     /locus_tag="K8M77_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBI7086743.1"
                     /translation="MKIEIGTNEAGQRLDKFLRKYLKDVPLSAIFKALRKGDIRVNGK
                     KQKEKYSLLDGDVIEIRYLQSVVTKEKKKPNFQMVDSGSLQVVYEDENMLLVEKWPGV
                     LVHSDKKNGAPTLTDYVLSYLFRKGDYVPENELTFTPASCNRLDRNTSGIVFFGKNFE
                     GLKLLNEMIRERKVKKYYCALVKGKIQPGKYEAYISKDDELNKSTVYDTERPDTKKIK
                     MDVNVLQTNGAFSLLEIELITGRSHQLRAHLAHLGNPIVGDFKYGDNKMNSFFENKFG
                     LTYQFLYAYKVTFKEVPEKFDYLKNKTIAQGLPPMFKKIKKDVFKFRI"
     gene            2355..3971
                     /locus_tag="K8M77_002286"
     CDS             2355..3971
                     /locus_tag="K8M77_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBI7086744.1"
                     /translation="MKEQSITKGFAILSIASIVAKLLSLIYVPFLTRIVGNVGMGIYG
                     KTYDIFVFVYALTNVGLQTAISKYVSELDAVGNYKDALRAFKISRAFLLVVGTIFTIV
                     LMLSANFIASMSQTPQMVYPIIFLAPTIMITTVLVTYKGYFQGRSQATPIAIATVIEQ
                     VANVFLSLLCAYFLIKKSVSLGVAGGTIGSSAGALMAVLYLIYIYNIYGIEKEAKTKQ
                     EPGVRRITGKKIARTLFKYGLPITLSTGLQNLGNIIDMLTVSNRLVDAANFSQSDANA
                     LYGYFSTRYKILINVPMVFITSLGYMALPAISRAYALKDKKEIKSKINFSMRVTYLLS
                     IPSAFGLALLSENIYRYIFADSNGYKMMVIGAAMIPLTAIVLMQNVILQSVSQFYYVL
                     FTLALSIVVKFTLNMTLVANPDINIYGAVISGMVAFVISLVLNHFRMKKTLKMKISML
                     KYALKPLLASIYMGIGIILSKYVISLFIDFDKLSLILGIAILLVIIAIGSFMYLHAII
                     ILGGIKKEDIEEISPKLLRVMPRFLRKNIK"
BASE COUNT         1562 a          441 c          707 g         1264 t
ORIGIN      
        1 aaatataatt gaagaaaaaa agatagtaat atataatcaa aaatatataa aataaatagt
       61 atatataata tattatttat aaataaaaag taggtgttta aaatgaatat aaatttaatg
      121 aatgaagctc aagaaataaa agacttactt gtagctttaa gaagagattt tcatgaaaat
      181 cctgaattag gttttgaaga gtggagaact tcaggaaaaa taaaggaatt tttaactaat
      241 gaaggtattg aatatataga aactgctaaa acaggagtat gtggcataat aaagggcaca
      301 ctaaaggatg aatctaaaaa agatagatgt atagctttaa gagctgatat tgatggtctt
      361 cctatggatg ataaaaagac ttgttcatat tcatcaaagg ttaaaggaag aatgcatgct
      421 tgtggacatg atgcccacac aacaatatta ttaggtgcag ctaaattatt aagtagacat
      481 aaagataagt ttagtggtac tgttaagtta ctctttgaac cagcagagga gacaacaggc
      541 ggagctccta taatgataga agaaggagtt ttagaaaatc ctagagtaga aaaaataata
      601 ggtcttcatg ttgaagaaac tttagatgcc ggagaaataa tgataaaaaa aggagtagtt
      661 aatgcagcat ctaatccttt cacaataaaa ataaaaggaa gaggaggaca tggagcttat
      721 cctcacatgg ctgtagaccc tatagttatg gcttctcaag ttgttttagg attacaaaca
      781 atagtaagta gagagataaa gcctgtaaat ccagcagttg ttacagtagg aagtataaat
      841 ggaggaactg ctcagaatat aataccagat gaggttatat taaaaggtgt tataagaacg
      901 atgaccctag aagatagagc ttacgctaaa gaaagactaa gagaaatagc tacatctatt
      961 tgtacagcta tgagaggaga atgtgaaata gatatagaag aaagctatcc atgtctttat
     1021 aataatagct ccgttgtaga tttaataact gaagctgcaa aagaaattat tggttctcaa
     1081 aatgttaagg aacaagaagc accaaagctt ggagttgaaa gctttgcata ttttgcccta
     1141 gaaagagatt cagcttttta tttcttagga gctagaaatg aggaaagaaa tattatttat
     1201 tcagctcata atagtagatt cgatatagac gagaatttat taccaattgg agtttcaatt
     1261 caatgtaaag cagcattaaa ttatttgaca aaggagtaaa ttaatattaa tctatagaat
     1321 agtaattata aaatgattta aattaggagt gaagcaattt gaaaatagag ataggtacta
     1381 atgaagcggg acagagatta gataagttct taagaaaata tttaaaggat gtacctctaa
     1441 gtgcaatatt taaagcccta agaaagggtg atataagagt aaatggaaag aagcaaaaag
     1501 aaaagtattc tcttttagac ggagatgtta tagagataag atatcttcaa agtgtagtaa
     1561 ctaaggagaa gaaaaagcct aatttccaaa tggttgattc aggatcactt caagtggtat
     1621 atgaagatga aaatatgctt ttagttgaga aatggccagg agtattagtt cattcagata
     1681 aaaagaatgg agctccaacc ttaactgact atgtattatc atacttattc agaaaaggag
     1741 attatgttcc agaaaatgaa ttaactttta ctcctgcatc ttgtaacaga ttagatagaa
     1801 atacttcagg aatagtattt ttcggtaaga attttgaagg attaaaatta cttaacgaga
     1861 tgataagaga aagaaaagtt aagaagtatt actgtgcttt agtaaaagga aaaatacagc
     1921 ctggaaaata tgaagcatat atatcaaaag atgatgaatt aaataagtca acagtttatg
     1981 atactgaaag accagataca aagaaaataa aaatggatgt taatgttcta caaactaatg
     2041 gtgccttttc attattagaa atagaactta taactggaag aagtcatcag ttaagagctc
     2101 atttagctca tttaggaaat cctattgttg gagactttaa atatggtgat aataaaatga
     2161 attccttctt tgaaaataag tttggattaa catatcaatt cctttatgct tataaagtta
     2221 catttaaaga ggttcctgaa aagtttgatt acttaaaaaa taaaactata gctcagggat
     2281 tacctccaat gtttaaaaag ataaagaagg atgtatttaa atttaggatt tagtttttaa
     2341 gataggagat ttttatgaaa gagcagtcta taactaaagg ctttgcaata ctttctattg
     2401 caagcatagt tgctaaatta ttatctttaa tttatgtacc ttttcttact agaattgtag
     2461 gaaatgttgg tatgggtata tatggaaaaa cctatgatat atttgttttt gtgtatgctt
     2521 taacaaatgt aggtctacaa acagccatat caaaatatgt ttcagagtta gatgcagttg
     2581 gtaattacaa agatgcctta agagcattta agatttcaag agcattttta cttgtagttg
     2641 gtactatatt tactatagtt ttaatgttaa gtgctaattt tatagcaagt atgagtcaga
     2701 ctccacaaat ggtttatcca ataattttct tagcaccaac aattatgata acaacagtct
     2761 tagttactta taaaggatac ttccaaggaa gatctcaagc aacaccaata gctatagcaa
     2821 cagttattga acaggttgca aatgtatttt taagtttatt atgtgcatat ttccttataa
     2881 agaaaagtgt atctttaggg gttgcaggag gaactatagg atcttctgct ggggcattaa
     2941 tggcagtatt atatttaata tacatatata atatatatgg aattgaaaaa gaggctaaaa
     3001 caaaacaaga gcctggagtt agaagaatta caggtaaaaa aatagcaaga actttattta
     3061 aatatggatt accaataact cttagtactg gacttcaaaa cttaggaaat ataatagata
     3121 tgttaactgt aagtaacaga cttgttgatg cagctaactt ttctcaaagt gatgctaatg
     3181 cactttatgg atatttttca acaagatata agattttaat aaatgttcca atggtattta
     3241 taacttcttt aggatacatg gcattacctg caatatcaag agcatatgct ttaaaagata
     3301 aaaaagagat aaaaagtaaa attaatttct ctatgagagt tacatatctt ttatcaatac
     3361 catcagcctt tggattagct ttattaagtg aaaatatata tagatatatt tttgcagatt
     3421 caaatggata taagatgatg gtaataggtg ccgctatgat accattaacg gcaatagttc
     3481 ttatgcaaaa tgtaatactt caaagtgtaa gtcaatttta ctatgtatta tttaccttag
     3541 ctcttagtat tgtagtgaag tttacattaa atatgacttt agttgcaaat ccagatataa
     3601 atatttatgg agctgtaatt tctggaatgg tggctttcgt aatttcttta gtacttaatc
     3661 actttagaat gaaaaaaaca ttaaagatga aaatatcaat gttaaagtat gcattaaaac
     3721 ctttattagc atcaatatac atgggtatag gaataatact tagcaaatat gtaatatctt
     3781 tatttataga ttttgataag ttaagtttaa tattaggcat agctatatta ctagtaatta
     3841 tagctatagg ttcatttatg taccttcatg ctattataat tctaggagga attaaaaagg
     3901 aagatataga agaaatttca cctaaacttt taagagttat gccaagattt ttaagaaaaa
     3961 atataaaata attt
//
LOCUS       DAEVGI010000178         3964 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEVGI010000178 DAEVGI010000000
VERSION     DAEVGI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.180"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            76..633
                     /locus_tag="K8M77_002287"
     CDS             76..633
                     /locus_tag="K8M77_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086745.1"
                     /translation="MSNLDCFLSLLIGNFDNSEQFKDFENNGVKDFPFAEHVNTVCNN
                     KIKNLPENFKGKFLLEESYYTSNGKTHASPHLFLFTEETKGIKLTSYEIPEGYDKKSF
                     NYENMSLVDYSDLKISEKFTPAFYVFKENFWEGGSVSNFSPIMKFTLFERFSERQLEV
                     SETIEVNGKRTFGYDNPIIYKRINK"
     gene            1070..3628
                     /locus_tag="K8M77_002288"
     CDS             1070..3628
                     /locus_tag="K8M77_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBI7086746.1"
                     /translation="MQFNLKRKVLITTALTTTLLTGFCNLNAYGSVKNTSVNDFRNVL
                     NVQGNPQVNLNDSYSTNVSNPFSDMGAWHAYYLPEKGATNLYGGFAGPLIVGEEYPIN
                     LSDTISKITLTNSDTGEVYDLSKAKNIMFDFYPGKLVQTYELDDFNLKLELIFATNRS
                     ALIKTEIENKKNTDLNLNLEWSGNIFNKSPYTISVNENGEKNYDLGQTLEATQNGVQV
                     NFSNIRSTWSFFSTDQTKFNVEYDGNTSTAINGNTYVTKLDNVVNIKPENTFKTFSTQ
                     SYTFTTEEFNNEQVKINDLLENGDTYFNDNTNRWQNYLNKAFSNTEKDSDPHYKDAAV
                     KSIITLTTNWRSAAGAIKHDGIVPSLSYKWFVGMWAWDSWKQAVATANFDGELAKNNI
                     RALFDYQIKGDDSVRPYDKGTIIDAIFYNKDEQRGGEGGNWNERNSKPPLAAWAVWNV
                     YKETNDIEFLKEMYPKLVDYHNWWYTNRDHDKNGIAEYGGMVHPANNSEDEIILAAAW
                     ESGMDNATRFDKEGFGSDDAGVHVFENKDNNGNTVGYSINQESVDLNSYLYAEKGFLK
                     SMADILGKTEDSQKYEIEAKNVGEYIRNNMFDKETGFFYDLQINEDGSEKKLLVNRGK
                     GTEGWMPLWAKVATQDEGELVKENIMDPSKFNTYMPLPTASKDNPKFNPNKYWRGPVW
                     MDQALYGIESLQNYGFKDEAKTLTKKLFDNAEGLIGDGPIRENYNPETGQGLHTKNFS
                     WSASAYYLLYKNVLTSNNTTCQESLPILESDNITPPEDDFQKPAPPVIEEEKPETPPA
                     NDSSEQEKPVVKPEENNDNINSLPNTGNMFGNTSLLLGAFSMLGAGIYFLKKRN"
BASE COUNT         1572 a          441 c          636 g         1315 t
ORIGIN      
        1 gacagtaagg gaaaaaaata gtatacttaa ttaaattggt tacctaaaat tattaaattg
       61 tatggaggag aaaatatgag taatttagat tgttttttaa gcttattaat tggaaatttt
      121 gataactctg aacaattcaa ggattttgaa aataatggag ttaaggattt cccgtttgca
      181 gaacatgtta atacagtttg taataataag ataaaaaatt tgccggaaaa ttttaaaggg
      241 aaatttcttt tagaagaaag ctattatact tctaatggta aaacacatgc atcaccacat
      301 ctatttttgt ttacagaaga aacaaaagga ataaagctta cttcttatga aattccagaa
      361 ggttatgata aaaaatcttt taattatgaa aatatgagtt tagtagatta ttcagatctt
      421 aaaatatctg aaaaatttac tcctgctttt tatgtgttta aagaaaattt ctgggaaggt
      481 ggaagtgtta gcaacttctc tcctattatg aaatttactt tatttgaacg tttttcagaa
      541 agacaattag aggtatctga aactatagag gtcaatggaa aaagaacatt tggatatgat
      601 aaccctatta tttataaaag aataaacaaa taaaaaacta cttaatgtta ctatatctta
      661 ttatttaagg gattagccct atataataaa taattcattc tgtgagagta gctaaaattt
      721 aaactgtcca acatttggtg agagtttaat ttagctacta ttttttttat aatatactaa
      781 ctagtctata ccttcataat acattctgta gatattctgc catagcccat taatataaat
      841 tttattaata tttaacttat atacacattt aaatagaaag tattttttta taagttaagt
      901 atatttaata ttttgttaaa tattaatgta tcgattgaaa atgttatcat aagatgttac
      961 tattttattg aattcgacaa ccatattgtt acttttaagg gagtatatta acgaagtagc
     1021 ataaatttat aatgtttgtt tgatattact ttttaagggg gattttaaaa tgcaatttaa
     1081 tttaaaaaga aaggttttaa ttacaacagc attaacgaca accttattaa ctgggttttg
     1141 taatttaaat gcatatggaa gcgtaaaaaa tacttctgta aatgatttca ggaatgtttt
     1201 aaacgttcaa ggaaatccac aggtaaactt aaatgattcc tattcaacta atgtatctaa
     1261 tccattttcg gatatgggag catggcatgc atattattta cctgaaaaag gagctacaaa
     1321 tttatacggt ggatttgcag ggccgttaat tgtaggggaa gagtatccaa ttaacttatc
     1381 agatactata agcaaaataa ctttaactaa tagtgatact ggagaagtat atgatttatc
     1441 aaaagccaaa aatataatgt ttgattttta tccaggtaaa ttagttcaaa catatgaatt
     1501 agatgatttt aatttgaaat tagaacttat ttttgcaact aatagaagtg cattaattaa
     1561 aacagaaatt gaaaataaaa aaaatactga tttaaattta aatctagagt ggtcaggaaa
     1621 tatatttaat aaatcaccat atactataag tgttaatgaa aatggagaaa aaaattacga
     1681 tttaggtcag acattagaag caacccaaaa tggtgtacaa gttaattttt ctaacataag
     1741 atctacatgg tcattttttt ctacagatca aacaaagttt aatgttgagt atgatggaaa
     1801 tactagtaca gctataaatg gaaatacata tgtaactaag cttgataacg ttgtaaatat
     1861 taaacctgaa aatacattta aaactttttc tactcaaagt tatacattta caaccgaaga
     1921 gtttaataat gaacaagtta aaataaatga tttacttgaa aatggtgata catattttaa
     1981 tgataatact aatcgttggc aaaattattt aaataaagct ttttcaaata cagaaaaaga
     2041 tagtgatcca cattataaag atgctgctgt aaaatcaata attactttaa ctactaactg
     2101 gagaagcgca gcaggagcta ttaaacatga tggaatagta ccatctttat catataaatg
     2161 gtttgtagga atgtgggctt gggattcatg gaaacaagct gttgcaactg caaactttga
     2221 tggagaacta gctaaaaata atattagagc tctatttgac tatcagataa aaggtgatga
     2281 ttcagttaga ccatatgata aaggtacaat aatagatgca atattttata ataaggatga
     2341 gcaaagagga ggagaaggtg gaaactggaa tgaaagaaat tctaaaccac cactagcagc
     2401 ttgggcagta tggaatgttt ataaagaaac taatgatata gaatttttaa aagaaatgta
     2461 tcctaaatta gttgattacc ataattggtg gtatacaaat agggatcatg ataaaaatgg
     2521 aatagctgag tatggtggta tggtgcatcc tgctaataat agtgaagatg aaataatatt
     2581 agcagcagca tgggagagcg gtatggataa tgctactagg tttgataaag aaggatttgg
     2641 ttcagatgat gctggagttc atgtttttga aaacaaagat aataatggaa atacagtagg
     2701 atactcaata aaccaagaat cagttgattt aaactcttat ttatatgcag aaaaaggatt
     2761 cttaaaatca atggctgata ttttaggaaa aacagaagac tctcaaaaat atgaaataga
     2821 agctaaaaat gtaggagagt atattagaaa taatatgttt gataaagaaa ctggtttttt
     2881 ctatgattta caaataaatg aggatggaag tgaaaaaaaa ttattagtta atagaggaaa
     2941 aggaactgaa ggttggatgc cactttgggc taaagttgca actcaagatg aaggtgaatt
     3001 agttaaagaa aatattatgg atcctagtaa atttaatact tatatgcctt tacctacagc
     3061 atcaaaagat aatcctaaat ttaatccgaa taaatattgg cgtggaccag tttggatgga
     3121 tcaagcttta tatggtatag aatcacttca aaattatggt tttaaggatg aagctaaaac
     3181 tttaactaaa aaattatttg ataatgcaga aggattaata ggagatgggc ctattaggga
     3241 aaattataac cctgaaacag gacaaggtct acatactaaa aactttagct ggtcagcttc
     3301 agcatattat ttattatata aaaatgtatt aacttctaat aatacaactt gtcaagagag
     3361 tttaccaatt ttagaaagtg ataatataac tcctccagaa gatgattttc aaaagccagc
     3421 accaccagta atcgaggagg aaaaaccaga aacacctcct gcgaatgatt cttcagaaca
     3481 agaaaaacct gttgtaaagc cagaagaaaa caatgataat ataaattcat tacctaatac
     3541 aggaaatatg tttggtaata cttctttatt gcttggagct tttagtatgc ttggagcggg
     3601 aatatatttc ttaaaaaaaa gaaactaata cattaatata aataaatcat attattgatt
     3661 gataaaaaat ggtagtccat atataatcaa taaaagattt taaaatatta agttgttaaa
     3721 atctaagctt ttctatttat aaatttaaag tgcctataag aacatatagt ttttataggc
     3781 acttttatca taaaattaat tattattaat actaattaat ttttatagaa tttaagtgat
     3841 taagttaata atatttctgc tattctagct ccagcagaaa tacctaacat tgttaatgta
     3901 atagcaaatg aaacagtcat tatattcacc tcacttttta attaaatttt tatgattata
     3961 tttt
//
LOCUS       DAEVGI010000179         3928 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEVGI010000179 DAEVGI010000000
VERSION     DAEVGI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.181"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..974)
                     /locus_tag="K8M77_002289"
     CDS             complement(<1..974)
                     /locus_tag="K8M77_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HBI7086747.1"
                     /translation="MKFKIDNIENLSDSRQAMESKPNKLMFIFLYIIIALIVTFFIWS
                     WFSEKEIIVKVNGVVRPDNEIKAVSNIVQGEVESVKMKNGENVSKGEVLFKIKSTELE
                     DKKNQLDQQIEYINTDNENLEKLNKSINDNTNYFENNDKEKEYYYKFQSYEAGNKVSI
                     EEKNNISSSKDDLNNEKDDLETLSRSISENKNLNKEGSAYSSQYESYVSSKEMIQNKT
                     EQLENSKKALSEEIEVNNKEKNKLSDENSKAIIDDKNKQINNQISQIDLEIKNNNEQL
                     DKLKSDTLAQIKGSIDKINQSLSKLDSNISSLDESANIAKDKNKTTVLA"
     gene            complement(964..3180)
                     /locus_tag="K8M77_002290"
                     /pseudo
     CDS             complement(964..3180)
                     /locus_tag="K8M77_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            3324..3548
                     /locus_tag="K8M77_002291"
     CDS             3324..3548
                     /locus_tag="K8M77_002291"
                     /inference="COORDINATES: protein motif:HMM:NF016272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin family bacteriocin"
                     /protein_id="HBI7086748.1"
                     /translation="MEVLRMENLNLNQLENINGGYWKTIWAVGPGLYQRDTETGKYRW
                     IQTQDNLSYTTNVIANGWAGSAAGGYFSGR"
     gene            3585..3881
                     /locus_tag="K8M77_002292"
     CDS             3585..3881
                     /locus_tag="K8M77_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBI7086749.1"
                     /translation="MQNDLISRVYDLILDEKTPNDEKSKLIEFKNSVENGKDFEKEIM
                     HLAENLRKLSLVKFNNKEKLSSEVGKLYMDISSTGFIRNALGRGIVNLSFLAGK"
BASE COUNT         1299 a          624 c          426 g         1579 t
ORIGIN      
        1 gctaaaacag tagttttatt tttatctttt gctatattag ctgattcatc taaactacta
       61 atattactat ctaatttact taaactttga tttattttat ctattgaccc ttttatttga
      121 gctaaagtat cactttttag cttgtccaat tgctcattat tatttttaat ttctaaatca
      181 atctgactaa tttgattatt tatttgttta ttcttatcat ctataattgc tttactattt
      241 tcatcactta gcttattttt ctctttatta tttacttcaa tctcttcact taaagctttc
      301 ttactattct caagttgctc tgttttattt tgaatcattt cttttgaact aacataactc
      361 tcatattgtg aactatatgc tgaaccttct ttatttaagt ttttattctc agaaatagat
      421 ctactaagag tttctaagtc atctttttca ttattaagat catctttcga actagagata
      481 ttatttttct cctcaataga gaccttatta ccagcctcat aactttggaa tttataataa
      541 tattcttttt ccttatcatt attttcaaaa taatttgtat tatcatttat agacttatta
      601 agcttttcaa gattttcatt atcagtgttt atatattcaa tctgttgatc taactgattt
      661 ttcttatctt ctaattcagt agatttaatt ttaaataata cctctccttt agaaacattt
      721 tcaccgtttt tcatttttac tgattctact tctccttgaa caatgttaga aaccgccttt
      781 atttcattat ctggtctcac tacaccatta actttaacaa taatttcttt ttcactaaac
      841 caagaccata taaaaaatgt aactattagt gctattatta tgtataaaaa tataaacatt
      901 aacttattag gtttagactc cattgcttgt ctactatcac ttaaattttc aatattatct
      961 atcttaaact tcaacttcaa caacctcctt aacttgaact tgttcaatgt tatctacttc
     1021 tggaagttgt tctttccata attcataata tctacccttt ttatctaata attctttgtg
     1081 attacctttc tcttttattt cacctttttc taaaatatag atattatcac atcttttaat
     1141 agtacttaat ctatgagcta ttattatagt cgtcatacct ttactaaatt catgaatggt
     1201 cctttctata gccttttcgg ttatagaatc aagattactt gtagcttcat ccattataag
     1261 tatttcagga tttctaagta ttgctcttgc aatagataat ctttgctttt gtcctcctga
     1321 gaaatttgaa ccattctctt caactaatgt gttatacctt aatggtaatg aatttataaa
     1381 atcatgtatt tgagcctttt tacaagcttc tataatctct tcataagtta aatacggatt
     1441 tccaagcatt aagttttcta ttatagttcc attaaataaa aacgtttctt gtgatatata
     1501 ggctatctta tccctaagag tttccacatt aatatctaat aaattataat catttactat
     1561 aatttcacca ttttcacatt gataaaaatt aagtaatagc ttcgctagcg ttgtttttcc
     1621 tgatccactt tctccaacca atgctattct ctcaccttta tcaatagtta tatttatgtt
     1681 ttttaaaatt aattgtctag ttccatacct aaagtcaaca tttttaaact ctatttttcc
     1741 ttttaaagtt tctggattaa tctttttata ctcttcttct gatttttcaa tctctaaatc
     1801 aagtatctca cttaatcttt cagcagctac aacagcagtc tgaagtgtag gctgaagatt
     1861 tattatgttt tctactggac ttaaaaaata tgatagtaaa gcattaaaag ttaaaagttc
     1921 accaaaggaa atttttccat ttaatacttg aatagttccc atccataata tagctatagc
     1981 aaaaactccc tttactaaac tttttaaaga tacctgaatg ttatttatgt actcattttt
     2041 aaatatagct tttagaaact ttataaatct ttcctcggtt tctaaattaa cttctctttc
     2101 tgcattaaaa gcctttatag ttgctattcc atttaaagat tctactaagt atgatgttaa
     2161 cttagcatta ccttccatag tttctctatt aactttttct atattacttt taaatataaa
     2221 tacaataatt ccatataaaa ttaaaggtac tatagttatt ctaaataata atgaactctg
     2281 ataataaaga attcctcctc cgactataac cataatcaca tcaatcatca tagtaagagc
     2341 aactcctgat atagcttatc ttatctttga tgcatcatta aaccttgata taatttctcc
     2401 aacttctctt gtaccaaaga aattcatagg aagattaacc acatgatcat aataacctag
     2461 cattaatggt atatctaaac gttgtcctaa atgaataaga agttgtgttc taaaagcttc
     2521 tgtaataacc ttaaatattg ctaaaagtat aaatcctata gaaaatatag ttaatgattg
     2581 tcttaaatta ttaggtacta tgtcatccaa caaaaattta aaataaaagg accctattat
     2641 tccaaatgcc gttataagaa gtgaagctag aaatatgtta aaaagtaatc ctttttgtgg
     2701 ctttaataaa tcaaaaaacc tagtaaatac tcctttaact tcatttcctt ttttaaattc
     2761 agcggtcggc gtcattatta aaaggattcc tgtccaaata ttaaagaagt cactaggttt
     2821 atatttaact attcctttag ctggatcagc aactataatt tcttttttac taattttatg
     2881 tataactaca taatgtagca ttgtcttgtc tataacaaca tgagctatag ctggaagtgg
     2941 aatttctcca aatatatctt cgggtttatt agctttaact cctttagatg aaaatccaag
     3001 tttttcagca gcctgtataa cgcctaaagc acttgtaccc tctttatctg ttccagctac
     3061 ttctctaatt ttagatattg gaaattttaa tccatattgc ttacatatag tagctaaaca
     3121 tgcacatcca caatccttat agtcatgttg ttttatacac ttatacctat taatatacat
     3181 ctcctccttt taacgcaatt gtatattttc ttttttaacc atatattata tattaattta
     3241 attatacatt ttagtgtata atttgtaaat aattgttgac tatcatttac taagatgtta
     3301 ttatttattc ataaactaat tatttggagg tcttgagaat ggaaaattta aatttaaatc
     3361 aattagaaaa tattaatggt ggttattgga aaacaatttg ggcagttggt ccaggattat
     3421 atcaacgtga tactgaaact ggtaaatatc gttggattca aacacaagat aacctttctt
     3481 atactacaaa cgttattgct aatggttggg ctggatctgc tgctggagga tattttagcg
     3541 gaaggtagga atttattttg aataaaaaag aaaaaagaat agcaatgcaa aatgatttaa
     3601 tatcaagagt ttatgatttg attttagatg aaaaaacacc aaatgatgaa aaatctaaat
     3661 taattgagtt taaaaattct gttgaaaatg gtaaagattt cgaaaaagaa ataatgcatt
     3721 tagcagaaaa tttaagaaaa ttatcacttg taaaatttaa caataaagaa aagcttagct
     3781 ctgaagttgg taaactttac atggatattt cttctactgg atttataaga aacgcattag
     3841 gtcgtggaat agttaatctt agtttcttag ctggcaaata ataaatcaag aaaagaagaa
     3901 tctcgatttt tcaaattaaa aaatcttg
//
LOCUS       DAEVGI010000180         3921 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEVGI010000180 DAEVGI010000000
VERSION     DAEVGI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.182"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            519..1289
                     /locus_tag="K8M77_002293"
     CDS             519..1289
                     /locus_tag="K8M77_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086750.1"
                     /translation="MGKEVLKVSNIEKYYGKKGNVTKAINDISFTVSEGEFVGIMGAS
                     GSGKTTLLNCISTIDKVTSGHVYLEGEDVTSFSSKKLAKFRREKIGFIFQDFNLLDTL
                     TAYENIALALTIQRVNNKEIDKRVRLVAEALNISNELNKYPYEMSGGQKQRVASARAI
                     VTNPSLVLADEPTGALDSKAATMLLESLEDLNKNLNATIMMVTHDAFTASYANRILFI
                     KDGKIFNELIKGTDSRKEFFNRIIEVITLLGGDSNNVF"
     gene            1279..3258
                     /locus_tag="K8M77_002294"
     CDS             1279..3258
                     /locus_tag="K8M77_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI7086751.1"
                     /translation="MYFKLAFKNIKKSYKNYVIYFLTLIFGICIFYTFNSIESQSVMM
                     ELNEQKQSAFMMAEQLIGYFSVFIAFVLGFLIVYANNYLIKRRKKEFGIYMTLGMENG
                     SLSKMIFLETLFIGAISLGIGVVLGIMLSQALSVLTAYMFQVDLTKFQFVFSPLGFKR
                     TVLCFSIIYLVVLIFNFISVRKIKLIDLLTASKRNEKPTIKNLWVSVILFLVSVGILG
                     IAYYKVIHDGIAFASFNALGLPILLGCIGTFIFFYSLTGFFLKVIQGNKKFYLRDLNM
                     FVMKQISSKINTTFVSLSFICLMLFLAICTFSGGLGINRAINADLKDLTKFDVTFWSN
                     SGENIESLLKEKNIDISNIAKEDSNMVMYDSEVKYSDFLSKEGMTAMKNYFPVANDND
                     TLVIGENGYNNTLKLLGKEPVNLKENQYLAVGNIDEMKKWVNESLESGKKINISGKTL
                     EPANKKYENINLYNFTMKGDILILVVKDFLLEGLKPVSSRFNMMLKDNSNTKEELENV
                     RDKLVESQVYSITKKEIYDNAAGLGATMAYLGIYLGLIFIITSAVVLAIQQLTESTDN
                     VERYRLLKEIGVDQKMINKAIFTQVGVYFMLPLSLAIVHSIVGLKISSTIVGVFGNAS
                     IIPNIIITAIIFVIIYGGYFLATYLGAKKNINERS"
     gene            3547..3759
                     /locus_tag="K8M77_002295"
     CDS             3547..3759
                     /locus_tag="K8M77_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBI7086752.1"
                     /translation="MEQLNRKEIIELVNIIRNPKEEWSEAMIDELIFKLERNVIYPNP
                     SDLIFYTELSAEEIADKILDYKPILL"
BASE COUNT         1527 a          362 c          661 g         1371 t
ORIGIN      
        1 ttaatttagt tcatgatttt catataaata tttatatgaa aattaaggag aggtattaaa
       61 ttaagttttg ataatagtag tgaaaaaagg tggattcaat ttatgaatcc acctttttat
      121 ataacattat aatatatttt aataggttag ctaaattttt tataaaaagg ttttagaact
      181 ttttgtatgg actaggtttg ttgtttaatg gaaactaaag tgataatagc aaagttaaga
      241 taataaaggg aatctagcac ctttagaagc tgaataaaaa ataggcttaa ggacgtatgt
      301 tcaatctaat tgttttgcag gttactctaa tctttacata agaatatttt aaaaatcaca
      361 atgtgacact tttgttagtt tagtgttata tagttctatg gattgaaatt tttatatgaa
      421 atagaattaa agtatagaaa gaagagttta aatagataat tttaagcttt tgattagagt
      481 tttaagataa tataagtaag gataaggagt gtagtctaat gggtaaagag gttttaaagg
      541 taagtaatat agaaaagtat tatggtaaaa aaggaaatgt tacaaaggcc attaatgata
      601 ttagttttac tgtttcagaa ggggagtttg ttggaataat gggggcttca ggaagtggta
      661 aaacaacact tttaaattgt atatcaacca tagataaggt aactagtggt catgtatatt
      721 tagagggaga ggatgttaca agctttagtt caaaaaagtt agctaagttt agaagagaaa
      781 aaatagggtt tatatttcag gattttaatc ttttagatac cttaacagct tacgaaaata
      841 tagccttagc tcttacaatt caaagagtga ataataagga aatagataaa agagtaagac
      901 tcgttgcaga agcattaaat ataagtaatg agcttaataa atatccttat gaaatgtcag
      961 ggggacaaaa gcaaagagtg gcatcagcaa gggcaatagt tactaatcca tctttagtat
     1021 tagctgatga accaacagga gccttagatt caaaggcagc taccatgctt cttgaaagct
     1081 tagaagattt aaataagaat ttaaatgcaa caattatgat ggtaactcat gatgctttta
     1141 cagcaagtta tgcaaataga attttattta ttaaggatgg aaagatattt aatgaactta
     1201 taaaaggaac tgattctaga aaagaatttt tcaatagaat aattgaagtc ataactcttc
     1261 ttggaggtga cagtaataat gtattttaag ttagccttta aaaatataaa aaagagttat
     1321 aaaaattatg taatatattt tttaaccttg atatttggaa tatgtatatt ttatactttt
     1381 aactccatag agtcacaaag tgtaatgatg gaacttaatg agcaaaagca gtcagccttt
     1441 atgatggcag aacaattaat aggatatttc tctgtgttta tagcttttgt cttaggattt
     1501 ttaatagttt atgcaaataa ttatctaata aaaagaagaa aaaaagagtt tggaatttat
     1561 atgactcttg gaatggaaaa tggaagctta tcaaaaatga tattcttaga aactctattt
     1621 ataggggcta tatctttagg aataggagtg gtactaggaa taatgttatc tcaagcactt
     1681 tcagtactta cagcctatat gttccaggta gaccttacaa agtttcaatt tgtgttttct
     1741 ccacttggat ttaagagaac agtactttgt ttttcaataa tatatcttgt tgtattaatt
     1801 tttaacttta taagtgttag aaagattaaa ttaatagatc tattaacagc ttcaaagaga
     1861 aatgaaaagc ctactattaa aaatctttgg gtatcagtaa tattattttt agttagtgtg
     1921 ggaatcttag ggatagctta ttataaggtc atacatgatg gaattgcctt tgctagtttt
     1981 aatgctcttg gattaccaat tttactagga tgtattggaa catttatatt cttttattct
     2041 ttaacaggat tctttttaaa ggttattcaa ggaaataaaa agttctattt aagagattta
     2101 aacatgtttg ttatgaagca aataagcagt aaaataaaca caacctttgt atccttatca
     2161 tttatatgct taatgttatt tttagctatt tgtacattct caggagggtt aggaataaat
     2221 agagctataa atgctgactt aaaggattta actaaatttg atgttacctt ttggagtaat
     2281 agtggtgaaa atatagaaag cttattaaag gaaaaaaata ttgatatatc taatatagct
     2341 aaagaagatt caaacatggt tatgtatgat agtgaagtta aatacagtga tttcttatca
     2401 aaagagggaa tgactgctat gaagaactat tttccagtgg ctaatgataa tgatacatta
     2461 gtaattggtg aaaatggata caataataca ttaaaacttc ttgggaaaga gcctgttaat
     2521 ttaaaggaaa atcaatactt agcagttgga aatattgatg aaatgaaaaa gtgggttaat
     2581 gagagcttag aaagtggaaa gaagataaat atttcaggaa aaaccttaga gcctgcaaat
     2641 aaaaagtatg aaaatattaa tctatataat tttactatga aaggtgatat tttaattttg
     2701 gttgttaagg attttctcct tgaaggatta aaacctgtgt ctagtagatt taatatgatg
     2761 ctaaaggata actctaatac aaaggaagaa ttagagaatg taagagataa gttagtagaa
     2821 agccaagttt attcaataac aaaaaaagaa atatatgata acgcagctgg cctaggtgca
     2881 acaatggctt acttgggaat atacctaggt ttaatattta taataacatc agcagttgta
     2941 cttgctattc agcagcttac agaatctact gataatgtag aaagatatag attactaaaa
     3001 gaaataggtg tggatcaaaa gatgataaat aaagctatat tcactcaagt aggagtatat
     3061 tttatgcttc ctttatccct tgctatagta cattcaattg ttggtttaaa gatatcttct
     3121 accatagtag gggtatttgg aaatgctagc ataataccaa atataattat aacagcaata
     3181 atttttgtta taatttatgg agggtacttc ttggcaacat atttaggggc taagaaaaac
     3241 ataaatgaaa gatcatagat atactgtttg taagttttat aaaaagttat ttttatacac
     3301 tccattttta tttattaggg ttaaattttg aaaggaaata actttttata tcatataaaa
     3361 taaattgaag cttgattttt tctaataagt tctaatgtga tgaattataa aaatttttaa
     3421 tatgatttat tgatagaata attattaaaa tatatattat ggtgaataaa atagataaat
     3481 tataaaatat tgtaacatat atgtataaga atattaatta ttttattaaa aggaggtata
     3541 gtaaaaatgg aacagcttaa cagaaaagaa ataatagaat tagtaaatat tattagaaat
     3601 cctaaggaag aatggtcgga agctatgatt gatgaactaa tatttaaatt agaaagaaat
     3661 gttatttatc caaatccaag tgatttaatt ttctatactg aattatctgc tgaagaaatt
     3721 gcagataaaa tattagatta taaaccaata ctattataaa taaaaatcta ttatagatta
     3781 ttgaaaaaaa gtaggtattt aattaagtac ctactttttt gtatttataa ttaaattttg
     3841 agaatattaa tccatagatt atttaaattt aataaatatt agaataattt atatgttaaa
     3901 ttattaacaa aatcattgat t
//
LOCUS       DAEVGI010000181         3916 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEVGI010000181 DAEVGI010000000
VERSION     DAEVGI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.183"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            231..1004
                     /locus_tag="K8M77_002296"
     CDS             231..1004
                     /locus_tag="K8M77_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HBI7086753.1"
                     /translation="MIDIHSHIIPNIDDGSKSLEMSLEMLKRAEESGTKKIIATPHFF
                     KGYWTENYNSVKDKVKELNNLAKENNLNIEIYPGQEVFFNNYILEDFKLGDIGTLGGS
                     RYMLIEFPMDKIYFQKAIDEIYELKIKGITPVIAHPERYVDFINNPDTINQFIDEGYL
                     FQLNGGSLTGLFGKEVKKTAELFLENNLYSFIGSDAHSNGNRNTSLKEACEVANSISR
                     GASRRFEANGLALLNNEKIVFQGQKIKKKKKGLFSFLKR"
     gene            complement(1171..1617)
                     /locus_tag="K8M77_002297"
     CDS             complement(1171..1617)
                     /locus_tag="K8M77_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086754.1"
                     /translation="MIFLCIFLILLGVCTVAFVLYQCNIKITEYKRQILTLNNQLSKY
                     KDTTYKKSNNDSQKTLIINFNKPEFRYGITLPYTSIFIAPSEESIAISKIKEKSQVKI
                     IDEAEINNEIWYYSLLNTESKINCNGWIKKSQFSMLMEDTNNVLKE"
     gene            1755..1940
                     /locus_tag="K8M77_002298"
     CDS             1755..1940
                     /locus_tag="K8M77_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086755.1"
                     /translation="MANLNELELQNLRHLIGAHSTIEKKLECYSEQCTDPTLKNMLKK
                     DAQDAKNSKEKLMSFLG"
     gene            1953..2201
                     /locus_tag="K8M77_002299"
     CDS             1953..2201
                     /locus_tag="K8M77_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBI7086756.1"
                     /translation="MQEKEMISDYLAGINASLAGYGGIISQCENQELRETIQNMRNQD
                     EVRQYALFKIAKEKGYYIPAQQATPEEVATVKQQVSQG"
     gene            complement(2299..3030)
                     /locus_tag="K8M77_002300"
     CDS             complement(2299..3030)
                     /locus_tag="K8M77_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBI7086757.1"
                     /translation="MDNKISKLKQIIENSNNIVFFGGAGCSCESGIPDFRSSNGLFNE
                     KLNITFTPEQLVSHSFFERYPEEFFNFYKAKLIYPNAKPNDAHIALAKLEEMGKLKAI
                     VTQNIDGLHQMAGNKNVFELHGSVLRNYCVDCHTFYDEKFILESKGVPKCTKCGGIVK
                     PDVVLYEEPLDDNVIRGAIDAISKADTLIIGGTSLVVYPAAGLINYFRGKNLVLINKS
                     STQADSKADLVINESIGKVLGKIID"
     gene            3323..3829
                     /locus_tag="K8M77_002301"
     CDS             3323..3829
                     /locus_tag="K8M77_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBI7086758.1"
                     /translation="MFKINDYIMYGTVGVCQVIDITKETLMNNIEKEYYVLSPVYSKY
                     PKKTVIKIPVDNKKISMRTLLSKEDVNSIINSIPETETLWIDNDRQRNDEFKTILRSG
                     NCDDLIVLIRSIYLDKKKRKLDGKKACKGDDEIMQTAEKLIDEEFAVILDIRPEEVKS
                     YIKSHIPQ"
BASE COUNT         1550 a          450 c          581 g         1335 t
ORIGIN      
        1 cttagaatat aatttgctac agctatttgt tttttattaa gataatattt cctatgaaat
       61 atatgtatgt agaatgtatt atttggtttt gtattatggt aaaatattca tgtaaaaaat
      121 agaaagttaa gagtagatta gtaaagggtt ttatattata gtaaaaatta tttaaaccct
      181 atagaggaag atagatttcc ttttaggtat ttattttaag gaggaaaacc atgatagaca
      241 tacattcgca tataatacct aatatagatg acggttcaaa aagtttagaa atgtccttag
      301 aaatgttaaa aagagcagaa gagtcaggaa ctaagaaaat aatagcaact cctcacttct
      361 ttaagggata ttggacagaa aactataaca gtgtaaaaga taaagttaaa gagttaaata
      421 atttagctaa ggaaaataat ttaaatattg aaatttatcc aggacaagaa gtattcttta
      481 ataattacat attagaggat tttaagcttg gtgatattgg aaccttaggg ggatcaagat
      541 atatgcttat agaattccct atggataaaa tatattttca aaaagcaata gatgaaatat
      601 atgaacttaa aataaaagga ataactccag tgattgctca tccagaaaga tatgttgatt
      661 tcataaataa tccagataca ataaatcagt ttattgatga gggatactta ttccaattaa
      721 acggaggaag tcttactggt ttatttggaa aggaagtaaa gaaaacagca gagctattct
      781 tagaaaataa tctatatagc tttattggat ctgatgctca tagtaatgga aatagaaaca
      841 ctagcttaaa ggaagcttgt gaagttgcaa atagtataag cagaggagct agtagaaggt
      901 ttgaagctaa tggacttgct ttattaaata atgaaaagat agtattccaa ggacaaaaaa
      961 ttaaaaagaa gaaaaaagga ttattttcat tcttaaaaag atagatctta tataatataa
     1021 ctaatttatt ttcaaagggt atgtatcaaa ataacttttt ataaaactta caaacagtat
     1081 aaagagaata tatagtaatt ctctgaatat gtttgtaagt tttattatta tattttgata
     1141 cagcccattt tttagtttta aaaataaaat ttattcttta agaacattgt tggtatcttc
     1201 cataagcatt gaaaattggc tttttttaat ccaaccatta caatttattt ttgattctgt
     1261 atttaacaat gaataatacc aaatttcatt gtttatttca gcttcatcaa taatttttac
     1321 ttgtgatttt tctttgattt tactaatagc aatagattcc tcagagggag ctatgaaaat
     1381 tgaggtataa ggtagtgtaa ttccatatct aaattcaggc ttattaaaat ttattattaa
     1441 agttttttgg gaatcattgt tacttttttt ataagttgta tctttatatt ttgaaagctg
     1501 attatttaaa gttaaaattt gccttttata ttctgtaatt ttaatattgc attgatatag
     1561 tacaaaggca acagtacaca cacctaatag tattaaaaaa atacataaaa agatcaaatt
     1621 aaattactcc tttatattag tgtttaatat aatatattgt ttaaacataa gattaattac
     1681 ataattttgt atgaataaat taaaaaaaaa agcttaaact aatagagaat tttaaaatgt
     1741 taggagggat tattatggct aatctaaatg aattagaatt acaaaatctt cgtcacttaa
     1801 taggagctca ctctacaata gagaaaaaac ttgaatgtta ctcagaacaa tgtactgatc
     1861 caacattaaa aaatatgtta aaaaaagatg cacaagatgc taaaaacagt aaagaaaaat
     1921 taatgtcatt tttaggatag gaggaaatca acatgcaaga aaaagaaatg ataagcgatt
     1981 atcttgctgg aataaatgca agtttagcag gatatggtgg tataatttct caatgtgaga
     2041 atcaagaatt aagagaaact atacaaaata tgagaaacca agatgaagta cgtcaatatg
     2101 ctcttttcaa aattgcaaaa gaaaaaggat attatattcc agctcaacaa gcaactccag
     2161 aagaagtagc tactgtaaaa caacaagttt ctcaaggata aaattatcta attaagaata
     2221 ctacatagaa atttgttaaa taaacttaga aagagactat taattttttt atagtctctt
     2281 ttttatttaa aaaataaatt agtcaataat ctttcctaat acttttccta tggattcatt
     2341 tataactaag tcagctttag aatcagcttg agttgaactt ttatttatta aaactaaatt
     2401 ctttcctcta aaatagttaa taagccctgc ggcaggataa acaactaagg aagttccgcc
     2461 tattataagg gtatctgctt tagaaatagc atcgatagca cctctaataa cattatcatc
     2521 taaaggctct tcatataaaa ccacatcagg tttaactatt cctccacatt tagtacactt
     2581 tggtactccc tttgattcaa gaataaattt ttcatcatag aaagtgtggc aatctacaca
     2641 gtagtttcta agaactgatc catgaagttc aaaaacattt ttgttccctg ccatttgatg
     2701 aagtccatct atgttttgag tgactatagc ctttaatttt cccatttctt caagttttgc
     2761 taaagcaata tgagcatcat taggcttggc attaggataa ataagtttag ctttataaaa
     2821 gttgaaaaac tcttctggat atctctcaaa aaaactatga gaaactaatt gttcaggagt
     2881 aaaagtgata ttaagttttt cattaaataa tccattagaa cttctgaaat cgggaatacc
     2941 actttcacag ctacagccag cccctccaaa gaaaactata ttattgctat tctcaattat
     3001 ttgttttaat ttacttatct tattatccat gaaatcatct ccaaatattt gtttataatt
     3061 taattataat ttatttacaa taatatacaa cattcatgat ttataatgat tcatttttaa
     3121 aaataaaata tttccttaca ttaaatattt ttaatgtggt aaaatagaag tatacagcgt
     3181 gtagttttaa tgacgtaatt ccagttgtta aaactacttt ttttatttta gaaaagtata
     3241 gtgctaacta cttgagttaa aagaacaatc tcttaatgat gagatgaaag caaaatttta
     3301 atttgaggaa ggtgaaaatt tattgtttaa aattaatgat tatataatgt atggaaccgt
     3361 tggggtttgt caggttatag atattactaa agaaactctt atgaataata ttgaaaaaga
     3421 atattatgta ttaagtccag tctattctaa gtatcctaaa aagacagtaa ttaaaattcc
     3481 agttgataat aagaaaattt ctatgagaac tcttctttct aaggaggatg taaattccat
     3541 tataaatagt atcccagaga cggaaacttt atggatagat aatgatagac aaagaaatga
     3601 tgagttcaaa acaatactta gaagtggaaa ttgtgatgat ttaattgtat taataagaag
     3661 tatatattta gataaaaaga aaagaaaatt agatggtaaa aaggcatgta agggcgacga
     3721 tgagattatg caaactgcgg aaaaattaat agatgaagaa tttgcagtta tccttgatat
     3781 tcgtccagaa gaggtaaagt cttatataaa aagtcatata cctcaataaa gcataaagaa
     3841 aagtaatcaa gaagttagtg aatactttga tgaaatagta taaaagtgga aattaataag
     3901 ggcgtatatc aaaata
//
LOCUS       DAEVGI010000182         3915 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEVGI010000182 DAEVGI010000000
VERSION     DAEVGI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.184"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(141..941)
                     /locus_tag="K8M77_002302"
     CDS             complement(141..941)
                     /locus_tag="K8M77_002302"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086759.1"
                     /translation="MIEIIKFIFYILSTGFVVFNILDNILIESIKDRYSKVPGKFSNF
                     GKVICFLRVIFIVMIIVEYILSLITLFNRHFKVQYTCNIYFVIAFAIFLEVFLIMYYA
                     FINYFKVKSIFINTLEGNEKADDKNLINMLFPKNEEVSIEKWESKIYKNINRANLIST
                     TLSGIYLLVIIHLILQGDLFNDMNLFSILLILLIANVVASSILASLKKALQEVIDNKE
                     YIFNYGENEYIKTKLYLDYKDEYLIIKDGYEIFIPKSNVKRKVVRTIE"
     gene            1453..1671
                     /locus_tag="K8M77_002303"
     CDS             1453..1671
                     /locus_tag="K8M77_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBI7086760.1"
                     /translation="MKLHEAKILSTVKVIDIKAQGELRRRFFDLGIIEGTDIKVLYGG
                     PFGDPRAYLIRGTVIAIREEECEEIEVI"
     gene            1689..3812
                     /gene="feoB"
                     /locus_tag="K8M77_002304"
     CDS             1689..3812
                     /gene="feoB"
                     /locus_tag="K8M77_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBI7086761.1"
                     /translation="MSLNKNSTGISLLSKEKSKGNTKEDYVVALAGNPNVGKSTVFNA
                     LTGLNQHTGNWPGKTVAKAEGYYEFKDSTIKIVDLPGTYSLLSNSEEEEIARDYICFN
                     DPDIVVVVADATSLERNLNLFIQISEITEKAILCVNLIDEAEKNNIKIDLDLLSKELN
                     SNLVSSSARNGVGIEELKEAIFKEITLKDSKKNAINYDSPIEKAIEEIEIILDETLEV
                     PKRKKRWIALRILEGNTSILKSLYNKYNIQEKYINMISKIKDELNKNYKDELVEDIII
                     KSIIKEAERIGNKVVKGGEEYTDFQRKIDKILVSKSTGIPIMIMTLLIVLWITITLAN
                     YPSEMLANMFAHGEIYIRDFFSGLNLPPWISGILIDGIYVTLAWVISVMLPPMAIFFP
                     MFTLLEDLGYLPRIAFNLDKCFKKCCACGKQALTMCMGLGCNAAGIIGCRIINSPRER
                     IIAILTNAFMPCNGRFPMLISIAAIFIGGISVGIKESFISALTVTVVIILGVLMTLLV
                     SKILSKTILKGMPSNFILELPPYRKPQVGKVIVRSIFDRTLYVLGRAIAIAAPAGAVI
                     WIFSNIMICDASILTICADYLSPLANAIGVDGYILMAFILGLPANEIVMPIIIMSYLR
                     ATTMLELDNLYELKELLVANGWTILTAINVMILCLMHYPCGTTLWTIKQETKSFKWTA
                     LSFLIPTVAGIVICFITTQIWRLFA"
BASE COUNT         1538 a          609 c          513 g         1255 t
ORIGIN      
        1 ggctgtgaag gcactattat tactaatata aataaaattt ctataattct tcttaaaaac
       61 tttttattcc tcattaacct cttcctttca tcataccttc ttatattgca ctgtaaaaag
      121 ccaaatctat atacgtactt ttattcaata gtccttacaa cttttctttt aacattactt
      181 ttaggtataa atatttcata tccatctttt attattaaat attcatcctt ataatctaga
      241 tataatttag tttttatata ctcattttcc ccataattaa aaatatattc cttattatct
      301 attacttctt gcaatgcttt ttttaaacta gcaagtatag aagaagctac tacatttgct
      361 attaataaaa ttagaagaat agaaaataaa ttcatatcat taaataaatc accttgtaat
      421 attaaatgta taataactaa taagtatata ccgcttaatg tggtactaat taaattagcc
      481 ctattaatat ttttatatat tttactttcc cacttttcta tacttacttc ttcattttta
      541 ggaaatagca tattaattaa atttttatca tctgcttttt cattaccttc tagagtattt
      601 ataaaaatac tttttacttt aaaatagtta ataaatgcat agtacataat taaaaagact
      661 tctaaaaaaa tagcaaaggc aataacaaag tatatattac aagtatattg aactttaaaa
      721 tgtctattaa acaatgttat taaacttaaa atatactcta caattatcat aactataaaa
      781 ataactctta aaaaacaaat aactttacca aaattcgaaa acttccctgg aactttacta
      841 tacctatctt taatactttc aattaaaata ttatctaaga tattaaagac tacaaaccca
      901 gtagaaagta tataaaaaat aaactttata atttctatca ttttatcctc cgtgtacaaa
      961 atattttaaa atgctctttc tcaaagtcta taacaaccta ctatttctca ttaaaccctt
     1021 taattataaa atcactccat aaaccctcag aatgttacta atattatata acaaaactgt
     1081 taataattta aatttattac tctttattct tataatcttt ccctataaat ttttataact
     1141 ttctttaatt acatactcat ttaaatcaag caaataactc atatatgtaa gataaagctt
     1201 tataactttt ttcaaaatta tacactcact taaaaaaaat ataactaaag tctcccttta
     1261 agcttaaagc atagtctcta acccccttat taatccacat atacaaaccc tctcaatcct
     1321 ccctaaagtt tcaatcacac taaattatta ttcatcatag tttattatat atggacagta
     1381 ctgttaatta ataaaagccc taaacttctt tcataaaaat aatcttagat taagccttag
     1441 gagggaataa aggtgaaatt acatgaagct aaaatattgt caactgttaa ggtaattgac
     1501 ataaaggcac agggagaact aaggagaaga ttttttgact taggaataat agaaggaaca
     1561 gatataaaag ttttatatgg tggccctttt ggtgacccta gagcctattt aataagagga
     1621 actgtaatag ccataaggga agaagaatgt gaagagatag aagtaattta atagtaggag
     1681 aaaaagatat gagtttaaac aaaaattcta ctggaattag cttattatct aaagaaaaat
     1741 caaaaggaaa caccaaggaa gactatgttg tagcattagc aggaaacccc aatgtaggta
     1801 aaagtacagt ttttaatgct ctaactggtt taaatcaaca tactggaaac tggcctggaa
     1861 aaacagtagc taaggcagaa ggatactatg aatttaagga ttcaaccata aagatagtag
     1921 atttacctgg aacatactca ttgctatcaa attcagaaga agaagaaatc gcaagggatt
     1981 atatatgctt taatgatcca gatatagtgg ttgttgtagc tgatgcaacc tccttagaaa
     2041 ggaatttaaa cctttttata caaatatctg aaataacaga aaaggcaatc ctctgcgtaa
     2101 acttaattga tgaagcagaa aaaaataaca taaaaataga tttagatttg ttatcaaaag
     2161 aactaaactc aaaccttgtt tcctcctctg ccagaaatgg agttggcata gaagaattaa
     2221 aagaagccat ctttaaagaa atcaccctaa aagactccaa aaaaaatgca ataaattatg
     2281 attcccctat agaaaaagca atagaagaaa tagaaatcat actagatgaa acccttgaag
     2341 ttcctaaaag aaagaaaaga tggatagctc ttagaattct agaaggaaat actagtatct
     2401 taaaatcctt atacaacaaa tataatattc aagaaaagta tataaatatg ataagtaaaa
     2461 taaaagatga gcttaataaa aattataagg atgaattagt agaagatata atcataaaat
     2521 ccataataaa agaagctgaa agaataggaa ataaggttgt taaaggggga gaagaatata
     2581 ctgacttcca gagaaaaata gataaaatac ttgtgtcaaa atcaacagga atacctatta
     2641 tgataatgac cctattaatt gtcttgtgga taaccataac cttagcaaat tacccatctg
     2701 aaatgcttgc taatatgttc gcccatggag aaatttacat tagagacttc ttctctggac
     2761 taaacttacc tccttggata tcaggaatat taatagacgg catttatgtt accttagctt
     2821 gggtaatttc agtaatgcta cctcctatgg caattttctt ccctatgttc actctattag
     2881 aggacttagg atatttgcca agaatagcat ttaacctaga taaatgcttt aaaaagtgct
     2941 gtgcctgtgg taaacaagcc cttaccatgt gtatgggact tggatgcaat gcagcaggaa
     3001 tcataggatg tagaataatt aactctccta gagaaaggat tatagctata ttaactaatg
     3061 cttttatgcc ttgtaatgga agatttccta tgctaatttc catagcagcc atattcatag
     3121 gtggaatatc agtaggaata aaagaaagct tcatatcagc tcttacagta actgtagtta
     3181 taattttagg agtcttaatg acattactag tatcaaaaat cctatcaaag acaatactaa
     3241 aaggaatgcc ttcaaacttt atattagaac tacctccata tagaaaacct caagtaggaa
     3301 aggtcatagt aagatcaata tttgatagaa ctctatatgt tttaggtaga gccatagcaa
     3361 ttgcagctcc agcaggggca gttatatgga tattctctaa tataatgatt tgtgatgcct
     3421 caatcctaac aatatgtgct gactatttaa gcccacttgc taatgccata ggggtagatg
     3481 gatatatatt aatggccttt atcttaggat taccagcaaa tgaaatagta atgcctataa
     3541 taataatgag ctatttaaga gccaccacaa tgttagaatt agataaccta tatgagctta
     3601 aggaattatt agttgctaat ggatggacaa tcctaactgc aattaatgtt atgatactgt
     3661 gcctaatgca ctacccttgt ggtacaaccc tctggacaat taaacaggaa actaaaagct
     3721 ttaaatggac agccctaagt ttcctaatcc caacagtggc aggcatagtt atttgcttca
     3781 tcactactca aatatggagg ctctttgcct aaaaacctaa aaaagccaaa tttaataaaa
     3841 aaagcttaaa actcaagaat taaaaaatca taaaaaatta aaaccataaa atctcaaagg
     3901 ttaaaattct aaaaa
//
LOCUS       DAEVGI010000183         3874 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEVGI010000183 DAEVGI010000000
VERSION     DAEVGI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.185"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            79..1029
                     /locus_tag="K8M77_002305"
     CDS             79..1029
                     /locus_tag="K8M77_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI7086762.1"
                     /translation="MKKKLLSIALSSLVVVSLFIGCSNKKNNENTTVPTNMVSVEHAM
                     GTTEIPTNPQKVVILTSEGTEALLALGVKPVGAVTGVTGDWYDHTKDELEGVKQVGME
                     REPNIEAIAALKPDLIIGNKMRHEKIYSQLSSIAPTVYSDTIRGAWKDNFKFYAKVLN
                     KESESEKALKEYDDKIAYIQNNYKDKLDTEISLIRFMSGKTRIYLGNTFAGTILKEIG
                     FKRPASQQSDEFALEIGKERLKEADGDVLFYFTYETGDGKATAREQEWMQDPLFNSLN
                     AVQNNKVYKVNDFIWNTAGGIKSANLMLDDLTNMLNEGKL"
     gene            complement(1205..1801)
                     /locus_tag="K8M77_002306"
     CDS             complement(1205..1801)
                     /locus_tag="K8M77_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBI7086763.1"
                     /translation="MSKVLYIKANIKNEGESRTFKVSDSFVEEYKKNNPEDQIITLDL
                     YKENIDFLRADDLGKLFGPKDEESKNNSILKYAYQFADADKYIIAAPMWNLSFPAILK
                     AYIDYVSVSGITFKYTAEGPVGLLNNKKAVHIVSRGGGYDNSPYEMGDRYLRTILGFF
                     GIKDIETIDIDNLDVIGVNVKEKVKEGIEKAISLAKKF"
     gene            complement(1835..2470)
                     /locus_tag="K8M77_002307"
                     /pseudo
     CDS             complement(1835..2470)
                     /locus_tag="K8M77_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480536.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
     gene            complement(2602..3105)
                     /locus_tag="K8M77_002308"
     CDS             complement(2602..3105)
                     /locus_tag="K8M77_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086764.1"
                     /translation="MKELRIKKFTVEEFKNKFNLDNIENISAYEVSELGNLFENSSEE
                     VYFLIEDKLYAEPKEAIGEGELSREDIETIEKLNEEFRDSLISLREFLYHGNGKLDSK
                     EARDIQVSMNNNIKLAKEILNRHNIVDKDQLKKMSKKYFMPDFLNGVFGNEEYNFENE
                     VEIDCGL"
     gene            complement(3322..3747)
                     /locus_tag="K8M77_002309"
     CDS             complement(3322..3747)
                     /locus_tag="K8M77_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI7086765.1"
                     /translation="MLEENFCQCLFFTVTRLKKIAAKIAEDELKPLGMSPTYVYILLG
                     VKFKPGITQKELCEELHLKPSTITRLIDKLVINGLVERRSEGKLSHIYLTESGENIQE
                     DIKKYRLKLHNRYKEILGEEEYNFLTKLTNDSARILESE"
BASE COUNT         1336 a          609 c          419 g         1510 t
ORIGIN      
        1 ttatcattta tgatatcata aactcattag tttttctaca tcattcgaca aaaaaataat
       61 tatttggagg ttttaagtat gaaaaaaaaa ttactatcta tagctttatc aagcttagta
      121 gttgtaagcc tatttatagg ttgttctaat aaaaaaaata acgaaaatac aactgtacca
      181 acaaatatgg tatcagttga acatgcaatg ggtactacag aaatacctac taatcctcaa
      241 aaagttgtaa ttcttactag tgaaggtaca gaagctctac ttgctcttgg cgttaaacca
      301 gtaggtgctg taactggagt tactggtgat tggtatgatc atactaaaga cgaacttgaa
      361 ggtgtaaaac aagtcggtat ggaaagagag cctaatatag aagcaattgc tgctttaaaa
      421 cctgatttaa taataggaaa taaaatgaga catgaaaaaa tttattctca actaagttcc
      481 atagctccaa ctgtttactc tgatactata agaggagcat ggaaagataa ctttaaattt
      541 tatgcaaaag ttttaaataa agaatctgaa tctgaaaaag ctttaaaaga atatgatgat
      601 aaaattgctt atattcaaaa taattataaa gataagctag atacagaaat ttcacttatt
      661 agatttatgt ctggaaaaac tagaatatat cttggaaata cttttgctgg tactatatta
      721 aaagaaatag gatttaagag accagcatct caacaaagtg atgaatttgc ccttgaaatt
      781 ggtaaagaaa gattaaaaga agctgatgga gatgttctat tctactttac ttatgaaact
      841 ggtgatggta aagctactgc aagagagcaa gaatggatgc aagaccctct atttaactca
      901 ttaaatgctg tacaaaataa taaagtctat aaagtaaatg actttatttg gaatactgct
      961 ggtggaataa aatcagctaa tttaatgctc gacgatttaa ctaatatgct aaatgaagga
     1021 aaattataat ttataccttt cagtaataaa atcttagata atttcaaaca aatttatccc
     1081 taaatattaa taatactgaa aatgaaattt ttcattttca gtattatttt ttattcaata
     1141 tcacttttta aattaccaaa atttatttta ataaaatatt aacttaaaga tctattattt
     1201 taatttaaaa tttctttgca agtgaaatag ccttttctat accttctttt accttttcct
     1261 taacatttac gccaataaca tctaaattat ctatgtcaat agtttctata tccttaattc
     1321 caaagaaacc taatatagtt cttaaatatc tatctcccat ttcatatggt gaattatcat
     1381 aaccaccacc tctagatact atatgtactg cctttttgtt atttaataaa ccaactggtc
     1441 cttcagcagt atatttaaac gtaattccag aaacacttac ataatcaata taagctttta
     1501 gtatagctgg aaaacttaaa ttccacatag gtgctgcaat tatatattta tcagcatcag
     1561 caaattgata tgcatatttt aatattgaat tattcttgct ttcttcatct ttaggtccaa
     1621 ataattttcc taaatcatca gctcttaaaa aatctatatt ctctttatat aaatctaatg
     1681 ttattatttg atcctctgga ttatttttct tatactcttc tacaaaacta tcagatactt
     1741 taaaagttct tgattctccc tcatttttta tatttgcttt aatatataat actttactca
     1801 ttgtattcac tccttctaat ttatatataa attattaaaa attctctgtt ccaaacttat
     1861 tgtttctaaa atcttcatat gcatcaagta tttctcctag tgtattcata acaaatggtc
     1921 catgtgaaac aacaggctca tttattggtt ctccactaag aactaaaaat agtgactctt
     1981 cagtcaaaga ctctagtaaa atctctccct caacattatc aaatatgata aaatctcctt
     2041 ctttacatat tttatcttca tttactttag cttcaccttt taatataaga attgcagtat
     2101 taaaattttt tggttcactt aatgtagtat taccataatt ctttaaattt atattataaa
     2161 gatttatatg agtaaaggta ttagctggcc cctttacctc attaacttcc cctgcaatta
     2221 tacttatttc tcctttatca ttatcaagtt ttaaaactcc catttcttct tttaataaag
     2281 gttgatattt aggttctatc attttttatc tttagtaata tggtgcattg taatctaata
     2341 ataaaaatgg tgataaacgc tccatacttt ttattcctgt tggtatatat tgagatactc
     2401 taaatccatc tccaaccata tgtggttctg gcgctttaaa tatattttct atagttctga
     2461 acttcttcat ttactttgcc ctttttgtct aaaaaaattt ttaggtattt atcactaatt
     2521 agtgatattg tcaacattaa atataattat ttaaataaaa aagctatatc aaaccaagta
     2581 aagatttgat atagctttct cttataatcc gcagtctatt tctacttcat tttcaaaatt
     2641 atattcttca ttaccaaata caccatttaa gaagtctggc ataaagtatt ttttactcat
     2701 tttctttaat tggtctttat caactatatt atgtctattt aaaatttctt tagctaattt
     2761 tatattatta ttcattgaaa cttgaatatc tctagcctct tttgaatcta attttccatt
     2821 tccatgatat aaaaactctc ttaagcttat taaagaatct ctaaattctt catttaattt
     2881 ttctatagtt tcaatatcct ctctagaaag ttctccttcc cctatagctt cctttggctc
     2941 agcatataat ttatcttcaa ttaagaaata aacctcttca gatgaattct caaaaagatt
     3001 tcctaattca cttacttcat aagctgaaat attttctata ttatctaagt taaacttatt
     3061 tttaaattct tctacagtaa atttctttat tcttaattct ttcatatatg tatatctcct
     3121 ttttgaatat tttaaatcat taaataaata ttctaatctc ttattaaata ataaatttat
     3181 tatatcatta ttaatctaaa ataagtactt atatatataa taatgcacag cttcttataa
     3241 ttaaaattgt aagaagttgt gcattactac ttgttttcaa ataagtttaa acctttaaat
     3301 tttaactatt aagcacatct tttattcaga ctctaatatt cttgcagagt catttgttag
     3361 tttagttaaa aaattatact cttcttctcc aagtatctct ttataacgat tatgtaactt
     3421 taatctgtat tttttaatat cctcttgtat attttctcca ctctcagtta gatatatatg
     3481 agataatttt ccctctgatc ttctttccac caatccattt ataacaagtt tatctataag
     3541 tctagttatt gtagaaggtt tcaaatgaag ttcctcacat aattcttttt gagttattcc
     3601 tggtttaaat tttaccccaa gaagtatata tacatatgtt ggcgacattc ctaatggttt
     3661 taattcatct tcagctattt tagctgctat ttttttcaat ctggttacag taaaaaacaa
     3721 gcactggcaa aaattttctt caagcattat atcagtccta acctcttaaa attttgtcta
     3781 tatattatat taaatttgaa ctactcggca ttgaaatgcc aagatccttg ggcaacacac
     3841 ctaacggtgt gagattaacc aagctaacaa gggc
//
LOCUS       DAEVGI010000184         3811 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEVGI010000184 DAEVGI010000000
VERSION     DAEVGI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.186"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(209..1210)
                     /locus_tag="K8M77_002310"
     CDS             complement(209..1210)
                     /locus_tag="K8M77_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HBI7086766.1"
                     /translation="MALFESYERRINQIQPVLEKYGMETIEDAKVVCENLGIDPYTIV
                     KETQPIAFENAGWAYTLGAAIAIKKGCKTAADAAEAIGEGLQAFCIPGSVADDRKVGL
                     GHGNLGAMLLRDETKCFAFLAGHESFAAAEGAIKIAEKANKVRKEPLRVILNGLGKDA
                     AFIISRINGFTYVETKFDYVTGKLNIVKETPYSNGPRAKVKCYGSDDVREGVAIMHHE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECVEQGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVAVAVAVEEGMNK"
     gene            complement(1244..1936)
                     /locus_tag="K8M77_002311"
     CDS             complement(1244..1936)
                     /locus_tag="K8M77_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HBI7086767.1"
                     /translation="MMYSSEVQEMCVIAKGPNHGPAPIPVEGRWVQSREIKDVSGLTH
                     GVGWCAPQQGACKLTLNVKDGIIEEALIETIGCSGMTHSAAMASEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRSQTAFSEGGLPIGAGLEDLGKGLRSQVGTMYGTLAK
                     GPRYLEMAEGYVTKVALDADDQIIGYRFVHLGRMMEMVAKGMSANEAMEKATGQYGRF
                     DEAVRTIDPRHE"
     gene            complement(2193..2543)
                     /locus_tag="K8M77_002312"
                     /pseudo
     CDS             complement(2193..2543)
                     /locus_tag="K8M77_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-GlcNAcase NagJ"
     gene            complement(2600..2779)
                     /locus_tag="K8M77_002313"
     CDS             complement(2600..2779)
                     /locus_tag="K8M77_002313"
                     /inference="COORDINATES: protein motif:HMM:NF019193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HBI7086768.1"
                     /translation="MPEVDINDYPTVSARGIIEGFYGTTWTHQDRLDQIKFYGKNKLN
                     TYIYAPKDDPYHREK"
     gene            complement(2799..3752)
                     /locus_tag="K8M77_002314"
     CDS             complement(2799..3752)
                     /locus_tag="K8M77_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086769.1"
                     /translation="MTQYKFMRKNLSKYKSYLLTSIYGLTLESSKGGKNYISKESTKK
                     NIDGEVIDLKEKLKRGLEFSNKDIINTKFILERNIEFREILSTEILKSNGLEIVVDRE
                     FPNIVQYKIGEKIFKGARKSRDEFLINGSRFKPKVTSEKIYDHIEGEYYLYRLNFRKI
                     NVEMSISFKIIENTVKLQFEEINENGEFKIYKIQIPNHNLISINEEEEKLDITTARVH
                     GILSSNDYFYDLREKEVDKNPVHQTMAFLDNGRLFASLENNVLNYEEKVYYQTTLNEG
                     IKEVGLWNGSWTYREQLSGSEGGTFSGVTELPWSRILILYL"
BASE COUNT         1096 a          759 c          454 g         1502 t
ORIGIN      
        1 taattataag acaaatcttt ttaaaattcc accctttttg tttactaaat tataattttt
       61 ctatatttta ttatactaaa cattttcaac aaaagtaata tttgtaataa aaaatacacg
      121 ttatgtaaac ttaatattta cataacgtgt actcatttat caaaacttta taatagactt
      181 atatcttccc taagcgttga tatatctatt atttgttcat tccttcttct acagcaacag
      241 caactgcaac agtagctcct accattggat tgttacccat accgataaga cccatcattt
      301 caacgtgagc tggaactgat gatgatcctg cgaattgtgc atctgagtgc attcttccca
      361 tagtatcagt cataccgtat gaagctggac cagcagccat gttatctggg tgaagagttc
      421 ttcctgtacc accacctgat gctactgaga agtatttctt accttgttca acacattctt
      481 ttttgtatgt tccagcaact gggtgttgga atcttgttgg gttagttgag ttaccagtga
      541 ttgatacgtc aactccctca tgatgcatta tagcaacacc ttctctaacg tcgtctgaac
      601 cgtagcattt aactttagct cttggaccgt ttgagtatgg agtttcttta acgatgttta
      661 atttaccagt tacatagtca aatttagttt caacatatgt aaatccgttg attcttgaaa
      721 ttatgaatgc agcatctttt ccaagaccgt ttaatataac tcttaaaggc tcttttctaa
      781 ctttgttagc tttttcagct attttgatag caccttcagc agcagcaaat gattcgtgtc
      841 ctgctaagaa tgcgaaacat ttagtttcat ctcttaaaag catagctcct aagtttccgt
      901 gtcctaatcc aacttttctg tcatcagcaa cagatcctgg aatacagaaa gcttgtaatc
      961 cttctccgat agcttcagca gcatcagcag cagttttaca tcctttcttg attgctatag
     1021 cagcacctaa agtgtaagcc catccagcat tttcgaatgc tattggttga gtttctttaa
     1081 cgattgtata aggatcgatt cctaaatttt cgcaaactac tttagcatct tctattgttt
     1141 ccattccgta cttttctaat acaggttgta tttggttaat tcttctttca taactttcaa
     1201 ataatgccat tttattatcc tccttcaata atgaatatat agattattcg tgtcttggat
     1261 ctatagttct aacagcttcg tcaaatcttc cgtattgacc agtagctttt tccatagctt
     1321 cgttagcgct cattccttta gctaccattt ccatcattct acctaagtga acgaatctgt
     1381 atcctatgat ttgatcatct gcatctaaag ctacttttgt tacatatcct tcagccattt
     1441 caaggtatct tggtccttta gctaaagttc cgtacatagt acctacttgt gatcttaatc
     1501 cttttcctaa gtcctcaaga cctgctccta ttggtagtcc accttctgag aaagcagttt
     1561 ggcttctacc ataaactatt tgaaggaata attctctcat tgcagtattt atagcatcac
     1621 aaactaagtc tgtgtttaaa gcctctaata tggtttttcc tggtaatatt tctgaagcca
     1681 tagcagctga gtgagtcata cctgaacatc ctatagtttc tattaaagct tcttctatga
     1741 taccatcttt aacgtttaaa gttaatttac atgctccttg ttgtggtgca caccagccaa
     1801 ctccatgagt taaacctgaa acatctttta tttctcttga ttgaacccat ctaccttcta
     1861 cagggattgg tgctggaccg tggtttggac ctttagcaat tacacacatt tcttgaactt
     1921 cgcttgaata catcatattc atttctcctc cctaaaaaat aacttatagt aaaaatttac
     1981 tatatctatt cctcttaaca acttgggact ttttttgtcc ctatgggata ttaaattacc
     2041 ctcacgtcta ttttaacaaa acaatggcga tgtaacaata tatttctaaa aatttttcta
     2101 aaaaaactca atattttttt attttattac aatatttgag aataattctc atatcttttc
     2161 ccaaattatt gaaaacaaat tatattttta cctattaatc cttctccatc tccaactgat
     2221 gaattggtta tgcttaaatt gcctccttct gttttagctt ctgctcttag gacaactttt
     2281 gctaaaactt ctttagctgg caatggttct ccagttaaag aacttacaag cactctaacc
     2341 tttccgtctt ctattttctt agcatttaca aacactccat catcagaaat tgcttctaca
     2401 tattcaaagg cattttcatc ataatttagt gtaaaatcat aagcaaaagc ttctgcattt
     2461 actaattcgt ctatacccac tccaacttct aagttttctc caacttgaac ttcctctaaa
     2521 acttcaagag atacacttcc tgagaagcat ttataagttc ttgcattctt tgcattttac
     2581 tttttggata tggatttctt catttttctc tgtgatatgg atcatcttta ggagcataaa
     2641 tatatgtgtt taatttattt tttccataaa acttaatttg atctaatcta tcttgatgtg
     2701 tccaagtagt tccataaaag ccttctataa tccctctagc actaactgtt ggataatcgt
     2761 ttatatctac ttctggcata gtttattaaa ctttcttatt ataaatataa aattaagatt
     2821 cttgaccaag gtaattcagt tactccacta aaagttccac cctctgagcc acttaattgc
     2881 tccctatatg tccatgagcc attccaaagg cctacttctt taatcccttc atttaaagtt
     2941 gtttgataat atactttttc ctcataatta agaacattat tctctaaaga tgcaaataat
     3001 cttccattat ctaaaaatgc catggtctga tgaactggat ttttatcaac ttctttttct
     3061 cttaaatcat aaaagtaatc attagaactt aatatcccat gaactcttgc tgtagtaatg
     3121 tctagttttt cttcctcttc atttatgctt ataagattat gatttggaat ttgtatttta
     3181 taaatcttaa attctccatt ttcatttatt tcttcaaact gaagtttaac agtattttca
     3241 attattttaa aagatattga catctctaca tttatttttc taaaatttaa tctatataaa
     3301 tagtactctc cttcaatatg atcataaatt ttttctgagg ttactttagg tttaaatcta
     3361 cttccattta ttaaaaattc atcccttgat tttcttgctc ccttaaaaat cttctctcct
     3421 attttatatt gaactatatt aggaaattct ctatcaacaa ctatttctaa tccattgctt
     3481 tttaatattt cagtgcttaa tatttctcta aattctatat ttctttctag aataaactta
     3541 gtgtttataa tatccttatt gctaaactct aatccccttt ttagtttttc ctttaagtct
     3601 ataacttctc catcaatatt tttcttagtt gactctttac taatataatt ttttcctccc
     3661 tttgatgatt ctagagttaa tccataaatt gaagttaata aataagattt atattttgaa
     3721 agattttttc tcataaattt atattgcgtc ataatttccc cctatctttc actccttaag
     3781 ttctttttta taaaaactta acagtttttc t
//
LOCUS       DAEVGI010000185         3792 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEVGI010000185 DAEVGI010000000
VERSION     DAEVGI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.187"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(37..231)
                     /locus_tag="K8M77_002315"
     CDS             complement(37..231)
                     /locus_tag="K8M77_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086770.1"
                     /translation="MEIIQKIIKLELENKFPTKDLIPNPPEFMSTVHNGDRDCIEIVE
                     ESSMKIVLDFIINILEELGI"
     gene            complement(556..834)
                     /locus_tag="K8M77_002316"
                     /pseudo
     CDS             complement(556..834)
                     /locus_tag="K8M77_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1084..1887
                     /locus_tag="K8M77_002317"
     CDS             1084..1887
                     /locus_tag="K8M77_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI7086771.1"
                     /translation="MTLSENNIFKLIKNLFSLFLIYIGLQILASLISMTFMGILGLLL
                     KQNLLSKSSNSLNFLVFDCIFQIMAIYFFSKTYKNKEFNIIKINNHISLKTIGKYSLI
                     SLGIGGISTLWILLANFLSNYLPFIKNSLEDFSNLVSVFDGYPFLTIISVVILAPIFE
                     ELVFRGIIFNEASKVKGGIFPILVSGLLFGLFHVQPIQIVYTSILGIILAFVYSKTHS
                     LPIVMFLHMLNNLAAISPESVKPIVMVVQVFCIIPMVYLLKKLYRESKV"
     gene            complement(2107..3054)
                     /locus_tag="K8M77_002318"
     CDS             complement(2107..3054)
                     /locus_tag="K8M77_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086772.1"
                     /translation="MRKNIKSLIGEIFKKGIIVGVCASTLFFVGCVEENKESLDKHKG
                     DNVEDTSEKEKEKDDLKEVLISEDEKRESDKRTLSLASEIINESLNEIKVISRDNTSG
                     KLEDIEFELSSVMEKENKRLENLVEKIYDEALLSTFKDYIKGNELRAKYYKGRSENYM
                     EVMDYGSESDEIISISICKMVDEYGLVINEENMDFYEDCREKVAYLDKKEDYKKIAKE
                     LISKGEFKVELEEDEAIKSGFGEDKEVFKYSKIVENTSGINLDYIAFSINGKLENGNI
                     EEFPSVLIGFNSGKSDEIKFYTTNKYESMEVEVEWVSFN"
     gene            complement(3504..3683)
                     /locus_tag="K8M77_002319"
     CDS             complement(3504..3683)
                     /locus_tag="K8M77_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBI7086773.1"
                     /translation="MDIKEKVLEVLNSSEEPLKGGEIAEKAGIDKKDVDKAIKELKKE
                     EKIISPKRCFYSVNK"
BASE COUNT         1282 a          668 c          279 g         1563 t
ORIGIN      
        1 tttataactc ttatatttaa ctattaattt taagtattaa attcctaatt cttcaagtat
       61 attaataata aaatctaata ctattttcat agaactctct tcaactattt caatacaatc
      121 tctatctcca ttatgaactg tactcataaa ctctggagga ttaggtataa gatcctttgt
      181 tggaaattta ttttcaagtt ctagcttaat aatcttttgt attatctcca catccccttt
      241 agtaactacc ccaatagata tgttagttat attttcagct tcaaaacttc tatcatcaga
      301 acctggagtt ctttttataa tctcaatata ttctttatct aataatcctt cacttattat
      361 ttttttctct ttcattgctc ctaaattctt ttcaggccct atgagcatca tatctccaaa
      421 cccacaagta tcaatattaa tcattgcata tatgttctct cttccagtac taatgatata
      481 ctgttcactt cccctatcct catattcctc tctatcaaaa aatacaagat caataggtct
      541 attaactcta tttaacttaa gatacttggc tacctctatt aaaatactaa ctcccgaagc
      601 attatcattt gctccacaac ttccttcaat attatcataa tgtgcaccaa taaccagttt
      661 aggcaatttt atttcactat ttaaagtaac aattatattg tcaacaaaat gattcctcaa
      721 tttctcatgc tgtattttat gttttaaatc atttttctta agtattttaa ttaaagattc
      781 tctacgttca actttattaa cttttgaaat taatcttaca ttttctaaca tcatattctc
      841 caatacaact tttccatttt aatacaattt tatcataata ctttctataa taatactaaa
      901 attgtatgtt tttaattatc tatttatcaa aattaataaa atatcactaa tttcttattt
      961 attacttgaa aataaagttt ttccttctca ctaccacaca atcgtatatt ttaataatat
     1021 ttgaatttta tgttaaataa tgttaaaata ttacatatat aaaaaattta aggaggatta
     1081 ttcatgactc tatctgaaaa caatatattt aaactaataa aaaatctttt ttcattattt
     1141 ttaatatata taggactaca aattttagca tctttaattt ctatgacttt tatgggaata
     1201 cttggattat tactaaaaca aaatttacta tctaaaagtt ctaatagtct taattttctt
     1261 gtatttgatt gtatttttca aattatggca atatatttct tttctaaaac ctacaaaaat
     1321 aaagaattca atataataaa aattaataat catatttcat taaaaaccat aggaaaatac
     1381 tctttaattt ctcttggaat aggcggaata tccacattgt ggatcttgct tgcaaatttt
     1441 ctatcaaatt atttaccttt cataaagaat agtttagaag acttttctaa tcttgtaagt
     1501 gtatttgatg gttatccatt tcttacaatt atatcagttg taatacttgc tccaattttt
     1561 gaagaacttg tttttagggg aataatattt aatgaagcct caaaagttaa aggaggaatt
     1621 tttccaatac tcgtatctgg cttgttgttt ggtttatttc atgtgcaacc tatacaaatt
     1681 gtatatacct ctattttggg tattatctta gcctttgtat atagtaaaac tcattctctt
     1741 cctattgtaa tgtttttgca tatgctaaat aatttagcag caatttctcc tgaatcagta
     1801 aaaccaattg ttatggtagt gcaagtattt tgcataattc caatggtata tttattgaag
     1861 aaactctaca gagaaagtaa agtttaagtc tctaaaagac actattaata gctaaatttt
     1921 aaggattaca agtcaaaact ccattctaaa ttctcttcaa aacaaaatat tcataaagat
     1981 caagttttta taggttagat ttttgtaata atctcttgcc tataaaaacc cttaacttcc
     2041 tctatcctta tatcttatta ctatctacat aaaaaaaatt ttattcacta ctatatttta
     2101 aaaccattaa ttaaaagaaa cccactctac ctcaacttcc attgactcat atttatttgt
     2161 agtataaaat tttatttcat cgctcttacc actattaaat cctatcaaaa cgcttggaaa
     2221 ttcttctata tttccatttt ctaatttgcc attaatgcta aaggctatat aatctaagtt
     2281 aattccactt gtattttcta caatcttgct atatttaaaa acctctttat cctcaccaaa
     2341 acctgatttt attgcttcat cctcttctaa ttcaacttta aactcaccct tagaaatcaa
     2401 ctcctttgca atcttcttat aatcctcttt tttatctaaa taagctacct tctctctaca
     2461 atcctcataa aagtccatat tctcttcatt tataaccaac ccatattcat caaccatctt
     2521 acatattgat atagatatga tttcatctga ctcactacca taatccataa cctccatata
     2581 gttttcacta cgtcccttgt aatattttgc tctaagttca tttcccttaa tataatcctt
     2641 aaaagtactt aataaagctt catcatatat tttttctact aaattctcta atcttttatt
     2701 ttccttctcc ataacacttg aaagttcaaa ttctatatcc tccaactttc cactagtatt
     2761 atcccttgat ataaccttaa tttcatttaa actctcattt ataatctctg atgctaaaga
     2821 taaagttctt ttatcagact ctcttttttc atcctcactt atcaatactt cctttaaatc
     2881 atctttttcc ttttcttttt ctgaagtatc ctctacattg tctcccttat gtttatctaa
     2941 actttcctta ttttcctcta cacaacctac aaagaataaa gttgaggcac aaactcctac
     3001 tattattcct tttttaaaaa tctctcctat taatgattta atattttttc tcataattta
     3061 atcctcaccc cttaagcttc ttaccataaa attttacctt ataatcaaat taaaatgatt
     3121 tatttccaaa gatttttaca cttaattaaa gcaaaattta taataaaaac tatcataaaa
     3181 ttaaatttta taaagtatat ctttaaaaat taaaaccaat taaaagagat tcttatgata
     3241 aaacatttaa taatgctatt aaaataaaaa aagaagtgac ttagactaaa cttaaatcac
     3301 ttcttttctt tataaactat attataaaat tcctattaaa ttaataaatt acttctaaag
     3361 cttgtccatc ccctcttata cctacttctt ctcataacaa tttctagaat tttcctaata
     3421 aaattattat gtcgagtaat aattttgtta tattaggcag ataatattaa gctacctaaa
     3481 aattaataac tttcataaaa ctattattta ttaactgaat aaaaacatct ctttggtgag
     3541 attatttttt cttctttttt taattctttt attgctttat caacatcttt tttatcaatt
     3601 ccagcttttt cagctatttc tccacctttt aaaggttctt ctgaactatt aagtacttct
     3661 aatacttttt ctttaatatc catgggaatc attcctttca ataaaatttg ttatacttac
     3721 attatatacc ataaaatttt atttttgaat agtaattatc aataaatata aattattaca
     3781 agttttttct tc
//
LOCUS       DAEVGI010000186         3764 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEVGI010000186 DAEVGI010000000
VERSION     DAEVGI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.188"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            113..1021
                     /locus_tag="K8M77_002320"
     CDS             113..1021
                     /locus_tag="K8M77_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBI7086774.1"
                     /translation="MDFVAIDFETANEKRNSACSIGLTVVKDNKIVEEKYFLIKPCEM
                     RFQPMNIWIHGIRPEDVENERTFNEIWKEIKDYIDGNLVIAHNAAFDISVLRKTLDFY
                     DIPYPDFEYACTVVMAKNYYKSLPNHKLGTVSEALGFEFSHHHAGEDARACANILLNI
                     CKDLDIESIEELSDKLAIRIGAVYENGYKSSGRKGECKYSSNLRLKEKKKEGEISLKC
                     KNVVFTGPLSSMSRGEAIGNVIRCGGFVSSSVSKKTNYLVTGIRDLSELKENEKSIKL
                     KKAENLIKENFDIKIINEEEFLSIIE"
     gene            1195..1539
                     /locus_tag="K8M77_002321"
     CDS             1195..1539
                     /locus_tag="K8M77_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086775.1"
                     /translation="MDKLFTISGVDFLAGDFQDDISEFQDIIPILQDFQKELKLEKIE
                     CTDENDCCFKTKENYICELVGALTEDDEFYSLKELRSDYNRFKDEMLYPFGIQVYKCT
                     ACNKWIINLLEE"
     gene            1550..2833
                     /locus_tag="K8M77_002322"
     CDS             1550..2833
                     /locus_tag="K8M77_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7086776.1"
                     /translation="MDKLRESITGVGHNICSFYKRVKDKIIKEKDPKVIIICVIALAL
                     IIVFLYGNVSSKKRDVIEQLSNSLSKGNERALMNLIEDGNKNSGMTKEELIPYIDFFK
                     ADKSRINKLVSSLENGDEIYSTKLESKKSFWGEKWYVVIQKKKLILVSNFPEANVYLN
                     GNFIGTTNISRTLEIPNLIPGVYDLKVEKKAYNSNLVEEKNIVFMDNNKIDIPLNGTL
                     VTVKSSFDDSNVYINGEDSGIKVKDFKDVGPFPKDGSSYLTIKCNTPWGEIYSKKFYI
                     EDHPEIKIELDLKDTIIKEDVEKVLKEFYSSVFQALNSEDKNDIRNAKDEVKDNIYNT
                     LSQKYFLLKNDYDISDLKIKMENSSIERNHGVYEASIVVNVSYKIKKKILGIDIKSES
                     MEKNFFTNMKYENGKWIVYSVQDFSLPGLEEEKSN"
     gene            complement(2882..3589)
                     /gene="deoD"
                     /locus_tag="K8M77_002323"
     CDS             complement(2882..3589)
                     /gene="deoD"
                     /locus_tag="K8M77_002323"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBI7086777.1"
                     /translation="MSIHIGAKEGQIAETILLPGDPLRAKFIAETFLENPVCYNEVRG
                     MLGYTGTYKGKRISVQGTGMGVPSISIYVNELIRDYGVKNLIRVGTAGGMQEDIKVRD
                     LVLAMSSHTDSAINKVRFNGLDFAPTASFKLLKAAYDTAVEKGYSPKVGSVFTADSFY
                     NDNPEAWKQWAKFGTLAVEMETAALYTLAAKYGVNALTILTISDHLITAEETTSEERQ
                     TTFTKMMEVALDAAITL"
BASE COUNT         1507 a          374 c          629 g         1254 t
ORIGIN      
        1 atttctacag taaggtcttt ttattttgaa ttacatttaa ataaattaaa acaataaaat
       61 aaaatgtgat ataatattgt ttattgttac atagaaaggg agaaagacat aaatggattt
      121 tgttgcaata gactttgaaa ctgctaatga gaaaagaaat agtgcgtgtt ctattggatt
      181 gactgttgtt aaggataata aaattgttga agaaaaatat tttttaataa agccttgtga
      241 aatgagattt cagcccatga atatatggat acatggaatt agaccagagg atgttgaaaa
      301 tgagagaact tttaatgaaa tatggaaaga aataaaggat tacatagatg gaaatttagt
      361 aatagctcat aatgcagctt ttgatattag cgttcttaga aaaactttag atttttatga
      421 tataccttat ccagactttg agtatgcttg tacagtagtt atggcaaaga attattataa
      481 atcattacca aatcataaat taggaactgt aagtgaagct ttaggctttg aattttctca
      541 tcaccatgca ggggaagatg caagggcttg tgctaacata ttacttaata tatgcaaaga
      601 tttagatata gaatcaattg aagagctttc agataagctt gccataagaa ttggtgctgt
      661 ttatgaaaat ggatataaat cttctggaag aaagggagaa tgcaaatatt caagtaattt
      721 aagacttaag gaaaagaaga aagagggaga aatatcttta aaatgtaaaa atgtagtttt
      781 tacaggacct ttaagttcta tgtctagagg agaagctata ggaaatgtaa taagatgtgg
      841 tggatttgtt tcaagttctg taagtaaaaa gacaaactac ttagttacag gaattaggga
      901 tttaagtgaa ttaaaggaaa atgaaaagag tattaaatta aaaaaagcag aaaatttaat
      961 aaaagaaaat tttgatatta agataataaa tgaagaggag tttttatcta taatagaata
     1021 aataaacttt aattttagat ataaatagct catttaaggg atatttttac tattgttaac
     1081 aataagttat ttacaataat atttattgtg ctaaaataag ttagtaaaat taattaattt
     1141 atgcagtact agaattagtt agcaaatttt aattaggaaa ttagaggtga ttaaatggat
     1201 aaattattta ctataagtgg agtagatttt ttagcagggg attttcaaga tgacataagt
     1261 gaatttcaag atataatacc tatattacaa gattttcaaa aagaattaaa gttagagaaa
     1321 atagagtgta cagatgaaaa tgattgctgt tttaaaacta aagaaaatta tatatgcgaa
     1381 ttagtaggag ctttaactga agatgatgaa ttttattcat taaaagagtt aagaagtgat
     1441 tataatagat ttaaagatga aatgctatat ccttttggaa tacaagttta taaatgtaca
     1501 gcatgtaata aatggatcat aaatttactg gaggagtaag ttttaacata tggataaatt
     1561 gagagagagt attacagggg taggtcataa catttgtagc ttttataaaa gagtaaagga
     1621 taaaattata aaagaaaagg atcctaaggt aataataatt tgtgttattg ctttagcctt
     1681 aatcatagta tttctatatg gtaatgttag tagtaaaaag agagatgtaa ttgaacaatt
     1741 atctaattcc ttaagcaaag gaaatgaaag agctttaatg aatttaatag aagatggaaa
     1801 taaaaattct ggaatgacaa aagaggagtt aataccatat attgattttt ttaaagcgga
     1861 taaaagtaga attaataagt tagtttcttc tttagaaaat ggggatgaga tatactctac
     1921 aaaattggag tctaaaaaaa gtttttgggg agaaaaatgg tatgttgtaa tacaaaaaaa
     1981 gaaacttatt ttggtatcta atttcccaga agctaatgtg tatttaaatg gtaattttat
     2041 aggtacaact aatattagta gaactttaga aatacctaat ttaataccag gagtatatga
     2101 tcttaaggtt gaaaagaaag catataattc taatttagta gaagaaaaaa atattgtttt
     2161 tatggataac aataagatag acataccttt aaatggaacc ttagtcacag ttaagagtag
     2221 ttttgatgat agtaatgtat atataaatgg agaggactca ggtataaagg taaaagattt
     2281 taaggatgtt ggtcctttcc caaaggatgg ttcatcttac ttaactataa aatgcaacac
     2341 tccttgggga gaaatatata gtaaaaagtt ttatatagaa gatcatccgg aaataaagat
     2401 agaattagat ttaaaagata caattataaa agaagatgtt gaaaaagttt taaaagaatt
     2461 ttatagcagt gtatttcagg ctttaaattc agaggataaa aatgatataa gaaatgctaa
     2521 ggatgaagtt aaggacaata tatataatac attaagtcaa aaatattttc ttttaaagaa
     2581 tgactatgat ataagcgatc ttaaaattaa aatggaaaat agctcaatag aacgaaatca
     2641 tggtgtttat gaagctagta ttgtagttaa tgtttcttat aagataaaaa agaaaatatt
     2701 aggaatagat attaaaagcg aatcaatgga aaaaaacttt ttcacaaata tgaagtatga
     2761 aaatggaaaa tggatagttt atagtgtaca agacttttca ttgccaggat tagaagaaga
     2821 gaagagtaat taataaataa aggagcttta tcaaaattca ttttgataaa gctcctttat
     2881 tttataaagt tatagcagca tcaagagcaa cttccatcat ctttgtgaag gtagtttgac
     2941 gctcctcact agtagtttcc tcagcagtaa ttaaatgatc acttatagta agtattgtta
     3001 aggcattaac accatactta gctgctaagg tatataaagc tgcagtttcc atttcaacag
     3061 ctaaagtacc aaatttagcc cattgtttcc atgcttcagg attatcatta tagaagctat
     3121 cagctgtgaa tacgctacca accttaggag aatatccttt ttcaacggca gtatcgtaag
     3181 cggcctttaa aagtttaaat gatgctgttg gagcaaaatc taaaccgtta aatctaactt
     3241 tatttattgc agaatcagta tgagatgaca ttgctaaaac taaatctcta acttttatat
     3301 cttcttgcat acctccagca gtacctactc ttattaaatt tttaactcca taatctctta
     3361 taagttcatt aacgtaaata gaaattgaag gaacccccat accagtacct tgaactgata
     3421 ttctttttcc tttatatgtt cctgtatatc ctaacattcc tctaacttca ttgtaacaaa
     3481 ctggattttc taaaaaagtt tcagctataa atttagctct taaaggatct cctggtaata
     3541 aaattgtttc ggctatttga ccttcttttg cacctatatg aatactcata atgtacctcc
     3601 taataatgta aatgttttat aaataaattt tatcataaat aatgaaaaaa tactaaaaat
     3661 tataaaaaat taatttaagt agttaaagtt tatttagtat gttatattaa tagataataa
     3721 taaataataa taatttatta aatttgtatt tataaaaata atga
//
LOCUS       DAEVGI010000187         3763 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEVGI010000187 DAEVGI010000000
VERSION     DAEVGI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.189"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(95..1501)
                     /locus_tag="K8M77_002324"
     CDS             complement(95..1501)
                     /locus_tag="K8M77_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI7086778.1"
                     /translation="MKDVKVLKKEYENAWTKYEEADLKQVFSLSDRYREFMSVAKTER
                     ECVKVLANMAESKGFKNFYEVIKNGEKVTAGDKLYSINMDKTITLIKVGSEPLENGLR
                     IIGAHIDSPRIDVKQNPLYEDSGLALLDTHYYGGVKKYQWVTIPLAIHGVVVKKDGTR
                     VDIKIGEDENDPVLGISDLLIHLSADQLDKKGAKVVEGEDLNILVGSMPLKGTEEKEA
                     VKANILVLLNEKYGITEEDFVSAELEVVPAGKARDYGLDRSMILAYGHDDRICAYTSA
                     EALMDLENVDKTCVALLVDKEEIGSVGATGMQSRFFENIIAELMDRKGEYSELKLRRC
                     LQNSMMLSADVTAAFDPNYPSVCEKKNTAYFGHGVVFSKYTGARGKAGCNDANAEYIA
                     HLRNIMDKNGVVWQTGELGKVDQGGGGTIAYILAQYNMEVIDCGVALQNMHAPLEVAS
                     KADLYETKKCYKAFFEEA"
     gene            complement(1568..2497)
                     /locus_tag="K8M77_002325"
     CDS             complement(1568..2497)
                     /locus_tag="K8M77_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBI7086779.1"
                     /translation="MVIFNIEENFKVVEEWKKDEFPAWILMEEDEVNLLEKMFSYECI
                     NECIDYKQEQRIDCFKDYTFILINIIEQVEKQIISKELDIFLGKSFIVTVYKSNISLL
                     YDLIEDIKEEKNCQLFKVDKSPSIILYYILDRIIINNYNIISDLETKGDKIELEILKD
                     PESKHLNSLLFIRREVYKLRKLIKPIRYIGDTLVIDENEVIGEGNKVYFKNINSKIEK
                     LITSLDTLSQELAIVREAYESELANKTNELMKVFTIITAVFLPLELITAIFSMSFESM
                     PFKTCPYGFYILVTILSLMALILLLIFRIRKFL"
     gene            complement(2542..3465)
                     /locus_tag="K8M77_002326"
     CDS             complement(2542..3465)
                     /locus_tag="K8M77_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBI7086780.1"
                     /translation="MGVTIEKLIKDFSLEVIQTGEENVPINVSDVNRPGLQLAGFYNY
                     FAPERIQVIGKAEWSFLEDMSPDLRKKRLNKFFSFDISCLIITRGLEIHEELLKAARK
                     RNLWILRSDMVTTKFISKITMYLSDKMAPETRLHGVLVDVYGIGMLITGESGIGKSET
                     ALELIKRGHRLVTDDAVDIKEIDGDLIGRSPEITFGMLEVRGMGIIDVSALYGLSSIL
                     NSKQIKIIIHFEHWKDDGDYDRLGVNDEYQDILGVKVKKLRVPIRPGRNIAVIIEAAA
                     ANYRYQRMSDISPVDIIEKRMLESMEKESKI"
     gene            complement(3477..3656)
                     /locus_tag="K8M77_002327"
     CDS             complement(3477..3656)
                     /locus_tag="K8M77_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBI7086781.1"
                     /translation="MNIDELTKRINELHKKHKEEGLSEDEHKEREELRKEYINRFKSN
                     LREQLKGIEPKNKKN"
BASE COUNT         1151 a          711 c          345 g         1556 t
ORIGIN      
        1 ttatataaaa agactatatc aaaatttatc taatatacta taaatcatat atattagatt
       61 aaatattttg atatagcctt ctttaactag ttaattatgc ttcttcaaaa aatgccttat
      121 aacatttttt agtttcatat aaatctgctt tagatgctac ttctaaaggc gcatgcatat
      181 tttgtaatgc tactccacaa tctataactt ccatattgta ttgagctaat atataagcga
      241 ttgtaccacc gccaccttgg tctacttttc caagctctcc agtttgccat acaacaccat
      301 ttttatccat tatatttctt aagtgagcta tatattctgc attagcatca ttgcaacctg
      361 cttttcctct agctcctgta tatttgctaa atactactcc atgtccaaaa taagctgtgt
      421 ttttcttttc acatacagaa ggatagtttg gatcaaaagc tgcagtaaca tcagctgata
      481 acatcattga attttgaaga catcttctaa gctttaactc agaatattct ccttttctat
      541 ccataagttc tgcaattata ttttcaaaaa atcttgattg cattcctgta gctcccacac
      601 tacctatttc ttctttatca actaataatg ctacacatgt tttatcaaca ttttctaaat
      661 ccattaaagc ttctgctgaa gtatatgcac atattctatc atcgtgacca tatgctaaaa
      721 tcatacttct atctaatcca taatctcttg ccttaccagc aggaactact tctaactcag
      781 ctgatacaaa atcttcctca gttatgccat atttttcatt taataaaact aatatattag
      841 ctttaacagc ctctttttcc tcagttcctt ttaatggcat acttccaact aaaatattta
      901 ggtcttctcc ttctactact ttagctcctt ttttatctaa ttgatctgct gataaatgga
      961 ttaaaagatc tgaaattcct aaaacaggat cattttcatc ttctcctatt ttaatatcta
     1021 ccctagttcc atcttttttt acaacaactc catgtattgc aagaggtata gttacccatt
     1081 gatatttttt tactcctccg taataatgag tatctaataa tgctaatcct gaatcttcat
     1141 ataatggatt ttgtttaaca tctattcttg gagaatctat gtgagctcct attattctta
     1201 atccattttc taatggttct gaacctactt ttattaaagt tattgtttta tccatgttta
     1261 tagaataaag tttatcccca gctgtcactt tttctccatt tttaataact tcataaaaat
     1321 ttttaaaccc tttactttct gccatattag ctaaaacctt aacacattct ctttcagttt
     1381 tagcaactga catgaactcc ctatatctat cacttaatga aaatacctgt tttaaatctg
     1441 cctcttcata tttagtccaa gcattttcat attctttttt taaaactttt acatctttca
     1501 taatatatcc ctccaatcta ttttatcttc taattcaatt ttatattttt ggtaatattt
     1561 tgtcaaacta tagaaatttt cttattctaa atattaatag caaaattaga gccattaaac
     1621 tcagtatagt aactaaaata taaaatccat aaggacatgt tttaaaaggc atagattcaa
     1681 agctcataga aaatatagct gttattaatt ctaaaggtaa aaaaactgct gttattattg
     1741 taaatacctt cattaattca tttgtcttgt tggctagttc tgattcataa gcttctctta
     1801 caattgctaa ctcctgtgat aaggtatcta aagatgtaat taacttttct attttactat
     1861 ttatgttttt aaaataaact ttattccctt ctcctattac ttcattttca tctataacaa
     1921 gggtatctcc aatatatcta attggcttta taagttttct aagtttataa acttctcttc
     1981 ttataaataa tagagaattt aaatgcttgc tttcaggatc tttaaggatt tctaactcaa
     2041 tcttatctcc tttagtctct aaatcagata ttatattata gttatttatt ataatcctat
     2101 ctaaaatgta ataaagaata atagaggggc tcttatctac tttaaataat tgacagttct
     2161 tttcttcctt tatgtcttca attaaatcat ataaaagtga gatattactc ttataaacag
     2221 taactataaa actttttcct aagaatatat caagttcttt agagataatt tgtttttcaa
     2281 cttgctctat tatattaatt aaaataaatg tataatcctt gaaacagtct attctttgtt
     2341 cctgtttata gtcaatacat tcatttatac actcataaga aaacattttt tctaaaagat
     2401 tgacttcatc ttcttccatt aaaatccatg ctggaaactc atcttttttc cactcttcaa
     2461 ctactttaaa attttcctca atattaaata taaccatatc atcaccttta actctgatat
     2521 ggttatagtt tatacttttt attaaatttt actctctttt tccatacttt caagcattct
     2581 tttttcaatt atatcaactg gtgaaatgtc tgacatacgt tgatatctat aattagctgc
     2641 agcagcttct attattactg ctatgtttct tccaggtctt attggaaccc ttagtttttt
     2701 aactttaaca cctaatatat cttgatattc gtcgtttaca cctaatctat cataatctcc
     2761 atcatctttc caatgctcaa aatgaattat tatttttatt tgcttagaat ttaaaattga
     2821 acttaatcca tataatgctg atacatcgat tattcccata cctctaactt caagcattcc
     2881 aaaagttatc tctggcgatc tgcctattaa atctccatct atttctttta tatcaacagc
     2941 atcatctgtt actaatctat gtcctctttt aataagttct agggcagttt cacttttacc
     3001 tataccactt tcccctgtga ttaacattcc tataccatat acatcaacaa gaaccccatg
     3061 taatcttgtt tcaggagcca ttttatctga taaatacata gttattttac ttataaactt
     3121 agtagttacc atgtcacttc taagaatcca taagtttctc tttctagctg cttttaaaag
     3181 ttcctcatgt atctctaaac ctctagttat tataagacaa gatatatcaa aggaaaaaaa
     3241 tttatttaat cttttcttcc taagatctgg agacatatct tctaaaaagc tccattcagc
     3301 ttttcctata acttgaattc tttctggagc aaaatagtta taaaagccag ctaattgtaa
     3361 tcctggtcta tttacatcac taacatttat agggacattc tcttcgccag tttgaattac
     3421 ctctaaacta aaatccttta ttaacttctc tattgtaaca cccacataaa tcacccttaa
     3481 tttttcttat ttttcggctc aattcctttt aactgttctc ttaaattgct tttgaatcta
     3541 tttatatatt cttttctaag ttcttctctt tctttatgtt catcctctga taaaccttct
     3601 tctttgtgct ttttgtgtaa ttcatttatt ctttttgtta attcatctat attcatgttt
     3661 attctccttc ttagtataat tttaatttga tttaatttat ttctaatatt attttacatt
     3721 tgtatctatt ttttagcaac aatctttctt actaaattct tca
//
LOCUS       DAEVGI010000188         3758 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEVGI010000188 DAEVGI010000000
VERSION     DAEVGI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.190"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            155..1066
                     /locus_tag="K8M77_002328"
     CDS             155..1066
                     /locus_tag="K8M77_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBI7086782.1"
                     /translation="MDKITLVIMAAGMGSRYGGLKQIDKIGPKGKTIIEYSIYDAIKA
                     GVTKVVFIIKKDIFKKFRETIGIKIENIIETKYVFQEVDNLEKEFKGNFKRVKPWGTG
                     HAILCCKGFIKEPFIVINADDFYGRETYKVLCEEIKKSKCDSEYFMVGFRVGKTLTDF
                     GSVSRGICDLNSKGELISIKEKTKIMKKGNSICYEENGNYYNISKNTLVSMNIWGLKP
                     SIFTELEYRFLNFLKDKSIDKCKEEFYLPTAIDELIKEDRVNVKVLKTNEQWYGVTYK
                     EDKESVRDSLKNLSKKIYPDDLWEEFK"
     gene            1068..2174
                     /locus_tag="K8M77_002329"
                     /pseudo
     CDS             1068..2174
                     /locus_tag="K8M77_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462478.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
     gene            2335..3729
                     /locus_tag="K8M77_002330"
     CDS             2335..3729
                     /locus_tag="K8M77_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBI7086783.1"
                     /translation="MVNIMSKKETDLGSDSIGKLLFKLAMPAIIAQLVNVLYNIVDRV
                     FIGRLQSGDMAMAGVGVAFPIIMIVAAFSGLVGMGGAPLAAIKMGEKKKEEAEEIMTN
                     SFSALIGLALFLTVFFFIFKNPILWAFGASDATIGYALDYLGIYLIGTIFVQIALGMN
                     SFINTQGFAKVGMITVMVGAIINIVLDPILIFGFNMGVKGAALATIIGQCASAIWVLY
                     FLFGKQSILRIRKKFLKPKLKILFSIMALGVSPFIMQATESLVIISMNNNLLKYGGDL
                     AVGAMTIMSSVMQIILLPLMGLSQGAQPIISYNYGANKVYRVKKAFKLLLVSSMVFTT
                     IMWSALMILPDVFVKIFNSNNPQLMEITSWSIKIYFAGILLFGAQVACQQTFLALGQA
                     KISLILALLRKIVLLIPFIYALPMIFGNKLMGVLIAEPFADIIAALSTVTCFIVFYKK
                     HLSQGLEEEIKVNS"
BASE COUNT         1478 a          358 c          686 g         1236 t
ORIGIN      
        1 aattatttca atatttaaca atattttaat tttttataaa atagagagaa aatttacatt
       61 gtatttagta aaatgaattt aactatgtta aataacttat aaaatttatt tatgttttaa
      121 ggggagaaaa gtattaaaaa gtgaggggat gaaattggac aagattactt tagtaataat
      181 ggcagcagga atgggaagta gatatggagg gttaaagcaa attgataaaa tagggccaaa
      241 gggaaagacc ataatagaat attccattta tgatgctatt aaagctggtg ttacaaaggt
      301 tgtatttata ataaaaaaag atatttttaa gaaatttaga gaaactatag ggattaaaat
      361 agaaaatata atagagacta aatatgtttt tcaagaagtt gataatttag aaaaagaatt
      421 taaaggaaat tttaaaagag ttaaaccctg gggaactgga catgcaattt tatgttgtaa
      481 aggttttata aaagaacctt tcatagttat aaatgctgac gatttttatg gaagagaaac
      541 ttataaagta ctttgtgaag aaattaaaaa aagtaagtgt gattctgaat actttatggt
      601 ggggtttagg gtaggaaaaa cattaactga ctttggaagt gtatctagag gaatatgtga
      661 tttgaattct aagggagaat taatttctat taaagaaaaa acaaaaatta tgaaaaaagg
      721 aaatagtata tgctatgaag agaatggaaa ttattataat atttctaaaa acaccttagt
      781 ttctatgaat atttggggat taaaacctag catatttaca gaactagaat atagattttt
      841 aaatttttta aaagataaaa gtatagataa atgtaaagag gagttttatt tgcctacagc
      901 aatagatgag cttataaaag aagatagggt taatgttaag gttttaaaaa caaatgaaca
      961 atggtatgga gttacttata aagaggataa agaaagcgtt agggattctt taaaaaattt
     1021 atctaaaaaa atttatccag atgatttatg ggaggagttt aaataaaatg aacaagactt
     1081 atgattttaa aagcatagca gaaaagttta atttagaggg agagctaaaa acatcagata
     1141 gccatgttta tggacatata aatgatacat ttataataaa ttgtgaaggg aaaaatggag
     1201 aagaaataaa ataggttctt caaagagtaa actctgatat attcaaaaat ccagagcaac
     1261 taatggagaa tattgaaaat gttacttcac atataaaaaa taaaattata gaagaaaatg
     1321 gagacccttt aagggaaact ttaaatatag tgaagacaaa agaaggtaaa agtttctata
     1381 aatgcaaaga gggaaattat tggagaattt ttaatttcat ccatggtgct agcacatatc
     1441 aaatagttga aaagcctgag catttatata cagctggtaa agctttagga aaatttcaaa
     1501 agcagttatc agattttaat gttgatatgc tttatgatac aatccctgat tttcataata
     1561 cagaaaagag atttgaggca tttatggaat ctgtaaggga agataaaaaa gggaaagcaa
     1621 aggatgtaaa agaagaaata gattttgtaa ttaaacgtgc tgaagataca aaagttttag
     1681 taaatatgat aaaagaaaat aaacttcctt taagagttac tcataatgat acaaagttta
     1741 ataatataat gatagatgat gaaacaggag aaggaattgc agttattgat ttagatactg
     1801 ttatgccagg actatcacta tatgactttg gagattctat tcgttctgga gctacaactg
     1861 ctttagaaga tgaagtggat ttatctaagg taaactttga tctaaatctt tatgagcatt
     1921 ttgcaaaagg atttttagag tcagcaggag atgcctttac aaaagaggaa attgaatatt
     1981 taccttttgc agctaaactt atgacctttg aatgtggaat gagatttctt atggattatt
     2041 taaatggaga tgtttacttt aaaatacata gggaaaccca caacttagat agagctagaa
     2101 atcaatttaa gcttgtttct gatatggaaa aagaaatgga caatatgaag aagatagtat
     2161 attcatacat ttaaaaactc tattttttat gaaaatatat ttggcattct ttaaaacatt
     2221 ataaatttag ataatcctaa tatggaattt agacactatt taaaatgtac aagtaataaa
     2281 tattattgta tgaattatat aaaaaatcat gagtataaat aaattaggga aagaatggtg
     2341 aatattatga gcaaaaaaga aacagattta ggaagtgata gtattggaaa gctccttttt
     2401 aaactagcta tgccagctat aatagctcaa cttgttaatg ttttatacaa tatagtagat
     2461 agagtgttta taggaagatt gcaatctggt gatatggcta tggctggagt tggggtagcc
     2521 tttccaatta taatgatagt cgctgctttt agtggtttag ttggtatggg aggagctcct
     2581 ttagcagcga ttaaaatggg agaaaagaaa aaagaagagg ctgaagaaat aatgacaaat
     2641 agtttttctg cacttatagg attagcttta tttttaacag tatttttctt tatatttaag
     2701 aatccaattt tatgggcctt tggggctagt gatgcaacta taggatatgc tttagattat
     2761 ttaggtattt atttaatagg tactatattt gttcaaattg ccttaggaat gaattccttt
     2821 ataaatacgc aaggatttgc taaggttgga atgataacag ttatggtagg agctataata
     2881 aatattgtat tagatcctat tttaatcttt ggatttaata tgggagttaa gggagcagct
     2941 ttagcaacta taattggtca atgcgcctct gctatatggg tattatactt tttatttgga
     3001 aaacaaagta ttcttagaat aagaaaaaaa ttcttaaagc caaaattaaa aatattattt
     3061 tcaataatgg ctttaggagt atctccattt ataatgcaag ctactgaaag tttagtaata
     3121 ataagtatga ataataattt attaaagtat ggtggagact tagccgttgg agcaatgact
     3181 attatgagta gtgtaatgca aataatatta cttccattaa tgggactttc tcaaggggca
     3241 caacctataa taagttataa ctatggggct aataaagtct atagagtaaa aaaagccttt
     3301 aaattacttt tggtatcatc aatggtattt accacaatta tgtggagtgc attaatgatt
     3361 ttaccagatg tgtttgtaaa gatttttaat agcaataatc cccaattaat ggagataact
     3421 tcatggagta ttaaaatata ctttgcaggt attctgcttt tcggtgcaca agttgcttgt
     3481 caacaaactt ttttagcttt aggacaagca aaaatatcat taattttagc cttacttaga
     3541 aaaattgtgc ttttaatacc atttatatat gcgcttccaa tgatttttgg aaataagctt
     3601 atgggagttc ttattgcaga accttttgca gatataatag cagctctatc aacagtaaca
     3661 tgctttattg tattttataa aaaacattta tcacaaggtt tagaagaaga gataaaagta
     3721 aattcttaat aaaaacaaaa aagtctagag aaatttaa
//
LOCUS       DAEVGI010000189         3755 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEVGI010000189 DAEVGI010000000
VERSION     DAEVGI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.191"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            22..3630
                     /locus_tag="K8M77_002331"
     CDS             22..3630
                     /locus_tag="K8M77_002331"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase/5'-nucleotidase"
                     /protein_id="HBI7086784.1"
                     /translation="MLKKFSNKKITALMVVLAMVFSFILPIQVKAAEISEAKNSNVTE
                     KTDKKTVNIQILATSDLHGKFMDYDYAQGEESVGGLNQIATVVKEAKKENPNTLVLDN
                     GDTIQGNYNHLFMNKENPMILAMNTIGYDVFSLGNHEFNFGMDKLHNIIGQANQNLHV
                     LCGNLYRNGKRVFNPYTIKEVDGIKVAIIGVVTPHIMQWDSQNLKGYEAKNPTEEVKK
                     IIGEIKANGGADVYVVTSHASLNGEYGDGDSAAGIAEANPEVSVVVAGHSHDTVKSEL
                     RGNSIISEPASRAKYVSKFNLTVEKNESGIKVLDKSADLISLKGVQGDPELTEKLKPF
                     HEAAINDATAKIGELKGGDLAKPDEVKGIPQSIVEDQGVTDFINEVQLYNSKKFLQTK
                     GIDPNNVYMVSSAALFSPKANLKEGPISKADVSNIYKFDNKLYVIKTNGKQLKKYMEE
                     NSKFFNKYKDGDLTISFDENVRMYKYDMFEGVNYEINIAKDPGERIENLKFSKDGKPV
                     EDSDVVYLSVNDYRYNSGLAAGIMDSGEHEKIYDTVNDDISAIRDLISDYIINVKHGV
                     INRNVDGNWKITGNNWNPEQRALAVKLINEGKIKLPTSSNGRTPNVKSVTWDEVSKFA
                     EALPEEKKEVEIPILTFNDFHGSLKESGGNPGAAKFVGELKKVKEKNPNTIVVSGGDM
                     YQGSALSNLLKGKPVSDMNKALGVQFSSVGNHEFDWGYDLIPGWAKDGGFEFLASNIY
                     EKATGEPVKWAKPYGVVEKGGKKIGFIGLATPETAYKTNPDNVKDLEFRDPNTEAEKW
                     VKHLREVEKVDAVIALTHIGSQQDRETGEITGEVVNLTKVPGLDAIVSAHSHMPVKGT
                     VNGVPIVQAYKNGRAIGLIDLKFDKDGKLSVEAQRMDISKNIKDLPVDKEMEETLGKY
                     EKELSPILDVKVADLSEDLPHNRDVGVSPMGATVTETMRKIVDADIAINNGGGVRAPL
                     KKGVITVGDMYTILPFDNTIVTMDMKGSDIIKVLEHGIAPKDFGWGQHAGLKFWYNPD
                     AEPGNRITSVRLLDGSKLDPNKYYKLATNDFMAAGGDGYDFSAAKNIKDTNKVLREGI
                     SDYWKNHGISPITNLATLIEPGEDKSEAIKPVEQEKPSIDTNKPMDPTIDNINTDNPE
                     ISNPDNNSNNEENKKDDLIKLPNTGNPFGAEVFAAMGVLATIGGSIILKKKHNDAA"
BASE COUNT         1528 a          423 c          723 g         1081 t
ORIGIN      
        1 aaaattcaag ggggataatt tatgttaaag aagtttagta acaaaaaaat tactgcttta
       61 atggttgtat tagcaatggt ctttagcttt atactaccaa tacaagttaa agctgctgaa
      121 atttctgaag caaaaaattc aaatgtaaca gaaaagacag ataaaaaaac tgtcaatatt
      181 caaatacttg ccacttccga tttgcatggt aagtttatgg attatgatta tgcacaggga
      241 gaagaatctg ttggtggatt aaatcaaata gcgactgtgg ttaaagaagc taaaaaggaa
      301 aatcctaata ctctagtact agataatggt gatactattc aaggaaatta caatcaccta
      361 tttatgaata aagaaaatcc tatgatactt gctatgaaca caataggata tgatgttttt
      421 tcattaggaa atcatgaatt taattttgga atggacaaac ttcataatat tattggtcaa
      481 gcaaaccaaa atcttcatgt cttatgtgga aacctttaca gaaatggaaa gagagttttt
      541 aatccttata caattaaaga ggtagatgga ataaaggttg caataatagg agtagttact
      601 ccacatataa tgcaatggga ttctcaaaac ctaaaaggtt atgaagctaa gaatccaaca
      661 gaagaagtaa agaaaataat aggagaaata aaggcaaatg gaggcgctga tgtctatgta
      721 gtaacttctc atgcaagttt aaatggagaa tatggtgatg gagatagtgc tgctggaata
      781 gctgaagcaa atccagaagt aagtgtagta gttgctggac atagtcatga tacagttaaa
      841 tcagaattaa gaggaaattc tataattagt gaaccagcaa gtagagcaaa atatgtttct
      901 aaatttaatt taactgttga aaaaaatgaa agtggaataa aagttcttga taagagtgca
      961 gatcttataa gtcttaaagg ggtacaagga gatccagaat taactgagaa acttaaacct
     1021 ttccatgaag ctgctataaa tgatgcaaca gctaagattg gagaattaaa aggtggagat
     1081 ttagcaaaac ctgatgaagt aaaagggatt ccacaatcaa tagttgaaga ccaaggagtt
     1141 actgatttta taaatgaagt acaattatat aatagtaaaa agtttttaca aactaaagga
     1201 atagatccaa ataatgtgta catggtttca tcagcagctt tatttagtcc taaggctaat
     1261 cttaaagaag gtcctataag caaggctgat gtttcaaata tatataaatt tgataataaa
     1321 ttatatgtta taaaaactaa tggaaaacaa ttaaagaaat acatggaaga aaactctaag
     1381 ttctttaata aatataaaga tggagattta actatatctt ttgatgaaaa tgtaagaatg
     1441 tataagtatg atatgtttga aggggttaat tatgaaataa atatagctaa agatccaggt
     1501 gaaagaatag aaaacttaaa attctctaaa gatggtaaac cagttgagga ttcagatgta
     1561 gtgtatttat cagtaaatga ttatagatat aattcagggt tagctgcagg aataatggat
     1621 tcaggtgaac atgaaaaaat atatgataca gtaaatgatg atatatcagc aataagagat
     1681 ttaataagtg attatataat taatgttaaa catggagtaa taaatagaaa tgtagatgga
     1741 aactggaaaa taacaggtaa caattggaat ccagaacaaa gagctttagc agttaaactt
     1801 attaatgaag gaaaaataaa gctaccaact tcatcaaatg gaagaactcc aaatgttaaa
     1861 tctgttactt gggatgaagt atcaaaattt gcagaggctt taccagaaga aaagaaagaa
     1921 gttgaaattc ctattttaac attcaatgat tttcatggtt ctttaaaaga atcaggagga
     1981 aatcctggag ctgctaaatt tgttggggaa ttaaagaaag taaaagaaaa aaatcctaac
     2041 acaatagtag taagtggtgg agatatgtat caaggaagtg cattatctaa ccttttaaaa
     2101 ggaaagccag ttagtgatat gaataaagct ttaggtgttc aattttcatc tgtaggaaat
     2161 catgaatttg actggggtta tgatttaatt ccaggatggg ctaaagatgg tggatttgaa
     2221 ttcttagctt ctaatatata tgaaaaagct acaggagaac ctgtaaaatg ggctaaacct
     2281 tatggagttg ttgagaaagg tggaaagaaa ataggattta taggccttgc aacacctgaa
     2341 acagcatata aaacaaatcc tgataatgta aaagatttag aatttagaga ccctaacact
     2401 gaagcggaaa aatgggttaa acatttaaga gaagttgaaa aagttgatgc agtaattgct
     2461 ttaacacaca ttggaagtca acaagataga gaaactggag aaataacagg tgaagtagtt
     2521 aatcttacaa aggttccagg cttagatgca attgtatcag ctcatagtca tatgcctgtt
     2581 aaaggaactg ttaacggagt tccaatagta caagcttata aaaatggacg tgctattggt
     2641 cttatagatt taaaatttga taaagatgga aaattatctg ttgaagctca acgtatggat
     2701 atttctaaaa atattaaaga tttacctgta gataaagaaa tggaagaaac attaggaaaa
     2761 tatgaaaaag aattatcacc tatcttagat gttaaggtgg ctgatttaag tgaggatctt
     2821 ccacacaata gagatgtagg ggtaagccca atgggagcaa ctgttacaga aactatgaga
     2881 aaaatagtag atgcagatat agctataaat aatggtggtg gagtaagagc tccattaaag
     2941 aaaggggtaa taacagtagg agatatgtac actatacttc cttttgataa tactatagta
     3001 acaatggata tgaaaggttc agatattatt aaagtattag aacatggaat tgctcctaaa
     3061 gattttggat ggggccaaca tgctggtctt aaattctggt ataatccaga tgctgaacca
     3121 ggaaatagaa taacttctgt aagattatta gatggaagta agctagatcc aaataaatat
     3181 tataaattag cgactaatga ttttatggca gctggtggag atggatatga tttctcagca
     3241 gctaagaata ttaaagatac taacaaagtt ttaagagaag gtataagtga ttattggaaa
     3301 aatcatggta taagtcctat aactaactta gcaactctta ttgaacctgg agaagataaa
     3361 agtgaagcta ttaaaccagt tgaacaagaa aaaccatcta tagatacaaa taagccaatg
     3421 gatccaacta tagataatat taatacagat aaccctgaaa tatctaatcc agataataat
     3481 tcaaataatg aagaaaataa aaaggatgat ttaattaaat tacctaacac aggaaatcct
     3541 tttggagctg aagtttttgc agcaatggga gttttagcta ctataggcgg tagcataatt
     3601 ctaaaaaaga aacataatga tgcagcttaa tttaaaatga ctaaaaagca gtaaagaaat
     3661 tttctttact gcttttatta tttaagaaga gtaattgatt attaaattta tttaagcata
     3721 gaatttacta ggaattaaaa aataaaaaag ttaac
//
LOCUS       DAEVGI010000190         3735 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEVGI010000190 DAEVGI010000000
VERSION     DAEVGI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.192"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..532
                     /locus_tag="K8M77_002332"
     CDS             <1..532
                     /locus_tag="K8M77_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HBI7086785.1"
                     /translation="KALKITLTEKNIIYSTRDIPYGIEDYNRGFSIKLSKVRRNSTSK
                     LTYLKSTCYIENIMEKEKAKDEGFDEVIFLNERGELTEGATSNIYFIKNNKICTPKVS
                     CGLLDGIIRGWIKNNFQVEEGCYNIEDLRNSEGIFISNSLLGIMRVSQFEEEFFGEKD
                     LIEEIKSKYESSIISF"
     gene            547..1104
                     /gene="folE"
                     /locus_tag="K8M77_002333"
     CDS             547..1104
                     /gene="folE"
                     /locus_tag="K8M77_002333"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBI7086786.1"
                     /translation="MIDKDKIREGMKLILEAIGEDANREGLIETPDRIARMYEEIFSG
                     IGMNAKEHLSKTFEVHSNDLVLEKDITFYSMCEHHLVPFYGKVHIAYIPNGRVVGLSK
                     LARCVEVYSKKPQLQERLTTEIADSIMEYLDAQGVMVVVEGEHMCMTMRGVRKPGAKT
                     VTTTYRGKFLEDESLKNDVFRMISM"
     gene            1129..1602
                     /locus_tag="K8M77_002334"
     CDS             1129..1602
                     /locus_tag="K8M77_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBI7086787.1"
                     /translation="MERINKILNNSKYKDYLNRNSFCEKDRVFCKHNLEHFLDVSRIA
                     YIMVLEENMNVPKEIIYAIGLLHDIGRWVEYEGGEKHNKASYKLSLDILKECDFNKEE
                     IEIVLSGILNHRNSEAEGLDKIIYLADKKSRSCFLCNAEKLCKWSKEKKNLDIII"
     gene            1615..2424
                     /gene="folP"
                     /locus_tag="K8M77_002335"
     CDS             1615..2424
                     /gene="folP"
                     /locus_tag="K8M77_002335"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBI7086788.1"
                     /translation="MKIGNKEFEIGKRTYIMGILNVTPDSFSDGGKYNDIELALKRAE
                     KMINDGVDIIDIGGESTRPTHTPVGEEEELNRVVPVIKALREKFDIPISVDTYKGKVA
                     EEAIKAGADLINDVWGFKKDKYMATVAAKYGVPCCIMHNRNDNEYGDDIIETMKSDME
                     ESIKIALDAGVKKENIILDPGIGFAKDYDQNLEVLNRLDEFCNMDYPVLLGTSRKSVI
                     GNTLNLPTDERLEGTLATTTLGIIKGCEFVRVHDVLENKRTALMTDAMVRR"
     gene            2437..3258
                     /gene="folK"
                     /locus_tag="K8M77_002336"
     CDS             2437..3258
                     /gene="folK"
                     /locus_tag="K8M77_002336"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBI7086789.1"
                     /translation="MDKIIIKDFEVFGNHGVFKEEKRLGQKFVLSIELFLDTREAGVT
                     GDLSKSVHYGELAHKVEEEFKKQSYDLIETAAEKLCEFILLEYPLVKKVKVSLKKPWA
                     PILRSLDTVSIEIERGWNEAYLSYGSNIGDKKYYIEEALKEINKAYHTEIIKKSNLIE
                     TEPWGYTEQDEFLNGACKIKTLLNPKELIKFLLSIEQKLKRERKIKWGPRTIDLDVIF
                     FNDLISEDEEIILPHPRMHERSFVLEPLNEIAPYKIHPLYRKRVFELLEELNKNK"
     gene            complement(3329..>3735)
                     /locus_tag="K8M77_002337"
     CDS             complement(3329..>3735)
                     /locus_tag="K8M77_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4883 family protein"
                     /protein_id="HBI7086790.1"
                     /translation="SKINSSSKEKPSLNYHTKNLSELVSKNNIKIRLLDMNIYSEVIV
                     DNEDIRIIDDLLKSLKDSNFINEEPLPNKPLYKIFIDLNSEKYVIDIYGDDLITLYPW
                     DSDVRKDYLSLKDIPNSFKLEPFCQYVFNKKQ"
BASE COUNT         1492 a          356 c          707 g         1180 t
ORIGIN      
        1 taaagcttta aaaataactc ttactgaaaa aaatataatt tattcaacta gagatattcc
       61 ttatggcata gaagattaca atagaggatt ttccataaag cttagtaaag taagaagaaa
      121 tagtacctct aagttaactt atttaaagag cacttgctat attgaaaata ttatggaaaa
      181 ggaaaaggct aaagatgaag gctttgatga agtgatattt ttaaatgaaa gaggagagct
      241 aacagaaggg gctactagta atatatattt tattaagaat aataagatat gtactccaaa
      301 ggttagctgt gggcttttag atggaataat tagaggatgg ataaagaata actttcaagt
      361 tgaagaagga tgttataata tagaagactt aagaaatagt gaagggattt ttataagtaa
      421 ttctcttctt ggaataatga gagtaagtca atttgaagaa gaattttttg gggaaaaaga
      481 tttaatagaa gaaataaaaa gtaaatatga aagttctatt ataagctttt aaatatggag
      541 ggcaaaatga tcgataaaga taagataaga gaaggaatga aactaatact tgaagcaata
      601 ggagaagatg ctaatagaga gggcttaata gagactccag atagaattgc aagaatgtat
      661 gaagaaatat tttcaggtat aggaatgaat gcaaaggagc atttaagtaa aacttttgaa
      721 gttcatagca atgatcttgt tttagaaaag gatataactt tctattctat gtgtgagcat
      781 catttagttc ctttctatgg aaaagttcat atagcatata taccaaatgg aagggttgta
      841 ggtttatcta aacttgctag atgtgttgag gtttattcaa aaaaaccaca acttcaagaa
      901 aggcttacaa cggaaatagc tgattctata atggaatact tagatgcaca aggtgtaatg
      961 gtagttgtag aaggtgaaca tatgtgtatg accatgagag gtgttagaaa accaggtgca
     1021 aaaacagtaa caacaactta tagaggaaag ttcttagaag atgaaagctt aaaaaatgat
     1081 gtttttagaa tgatttctat gtagatatta ttctttgagg tgtttattat ggagagaata
     1141 aataagatac ttaataatag taagtataaa gattatttaa ataggaattc tttttgtgaa
     1201 aaggataggg ttttttgcaa acataactta gagcattttt tagatgtatc aaggattgcc
     1261 tacataatgg tcttagagga aaatatgaat gtacctaagg aaattatata tgcaatagga
     1321 cttctccatg atataggacg atgggtagaa tatgaaggtg gagaaaaaca taataaggct
     1381 tcttataaat tgagtttgga tattttaaag gaatgtgatt ttaataaaga ggaaatagag
     1441 attgttttaa gtggaatttt aaatcacaga aatagtgaag cagaaggatt agataaaatt
     1501 atttacttag ctgataaaaa atcaagaagc tgttttttat gtaatgctga aaaactttgt
     1561 aagtggagca aagaaaaaaa gaatttagat ataattattt aagggggaac ttttgtgaaa
     1621 ataggaaata aagagtttga aataggcaaa agaacatata taatgggaat tttaaatgtg
     1681 actccagatt cattttcaga tggtggaaaa tataatgata ttgagttagc tctaaagaga
     1741 gctgagaaga tgattaatga tggcgttgac ataatagata ttggaggaga gtcaacaaga
     1801 ccaactcata caccagtagg agaagaagaa gaattaaata gagtagtacc tgtaataaaa
     1861 gccttaagag aaaagtttga tatacctata tcagttgata catataaggg gaaagtggca
     1921 gaggaagcta taaaagcagg tgcagattta ataaatgatg tatggggctt taaaaaggat
     1981 aaatatatgg ctactgtggc tgctaaatac ggagtacctt gttgcataat gcacaataga
     2041 aatgataatg aatatggtga tgacatcata gaaactatga aatctgatat ggaagaatct
     2101 ataaagatag ccctagatgc tggagttaag aaagaaaata taattttaga cccaggtata
     2161 ggttttgcaa aggattatga tcaaaactta gaagtattaa atagactaga tgagttttgc
     2221 aatatggact atccagttct attaggaaca tcaagaaaat cagtaatagg caatacttta
     2281 aatcttccaa cagatgaaag attagaaggt actttagcta caactactct tggaataatt
     2341 aaagggtgcg aatttgtaag agttcatgat gttttagaaa ataagagaac tgcccttatg
     2401 actgatgcta tggtaagaag ataggagtta attttaatgg ataaaattat tattaaagat
     2461 tttgaggttt ttggtaatca tggagtcttt aaagaggaaa aaagacttgg acaaaaattt
     2521 gttttaagca tagaactttt cttagatact agagaggctg gagttactgg ggatttaagt
     2581 aaatctgttc attatggaga acttgcacat aaggttgagg aagagtttaa aaagcaaagc
     2641 tatgatttaa tagaaactgc agcagaaaaa ttatgtgagt ttatattatt agaatatcct
     2701 cttgtaaaaa aagtgaaagt ttctttaaag aaaccatggg cacctatatt aagaagttta
     2761 gatactgtat ctatagaaat agagagagga tggaatgagg catacctttc atatggttct
     2821 aatataggag ataaaaaata ttatatagaa gaagctttaa aggaaataaa taaggcttat
     2881 catactgaaa ttataaaaaa atctaattta attgagacag aaccttgggg atatactgag
     2941 caggatgaat ttttaaatgg tgcttgtaaa ataaaaactc ttttaaaccc taaggaatta
     3001 ataaaatttt tattaagtat agaacaaaag cttaaaagag agagaaagat taagtggggt
     3061 cctagaacta tagacttaga tgtaatattt tttaatgatt taataagtga agatgaagag
     3121 atcatactgc cacatcctag aatgcatgaa agaagttttg ttttagagcc attaaatgaa
     3181 attgctcctt ataagattca tcccttatat agaaagcgtg tatttgaact tttagaagaa
     3241 ttaaataaaa ataaataaat ataaacaatc tattattaat aaagatatag aatttagtat
     3301 aaatcactac taaatcctat atcttttttt attgtttttt attaaaaaca tactggcaaa
     3361 atggttctaa tttaaaggaa ttaggaatgt cttttaaact taagtaatcc tttcttacat
     3421 cactatccca aggataaaga gttattaagt catcgccata aatatctatt acatattttt
     3481 cactgtttaa atcaataaaa attttatata aaggtttatt aggtaaaggt tcctcattta
     3541 taaagttaga atcttttaag ctttttaata agtcatcaat aattcttata tcttcattat
     3601 caacaattac ttcactataa atattcatat ctaataatct gatttttata ttattcttag
     3661 aaactagttc acttaaattt ttagtgtgat agtttaaaga aggtttttct ttagaagaac
     3721 tattaatttt agaac
//
LOCUS       DAEVGI010000191         3625 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEVGI010000191 DAEVGI010000000
VERSION     DAEVGI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.193"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            110..2746
                     /locus_tag="K8M77_002338"
     CDS             110..2746
                     /locus_tag="K8M77_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7086791.1"
                     /translation="MNYLEVKKDIYWVGSLDPELRVFDIIMYTPYGTTYNSYVVKGSE
                     KIAVFETVKEKCFDDYLSKLNSLGIDPKSIDYIVVDHTEPDHAGSVEKLLELSPKAKV
                     IGSMQAIEFLKDIVNKDFEYIVVGDNDTISLGNKTLQFISAPFLHWPDSMYTYIPEDK
                     ALITCDSFGSHYSCEEVFNDLVPNEEEYLDALRYYYDCIFGPYKPYVLKAIDKIKDLD
                     IELVCPGHGPILRQDPWKIINTYKEWSTPVKPKINGDKKVTIPYVSAYGYTKELAEEI
                     AKGIQSEHNVEVKLYDLTYSKQEDVLADIGESDGILFGSPTIVGELLPPIRILLANLN
                     PIIHGGKYAAAFGSFGWSGEAVPRIEARLKELKMNIFGPGLRIKFKPSTTELKEAFEF
                     GRDFSRTMFGEKELDYAPNNATETKEVLTGNGKTRYWKCLVCGEIFESPVCPEVCPVC
                     GAGKEQFIEVEMDTFEKNDTNDNFVIIGNGAAGFYAAKTIREINSTASVKLLSSEEVS
                     SYSRPNLSDLLNEEMNLDTFYLAKSSWYKENNIEELLGVTVTSIDKDAKKVILKDGTE
                     IPYTKLILANGSHNFIPPFRIKNNDEEVTLNCENIHEFKGIYSIKDLKDTFDVKEEMK
                     EAKKAVVIGAGPLGLEAAWEMKLAGLDVTVVEFLPNLMNNQLDQEGADIFKNQVSDCG
                     ITFITSEECTEIITKDSKLTSLKLKSGKTLDADILLVSTGIRSNIELAKETGIECNRG
                     VIVNERMETSVKDIYACGDVAEFNGLVYGNWPAAIEMGKTAGANACEAEKAFENFTPS
                     IILDALNTHVFSAGLIRFNDSSYEKISSKDESKGQYTKLFFKDNILVGGIIVGDISKA
                     GQIIIAIDNKLSKAAALSNDLL"
     gene            complement(2853..3098)
                     /locus_tag="K8M77_002339"
     CDS             complement(2853..3098)
                     /locus_tag="K8M77_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBI7086792.1"
                     /translation="MNYKTTGVCAKEINFEIEDNKIKSVSFVNGCPGNLMGVSKLVEG
                     IDVDEAISKLKGIDCRGKGTSCPDQLAKALEEYKKSL"
     gene            complement(3142..3522)
                     /locus_tag="K8M77_002340"
     CDS             complement(3142..3522)
                     /locus_tag="K8M77_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI7086793.1"
                     /translation="MAKEIIKTNKAPGAIGPYSQGIKIGDFVYTSGQIPLNPETGVMA
                     GSIEEQTAQSLENIKAILEAAGTSMDKVIKTTVFLSDMNDFVRMNEVYATYFTENQPA
                     RSAVEVAKLPKDAKIEIEVIATLK"
BASE COUNT         1312 a          484 c          574 g         1255 t
ORIGIN      
        1 atttacattt caaagaaaaa gaatataatt taattgtagg tattaattaa taattaatat
       61 tagttgattt tgattgttat aattttaaca tatattaggt ggtgttttta tgaattattt
      121 agaagtaaaa aaagatattt attgggttgg atctttagat cctgaattaa gagtttttga
      181 tattattatg tacactcctt acggaactac ttataactca tatgttgtta aaggtagtga
      241 aaaaatagct gtatttgaaa cagtaaaaga aaaatgtttt gatgattacc tatcaaaact
      301 taatagctta ggtattgatc ctaaatcaat agattacata gttgttgatc atacagagcc
      361 tgaccatgct ggatctgttg aaaaattact tgaattatct cctaaagcta aagttatagg
      421 ttcaatgcaa gctatagaat tcttaaaaga tatagttaat aaagattttg aatatatagt
      481 tgttggagat aatgacacaa tttcattagg aaacaaaaca ttacaattta tctctgcacc
      541 atttttacat tggccagatt caatgtacac ttatattcca gaggataaag ctttaataac
      601 ttgtgactct tttggaagcc actattcttg tgaagaagta tttaatgact tagttcctaa
      661 tgaagaggaa tatttagatg ccttaagata ctactatgat tgtatctttg gaccatataa
      721 accatatgtt ttaaaagcta tagataagat taaagattta gatatagaac ttgtatgccc
      781 aggtcatgga cctatcctta gacaagatcc ttggaagata ataaatactt ataaggaatg
      841 gagtactcca gtaaaaccta aaataaatgg agataaaaag gttactattc cttacgtatc
      901 agcttatggt tatactaaag aattagctga agaaatagct aagggaatac aatctgaaca
      961 taatgttgaa gttaaattat atgatttaac ttatagcaag caagaagatg tattagctga
     1021 cataggagaa tcagatggta tattatttgg atcaccaaca atagttggag aattattacc
     1081 tcctattaga attcttttag ctaacttaaa ccctattatt cacggtggaa aatatgctgc
     1141 agcctttggt tcatttggtt ggagtggtga agctgttcca agaatagaag ctagattaaa
     1201 agaattaaaa atgaatatat ttggacctgg tttaagaatt aagtttaaac catcaacaac
     1261 tgaattaaaa gaagcttttg aatttggtag agatttctca agaactatgt ttggagaaaa
     1321 ggaacttgat tatgctccaa ataatgctac agaaactaaa gaagtattaa ctggaaatgg
     1381 taaaacaaga tattggaaat gtttagtttg tggtgaaata ttcgaaagtc cagtatgccc
     1441 tgaagtttgc cctgtttgtg gagctggaaa agaacaattt atagaagtag aaatggatac
     1501 ttttgaaaag aatgatacta atgataactt cgttattata ggtaatggag ctgctggatt
     1561 ctacgctgct aaaacaataa gagaaataaa cagtacagct tctgttaaat tactttcaag
     1621 tgaagaagta tcttcttact caagaccaaa tctttcagat cttttaaatg aagaaatgaa
     1681 tttagatact ttctacttag ctaaatcttc ttggtacaaa gaaaataata tagaagaatt
     1741 actaggagtt actgttactt ctatagataa agatgctaaa aaagtaatct taaaagatgg
     1801 tactgaaata ccatatacta aacttatatt agctaatggt agccataact ttattccacc
     1861 atttagaatt aaaaacaatg atgaagaagt tactttaaat tgtgaaaata ttcatgagtt
     1921 taaaggtatc tattctatta aagacttaaa agatactttt gatgttaaag aagaaatgaa
     1981 agaagctaag aaggctgttg ttataggtgc tggtcctctt ggattagaag cagcttggga
     2041 aatgaaactt gctggattag atgttacagt agttgaattc ttaccaaatc ttatgaataa
     2101 ccaattagat caagaaggtg ctgatatctt taaaaatcaa gtttctgatt gcggaataac
     2161 attcataaca tcagaggaat gtactgaaat aataactaaa gatagcaaat taacttcttt
     2221 aaaattaaag agcggaaaaa ctttagatgc tgatattctt ctagtttcaa caggtattag
     2281 aagtaatata gaattagcta aagaaactgg aattgaatgc aatagaggtg ttattgttaa
     2341 cgaaagaatg gaaacaagtg ttaaagacat atatgcttgt ggagatgtag ctgaatttaa
     2401 tggtcttgtt tacggaaact ggcctgctgc tatagaaatg ggtaaaactg ctggtgcaaa
     2461 tgcatgtgaa gcagaaaaag catttgaaaa ctttacacca tctataatat tagatgcttt
     2521 aaatactcat gtattctcag ctggtttaat aagatttaac gattcttctt atgaaaaaat
     2581 aagttctaaa gatgaatcaa aaggtcaata tactaaacta ttcttcaaag acaacatctt
     2641 agttggagga attatagttg gtgatatttc taaagcagga caaataataa tagcaataga
     2701 taataaacta tctaaagcag ctgctttatc aaatgattta ctataaataa aataaaaagt
     2761 gactatataa attttatata gtcacttttt tatttagaga aatatattaa acttaagcta
     2821 aaattgctta aactatatgc tctttgtgct tgttataagc tttttttata ttcttctaaa
     2881 gctttggcaa gttgatctgg acaagatgtt ccttttcctc tgcaatctat tccttttaat
     2941 ttagaaatag cttcatcaac atctattcct tcaacaagct tagaaactcc cattaagttt
     3001 cctggacatc catttacaaa gctaacactt tttattttgt tatcttcgat ttcaaaatta
     3061 atctccttag cacaaactcc tgtagtctta taattcataa gtaaaatctc ccctatattg
     3121 ctaattatga agatatagaa actattttaa agtagctata acttcaatct ctattttagc
     3181 atctttaggt aatttagcaa cctctacagc acttcttgct ggttgatttt ctgtaaaata
     3241 agtagcatat acttcattca ttcttacgaa atcattcata tcacttaaaa atactgtagt
     3301 ttttattact ttatccatag aagttcccgc agcttctaag atagccttta tattttctaa
     3361 ggattgagct gtttgttctt caattgatcc tgccattacc cctgtttcag gatttagtgg
     3421 aatttgtcct gaagtataaa caaaatctcc tatttttatt ccttgtgaat aaggtccaat
     3481 tgcacctggt gctttgtttg tttttattat ttcttttgcc atataaaaca tctcctattc
     3541 ttcaacacat tacttctgat actttaattt taatacagca aaaatttaaa ttcaatggat
     3601 tattactatt tttctaaact atttg
//
LOCUS       DAEVGI010000192         3543 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEVGI010000192 DAEVGI010000000
VERSION     DAEVGI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.194"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(291..770)
                     /locus_tag="K8M77_002341"
     CDS             complement(291..770)
                     /locus_tag="K8M77_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBI7086794.1"
                     /translation="MEIYDISVKDINGENVSLEIYRGKVLLIVNTASKCGFTKQFDGL
                     EELYEKYKDEGFEVLGFPCNQFKEQDPGSNSEIMNFCKLNFGVTFPMFEKIDVNGENE
                     SLLYSYLKEQKSGMFGSKIKWNFTKFLVDREGNVIKRFSPQTTPKSIEKDIEELLAK"
     gene            complement(1219..1845)
                     /locus_tag="K8M77_002342"
     CDS             complement(1219..1845)
                     /locus_tag="K8M77_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7086795.1"
                     /translation="MGFLESFVSFIVYLASTCGYGGIFILNMLEYACLPFPSEIVLPA
                     IGANLASGKYSMIYALPISIIGGVVGTLISYFVGMYAGKKVISFAKRKHKKSKCIFEK
                     IDRLFSRYGKITVFLARLLPFTRTYISLFAGAEKVNKIMFVIFSVLGITIMDSFYLLL
                     GYFAYDNKSFIEKALRHYSIALIIIIVIAIFSYYIYKNLILKKLKKEN"
     gene            complement(2206..2625)
                     /locus_tag="K8M77_002343"
     CDS             complement(2206..2625)
                     /locus_tag="K8M77_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086796.1"
                     /translation="MDQRSLEKKCKFNERVILFTFLLTIATLIAIWISTFKYYYNEIK
                     CNNVTMEKLPIGSFQEEYKSPSKKYTINSYICSSGGVPDWAVRCEVVNNISGEVRNIY
                     WDYKIKSIDVKWINDHIVLINGHKIDVKNEVYDWRKI"
     gene            complement(2711..3361)
                     /locus_tag="K8M77_002344"
     CDS             complement(2711..3361)
                     /locus_tag="K8M77_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBI7086797.1"
                     /translation="MNNIENIKDVFRDYKMGINGEEDMKRCSVLIPVVNIDGEDNIIF
                     EIRNNKLNSNPGEICFPGGTIEEGETPKEAALRECSEEIGLGEENLEIISQLDFYVSP
                     NNILIYPFLGVQKNQKEDIKKFISINKEEVCDILLVPFKYLLNYEPEITYSKIINIPK
                     EDFPFHNIIGGKDYKFRDGRYKVMFYKYNNFVIWGMTARILENFLNVYKGHYNKNI"
BASE COUNT         1260 a          553 c          265 g         1465 t
ORIGIN      
        1 ataaaaatgt attaaaattt aaacaataca aatatttata aaaaaacacc aaatctattt
       61 tttaaattta gtattttcta tgcataagac tttcaaataa actcaaaaat atataaataa
      121 tttctatcct atttcctttg ctattaataa tttatattaa tactctaaaa tctactcata
      181 tttgtattat gcttataaat tttcaaagtc ttataaaatt ttattgctat aaaaaataaa
      241 atacaatggc ctaaatttag taccattgta ttttctactt aaatcacttt ctatttagct
      301 aaaagctcct caatatcttt ttctattgat tttggagttg tttgtggcga aaatctcttt
      361 attacatttc cctctctatc cactagaaac ttagtaaaat tccacttaat tttacttcca
      421 aacattccac ttttctgttc ttttaagtaa gaatataata gagattcatt ttctccatta
      481 acatcaattt tttcaaacat agggaatgtt acaccaaaat ttaatttaca aaagttcatt
      541 atttctgaat tgcttcctgg atcttgttcc ttaaattgat tacaaggaaa tcctaaaacc
      601 tcaaaacctt catctttata tttttcataa agctcttcta atccatcaaa ctgttttgta
      661 aaaccacatt tactagcagt attaactatt aaaagaacct ttcctctata tatttctaaa
      721 cttacatttt ctccattaat atcttttacg ctaatatcat aaatttccat actactcacc
      781 ccatatattt taaattatat tttttaaaat ttaattttat acaattattt taattctata
      841 tacttaatat gtcaatatac atttgcatta atctaaaaat atttttccat tttttagtaa
      901 taattttaaa attctctgca tataaatatc atagtagggg agttttttga taatgtacta
      961 atttgtctgc ttcttcataa attatgcaaa aaaaacttct cacagcacaa tattcgccaa
     1021 actctacatc aaataaaatc ttaatattta cctaacctaa taccaaaata gagcttactt
     1081 ttattaaaaa gtaagctcta tttctttata acaatcatga taatttctta ttttattcta
     1141 attttgttaa atatttagaa ttatatattg tgtatacaac ttaataaatg acaacttata
     1201 aaaacttgtc aataaaactt aattctcttt tttaagtttc tttaatatta gatttttata
     1261 aatataatat gaaaaaatag ctattactat tataattatt aatgctattg aataatgtct
     1321 taaagctttt tctataaatg atttattatc atatgcaaaa tatcctaata gaagatagaa
     1381 actatccatt attgttatac ctaaaactga aaatataaca aacataattt tattaacctt
     1441 ctcagctcct gcaaaaagtg atatatatgt tcttgtaaat ggtaataatc tagctaggaa
     1501 aactgttatt ttgccatacc tagaaaataa cctatctatt ttttcaaata tacatttact
     1561 ttttttatgc tttctcttag caaatgaaat tacttttttt cctgcataca ttcccacaaa
     1621 atagcttatt agggtaccaa ctacacctcc tataatggat attggcaaag catatatcat
     1681 gctatatttg ccacttgcta aattagcacc tattgcaggt aatacaattt cacttggaaa
     1741 tggcaaacaa gcatattcaa gcatgtttag tataaatatt cctccgtatc cacatgtgct
     1801 agctaaataa actataaagc taacaaaact ctctaaaaac cccataaact attatccttc
     1861 ccatctgttg tattaattta taaactaatt cttaaatatg tgtttttaca tataaataat
     1921 caatatcttt aagttattat aatactccat aaataataat aacaaactat tctttttaat
     1981 ttattactaa aaagtttctt atttttgaaa acattttctt gcatttttaa aaatattata
     2041 ttataattaa tgtaaaggtt ttatcgatga aaccctcttt tcttacttta tcatcgataa
     2101 aagcaattat ttttttcatt tttaatgaac ttttgtgtta tttacgtatt attgaatgtg
     2161 attaaaaaag acaaatactt ttagtatttg tcttttttat tttagttata tttttctcca
     2221 atcataaacc tcatttttaa catctatttt atgtccattt attaagacta tatgatcgtt
     2281 aatccatttt acatcaattg attttatctt ataatcccaa tatatatttc ttacttctcc
     2341 acttatatta ttaaccactt cacatcttac ggcccaatca ggtactcctc cagagctaca
     2401 tatgtaagaa ttaatagtat attttttgct tggagattta tactcttctt gaaagcttcc
     2461 tatgggtaat ttctccatag taacgttatt acacttaatt tcattgtaat aatacttaaa
     2521 tgtagaaatc caaattgcta ttaatgtagc aatagtaagt aaaaatgtaa atagaataac
     2581 cctttcatta aacttacact ttttctctaa acttctttgg tccacttaat cgctccttta
     2641 aaatgtctat atatttataa aatttaacaa aaacctgttt taatacaagt tttattttta
     2701 aattaatata ttaaatattt ttattataat gtcctttata tacatttaag aaattttcta
     2761 atattctagc agtcattccc caaatcacaa aattattata tttataaaac ataactttgt
     2821 atcttccatc tctaaattta taatctttac cacctattat attatgaaaa ggaaaatcct
     2881 cctttggtat atttataatt ttgctgtagg taatttcagg ttcataattt aataaatatt
     2941 taaaaggaac taataatata tcacatactt cttctttgtt gattgaaata aattttttta
     3001 tatcctcttt ttgatttttt tgaactccta aaaaaggata aattaatatg ttgttaggtg
     3061 aaacataaaa atctaactga cttataattt ctaaattttc ttctccaaga ccaatttcct
     3121 cagaacattc tcttaaagca gcttctttag gagtttctcc ctcttctatg gtaccaccag
     3181 ggaaacatat ttcacctgga ttactattta atttattatt tcttatttca aaaataatat
     3241 tgtcttcccc atctatattt acaactggaa ttaaaacaga acatcttttc atgtcctctt
     3301 ctccatttat acccatttta taatctctaa acacgtcttt tatattctca atattattca
     3361 taatataatt cacctcttaa taccactaat gtattttcaa tacttatcat caatattata
     3421 aaattaaatt tatttttatt acattgagtt tttttatttt atttattggt ttaagaaatt
     3481 tctaataaat ttaactataa aattaactct attaacctaa cttcaattat tttatcataa
     3541 tat
//
LOCUS       DAEVGI010000193         3476 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEVGI010000193 DAEVGI010000000
VERSION     DAEVGI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.195"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            147..1127
                     /gene="manX"
                     /locus_tag="K8M77_002345"
     CDS             147..1127
                     /gene="manX"
                     /locus_tag="K8M77_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBI7086798.1"
                     /translation="MVGIILASHGEFAKGILQSGSMIFGEQENVKAVTLMPSEGPDDI
                     KAKMKEAIASFDNQDEVLFLVDLWGGTPFNQANSLVEEHADKWAIVAGMNLPMVIEAY
                     ASRFSMESAQEIAVNILKSARDGVKVKPESLEPEEDTKTNTDSAQQSNNVGAPGSFEY
                     VLVRIDSRLLHGQVATAWTKTVKPTRIIVVSDDVAKDELRKKLIQQAAPPGVKAHTVP
                     VSQMIKLAKDDQHFGGQRALLLFENPEDVLRAVEGGVPIKTVNVGSMAHSPGKVQPNK
                     VLAFDQEDIDTFKKLKEAGLDFDVRKVPNDTKGNMDEILKKAQEELNKLK"
     gene            1176..1979
                     /locus_tag="K8M77_002346"
     CDS             1176..1979
                     /locus_tag="K8M77_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBI7086799.1"
                     /translation="MDLNIIQIILVIIIAFLAGMGGILDEFQFHQPLVACTLIGLVTG
                     NLIPCLILGGSLQMIALGWANIGAAVAPDSALASVAAAIILVLSGEGQAGISSAIAIA
                     IPLAVAGLLLTIICRTIATAFVHFMDAAAKEGDIRKVEMWHFIAILMQGVRIALPAAL
                     ILLIGAGPISALLASMPAWLTDGLAVGGGMVVAVGYAMVINMMATKEVWPFFAIGFVL
                     ATIPQLTLIGLGAIGVALALIYLALSKKGGSGNSGSNIGDPVGDIIDRY"
     gene            1999..2910
                     /locus_tag="K8M77_002347"
     CDS             1999..2910
                     /locus_tag="K8M77_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014091655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBI7086800.1"
                     /translation="MEKKLKLTKKDRLSIWWRSTFIQGSWNYERMQNGGWAFSLIPAI
                     KKLYKTKEERSAALTRHLEFFNTHPYVASPIIGVTLALEEDRANGAQVDDVTIQGVKV
                     GMMGPLAGIGDPVFWFTVRPILGALGASLAMAGNILGPIIFFLGWNIIRMAFTWYTQE
                     FGYKAGSRITEDLSGNLLQDITKGASILGMFILGSLVNRWVSIKFTPVVSTVQLSPGA
                     YIDWNSLPAGAEGIKQALLQQSSGISLTAEKVTTLQGNLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIVIILALFIIGIIFHVIGLM"
     gene            3066..3440
                     /locus_tag="K8M77_002348"
     CDS             3066..3440
                     /locus_tag="K8M77_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBI7086801.1"
                     /translation="MVQSLNTKVDLVVDATSFSGLTDYGQIMIGDKGFEFYNSNDYRK
                     FIQIPWEEVDYVIASVMFKGKWIPRYAIKTKKNGTYSFSSKDTKKVLRTVRKYVNANR
                     IVKSLSFFDVVKRELKALFKKK"
BASE COUNT         1212 a          467 c          673 g         1124 t
ORIGIN      
        1 tatttaataa aatttatatt taaaaaaagt atatatgtgt ttaaaatttg cataaggctt
       61 gtaaaagtga tgtgaaaatt gtaaaattaa cgtaagataa agggaaaaat ttgtcttaaa
      121 aaataaaaaa aataggaggt atagatatgg taggaattat tcttgctagt cacggagaat
      181 ttgctaaagg aatattacaa tctggctcaa tgatttttgg agaacaggaa aatgtaaaag
      241 ctgttacatt gatgcctagc gaaggaccag atgatattaa agcaaagatg aaagaagcaa
      301 ttgcttcctt tgataatcaa gatgaggttt tattcttagt tgacctatgg ggtggaacac
      361 catttaatca agcaaacagc ctagtagaag aacatgcaga taaatgggca atcgtagctg
      421 gaatgaattt accgatggtt attgaagctt atgcttcacg tttttcaatg gaatcagcac
      481 aagaaattgc tgttaatatt ctaaagtcag ctagagatgg agttaaagtt aagccagaat
      541 cattagaacc agaagaggat actaaaacaa atacagattc tgcacaacaa tctaataatg
      601 taggtgcacc tggctctttt gaatacgttt tggtacgtat tgattcacgt ttacttcatg
      661 gtcaagtagc tactgcttgg acaaaaactg taaaaccaac aagaattatt gttgtatcag
      721 acgatgtagc taaagatgaa cttcgtaaga aattgattca acaagcagct cctcctggag
      781 ttaaagcaca tactgttcca gttagccaaa tgattaagct tgcaaaagat gaccaacact
      841 ttggaggaca acgtgcgtta cttctttttg aaaatccaga agatgtacta agagcagtag
      901 agggaggagt acctataaag acagttaatg ttggttctat ggctcactct cctggaaagg
      961 ttcaaccaaa taaagtactt gctttcgatc aagaagatat tgatactttt aagaagctta
     1021 aagaagctgg attggatttc gatgttcgta aagttccaaa tgatacaaaa ggaaatatgg
     1081 acgaaattct taaaaaggca caagaggaat taaataaatt aaagtaattt tttaacatag
     1141 ataaattatt ttaatgaaaa ggaggattaa taacagtgga tttaaatata attcaaataa
     1201 tattagttat tattattgca ttcttagctg gtatgggagg tatcctagat gaattccaat
     1261 ttcatcaacc gttagttgct tgtactttaa taggtttagt tacaggcaat ttaatacctt
     1321 gtttaatact aggtggtagt cttcaaatga tcgctttagg ttgggcaaat ataggtgctg
     1381 ccgtagcacc agattcagca ttagcatctg ttgcagccgc aattatctta gttcttagtg
     1441 gagaaggtca agctggaata tcttcagcca tcgctatagc aattccacta gctgttgctg
     1501 gattattatt aacaattatt tgtcgtacaa tagctacagc atttgtacat tttatggatg
     1561 ctgctgctaa agaaggtgat ataagaaaag ttgaaatgtg gcatttcatt gctattctta
     1621 tgcaaggtgt acgtatagca ttaccagctg cattaatttt attaataggt gctggtccaa
     1681 taagcgcatt acttgcttct atgcctgctt ggttaacaga tggtttagca gtaggtggtg
     1741 gaatggtagt agctgttggg tacgcaatgg taattaacat gatggcaaca aaagaagtat
     1801 ggccattctt tgcaatcggt tttgtattag caactattcc tcaacttaca cttataggat
     1861 taggtgcaat tggtgtagct ttagctctta tttacttagc acttagcaaa aaaggtggct
     1921 caggtaatag cggatcaaac ataggagatc cagtaggcga tattatcgat agatactaag
     1981 aagggagaga ggacgaaaat ggagaaaaaa ttaaaattaa caaaaaaaga tcgtctttct
     2041 atttggtggc gttcaacttt cattcaagga tcttggaact atgaaagaat gcaaaatggt
     2101 ggttgggcat tctcattaat tcctgcaata aaaaaattat acaaaactaa agaagaaaga
     2161 tcagcggcat taacacgtca cttagaattt ttcaatactc atccctatgt agcttcacct
     2221 ataattggtg taacattagc attagaagaa gatcgtgcaa atggagctca agtagatgat
     2281 gtaactattc aaggggttaa agttggtatg atgggacctt tagcaggtat tggagaccct
     2341 gtattctggt ttactgttag acctatatta ggagcattag gtgcttcact tgctatggct
     2401 ggtaacatat taggaccaat aatattcttc ttaggttgga atattatccg tatggcattt
     2461 acttggtata cacaagaatt tggttataaa gccggctctc gtattacaga agatttatca
     2521 ggaaacttat tacaagatat tacaaaaggt gcatctatac ttggtatgtt tatactagga
     2581 tcattagtta atagatgggt atctattaaa tttactccag tagtatcaac agttcaatta
     2641 agcccaggtg cttatattga ttggaatagt ctaccagcag gtgcagaagg tattaagcaa
     2701 gccttattac aacaatcatc tggaatttct ttaacagctg agaaagttac aacattacaa
     2761 ggaaacttag attcattaat tcctggtctt gcaggattat tattaacatt cctttgtatg
     2821 tggttactta agaagaaggt atctccaatt gtaattattc ttgctttatt cataattggt
     2881 attatattcc atgtaatagg attaatgtaa tattttgttt agcctaggtt agtataatat
     2941 tttacttagc ctaggctttt cttaagaata agagtttaaa agaaagtttt tataaattct
     3001 atttattttt aaaataaata gttaagtaaa ataagtatat atttaattag aaaagaggta
     3061 gatctatggt tcaatcactt aatacaaaag tagatttagt agttgatgca acatcctttt
     3121 caggtctcac agattatggg caaataatga ttggtgataa agggtttgaa ttttataatt
     3181 ctaatgatta tagaaaattt attcaaattc cttgggaaga agttgactat gtaattgcat
     3241 cagttatgtt caaaggaaag tggattccac gatatgcaat taaaactaag aaaaatggta
     3301 catattcatt ttcttctaaa gacacaaaga aagtacttcg tactgttaga aaatatgtta
     3361 atgcaaatcg tatagttaag tcattaagtt tctttgatgt tgtaaaaaga gagttaaaag
     3421 cattatttaa gaaaaaataa tttaaagttt tttctgcaat aaatttagaa aattaa
//
LOCUS       DAEVGI010000194         3476 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEVGI010000194 DAEVGI010000000
VERSION     DAEVGI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.196"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            76..1212
                     /locus_tag="K8M77_002349"
     CDS             76..1212
                     /locus_tag="K8M77_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBI7086802.1"
                     /translation="MKELERLLGRLTIINIKEIIIILVIMIIVLIINKILINNLFKLL
                     IRGARRTKNYLDNNIVRALENPLKLIVSFVGIYSIFKVINLDSLGIDFLSTYKILRVG
                     IIVSFIYFAYNLTLENSLLYSKLHKNDGGNTIVFPFVSIIIRLIILLVGIIIIANEFG
                     LTGFIAGLGVSGVAFALAAQDTCSNLFGGMVIVLDRPFSIGDWIQAGDVEGVVEEITF
                     RSTRIRTFSMAIATVPNSKLANSNIINWTQRKLRRIHFKFTMDCKTPIESIKNSVNRI
                     ENMLKKHDKIDKNLIIVSFNELSTYGFGVFIYFYTDEFEYLKYEKIKEEINIKILEIL
                     REENVSLMFLNFDFKDFERKSGNCNLENTELESIKNITEEERGI"
     gene            1209..1832
                     /locus_tag="K8M77_002350"
     CDS             1209..1832
                     /locus_tag="K8M77_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBI7086803.1"
                     /translation="MKIKLEACVESYKEGKRAEELGADRIELCDNLAQGGTTPSYGTI
                     LQCKKDINIDINVIIRPRGENFVYSKEELNIMKNDIKLCKDIGVTGVVFGFLKEDNTL
                     DYEATKEFVELAKPLSVTFHMAFDEIVNKFDALDKLIDLGVDRVLTKGGLDNSFKNKD
                     TLKELVEHAKDKIIILPGGGVTKDNFLELKDYTNAREFHGTKIVGNL"
     gene            complement(2217..2510)
                     /locus_tag="K8M77_002351"
     CDS             complement(2217..2510)
                     /locus_tag="K8M77_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086804.1"
                     /translation="MKNYKDNDENFKLVPMNMKCNQEYSFMPMNKLNSNSININPMIN
                     NNSNHSCCCMNNCLSKLSPYNNYCENKISSENYTGNMIVKTLITVKKKSDLFD"
     gene            complement(2716..3393)
                     /locus_tag="K8M77_002352"
     CDS             complement(2716..3393)
                     /locus_tag="K8M77_002352"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBI7086805.1"
                     /translation="MAAEFNNDWDDLLKDEFEKEYYLNLRKFLIDEYKTQKIHPSMYD
                     IFNALKFTPYKDVKVVILGQDPYHGPNQAHGFSFSVKPGVQTPPSLRNMFKELNSDLG
                     CYIPNNGFLESWAKQGILLLNTVLTVREGQANSHKGKGWEIFTDRVIELLNKREEPIV
                     FILWGRNAISKETLITNPIHKIIKSVHPSPLSATRGFFGSKPFSKTNDFLVSINKEPI
                     DWQIPNI"
BASE COUNT         1312 a          380 c          540 g         1244 t
ORIGIN      
        1 tttttatatt caaatttaaa ataaattagt catttcagga aaagataaaa agaagaatat
       61 cttttgaggt gtgctatgaa ggaattagaa agacttttag gaaggctaac aattatcaat
      121 ataaaagaga ttattataat tcttgttatt atgataattg ttttaatcat aaataaaata
      181 ctaataaata atctatttaa gttactaatt agaggggcaa gaaggactaa aaattactta
      241 gataataata ttgttagagc tttagaaaat ccattaaagc ttattgtatc ctttgttgga
      301 atatattcaa tatttaaagt tataaatctt gattccttag gtatagattt tttgtctaca
      361 tataagattc taagagtagg aataatagtt tcatttatat attttgctta taacttaact
      421 ttggaaaatt cattacttta ttctaagtta cataaaaatg atggtggtaa cactattgta
      481 tttccttttg tttctataat tattagatta ataatattgc tagttggaat aattataatt
      541 gcaaatgaat ttggcttaac tggttttatt gctgggttag gtgtaagtgg agttgccttt
      601 gctttagctg cacaggatac ctgttcaaat ttgtttgggg gaatggtaat agttttagat
      661 agaccctttt ctattggaga ttggatacaa gctggagatg ttgaaggggt tgttgaggag
      721 attacattta gaagtacaag gataagaacc ttttccatgg caattgctac tgtacctaat
      781 tcaaaacttg ctaatagcaa cataataaat tggactcaaa gaaaattaag gagaatccat
      841 tttaaattta ctatggattg caagacacca atagaaagta taaaaaattc tgttaataga
      901 atagaaaata tgctaaagaa acatgataaa atagataaaa atttgataat agtaagtttt
      961 aatgagcttt caacttatgg ttttggagtt tttatatatt tctacacaga tgaatttgaa
     1021 tatttaaaat atgaaaagat taaggaagaa ataaatataa aaatcttaga gatattaaga
     1081 gaagaaaatg ttagtttaat gtttttaaat tttgatttta aagattttga gagaaaatct
     1141 ggaaattgta accttgaaaa cactgagttg gaatccataa aaaatattac tgaggaggag
     1201 aggggaatat gaaaattaag ttagaagcat gtgttgaaag ttataaggaa ggaaaaagag
     1261 ctgaggaatt aggagctgat agaatagagc tttgtgataa cttggctcaa gggggaacaa
     1321 caccaagcta tggaacaatt cttcaatgta aaaaggatat taatatagac attaatgtaa
     1381 ttattcgacc aagaggagaa aattttgttt attctaaaga agaattgaac ataatgaaaa
     1441 atgatataaa actttgtaag gatatagggg taacaggagt agtatttgga tttttaaagg
     1501 aagataatac tttagattat gaagcaacaa aggaatttgt tgagttagct aaacctttaa
     1561 gcgttacatt tcatatggca tttgatgaaa tagtaaataa gtttgatgca ctagataaac
     1621 ttatagattt aggagtagat agggttttaa caaagggtgg tttagacaat tcttttaaaa
     1681 ataaagatac cttaaaagaa ttagttgaac atgctaaaga taaaataata atacttccag
     1741 gtggaggagt tactaaagat aattttttag agttaaaaga ttataccaat gctagggagt
     1801 ttcatggaac taaaatagta ggaaatttat aaaaacaata gaaatgatta gaaataactc
     1861 ttaatatata taattaatct tttaaaacag taaaaaataa ctctttacaa agtaaggtta
     1921 atattttaat attatatata atattaaaaa aaataaatga gtttgttcgg gcttatagta
     1981 taacattaac atagtgttaa tttcccccta cttaaactat aagccctttt taatataaaa
     2041 tttattaaac ttggaaaagc tgatagctta aactattggt atccaacgag gtcggaatgc
     2101 ccttgttagc ttggttaatc tcacaccgtt aggtgtgttg cctaagaatc ttggcatttc
     2161 aataccgagt agttcaaatg ctagtattag attgatatgt agagatacta catatattaa
     2221 tcaaacagat cagatttctt ttttactgtt attaatgttt ttactatcat attgcctgta
     2281 taattctcag aagaaatttt gttttcacaa taattattat aaggtgatag cttcgacaaa
     2341 cagttattca tacagcaaca actatgattt gaattattat taatcatagg attaatatta
     2401 attgaatttg aattcaattt attcataggc ataaaagaat attcttgatt acatttcatg
     2461 ttcataggaa ctaatttaaa attctcatca ttatctttat aatttttcat aaaattacac
     2521 ccctaactta attaatactt ctaatagagt atattaagtt aggggtgtaa agtttcctta
     2581 aaaatcaatt tagtttatat aaatcaatat aaatttaaaa tttcaatttc aaaagctaga
     2641 ttaggtattg ataaagagtt ttatataaat caatgtaaac ttaaaataat cattaatatt
     2701 aagtataaag tttggttata tattaggtat ttgccaatct ataggttctt tatttattga
     2761 aactaaaaaa tcattagttt tagagaatgg ttttgaacca aagaatccgc gagtagcaga
     2821 tagtggactt ggatgcactg attttattat tttatgtatt ggattagtta tcaaagtttc
     2881 ttttgaaata gcatttcttc cccaaagaat aaatactata ggttcttctc ttttgtttaa
     2941 caattcaatt actctatcag tgaatatttc ccaaccctta cccttatgag aatttgcttg
     3001 cccttctcta actgttaaaa cagtatttaa taaaagaata ccttgtttag cccaagattc
     3061 caaaaatcca ttgttaggta tgtaacatcc aaggtcagaa tttaattctt taaacatgtt
     3121 tcgcaaagat ggaggagttt gaactcctgg tttaacagaa aagctaaatc catgagcttg
     3181 gtttggtcca tgatatggat cttgacctaa aataacaacc ttaacatctt tgtaaggtgt
     3241 gaattttaat gcgttaaata tatcatacat gcttggatgt attttttgag ttttatattc
     3301 atctataaga aactttctaa gatttaagta atattccttt tcaaactcat cttttaataa
     3361 atcatcccaa tcattattaa attcagcagc catttaatta cctccttata taaaaataaa
     3421 ttttgccaaa atcataatta aattttatac ttaataattt cttataacaa gggtgc
//
LOCUS       DAEVGI010000195         3463 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEVGI010000195 DAEVGI010000000
VERSION     DAEVGI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.197"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(83..382)
                     /locus_tag="K8M77_002353"
     CDS             complement(83..382)
                     /locus_tag="K8M77_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086806.1"
                     /translation="MLIYYCISLMMLLTMLMTGIINPICSFLSGDTEIRFNGILYTNL
                     NEEKYKKILFSTFLNFIILILCLCIVCFKFNPFFPIFLAILCLIFLQHYILKSLK"
     gene            874..2964
                     /locus_tag="K8M77_002354"
     CDS             874..2964
                     /locus_tag="K8M77_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBI7086807.1"
                     /translation="MSKHLATTIRVAIEKDNPSICRDEEKCIKCGSCKNICTDYIGVN
                     GHYSLEKTNDIAVCINCGQCANVCPTSSITEVFDYKKVEDAINDKDKIVIVSTSPAVR
                     VSLGEEFNMDDGSFVQGKMIALLRKLGFKYVLDTNFAADMTIVEEASELVERITKNNK
                     PLPQFTSCCPAWIKYAETFHPEILDHISTSKSPIGMQGPTIKTYFAKNMGIDPSKIIN
                     VALTPCTAKKFEIKREEMNASGRYYGIEDMRDMDYVITTREVAIWAKEKGIDFNSLED
                     SNFDKLMGEASGAGVIFANTGGVMEAALRTAYKYITKKEPPKDFYDLEAVRGMEDIRE
                     ASFKINDLDINVAVIYGTENASKFISKMKDSDKKYHFVEVMTCPGGCIGGGGQPKDKN
                     LQGDKLREKRIDGLYRRDSSMKLKVSCENEEIKNLYKEFYGKPLSDLAEKMLHTTYSD
                     RSSDLGGENMSESVKYRCTVCGYIQEGELPEGFICPVCKSPASVFVKVEEKTEVDDKE
                     DNKSKSTNESSNKYKGTKTEKNLMDALAGESIARNKYTFFADVAKNEGYEQIYKLFLK
                     TAGNEREHAKLWFKELGYLGDTQENLLHGSEGEHYEWSDMYARFAKEAEEEGFFDLAE
                     KFVEVAKIEKSHEDRYRKLLNNIKMKKVFEKSEETMWECLNCGYLVMGKKAPEVCPVC
                     NYAEGFFEVRAENY"
     gene            complement(3104..3403)
                     /locus_tag="K8M77_002355"
     CDS             complement(3104..3403)
                     /locus_tag="K8M77_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086808.1"
                     /translation="MKSLMSFIPMILSLAITTFIFIPINKSLKLSDKISKIIPTTPKF
                     KPLFFVVCMFLLLLIIGLLGLYVIPMNNLTYYILTGIIAGIGISITVEISPKHHK"
BASE COUNT         1416 a          313 c          611 g         1123 t
ORIGIN      
        1 ttgataaatt ataagatatt aagaaataaa aataccccat agaatagaca atttaaaaag
       61 tctattctac ggggtattct tattatttta atgattttaa tatatagtgt tgtaaaaata
      121 ttaagcacaa tatagctaag aatataggga agaaaggatt aaatttaaaa catactatac
      181 atagacataa tattaaaata ataaaattta aaaaggttga aaataatatc tttttatatt
      241 tttcttcatt taaattagta tatagaattc cattaaatct aatttcagta tcaccactaa
      301 ggaatgaaca aattggatta ataattccag tcataagcat agttaaaagc atcattaaag
      361 aaatacaata atatataagc atctacatct ttcctcctta cttagaatat ctatttaaat
      421 tttaatctta tattaaaaaa taataataaa ttaatcaata ggttttgtgt aactggtcga
      481 aaagttaaca aaattggcat aaaagtaagt aaatgtatta atagatgatt aataaagtat
      541 gaattttaga taattaatat ataagtaata ttaattttta gagatgaata aagtatggag
      601 tagactttaa agtatattgc agtaatttat tatatttata gattaaaaat atataaatta
      661 aaatttatag ataaaaacct tatagaattg aaagtgtgtt aaagcaaagt taatattgtt
      721 aataaaaagt taataaatac ttatgaaatg attaatagta tttaaattca ttaattgaaa
      781 aaatatgtat agtaggttaa tattatatga aataaataaa aaattaaatt tacaaaataa
      841 gtagaagttt aaattaaggg gggggaaaaa tctatgagta aacacttagc tacaactata
      901 cgtgtagcta ttgagaagga taatccatct atttgtagag acgaagagaa gtgtataaaa
      961 tgtggttcat gtaaaaatat atgtacagat tatataggtg taaatggtca ttattcttta
     1021 gaaaaaacta atgatattgc tgtttgtata aattgtggtc aatgtgcaaa tgtatgccct
     1081 acatctagta ttaccgaagt ttttgattat aaaaaggtag aagatgcaat aaatgataaa
     1141 gataaaattg taatagtttc cacatctcca gcagttagag tatctttagg tgaagagttt
     1201 aatatggacg atggaagttt tgttcaaggt aaaatgatag cattgcttcg taagttaggt
     1261 tttaaatatg ttcttgatac taattttgca gctgatatga ctatagtaga ggaagctagt
     1321 gaattggtag aacgtataac taaaaataat aaaccgttac ctcaatttac tagttgttgt
     1381 cctgcttgga taaaatatgc tgaaactttc cacccagaga tattagatca tatttcaact
     1441 tcaaagagtc ctataggaat gcaaggtcct actataaaaa cctattttgc aaagaacatg
     1501 ggaattgacc cttctaagat tataaatgta gctttaactc catgtacagc taaaaaattt
     1561 gaaataaaaa gagaagaaat gaatgcatca ggtagatatt atggcataga agatatgcgt
     1621 gatatggatt atgttataac aactagagaa gtggctattt gggctaaaga aaaaggaatt
     1681 gattttaatt ctttagagga tagtaacttt gataaattaa tgggagaagc atcaggggct
     1741 ggtgttatct ttgctaatac aggtggagtt atggaagctg ctttaagaac tgcatataag
     1801 tatataacta aaaaagaacc acctaaagat ttttatgatt tagaagcagt aagaggtatg
     1861 gaagatatac gtgaagctag ttttaaaata aatgacttag atataaatgt agctgttata
     1921 tatgggactg aaaatgcttc aaaatttatt tcaaaaatga aagattctga taaaaaatat
     1981 cattttgttg aagtgatgac ttgtccaggg ggatgcattg gtggaggtgg acaacctaag
     2041 gataagaatt tacaaggtga taagcttaga gaaaagcgta tagatggttt atatagaaga
     2101 gattctagta tgaaattaaa agtcagttgt gaaaatgaag agattaaaaa tttatataaa
     2161 gagttttatg gaaaaccttt aagcgatctt gctgagaaaa tgttacatac aacttattca
     2221 gatagaagta gtgatttagg aggagaaaat atgagtgaat cagtaaaata tcgttgtaca
     2281 gtttgtggat acatacaaga aggagaatta ccagagggat ttatatgtcc agtctgtaag
     2341 agtccagcat cagtatttgt aaaagttgaa gaaaaaacag aggtagatga taaagaggat
     2401 aataaatcaa aatcaactaa tgaaagtagt aataaatata aaggaacaaa aactgaaaaa
     2461 aatttaatgg atgcattagc tggagaatca atagctagga ataaatatac attctttgcg
     2521 gatgtagcta aaaatgaagg atatgaacaa atatataaac tatttttaaa aacagcagga
     2581 aatgaaagag agcatgctaa attatggttt aaagaattag gatatttagg ggatactcaa
     2641 gagaatttat tgcacggatc agaaggagag cattatgaat ggtcagatat gtatgctaga
     2701 tttgcaaaag aggctgaaga agagggattt tttgatttag cagaaaaatt tgtggaggtt
     2761 gctaaaatag aaaaatctca tgaagataga tatagaaagc ttttaaacaa tataaagatg
     2821 aaaaaggtat ttgaaaaatc agaagaaact atgtgggagt gcttaaattg tggatatctt
     2881 gttatgggta aaaaagctcc agaggtttgt ccagtatgta attatgctga aggatttttt
     2941 gaagtaagag cagaaaatta ttaataataa tttagttata gattatagaa gtaagtttta
     3001 tgaattattt aaaattaatt ggcagtaaaa aataatttaa taaaaatgca aatagataaa
     3061 gagggtatga aaaaaattac cctctttatt taatagtctt attttatttg tgatgttttg
     3121 gtgaaatttc aactgttata ctaataccaa ttcctgctat tattccagtt aatatgtagt
     3181 aagttaagtt attcattggt attacatata aaccaagtag ccctattata agtaaaagta
     3241 aaaacataca taccacaaaa aatagtggtt tgaatttagg ggttgtggga ataatttttg
     3301 atattttatc tgataacttt aaacttttat ttataggtat aaaaataaaa gttgtgattg
     3361 ctagtgataa aatcattgga ataaaactca ttaaagattt catcttaagt tcctgctact
     3421 caaaatatgg tcaaatatat ccatatggag attattagat act
//
LOCUS       DAEVGI010000196         3398 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEVGI010000196 DAEVGI010000000
VERSION     DAEVGI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.198"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(196..735)
                     /locus_tag="K8M77_002356"
     CDS             complement(196..735)
                     /locus_tag="K8M77_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBI7086809.1"
                     /translation="MNLKGSKTEKNLLTAFAGESQARNKYTFYAEKARREGYNKIADI
                     FEETAHNEMAHAKIWFKFLNDGTVGETLDNLKDAAGGENYEWTDMYAEFAKEAREEGF
                     NEIAFILEKVGEIEKHHEERYLELYNLLKNGEIFKKDEKQLWRCQNCGFILESTVAPE
                     KCPVCHHPKAYFELVCDKF"
     gene            complement(953..1870)
                     /locus_tag="K8M77_002357"
     CDS             complement(953..1870)
                     /locus_tag="K8M77_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086810.1"
                     /translation="MKSKKLIVYSLIGIVLFSIVIFIGFKLKDESNKKTSNFEKILEV
                     NRKLEKENELSGGFPYTLEFLDYNITINSVKVFEDYALFDYSVKKIDGGYINTTDVNI
                     TGNVNFKGREFYISSNDVERRWEYSSVRIISTGEIIAKENKDSPANLSFSINARSADG
                     SYISKSVSIKEEVYLTDEDYLSLNKKITFDNVDFEIRSLSNFEFGSLLYIFVGNKDEE
                     ECKRIANQYSVKLISGDYERSFDLEKWVGFTELAIRKYSSIDERYNKDSKHDQFYMSR
                     MFYNLNEVNREDMEVYLINNRTKEEIRII"
     gene            complement(2035..3177)
                     /locus_tag="K8M77_002358"
     CDS             complement(2035..3177)
                     /locus_tag="K8M77_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBI7086811.1"
                     /translation="MNCNKTIYTPGPTNLRDNVRRARALKTTNPDVDVDFVEFYKETC
                     DRFGEIINTKNDCYILSGEGILGLEAACASLTEKGDKVLVIDNGLYGRWFDGFVTMYG
                     GEVTYFSGDYTKEIDVEALSKFLEKNNDFKYATVVHCDTPTGVLNPVDKICPLLKSYG
                     ILTVVDSVSAMVGEEFKVDKWQIDIALGGSQKAFSAEPGLTMVSISEDARKAMENREI
                     PIVGFYCNLNIWKNYYKDKWFPYTMPTSDIISLRVAIDNILEEGKEKVIERHRKIGEA
                     TRKAVKEYGLELFLKNGYSNTVTAVEIPEEIGALNLRNHLNKKYNVLMSTSLDRYENK
                     LLRIGHMGENANIEDITYVLNAIDKALKDLGFSSEKQLDKLFIENI"
BASE COUNT         1158 a          614 c          298 g         1328 t
ORIGIN      
        1 ttttatattt tattctagaa ataaaaaagg tataaaataa ataagaccaa ggataggaag
       61 aaatcttaaa acctaaacct tgatctcaat ttatttaaaa aggattaaaa taaatctttt
      121 ataggtaata tgctaatttg cggaagaagt taattctata aaacaatata atttaatcct
      181 tattaaatac tataattaga atttatcgca aactagttca aagtatgctt ttggatgatg
      241 acaaacaggg cacttttctg gtgctactgt tgattctaaa ataaatccac agttttgaca
      301 tctccataat tgtttttcat cttttttaaa tatttcacca ttctttaata gattatataa
      361 ttctaaatat ctttcttcat ggtgcttttc tatttctcct actttttcta atatgaatgc
      421 tatttcatta aaaccttctt ctcttgcttc cttagcaaac tcagcataca tatctgtcca
      481 ttcatagttt tctccaccag ctgcatcctt tagattatct aaagtttctc caactgtacc
      541 atcatttaaa aacttaaacc aaatctttgc atgagccatc tcattatgag cagtttcttc
      601 aaagatatca gctatcttat tatatccttc tcttctagct ttctctgcat agaaagtata
      661 tttatttcta gcttgtgact ctccagcaaa ggctgttaaa agattctttt ctgttttact
      721 tccttttaaa ttcatgaact ccacctcaaa atataatttt atagtctatc gatattattt
      781 ataataaaca tcaatacaat ttaatttttc cctttttgag gttttatatt ctaaatttaa
      841 agctaaaatt tttagctaaa ataaaacaaa gttctcatta ataaatacat aataaagttc
      901 aataaaaaaa tctataaata aacctcataa ataatttcaa tttattaagc ttttatataa
      961 tacgtatttc ctctttagtt ctattattaa ttaaataaac ttccatatcc tctctattaa
     1021 cttcatttaa gttatagaac attcttgaca tataaaactg atcatgcttt gaatccttat
     1081 tatatctttc atcaatacta ctatattttc ttatggctaa ctcagtaaat ccaacccatt
     1141 tttctaaatc aaaacttctt tcataatctc cacttattaa ctttacggaa tattgatttg
     1201 caattctttt acattcctct tcatccttat taccaacaaa aatatacagt aatgaaccaa
     1261 attcaaaatt tgataaacta cgaatttcaa aatcaacatt atcaaaggta attttcttat
     1321 ttaaacttaa ataatcttca tctgttaaat aaacttcttc ttttatggat acagactttg
     1381 atatataact accatcagct gatctagcat taattgaaaa gcttaaattt gcaggcgaat
     1441 ccttattttc tttggcaatt atttctccag ttgaaataat acgtacacta gaatattccc
     1501 atcttctttc aacatcattt gaagatatat aaaattctct ccctttaaag ttcacatttc
     1561 cagtaatatt aacatctgta gtgtttatat atcctccatc aatctttttc actgaataat
     1621 caaataatgc atagtcttca aatactttta ctgaatttat ggttatgttg taatctaaaa
     1681 actctagtgt gtatggaaaa cctccactta attcattttc tttttctaac tttctattta
     1741 cttctaaaat tttttcaaag ttacttgttt ttttatttga ttcatctttt aacttaaatc
     1801 caataaaaat gactatagaa aataaaacta ttccaattaa tgagtacact atcaatttct
     1861 ttgatttcat tgaaaactct ccttactatt tcattttcta cataatatca tttttttata
     1921 tcgtaacttt aaatccactg ttaaatttat acaaacaaaa aaaatgctta ataaaggtat
     1981 aatatttcct ttattaagca cttaaataca aattaattat tttagtaaaa cctattaaat
     2041 attttctata aataatttat ctaattgttt ttcacttgaa aatcctaaat cttttaaagc
     2101 cttgtctata gcatttagga cataggttat atcttctata tttgcatttt ctcccatgtg
     2161 tccaattctt aatagcttgt tttcatatct atctaaagat gttgacatta gtacattata
     2221 ttttttattt aggtgatttc ttaagtttaa agcacctatt tcttctggta tttccacagc
     2281 tgtaactgta ttagaatatc cattttttaa gaataactct aacccatatt cttttactgc
     2341 ctttctagtt gcttctccaa tttttctatg tctttcaata actttttcct ttccttcctc
     2401 tagaatgtta tctatagcaa ccctaagaga aataatatca cttgttggca ttgtatatgg
     2461 gaaccattta tctttgtaat aatttttcca tatattcaaa ttgcaataaa aacctacaat
     2521 aggtatttct ctattttcca tagcctttct tgcatcctca cttatactaa ccatagtaag
     2581 ccctggttct gctgaaaagg ccttttgaga tccacctagg gctatatcaa tttgccattt
     2641 atccacctta aattcctctc ctaccatagc agagacagaa tcaacaactg tcaaaattcc
     2701 ataactctta agtaatggac aaattttatc aacaggattt aatacccccg ttggagtatc
     2761 acaatgaaca actgtggcat atttaaaatc attattcttc tctaaaaact tagataaagc
     2821 ttcaacatca atctctttag tatagtctcc tgaaaaataa gttacttctc ccccatacat
     2881 ggttacaaaa ccatcaaacc atcttccata taaaccatta tcaataacta ataccttatc
     2941 tcctttttca gttaaggatg cacaggcagc ttctaaccct aaaattcctt ctccagaaag
     3001 tatatagcaa tcatttttag tatttataat ctctccaaac ctatcacaag tttccttata
     3061 aaactcaaca aaatccacat caacatctgg atttgtagtt tttaaagccc ttgctcttct
     3121 tacattatct cttaaattag taggacctgg tgtataaata gttttattac aattcataat
     3181 tcattcctcc aaacactctc atttttctct tttataaaaa aataacacaa ctattaaaaa
     3241 taaccaatag aatcgataca atataaaatt taaaatggat gtatataaaa gtaactattc
     3301 aataaaattc aaaagtaaaa agttttataa attttgatat ttacctttac actaaactct
     3361 tgatttcatg tttttatgtc tttggcaaga acacccgt
//
LOCUS       DAEVGI010000197         3334 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEVGI010000197 DAEVGI010000000
VERSION     DAEVGI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.199"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            281..985
                     /locus_tag="K8M77_002359"
     CDS             281..985
                     /locus_tag="K8M77_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBI7086812.1"
                     /translation="MASLLAELIGTMLLIILGDGVVANVVLKKTKGNSSGWIVITTGW
                     ALAVAIPAAIFTSVSGALFNPALTIALAIVGQFTWSQVIPYIIAQLLGAFLGAVVVYI
                     TYYNHFEETEDQNSKLGVFCTIPEIEDHKINFLTEFIGTFVLTFAVLGIGAQNIDYGV
                     KVIIIGCLIWAIGLSLGGPTGYAINPARDFGPRLAHFLLPIPRKGSSKWEYAWIPIVA
                     PIIGAICGALVYVNIF"
     gene            1172..2404
                     /locus_tag="K8M77_002360"
     CDS             1172..2404
                     /locus_tag="K8M77_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBI7086813.1"
                     /translation="MANLLDIREILDVNSFQKIQDDIAKATEFAMITVDYKGIPVTRH
                     SRCSEFCRLIREQKEFSKLCEKCDSRGGLEAAREGSFYIYKCHRGLVDVAVPIIIDGQ
                     YLGAVMVGQVLLTDEDDHELEAVLTKELEYDSQIKDKIIDAYEKIPVLSFDKIKAVSE
                     MMSHVSNYIVEEALLKRTQNEIYTKNIEIARAERIKLELEEEYKACQLKALQSQINPH
                     FLFNVLNSIASLAIIEDAPKTQEVIYNLSYILRYTLKKANKIVRLSEEINHVKAYLEI
                     QKVRFGERIQYNIDFDEEDSNVQIPFMALQVFVENAVLHGIEEKEQGGVINLSIKSKG
                     ENLVITIFDDGVGIPVEKICEIRNEIKSRDKLDLDKVGINNVNKRMFHYYGEEYSINI
                     DSKVKKGTKVEIIIPRKL"
     gene            2424..3176
                     /locus_tag="K8M77_002361"
     CDS             2424..3176
                     /locus_tag="K8M77_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBI7086814.1"
                     /translation="MFSIMVADDEQLERRVLTSILKKNARIKEIIEAKNGKEALELNR
                     EFNPDIIIMDIKMPGINGIKVLELIKNENPNKEIIMLTAYDDFEFIHKVLVLGGADYI
                     IKPIKPNKLMEIIDNIMNKIENKNIESEVKNYIDENTLDRNLKNEEKIVDKVSKFIDD
                     NMDKMLKLEELASICNLSPGYFSRVFKKETGKTVITYINEKKVDKAKKLLKESKDPII
                     NISLDLGFDDCGYFIRVFKKITGLTPKAFREE"
BASE COUNT         1373 a          297 c          585 g         1079 t
ORIGIN      
        1 tagaaaataa gttaaatgag attttagaaa gtttaaaaga aaaagaacat taaaatgttg
       61 aaaatttaac taatttaaaa tatttacatg gataaatttt cttgtgatag aaagtatttt
      121 tagtaaaatt aagaaattta ttaaaaataa aagaaatttt aaattataat cttgactatt
      181 ttttaacata tgtattatag tataattgtt gttaaaagga tatcaaatgt ttattaaaag
      241 gaattgtatt tgtgtcataa atatagaggg agtataattt atggcatcat tattagcaga
      301 attaatagga acaatgctac ttatcatact tggtgatggt gttgttgcaa acgtagtttt
      361 aaagaaaaca aagggaaata gctcaggatg gatagttata actactggat gggctttagc
      421 agttgctata ccagcagcta tttttactag tgtaagtgga gcattattta atccagcatt
      481 aaccatagca ttagctatag ttggccaatt tacatggtct caagttattc cttatataat
      541 tgcacaactt ttaggagctt ttttaggagc agttgttgtt tatataactt actataatca
      601 ttttgaagaa acagaagatc aaaattcaaa attaggagta ttttgtacta ttccagaaat
      661 agaagatcat aaaataaact tcttaacaga gtttatagga acttttgttt taacttttgc
      721 tgtattagga ataggagcac aaaatataga ttatggtgtt aaagtaatta taataggatg
      781 cttaatatgg gctataggat taagcttagg tggtccaaca ggatatgcaa taaatccagc
      841 tagagatttt ggtccaagac tagcacactt cttacttccg attcctagaa aaggaagttc
      901 taagtgggaa tatgcttgga ttcctatagt tgcaccaata ataggagcaa tatgtggagc
      961 cttagtttat gtaaatatat tttaataaac caaggtaaat aagtttaaaa ttattatcta
     1021 tagatcaata tagataataa ttttttttaa ttataaatga taaatttctt ataactttaa
     1081 aaatacgcta tttaatatta tgtttttaag tattattttt ttatagtaaa atatagtgtg
     1141 tatattatta aagctcttaa gaggaggaaa tatggcaaat ttattagata ttagagagat
     1201 tttagatgtt aattcctttc aaaaaataca agatgatata gctaaagcaa ctgagtttgc
     1261 tatgattaca gtagattaca aaggaatacc agttactagg catagtagat gtagtgagtt
     1321 ttgtagatta ataagagaac aaaaagagtt ctcgaaatta tgtgaaaaat gtgattcaag
     1381 aggtggatta gaagctgcta gagagggaag tttttacata tataaatgtc atagagggct
     1441 tgtggatgtg gctgttccaa taataattga tggacaatat ttaggagcag ttatggtagg
     1501 acaagttttg ttaactgatg aagatgatca tgaattagaa gctgttttaa ctaaggagtt
     1561 agaatatgat tcacaaataa aggataaaat tattgatgca tatgaaaaga taccagttct
     1621 tagctttgat aaaataaaag ctgtttcaga gatgatgtcc catgtaagca attacattgt
     1681 tgaggaagcc ttacttaaaa gaactcaaaa tgagatttat acaaaaaata tagaaattgc
     1741 aagagcagag agaatcaaat tagaacttga agaggaatac aaagcttgcc agctaaaagc
     1801 tcttcaatct caaataaatc cacatttttt atttaatgta ttaaatagca tagcatcttt
     1861 agcaattata gaggatgccc ctaaaactca agaagtaatt tataatttgt cgtatatttt
     1921 aaggtatact ttaaagaaag ctaataaaat tgtaagactt agtgaagaaa taaatcatgt
     1981 taaggcatat ttagaaattc aaaaggtccg ttttggtgaa agaatacaat ataatataga
     2041 ttttgatgaa gaggattcaa atgtacaaat tccatttatg gcacttcaag tttttgttga
     2101 aaatgcagtt ttgcatggaa ttgaagaaaa agaacaggga ggagtcataa atctatcaat
     2161 taagagtaag ggagagaatc tggttattac tatttttgat gatggtgttg gaataccagt
     2221 tgaaaaaatc tgtgaaatta ggaatgaaat aaaaagtaga gataaactag acttagacaa
     2281 agttggaata aataatgtaa ataagagaat gtttcattat tatggtgaag aatatagtat
     2341 aaatatagat agtaaagtta aaaaaggtac taaagtagaa attataatac ctagaaaact
     2401 ttaaataaag gaagtgtatt ttggtgttta gcataatggt tgctgatgat gaacaattag
     2461 aaagaagagt tttaacctct atattaaaaa aaaatgcaag aattaaagaa attattgaag
     2521 cgaaaaatgg aaaagaagcc ttagaattaa atagagagtt taatccagat ataataataa
     2581 tggatataaa aatgccagga ataaatggta ttaaagtctt ggagcttata aaaaatgaaa
     2641 atcctaataa ggaaataata atgcttactg catatgatga ttttgagttt atacacaaag
     2701 ttttagtatt aggaggagct gactacataa taaaaccaat aaaaccaaat aaattgatgg
     2761 aaattattga taatattatg aataagattg aaaataaaaa tatagaatca gaagtaaaaa
     2821 attatattga tgaaaatact ctagacagaa acttaaaaaa tgaagaaaaa atagtagata
     2881 aagtttctaa atttattgat gataatatgg ataaaatgct aaagttagaa gaattagctt
     2941 cgatctgtaa tttgagtcca gggtatttta gtagggtttt taaaaaggaa actggtaaaa
     3001 ctgtaataac atatataaat gagaaaaagg tagacaaagc aaagaaactt ttaaaagaaa
     3061 gtaaagaccc tataatcaat atatccttag acttggggtt tgatgattgt ggttatttta
     3121 taagggtatt taaaaagata acaggcttaa ctcctaaggc ttttagagaa gaatgattaa
     3181 ttattaaatt aacaaacata agatttttgt gagaaatgtt acagatgaaa aaaataatat
     3241 aagataatat attgttataa ataaagcaac ttttatagga aaatatagtt ataagtaaat
     3301 aaatatatta aaaaaatgac atacaaatag gatt
//
LOCUS       DAEVGI010000198         3303 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEVGI010000198 DAEVGI010000000
VERSION     DAEVGI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.200"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..1093)
                     /gene="fliB"
                     /locus_tag="K8M77_002362"
     CDS             complement(<1..1093)
                     /gene="fliB"
                     /locus_tag="K8M77_002362"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HBI7086815.1"
                     /translation="MKTNFFIPKYMKTFKCIGPNCTDTCCAGWDINIDENTFKKYEND
                     KGNLKELIKGKYIKNSESDDSFNYGFMKITEDNKCPFLNENLLCEIHGKCGEENLSIT
                     CRRYPRIFNIIDDIYEKSGLPSCEEICSKAFLNKEKMEFIEIEEELPEDFLEIRRVID
                     TEAFIGSDNLIQYFWDIRIISINIMQNRNFSIEERLSLLKAFYKSLEDLKTEENFYEI
                     EDLLEKITEYPSNITQFIDSKKIIPLSITNNFFKIILDENLLRKVIGTRLKNLLSDLN
                     KDQNLFNNIHKYDLKSFDTYFKKYSYIFENYLVNQIFKDIIPFNTGGDLNESIHKLIN
                     TYKLIKSYLILWNISSQNEISEKNIIYVIQ"
     gene            1332..2345
                     /locus_tag="K8M77_002363"
     CDS             1332..2345
                     /locus_tag="K8M77_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7086816.1"
                     /translation="MNKVYLKQKDETGKRSSKLKKWICGILVAIIICIILALGFGGNY
                     LYNLAINPDTPKDIVFESTESSKDVVTINSTSGPVSISSEEWLLKESGYEDLYMTSRD
                     GLKLHNYLIKKPNSNKWVITVHGYTSQGKLTSYYAKNFSDMGYNVIIPDLRGHGTSEG
                     DYIGMGWDERLDIIDLINYIIKEDKEAEIVLYGISMGAATVLNTSGEELPENVKALVV
                     DCGYTSAWDEFSYQLNKLFGLPAFPMMHIANLITKIRAGYWINESSPIDQTAKSKTPT
                     LFIQGDEDTFVPAFMVEELYNASSAEKEKLIVKGAGHAKASKVNPKLYWETIDGFLNK
                     YVS"
     gene            complement(2509..3153)
                     /locus_tag="K8M77_002364"
     CDS             complement(2509..3153)
                     /locus_tag="K8M77_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7086817.1"
                     /translation="MNLISNFFDLILHLDKYLGVFINNYGTLTYLVLFLIILAETGLV
                     VTPFLPGDSVIFASATFCALGMINIYILVPLLLIAAILGDTLNYSIGKFLGSKLLANS
                     KLIKKEYIEKTNTYYDKYGGKTLIIARFIPIIRTFAPFVAGIGTMKYKNFLTYNAVGG
                     FIWVILVSSLGYFFGNIKIVAENFSIVIIGIIVVSILPAIVGILKAKFSPKATM"
BASE COUNT         1257 a          399 c          441 g         1206 t
ORIGIN      
        1 cttgaattac atagattata tttttttctg aaatttcatt ttgtgatgag atgttccaaa
       61 gtattaaata gctttttatt agtttataag tatttataag cttatgaata ctttcattta
      121 aatcaccacc tgtattaaag ggaattatat ccttaaaaat ttgatttact aagtaatttt
      181 caaatatgta tgagtacttc ttaaagtaag tatcaaagga ttttaagtca tatttatgta
      241 tattgttaaa taaattttga tccttattta aatctgataa caaattcttt agcctagtac
      301 caatgacttt tcttaaaaga ttttcatcta agataatctt aaaaaagtta ttggtaatac
      361 taagtggaat aattttttta gaatcaataa attgggttat atttgatgga tattcagtta
      421 ttttttcaag taaatcttca atttcataaa agttttcttc agtttttaga tcttctagac
      481 ttttataaaa ggcttttaat agacttagtc tctcctcaat agagaaattt ctgttttgca
      541 taatatttat tgatataatt cttatatccc aaaaatattg aattaaatta tctgaaccta
      601 taaaagcttc agtatctatt actctgcgta tttctaggaa atcttcagga agttcttctt
      661 ctatttctat aaattccatt ttttctttat ttaaaaaagc tttagaacaa atttcttcac
      721 aggaaggaag tccgcttttc tcatatatat catctataat attaaaaatt ctaggatatc
      781 ttctacaggt tatagataag ttttcttcgc cacatttacc gtgaatttca caaagtaagt
      841 tttcatttaa aaaggggcat ttattatctt ctgttatttt cataaatcca taattaaatg
      901 aatcatctga ttcagagttt tttatgtatt ttccctttat taattctttt agatttcctt
      961 tatcattttc atacttttta aaggtatttt catctatgtt aatgtcccaa ccagcacaac
     1021 aagtatcagt gcaattagga cctatgcatt taaatgtctt catatatttt ggaataaaaa
     1081 aatttgtttt caaaacaaca ccatctttcg tgtaaaatta tactgcctta atattttatc
     1141 actaagtttt ataaatagtt agtaattttt ttagaaagta aaattaatga aaaagtatat
     1201 ttttattaca tcaagtacat ttgattttta atattttaat aatagaatta tttgctataa
     1261 taaatatttg atagtcaaaa taattcgtga tataataatg acttaaaaaa ttcttttata
     1321 aaggagagca tatgaataag gtatatttaa aacaaaaaga tgaaacgggt aaaagatcga
     1381 gtaagcttaa aaaatggatt tgtggaatat tagtagcaat aataatatgt attattttag
     1441 ctttagggtt tggaggaaac tatttatata atttagctat aaatcccgat acaccaaagg
     1501 atatagtctt tgaatcaact gaaagtagta aagatgtggt tacaataaat agtacaagtg
     1561 gaccagtaag tatttcaagt gaagaatggc ttttaaagga aagtggttat gaagatttat
     1621 atatgacttc aagagatggg ctaaaattac acaattatct tataaaaaaa ccaaactcta
     1681 ataagtgggt tattacagta catggctata catctcaagg aaaattaaca tcatactatg
     1741 caaaaaactt ttctgacatg ggatataatg ttataatccc tgatttaaga ggtcatggca
     1801 ctagtgaagg tgattatata ggaatgggat gggacgagcg tttagatata atagatttaa
     1861 ttaattatat aataaaagaa gataaagagg cagagattgt tttatatggt atatcaatgg
     1921 gggcagctac agttcttaat acatcaggag aagaacttcc agagaatgtt aaagcccttg
     1981 tagtagactg tggatatact agtgcatggg atgaattttc ttatcagtta aataaacttt
     2041 ttggtcttcc agctttccct atgatgcata tagcaaattt aataacaaaa attagagctg
     2101 gctattggat aaatgaatcg tcacctattg atcaaactgc aaaatcaaaa acaccaacat
     2161 tatttattca aggagatgaa gacacctttg ttccagcctt tatggtagaa gaactttata
     2221 atgcctcttc tgctgaaaag gaaaaattaa tagtaaaagg agctggacat gctaaggcaa
     2281 gtaaggttaa tccaaaactt tattgggaaa caatagatgg ttttttaaat aaatatgtta
     2341 gttagtttta agatataaaa taaactataa ataaagagag aataaataat acaagcaaaa
     2401 aagctttgaa aaatttaata gatcggatta aataatatct aaaaaaacaa acagtaatat
     2461 tttattgctg tttgtttttt atcttaaata tatgatttta aatttttatt acatagtagc
     2521 tttaggtgag aatttagctt taagtattcc tactatagca ggtaagatag aaacaactat
     2581 tatacctata ataactattg agaaattttc agcaacaatt tttatattcc caaagaaata
     2641 tcctaaactt gatacaagta taacccaaat aaatccacca actgcattgt aagttaaaaa
     2701 gtttttatac ttcattgttc ctatacctgc aacaaagggt gcaaaggtac ggattatagg
     2761 aataaatcta gctattatta aggtttttcc accatattta tcataataag tattagtttt
     2821 ctcaatatac tcttttttta taagcttaga attagcaagt aatttgctac ctaaaaattt
     2881 accaatacta tagtttaaag tatctccaag tatggctgca attaataata atggtactaa
     2941 tatgtaaata ttaatcatac ctaaagcgca gaaagtagct gatgcaaata ttactgaatc
     3001 tccaggaaga aaaggagtta ctactaatcc tgtttccgca aggattatta aaaataaaac
     3061 taagtaagtc aaagttccat aattattgat aaaaacacct agatatttat ctaaatgtaa
     3121 tattagatca aaaaaattac ttatcaagtt cataaaaaat ctcctaacta aaatgaataa
     3181 atctatcttt tattatatta ctaattcaaa tattattcat taatttttga ataaaatgtt
     3241 aattaaagta taaaataata ataaagaaat agaaatatga tttaaatatt atataaactt
     3301 ttt
//
LOCUS       DAEVGI010000199         3297 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEVGI010000199 DAEVGI010000000
VERSION     DAEVGI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.201"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1076
                     /locus_tag="K8M77_002365"
     CDS             <1..1076
                     /locus_tag="K8M77_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBI7086818.1"
                     /translation="LIEKRKVLENLETKSLIEKEEPSDLYGILRGFLEKVDIYESKSS
                     EENSNLLELYFNVYEFLNICSYYGEDFTTLYKLDGNNLELSINCLDPSRILKSIMNRF
                     KSVIIFSATLLPMEYFKELYGAQEGDYSVNLTSPFDYKNKLTIVGKDVSTTYSNRKRT
                     LPKIVNYIIECVKSKRGNYLVFFPSYEYMWMVHEEIKKREVDFSLVAQGDHMNEEEKE
                     EFLSLFREGGEKTHLGLAVLGGHFSEGIDLTLDKLIGVIIIGVGMPKICLERESIKEY
                     YNSIGKNGFDYAYVYPGIIKVLQAAGRCIRTEKDKGVVLLLDDRYFTNKYKSLLPREW
                     FLNILVESEEGIKNTCENFWKEV"
     gene            1266..3254
                     /locus_tag="K8M77_002366"
     CDS             1266..3254
                     /locus_tag="K8M77_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBI7086819.1"
                     /translation="MSENKFLPICKDDMIERGWEQCDFVLVTADAYIDHHSFGTAIIS
                     RVLENAGYKVGIIAQPDWKSVDDFKKLGRPRLGFLVNGGNMDPMVNHYTVSKKLRKKD
                     LYTPKGEMGKRPDRATIVYCNKIREAYKDVNIVIGGIEASLRRFAHYDYWENKVRKSI
                     LVDSGADLLVYGMSEKQIVEVADFLNQGFDGKYIRHIPGTCYIADSLDEIYEEHIVLP
                     SFKDVSSDKRTYAECFKIQYDEQDPVRGRTLVQEHNGKYVVINKPEMPLSREELDRVY
                     ALPYQKTYHPIYEKDGGIAAIEEVKFSLVSSRGCSGNCSFCAITFHQGRIVTSRSEDS
                     IVEEAEEITKYDDFKGYIHDIGGPTANFRKPACKKQLTLGACKHKRCMSPGICKNMEV
                     DHREYLHLLRRVRKLPGIKKVFIRSGLRYDYIMADKDDTFFKELVEHHVSGQLKVAPE
                     HVSPNVLKYMGKPAGKTYDEFRRKFFKITERLGKKQFIIPYLMSSHPGCKLEDAIMLA
                     EYLRDINYQPEQVQDFYPTPGTLSTTMFYTGLDPLTMEEVYIPRSKEEKAMQRALLQF
                     KNPKNYNIVYDALVKAGREDLIGNGPKCLIRDKNSFGKGNNHSNHKSGGRKSRNENSG
                     RRESEDKKRSSHSKKQRGNKSRGFDQKSQRVSKGKKRR"
BASE COUNT         1311 a          342 c          652 g          992 t
ORIGIN      
        1 atttaataga aaaaagaaag gttttagaaa atttagaaac taaaagtctt atagaaaaag
       61 aagaaccaag tgatttatat ggaattttaa gaggcttttt agaaaaggta gatatttatg
      121 agagtaaatc tagtgaagag aattcaaatc ttttagagtt atattttaat gtttatgaat
      181 tcttaaatat atgttcttat tatggagaag attttactac tttatataaa ttggatggaa
      241 ataacttgga attatcaatc aactgtttag atccatctag gatacttaaa agtataatga
      301 ataggtttaa atcagttata atattttcag ctactcttct tcctatggag tattttaaag
      361 aactttatgg agcacaggaa ggggattatt ctgttaattt aacttctccc tttgattata
      421 aaaataaact tactatagtt ggaaaggatg tatcaacaac ctattctaat agaaagagaa
      481 ctcttcctaa aatagttaat tacataatag aatgtgttaa gagcaagaga ggcaactatc
      541 ttgtattttt cccttcttat gaatatatgt ggatggttca tgaggaaatt aaaaaaagag
      601 aagttgattt tagtttagta gctcaagggg accacatgaa tgaagaggaa aaagaagagt
      661 ttttatcttt atttagagaa ggaggagaaa aaacacatct tggcttagct gttttaggtg
      721 ggcatttttc agagggaata gatttaacct tagataaact tattggagtt ataattattg
      781 gagttggtat gcctaaaatt tgcttagaga gagaaagtat aaaagaatat tataactcta
      841 taggtaaaaa tggttttgac tacgcctatg tttatcctgg aataataaag gtgcttcaag
      901 cagcgggaag atgtataaga acagaaaaag ataagggggt agtactttta ttagatgata
      961 gatactttac aaataaatat aagagtctac ttccaagaga atggttttta aatattttag
     1021 tagaaagtga agaaggaatt aaaaacactt gcgaaaattt ttggaaggaa gtttaaagag
     1081 gttgcaaaaa atataataat aaacatattg agaaaataat atatttctct agaaatttta
     1141 tttacactat ttataaggtt tactaaaaat tattttgata cagactcttt ttaatgttat
     1201 aatcatatga agatttattt agaggagttt taaataagct taagaagaga caagtaggtg
     1261 aagaaatgag tgaaaataag tttttgccaa tttgcaaaga tgatatgata gaaagaggat
     1321 gggaacaatg cgactttgta ctagttacag cagatgcata catagaccat catagttttg
     1381 gtacagcaat tatatctagg gttttagaaa atgctggata taaggttgga ataatagctc
     1441 aaccagattg gaagagcgtt gatgatttta aaaaattagg tagaccaaga ttaggattct
     1501 tagttaatgg tggtaatatg gatcctatgg ttaatcacta tacagtaagc aaaaagttaa
     1561 gaaagaaaga tctatatact cctaaggggg aaatgggtaa gagacctgat agagctacaa
     1621 tagtttattg taataaaata agagaagctt ataaggatgt taacatagta attggtggaa
     1681 ttgaagctag tttaagaaga tttgctcatt atgattactg ggaaaacaag gtgagaaaat
     1741 caatcttagt tgatagtgga gcggaccttt tagtatatgg aatgagtgaa aagcaaattg
     1801 ttgaagtggc tgacttttta aatcaaggat ttgatggaaa atacataaga catataccag
     1861 gaacatgtta catagccgat agtttagatg aaatctatga ggagcatata gttctaccat
     1921 catttaaaga tgtttcaagt gataagagaa cttatgcaga atgctttaaa attcaatatg
     1981 atgagcaaga tcctgtaaga ggaagaactt tagttcaaga acataatgga aaatatgttg
     2041 ttataaataa accagaaatg cctctttcaa gggaagaatt agatagagtg tatgctcttc
     2101 catatcaaaa aacttaccat cctatttatg aaaaagatgg tggtatagct gctattgaag
     2161 aggttaagtt tagtttagta agttcaaggg gatgctcagg aaactgttca ttctgtgcaa
     2221 taaccttcca ccaaggaaga attgtaacta gtagaagtga agattctata gtagaggaag
     2281 ctgaagaaat aactaaatat gatgatttta aaggatatat acacgacata gggggaccta
     2341 cagctaactt tagaaagcca gcatgtaaga agcaactaac tttaggagct tgtaaacata
     2401 aaagatgtat gtcaccaggt atatgtaaga acatggaggt agatcataga gaatatcttc
     2461 atttattaag aagagtaaga aaattaccag gaattaaaaa ggtatttata cgttcaggat
     2521 taagatatga ttatataatg gcagataagg atgatacttt ctttaaggaa ttagttgagc
     2581 atcatgtaag tggtcaatta aaagttgcac cagagcatgt atctccaaat gttttaaaat
     2641 acatgggtaa accagcagga aaaacttatg atgaatttag aagaaaattc tttaaaatta
     2701 cagaaagatt aggaaagaaa caattcatca ttccttattt aatgtcaagt catccaggat
     2761 gcaagttaga agatgcaatt atgcttgctg aatatttaag agatataaat tatcaaccag
     2821 agcaagtaca agatttctat ccaacaccag gaacattatc aactacaatg ttctatactg
     2881 gattagatcc tttaacaatg gaagaagtat atattcctag aagtaaagaa gaaaaagcaa
     2941 tgcaaagggc tttattacaa tttaaaaatc caaagaatta taacatagtt tatgatgctt
     3001 tagttaaagc cggtagagaa gatttaattg gtaatggtcc aaaatgctta attagagata
     3061 agaatagctt tggaaaagga aataatcata gcaatcacaa aagtggtgga agaaagagta
     3121 gaaatgagaa tagtggaaga agagagtcag aagataagaa aagaagttct catagtaaaa
     3181 aacaaagagg aaacaaatca agaggatttg atcaaaagag ccaaagagtt tcaaaaggaa
     3241 agaaaagaag ataattaaat ataagaaagg tggttattga gtttttgctt aataacc
//
LOCUS       DAEVGI010000200         3261 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEVGI010000200 DAEVGI010000000
VERSION     DAEVGI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.202"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            138..2387
                     /locus_tag="K8M77_002367"
                     /pseudo
     CDS             138..2387
                     /locus_tag="K8M77_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
     gene            2778..>3261
                     /locus_tag="K8M77_002368"
     CDS             2778..>3261
                     /locus_tag="K8M77_002368"
                     /inference="COORDINATES: protein motif:HMM:NF019908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HBI7086820.1"
                     /translation="MSFEKGLGTHADSTIIYDISGKNYDYFEAYIGLDTETGESSDGA
                     IFKVLADNKEIYSSGVIKPKERARLIKLDIKGASKLTLKTLKSGHDWEDHTDWANAMF
                     TYKVKNLSEDLGLLIENSKEVYKNSIEGFNIREYHYGAKGELNNYIKLAEDVYKDNFS
                     S"
BASE COUNT         1272 a          304 c          652 g         1033 t
ORIGIN      
        1 gtttttttat attactcttt aacataatct atttttaaaa taaaatcaaa aaaataaata
       61 gtctttaata tttattgtaa taaatattat gttatttgaa taaacatata taggataaat
      121 atagttaagg aggaagtatg aaagttaaag ctataaaaag ttttttaata actacaatgg
      181 caatgacttt tttagttagt atgggacaag gatctatatt aactaaggca aatactataa
      241 ataatatgga atatgtaaca gaagtatttg gagcaattcc aacagaagaa caggttagct
      301 atcaaaaaga ggaacttaca gcatttatac actttggtgt gaataccttt acgggaaggg
      361 agtggggaga tggaacagaa aatccagaaa tttttaaccc tactaattta gatgctgatc
      421 aatgggtaaa aactttggcg gaggcaggct ttggaagggt tatattaact gcaaaacatc
      481 atgatggatt ttgtttgtgg gatagtgcat atacaaagca tgatgtagct agtagtcctt
      541 ggaaaaatgg aaagggagat gtggttaaag aggttttaga agcttgtgct aaatataata
      601 taaaattcgg agtttatttg tcaccatggg accaaaattc agaacattat ggagacggca
      661 atggtggtga ttataatgag ttttacatga atcagttaag ggaattatta actaactatg
      721 gacctatagc tgaagtttgg atggatggag caaagggatc gaatgttaag caagaatata
      781 actttgaaga atggtttgct cttataaaaa aattacaacc agaatgttta atatttagtc
      841 cacaggggcc agatattaga tggattggaa atgaaaaagg atatgcagga gaaccttgct
      901 ggtcaactat agatattgaa aaaatgaaag aaagggaaaa tccaacatat ttgaataatg
      961 gagaagaagg tggaccaaat tggatagttg gagaatcaga tgtatccata agaccaggtt
     1021 ggttttacca tgaatctcaa gataatgaag ttaaatcctt agaaaaaatg atggatattt
     1081 attttaaatc catagggaga aattcagttt tattattaaa tgttccccct aataaggaag
     1141 gaaagttaca tgaaaatgat gttaatagat taaaggaatt tggtgaaaca attaaagagt
     1201 tatttaatga tgatttagct ttaaataaag aggtaatagt agatggtttt gctaataatg
     1261 atgagacata tggtgcaaat aaaattgtag atggagatta tgatacctat tgggcaccag
     1321 ataatagtag taaaacaggt actattgaaa tagatttagg tggaagtaaa gaatttgatg
     1381 ttatttcttt gcaagagtat ataccattag gtcaaagagt atccagtttt aatgtagaag
     1441 tattgcaagg agaaaattgg aataaggttt atgaaggaaa aacaatagga tataaaagac
     1501 ttgttagaat agctccaact aaaggagaaa aaataagaat taatataacg ggttcattag
     1561 aagtaccact tataaataac gttggagttt ataaacaacc tattagtata gaacttccat
     1621 cagggccacc agctgggttg aaagtattaa atgatgataa taagggaaat gaattagaac
     1681 aatttaattt tagtgatgga tggatatatg agactatcca tggagaagat gatttaggtg
     1741 gagatgccca ttatacaagt aaaattaatg ctacagttaa tattaaattc aagggaatta
     1801 agtttttctt atcaggaaca aaggattcag gacatggaat aatggaaatt tcaatagatg
     1861 gtgaaaatcc agtagaggtt gacttatatt ctcctaatag aaaatctaaa gagatagttt
     1921 ttgaaagtga agatttaagt gatggagagc atgaagttac ggttaaatgt actggaagaa
     1981 agaattcaaa ttctagaggg atagcggctc atatagatgg agcttatgta ttagacaatg
     2041 gtggaaaagg tatggttgaa tttgaaaagg taggatacaa agtaagtgaa aatataggaa
     2101 ctgctacttt taaggttata agaaaaggag gaagtaatgg taagcttgaa gttaactatg
     2161 atactttagc tggcactgct ttaaatggag ttgattatca aacatggtct ggtactttag
     2221 catttaatga aggagaaaca gaaaaaactt ttgatataac aataatttat gataaggaaa
     2281 aagaagagcc taaggaattt tatttaaaat taagtgatcc aataggtgga atattaggat
     2341 ttaattcaag agctacagtt attattaatg atgatgagca aattaaataa taaattatat
     2401 attaacataa tttttatgtg ctatatctaa ttatataaaa attttaaaca tttaagtatg
     2461 ttagaaattt tttatgaaga tacagtttgt ttattacata tatttataat tgatgtgaaa
     2521 atacagtaat aaatataatt attacttgaa aattatggcg ttttatatta gttttattca
     2581 ttgctttttt ctcctttctt gaggagggat aaatgttttt cgcaaactca ttgtattctc
     2641 aaaagttgaa aagagcaatg ttcatttaac ttaacagatc gttccttcct taatggggga
     2701 taaatatttt ttgcaaattc attatattct aaaaggttga aaagagtaat gttcatttaa
     2761 ctgatggatt aaattatatg agttttgaaa agggattagg tacgcatgct gattcaacaa
     2821 taatttatga tatttcaggt aaaaattatg attattttga ggcttatatt ggattagaca
     2881 ctgaaactgg agaatccagt gatggagcta tatttaaagt tctagcagat aataaagaaa
     2941 tatatagtag tggggttata aaacctaaag aaagagctag acttataaaa ttagatataa
     3001 aaggagcatc taaattaacc ttaaaaacat tgaaaagtgg acatgattgg gaagatcaca
     3061 cagattgggc taatgcaatg ttcacatata aagtgaaaaa tttaagtgaa gatttaggcc
     3121 ttttaataga aaattctaaa gaggtttata agaattccat tgaaggtttt aatattagag
     3181 aatatcatta tggtgctaag ggagaattaa ataattatat taagttagcg gaagatgtct
     3241 ataaagataa cttttcttca a
//
LOCUS       DAEVGI010000201         3260 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEVGI010000201 DAEVGI010000000
VERSION     DAEVGI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.203"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            297..752
                     /gene="tnpA"
                     /locus_tag="K8M77_002369"
     CDS             297..752
                     /gene="tnpA"
                     /locus_tag="K8M77_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS200S family
                     transposase"
                     /protein_id="HBI7086821.1"
                     /translation="MDTNSLAHTKWNCKYHIVFAPKHRRKEIYGEKKQEISEILRQLC
                     EWKGVRIVEAHACVDHIHMLVEIPPKMSVSSFVWVLKCKSSLMIFEKFANLKYKYGNR
                     HFWCMGFYVDTVGKNKKAIEDYIRNQEQEDMIADQISLKEYMDTFKCSK"
     gene            1960..2919
                     /gene="cpe"
                     /locus_tag="K8M77_002370"
     CDS             1960..2919
                     /gene="cpe"
                     /locus_tag="K8M77_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin CPE"
                     /protein_id="HBI7086822.1"
                     /translation="MLSNNLNPMVFENAKEVFLISEDLKTPINITNSNSNLSDGLYVI
                     DKGDGWILGEPSVVSSQILNPNETGTFSQSLTKSKEVSINVNFSVGFTSEFIQASVEY
                     GFGITIGEQNTIERSVSTTAGPNEYVYYKVYATYRKYQAIRISHGNISDDGSIYKLTG
                     IWLSKTSADSLGNIDQGSLIETGERCVLTVPSTDIEKEILDLAAATERLNLTDALNSN
                     PAGNLYDWRSSNSYPWTQKLNLHLTITATGQKYRILASKIVDFNIYSNNFNNLVKLEQ
                     SLGDGVKDHYVDISLDAGQYVLVMKANSSYSGNYPYSILFQKF"
BASE COUNT         1243 a          376 c          446 g         1195 t
ORIGIN      
        1 attaaatcat aacacaggtt aatccaccat ggatttttta tttagttcaa tttcatttta
       61 caattaagcg tataataatt ttaagctatt actatatgtg aatagtcttt cctttatttg
      121 aaattaagaa aacaaagaaa gctctataaa ataagtaggg ctttcttgtt gtgtaatgaa
      181 aaccacttgc tatgcgagtg tgttcagaag agctttagcg atgtaagaaa aaacctcctt
      241 ttgttaaaat gaaaataggt tcgtagccaa ttttcgtaac aaaaggaggg ttttaaatgg
      301 atacaaacag tttagcacat acaaaatgga attgtaaata tcatatagtt tttgcaccaa
      361 aacacagaag gaaagaaata tatggagaga aaaaacaaga aataagtgag atattaaggc
      421 agctttgtga atggaaagga gttaggatag tcgaagcgca cgcttgcgta gatcacatac
      481 acatgcttgt tgaaatacca cctaaaatga gtgtttcaag tttcgtttgg gttctaaaat
      541 gtaaaagtag tcttatgatt tttgagaaat ttgcaaattt aaaatataaa tatggaaata
      601 gacatttctg gtgtatggga ttttacgttg atactgtcgg aaaaaataag aaggcaattg
      661 aagactatat aagaaatcaa gaacaagaag atatgatagc tgatcaaata agtttaaaag
      721 aatatatgga cacttttaag tgtagcaagt aaagaagtat gcggctggcg gactaattta
      781 agcgtcttaa gatgcaacgt aggtagtatg cccttatagg gctaaggaaa taccaccagc
      841 taagctggtg gatttttatt ttaacttagg cacttataca taaagtcttt taaatatttt
      901 aacttaaatt tctataaatg ttaattgtga aattattact aagataaata ttaatagtgt
      961 atacattgag atagtaaata gctatgatat ttaaattttt aaagtaattt taataaattt
     1021 atataattct gaaaatataa aaggtagctc attttagagc taccctaaat acgattagaa
     1081 atctttttat ttctatttta ttattagtta aaaatctata atttttattt atacatattt
     1141 tctacttttc acaataactg atgaaataga aactctaaat gaatcagata catcacatta
     1201 atgttcacca attcattttg tatacaatct attaacttga agaatggatt agtttatcaa
     1261 aactttaatt agagttatta ttatatttat taaaaactct ctcctttaaa ttaagttgat
     1321 atatatgaaa aatacatacg cagggttaga gataaaaata gtattcaagt ttataaaata
     1381 cttagagtat ctataaactt gatactcatt tattaataag ataaaattat ttatttttat
     1441 cttattaatc gctatattca ttatagttac ttgttgggtg atttattact ccaataagta
     1501 ccaaaattta gagtatagct ttaactattt cttgatagtt atctggtaaa atattaattt
     1561 taaatccttg taaaaccaag gaaattaagg ttgctataga aagccataac ccataattct
     1621 taaaacgtga tttatttatt ataatatatc actctccttg tttataatat ataatattat
     1681 gtttagtgaa attatgttaa tatactactt atttcttctt ttatattaat taacatttca
     1741 acttgatctc tttaacgtat atctctttta ttacccaagc tttaattcct tcagcattaa
     1801 tatcataaaa tgtccatgta gaaatatatt caagattatt aaagatatat attttattta
     1861 atattttgtt aatactttaa ggatatgtat ccaaaataaa aacttttaaa taatatatta
     1921 tataaaaaaa attagaaata aggagatgtt aattataata tgcttagtaa caatttaaat
     1981 ccaatggtgt tcgaaaatgc taaagaagta tttcttattt ctgaggattt aaaaacacca
     2041 attaatatta caaactctaa ctcaaattta agtgatggat tatatgtaat agataaagga
     2101 gatggttgga tattagggga accctcagta gtttcaagtc aaattcttaa tcctaatgaa
     2161 acaggtacct ttagccaatc attaactaaa tctaaagaag tatctataaa tgtaaatttt
     2221 tcagttggat ttacttctga atttatacaa gcatctgtag aatatggatt tggaataact
     2281 ataggagaac aaaatacaat agaaagatct gtatctacaa ctgctggtcc aaatgaatat
     2341 gtatattata aggtttatgc aacttataga aagtatcaag ctattagaat ttctcatggt
     2401 aatatctctg atgatggatc aatttataaa ttaacaggaa tatggcttag taaaacatct
     2461 gcagatagct taggaaatat tgatcaaggt tcattaattg aaactggtga aagatgtgtt
     2521 ttaacagttc catctacaga tatagaaaaa gaaatccttg atttagctgc tgctacagaa
     2581 agattaaatt taactgatgc attaaactca aatccagctg gtaatttata tgattggcgt
     2641 tcttctaact catacccttg gactcaaaag cttaatttac acttaacaat tacagctact
     2701 ggacaaaaat atagaatctt agctagcaaa attgttgatt ttaatattta ttcaaataat
     2761 tttaataatc tagtgaaatt agaacagtcc ttaggtgatg gagtaaaaga tcattatgtt
     2821 gatataagct tagatgctgg acaatatgtt cttgtaatga aagctaattc atcatatagt
     2881 ggaaattacc cttattcaat attatttcaa aaattttaat attttaaaat aatataatca
     2941 aattaattta caaaagacag tatgtaatat taaattatta catactgtct aattttttta
     3001 ttataattta atttttcata tatcacgata atatttttca gtttctttat ttttattttt
     3061 taaattttca atttctaata aaaatttatt ctgttcaaat ttactattat tcgatagttt
     3121 gactgtaaag taagtattat agcattaatt tctccttcca tatatttgag gtgttttttc
     3181 tattttaaaa acaactaatt aaaggattaa acaaatatgt attacaagta catatcattg
     3241 gtgatagtaa ataaatattc
//
LOCUS       DAEVGI010000202         3248 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEVGI010000202 DAEVGI010000000
VERSION     DAEVGI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.204"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..709
                     /locus_tag="K8M77_002371"
     CDS             <1..709
                     /locus_tag="K8M77_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086823.1"
                     /translation="GGASFNSANSSSGGRNGSSGVDFTFIMKHKLLFGSVSFFLLSGG
                     VVLMFVEMDRYSKVVKRKSDKLKELALKDPCWNINYIKSVVEDTFYSIQYAWNDDEYE
                     RVESLLTTSLYNKHLKVLEELKRENKKNVLKDIQFKGCRIISLFEGDEKQWGFIWVEV
                     NFSMIDYIVNTKGKRRIIEGNDNCSRSDFEYWKFVYIENTWLLSNILQKNEVGSGYKK
                     LYREAENAYGSINMKKV"
     gene            730..1023
                     /locus_tag="K8M77_002372"
     CDS             730..1023
                     /locus_tag="K8M77_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086824.1"
                     /translation="MTIDMGWMILGFGAFMFLIASVGQLRNEVKLMRWTLNRIAERVG
                     VTEIVTKEEKEELKRLLSEGKNVQAVRRCREITDLGLKEAKEYVDGLGEEKLQ"
     gene            1181..>3248
                     /locus_tag="K8M77_002373"
     CDS             1181..>3248
                     /locus_tag="K8M77_002373"
                     /inference="COORDINATES: protein motif:HMM:NF024685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBI7086825.1"
                     /translation="MEAFSNESKRIINKAARNNKLVVFVGAGVSMNSGYPSWQELIKD
                     FADGLGYDMKESSFDELLRIPQYYYNARKEKEYYDVIYNKFNIKSEPNKIHDLILELN
                     PAHIITTNYDDLIERAANSKGMFYDVVSKDSDLAYSVNNKMIIKMHGDLKNKNIVLKE
                     DDYMSYFNNFKLIQNYIKSLISTHVVLFVGYSVSDVNLKYIFQWVRDVLKNNFQQAYL
                     LECSKSFNQLEFEYYKNRGINIIYSKCPNFNYENKENDEEALNENLTIGQATEKMLKD
                     IVYEGENNGLTLEALYNVFKPLKYLEVIRIQDIIRPLKNLTSEELLYDFRNNIIELSN
                     TNLIEFFYDFNNEDLNYKKEFILEVLKKAKVKQIKVRKTKKQIENEILNKINDKRKSN
                     NNEENAEDVILNINLSKDEHKEYTYLFNYKVLEEKVNKNLFKGIKGREQEALLDAYYL
                     YKVGKFIDSYKILKTISEHCIEKNLYYFYFISEFNRYYLGKIISSRPWVYSEDGKLVK
                     SEYEKMDLEKVYKKLPKGFDNNIEVYKDILNFKFAYSQIKEVVSVWKNIDNERNTIYF
                     GADLNQFPIYKLINKMKMFNNFIVGNKLCVDTYNEIKVSFYNFIDCVLFSYTLEVKED
                     YNDFIWIKGNIPKILEIDYFIIYTMIEYLSFDEIKLLFDKYRIENLKVSKEALEDLKK
                     ISFNLFY"
BASE COUNT         1298 a          242 c          610 g         1098 t
ORIGIN      
        1 cggtggtgcc agttttaact ccgcaaatag cagtagtggt gggagaaatg ggtctagtgg
       61 tgtagatttt acatttatta tgaaacataa acttcttttt ggatcagtat cttttttctt
      121 gctgtctgga ggggtagttt taatgtttgt tgagatggat aggtatagta aagtggttaa
      181 gcgtaaaagt gataagctaa aggagttagc tttaaaggat ccttgctgga atattaatta
      241 tataaaatct gttgtggaag atacctttta tagtattcaa tatgcttgga atgatgatga
      301 atatgaaagg gtggagagtt tacttacaac ttcattatat aataagcatc ttaaggtttt
      361 agaggagctt aagagggaaa ataagaaaaa tgttttaaag gatattcagt ttaaggggtg
      421 tagaataata agtctctttg agggggatga aaagcaatgg ggatttattt gggttgaggt
      481 taatttctcc atgatagatt acatagttaa tacaaagggg aaaagaagga taatagaggg
      541 aaatgataat tgtagcagaa gtgactttga gtattggaag tttgtgtata ttgagaatac
      601 atggctactt tcaaatatat tgcagaaaaa tgaagtgggc tcaggctata agaagttata
      661 tagagaggct gaaaatgcct atggctctat taatatgaag aaggtttaaa atgtaggagg
      721 gagagaaaga tgactattga tatgggatgg atgattttag gctttggcgc atttatgttt
      781 ttaattgctt cagtaggtca gctaagaaat gaagttaaac ttatgagatg gactttaaat
      841 aggattgctg aaagggtagg agtaactgag atagtaacaa aggaagaaaa agaggaacta
      901 aagagattgc tttcagaagg aaaaaacgtc caagctgtaa gaaggtgcag agagattaca
      961 gaccttggct taaaggaagc taaggaatat gttgatggac ttggtgagga gaaattacaa
     1021 tagggtgtga gctgagtata aaactttatt tgttaagata aagacaatag tatattgtaa
     1081 tttgaatatt ggaaaaattc ataaggaata ttacctttga tttagttaga tagaagttta
     1141 tattttagaa attaaaattt ttttgtcgtg gaggagaaaa atggaagctt tttcaaatga
     1201 aagtaaaaga ataataaata aagctgcaag aaataataaa ttagttgttt ttgtaggggc
     1261 tggagtttct atgaattcag gatatccatc ttggcaagaa cttataaaag attttgctga
     1321 tggattaggt tatgatatga aagaatcatc ttttgatgaa cttttaagaa taccacaata
     1381 ttattataat gcaagaaaag aaaaagaata ttatgatgtt atttataata aatttaacat
     1441 taaatctgaa cctaataaga tacatgattt aatactagag ttaaatcctg ctcatataat
     1501 tacgactaat tacgatgatt taatagaaag agcagctaat agtaagggga tgttttatga
     1561 tgtagtatct aaagatagtg atttagctta tagtgttaac aataaaatga taataaaaat
     1621 gcatggcgac ttaaagaata aaaatattgt tttaaaagaa gatgactata tgtcttattt
     1681 taataacttt aaattgatac agaattatat aaaatcatta atatctactc atgtagtttt
     1741 atttgttggg tattctgtta gtgatgttaa tttgaaatat atttttcaat gggtaagaga
     1801 tgttttgaaa aataattttc agcaagcata cttattagag tgctcaaaat cattcaatca
     1861 attagagttt gagtattata aaaatagagg aattaatata atttatagca aatgtccaaa
     1921 ttttaattac gaaaataaag aaaatgacga agaagcactt aatgaaaatt tgactatagg
     1981 acaagctacg gaaaagatgc ttaaagatat tgtttatgaa ggtgaaaata atggtcttac
     2041 attagaagca ttatataatg tatttaagcc tttaaaatat ttggaagtta ttagaattca
     2101 agatataatt agacctttga agaatttaac aagtgaagaa ttattgtatg attttagaaa
     2161 taatattatt gaattaagca atactaattt aatagagttt ttttatgatt ttaataatga
     2221 agatttaaat tataaaaaag aatttatatt agaagtatta aaaaaagcta aagttaaaca
     2281 aattaaagtt agaaaaacca agaagcaaat tgaaaatgaa attttaaata aaattaatga
     2341 caaaagaaaa agtaacaata atgaagagaa cgctgaagat gtcatattaa acattaattt
     2401 aagtaaagat gagcacaaag aatatactta tttgtttaac tacaaggtct tagaagaaaa
     2461 agttaataag aatttgttta aaggaattaa aggaagagaa caggaagcgt tattagatgc
     2521 ttattattta tataaagttg ggaaatttat tgattcatat aaaattctta aaacaatatc
     2581 agagcattgt attgaaaaaa atctatatta tttttatttt atttctgaat ttaataggta
     2641 ttatttaggg aaaattatat cttctagacc atgggtatat tcagaagatg gtaaattagt
     2701 taaaagcgaa tatgaaaaaa tggatttaga aaaagtatat aaaaaattac cgaaaggatt
     2761 tgacaataat atagaagttt acaaagatat tttaaacttt aaatttgctt attcacaaat
     2821 aaaggaagtt gtatctgtat ggaaaaatat tgacaatgag agaaatacca tttattttgg
     2881 tgctgattta aatcaatttc caatatataa actaattaat aaaatgaaga tgtttaataa
     2941 ttttattgta ggaaataaac tttgtgtaga tacttataat gaaataaaag taagttttta
     3001 taattttata gattgtgtac tatttagcta tactttagag gttaaagaag actataatga
     3061 ttttatatgg attaaaggta atattcctaa gatattagaa attgattatt ttattatata
     3121 tacaatgatt gaatatttaa gttttgatga gattaagctt ttgtttgata aatatagaat
     3181 agaaaactta aaagttagta aagaagcttt agaagattta aaaaaaataa gttttaatct
     3241 tttctatg
//
LOCUS       DAEVGI010000203         3228 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEVGI010000203 DAEVGI010000000
VERSION     DAEVGI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.205"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            574..1167
                     /locus_tag="K8M77_002374"
     CDS             574..1167
                     /locus_tag="K8M77_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBI7086826.1"
                     /translation="MNKEMVDLLLIEGKKGNKEAMEKLFEIHKGLIYKYSTKYFIKGY
                     DREDLEQIGWIEFMNGVKKYDGSFGIDFVAFITILLRNKYGCMLKRKEVDMKFSSLDT
                     PIGEDITMKDTLEDDFSMEKDFEHREEMEALLNAISNLDDEEREFILYINEERGNMQK
                     YYEEHKNEMTFNAVRYKKKKIMDKIQKALKKKGIKVK"
     gene            complement(1391..1768)
                     /locus_tag="K8M77_002375"
     CDS             complement(1391..1768)
                     /locus_tag="K8M77_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086827.1"
                     /translation="MHKVTDIFISFILFIWGTLNWIYATRITIDINAFSSLKEVIIYI
                     IVTILYLIIQVLYINKSKIHILNILLLSLPTIIWFIILKNSLSIDGFFKYDGIAYTFG
                     FFVMIVVLFYNFYKIVLKYNVHS"
     gene            complement(1811..2062)
                     /locus_tag="K8M77_002376"
                     /pseudo
     CDS             complement(1811..2062)
                     /locus_tag="K8M77_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            2477..3133
                     /locus_tag="K8M77_002377"
                     /pseudo
     CDS             2477..3133
                     /locus_tag="K8M77_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1290 a          330 c          555 g         1053 t
ORIGIN      
        1 gggaaaaata aaaaatagta taaaaaaaag acatttatta aataccctat atatagtgag
       61 tgaaaaatag caaaagtctg gtaaagtctg tctgaagtta gttatatcaa gggtttagag
      121 tgtgacggac tttttaaaag tccgtcacag tagatgtttt tattgaggtt tttaagtatt
      181 tattatgaaa ttaggtgaaa aaatagtgct ttttaaataa aaatggacat gctctgggat
      241 aagaataata tgctttagaa gtttttaata aaggtggaca aaccttggta ttgttatgat
      301 attagattta ataaatttca ggcttttaaa ataaaaatgg acaagctttg aaaggggtta
      361 aaaatgttga ctagttggta aagggtggat aaagtaagat gttttaagga ttttagcttt
      421 tatatatatg gaataaaagt tggcatttat atagaaaaat attactttat ccataaaaat
      481 atagatttta aggcttttta atagaaattt tatgtgaaaa ataattaatt tgaatatagg
      541 aaataaaata taaggtttag gggtataggg tttatgaata aggaaatggt tgatctttta
      601 ttaatagagg ggaaaaaggg aaataaggaa gccatggaaa agctttttga gattcacaaa
      661 ggattgattt ataagtattc aactaagtat ttcataaagg gatatgatag ggaggactta
      721 gagcagatag ggtggattga gtttatgaat ggggtgaaga agtatgatgg aagctttgga
      781 atagattttg tagcttttat aaccattctt ttgagaaata aatatggctg tatgctaaaa
      841 agaaaggagg ttgacatgaa attttcaagc ctagacacac caattgggga ggatataaca
      901 atgaaggata ccttagagga tgatttttcc atggaaaagg actttgagca tagagaggaa
      961 atggaggcac tattaaatgc tattagtaac ttggatgatg aggaaaggga atttattctc
     1021 tatatcaatg aggagagagg aaatatgcaa aagtattatg aggaacataa gaatgaaatg
     1081 acctttaatg ctgtcagata taaaaagaaa aagattatgg acaagattca aaaagctctt
     1141 aagaagaagg gaataaaggt aaagtaaggg gggagctagt ggattagaaa tattgtggaa
     1201 aaacttaaaa aaatgcttaa ttatagatta ataaaggtta agtagagatt tatttctagt
     1261 gtgggaaata ttatggacaa gctaccaatt atattaaaat aaggttctat tttaaatcaa
     1321 gattgataat agatagtata aaagtggaca aatagtaatc ttgtccactt ttattttaaa
     1381 tcgcaatata ttaagagtgt acattatatt taagaactat tttataaaaa ttataaaata
     1441 atacaacaat cataacaaag aatccaaatg tataagctat tccatcatat ttaaaaaatc
     1501 catcaatgct caatgaattt tttaaaataa taaaccaaat tattgttggt aagcttaata
     1561 ataaaatatt aagaatatga atcttacttt tattaatata aagaacttga ataattagat
     1621 ataaaattgt tactataata taaattatta cttcttttaa tgaagagaat gcgtttatat
     1681 caatagtaat tcttgttgca tatatccaat ttaaagtacc ccaaataaat aatataaaac
     1741 taataaatat atctgtaact ttatgcatat atccctcctt attaattaat aatttaatac
     1801 gattgttcaa aactttatct ttgctaatac ctcgcttttt gaattgctta cctctcttac
     1861 gagaaggtct tggcatattc acaggtttag ttcccttata ataataagca aagaaaactt
     1921 catctgcttc aaccacacca tcaacagaac ctacaccaag aaacttactg atacaattaa
     1981 gtatcttatg tggccagtaa aaagaagttg caatattaat ttctacttct tctgctgatt
     2041 ttcttataga aaaaccattt aattctaatt gagtcaatag ttcttgttgt actttccctg
     2101 ataaggcttt tgcttatgcc aataatgatg ttagactgct ttgctcatag tgatacctcc
     2161 taaaaattga ttatatacat ataataacct atttttgtaa aattatcata tatcaaccac
     2221 tatttaaaac agagcctaaa ataaaatttt taactgagaa aaagattaag ataagatact
     2281 taaattgtgt taagtgtaat ataagaataa agtttaaaaa ggtattttat gctataatta
     2341 ttaaaatatc ttaaattaaa aaatacaata tatttgaaag gaatagagaa tgaaaaaaag
     2401 aaaaagactt tttctaagta tcattttttt cggtacaatt ttaaatttac ttcttatatt
     2461 tttaggactt aaagtattgg taaagaggtt atagataaaa atcaatgaag agccaaaagt
     2521 ggtggaggca caatttcctt acaatacaga atttgataat tacaatatag aaataaagaa
     2581 gcctaagtta gtaaatcact acattctctt tgattacact atttccacaa aggacaattc
     2641 tcctatagaa gaggatgatt ttcacattac aggtagtcca agctttccag acacagcata
     2701 ttttgtatca gcccatgata ttttaagaaa agtagagaat aataagatga gaattgttaa
     2761 tgttggaata attgtagcta gaaaagacta tgataaatat gttaataatt ttgatttttt
     2821 agtaagtgct agagaaaagg gaagcagtaa aaatctcttt gaggaaaaaa gggtagacac
     2881 aaccataaaa attgatggag tagtttatcc agataaggaa aactacaaag agattaaaaa
     2941 gacttttaaa ctagatggta ttgattttga agttttaagc ataggagatt ttgactttgg
     3001 aagctgtata cctatatttg taggaaatat aagtgaggaa aaaggcaaag aaatagaaaa
     3061 taaatatgct cttagaattg aaggggaaaa tataaatgca atgtataacc tggaaaaatg
     3121 ggttggattt tatgcctttg gcaagaacac ctgtgatttc aatcatggga tgaattgcta
     3181 ctacatatgc taaaagtata aaccgtctaa cacttgcaaa taagttat
//
LOCUS       DAEVGI010000204         3198 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEVGI010000204 DAEVGI010000000
VERSION     DAEVGI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.206"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            140..3031
                     /locus_tag="K8M77_002378"
     CDS             140..3031
                     /locus_tag="K8M77_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7086828.1"
                     /translation="MSVLQDIKEKVIKYKIEEVGSKGIIEDKYLIKNRLIVNSQRGNL
                     LGELKESLRECKSFYFSVAFINFSGLQLLLDSFRDLEERGVKGKIITSTYLNFTEPKA
                     LENVKSFENIDLKIFIADSEKGFHTKAYIFENEDNFKIIIGSSNITQRALKSNVEWNV
                     MIISKKDTDFVKEVLEEYEGIWSSTGIVDDEFLEKYNEFIKSIKEKQREIAIDFSEYE
                     VIKPNFMQKEAMENLERLRRAGEDKALVIAATGTGKTYMSAFDVMAFNPKRLLFLVHR
                     EDILRSAEKTYKSLCKNKNITMGFYTGNSKEKEADYLFSTVQTMCRNLEDFNRDDFDY
                     IVIDEAHHSTSPTYERILNHFTSKFLLGITATPERCDTGDIFQVFDNNIALEIRLNEA
                     LEHELIVPFHYFGITDIDAVDYSNVDLDDISEISKRLMINSRVDFIIDKINFYGFDGS
                     KQKTVGFCINKEHAYYMACKFNEAGIPSIALTGDHSIDKRTEEIKNLENENHELKVIF
                     TVDIFNEGVDIPSINQVLMLRPTNSPIIFIQQLGRGLRKHRSKEFLTVLDFIGNHNKA
                     FLIALALKGGRYYDKDSLKVSVATDFSDIPGCSNIQMDRISKERILNQLNTENFNSLK
                     YLKEEYNEFKSLLGGKIPYQLMDYVKFDGAPDPLKFINYGKTYQGFLNKVEKTYEALE
                     LLDEDFYKVLKELSDKLPLKRPHEFAIIKELINKSELNLDEASKGIRKYIQSVDEETV
                     KHAFRTLNQDYYDSREKSNFIKLFDLIGERLVKLLSFEKILSDKRYKEIIVDLLNYGL
                     ITYEKRFGSENFGVPFFKLYEQYQMVDAALLSNYEKIHSSFRGSGLMANGKEYFLFVD
                     LNKDKDIKESINYKDEFLSRSLFQWETPNSTAQSSERGKNIIFNKERGINLHLFVRKY
                     KEIDRKVQPYIYIGKGDVVKYEGEKPIKVHIELHNRIPENLYVELMKKV"
BASE COUNT         1213 a          333 c          632 g         1020 t
ORIGIN      
        1 gaaataaaat tgttttaatg ttaaaataaa acagtaaata caaatatata aagaggggtt
       61 ttacgcttta tagttattat taaagtaagg aaactactgt ttttatatat ttgtatattg
      121 taaaattagg agcattagaa tgagtgtatt acaggatata aaggaaaaag ttataaagta
      181 taaaattgaa gaggtaggat ctaaaggcat cattgaagat aaatatctta ttaaaaacag
      241 gcttattgtt aattcccaaa gaggaaatct tttaggggaa cttaaggaga gtctaaggga
      301 gtgtaagagt ttttatttta gtgttgcctt tattaatttt agtgggcttc aacttctttt
      361 ggattctttt agggatttgg aggaaagagg agttaagggg aagataataa cttctacata
      421 tttaaacttt actgagccaa aggctttgga gaatgtaaag agttttgaga atatagattt
      481 gaagattttt attgctgaca gtgaaaaggg gtttcacact aaggcttata tatttgagaa
      541 tgaggataat tttaagatta taattggttc atctaacata actcaaaggg cattaaagag
      601 caatgttgag tggaatgtta tgattatttc taagaaggat actgactttg ttaaggaagt
      661 tttagaggaa tatgagggaa tatggagctc aacaggaatt gttgatgatg aatttttaga
      721 gaaatacaat gagtttatta aaagtattaa ggaaaagcaa agggaaatag ctattgattt
      781 tagtgagtat gaagttataa aacctaattt catgcaaaaa gaggctatgg aaaaccttga
      841 aagattgaga agggctggag aggataaggc tttagttatt gcagcaactg gaacaggaaa
      901 gacttatatg tcagcctttg atgttatggc ttttaatcct aaaaggttat tattcttagt
      961 tcatagggaa gatattttaa gaagtgcaga gaaaacttat aaaagcttat gtaagaataa
     1021 aaatatcacc atgggatttt acacaggaaa ctcaaaggaa aaggaagctg attatttatt
     1081 ctctactgtt caaactatgt gtagaaattt agaggatttt aatagggatg attttgacta
     1141 catagttata gatgaggcac atcatagtac aagccctact tatgagagaa tattaaatca
     1201 ttttacctct aagtttttac ttggaataac tgcaacccct gagagatgtg atactggaga
     1261 tatttttcag gtttttgaca ataatatagc cttagagatt agattaaatg aggctttaga
     1321 gcatgaactt attgtaccat ttcattattt tggaataact gatatagatg cagttgatta
     1381 tagcaatgtt gatttagatg atatttctga gatttctaag agattaatga ttaactcaag
     1441 ggttgacttt attatagaca agataaattt ctatggtttt gatggaagta agcaaaaaac
     1501 agtaggattt tgcataaata aggaacatgc ctattacatg gcttgtaaat ttaatgaggc
     1561 tggtattcct agtattgctt taactgggga tcattctata gacaagagaa cagaggaaat
     1621 aaaaaatctt gagaatgaaa accacgaatt aaaggttata tttacagtgg atatatttaa
     1681 tgaaggggtt gatattcctt caattaatca ggtcttaatg cttagaccaa ctaattcacc
     1741 aattatattt atacaacagc ttggaagagg attaagaaag cataggagta aagaattctt
     1801 aactgtttta gattttatag gaaatcataa caaagccttt ttaatagcct tagctttaaa
     1861 aggtggaaga tactatgata aggatagttt aaaggtttca gttgctacag atttttctga
     1921 tattccagga tgctctaata ttcagatgga tagaatttct aaggagagaa tattaaatca
     1981 gcttaacaca gagaatttta atagcttaaa gtatttaaag gaagaatata atgagtttaa
     2041 atctctttta ggtggaaaga ttccttatca gttaatggac tatgttaagt ttgatggagc
     2101 accagatccc cttaagttta ttaattatgg aaagacatat cagggatttc ttaataaggt
     2161 tgaaaagact tatgaggctt tagagctttt agatgaggat ttttataagg tgttaaagga
     2221 attatcagat aaattacctc taaagagacc tcatgaattt gccataataa aggaattaat
     2281 aaataaaagt gagttaaatt tagatgaagc ttcaaaggga ataagaaagt atattcaatc
     2341 tgtagatgaa gaaacagtta aacatgcctt tagaacacta aaccaagatt attatgattc
     2401 aagggaaaag agtaatttta ttaaattatt tgatttaatt ggagagagat tagtaaagct
     2461 actaagcttt gaaaagatac tatcagataa aaggtataaa gaaatcatag tggacttgtt
     2521 aaactatgga cttataacct atgagaagag atttggtagt gaaaactttg gagtgccatt
     2581 ttttaagcta tatgaacaat atcaaatggt ggatgcagca cttctatcta attatgaaaa
     2641 gatacatagc tcctttagag gaagtggact aatggcaaat ggaaaggaat atttcctttt
     2701 tgtggactta aataaggata aagatataaa ggaaagcatt aactataaag atgaattcct
     2761 aagtagaagc ttgttccaat gggaaactcc aaacagtaca gcacaaagct ctgaaagagg
     2821 aaaaaacata atattcaata aagaaagagg aattaacctg cacctttttg ttagaaaata
     2881 taaggagata gacagaaaag tacagcctta tatctatata ggaaaaggtg atgtagtaaa
     2941 gtatgaagga gagaaaccaa taaaagttca tatagaacta cataatagga ttccagaaaa
     3001 tttgtatgtg gagcttatga agaaggttta aaagattaga taatcataaa gtttacaagt
     3061 gaggtttgga tttgtgtcaa tatcttggac acaaaaattc gaacttactt aagctgcatt
     3121 caaagcctta aattctacct cattaggagt attataatca agagctgaat gcagtctttt
     3181 actattgtaa aaactttc
//
LOCUS       DAEVGI010000205         3185 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEVGI010000205 DAEVGI010000000
VERSION     DAEVGI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.207"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            370..1059
                     /locus_tag="K8M77_002379"
                     /pseudo
     CDS             370..1059
                     /locus_tag="K8M77_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458960.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            1154..1408
                     /locus_tag="K8M77_002380"
     CDS             1154..1408
                     /locus_tag="K8M77_002380"
                     /inference="COORDINATES: protein motif:HMM:NF021575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HBI7086829.1"
                     /translation="MRYTLVWFIAGISMLLLSLNSGILNYFSKILNIIYPPSLLFLIG
                     ILFCFILILSLIVTITSLQIKLTRLTQEIALLKNEKENKE"
     gene            1405..1752
                     /locus_tag="K8M77_002381"
     CDS             1405..1752
                     /locus_tag="K8M77_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBI7086830.1"
                     /translation="MIYPLLILNILLLVCGQTLWKIGLKNKTLAFTFDSIINLIFNPY
                     ILCGVLLYGVATLIWLYILSKSDLSLVYPLQSLCYVLALFVSIFILNESVPTNRWIGV
                     GFIILGSFFIGIK"
     gene            1767..3047
                     /locus_tag="K8M77_002382"
     CDS             1767..3047
                     /locus_tag="K8M77_002382"
                     /inference="COORDINATES: protein motif:HMM:NF024629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HBI7086831.1"
                     /translation="MFSLKEESKKTKLILYTIIFIFFIIGTYTVIKYGNNYLLGNFNE
                     WNNDDVKYVRSAWTLLDNGKFTYHNVNDETVFIMPGLPYTMAFFMSIFGKFGGITAFR
                     IFQVVLMSLSFSLIFFIGRFLFNSKVGIISSILSILYLPNLFTPNLLLTEVIFYFLIL
                     LLVYTTLVAIKSKKIKHYIIGGIVWGIAALFRPTVGAYPIVVLILWIYNNYKFKDILK
                     FTIIVSSVFCLVMSPWWIRNYKEFHRFIPFTLSSGNPMIQGSYINYDTTIDFHPWEIT
                     DDIIKQEEYYKDNLVYRFKNYILKKPLTYAKWYLIDKTVILWISPFYWKPVYNIHIVF
                     VGIYHLLLLTLGFIGMYSIHKKSKNNKVDKTARILFGLFIYYSVVHIPYITFNRYSYP
                     MIWIFVISSAFVINNIITQKQERSKQILNTNNLE"
BASE COUNT         1193 a          344 c          418 g         1230 t
ORIGIN      
        1 taaataaact tgaatttaaa cttaaaaatt taagttttga ctgaatctca catataaaac
       61 taatctatgt atttaagttt tggcagaaaa aaatatctag cttatagcta gagattctta
      121 atattataaa atattttttc taatatattc ttataagcaa ttgtacttaa tccacctgcc
      181 ttacacctat aataattaaa tcaatacata attctatata ttaacctttt tttatcaatt
      241 ataaaacttt atttaaaata tattttaaaa ttattataat tatatgtaat ttaatatata
      301 tttttgcaaa atattgtata ttatgttaat atatcttaat atagaagtat agttgaaagg
      361 agctattaaa tgaaaatttt agtgattatc cctgcatata acgaagaaaa atctatttct
      421 aaagttatta tggatatcta taatcaaagg atagaagatt tagatatctt ggttataaat
      481 gatgcttcta gtgataatac caagttttaa gcacaaaaat taggagttaa tgttgtagac
      541 ttacctataa atttaggaat aggagtagca gttcaaactg gttatatata tgcttacaat
      601 aataattatg atgtagccat tcaatttaat ggagatggtc aacatatggc aaaatatctt
      661 aatgatatga taaaagccat aattaatgaa aattatgata tggtagtagg atctagattt
      721 ataaataata gcaattataa gcctagtatc tatagaaaat taggaataaa cttcttttca
      781 tcacttgttt caattttagg tagaaaaaaa gtttttgata ctacatcagg atatagagct
      841 gtaaataact ccgtaattaa ggagtttgta gaatattatc ctaaagatta tccagaagta
      901 gaagcactat catatattat tagaaaaggg tttaaagtta aagaaattcc tgttgaaatg
      961 aactatagag aatctggaaa atcttctata accccactaa aatctattta ctatatgcta
     1021 aaagtaactt ttgcaactat aatttcacct gataaataag aaaggaacat ttttatgtat
     1081 tggtattgcg ttataatagc attaatattt ttaattttga caatagtatt tataaaaaaa
     1141 acgattatta gacataagat acactttagt ttggtttatt gctggaataa gcatgctttt
     1201 attatcatta aatagtggta ttttaaatta tttttcaaaa atactaaata taatctatcc
     1261 accatcattg ttatttttaa ttggaatttt attttgtttt atattaattt taagtttaat
     1321 agttacaata acatccttac agattaaact tactagactt acacaagaaa ttgctttact
     1381 aaaaaatgaa aaggagaaca aagaatgata tatcctttac tcatattaaa tattttacta
     1441 ttagtatgtg gacaaacctt atggaaaata gggttaaaaa ataaaacttt agcttttaca
     1501 tttgatagca taattaattt aatatttaac ccatatattt tgtgtggggt actgctatat
     1561 ggtgtagcta cacttatttg gttatatata ctttcgaaat cagatttatc tttagtatat
     1621 ccattacaaa gtttatgcta tgttttagca ctatttgttt ctatctttat cttgaatgaa
     1681 agtgtcccta ccaatagatg gataggtgtt ggttttataa tattaggttc attttttatt
     1741 ggtattaagt aagggggaga ttcacattgt tttctttaaa agaagaaagt aagaaaacta
     1801 aattgatatt atatactatt atattcatat tttttattat aggaacttat actgtaatta
     1861 aatatggaaa taattatttg ctaggtaact ttaatgaatg gaataatgac gatgtaaaat
     1921 atgttagaag tgcatggacc ttattagata atggtaaatt tacatatcat aacgtaaatg
     1981 atgaaacagt atttataatg cctggattgc cttatactat ggcattcttc atgagtatat
     2041 ttggtaaatt tggaggtata actgcattta gaattttcca agtagtcctt atgagtttaa
     2101 gtttttccct aatatttttt attggaagat ttctttttaa ttctaaagta ggtattataa
     2161 gttcaatatt aagcattcta tatctaccta atcttttcac gccaaattta cttctaacag
     2221 aagttatatt ttatttttta atattactat tggtatatac cacattagta gcaataaagt
     2281 caaagaaaat aaaacattat attataggcg gtatagtatg gggaatagca gcactattta
     2341 gacctacagt tggagcatat cctattgtag tgctaatttt atggatatat aataactata
     2401 aatttaaaga tatacttaag tttactataa tagtgtcatc agtattttgc ttagtaatga
     2461 gcccttggtg gataagaaat tataaagaat tccatagatt tattcctttt accttgtcat
     2521 ctggaaatcc tatgatacaa ggttcatata taaattatga tactactata gattttcatc
     2581 cttgggaaat aactgatgat ataataaaac aagaggaata ttataaggat aatttagtgt
     2641 atcgatttaa aaattatata ttaaaaaaac cactaactta tgcaaaatgg tatttaatag
     2701 ataaaacagt tattctttgg ataagtccat tctactggaa accagtttat aatatccata
     2761 tagtttttgt tggaatttat cacttactat tattaactct tggatttata ggtatgtatt
     2821 caatacataa aaaatctaaa aataacaaag ttgataagac agcaagaata ttatttgggt
     2881 tatttatata ctacagtgta gttcatatac cttatattac atttaatagg tattcttatc
     2941 caatgatatg gatctttgtt atatcttcag catttgtaat aaataatata ataacccaaa
     3001 agcaagaaag atcaaaacag atattaaata ctaataatct agagtagctt tataattatc
     3061 ttaataagtc taaattatat ttaaggggga tattctatgg ctttattcag tattttttct
     3121 atattttatt tgatctttat cttaactatt ggatttttag gaatatatgc acttatttta
     3181 cttat
//
LOCUS       DAEVGI010000206         3164 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEVGI010000206 DAEVGI010000000
VERSION     DAEVGI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.208"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            165..1094
                     /locus_tag="K8M77_002383"
                     /pseudo
     CDS             165..1094
                     /locus_tag="K8M77_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099813.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
     gene            complement(1284..2027)
                     /locus_tag="K8M77_002384"
     CDS             complement(1284..2027)
                     /locus_tag="K8M77_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086832.1"
                     /translation="MINQRVFFNILDEDQKPITKCVVKIENQKEKYEGETDLAGIVEF
                     LVPLGIYVLTIEHTAFKKKIYRMILRDGFSFTRIILQSNKPNYKREEKNYILEDADKD
                     FLNNGILKDNIEKIYDINKNIDEDLNSEENSRDNFSEKEEIFKDYFHNLDLFQNPSIW
                     GETGIFKKVNENSFNEIIKGAKTMLEEILNLASSFEAEDFKKDENNMPFTFDEIKEES
                     KNIANNIIDKDYGSKTYDVCDYEEEFFDK"
     gene            complement(2246..2605)
                     /locus_tag="K8M77_002385"
     CDS             complement(2246..2605)
                     /locus_tag="K8M77_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086833.1"
                     /translation="MRFIFFIALIFIIVEIGISSYLVYDLHCKNDEEIKNLEKQDVKT
                     IVTLRKVVVLDNFISLLLYSVLITNKDILKFGRGEILTVAILLSIGVMMIYLYMFDNF
                     IEKFKKFNNTEKENSIA"
     gene            2777..3112
                     /locus_tag="K8M77_002386"
     CDS             2777..3112
                     /locus_tag="K8M77_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBI7086834.1"
                     /translation="MENIKSIFYFLLAGVFEIGGGYLIWLWLRQGKSLIYGIIGALVL
                     ILYGIIPTLQPENSNFGRVYATYGGIFIVLSILCGWKVDNIIPDKFDLIGGFIALIGV
                     LIIMYAPRG"
BASE COUNT         1173 a          436 c          295 g         1260 t
ORIGIN      
        1 acactccttc acttaactta ttatgattat tatacacaaa gcttatttta tttggctata
       61 ttattgaaaa tttatctctt aaatcatatt ttaatacata taacaaattg tttaatgaaa
      121 tactaaagtt attatttatt acaaaatata ttaaggtgat tttactgtat ttaaacattt
      181 taagaaaaac gcacttatta ttttttttat tattaagtat ttttactagc ttttttatta
      241 gtaatacttt agttgtttca gcaaaagata atgatgtaca tttagtttta gatactaaaa
      301 atattaataa tcttccaaat aattttagaa caacatctga tttagaaaga ttaaaaaacc
      361 tatctaatat taatatgaaa ggtttagata ctctcaatat ttcaggcagt caacaatttt
      421 ctcctaataa cctttaactt ttagttactt caattaaaac cactttacct ataacaattg
      481 tagatttaag acaaaaatct catggattta ttaatgaata tcctgttagt tggaaaggcg
      541 aaaaaaatga tgccaactta tgtttgacca gaacagaagt tattgatact gaaagaaagc
      601 ttttaaacag tattacctta gcaactccaa tacaattttt taatgatcct aagctaactg
      661 ttatccctga gaaagttctt tctgaaaatc aattaataaa agctaattct atggattatg
      721 ttagaattcc agtaactgat gggaaacttc ctacttacga aatggtagat ttttttgttc
      781 aatatgtaaa ttcgatacca aaagattcat ggctacattt ccattgtaag gaagggatag
      841 gaagaacaac aacttttatg attatgtatg atataatgaa aaactataat aatgctactc
      901 ttgatgaaat aataaataga caattagctc tctctagaat taaggaaaaa tctatacttt
      961 ccttcccttc aaaagagcgt ttagacttct ttactaaatt ttatcaatat gtaaaagaac
     1021 aaaataatga ttttaagact agttggagtc aatggcttaa taaaaataat tttcctctag
     1081 ctactatacg ttaaatatat aaatattatt taaagaaaaa ggatgtatta aaagttaaac
     1141 ataaaactta tagataataa gaaatacctt ataagtttta tttatgaatt attttaatac
     1201 atcctttttt aattatttct gcatattcaa ttaaaacaat aaaagttaag taaaattctc
     1261 attcactact taaattcact tttctattta tcaaaaaact cttcttcata gtcacaaaca
     1321 tcataggtct tactaccata atctttatca ataatattat ttgcaatatt tttactctct
     1381 tcctttattt catcaaaagt aaaaggcatg ttattttcat cttttttaaa atcttcagct
     1441 tcaaaggaac tagctaaatt taatatttct tcaagcattg tttttgctcc cttaattatc
     1501 tcattaaaac tattctcatt tactttctta aaaatacctg tttcccccca tatacttggg
     1561 ttttggaata aatctaaatt atgaaaataa tccttaaaaa tttcttcttt ttcactaaaa
     1621 ttatccctag aattctcttc tgaattcaaa tcttcatcta tatttttatt tatatcatat
     1681 attttttcta tattatcctt taaaattcca ttatttaaaa aatctttatc agcatcttct
     1741 aaaatataat ttttttcttc tctcttataa tttggtttat tgctttgaag aattattcta
     1801 gtgaaactaa atccatccct taaaatcatt ctatagattt tcttcttaaa agcagtatgt
     1861 tctatggtta atacataaat ccctaatgga actaaaaatt ccactattcc agctaaatct
     1921 gtctcacctt catatttttc tttttgattt tcaattttta ctacacattt tgttataggt
     1981 ttttgatctt catctaaaat attaaaaaaa actctttgat taatcataat atctattaag
     2041 attttaccct cttaatattt tcacactccg aagaatatta tacattatat aattattctt
     2101 aaattttatt tttgttaata aaattacaaa ataaaaagca cgaaataaaa ccgtactttc
     2161 ttattaatca tattttatag aaattcttca ctatttactc taatttttca taaaaaattt
     2221 aaacattata gatttaaaat agcttttaag ctatactgtt ttctttttca gtattattaa
     2281 acttcttaaa cttttcaata aaattatcaa acatataaag ataaatcatc attacaccaa
     2341 tagataataa tattgctaca gttaatattt ctcctcttcc aaactttaat atatctttat
     2401 tagttattaa aacactatat aataacaaag atataaaatt atctaataca actacttttc
     2461 tcaatgtaac tattgtttta acgtcttgtt tttccaaatt tttaatttct tcatcatttt
     2521 tacaatgaag atcatataca agataagaac ttattcctat ttcaactatt ataaaaatta
     2581 aagctataaa aaatataaat ctcatgatcg ccccccctaa attctttaat atcttgtaat
     2641 cgtttcacaa actattatat catcatattt ttatatttcc aaccaaatat attcaaaatt
     2701 aaatgaattg atgaatcttt atatgaatga tataatttgc atacttaaat taattactag
     2761 aagaggagat tttaatttgg aaaatataaa atcaatattt tattttttat tagctggagt
     2821 ttttgaaata ggtggtggtt accttatatg gctttggctt cgccaaggaa aaagtttaat
     2881 atatggaatt ataggtgctt tagttctaat attatatgga attattccaa cacttcaacc
     2941 tgaaaactct aattttggaa gagtttatgc tacctacggt gggatattta tagttttatc
     3001 aatcttatgt ggatggaaag ttgataatat tattcctgat aaatttgatt taattggtgg
     3061 atttattgca ttaattggag ttttaattat aatgtacgct ccaagaggtt aattataaat
     3121 aaaaataaaa ataaatttaa ttctcaaaat aaagcaaaag ataa
//
LOCUS       DAEVGI010000207         3158 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEVGI010000207 DAEVGI010000000
VERSION     DAEVGI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.209"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(813..1313)
                     /locus_tag="K8M77_002387"
                     /pseudo
     CDS             complement(813..1313)
                     /locus_tag="K8M77_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590527.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1586..1816)
                     /locus_tag="K8M77_002388"
     CDS             complement(1586..1816)
                     /locus_tag="K8M77_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4318 domain-containing protein"
                     /protein_id="HBI7086835.1"
                     /translation="MFKKGFLIDLDVEPKTDEDICLEVAKYCGKDGLAFEFIKRTSPV
                     IAMIDGTKYEIQKKYQTVRLLNCWVLSCSEID"
     gene            complement(2176..2406)
                     /locus_tag="K8M77_002389"
     CDS             complement(2176..2406)
                     /locus_tag="K8M77_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4318 domain-containing protein"
                     /protein_id="HBI7086836.1"
                     /translation="MARKCFFIDLDDMPKTDEDICLGFEKYCGENNLPFVFMKKTSPV
                     IAKIKDNNYEITKKYENGRFLNRWILSCKAVN"
BASE COUNT         1029 a          403 c          349 g         1377 t
ORIGIN      
        1 aattactact tttctatata aattataaga atcttcatat ttaaatataa attaagttat
       61 aaaaatagag agctaaattt atatagtatt gggaaataat taaaaaatag tgtttaattc
      121 gttacaataa agatatatag aacattttga taagtataat tttaagaagg tgattttatg
      181 tttcagttta ttattatagg agctgtagga atacttttct ctgttttggg atatttagta
      241 atggttaaga aaaaaacaag cttaatacac cattatcatc taaggggagt aaaggacatt
      301 aaaaattatt gcagctttat gtgaggatgt ttattccttt taggcgtagt atttataggc
      361 ttttctattt taggctttac tgaaatttta acttttgcac aaatgcagct tagtatagtt
      421 attctttgta ttttagatgt tgtagcactt ttagttatac aggaaaagtt tgcagggcat
      481 atattttagg gaaattactt taatatagga gtgattactt tctctaaagt tattgaaatt
      541 ctcacattgt tatatgagat aataaaccct aattatataa gttaggaaaa tcaaaaatga
      601 aaagtataat aaagcttttt tccttgctta agataaaaga accttatcat tttaggctaa
      661 atgataaggt tctttactaa atgaatatat taaaaaacaa ataaacctta tcatttgcaa
      721 gtaaatgata aggtttattc ctattttctg ttaatcttat tttatgatta tattaaaaat
      781 tctttgctga tatttaaagc ttccttatct ttgtagattt cttctccact gctctttaaa
      841 gcttccttgt gtttatatgt ttcttcctca ctgttattga aatattcctt tactttatag
      901 atttcatttt cactattgtt gaaaattcct ttatctttat aagttttctc ttcaactttc
      961 tttacttcca cttgattttt gtggacttta atctttaaaa tcccatagtt tattattttt
     1021 atgttatatt ctccatcttc tagatttgag taagtagatt ttagttttag cattattctc
     1081 actatatttt catagttaaa tattctgaaa ttattatttt tagtaattga gtaattttca
     1141 catatcttta aaagctgtga tattttctct ctttcatata ctggaaccct tacatttact
     1201 tccctatgat taaaagtttt tatgtatgaa gctatgacaa agtttaaatc attattatcc
     1261 tctataacta tttcttcaat aaagccattg tttgaggaaa tatgttaaat aaaaatagtc
     1321 tataccatgg ttattataaa aatcagtaat attatgagtt aagttataaa aaatagatag
     1381 acctaaaaat gtgatctacc tatttttctt ttatttttaa tttataatgt actaaaacta
     1441 taaggataaa ttttggcaaa tttttaatat aactttttat ttggattttt ttaatatgtc
     1501 tctttatttt agtatggtcc atgtaaaaac tctgtttata gataattttt ttaataagca
     1561 tttcatatct taagataatt taatattagt ctatttcact acagcttaaa acccaacagt
     1621 taagtaatct aacagtctga tatttctttt ggatttcata ttttgttcca tcaatcatag
     1681 caattacagg tgaagttctt ttaataaact caaaggccaa gccatctttt ccacagtatt
     1741 ttgcaacttc taagcaaata tcctcatctg ttttaggttc aacatctaag tcaattaaaa
     1801 agcctttttt aaacataaat tttaatcccc cttaataaag ttaattttta agtttattat
     1861 attggataaa ttgattttaa aattaactat tttgcaccat ttgaaagtag ctattttcta
     1921 tgctatatag ttattataag tagttcacta ctcttttcat taagcattta tgaattctgt
     1981 tatatatact atataattat tatccatatt tttcaatata ttgagaattt taaataaagt
     2041 taatgcttta atttattctt ttgccactta aaaaggtaga atcattttaa aacccactac
     2101 actttaaact tttatttaat aatttacaac ttaaaacagt agaatcattt tcaaaaatcc
     2161 accacgcttt atgccttaat ttactgcctt acaacttaaa atccatcgat taaggaacct
     2221 tccattttca tatttctttg tgatttcata attattatcc ttaattttag caattacagg
     2281 tgaggttttt ttcataaaaa caaagggcaa attattttcc ccacaatatt tttcaaaacc
     2341 taaacaaata tcttcatcag tttttggcat atcatctaag tcaataaaaa aacattttct
     2401 agccatagtc tctactcccc taaaaattta tttttctctt ttttcctaaa aagatttttc
     2461 ttataaaaaa atttacttca tttattcaaa tatatataaa tacccataga ttttattcct
     2521 tcatgtccta taattttctg taaaagtatt tttacttaaa tgtatttttt aactttaaaa
     2581 tatatttgct tgaagagatt tttacttgaa gatacttttt tgccttagat attttagggc
     2641 ttttatagtt ttttacactt tatatttttt cttagggtga ttttcctttg aaggcattat
     2701 catcaactta ataaaagttt ttacttagga ttaataggat tgcattttat aaggcaagat
     2761 ttgattatca ccttataaaa gtttttactt aggttagctt tttattaatt taggggtaac
     2821 ttagagataa ttttgattta tagattttta tttgctggat gttttttact ttatattaaa
     2881 aagatttgca ataaggtttt tactgcaaat cttttatgga tgtgaattta atttatattt
     2941 acttcttttg taacttaatt tatctttact tcttttatag atgtaagtct aatttattct
     3001 tacttcttca ttagtttcct tgtttcttag atatattttt acattgtcct tatttacttc
     3061 ttttggatta tagaagattt ttcccatgta gaactggtca tactgtgagt ttttatcata
     3121 acttgtgtcg atattactgt atctttttat tacttgct
//
LOCUS       DAEVGI010000208         3122 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEVGI010000208 DAEVGI010000000
VERSION     DAEVGI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.210"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            177..3098
                     /locus_tag="K8M77_002390"
     CDS             177..3098
                     /locus_tag="K8M77_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI7086837.1"
                     /translation="MNFKENNIYSGFKLLKIENLNEIGGLGLRFEHEKTKAKLIKILS
                     EDDNKCFAIGFRTPSENSTGVPHILEHSVLCGSRKFNTKEPFVELLKGSLNTFLNAMT
                     YPDKTIYPVASRNEKDFINLMDVYLDAVLYPNIYKHKEIFMQEGWHYYIENKEDELKY
                     NGVVYNEMKGAYSSPDSILYRKIPQTIYPDTCYALSSGGDPDEIPNLTYEEFVEFHKK
                     YYHPSNSYIFLYGNGDTEKELEFINEEYLKNFEYKEIDSEIKEQKSFESMKEESFTYG
                     IAESEDLNHKSYYSLNFVIGDATDGEKGLAFDVLAYLLTRSTAAPLKKALIDAGIGKA
                     VSGDFDNSTKQSAFTVLVKNAELNKEEEFKKVVMDTLKDLVENGIDKELIEASINRVE
                     FELREGDYGSYPNGLIYYLKVMDSWLYDGDPYVHLEYEKNLEKIKSALTSNYFEDLIE
                     RYMINNTHSSLVSLHPEKGINEKKSAELKKKLEEIKNSFDEKTLNEIIDNCKKLKERQ
                     STPDKKEDLESIPMLSLEDIDKEATKIPTEEKEIDGITTLHHDFHTNKIDYVNFFFNT
                     NSVPQDLIPYVGLLCDILGKCGTENYDYSKLSNAINISTGGISFGAITFANLKKNNEF
                     RPYLEISYKALSSKTNKAIELVSEIVNHTDLDDMDRIMQIIREKRARLEGAIFDSGHR
                     IAMKKVLSYSTNRGAYDEKISGLDYYDFLVNIEKENKKSIISDSLKKVRDLIFNKGNM
                     LISYSGKEEEYENFKEKVKYLISKTSNNNFEKEEYNFELGKKNEGLLTQGNVQYVAKG
                     GNYKTHGYKYSGALSLLESILGFDYLWNAVRVKGGAYGVFSNFRRDGGAYIVSYRDPN
                     IKSTLEAYDNIPKYLNDFEADEREMTKYIIGTIRKYDQPISNGIKGDIAVSYYLSNFT
                     YEDLQKEREEIINADVEKIKSFAPMIKDLMKEDYICVLGNEEKIKENKELFNNIKSVI
                     K"
BASE COUNT         1317 a          300 c          539 g          966 t
ORIGIN      
        1 aaataaataa attaggataa tctttatttt tagaatatag tattttaaaa agactaaatt
       61 tatctaaagg gataaatttt aatctgtaaa tagcaagctt tttaatatag gataattatg
      121 gtacaattat taaaataaac atattaacaa aatacctttt tataggagtg tgaaaaatga
      181 attttaaaga gaataatatt tatagtggat ttaaactttt aaaaatagaa aatttaaatg
      241 aaataggtgg attaggttta aggtttgaac atgaaaaaac taaggctaaa cttataaaaa
      301 tcttaagtga agatgataat aagtgctttg caataggttt tagaacacca tctgaaaata
      361 gtacaggagt tcctcatatt ttagagcatt cagttttatg tggttctaga aaatttaata
      421 ctaaggaacc ctttgtagag cttttaaaag ggtctttaaa tacattctta aatgctatga
      481 catacccaga taaaacaata tatccagtag catcaagaaa tgaaaaagac tttataaatc
      541 ttatggatgt ttacttagat gctgtattat atccaaatat atataagcat aaggaaatat
      601 tcatgcaaga gggatggcat tattatatag aaaataagga agatgaatta aagtataatg
      661 gtgttgttta taatgagatg aaaggggcat actcatctcc agattctata ctttatagaa
      721 agattcctca aacaatatac ccagatactt gttatgcctt atcttcagga ggagatcctg
      781 atgaaatacc aaatttaact tatgaagagt ttgtagaatt tcataagaaa tattatcatc
      841 catcaaactc atatattttc ttatatggta atggagatac tgaaaaagaa ttagaattta
      901 taaatgaaga atatttgaag aattttgaat ataaagagat agattcagaa ataaaagaac
      961 aaaaatcctt tgaaagtatg aaagaagaaa gttttactta tggaatagct gaaagtgaag
     1021 atttaaatca taaaagttat tatagtttaa actttgtaat tggagatgcc acagatggag
     1081 aaaaaggctt agcttttgat gttttagcat atcttctaac aagaagtaca gcagccccat
     1141 taaagaaagc attaatagat gcaggtatag ggaaagctgt atcaggagac tttgataact
     1201 caactaaaca atcagccttt actgttttag ttaagaatgc agagctaaac aaagaagaag
     1261 aatttaaaaa agtagtaatg gatactttaa aggatttggt tgaaaatgga atagataaag
     1321 aacttataga agcttccata aatagagttg aatttgaatt aagagaaggg gattatggtt
     1381 cttatcctaa tgggttaatt tattatttaa aagttatgga tagttggctt tatgatgggg
     1441 atccatatgt tcatttagaa tatgaaaaaa atcttgaaaa aataaaatct gctttaacaa
     1501 gcaattactt tgaagattta attgaaagat atatgataaa taatactcac tcttcacttg
     1561 tttctcttca tcctgaaaaa ggaataaatg agaaaaagtc agctgaatta aagaaaaagt
     1621 tagaagagat taaaaatagt tttgatgaaa agactttaaa tgaaataatt gataattgta
     1681 aaaagttaaa agaaagacaa agtacacctg ataaaaaaga agatttagaa agcattccta
     1741 tgttatcttt agaggatata gataaagaag caactaaaat tcctacagaa gagaaagaga
     1801 tagatggaat tacaacatta caccatgatt tccatactaa taaaatagac tatgttaatt
     1861 tcttctttaa tacaaatagt gttcctcaag atttaatacc ttatgttgga ttgctatgtg
     1921 atatattagg taagtgtgga acagaaaatt atgattattc taagttatca aatgccataa
     1981 atataagcac aggtggaata agctttgggg ctataacttt tgctaattta aagaaaaata
     2041 atgagtttag accatatttg gaaatttcat ataaagcatt aagcagtaag actaataaag
     2101 ctatagaatt agtttctgaa attgtaaatc acactgacct agatgatatg gacagaatta
     2161 tgcaaataat tagagagaag agggctagac tagaaggtgc tatatttgat agtggtcata
     2221 gaatagctat gaaaaaagtt ttatcatact ctacaaatag aggagcttat gatgaaaaaa
     2281 taagtggatt agattattat gattttctag taaatataga gaaggaaaat aaaaaatcaa
     2341 taatatcaga tagcttaaaa aaggtaagag acttaatctt taataaggga aatatgctta
     2401 taagttattc aggaaaagaa gaggaatatg aaaactttaa ggaaaaagta aaatatttaa
     2461 taagcaaaac aagtaataat aattttgaaa aagaagagta taattttgag ttaggaaaga
     2521 aaaatgaagg acttttaact caaggaaatg tacaatatgt agctaagggt ggaaattata
     2581 aaactcatgg atataagtat tctggggccc tatctttatt agaaagtatt ctaggctttg
     2641 actacttatg gaatgccgta agggttaaag gtggagctta tggagtgttc tctaacttta
     2701 gaagagatgg tggagcatat atagtttcat atagagaccc taatataaaa agcactttag
     2761 aagcttatga taatatacct aagtatttaa atgattttga agctgacgaa agagaaatga
     2821 ctaaatacat cataggtaca ataagaaaat atgatcaacc tataagcaat ggaataaaag
     2881 gagatatagc agtttcatat tacttgagta actttactta tgaagatctt caaaaggaaa
     2941 gagaagaaat cataaatgca gatgtagaaa aaattaagag ttttgcacct atgattaaag
     3001 atttaatgaa ggaagattac atctgtgtac taggtaatga agaaaagata aaagaaaata
     3061 aagagttatt taataatatt aaaagtgtaa ttaaatagat tttaaaattc cttaattaaa
     3121 aa
//
LOCUS       DAEVGI010000209         3076 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEVGI010000209 DAEVGI010000000
VERSION     DAEVGI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.211"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..1384)
                     /gene="pepV"
                     /locus_tag="K8M77_002391"
     CDS             complement(<1..1384)
                     /gene="pepV"
                     /locus_tag="K8M77_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBI7086838.1"
                     /translation="MNLISSKIDEMKDDLIQSVQNIIRIKSVEDEPKEGMPFGEGVSK
                     SLEYALEVSKKLGFKVVNLNGHVGYAEYGDGEEYVAALGHLDVVPEGDDWIYPAYGAE
                     IHDGKIYGRGTTDDKGPIMASLYGLKVIKELGLPISKKIRIIFGTNEETGSKDIEYYL
                     EHEKAPVLGFTPDAEFPIINGEKGITIFDIVKTFGEKTTDGDVLVESIKGGIASNVVA
                     SLCETKLRAKEANKVCEEISKFAKESNIKFEVSHKNDEIELKVFGVSAHGSTPEKGIN
                     AIMQTIKILSELNLAQEDIKDFIKFLNDNIGEDVYGEKFGILLQDEASGKLSFNVGVI
                     DLNDKVGRLTLNLRYPVTKTLDDMMIPFNERIKGTCIEIENFKHQKPLYFSPDHPLIK
                     TLKSVYKEETGTEGELISIGGGTYAKEMPNIVAFGPIFPGEPDVIHKPNEYIKIDDLI
                     LISKIYAKALY"
     gene            complement(1574..2884)
                     /locus_tag="K8M77_002392"
     CDS             complement(1574..2884)
                     /locus_tag="K8M77_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HBI7086839.1"
                     /translation="MAQQVISKEQGSIKTLGIFSYFKNGILGLQHLVAMFGATILVPL
                     VTGLDPSVALFTAGIGTLIFHLCTKGKVPVFLGSSFAFIPVIQVIAAKYGDLRYAQGG
                     IFFAGLAYVLISFGIKKIGVDKIKRFLPARVVGPMIMVIGLNLIPNALEMAGVKSLIA
                     GETGAMTSVLIACTTLGLALTISKFGKGFFSQLAIITAVCVGYTLSLILGQVDTSLIQ
                     QSTMVAVPNFTLPKFDFGAIMITLPVILAVSMEHIGDITTNGQVVGKNFIEDPGLNRT
                     ILGDGLATLVASLLGGPANTTYGENTGVLAITKNYNPSILRLTAVFAIILSFIAKFGA
                     VIRTIPQSVMGGISLMLFSMIALVGVKTIKNEGVKLNKTNLILMGSIIFVGLSGLFMS
                     EPIGIEISEAVSISGLSLAALIGVILNLIMTKFESAKSSKEVIA"
BASE COUNT          981 a          574 c          332 g         1189 t
ORIGIN      
        1 gatataaagc ttttgcatat attttactta ttaaaattaa gtcgtctatt ttaatatatt
       61 catttggttt atgaataaca tctggttctc cagggaatat tggtccaaat gctactatat
      121 taggcatttc cttagcatat gttcctcctc caatagaaat taattctcct tctgttcctg
      181 tctcttcttt atatacactc tttaaagtct ttattaaagg atgatctgga gagaaatata
      241 atggtttttg atgtttaaag ttttctattt ctatacaagt accttttatt ctttcattaa
      301 aaggaatcat catatcatct aaagtcttag ttacaggata tcttaagttt aaagtaagcc
      361 tacctacttt atcatttaag tcaattactc caacattaaa gcttaatttt cctgatgctt
      421 catcttgtaa taaaattccg aatttttctc catagacatc ttcacctatg ttatcattta
      481 agaatttaat aaaatctttt atatcttctt gtgctaaatt taactctgat aaaattttaa
      541 tagtttgcat tatagcgttt ataccctttt ctggagtact accatgagct gagacaccaa
      601 atacttttaa ctctatttca tcatttttat gtgatacttc aaactttata ttactttctt
      661 tagcaaattt tgatatttct tcacaaacct tatttgcttc cttagctctt aattttgttt
      721 cacaaagact agcaactaca tttgatgcta ttccaccctt tatactctca actaatacat
      781 ctccatcagt tgttttttct ccaaaagttt taacaatatc aaaaatagtg ataccctttt
      841 ctccatttat tatagggaat tctgcgtcag gagtaaatcc taaaactgga gccttttcat
      901 gttctaaata gtattctata tccttgcttc cagtctcttc attagtacca aatattattc
      961 ttattttctt tgatattggt aatcctaatt cttttataac ttttaatcca tataatgatg
     1021 ccattattgg acctttgtca tcagttgtac ctcttccata tatttttcca tcatgtattt
     1081 ctgctccata tgctggataa atccaatcat caccttctgg aactacatct aaatgtccta
     1141 aagctgctac atattcttct ccatcaccat attcagcata tcctacatgt ccatttaaat
     1201 taactacttt aaatcctaat ttttttgaaa cttccaaggc atattccaaa gatttagaaa
     1261 ctccttctcc aaaaggcata ccttcttttg gttcatcctc aacacttttg attctaataa
     1321 tattttgtac tgattgaatt aaatcatctt tcatttcatc aattttgcta cttattaaat
     1381 tcatttttga cacttcctct catcaacttt aagctaacaa aatattaata tatattatta
     1441 atattttacc acatttaaac aaaattttct aatctatttt attaaattta ctaattaaga
     1501 agttaaagca caaactttac ataattaata ataataaaaa tagcaagcca tatatatggc
     1561 ttgctatttt tatttaagca attacttctt tgctactctt tgctgattca aatttagtca
     1621 ttattaaatt taaaataaca cctattaaag ctgctaaact taagcctgat atgcttacag
     1681 cttcgcttat ttcaattcct ataggctctg acataaataa gccagataaa cctacaaaaa
     1741 ttattgaccc cattaatatt aggttagttt tatttaattt aaccccttca ttttttatag
     1801 ttttaactcc cactaaagca atcattgaga atagcattaa gcttattcct cccattaccg
     1861 attgtggaat tgttcttata accgctccaa acttagctat aaagcttagt attatggcaa
     1921 aaacagctgt taatcttaaa attgatggat tataattttt agttatagct aaaactcctg
     1981 tattttcacc gtaagtagta ttagctggtc cccctaataa agatgcaact aaagttgcca
     2041 atccatctcc taatattgtt ctatttagtc ctggatcttc tataaagttt ttacctacaa
     2101 cttgtccatt agtagttata tctcctatat gctccattga tacagctaaa ataactggta
     2161 aagttatcat tattgctcca aaatcaaatt ttggcaatgt aaagtttggt actgcaacca
     2221 tagtactttg ttgaattaaa cttgtatcaa cttgtcctaa aattaaactt aaagtatatc
     2281 ctacacaaac tgctgttatt attgctaact gtgaaaaaaa tccctttcca aacttactta
     2341 ttgttaatgc taatcctaat gtagtacaag ctattaaaac tgaagtcata gctcctgttt
     2401 ctccagctat taaggatttt accccagcca tttctaaggc attaggtata agatttaacc
     2461 ctataaccat tatcattggg ccaactaccc ttgcaggtaa aaatcttttt atcttatcta
     2521 cacctatttt ctttattcca aaagaaatta atacataagc taatcctgca aagaatatac
     2581 ctccttgtgc atatcttaaa tctccatatt tagctgcaat tacttgtatt acaggtataa
     2641 atgcaaagga tgaccctaaa aatactggaa cttttccttt agtacataag tggaatatta
     2701 aagtacctat ccctgctgtg aataaagcta ctgatggatc taagcctgtt actagtggta
     2761 ctagtattgt agctccaaac atagcaacta aatgttgtaa tcctaaaatt ccatttttaa
     2821 agtaagagaa aattcctaat gttttaatac ttccttgctc tttgcttatt acttgttgtg
     2881 ccataattta agcctcctta ttttttctta aggagaaagc tctgcttctc cttttttcag
     2941 cctcacggga ctgatttaaa aaggtaaaaa taaaacttct taccaaagag attacaggca
     3001 agaagttaat tctttaaact aaataaaatt tttatttata atctaaataa ttaattcttg
     3061 tagcctctct ggacta
//
LOCUS       DAEVGI010000210         3017 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEVGI010000210 DAEVGI010000000
VERSION     DAEVGI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.212"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(187..2277)
                     /gene="fusA"
                     /locus_tag="K8M77_002393"
     CDS             complement(187..2277)
                     /gene="fusA"
                     /locus_tag="K8M77_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBI7086840.1"
                     /translation="MKEYKIENLRNIGIIGHSDSGKTALSEALLYYTKTTDRLGTCED
                     GNTISDYDQEEKKRKISLALSIIPFEYDGTKINILDTPGYFDFVGEQIEGVKAADSAI
                     ITVCGVTGVQVGTEKAWECCEKEKLPRAFFINKLDRENSNFDKVLENIRNIFGNKVIP
                     TQYPLGKEREFKGIVNLITEEAFEYDKKSGKINKIEIPNEVKDKVNEYRTYLMESVAE
                     TDEELLDKYLSEGELTVDEIYKGLSIGFEEGDIAPVMCGSSVSIVGMKSLLDSIKGYF
                     PSPDISKAKVAIDSNNKEILVKSNKDMPFSAFIFKTIADPFVGKLSIFKVQTGKLTTD
                     KIIINSKNNKVEKVSSLCFLRGKSQIQTNKIGAGDIGAITKLQYTSTGDTLCESNFKI
                     AYEGFEFPEAVMTMAVLPKSKGDEEKISQGLSKLLDEDPTFKVSRDQENAETLVSGIG
                     ETHIEVLASKLKSKFGIDVILQDPKIPYRETIKGSSDVQGKHKKQSGGHGQYGDVKIK
                     FEPRRDGELDLEFVDKVVGGVVPRNYIPAVEKGLRDCLKKGVLAGYPVIGIKATLHDG
                     SYHPVDSSEMAFKVAASLAYKKGMENAKPVILEPIMKVEIIIPDEYMGDIISDINKKR
                     GRVIGMEPEGNNEKVIADIPLSEMFKYATDLRSMTQGRGSFSMEFEKYEEVPSTEVDK
                     IIEDAKKIKEAKEA"
     gene            complement(2936..3010)
                     /locus_tag="K8M77_002394"
     tRNA            complement(2936..3010)
                     /locus_tag="K8M77_002394"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2976..2978),aa:Asn,seq:gtt)
BASE COUNT          967 a          562 c          278 g         1210 t
ORIGIN      
        1 attatatatt tttttattca tcagttttat atcgtcaata tactctttct aaattatttt
       61 tttaaacaaa ttttaataat acctttaaca ttattaattt aaccacattt ttttccttgt
      121 atacataaaa agtaccatct ttttaaattt taagatggta ctccccaaat ttctacatat
      181 aattctttaa gcttccttag cttcttttat tttctttgca tcttctatta ttttatctac
      241 ctctgtactt ggaacttcct catatttttc aaattccatg ctaaagcttc ctcttccttg
      301 agtcattgaa cgtaaatcag tagcatactt aaacatttct gataaaggaa tatcagctat
      361 aactttttca ttattacctt ctggttccat tccaattact cttcctcttt tcttatttat
      421 atcactaatt atatcaccca tgtattcatc tggaattatt atttctacct tcataatagg
      481 ctctaatata acaggttttg cattctccat acctttctta tatgctaatg aagcagctac
      541 cttaaatgcc atttctgatg aatctacagg gtgatatgat ccatcatgta atgtagcctt
      601 aattcctatt acaggataac ctgctaaaac ccctttcttt aaacaatctc ttaagccttt
      661 ttctactgct ggtatatagt ttctaggcac aactcctcca acaaccttat ccacaaattc
      721 taaatcaagc tcaccgtctc ttctaggctc aaatttaatt tttacatctc catattgtcc
      781 atggcctcca gattgttttt tatgctttcc ttgtacatca gaactacctt ttatagtttc
      841 tctataagga atcttaggat cttgaagaat tacatcaatt ccaaatttac tctttaattt
      901 actagctaaa acctctatat gtgtctctcc aataccagaa actaaagttt ctgcattttc
      961 ttgatctcta gaaaccttaa atgttggatc ttcatctaga agtttagaaa gtccttgaga
     1021 aattttttct tcatcacctt tactttttgg taatactgcc atagtcataa ctgcctctgg
     1081 gaactcaaat ccttcataag caatcttaaa gttactttca caaagagtat caccagttga
     1141 agtatattga agttttgtaa tagctcctat atctccagca cctattttat ttgtttgtat
     1201 ttgactttta cctcttaaaa agcataagga actaactttt tctaccttat tattttttga
     1261 atttataatt atcttatcag ttgttagctt tcctgtttgc accttaaata ttgaaagctt
     1321 tcctacaaaa ggatcagcaa ttgttttaaa tataaatgca gaaaatggca tatccttatt
     1381 tgacttaact aatatttctt tattattgct atcaatagca actttagctt tgcttatatc
     1441 tggtgatggg aaatatcctt taattgaatc taataagctt ttcataccaa ctattgatac
     1501 tgaacttcca cacataactg gagctatatc tccttcttca aatccaatag aaagtccttt
     1561 atagatttca tcaactgtaa gctctccttc agataaatat ttatcaagta attcctcatc
     1621 agtttcagct acagattcca ttaaataagt tctatactca ttaaccttat cttttacttc
     1681 attaggtatt tctatcttat ttatttttcc tgatttttta tcatactcaa aagcttcctc
     1741 agttataaga tttacaattc ccttaaactc tctttctttt cctaaaggat attgagtagg
     1801 aattactttg tttccaaaaa tatttcttat attttctaat accttatcaa aatttgaatt
     1861 ttctctatct aatttattta taaaaaaggc tcttggtaat ttttcttttt cacagcattc
     1921 ccaggctttt tcagttccaa cttgaactcc agtcactccg catacagtta taattgcaga
     1981 gtcagcagcc ttaacacctt ctatttgctc tccaacgaaa tcaaaataac ctggagtatc
     2041 caatatatta atcttagttc catcatattc aaaaggaata atagataagg ctagtgatat
     2101 ctttcttttt ttctcttctt gatcataatc acttattgta tttccatctt cacaagttcc
     2161 taatctatca gtagtttttg tataataaag taaagcttct gataatgcag ttttacctga
     2221 atcactatgt cctataatcc ctatatttct taaattctca attttatact ccttcataac
     2281 attctgctac accattgata ttgtttacat atcaacatat aagtagcatt caccaccttt
     2341 ctagaaaaat tagtattagt aatctgttta aagcatgcat acatttttta tttaataact
     2401 taattatata aaagcatgta atactttcta cttttatcaa atccccttat gcaataacta
     2461 aaaattaaat ttaaatataa tgtttactta aatatactct tttagttaaa tatcaaaata
     2521 tataaaaact cttaacttta tttttatata taagataaac ttaaactcac tttatactca
     2581 tttgctttct aaaagtgttt ttgaatatgt ctaatattga tttttggcaa taaacatttt
     2641 tataaaaaaa cttatatatc taaatatatt cctttcaaat aaaaaatatt atatttagta
     2701 ttttttatat cttaagatta atgatttatc ctaaaaattc cccatacttt aaaagaaaaa
     2761 tatcaactat attaaactta actttcaagt ttctaatatt aataactata tgttaacact
     2821 ttctttataa tttaataatt caacataaat tattaaattc ctttgaaaaa aacaaaaaag
     2881 agttattaaa aaagacattt atatcttctt taataactct ttttaaataa aaaaatggct
     2941 cctcggagag gactcgaacc tccaacctat cggttaacag ccgagtgctc caccattgag
     3001 ctaccgagga acatttt
//
LOCUS       DAEVGI010000211         3005 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEVGI010000211 DAEVGI010000000
VERSION     DAEVGI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.213"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(223..1395)
                     /locus_tag="K8M77_002395"
     CDS             complement(223..1395)
                     /locus_tag="K8M77_002395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086841.1"
                     /translation="MNKNDLKIMIENGLITNENYEKRGVSIKDFYEISQEIRSNKIDD
                     EFEEAYRSMDKFRREENKKNKIEIIAKDNEQLIYNDKTYKKNGSEYPLTDRLGRGKKE
                     VTHVNSNHKFDIDNLGHKIFYEERIGNVNPEIPIKNENICELLIKSGFKTDLFYYMNV
                     KESQGGAVYFPLLETGKLKISETNSDNLTSSSTLIQGGKVNYIEKKSRPIISEVEFDD
                     MVIINNTKEVNEAIKNDIINQMIDKVSLSRLAVELNKIKALEIEGLNNNIEKVDYLIS
                     EYDSNYLDSDVFVCMNETQYKAINKIRLANGVKIVNIVNGFPYIEGFKIVVIPDLVNI
                     TMLSAKCLGMVIGKLNEGINGHDSQEKRRGIQAYGRIISNDFVIIDPSGIRSISLT"
     gene            complement(1388..1621)
                     /locus_tag="K8M77_002396"
     CDS             complement(1388..1621)
                     /locus_tag="K8M77_002396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086842.1"
                     /translation="MTIRINTKYIEREMLCHYDDSHISRCYKKLAEDTKIQERTLMNN
                     IRNKHFNIDVITEVAFVLKLDVKKVFVKEKNNE"
     gene            2103..2810
                     /locus_tag="K8M77_002397"
     CDS             2103..2810
                     /locus_tag="K8M77_002397"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086843.1"
                     /translation="MLNDIKQWDGKIHIISDERFFALLTNNEYQVYCALKSISYHNNR
                     GKHHLFTDQISFRELSKKLELKVGKNKNGDDTVMTRQTLSRIFKSLEEKKIINKGIIF
                     DDKECYFIITEKDYKFTRIDNELLKVMTKCLKGQVIKTYVYIKANFEYNKLNGKNYTL
                     MNRETIAKAINETNKHTGEIKKKNLDNITAYTTILYNLGLLDISLMTIKSINGAYSSC
                     YRIDAVSDKLKQIPKRK"
BASE COUNT         1134 a          350 c          295 g         1226 t
ORIGIN      
        1 tcatttagtt taattaagtt ctggttctat aaatatacta tttgtaatac taagtatata
       61 aaatactaat ggagtttata taatactaaa tcttatataa aaataataac atataaggaa
      121 atattaataa ctaataatag actattttta tttatgatat tcatactaaa taatagtata
      181 ttagtttatc atataatttt tataactaaa attttttcta aattaagtta atgatattga
      241 tcttattcca gatggatcaa ttataacaaa atcattagaa ataattctgc cataagcttg
      301 aattcctcgt cttttctctt gtgaatcatg gccattaatt ccttcattta atttacctat
      361 aaccatacct aaacattttg cagaaagcat agttatatta actaaatctg gtataactac
      421 aattttaaat ccttctatat aaggaaaacc atttactata tttacaatct taactccatt
      481 tgctaatctt attttattta tagctttata ttgagtttca ttcatacata caaaaacatc
      541 agaatctaag taatttgaat catactctga aattaaataa tctacttttt caatgttatt
      601 atttagacct tctatctcta aagctttaat tttatttaat tctactgcaa gcctagataa
      661 actaacttta tctatcattt gatttataat atcattctta atagcttcat taacttcttt
      721 agtattatta attataacca tatcatcaaa ttctacttct gatattattg gtcttgattt
      781 tttttcaata taattaactt ttccaccttg aattaaagta gaagatgaag ttaaattatc
      841 actgttagtt tctgatattt taagttttcc cgtttctaat aatggaaaat aaacagctcc
      901 accttgagat tctttaacat tcatataata aaataaatct gttttaaaac cagatttaat
      961 aagtagctca caaatatttt catttttaat gggtatttct ggatttacat ttccaattct
     1021 ttcttcatag aatattttat gtcctaaatt atctatatca aatttatgat tactattaac
     1081 atgggtaact tcttttttcc ctctacctaa tctatcagtt aacggatact cactaccatt
     1141 ttttttatag gttttgtcat tataaattaa ttgttcatta tcttttgcta ttatttctat
     1201 tttatttttt ttattttctt ctcttctaaa tttgtccatt gatctataag cttcctcaaa
     1261 ttcatcatct attttatttg atcttatttc ttgagatatt tcataaaaat ctttaattga
     1321 tactccacgt ttttcataat tttcatttgt tataagacca ttttctatca ttatttttaa
     1381 atcgttttta ttcattgttt ttctccttta caaatacttt ttttacatct aacttcaata
     1441 caaatgctac ttctgttata acatcaatat taaaatgttt atttctaatg ttattcatta
     1501 gtgttctttc ttgaatttta gtatcttcag ctaatttttt ataacatctt gaaatatgac
     1561 tatcatcata atgacatagc atttcacgct caatatattt tgtatttatt cgtattgtca
     1621 aattaccact tcctttttaa aattaagtaa aaatttttca tatataaagt acaaaaaaaa
     1681 ataaatagtg tgactaaaat agccacacta tttattaggt aaataatgta tttttactat
     1741 aaaattgttt aaaaaattat tttaggagag aatttaaatt tcttataata aaaaatatat
     1801 actgataaaa gacaaaggtg tataatcttc taccagtatt taaagaagat tattttagaa
     1861 aaatcttata tattaatgat ttaaattgat aatagtgaag aacaatataa ataaaagctg
     1921 tctcaacttc agttgggtta tcttatttat attatataat ataaattaat tgtatattaa
     1981 ttacaatttt tttcaatatt ttttacattt attgaaactt tttttcttta tttatgaact
     2041 taataattgt tttttatata aattaaatat aaataatttt taaagaaaaa ggggaagata
     2101 acatgctgaa tgatataaaa caatgggacg ggaaaattca tattataagt gatgaaagat
     2161 tttttgcttt attaacaaat aatgaatatc aagtatattg tgctttaaaa agtatatctt
     2221 atcataataa tagagggaaa catcatttat ttacagatca gataagtttt agagaattat
     2281 ctaaaaaatt agaattaaaa gttggtaaga ataaaaatgg agatgatact gttatgacaa
     2341 gacaaacact atctaggatt tttaaatctt tagaagaaaa gaaaataata aataagggaa
     2401 tcatttttga tgataaagaa tgttatttta ttattacaga gaaggattat aaatttacta
     2461 gaatagataa tgaattatta aaagttatga caaaatgctt aaaaggacaa gttataaaaa
     2521 cttatgttta tataaaagct aactttgaat ataataaatt aaatggtaaa aattatactt
     2581 taatgaatag agaaactatt gcaaaagcta taaatgaaac aaataaacat actggtgaaa
     2641 taaagaagaa gaatttagat aatattaccg cgtatacaac aatactttat aatcttggat
     2701 tattagatat atcattaatg acaattaaga gtataaatgg agcttatagt tcttgttaca
     2761 gaattgatgc cgtttcagat aaattaaaac agatacctaa aagaaaataa tattaatgag
     2821 gtagttttac ctcatttttt tatttgaaat tttatgtgtt atcaaacgtg tacttactat
     2881 gttatcaaat gtgtacttac tatgttatca aacgtgtact catattaata caattatatt
     2941 aatacaatat tataaatact cttatcttca aaaacctttg gttttgaaga tgtaaagact
     3001 aatca
//
LOCUS       DAEVGI010000212         2996 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEVGI010000212 DAEVGI010000000
VERSION     DAEVGI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.214"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            45..2279
                     /gene="pflB"
                     /locus_tag="K8M77_002398"
     CDS             45..2279
                     /gene="pflB"
                     /locus_tag="K8M77_002398"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI7086844.1"
                     /translation="MFKQWEGFKEGVWQNGIDVRNFIQKNFTAYEGDKSFLEGTTKRT
                     DTVLEKAQKLIIEEIEKGIIDVATDRVSGIDNYEPGYLDKDNEVIVGFQTDAPLKRIV
                     NPFGGMRMVESSLKEYGYELDSSIREHFNAYRKTHNQGVFDAYSAETRAARSAGLLTG
                     LPDAYGRGRIIGDYRRIALYGIDYLIEEKKKDYANISFESEGSVEEYIQLRENVSEQI
                     RALEAIKSMAARYGFDISKPAANAKEAVQFLYFGYLAAVKENNGAAMSLGRTSTFLDI
                     FIERDLKAGTLTELEAQELIDQFIIKLRLVRHLRTPEYNDLFAGDPTWVTEGIGGMGV
                     NGKPLVTKNSFRYLQTLINLGPAPEPNMTVLWSDRLPEGFKKFCAEVSIKTDSIQYEN
                     DELMRPIYGDDYSIACCVSAMQIGKQMQFFGARTNLAKALLYTINGGIDEKSGKVVIK
                     GLEPITDEVLDYDKLRANYDKVLEYVAKLYVDTMNVIHYMHDKYAYEAGQMALHDTHV
                     GRLMAFGIAGLSVVADSLSAVKFAKVKPIRENGIAVDFEIEGDFPKYGNDDDRVDDLA
                     VEIVRKFSSELKKHPLYRGAKHTLSILTITSNVVYGKKTGSTPDGRKAGEAFAPGANP
                     MHGRDNSGALASLNSVAKIPYCEVCEDGVSNTFSIVPDALGKEEDSRIDNLVAIMDGY
                     FKQGAHHLNVNVFNRETLMDAMEHPEKYPTLTIRVSGYAVNFNRLTRNQQLEVISRTF
                     HDSL"
     gene            2359..>2996
                     /gene="pflA"
                     /locus_tag="K8M77_002399"
     CDS             2359..>2996
                     /gene="pflA"
                     /locus_tag="K8M77_002399"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBI7086845.1"
                     /translation="MVKGRIHSLETMGLVDGPGIRFVVFMQGCGIRCAFCHNPDTWCK
                     DKGTEYTPEELVNKIKRFKTYFNASGGGVTFSGGEPLLQPEFLLECLKLCKKEGIHTT
                     LDTAGVGLGNYEEILEYVDLILFDVKETDPEKYKNLVRVPIDKSLEFLKVAQSMNKKM
                     WIRHVVVPGYTDNKEDLMRIKNFVDGLNNIEKVELLPYHVLGVNKYEGLNIP"
BASE COUNT         1117 a          379 c          601 g          899 t
ORIGIN      
        1 tttaaattga taattatttt caattaaaag agggggaaga ccatatgttt aaacaatggg
       61 aaggctttaa agaaggagta tggcaaaacg ggatagatgt tagaaacttc atacaaaaga
      121 actttactgc atatgaggga gacaagagtt tcttagaagg aacaacaaaa agaactgata
      181 cagttttaga aaaagctcaa aagttaatta tagaagaaat agaaaaaggc attatagatg
      241 ttgctacaga tagagtatct ggaatagaca attatgagcc tggatattta gataaagata
      301 acgaagttat cgttggtttt caaacagatg caccacttaa aagaatagtt aatcctttcg
      361 gtggaatgag aatggttgaa agttctttaa aagaatacgg ctatgaatta gatagcagta
      421 taagagaaca ctttaatgct tatagaaaaa ctcataacca aggtgtattc gatgcatact
      481 cagctgaaac aagagctgca agatctgctg gtcttttaac tggtcttcca gatgcttatg
      541 gtagaggaag aattataggt gactatagaa gaattgccct ttacggtata gattatctaa
      601 tagaagaaaa gaaaaaagat tatgctaata tatcatttga aagcgaaggc tcagtagaag
      661 aatatattca attaagagag aatgtttcag agcaaataag agctttagaa gcaataaaat
      721 caatggctgc aagatatggt tttgatatat ctaaaccagc tgcaaatgct aaggaagcag
      781 ttcaattctt atactttgga tacttagctg cagttaaaga aaacaatgga gcagcaatgt
      841 ctctaggaag aacttctact ttcttagata ttttcataga aagagatctt aaggcaggaa
      901 ctttaactga gttagaagct caagaattaa tagatcaatt tataataaaa ttaagattag
      961 ttagacactt aagaactcct gagtataatg atttatttgc tggagaccca acttgggtaa
     1021 cagaaggtat tggtggtatg ggtgtaaatg gtaagccatt agtaactaaa aactcattta
     1081 gatatcttca aactttaata aacttaggac ctgctccaga accaaatatg actgttttat
     1141 ggtcagatag attaccagaa ggattcaaaa aattctgtgc tgaggtatca attaaaactg
     1201 actcaattca atatgaaaat gatgagttaa tgagacctat atacggagat gattactcaa
     1261 tagcttgctg tgtatcagca atgcaaattg gtaagcaaat gcaattcttc ggagctagaa
     1321 ctaacttagc taaagcttta ctttacacta taaatggtgg tattgatgaa aaatcaggta
     1381 aagttgttat taaaggttta gagccaataa cagacgaggt tttagattat gataaattaa
     1441 gagctaacta tgataaagtt ttagagtacg ttgctaagct ttatgtagat acaatgaatg
     1501 ttatccacta catgcatgat aaatatgctt atgaagctgg acaaatggct ttacatgata
     1561 ctcatgtagg aagattaatg gcatttggta tagctggttt atcagttgta gctgactcat
     1621 taagcgcagt taaattcgct aaagttaaac ctataagaga aaatggaata gctgtagatt
     1681 ttgaaatcga aggtgacttc cctaaatacg gaaatgatga tgacagagtt gacgatttag
     1741 ctgtagaaat agttagaaag ttctcaagtg agcttaagaa acatccatta tatagaggtg
     1801 ctaagcacac attatcaatc ttaactatta cttcaaacgt agtttatggt aagaaaacag
     1861 gttcaactcc agatggaaga aaagctggtg aagcatttgc tccaggagct aacccaatgc
     1921 atggaagaga taacagtgga gctttagcta gcttaaactc agttgctaaa attccatact
     1981 gtgaagtttg tgaagatggt gtatcaaaca cattctcaat cgttccagat gctttaggaa
     2041 aagaagaaga ttctagaata gataacttag ttgctattat ggatggttac ttcaaacaag
     2101 gagctcatca tctaaatgtt aatgtattta acagagaaac tttaatggac gctatggagc
     2161 atcctgaaaa gtatccaaca ttaacaatca gagtatcagg ttatgctgtt aatttcaata
     2221 gattaactag aaatcaacaa ttagaggtta taagcagaac attccacgat tctctataat
     2281 cttatataat aagagtgtat aaggtgaagg ctttatacac tcttttttga aatgtttaca
     2341 gtaaggaggg acagcattat ggttaaagga agaattcatt cattagaaac tatgggctta
     2401 gtagatggac ctggtataag atttgtagtt tttatgcaag gctgtggaat aagatgtgct
     2461 ttttgtcata atccagatac ttggtgtaag gataaaggga ctgaatatac tccagaggag
     2521 cttgtaaata agataaaaag atttaaaaca tattttaatg catcaggtgg tggtgttact
     2581 ttctcaggtg gagagcctct tcttcaacca gaatttttat tagaatgttt aaaattatgt
     2641 aagaaggaag gaatacatac cacattagat actgcaggtg tgggattagg aaattacgaa
     2701 gaaattcttg aatatgttga ccttatatta tttgatgtaa aggaaactga tcctgaaaaa
     2761 tataaaaatt tagtaagagt tccaatagat aaatccttag agtttctaaa ggtagctcag
     2821 tccatgaata aaaaaatgtg gataagacat gttgtagttc ctggatatac agataataaa
     2881 gaagatttaa tgagaattaa gaattttgta gatggtctta ataatataga aaaggttgaa
     2941 cttttacctt atcatgtttt aggtgtaaat aaatatgaag ggcttaatat tccata
//
LOCUS       DAEVGI010000213         2977 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEVGI010000213 DAEVGI010000000
VERSION     DAEVGI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.215"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(196..1599)
                     /gene="pyk"
                     /locus_tag="K8M77_002400"
     CDS             complement(196..1599)
                     /gene="pyk"
                     /locus_tag="K8M77_002400"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI7086846.1"
                     /translation="MRRTKLVCTIGPASENEEILTKIIEAGMNASRHNFSHGDHEEHK
                     GRMVKVREISKKLGKEVAILLDTKGPEIRTGKFEPSKVELTAGTEFTIYAGAEDIIGD
                     TTKCSVTYAGLAKDVKAGDTILIDDGLVGLEVVSVEGNAVKCVVRNTGLVGTHKGVNV
                     PGVSIKLPAMTDKDRADLIFGCEMGVNMVAASFIRKAEDVKAIREVLVANGGADIQIF
                     SKIENQEGVDNIDAIIEASDGIMVARGDLGVEIPMEDVPSVQKMIIEKCNNAGKPVIT
                     ATQMLDSMMRNPRPTRAEVSDVTNAILDGTDAIMLSGESANGSWPVEAVETMVKIATK
                     SEEMLSYELASSKAKKHIPAVPGVISRAACNAAHELKSAAIVSLTQSGATAKRISQCR
                     PDAPIVTVTPNERVAKKVALCFGVYPVVAENATMENAVEIAKNAGFVKANDTAVVVAG
                     VPANEGNTNIVKVEVVK"
     gene            complement(1692..2651)
                     /gene="pfkA"
                     /locus_tag="K8M77_002401"
     CDS             complement(1692..2651)
                     /gene="pfkA"
                     /locus_tag="K8M77_002401"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI7086847.1"
                     /translation="MKKIAVLTSGGDAPGMNAALRAVTRMALHHGLEVMGVQRGYAGL
                     INGELFKMDRKSVSEIINRGGTILRTARCLEFKQEEVREKAAQILKAYGVEALVVIGG
                     DGSFMGAKLLSKLGVKTVGLPGTIDNDLSYTDYTIGFDTALNTVVDAINKLRDTSTSH
                     ERVSIVEVMGRNCGDLALYAGIAGGAEAIIVPEMPFDKDELIKTILEGRTNGKTHSII
                     VVAEGVGGSAELAKEIESVTGIETRATILGHIQRGGSPTADDIVLASRMGAKAVEVLL
                     EGKTSKVIGIKENRVFDMDIDEALDIERSFDESLYEMASAINK"
BASE COUNT          869 a          592 c          377 g         1139 t
ORIGIN      
        1 cccatttaag attagaatat tttatctaac tttataacct ttcaaaattt atattttaat
       61 taataaaaat ataaaccaaa aaatttttat attaaacaac aaaaacacac tctcataaat
      121 atttaaataa tgagggtatg ttttcataaa aaaataggta gaatactcta cctattttta
      181 taatcagtta taaaattatt ttacaacttc aacttttact atgttagtgt ttccttcgtt
      241 agctggtaca cctgcaacaa ctacagcagt gtcattagct ttaacgaatc cagcattttt
      301 agctatttca acagcgtttt ccattgtagc attttcagca acaactgggt aaactccgaa
      361 gcataaagca actttcttag caactctttc atttggagtt acagtaacga ttggtgcatc
      421 tggtctgcat tgagatattc tcttagcagt agctcctgat tgagttaatg aaactatagc
      481 agctgatttt aactcgtgtg cagcgttaca agcagctctt gagataactc ctggtacagc
      541 tggtatgtgt tttttagctt ttgatgaagc taattcgtag cttaacattt cttctgattt
      601 agtagctatt ttaaccatag tttcaacagc ttctactggc cagcttccgt tagctgactc
      661 accacttaac atgatagcat cagtaccatc taagatagcg ttagttacgt ctgaaacctc
      721 agctcttgtt ggtcttgggt ttctcatcat tgagtctaac atttgagtag cagttataac
      781 tggttttcca gcattgttac atttttcgat gatcattttt tgaactgaag gaacgtcttc
      841 cattggtatt tcaacaccaa ggtctcctct agctaccatg ataccgtctg aagcttcgat
      901 gatagcatct atgttatcaa caccttcttg gttttcgatt tttgagaata tttgtatatc
      961 agctccaccg ttagcaacta atacttctct tatagcttta acgtcttctg cttttcttat
     1021 gaatgaagca gcaaccatgt taacgcccat ttcacaacca aatattaagt cagctctatc
     1081 tttatcagtc atagctggta attttattga tactccagga acgttaactc ctttgtgagt
     1141 tccaactaat ccagtgtttc ttactacaca tttaacagcg tttccttcta cagatacaac
     1201 ttctaatcca actaaaccgt cgtctattaa gatagtatct ccagctttaa cgtctttagc
     1261 taatccagca tatgtaactg aacacttagt agtgtctcct attatatctt ctgctccagc
     1321 atatatagta aattcagttc cagcagttaa ttcaactttg cttggctcga atttcccagt
     1381 tcttatttca ggtcctttag tatctaataa gatagctact tctttaccta atttttttga
     1441 tatttctctt actttaacca ttcttccttt atgttcttca tggtcaccat gtgagaaatt
     1501 gtgtcttgaa gcgttcatac ctgcttcgat tatttttgtt aagatttctt cgttttcact
     1561 agctggtccg atagtgcata ctaattttgt tcttctcatt taaaaacact ccctcgtaaa
     1621 aaaattaacc ctaagtttaa aatatataat aaataataaa tttaaaaaat ttattatgtc
     1681 attaactata attatttgtt tatagcgcta gccatttcgt aaagactttc gtcaaagctt
     1741 ctttctatat ctaaagcttc atctatatcc atatcgaata ctctattttc tttaattcct
     1801 ataacttttg aagtttttcc ttctaataaa acttctacag ctttagctcc cattcttgat
     1861 gctaatacta tatcatcagc agttggtgat ccacctcttt ggatgtgacc taaaattgta
     1921 gctctagttt ctataccagt tacagattct atttctttag ctaattcagc tgatcctcca
     1981 actccttcag caacaactat tatgctgtga gtttttccat ttgttctacc ttctaagata
     2041 gttttgatta attcatcttt atcaaatggc atttctggaa ctattatagc ttctgctcca
     2101 cctgcgatac cagcatataa agcaaggtct ccacagtttc ttcccataac ttctacgata
     2161 gatactcttt cgtgtgaagt tgatgtatct cttaatttgt ttatagcatc tactactgtg
     2221 tttaatgcag tatcaaatcc tatagtgtaa tctgtgtaag ataagtcgtt atcgattgtt
     2281 cctggtaatc caacagtttt aactcctaat tttgaaagaa gtttagctcc catgaatgat
     2341 ccatctccac ctataactac taaagcttca acaccgtaag cttttaatat ttgtgcagct
     2401 ttttctctta cttcttcttg tttgaattct aaacatctag cagttcttaa gattgtacca
     2461 cctctgttta tgatttctga aacagatttt ctatccattt tgaatagttc tccatttatt
     2521 aatccagcgt atcctctttg aactcccatt acttcaagtc catggtgtaa agccattctt
     2581 gtaactgctc ttaaggcagc gttcattcct ggtgcatctc caccacttgt taaaacagca
     2641 atctttttca taatattttc ctcctctaca ccactcggga caaatttagt cccaacagtg
     2701 ccaaatatta cccacatttt taagatatta tttatatatg ttaaatgttt atcaatttta
     2761 aactatttta aatttaatcc taatttaaat tttatcaaaa atttaaatac cctatgcaac
     2821 tttattaata ctattctacc acaattaatt gtacttaaag tagtgcttat tttcaacctt
     2881 tttgtgtaga attaaagaaa aaaacctcga tatatgataa ttttttcaat tattattatg
     2941 ctctaaaatc tttttaatat ttaattaaat ataaaaa
//
LOCUS       DAEVGI010000214         2939 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEVGI010000214 DAEVGI010000000
VERSION     DAEVGI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.216"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(12..350)
                     /locus_tag="K8M77_002402"
     CDS             complement(12..350)
                     /locus_tag="K8M77_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBI7086848.1"
                     /translation="MDKLPNCPKCNSEYTYEDGSLLVCPECTYEWTPGSENSDEDVLV
                     VKDSNGNILQDGDTVTIIKDLKVKGASSALKKGTKVKNIRLVEGDHNIDCKIDGFGAM
                     SLKSEFVKKI"
     gene            complement(783..1814)
                     /locus_tag="K8M77_002403"
     CDS             complement(783..1814)
                     /locus_tag="K8M77_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI7086849.1"
                     /translation="MSKYAILANPGHNRIYFDTALKIAVSELQAIADAYDMEIKEIEE
                     GNLGLPASICFSTNRELEEEDFKALSFSSIYYALFEIVEGNLLRPISVPDFHTFPESM
                     VQILKYNGKTNEQFTRLMVNLALSACKTGSKKITLLDPMCGKGTTLYEGFIRGFDVKG
                     IEINEQWTQEIQTYVVRFLKEGKFKHKVEKSKVSDSKKKKIANVFNLTAAAEKNAFNS
                     GEIQSFQLFNSDTRKADLLIKKKSCDIIISDLPYGVQHGSKNDRNSLMERSPLGLLKE
                     AIPAWHNALKTKGSLVLSYNEFTMKYDEVAIVLEKNGFKVLDEKPYHNYLHRVDQSIN
                     RNLIVAVKA"
     gene            complement(1940..2308)
                     /locus_tag="K8M77_002404"
     CDS             complement(1940..2308)
                     /locus_tag="K8M77_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI7086850.1"
                     /translation="MGNENYRKQNSDGSILKWIGRFILVAIVLVITSFVTPGFSIHGL
                     WSFLLAAVVISVLDYLVELFMGVDASPFGKGIKGFIIAAIIIYLTQFLVPNMGVSIIG
                     ALLAAVVIGIIDAVIPNKIM"
     gene            complement(2544..>2939)
                     /locus_tag="K8M77_002405"
     CDS             complement(2544..>2939)
                     /locus_tag="K8M77_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBI7086851.1"
                     /translation="VKVTFAKVESSDNITEDQKPENNQKPENNQKPENNQKPEENQKP
                     ENNQKPEENQKPEDNQKPEDNQKPEDNQKPENNQKPEDNQKPNEEQKPGNDQIKEELP
                     QTGSNVNSASMGILGLTSLLGGLFIKKNR"
BASE COUNT          932 a          476 c          304 g         1227 t
ORIGIN      
        1 cttcttttta attaaatttt tttaacaaat tctgatttta agctcatagc tccaaatcca
       61 tctattttac aatctatgtt gtgatcacct tcaactagac gtatgttctt aacttttgta
      121 ccctttttta aagctgaaga agcacctttt actttaaggt cttttattat agttacagta
      181 tctccatctt gaagtatatt tccgtttgag tctttaacta ctaatacatc ctcatctgaa
      241 ttttcactgc ctggagtcca ctcataagta cattctgggc aaactaaaag acttccatct
      301 tcataagtgt attctgaatt acatttaggg caatttggta atttatccat tatacatatc
      361 ctccgttcat taactaaatt ctccttagta taaatattga tttatacata tagggattta
      421 cttataggct tatataatat catagtctaa taataatgac aaattttaag ctttttaaag
      481 ataaattgct ataaccttct aaaatattga ataataataa aatatattat aatttgctaa
      541 atttcaaagt ttttattctt attttaatat cctaataaaa tacttccatc ttagctaact
      601 ataaaaattt ctgagttatt ctatctcaat tctttttaac aactacttga taaaccttag
      661 ctctatttat atctatatat tctttaaaat tttcatatgt attattagga ttatttctat
      721 ttctttttta caagttcaat ttgtaaaaaa gaaaggatta cttaattagt aatcctctat
      781 ttttaagcct ttacagctac tattaaattt ctatttattg attgatctac tctatgtaaa
      841 taattatgat aaggtttttc atcaagaact ttaaatccat ttttctctaa aacaatagct
      901 acctcatcat atttcatagt aaattcattg tatgaaagta ctaaacttcc tttagttttt
      961 aatgcattat gccatgctgg aattgcttct tttaaaagcc ctagtggact tctttccata
     1021 agagaatttc tatcattctt acttccatgc tgaaccccat aaggtaaatc agatattata
     1081 atatcacaag attttttctt aataagaaga tctgcttttc tagtatctga attaaataac
     1141 tggaatgatt gaatctctcc tgaattaaaa gcattttttt ctgctgcggc agttaagtta
     1201 aatacattag ctattttctt ctttttagaa tctgacacct tagatttttc aactttatgc
     1261 ttaaactttc cttcttttaa aaatctaaca acataagttt gaatctcttg agtccattgc
     1321 tcatttatct ctattccttt aacatcaaat cctcttatga atccttcata taaagttgta
     1381 cctttaccac acataggatc taataaagtt atctttttac ttcctgtttt acaagcactt
     1441 aaagctaaat ttaccataag tctagtaaat tgttcatttg tctttccatt atattttaat
     1501 atttgaacca tactttcagg gaatgtatga aaatctggga cactaatagg tcttaaaaga
     1561 tttccttcta ctatttcaaa taaggcataa taaattgatg aaaaacttag tgctttaaag
     1621 tcttcttcct caagctctct attagtgcta aagcatattg atgctggaag acctaaattt
     1681 ccttcttcaa tctcctttat ctccatatca taagcatctg caattgcttg taattctgaa
     1741 actgcaatct taagcgctgt atcaaaataa atacgattat gacctggatt tgcaagtatc
     1801 gcatatttgc tcataaaaaa atcctcactt attcttatct tttaattcta ttttttatcc
     1861 ataatattat aacatcacat actttaaata ttaataaaaa atagagtact tttattaaaa
     1921 gcactctatt ttaaaagttt tacataattt tatttggaat tacagcatct attattccta
     1981 taactacagc agctagtaat gctcctataa ttgaaactcc catgtttgga actaagaatt
     2041 gagttaaata tattattatt gcagctatta taaagccctt tatccctttt ccaaatggcg
     2101 atgcatcaac acccataaat aattctacta aataatctaa aactgatatt acgacagcag
     2161 ctaataaaaa tgaccataat ccatgtattg aaaatcccgg agttacaaat gatgttatta
     2221 ctaatactat ggcaactaaa ataaatcttc ctatccattt taatatacta ccatcactat
     2281 tttgttttct ataattttca tttcccataa gtttgcctcc ttttatagat tgatacattg
     2341 taaagtacta tttattttta aatattaata acattcttta cacttatatg ttgaccacta
     2401 tatcaaataa tattccttta aaaataaatt taatatagtt tataataaaa ttataaaaat
     2461 ctaataacta cttgttagca aatttaaaaa aataaggaag aagttaaatt aacttcttcc
     2521 ttaaatttaa tataataatc atcttatcta tttttcttta tgaataatcc tccaagtaaa
     2581 gatgttaatc ctagtattcc catagaagct gaattaacat ttgaacctgt ttgaggtaat
     2641 tcttctttta tttgatcatt tcctggtttt tgttcttcat ttggtttttg attatcttct
     2701 ggtttttgat tattctctgg tttttgatta tcttctggtt tttgattatc ctctggtttt
     2761 tgattatcct ctggtttttg gttttcttct ggtttttgat tattctctgg tttttggttt
     2821 tcttctggtt tttgattatt ttctggtttt tgattattct ctggtttttg attattttct
     2881 ggtttttgat cttctgtaat attatcagag ctttcaactt tagcaaatgt tacttttac
//
LOCUS       DAEVGI010000215         2933 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEVGI010000215 DAEVGI010000000
VERSION     DAEVGI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.217"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(192..1061)
                     /locus_tag="K8M77_002406"
     CDS             complement(192..1061)
                     /locus_tag="K8M77_002406"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBI7086852.1"
                     /translation="MRINRNDLCWCNSGKKYKKCHMEFDERIENLAREGFEVPTRDII
                     KNEEQIEGIRKSAEINNAVLDLVAEKIKAGMSTEEINKIVHDYTVSRGAIPAPLNYGG
                     FPKSVCTSINDEVCHGIPDENIILKEGDIINVDVSTIYNGYYSDASRMFIIGEASENA
                     KRLVKIAKECLEKGIEAVKPWGFLGDIGAAIQEHAEKNGYSVVRDFGGHGVGLKFHED
                     PFVYHYGDAGEGMVLVPGMIFTIEPMINEGKYDVFVDVENDWTALTMDGSLSAQWEHT
                     ILVTEDGIEIIAK"
     gene            complement(1409..2005)
                     /locus_tag="K8M77_002407"
     CDS             complement(1409..2005)
                     /locus_tag="K8M77_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBI7086853.1"
                     /translation="MLYKTHLEIGENTFNTIEGRGRNLIDKKSFLKGNILPDISPKYR
                     LKKHGRKQFEEIIDEKVKALSRLSSDEIINHLGKEKFSIELGVVCHFLCDFFSLPHDE
                     EWSFKESVTLKHVLYEKKLHKVFSKDIIIKDSYKILGNSDYDVFFKNALKKYRNVYGY
                     RNDLFFAMYLNNSVVRYVLESIEENDKINLSKEKDIAF"
     gene            2103..>2933
                     /locus_tag="K8M77_002408"
     CDS             2103..>2933
                     /locus_tag="K8M77_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086854.1"
                     /translation="MTLKNKNNLIKHLSFITIILISFILIFTFKDNSTKSAINENTIK
                     ETIKSDLNGDGKEDCLYIELESENNYIINATINEKSYELIPNKAINSLGKFSPNRPIT
                     LNLLDLDRNNIKEIIVQSSEENSSIQHLFKWTGNGFEDIFYSTNNILGVVDSNNGKTP
                     KILSFSLGDSKENIQKYMLLNKKFKNISYDTVEPTGLYSIISFIDIISLNYEISELPN
                     IFATYISKEDLSQIWRLEKESYYYDFQDAFFMDIAWNNTGEATNCSWTLNFNKIPKEN
                     P"
BASE COUNT         1032 a          488 c          265 g         1148 t
ORIGIN      
        1 gcttacacag gaaaattatt atattgattt attaaagtca attgaacctt atttagattt
       61 attgaaaaat cttaatatac caattcctaa atgtccagaa tataaagtta aatcaccagc
      121 tgtactaata acaccaataa gcatgtattt ttaaagcctt ctaaaattaa ttagaaggct
      181 ttaaattttt attatttagc tattatttca attccatcct ctgtaactag tattgtgtgc
      241 tcccattgag ctgaaagcga tccatccata gttaaggcag tccaatcatt ttctacatct
      301 acaaaaacat catatttccc ttcatttatc ataggctcta ttgtaaatat catacctggt
      361 actaatacca taccctctcc agcatctcca tagtgatata caaatggatc ctcatggaat
      421 tttaatccta ctccatgacc tccaaaatct cttacaactg aatatccatt cttttctgca
      481 tgctcttgaa ttgctgcccc tatatctcct aagaatcccc aaggtttaac tgcttcaata
      541 cctttttcta agcattcttt agctatttta actaatctct ttgcattttc actagcttct
      601 ccaataataa acattcttga ggcatctgaa taatatccat tatatatagt agatacatca
      661 acgtttatta tatctccttc tttaaggatt atattttcat caggaattcc atgacaaacc
      721 tcatcattta tagaagtaca aacactttta gggaatccac catagttaag tggtgctgga
      781 atagcccctc tacttacagt ataatcatgt actattttat ttatctcttc agtactcata
      841 cctgctttta ttttctctgc aactaaatct aaaacagcat tgtttatttc tgcacttttt
      901 cttatacctt ctatttgttc ttcatttttt attatatctc tagtaggaac ttcaaatcct
      961 tctctagcta aattttctat tctctcatca aattccatat gacatttttt atatttctta
     1021 ccactgttac accagcataa atcatttcta tttattctca ttttcctgtc tccttataaa
     1081 agctttaaat acaccttaaa gtatatatct atacttttaa gtatatacta agttatactt
     1141 ttttcaagtt tacacattat taataagatt ataaaaaaag atataagatt tatgtactat
     1201 atactatctt atatctataa ttctaaaaac aatataaatt ttactctctt ttatctataa
     1261 aaagagctat atttcctctt attatactca ttgtaattca ttaaattata tataactccc
     1321 tttatattaa tcctctttgc aaatattatc atattaagtt tctcccattt atccttttta
     1381 taataatatg taatatttgc ttcaattatt aaaaagcaat atctttttct tttgaaagat
     1441 ttattttatc attttcttct atggattcta aaacatatct aacaactgaa ttatttaaat
     1501 acattgcaaa aaacagatca tttctatatc catatacatt tctatatttc tttaaagcat
     1561 ttttaaaaaa gacatcataa tctgagtttc ctagtatttt ataactatct tttattatta
     1621 tatctttact aaaaacctta tgtaactttt tctcatataa cacatgcttt aaggttacac
     1681 tctccttaaa actccattcc tcatcatgag gcaaagaaaa aaagtcacat aaaaaatgac
     1741 acactactcc taactctata ctaaattttt cttttcctaa atgatttatt atttcatcac
     1801 tacttaacct acttagcgct tttacctttt catcaataat ctcttcaaac tgttttcttc
     1861 catgtttttt caatctatat ttaggtgaaa tatctggaag aatatttcct tttaaaaaac
     1921 tttttttatc aattaaattt cttcctcttc cttcaatagt attaaatgta ttttctccta
     1981 tttcaagatg agttttataa agcatattaa tcaccacctt attatatttt gttcaaagta
     2041 caaaaaaaaa ttacaataat aaaaaataga ttatatgcat ataatctatt tgaggtgatt
     2101 ttatgacttt aaaaaacaaa aataatttaa taaaacatct tagttttatt actattattt
     2161 taatttcttt tattttaata tttaccttta aagataattc tactaaatct gctattaatg
     2221 aaaatactat taaagaaact ataaagtctg atttaaatgg tgatgggaaa gaagattgtt
     2281 tatatataga gttagagagt gaaaataatt atataataaa tgcaactatt aatgaaaaaa
     2341 gttatgagtt aattccaaac aaagcaatta attctctagg taaattttcc cctaatcgtc
     2401 ctataacttt aaacttatta gatttagaca gaaataatat taaagagata attgttcaat
     2461 cttctgaaga aaatagctct atacagcatt tatttaagtg gactggaaat ggctttgaag
     2521 atatatttta ctctacaaac aatatattag gagttgtaga ttcaaataat gggaaaaccc
     2581 ctaaaatact ttctttttcc ttaggtgata gtaaagaaaa tattcaaaaa tatatgcttt
     2641 taaataaaaa atttaaaaac atatcctatg atacagttga acctactgga ctatattcta
     2701 taatttcttt tatagacata atatctttaa attatgaaat atctgaactt ccaaatatat
     2761 ttgcaactta tatttctaaa gaagatctat ctcaaatatg gagattagaa aaagagtctt
     2821 attattatga ctttcaagat gcttttttta tggatatagc ttggaataac actggagaag
     2881 ccactaattg ctcttggaca ttaaacttta ataaaatccc aaaagaaaac cct
//
LOCUS       DAEVGI010000216         2917 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEVGI010000216 DAEVGI010000000
VERSION     DAEVGI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.218"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(157..651)
                     /locus_tag="K8M77_002409"
     CDS             complement(157..651)
                     /locus_tag="K8M77_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086855.1"
                     /translation="MEKFYELSISGEESKLHKMIVPAMIISLFLAAGIFIFTGLIPAL
                     IFLILICLPLFIAKRLTMVEYDYEFTDGELEISAIYERVRRKVKGNINLRKIEIVAPL
                     GNSELKRYSNVKVKKCYNNIQEGKKVYVIVVRGETKMVAYEVLIDQKMLDLFFFSNPQ
                     KVIR"
     gene            885..1910
                     /locus_tag="K8M77_002410"
     CDS             885..1910
                     /locus_tag="K8M77_002410"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBI7086856.1"
                     /translation="MDKKTAIDWNNLGFSYMKTDYRYISHYKDGKWDEGKLVTDNKLS
                     ISEASTALHYGQQCFEGLKAYRTKDGKIQLFRVDENAKRMNKSCDKLLMPEIPVEKFI
                     DACMQVVKANERFVPPYGTGATLYIRPFMIGVGDNIGVKSAPEFIFSVFCLPVGAYFK
                     GGMKPVNFMIADYDRAAPKGTGAAKVGGNYAASLKAHEIAAKKGFADCIYLDPATHTK
                     IEEVGAANFFGITKKGEFVTPYSESILPSITKYSLMQIAKDYLKMPVSERDVLIDNLD
                     EFAEAGACGTAAVITPIGGIEYKNKLHVFHSETEVGPITKKLYDLLSGMQFGDVEAPE
                     GWIFEVK"
     gene            complement(1954..2367)
                     /locus_tag="K8M77_002411"
     CDS             complement(1954..2367)
                     /locus_tag="K8M77_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086857.1"
                     /translation="MKLLNKLVITFFITILVTVCSYFFMPIKLEAAFNMLIENPEYIV
                     VTEKKNDSEFNSYKIEDENDIANIYDDIKEIKIVKPFKNPVISNEYNIYMGDQYISLS
                     LSEEFIKVNDETFRIIDSGDKYKELLREIKENFIK"
     gene            complement(2390..>2917)
                     /locus_tag="K8M77_002412"
     CDS             complement(2390..>2917)
                     /locus_tag="K8M77_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086858.1"
                     /translation="KSISEDKFKNKIKIRFNNILDGFDRYSNGLLEYNGDNDSFKIKE
                     ECFINFFNEALELNKGKVIIDLYIKDLENESLARLLEGLDERDKNILIDINKHEIKSV
                     YFELHNKDLMSFITRLNTRELFFCTIYFMEKPMTIWGNYNLSFPMFFEENDMLEIYID
                     LAKKHNLDVRGIVLK"
BASE COUNT         1028 a          458 c          340 g         1091 t
ORIGIN      
        1 agactgttaa cttcttagga atacaatctc acaaagttcc actctataag ttcaagaatc
       61 caaattaaaa atttgggctt tcacttaaaa tctagtaatt ctcgaaatat gtttgtaagt
      121 tttaaacaag ttattttgat acacgccctt tttttattat ctaataacct tttgaggatt
      181 tgaaaaaaag aataaatcaa gcattttttg atctattaaa acttcatatg caaccatttt
      241 agtttcccct ctaaccacaa ttacataaac cttttttcct tcttgaatat tgttataaca
      301 tttctttact tttacattag aatatctctt taattcacta tttcctaaag gtgcaacaat
      361 ttcaatcttt cttaaattta tgttaccctt tacttttctt ctaactctct cataaatagc
      421 gcttatttca agttctccat cagtaaattc atagtcatat tcaaccattg ttaacctttt
      481 agctataaaa agaggcaaac atattaatat taaaaatatc aaagcaggta ttagccctgt
      541 aaaaataaat attcctgctg ctaaaaacaa tgaaataatc atagctggta ctatcatctt
      601 atgtagcttt gattcttcgc cacttatact taattcataa aacttctcca tacccgtctc
      661 cctttatcat ttactttttc tttcattata ccatacattt cttttcaaat ttatatacta
      721 aacctagtaa taaatattaa ttttttgtat tattttaata tttatatcat ttaaacttaa
      781 cattaaaata attttaattt gcaattttta aattttaatc tttcattttt taaaataaaa
      841 tgataaaatt aaaaagtatt aaattttgat aggagtgtta aactatggat aaaaagacag
      901 ctattgattg gaataatcta ggcttctctt atatgaaaac agattatcgt tacatatctc
      961 actataaaga tggtaaatgg gatgaaggaa aattagttac agacaacaaa ttaagcataa
     1021 gtgaagcttc aactgccctt cactatggcc aacaatgttt tgaaggttta aaagcttata
     1081 gaacaaagga tggaaagatt caacttttta gagtagatga aaatgctaag agaatgaata
     1141 aatcatgtga taaactttta atgcctgaaa taccagttga aaaattcata gacgcttgta
     1201 tgcaagttgt caaagctaat gaaagatttg tgcccccata cggtactggt gcaactcttt
     1261 atataagacc tttcatgata ggtgttggtg ataatatagg tgttaaatct gctcctgaat
     1321 ttatattttc agtattctgc cttccagttg gtgcttattt taaaggtgga atgaagcctg
     1381 taaactttat gattgcagat tatgatagag ctgctcctaa aggaactggt gccgctaaag
     1441 ttggtggaaa ttacgcagca agcttaaagg ctcatgaaat agcagcaaaa aaaggatttg
     1501 ctgattgtat atatttagac ccagcaactc acactaaaat tgaggaagtt ggagctgcaa
     1561 acttctttgg aataacaaag aaaggtgaat ttgttactcc atattcagag tcaattttac
     1621 caagtataac aaaatactct ttaatgcaaa tagctaaaga ttatttaaaa atgcctgtat
     1681 cagaaagaga tgttttaata gataacttag atgaattcgc tgaggctggt gcttgtggta
     1741 cagctgctgt aataactcca ataggaggaa tagaatataa gaataaactt catgttttcc
     1801 atagcgaaac cgaagttggt cctattacta aaaaacttta tgatctttta tctggaatgc
     1861 aatttggaga tgtagaagcg cctgaaggat ggatatttga agttaaataa tacataaaaa
     1921 agagagtgag cagtaaactc ctctcttttt ctcttactta ataaaatttt cttttatttc
     1981 tcttagtaat tctttatact tatcaccaga atctattatt ctaaaagttt catcattaac
     2041 tttaataaat tcttcactca atgataagct tatatactgg tctcccatat atatattata
     2101 ctcatttgaa attactggat ttttaaaagg tttaactatt tttatctcct taatatcatc
     2161 atatatattt gctatatcat tttcatcttc tattttataa ctattaaatt ccgaatcatt
     2221 tttcttttct gtaacaacta tatattctgg attttctatt aacatattaa atgcagcctc
     2281 taatttaata ggcataaaga aatatgaaca aacagtaacc aatattgtta taaagaaagt
     2341 aataactaat ttattaagaa gcttcatata tcccctcaaa attaattgct tactttaata
     2401 ctattcccct tacatctaaa ttatgctttt tagctaaatc tatatatatt tctagcatat
     2461 cattttcctc aaaaaacata ggaaaactca aattataatt tccccatata gtcataggct
     2521 tttccataaa atatatggta caaaagaata attccctagt atttaatcta gttataaagg
     2581 acattaaatc tttattatga agctcaaaat aaacactttt tatttcatgt ttattgatat
     2641 ctattaaaat atttttatcc ctttcatcta acccctctaa aagccttgct aaactctcat
     2701 tttctaaatc ttttatgtat aaatctatta ttactttccc cttattaagc tctaatgctt
     2761 cattaaagaa attaataaaa cattcctctt ttattttaaa agaatcatta tccccattat
     2821 attctaaaag cccgtttgaa tatctatcaa atccatctaa tatattatta aatcttattt
     2881 ttattttatt tttaaattta tcttcactta tagattt
//
LOCUS       DAEVGI010000217         2901 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEVGI010000217 DAEVGI010000000
VERSION     DAEVGI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.219"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            231..515
                     /gene="groES"
                     /locus_tag="K8M77_002413"
     CDS             231..515
                     /gene="groES"
                     /locus_tag="K8M77_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011593059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBI7086859.1"
                     /translation="MSIKPLGDRVVIKRLEAEETTKSGIIVTGTAKERPQEAEVVAVG
                     PGAIIDGKRTEMEVKIGDKVLYSKYAGTEVKFEGEEYTILRQDDILAIVE"
     gene            570..2189
                     /gene="groL"
                     /locus_tag="K8M77_002414"
     CDS             570..2189
                     /gene="groL"
                     /locus_tag="K8M77_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483269.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBI7086860.1"
                     /translation="MAKTLLFGEEARRSMQVGVDKLANTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAREIELEDAYENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVTAGANPILIRNGIKTAVEKAVEEIQKISKPVNGKEDIARVAAISAADEKIGKLI
                     ADAMEKVGNEGVITVEESKSMGTELDVVEGMQFDRGYVSAYMVTDTEKMEAVLDNPLI
                     LITDKKISNIQDLLPLLEQIVQAGKKLLIIADDIEGEAMTTLVVNKLRGTFTCVGVKA
                     PGFGDRRKEMLQDIATLTGGVVISDEVGGDLKEVTLDMLGEAESVKVTKESTTIVNGR
                     GNSEEIKNRINQIKLQLEATTSEFDKEKLQERLAKLAGGVAVVKVGAATETELKESKL
                     RIEDALAATKAAVEEGIVPGGGTAYVNVINEVAKLTSDIQDEQVGINIIVRSLEEPMR
                     QIAHNAGLEGSVIIEKVKNSDAGVGFDALRGEYKDMIKAGIVDPTKVTRSALQNAASV
                     ASTFLTTEAAVADIPEKEIPQGAGLGMDGMY"
     gene            2457..2813
                     /locus_tag="K8M77_002415"
                     /pseudo
     CDS             2457..2813
                     /locus_tag="K8M77_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
BASE COUNT         1215 a          287 c          580 g          819 t
ORIGIN      
        1 ataaatagag atatatatga aaaaagtaaa ttgaagaaat tttaaaagaa tagaagaaaa
       61 cttaagataa attaacttaa acatggaaaa taataataat atattccaaa taaataaatt
      121 tagttattga atttacaact tgtaaattat attataatag taagaattag cactctgcat
      181 taatgagtgc taaacaaaaa ataattagta tttttaagga gggtttttta atgagtatta
      241 aaccacttgg agacagggtt gttattaaaa gattagaagc agaagaaact actaaaagcg
      301 gtataatagt tactggaact gctaaggaaa gaccgcaaga agcagaggtt gttgcagtgg
      361 gaccaggagc aataattgat ggaaaaagaa cagagatgga agttaaaata ggagataaag
      421 tattatattc taaatatgca ggaacagaag ttaagttcga aggggaagaa tatactattt
      481 taagacaaga cgatatacta gcaatagttg aatagtttta aaagataagt gatttatata
      541 ttcataatat atttgggagg taaattaata tggctaaaac attattattc ggtgaagaag
      601 caagaagatc tatgcaagta ggagtagata aattagctaa cactgttaag gttacattag
      661 gaccaaaagg aagaaatgtt attttagata aaaaatttgg atcaccatta ataacaaatg
      721 atggtgttac aatagcaaga gaaattgaac ttgaagatgc ttatgaaaat atgggagctc
      781 aacttgtaaa agaagtagct acaaagacta atgatgtggc aggagatgga actactacag
      841 ctaccttatt agctcaagca attataagag aaggattaaa aaatgtaaca gcaggggcaa
      901 atcctatatt aataagaaat ggaattaaga ctgcagttga aaaagctgta gaggaaatac
      961 aaaaaatttc taagcctgta aatggaaaag aagacatagc tagagttgct gcaatttcag
     1021 cagctgatga aaaaattggt aagttaattg cagatgctat ggaaaaggta ggaaatgaag
     1081 gcgttataac tgtagaagaa tctaaatcaa tgggaactga gttagatgtt gttgaaggta
     1141 tgcaatttga tagaggatat gtatcagctt atatggttac tgatactgaa aaaatggaag
     1201 ctgttttaga taatccatta atattaataa cagataagaa aataagcaat atacaagatt
     1261 tattaccatt acttgagcaa atagttcaag caggcaaaaa acttttaata atagctgacg
     1321 atatagaagg cgaagctatg accacattag ttgttaataa attaagagga acatttactt
     1381 gcgttggagt taaagcgcct ggatttggtg atagaagaaa agaaatgtta caagatatag
     1441 ctactctaac aggaggcgtt gttatatctg atgaagtagg cggagactta aaagaagtta
     1501 cattagatat gcttggagaa gctgaaagtg ttaaggtaac taaagaaagt actacaatag
     1561 ttaatggaag aggaaactca gaagagatta aaaatagaat taaccaaata aaattacaat
     1621 tagaagctac tacttctgaa tttgataaag aaaaattaca agaaagatta gctaaattag
     1681 caggtggggt tgcagtagtt aaggttggag ctgccactga aacagagctt aaggaaagta
     1741 agttaagaat agaggatgct ttagcagcta caaaggcagc tgttgaagaa ggaatagttc
     1801 caggtggtgg aacagcttac gtaaatgtaa taaatgaagt tgcaaaatta acttctgaca
     1861 ttcaagatga acaagttggt ataaatataa ttgtaagatc tttagaagaa cctatgagac
     1921 aaatagctca caacgcagga ctagaaggtt cagttataat agagaaagtt aaaaatagtg
     1981 atgcaggtgt aggatttgat gctttaagag gagaatataa agatatgatt aaagccggaa
     2041 tagttgatcc aactaaggtt acaagatcag ctcttcaaaa tgcagcatca gtagcatcaa
     2101 cattcttaac aacagaggct gccgtagcgg atattccaga aaaagaaatt cctcaaggtg
     2161 cagggcttgg aatggatgga atgtattaaa aaataagaaa tattgaatta ctataaaaaa
     2221 taaagatatt aaataagata acttaagact tgaaataaaa taagaaagta gatatataaa
     2281 tgatataaaa gagattcttt taaagaatct ctttttttat gcatataaat gggaaaaatg
     2341 tccataattc taatatgaat ctaaattcaa aagattaggg aggattgtaa atgtttttat
     2401 ttagagtaag taaaatattt aaaaaaagaa atgaaggaaa attaaccaca aaagaagggt
     2461 ttacattaat tgagttaact gttgtcttag cagttatggc aataatttta atggttatag
     2521 ctccaaattt ttcatctgtt aaggatagtg ctaaggcaaa agtagataaa caaaattgtg
     2581 ctgctattga aagatcagtt gaaatgttat tggcggaaga tgctatttca agtagtgtta
     2641 caaatataaa aataacttca agtaatggaa acgtacaagt ttcaggaata agtgataata
     2701 ctagtaaaag taaattggaa gatttacttg aagatttaga taagcctcaa agtggagatt
     2761 cttataatgt agatatagaa aagggaagaa aagttacagt gtctatagta taagtatact
     2821 ttaattaaat caatttttat agggctgtat aaaatataat aataaaaatt atttttatac
     2881 atgtccttat tttcatattt c
//
LOCUS       DAEVGI010000218         2873 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEVGI010000218 DAEVGI010000000
VERSION     DAEVGI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.220"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            126..1748
                     /gene="yjeM"
                     /locus_tag="K8M77_002416"
     CDS             126..1748
                     /gene="yjeM"
                     /locus_tag="K8M77_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBI7086861.1"
                     /translation="MESKEKSKLTLTALILMIFTSVYGFTNMPRTFYLMGYSGIIWYI
                     MSAILFFIPYAFMMAEYGAAFRKEKGGIYSWMAKSVNPKFAFIVTFMWFSSNLIWMVS
                     VSSSIWIPLSNVFFGKDTTSTWNIFGLTGPRAMSVLAIILLIIITFISSKGLNKISKI
                     TSIGGTVVALSNVVLILGGFFVLASNGFKTAQGFNMEQFIHSPNPAYQSPLGVLGFVV
                     FAIFAYGGIEVVGGLVDQTENPKKNFPKGIKISAFVIVIGYAIGILCIGFFVNWNRDL
                     TGSDVNMANIAYIIVNYLGYYIGEAIGLSTSACTMLGNFFARFIGLSMFLALIGAFFA
                     LSYSPLKQLIEGTPKDIWPEKWTKLNKENMPATAMWIQCLTVIIFVLCCSIGGDDSSK
                     FFNYLILMGNVAMTLPYMFLSFAFSFFKKKKEIVKPFEVYKNYKSALVWSIIVTLTVG
                     FANVFTIIQPLTSEKKDYVAVIFQISGPIIFGLLAWLIYRRYENNVLKKQISNIDNKI
                     DTELKKIEKAEEEVTSETIGVIDIEVHDDDSK"
     gene            complement(1848..2807)
                     /locus_tag="K8M77_002417"
     CDS             complement(1848..2807)
                     /locus_tag="K8M77_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086862.1"
                     /translation="MKYKRYFISLVLILAMVSIAEITGEREIIFPEIAALVVGAWILE
                     KQPWKVSKLGLVCLMTLCSIVGVLIVRYVNLGLTIEVLIGLVFTGLILKLSKTTLVPI
                     ISACILPIVLRTETWVYPISVFILTILIVSIKYFIEDYGVSEEVKEEFKEDIAKLERN
                     TSLRKKEFIRLFKILITVGILTYFSVKFNITLLIAPPLIVAFIELTNEHCKFRQRSKS
                     LLFLFIVVAILGFIFRIGFNEYLGLPLWLCTIFLLISLFICLEIFNMYFPPVAAIAVL
                     PTLLTSKQVMFYPFQIAIGCFIFITVAIIFFREEKCIVDTFKN"
BASE COUNT         1049 a          382 c          397 g         1045 t
ORIGIN      
        1 tttcttttta tttacaaatc attaatttat taacatactt tgctttaatt taatgtatat
       61 tatttgtata cttaagaaaa tgaggcttaa ataaagcttt aaaaatatat gagaggtgat
      121 tttatatgga atcaaaagaa aaaagcaaat taaccctaac agcattaatt cttatgatat
      181 ttacatctgt ttatggcttt actaatatgc caagaacctt ttatttaatg ggttatagtg
      241 gaataatatg gtatataatg tcagctattt tattttttat tccttatgct tttatgatgg
      301 ctgaatatgg cgctgccttt agaaaagaaa aaggtggtat atattcttgg atggctaaat
      361 cagtaaatcc taaatttgct tttatagtaa catttatgtg gttttcttca aatctaatat
      421 ggatggtatc tgtatcatct tctatatgga ttcctttatc taatgtattt tttggtaaag
      481 atactacttc tacttggaat atattcggtt taacaggtcc tcgtgcaatg tctgtattag
      541 ctataatatt attaataata ataacattca tatcttctaa aggattaaat aaaatttcaa
      601 aaattacatc cataggagga acagttgttg ccttgtctaa tgtagtttta attcttggtg
      661 gttttttcgt actagcaagc aatggtttta aaactgctca aggctttaac atggaacagt
      721 ttatacattc tccaaatccc gcatatcaat cccctttagg cgtacttggc ttcgttgtat
      781 ttgcaatatt tgcttatggt gggatagaag tagttggtgg attagttgac caaactgaaa
      841 accctaaaaa gaattttcct aaaggaataa aaatttctgc ctttgtaatt gttattggtt
      901 atgcaatagg aattttatgt attggtttct ttgttaattg gaatagggat ttaactggtt
      961 ctgatgtaaa tatggcaaat atagcatata ttatagtaaa ttatcttggt tattacatag
     1021 gagaagctat tggcttaagt actagtgctt gtacaatgtt aggtaatttc tttgctagat
     1081 ttataggact ttcaatgttt ttagcactta taggtgcatt cttcgcttta agttactctc
     1141 ctttaaagca attaatagaa ggtactccta aggatatttg gcctgaaaaa tggacaaaat
     1201 taaacaagga aaatatgcca gctacagcta tgtggattca atgtctaaca gtaattatat
     1261 ttgttttatg ttgctcaatt ggtggagatg attcatctaa attctttaac tacttaatac
     1321 ttatgggaaa tgtagccatg acattacctt atatgttttt atcctttgct ttttcattct
     1381 tcaaaaagaa aaaagaaatt gtaaaacctt ttgaagtata taaaaattat aaaagtgctt
     1441 tagtttggtc tataatagta acattaacag taggttttgc taatgtattt acaataatac
     1501 aacctcttac ttcagagaaa aaagattatg ttgctgtaat attccaaatt tctggtccta
     1561 tcatatttgg tttattagca tggttgatat atagaagata tgaaaacaat gtgctaaaaa
     1621 aacaaatttc aaatattgat aacaaaattg atacagaact taaaaaaata gaaaaagctg
     1681 aagaagaagt tacaagtgaa acaataggag taatagatat agaagttcat gatgatgatt
     1741 ctaaataagc ttgaaggact atactttaaa gttgactata ataataatcc ctaaaaaaat
     1801 aattggtcat ataaattatg accaattatt tttttaataa atttatttta attcttaaaa
     1861 gtgtcaacaa tacatttttc ttctctaaaa aaaataatag caacagttat aaatataaag
     1921 catcctatag caatttgaaa tggataaaac attacttgtt tactagttaa tagcgttggt
     1981 aaaacagcaa ttgctgcaac tggtgggaag tacatattaa atatttcaag acatataaat
     2041 aaactaatta ataggaaaat agtacaaagc cataaaggca gtcctaaata ttcgttaaat
     2101 ccaattctaa atataaatcc taaaatagct acaacaataa ataagaataa taaacttttt
     2161 gaacgttgcc taaatttaca atgttcattt gtaagttcta taaaagcaac tattaaaggt
     2221 ggtgctatta ataaagtaat attaaattta acagaaaaat atgttaaaat acccacagta
     2281 ataagtatct taaataatct aataaattct ttttttctaa gtgaagtatt tctttcaagt
     2341 ttagcaatat cttctttaaa ttcttctttt acctcttcac ttactccata atcttctata
     2401 aaatacttaa ttgaaactat aagtatagta agtataaata cggatattgg ataaacccaa
     2461 gtctcagttc taagtacaat aggcaaaata caagctgaaa taattggcac aagagttgtt
     2521 tttgatagct ttaatataag cccagtaaaa actaatccta ttaaaacttc aatagttaac
     2581 cctaagttaa catatcttac aataagtacc cctactatag aacatagtgt cataagacat
     2641 actaatccta atttactgac cttccaaggt tgcttttcta aaatccaagc tccaacaact
     2701 aaagcagcta tctctggaaa tattatctct ctttcccctg taatttctgc aatacttacc
     2761 atagctaaaa taagtacaag tgatataaaa tatcttttat atttcatatt atgacatcct
     2821 ttgtttaaaa atttcagatt aattttatca catcaaaagt attttgttaa aaa
//
LOCUS       DAEVGI010000219         2824 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEVGI010000219 DAEVGI010000000
VERSION     DAEVGI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.221"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            193..2793
                     /gene="clpB"
                     /locus_tag="K8M77_002418"
     CDS             193..2793
                     /gene="clpB"
                     /locus_tag="K8M77_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBI7086863.1"
                     /translation="MNVDKMTLRVQQSLNDAYEIAVKYNNQQLDIIHLFSALVNQKDG
                     LIPNIFEKMGVNIDSLKRDIHIQIDRMPKVLGEAAQSSGVTATRRINEVLIKAEEISK
                     QFEDSYISVEHVMLAMIDIDKNGFVGEILRKNRITKDGFLKILNEVRGSQRVDSQDPE
                     GTYEALEKYGTNLIELVKQHKLDPVIGRDEEIRRAIRILSRKTKNNPILIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKVIISLDMGALIAGAKYRGEFEERLKAVLKEVQNSEG
                     KIILFIDEIHTIVGAGKTDGAMDAGNLIKPMLARGELHCIGATTFDEYRQYIEKDKAL
                     ERRFQPVIVEEPTVEETVSILRGLKERFEIHHGIRIHDSAIVAAAKLSHRYIQDRYLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDIIRRKILMLETEKEALSKENDDASKERLAALE
                     KELAELQDKNDEMTIKYEKEKSHISAIRDLKAELDEARGLVEKYEREYDLNKVAELKY
                     GKIPELERKIKEQEESMEKDNENALLKEEVTENEISEIISKWTGIPVAKLVESEREKL
                     LRLEEELRERVIGQDEATTAVANAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIVRIDMSEYMEKHAVSRLIGPPPGYVGYEEGGQLTEAVRRNPYSVIL
                     FDEIEKAHDDVFNLFLQILDDGRLTDNKGKTVDFKNTIIIMTSNIGSGYLLENKSSEG
                     IEDDIRENVMNEMKLRFKPEFLNRVDDIIMFRPLSSEGIKKIIDIFLRDVENRLRERN
                     ITLEVTDKAKEILAEEGYDPVYGARPLKRYISNVLETEIAKKIIAGEIYDGSVALIDG
                     VDGKIIVSRK"
BASE COUNT         1234 a          277 c          536 g          777 t
ORIGIN      
        1 taatttgtta agaaaaactc tttaaaatta aaatttaatt ataaaatcga ttttataata
       61 ataaagtatt ataaaataac actataaatt aataaattct aaaaaattaa aatagacttt
      121 gactttcttt gacttttgta gtattattat tacataagat acaaaaaaaa gataggatat
      181 tgaggtgata aaatgaatgt agataaaatg acattaagag tacaacaaag tttgaatgat
      241 gcttatgaaa tagcagttaa atataataat caacaattag atataattca tttattttca
      301 gcactagtga atcaaaaaga tggtttaatt cctaacatat ttgaaaaaat gggtgttaat
      361 atagattcat taaaaagaga cattcatatt caaatagata ggatgcctaa ggttttagga
      421 gaagcggcac aatcatcagg agttactgct acaagaagaa taaatgaagt gttaataaag
      481 gcagaagaaa tttcaaaaca atttgaagat tcatatataa gtgttgagca tgtaatgctt
      541 gcaatgatag acatagataa aaatggattt gtaggagaaa tcttaagaaa aaatagaata
      601 actaaagatg gattcctaaa gattttaaat gaagttagag gaagtcaaag agtagattca
      661 caagatccag aaggaactta tgaagcttta gagaaatacg gtactaattt aatagaactt
      721 gtaaaacaac ataaattaga tcctgttata ggaagagatg aagaaataag aagagccatt
      781 agaattcttt caagaaaaac taaaaataat ccaatactaa taggagaacc tggagttggt
      841 aaaactgcta tagttgaagg attagcagaa aggattgtaa gaggagatgt gccagaagga
      901 ctaaaggata aggtaattat ctcattagat atgggggcat taatagctgg tgctaaatat
      961 agaggagaat ttgaagaaag attaaaagct gttttaaagg aagttcaaaa ttcagaagga
     1021 aaaataattt tattcataga tgaaattcat acaattgtag gtgctggtaa aacagatggt
     1081 gcaatggatg caggaaactt aattaaacca atgcttgcaa gaggagaact tcactgcata
     1141 ggagctacaa cgtttgatga atatagacaa tatatagaaa aagataaagc actagagaga
     1201 agattccaac ctgtaatagt tgaagaacca acagttgaag aaacagtatc aattttaaga
     1261 ggattaaaag aaagatttga aattcaccat ggaattagaa tccatgattc agcaatagta
     1321 gctgctgcga aactttctca tagatatatt caagatagat atttaccaga taaggctata
     1381 gatttaatag atgaagctgg tgctatgata agaagtgaaa tagattctct tccaactgaa
     1441 ttagatatta taagaagaaa gatcttaatg cttgaaactg aaaaagaagc tttatcaaaa
     1501 gaaaatgatg atgcatctaa agaaagatta gctgctctag aaaaagagct tgcagagctt
     1561 caagataaaa atgatgagat gacaattaaa tatgaaaaag aaaaatctca tatttcagca
     1621 attagagact taaaggctga gttagatgaa gcaagaggat tagttgaaaa atatgaaaga
     1681 gaatatgatt taaataaagt tgcagaactt aagtatggaa aaattccaga acttgaacgt
     1741 aaaataaaag aacaagaaga aagtatggaa aaagacaatg aaaatgcttt attaaaagaa
     1801 gaagttactg aaaatgaaat ttcagaaata atctcaaaat ggactggaat tcctgttgca
     1861 aaactagttg aaagtgaaag agaaaaactt ttaagactag aagaagagtt aagagaaaga
     1921 gtaataggtc aagatgaagc tacaactgca gtggctaatg ctgtaataag agcaagagca
     1981 ggacttaaag atgaaagaaa acctatagga tcatttatat tcctaggacc tactggagtt
     2041 ggtaaaactg agcttgcaaa aactttagct agaaatttat tcgatagcga agacaacata
     2101 gtaagaatag atatgtctga atatatggaa aaacatgcag tatctagatt aataggacct
     2161 cctccaggat atgtaggata tgaagaaggg ggacaattaa cagaggctgt tagaagaaac
     2221 ccatattcag taatattatt tgatgaaata gaaaaagcac atgatgatgt atttaactta
     2281 ttccttcaaa tattagatga tggtagatta acagataaca aaggtaaaac tgttgatttt
     2341 aaaaatacta taataattat gacttctaat ataggtagtg gatacttatt agaaaataaa
     2401 tctagtgaag gtatagaaga tgatattaga gaaaatgtta tgaatgaaat gaagttaaga
     2461 ttcaaaccag aatttttaaa cagagtagat gatatcataa tgtttagacc tttaagcagt
     2521 gaaggaatta agaaaataat agatatcttc ttaagagatg ttgaaaatag attaagagaa
     2581 agaaatataa ctcttgaagt tactgacaaa gctaaggaaa tattagctga agaaggatat
     2641 gatccagtat atggagctag accattaaag agatatataa gcaatgtctt agaaactgaa
     2701 atagctaaga agataattgc aggagaaatt tatgatggat ctgttgcttt aatagatgga
     2761 gtagatggaa aaattatagt ttctagaaaa taaaatataa taaatttata aggaccatag
     2821 atta
//
LOCUS       DAEVGI010000220         2708 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEVGI010000220 DAEVGI010000000
VERSION     DAEVGI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.222"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            560..1078
                     /locus_tag="K8M77_002419"
     CDS             560..1078
                     /locus_tag="K8M77_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI7086864.1"
                     /translation="MPKIIKNVKEDLLTEGKKQLLSNGYLNLNIRNITKKCNIGTGTF
                     YNYFKNKEQLVIDILSNDWTSILNISNEIIKEDISFKEKLIFISNNINYFLKNYRLIF
                     SEMAKHTSKNHYVIEPMYETLEKLIEIHIEKGEINPPISSYKFAYFLLNNLILTSRSE
                     LTIDDLLLLLNI"
     gene            complement(1148..2593)
                     /locus_tag="K8M77_002420"
     CDS             complement(1148..2593)
                     /locus_tag="K8M77_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBI7086865.1"
                     /translation="MSKKGSKVLGFLQRIGKSLMVPIAVMPALGLLLRLGDKDLLNIP
                     WISAAGGAAFGDNMAMLFAVGIGFGLSDENNGVGGLAGLLGFLVMKNVATSFDPSINM
                     GAFGGVVAGVVGGLLYNKFKDIKVPQFLGFFGGKRFVPIITSMVCLILGVFFGYTWPT
                     FQAGLDGFANIMVAAGAIGAGIYGILNRLLIPIGLHHVMNTVIWFQLGSFTDPVSGQI
                     ATGDIARFLAGDPTAGIYTAGFYPIMMFGLPAACLAMYVCAKKKNKAVVGGMFLSLAL
                     TAIITGITEPIEFAFMFLSPILYVIHAILTGISLAVAYALNVHLAFSFSGGLIDYILY
                     FGKGQNQLIILLMGIVAFVIYYFLFMFFIKKFNLKTPGREDDFDDENEDVENNSKTVS
                     KSEDNPSKGGTLAEKAEVVLEALGGKENIEVLDNCITRLRLTLKDASKIDEVTLKKAG
                     ASGIMKLDGKNVQVIMGTLADPLASQMKKLL"
BASE COUNT          971 a          450 c          286 g         1001 t
ORIGIN      
        1 tgttaatatc ttctgtttaa ttttcaaaga ccatttcgtc gtcgccctca agcgacttcc
       61 ttagtttatc atcacttcga aactttgtca acaacttttt ttcaaaactt ttaatttgtt
      121 ttgttgagtt gtttgtgtgt ctctcagcga cgagatttat cttaccacgt tacataactt
      181 attctaatta catagatgtt tattttacat aattattgtt aatattcttt atttttcatt
      241 aaaatactat ataattctta tattgataca cctggtaaag tttgaattga aatttccctt
      301 aatattccta taaatactct tattctaaga ataagattta ctatattatt cttagaataa
      361 ataaaaaaac tcttttttta tttaaattta tctaaatata tacaccaata taataattta
      421 tagatattac ttagataaaa ttatacttat attttctcta tatttaattc aatcttttct
      481 ttaaaacact ttaaatttga atttaataat cttaaatact ataatttata tatagttctt
      541 taatgaagga ggccttctta tgcctaaaat tataaaaaat gtaaaagaag atttattaac
      601 tgaaggaaaa aaacaattgc tgtctaatgg atatttaaat ttaaatataa gaaatataac
      661 taagaaatgt aatataggta ctggaacctt ctataactat tttaaaaaca aggaacaact
      721 tgttattgat atactatcta atgattggac ttctatttta aatatatcta atgaaataat
      781 aaaagaagat atttcattta aagaaaaact aatttttatt tctaataata taaattattt
      841 cttaaaaaac tatagactta tattttcaga gatggctaag catacctcta aaaatcacta
      901 tgttatagag ccaatgtatg aaactttaga aaagctaatt gaaattcata tagaaaaggg
      961 agaaatcaat ccccctatat cctcatataa gtttgcttat tttctactaa ataatcttat
     1021 attaacatct cgttctgaat taactataga tgacctatta ctattattaa atatataaaa
     1081 taaaaagcca taaaccatta aatggtttat ggcttttatt ttataagtat aattttttct
     1141 ttaattatta aagtaatttt ttcatttggc tagctaaagg atctgctaaa gttcccataa
     1201 ttacttgaac attctttcca tctaatttca ttattccact agctccagcc ttttttaaag
     1261 taacttcatc tattttagaa gcatctttta aagttaatct taatcttgtt atacaattgt
     1321 ctagaacttc aatattttct tttcctccaa gagcttctaa aacaacttct gccttttcag
     1381 ctaaagtacc acccttagat ggattatctt ctgattttga tactgtcttt gaattatttt
     1441 ctacgtcttc attttcatca tcaaaatcat cttctctacc aggagtttta agattaaact
     1501 tcttaataaa gaacataaat aagaaataat aaattacaaa tgcaacaatt cccattaata
     1561 atatgattaa ttgattttga ccttttccaa agtataaaat atagtcaata agtccacctg
     1621 agaaactaaa tgctagatgt acattaagag catatgccac tgctaaggat atacctgtta
     1681 aaattgcatg tataacataa agtattggtg ataagaacat aaaagcaaat tctattggtt
     1741 ctgtaatacc tgttattata gctgttaatg ctaatgataa gaacattccg ccaaccactg
     1801 ccttgttttt ctttttagca caaacataca ttgctaaaca tgcagctggt aaaccaaaca
     1861 tcattattgg atagaaacct gctgtataaa tcccagctgt tggatctcca gctaaaaatc
     1921 ttgcaatatc tccagttgca atttgacctg aaactggatc tgtaaagctt cctaattgga
     1981 accaaattac tgtgttcatt acatggtgta atccaattgg tattaataat ctatttaata
     2041 ttccatatat tccagcccca atagcacctg ctgctaccat tatattagca aatccgtcta
     2101 atccagcttg gaatgttggc caagtatatc caaagaatac ccctaaaata agacatacca
     2161 tcgatgttat tattggaaca aatctttttc caccaaagaa tcctaaaaat tgaggaactt
     2221 taatatcttt aaatttatta tataaaagtc ctccaacaac tccggctaca actccaccaa
     2281 aagctcccat atttatactt ggatcaaatg aagttgcaac atttttcata actaaaaatc
     2341 ctaaaagacc tgctaatcct ccaactccat tattttcatc tgaaagtcca aatcctatac
     2401 ctacagcaaa aagcattgcc atattatctc caaaggcagc tccaccagca gcgctgatcc
     2461 aagggatatt taataagtct ttatctccaa gtcttaataa taatcctaaa gctggcatta
     2521 ctgctatagg aaccattaaa gatttaccta ttctttgaag aaagcctaaa acttttgaac
     2581 cttttttact catttgttca ttcctcctta taagtttttt tcatttagtt taagtttgtg
     2641 taatacttta aaaaaattaa tttcttcaaa gcattagata ttctaaaaat aaaatataaa
     2701 agttatct
//
LOCUS       DAEVGI010000221         2678 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEVGI010000221 DAEVGI010000000
VERSION     DAEVGI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.223"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(133..411)
                     /locus_tag="K8M77_002421"
     CDS             complement(133..411)
                     /locus_tag="K8M77_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086866.1"
                     /translation="MKYVKAQDVLPEEIIEILQKYVDGKYLYIPRKNENQKAWGENSG
                     IRKSLNARNTEIFKKYKDGTSINTLAKEYFLSEKSIRRIISDKKKLCS"
     gene            1025..1357
                     /locus_tag="K8M77_002422"
     CDS             1025..1357
                     /locus_tag="K8M77_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086867.1"
                     /translation="MYKVENDTYECPVEALSNILGRKWVAEIIWGIKDNKVRFGELQR
                     LLKGCSKKMLSQQLELLMENKIIINDKKTINNIIESTYYLSKSGILLLPILEKMISWG
                     NINLSCEE"
     gene            1383..2594
                     /locus_tag="K8M77_002423"
     CDS             1383..2594
                     /locus_tag="K8M77_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBI7086868.1"
                     /translation="MENINFFIVFIEGILSIFSPCILPILPIYLSMLSNSSVEDIRDS
                     NFKSGVLIRNTLFFTLGISTTFFILGSSISALSSFFNTNKNIIMILGGVIILFMGLFY
                     LGVINLNILNREKRLNFKYKNMSPVSAFVLGFTFSFGWTPCIGPILASVLVMASSSKN
                     LLMSNLLILVYTIGFILPFIIASLFYGKLFKKFDGIKKHMDLIKKISGIFIIIAGLIM
                     LVGGIRNMNNEIKINNNLKINKSESVSKDSKNESNNKNQEEGNKIPPIDFTLYDQYGN
                     KHTLSEYKGKTIFLNFWATWCPPCRGEMPYIDELYKEYNENKDDVVILGVASPNLGRE
                     GSEEHIKNFLKEENHVFPVVLDENGSMVYQYGINAFPSTFIINKEGYITKYIPGAMTK
                     ETMKTLIESER"
BASE COUNT         1003 a          272 c          411 g          992 t
ORIGIN      
        1 tgtaatgaat tcataatcat attatttaat agcttgtttt aaatttagat gttattactt
       61 taagtactgc tttaatgaga taataacatc ttttaaaata agtattcaat ctaataatta
      121 attctgtaat gattatgagc atagtttctt tttgtcactt ataattcttc ttatactttt
      181 ctcagaaaga aagtactctt ttgcaagagt atttatggag gttccgtctt tatatttttt
      241 gaatatctca gtatttctag cattaaggct ttttcttatt ccactatttt caccccaagc
      301 cttttgattt tcattctttc ttggaatata aagatatttt ccatccacat atttttgtaa
      361 tatttcaatg atttcctctg gtaacacatc ttgtgctttt acatatttca tttccttgct
      421 cctttaattt acgtaatatt attgaagcaa agaatactaa aagctatgtg tcacagaacc
      481 taatttatat tcagctaact caatacatta ggctccaaac aaagcatagc ttactcttag
      541 taatctttgc aaggagcaag atgatttata actgaatgta ttatttacct tcaagaaaaa
      601 cacctccttt atttaatgtg tattatatca cattaaattt aaatggtaac taaaaagtgc
      661 ctaattgtta aaaaattata tgttgataga attaggatag agattaaaga ggtttttgtt
      721 ttagatataa aataaggatt taaatctttg atagtctttt atgttaataa ggtatttttt
      781 acctgtttag tcaaaactac tttgaaatat ggtatataat tcacatttaa taaagatatt
      841 gatttaatta ggaatatggc tttgttttag aaaaagttaa tttattaagt ttaactaata
      901 gctaatacaa ggccataagt aatatgtgga cttatttgaa ataaattcat aatatttaca
      961 tatactttta gtaattttag gttaaatttt aattatgttt tataaggaat tgaggaggat
     1021 ggaaatgtat aaagtagaga atgatactta tgagtgtcca gttgaagcct tatcaaatat
     1081 tttaggaaga aaatgggttg ctgagataat atggggaatt aaagataata aggttaggtt
     1141 tggagaatta caaagattat taaagggatg tagcaaaaag atgttaagtc aacaattaga
     1201 attattaatg gaaaataaaa taataattaa tgataaaaaa actataaata atataattga
     1261 atcaacttat tatttaagta aatctgggat attgttatta cctattttag aaaagatgat
     1321 atcttgggga aacataaatt tatcctgtga ggaataagta gaaataaagg gggaaaggtt
     1381 tagtggaaaa tattaatttc tttattgttt ttatagaggg aatcttatca attttctcgc
     1441 cgtgtatatt gccaatacta ccaatatact taagtatgtt atctaatagt agtgtagagg
     1501 acataaggga ttctaatttt aaaagtggtg ttttaataag aaatacatta ttttttactt
     1561 taggaatatc aactacattt tttatattag gttcatcaat aagtgctttg agctcattct
     1621 ttaatactaa taagaatatt ataatgattt taggtggagt tattatatta tttatgggct
     1681 tattttattt aggtgtaata aatttaaata tattaaatag agaaaaaaga ttgaatttta
     1741 aatataaaaa tatgtcacca gtatcagcct ttgtgttagg atttacattt agttttggat
     1801 ggacaccatg tatagggcct atattagcat cagttcttgt aatggcatca agttcaaaaa
     1861 atctattaat gtctaactta ttaatattag tttatacaat tggttttata ttaccattta
     1921 taatagcatc attattctat ggtaaattat ttaaaaagtt cgatggaata aagaagcata
     1981 tggacttaat taagaaaata agtggaattt ttatcataat agctggttta ataatgcttg
     2041 taggtggaat aagaaatatg aataatgaga taaagattaa taataacctt aagataaata
     2101 agagcgaaag tgtaagtaaa gatagtaaaa atgaaagtaa caataagaat caagaagagg
     2161 gaaataaaat tcctccaata gattttactt tatatgatca gtatgggaat aaacataccc
     2221 ttagtgaata taaaggaaaa actatattct taaatttctg ggcaacttgg tgccctccat
     2281 gtagaggaga aatgccttat atagatgagc tttacaaaga atacaatgaa aataaagatg
     2341 atgttgtaat ccttggagta gcttcaccta atttaggaag agaaggttca gaagagcata
     2401 taaagaattt cttgaaagaa gaaaatcatg tattcccagt tgtacttgat gaaaatggat
     2461 ctatggttta tcaatatgga ataaatgctt ttccatcaac tttcattata aataaggaag
     2521 gatatataac taaatatatt ccaggtgcaa tgacaaagga aaccatgaaa actttaattg
     2581 aaagtgaaag ataacaatgg gattattaca gtattattaa gtttcaagtt tactttattg
     2641 aacttagaat cttatttgga tatcttaatt aatttata
//
LOCUS       DAEVGI010000222         2672 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEVGI010000222 DAEVGI010000000
VERSION     DAEVGI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.224"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(64..138)
                     /locus_tag="K8M77_002424"
     tRNA            complement(64..138)
                     /locus_tag="K8M77_002424"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Gln,seq:ttg)
     gene            complement(142..218)
                     /locus_tag="K8M77_002425"
     tRNA            complement(142..218)
                     /locus_tag="K8M77_002425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Arg,seq:tct)
     gene            complement(344..1036)
                     /locus_tag="K8M77_002426"
     CDS             complement(344..1036)
                     /locus_tag="K8M77_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086869.1"
                     /translation="MHNLFHRRSKIEENPEKFWRELITKNETLKGRMFKDEPITEDTK
                     YLHYVIFNRKVGFQNVWVMVPNFKRLIEFIEYVFMPEAYYKWVEGKKKLITHIPSIDV
                     EKIISMINRKATEEEKEKMKNDISALRKLKGLSADNGMRKLKIFCSRFNNNWLGNDDE
                     FLYLKAFGSAEELGKFVVETNLQTDCEDCYEKTIGMTTEEWFKVCKNAHKNKEDEQKF
                     KKVLFKHLEDIV"
     gene            1226..1630
                     /locus_tag="K8M77_002427"
     CDS             1226..1630
                     /locus_tag="K8M77_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI7086870.1"
                     /translation="MSYYCEICGDYADVHHIVHRSEGGLDFKLNYKYLCKKHHRSKIG
                     PHRDKVVDLNYKLEMQNNLRELFIKSFYTGNDIKIMLGLTPGSLKKLTKNLKLYKEGY
                     SSDEIIFNLMGRKVYSEEYIEDFILQKAISCI"
     gene            complement(1823..2371)
                     /locus_tag="K8M77_002428"
                     /pseudo
     CDS             complement(1823..2371)
                     /locus_tag="K8M77_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
BASE COUNT          890 a          422 c          266 g         1094 t
ORIGIN      
        1 tagtatgttt atatattaac atactgttaa tatgttgtca agtttttaaa ccaagttaaa
       61 aactggctgg gatagcagga tttgaacctg cgaatgacgg agtcaaagtc cgttgcctta
      121 ccgcttggcg atatcccagt atggtgtacc ttaagggatt cgaacccctg acccacggct
      181 tagaaggccg ttgctctatc cagctgagct aaagatacaa acttgaatca acaaaaatta
      241 ttatacatag ttatttaaaa aatgtcaaca tttttttcaa tttattttat atttttatta
      301 atactaagcg atttcaattt taaaatatca agagaattta attttataca atatcctcca
      361 agtgtttaaa taaaactttt ttaaattttt gctcatcttc tttattttta tgagcatttt
      421 tacaaacttt aaaccattct tctgtagtca ttcctatagt tttttcataa caatcttcac
      481 aatctgtttg taagttagtt tctactacaa actttcctag ttcttcagca cttccaaaag
      541 cttttaagta taaaaactca tcatcatttc caagccaatt gttattgaat ctactgcaga
      601 atatttttag ttttctcatt ccattatcag cacttaatcc ttttaatttt cttaatgctg
      661 aaatgtcatt tttcattttt tctttttctt cttcagttgc ttttctattt atcatagaaa
      721 ttatcttctc tacatcaatt gaaggtatat gagttattaa tttctttttc ccttcaaccc
      781 atttatagta tgcttctggc ataaatacat actctataaa ttctattaat ctcttaaagt
      841 taggtaccat aacccaaaca ttttgaaatc caacttttct attgaatatt acataatgta
      901 ggtatttcgt atcttcagtt ataggttcat ccttaaacat tcttcctttt agggtttcat
      961 tttttgttat taattccctc caaaatttct cgggattttc ttctatcttt gatcttctat
     1021 gaaataagtt atgcatctca aaaccccctc atatccttta tattttaatt atattgctaa
     1081 ctttttttaa cagcaaagtg taaaaaatac taaattcctt tgtatacctt aatctttatc
     1141 tgaaaagctc cgttatattg attaaactgt ttttttatgc tattttatta ctataaatgg
     1201 gtattaaaat gttaaggagg attatatgtc ttattattgt gaaatttgtg gtgattatgc
     1261 agatgttcat cacatagttc ataggtcaga aggcggttta gattttaaac ttaattacaa
     1321 gtatttgtgt aaaaaacatc acagatctaa aataggcccc catagagata aagtcgtaga
     1381 tttaaattat aaacttgaaa tgcaaaacaa cctaagagaa ctctttataa aaagttttta
     1441 tactggtaat gacataaaaa ttatgttagg tcttacccct ggttctttaa aaaaattaac
     1501 taagaaccta aaactttata aagaaggata ttctagtgat gaaataatat ttaatcttat
     1561 gggcagaaaa gtttattcag aagaatatat agaagatttc atattgcaaa aagcaataag
     1621 ttgtatatag aaagctgtat caaagctact tcagttttga tgcggcttta tttttttatc
     1681 aattatttaa tattcattat gaaataaagc tattatttct cttttaaaca actttaactt
     1741 ttatatcctc attttgtatc tcaactaatt tctttataat tacttatgct ttaaagaact
     1801 aatttttaat atcctaagat ctttatattg taaaaccaca tattaaacta ttataatcat
     1861 gtattcttaa atccttaaaa atcttcttag caatcgctaa atgtccttta tgatttgctc
     1921 ttgcttctac taaccatgat tctataagct ctttttgaac tttgcttaac ctatcccaat
     1981 actcataatg atctattaag aatatttctt cttccttaga aacctctctt ataactttta
     2041 catgcccttc tatatctatt ccatttttta ttggactttg taatataggt attgagttta
     2101 tatttttaat cttgcttact atattaacta aattatgttt aaattcctct ggagtaattt
     2161 tttcatttcc tccaatcata ataaaaacag ctttaggatt atattttgat actaaatctt
     2221 cgaagttatt cattaaatat tctgaattaa cttttttatt accaaaatta atcataaatt
     2281 tttccctttt atttatgctt ccatcattta attcccacct aattctctct tccacatatt
     2341 caacataatt tttataccca aaggtatctc cttcaatcat tataataccc tctttcttat
     2401 ttccttctaa ctttatttta tatacctttt tacctaaaaa attaatattt attaaaaact
     2461 aaatttttat aaaatactta aatattttat aggttactat ttaattaatc ctatgccctt
     2521 atatgtttat taattaaaga atattaatat gtttagaatt taagagttat aaaaaaatac
     2581 ttctttattt aattttaata aaaaaaagac ttcaataaaa ttatgaagtc ttccaaaaaa
     2641 atctctaaaa acaaaaaagc atcgattata aa
//
LOCUS       DAEVGI010000223         2664 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEVGI010000223 DAEVGI010000000
VERSION     DAEVGI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.225"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            112..1467
                     /locus_tag="K8M77_002429"
     CDS             112..1467
                     /locus_tag="K8M77_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 family protein"
                     /protein_id="HBI7086871.1"
                     /translation="MRKKDILELKKRLKKDHCTFTKICGCYVNGEKNIILNFRETFLN
                     LDEDEYFKYLEIAKKVMSGTIGNNILELNFPLNDYLVNERQVSLMQLKNSKLKDDELL
                     NDFYKLVIDNYDYTGNFLILLFHDAYDVITKTKDNLKVDESEEVYEYILCAVCPVSLS
                     GPGLRYFEEENQIKARIRDWVVEAPANGFVFPAFIDRSSDVNSVMYYTKNAKDAHPEL
                     MENVLGCTSKQTATIQKQTFKSIIKDSFLDEEKAEKVFMEVQENLNNMIEEHNSMYED
                     TPDCEPISLTDKDVQNILIESGIPEDITSQIEKSYVESFGEDLPLAENLIDNKTLKAN
                     EQRKKEEHLEKQVAILKTRLEEVKKEAAMDKEEDSITENDDLVSDETLESNSEEILES
                     NLEESEDNTTINSEYDIVLQVKPEKIPEIKSQIIDGQKCIVIPINDDEQTTVNGLDGL
                     I"
     gene            complement(1669..2622)
                     /locus_tag="K8M77_002430"
     CDS             complement(1669..2622)
                     /locus_tag="K8M77_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086872.1"
                     /translation="MKGKKLSVSGVILIISFLIYIVYLVSSNILLNYGMEFRESVKNF
                     ILLDKFVFIAFGNIIFIKIIWKEKGIGGFAKTFTTFLSFIISFVLIFGFILIPYVFES
                     RVEALAYKEGRLVVAASSDGLHNSRISFYEPVNSIVMKNSDIEDLHHNAPNNCYYYEK
                     SNFLENPDFDIREESNFEDLKPWSAKKKFSHEVFNYLGNKTYDFGCIVIKFDKKETWV
                     KEASIERNIVKYDGVNIISENIDTIKENISKAEKNHDDSTIYEEFLDEKGKQNIRIGR
                     KYRFNTKDEYSIYYYDTTLVLDDNGIVTSVKYLNRNFEDID"
BASE COUNT         1050 a          370 c          323 g          921 t
ORIGIN      
        1 gcctttggag agccataaaa atcgtagtag cttaggcaag gcgaggttct atgaagaagg
       61 aattttctca atatggatat atttgaccat attttgagta gcagtttata gatgagaaag
      121 aaagatatac ttgaattaaa aaaacgtcta aaaaaagacc attgcacctt tactaaaata
      181 tgtggatgct atgtcaatgg agaaaaaaat attattttaa actttagaga aacttttctt
      241 aacttagatg aagatgaata ctttaagtat ttagaaattg ctaaaaaagt aatgtctgga
      301 actattggaa acaatatttt agaacttaac tttccactaa atgattatct tgttaatgag
      361 agacaagttt ctcttatgca attaaaaaat agtaaattaa aggatgatga acttcttaat
      421 gatttttata aattagtcat agataattat gattacacag gtaacttttt aatacttctt
      481 ttccatgatg cttatgatgt tataactaaa actaaggata accttaaggt agatgaatct
      541 gaagaagtat atgaatatat tttatgtgca gtatgtccag tttctctttc tggtcctggt
      601 cttagatatt ttgaagaaga aaatcaaata aaagcacgta ttagagattg ggtagttgaa
      661 gcccctgcca atggatttgt attccctgct tttatagatc gtagctcaga tgtgaattct
      721 gttatgtatt atactaaaaa tgctaaggat gctcatccag aattaatgga aaatgtatta
      781 ggttgtactt caaaacaaac tgctactatt caaaagcaaa cattcaaatc aattattaag
      841 gattctttct tagatgaaga aaaagcagaa aaagtattta tggaagtaca agaaaattta
      901 aataatatga tagaagaaca taactctatg tacgaagata ctcctgactg tgaacctatt
      961 tctttaacag ataaagatgt tcaaaatata ttaatagaaa gtggaattcc tgaagatatt
     1021 actagccaga ttgaaaaatc ttatgtggaa agctttggag aagatcttcc tttagctgaa
     1081 aacttaattg ataacaaaac tctaaaagca aatgaacaga gaaaaaaaga agaacatctt
     1141 gaaaagcaag tggcaatact aaaaactaga cttgaagaag tgaaaaaaga agctgccatg
     1201 gacaaggaag aagattctat tacagaaaat gatgatttag tttctgatga aactttagaa
     1261 agtaactcag aagaaatctt agaaagtaat ttagaagaat ctgaagataa tactactatt
     1321 aatagtgaat atgatattgt acttcaagta aagcctgaaa aaatacctga aataaaatct
     1381 caaataattg atggtcaaaa atgcatagtt attcctatta atgatgatga acaaactacc
     1441 gttaatggat tagatggttt aatttaaatt agttgaaaat aaaataatac aaatgaggtt
     1501 aaatcaatta aatatttgat ttaaatttaa ataaatccac cttaaaacta aggtaaaata
     1561 aaaattttta ttaaatcaaa acttactaaa aaacctcatt tgtatttatg tttttatata
     1621 aattatattt taataaatta atcaggtttt atttactaag tataaacctt aatctatatc
     1681 ttcaaaattc ctatttaaat atttaactga agtaactatt ccattatcat caagaactag
     1741 agttgtatca taataatata tgctatactc atctttagta ttaaatctat attttctacc
     1801 aattctaata ttttgcttac ctttttcatc taaaaattct tcatatatag tactgtcatc
     1861 atgatttttc tccgctttac ttatattttc tttaatagta tcaatatttt cacttattat
     1921 atttacacca tcatacttaa ctatatttct ttctatagaa gcttctttca cccaggtttc
     1981 ttttttatca aatttaatta ctatacaccc aaaatcataa gttttgtttc ctaaataatt
     2041 aaacacttca tggctaaatt ttttctttgc actccatggc tttaaatcct caaaattaga
     2101 ttcttccctt atatcaaaat caggattttc taaaaagttg gatttttcat aataataaca
     2161 attgttagga gcattatgat gtaaatcttc aatatcacta tttttcataa caatagaatt
     2221 tacaggttca taaaaactta tccttgaatt atgtaatcca tcactagaag cagctactac
     2281 taatcttcct tctttatatg ctaaagcttc cactcttgac tcaaaaacat aaggtattaa
     2341 aataaaacca aatattaaaa caaaacttat tataaaagat aaaaatgtag taaacgtctt
     2401 agcaaatccc cctattcctt tctctttcca aattatttta ataaatatta tattaccaaa
     2461 agctataaat acaaatttat ctaaaagtat aaaattctta acactttctc taaactccat
     2521 accatagttt aataaaatat tgcttgaaac caaataaaca atataaatta aaaaacttat
     2581 tattaaaata actcctgaaa cacttaattt cttccccttc atgtaactcc tccccctaaa
     2641 aatataaata acttaaatag ctta
//
LOCUS       DAEVGI010000224         2663 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEVGI010000224 DAEVGI010000000
VERSION     DAEVGI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.226"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1653
                     /locus_tag="K8M77_002431"
     CDS             <1..1653
                     /locus_tag="K8M77_002431"
                     /inference="COORDINATES: protein motif:HMM:NF019844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7086873.1"
                     /translation="CMNPDGVIVRGTNNGPGRCAVTTRVDMNRCFPYNFSPQYTSRNY
                     TGPNPLGSPEAVALKNLVERINSSSSEMVVLDFHGWMNFTQDNAELGRYFGNQFGFGH
                     NNVYSSGFFSSWASTLRNTKATLIEYPTNTYSYNDVINKNYIGKTFNGIINILKNNSN
                     SGDVDTGGNDNSNETFYDAKGEITNVSTTLNVRSGPGTSYTAIGTLRGGDRVSIIAKV
                     KPSSEQLYWYRIKFGSGSGYIRSDFVKLIESGVNEIPMDRVGIIKVSSTLNVRSGPGT
                     IYDIVGTLNNGASVRVVAKVRPLTEEFYWYKIEFGNGIGYVRSDFVSFEHIPNPDVPG
                     NNELVKAKDRAEAIIKKMFGTENRLVLNQFTTIIDGVVKVEVLYGLKYYHNPDAIIKL
                     DIENGEFKSSNPIQQTLIDFIYFLIGKNQYINVYIKELLAEVENGSLGVKINPVTLEM
                     SFVIETKIFEDKEASIKNMFFIEVKFKLNCKELENLFEESFKSVVREIVDFIFNITNI
                     AVQAICIAGLSLIIIYTPFDELGIALVTFLNMLKNSPLPANV"
     gene            1876..2663
                     /locus_tag="K8M77_002432"
                     /pseudo
     CDS             1876..2663
                     /locus_tag="K8M77_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1044 a          240 c          468 g          911 t
ORIGIN      
        1 tgtatgaatc ctgatggagt tatagttaga ggaactaata atggtccagg taggtgtgct
       61 gtaacaacta gagttgatat gaatagatgt ttcccatata acttttcacc tcaatatact
      121 tcaagaaact atacaggacc taatccatta ggatcaccag aggcagttgc acttaaaaat
      181 ttagttgaaa gaataaattc tagttctagt gaaatggtag ttttagattt ccatggatgg
      241 atgaatttca ctcaagataa tgcagaatta ggaagatatt ttggaaatca atttggattt
      301 ggacataata atgtatatag ttcaggattc ttctcaagtt gggcatcaac attaagaaat
      361 acaaaagcta ctttaataga gtatcctact aatacttata gttataatga tgttataaat
      421 aaaaactata taggaaaaac tttcaacgga ataataaata ttcttaaaaa taattctaat
      481 agtggagatg ttgatactgg aggaaatgat aattcaaatg aaacttttta tgatgcaaaa
      541 ggagaaataa caaatgtatc aacaacttta aatgtaagga gtggtcctgg aacatcgtac
      601 actgcaatag gaacattacg tggtggggat agagtatcta ttattgcaaa agtaaagcca
      661 tcatcagaac aactttattg gtacagaata aaatttggaa gtggaagtgg atatattaga
      721 agtgattttg taaaacttat tgagtctggg gtcaatgaaa tacctatgga tagagtaggt
      781 ataataaagg tttcatcaac attaaatgta agaagtggtc ctggaacaat atatgatata
      841 gttggaacac ttaataatgg tgctagtgtt agagtagtag caaaagtaag accattaaca
      901 gaagaatttt attggtataa aatagagttt ggaaatggaa ttggttatgt tcgtagtgat
      961 tttgttagtt ttgagcatat tcctaaccca gacgttcctg gcaataatga attagtaaaa
     1021 gctaaagata gagcagaagc aattattaaa aaaatgtttg gcactgaaaa tagattagtt
     1081 ttaaatcaat ttacaacaat aattgatgga gtagttaagg ttgaagtttt atatggcttg
     1141 aaatattatc ataatccaga tgcaataata aaattagata tagagaatgg agaatttaag
     1201 tcaagtaatc caatacaaca aactttaata gactttatct actttttaat tggaaaaaat
     1261 caatatataa atgtttatat aaaggagtta ttggctgaag tagaaaatgg ctcattaggt
     1321 gtaaaaataa atccagttac tttagaaatg tcatttgtaa tagaaacaaa aatatttgaa
     1381 gataaagaag cttctattaa aaatatgttc tttattgaag tgaaatttaa attaaattgt
     1441 aaggaattag aaaatctgtt tgaagaatca tttaaaagtg tagtaagaga aattgttgat
     1501 tttatattca atataaccaa tatagccgtt caagcaattt gtatagccgg attatcttta
     1561 ataattatat atacaccttt cgatgagtta ggaattgcat tagtgacgtt tctaaatatg
     1621 cttaagaatt ctccattacc agctaatgta tagattttct tttaattaaa tattattttg
     1681 cttttaagat agtaattttt atacaaaatt actatctatt tttttagtta aataattaac
     1741 atataattaa aacactataa tttattaaaa tgatattata tatgatataa gggagaggat
     1801 ttaatgaaga aatcatattt agttattata ttagcattaa taattacatt aataggagtg
     1861 ataggattta ataaaaataa gttttcaagt aaatttgatc aacttttaga agttagtaaa
     1921 ggtaaagata gtgaaaatga attaagtgga ggatttccat ataatataga atttttagat
     1981 tataatataa ctataaattc agtgagatta tttgaggatt atgcattatt tgattattca
     2041 gtaaaaaaaa ttgatggagg atatataaac attacagatg ttaatattac tggaaatgtg
     2101 aattttaaag ggagagaatt ttatatatct ccaaatgatg ttgaaagaag atgggaatat
     2161 tctagtgtac gtattatttc aactggagaa ataattgcca aagaaaataa ggattcacct
     2221 gccaatttaa gcttttcaat taatgctagg tcagctgatg gtagttatat agcaaagtct
     2281 gtatctataa aagaagaagt ttatttaaca gatgaagatt atttaagttt aaataaaaaa
     2341 attacttttg ataatgttga ttttgaaatt cgtagtttcg cgaattttga gtttggttca
     2401 ttactgtata tttttgttgg taataaggat gaagaagaat gtaaaagaat tgcaaatcag
     2461 tatgttataa agttaataag tggagattat gaaagaagct ttgatttaga aaaatgggtt
     2521 ggatttactg atttagctat aagaaaatat agtagcattg atagtagata tgataaaaat
     2581 tcacaacacg atcaatttta tatgtcaaga atgttttata atttaaatga agttgataga
     2641 gaaaatattg aagtttactt ggt
//
LOCUS       DAEVGI010000225         2651 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEVGI010000225 DAEVGI010000000
VERSION     DAEVGI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.227"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(498..>2651)
                     /locus_tag="K8M77_002433"
     CDS             complement(498..>2651)
                     /locus_tag="K8M77_002433"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012471.1,HMM:NF013627.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7086874.1"
                     /translation="GSLSPYEAATPLYKKNGMTHVVYDTSKGPNTKSGYVKFEGLPEL
                     PDTSIQIPSVSHSQATVEVYGLSGRSRQLKVYKIGSGSKVLFAGFALHGWEDKWDNDG
                     LALVNIANSLISKIGDYKAQNGLHGWTVYIAPCMNPDGVVVRGTHNGPGRCAVKSRVD
                     MNRCFPRGFSAQYNSRNYTGPEALGAQEAVALKNFIEKINKTADELVVIDAHGWLGCT
                     YGSSSIAQYFTNQFGFSHKGESFGGYFSYWAQGLPRTRGLLLEYPNTNSYSEVLSRNY
                     IGKTFNAIINILKNNPNGGDGDTNTDSNYDKWVNKDGKWYYYENGKMVTGWKLVDGEW
                     YYLGSDGVMVTGWIELGGIWYYLSDSGAMVKGWQTISGKTYYFNDSGHMLTGKQVIDG
                     KNYEFNESGHLISGDGGISSSEVTYIAVGKVINVQSFLNVRKGPGTNYDSIGQVYQGD
                     KVSIVAKNGTWYKIEYGSGYGYVHSNFINIVLDEDINKKILKFAGNTGFFENMNLEFE
                     FFNKRIPLGAISFSPLITVEAEISLNMKLDNGSKINLTLTPDELTAEFLGKFNALGVT
                     SSIDKRHLTSVFEKLTLSRNVIDGFRYQVILGPAGTINVTFESTFEYDFGNGNKESIY
                     QRLYFTIHPHLNPNLPSVVRIADIDIETDETVKIEDKNIMQLIHKGIVQFVSYVHYQF
                     SKITIPDIKPITPQQVSIIGSVALGVIIILACVLV"
BASE COUNT          873 a          498 c          273 g         1007 t
ORIGIN      
        1 tcaataatta ttttaatatt tcttataatg gtacttaaat caattcaata ttactatttt
       61 ctcaaagata aacaaaatgt atatgtttta attaaaaaaa tatatatgag attgggaata
      121 atcctaattt tattgactat attaaattta ctctctattt ttacccaaaa caaaatccaa
      181 cctgtattca tctcactttt attaattgaa atttttgctg aaaacttatt aacagtattg
      241 gatttaatta aaaagaattt tttagtttta aatttagaac aaaaagtttt aagaaaaaaa
      301 atttttctat ataagatacc ttctatgtat tttttattaa tacttataga atatacattt
      361 ataatatata tatcacctaa cataatcacg ataaccaaca tcatactttt tattttggtt
      421 ttatatttct taattactaa caaaaagcaa aagcccaata gataacccta ttgagctttt
      481 catttatttc tgatcaacta tactaaaaca catgctaaaa taattattac ccctaacgca
      541 acagagccaa taatagatac ttgctgagga gttattggtt taatgtcagg tattgttatt
      601 tttgaaaatt gatagtgtac ataagataca aattgaacta ttcccttatg aattaattgc
      661 attatatttt tatcctcaat tttaacagtc tcatctgttt ctatatcaat atctgctatt
      721 ctaacaacag aaggaagatt tggatttaaa tgtggatgaa ttgtaaaata taacctttgg
      781 taaatagatt ccttattccc atttccaaaa tcatactcaa aggtagattc aaaagttaca
      841 ttaatagttc cagctggacc taatataact tgatatctaa acccatcaat tacatttcta
      901 cttaaagtta acttttcgaa cacactagtt aagtgtcttt tatctattga tgatgttact
      961 cctaaagcat taaattttcc taaaaactcc gctgttaatt catcaggtgt caaggtaaga
     1021 tttatctttg aaccattgtc taatttcata ttaagagaaa tttccgcttc aacagttatt
     1081 aatggactaa acgatattgc tcccaaagga attctcttgt taaaaaattc aaattctaaa
     1141 ttcatatttt caaaaaaacc tgtattacca gcgaacttta atattttttt atttatatct
     1201 tcatctaaaa caatgttaat aaaattagag tgtacatatc cataacctga accatattct
     1261 attttatacc atgttccatt tttagctact atacttacct tatctccttg atatacctgt
     1321 cctattgagt cataatttgt tcctggtcct tttcttacat tcagaaatga ttgtacattt
     1381 attacttttc caacagctat atacgttact tcagatgatg atataccacc atccccacta
     1441 atcaagtgtc ctgattcatt aaattcataa tttttaccat ctataacttg tttgcctgtt
     1501 aacatatgtc ctgaatcatt aaagtaatat gtttttcctg atattgtttg ccagccctta
     1561 accattgctc cactatcact taagtaatac catatacctc ccaattctat ccatccagtc
     1621 accataactc catcactacc taagtaatac cattcaccat ctacaagctt ccatccagtt
     1681 accatttttc cattttcata ataataccat ttaccatctt tatttaccca tttatcataa
     1741 tttgaatctg tattggtatc tccatcacca ccattaggat tattctttag aatatttatt
     1801 atagcattaa atgttttacc tatataattt ctacttaaaa cctcactata actattagta
     1861 ttaggatatt ctaataaaag acctcttgtt cttggtaatc cttgtgccca atatgaaaaa
     1921 tatcctccaa aagattctcc tttatgtgaa aatccaaatt ggttagtaaa atattgagca
     1981 attgaactac ttccatatgt gcatccaagc catccatgtg cgtctataac tactaactca
     2041 tcagctgttt tatttatctt ttctataaaa ttctttaatg caactgcttc ttgtgcccct
     2101 aaagcttctg gacctgtgta gtttcttgaa ttatattgag ctgaaaaacc tcttgggaaa
     2161 catctattca tatcaactct agattttacc gcacatctac ccggtccatt atgtgttcct
     2221 ctaactacaa ctccatcagg attcatacaa ggtgctatat atactgtcca accatgaaga
     2281 ccattttgtg ctttatagtc tcctatctta ctaattaaag agttcgctat atttactaaa
     2341 gctaatccat cattatccca tttatcttcc catccatgta atgcaaatcc agcaaataat
     2401 accttactac cacttcctat tttataaact ttcaattgtc tacttcttcc actaagtccg
     2461 tatacttcaa ctgttgcttg agaatgtgat actgaaggta tttgtatact agtatcaggt
     2521 aactctggta atccctcaaa ttttacataa cctgattttg tatttggtcc tttagaagta
     2581 tcatagacta catgagtcat tccatttttc ttatacagtg gtgttgcagc ttcatatgga
     2641 cttaatgatc c
//
LOCUS       DAEVGI010000226         2643 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEVGI010000226 DAEVGI010000000
VERSION     DAEVGI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.228"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..624)
                     /locus_tag="K8M77_002434"
                     /pseudo
     CDS             complement(1..624)
                     /locus_tag="K8M77_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            1427..1852
                     /locus_tag="K8M77_002435"
     CDS             1427..1852
                     /locus_tag="K8M77_002435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086875.1"
                     /translation="MIKKLISGALLATTITVFSSITALAAGASAPFSASFYSGEEAVS
                     YLDGSQNGKYYSLTPGRASLDVTSMYGKGAVNVPIRLKRFWSSDMVVDTKEVEWPGHY
                     TFGGVPETSSKYYLFFQCNPSISNTKTQYKITGTVNDWH"
     gene            2000..2386
                     /locus_tag="K8M77_002436"
     CDS             2000..2386
                     /locus_tag="K8M77_002436"
                     /inference="COORDINATES: protein motif:HMM:NF016763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBI7086876.1"
                     /translation="MFYLICLISITLLPITIYYIKAPYIITPNINFVPFYGILDYTSS
                     KILLFNTLGNLILFVPFMIFIEILKDGKVSLLKGLFLILICSLLIEGIQYIEMYFQLA
                     ERIVDINDVILNTLGGGIGYYIYIII"
BASE COUNT          946 a          391 c          284 g         1022 t
ORIGIN      
        1 ctttaaagca ttttttactg ttgatttata tccagtatat gttgcataga aatatccacc
       61 ataaacaatt accattatta aggcagttac taatgatgaa acccctatat ctggtctatt
      121 aaacatagtt aggaatccat ttacaacctt tattgctact actgagtgaa ctaaggctac
      181 aactaaaggt aatccaaaat aaatactaac ctgtgtaaat attgatttat ttatcatctt
      241 ttgagataca ccaattcttc ttaaggattt atatctatca atactatcgc tagcctctga
      301 taattgttga agagcaagga cagcagtact tgatattaag aatataactc ctaaatatat
      361 tcctataaac aatactatat ttgtcattcc attattatct tcataaatac tttctcttgt
      421 atatccattt acaaaaccat atttacttga atcaacaact ccatctctat attcattgaa
      481 taatttttgt actctttctt cctcatttga atttttaggg aagtttatat taattttatt
      541 tgaagtaggc tttaaattag aaacaatatt atctggcact attaaagtga aaaagttatt
      601 tgtcattcca gtactttcaa atgccgttgc caaaaatcta taaatatcta taaatcttgt
      661 ttaattccct tttctacgag tcagattttg aaaactataa atagtaatct actttccttt
      721 gatataactc tccgagggcg taaattcgga tacaacgcat cctacatata caagttacct
      781 tgtttgtgat atttcttgta ttactatatt tgcttggttt tcgcttctaa gtacgcactt
      841 agattttctt taaacactaa aaccctcaag gttctaccct atgactatat cactttcgca
      901 atatatggga ttcacaatag ctaacaatat tttatcctaa acacaaacat tttaactata
      961 ctaagatctt ataaatatta tttttataga tttataacaa cagtcacctc cctccttgtt
     1021 tcatcttaca atcttatttt atcctcttta atagaaatca catatttatt tatctttcat
     1081 acaccttaca attttgtaag ttttctatat tataataaat catttacttt atctcttctc
     1141 tctctatcat ctatatgatc tttatgaatt tcatcaatag ttttttcttt catttaaatc
     1201 ttcatatata tttatattat cttaaaatgg atttccactc ataaatccat ctttaaatcc
     1261 tctaatagca tcatctagtt caaatattaa aaataaaatt acacaaataa ctaatataat
     1321 tctgaccagt tatgcacaaa aagagcccag ttattcacta attatttact taatattatt
     1381 attttattaa aattacattg taataaacgt aaggggatat ttaacaatga taaaaaaatt
     1441 aatatctggt gctttattag caaccacaat aacagtattt tcatcaataa cagctttagc
     1501 tgcaggagct tctgctccgt ttagtgctag cttttatagc ggagaagaag cagtttctta
     1561 tttagacgga agtcaaaacg gtaaatatta ttcactaact cctggaagag caagcttaga
     1621 tgttacaagt atgtatggaa aaggtgctgt aaatgtacca ataagattaa aaagattttg
     1681 gtcctcagat atggttgtag acactaaaga agtagaatgg ccaggtcatt atacatttgg
     1741 tggtgtacca gaaacttcat caaagtatta tctattcttt caatgtaatc cttcaatttc
     1801 taatacaaaa actcaatata aaattacagg aacagtaaat gactggcatt aatatatgat
     1861 ggcagcagtt tatacaccaa gattattaat tttatatgga acaattttat tactttttag
     1921 tatagttata agaatttctt tttataaatt aaaaagaaaa aaaattaata taaaaaaaga
     1981 tttattggta aatttatttg tgttttactt aatttgttta attagtataa cactgctacc
     2041 tataaccatt tattatataa aagcacctta tataataact cctaatatta attttgtacc
     2101 attttacgga atacttgatt atacttcatc aaaaatttta ctttttaata ctttaggtaa
     2161 tttaattctt tttgttcctt ttatgatttt catagaaata ttaaaagatg gaaaagtttc
     2221 tttgttaaaa ggattatttt taattttaat atgttcactt ttaatagaag gtatacaata
     2281 catagaaatg tattttcaac ttgctgaaag aatagtagat atcaatgatg ttatattaaa
     2341 tacacttggt ggtggaattg gatattatat atatatcatt atttaaaaac ttcactatcc
     2401 ataaaaattc aaaaacttaa gttatttaaa ctaaaaatga gagatagtta ttgtaatata
     2461 gctatctctc ttcttaattg tctaatagtt aaagtaatat atacttgtaa tattatagac
     2521 ttaatctcta cattatcaat aaggtttata tttttcaaca taaaatatta ttttaaagct
     2581 cttaaccttc gcttctatat tataaaaatt ccttagaaga aaatatttaa taaataatgt
     2641 taa
//
LOCUS       DAEVGI010000227         2626 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEVGI010000227 DAEVGI010000000
VERSION     DAEVGI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.229"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            108..977
                     /locus_tag="K8M77_002437"
     CDS             108..977
                     /locus_tag="K8M77_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBI7086877.1"
                     /translation="MESRYCEANKVTIISILWNIVLAIIKILAGIIGKSNAMIADGAH
                     SASDIISSVGVLIGNKIASIPKDKGHNYGHEKAETLVSFLLAILLVILSIKIGYNGVY
                     SLFHLNEVQIPTAFPLVVSVISIIIKEYQYRITIKVAKKTNSPALRADAWHHRSDALS
                     SVAAFIGIGGAILGFKSLDSIASIFVALFIAKVGIEILIGSANELMDISIDEEKEKEI
                     KTIVDEVEGIKSLREIRTRKYGAMAYVDLVICVDKDISVFEGHRIASDLENLILKKMK
                     FIKGVNIHVEPLI"
     gene            1197..2570
                     /locus_tag="K8M77_002438"
     CDS             1197..2570
                     /locus_tag="K8M77_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI7086878.1"
                     /translation="MNKYEYIIIGFGKGGKTLAGYLGKLGKKVAIIEKSDKMYGGTCI
                     NVGCIPTKTLVNKSKVSLYKGLNTFEEKAREYRKSIEEKNALIEALRDKNYNMLNNNE
                     NVDVFNGTASFISNTEILINSEKEDIILEGEKIFINTGATTIIPNIQGIKSSSKIYNS
                     TTIMELKELPKHLVIVGGGYIGLEFASIYASFGSKVTVIEAFDRIAGREDEDISKSIK
                     EILEKKGIEFLLGSKVKSFEEIHGEVEVSYENSLGELNKITGDAVLIAIGRKPNTEEL
                     NLEAAGVKVTERGAIEVNNKLKTNIPNIWAIGDVNGGPQFTYISLDDFRIIKDNLFGE
                     GKRSTDDRKFIPYSVFIEPNLSRVGLSEKEALEKGFEIKTAKLDVNTIPRAKVIGETE
                     GIMKAIVDVKTNKILGCTLLCAESAEIINIVTLAMKADEDYTFLRDNIFTHPTMSEAL
                     NDLFSLI"
BASE COUNT         1058 a          239 c          467 g          862 t
ORIGIN      
        1 ttataataat taattatcaa ttgataatat aaattatata tttaccttga ttttatagat
       61 tttatttaag ttttaatata ttaattgata atattagggg gataaatatg gagtcaagat
      121 attgtgaagc taataaagta acaattatat ctatattatg gaatatagtt ttagctataa
      181 tcaaaatatt agcaggtata ataggaaagt ctaatgctat gatagcagat ggtgcacatt
      241 cagcatcaga tattataagc tctgtaggag tattaattgg gaataaaata gcaagcatac
      301 caaaggataa gggacataat tatggacatg aaaaagctga aactttagta tcttttttat
      361 tagctatcct actagtaatt ttatctataa agattgggta taatggtgtt tattcacttt
      421 ttcatttaaa tgaagtccaa attccaactg cctttccttt agttgtatca gttatatcta
      481 ttattataaa ggaatatcag tatagaatta ctattaaggt agctaagaag acaaattcac
      541 cagcacttag ggctgatgca tggcaccata ggtcagatgc attatcctcg gttgcagctt
      601 ttataggaat aggtggagct attttagggt ttaaatcact agattccata gcatcaatat
      661 ttgttgctct ttttatagca aaggtaggaa ttgaaatttt aataggttca gcaaatgagc
      721 ttatggatat ttctattgat gaggagaagg aaaaggaaat aaaaactata gtagatgaag
      781 ttgaaggaat taagtcttta agagagatta gaacaagaaa gtatggggct atggcttatg
      841 tagatctagt aatatgtgtt gataaagata tttcggtttt tgaggggcat aggattgcta
      901 gtgatttaga gaatctaata ttaaaaaaaa tgaaatttat taaaggtgtt aatattcatg
      961 ttgaaccttt aatataactt taagatttat atttataaag taattttaat aaggatagat
     1021 aaactattct tattttttta tatttattta atatgtggta actaaaaagt gcctaattgt
     1081 ttaaattaat acaagttgct aaaattaaat taacttaaaa gagaataatg tattttaaaa
     1141 gagattttag gatgaattta tataaaaatt aatattaaaa gtttggagtg aaaagaatga
     1201 ataaatatga atatattata ataggatttg gaaaaggtgg gaaaacactt gctggttact
     1261 taggaaaact aggtaaaaag gttgctataa tagaaaaatc agataagatg tatggtggaa
     1321 catgtataaa tgttggatgt attcctacaa aaactttagt aaataaatca aaagtatcct
     1381 tatataaagg gttaaatacc tttgaagaaa aagcaagaga gtatagaaaa tctatagaag
     1441 aaaaaaatgc tttaatagaa gctttaaggg ataaaaatta taatatgcta aataataatg
     1501 aaaatgtaga tgtatttaac ggaacagctt catttatttc aaatacagaa atacttatta
     1561 atagtgaaaa agaagatata attttagagg gagaaaaaat cttcataaat acaggagcta
     1621 ctactataat tcctaatata caaggaataa aaagtagcag taaaatatat aacagtacta
     1681 ccataatgga attaaaagaa ttaccaaaac atttagttat agttggtggt ggatatattg
     1741 gattagaatt tgcatcaatt tatgcaagtt ttggctcaaa agtaacagta atagaggcct
     1801 ttgatagaat tgctggcaga gaagatgagg atatttctaa aagtataaaa gaaattctag
     1861 agaaaaaagg aattgaattc ttattaggat caaaagttaa atcttttgaa gaaatacatg
     1921 gagaagttga agtttcttat gaaaatagct taggagagtt aaataaaatt acaggagatg
     1981 cagttttaat agctataggt agaaaaccaa atactgaaga attaaattta gaggctgcag
     2041 gtgttaaagt aacagagagg ggagctatag aagttaataa taaactaaaa acaaatatcc
     2101 ctaatatctg ggctattgga gatgttaatg gtggtcctca atttacatat atatctttag
     2161 atgattttag aattataaaa gacaatttat ttggagaagg aaagagaagt actgatgata
     2221 gaaaatttat tccatactca gtatttattg aaccaaactt atctagagtt ggattaagtg
     2281 aaaaagaagc tttagaaaaa ggctttgaaa taaaaactgc taaattagat gttaatacta
     2341 ttccaagggc aaaggttatt ggagaaactg aaggaattat gaaagctata gtagatgtaa
     2401 agacaaacaa gatattaggt tgcacacttt tatgtgcaga atcagctgaa ataataaata
     2461 tagttacctt agccatgaaa gctgatgagg attacacttt cttaagagat aatatattta
     2521 ctcatccaac tatgagtgag gctttaaatg atttattctc attaatttag ttaaaaatta
     2581 caattttata ttattatatt aaaaagggcg tgtatcaaaa taactt
//
LOCUS       DAEVGI010000228         2612 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.230, whole genome shotgun sequence.
ACCESSION   DAEVGI010000228 DAEVGI010000000
VERSION     DAEVGI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.230"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            281..820
                     /locus_tag="K8M77_002439"
     CDS             281..820
                     /locus_tag="K8M77_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBI7086879.1"
                     /translation="MKFKYCPLCGEKLELKNSWDEGMIPYCSKHDMMFFDLPKPCIVV
                     AVIKGDEIILLKQSYIYENSKVLISGYVGVDECAEETVYREVKEETGITVKDIKYLGS
                     DFVQGKELLMLTYLAYYESGEIEKSTEVEGAAWYNIEDALCELNEDSIGKRVVKKVLK
                     EIGYEGTNAFRCEGSNCNV"
     gene            1230..2525
                     /gene="lysA"
                     /locus_tag="K8M77_002440"
     CDS             1230..2525
                     /gene="lysA"
                     /locus_tag="K8M77_002440"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBI7086880.1"
                     /translation="MKLFGSMKVEGNELYVGGVKASDLSKEYGTPLYVIDEELVRGNC
                     RRYYNAMKCEERGNRVTYAGKAFINMSMCNLVNEENLYLDVVSGGELYTAYKAGFPLE
                     RIYFHGNNKSDYEIDLGVRLGIGRFIVDNIHELEVLNSIAQEYGRVQKVYLRITPGVE
                     AHTHEYIKTGQLDSKFGFPVIGDTVYDAIKRAMELEYIELNGLHCHIGSQIFDLEPFE
                     DTTEIMLNLINDIKETLGYEIKELDLGGGFGIYYTEGDKPKEIEEYCSVIINKADEIC
                     RKLNMNVPILSIEPGRSIVGNAGLTLYTVGAIKEIPNIRKYVSVDGGMSDNIRPALYS
                     ANYESIIANRVFDNSKEIVTVAGKCCESGDVLLNSIEMPRMETGDILAIISTGAYGHS
                     MANNYNRIPKAAVVSVSNGISKVMCKRETYEDLLRNECF"
BASE COUNT         1011 a          214 c          531 g          856 t
ORIGIN      
        1 tttaaagtta gaatttatta tttaaagtta ggatttagta tttaaagttg aatttataga
       61 aaagatataa taagtatttg agaatatgaa aggtggtgaa ctttagttag cttaatttat
      121 atattactat tcttatctaa aatagctaat ttttataagt tatagatggt gattttaaga
      181 aattacagaa gaaaatatat ttgctttaac ttagaattag ataaatttga aataaaaaat
      241 tatagaaggt aatatatttg ctttactaaa gaaattaatt atgaaattta aatattgtcc
      301 attatgtggt gaaaaattag agttaaaaaa tagctgggat gagggtatga taccttattg
      361 cagtaagcat gatatgatgt tttttgattt acctaagcct tgtattgttg ttgctgtaat
      421 aaaaggtgat gagataattc ttttaaagca aagttatata tatgaaaatt ctaaagtcct
      481 tatatctgga tatgtaggtg tagatgaatg tgcagaggaa actgtttata gagaggtaaa
      541 ggaagaaaca ggaataacag ttaaagatat aaaatattta ggtagtgatt ttgtacaagg
      601 aaaagagtta cttatgctaa cttatttagc atattatgaa tcaggagaga tagagaaatc
      661 aactgaggta gagggagctg cttggtacaa tatagaggat gctctttgtg aattaaatga
      721 ggatagcata ggaaaaagag tggtaaaaaa agttttaaaa gaaataggtt atgaaggtac
      781 taatgcattt agatgtgaag gaagtaattg taatgtataa atttttttat taataatttt
      841 agtttatact attgcattat ttgagatttt ttagtaacat tataaacaat aattaatata
      901 ataaaagcat aaataatttg aaaataggac caaagtagag gtgccgtaat taagagtagt
      961 cataagtagc tgacaagtgt tttatgtatg atgaaaggaa ttatggccga agagatatta
     1021 atggtgatta atatttctgg gtatatgtat aatatgcata taactgtcac tttgaaaaaa
     1081 gtggagtgct acaaggtacg ctaagttgcg attaaggcag caaaggtgtt cctttgctgt
     1141 ttttatttat tggaattaaa tgccagaaaa gtcggtagct ttgcatactt ttatactctg
     1201 ccactaaaaa atttgtggag ggaaaagata tgaaattatt tggatcaatg aaagtagaag
     1261 gaaatgagtt atatgttgga ggagtaaaag caagtgattt aagcaaggaa tatggaactc
     1321 ctttatatgt tattgatgaa gagttagtga gaggcaattg tagaagatac tataatgcta
     1381 tgaagtgtga agaaagagga aacagagtta cctatgcagg aaaggcattt ataaatatgt
     1441 ccatgtgcaa tttagttaat gaagaaaatt tatatttaga tgttgtttca ggtggagagc
     1501 tttatactgc ttataaagca ggtttcccat tagaaagaat atatttccat ggtaacaata
     1561 aatctgatta tgaaatagat ttaggagtaa gattagggat tggaagattt atagttgata
     1621 atattcatga actagaagtt cttaattcta tagctcaaga atatggaaga gttcaaaaag
     1681 tttatttgag aataactcct ggagtagagg cccataccca tgaatatata aaaacaggtc
     1741 aattggactc aaaatttgga ttccctgtaa ttggtgatac agtttatgac gctattaaaa
     1801 gagcaatgga attagagtat atagaactta atggattgca ttgtcatata ggttctcaaa
     1861 tatttgactt agaacctttt gaagatacaa cagaaattat gctaaatctt ataaatgata
     1921 taaaagaaac cttaggatat gaaataaaag aattagactt aggtggagga tttggtatat
     1981 attatactga aggggataag ccaaaagaaa tagaagaata ttgttcagtt ataataaata
     2041 aagctgatga aatatgcaga aagttaaata tgaatgttcc tatattaagt atagagccag
     2101 gaagaagtat tgtggggaat gcaggattaa ctctttatac tgtaggagct ataaaagaaa
     2161 ttcctaatat aagaaaatat gtaagtgtag atgggggaat gagtgataat ataagaccag
     2221 ccttatacag tgcaaattat gagtcaataa tagctaacag agtttttgat aattctaagg
     2281 agatagttac tgtggctgga aaatgctgtg aatcagggga tgtgctttta aattctatag
     2341 aaatgcctag aatggaaaca ggagatatat tagctataat atctacaggg gcatatggac
     2401 attcaatggc taataattat aatagaattc ctaaggcggc agttgtgagt gtaagtaatg
     2461 gaatttcaaa agttatgtgt aaaagagaga cttatgaaga tttgcttaga aacgagtgtt
     2521 tttaattaat atataaagtt gtatggtaaa aaaactatac aacttttttt attaaaagta
     2581 tttagaataa tgtattaaag tatataataa aa
//
LOCUS       DAEVGI010000229         2595 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEVGI010000229 DAEVGI010000000
VERSION     DAEVGI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.231"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            180..965
                     /locus_tag="K8M77_002441"
     CDS             180..965
                     /locus_tag="K8M77_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBI7086881.1"
                     /translation="MNKKNILVLVGSLICVALLGYFTYSTVSTLMKSKSVSQEASVTK
                     DTSSENKDKNSQDDLSERLKETNDKKEENKNNTDEKKSEKTANSSENKSANDKSNNST
                     NENKKNTSDKNTNSKETLNNDKNKSENSKSESKNTISYSSYLKDLETEKYTLKNDEKL
                     SDVAKKFKDTCTINSSLNIIKSLNQISNTSSLESGDTVLVPVNAFQDGKKYSVKEGDT
                     WYSIARKHYPKYNHEVVINFLMDINPFKNDILPLGEDIFLPNI"
     gene            complement(1034..1288)
                     /locus_tag="K8M77_002442"
                     /pseudo
     CDS             complement(1034..1288)
                     /locus_tag="K8M77_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1300..1488)
                     /locus_tag="K8M77_002443"
     CDS             complement(1300..1488)
                     /locus_tag="K8M77_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086882.1"
                     /translation="MKNIDNYKSNKEARNKILKESSEFVADEMRKLQEQDDEKKREEG
                     IKETSEKLANYMRELEEK"
     gene            1850..>2595
                     /locus_tag="K8M77_002444"
     CDS             1850..>2595
                     /locus_tag="K8M77_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBI7086883.1"
                     /translation="MNLFKRFYISLFTPKRYKELIKTSGISTFFYLLILSLLLGLLAF
                     SRNYTELNRFLISRKSAFENKIPPFEIKDGVLDLKDSNFVVFQEENWTFVLNDKEPAK
                     DLLKDYESGIAFGNESLIIKYDNNKITEANYSNMNLSLNDNDLKNSINSLERTFGNTY
                     LSLMVIIFIAFSYIITLILAIITKILCSIRNNPIGFLEIIKLSTYGITPCLVLLALLN
                     LLSLNMLITLPLSFLVSIIYAYFGINYLNK"
BASE COUNT         1026 a          341 c          281 g          947 t
ORIGIN      
        1 gggaggtgac tgttgttata aatctataaa aataatattt ataagatctt agtatggtta
       61 aaatgtttgt gtttaggata aaatattgtt agctttatag ttttcaaaat ctgactcgta
      121 gaacagggaa ttaaacaaga tttataaata tttatagatt tttggcaacg ggctattaaa
      181 tgaataaaaa aaatatttta gtacttgtag gatcattgat atgtgtagct ttattaggtt
      241 attttacata ctcaacggtt tctacattaa tgaaatctaa atccgtttct caagaggctt
      301 ctgttactaa agatacctcc tctgagaata aagataaaaa ctcacaagat gatttaagtg
      361 aaagattaaa agaaactaac gacaagaagg aagaaaataa aaacaatact gatgaaaaga
      421 aatcagaaaa aactgctaat agttcagaaa ataaatctgc taatgataaa tcaaataatt
      481 ctactaatga aaacaaaaaa aatacttctg ataaaaatac taattcaaaa gaaacattaa
      541 ataatgataa aaacaaatct gaaaactcta aatcagaaag taaaaataca ataagctact
      601 cttcttactt gaaagattta gaaacagaaa aatatactct taaaaatgat gaaaaattaa
      661 gtgacgtggc taaaaagttt aaggatactt gtactattaa ttctagtttg aatattataa
      721 aatctctcaa tcaaataagc aatacttcat cactagaatc aggggataca gtattagttc
      781 ctgttaacgc ttttcaagat gggaaaaaat actctgtaaa agaaggggat acttggtata
      841 gcatagctag aaaacactac cctaaatata atcatgaggt agttataaat ttccttatgg
      901 atataaaccc atttaaaaat gatattcttc cattaggtga agatatattc ttacctaata
      961 tttaaatagc ttaccactta aataaaaatc ttaaccttct ttaagataag gttaagattt
     1021 ttaaatttat ttttatatat aaagttaagg ttaataaatc atccatcttt tcaccatctt
     1081 taaagcttac tgaaaatgga agatgtttat ccatcaactt aactgttatt aggtttagaa
     1141 taattattac aaataattta gctataattt gcggaataat tcttaatcca aatacccata
     1201 aatatactaa agccaatact atatatacag gcagaataat tttataaaac acacctttat
     1261 ataccccttt aaacattgaa gccatatctt atactaaaat tatttttctt ctagctctct
     1321 catatagtta gctaactttt cagaagtttc ttttatacct tcttctcttt tcttctcatc
     1381 atcttgttct tgtaactttc tcatttcatc tgcaacaaat tcagaagatt ctttaagaat
     1441 cttatttcta gcctctttat tacttttgta attatcaata tttttcatct tcttatcctc
     1501 ttttctaaga ttatttaaag taaaatcaaa cctattaagt aatttattaa tgaagttttt
     1561 taaaagaaat tttaaaattc ttttttcaat ttcaagacct atttaaatac actaaatacc
     1621 ctatcaaaca aataactttt caagtaaaat tttacattat agcattattt tacaccacca
     1681 ttcttattca cacaatatta atatttcatt aaattattta caaattttag cgatatataa
     1741 atttatattt cacgaaaaat attaatttta gtttagtata tatccaccct ttaaattaat
     1801 aaatttctat ttaatgaaca aactaataaa aaatagaaag ggtgattata tgaacctatt
     1861 taaaagattt tatataagtt tatttactcc taaaagatat aaagaactta ttaaaacttc
     1921 tggaataagc actttctttt atttacttat tttatctctt ttattaggac ttttagcttt
     1981 tagtagaaat tacactgaac ttaatagatt tttaatttct agaaaatctg cctttgaaaa
     2041 taaaattcct ccctttgaaa ttaaggatgg agttttagac ctaaaagatt ctaacttcgt
     2101 agtttttcaa gaagaaaatt ggacctttgt actaaatgat aaggaacctg caaaagactt
     2161 attaaaggat tatgaatcag gaattgcctt tggaaatgaa tctttaataa ttaaatatga
     2221 caataataaa attactgaag ctaattattc aaatatgaat ctatctttaa atgataatga
     2281 cttaaaaaat tctataaata gtttagagag aacatttgga aatacatatt tatccttaat
     2341 ggttattata tttatcgctt tctcatacat tataacctta atattagcta taataactaa
     2401 aattctatgt agtataagaa ataaccctat tggatttttg gaaataataa aactaagtac
     2461 ttatggaata actccatgct tagtattatt agcattacta aatttattaa gcttaaatat
     2521 gctcattact cttcccttaa gctttttagt atcaataata tatgcttact ttggaataaa
     2581 ttatttaaat aaaaa
//
LOCUS       DAEVGI010000230         2572 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEVGI010000230 DAEVGI010000000
VERSION     DAEVGI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.232"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            118..774
                     /locus_tag="K8M77_002445"
     CDS             118..774
                     /locus_tag="K8M77_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HBI7086884.1"
                     /translation="MNKEKLKSVFTSKIAIGIICFIIGGIALGNSNSGVNLTKERYDQ
                     LLEFEKVALQKDGETVETLNEELTEENLDNKINEKTEEDKRPLSIGETAYINDENGVK
                     SMAITIDNVKLTDERNQFSEKEAEKVVVIEYTYENIADEENLYVFDSNFKVYDASGNV
                     LETYPAGAEKYPQAISKGKKCTANMSFALNNESNNLEIEFYDNMFDGKASEVFNISAE
                     "
     gene            851..1402
                     /locus_tag="K8M77_002446"
     CDS             851..1402
                     /locus_tag="K8M77_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HBI7086885.1"
                     /translation="MRYSVEGVCEKFKSGEKLEFLFFWGHTKNKNGKITKACFSQWYL
                     SDFTVNGVLYNCAEKYMMAEKARLFKDYETLEEILSAENQKEIKDLGRKIKNFNEELW
                     NREKYEIVKRGNLAKFSQNENLKEFLLNTGDKIIVEASPYDSIWGIGMGAKDENIEDP
                     TAWKGENLLGFALMEVRDLLNTM"
     gene            complement(1608..2489)
                     /locus_tag="K8M77_002447"
                     /pseudo
     CDS             complement(1608..2489)
                     /locus_tag="K8M77_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452629.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1008 a          240 c          419 g          905 t
ORIGIN      
        1 cctcggagag ttatatcaaa ggaaagtaga ttactattat agttttcaaa atcggactcg
       61 tagaacaggg aattaaacaa gatttataga tatttataga tttttggcaa cggtaatatg
      121 aataaagaaa agttaaaatc agtatttaca agtaaaatag ccataggaat aatttgtttt
      181 attattggag gaattgcttt aggaaacagc aattcaggag ttaatttaac taaagaaaga
      241 tatgaccaat tattagagtt tgaaaaggtt gctcttcaaa aagatggtga gacagttgaa
      301 actcttaatg aagaattaac tgaagaaaat ttggataata agataaatga aaaaactgaa
      361 gaagataaaa gaccattaag tattggagaa acggcttata taaatgatga aaatggagtt
      421 aaatcaatgg ccataaccat agataatgta aaacttacag atgaaagaaa tcaattttct
      481 gaaaaagaag cagaaaaggt tgttgtaata gaatatactt acgagaatat agcagacgaa
      541 gaaaatttat atgtatttga tagtaatttt aaagtttatg atgcttcagg aaatgtttta
      601 gaaacatacc cagctggtgc tgaaaaatat cctcaagcta ttagtaaggg gaaaaagtgt
      661 actgctaata tgtcatttgc cttaaacaat gaatcaaata atttagaaat agaattttat
      721 gataatatgt ttgatggaaa ggcttcagag gtctttaata taagtgctga atagaagtta
      781 ttttaagaaa aaagtctatt taaagtttta tagttaattt agaatataag taaggtaagg
      841 gggaggtatt atgagatatt ctgtagaagg tgtttgtgag aagtttaaaa gtggagagaa
      901 actagagttt ttatttttct ggggacatac aaaaaataaa aatggaaaaa taactaaggc
      961 ttgttttagc caatggtacc tttctgattt tacagttaat ggagttttat ataactgtgc
     1021 agagaagtat atgatggctg aaaaggcaag gttatttaag gattatgaaa ccttagaaga
     1081 aatattatct gctgaaaatc aaaaagaaat aaaagactta ggaagaaaga ttaaaaactt
     1141 caatgaagag ctttggaata gagaaaaata tgaaattgtt aagaggggaa acttagctaa
     1201 gttttctcaa aatgaaaatc ttaaggagtt tttattaaac actggagata aaataatagt
     1261 tgaagcaagt ccatatgact ctatttgggg aataggaatg ggtgcaaagg atgagaatat
     1321 agaagaccct acagcttgga agggggaaaa tctcttaggt tttgctctta tggaagtaag
     1381 agacttgcta aataccatgt aaaaagcttt aaagaaaaaa tttcaatatg tttcttttat
     1441 taaaatttag atagtgtatt ttaaaattat gaaaatatat taattttaaa aagggcatgt
     1501 atcaaaacaa acttttaata aaaattacaa attcttaggg aggtttaagg attctctaaa
     1561 tatttttgtg atttttctta ttgttttttg atatagccct ttttctttta tccttcaaat
     1621 ttagtttttt ctatttcatt tagtatttta actatattac ctaagtcata ttgtactgct
     1681 aaatttgaaa atatataaat attattatag tttttgggta tgtaattagg atcgaagttt
     1741 gtaattacaa gatcataatt ttctttttta tattcatcat aaacttccac aataacatca
     1801 aattttcttc ttaaaaattt tcttaaatag tcaatagtgg tgttaagaat caaactatct
     1861 attctagata aaatacctat agtaagggaa tgtttggaca ctccattaag ataatttaag
     1921 gttaatatta tgcaaagttt aaagtaatct ggatcaaagg aatattttac tttatataaa
     1981 gagttaattt ttaataataa gtcattaaca aatttttctc tagaatcact tgtgaattca
     2041 tttaaataat aatttgtaac aattttattg tttgaataaa aaattccctt aaagaagtaa
     2101 cttttaaagc ataaacttag taaatgcaat ttcacttcat tattgaagtt attagggtga
     2161 gtggtatata agttactcat ttcctcagtc attaaagaga aaatcttaaa gggagtactt
     2221 tcattaactg tagaaaattc tttaataact ttatatgaga ttatgttaaa acttaaaata
     2281 aagcaaagga tagaatatgc taagtaactt aattaaggaa tctttttcaa agaaattttt
     2341 aagctctata aaaatttcag tgttttctaa tattcttaaa tttttttctt atcatcaaaa
     2401 tttttaacca attatataaa ggatactttt cactaatttt tatggtaata ttaaaagttt
     2461 tttattactt cagttatgtt gtgattagct taatatgaag ttcttagaaa gttacaagtt
     2521 aaaactgaaa aaaactggtt ttatttatgt gaaaatcagt ttttattttt at
//
LOCUS       DAEVGI010000231         2564 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEVGI010000231 DAEVGI010000000
VERSION     DAEVGI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.233"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(276..953)
                     /locus_tag="K8M77_002448"
     CDS             complement(276..953)
                     /locus_tag="K8M77_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI7086886.1"
                     /translation="MKKKSLIILSMIVLIFVCITLYLTREHIMVFYKLITNGEVIKSY
                     LERFGPWAAIIFFIFQILQVVIFFIPGEVIQAAGGYIFGALGGTLLSFFGIAVGSAIL
                     FYICQKFGKKLVQRFVPKDMYDKLEKILNSPKFKLILVILFFLPGAPKDSLIMVCALS
                     NITIGHFIIFSMIGRIPALVASSFMGANLAEGNIVGVIIISVIALIACALGVIFRKDV
                     FKYMEKL"
     gene            complement(1398..2246)
                     /locus_tag="K8M77_002449"
     CDS             complement(1398..2246)
                     /locus_tag="K8M77_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBI7086887.1"
                     /translation="MNFDIALDFLKRLKRIYFSILILITLIALGFLLYPSFSNYINNK
                     FAVSTISDYTEKINNVKDEEVDNLIKNINKYNYDLFNGTAENQLPDYLNIHEGDILGY
                     IEIPSINIKLPIYYGTSVDILKKGVGVLDGTSLPVGGENTHSVLSAHTGLANQKLFTD
                     IDKLKDGDVFYLHILKKDLAYKVNQIKVVHPDEIDELKISDDKDYVTLLTCYPYGINT
                     ERLLVRGERTDLSPSNVEQVQKEISTFNHSNENLILIVIILNSVLIIIFLLFLIMKFK
                     GKNKSR"
BASE COUNT          985 a          380 c          225 g          974 t
ORIGIN      
        1 ccaagattaa atcataaatt atattttaat gtctctattt acaatattaa ccatttattt
       61 tttctttaat acatataaaa tccctatcat taaaatattt ttatactttt aaacttaatt
      121 tcaaaataat acttgtaatt aataaactct tataatgttt ctttaaaaat aatattaact
      181 taaagtaata taaaaaatgg agttattaaa aaactcttat acaaaattat aaattttata
      241 tatgaatttt tataaatctc catttaaata aaatattaaa gcttttccat gtacttaaat
      301 acatcttttc tgaatataac ccctaaagca caagctatta gggctattac gctaataatt
      361 attactccta caatattacc ctctgctaag tttgctccca taaaagaaga tgcaactaaa
      421 gccggtattc ttcctatcat agagaatatt ataaagtgtc ctatggttat gttacttaag
      481 gcacaaacca taataagtga atcctttggt gcccctggta aaaagaatag tattaccaat
      541 ataagcttaa acttagggct atttaaaatc ttttctaatt tatcatacat atccttaggc
      601 acaaaccttt gtactagctt ctttccaaac ttttgacata tgtaaaataa tattgcagat
      661 ccaactgcta tgccgaaaaa tgaaagtaaa gttcctccta aggcaccaaa tatatacccc
      721 ccagcagctt gtataacctc tcctggaata aagaatataa ctacctgtaa tatttgaaag
      781 ataaaaaata ttatagctgc ccaagggcca aacctttcaa gataactctt tataacttca
      841 ccattagtaa ttaatttata aaaaaccatt atatgttctc tagttaaata aagagttata
      901 catacaaata ttaaaactat catacttaaa attattaaac tcttcttctt cataaataaa
      961 cttaatcgta gattttcctc cttaaatagt ttttaaattc tattttaaat aagtatttat
     1021 tctatctatt attttctaaa acaaagaaat atttaaaata aaattactgt ctaaagttaa
     1081 tataaattaa aattgtatat attctaaatt taaccacatt aactcttttt tatgtaaata
     1141 acttggaatt ttttacatct attataactc ataatactaa acttagatat tataataatc
     1201 ttaaatttat attacatttt tatttaaaaa tgaagtgtaa taaaacatat aaaaataaat
     1261 tttagtagct cttaaaaaat atatctttag actaattttt ttcaagtact ctaaacctat
     1321 aaacaactct caattagtaa tcttaatact tttatataat taattaaaga atttaaatta
     1381 tattatttat aaaatagtta tctactctta tttttccctt taaatttcat aattaaaaac
     1441 aataagaaaa taattattag cactgaattt aatattataa ctattagtat taaattttca
     1501 tttgaatgat taaaagtgct tatttccttt tgaacctgct ctacattact tggacttaaa
     1561 tcagttctct ctccccttac taataaacgt tcagtattaa ttccataagg ataacaagtt
     1621 agtaaagtta catagtcctt gtcatctgat attttcagtt catcaatttc atctgggtga
     1681 acaactttta tttgattgac tttataagca agatcttttt ttaaaatatg caaataaaaa
     1741 acatctccat cttttagttt atctatatca gtaaaaagtt tttgatttgc taatccagta
     1801 tgagctgata aaactgaatg agtattttcc cctcctacag gaagtgatgt cccatctaaa
     1861 actccaactc ccttttttaa aatatcaact gaagttccat agtatatagg taatttaata
     1921 tttatactag gaatttctat gtaccctaag atatcccctt catgaatatt taagtaatct
     1981 ggcaattgat tttcagcagt tccattaaat aaatcataat tatatttatt aatatttttt
     2041 attaaattat caacttcttc atccttaaca ttatttatct tctctgtata atcagaaatt
     2101 gtagaaactg caaatttatt atttatgtaa ttagaaaaac taggatataa caaaaatcct
     2161 aaagcaataa gtgtaatcaa aattaaaata gaaaaatata ttctttttag ccttttcaaa
     2221 aaatctagtg ctatatcaaa gttcattagt tataggttat tttgatatgc accaactcac
     2281 ctcctaaagt ttaattaacc taaaagacgc aaatcttcat gtcataaaaa aacttctttt
     2341 tataatttaa attaaaaaga agtttttatt tatatgctct gtatcaaaat acttttataa
     2401 aacttacaaa catattccga gaattactag atttcgctaa gaatttataa ttattacttc
     2461 gaagatatta ctaaagcttc gaaacttaag tgaaagccca aatttttaat ttggaatctt
     2521 gaacttatgc aatcgaactt tgtgagattg tattcctaag aaat
//
LOCUS       DAEVGI010000232         2542 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEVGI010000232 DAEVGI010000000
VERSION     DAEVGI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.234"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(199..864)
                     /locus_tag="K8M77_002450"
     CDS             complement(199..864)
                     /locus_tag="K8M77_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086888.1"
                     /translation="MTKKKNKLFIVILSILILISACFFGVKAVYKSYFAPKLSVTSIN
                     SNIELNNWNLIKKFLPNNINLSLKEINVSSVTEFSDSELTDLFILALREEPNAIKDLT
                     GLKVDIENNDINIYVDVNYKDIPFQGKLTFTAQSKDGKGIFHYKEGKVGFIDISKDTI
                     FKNLQDNSILSFDKTNGDIILSFKDIIKYLQIKSIKVENNKIVLVFNGTIPLLSSIFP
                     NLN"
     gene            1007..2005
                     /locus_tag="K8M77_002451"
     CDS             1007..2005
                     /locus_tag="K8M77_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086889.1"
                     /translation="MIKTDKGKIIDYKLGDVTGDGINDEVYIMSCAFNKCLNRHWLVV
                     KEGNSERIFKYELTENNYNFEIFLVKFRHTNKLDIFIRSIGECFGGCVNGKIFTYDRE
                     NLKEIFNTNDFYKKNKLSAFYRDDYKVDVLNYERNKKYIVDIKENFKYYLDFVYYGDG
                     KVKEGKDKANISSVWGCNLYYPMGSEVSNISIIQKVIGQASTDDICLIESILKWQDNS
                     FRVIEQNVILKGAFINKNNRIKEISNKKDFFIDKRNCYENNLKGLNKYINDNEDFDSS
                     ITWYYLAILQFFEKDLIEALKSINMNLSFKHPYPSKERALTLKEEIEYSIKSNMFI"
     gene            complement(2074..>2542)
                     /locus_tag="K8M77_002452"
     CDS             complement(2074..>2542)
                     /locus_tag="K8M77_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HBI7086890.1"
                     /translation="EKITDRTPSEALNDNLKGLYMGIDIFKRYETKCKSNDLKNLLND
                     VINLYSKEACELSNKISALGDSPSSNVGILGKVSEAIYNIKTITADTDSEILEESIKA
                     FKTGIIMFQKFLQEKENQLDKESISLVKNIIRENEDISKKLNEYSSPHQANFF"
BASE COUNT          961 a          250 c          347 g          984 t
ORIGIN      
        1 tgttaacttc ttaggaatac aatctcacaa agttcgatta cataagttca agactccaaa
       61 ttcaaaattt ggactttcac ttaagtttcg aagctttagc aatatcttcg gagtaataat
      121 tataaattct tagcgaaatc tagtaattct cggaatatgt ttataagttt tattattata
      181 ttttgataaa gccatttttt agtttaggtt tggaaagatt gagctaagta atggaatagt
      241 accattaaat actagtacta ttttattatt ttctaccttt atagatttta tttgaaggta
      301 ttttattata tccttaaatg aaaggattat atctccatta gttttgtcaa aggaaagtat
      361 tgaattatct tgtagatttt taaagatagt atccttagaa atatcaataa agccaacctt
      421 tccttcttta tagtggaata ttcctttacc atccttactt tgagctgtaa aggtaagttt
      481 tccttggaaa ggtatatctt tatagtttac atctacataa atattaatat cattattttc
      541 tatatcaacc tttaatcctg taagatcttt tattgcatta ggttcttcac gcaaagctag
      601 tataaataaa tctgttaatt cagaatcaga aaattcagtt acagaactaa catttatttc
      661 ctttaaagaa agatttatat tgttaggaag gaattttttt attaaattcc aattgttaag
      721 ttctatgtta gaatttattg aggttacaga taatttagga gcaaaatatg acttataaac
      781 tgcttttact ccaaagaaac aagctgatat taaaattaat attgataata taactataaa
      841 aagtttattt ttctttttag tcatgttaat tatctcccat ctataagatt ttattaattc
      901 aattataaac taaattaatt taaatttcac ttgttttcaa atgaaagtaa cagatgatgg
      961 aagagaatat aaaataatat tataactata taaattagga gttaatatga ttaaaactga
     1021 taagggaaaa ataatagatt acaaattagg ggatgttaca ggagatggta ttaatgatga
     1081 agtttatatt atgtcttgtg cttttaataa atgtttaaat aggcattggt tagttgttaa
     1141 agaaggaaat tcagaaagga tctttaaata tgaactaaca gaaaataatt ataattttga
     1201 aatatttcta gtaaaattta gacatactaa taaattagat atatttatta gatctatagg
     1261 ggaatgcttt ggtggatgcg ttaatggtaa aatttttact tatgatagag aaaatttaaa
     1321 agaaatattt aatactaatg atttttacaa aaaaaataag ttaagtgctt tttatagaga
     1381 tgattataaa gttgatgtac ttaattatga aagaaataaa aaatatattg ttgatataaa
     1441 agagaatttt aaatattatt tagactttgt atattatgga gatggaaaag tgaaagaagg
     1501 aaaggataaa gcaaatataa gtagtgtatg gggctgcaat ttatattatc ctatgggaag
     1561 tgaagtttct aatataagta ttatccaaaa ggttattggt caggcttcta ctgatgatat
     1621 atgtttaatt gaaagtatat taaaatggca agataattct tttagagtaa ttgaacaaaa
     1681 tgtaattcta aaaggtgctt ttataaataa aaataataga attaaagaaa taagtaataa
     1741 aaaagatttt tttattgata aaagaaattg ttatgaaaat aacttaaaag gattaaataa
     1801 gtatattaat gataatgaag attttgattc atcaattact tggtattacc tagctatatt
     1861 acaatttttt gaaaaagatt taattgaggc tttaaaatca ataaatatga atttaagttt
     1921 taagcaccct tatccttcaa aagaaagggc tttaacttta aaagaagaaa ttgaatatag
     1981 tattaaatca aatatgttta tctgagaaat tattaaagta ttttataata aattttaact
     2041 tataaaatag gctatttata tagcctattt tatttagaaa aaattagctt ggtgagggct
     2101 tgaatactca tttaattttt tgctaatatc ttcattttct cttataatat tttttacaag
     2161 agaaattgat tctttatcta attgattttc cttttcttgt aaaaactttt gaaacataat
     2221 tatacctgtc ttaaaagctt ttatgctttc ttctagaatt tcactatctg tatcagctgt
     2281 aatagttttt atattgtaaa tagcctcaga aacttttcct aaaattccta catttgaaga
     2341 tggtgagtca ccaagagcag atattttatt tgaaagttca caggcttctt tagaataaag
     2401 atttataaca tcattaagta aattttttaa gtcattagat ttacattttg tttcgtaacg
     2461 tttaaaaata tcaattccca tataaagccc cttaagatta tcgtttaaag cttcactagg
     2521 agttctatct gtaatttttt cc
//
LOCUS       DAEVGI010000233         2524 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEVGI010000233 DAEVGI010000000
VERSION     DAEVGI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.235"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            169..1206
                     /locus_tag="K8M77_002453"
     CDS             169..1206
                     /locus_tag="K8M77_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBI7086891.1"
                     /translation="MSGVAGEGCEILVPFNERRPLAEIERSLVKTYRKQVWSKFIKAI
                     KDYKLVEEGDKIAIAISGGKDSLIMAKLFQELKKHGQINFELVFLAMDPGYHPQIREL
                     LEDNCKYLDIPVHIYDSGIFQVVDKIAKDYPCYMCARMRRGSLYGKAKELGCNKLALG
                     HHFNDVIETTMLNVLYAGNFKTMLPKLKAANFDGIELIRPLYYIEEKYIKRWIQRAGI
                     WPLNCACMVAAKKIGNKRFEIKDMIEEFKEKFDGVDKSIFRAAQNVSVDSILGWDIDG
                     EHYSYLDFYDEESLLNSTAELKQKGLVNEAVAYERQNIARNLIGLLDDEVIAEKTGLS
                     LKEIKSMQIDN"
     gene            1486..1977
                     /locus_tag="K8M77_002454"
     CDS             1486..1977
                     /locus_tag="K8M77_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7086892.1"
                     /translation="MQDILYYIHTYGLIVLFLMVVGEYCVPAIPTGIVMPAAGIIAGQ
                     AHKEIINVMLISLIAGVVGSIILYFIGYFLGSTILEKLKIKHPKIEDKLEKTEKILEK
                     HSFLGIFICRLIPIIRTYVSLICGTVKVNFLKFIIFSIPGIFCWNFTGIVVGYFFGGK
                     IRF"
     gene            2048..>2524
                     /locus_tag="K8M77_002455"
     CDS             2048..>2524
                     /locus_tag="K8M77_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7086893.1"
                     /translation="MKGERESFMDIDAVLNYFATYGLTLLFVIVFLEYLNLPGLPAGI
                     IMPAAGILASRNNQSIVLVVFISVVAGVLGSIVLYLIGYYGGAILLDKLGLKFPKVQP
                     TIRKSSTILKEKGLFGVFLCRLLPVLRTIVSIIAGSVRMNFFKFLIYSTPGIFLWNL"
BASE COUNT          949 a          225 c          466 g          884 t
ORIGIN      
        1 atctatataa gttatagaaa ttaaacttaa aaacttatat aggtgttttt actaaatagt
       61 ttgtaggtct atttaaatat taaaagtatt taagtattat tttataaaga caaaaagaga
      121 taaaattgtt ataataacaa aagagtttta tatagtgagg tgattaaaat gagcggtgta
      181 gcaggagaag ggtgtgagat tttagtacca tttaatgaga gaagaccttt agctgaaata
      241 gagagaagtt tagttaaaac atatagaaaa caagtttggt caaaatttat aaaagctata
      301 aaggattata aattagtaga agaaggggat aagatagcta ttgctatttc aggaggaaaa
      361 gattctttaa taatggcaaa gctttttcaa gagttaaaaa aacatggtca aataaacttt
      421 gaacttgtgt ttttagctat ggatccagga tatcatcctc aaatcagaga attgcttgag
      481 gataattgca aatacttaga tataccagta catatttatg attcaggaat attccaagtt
      541 gtagataaaa tagctaagga ttatccatgt tatatgtgtg caagaatgag aagaggatca
      601 ttatatggta aggcaaagga acttggatgt aataagttag ctttagggca tcattttaat
      661 gatgttatag aaacaacaat gcttaatgtt ttatatgcag gtaactttaa aaccatgttg
      721 ccaaaattaa aagctgctaa ttttgatgga atagagctta taagacctct atattatata
      781 gaagaaaaat atataaaaag atggattcaa agagctggaa tatggccatt aaactgtgca
      841 tgtatggtag ctgctaaaaa aataggtaat aagcgttttg aaattaagga tatgattgag
      901 gaatttaaag aaaagtttga tggagtagat aaatcaatat ttagagcagc acaaaatgta
      961 agtgtagatt ctattttagg atgggatatt gatggagaac attattctta tttagacttt
     1021 tatgatgaag aatccttatt aaattcaacg gctgagctaa agcaaaaagg actagtaaat
     1081 gaagctgttg cctatgaaag acaaaacata gctagaaatc ttataggact attagatgat
     1141 gaagtaatag ctgagaaaac aggattatct ttaaaagaaa taaaaagtat gcaaatagat
     1201 aattaaaaaa tgggatgtat taagacaaaa agataaaact tataaatgag tttttacaat
     1261 tattaaattt ataattgtaa aattataata ggttatatta taaagctact ttagataata
     1321 tatttataat atttataagt tttatagtag attttgtctt tatacaccct ttttattttg
     1381 tatttactaa ataggaataa taaggattaa aagtggttat atgttataat attatatatt
     1441 gacttggtaa tattaagtaa ggaaagaatg gtggtattta tgaccatgca agatattttg
     1501 tattatatac atacatatgg gttaattgta ttatttttaa tggttgtagg tgaatattgc
     1561 gttccagcaa tccctacagg tatagtaatg ccagctgctg gtataattgc aggacaagct
     1621 cataaagaaa taataaatgt aatgctaata tccttaattg caggggtagt aggaagtata
     1681 atattatatt ttattggcta ttttttagga tcaactattt tagaaaagct taaaattaaa
     1741 catcctaaaa tagaagataa gcttgaaaaa acagaaaaaa tactagaaaa gcatagtttt
     1801 ttagggatat tcatatgtag attaatacct attattagaa cttatgtatc cttaatatgt
     1861 ggcactgtaa aggttaattt tttaaagttt attatttttt ctataccagg tatattttgc
     1921 tggaacttta caggaattgt agttggatat ttctttggag gaaagataag attttagttt
     1981 gtgattttga ttttgtgtat tgaattttat ttagatttta ggggaaaata ttaaattaag
     2041 gggttatatg aaaggagaaa gagaaagttt tatggacata gatgctgtgc taaattattt
     2101 tgcaacttat ggtttaacct tgttatttgt aatagtattt ttagaatatt taaatctacc
     2161 aggactaccg gctggaataa taatgcctgc tgcggggatt ttagcttcta gaaataatca
     2221 aagtatagtt cttgtagttt ttatatcagt tgtggctgga gttttaggaa gtatagtact
     2281 atatttaata ggatattatg gaggggcaat actcttagat aaattaggat tgaagtttcc
     2341 taaggttcag ccaaccataa gaaaaagcag tactatttta aaggaaaaag gactttttgg
     2401 agtttttctt tgtagactat tgcctgtttt aagaaccata gtttctatta tagcaggttc
     2461 agtaagaatg aatttcttta agtttttaat ctattctact ccaggaatat tcttgtggaa
     2521 ttta
//
LOCUS       DAEVGI010000234         2521 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEVGI010000234 DAEVGI010000000
VERSION     DAEVGI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.236"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            40..2100
                     /locus_tag="K8M77_002456"
     CDS             40..2100
                     /locus_tag="K8M77_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7086894.1"
                     /translation="MRKEYVNFPSDIPVTISYVNIKNYPLHWHDAIEILYVLKGSIKV
                     DIDTDSYEIQEDEIEIVNTEQTHRIYSNKDNRVLIFKIDPHFFEKYYSDIENMFFYTN
                     TSDEGAQSDESYDKLRVFLSIILCEEAQKVDDYDKYIEKSLVELLFHLLNNFHYLLYD
                     NDEIHENNMLLERYHRISKYIYNNYNKNITLKDIANTEFLSTHYLSHEIKYATGLSFT
                     DLLNLTRVEESVKLLLDTDKSLSEISYEIGFSHTRYFNKHFKAYYNCTPLQFRKKHKI
                     SEEEYNKQKEITYYPLTDSLEELSYYLDDYPRFNYEDKIHKLTFNMNTEGTEFNKYFK
                     EVLNVGDAFDLLLEDNQDIVEDLQDHIGYNYIRLLHVFSSDMGIFPGSKFFNWTRTFD
                     IFEYISSLDLIPLIVLDDFGYSKDNFLDAIKSFIDFFSEVESFELTDLKFQFTSTFNE
                     ELKNSLIELFESRDLTLVNELYTPNNKIDLIYDTAYMLPFIIHNTVSSGSKLNFIKAF
                     DALDRQIDITNEVFFGYPAMVNDKGIKKPSYYAYYFLSKLGDTLLYKGDGYILTKSED
                     EYQLLVYTYNDEIDSLIDFKNFTKLRGVKDLVDKKLSLNLLDLDSDVRITKYTIGENF
                     GSSFNYWLSMGKPKRLRKAEKDILFQASYPKIEFKYAKKNTILNIQTTLQGYCAELFI
                     LKKV"
BASE COUNT          984 a          324 c          334 g          879 t
ORIGIN      
        1 gaatttagtt taatcctatt atacaaagga gttttttaag tgagaaaaga atatgttaat
       61 ttcccttcag acatacctgt aaccatatct tatgtaaaca taaaaaacta tcccttgcat
      121 tggcatgatg caatagaaat actttatgtc cttaaaggca gcataaaagt agatatagat
      181 actgacagtt atgaaataca agaagatgaa attgaaatag taaacacaga acaaactcat
      241 agaatttatt ctaataagga taacagagtt ttaatattta aaatagatcc acactttttt
      301 gaaaaatact atagcgacat agaaaatatg tttttctata ctaatacctc tgatgaaggt
      361 gctcaaagtg atgaatctta cgataaactt agagtatttt tatccattat attatgtgaa
      421 gaagctcaaa aagtagatga ttatgataaa tatatagaaa aatctctagt agagctttta
      481 ttccaccttt taaataattt ccactatctt ttatatgata atgatgaaat tcatgagaat
      541 aatatgctcc tagaaaggta ccatagaatt tctaagtata tatataataa ctacaataag
      601 aatataacct taaaagatat tgctaatact gagtttctta gtacacatta tctttcccat
      661 gaaataaaat atgcaacagg actaagcttt acagaccttc taaacttaac tagagttgaa
      721 gaatcagtaa aacttctttt agatactgat aagtccttat ctgaaatatc ttatgaaata
      781 ggcttttctc atactcgtta ttttaataaa cattttaagg cctattataa ttgcactcct
      841 cttcaattta gaaaaaaaca taaaataagt gaagaggaat ataataaaca aaaagaaata
      901 acatactatc cattaactga tagtcttgag gaactttctt attacttaga tgattaccca
      961 agatttaatt atgaagataa gattcataag cttactttta atatgaatac tgaaggcact
     1021 gaatttaata agtactttaa agaagttctt aatgtaggag atgcctttga cttattatta
     1081 gaagataacc aagatattgt tgaagatctt caagatcata taggttataa ctatataaga
     1141 cttctacatg tattctcatc agatatgggt atattccctg gttcaaaatt ctttaactgg
     1201 actagaacct ttgatatttt tgaatatata tcttcattag acttaatccc tttaatagtc
     1261 ctagatgatt ttggatattc taaggataat ttcttagatg ctataaaatc cttcatagac
     1321 ttttttagcg aggtagaaag ttttgaattg acagatttaa aatttcaatt tacctctaca
     1381 tttaatgaag aactaaaaaa ttctttaatt gaattatttg aatctaggga tttaactttg
     1441 gtaaatgaac tttatactcc aaataataaa atagatttaa tatatgacac agcttatatg
     1501 cttccattta taattcacaa tactgtaagt tcaggaagca aattaaactt tataaaagcc
     1561 tttgatgcct tagatagaca aattgatatt acaaatgaag ttttctttgg ctatcctgca
     1621 atggtaaatg ataaaggaat taaaaagccc tcttattatg cttactattt tttaagcaaa
     1681 ctaggagata cccttcttta taagggagat ggatatatac taactaagtc agaggatgaa
     1741 tatcagcttc ttgtatatac ctataatgat gaaattgatt ctcttataga ttttaaaaac
     1801 tttactaaat taagaggggt taaagacttg gtagataaaa aactttcctt aaacttactt
     1861 gatttagata gcgatgttag aataactaaa tacaccatag gggagaactt tggctcttca
     1921 tttaattact ggctttccat gggaaaacct aaaagattaa gaaaagcaga aaaggatata
     1981 ttattccaag cttcctatcc taaaatagaa tttaagtacg ccaaaaaaaa tactatttta
     2041 aatatacaaa caactcttca aggatattgt gcagaactat ttatactaaa aaaagtttaa
     2101 tattgtataa taaccatgaa aaaagagtac ttttttgcct ttttcaaata ttttccttaa
     2161 aaactttcct tacatttgta cattttttta aacatacatg caaattattt tattaaggaa
     2221 ataggtatat aatataaata tagaagaaaa gagaagatat gaaagaaggt attttttatg
     2281 gcaaggctaa aaaagttttt tagaccgatt aatttaaatt gttttaattg caaattaagt
     2341 tttttacaac aaacaatgga cttattttat agagattaaa tatatatgaa aagagctaga
     2401 atgttaaatt ctagctcttc tttcatttta aaataaaatt aaggagttct atcaaattaa
     2461 ctttttatac actttacaaa cattatgaag agaatatcta gatgagcttt agaatttaaa
     2521 t
//
LOCUS       DAEVGI010000235         2506 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEVGI010000235 DAEVGI010000000
VERSION     DAEVGI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.237"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            48..1880
                     /locus_tag="K8M77_002457"
     CDS             48..1880
                     /locus_tag="K8M77_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HBI7086895.1"
                     /translation="MHLIPRPKSVINHEGEFFIERDTEIILSSELSFEDLNLAIMTQK
                     EIEKVLDFKLNINKLFMDKKYSNSIILREFKFENEEEYKIEIKENQVIIEGFGAGLFY
                     GCQSFRQLVREFGACIPNLTIEDSPYFKYRGFYHDVTRGMIPTLDTLKSLVDKAAFYK
                     INQLQLYIEHTFAFKGMSEVWMDKDPLTAEEILILDKYCKERHVELVPSLSTFGHLYE
                     ALRSKSFRELCELEIGDEEYSFVDRMAHHTLDVTNPKSLGFVESMLLEFIPLFSSDKF
                     NICCDETFDLGKGKSREKAEKLGVGKIYTEFLNKVYNIVKRFNKNVMFWGDIIVGYPE
                     LLSDIPEDLTCLTWNYHPQANDVATKIIAENNKVQYVCPGVGGWNMMMNLIEGSFSNI
                     RRMVNHGMKYGAIGVLNTNWGDYGNINLLANSMPSMIYGAGISWNPKEEEFNEIFKSI
                     SLMEFGDESMNVVSLMDKLSKNQVAGWGELVRWKEKFNEREETKEEIKNIDTLKVFEG
                     YKVASEVRREFIKLLKNTEDKEAIQSFIVSSKGWELIDKFFMVLLEREFNKKSLLDID
                     KKDLAKDLELWFYDYTSIWRKYNKESELNRIREVIVYMCSYLRG"
     gene            1998..2465
                     /locus_tag="K8M77_002458"
     CDS             1998..2465
                     /locus_tag="K8M77_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086896.1"
                     /translation="MNYWQISAEIGNTNLIDIFFDLGVAIVGPGDKGDYFDNKEEYDI
                     LGKEGIVIKRFAEEVKVGDVLILKQINSKDGSFTIVAAGKVTSPYRFEPVFNKVGLDE
                     LPMQHCRRVKWYIPTDGLVIDKEGLSIGLQKLKDDSPLKIKAQEFMNELKDEN"
BASE COUNT          992 a          229 c          502 g          783 t
ORIGIN      
        1 atgagcaaat ttttgtagaa agtaaaggat tattaggggg aagagatatg catttaatac
       61 caagaccaaa aagtgtaatt aatcatgaag gtgaattttt tatagaaaga gatactgaaa
      121 ttatattaag tagtgaatta tcctttgaag atttaaattt ggcaattatg actcaaaagg
      181 aaatagaaaa agtattagat tttaaactta acataaataa actttttatg gataagaaat
      241 atagtaactc aataatttta agagaattta aatttgaaaa tgaagaggaa tataaaatag
      301 aaataaaaga gaatcaggta ataatagaag ggtttggtgc tggattattc tatggatgtc
      361 agagctttag acagcttgta agagagtttg gagcatgtat tccaaatcta acaatagaag
      421 attctccata ttttaaatat cgtggatttt atcatgatgt aacaagggga atgataccaa
      481 ccttagatac attaaaaagt ttagttgata aggcagcttt ttataaaata aatcagttgc
      541 aactatatat agagcatacc tttgctttta agggaatgag cgaagtttgg atggataagg
      601 atcctttaac agcagaggaa atattgattt tagacaagta ttgcaaagaa agacatgtgg
      661 aacttgtacc atcattatca acctttggtc atctatatga agctttaaga agtaaatcct
      721 ttagagaact ttgtgaatta gaaataggag atgaagaata ttcttttgta gatagaatgg
      781 cacatcatac tttagatgtt actaatccta aaagtttagg ctttgttgaa tcaatgcttt
      841 tagaatttat tcctttattc agttcagata agtttaatat ttgctgtgat gaaacctttg
      901 atttaggaaa aggaaagagt agagagaagg ctgaaaaatt aggagtaggt aaaatatata
      961 cagagttttt aaataaggta tacaacattg taaaaaggtt caataaaaat gttatgttct
     1021 ggggagatat aatagttgga tatccagagc ttttaagtga tataccagag gatttaactt
     1081 gtttaacttg gaactatcat ccacaggcta atgatgtagc cacaaaaatt atagcagaga
     1141 ataataaagt acaatatgtt tgccctggtg taggtggatg gaatatgatg atgaatctta
     1201 tagaaggctc ttttagtaat ataagaagaa tggttaacca tggaatgaaa tatggagcta
     1261 taggtgtttt aaatacaaac tggggagact atggaaatat aaatctatta gctaattcaa
     1321 tgccatctat gatttatgga gcaggaattt catggaatcc aaaggaagag gagtttaatg
     1381 aaatttttaa gtctatatcc cttatggaat ttggtgatga atctatgaat gtagtttctt
     1441 taatggataa gctttctaaa aatcaagttg caggttgggg agagcttgtt agatggaaag
     1501 aaaagtttaa tgaaagagaa gaaaccaagg aagaaattaa gaatatagat actttaaaag
     1561 tttttgaagg atataaggtt gcttcagaag taagaagaga gtttattaaa ttacttaaaa
     1621 atacagagga taaagaagct attcaaagct ttattgtgtc ttcaaaggga tgggagctta
     1681 tagataaatt tttcatggta ttacttgaaa gagagtttaa taaaaaaagt ttattagata
     1741 tagataaaaa agacttagct aaggatttag agctttggtt ttatgattac acatcaatat
     1801 ggagaaaata taataaagaa agcgagctta atagaataag agaagttata gtatatatgt
     1861 gctcatattt aaggggttaa gaggattttt aaaggctaaa caaatataaa gttaaagagt
     1921 ataataaatt ataaagtaat aattactttg ttgataactt tatttttata ataatagaat
     1981 gaaagagggt aataattatg aattattggc aaatatcagc agagataggt aacacaaatt
     2041 taatagatat attctttgat ttaggagtag caattgttgg ccctggagac aaaggagatt
     2101 attttgacaa taaagaagag tacgatatct taggcaaaga gggaatagtt ataaagagat
     2161 ttgctgaaga ggttaaggta ggcgatgtac ttattttaaa acaaataaat tcaaaggatg
     2221 gaagctttac tattgtagca gctggtaaag taacaagtcc atacagattt gaacctgtat
     2281 ttaacaaagt aggactagat gaattgccta tgcagcactg tagaagggta aaatggtata
     2341 ttcctactga tggcttagta atagataaag aagggttaag tattggatta caaaagctta
     2401 aggatgatag cccactaaaa ataaaagctc aagaatttat gaatgaatta aaggatgaaa
     2461 attaaaaatt aattataaaa attaaaaacc aagctctagc ttggtt
//
LOCUS       DAEVGI010000236         2412 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEVGI010000236 DAEVGI010000000
VERSION     DAEVGI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.238"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            38..898
                     /gene="citG"
                     /locus_tag="K8M77_002459"
     CDS             38..898
                     /gene="citG"
                     /locus_tag="K8M77_002459"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBI7086897.1"
                     /translation="MNINQEVFLEKLNSLIIKSLLYEVSCYPSPGLVSPISNGVHKDM
                     DYYTFLDSISVIGPKFKEIAEISMSDILVSDLLNKIRPIGLKIEEAMFHVTNNINTHK
                     GLIFLMSIVCAATSKAFYEGKPFKEIRNLIKEMTNGLVYKELNSKTNKKNETYGEKIF
                     RLYGITGIRGEVEKGLPAVFDYSLPFYMEHKHLKTNERLYLTLLCIMANCEDSTLLHR
                     NDIYVLEEVKKRSQEILNMIHSKEPEIIEEKIFEIDKEFSERGISPGGSADLLAITVF
                     LDGIKKYFPL"
     gene            947..1912
                     /locus_tag="K8M77_002460"
     CDS             947..1912
                     /locus_tag="K8M77_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI7086898.1"
                     /translation="MKFEVNFEDQYNEFLANISNMYYNENKTQSEIAKEFNTTRFKVA
                     KYLQEARDKNIVNIEINHPEIRLSSLEKKFKEQFGLKEAIILKVNNNDTSEYAQTLGK
                     TAADYIQTILNDDSIVGLTWGKTLYHAIKNIKASKKLPITVVQPFGTSGKRNISVDTP
                     SLVHLLSDKYDSNYRLLYAPLYIMDDMVRKNLSMELVINQSLLKAKEMDILLTGLGTP
                     EAIYSTLLWKESFLQDEFIKHQAVGSVYGRLYDKEGNFLDTNLNNKVFGVDSDTIKAI
                     ENKVCIVTGKFKSVAILGALRGKLINTLITDELTATKVLHLNSIE"
     gene            complement(2038..2265)
                     /locus_tag="K8M77_002461"
                     /pseudo
     CDS             complement(2038..2265)
                     /locus_tag="K8M77_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          972 a          297 c          335 g          808 t
ORIGIN      
        1 tatatttttc atatgttaaa gaaaaggagt ggttattttg aatattaacc aagaggtttt
       61 cttagaaaaa ctaaacagtt taataataaa atccttatta tatgaggtaa gctgttaccc
      121 ttccccagga ttagtatctc caatttcaaa tggagtacat aaggatatgg attactacac
      181 ttttttagat agcatttcag taattggtcc taagtttaaa gaaatagcag aaataagtat
      241 gtcagatata ctagtaagtg atttattaaa taaaatacgt ccaattggat taaaaataga
      301 agaagctatg tttcatgtta caaataacat aaatacacat aaaggtttga ttttcttaat
      361 gagtattgtt tgtgcagcaa catcaaaagc attttatgaa ggtaaacctt ttaaagaaat
      421 aagaaattta ataaaggaaa tgacaaacgg tttagtttat aaggaattaa attcaaaaac
      481 taataagaaa aatgaaactt atggtgaaaa gatatttagg ttatatggta taacaggaat
      541 aagaggagaa gttgaaaaag gacttccagc tgtgtttgat tatagccttc ctttttatat
      601 ggagcataaa catttaaaaa caaatgaaag actatattta accttattat gtataatggc
      661 taattgtgag gattctacac ttttacatag aaatgatatt tacgttttag aagaggttaa
      721 gaaaaggtct caagaaatac ttaatatgat acattctaaa gaacctgaaa tcatagaaga
      781 aaaaatattt gaaattgata aagagttttc agaaagaggc ataagccctg gaggtagtgc
      841 ggatttattg gcaataactg tgtttttaga tggaataaaa aaatattttc ctttataatt
      901 aaattatcat taacttataa ataatctaaa acagaggtat gacaatatga aatttgaagt
      961 aaattttgaa gatcaatata atgaattttt agctaatata tcaaatatgt actataatga
     1021 aaataaaact cagtcagaaa tagcaaaaga gtttaatacc actcgtttta aagtagctaa
     1081 atacttacaa gaagctcgtg ataaaaatat agtaaacata gaaataaatc atccagaaat
     1141 aagattgtcc tctcttgaaa aaaagtttaa agaacaattt ggtttaaaag aagccataat
     1201 attaaaagtt aacaataacg atacatcaga atatgctcaa actctaggta aaacagcagc
     1261 tgattatatt caaacaatac ttaatgatga tagtattgta ggtcttacct ggggaaaaac
     1321 gctataccat gcaataaaga atataaaggc ttcaaaaaaa ctaccaataa cagttgttca
     1381 gccttttggt acatctggaa agagaaatat aagcgtagat actccaagtt tagtacattt
     1441 attatcagat aaatacgatt caaattatag acttttatac gctccacttt acataatgga
     1501 tgacatggta agaaaaaatt tatcaatgga attggttata aatcaatctt tattaaaggc
     1561 taaagaaatg gatattctct taactggatt aggaactcct gaagccatat actctacact
     1621 tttatggaaa gaatcattct tgcaagatga atttataaag catcaagctg ttggatctgt
     1681 atatggtcgt ctttacgata aagagggaaa tttcttagat acaaatttaa acaataaagt
     1741 ctttggtgtt gattcagata caataaaagc catagaaaat aaggtttgta tagtcactgg
     1801 taaatttaaa tctgttgcta ttttaggagc cttaagagga aaattaataa acactttaat
     1861 tactgatgaa ttaactgcaa caaaagtact acatctaaat agtattgaat aataaagcat
     1921 atttaatact ataaaagtta ctatcttagt attaaaatct taaagaggaa atagttttgt
     1981 ttttcacacc aaactatctc ctctttaaaa ttaccccttt ttattaaatc taatttttta
     2041 tgaatatgat acaagtatat tttccttttc actatcaatt ctaaatccac atattccatg
     2101 tctcattaaa gtaatttctt caaggataaa cccatcacta tacaatgagg aataatattt
     2161 caatactaaa aaatcttttt ctggattctc tatgaagttt ttaaatttta aaagcatatg
     2221 atcttctaaa taaaaatcct cttcttctct atccataaaa aatatatata atttttctta
     2281 aaattaataa tccttaaatt tttattctaa ggcaagtaaa gtagtattaa aaattttttc
     2341 tagtcaaaag ttctctatac cctaattcat ttaaaagttt tgcaaaatta gataaatctt
     2401 ttttaaatgc ta
//
LOCUS       DAEVGI010000237         2409 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEVGI010000237 DAEVGI010000000
VERSION     DAEVGI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.239"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..420
                     /locus_tag="K8M77_002462"
     CDS             <1..420
                     /locus_tag="K8M77_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI7086899.1"
                     /translation="FIAQGALYYLIGTILENQLYVKNNKYTKKTKERFLQLKNVITLI
                     ENEYQNPLTLEDLSKASGMSPKYFCRFFHEMINKTPIEYLNYYRIEISCEKLIATELS
                     ITEIALNCGFNDVSYFIKTFKKHKGLTPKQYLKSLKA"
     gene            complement(477..1625)
                     /gene="fucO"
                     /locus_tag="K8M77_002463"
     CDS             complement(477..1625)
                     /gene="fucO"
                     /locus_tag="K8M77_002463"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBI7086900.1"
                     /translation="MVNRIVLNETSYHGAGAIKEIVTEVKGRGFKKAFLCSDPDLVKF
                     GVTGKVTSILDEANLSYELYTNIKANPTIENVKSGVEAYKKSGADYIIAVGGGSSMDT
                     AKAIGIIINNKEFEDVVSLEGVAPTKNKSVPIIAIPTTAGTAAEVTINYVITDVEKNR
                     KFVCVDTHDIPVVAIIDPEMMQSMPKGLTAATGMDSLTHAIEGYITGAAWEMTDMFHL
                     KAIELISKHLRGAVENTPEGREKMALGQYIAGMGFSNVGLGIVHSMAHSLGAVYDTPH
                     GVANAIILPTVMEYNTEETGDKYKYIAAAMGVENTENMTVEEYRKAAVDAVKKLSEDV
                     GIPANLKEIVKKEDIRFLAESAYNDACRPGNPKETSVEDIIALYESLM"
     gene            complement(1792..2013)
                     /locus_tag="K8M77_002464"
                     /pseudo
     CDS             complement(1792..2013)
                     /locus_tag="K8M77_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          810 a          388 c          283 g          928 t
ORIGIN      
        1 tttatagctc aaggggcttt atattattta ataggaacta tattagagaa tcaattatat
       61 gtaaaaaata ataaatatac taaaaagact aaagaaaggt ttcttcaatt aaaaaatgtt
      121 ataaccttaa tagaaaatga atatcaaaac cctttaactc ttgaagattt atccaaagct
      181 tctggtatgt ctccaaaata tttttgtaga ttttttcatg aaatgattaa taaaactccc
      241 atagaatatt taaattacta ccgcattgaa atttcttgtg aaaaacttat tgcaacagag
      301 ctttccatta ctgagattgc tttaaattgt ggatttaatg atgtaagtta ctttattaaa
      361 acctttaaaa aacataaagg attaacacca aaacaatatt taaaatcctt aaaagcttag
      421 taaataaaaa ataccctctt aagcgaaatt tattcactaa gagggtattt ttatttttac
      481 attaaagatt catataaagc tattatatct tcaacagatg tttcctttgg attacctggt
      541 ctacaagcat cattataagc agactcagct aagaatctta tatcttcttt tttaactatc
      601 tctttaagat tagctggtat tcccacatct tctgaaagct ttttaactgc atctactgct
      661 gcctttctat attcttcaac ggtcatattt tctgtgtttt caacgcccat ggcagctgca
      721 atatatttat atttatctcc agtttcctct gtattatatt ccataacagt tggaagaata
      781 attgcatttg caaccccatg aggtgtgtca taaacagccc ctaaagaatg tgccatagag
      841 tgaactatac caagtcctac atttgagaat cccattcctg caatgtattg tcctaatgcc
      901 attttttctc ttccttctgg tgtattttct accgcgcctc ttaaatgttt tgaaattaat
      961 tcaatagctt ttaagtggaa catatctgtc atttcccatg cagcccctgt tatataacct
     1021 tctatggcat gagttaatga atccatacca gttgcggcag taagtccctt tggcatactt
     1081 tgcatcattt caggatctat aattgctact actggaatgt catgagtatc tacacataca
     1141 aattttctat tcttttcaac atcagtaata acataattta tagttacctc tgctgccgta
     1201 cctgctgttg ttggaatagc aataatagga acagatttgt tcttagttgg tgcaacccct
     1261 tctaagctta ctacatcttc aaattcttta ttatttatta taattcctat ggcttttgca
     1321 gtatccattg atgatccacc accaactgca atgatataat ctgcacctga ttttttataa
     1381 gcctctacac cactttttac attctctata gttggatttg ctttaatatt tgtatataat
     1441 tcataactaa gattagcctc atctaatata gaagtaacct ttcctgtaac tccaaactta
     1501 actaaatctg gatcagagca taagaaggct ttcttaaaac ctcttccttt tacttctgta
     1561 acaatttctt taatagcgcc tgctccatga taagaagttt catttaatac aatcctattg
     1621 accatgcttg tactccccct tttattaata aatatattta tttatatatt tattttaaat
     1681 ttataaaaca tactattcta taaatataat gtcttaaaat ataataatat tcacatttat
     1741 aaggattata tttaataaat gtttttaata tcatacaaat aaaataatta actattcaat
     1801 actcatagtt tccatctgtg attttgtaat gccatttctt tttccatagt taatccaata
     1861 atttgtagag gccttaagat gcatgttttt tgatataagc atggtaaccc ttaagttttc
     1921 aggagtgtta ttataaatag actttgataa ctttttcatt ttccatttta caaagatttt
     1981 aaaaggtgtt cctaaaatag cttcaccacc accatttaat taaattttgc tttatatttt
     2041 tcttaagttt ctatagcaat catagataaa atactatgat aataatcttt attaaattca
     2101 ctgcgcttgt aactttgcat ctatctttat aagatttggt acaagtattt atatttttat
     2161 tatacattcc ttatggatta taaattgaat aaaatgtaaa aaatactaga aatgtttttt
     2221 tataaggaat aaaataatct tgaaatataa ttttaagagg tttaatacta taaaatatga
     2281 ttgtggtttt gtaaaggaaa gtaactggtt tcgttatcta aattaactat tagttgaatt
     2341 atatactata taaatagtat ataaaaaaaa ttctcaatta tcagcttaat gtaattgaga
     2401 attttttaa
//
LOCUS       DAEVGI010000238         2392 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEVGI010000238 DAEVGI010000000
VERSION     DAEVGI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.240"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..722
                     /locus_tag="K8M77_002465"
     CDS             <1..722
                     /locus_tag="K8M77_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI7086901.1"
                     /translation="ILYYQPIVDFRNNTIRSVEALLRLKKDGQLLTPNYFMNIIEEAN
                     MMKEITLWVLNKVIKDYNIISCYNNINEENFYISINVSFNEIKDIEFLKKIVKIVNDN
                     KIIKNSICLEIIEKFGVEEVEKIQKNIKFLRENGILIAIDDFVVEYSNLDLLKKIDSN
                     IIKLDKFFADGINDSEISIKVIGFILDICRLSNKSIVIEGIEKKEQVDIIKTFLYEKI
                     YIQGYYFSKPLDINSLKDYTF"
     gene            923..1081
                     /locus_tag="K8M77_002466"
     CDS             923..1081
                     /locus_tag="K8M77_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBI7086902.1"
                     /translation="MYDCTDEKLKLEYARVYILPQKYENLYSIYKGFWKGTIFKDLYR
                     PYKENKGC"
     gene            1094..1345
                     /locus_tag="K8M77_002467"
     CDS             1094..1345
                     /locus_tag="K8M77_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBI7086903.1"
                     /translation="MKALELLDDIQKLQFYAVELNLYLDNFPENRKATEDYKEISSKL
                     SELIERFECEYGPIRNFGDAYVENPVAWIEQPWPWEIKY"
     gene            1645..2211
                     /locus_tag="K8M77_002468"
     CDS             1645..2211
                     /locus_tag="K8M77_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBI7086904.1"
                     /translation="MWYYVKTLEYPINLKCNDLRMAKFLISQYGGPDGELSAALRYLN
                     QRYTMPTNKSKGLLTDIGTEEMAHVEMIATMIYQLMDNASIEDLKKAGLAGYYTDHGK
                     ALYYADAQGNPWTATYIQAKGDVIADLHEDMAAEQKARATYEWLINLTDDQDIKEILR
                     FLREREIVHFQRFGEALMDVQDKIKYKK"
BASE COUNT         1054 a          199 c          358 g          781 t
ORIGIN      
        1 atattttata ttatcaacca atagttgatt ttaggaataa cacaataaga agtgttgagg
       61 ccttattaag attaaaaaaa gatggtcaat tattaactcc caattatttt atgaatatta
      121 tagaagaagc taatatgatg aaagaaatca cattatgggt attaaataag gtaattaaag
      181 attataatat tataagttgt tacaataata taaatgaaga aaatttttat atttctataa
      241 atgtatcttt taatgagata aaagatatag agtttttaaa gaaaatagtc aaaatagtca
      301 atgataataa aataataaaa aatagtattt gtctagaaat tatagaaaaa tttggggtag
      361 aagaggtaga aaaaatacaa aaaaacatta agtttttacg agaaaatgga attctaattg
      421 caatagatga ttttgttgtg gagtattcaa acttagattt attaaaaaaa atagattcta
      481 atatcattaa attagataag ttttttgcag atggaattaa tgattcagaa ataagtatta
      541 aagtaatagg ttttatatta gatatatgca gattatcaaa taaatctata gttatagaag
      601 gaatagagaa aaaagaacaa gtagatataa taaaaacatt tctttatgaa aaaatttata
      661 ttcaaggata ctatttttca aaaccattag atattaatag tttaaaagat tatacttttt
      721 aaaataaatt tatatattaa acaaggcctt acttgattgt aaaaagagtt ttaaaaataa
      781 aactttattt aataattaat aaggtctttt gttattataa aaatttaatc ttattaaaga
      841 gaatatgtaa cattaatata tatatagtca tagtatatta ttataataaa attatcttat
      901 aggtaaagga tggataaata atatgtatga ttgcactgat gaaaaattaa aactagagta
      961 tgcacgtgta tatatattac ctcaaaaata tgaaaattta tattcaatat ataaaggttt
     1021 ctggaaagga acaatattta aagatttata cagaccatat aaagaaaata aaggatgtta
     1081 atgaggtgaa actatgaaag cattagaatt attagatgat atacaaaagt tacaatttta
     1141 tgctgtggaa ttaaatcttt atttagataa tttcccggaa aatagaaagg ctactgagga
     1201 ttataaggaa atatcttcaa aattaagtga gttaatagag agatttgagt gtgaatatgg
     1261 acctataaga aattttggag atgcatatgt agaaaatcca gttgcatgga ttgaacaacc
     1321 ttggccttgg gaaattaaat attagaatat gttatttaat aagaatataa ccaaatatag
     1381 tctaatataa tcacaaaagg atgtggttat attgataaac attataaaat aaataattaa
     1441 agagttacag taatctaaaa atttgatgga tattatgtat ctatcttgtt ttaaaaaagg
     1501 gaaatattaa agtttaaaaa taagaaataa aaatacaaga gaagggagag ctactagtca
     1561 aaatattcag aagtaattag ttaaaatata aaaaaacttc ataagagatt aacttcacaa
     1621 tgttttgagt agcgtgattt aattatgtgg tattatgtta aaactttaga atatccaata
     1681 aatttaaagt gtaatgatct tagaatggca aaatttttaa tatctcagta tggtggacca
     1741 gatggagaat taagtgctgc acttagatat ttaaatcaaa gatatacaat gccaacaaat
     1801 aaatcaaaag gattattaac tgatatagga acagaagaaa tggctcacgt agaaatgata
     1861 gctactatga tttatcaatt aatggataat gcttcaattg aagatctaaa gaaagcagga
     1921 ctagctggct attatacaga tcatgggaaa gcattatact atgcagatgc acaaggcaat
     1981 ccatggacag caacttacat tcaagcaaaa ggcgatgtta tagctgattt acatgaagat
     2041 atggcagcag agcaaaaggc tagagcaact tacgaatggc ttataaactt aactgatgac
     2101 caagatataa aagaaatctt acgcttctta agagaaagag aaattgttca cttccaaaga
     2161 tttggtgaag cattaatgga cgttcaagat aaaataaaat ataaaaaata agcataaagg
     2221 tctatatcaa tatttaattt gatgtagacc tttttataat tttaatagaa attttacttg
     2281 aaatacataa aattaaacaa atttacatat tataattaaa ttgtaataaa agtaaaaata
     2341 tgtatataaa ttaaagatgt attttaaatt aaaatataga attcatctta ga
//
LOCUS       DAEVGI010000239         2383 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEVGI010000239 DAEVGI010000000
VERSION     DAEVGI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.241"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            42..689
                     /locus_tag="K8M77_002469"
     CDS             42..689
                     /locus_tag="K8M77_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7086905.1"
                     /translation="MNSFNIEKQSMWKCITKNKLICNESDTLAIILPGLGYTLDKALL
                     DYSKQLILDFKFDCLGIEYGFQVSRESFNRQDENDIRELLLETLDIIFSTLELRRGKY
                     KRIILIGKSLGTVLQGKIHKEIKENYDIHIKNIYLTPINETFKENLDKEGLIITGTKD
                     TLISSENLEKIKSEGLRVLEIEDAGHSLCIKGNVLSSIKALEIIIENIKDYLERM"
     gene            765..1208
                     /gene="msrB"
                     /locus_tag="K8M77_002470"
     CDS             765..1208
                     /gene="msrB"
                     /locus_tag="K8M77_002470"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBI7086906.1"
                     /translation="MKYKKKEKEELKKILTEEEYYVTQENGTERPFTNEYWDFNGEGI
                     YVDITTGEPLFTSKDKFHSSCGWPAFSKPIDRSIIKEKVDKSHGMVRTEVRSKLGDSH
                     LGHVFCDGPEELGGLRYCINSASLKFIPKEELKEKGYEEYLELFK"
     gene            1253..1561
                     /locus_tag="K8M77_002471"
     CDS             1253..1561
                     /locus_tag="K8M77_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086907.1"
                     /translation="MGFLLNNDGENDFLLADTYSKDDIFWITLRGLISKGKINEAEDM
                     LFKEAEKNPSMEIYEIGERMYSLLANKSEEELKKYNFSKEEIDLGLEDLKRLIDKHSL
                     "
     gene            1597..>2383
                     /locus_tag="K8M77_002472"
     CDS             1597..>2383
                     /locus_tag="K8M77_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBI7086908.1"
                     /translation="MRSLNKEVFLKKYPHVEEYIEKNKIKFEDLQRIYKDFVKFEGSY
                     YNQADFIANILRSNENVHSVKSRIKNPDRLIEKIIRKTEDRKEKYGEDFYFNEDNYKN
                     EITDLIGIRVLHIFKDQWKDIHDFIIKTWNVIEITANIRDGDDRSIFDNLGIEVISRQ
                     SGYRSVHYLVELNFTQDSTTIAEIQVRTIFEEGYGEIDHRLRYSHQEIPEILKSNLLL
                     FNRIVGSADEMASLINTLNNELVDKSAEYEKIIKDQQEEIKRLK"
BASE COUNT         1027 a          193 c          429 g          734 t
ORIGIN      
        1 tataattata taatagaatt taaaaagaag gtgattaaaa tatgaatagt tttaatatag
       61 aaaaacaatc aatgtggaaa tgcataacta agaataaatt aatttgtaat gaaagtgata
      121 ctttagcaat aatactacca ggccttgggt acaccctaga taaagcactt ttagattatt
      181 ctaaacagct tattttagat tttaaatttg attgtttagg aatagaatat ggatttcaag
      241 ttagtagaga atctttcaat agacaggatg aaaatgatat aagagaatta ttacttgaaa
      301 ctctagacat tatttttagt accttagaac ttagaagagg aaaatataag aggattattc
      361 ttataggcaa aagtttaggt acagtattac agggtaaaat acataaagaa attaaggaaa
      421 attatgatat tcatattaaa aatatttatt taactcctat aaatgagacc tttaaagaga
      481 atttagataa agaaggatta ataataactg gtactaagga tactttaata tcttcagaga
      541 atttggaaaa aataaaatct gagggattaa gagtattaga aatagaggat gctggccatt
      601 cattatgtat aaaaggaaat gtgttaagta gcattaaagc tttagaaata ataattgaaa
      661 atataaaaga ttatcttgaa agaatgtaaa tataactcat taaaataata ttttacatag
      721 gtattatttt aaaataataa taaataaaaa atggagtgat cattttgaaa tataaaaaga
      781 aggaaaaaga ggaattaaaa aaaatcttaa ctgaggaaga atattatgtt actcaagaaa
      841 atggaactga aagacctttt acaaatgaat attgggactt taatggagaa ggaatatatg
      901 tagatataac tactggagag cctttattta cttcaaagga taaatttcac tcttcttgtg
      961 gatggcctgc cttttcaaaa ccaatagata ggagcataat aaaagaaaag gtagataaaa
     1021 gtcatggaat ggttagaacg gaagttagaa gtaaactagg agattcacat ttaggccatg
     1081 tgttttgtga tggaccagag gaattaggtg gattaagata ttgcataaat tctgcttctt
     1141 taaaatttat tccgaaggaa gaactaaagg aaaagggata tgaagaatat ttagagctct
     1201 ttaaatagag ttaagtggat ataataaagg aaatagaaaa gtaggtgttt taatgggatt
     1261 cttattaaac aatgatggag aaaatgattt tcttttagct gatacttatt caaaggatga
     1321 tatcttttgg ataactttaa gaggattaat atctaaaggg aaaattaatg aggctgagga
     1381 tatgcttttt aaagaggcag agaaaaatcc ttctatggaa atatatgaaa taggagagag
     1441 gatgtatagc ttattagcta ataaaagtga agaagaactt aaaaaatata atttctctaa
     1501 agaggaaatt gatttaggtt tagaagattt aaagagatta atagataaac atagcttata
     1561 gtatatagca ataaatcaaa ataaggggag atagttatga gaagtttaaa taaagaagtt
     1621 tttcttaaga aatatcctca tgtagaagaa tatatagaga aaaataaaat taaatttgaa
     1681 gatttacaaa ggatatataa ggattttgta aagtttgagg gttcttatta taatcaagct
     1741 gattttatag ccaatatact tcgttctaat gaaaatgttc attctgttaa atcaaggata
     1801 aaaaatccag atagattaat agaaaaaata attagaaaaa cagaagatag aaaagaaaag
     1861 tatggtgaag atttttattt taatgaggat aactataaaa atgaaataac tgacttaata
     1921 ggaataaggg ttttacatat atttaaggat caatggaaag atatacatga ttttataata
     1981 aagacttgga atgtaattga aataacagca aatataagag atggagatga tagaagtatt
     2041 tttgataatc taggtattga agttatatca agacaatcag gatatagatc agttcattat
     2101 ttagttgaac taaattttac ccaagatagt acaacaatag ctgagataca agtaaggact
     2161 atttttgagg aggggtatgg agaaattgat catagactaa gatattccca tcaagagata
     2221 ccagagatct taaaatctaa tcttttactt tttaatagaa ttgtaggaag tgcagatgaa
     2281 atggcttcat tgataaatac attaaataat gaattagttg acaaaagtgc tgaatatgaa
     2341 aagataatta aagatcagca agaagaaata aaaagactaa aac
//
LOCUS       DAEVGI010000240         2299 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEVGI010000240 DAEVGI010000000
VERSION     DAEVGI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.242"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            271..1710
                     /locus_tag="K8M77_002473"
     CDS             271..1710
                     /locus_tag="K8M77_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBI7086909.1"
                     /translation="MMKYFQKLGKSLMLPVAALPVAGILMGIGYWLDPSGWGANSVVS
                     AFLLKSGGAIIDNMAILFAIGVAIGMSDDNDGAAGLAGLVSWLMITTLLSSAVVAMMQ
                     GVEVEAVNPAFGKIQNQFIGIVAGLIGAGCYNRFKNTKLPDFLGFFSGRRCVAIVTAF
                     ASIIASLVLYFVYPVIYSALVSFGESIIAMGPIGAGVYGFFNRLLIPLGLHHALNSVF
                     WFDVAGINDIGNFWAGTGTLGQTGMYMSGFFPVMMFGLPAAAFAMYRCAKTNKKKAAA
                     GILLAAALSSFVTGVTEPLEFAFMFLAPALYVAHALLTGISMAVVAALPVRAGFNFSA
                     GLVDWILSFASPMALNPLYLLGIGLVVGVIYYVVFVVMIKKFDLKTPGREEDDDSNAV
                     LANNNYTEVAKIILEGLGGPSNITSIDNCITRLRLQVKDNTLVDEKKIKSSGISGIIR
                     PGKTSVQVIVGTQVQFVADEFKKLCKSTN"
     gene            1927..>2299
                     /locus_tag="K8M77_002474"
     CDS             1927..>2299
                     /locus_tag="K8M77_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086910.1"
                     /translation="MFRLNLKEEDIIMKIAVMKKVSNKDVAINFISNLVTRAGGDVAY
                     KINLIVTKDSLYLEHVGHASLGYAEETREVDHIPFKDLKEFSVTTKGENEEVKILTSN
                     KEFNLFKDNSKGDNLASTMGKV"
BASE COUNT          810 a          257 c          431 g          801 t
ORIGIN      
        1 catcaatcat ggggaagtag ttatttaaaa ttattaagtt taatttttac taaattattt
       61 taggaggtgt tataaataaa aaataatatt atgtaagcta taaaattcat aattggtttt
      121 atagcttaag aaaacgtttt aaagcaatca taaataaata tactctaatt tattaattta
      181 ctatggaaga ttagtttgat tctttcagaa aaaaggattt ttaaagaatc aacttaacag
      241 atcggtagaa tttatagggg gaatattaat atgatgaaat attttcagaa attaggtaaa
      301 tctttaatgc taccagttgc tgcgcttcca gtagcaggta tattaatggg aattggatat
      361 tggttagacc catctggatg gggtgctaat agtgtagtat cagcattctt attaaaatct
      421 ggtggagcta tcatagataa catggcaata ttatttgcta ttggggtagc tattggtatg
      481 tcagatgata atgatggagc agcaggttta gcaggtctag tatcatggct tatgataact
      541 acattattat ctagtgcagt tgttgcaatg atgcaaggag tagaagtaga agctgtaaac
      601 ccagcttttg gaaagataca aaaccaattt ataggtatcg tagcaggttt aataggagct
      661 ggttgttata atagatttaa aaacactaaa ttaccagatt tcttaggatt ctttagtgga
      721 agaagatgtg ttgctatagt aacagcattt gcttcaataa tagcctcttt agttttatac
      781 tttgtatatc ctgtaatata tagcgcatta gtttcatttg gtgaatcaat aatagctatg
      841 ggaccaatag gagctggtgt ttatggattc ttcaatagat tattaatacc tcttggttta
      901 caccatgctt taaactcagt attctggttt gacgttgctg gtataaatga tataggaaac
      961 ttctgggctg gaacaggtac tttaggacaa actggtatgt atatgtcagg tttcttccca
     1021 gtaatgatgt ttggtttacc tgctgctgct tttgcaatgt atcgttgtgc aaaaactaat
     1081 aaaaagaagg ctgctgctgg tatattatta gctgctgctt tatcatcatt cgttacagga
     1141 gtaactgaac cattagaatt tgcatttatg ttcttagcac ctgctttata tgtagctcat
     1201 gctttattaa caggaatatc tatggcagtt gttgctgctt taccagtaag agcaggattt
     1261 aactttagtg ctggtttagt agactggata ttaagttttg catcaccaat ggcattaaac
     1321 ccattatact tattaggcat aggtttagta gtgggagtta tatactatgt agtattcgta
     1381 gttatgataa agaaatttga cttaaagact ccaggtagag aagaagatga tgattcaaat
     1441 gcagttttag caaataataa ctacactgaa gttgcaaaaa tcatcttaga aggactaggt
     1501 ggaccttcta acattacttc aattgataac tgtataacta gattaagatt acaagtaaaa
     1561 gataatacat tagttgatga gaaaaaaata aaatcatcag gtatttcagg aattataaga
     1621 cctggaaaaa caagtgttca agttatagtt ggaacacaag tacaatttgt agcagatgaa
     1681 tttaagaagc tttgtaagtc tacaaattaa gtatagttaa taggttttta aagttttaat
     1741 ataaaatctt ttatactaaa aatttaatag ttatattaaa gcttttaaat aaaaaataat
     1801 aaaatatccc ttatattaaa aatccagtag agtatttata tagctctact ggcattctta
     1861 gttataaatt ttgttatata caattaggaa gatattgatt gtttgtaata ttaatgaggt
     1921 gtttttgtgt ttagattaaa tttaaaagaa gaagatatta taatgaagat tgcagttatg
     1981 aaaaaagtta gtaataagga tgtagctata aattttatat caaatttggt aactagagct
     2041 gggggagatg tggcgtataa aataaatcta attgtaacta aagattctct ttatttagaa
     2101 catgtaggac atgcatcctt aggttatgct gaagaaacaa gagaggttga tcatatacca
     2161 tttaaagatt taaaagaatt ttcagtaact actaaaggtg aaaatgagga agtgaaaata
     2221 ttaacttcta ataaagaatt taatcttttt aaggataatt caaaaggtga taacttagct
     2281 tcaactatgg gtaaggtag
//
LOCUS       DAEVGI010000241         2260 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEVGI010000241 DAEVGI010000000
VERSION     DAEVGI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.243"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(487..945)
                     /locus_tag="K8M77_002475"
     CDS             complement(487..945)
                     /locus_tag="K8M77_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="HBI7086911.1"
                     /translation="MKLAEALILRADLQKELMSLKNRINQNLLVQEGEKPSENPYTLY
                     DEILLKVSELEGLILKINKTNSNYITSNNESISDLIVKRDMLGKKISILKEIAEEATV
                     KVNRYSQTEIKIFSAMKVDQLQREINKYSKEYRELDTKLQGLNWTIDLME"
     gene            complement(1137..2168)
                     /locus_tag="K8M77_002476"
     CDS             complement(1137..2168)
                     /locus_tag="K8M77_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBI7086912.1"
                     /translation="MQVLIGAGLLLIVLGLFTLFSYKAPHGMKAMGALAGAACASFLV
                     EAFHSSFFGNVLNIEFLKKVGDANGSLGGVAAAILVPLALGVSPVYSVLIGLSCSGLG
                     ILPGFIAGYLMSFVVKFLEEKLPSGLDLIVIIVVATPLTRIIGDFTAPVVDSTLLKIG
                     QVLIASANSNPITMGIILGGIITVVATAPLSSMALTAMLGLTGAPMAIGALSVFGSSF
                     LNFVLFQKMKFGSKKDTISVAIEPLTQADIITANPIPIYVTNFIGGALCGIIIVLMGL
                     TNMSPGTATPIAGFAVMFAYNPAFKVLMASLACVVVSAGSGYLGYLLFRNYKIHTVDE
                     IRGNDLEVE"
BASE COUNT          776 a          392 c          242 g          850 t
ORIGIN      
        1 tatttgctat aactaaagaa gaaaattctt taaaaagtat ttttctacat ttaattttag
       61 aaattattag taagctaaaa ctagttaaaa ttaaattttt agaatagaat tatataaaaa
      121 gatatgtatt aactttaatt agctttattt actttaaact ttgtaattta aagtaaaaaa
      181 atagagctat aatttcctta atgaatcata actctatctt ctttaaattg tatcccctta
      241 ccccatccta gcttattatt aatagtcata tggttgtaaa ttgtaattct taaatagtac
      301 ccatgtaatt tgtttgagca taaattataa aataagtatc tgctcctaga tacatgtata
      361 atcacctttt tccccaggtt aggatttatg acctccttaa taaaagctac caacatttaa
      421 acctctattt aattaatcta ttatctagtt taaactagga aaattaactt tataatttag
      481 atacttttat tccattaaat ctattgtcca gtttaaacct tgcaacttag tgtctagttc
      541 tctatattcc ttagaatatt tatttatttc tctctgtaat tgatctacct tcattgcaga
      601 aaatattttt atttcagttt gagagtatct atttactttt actgtagctt cttcagctat
      661 ttcttttaat atgcttatct tctttccaag catatctctt tttacaatca aatcagaaat
      721 actttcatta ttactagtta tgtaattact attagtttta ttaatcttta gaattaaccc
      781 ctctaattca cttactttta acagtatctc atcatatagt gtatatgggt tttcagaggg
      841 tttctcacct tcttgaacta gtaagttttg atttattcta ttttttaaac tcattaattc
      901 tttttgtaaa tctgctctta aaatcaatgc ctcagctaat ttcattttaa tcactccaat
      961 aaaaatttta ttaaactcgg cttcataaac ctttttaatt catatgaacc taatacatgt
     1021 agaccaaatt tattagttag taatattcaa gataattcta atatttattc ttattataca
     1081 actaaaaatt attaagtata tataaaagat tcatttatta taaaaataaa atttaattat
     1141 tctacctcta aatcatttcc acgtatctca tctactgtat gtattttgta atttctaaat
     1201 aataaatacc ctaggtatcc actacctgca cttacaacta cacaagctag agaagccatt
     1261 aataccttaa atgctgggtt atatgcaaac attacagcaa aaccagcaat aggggtagct
     1321 gttcctggag acatattagt tagtcccatt aaaactatta taatcccaca taaagcgcca
     1381 ccaataaaat tagttacata aataggaata gggttagctg ttattatatc cgcctgcgtc
     1441 aatggttcta tggcaactga tattgtatct tttttgcttc caaacttcat tttttggaat
     1501 aaaacaaaat ttaagaaaga tgagccaaaa actgataatg ctcctattgc cattggtgct
     1561 cctgttagtc caagcatagc tgttaaggcc attgaactta aaggtgccgt agcaactaca
     1621 gttataattc ctccaagtat tattcccatt gttattggat tagagtttgc tgaagctatt
     1681 aaaacctgtc ctatttttaa taaagtagaa tcaactactg gtgctgtaaa atcacctatt
     1741 attcttgtaa gaggcgtagc cacaactatt attacaatta aatctaaacc acttggtaat
     1801 ttttcttcta agaatttaac cacaaatgac attaaataac ctgctataaa tcctggtaat
     1861 attcctaatc ctgaacatga tagtcctatt aaaactgaat aaactggtga aactcctaaa
     1921 gctaatggta caagaattgc tgctgccact cctcccaagc ttccattagc atctccaacc
     1981 ttctttaaaa actctatatt tagaacattt ccaaagaatg atgagtgaaa tgcctcaact
     2041 aaaaagcttg cacaagctgc tcctgccaat gctcccatag ccttcattcc atgtggtgcc
     2101 ttataactaa ataaggtaaa caatcctaaa actattaata aaagtcctgc cccaattaaa
     2161 acttgcataa attttcctcc ttagttttta aattttaaac ttaccttaat tatttacttt
     2221 tcatatataa taatattaat ttataaacat tcttaaaagt
//
LOCUS       DAEVGI010000242         2247 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEVGI010000242 DAEVGI010000000
VERSION     DAEVGI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.244"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            154..1380
                     /locus_tag="K8M77_002477"
     CDS             154..1380
                     /locus_tag="K8M77_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBI7086913.1"
                     /translation="MKPEELLKEANLLQETIVSNRRYLHSHAETGFDLKNTLAFVKKE
                     LIDMGYEPIECGKAGLIALAGGKKEGKVFLIRGDMDALPIKEESDVEFSCQSGKMHAC
                     GHDMHTSMMLGAARLLKKHEDEIEGTVKLMFQPAEEIFEGSKDMIKAGVLENPKVDQA
                     LMIHVMAGMPFNAGTVIVPVPGIGAPAADYFEIKVQGKGCHGSMPNTGVDPLNVAAHI
                     LIALQEIHARELAISDQAILTIGTMNAGIAANVIPDTATMGGTIRTFDEETRSFIKER
                     IEEIAECTAKSFRASAEVIWGSGCPTLVNDKDLTVCSEKYIKELLGEDKTFSVAKLNA
                     MAGNQKAAKTSGSEDFAYISQKVPAIMLVLAAGHPDKGYPYPQHHPMVKFDEEVLSSG
                     SAVYAYTAMRWLQDHK"
     gene            1449..2168
                     /locus_tag="K8M77_002478"
     CDS             1449..2168
                     /locus_tag="K8M77_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086914.1"
                     /translation="MKKNKIIIIPTLAILIIIFCITIYNIKNPVIKGLYGNMEIINYN
                     GQTAEMDSFFSDSTEAEYLGRTEDKNFEVYAYKNGSNYNLFTLFGSDNTNTYKAPNFS
                     IPKDGEVTKVFLDPNTREINNKVIKNQSDIEMFKKLVSYKNSEDVYHINNIYTEGTEI
                     YFAYDNCPVATSDNLVGYITYIDHNWILVSPENYYGSSNNTLYSNEADLIGSKITDSK
                     LIKWLNDNETEVAPPSYKEKL"
BASE COUNT          867 a          329 c          367 g          684 t
ORIGIN      
        1 ataaaaatct tattttttaa aatcttatct ttaataacaa ttaaactttt tgaaaaataa
       61 ttattaaatc ataactttta aaaatacttt agatttatga atatgataaa ataataatat
      121 tactcaaaaa aagaactttg aaaggagaac ataatgaaac cagaagaatt actaaaagaa
      181 gctaatttac ttcaagaaac tattgtttca aacagaagat atttacactc acatgctgag
      241 acaggatttg atttaaaaaa cactcttgcc tttgtaaaaa aagagttaat tgatatgggc
      301 tatgagccaa tagaatgtgg aaaagcagga cttattgccc tagcaggtgg aaaaaaggaa
      361 ggaaaggttt tcttaataag aggtgacatg gatgccttac caattaaaga ggaatcagat
      421 gttgagtttt cttgccaaag tggaaaaatg catgcctgtg gccatgatat gcacacatct
      481 atgatgcttg gtgctgcaag attattaaaa aaacatgagg atgaaattga aggaactgtt
      541 aaacttatgt tccaacctgc tgaggaaatt tttgaaggtt ctaaggatat gattaaagca
      601 ggagtattag aaaatccaaa ggttgatcaa gccctaatga ttcatgtaat ggcaggaatg
      661 ccttttaatg caggtactgt tattgttcct gtacctggaa ttggagctcc agctgctgat
      721 tattttgaaa taaaggttca aggaaaagga tgtcatggtt ctatgcctaa cactggagtt
      781 gacccactta atgtagctgc tcacatttta attgcattac aagaaattca tgctagagaa
      841 cttgccataa gcgatcaagc aattttaaca attggaacaa tgaatgctgg tattgctgca
      901 aatgtaatcc ctgacacagc tactatggga ggaactattc gtacctttga tgaagaaaca
      961 cgctcattta taaaggaaag aattgaagaa attgcagaat gtactgcaaa atcctttaga
     1021 gcttcagctg aagtaatctg gggaagtgga tgcccaacct tagttaacga taaagactta
     1081 actgtatgct ctgaaaaata cataaaagaa cttttagggg aagataaaac attctctgtt
     1141 gccaaactaa atgccatggc aggaaaccaa aaagctgcta aaacttctgg ttcagaagac
     1201 tttgcttaca taagccaaaa agttcctgct atcatgttag tcttagctgc tggccaccca
     1261 gataaaggat atccataccc acagcaccac ccaatggtta aattcgacga agaagtttta
     1321 tctagtggta gtgcagttta cgcctacaca gcaatgcgtt ggcttcaaga ccataagtaa
     1381 ttaagaaaat tctaattttt aaactatata taataaaaac aacctaaaat tataggggga
     1441 aaattcttgt gaaaaagaat aaaataataa ttatacctac tttagctata ctaatcataa
     1501 ttttctgtat tactatttat aatataaaaa atccagtaat caaaggttta tatggcaata
     1561 tggaaatcat taactacaat gggcaaactg cagaaatgga tagctttttt tctgactcta
     1621 ctgaagcaga atatttaggg cgtacagaag ataaaaattt tgaagtctat gcatataaaa
     1681 atggttctaa ttataactta tttactcttt ttggttctga taatactaat acatataaag
     1741 ctccaaactt ctcaatcccc aaagatggcg aagttactaa agtattcctt gatccaaata
     1801 ctagagaaat taataataaa gttataaaaa atcaatctga tatagaaatg tttaagaaac
     1861 ttgtttcata taaaaacagt gaagatgtat atcacataaa taatatatac acagaaggta
     1921 ctgaaattta ttttgcatat gataattgtc ctgttgctac ctctgacaat ttagttggat
     1981 acataacata tattgaccat aattggattc ttgtaagccc agaaaattat tatggtagtt
     2041 ctaataatac actatattca aatgaagcag atttaattgg aagcaaaata acagattcta
     2101 aactaattaa atggcttaat gacaatgaaa ctgaagtagc acccccatct tataaagaaa
     2161 agctttaatt taattttcaa ataatatttt aaagcttaaa ctaaaattca atttttatta
     2221 aaaaactatg atattaatat aattaaa
//
LOCUS       DAEVGI010000243         2243 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEVGI010000243 DAEVGI010000000
VERSION     DAEVGI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.245"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..271)
                     /locus_tag="K8M77_002479"
     CDS             complement(<1..271)
                     /locus_tag="K8M77_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086915.1"
                     /translation="MLKVFFSFKKHFIEHKLLILSVVLSLFMSSILKWSFFKYIVGIL
                     SFCMIMLDYKESLGNFVESKKLIYKKYINNISFLVDSLILGFIINE"
     gene            579..2027
                     /locus_tag="K8M77_002480"
     CDS             579..2027
                     /locus_tag="K8M77_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI7086916.1"
                     /translation="MNNANENSKNTENLVYKNRWFILAVLVMQPFMACLDSSIVNVAL
                     PILQKELGATMAGTEWIVSSYLIIISASILIFGRLGDMNSKSSVFIRGILIFVLGSFL
                     CGISPSLPILVLSRVVQAIGAAMLMSTNQGIITDVFPPKERGKALGISGSFVALGSIL
                     GPALGGFIVTYFTWHYIFLINVPIGIICYIFAKKYLPIRIPKESESMDKPGATMFFIA
                     TILIFTSILISQHLGFTSIYVILGLIIGIIVLAFFLIYENKIKNPMLHLSLFKKPIFS
                     VSLICAFISFTAINTINVIQPFYLNDILGYSPGKISVIMTAYPIVLFVIAPLSGTLSD
                     KIGSKTLTLIGLFLTGVSMILLSSLNSNSSETSIFLMLSLLGFSNGLFQSPNTNLIMS
                     SVPKDKLGIAGGTNALIRNLGLAFGVSASTSILYAKMSKLAGFRVLGSVPNRPDIFIS
                     GMRFVYILVAISCFICLIISVIEYIQSKKNKK"
BASE COUNT          791 a          305 c          289 g          858 t
ORIGIN      
        1 cctcatttat tataaatcct aaaattaaac tatcaactaa aaaggatata ttatttatat
       61 actttttata tattaatttt ttgctttcaa caaaattacc taaactctct ttatagtcta
      121 gcataatcat acaaaaagat aatataccaa ctatatattt gaagaaactc cattttaata
      181 tgctagacat aaatagtgaa agaaccactg aaagaatcaa tagcttatgt tctataaaat
      241 gttttttaaa agaaaaaaat accttaagca tattaagtcc tccttgatgt aacttatttc
      301 ctttgtatct attctaacac ttttatatga ataatgacat ataatatttt cctcttcaaa
      361 attaaaataa tatttttagg taagcttatt ttaattaaag gcaactttta ataaaggaag
      421 taattattag aaatttagta ttttttaatt aataaaagtc ctctatacat ggaaaataag
      481 cgttttttca attttgacaa taaaattttg tcgttttata attgtatagt taatgtttaa
      541 caaaaatttc attaaaattt aataaaggag atgattaagt gaataatgct aatgaaaact
      601 ctaagaatac ggaaaattta gtttataaaa acagatggtt tatattagca gtattagtaa
      661 tgcaaccatt catggcatgt ttagatagta gcatagttaa tgtagcactt ccaattcttc
      721 aaaaagaact tggtgcaact atggctggga ctgaatggat tgtaagttca tatttaataa
      781 taatttctgc atcaatttta atctttggta gattaggaga tatgaattca aaaagttcag
      841 tatttataag agggatttta atatttgttc ttggatcttt cctttgcgga atatccccat
      901 cgcttcctat tttagtatta tctagagttg tgcaagctat tggtgccgct atgttaatgt
      961 caactaatca aggtattata acagatgttt tccctcctaa ggaaagagga aaagctttag
     1021 gtatctctgg ttcctttgtt gccttaggtt ctatattagg tccagcatta ggtggattca
     1081 tagttacata tttcacttgg cactatattt tcttaataaa tgtacctata ggtataattt
     1141 gctacatatt cgctaaaaaa tatctaccaa taagaattcc taaagaatct gagtcaatgg
     1201 ataaacctgg agcaacaatg tttttcattg ctacaattct tatatttaca tcaatattaa
     1261 ttagccaaca tttaggcttt actagcatat atgtaatcct aggattaata attggaatta
     1321 ttgttcttgc tttcttctta atctatgaaa ataagataaa aaatcctatg cttcatttaa
     1381 gtttatttaa aaaaccaata ttttcagtaa gtttaatatg tgcatttatt tcctttactg
     1441 caataaatac aattaatgtt attcaaccct tttacttaaa tgatatttta ggttatagtc
     1501 ctggaaaaat ttcagttata atgacagctt atccaattgt attatttgtt attgcaccac
     1561 ttagtgggac attatcagat aaaataggaa gtaaaacact tactttaata ggattattct
     1621 taacaggagt ttcaatgatt ttattatcat cattaaatag taactcaagt gaaacatcta
     1681 tattccttat gttatcctta ttaggttttt caaatggact attccaatct ccgaatacaa
     1741 accttataat gtcctctgtt cctaaggata agcttggaat agcaggtggt actaatgcct
     1801 taataagaaa tttaggtctt gcctttggag tatcagcttc aacctctatt ttatatgcaa
     1861 aaatgagtaa attagctggt tttagagttt taggatctgt tcccaataga cctgatatat
     1921 ttatttctgg aatgagattt gtatatattt tagttgctat ttcatgtttt atatgcctta
     1981 taatttctgt aattgaatat attcaatcta aaaaaaataa aaaataaaaa aataaaaggt
     2041 gctgtatcaa agattttatt aaaatcttat ttgatacagc acctttttaa ttattagttt
     2101 cttgtatgct cattattcct tgaggattgt taccaacctc taatcttgct aaattgtcag
     2161 aattgctaaa ataaatattt cctcccgcaa atactaaaag tgctaaaatt atcactaata
     2221 aaatattttt gcttctaaaa ttc
//
LOCUS       DAEVGI010000244         2231 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEVGI010000244 DAEVGI010000000
VERSION     DAEVGI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.246"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..188)
                     /locus_tag="K8M77_002481"
     CDS             complement(<1..188)
                     /locus_tag="K8M77_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxychorismate lyase"
                     /protein_id="HBI7086917.1"
                     /translation="MIYIGENKKGYSMFEKCLLTIDSSVFFGRGIFETILVLNNGVFL
                     KEHLERLKDGCLNLNIPF"
     gene            complement(206..1552)
                     /gene="pabB"
                     /locus_tag="K8M77_002482"
     CDS             complement(206..1552)
                     /gene="pabB"
                     /locus_tag="K8M77_002482"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBI7086918.1"
                     /translation="MNFEIKEIENKLKPHEVYFLFKDEINSIFLDSSKEDSEFSLYSF
                     IGLNPFKKFISKGNEIFIDEKLVLGDPFKKLEELILEYKINYNSEIPFLGGAMGYFSY
                     DIGRVIEDMPKSAKDDVCIPDSYFLFYDNVIIFDLRNQKTYITALGIKEESEKSIANI
                     YERIKDKDINLNISLDKNTEFTSNFSREEYINSVKKVKEYIKEGHTYIANLTQRFSCD
                     FNSDPFDTYISLRSINKAPFSCYLNLEGFQIISSSPERFLKVFNNKVETRPIKGTRPR
                     GKDKLEDEKNKKELLNSEKDKSELLMIVDLERNDLSKVCKPFSVKVTELFKLEEYATV
                     FHLVSTVIGELKEDVSSVKCIRECFPGGSITGAPKIRSMEIIEELEGLRRGIYTGAIG
                     YFDLRGNCDFNIVIRTILAKDNKAYFGVGGGITIESEEEMEYEETLDKAKALMRVL"
     gene            complement(1558..2148)
                     /locus_tag="K8M77_002483"
     CDS             complement(1558..2148)
                     /locus_tag="K8M77_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBI7086919.1"
                     /translation="MFLMIDNYDSFVFNLLRYFKELNEDIKIKRNDKITIEEIKRLNP
                     EGIIISPGPKAPKDMKECLNIIEKFKGKIPILGVCLGHQCIGYSFGSKIKKGDFPVHG
                     KVSKITHDGKGIFKGIKNNINVTRYHSLVVDRNSLGEDLEITALSDDGVVMGIRHKRY
                     NIEGVQFHPEAVLTEYGHEMLKNFIDNARKFNIIER"
BASE COUNT          735 a          424 c          186 g          886 t
ORIGIN      
        1 ttaaaaggaa tatttaaatt taaacatcca tcttttagtc tttctaaatg ttcctttaaa
       61 aacactccat tatttaatac aagtatagtt tcaaaaattc cccgtccaaa aaaaacagaa
      121 gaatctatgg taagtaaaca tttttcaaac atgctataac cttttttatt ttctcctata
      181 taaatcactt tatatcacaa cctaattata atactctcat taaagccttt gccttatcta
      241 aagtttcctc atattccatc tcttcttctg attcaatagt tatgcctcca cctactccaa
      301 aataagcctt attatctttt gctaatatag ttcttattac tatattaaaa tcacaatttc
      361 ctcttaaatc aaaataacct atggcaccag tatatatccc tcttctaagg ccttctagtt
      421 cctctattat ctccatactt cttattttag gagcgcctgt tatagaacct cctggaaaac
      481 attctcttat acattttaca gaactaacat cctcctttag ttctcctata actgtggaaa
      541 ctaagtgaaa tactgtagca tattcctcta atttaaataa ttctgtaacc ttcacagaaa
      601 aaggtttgca taccttactt aagtcatttc tctctaaatc aacaatcatt aaaagctctg
      661 atttatcctt ttcactattt aaaagttctt ttttattctt ttcatcttct aatttatctt
      721 ttcctcttgg tcttgtgcct ttaattggtc ttgtttcaac cttattatta aacactttta
      781 aaaacctttc aggagaagaa cttataattt gaaaaccttc taaatttaaa taacaggaaa
      841 aaggggcttt atttatactt cttaaactta tatatgtatc gaaaggatca gaattaaaat
      901 cacaagaaaa tctctgagtt agatttgcaa tatatgtatg cccttctttt atgtattctt
      961 taaccttttt taccgaattt atgtattctt ctcttgaaaa atttgaagta aactcagtat
     1021 ttttatctaa gctgatattt aaatttatat ccttatcttt tatcctctca taaatattag
     1081 ctatactttt ttctgactct tccttaattc ctagggctgt tatatatgtt ttttgatttc
     1141 tcaaatcaaa tattattaca ttatcataaa ataaaaaata actatctgga atacacacat
     1201 catccttagc acttttaggc atatcttcaa taactcttcc tatatcataa gaaaaatatc
     1261 ccatagcacc tcctaaaaaa ggtatctcac tattataatt tattttatat tctaaaataa
     1321 gctcttctaa ttttttaaaa ggatctccta agactagttt ttcatctata aaaatctcat
     1381 ttcccttaga gataaacttt ttaaaaggat ttaatcctat gaatgaatat aaagaaaatt
     1441 cgctatcttc ctttgaacta tctaaaaata tactgtttat ttcatcttta aataaaaaat
     1501 atacttcatg tggcttaagc ttattttcta tttccttaat ttcaaaattc aaattactca
     1561 cctttctata atattaaatt tcctagcatt atcaataaaa tttttaagca tttcatggcc
     1621 atattcagtt aaaacagcct ctggatgaaa ctgtactcct tctatattgt accttttatg
     1681 ccttattccc ataactaccc catcatcact taaggcagtt atctctaaat cttctcctaa
     1741 agaatttcta tcaactacta aagaatgata tctagttaca tttatattat tttttattcc
     1801 cttaaaaatc ccttttccgt catgagttat tttgctcact tttccgtgga ctgggaaatc
     1861 cccttttttt attttacttc caaaggaata tccaatacat tggtggccaa gacatacacc
     1921 taaaatagga atcttcccct taaatttctc tataatattt aaacattctt tcatatcctt
     1981 aggtgcttta ggtcctggag atattattat tccctctgga ttcaatcttt ttatttcttc
     2041 tatagttatt ttatcattcc tctttatctt tatatcctca tttaattcct taaaatacct
     2101 aagcaaatta aacacgaagg aatcgtagtt atctatcatt aaaaacatta attcacctct
     2161 aaaatattta gtttaaaatc attatatcaa aaacacctaa aaaaaaatat gaattttata
     2221 ccatttatct a
//
LOCUS       DAEVGI010000245         2207 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEVGI010000245 DAEVGI010000000
VERSION     DAEVGI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.247"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(23..733)
                     /locus_tag="K8M77_002484"
     CDS             complement(23..733)
                     /locus_tag="K8M77_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7086920.1"
                     /translation="MLSIALCEDNSFQREELKNNLSKVLDEIGVEYKLLTFEIGEDLL
                     REYPEKLDVLFLDIQMGELTGMDTARKVREYDDKVEIIFITALWDYIQKGYEVRAFRY
                     LIKPVKFKELQEQVTACVENILYKRYTYITIKDKNNVLKIRTEDIIFLETFERKVIIH
                     TNSQDYIVKMSMNKLEKELNNKGFFRCHTSYIVNLIKVEEVKKDYLLINKFTLPVSKH
                     RMKNLKLRLTSLLGDLIC"
     gene            complement(872..1888)
                     /locus_tag="K8M77_002485"
     CDS             complement(872..1888)
                     /locus_tag="K8M77_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7086921.1"
                     /translation="MKIAVRGGHNFQAKGSSALIDETIENRKVYKALIKYLSIAGHNV
                     IDVTPGECDVNTDLYLGVQKAEDNNVELFISIHFDKAYDRYEGALGTGTWIYGRGGKA
                     EIYAKRIVDNLSKGTGLKNRGVKTNPKLYELRKTSMPAILVEVCFCEATEDVRIYKEK
                     GTDLIGKLIAEAINGDEIVGGDTRPENPEGSLKEKFLKSTNAKAIANLDPRDNPSSIY
                     KDLGEIYKGEKIRVLPEVCDKKDYLPIIYWKDTPNTESQRVWVNANQNYLKIDTNATV
                     INVVTELDARYTKSKTSSKMGYVKNGERLYIHKIENGYALGTYFASNGYKTAWFTAKY
                     ISLD"
     gene            complement(1984..2207)
                     /locus_tag="K8M77_002486"
                     /pseudo
     CDS             complement(1984..2207)
                     /locus_tag="K8M77_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455890.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
BASE COUNT          686 a          430 c          188 g          903 t
ORIGIN      
        1 aaatcccaaa aaatttgatt cactagcata ttaaatctcc taaaaggcta gttagcctta
       61 attttaaatt tttcattcta tgcttactta caggcaaagt aaatttattt attaaaagat
      121 aatccttttt aacctcttct acttttatta gatttactat ataactagtg tgacatctaa
      181 aaaaaccctt attattaagt tccttttcta atttgttcat actcatcttt actatataat
      241 cttgagaatt agtatgtatt attacctttc tctcaaaagt ttcaagaaaa attatatctt
      301 ctgttctaat ctttaaaaca ttatttttat cttttattgt tatgtatgta tatcttttat
      361 ataaaatatt ttctacacaa gctgtaactt gctcttgtaa ctccttaaat ttaactggct
      421 ttattaaata tctaaaagca cgaacttcat aacctttttg aatataatcc catagagcag
      481 ttataaatat aatctctact ttatcatcat actctctaac ctttctagca gtatccatac
      541 ctgttaattc acccatttga atatctaaaa aaagcacgtc taatttttct ggatattctc
      601 ttaatagatc ctctcctatc tcaaaggtta aaagtttata ctctactcct atttcatcta
      661 aaactttact taaattattt ttcaattctt ctctttgaaa ggaattatct tcacataaag
      721 caatactaag catgtctaat aatctccttt ttaacttata tttcttatgt aattttatca
      781 tattttattt tatattgctc aagtttataa aacaaatttt aaatattaaa atctaattaa
      841 acaagaccta gaaaaccagg tcttataaaa atcaatctaa acttatatat tttgctgtaa
      901 accaggctgt cttatatcca ttacttgcaa aataagttcc tagtgcatat ccattttcta
      961 ttttatgaat ataaagcctt tcaccatttt tcacataacc catcttactg gatgttttag
     1021 attttgtata cctagcatct aattctgtaa ctacatttat aacagtggca ttagtatcta
     1081 tttttaaata attctgattt gcattaaccc ataccctttg agattctgta ttaggtgtat
     1141 ctttccaata gattataggc aaataatcct ttttgtcaca tacctctggt aaaactctta
     1201 ttttctcacc tttatatatc tctcctaaat ctttatatat gctacttgga ttgtctctag
     1261 ggtctaaatt agctatagct tttgcattag ttgattttaa aaatttctct tttaaagaac
     1321 cttccggatt ttcgggtctt gtatctcctc ctactatttc atcgccattt atagcctcag
     1381 caattaactt tcctataaga tctgtgcctt tttctttgta tatcctaaca tcctcagttg
     1441 cttcacaaaa acaaacctcc accaaaatag ctggcattga tgtttttctt aattcatata
     1501 gtttaggatt tgtcttaaca cctctatttt taagtccagt tccctttgaa agattatcta
     1561 ctattctctt agcatatatc tctgcttttc ctcctctgcc atagatccaa gttccagtac
     1621 ctaaagcccc ttcatatcta tcataagctt tatcaaagtg tattgatata aataattcaa
     1681 cattattatc ttcagccttt tgaactccta gatataaatc agtattaaca tcacactctc
     1741 ctggggttac atctataaca ttatgacctg ctatgcttaa atattttatt aaagctttat
     1801 aaacctttct attttcaata gtttcatcta ttaaagcact agaacctttt gcctgaaaat
     1861 tatgtccccc tcttactgct attttcataa aaatacctcc taaaaatatc ttctttcact
     1921 atataaatat tttaaagagg caacttaagt tacattattc aaaaatttat ttttaacttt
     1981 atattatcta tactttccaa ccttttcatc cattgttact ccgccttttt ataatcaatt
     2041 tttactattg atataactct tccagcaccc tcttcaatac atatttcttg agattttttc
     2101 acaatttcta gtaactcatc aatttttcct tccattgtag tttccatagg acctacctca
     2161 aactttactc ctgttgattc tatatattca ataaccttat caacaac
//
LOCUS       DAEVGI010000246         2158 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEVGI010000246 DAEVGI010000000
VERSION     DAEVGI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.248"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(68..1081)
                     /gene="aroF"
                     /locus_tag="K8M77_002487"
     CDS             complement(68..1081)
                     /gene="aroF"
                     /locus_tag="K8M77_002487"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBI7086922.1"
                     /translation="MIVILKPGTKEEEILKFIKKIESLGVETQRISGSEMCVIGLVGD
                     TSKIDPAKVEANKNVERIMPVQEPFKKANRLFHPENSIIDVLGNKIGDKKIALIAGPC
                     SVESEEQITEIAKEVKALGASFLRGGAFKPRTSPYSFQGLELEGLELLKKAKAKTGLP
                     IVTEIMSTSMIEKFIEDVDVIQVGARNMQNFDLLKELGKTNKPILLKRGLSATIEELI
                     MSAEYIMSGGNENVILCERGIRTFETYTRNTLDLSAIPAIKKLSHLPVIVDPSHAAGK
                     SWMVEPLSKAAIAVGADGLIIEVHNDPANALCDGQQSIKPEEYGKLLEDLRAIAKAVG
                     REL"
     gene            complement(1553..1828)
                     /locus_tag="K8M77_002488"
                     /pseudo
     CDS             complement(1553..1828)
                     /locus_tag="K8M77_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
BASE COUNT          677 a          417 c          240 g          824 t
ORIGIN      
        1 acgggtgttc ttgccaaagg cataaattta atgggagttt aaaaccccca ctaaattaaa
       61 taatattcta taattctcta ccaacagcct ttgcgatagc tcttaaatct tccaaaagct
      121 ttccgtactc ttctggttta attgactgtt gaccatcaca taaggcatta gcagggtcat
      181 tatgtacttc tattattaat ccatctgcac ctacagctat ggctgccttt gataatggtt
      241 ctaccatcca tgactttcct gctgcatgac ttggatcaac aattactggt aaatgactta
      301 gttttttaat agcaggtatt gcacttaagt ctaaggtatt tcttgtataa gtttcaaagg
      361 ttctaattcc tctttcacaa agaattacat tttcatttcc accagacatt atgtactctg
      421 ctgacattat aagttcctct attgtagctg acaatcctct ctttaaaaga ataggtttat
      481 ttgtctttcc aagctctttt aaaagatcaa agttttgcat atttcttgct ccaacttgaa
      541 taacatcaac atcttcaata aacttttcta tcatactagt tgacattatt tctgtaacta
      601 taggaaggcc tgtttttgcc ttagcctttt ttagaagctc caatccttca agttctaacc
      661 cttggaaact gtatggtgaa gttcttggtt taaatgcacc acctcttaag aaacttgccc
      721 ctaacgcctt aacttctttg gctatttcag ttatttgttc ctcactctct acggaacaag
      781 ggccagctat taatgctatt ttcttatcac caattttatt tcctaagaca tcaattatag
      841 agttctctgg atggaataat ctatttgcct ttttaaaggg ctcttgaaca ggcattattc
      901 tctctacatt tttgtttgct tctactttag caggatctat tttacttgta tctccaacta
      961 aaccaatcac acacatttca cttccagaaa ttctttgagt ctcaactcca agtgattcta
     1021 tcttttttat aaattttaaa atctcctctt cctttgttcc tggttttaat ataactatca
     1081 taattagccc tcctttttta ttttaacctt tttatctaaa atagtctaaa tgtaaaatta
     1141 aactgcttaa attatactat tgcaaaaaaa tagaacccac tttaatgagt tctatcaaat
     1201 aaattctaat acaatattac tataatatta atatctatca cactaattat acaaacccat
     1261 tagttaaaag agaatagttt ttgttgcaca taaaagatcc agagctaaaa tgaaagcccc
     1321 agctagagta aaaactataa aattgtgcta ctaatccctt tactaataaa tttagcataa
     1381 catcctccaa aaacttttta aacagttatc tctaaacttt tttaaattta ttatacactt
     1441 tgtaatttca aagtcaataa aacttaaaac aaaagtagta taagactcta tataaaatct
     1501 tatactactt gcttaaaatt tacttcaatc taattaaaat aattaaaact tattatcttt
     1561 cagttctaac agttattgat tccttagttg aaagttcacc attagaatat ttagcagcaa
     1621 tcttaaagtt ataaatagtg tgtctattta atcccttaaa tgtaaattca gttttatcat
     1681 cacctatttc tttaactttc ttaccatctt tataaagaac atacccttct aatccataag
     1741 ttttttctgg ctcttcccag cttacagtca tatttttctt agtcttatca gtaaccttaa
     1801 agtttcttac tgggtttact atgacttctt cctcaacttc tggatttcca accttaactc
     1861 ttactgtatt tgaagaaatt ttatttccat taacagtaat atttacttgg acatcagtaa
     1921 agccttcaga agctccagtc atcattccat tttcaatagt taaaacttct ggttttgaaa
     1981 ctttatattc aactttagct tcttctaagt taacattttc accattattc attaatgcat
     2041 ttatagcaaa tggaatgctt tgtccaactt ctaagtcatg ttctactgta tttaagttta
     2101 catgagctaa ttcttataat agatactaga taatctacca aaataaaagg ggctgtat
//
LOCUS       DAEVGI010000247         2130 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEVGI010000247 DAEVGI010000000
VERSION     DAEVGI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.249"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(59..739)
                     /locus_tag="K8M77_002489"
     CDS             complement(59..739)
                     /locus_tag="K8M77_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HBI7086923.1"
                     /translation="MVLVLTFILFLMIFLIIVDFFAILFRLTGVPIEKARFQVTSLLT
                     GTGFTTKESEIIVQHPTRRKLAIWLMGFSYASTATIISFAVNIITSSVIDVGSIAVIV
                     LFIIFVLFFHKSSLLEKFENKIEKFIVQSKIWDKINSKHLTLITNTKGFGVYEIYLSN
                     KTTLIGKSIRDSNLKANEIEVLNIDKGDKFVNFPTPDYTFEICDKVTVYGNVKNIKNM
                     FKHINEVK"
     gene            complement(775..962)
                     /locus_tag="K8M77_002490"
                     /pseudo
     CDS             complement(775..962)
                     /locus_tag="K8M77_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471345.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(971..2086)
                     /locus_tag="K8M77_002491"
     CDS             complement(971..2086)
                     /locus_tag="K8M77_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBI7086924.1"
                     /translation="MKLLSLNTEILGFDTSSEFAKELNINGKDLVFTNRRIYEGSFKS
                     LDLKCNYVLKDKYNFNEPNDEIIDEIFKDIKNIDFNRVIAIGGGSILDVGKLLALKDV
                     SSTKKLFKRELPIIRDKELIIVPTTCGTGSEVTNISIVEIKSENTKLGLAVDELYADK
                     GVLIPELCKTMPYKAFVYSSIDALIHSIEGFLSPNSTPYTDLFAFKAIEMILSGYKEI
                     IKKGEEYRNTIMDKFLIASNYAGIAFGNAGVGAVHALSYPLGGKYHVPHGEANYQFLM
                     AVLNFYKKNNGDGKIIELTRLISSIINAKEEDCYLELENLLNNLIRRKNLKEYGMTID
                     EIEKFSHSVMKNQQRLLKNNYVSMEIKDMEEIYKYLY"
BASE COUNT          741 a          356 c          184 g          849 t
ORIGIN      
        1 taataaaact tattttgata cacgcccttt ataaaatatt attttaatat atatattttt
       61 atttaacttc atttatatgc ttaaacatat tctttatatt tttaacatta ccatagactg
      121 ttaccttgtc acaaatttca aaagtataat caggtgttgg aaaattaaca aacttgtccc
      181 ctttatctat atttaaaact tctatttcat ttgctttaag gttgctatct cttatgctct
      241 ttccaataag cgttgtcttg tttgaaagat atatttcata aactccaaat cctttagtgt
      301 ttgttattaa agttaaatgc tttgaattaa tcttgtccca aattttagat tgaacaataa
      361 atttctcaat tttattttca aacttttcta ataaagaact tttatgaaaa aacaaaacaa
      421 atataataaa aagaacaatt acagctatgg aacctacatc tataacacta ctagttatta
      481 tatttactgc aaaagatata atagttgctg tacttgcata actaaatccc atgagccaaa
      541 tagcaagctt tcttctagta ggatgttgaa ctattatttc actttctttt gttgtaaaac
      601 ctgttcctgt caaaagagat gtaacttgaa atctggcttt ttctattggc acccctgtaa
      661 gtctaaataa tatagcaaaa aaatctacta ttattaaaaa tatcattaaa aataaaataa
      721 atgtaagtac taaaaccaca agtacctctc cttcccatta actatttaaa attattatct
      781 tataataata caatatagta atttttcctg ttgttcatca ttcgatttaa tatgttgttc
      841 tttaatatta cttaaatttt acctttcttt taacattcaa ctgctttaaa taaaaaacct
      901 aaaatcaaca aaattacaag caaaagttct ccagcaatta aaatcatcct aaaaattctc
      961 ataatgcctc ctaatataga tatttataaa tttcctccat atcttttatt tccatagata
     1021 cataattatt tttaagtaat ctttgttgat ttttcattac actatgagaa aatttttcaa
     1081 tttcatcaat tgtcattcca tattctttta aattttttct tctaataaga ttatttaata
     1141 agttttctaa ctctaaataa caatcctctt cctttgcatt tataatactg cttattaatc
     1201 ttgttaattc aataattttt ccatctccat tgtttttctt atagaaattt aatactgcca
     1261 ttaaaaattg ataatttgcc tctccatgag gaacatgata ttttcctcct aaaggataag
     1321 ataaagcatg aactgcacca actcctgcat ttccaaaggc tattcctgca taattgcttg
     1381 caattaaaaa tttatccatt atagtatttc tatactcttc accttttttt attatctctt
     1441 tatatccact taaaatcatt tcaattgctt taaaagcaaa taaatctgtg tatggagtag
     1501 aatttggaga taaaaatcct tctatactat gtattaatgc atcaatagaa ctataaacaa
     1561 aagctttata aggcattgtt ttacaaagtt caggaattaa aactccctta tctgcataaa
     1621 gttcatctac tgctaaacct aacttagtat tttcactctt tatttcaaca atagatatgt
     1681 ttgttacctc actaccagta ccacaagttg taggaactat aataagctcc ttatctctta
     1741 ttataggaag ttctctttta aatagtttct ttgtactact tacgtctttt aatgctaaaa
     1801 gtttacctac atctaggatt gatcctccac caatagctat aactctatta aagtctatat
     1861 tttttatatc tttaaaaatc tcatctataa tttcatcatt aggttcattg aaattatatt
     1921 tgtcctttaa gacataatta cattttaaat ctaaagattt aaaacttcct tcatatattc
     1981 ttctatttgt aaaaactagg tcttttccat ttatatttaa ttcttttgca aattcagaag
     2041 aagtatcaaa acctagtatt tccgtattta aacttaaaag tttcatctta atcacctact
     2101 aaatcactaa aatattttaa ctgatcgcac
//
LOCUS       DAEVGI010000248         2081 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEVGI010000248 DAEVGI010000000
VERSION     DAEVGI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.250"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            417..1637
                     /locus_tag="K8M77_002492"
     CDS             417..1637
                     /locus_tag="K8M77_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBI7086925.1"
                     /translation="MNKEIKLRNDIYSVGVMDNRPVPFHRLILEKGTTYNSYLLKTKK
                     PTVIDTVDLIYTKDFFNNLKEIIDLKDISYIVINHTEPDHSGALGGLARQATNAKIVC
                     SEKAVYHLKELYKLHDRDFIVVNDGDTLDIGGKTLLFKMTPYLHTEETMITYDVEDKI
                     LFPCDIFSTHLTAKEHFSSKSNEDIIEDFLTYYKLIMEPHRKYIRPLIDILKDLDIEV
                     IAPSHGYILDENIQSYIDIYDKYSKETKKGKKATIVYTTMRNTTKKIAEKFKSELENE
                     DIEVYIFNADKSTKEDILASIKESDSILFGTSTKYGDLIGSIEDILKELPKLDLSNKL
                     LGAFGSFGWSGEPIEIIQDYLNSTNGNVLNTSSIIKSTGMIDVHFPVRIRFSLNEESE
                     KEVVRAADYISSII"
     gene            1664..1825
                     /locus_tag="K8M77_002493"
     CDS             1664..1825
                     /locus_tag="K8M77_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HBI7086926.1"
                     /translation="MKKFICDVCGYIYDPTVGDPDNGVEPGTEFKDIPDDWVCPLCGV
                     DKSQFSETE"
BASE COUNT          814 a          240 c          297 g          730 t
ORIGIN      
        1 tatatcataa tataaatata aattacaata tatttaaaac atagaaattt aagaacataa
       61 tatctcttca cctgatttcc atttagctag ataaacttaa tctccctgtt ctgcaacaga
      121 ctgaggtact tgagcctttc ttaataattc ttcagaattt cctcaaattc cgttatcatg
      181 gattttgtat gcttttatct cttcttatgg tgtaattaag aaagagactc ttaaaactaa
      241 tccttattct ataaatttct tgtaattaat aagtattatt ttaaaaaaag taaataattt
      301 ttagaaaatt tttatattat ttttatcatg tgtgtgcaat cacattcaga tattttatta
      361 tgttttaaac taaggttgta gattttcaca aaagaaactt aataaggagg gtacttatga
      421 ataaagaaat taaactaaga aatgatattt actcagtagg agtaatggac aatagacctg
      481 ttccatttca tagattaata ttagaaaaag gaactacata caatagttat ttattaaaaa
      541 ctaaaaagcc aacagttatt gatactgtag atttaatata tacaaaagat ttttttaata
      601 atttaaaaga gattattgat ttaaaagata taagttacat agttattaat cataccgaac
      661 cagatcattc aggtgcatta ggtggactag ctagacaagc tactaatgct aaaatagttt
      721 gcagtgaaaa agccgtatat catctaaaag aattatataa attacatgat agagatttta
      781 tagtagtaaa tgatggggat acccttgata taggaggaaa aacattatta tttaaaatga
      841 ctccttatct tcacactgag gaaacaatga ttacatatga tgtagaagat aaaatattat
      901 ttccttgtga tatttttagt actcatctta ctgccaagga acatttctca agtaaatcaa
      961 atgaagatat tatagaggac tttttaactt actataaact tataatggaa ccacatagaa
     1021 aatacattag gccactgatt gatatattaa aagatttaga tatagaagtt attgctccat
     1081 cacatggtta tatactagat gaaaatattc aatcatatat tgatatatat gataaatata
     1141 gtaaagaaac taaaaaaggg aaaaaagcta ctatagttta cactacaatg agaaatacaa
     1201 caaaaaaaat agctgaaaaa ttcaaatctg aattagaaaa tgaggatatt gaagtttata
     1261 tatttaatgc agataaaagt actaaagaag atatattagc ttcaataaaa gaatcagatt
     1321 caatactatt tggtacttca actaaatatg gagatttaat aggttctatt gaagatatat
     1381 taaaagaatt acctaaatta gatttaagca ataaactttt aggtgctttt ggttcatttg
     1441 gatggagtgg tgaacctata gaaataattc aggattactt aaactctaca aatggtaatg
     1501 ttttaaatac ttcatcaata ataaaatcta ctggtatgat tgatgttcat ttcccagtaa
     1561 gaattagatt ctcactaaat gaagaatcag aaaaagaagt tgtaagagct gctgattata
     1621 tcagttcaat aatataatag ttaaatttta ggaggaatta attatgaaaa aatttatatg
     1681 tgatgtttgt ggatacattt atgatccaac tgtaggagat cctgataatg gtgttgaacc
     1741 tggaactgaa tttaaagata tacctgatga ttgggtttgc cctctttgtg gagtagataa
     1801 atctcaattc tctgaaactg aataatatta tattagtaaa agagggtgta atcaaaatga
     1861 ttaatttaat cattttgatt acgtcttctt ttttgactgt tgaataaaaa tcctcactaa
     1921 atttaataaa aaaggtcata acaaatagaa gaatagtcac attcttctaa attctggtgt
     1981 ttatctggtt ttaagctata ttttttaaat gcagtaattg cccgttacgc ttttcaagcg
     2041 ttttacgatg taatttattt atgttatatc caaaagcaat t
//
LOCUS       DAEVGI010000249         2033 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEVGI010000249 DAEVGI010000000
VERSION     DAEVGI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.251"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..587
                     /locus_tag="K8M77_002494"
     CDS             <1..587
                     /locus_tag="K8M77_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086927.1"
                     /translation="VSIVHLKNSTYLQNINNSILENKSNSKILEDNIKEYKIPIFLSQ
                     LSIIDIFKGNIQFPEHITHINSIKNTLKLNSSSLILDASSRKSGFLYLDGHLEIDLTY
                     SIPENNKIPLISKINNYIIRIPFNTSIHVNFVYPLMGNNIYFNKKNISLKETKFTTDI
                     KYENSKLLKNCIKLHKTSKLSIISNYFINIYDFL"
     gene            692..1429
                     /locus_tag="K8M77_002495"
     CDS             692..1429
                     /locus_tag="K8M77_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086928.1"
                     /translation="MSSCCESKFPVCADDIDVSVDNFDTTLGNNPSTVGNAKALVRVA
                     AVNINHPLTDSITIPEGFYSIKGISRRLVLTQCYIVPISANRVTSGQLFVEGYILKNV
                     QYATPIDNENSSEPCEDCVVMRNDYKDLTAKINFNFSVPITLTNANIVNPTNEVERAI
                     LKDCMQPCDNGTIGESDCERLFSQEVVLQEPFSCELDRYTINEAVISKTNCSLTNENL
                     FDTIIEKLNINLVISIFQLRPVTVTIP"
     gene            complement(1499..2011)
                     /locus_tag="K8M77_002496"
     CDS             complement(1499..2011)
                     /locus_tag="K8M77_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI7086929.1"
                     /translation="MEFRQAKISDLDQIVEIIELSKKYLKETKVDQWQNGYPAKEDLR
                     RDIESGNSYVLTNKDEIVATTLISLEGESTYNSIFNGEWITNEEYIVMHRVAVHEKYK
                     GKGIFKELIKEAESLALNKGIFSIKIDTHRDNISMQKALLKSSFEKCGIIYLDDGSER
                     IAFEKVIKFF"
BASE COUNT          779 a          293 c          206 g          755 t
ORIGIN      
        1 atgtatcaat tgttcatcta aaaaattcta cttatctgca aaatataaat aatagtattt
       61 tagaaaataa atcaaattca aaaatattag aagataatat taaagaatat aaaataccta
      121 ttttcttaag ccaattgtct ataattgata tatttaaggg aaatatacaa tttcctgaac
      181 atattaccca tataaattca ataaaaaaca ctctaaaatt aaattcaagt tctttaattt
      241 tagatgcatc tagtagaaaa tctggattct tatatttaga tggtcattta gaaatagatt
      301 taacatattc aataccagaa aacaataaaa ttcctcttat atctaaaata aataactata
      361 taattagaat tccttttaat acttctattc atgttaactt tgtttatccc ctaatgggca
      421 ataatatata ttttaataaa aaaaatatat cattaaaaga aactaaattt actaccgata
      481 taaaatatga aaattcaaag cttcttaaaa attgtataaa actacacaaa acatccaaat
      541 tatccataat atctaattat tttattaata tatatgattt tttgtaaaaa ttttaaagtt
      601 taaattacaa aaatagaacc ttttaaataa atatagaata atatatagta taaaatcatt
      661 aattaaaatt tatattatga ggtgaagtta aatgagttca tgttgcgaat caaaatttcc
      721 tgtttgtgct gatgatatag atgtaagtgt tgataatttt gatacaacat taggtaacaa
      781 tccttcaact gtcggaaatg ctaaagctct agttagagtt gctgctgtaa atataaatca
      841 tcctttgaca gattcaataa caattcctga gggattttat agtataaaag gtatatctag
      901 aagattagtt ttaactcaat gttacatcgt tccaatttca gcaaatagag taacaagcgg
      961 ccaacttttt gttgaaggat atattctaaa aaacgtacag tatgctacac caattgataa
     1021 tgaaaattct tctgagccat gtgaagattg tgttgtaatg agaaatgact ataaagattt
     1081 aacagcaaaa attaacttta atttttcagt tccaataact ttaactaatg ctaatatagt
     1141 taatccaaca aatgaagttg aaagggcaat attaaaagac tgtatgcaac cttgcgataa
     1201 cggtactata ggtgaatctg attgcgaaag attattttca caagaagttg tactacaaga
     1261 accattttcc tgtgaattag atagatatac aattaatgaa gctgttataa gtaaaactaa
     1321 ttgctcactt actaatgaaa atctatttga tacaataata gaaaagttaa atataaattt
     1381 agtaatttct atatttcaac ttagaccagt cacagtaaca attccttaat actattttat
     1441 tattaattca acacaaaata ctggaaaatt ataaatttcc agtatttttt tatctatttt
     1501 aaaaaaattt aattactttt tcaaaggcta ttctttcact tccatcatct aaatatatta
     1561 ttccacattt ttcaaaacta ctttttaata atgctttctg catagaaata ttatctctat
     1621 gagtatctat ttttatgcta aaaatccctt tatttaaagc caaactctct gcctctttta
     1681 ttaattcttt gaaaattcct tttcccttgt atttttcatg aacagcaacc ctatgcataa
     1741 caatatactc ttcattggta atccattccc cattaaatat tgaattatag gtactttcac
     1801 cttctaaaga tattaaagtt gtagcaacaa tttcatcttt attagttaat acataactat
     1861 ttccactttc tatatctctt cttaaatcct cctttgctgg atagccattt tgccattgat
     1921 caaccttagt ttctttaaga tattttttag ataattctat tatttccaca atctgatcta
     1981 aatcagaaat ctttgcttgt ctaaattcca aatttactcc cccctaattt cta
//
LOCUS       DAEVGI010000250         2015 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEVGI010000250 DAEVGI010000000
VERSION     DAEVGI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.252"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(52..747)
                     /locus_tag="K8M77_002497"
     CDS             complement(52..747)
                     /locus_tag="K8M77_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI7086930.1"
                     /translation="MNNFTYKEVYIEDGKRVLEINVLPEKYCNFDCIFCPIGRSKNKI
                     DTQKSFDNVDESLIELENMINDTKPDLIFINSKGEALINDKIENIIDFIKSKGVPVRL
                     LSNGYILGKDEYIKVANKCDEVIGEIKVITEEDFKKVQRPIEGYTLHEYISNMASFNK
                     QYNGKFIFEITIVKGYNDDPESVNKLKEVIKTICPNEVIVARIDDDIFKKKLGISDER
                     FEEISREILDVNC"
     gene            complement(769..999)
                     /locus_tag="K8M77_002498"
                     /pseudo
     CDS             complement(769..999)
                     /locus_tag="K8M77_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1378..1935
                     /locus_tag="K8M77_002499"
     CDS             1378..1935
                     /locus_tag="K8M77_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086931.1"
                     /translation="MKKRIERLIIFCMLITITIPNIVYAKTNMRYEKEKTNIVEPYGP
                     KIEDLKSKDVIINNLQEIKRIRRNLTAVNISESSTPNELKDIYNRLDFYIQEFIEIKK
                     NLDNNIKTYTNSFSDKFFSEQVLFIAESYIVSLRQQQNLIIALQEEKVEAKKLVYSSY
                     LIPIYHYITLGDQMTAYVDTYFVVI"
BASE COUNT          759 a          290 c          209 g          757 t
ORIGIN      
        1 attgatatat acttattaaa tttagaactt tgaatctaat aatctatata tttagcagtt
       61 tacatctaat atctcccttg aaatttcctc gaatctttca tcagatatac caagtttttt
      121 cttaaatata tcatcatcaa ttctagcaac aataacctca ttagggcaga ttgtttttat
      181 aacttccttc agtttattaa ctgattcagg atcatcatta tatcccttaa ctatggttat
      241 ttcaaatatg aattttccat tatattgttt attaaaggaa gccatatttg atatgtattc
      301 atgaagggta tatccttcaa ttggtctttg aaccttctta aaatcttcct ctgttattac
      361 ctttatttct ccaataactt catcacattt gttagcaacc tttatatatt catcctttcc
      421 taggatatac ccattagaaa gcagtcttac aggaactccc tttgatttta taaaatctat
      481 aatattctca attttatcat ttattaaggc ttctcccttt gaattaataa agattagatc
      541 aggttttgta tcattaatca tattttctag ttcaattaat gactcatcca cattatcaaa
      601 ggatttttga gtatctattt tattctttga tcttcctata ggacaaaata tacaatcaaa
      661 attacagtac ttttctggaa gcacattaat ctctaaaact ctctttccat cctcaatata
      721 aacctcttta taggtaaaat tattcataaa cttatcctcc gtaaataatt aaattatagc
      781 ttccttatta ttaatatgaa gtattgaaac ctcagatttt ccacctaagt ttttctctac
      841 taattgagat aactctttta aggcattttc aaattctttt tctctatttt catcaactaa
      901 ctcaatgcat aagaaagcat tttttgtgtc ttctgttaac atagttctta ctgactcacg
      961 acaattccag catctgcaaa tagctccttc atcatctttt tcacaacaat atacatcctt
     1021 taaaataaaa aagccaaaga aatattataa aaatatctcc caggctttta tccttccgtg
     1081 ttcacagtct ttctgtgcgt tttctcttgg accagactaa ctaaaaagct acggaaccct
     1141 agaaaacttt atattattaa gttttaatta acaaattcaa aattataagt taattttagc
     1201 atattataat tgctaaatca aattaaatga attattatac atatttcaat tacaattaaa
     1261 aaacctatat gaaaaatttc aaatataaaa ggttatatta atttttacaa gaataaaata
     1321 ttaagaatta ctaaaaatag atatagtgtt aaaagttatt atagaggagt tggaataatg
     1381 aaaaagagaa tagaacgttt aataattttt tgtatgctta taactataac tattcccaat
     1441 atagtatatg caaaaactaa tatgagatat gaaaaagaaa aaacaaatat tgtagaacct
     1501 tatggcccta aaatagaaga tttaaaaagt aaggatgtta ttataaataa cttacaggaa
     1561 ataaagagaa taagaagaaa tttaactgct gttaatataa gtgaaagttc tacacctaat
     1621 gagttaaaag atatatataa tcgtttagac ttttatattc aagagttcat tgagattaaa
     1681 aaaaatttag ataataatat aaagacatat acaaattcat tttcagataa atttttttct
     1741 gagcaagttt tatttatagc tgaaagttat attgttagtt taagacaaca acaaaactta
     1801 ataattgcat tacaagaaga aaaagtagag gcaaaaaaac tggtttattc aagttattta
     1861 atacctatat atcattatat tactttagga gaccaaatga cggcatatgt tgatacttat
     1921 tttgtagtaa tataagtgca ttaaagaatt tatttatagc taataataaa tatttaaaac
     1981 tagataacat ttaacataaa cttaatttta ttatt
//
LOCUS       DAEVGI010000251         2010 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEVGI010000251 DAEVGI010000000
VERSION     DAEVGI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.253"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(246..1409)
                     /locus_tag="K8M77_002500"
     CDS             complement(246..1409)
                     /locus_tag="K8M77_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBI7086932.1"
                     /translation="MKKLFNNLIFKLILGVILGIIIGTYSSEGLMSTIVTIKYVLGQI
                     IFFSVPLIILGFIAPSIAKLKDNASKLLGYAVLIAYLSSVFAAILSMIAGYALIPKLS
                     IVSNIASLKELPELIFKLDIPPVMSVMSALALALLLGLAVGWTKADLVEKLLDQFQAI
                     VLSIVNKIIIPILPFFIATNFAALAYEGGLSNQLPVFFKVILIVLFGHFIWLTILYLI
                     GGAISKENPWEVVKYYGPAYLTAVGTMSSAATLPVALESAKKSKALREDIVDFAIPLC
                     SNIHLCGSVLTEVFFVMTVSQILYGKIPSLPTMILFIVLLGVFAIGAPGVPGGTVMAS
                     LGLIISVLAFDEAGTALMLTIFALQDSFGTACNVTGDGAIALMLTGIAKKKNL"
BASE COUNT          741 a          334 c          207 g          728 t
ORIGIN      
        1 tttaaagtta aattctataa ataattataa aaatatacac ttatttataa attctcttac
       61 tatttatttt agtcaaatct tgtatttttg tgaatttaaa cacattattt attagttttt
      121 aaaattacgc aaattatata tctattgatt taaagtgatt atttttaccc acttaatttc
      181 atttatactc cctattattt aattgaaaaa taaaaaggca tactataaaa gtacaccttt
      241 ttgtcttata aattcttttt ctttgctata cctgtcagca taagagctat tgctccatca
      301 ccagttacat tacatgctgt tccaaaacta tcttgaagag caaatattgt taacataaga
      361 gctgtaccag cctcatcaaa ggctaataca ctaattatta aacctaatga tgccattaca
      421 gttccccctg ggactcctgg tgcccctatt gcaaacactc ctaataatac tataaacaat
      481 atcatagttg gtaaactagg aatcttacca tataaaattt gagatactgt cattacaaaa
      541 aatacctctg taagaactga accacataaa tgtatatttg aacataatgg tattgcaaaa
      601 tcaactatat cttctcttaa agcctttgat ttctttgcag actctaaagc tactggtaat
      661 gttgctgcac ttgacattgt tcctacagca gtaagatatg ctggtccata gtattttaca
      721 acttcccatg gattttcttt tgatattgct ccacctatta aatataaaat tgttaaccat
      781 ataaaatgac caaataatac aattaatata actttaaaga atacaggaag ttgattactt
      841 aatcctcctt catatgctaa agctgcaaag ttagttgcta tgaaaaatgg taatattggt
      901 attattattt tatttacaat actaagtact atagcttgaa attgatctaa aagcttttct
      961 actaaatcag cctttgtcca tccaacagct aatcctaaaa gtaatgctaa agctaatgca
     1021 ctcattacac tcataactgg tggtatatct aatttaaata taagttctgg taattccttt
     1081 aatgatgcta tattagatac tatagataat ttaggtatta atgcatatcc tgcaatcatt
     1141 gaaagaatag cagcaaaaac tgaagataaa taagctatta aaacagcata tcctaataat
     1201 ttgcttgcat tatcttttaa cttagcaata gatggcgcta taaaccctaa aataataagt
     1261 ggaacagaga aaaatataat ttgtcccaat acatacttaa ttgtcacaat tgttgacata
     1321 agcccctctg aagagtatgt gcctattatt attcctaata taacacctaa aattaatttg
     1381 aaaataagat tattaaataa tttcttcaca atttaccccc ctttacttta tattttctat
     1441 gtaaagaatt atttataaac ataataaaaa aaaacttcta ccccctatac agaggcagaa
     1501 gtaaaattcc acggttccac tctgatttta agtagtaagc taagttaaac tacaaaatct
     1561 ctttaggtac aaatatacca ctctgctata acgggcatac ccgcattccc ctactataat
     1621 ctaaaattat ttctggaaac aagctcatag atgcactcat gaaaaaattc tttaaagtct
     1681 tttacagcct agaagactct ctctgaataa agaaattaat tctacttttc taatcacagc
     1741 ttttcatatt ataaaagtta attcttagta ttcttatacg aattacctat attatatttt
     1801 aaactctgca cttagtcaac ataaatttta aaaaatgtaa acaaaaaaag taatatatta
     1861 ataaatatta cctttttaaa ttctaaatat tttgttgtat tttaaatata atctattagt
     1921 aatactactt taaccttcta ctatttttca ttattctaaa taatgctaaa taaagtttta
     1981 tatttaatca tttgttcatt ttatatacat
//
LOCUS       DAEVGI010000252         1998 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEVGI010000252 DAEVGI010000000
VERSION     DAEVGI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.254"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(378..1325)
                     /locus_tag="K8M77_002501"
     CDS             complement(378..1325)
                     /locus_tag="K8M77_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBI7086933.1"
                     /translation="MKNYVVGIDLGGTKISCALADLEGNVKAQHTTPTNAHEGEQAVL
                     DRIIGCVETVICEGKVTIDEVEAIGIGSPGPLDARTGIIITTPNLPFKNFNLVSPLKA
                     KFGIPVYLDNDANVAAIGEFMLGAGKGTENMIYITVSTGVGGGAILNGKIYRGSTSNA
                     LEIGHSTVAPGTVRCNCGNMGCLEAVSSGTAIGKRGREAVATNVETSLKDYDNVTSYE
                     VFVEAAKGDRVAKSIIDEALNYLGIGVANAIATFDPDMVVIGGGVSKAGEVVFETVQE
                     VVNERCFKAMAEHCKIVPAGLGTDAGVIGAVALALLECK"
     gene            complement(1577..1813)
                     /locus_tag="K8M77_002502"
     CDS             complement(1577..1813)
                     /locus_tag="K8M77_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086934.1"
                     /translation="MCNYRMDIKGKISLSDYSNIFDYINIVDVCDRFMIYMESLDDDN
                     IGIICSMLKDNNFNIKNQGYDNQGIYYINAKKIG"
BASE COUNT          671 a          360 c          190 g          777 t
ORIGIN      
        1 taaaattttt ttattttcta caaatgaaac cttaatactt tttaaaatct tacttttacc
       61 tatatagtca taattatcta aaacattgtg ttttatagta tagaagttat ttatagtata
      121 aaagacatta tataatgaac ttatataatg tctttataat aatcacaata tgtggttatg
      181 acctaacttc atacttaatt taccattttc aatatagttt tatcacaaaa taaccttttt
      241 ttctagtatt taattattta aatttgcgag tttataaatt tataacattt tactatgtaa
      301 acattatatt tgtctagcaa aatatataaa aaaataaagt tagaagtaaa aacttctaac
      361 ttatttatat aaatttatta tttgcactct aataatgcta aagctactgc tccaataact
      421 cctgcatcag ttcctaatcc agcaggaact attttacaat gctcagccat agctttaaaa
      481 catctttcat taacaacttc ttgaactgtt tcaaaaacaa cttctccagc ttttgaaact
      541 cctccaccta taacaaccat gtctgggtca aaagttgcta ttgcatttgc aacaccaatt
      601 cctaagtagt ttaaagcttc atctattatt gattttgcaa ctctatcacc tttagctgct
      661 tcaacaaata cttcatatga agttacattg tcgtaatctt ttaagcttgt ttctacattt
      721 gtagcaactg cttctcttcc tcttttacca atagctgttc ctgatgatac agcttctaga
      781 catcccatgt taccacaatt acatcttaca gttccaggtg caacagttga atgtccaatt
      841 tctaatgcgt ttgaagtact tcctctgtaa attttaccgt taaggattgc tcctccacct
      901 acaccagtac ttacagttat ataaatcata ttttcagtac cttttccagc acctaacata
      961 aattcaccta tagcagctac gttagcatca ttatctaagt aaacaggaat accaaactta
     1021 gcttttaatg gtgaaactaa gttgaagttt ttgaaaggta aatttggagt tgttattatt
     1081 ataccagttc tagcatctag tggtcctggt gatccaatac ctattgcttc tacttcatca
     1141 atagttactt ttccttcaca tattacagtt tcaacacagc ctataattct atctaaaact
     1201 gcttgctctc cttcatgagc attagttgga gttgtatgtt gagctttaac atttccttct
     1261 agatcagcaa gagcacagct aatttttgtt ccccctagat ctattccaac aacgtaattc
     1321 ttcatttaaa aaatcctccc ccatgttcct tatggattaa ttcaactata agtgcttctt
     1381 acaagttgcc ctttagaatt cacttcgtta aattattata tgacaactta aattatagct
     1441 tattaataat tttatttaac acaaagtaca ttataacata aatgtacgta cctttacaca
     1501 tttaaagtta taccattttt aaattatata cttttacaat tatttatata aaaaatacta
     1561 ttatggcttg ctttcactaa cctatttttt tagcatttat atagtaaatc ccctgattgt
     1621 cgtatccttg attttttata ttaaaattat tgtccttaag catagaacaa ataataccta
     1681 tattatcatc atctaaagac tccatataaa tcataaatct atcacataca tcaacaatat
     1741 ttatataatc aaagatgttg ctataatcac ttaaacttat tttacctttt atatccattc
     1801 tataattgca cataaattac acctcattta agatagtatc tctaaatata taattagata
     1861 tataaatata agatccctct catatttcaa aagatttaat tatttttaat ctttgaatta
     1921 tttcacttta atttaagata tttttcagtt actaataatt ttaattaaat taaatccttg
     1981 agcttttaga aagtacct
//
LOCUS       DAEVGI010000253         1989 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEVGI010000253 DAEVGI010000000
VERSION     DAEVGI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.255"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            437..1906
                     /locus_tag="K8M77_002503"
     CDS             437..1906
                     /locus_tag="K8M77_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI7086935.1"
                     /translation="MGKDKKIKLWALVMLIFVPTFGFGNIASNAVYLGPAAIPSWVIV
                     SILYFLPLCGIIAEMASANRDKEGGIYSWINKALGEKWAFVGTWTYFIGILFYLQMVF
                     SRIPVAASWAILGRNIFTDSNAYLLPILSIVICIAMTYVATIGVSKFSKLADFGGQFT
                     LAATVIFILMAIAGYFIGKPSATEFTVQNIIPDFSVSYFSTFSWLLFAVSGSEVAGTY
                     IMQTENPKKTFPKAMIIATILIALSYILGSVAIQFIASPEVLQKAGIQDAGYVVYYIL
                     ANNFGINGKVIVQVYAAINLITSIAAYIIWMESPIRAMFGEVPKGTFPSFLTKKRKDG
                     TLVNALWTQCAILVVLISIPLLGIGSINDFFKLLTDLSSLAVVVPYVVLICAFISFRK
                     HNKDLDFKFFKSDALAYTVAGIALVLSCAGFFGAGLQDIVGSSGKDATILIIKTYGGP
                     VILMALGLVLRALSEKSYKNKSLGNENIEVEIVEESICE"
BASE COUNT          745 a          209 c          332 g          703 t
ORIGIN      
        1 tttaaatata ttatgaaaga atagcaggtt ataaatattt ttttagaaag tttaaatata
       61 taaataagtt ggaatatata tgaacctata gaataaatga gatgtttaat taagaaataa
      121 agaaaactaa taaaaaggtt aaaaatgatt atagatatat ttaattagtc tttaattata
      181 tctacagtca ttttttattt taaaattata caaaaaatac ataaaaataa gcgtatttta
      241 ggtaaaatac ttaataaaat aagaaattag gtttaaaata acggtttcat aaataaatat
      301 acaaaaaata tgcgatttta ttcaaaataa gtattgtata tttattatag gcgtagtata
      361 attttaacat aaatatgaac taaaaattaa taaatattga ataaagttta aaaatataag
      421 ctaaggagag tttaaaatgg gaaaggataa aaaaataaag ttatgggcat tggtaatgct
      481 tattttcgta ccaacatttg gttttggtaa tatagcaagt aatgctgtgt atttagggcc
      541 agcagctatt ccatcgtggg taatagtatc tattctttat ttcttacctt tatgtggaat
      601 aatagcagaa atggcttcag caaatagaga taaagaaggt gggatatatt catggataaa
      661 taaagcttta ggtgaaaaat gggcttttgt gggaacttgg acttatttca tagggatatt
      721 attttattta caaatggttt tttcaagaat accagtagct gcatcatggg caatacttgg
      781 aagaaatata tttactgatt ctaatgcata tttattacct attttatcaa ttgtaatatg
      841 tatagcaatg acatatgtag ccactatagg agtaagtaag ttttcaaaac ttgctgactt
      901 tggaggacaa tttactttag cagcaactgt tatatttata ttaatggcaa tagcaggata
      961 tttcattggc aaaccttctg caacagaatt tacagttcaa aatataattc cagattttag
     1021 tgtgagttat ttttctacat tctcatggct actatttgca gtgtcaggat cagaagttgc
     1081 aggtacttat ataatgcaaa ctgaaaatcc taaaaagaca tttccaaagg ctatgataat
     1141 tgcaacaatt ttaatagcac tctcatacat tttaggttct gtagctatac agtttatagc
     1201 atcaccagaa gttttgcaaa aggcaggaat tcaagatgcc ggttacgttg tatattatat
     1261 attagctaat aatttcggaa taaatggtaa ggttatagta caagtatatg cagctattaa
     1321 tttaataaca tcaatagcag catatataat ttggatggaa tcaccaataa gagccatgtt
     1381 tggagaagtt ccaaagggaa catttccaag tttcttaact aagaagagaa aagatggaac
     1441 tttagtaaat gctttatgga cacaatgtgc aattttagta gtattaatat caataccttt
     1501 attaggaata ggatcaatta atgatttctt taaattatta acagatttat catctctagc
     1561 agttgttgtt ccatatgtag tcttaatatg tgcatttata tcttttagaa aacacaataa
     1621 agatttagac tttaaatttt ttaaaagtga tgctttagca tacacagttg caggaatagc
     1681 tttagtactt tcttgtgctg gattctttgg tgctggcctt caagacattg ttggaagttc
     1741 agggaaagac gcaacaattt taataataaa aacttatggg ggacctgtta ttcttatggc
     1801 tttaggtctt gttttaagag ctttatctga aaagtcatat aaaaataaga gtttaggcaa
     1861 tgagaatatt gaagttgaaa ttgttgaaga gagtatatgt gaatagatta agaaatagtt
     1921 ttttaaaatt aaatatggta attttattta agtagaaaag atggattaaa gaacttatcc
     1981 atctttttt
//
LOCUS       DAEVGI010000254         1987 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEVGI010000254 DAEVGI010000000
VERSION     DAEVGI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.256"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            173..1705
                     /locus_tag="K8M77_002504"
     CDS             173..1705
                     /locus_tag="K8M77_002504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086936.1"
                     /translation="MADFAVQMYIGEHKRDASSKVRGFIYQDLLAIEYLIESNDNSSL
                     ELYSEWAEDIYVETNESVSIIQVKYYTSSNINFKEIYNELFYQYVRLKLLDCNKNIKC
                     KLSYYSSKLKKGVGTSEHIKNNINVVEDVEWENIDKVKILEQIYKDEKGNERTKENRE
                     KKLFEVASNEALLNEFINNIYCEDLRKENLEELRNNLKEKIYFLIKSDERAIKINEWE
                     EEVLSEVGLAVSVVYLQESYDEARKNSRLRKRTLNELTDKLIDITNNIDSPSNITNIL
                     KYYVDECLIEYILEDDSLENIEYEEINDIRKFYENLAASTKYFFDKYLLTDLGQYALL
                     NTISKSTSNRLNIESYMKKSYVEKNNIFLEHKVEFMSFLKNVWKVLYSLECKDFSEYI
                     EKAVTDHIQFKFPKQDKIIIMSAIGIDNPVKDIKNIFNRYRLLDKKPKKWLVRNNKIR
                     GIKNYSLDVKSISKELENNTAYISDEVFEIECMECIKLEGYIKDKDNCDECIFSIKCP
                     KR"
     gene            1722..>1987
                     /locus_tag="K8M77_002505"
     CDS             1722..>1987
                     /locus_tag="K8M77_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086937.1"
                     /translation="MEWIKIFKNYTDIKEKVLIYSFCDKEGKWFPELEHIFLHNTKSE
                     MFIVIDLTKKISEISNFYNERYGQVCNKYEKLIEIIIKNWEKSI"
BASE COUNT          896 a          133 c          318 g          640 t
ORIGIN      
        1 attttagata taaatataat gcaatctttg aaaaaataaa tgaaggatat tattgaacta
       61 tatataaaat atggtaaaat atatgggtta ttatatataa atatacaaat tttagtggat
      121 catatccatg tgtataatat agtggaaaaa tatattaaaa aaggggaaaa atatggctga
      181 ttttgcagta caaatgtata taggagaaca taaaagagat gcatcatcaa aggttagagg
      241 ttttatatat caagatttgc tagcgataga atatctcata gagtcaaatg acaattctag
      301 tttagagtta tattcggaat gggcagagga tatatatgta gaaactaatg aatccgtttc
      361 tataatacaa gtaaaatatt atacaagctc taatataaat tttaaagaaa tttataatga
      421 gctgttttat caatatgtaa gattaaagtt gcttgactgt aataaaaaca ttaaatgtaa
      481 attgtcatat tattcaagta aactaaaaaa aggagtagga acttcagaac atataaagaa
      541 caatataaat gtcgttgaag atgttgagtg ggaaaatatt gataaagtca aaatattaga
      601 gcaaatttat aaggatgaaa aaggaaatga aagaacaaaa gaaaatagag aaaaaaaact
      661 ttttgaagta gcttcaaatg aagcattatt aaatgaattt ataaataata tttattgtga
      721 agatttaaga aaagaaaatc tagaggaact tcgtaataat ttaaaagaaa aaatatattt
      781 tttaataaaa agtgatgaac gagctattaa aataaatgag tgggaagaag aggtgctatc
      841 agaggtaggt ctagcagtat cagtggttta tttacaggaa tcttatgatg aagcaagaaa
      901 gaattctaga ttaaggaaaa gaacattaaa tgaattaact gataaattaa tagatataac
      961 aaataatatt gattctccta gtaatattac caatatatta aaatattatg tagatgaatg
     1021 tttaattgaa tatatattag aagatgattc attagaaaat atagaatatg aagaaataaa
     1081 tgatataaga aagttttatg aaaatttagc agcttcaaca aagtattttt ttgataaata
     1141 tttacttact gatttgggac aatatgcgtt acttaatacc attagtaaaa gtacatcaaa
     1201 tagattaaac atagaatcat atatgaaaaa atcatatgta gaaaaaaata atattttttt
     1261 agaacataag gttgagttta tgtcattttt aaaaaatgta tggaaagtac tatatagttt
     1321 ggaatgtaaa gatttttctg aatatataga aaaagcagtc actgatcata tacagttcaa
     1381 atttcctaaa caagataaaa ttataattat gtctgcaata ggaatagata atcctgtaaa
     1441 agatattaaa aatattttta atagatatag attgttagat aaaaagccca aaaagtggtt
     1501 ggttagaaat aataaaatac gtggaattaa aaattatagt ttagatgtaa aaagtatatc
     1561 aaaggaatta gaaaataata ctgcatatat ttcggatgaa gtatttgaaa ttgagtgtat
     1621 ggaatgtata aagttagaag gttatataaa ggataaagat aattgtgatg aatgtatttt
     1681 ttcaataaaa tgtcctaaaa gatgaaagga attaaataat tatggaatgg attaaaatat
     1741 ttaaaaatta cactgatata aaagaaaaag tactaatata ttcattttgt gataaagagg
     1801 gtaaatggtt tccagaatta gaacatatat ttttacataa tacaaagagt gaaatgttta
     1861 tagttattga tttaactaaa aaaatatctg aaataagtaa tttttacaat gagagatatg
     1921 gacaagtttg taataagtat gaaaagttaa tagaaattat aataaaaaat tgggaaaaat
     1981 cgatatt
//
LOCUS       DAEVGI010000255         1960 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.257, whole genome shotgun sequence.
ACCESSION   DAEVGI010000255 DAEVGI010000000
VERSION     DAEVGI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.257"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            77..217
                     /locus_tag="K8M77_002506"
     CDS             77..217
                     /locus_tag="K8M77_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086938.1"
                     /translation="MSKKKTLNGIQQSMKQHNAESSNSKTGFNERSNTAKEPGIANTK
                     GL"
     gene            582..1877
                     /gene="eno"
                     /locus_tag="K8M77_002507"
     CDS             582..1877
                     /gene="eno"
                     /locus_tag="K8M77_002507"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBI7086939.1"
                     /translation="MKQYIEIIDVVARQILDSRCFPTVEVEVYLEDGTVGRAAVPSGA
                     STGIYEAVELRDGDKDKYLGKGVEKAVANVNDTIAEEIIGLNVLDQAYIDKTLIELDG
                     TKNKGKLGANAILGVSLAVAQAAANYLGMPLYQYIGGVNAKVLPVPMMNIINGGSHAD
                     NSVDIQEFMIMPVGFDCFERAVRACAEVYHALKKTLNSKGYSTGVGDEGGFAPNLKSN
                     AEAIEVILEAIEKAGYEPGKEFFIAIDAASSEYYKDGKYVLEHEGKTLTAAEMVDFFE
                     DWVNKYPIISIEDGMAEEDWEGWKLMTERLGKKVQLVGDDLFVTNTERLKTGIEKGIA
                     NSILIKLNQIGTLTETLNAIEMANRAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKT
                     GAPARSERVAKYNQLIRINEELGEVAEYRGRNAFFNLSK"
BASE COUNT          806 a          213 c          372 g          569 t
ORIGIN      
        1 caaggcgagg ttctatgaag aaggaacaat ctccatatgg atatatttga ccatattttg
       61 agtagcagaa tagaaaatga gcaagaaaaa aactttaaat ggaatacaac aaagtatgaa
      121 acagcacaat gctgaatctt caaattcaaa aactggattt aatgagagaa gtaatacagc
      181 taaagaacca ggaatagcaa atacaaaagg actttaaaat tatgaaaatg agcttttaaa
      241 tagctcattt ttcttttaag gaatttagat taaagaatct ttggatataa aatataagat
      301 tttataagtt tttagagata ttaatattag ggttaattca taataaaaga ctattctatt
      361 aagtataatg gagatttact taataaatta taaattttag aggtatttaa ggattgtttt
      421 tctaaaaaga taaaaagtga taatcatttt caataaatgt gatttaaatc atatttttaa
      481 tatgaaaaat agattaaaat taaggcataa aatctattag aaaagtaaag aataaaaata
      541 attgagtatt aacttgaatt ttcttaagga ggaatttaaa gatgaaacaa tatattgaaa
      601 taattgacgt ggtagcaaga caaattttag attcaagatg cttcccaaca gtagaggttg
      661 aagtttattt agaagatgga acagttggaa gagctgcagt accttcagga gcttcaacag
      721 gtatatatga agctgttgaa ttaagagatg gagataaaga taaataccta ggaaaaggtg
      781 ttgaaaaggc agtagcaaat gttaatgata caattgcaga agaaataata ggattaaatg
      841 tattagatca agcttacata gataaaactc ttatagagtt agatggaact aaaaataaag
      901 gtaaattagg agctaacgca atcttaggag tttctttagc agtagctcaa gcagcagcta
      961 actatttagg aatgccttta taccaataca ttggaggagt aaatgctaaa gttttaccag
     1021 ttccaatgat gaacataata aacggaggaa gccatgctga taactcagta gatatacaag
     1081 agtttatgat tatgccagta ggctttgatt gctttgaaag agctgtaaga gcttgtgcag
     1141 aagtttacca tgcattaaag aaaactttaa attcaaaggg atactcaact ggtgttggtg
     1201 atgaaggtgg atttgctcca aacttaaaat caaatgcaga agcaatagaa gttatattag
     1261 aagctattga aaaagcaggt tatgagccag gaaaagaatt cttcatagca atagatgcag
     1321 cttcatctga gtactacaaa gatggaaaat atgttttaga acatgaagga aaaactttaa
     1381 cagctgctga aatggttgac ttctttgaag attgggttaa caaatatcca ataatatcaa
     1441 tcgaagatgg tatggctgaa gaagactggg aaggttggaa gttaatgact gaaagacttg
     1501 gtaaaaaagt tcaattagtt ggtgatgacc tatttgttac aaatacagaa agattaaaaa
     1561 ctggtataga aaaaggaatt gctaactcaa ttctaataaa acttaaccaa ataggaacat
     1621 taacagaaac attaaatgct atagaaatgg ctaacagagc aggatataca gctgttgttt
     1681 ctcatagatc aggagaaact gaagatacaa ctatagcaga cttagttgtt gcagttaatg
     1741 caggtcaaat aaaaactggt gctccagcta gaagtgaaag agtagctaaa tacaaccaat
     1801 taataagaat aaatgaagag ttaggtgaag tagctgaata cagaggaaga aatgcattct
     1861 tcaacctttc aaaataatta tattaaatag taaaaataaa agtttaatag ataaaaacct
     1921 ctaagttttc ttaggggttt tttattttgg aattttataa
//
LOCUS       DAEVGI010000256         1951 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEVGI010000256 DAEVGI010000000
VERSION     DAEVGI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.258"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            93..1430
                     /locus_tag="K8M77_002508"
     CDS             93..1430
                     /locus_tag="K8M77_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HBI7086940.1"
                     /translation="MKKLFTTSLLILSMIFLIGCGSKSESKNFGLSVFGLEKFSGLDK
                     PEDNIKINLNGKIQDLSLPIYLDKNRYLIPISEIIKNNNGKFKIEDDSLNIKFENKDI
                     KVNLKDNTWTNLSKEESEANKFKIDPIVKDDTVYMSLIDFVNIFDLKSRWNSKDKLIK
                     LYNNKDMLDVKPYKGKGPQKGIIRFEDVASTGTGTEYDSQYLETIRVMGRYLDKKNVP
                     YHIAWIPRYIDPEKKIDNDPSKENNFANAELVYTLDFVASHKGEIGLHGYTHQIGNTI
                     SGHGFEFGKYNPSVEDLKSRVSKALQIAKDLDIKINFFEAPHYTINKAQNEALEKNFK
                     YIFNDYDENKAQSKPMKSPTGSGSYYVPTPLYYIEGGKENDMLNKIKNMSNTTFAGMF
                     YHPFLEAKLIDFKDGQDGYPEDNYKKPSIIQKVIDEFEKRNVSIISIEQVSEK"
     gene            complement(1497..1913)
                     /locus_tag="K8M77_002509"
     CDS             complement(1497..1913)
                     /locus_tag="K8M77_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATPase"
                     /protein_id="HBI7086941.1"
                     /translation="MKGFFKDISYDDRNLLAIGLAFISNLIFLNITQRKVGFNNIYEI
                     LNLKGYEALKEVFVIFITFIAIIFLIFAMIISINIILHSLEVFNKIVSLGTIIVIFII
                     AFYGLNNSIQGFEILFLTLFFVGLFFKIWEEIKNNK"
BASE COUNT          818 a          237 c          237 g          659 t
ORIGIN      
        1 aaatttaaac cttaatttgt aaaatattat tataaatgta cactttttat aaatttatta
       61 atataattta aattaataca aggaggcact atatgaaaaa actttttaca acttcactct
      121 taattctttc aatgattttt ctaataggat gtggatctaa aagtgaaagt aaaaactttg
      181 ggctatctgt atttggatta gaaaagtttt ctggattaga taaacctgaa gataatatta
      241 aaataaattt aaatggtaaa attcaagact taagcttacc aatatactta gataaaaata
      301 gatatctaat ccctataagc gaaataatca aaaataacaa tgggaagttt aaaatagagg
      361 atgattcttt aaatattaaa tttgaaaata aagatattaa agttaattta aaagataata
      421 cttggactaa tttatcaaag gaagaatcag aggctaataa gtttaaaatt gatcctatag
      481 taaaagatga tactgtatat atgtctttaa ttgactttgt taatatattt gatttaaaaa
      541 gcagatggaa ctcaaaggac aagctaataa aattatataa taataaagat atgttagatg
      601 ttaaacctta taagggaaaa ggccctcaaa aaggtataat aagatttgaa gatgttgcat
      661 ctactggtac tggaacagaa tatgattcac aatatcttga aactataaga gttatgggaa
      721 gatatttaga taaaaaaaat gtaccttacc atatagcttg gatacctagg tatatagatc
      781 ctgaaaaaaa aatagacaat gacccttcta aagaaaataa ctttgctaat gctgagcttg
      841 tatacacttt agactttgta gcttctcata aaggggaaat aggcttacac ggatatactc
      901 atcaaatagg taatacaatt agtggccatg gctttgaatt tggaaaatac aatccatctg
      961 tggaggattt aaagtcaaga gtttctaaag ctcttcaaat agctaaagat cttgatataa
     1021 aaataaattt ctttgaagct cctcattaca ctataaataa agctcaaaat gaagctttag
     1081 aaaagaactt taaatatata tttaatgatt atgatgaaaa taaagcacaa tcaaagccta
     1141 tgaaatcacc aactggaagt ggttcttatt atgtacctac acctctatat tatattgagg
     1201 gcggtaaaga aaatgatatg ttaaataaga taaaaaacat gtctaatact acttttgctg
     1261 gaatgtttta tcatccattt ttagaagcta aactaataga ttttaaagat ggacaagatg
     1321 gttatcctga agataattat aaaaaaccat ctataatcca aaaagtaatt gatgaatttg
     1381 aaaaaagaaa tgtatctatt atttcaatag aacaagtttc tgaaaaataa atttcacttt
     1441 taaagaaagg ttaagttaaa attatttatt ttaacttacc ctttctttaa ttttaattat
     1501 ttattatttt ttatctcttc ccatatctta aaaaataatc ctacaaaaaa taaagttaaa
     1561 aataatatct caaatccttg aatactgtta tttaatccat aaaaagctat tataaaaatt
     1621 actataatag ttcctaagga tactatttta ttaaagactt ctaaggaatg taaaattata
     1681 tttatactaa taatcatagc aaaaatcaaa aaaattatag ctataaaagt tataaaaata
     1741 acaaatactt ccttcaaagc ttcatacccc tttaaattaa gtatttcata gatattatta
     1801 aatcccactt tcctttgtgt tatatttaaa aatattaaat ttgatataaa tgcaagacct
     1861 atagctaaaa gatttctatc atcataagat atatctttaa aaaacccctt caaagattcc
     1921 tcctaaaaaa tcactttact aaactatatt c
//
LOCUS       DAEVGI010000257         1938 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEVGI010000257 DAEVGI010000000
VERSION     DAEVGI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.259"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            90..938
                     /locus_tag="K8M77_002510"
     CDS             90..938
                     /locus_tag="K8M77_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBI7086942.1"
                     /translation="MIKKTEKAYEVKRSFNNNIVLVDDDKKEIILFAKGIGFNCKKGS
                     VIPENTNIEKIFTIQDENNIANFRRVIGSNDEKFIAFCEELILEISRDFNEELNEIIH
                     IGLIDHISFAIERMKKGEILRNPFLVEIETLYPKEFEAATKMVKKLEDFTGVSIVEDE
                     IGFIAMHIHSARTTGKISHTIKFTYISNTAVEYIEDAFNIVVDRTSLSYARFISHLRF
                     AIERLINNTPIKNELLSIIKLKYPESFKVSEEIGKAINEDFSLKVAEDEIGYLALHVE
                     RIRKNE"
     gene            complement(971..1450)
                     /locus_tag="K8M77_002511"
     CDS             complement(971..1450)
                     /locus_tag="K8M77_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBI7086943.1"
                     /translation="MFGLFKKELKLIAPATGKTIDLSQVPDQVFAEKMAGDGVAIEVT
                     GDTIVAPADGELSLVFKTNHAFAMTLSNGIELLVHVGIDTVSLEGEGFERLTEPGQQV
                     KAGTPILKIDRDFILGKGLKLHTPVLITNPDMVKDMKPVIGETVTSGNDTVLTYKVK"
BASE COUNT          740 a          215 c          291 g          692 t
ORIGIN      
        1 taagttactt ttttataaat atggaatgaa tttaaataag aattaatata aatttagtta
       61 gcagtaataa tggtatagag gagaatatta tgataaaaaa aacggaaaaa gcatatgagg
      121 taaaaagatc cttcaataat aatatagttt tagttgatga tgataaaaaa gaaattatat
      181 tatttgctaa aggtataggc tttaattgta aaaaaggttc agtaatacct gagaatacta
      241 atatagaaaa aatatttact atacaagatg agaataatat tgctaacttt agaagagtga
      301 ttggaagtaa tgatgagaaa tttattgctt tttgtgaaga attaatatta gagatttcaa
      361 gagattttaa tgaggaatta aatgagatta tacatatagg gctcatagac catatttctt
      421 ttgctattga aagaatgaaa aaaggagaga ttttaagaaa tcctttttta gtagaaatag
      481 aaactctata tcctaaagag tttgaggctg caactaaaat ggttaaaaag cttgaggatt
      541 ttacaggggt aagtatagtt gaggatgaga ttggatttat tgctatgcat atacattcgg
      601 caaggacaac aggaaagatt tctcatacaa taaaatttac ctatatatca aatactgctg
      661 tagaatatat tgaagatgct tttaatattg tggtggatag aacttcatta tcttatgcta
      721 gatttataag ccacttaaga tttgctattg aaagacttat aaataatact ccgataaaaa
      781 atgaattact ttctattata aaactaaaat atccagaatc ttttaaggtt tctgaagaaa
      841 taggaaaagc tataaatgaa gatttttctc tgaaagttgc agaagatgag atagggtatt
      901 tagctttaca tgtagagaga ataagaaaaa atgaataata taaaagctga cttaaaagtc
      961 agcttttata ttatttaact ttataagtta aaacagtatc atttccagaa gtaactgttt
     1021 cacctataac aggtttcata tcttttacca tatcaggatt tgtgattaat acaggagtgt
     1081 gtaattttaa gccttttcct aatataaaat ctctatctat ttttaatatt ggtgtaccag
     1141 ctttaacttg ttgtcctggt tcagttaatc tttcgaatcc ttctccttct agagaaactg
     1201 tatctatacc gacatgaact aataattcaa taccattact taaagtcata gcgaaagcat
     1261 ggttagtttt gaatactaat gataattctc catcagcagg agcaacaata gtatctccag
     1321 ttacttcaat agcaactcca tctccagcca ttttttcagc gaaaacttga tctggtactt
     1381 gtgataaatc aatagttttt ccagttgcag gagcaattaa ttttaattct tttttaaata
     1441 atccaaacat atttattttc tcaacttatt gtattaagtt aagaaattca catcctttct
     1501 atatatagaa tcaaatataa aaaaacaggc ataagtctat tagaacttat gcctgattta
     1561 acagtaacat gttatgatta cattatatat tctttatttt tataagtcaa cactatattt
     1621 atacaatatc atttgaaaat ttgtatttat atgtaataaa tttaggaagc ttgagaaaga
     1681 taatattatg acaatatagt tatcttaaag ttgacaccct aaaaaagata tagtaatatt
     1741 taaatgcaaa tattacagct ttaatgttta agatattatt ttaagcgtgt aaccataagt
     1801 tttgggcatt agctgaaaag aattactatg ttcttttcag ctttttttat gtgtgaatat
     1861 aattatatta atagaataat ttttagctaa aggggaaagg atttcaaaag gaatttctat
     1921 ataagtattt tatgatta
//
LOCUS       DAEVGI010000258         1924 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEVGI010000258 DAEVGI010000000
VERSION     DAEVGI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.260"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(92..784)
                     /locus_tag="K8M77_002512"
     CDS             complement(92..784)
                     /locus_tag="K8M77_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBI7086944.1"
                     /translation="MKKLDDNKLKEISFFSGLNDETLKYMEKNSKVLKKKKGEHIFRD
                     KDKIQDIFIVLKGKVTLYKMNEYGQKRIVYILGEESFLNEVIVDDLPASINGEVFENS
                     IIFSIDKNKLLHIMSKDFELTKIVLNEMSKKVRRLYRQMKNVTPIKLEKRVAAKLWKL
                     SKDYGKEVPGGTLIDLDISITYLADMFGSPRESVSRAIKTLRDENLIIKENKKIIIPD
                     RERIAAYFKSLI"
     gene            complement(995..1792)
                     /locus_tag="K8M77_002513"
     CDS             complement(995..1792)
                     /locus_tag="K8M77_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBI7086945.1"
                     /translation="MFKEEFNNVISAGRRKIDLLNNSKGRYLVYAMLAGLYVSFGIML
                     AFTIGGILDSANSPNTKIMMGASFGVALSLVVFAGSELFTGNNFVMTIGMLNKKITFK
                     ECWKIWGLAYIGNFLGSIIAALGFIVAGLTKGSVSDFMIKTAEIKISLPPEQLFIRGI
                     FCNILVCLAVWCTFRLKTETSKLIMIFWCLFAFVTSGFEHSIANMSLLVIGSILTKGA
                     TITVAGSLYNLFFVTLGNILGGVILGLAYYYVSNKKVENKKIVYIKK"
BASE COUNT          674 a          331 c          153 g          766 t
ORIGIN      
        1 tattgagtat gtgattttta tcacactgta aaaaattata aaactttaga ataaatagca
       61 aattttataa atttcaattt tcaaggtctt tttatattaa tgatttaaaa tatgcagcaa
      121 ttctttctct atcaggtatt ataatctttt tgttttcttt gattattaga ttttcatctc
      181 ttaaagtttt aatagctcta gatacacttt ccctcggaga accaaacata tctgctaagt
      241 aagttatgct tatatctaaa tcaataagtg taccccctgg tacttctttt ccataatcct
      301 tacttaactt ccaaagcttt gctgctactc ttttttctaa ttttattgga gttacatttt
      361 tcatttgtct atataatctt cttaccttct tgctcatttc atttaaaaca atctttgtaa
      421 gttcaaaatc tttactcatt atatgtaata acttattttt atctatacta aatattattg
      481 agttttcaaa aacttctcca tttattgaag ctggaaggtc atcaactatt acttcattta
      541 aaaaactttc ctctccaagt atataaacta ttctcttttg tccatattca ttcatcttat
      601 acaaagttac ttttcctttt aaaacaataa aaatatcctg aatcttatct ttatctctaa
      661 agatgtgctc tccctttttc ttttttaaga ctttagaatt tttctccata tattttaatg
      721 tttcatcatt aagtccagaa aagaaactta tctcttttaa tttattatca tctaattttt
      781 tcatactctc tcctactata caaaattctc attattttaa cgcaatataa tctaatttaa
      841 cataaaattt tattaaataa tataacttct atacttatat ttttaacata aaaaaaatat
      901 atataatagg cttttattaa aatgtaaaaa gcttacaaat attataaaga gggttatatt
      961 aattctcaaa atatatttgt aagcttttta aaaattattt tttaatataa actatctttt
     1021 tattttctac ttttttatta gatacatagt aatatgctaa acctaaaata acaccaccta
     1081 gtatattacc taatgtaaca aaaaatagat tatataaact tcccgctact gttatagtag
     1141 ctccctttgt taatatactt cctattacta ataagctcat atttgcaatg ctgtgttcaa
     1201 aaccagatgt tacaaatgca aaaaggcacc aaaatatcat gattaacttt gatgtttctg
     1261 tttttaatct aaaggtacac catacagcta aacatactaa tatgttacaa aatattcctc
     1321 ttatgaataa ctgctctggt ggcaagctta tttttatttc cgcagtttta atcataaaat
     1381 cacttacact acctttagtt aaacctgcaa ctataaaacc taaagcggct attatagatc
     1441 ctaaaaaatt accaatgtaa gctagtcccc atattttcca acattcttta aatgttattt
     1501 ttttatttag cattcctata gtcattacaa aattatttcc tgtaaaaagt tccgaccctg
     1561 caaaaaccac taaacttaaa gctacaccaa aagatgcccc catcattatt ttagtatttg
     1621 gagaatttgc actatctaat attcctccta tagtaaatgc taacattatt ccaaaactta
     1681 cataaagtcc tgcaagcata gcatatacta aataacgtcc cttactatta tttagcaagt
     1741 caatttttct cctacctgca ctaataacat tattaaattc ttccttaaac atgttaaagc
     1801 ctcctaactt aaaagtatcc tattagattc taaaataaaa agaccttaat cactatagta
     1861 aaagaaaaaa aattttattt ttaacctttt atccacattt aattaataat aaatcaatat
     1921 tttt
//
LOCUS       DAEVGI010000259         1911 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEVGI010000259 DAEVGI010000000
VERSION     DAEVGI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.261"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            46..122
                     /locus_tag="K8M77_002514"
     tRNA            46..122
                     /locus_tag="K8M77_002514"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Arg,seq:tcg)
     gene            303..1754
                     /locus_tag="K8M77_002515"
     CDS             303..1754
                     /locus_tag="K8M77_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBI7086946.1"
                     /translation="MRVLENLEPKSVFRFFEDLTRIPHDSGNEKELSDYLVKFAKDRN
                     LEVIQDEALNVIIKKPATKGYENVPGVIIQGHMDMVCEKLKSSNHDFKKDPLKLRIID
                     DKFVYATDTTLGADDGISLAYGLAILDSNNIEHPAIEFVATTEEETVMGGATALDTSL
                     LKGKVLLNIDAEEEGVFIVGCAGGIMVYPEINAEFEDFNGEALKLEISGFKGGHSGME
                     IHKQRGNANKLMGRILYALSKEVDFNIASIKGGSKHNAIPQYCQSIIAVKKEDREKVK
                     EICTALEKDLKAEYRIGEPDVNLSVKSIGGVEKQLTKKVTDDITRFLVLVPDGLQSMS
                     QEINGLVESSLNLGIVEMVEDKIKFIIDIRSAVKSKKIEITNRVEALCKVIGANMTKD
                     GDYPEWEYEAESKIKDLSIKTYSDLFGIEPKITALHAGLECGIFKEKMGKEVEIISFG
                     PDIFDVHTANEHFKIESVERCYRFLIELLKNMK"
BASE COUNT          777 a          175 c          367 g          592 t
ORIGIN      
        1 aaaagataac cgattcggtt atcttttttt attttaaaaa tataagcatc tatagctcag
       61 ctggatagag cgttggactt cgaatccaca ggtcgcgagt tcgaatcttg ctaggtgcac
      121 caattttaag ggttacaatt attttgtaac ccttattttt taggttaatt tttgaaaata
      181 attaattaaa tgatattata aaattatagg taatatataa tatttacaat atagataaaa
      241 ctaaattagt aattataaag tttaaattga ttattaatta tttagctaga ggaggaagta
      301 aaatgagagt attagaaaac ctagagccta aaagtgtatt tagatttttt gaagatttaa
      361 caagaatacc acatgattct ggaaatgaaa aagaacttag tgattatctt gttaagtttg
      421 caaaggatag aaatcttgaa gttattcagg atgaggcact aaatgtaata attaaaaagc
      481 ctgcgactaa ggggtatgaa aatgtaccag gagttataat tcaagggcat atggatatgg
      541 tatgtgaaaa attaaagagt agtaatcacg attttaaaaa agatccttta aaattaagaa
      601 ttattgatga taaatttgtt tatgctacag atactacttt aggagcagat gatggtattt
      661 cattagctta tggattagct attttagatt ctaacaatat agaacatcca gcaatagagt
      721 ttgtagcaac tacagaggaa gaaacagtta tggggggagc tactgcttta gatacttcac
      781 ttttaaaagg aaaggtttta ttaaacatag atgctgaaga agaaggagta tttattgtag
      841 ggtgtgctgg aggaattatg gtttatccag aaataaatgc tgaatttgaa gattttaatg
      901 gagaagcttt aaaattagag atttcaggtt ttaagggtgg acactcagga atggaaatcc
      961 ataaacaaag aggaaatgcc aataagttaa tgggaagaat cttatatgct ttaagcaaag
     1021 aagttgactt taatattgca tcaattaagg gtggatcaaa acataatgca ataccacaat
     1081 attgtcaaag cataattgca gttaagaaag aagatagaga aaaggttaaa gaaatttgta
     1141 ctgccttaga aaaagattta aaggcagaat acagaattgg agagccagat gtgaatcttt
     1201 ctgttaaaag cattggagga gtagaaaaac aattaactaa aaaggttaca gatgatataa
     1261 ctagattttt agttttagtt ccagatggat tacaatctat gagtcaagag ataaatggat
     1321 tagttgaaag tagcttaaat cttggaatag tagaaatggt agaggataaa attaaattta
     1381 ttattgatat aagaagtgca gttaagagta aaaagataga aatcacaaat agagtagagg
     1441 ctctttgtaa agttatagga gctaatatga ctaaagatgg agattatcca gagtgggaat
     1501 atgaagcaga atcaaaaata aaagatttaa gtattaaaac ttacagtgac ttatttggaa
     1561 ttgagcctaa aataacagct ttacatgcag ggcttgagtg tggaatcttt aaagaaaaga
     1621 tgggaaaaga agtggaaatt ataagctttg gtccagatat atttgatgtg catacagcaa
     1681 atgagcattt taaaatagaa tctgttgaaa gatgttatag attcttaatt gaattattaa
     1741 agaacatgaa ataaaaatta aaatttttat attaaaatta aactttcaat tgaatacgta
     1801 aatttaagag caaaaaataa aaaaaaggag gaaatgctac tagtcaaaat attaaaaact
     1861 aataattaaa acttaggtaa tgtaattatt agtttttatt aaaaaagaaa t
//
LOCUS       DAEVGI010000260         1907 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEVGI010000260 DAEVGI010000000
VERSION     DAEVGI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.262"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(35..226)
                     /locus_tag="K8M77_002516"
     CDS             complement(35..226)
                     /locus_tag="K8M77_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086947.1"
                     /translation="MGGLIVSYKNKINLIFIILVTISFLIYRFGFDRNGNLLSANYII
                     MYIIFLGLIISFIINLIKK"
     gene            complement(223..774)
                     /locus_tag="K8M77_002517"
     CDS             complement(223..774)
                     /locus_tag="K8M77_002517"
                     /inference="COORDINATES: protein motif:HMM:NF014052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBI7086948.1"
                     /translation="MSDSILRDIKRSFLLINIIYILFFLLGFFFSDYFKEIFNDGYIF
                     NPENIQIDSNKHIFVDIFNNNIRLGIKISFGVITLGLSSIFFIILNGFFFGIVSNFAI
                     NIYSLNYYLRLIVFHGIIEILTFNLLATATIIPLIFIKNKIKNKRFKISEYFKSYLFL
                     TFLGLVLILICALIESFVSTLML"
     gene            complement(767..1516)
                     /locus_tag="K8M77_002518"
     CDS             complement(767..1516)
                     /locus_tag="K8M77_002518"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI7086949.1"
                     /translation="MDSLENIIEVKEISKSFNNSNFKIENLSFELKKGQITGLVGKNG
                     AGKTTIIKILIGLLAPDSGSIKILNEDLKKNFGKVKNKIGYMHTEYHFYDYLTGREFL
                     QFIGEIKGMKNLLIEERIKTYSEYFELEDLLDSIIINYSHGTKQKLSFISQIINNPDI
                     LILDEPLNGLDPIMIKKMRELIKNLAINNDMCILVSAHQLSFIKEICNAIILVDNGRL
                     LLNKEILDSNLDLFENEILNLLEKNKELNYV"
BASE COUNT          762 a          248 c          137 g          760 t
ORIGIN      
        1 tcttatctaa cttactaaaa atatgattaa ttacttattt ttttattaaa ttaattataa
       61 atgatattat taaccctaaa aaaattatat acattattat ataatttgct gaaagcaaat
      121 taccatttct atcaaatcca aatctataaa taagaaatga tattgttact aatataataa
      181 atattaagtt aattttattt ttataactca ctattaaacc tcctataaca taagcgtaga
      241 cacaaatgat tcaatcaatg cacaaattaa aattaacact aaacctaaaa atgttaaaaa
      301 taaataactt ttaaaatatt ctgatatttt aaatctctta tttttaattt tattttttat
      361 aaatattaaa ggtattattg tagcagttgc taatagatta aaagttaata tctcaatgat
      421 tccatgaaaa actattaatc ttaaataata atttaacgaa taaatattta tagcaaaatt
      481 agaaacaatt ccaaaaaaga agccatttaa tattataaaa aaaatactac ttagtcctaa
      541 tgttattact ccaaatgaga ttttaatacc taatcttata ttattattaa atatatcaac
      601 gaatatatgc ttattactat ctatctgaat attttcagga ttaaaaatgt aaccatcatt
      661 aaatatctcc ttaaaatagt cactaaaaaa aaatcctaat aaaaaaaata atatatatat
      721 aatattaatt aataaaaaac ttctttttat atctcttaaa atactatcag acataattta
      781 attccttatt tttttctaaa agatttaaaa tctcattttc aaataaatct aaattagaat
      841 ctaaaatttc tttattcaac aacagccttc cattgtcaac caaaataatt gcattacaaa
      901 tctccttaat aaaagataat tgatgagcag aaactaatat gcacatatca ttatttattg
      961 ctaagttttt tattaattct ctcatttttt ttatcattat tggatctaat ccatttaagg
     1021 gctcgtctaa aattaatata tctggattat taattatttg actaataaat gataatttct
     1081 gttttgtacc atgtgaataa ttgattataa ttgaatccaa aagatcttct aattcaaaat
     1141 actcactata ggtcttaatt ctttcttcta ttaataaatt tttcatcccc ttaatttcac
     1201 caataaattg caaaaactct cttccagtta aataatcata aaaatgatat tctgtatgca
     1261 tataacctat tttattttta acttttccaa aatttttttt taaatcttca tttaaaattt
     1321 taattgaacc actatcagga gctaaaagac caattaaaat tttaataatt gttgtttttc
     1381 ctgcaccatt tttaccaact aatcctgtaa tttgaccttt ttttagttca aagcttaaat
     1441 tttctatttt gaaatttgag ttgttaaagg atttacttat ttctttaact tctattatat
     1501 tttctaaaga atccaatatg ctttctcctt tcttttataa taaaaaatca taatgtaaat
     1561 aaaaatagat gcaattatta aatttattaa tgatgtgctt aaaataaacc ctttttttaa
     1621 taagattcct ctatagttta gtatacccaa aattgaatta acactaattg ttaaccatac
     1681 gtatatactt agtataattt gagcaattaa atttcccttt gagtttattt tatcattact
     1741 gtattgctct ttattaaagt ttgggaaaat agcatcacta aaatattcta tacatacaac
     1801 tgtagaaata tatgaaatgc cccaaatatt acaatacata taatccaaaa ttgatttgga
     1861 taatattaag aatacattaa taggagttaa tataaggaaa tttatta
//
LOCUS       DAEVGI010000261         1801 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEVGI010000261 DAEVGI010000000
VERSION     DAEVGI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.263"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            153..863
                     /locus_tag="K8M77_002519"
     CDS             153..863
                     /locus_tag="K8M77_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoprotein"
                     /protein_id="HBI7086950.1"
                     /translation="MFKIEEEAKDIKSFYLRATDGKPLKEYKCGQFLPLKIETDDEVI
                     KKEMRRYSLSGDPKKDYYRLTIKRVPNGKVSGYFHESINVGDTILAMPPFGKFTLTED
                     SNKPLVLLSGGIGITPILSMLYGAKEQSRDVYFVEAVLNSDNLALNSDVQGIKKVKNF
                     KEIKIFSEPLESDIKGKDFDEEGFITKEWIKNNLPLNGEFFFCGPTLFMKSIRNSLKD
                     LGVKEDNINFEFFGKEEF"
     gene            1082..1792
                     /locus_tag="K8M77_002520"
     CDS             1082..1792
                     /locus_tag="K8M77_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBI7086951.1"
                     /translation="MIRLCNCASDKGPCARNIAIFSSLSDEELFKIVHMTEHKEFDEN
                     DTLCREGEISDKLFLIREGEVKICKITKEGKEQIVYLLSKGDFFGENNLFVNSNISNF
                     SAYAITSGKMCVLNKKSLDKILLENPEISIKIIEEMANRINLAENLAQNLATKDVEVR
                     IASVLVEFMNRYGIEKEEGIYVKLPLNREQMANYCGVTRETVSRKLSKFDKTGIINLH
                     GNKAIIIKDIEALLDLAE"
BASE COUNT          758 a          141 c          319 g          583 t
ORIGIN      
        1 attataggtt taactgtaag taacaatata tgaagtttta ttaagtatta atttaagtca
       61 tatattgtta cttatttaat aattataaac ttatgataag tgtaatgagg tgataatatg
      121 gaaatgtttg gaatggattt agacaattag aaatatttaa aatagaagaa gaggcaaagg
      181 atataaaatc attttaccta agagctactg atggtaaacc attaaaagaa tataaatgtg
      241 gtcaattttt acctcttaaa atagaaactg atgatgaagt tataaaaaaa gaaatgagaa
      301 gatatagttt atcaggtgat ccaaaaaagg attattatag attaacaata aagagggtgc
      361 ctaatggaaa ggtaagtgga tattttcatg aatctattaa tgtaggtgat acaatattag
      421 caatgcctcc atttgggaaa tttacattaa cagaagatag taataagcct ttagttttac
      481 tatcaggagg tataggaata acacctattt tatcaatgct atatggagct aaggaacaaa
      541 gcagagatgt atactttgtg gaggctgtat taaactcaga taatctagct ttaaattcag
      601 atgttcaagg aattaaaaaa gtaaagaatt ttaaggaaat aaagatattt agtgagccat
      661 tagaaagtga tataaaaggt aaagattttg atgaagaggg atttataaca aaagaatgga
      721 taaaaaataa cttgccttta aatggagaat ttttcttttg tggcccaact ttatttatga
      781 aaagtataag aaatagttta aaagatttag gcgttaaaga agataatatt aattttgaat
      841 tctttggaaa agaagagttt taataattat tttttagatt ttaatataat aacacttagt
      901 atattaaaaa tttggattta aaatgttata aaaagtagtt aattcttaag aattaactac
      961 ttttgttttt aataatatta tatgcctata tttttaaaaa cttatgatat attgatagtt
     1021 attaatagca tttgttaaaa ttgatatgaa ataccaaaag ataaaaggag cgtataaatt
     1081 tgtgattaga ttatgtaatt gtgcatcaga taaaggacca tgtgctagga atatagctat
     1141 tttctcatca ttatcagatg aggaactatt caaaatagtc catatgacag aacataagga
     1201 atttgatgaa aatgatacat tatgtaggga aggtgaaata tcagacaagc tttttttaat
     1261 tagagaggga gaagtgaaaa tatgtaaaat tactaaagaa ggaaaagaac aaatagtata
     1321 tctattatcc aaaggagatt tctttggaga aaataatctt tttgttaata gtaatattag
     1381 taatttttca gcttatgcaa taacttcagg taagatgtgt gtattaaata aaaaaagttt
     1441 agataaaata cttttagaaa atccagaaat atcaataaaa ataatagaag agatggctaa
     1501 tagaataaac ttagcagaga atttagcaca aaatttagca actaaggatg tagaagtgag
     1561 aatagcatca gtgttggtag aatttatgaa tagatatgga attgaaaagg aagaaggaat
     1621 atatgtaaag ttaccactta atagagaaca aatggcaaac tactgtggtg ttactagaga
     1681 aactgtaagt aggaaactat ctaaatttga taaaacagga ataataaatt tacatggaaa
     1741 taaggcaatt ataattaagg atatagaagc tttattagat ttagcagagt aaggtaaaag
     1801 g
//
LOCUS       DAEVGI010000262         1793 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.264, whole genome shotgun sequence.
ACCESSION   DAEVGI010000262 DAEVGI010000000
VERSION     DAEVGI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.264"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(96..1709)
                     /locus_tag="K8M77_002521"
     CDS             complement(96..1709)
                     /locus_tag="K8M77_002521"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBI7086952.1"
                     /translation="MKIPCSMMTQHPDNVETYISIQQEPAEAIKGLTPQDKGGLGIEE
                     VMIDFEGKLTPYHQTSQIALGLISNGIIPGKDVRVTPRIPNANKESVFRQLMSIMSII
                     ETNVQSKELTGTLAISEVVVPMIETGKEISEFQDRVNSVVDMGNKNYKTKLDLNSVRI
                     IPLVEDVPALVNIDRILDEHYQIEKSKGHVLKDLRIMIARSDTAMSYGLISGVLSVLM
                     AVDGAYKWGEKHGVTISPILGCGSLPFRGHFSEENIDEILATYSGIKTFTFQSALRYD
                     HGEEATKHTVNELKAKIEESKPRNFSEEDKDLMKEFIGICSKHYLQTFLKVIDTVSFV
                     SDFIPKNRDRLTKAKTGLEYNREVANLDNVADLVKDEVLKQEILSIDNSKEYAVPRAI
                     SFTGAMYTLGMPPELMGMGRALNEIKTKYGQEGIDKLLEIYPILRKDLAFAARFANGG
                     VSKKIIDEEARQEYKEDMKYVNEILNLGLDYDFLNENEFYHTLLKTTKPIIMHLMGLE
                     ENVMRNSTEELKILNEWIVRMGKVRGSIG"
BASE COUNT          526 a          315 c          214 g          738 t
ORIGIN      
        1 caatattatt tattggcttc aaatcatgtt tattggcttt ttgttatagt taaatctaat
       61 agaatttcat aactctaact tatcaaaaac atattttatc ctatacttcc tcttacttta
      121 cccattctta ctatccactc atttaggatt ttaagttctt ctgtgctgtt acgcattaca
      181 ttttcctcaa gtcccataag gtgcataatt ataggctttg tagttttaag taaagtatga
      241 tagaattcat tttcgtttaa gaaatcataa tctaatccaa ggttaagaat ttcatttaca
      301 tatttcatgt cttctttata ttcttgtcta gcctcttcat ctattatctt cttagaaact
      361 cctccatttg caaatctagc agcaaatgct aagtcttttc ttaagattgg gtatatttct
      421 aaaagtttat ctataccttc ttgaccatac ttagttttta tttcatttaa tgctctaccc
      481 attcccataa gttcaggtgg cattcctaat gtgtacatgg ccccagtaaa tgatatagct
      541 cttggaactg catattcttt tgagttatct atgctaagaa tttcttgttt taatacttca
      601 tccttaacta aatctgctac attgtctaag tttgctactt ctctattata ttctaatcca
      661 gttttagctt ttgttaatct atctctattc ttaggaataa agtcagatac aaatgaaact
      721 gtatctatta cctttaagaa tgtttgaaga taatgttttg aacagattcc tatgaattct
      781 ttcattaaat ctttatcttc ttcactaaag tttctaggtt ttgattcctc aatttttgct
      841 tttaattcat taactgtatg tttagttgct tcttcaccat gatcatatct taaagcagat
      901 tgaaatgtga aagttttaat tccactatat gtagcaagga tttcatctat gttttcttct
      961 gagaaatgtc ctctaaatgg aagtgatcca caacctaaga ttgggcttat agttacacca
     1021 tgtttttctc cccatttata agctccatca actgccataa gaactgataa aactcctgaa
     1081 attaatccgt aagacatagc agtatcagat cttgctatca ttattctaag atcttttaaa
     1141 acatgtcctt tactcttttc tatttgataa tgttcatcta atattctatc tatatttact
     1201 agtgcaggta catcctcaac tagaggtatt attctaacac tatttaaatc taatttagtt
     1261 ttgtagtttt tattccccat atcaactact gagttaactc tatcttgaaa ctctgaaatt
     1321 tctttaccag tttcaatcat aggaacaaca acctctgaaa tagctagagt tccagttaat
     1381 tcttttgatt gaacatttgt ttcaattata gacattatac tcataagttg tctaaatact
     1441 gattctttat ttgcattagg tattctagga gttactctaa cgtcttttcc tggaattata
     1501 ccatttgaaa ttaatcctaa tgctatttgt gatgtttgat ggtaaggtgt taactttcct
     1561 tcaaaatcta tcataacttc ttctattcct aaaccacctt tatcttgtgg tgttaatcct
     1621 tttattgctt ctgctggttc ttgttgtatt gaaatataag tttctacatt atccggatgt
     1681 tgagtcatca tagaacaagg tatcttcata ataagtcccc cttttcgttt aaaaaatgaa
     1741 atttacaagt taattatatt gcaacaattt attaaaatca agtattttta aaa
//
LOCUS       DAEVGI010000263         1764 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEVGI010000263 DAEVGI010000000
VERSION     DAEVGI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.265"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            304..1695
                     /locus_tag="K8M77_002522"
     CDS             304..1695
                     /locus_tag="K8M77_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBI7086953.1"
                     /translation="MAEAIKSTDFEVEKGNGRLKHPAGLYLLFFTEMWERFSYYGMRA
                     LLMLYLTASFVTGGLGFDVPSAARIYGAYTFLVYFTPIIGGEIADKFLGQKKSIMLGA
                     AVMILGNLTLFGWQTRWALYLGLGFIIVGNAFFKPNISTIVGQLYEDGDKRKDSAFTI
                     FYMGINLGSLIAPIICGLLAENFFATKSGEVILHYGFRYGFLAAGIGIILGEIIFITL
                     SPKYLAHVGEIKREKNKKEVKKEKKPLTKQEKKRVAVILILASFVVFFWAGFEQAGTT
                     LTLFAEHATNRSLFGWTVPVAFFQSINPLFILILAPLFSKLWFTLANSKRGDLSIPTK
                     MAMGMIVLGCGFLLMVFATMSLGGNVENPAVKASMFWLVGTYLFNTMGELCLSPIGLS
                     MVSSLAPVKYASLLMGVWLASNGVANYLSGFIASFVEKLGALELFGSIAGVSIVLGLV
                     LLLLSKKITAMME"
BASE COUNT          661 a          185 c          318 g          600 t
ORIGIN      
        1 aaaaatagga tatgatttga taaaatgtta aaatgttgtc tatagatata aaagttttaa
       61 ttatatgttt ttctataaaa aacaattgtg ttgttatgat acacatagaa aaatggagta
      121 ttttagagga aatacaatta aactattaag aaacttgcaa taatttatta atagtaggtt
      181 tgattagaat taaaaatata tttataatta aaaatgtgtt aacaaaataa taagtttaat
      241 gataaattca tataaatcga tcatttgagt aaagtcataa ttaaaagagg gggatcaaca
      301 taaatggcag aggcaattaa atctactgat tttgaggtag aaaaaggtaa tggaagatta
      361 aagcatccag caggattata tcttttattc tttacagaga tgtgggaaag atttagttat
      421 tatggaatga gagctttatt aatgctttat ttaacagcat catttgttac tggtggatta
      481 ggatttgatg tacctagtgc agctagaata tatggagctt atactttctt agtttatttt
      541 acgccaataa ttggtggaga aatagcagat aaatttttag gtcagaaaaa atcaattatg
      601 cttggggcag cagtaatgat tttaggtaat ttaactttat ttggatggca aactagatgg
      661 gctttatact taggattagg gttcataata gtaggtaatg ctttctttaa accaaatata
      721 tcaacaatag ttggtcaatt atatgaagat ggtgacaaga gaaaagattc agctttcaca
      781 atattctaca tgggaataaa tttaggatct ttaatagcac caataatatg tggattatta
      841 gcagaaaact tttttgctac aaaatcaggt gaagtaatac ttcattatgg atttagatat
      901 ggattcttag cagcaggtat aggtataata ttaggagaaa ttatatttat aactttatct
      961 ccaaaatact tagcgcatgt tggtgaaatt aaaagagaaa aaaataaaaa agaagttaaa
     1021 aaagaaaaga aacctttaac taagcaagag aagaagagag ttgcagttat attaatactt
     1081 gcatcattcg ttgtattctt ctgggcagga tttgaacaag caggtacaac tttaacatta
     1141 tttgcagaac atgcaactaa tagaagttta tttggatgga cagtaccagt agcctttttc
     1201 caatctataa acccattatt tatactaata ttagcaccat tattctcaaa attatggttt
     1261 acacttgcta atagtaaaag aggagattta tcaatcccaa ctaaaatggc tatgggtatg
     1321 atagttttag gatgtggatt cttattaatg gttttcgcaa ctatgtcatt aggtggaaat
     1381 gttgaaaatc cagcagttaa agctagtatg ttctggttag taggaactta cttatttaac
     1441 acaatgggag aactttgttt atcaccaata ggactttcaa tggtaagttc attagcacca
     1501 gttaaatatg cttcattatt aatgggggta tggcttgcta gtaacggagt agcaaactac
     1561 ttatcaggat tcatagcatc atttgttgaa aaactaggag ctttagaact atttggttca
     1621 atagctggag taagtatagt attaggttta gtactattac tattaagcaa aaagattaca
     1681 gctatgatgg aataaaatga tattaaaatt aaatctcttt aagaaaattt gagtaattaa
     1741 aataggatat ttaagtttta tata
//
LOCUS       DAEVGI010000264         1764 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEVGI010000264 DAEVGI010000000
VERSION     DAEVGI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.266"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(752..1678)
                     /locus_tag="K8M77_002523"
     CDS             complement(752..1678)
                     /locus_tag="K8M77_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI7086954.1"
                     /translation="MKPIFKVSLYFLIVLVLSIVGPMFVTPVLYKIGLVPPLVLMVIH
                     IIFFIVPAIIYIIVTKSNYKKVFSFKKPKGKDVFFSILIAALALPIMTFFSYTSSFFY
                     TNDVALVLDQMRVYPLWLMILVVGVTPAITEEITIRGIALSGFEFKSKNVAAIMTGIM
                     FGILHLNAHQFLYATAMGIILAYVVRATGSIFLSMLIHFLINSWNLIQQRIVSQGITT
                     EDLVHSMDSIKNIPMELKIGVFFYYLIFAIVAAFLISYLIRKMEKRNFDITLDELDVA
                     TRVSYKEERVINVPFIISVIVFIVYTFFIMKG"
BASE COUNT          665 a          258 c          190 g          651 t
ORIGIN      
        1 caaatatata aaaaaatcat aacaatattt cacttttttt atttccttaa aattacacac
       61 tatagctcca tttttaatat gcgtatataa tttaataaat aaagttaatt tatatattaa
      121 gttcactaaa atttataagg aggtttttat tttgaaggtt aaaatacatc caaacacttt
      181 agataaagtt aaaaatatgc ttgataatag tgataaagat gcccttagaa taaaagcttg
      241 ttcctctgga tgagcaggct taaatattga agttgttctg gatgaacagc gtgaaaatga
      301 tgatttggtt atggataatg gtattaaaat agttgcagac aagaaaattt ctcatttctt
      361 tacagatgca accttagaat ataaaaacac tattctaggc aaaaaattta aaatatatta
      421 attggtatat ttttttaatt atcagtcaat aatactaata gcttaataca ctcttaagtt
      481 aaccacacaa tttaatagaa aaaggaactg ttatcccaaa cagttccttt tttcattctg
      541 tagacaaaat agtttttaat tactataaaa gctattttgt cttttttata tatacctttt
      601 aaaagtttca tattataaaa tttgcataag caaatttata atcggaatca aaaaggaatt
      661 cttattcctt ttccaaagct tatatgctat ttttttaaac tcatacatgc aaaaataagc
      721 caggtttcaa ataatattac aacgactttt attacccctt cattataaag aaggtatata
      781 caataaatac tattacactt attataaatg gtacatttat tactctctct tctttataag
      841 aaactcttgt tgcaacatct aactcatcta aagttatatc gaagtttctc ttttccatct
      901 ttcttattag gtaactaatt aaaaatgcag ctactattgc aaaaattaaa tagtagaaaa
      961 acacccctat ttttaattcc attggaatat tttttataga gtccatagaa tgtactaaat
     1021 cctctgtagt tattccttga ctaactattc tttgctgaat taaattccat gaattaatta
     1081 gaaaatggat taacattgat aaaaatatac ttcctgtagc tcttactaca taagctaaaa
     1141 ttattcccat ggctgtagca tataaaaatt gatgtgcatt taaatgaaga ataccaaaca
     1201 ttattcctgt cattattgca gctacatttt tacttttaaa ttcaaatcct gataaggcaa
     1261 tacctcttat agttatttct tctgttatgg caggtgttac ccctacaact aaaatcatta
     1321 accataatgg atatactctc atttgatcta aaactaaggc tacatcattt gtataaaaga
     1381 aacttgatgt atatgagaag aatgtcatta ttggaagcgc taaagctgct attaatatag
     1441 aaaaaaatac atcttttccc tttggttttt taaagctaaa aaccttttta taattactct
     1501 tagttacaat tatatatatt attgctggca ctatgaaaaa tattatgtgt attaccatta
     1561 aaactaatgg tggcactaat cctatcttat atagcactgg cgtcacaaac attggcccta
     1621 ctatagataa aactaatact ataagaaaat ataaacttac tttaaatata ggcttcatca
     1681 tatttctccc ttctataaag aattaattta aaaattacat aaatccaagt ttaatatttt
     1741 aaagcataat taatcatttt aaaa
//
LOCUS       DAEVGI010000265         1735 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEVGI010000265 DAEVGI010000000
VERSION     DAEVGI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.267"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1464
                     /locus_tag="K8M77_002524"
     CDS             <1..1464
                     /locus_tag="K8M77_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBI7086955.1"
                     /translation="RFSHRASILIGLMLIMTSKEVFFKVKRAYYVTLTLLIVGGAFAF
                     IKDLDYKEGIFILGVIILLILSKKSFYRKSIPIKVTKLSGILIVLSIVMIIFASFIHK
                     FNIHFSKNYKYYIDFFHSTKGYLRIALFTYISFIIFVIIWYLTMPKIEDDERYMDADL
                     EKVSKFFKEIDYGTIFSHLVYLKDKKVFWANEGESLIMYSKYKDKIIVLGDPIATKEN
                     LYSCIEEFQAFTNLYGYDVVFYEIEEKNFSTYHDAGYYFFKLGEEARIDLEEFNLIGS
                     KKSAFRNTLRRVEREGYNFSIIEPPFNNEVVSQLKEISDKWLGDRKEKGFSLGWFSED
                     YIQRSPIAILKNEEENKIMGFVTIMDANDGGETVAIDLMRIDKDAPNASMDYLMLNLF
                     LTFKEKGYKYFSLGEAPLSNVGFNTHSHLQEKLARLVYNSGNIFYSFDGLRRYKSKFS
                     PIWQPRYLAYPKFMSLPEVFINLCLLIVNSKERVEKK"
BASE COUNT          666 a          162 c          297 g          610 t
ORIGIN      
        1 aggttttcac atagagcttc aatattaatt ggattaatgt taataatgac ctctaaggaa
       61 gttttcttta aggtaaaaag agcttattat gttacattaa cattgcttat agtaggagga
      121 gcctttgcct ttataaaaga tttagattac aaagaaggaa tttttatttt aggagtaata
      181 atacttctaa tattatcaaa aaagagtttt tacagaaaaa gtattcctat taaggttact
      241 aaattaagtg ggatattaat agttctttca attgtaatga ttatctttgc gagttttata
      301 cataaattta acatacattt tagcaagaac tataaatact atatagactt tttccatagc
      361 acaaaggggt atttaagaat agcattattc acatatatat cctttataat atttgtgata
      421 atatggtatt taacaatgcc taaaatagaa gatgacgaaa ggtatatgga tgctgattta
      481 gaaaaggtat caaaattctt taaagaaata gattatggaa caatattctc ccatttagtt
      541 tatttaaagg ataaaaaggt cttttgggct aatgaaggag agtccttaat aatgtatagc
      601 aagtacaaag ataagataat agttttagga gatcctatag ctactaagga aaacctatat
      661 agttgtatag aagagtttca agcttttaca aatttatatg gatatgatgt tgtcttttat
      721 gaaatagaag aaaaaaactt ttctacctat catgatgcag ggtattattt ctttaagtta
      781 ggagaagagg caaggataga tttagaagaa tttaatttga ttggttctaa aaagagtgcc
      841 tttagaaaca ccttaagaag agttgaaagg gaaggatata attttagcat tatagagcct
      901 ccttttaata atgaggtagt aagtcaattg aaggaaatat ctgataaatg gttaggggac
      961 agaaaagaaa agggattttc tttaggatgg tttagtgagg attatataca aagatcacct
     1021 atagctattt taaagaatga agaagaaaat aagattatgg gctttgtaac aataatggat
     1081 gctaatgatg gaggggagac agtagcaata gatttaatga gaatagataa agatgctcca
     1141 aatgcctcta tggattacct aatgcttaat ttattcttaa cctttaaaga aaaaggatat
     1201 aagtatttta gcttaggaga agcaccatta tctaatgtag gatttaacac tcattcacat
     1261 ttacaagaaa agcttgcaag attagtttat aatagtggta atatattcta tagttttgat
     1321 ggactaagaa gatataagtc aaagttttct ccaatttggc aacctagata tttagcatat
     1381 cctaagttta tgtccttacc agaggtgttt attaacttat gtttattaat agttaattca
     1441 aaggaaagag tagagaaaaa ataaaaacgt atattttaag ttaagtttga tttaaaatta
     1501 aatttttata tctagcttaa ttaagtttat tatattaaag agaatctttt agttataagg
     1561 ctctcttttt ataaaaattt actttttata aataaagcct attttttata ttgaagttta
     1621 aaaaaatagg ggtattattt taaatttatt tagaagaaac aatgagtttt taaatagata
     1681 taaaaaatct aaggatttag gctaagattt gtcaaatgaa aatgttttga agtga
//
LOCUS       DAEVGI010000266         1734 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEVGI010000266 DAEVGI010000000
VERSION     DAEVGI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.268"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            14..1009
                     /locus_tag="K8M77_002525"
     CDS             14..1009
                     /locus_tag="K8M77_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBI7086956.1"
                     /translation="MNYIFLIIGFLLLIKGADLFVDGASGIAKKLGVPAVIIGLTIVS
                     LGTSAPELAVSISAALKGSNDITMGNVLGSNLFNLLAALGCTAIVAPLVIKKHIIKND
                     FIVNLLATFVLYVFTFTGLIGSKNSLSRMDGLVLVLMCITYIIYLIYTVKRSHKKEDE
                     VAVTVEFEDFEEKNIKSPNSTGKNILLSIVGVIGIILGGKIVVDSASAIALGLGLSEK
                     LVGLTIVAIGTSLPELVTSLVAAKKGENDIALGNILGSNTFNILLILGLSSLISPITI
                     AASLSVDLIFLIVVTLIIGALIFLNKKKEKVLTRYEGLFLLVLYVGYTVYIIMRN"
     gene            1148..1708
                     /locus_tag="K8M77_002526"
     CDS             1148..1708
                     /locus_tag="K8M77_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBI7086957.1"
                     /translation="MNSLINIFNQYSELAILISLFISIIIALLGIVPSVFITGANIVF
                     FGPINGFLISLLGEVIGGWISFKVYRKGVNKFAGNIEGKYELIDKIVKSEGKDIGILI
                     FEGRLIPFIPSGLVTLAAAISRVNSFTFIISTLLGKIPSILLEVLASYGVIIASQKNL
                     KLVIGVLSLILFLLTLKKLKEKMKKK"
BASE COUNT          640 a          173 c          298 g          623 t
ORIGIN      
        1 tggagaagaa ttaatgaact acatatttct aataataggg ttccttttac ttattaaagg
       61 agcagatctc tttgttgatg gggcttcagg aatagctaaa aagctaggag ttccagcggt
      121 tataattgga cttactattg tatcattagg aactagtgcg ccagagcttg cagtaagtat
      181 atcagctgca ttaaaaggta gcaatgatat taccatggga aatgttttag ggtcaaattt
      241 atttaacctt ttagcagctt taggatgtac agccattgta gctcctttag tcataaagaa
      301 gcacataata aaaaatgatt ttatagtaaa tttacttgca acttttgttt tgtatgtttt
      361 tacttttact ggactaatag gaagtaaaaa ttctttatct agaatggatg gattagtatt
      421 agtcttaatg tgtataacat atataatata tttaatatat acagttaaga gaagccataa
      481 aaaagaagat gaagtagcag taactgtaga atttgaagat tttgaagaga aaaacataaa
      541 gtcacctaat agcacaggca aaaatattct cttatcgata gttggagtta taggaataat
      601 attaggtgga aaaatagttg ttgattcggc ctctgcaatt gccttaggtt taggactaag
      661 tgagaaatta gttggactta caattgtggc aataggtact tctcttccag aacttgttac
      721 ttctttagtg gcggctaaaa agggtgaaaa tgacatagct ctcggaaata tattaggatc
      781 taatacattt aacatattat taatattagg tttatcatct ttaataagcc ctataacaat
      841 agctgcaagc ttatcagtag atttaatatt ccttatagtt gttactttaa taataggtgc
      901 tttaattttc ttaaataaga aaaaagaaaa agtacttact agatacgaag ggttatttct
      961 tttagttctt tatgtcggat atacagtata tataataatg agaaattaat tttcttactg
     1021 tcttaaatta agacagttat tttttatgtg aaatttagcg tatagtgtac acctttattt
     1081 aaaatttaat atattgggag gtatacttaa aataaaaatt aatatataga aaaaggagga
     1141 ggacaatata aatagtttaa taaatatttt taatcaatat agtgagttag ctatattaat
     1201 aagtttattt ataagtataa ttatagcatt acttggcata gttccctctg tattcattac
     1261 aggagctaat atagtatttt ttggtcctat aaatggattt ttaatttctt tattaggtga
     1321 agttataggg gggtggataa gttttaaagt atatagaaaa ggtgtaaata aatttgcagg
     1381 aaatattgaa ggaaagtatg aattaataga taaaattgta aagagtgaag gaaaagatat
     1441 tgggatttta atttttgaag gaagattaat accttttatt ccatcaggct tagtaacctt
     1501 agctgccgct ataagtagag ttaatagttt tacatttata atatctacat tattagggaa
     1561 aataccttct attttactag aagttttagc tagttatgga gtgataattg cttctcaaaa
     1621 aaatttaaaa cttgttattg gagttttgag tttaatttta tttttattaa cattaaaaaa
     1681 attaaaagaa aaaatgaaga aaaaatgata aatttatcat taaaaacaaa aaaa
//
LOCUS       DAEVGI010000267         1704 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEVGI010000267 DAEVGI010000000
VERSION     DAEVGI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.269"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            61..1404
                     /gene="miaB"
                     /locus_tag="K8M77_002527"
     CDS             61..1404
                     /gene="miaB"
                     /locus_tag="K8M77_002527"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011592162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBI7086958.1"
                     /translation="MTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENK
                     EEASIIIFNTCCVRENAENKVFGNLGQLKQLKKKNPNLVIAICGCMMQQVGMADKVLK
                     TFPYVDIIFGTHNAHKFPEYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVT
                     IMYGCNNFCTYCIVPYVRGRERSRKSEDIIKEIEELVSKGYKEITLLGQNVNSYGKGL
                     EEDIDFAGLLRKVNEVKGLERVRFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGS
                     SRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDIIIGFPGETEEDFLDTLSLCEEV
                     GYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLVEAINKKVVIKNKEYEGKVVE
                     VLVEGPSKNDETKLTGRTRNGKLVNFAGDEKLVGELVNLKIVRAQPFSLIGEIVE"
BASE COUNT          696 a          176 c          296 g          536 t
ORIGIN      
        1 aataaaaata aattttatgc tataatacaa cagataacaa tttaaattgg aggaaatgac
       61 atgacattag aaaataacat ggataaaaaa ctcttctgta tttctacata cggatgccaa
      121 atgaatgaag aagattcaga aaaattatca gggatgttaa aatctcaagg atatgaaaga
      181 actgaaaata aagaagaggc ttctattata atctttaata catgttgtgt aagagaaaat
      241 gctgaaaata aggtttttgg taatcttgga caacttaagc aattaaaaaa gaaaaatcct
      301 aacttagtaa tagcgatttg tggatgtatg atgcaacaag taggtatggc agataaagtc
      361 ttaaaaactt tcccttatgt tgacataata tttggaacac ataatgccca taaattccct
      421 gaatatctac atagagtact tcaagaaggt gttcaagtta aggaaatatt aaataaagaa
      481 gaaggaattg ttgaaggatt acctatagat agaaagagtg atgttaaagc attcgttact
      541 atcatgtatg gatgtaacaa tttctgtaca tactgtatag ttccttatgt tagaggaaga
      601 gaaagaagta gaaaatctga ggatatcata aaagaaatag aagaattagt atctaaagga
      661 tataaagaaa taactttatt aggacaaaac gtaaactcat acggaaaagg cttagaagaa
      721 gatatagact ttgccggtct tttaagaaaa gttaacgaag ttaaggggtt agagagagtt
      781 agatttatga cttctcatcc taaagattta agtgatgatg ttatcatggc tataaaagaa
      841 tgtgataagc tttgtgagca agttcactta ccagttcaaa gtggatcatc aagaatatta
      901 aaagagatga atagacacta tgacagagag tattacttag atttagttaa gaaaataaaa
      961 tcagaaatac cagatgttac tttaacaact gatataataa ttgggttccc aggagaaact
     1021 gaagaggatt tcttagatac attaagtctt tgtgaagaag ttggatatga ttcagcattt
     1081 acatttatct attcaagaag aaatcatact cctgctgaca aaatggaaaa tcaaattcca
     1141 gatgacataa agcatgatag attcaataga ttagttgaag ctatcaataa aaaggttgta
     1201 ataaaaaata aagaatatga aggtaaggta gttgaagttc ttgttgaagg accaagtaag
     1261 aatgatgaaa ctaaattaac tggaagaact agaaatggta aattagttaa ctttgctggt
     1321 gatgaaaaac tagtaggtga attagttaac ttaaaaatag ttagagcaca accattctca
     1381 ttaattggtg aaatagtaga ataatttaaa agtgggatgt atcaagattt ttgatacatc
     1441 ccacttaatt tttataaatt tgcaagtaat ctttcttgtt taattagggt atatttttat
     1501 tttagcttat ctagcaatat ctttttaaag tttaaaaagg tttcttaggc aatattttgc
     1561 tcacaaaaat atagctttta ataaattaat ataaattatt tgggaataat atagtttgaa
     1621 ataatgaaat tattctttat tacgaattat attcactatt tattaaatta aaattcgtta
     1681 atatgaaaaa aatttctatt gaac
//
LOCUS       DAEVGI010000268         1679 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEVGI010000268 DAEVGI010000000
VERSION     DAEVGI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.270"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            173..1630
                     /gene="gadC"
                     /locus_tag="K8M77_002528"
     CDS             173..1630
                     /gene="gadC"
                     /locus_tag="K8M77_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBI7086959.1"
                     /translation="MKQEGTTKKSLTLFGFFAMTASMVMAVYEYPTFATSKLHLVFYL
                     LLGGFLWFIPVALCAAEMATVDGWEKGGVFTWTGKNLGKKYGFANLFFEFFEITVGFV
                     TMIYFILGALSYVFDWPALNSNPMIKFIGVLVIFWILALSQFGGTKYTAKIAKIGFIV
                     GILLPAAILIILALVYIIQGNPIYISSKDTFVPDFTKVNTLVVFVSFILSYMGVEASA
                     THANEMDNPKKEYPLAMLLLVIVAIVVSTLGGLAIAAVIPQNQINLSAGVVQTFVVLL
                     GHFTANNTILVKIIALLLAFGVIAEVSSWVVGPTRGMIIAAEEGAIPKSWAKTNEHDV
                     PVYLVIAQGIIVSIWDAVLTFGAGGSNLSFLAAMSLTVVIYLSGYILFFVGYIKAILG
                     EGLNGAYQMPGGKPVKIIVAIIGLATSIFAFFISFVPPTSIAGNAVQSHEYMLMLIIS
                     YVISLILPFAIYDLCQKYMHSHEGNIHHIVVGHKH"
BASE COUNT          577 a          222 c          289 g          591 t
ORIGIN      
        1 aaacatttaa caaataagga gtaaagagtt ataatatata taaacaaaac gttaataaaa
       61 tgttaactgt gtttataact caaaaattta aaagtttctt acttctattt gaagcaacta
      121 actttttaat ttatttgagc taatcaaata acttaaaagg agttgaatta aaatgaaaca
      181 ggaaggaaca acaaaaaaat cattgacact ttttggattc ttcgcaatga cagcctctat
      241 ggttatggct gtatatgaat atcctacttt tgcaacatct aaattacact tagtatttta
      301 tctactttta ggaggtttct tatggtttat tccagtagct ttatgtgctg ctgaaatggc
      361 aactgtagat ggctgggaaa aaggtggagt tttcacttgg actggaaaga atttaggtaa
      421 aaaatatggt tttgcaaact tattttttga attctttgaa ataacagttg gatttgtaac
      481 catgatttat ttcatccttg gggctttatc atatgtattt gactggccag cactaaatag
      541 taacccaatg ataaaattta taggtgtatt agtaatcttt tggattttag cattatccca
      601 atttggagga acaaaatata ctgctaaaat agctaaaata ggttttatag taggaatact
      661 tttaccagca gctattttaa taatattagc tcttgtatat ataattcaag gaaaccctat
      721 atatatatca agtaaagata cttttgttcc agactttaca aaggtaaata ccttagttgt
      781 atttgtatcc tttatattat cttacatggg tgtagaagca tcagctactc atgcaaatga
      841 aatggataat ccaaagaaag aatatccatt agcaatgctt ttattagtta tagtagcaat
      901 tgttgttagt actttaggag gtcttgcaat agcagcagtt attcctcaaa atcaaataaa
      961 tttaagtgct ggggtagttc aaacatttgt tgttttatta ggtcacttta cagctaataa
     1021 tacaatttta gttaagataa tagctttatt acttgccttt ggtgtaattg cagaagtaag
     1081 ttcatgggtt gtaggaccaa caagaggaat gattatagca gctgaagaag gcgctattcc
     1141 aaaatcatgg gctaaaacaa atgaacatga cgtgccagtt tacttagtta tagcacaagg
     1201 tataatagtt agtatatggg atgctgtttt aacctttggg gcaggtggaa gtaacctttc
     1261 attcttagca gcaatgtctt taacagttgt aatttattta agtggatata ttctattttt
     1321 cgtaggatat ataaaagcta tattaggaga aggattaaat ggtgcttatc aaatgcctgg
     1381 gggaaaacca gttaagatta tagtagccat aattggactt gcaacttcta tatttgcatt
     1441 ctttatatcc tttgtcccac caacatcaat tgcaggaaat gcagttcaat cacatgaata
     1501 tatgttgatg cttattataa gttatgtaat atcattaata ttaccatttg ctatttatga
     1561 tttatgtcaa aaatacatgc attctcatga aggaaatatc catcatatag tagtaggtca
     1621 taaacattaa tttaaagaag gctgtcctaa agagttgata actcatcttg tttaggaga
//
LOCUS       DAEVGI010000269         1678 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEVGI010000269 DAEVGI010000000
VERSION     DAEVGI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.271"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(28..564)
                     /locus_tag="K8M77_002529"
     CDS             complement(28..564)
                     /locus_tag="K8M77_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI7086960.1"
                     /translation="MNVNTNSKFHKTVFTALMIAIIFISANLIRIPTVGGFVHLGDCM
                     VLLAAALLGKKRGALASGIGMALVDLYSGYIIWAPFTFIIKALMAYIAGAILEYNHRK
                     SYLVPFLISGIFMVVAYFFSGAIIAFLFTGSSNTIIGALAYSAKDIIGNILQVGVGIV
                     IALPLSKILYKQENKVFN"
     gene            735..1469
                     /locus_tag="K8M77_002530"
     CDS             735..1469
                     /locus_tag="K8M77_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBI7086961.1"
                     /translation="MKEKNIAAFFDIDGTIYREGLITEVFKKIIKYELVDETKWYKDV
                     RPSFIKWDKRQGGYDEYLLKMVDIYTEAIKGISSYHIEYIAKKVIEQKGDRVYTFTRE
                     RIKWHKEQGHIVIAISGSPYELVKEMSEKYNMNDFKGTIYKLDHNNTYNGDIIPMWDS
                     ESKEKAILELKEKHNIDLENSYAYGDTSGDFTMFKNVGIPYAINPTRELITKVLQCDE
                     IKEKIKVIVERKDVTYDLDINNLKLL"
BASE COUNT          694 a          195 c          225 g          564 t
ORIGIN      
        1 ttgttttttt atatttaaat aaaaactcta attaaagact ttattttctt gtttatataa
       61 tatttttgaa agaggtaagg ctataactat accaacccca acttgaagta tattacctat
      121 tatatccttt gctgaatatg ctaaagctcc tattatggta ttagaacttc ctgtaaataa
      181 aaatgctatt attgcaccag agaagaaata agctacaacc ataaatatac cagagattaa
      241 aaatggtact aaataacttt ttctatgatt atactctaaa atagctccag ctatatatgc
      301 cattaaagct tttattataa atgtaaaagg tgcccaaatt atatatccac tatataaatc
      361 cacaagagcc attcctatac cactagctaa agctccacgc tttttcccta ataaggctgc
      421 tgctaaaaga accatacaat ctccaagatg aacaaagcct cctacagtag gaattcttat
      481 taaatttgct gatataaata ttattgcaat cattagtgct gtaaaaactg tcttatgaaa
      541 tttactgtta gtgttaacat tcatattttt ctacctccag aaccaaaata tatttatata
      601 ttaaaatatt attagtgtat tatcaaatgt atgggtacaa ttataaataa ttaaatccct
      661 ttaaaattta tttatttatg atatactgtt aaataataca taaatagaaa ttttacagaa
      721 gaggagtgaa gaaaatgaaa gaaaaaaaca tagcagcatt ttttgatatt gatggaacaa
      781 tatatagaga aggattaata acagaagtat ttaaaaaaat aataaaatac gaattagttg
      841 atgaaactaa gtggtataag gatgtacgtc catcattcat aaaatgggat aaaagacaag
      901 gtggatatga tgaatattta ttaaaaatgg ttgatatata tactgaagca ataaaaggaa
      961 taagcagtta tcatatagag tatatagcta agaaagtaat agaacaaaaa ggagatagag
     1021 tttatacatt tactagagaa agaattaaat ggcataaaga acaaggtcat attgtcatag
     1081 ctatttctgg atctccttat gagcttgtaa aagaaatgtc agaaaaatac aatatgaatg
     1141 attttaaagg aactatatat aagcttgatc ataataatac atataacgga gatataattc
     1201 caatgtggga ttctgaaagc aaagaaaaag ctatcttaga attaaaagaa aaacataata
     1261 tagacttaga aaatagttat gcttatggag atacctctgg agattttaca atgtttaaaa
     1321 atgttggaat accatatgca attaatccaa caagagagct tatcactaaa gttctccaat
     1381 gtgatgaaat taaagaaaaa attaaagtaa tagttgaaag aaaagatgtt acttatgatt
     1441 tagatataaa taacttaaaa cttctttaga ttataaagta gtatatattt atcaatctta
     1501 taattcgtct ttttataaaa aaatacggag ttatattttt taagcagcaa caaatatacg
     1561 ttattttacg aatttattgc tttaaattta gttttttatc cgttgtatat tactgctaaa
     1621 gtatgtattt aaaattaaaa acttatatta aaatatagaa attttttata gttttaaa
//
LOCUS       DAEVGI010000270         1672 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEVGI010000270 DAEVGI010000000
VERSION     DAEVGI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.272"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(41..1063)
                     /locus_tag="K8M77_002531"
     CDS             complement(41..1063)
                     /locus_tag="K8M77_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBI7086962.1"
                     /translation="MNKDMRDKARELYNGSCRVCKVCNGVACAGEVPGMGGKGTGDSF
                     IRNVAALEKIKLNMRTIHDAKNPTTNIEIFGKNMELPLFAAPITGTMLNMGGKVSERE
                     YIEGVVKGCLDSGIYPMVGDTAVDLCLATNLEVIEEYNGQGIIFIKPWKNEVVIEKIK
                     MAEKAGAFAVGVDIDAAGLITLAMNGKPVEPKNLEEIKELVNSTKLPFILKGIMTPDE
                     AELAVEAGVDAIVVSNHGGRVLDQTPASCEVLKEIAARVKGKVKILVDGGVRTGVDIL
                     KMIALGADCVLIGRPFITATFADGAKGVEEYVNSLKGELKSAMVLTGCNSIENIDNRV
                     IYKREN"
     gene            complement(1077..1520)
                     /locus_tag="K8M77_002532"
     CDS             complement(1077..1520)
                     /locus_tag="K8M77_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBI7086963.1"
                     /translation="MILISGCLCGVNCKYNGENNLNKNILKFLKEGRAVIACPEQLGG
                     LKTPRIPSEIVGGTGKDVLEGKAKVLGKDGTDVTKEFLKGAYETLNIAKSINAEYVIL
                     KAKSPSCGLGVIYSGNFNGEKKEGNGVTCELLLQNGFKVKTENDF"
BASE COUNT          494 a          364 c          146 g          668 t
ORIGIN      
        1 taaaaaatta cttattattt taaataatta ttttacttta ttaattttct cttttgtaaa
       61 taactctgtt atctatgttt tctattgagt tacatcctgt taaaaccata gctgatttta
      121 attcaccttt taaagaattc acatattctt ctaccccttt agctccatca gcaaaggtag
      181 ctgttataaa aggtcttcct attaaaacgc aatcagcacc taaggctatc atttttaata
      241 tatcaactcc agttcttact ccaccatcaa ctaaaatttt aactttacct ttaactctag
      301 ctgctatctc ttttaacact tcacatgagg ctggagtttg atctaaaact cttcctccat
      361 gattagatac tactatggca tctacccctg cttctactgc taattcagcc tcatctggcg
      421 tcattattcc ctttaatata aaaggtaatt ttgttgaatt tactaattct tttatttcct
      481 ctaagttttt aggttcaact ggctttccat tcatagctaa agttataaga ccagctgcat
      541 ctatatctac tcctacagca aaggctcctg ctttttctgc catttttatt ttttctatta
      601 caacttcatt tttccaaggt tttataaata ttattccttg tccattatat tcttctatta
      661 cttctaaatt agtagctaaa cataagtcta ctgctgtatc tcctaccata ggatatatcc
      721 cagaatctaa gcatccctta actactccct caatatattc tctttcacta acttttccac
      781 ccatatttaa catagttcct gttataggag ctgcaaaaag tggtaactcc atattcttac
      841 cgaaaatttc aatatttgta gtagggtttt tagcatcatg aattgttctc atattaagtt
      901 ttattttctc taaggccgct acatttctta taaaagaatc tcctgttcct tttcctccca
      961 tgccaggaac ttctcctgca catgctactc cattacaaac cttacatact ctacaacttc
     1021 cattataaag ttctctagct ttatctctca tatccttatt catctataca ttcctcctaa
     1081 aaatcatttt ctgttttaac tttaaatcca ttttgaagta gaagttcaca agttacccca
     1141 tttccttctt ttttctctcc attaaaattt cctgaatata taactccaag accacaagat
     1201 ggacttttag cttttaaaat tacatattct gcatttatac tctttgctat atttaaagtt
     1261 tcataagccc ctttaagaaa ttcttttgta acatcagttc catccttacc aagcaccttt
     1321 gcttttcctt ctagtacatc ttttcctgtt ccccctacta tttcacttgg tattcttgga
     1381 gtttttagtc ctcctaattg ttctggacag gcaattactg ctcgcccttc cttaagaaac
     1441 tttaatatat ttttatttaa attattttca ccattgtatt tacaattaac tccacacaaa
     1501 cacccactaa ttaaaatcac aaatttctcc tccctaaaac ttattaaaat aaatttactc
     1561 ctagttttat aaaaatttat caattattta ttaacaaaat tctattttat tataatcctt
     1621 aaaacttcat aaaaaaagta tttatctttt aaagtaaaaa agagttatat ca
//
LOCUS       DAEVGI010000271         1655 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEVGI010000271 DAEVGI010000000
VERSION     DAEVGI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.273"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            120..1475
                     /locus_tag="K8M77_002533"
     CDS             120..1475
                     /locus_tag="K8M77_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBI7086964.1"
                     /translation="MSLLKKLTLSLMAVFTTVAFIACSGNKSNETNNAIKSLSTKEVQ
                     ENLKDNSWIAVDTRLSDSYNGWILYDEARGGHIPNAEDFSANWIKVDAKDKENKLDEA
                     LKTKGITTDKNIILYDANGEDSKVVADYLSKKGYKNLYSYDIKEWASDKDLPLEKYED
                     YSLIVPAKILKEVIDGNKPETFENAKNIKVLEASWGDENQTYGKGHVPTSVHINTDTI
                     EPPPTWMLADDKTLTQFASDYGINKDDTIILTGAEQMAAYRLASVLKYMGVKDVRVLN
                     GGDGAWTGAGYKLETKVNPKTPGVDFGATIPADPEVITTIPELKEDLKKSDFTLVDNR
                     TWDEYIGKISGYSYHDKKGRIEGAVFGYAGVNGPTSLEYYRNVDNTMRNGYEIEKMWE
                     DQGINLKNHLAFMCGSGWRAAEVYTYANVMGLKNTSIYSDGWIGWSNGGNPSITGEPT
                     K"
     gene            1479..>1655
                     /locus_tag="K8M77_002534"
     CDS             1479..>1655
                     /locus_tag="K8M77_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086965.1"
                     /translation="MDKIKKILYPIIAIIQTILWIGVIAIQYLTNKKAGVMHHVYFRK
                     YQYSNSISIENLNIL"
BASE COUNT          660 a          177 c          318 g          500 t
ORIGIN      
        1 atttaataaa attagtaaat tggtgtgaat ttaataattt ttaatagtta tttactaaat
       61 aatataaatt tgatattatt tttaatggca ctaaaattaa caaaactaag gaggaaaaga
      121 tgagtttatt aaaaaaatta actttaagtt taatggcagt atttacaact gtagctttta
      181 tagcttgttc tggaaataaa agtaatgaaa caaataatgc tattaaatct ttaagcacaa
      241 aagaagtaca agaaaattta aaagataatt cttggatagc tgtggataca cgtttaagtg
      301 attcatataa tggatggata ttatatgatg aagctagggg aggacacatt ccaaatgcag
      361 aggacttttc tgcaaattgg ataaaggttg atgctaagga taaagaaaat aaattagatg
      421 aagcactaaa aactaaaggc ataactacag ataagaacat aatattatat gatgctaatg
      481 gagaagattc aaaggtagtt gctgattatc tttcaaaaaa aggatataaa aatctttatt
      541 catatgatat taaagagtgg gctagtgata aagatcttcc tttagagaaa tatgaagact
      601 atagtttaat agttcctgct aaaatattaa aggaagttat tgatggaaat aaaccagaaa
      661 cttttgaaaa tgctaaaaac ataaaggtac ttgaggcaag ctggggagat gaaaatcaaa
      721 catatggaaa ggggcatgtt cctacaagtg tacatataaa tacagacact atagaaccac
      781 ctccaacttg gatgcttgca gatgataaga cattaactca atttgcgtca gactatggaa
      841 ttaataaaga tgatacaata atacttacag gtgctgagca aatggcagct tatcgtttag
      901 ctagtgtttt aaaatacatg ggtgttaaag atgtaagagt attaaatggg ggagatggag
      961 cttggactgg tgctggatat aaattagaaa ctaaggttaa tcctaaaact ccaggagtag
     1021 attttggtgc tactatacca gcagatcctg aagtaattac tactatacca gagcttaaag
     1081 aagatcttaa aaaatctgat tttactttag ttgataaccg tacttgggat gaatatatag
     1141 gtaagatatc aggttatagc tatcatgata aaaaaggacg tatagaaggt gctgtttttg
     1201 gatacgcagg agtaaatggt ccaacatctt tagagtatta tagaaatgta gataatacaa
     1261 tgagaaatgg atatgaaata gaaaaaatgt gggaagatca aggaataaac ttaaaaaatc
     1321 atcttgcatt tatgtgtggt agtggatggc gtgcagctga ggtttacact tatgctaatg
     1381 taatgggact aaaaaataca tctatataca gtgatggatg gattggttgg agtaatggtg
     1441 gaaatccatc aattactgga gagcctacaa aatagttcat ggataaaata aagaaaatac
     1501 tatatccaat aatagcaata attcaaacca tattatggat tggggtaatt gctattcaat
     1561 atttaactaa taaaaaagct ggtgtaatgc accatgttta ttttaggaaa tatcaatatt
     1621 caaatagcat aagtattgaa aatttaaata tttta
//
LOCUS       DAEVGI010000272         1644 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEVGI010000272 DAEVGI010000000
VERSION     DAEVGI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.274"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            110..1557
                     /locus_tag="K8M77_002535"
                     /pseudo
     CDS             110..1557
                     /locus_tag="K8M77_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
BASE COUNT          642 a          199 c          173 g          630 t
ORIGIN      
        1 taagttttat caaaaggtat tttgataaat gccttttttt aatttcaaac ataaatttat
       61 atattaagtg gaataataga tttattatat attaatttaa gtgatgttta tgattgataa
      121 agttataaag aaatacaatt tagatgtgga ttcaatgaaa cgggaaggac aattgcctgt
      181 ttaacatttt tagcctcttg gatatccttt ggaattaata atgcaatctt agcatatcca
      241 atagctttaa cctcctcaat tcttttaaaa gaaaatttta aaataaatcc cttagaaaaa
      301 actataagac ttttattttt atattgtttt atagttgttt tatctttttt atcatcaaac
      361 aattttttat taggaattat aataaacttc ttcaccatat ctttcatagc ttataagctc
      421 tcagttacct acgctccttt attatacaaa ccattcttaa tgttatatgt atttacttat
      481 ttttataagg tagactttca aggcttacct agaagattat tatctatttt ctttggcttt
      541 agtttaataa taatctttca cttattttta aacaagctaa gttataaatc cttaataaaa
      601 gatagtataa ataaatcttt atttttatta gaaagccaag tatataacct tatttttaaa
      661 agttataatt cagatttgca gcaatctatc tctaaagaac ttactactat ttgttataac
      721 ctttatacta caagaaagag aaaaatttta actaataact taggtagtat tcaatttaaa
      781 atatatataa tattagaaaa cttaaactta gatttatata atctaaataa gctctactca
      841 aagcttaatt acaatagtgc cctaattaaa tcatttttaa agaaattaaa aaaatccata
      901 aatattttaa gattatattt aaatgatgag atttcttatt atgaaataga taaacaacta
      961 aataaattaa ataggtttca tgaagattta cctgatcagt tcactttttt tcatgactta
     1021 tctatttcag taactaaagc tatttttaga gttttttatt tagctttagg tatattaata
     1081 accttcttag ctaataaatt tttatttcct tataacttag atcaagggct aaaagaatta
     1141 agtcttaaat taataaaata tgtaaatata ttagcagaaa atctaataaa aaacccttca
     1201 aaaaatgaag aggaaataat aaatttaacc atccatataa agcttatgtg taacaagctt
     1261 gcacttagaa atattcaaaa aaaagataag gatataaata gattaatact cttaactgga
     1321 aatcttactg cttctttaag ctactatact ctcttaaaga aagatttagg attggtatgt
     1381 gggataaata aagatgagtt aattaagctt caaagtaaat tacaatattc ccttaaagaa
     1441 aaggtgccat taatagatat aataaaccta ttagactcaa cagttgattc ccttattaat
     1501 tgtccttact ctagaaaagt aatttataac cctgcctcta atgggtttat atattaatta
     1561 aagcttattt cttaagtaac tatctcatta taagcaaact tagaatgaaa attataatga
     1621 gataaaaaac ttattgagat tatt
//
LOCUS       DAEVGI010000273         1621 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEVGI010000273 DAEVGI010000000
VERSION     DAEVGI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.275"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            84..1067
                     /locus_tag="K8M77_002536"
     CDS             84..1067
                     /locus_tag="K8M77_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086966.1"
                     /translation="MENRNEDNLIDEFNELVSEISKSVFDKSIYRDLKLTEENLIKTL
                     TSTSEKTATINRNNLISLQNNLKVILDEAIADFNLGVKQGEEAFEKKANEFLNNIEEI
                     STKSKENIYHLKDKAKETYDEIERLKEQVEGINKNIGELSKEEGIENIATLQRIGREL
                     SEKIRTSNHIAKEHIEKIEEMDKMLKGECLRVMNCVNKLENRLKVDKSEIKDNFLGFK
                     EYMGEGFEEINYNIKLEFNEIKDLLEEGNTNMNNSMELLNSAIDKYNVDVIDATRRLG
                     ERVNNLDRKISNMNKGSDESFFHEELKSELDKKYYFLVISIVIVGILSVLF"
     gene            1077..>1621
                     /locus_tag="K8M77_002537"
     CDS             1077..>1621
                     /locus_tag="K8M77_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086967.1"
                     /translation="MLVKEYEVKVSKDKEKNKVLYNSPSLEEKFLKLYNHIKEYEKEN
                     KLLIMVLKLKEYINDLKIELEYLEKYAGSNRENNKINSFLRKSKKEFMDIDIKLNSIT
                     KDNIKSLQEDLIDEVLLNLKYKLLESIKEYSEEINKDYLFHNKLRVDFGIQCKELKEK
                     FLEELKIYLESSSKIIKKNIN"
BASE COUNT          737 a          110 c          295 g          479 t
ORIGIN      
        1 atgactttta atgaagagat ttagtaataa atgaagtttt atggtatata aactcatatt
       61 aggtagtggg gtaggaggaa gttatggaga ataggaatga agataactta atagatgaat
      121 ttaatgaatt agtttcagaa ataagtaaat cagtctttga taaatctatt tatagggatt
      181 taaaacttac agaagaaaat ttaataaaga cccttacaag tacttcagaa aaaactgcta
      241 caataaatag aaataactta ataagtttac aaaataattt aaaggtaata ttagatgaag
      301 ctatagcaga ctttaattta ggggttaagc aaggggaaga agcctttgag aaaaaagcta
      361 atgagttttt aaacaatata gaagagatat ctactaagtc aaaagaaaac atttaccatt
      421 taaaagataa ggcaaaggaa acctatgatg aaatagaaag gttaaaggag caagtagagg
      481 gaataaataa aaatataggg gaactatcaa aagaagaagg aattgaaaat atagctactt
      541 tacaaagaat aggcagggaa ttaagtgaaa agataaggac aagcaatcac attgctaagg
      601 agcatataga aaaaatagaa gagatggata aaatgctaaa aggtgaatgc ttaagagtta
      661 tgaactgcgt caataaacta gaaaataggt taaaggttga taaaagtgaa attaaagata
      721 atttcttagg ctttaaggaa tatatggggg aaggttttga agagataaat tacaatatta
      781 agttagagtt taatgaaata aaggatcttt tagaagaggg aaatacaaat atgaataact
      841 ctatggaatt attaaatagt gcaattgata agtataatgt tgatgtaata gatgccacaa
      901 gaaggcttgg agagagagtt aataatttag atagaaaaat atctaatatg aacaaggggt
      961 ctgatgaatc atttttccat gaagagttaa aaagtgaatt agataaaaaa tattactttt
     1021 tagtaattag catagtaatt gtaggcattt tatcagtttt attttaggag aaagccatgt
     1081 tagttaaaga atatgaagta aaagtttcta aagataaaga gaaaaataag gttctttata
     1141 attcaccgag tttagaagag aagtttttaa aactatataa tcatataaaa gaatatgaaa
     1201 aggaaaataa actattaata atggttttaa agttaaaaga atatataaat gatttgaaaa
     1261 tagaattaga atacttagaa aaatatgcag gtagcaatag ggagaataat aaaataaatt
     1321 cttttttgag gaaatcaaaa aaagaattta tggacatcga tataaagtta aactccataa
     1381 ctaaagataa tattaaatct ttacaagaag atttaataga tgaggtttta ttaaatttaa
     1441 agtataaatt attagaatct attaaagagt atagtgaaga aataaataag gattatttat
     1501 ttcacaacaa gttaagggtt gattttggaa ttcaatgtaa ggagttaaaa gaaaagttct
     1561 tagaagaatt aaaaatttat ttagaaagtt cttctaaaat aattaaaaaa aatattaatt
     1621 t
//
LOCUS       DAEVGI010000274         1580 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEVGI010000274 DAEVGI010000000
VERSION     DAEVGI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.276"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            29..1537
                     /locus_tag="K8M77_002538"
     CDS             29..1537
                     /locus_tag="K8M77_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBI7086968.1"
                     /translation="MSKKKKISFPTAFTVLFIVLILSAILTYVIPAGSYSKLSYNEAE
                     NTFVVTNPQGESTKENATQNTLDKLGIKINLSKFTDGSINKPIAIPNTYEKVSQNPQG
                     ISKIIEAPIQGTYDTIDIIMFVLIIGGVIGVLNATGAFNAGIASLSKITKGKEYILII
                     LLSILISLGGTTFGLAEETIALYPILLPIFLASGYDAIVCIATIYMGSSIGTMFSTVN
                     PFSSVIASTAAGISFKEGLYFRMIGLVLATLITIIYILRYAKKVKNDPSKSLVYDQKD
                     EIDSKFLHESNNDVPVFTWRLKLMLLIFAGSFVILVYGVSAKGWGFIQMTALFLVVGI
                     ILGFLSGLGEKKFVNTFIAGAADLVGVALVIGVARSINLILENGKISDTLLYVSSNGI
                     QGMDKNIFIILMLVIFIILGFFIPSSSGLAVLSIPIMAPLADTVGLPRDVIVSAYQFG
                     QGLISFITPTGLILATLAMVDVTYNKWLKFIMPLMGIIAAFAALLLLVQVHF"
BASE COUNT          543 a          226 c          241 g          570 t
ORIGIN      
        1 aattataaaa tttaatagga ggtaaaaaat gagtaaaaag aaaaaaattt cattccctac
       61 agcctttact gtattattta ttgttttaat tttatcagct atattaactt atgttattcc
      121 agcaggatca tattcaaaat tgtcttataa tgaagctgaa aacacctttg ttgttacaaa
      181 tcctcaaggg gaaagcacta aggaaaacgc aactcaaaat accttagata aacttggtat
      241 aaaaataaac ttaagtaaat ttactgatgg aagtataaat aaaccaatag ctataccaaa
      301 tacttatgaa aaggtttctc aaaatcctca aggaatttct aaaataatag aagctcccat
      361 tcaaggaact tatgacacta tagatataat tatgttcgtt ctaataatag gtggagtaat
      421 tggagtttta aatgctactg gagcatttaa tgccggaatt gctagccttt ctaaaataac
      481 taaaggaaaa gaatatatac ttataatatt attatcaata cttatttctc ttggtggtac
      541 tacttttgga ttggcagaag aaacaattgc tctttatcct attttactcc caatattcct
      601 agcttctggc tatgatgcta tagtatgtat tgctacaata tatatgggtt catctatagg
      661 aacaatgttc tcaactgtaa acccattctc ttcagtaata gcttcaacag ccgctggaat
      721 aagctttaaa gaaggccttt attttaggat gataggatta gttttagcta cacttataac
      781 aataatttat atacttagat atgctaaaaa agttaagaat gatccttcca aatcccttgt
      841 atatgatcaa aaagatgaaa tagattctaa atttcttcat gaatctaata atgatgtgcc
      901 agtatttact tggagactta aacttatgct tttaatattc gctggttcat ttgtaatttt
      961 agtttatgga gtttcagcta aaggatgggg atttatacaa atgactgctc tattccttgt
     1021 agttggaata attttaggtt tcctttcagg acttggagaa aagaaatttg ttaatacatt
     1081 tatagctggt gctgctgatt tggtaggagt tgccttagtt ataggtgttg caagatctat
     1141 aaacttaata cttgaaaatg gtaaaatatc agatacttta ctttatgtat cctcaaatgg
     1201 aattcaaggt atggataaaa atatatttat aatattaatg cttgttatat tcataatctt
     1261 aggattcttt attccatctt catctggtct tgctgtttta tcaattccaa taatggcacc
     1321 acttgcagat acagttggtt taccaaggga tgttatagtt agtgcttacc aatttggtca
     1381 aggattaatc tcctttataa ctccaacagg attaatttta gctacccttg ctatggttga
     1441 tgtaacctat aataaatggc tgaaatttat tatgccttta atgggaatta tagcagcctt
     1501 tgcagcctta ctattattag tacaagtaca cttttaaaac atttaaaata ttagtgtatt
     1561 aaaatcactt ttttataaaa
//
LOCUS       DAEVGI010000275         1554 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEVGI010000275 DAEVGI010000000
VERSION     DAEVGI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.277"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            19..729
                     /locus_tag="K8M77_002539"
     CDS             19..729
                     /locus_tag="K8M77_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBI7086969.1"
                     /translation="MEKTCTCGSCKELCAKNISIFSTLSNEELLKIVNMTEHKSFKEN
                     DVLCREGEKSDKLFLIREGRVKICKITKEGKEQIVYVLSKGDFFGENNLFTSNNISNF
                     SAYAITDAKLCILKKENLEKILIKNPEISLKIIQEMADRINSAENLAQTLATRDVEAR
                     LATVLIEFMDKYGEKNKEGIYISLPLNREQIANYCGITRETVSRRLSKFDKLGIIKLQ
                     GNKGLIVKDKEALFSLAE"
     gene            complement(982..1143)
                     /locus_tag="K8M77_002540"
     CDS             complement(982..1143)
                     /locus_tag="K8M77_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HBI7086970.1"
                     /translation="MEKFVCDVCGYIYDPVVGDPDNGVASGTEFKDIPDTWVCPLCKL
                     DKTHFSKVE"
BASE COUNT          636 a          144 c          234 g          540 t
ORIGIN      
        1 gtaaggagga agatagaaat ggaaaaaaca tgtacatgtg gatcatgtaa agaactttgt
       61 gctaaaaata tatctatttt ttcaacatta tctaatgaag agttgttaaa aatagttaat
      121 atgactgaac ataaaagttt taaagaaaat gatgttttat gtagagaagg ggaaaaatca
      181 gataagcttt tcttaattag agaaggcagg gtaaagatat gcaaaataac taaagaggga
      241 aaagaacaga tagtttacgt attatctaaa ggtgatttct ttggagaaaa taatcttttt
      301 actagtaata atattagcaa cttttcagct tatgcaataa cagatgcaaa gttatgtatt
      361 ttaaaaaagg agaatttaga aaaaatactc ataaaaaatc cagagatatc attaaaaata
      421 atacaagaga tggcagatag aataaattct gctgagaatt tggcacaaac tttagctact
      481 agggatgtag aagcaagatt agccactgta ttaatagaat ttatggataa gtatggtgag
      541 aaaaataaag aaggaatata cataagttta cctctaaata gagaacagat agcaaattat
      601 tgtggtataa caagagaaac tgtaagtaga agactttcaa aatttgataa gttaggaata
      661 ataaagttac aggggaataa gggtcttatt gttaaggaca aagaagcttt atttagttta
      721 gctgaatagt taaaaggaga ttatatatta gtaagttgaa attttttaac aaggctatat
      781 ttttaagtcg gatttgattt tagactcact taatgattta gttattaata agtggtttat
      841 agcttcaatc aaatttactt taaaatatag cctttaaata aataataatc tttataaaac
      901 ttttgatttt tataatagaa ctaattagat atactgtttt agtctaagaa tgtattctta
      961 agtcttatat agacttctaa attattcaac tttagaaaaa tgagttttat ctagtttaca
     1021 aagtggacat acccaagtat ctggaatatc tttaaattcg gtaccagaag ccacaccatt
     1081 atcaggatct cctacaactg gatcataaat atatccgcaa acatcacata caaacttttc
     1141 cattattaaa acctccttag aattaataga tatgttattt tattatgttt aattattaaa
     1201 taaattatgt ctaaaaatat taattagtat tagatttatt agaagttata atatatttaa
     1261 tttaaaaaat cccaatgagt atagaagaat tagaattttt gtatttggat agtatattat
     1321 taaagtcaaa gatttgtttt gggtaaatat aatttttaaa tgtgatgcca gtcatatttt
     1381 agagcaatta ttgtagatat aataacttta taatagagaa gtaaagacta agatgttaat
     1441 attaatagca tataaacaca tattaatagc tttatatata taatatttaa ataaacttat
     1501 aattttcgtt tttattttta taaaacctat aaattaagaa tttcggtgta caaa
//
LOCUS       DAEVGI010000276         1519 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEVGI010000276 DAEVGI010000000
VERSION     DAEVGI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.278"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(42..1463)
                     /locus_tag="K8M77_002541"
     CDS             complement(42..1463)
                     /locus_tag="K8M77_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HBI7086971.1"
                     /translation="MDLTSNFDKNINTLSKALRVGKSFDIIERSIIIGDKKATMYYID
                     GFVKDDVMELIMSDFFSLSKTEMNLIKSPKDFMRRQIPYVEVAEEVSIDKIVSQVLCG
                     QTALILEGFSSAIMIDLRTYPVRGPQEPETEKVLRGSRDGFVETIVFNTALIRRRIRD
                     PRLTFEMMSIGNVSKTDVVISFLDEVVDKKTLNLIKKKFENLDIQALTMSEQSLVESL
                     SNTSWYNPFPKVRYTERPDVAAAHITEGKIVIIIDNSPSVIILPTTIFDFIQDVDDYY
                     LPVITGNFIRLIRNFILISTIFITPLYLLLIQNAYRIPDYLKFLLPVDGYKVPLIVQF
                     LLLEVAVDGLKLASLNTPNALGMSLSVIGGLILGQFAVDTGLFLPQTILYMAIVTLGS
                     FSQPSLELSYALKFWRTLLLILTSLFNVIGFFVGLIIGIVLIASNKTLTGDSYLYPLI
                     PLDWKALKALIFRVRLVPKQKEE"
BASE COUNT          535 a          234 c          211 g          539 t
ORIGIN      
        1 tatttattaa atgaaaaatt aagaaaatca acaaattttt attattcttc tttttgttta
       61 ggtactaatc ttactctaaa gataagtgct ttaagtgctt tccagtcaag gggaataaga
      121 ggataaagat aggaatcacc agttaaagtt ttattgcttg caattaaaac tattcctatt
      181 attaatccta caaaaaatcc tattacatta aataggcttg ttaatatgag taggagtgtt
      241 ctccaaaact ttaaggcata gctaagttct aaactaggtt gagaaaagct tcctagtgta
      301 actatagcca tatataatat ggtttgtggt aaaaacaacc cagtatctac agcaaattgg
      361 ccaagtatta atcctccaat aacagataag gacataccaa gggcatttgg cgtatttaaa
      421 gaggcaagtt ttaatccatc tactgcaact tcaagaagta aaaattgaac tatcaatgga
      481 actttatatc catcaacagg taataaaaat tttaagtaat caggtattct ataggcattt
      541 tggattaaaa gcaaataaag tggggttata aaaatagtag aaattagtat gaaatttctt
      601 attaaacgaa taaaattgcc tgttattaca ggaagataat aatcatcaac atcctgtata
      661 aaatcaaaga tagtagttgg taaaattata actgaaggag agttatctat aataataact
      721 atctttccct ctgttatatg ggcagctgca acatcaggcc tttctgtata tctaacctta
      781 gggaatggat tataccatga ggtatttgaa agagattcaa caaggctttg ttcgctcatg
      841 gttaatgctt gtatatctaa attttcaaat ttctttttta ttaaattaag agttttttta
      901 tcaactacct cgtctaaaaa tgatataacc acatctgttt ttgatacatt tcctatagac
      961 atcatttcaa aggtaagtct aggatctctt attcttcttc taattaatgc agtattaaat
     1021 acaatggtct ctacaaagcc atccctagaa ccccttaata ctttttcagt ttctggttct
     1081 tgaggtcctc taactggata agttcttaaa tctatcatta ttgcactgga gaatccttct
     1141 aaaattaatg ctgtctgtcc acagagaact tgagatacta ttttatctat ggatacttct
     1201 tcagctactt ctacataagg gatttgtctt ctcataaaat ccttaggtga ttttattaag
     1261 ttcatttcag ttttgcttaa ggaaaagaag tcactcatga ttaattccat tacgtcatct
     1321 tttacaaatc catcaatata atacattgtg gcttttttat ccccaattat tattgaacgt
     1381 tcaattatat caaaactttt tccaaccctt aaagctttag ataaagtatt tatattttta
     1441 tcaaaatttg atgttaagtc caaaaaaaat tcctccttag taaataatat aaacttatta
     1501 tttcaaagga gaataaata
//
LOCUS       DAEVGI010000277         1505 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEVGI010000277 DAEVGI010000000
VERSION     DAEVGI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.279"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..486
                     /locus_tag="K8M77_002542"
     CDS             <1..486
                     /locus_tag="K8M77_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7086972.1"
                     /translation="ELILFLYRYYLCSFTEDIQKALEDALELNSMFRQALSEREVIRA
                     TRSAERCYLDKNKQYKYKNETLIELLEITEEEQKYMTIIISKKEYKRRENIRGKKNYQ
                     EKLKSQGKFTEKEKISQRRKKILDLLAEGLKQKDICIFLNISKPTYVRDRNFLKEQGL
                     I"
     gene            1020..>1505
                     /locus_tag="K8M77_002543"
     CDS             1020..>1505
                     /locus_tag="K8M77_002543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086973.1"
                     /translation="MLRGVDEVAKELKVSKTAIYNKLKLKEFKHKVVKKQGKSMIDEE
                     LLTLLKDSLKVKNKVEYKDYIEENKGEVKSEIAIDREGSLNLNKSLIDALIVQLEEKD
                     KQIAELHKLIENNQVLLKKEQETKVNILELEDHFKEVDNKLNSIKEKMNQRKEKKSFF
                     KN"
BASE COUNT          633 a          127 c          255 g          490 t
ORIGIN      
        1 gaacttatat tatttttata tagatattat ctttgtagct ttacagaaga tattcaaaag
       61 gctttagaag atgctttaga actaaatagt atgttcagac aagctttaag tgaaagagaa
      121 gttataagag caactagaag tgctgaaaga tgttatttag ataaaaataa gcaatataag
      181 tataagaatg aaactcttat agagctatta gaaattacag aagaagaaca aaaatatatg
      241 acaataataa tttctaaaaa ggaatataag agaagagaaa atattagagg taaaaaaaat
      301 taccaagaga aattaaaatc tcaaggtaaa tttactgaaa aagaaaaaat ttcacaacgt
      361 agaaaaaaaa tattagacct tttggcagaa ggtctaaaac aaaaagatat atgtatcttt
      421 ttaaatatat ctaaaccaac ttacgttcgt gatagaaact ttttaaaaga acaaggttta
      481 atataaccat gttctttttg tattataaaa taaaatttta tattaagcaa ttaactttta
      541 aatagtttta aataggtatc aaatttttta cgcttgtatt atagggtact ttgtacctgt
      601 tctttttttg gggaggttgt aaagataatt ttttacttta gttagttccg aaggaacttt
      661 tattatagtg cccctttaat tttttgcgtg gcattgaatg taaaaaatta tcactatact
      721 agggcgtaaa gtaatattat atgtgtctca aagtgggatt aaagcgggat tttatagggc
      781 gtttttgtgg cttagagtgg gataattgga aattactttg atagcattga aatactaggc
      841 tttgactaga ctagaacctt tggtgatagc aatttctcct tatgctcttt ttaatacttt
      901 caaaaagttt ttccatgaag ttttattaaa gtaattaaat ctataaaaaa ggaaaatcta
      961 ttattgttta gaattaatta aataggatta aagtttaatg gaattagggg aagttagtta
     1021 tgttaagagg tgtagatgag gtagctaagg aattaaaagt tagtaaaact gctatatata
     1081 ataaacttaa gttaaaagag tttaaacata aagtagttaa aaagcaaggt aaatcaatga
     1141 ttgatgagga attattgacc ttgcttaaag atagtttaaa agttaaaaat aaagttgaat
     1201 ataaagatta tatagaggaa aataaaggag aggtaaaatc agaaatagca attgatagag
     1261 aaggttcatt aaacttgaat aaaagtttaa ttgatgcttt aattgttcaa cttgaagaaa
     1321 aagataagca gatagcagag ttacataagc ttatagagaa taaccaggta cttttaaaaa
     1381 aagaacaaga aactaaggtt aatatattgg aacttgaaga tcactttaaa gaagttgata
     1441 ataaattgaa ttcaataaaa gaaaaaatga atcaaagaaa agagaaaaaa agttttttta
     1501 aaaac
//
LOCUS       DAEVGI010000278         1492 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEVGI010000278 DAEVGI010000000
VERSION     DAEVGI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.280"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..226
                     /locus_tag="K8M77_002544"
     CDS             <1..226
                     /locus_tag="K8M77_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7086974.1"
                     /translation="QSVLKIDTNATVINVLTELDARYDPSPNSNRMGYVKNSERLFVH
                     KVENNYALATYLAGEGYKTAWFTSKYIKLD"
     gene            complement(308..880)
                     /locus_tag="K8M77_002545"
     CDS             complement(308..880)
                     /locus_tag="K8M77_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI7086975.1"
                     /translation="MLVGYARVSTEDQNLDRQIDLLIDYGVDKRNIYQEKISGTKTHR
                     EQLDKMIDELQEGDTVIIADLTRISRSTKDLLDIIDRIKAKGASIKSIKDTWLDTSSD
                     NPYNSFLLTVMSGLSQLERDLISQRTKEGLKSAKARGRNGGRPSKRNDKSDTVGLLYK
                     EGYKIVDIVKQTGLSRATVYRVLKDLGLKE"
     gene            complement(1263..>1492)
                     /locus_tag="K8M77_002546"
     CDS             complement(1263..>1492)
                     /locus_tag="K8M77_002546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086976.1"
                     /translation="LNLPDSKTFYFSNEKSNDNYDLKIFKCFSSDKDIKSLKDIIRAE
                     IESVNNEYSICKIIIDVRFGSLTFYFKNKKI"
BASE COUNT          501 a          244 c          123 g          624 t
ORIGIN      
        1 gcaatcagta ttaaaaattg atactaatgc tactgtaatt aatgttttaa ctgaattaga
       61 tgctagatat gatccttcac ctaattcaaa tagaatgggc tatgtaaaaa atagtgaaag
      121 actttttgtc cataaagttg aaaataatta tgcattagct acttatttag caggagaagg
      181 atataaaact gcatggttta catctaaata tataaaactt gattaataaa agtacatcaa
      241 agagaaataa agaagactat aaaaaagtaa taaaaacttt tttatagtct tttttccata
      301 taaactatta ttcttttaat cctaaatcct ttagaactct atatacagtt gctctactta
      361 atcccgtttg ttttactata tccactatct tataaccttc tttataaagc aaacctactg
      421 tatcagattt atcatttctt ttagaaggcc ttcctccatt ccttccccta gcttttgcgc
      481 tttttaagcc ttctttagtc ctttgactta ttaagtccct ttctaattga cttaatccac
      541 tcataacagt taataaaaag ctattatatg gattatcact tgaagtatct aaccaggtat
      601 cttttatact ctttatacta gctcctttag cctttattct atctataata tccaataaat
      661 cttttgtact tcttgatatt ctagttaaat cagctattat aaccgtatct ccttcttgca
      721 gctcgtctat catcttatct agctgctctc tatgagtctt agtaccactt atcttctctt
      781 gatatatatt tctcttatca acaccataat caattaacaa atctatttgt ctatctaggt
      841 tctggtcctc tgtactaact cttgcatatc ctactaacat ctttttttca tttatcaatt
      901 ctatatcaag aactattaaa tttttatcct ttacggattc tatatatctc tccatatcac
      961 ttttgctatt aaatattttt gtataacttc tttttctatt gccatatcta taagttaatt
     1021 tatacatatc attaatctcc attttctata atatctatta attatattgt atcataaagg
     1081 tatcataata aaaatagatt atgatacctt ttttgataca tttttaaact tattaaatgg
     1141 cttgtttact tattaaaaaa aacgtatcaa aaacactcgt ttttgatact gattttataa
     1201 attatttata ataaacttat tatttaaact acttatagtt atataaaatt tactaataaa
     1261 ttttatattt ttttattctt aaagtaaaaa gttaatgatc caaatcttac atctattatt
     1321 attttacaaa tactatattc attattaaca ctttctattt cagctcttat tatatccttt
     1381 aaacttttaa tatctttatc agaactaaaa catttaaata ttttaagatc ataattatca
     1441 ttactttttt catttgaaaa ataaaaagtc ttactatctg gtagatttaa tc
//
LOCUS       DAEVGI010000279         1486 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEVGI010000279 DAEVGI010000000
VERSION     DAEVGI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.281"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>1486
                     /locus_tag="K8M77_002547"
     CDS             <1..>1486
                     /locus_tag="K8M77_002547"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086977.1"
                     /translation="KIDFLEREKSKLIRQEEDILDELDKLEGGRVLELPKEIKKKLEY
                     KDIKIIYGMEWLKKNGYSTEKNIEVVKNNPFIPYSLIMNSSEIEILKREGLDIFISNP
                     ISIINRLDLESNFLEVDQSMVSFNKLNFFVSFNNKLLDEKELIKLIEEKKQYLESIRS
                     NIENRKNEIRFLEKKKEIIQYSKLDKEKYENVKREKTFLEKKIIEIRENEISIAREFG
                     KIEINISELENTKKVLEENIRKNLEKINSFNELIEEYNKYKDNKDELSLIEFKIEEIN
                     KFLKENENRELEIDKTLKDINNIMVSYNNSLEKLKSEAIEFSFYKEGLFIKKDKEDLL
                     AEFYVLNKQITASEKDLKKRREEKSRAFKEIEDELNAKANKYNVAEAEYINEVYNINR
                     DFELKDLIEKEDKNYNYLKDKRSNILQEIASEKSNVENLYESLEKDLGKTKAKSKALL
                     YDKNFKEEIAKLKIEKASNEKKIKEVVNEKNKIDKYLIDLSQFNE"
BASE COUNT          730 a           78 c          246 g          432 t
ORIGIN      
        1 aaaaatagat tttttagaaa gagaaaaaag taagttaata agacaagaag aagatatatt
       61 agatgagtta gataagctag aagggggtag agttttagaa cttccaaagg aaataaagaa
      121 aaagcttgaa tataaagata ttaaaattat ttatggtatg gaatggctta aaaagaatgg
      181 atattctact gaaaaaaata ttgaagttgt aaaaaacaat ccttttatac catattcatt
      241 aataatgaat tctagtgaaa ttgaaatatt aaaaagggaa ggactagata tatttatatc
      301 aaatccaatt tctataataa ataggttaga tttagaaagt aattttcttg aagtagatca
      361 aagtatggtg tcttttaata agttgaattt ttttgtttcc tttaataata agcttttaga
      421 tgaaaaagaa ttgataaagt taatagaaga aaaaaaacaa tacctagaaa gtattagaag
      481 taatatagag aatagaaaaa atgaaattag atttttggaa aaaaagaaag agataattca
      541 atattctaag ctagacaagg aaaaatatga aaatgtaaaa agagaaaaga catttttaga
      601 aaaaaagatt atagaaataa gagagaatga aatatctata gctagagaat ttgggaaaat
      661 agagattaat atttcagagt tagaaaatac taaaaaagtt ttggaagaaa atataagaaa
      721 aaatcttgaa aaaattaata gctttaatga attgatagaa gaatataata aatataagga
      781 taataaagat gaactgagtt taattgaatt taaaatagaa gaaattaata agtttttaaa
      841 agaaaatgag aatagagaat tagaaattga taagacttta aaagatataa ataatattat
      901 ggtaagttac aataatagct tagagaaatt aaaaagtgaa gctatagaat ttagctttta
      961 taaagaagga ttatttataa aaaaagataa ggaagattta ttagcagagt tttatgtttt
     1021 gaacaagcag ataacagctt cagaaaaaga tttaaagaaa agaagagaag aaaaaagtag
     1081 agcatttaaa gaaattgaag atgaattaaa tgctaaagct aataagtata atgtagcaga
     1141 agctgaatat attaatgaag tttacaacat aaatagagat tttgagttga aagatttaat
     1201 agaaaaggaa gataaaaatt ataattattt aaaagacaaa agatctaata tacttcaaga
     1261 gattgctagt gaaaaaagta atgttgaaaa tttatatgaa tctttagaaa aagatcttgg
     1321 aaaaactaag gctaaaagta aggctttgct ttatgataaa aactttaaag aggaaatagc
     1381 aaaattaaaa attgaaaaag cttctaatga aaagaaaatt aaggaagtag ttaatgaaaa
     1441 aaataaaatt gacaaatatt taattgattt aagtcaattc aatgaa
//
LOCUS       DAEVGI010000280         1453 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEVGI010000280 DAEVGI010000000
VERSION     DAEVGI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.282"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            247..804
                     /locus_tag="K8M77_002548"
     CDS             247..804
                     /locus_tag="K8M77_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086978.1"
                     /translation="MKKSVKSLSLAVLIIILSLNIIACNSSNRALDKGKELISEGQYE
                     KAEVSLELALDEDPKNKEANELKNMIENYLEASKALDEGEIRKAEVKIKNIGEKSDEF
                     PNFKKCVDALNKNIDEKSEYDKDIKNDMEKIENFIDNKDYSDAILLTKSLDGRVRTTE
                     QKEKLEQIRLKLIAVLSIENTKINR"
     gene            complement(954..1121)
                     /locus_tag="K8M77_002549"
     CDS             complement(954..1121)
                     /locus_tag="K8M77_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086979.1"
                     /translation="MKQNNLKRNKKIKRKEICEICGEDMNENPFAYDYDSEIKLIFKC
                     PSCGHRKVRNL"
BASE COUNT          562 a          121 c          196 g          574 t
ORIGIN      
        1 cctttctttt ttataatttt ccataaaatt tgagttttat agcaaaaaag cgtactaatt
       61 attcattatt atttttatta gttaagaata tttacttgtt gttcaaaata tgatattatt
      121 tgtttgggat aaaaaggaaa tttaaaaata tggaaatata gggtttttta tggcattata
      181 attttcatta aattatttta ttaagctaaa ttacaataat atagattaga caatgggggt
      241 aattaagtga aaaaaagtgt gaaatcttta agtttagccg tattaattat aattttatca
      301 ttaaatatta ttgcatgtaa tagttcaaat agagcattag ataagggaaa agaacttatt
      361 agcgaagggc aatatgagaa ggctgaagta tctttagaat tggctttaga tgaagatcct
      421 aaaaataaag aagctaacga attaaaaaat atgattgaaa attatttaga agcaagtaag
      481 gctttagatg agggggaaat aagaaaagct gaagttaaaa ttaaaaatat tggagagaaa
      541 tctgatgagt ttcctaattt taagaaatgt gtagatgcgt taaataaaaa tatagatgaa
      601 aaatctgaat atgataaaga tataaaaaat gatatggaaa aaatagaaaa ttttatagat
      661 aataaagatt attcagatgc aatattacta acaaaaagtt tagatggaag agtaagaacg
      721 actgaacaaa aggaaaagct tgaacaaata agattaaaat taattgcagt attatctata
      781 gaaaatacca aaattaatag ataatatagg attatttaga caatatagga taatttatga
      841 agagaatgta gaataattta tgaagagaat gtagaataat ttgtgtaatt taatatttta
      901 tatcgtttat ataactggaa attttatttc cagttatatt tttatgtgaa ctcttataaa
      961 tttctaactt ttctatggcc gcatgatgga catttaaata taagtttaat ttctgaatca
     1021 tagtcatatg caaaaggatt ttcattcata tcttctccac agatttcgca aatttctttt
     1081 cttttaattt ttttatttct ttttaaatta ttctgtttca attttatttt tgctactcaa
     1141 aatatggtca aatatataaa gattattatt tcttaattaa acctagtatt atctaaacta
     1201 ttaagatttt aaatgattct ctcatataat aagcttatat attagataat ttcttatgaa
     1261 ttttttgtgt ttcgactact tggtttgaat attttgacta gtagctctcc tttcttttat
     1321 ttttatattt tcatatttcc ttgaaagttt tatttattca ctttttaaaa tcttttctac
     1381 tttttaatag tatatgtttt atttaaaaaa ataaaaaact tatgttattt tttacttcat
     1441 ggtaaagttt tac
//
LOCUS       DAEVGI010000281         1448 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEVGI010000281 DAEVGI010000000
VERSION     DAEVGI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.283"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            260..1166
                     /locus_tag="K8M77_002550"
                     /pseudo
     CDS             260..1166
                     /locus_tag="K8M77_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
BASE COUNT          542 a          136 c          231 g          539 t
ORIGIN      
        1 tattgaataa ttgttaagat tttctttcaa aaaaagatgt aaaactgaca acttttaaga
       61 aggtaaaaag tttttgattg caataaaaaa ctacattata agctaatttg agcaatttta
      121 agatataaca actaaaatta tatgataggg cagaaaatga aagttaataa aagtaaaatg
      181 tcatttttgg agcttttaaa ttttcttaat aagtatataa tagaatttgt gttaaaaact
      241 tagaaaaaga ggaattataa tgaacaataa gcagaaggga ataagtctag ttttaatagc
      301 gacagttttt tggggcataa tgggcataag cagtagaatc ctatatgaag caggattaag
      361 cacaatgttt atagctttta gtagaagttt tttatcttct atttgctttt ttatttggat
      421 aatgataact gatagaaaaa ttttaaagat agatccaaga ggtttagcta tttcagcatt
      481 atatggaata ggaacttttg cattatgttt tattggatat aacttgtccg tagaatatat
      541 acctatatct gtagccacag tattgatgtt tactaactca atatgggtaa caatctttgg
      601 ggtattattc tttggtgaaa agttcaatat gaaaaaggga tctgttatcc tattaacttt
      661 ggtaggatgc ataatggttt caaatatgtc tttaggacat tttaatatga gtgcaatagg
      721 aatagccgct ggattaggaa caggattttt atttgctttt caaataatat ttcctaaatt
      781 ttttagtggt tatagaaaag acacattgct tatttatagg tttatttaca gattttaaag
      841 aatcatttta tataattgta aatacaccca atattgggat ggtcatatta aatatattat
      901 ctataggtat tttaagtaca tttatatcta atacattcta tataaaatca actgaataca
      961 tagaggctag tgttaccagt atattagctt ctatggaacc agtactatca agtatatttg
     1021 ctttctttat ttttggagaa gtattaaatg gtaagcaaat agttggtgct atattaataa
     1081 ttatagctgc cataatatta gagttgaaat tagataaaga aagaataaac aatgtattta
     1141 agttgaaatt tataaatcaa aattaattag ctaataagtt ataatataaa ttttaaaaat
     1201 ccctgactta aaatgaatat tataaattat tgattaggct gtatctattt aggtgcagtc
     1261 cttttttata caataaaata tttcttaata atgaatttgg agcttttcgt gtaaatacca
     1321 gtaattggca tccgtttgga gttaggggta tagtcactgg aacagtttct caataaaaac
     1381 aatgataatt aatataggga ttttatttta gaattaatta aattaataga gttataaatt
     1441 tataaagt
//
LOCUS       DAEVGI010000282         1445 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEVGI010000282 DAEVGI010000000
VERSION     DAEVGI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.284"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(59..418)
                     /gene="rplT"
                     /locus_tag="K8M77_002551"
     CDS             complement(59..418)
                     /gene="rplT"
                     /locus_tag="K8M77_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBI7086980.1"
                     /translation="MARVKRAVNARKNHKKVLKLAKGYYGGKSKLFKTANESVIRALR
                     NAYVGRRLKKRDYRRLWIARINAATRMNGLSYSRFMNGMKLAGVDINRKMLSEIAIND
                     PKAFADLVELAKKHLNA"
     gene            complement(448..645)
                     /gene="rpmI"
                     /locus_tag="K8M77_002552"
     CDS             complement(448..645)
                     /gene="rpmI"
                     /locus_tag="K8M77_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBI7086981.1"
                     /translation="MPKMKTHRGAAKRFKKTGTGKLKRAHAFTSHILTKKSAKRKRNL
                     RKTGYVSTAQEKAMKKLLPYL"
     gene            complement(667..1188)
                     /locus_tag="K8M77_002553"
     CDS             complement(667..1188)
                     /locus_tag="K8M77_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBI7086982.1"
                     /translation="MNKISKNFAINQEIREKEVRLISSTGEQLGVVSGRDAQRMADEA
                     ELDLVMISPNAKPPVCKIMDYGKFIYEQSKKEKEAKKKQKVISVKEIRVSPTIEKHDL
                     EIKAKNAKKFLEAGDKVKITVRFRGREAEHSHVGVKILDNFLNQLEEVCSVEKPAKLE
                     GRNMIMILAPKKA"
     misc_feature    complement(1218..1337)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
BASE COUNT          402 a          272 c          168 g          603 t
ORIGIN      
        1 tgaataaaat taaacttata aaatataaaa aaaatgcgag ataaactcgc atttgatatt
       61 aagcatttaa gtgcttttta gctaattcaa ctaaatcagc aaatgctttt ggatcattta
      121 tagctatctc tgaaagcatt tttctgttta tatcaacacc agctaatttc attccgttca
      181 tgaatcttga atatgaaaga ccgttcattc ttgtagctgc gtttattcta gctatccata
      241 atcttctgta gtctctcttc tttaatcttc ttccaacgta agcatttctt aatgctctta
      301 ttactgattc gttagcagtt ttgaataact tgctttttcc accatagtat ccttttgcaa
      361 gttttaaaac ttttttatga tttttacgag catttaccgc tctcttaact cttgccattt
      421 tctagacctc ctactccgtg ttttatctta tagatatggt aataatttct tcattgcttt
      481 ttcttgtgca gttgaaacat aaccagtttt tcttaaattt ctttttctct tagcactttt
      541 ctttgttaag atatggctag tgaaagcgtg agctctcttt aactttccag ttcctgtttt
      601 tttgaatctt tttgctgcac ctctatgagt tttcattttt ggcattgtaa gtatcctcct
      661 ctcaagttac gcttttttag gagctaaaat cattatcatg tttctacctt ctaatttagc
      721 aggtttttct actgaacata cttcttctaa ttgatttaag aaattatcta atatcttaac
      781 accaacatgt gaatgctcag cttctcttcc tctaaatctt acagttattt tgactttatc
      841 tccagcttct aagaattttt tagcattctt agcttttatt tctaagtcat gcttttctat
      901 agttgggcta actcttatct ccttaacact gataaccttt tgcttctttt ttgcttcttt
      961 ttctttttta gattgctcgt aaataaattt accgtaatcc attattttgc aaactggtgg
     1021 tttagcattt ggcgaaatca taactaagtc taactcagcc tcatctgcca ttctttgtgc
     1081 atctcttcca gatacaacac caagttgctc tcctgtgcta gaaattaatc taacctcttt
     1141 ttctcttatt tcttgattga ttgcaaaatt tttactaatc ttgttcacct ccgatagaat
     1201 ttagccaaca caaatatata aataaaaaag gacggtttta agccgtcctc tattaccaaa
     1261 atcaaatgta ataaaattca tgaccaaact agcattaata gctgtaaggt gagaaacggc
     1321 tgtttcttct tgacatcagt tatcttatca ttattctttt ttattgtcaa cataattcat
     1381 aaatttcttt aaataaaata atagttttat atctttaaaa cttataattt taagatttat
     1441 tacaa
//
LOCUS       DAEVGI010000283         1428 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEVGI010000283 DAEVGI010000000
VERSION     DAEVGI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.285"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            202..1383
                     /locus_tag="K8M77_002554"
     CDS             202..1383
                     /locus_tag="K8M77_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI7086983.1"
                     /translation="MKYIIGVVGLIVILALAWIGSSDKKNIKYKPIFLMLILQFVLGF
                     ILLNTQVGNYLVGSIANGFQLLLDCAGDGVNFVFGGLVNNNQFSFFISVLLPIIFISA
                     LIGILQYLKILPFVIKYIGLVLSKINGMGKLESYNAVASAILGQSEVFISVKKELGLL
                     PKHRLYTLCASAMSTVSMSIVGSYMVLLQPRYVVTAIVLNLFGGFIIASVINPYSVSK
                     EEDILVVEDEKKQTFFEVLGEYILDGFKVAVIVAAMLVGFVAIIAVINMIFRAIFGMS
                     FQDILGYVFSPFAFLMGVPLNESIQAASVMATKLVSNEFVAMNLLSSGNLNLSARSIG
                     IVSVFLISFANFSSIGIISGAVKGLNQEQGNVVARFGLKLLYGATLVSMLTATIVGLI
                     I"
BASE COUNT          498 a          145 c          244 g          541 t
ORIGIN      
        1 tacatatgtt aaaaaaaaat ttataattga aatgtttata aatatgtcga aaagatgtac
       61 gaattaatat ttttttaaaa aataaaactg aataaggatt tttttgggaa atattaaaaa
      121 caatctacta aaggtgtatt tattgtacca tatgcaaaag acaataatat taaatttatg
      181 gtatataaca aggaggataa aatgaaatat attattggag ttgtaggatt aattgttatt
      241 ttagcacttg cgtggattgg aagtagtgat aagaaaaata taaaatataa gcctatattt
      301 ttaatgctta ttttacaatt cgtattagga tttatacttt taaatactca agttggaaat
      361 tatttagttg gaagtatagc aaatgggttt caacttcttc tagattgtgc tggagatggg
      421 gtaaattttg tatttggtgg attagttaat aataatcaat tttcattctt tattagtgtt
      481 cttttaccaa taatctttat ttctgcctta attggtattt tacaatactt gaaaatactt
      541 ccttttgtaa ttaaatacat aggtttagtt ttaagtaaaa ttaatggcat gggtaaatta
      601 gagtcttata atgctgtggc atcagctata cttggtcaat cagaagtatt tatttctgta
      661 aaaaaagaac ttggtttatt acctaaacat agattatata ctttgtgtgc atcagcaatg
      721 tcaacagtat ctatgtctat agttggttct tacatggttt tattacaacc tagatatgtt
      781 gtaacagcaa ttgttcttaa tttatttggt ggattcatta tagcatcagt tataaatcca
      841 tattctgtat ctaaagaaga ggatatttta gtagttgaag acgaaaagaa acaaacattc
      901 tttgaagtgc ttggtgaata tattttagat ggatttaagg tagcagttat agtagcagct
      961 atgttagttg gatttgtagc aattattgct gtaataaaca tgatatttag agcaatcttt
     1021 ggaatgagct tccaagatat actaggatac gtattctcac cttttgcatt cttaatgggt
     1081 gtacctttaa atgagtcaat acaagcagcc agtgtaatgg caacaaagct agtatctaat
     1141 gagtttgttg caatgaattt attatcaagt ggaaatttaa atttatcagc aagatcaatt
     1201 ggtattgttt ctgtattttt aatttctttt gcaaatttct catcaattgg aattatttca
     1261 ggagcagtaa aagggcttaa ccaagaacaa ggaaatgtgg ttgcacgttt cggacttaaa
     1321 ttactatatg gtgcaacatt agtaagtatg ttaacagcaa ctattgttgg attaattata
     1381 taaataaatt aactttgttt tgtttatata gtaaaatgta gagagaaa
//
LOCUS       DAEVGI010000284         1400 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEVGI010000284 DAEVGI010000000
VERSION     DAEVGI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.286"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..486
                     /locus_tag="K8M77_002555"
     CDS             <1..486
                     /locus_tag="K8M77_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7086984.1"
                     /translation="ELILFLYRYYLCSFTEDIEKALNDVLELNSMFRQTLSEREVIRA
                     TRSAERCYLDKNKKYKYKNETLIELLEITDEEQKYMTIIISKEEYKRRDRVYQKKRYQ
                     EKLKVQGKLSEKQKIYNRRKKIKALLDKGLKQKDICLQLNISKLTYIRDRKFLKEQGL
                     I"
BASE COUNT          582 a          118 c          222 g          478 t
ORIGIN      
        1 gagcttatac tatttttata tagatattac ctttgtagtt ttacagaaga cattgaaaag
       61 gcgctaaatg atgttttaga actaaatagt atgtttagac aaactttaag tgaaagagaa
      121 gttataagag caactaggag tgctgaaaga tgttatttag ataaaaataa gaaatataag
      181 tataagaatg aaactcttat agagttatta gaaattacag acgaagaaca aaaatatatg
      241 acaataataa tttctaagga agaatataag agaagagata gagtttatca gaaaaaaaga
      301 tatcaagaga aattaaaggt tcaaggtaag ttaagtgaaa aacaaaaaat atataaccgt
      361 agaaaaaaaa taaaagccct tttagataaa ggacttaaac aaaaagatat atgcttgcaa
      421 ttaaatatat ctaaattaac gtatattcgt gatagaaagt tcttaaaaga acaaggttta
      481 atatgacctt gttctttttg tattatagga tgttgtttga cataagtcaa atatattttt
      541 attagttgtc aataggtatc aaattttctc ggcttgtatt atgagtactt agtacttgtt
      601 ctcctttttg tataaaaata cctatctgat aattgcaaga atagttccaa aggaacgtca
      661 gagagagtac ccctttattt tttggcgtgg ctataaaagt aaaaaaatat tttatataaa
      721 taattaaata ttaatagggc gtaaagtggt agcatatgtg tcgtaaagtg ggattaaagt
      781 gggattttat agggcgtgtt tatggcttag agtgggataa ttggaagttg atttgatatc
      841 attaaaatac taggctttgc ctagactata accttgggtg atagcaaatt ttccttatgc
      901 tctttttaaa taaattgtta ttattaatat aagtagaaat ttacaataag aggtgtttaa
      961 ctatgggttt taaaaaaata tatatgctta tattaataat tatattgatt ctaattatta
     1021 taggttttat ctaaataaac aaaacctata ataatcagaa ctacccaaaa tatattacag
     1081 aaaaaaattt agaagatatt attgataaaa atgttgccac aaccatatta aaaaaagaaa
     1141 aaataagtga taaagaaaaa actttttgtg attttaagat tatagatatt attaaaaaaa
     1201 ataatatcta taatgtttat ctttggatat atgctcaaaa tttttctgtt gaaaataata
     1261 ttttaattaa aggagcaggt ggtgagtttc ctgttatttt gaaattaaaa aaagaaaaaa
     1321 atgattttaa atttattaat tatggtgaat caattaaaat ttttccgaaa gaagttagaa
     1381 aaaaagctaa tgaaagttat
//
LOCUS       DAEVGI010000285         1397 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEVGI010000285 DAEVGI010000000
VERSION     DAEVGI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.287"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            44..1342
                     /locus_tag="K8M77_002556"
     CDS             44..1342
                     /locus_tag="K8M77_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBI7086985.1"
                     /translation="MDIEKFLREIKEKIINYRRDFHKYPESAWKEFRTSSLIGKYLIE
                     MGYEIKIGKEIISEEHRLDVSNELDLKKSYEKSLNQGAYKELSDYMIGGYTGVVGILQ
                     NGEGPTIALRFDIDGVKVLECENNEHFPFKNGFSSLNRGIMHACGHDGHIAIGLGVAE
                     GIIRFKEYLSGTIKLIFQPGEESVCGGNPMAQSGILDDVDYLLSGHIGIKARKSGEII
                     CGTKGFLATSKINAEFTGKSSHAAVAPEKGHNALLSASTAVLNLDAIPRSGKGVTRIN
                     VGKLIAGSGRNVIPGKAFMEVETRGETTELNEYMESYATRILKASADMHDNHVKISCL
                     GRSISGSSDRELIDIIKSEAKNINDYNNIIDEEDFGASEDICYMMKRVQKNGGKASYI
                     MFGSELKDEHHSIRFDFNEEDMFPAINLYLRVVMNLCNKR"
BASE COUNT          549 a          117 c          296 g          435 t
ORIGIN      
        1 agtatatgta tttttaaaaa ttatttaatg tggaggatta aaattggata ttgagaagtt
       61 tttaagggag attaaagaaa aaataattaa ttatagaagg gattttcata aatatcctga
      121 aagtgcatgg aaagaattta gaacatcttc tttaattgga aaatatttaa tagaaatggg
      181 ttatgaaata aaaataggaa aagaaataat aagtgaagag catagattag atgtatcaaa
      241 tgaacttgat ttaaagaaaa gttatgaaaa atcattaaat caaggtgcct ataaagaatt
      301 aagtgattat atgattgggg gatacactgg agtagttggg attttacaaa atggagaggg
      361 tccaactatt gctttaagat ttgatataga tggagtaaag gtccttgaat gtgaaaataa
      421 tgagcatttt ccttttaaaa atggtttttc ctctttaaat agagggatta tgcatgcctg
      481 tggacatgat ggtcatatag ctattggtct tggagttgca gaagggatta ttagatttaa
      541 ggaatattta agtggaacaa taaaacttat ttttcaacct ggagaagaaa gtgtatgtgg
      601 tggaaatcca atggctcaat caggaatctt agatgatgta gattatttat taagtggaca
      661 cataggaata aaggcaagga agtcaggaga aataatctgt ggaacaaagg gatttttagc
      721 aacatctaaa attaatgctg aatttacagg gaagtcttcc catgccgcag ttgcacctga
      781 aaaagggcat aatgctttgc tttctgcatc tacagcagta ttaaacttag atgctatacc
      841 aagaagtggt aaaggcgtaa caagaataaa tgtaggaaag ttaattgcag ggagtggtag
      901 aaatgtaatt cctggaaaag cttttatgga agtagaaact agaggagaaa ctacagaatt
      961 aaatgaatat atggagagtt atgcaactag aatcttaaaa gcttctgcag atatgcatga
     1021 taaccatgtg aaaatatctt gtttaggaag atctataagt ggtagtagtg atagagagtt
     1081 aattgatatt attaaatcgg aggcaaaaaa tataaatgat tataacaata taatagatga
     1141 ggaagatttt ggtgctagtg aagatatatg ttatatgatg aaaagagtac agaaaaatgg
     1201 aggaaaagca tcttatataa tgtttggaag tgagttaaag gatgaacatc attctattag
     1261 gtttgatttt aatgaagaag atatgtttcc agcaataaat ttatatttaa gagtagtaat
     1321 gaatctttgc aataaaagat aataaaattt ttaaaagatg aattatattt aatagttatt
     1381 ctatgtatat atatgaa
//
LOCUS       DAEVGI010000286         1389 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEVGI010000286 DAEVGI010000000
VERSION     DAEVGI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.288"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            274..1287
                     /locus_tag="K8M77_002557"
     CDS             274..1287
                     /locus_tag="K8M77_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF348 domain-containing protein"
                     /protein_id="HBI7086986.1"
                     /translation="MVNIKKLFSKENRNKTLALGLTGLVLVGGTVVFSMRKTLNVVVN
                     GERTEIVTYKGTVQGALHDNGITLAPKDKVTPSLESKISKNETITINKAVNVKVKTED
                     GEKEIVSAEDNVENMLKSEGISFDDDDKILPDKKESLKDGMNVEVVKVDVKKVTEVHP
                     IEFTTEVKKDESKPQTYTEVLNDGQDGEKKVTRELVYENGKEVSNNVIQELVVKEPVN
                     KEVVKGTKETQTLSRGGESINFKKKLSVKSTAYNHPLGSSEAYTASGMHVLRDPNGYS
                     TIAVDPSVIPLGTKLYVEGYGYAIAADTGGAIKGNRVDLFFNTEAEASNWGVRNLDVY
                     ILN"
BASE COUNT          566 a          161 c          284 g          378 t
ORIGIN      
        1 aattaatagc ataaaactat gcattatttg aagaaagaag aagtatagta gagcatttat
       61 ggacataaat aaggtttttg cagcctatat ctaagaattt atagaaatag taagaatatg
      121 agtaaaatag cttgtttgat taataattac aaataggtta caataagtaa tgtattttac
      181 ttaaaaagtg taaaatccct tctgacatac agaaaagcgt ataactttcg catggaagcg
      241 taaaactttt acttgccaga aggaggagat aatatggtaa atattaaaaa gttgttttca
      301 aaggaaaata gaaataaaac tttagcctta ggactgacgg ggttagttct agttggagga
      361 acagtagtat tttcaatgag aaaaactctt aacgtagtag ttaatggaga gagaacggag
      421 attgttacct ataaaggtac agtacaaggg gctttacacg ataatggaat aacattagca
      481 cctaaagaca aagtaacacc ttcattagaa agtaaaatat caaaaaatga gacaatcact
      541 ataaacaagg ccgttaatgt gaaagtaaaa acagaagatg gcgaaaaaga gattgttagt
      601 gcggaagata atgtagagaa tatgttaaaa tcagaaggaa tatcttttga tgatgatgat
      661 aagatactac cagataaaaa agaaagttta aaagatggaa tgaacgttga agtggtaaaa
      721 gttgatgtaa aaaaagttac agaagttcat cctatagaat ttacaacaga ggttaaaaag
      781 gatgaaagta aaccacaaac atatactgaa gtattaaatg atggacaaga tggagagaaa
      841 aaagtaactc gtgaacttgt ttatgaaaat ggaaaagaag taagtaataa cgttatacag
      901 gaattagttg taaaagagcc tgttaataag gaagttgtta aaggtacaaa ggaaactcaa
      961 actttaagta gaggtggaga aagcataaac tttaagaaaa aacttagtgt taaatccaca
     1021 gcttataatc atccattagg ttcttcagaa gcatatactg cgtcaggaat gcatgtttta
     1081 agagatccaa atggatatag tacaatagcc gttgatccaa gtgtaattcc tttaggaaca
     1141 aaattatatg ttgaaggata tggatatgct attgcagctg atacaggtgg tgctataaaa
     1201 ggaaacaggg tagatctttt ctttaacaca gaggcagaag catctaactg gggcgttaga
     1261 aacttagacg tttatatatt aaattaacta cgatgaaggg acaggcttta tgcctgtctt
     1321 tttatatgcc tttggcaaga acacccgtga tttcaatcat aggatgaatt gccacgacat
     1381 atgcaaaag
//
LOCUS       DAEVGI010000287         1387 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.289, whole genome shotgun sequence.
ACCESSION   DAEVGI010000287 DAEVGI010000000
VERSION     DAEVGI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.289"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            388..1386
                     /locus_tag="K8M77_002558"
     CDS             388..1386
                     /locus_tag="K8M77_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBI7086987.1"
                     /translation="MIKLVCYGVRETEVPYFHKLNEEFGYDLTLVEKNLNHENVEEAI
                     GAEAIMVRGNCMADRQNLELLKSKGLKYVLTRTVGFDHVDLEAAKDLGLQVARVPGYS
                     PNAIGELAVSLAMMLLRHTAYTTHRTSNKNFVVDGFMFSKEIRNCTVGILGAGRIGLT
                     TAKLFKGLGAKVVAYDVFQSDAAKEIVEFMPLDEVLKVSDVISVHMPYIKGQNYHMIN
                     EEFISKMKNDAIIINTARGELQDIEAIVKALEEGRLGGFGTDVLEGESAVFFKNLEGQ
                     KLENESYEKLISLYPRALVTPHMGSYTDEALRNMIGISYENLKEYLEKNTCKNAIA"
BASE COUNT          550 a          152 c          257 g          428 t
ORIGIN      
        1 attacaagac tccagaagaa ttatttgaaa tacacttaga tgaaatatat tcattatatt
       61 aattagttct taaatcataa ttaaggacta gttcaatttg atattgcaat ttagacatta
      121 aatatagttc aataattatt gaatttcgtt aagaatttct attctttatt ccatagattc
      181 agctaaagct caaaaacttg cttcgcttca aacagttttc gctttttaac gctgaatcta
      241 ttccataaaa aatgaaattc taagcactca ttcaaatatt atttcactta tatttaatat
      301 gaaaaattta attagaaatt taaattaaaa ggataaatag agatttgaat aaagaagaaa
      361 agatggggaa aagaggaggt ttttcgtatg ataaaattag tatgttatgg agttagagaa
      421 acagaggtac catacttcca taagttaaat gaagagttcg gatatgattt aactttagtt
      481 gaaaaaaact taaaccatga aaatgtagag gaagctatag gggctgaggc tataatggta
      541 agaggaaact gtatggctga tagacaaaat ttagaactat taaagagtaa gggtcttaaa
      601 tatgttttaa ctagaactgt tggatttgac catgttgatt tagaagctgc taaggattta
      661 ggattacaag ttgctagagt accaggttac tcaccaaatg ctataggaga attagctgtg
      721 agtttagcta tgatgctttt aagacataca gcatacacta ctcatagaac aagcaataaa
      781 aactttgtag tagatggatt tatgtttagt aaagaaataa gaaactgtac agttggaata
      841 ttaggggcag gtagaatcgg attaacaact gctaaattat ttaaaggatt aggagctaag
      901 gttgtagcat atgatgtatt ccaaagtgat gccgctaagg aaatagttga atttatgcct
      961 ttagatgaag ttttaaaagt ttctgatgta atatcagtac atatgccata tataaaaggg
     1021 caaaattatc acatgataaa tgaagaattc atatcaaaaa tgaaaaatga tgctattata
     1081 atcaatactg caagaggaga attacaagat atagaggcta tagtaaaagc tcttgaagaa
     1141 ggaagattag gcggatttgg aactgacgtt ttagaagggg aatcagcggt atttttcaaa
     1201 aacttagagg gacaaaagtt agaaaatgag tcatatgaaa aattaataag cttataccca
     1261 agagctttag taactcctca tatgggttca tatacagacg aggctttaag aaatatgata
     1321 ggaatatcat atgaaaactt aaaggagtat ttagaaaaaa atacttgtaa aaatgcaata
     1381 gcataat
//
LOCUS       DAEVGI010000288         1386 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEVGI010000288 DAEVGI010000000
VERSION     DAEVGI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.290"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            93..353
                     /locus_tag="K8M77_002559"
     CDS             93..353
                     /locus_tag="K8M77_002559"
                     /inference="COORDINATES: protein motif:HMM:NF027249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI7086988.1"
                     /translation="MKKIYSYPSILTKENDGITVTFPNFKGCISCGYTFEEAIKNGKQ
                     ALGLHINGLIEDNEVLSDPKEIKNIKLYENQKIEFIEVEIEE"
BASE COUNT          562 a          108 c          194 g          522 t
ORIGIN      
        1 attcaagaaa ttaaatttaa tttagaaaaa agaaaattaa aaaataaaaa aaatatattt
       61 aaaaatattt ttaatttaag gagataatta atatgaaaaa aatatatagt tatccaagta
      121 ttttaacaaa agaaaatgat ggaataacag taactttccc gaattttaaa gggtgtattt
      181 cttgtggcta cacttttgaa gaagctataa aaaatggaaa acaggcttta ggtttacata
      241 taaatggctt aatagaagat aatgaagtgc tgtcagatcc aaaagaaata aaaaatataa
      301 aattatatga aaatcaaaag atagagttta ttgaggttga aatagaagaa tagcaagtat
      361 agattttgtc atgacacttt aaagttgtta tatcaaaatc atcattgtta ggaataatcc
      421 taagacctat ttaacataat aaaattaaat ttaattgtgg ttacaaggtt taaaaatgta
      481 gctacaatgt tttttagcta tgattttaac agtatctagg aagcttttta agttttttat
      541 gtgactacaa gaaaaatttt tgtagctaca taaagtttta tataaatttg ataaaacttt
      601 gaaaatatta taaaattaat gtattagatt attacaatat aagttaaaaa agttttttac
      661 ttatattcaa cttttttaaa ctataggttt aagattactt caattttttt aagctaaagc
      721 ttaggattac ttaaatttta aaatagcctt ttaaaggcta aggataacat aaaaaaataa
      781 atattagctt ataaaagttt ataagcaaga ttaataacaa atagataaat ctaaaaattt
      841 taaaaagaaa ataaatatta gtatttacca gtaaacctgg tgtttaatat gtcttttata
      901 gtggtgtagc cacttactaa ttatttagtt ttaaatttta aaatttggta gatttttttt
      961 atttgttttt ttatataaat ttagtttttt aaaaatttta ttttaaaaat ttttaaaaaa
     1021 caggatacca aattaatttt ttgcatttgt aaaatgcaaa aaataatatt gctgcggagc
     1081 tgcaatatta ttttagtgtt tattttttaa tagacatttt gtaggtttct atttatttag
     1141 aaatttgaag aatcgacggt tattgcttaa aagctcaata acggatttta tttagttctc
     1201 ctagtgttgg agaattgtga ttgaaaggaa aaagctttta ttattttttt atttttagta
     1261 tataccatgt cggcattaac aggcggacat agggagcctg ttaatgccga acataagtaa
     1321 atatgaggtt ttagaatgga ttggagttat ttttatgagt aaaaagaaaa atagaaatat
     1381 taacgg
//
LOCUS       DAEVGI010000289         1382 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEVGI010000289 DAEVGI010000000
VERSION     DAEVGI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.291"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..525
                     /locus_tag="K8M77_002560"
     CDS             <1..525
                     /locus_tag="K8M77_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBI7086989.1"
                     /translation="FKVKDIEGNEVSLGEYKGKVLLIVNTATGCGFTPQYEGLEMLYK
                     KYHDKGFEILDFPCNQFFEQAPGSNEEIAGFCKLNYGTTFKTFAKVEVNGENACELYK
                     FLKKEAPMAKEDETSLGFYDKLKGLGFTTEGEEIKWNFTKFLIDKNGEVIARFAPTFE
                     PEKLDELIEELLEE"
     gene            complement(642..>1382)
                     /locus_tag="K8M77_002561"
     CDS             complement(642..>1382)
                     /locus_tag="K8M77_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBI7086990.1"
                     /translation="QGPIGPQGEQGPQGERGFTGPQGPIGPQGNQGPIGPQGEQGPQG
                     ATGPQGPQGPVGPQGNQGPIGPQGPVGPQGPQGPQGQPGVNFNDTLLVSSSSLSSQNV
                     GSNGIFTYNIQNPNGSTFTAITANIANGTFTINEPGRYLFIWSFNLDNTNNTTANTIV
                     SLFRNGFRIFLSGTPRVAPGEIGVVNGSIAVNANAGDVFALVNNSTRNVLSQIISSPI
                     SVTPAILGESTGINSGIGSWVQIVKVSD"
BASE COUNT          484 a          196 c          258 g          444 t
ORIGIN      
        1 tttaaagtta aggatattga aggaaatgag gtttctttag gggagtataa aggaaaggtt
       61 cttttaatag ttaatactgc aacaggatgt ggatttactc ctcaatatga aggattagaa
      121 atgctttata aaaaatacca tgataaagga tttgaaatat tggattttcc atgtaatcag
      181 ttctttgagc aagcaccagg aagtaatgaa gaaatagctg gattttgtaa gttaaattat
      241 ggaactacat ttaagacctt tgcaaaagta gaggttaatg gagaaaatgc ttgtgaatta
      301 tacaaatttt taaaaaaaga agctccaatg gcaaaggaag atgaaacatc tttaggattt
      361 tatgataaat taaaaggctt aggatttact acagaaggag aagaaataaa gtggaatttc
      421 actaagtttt taatagataa aaatggtgaa gttattgcta gatttgcacc cacatttgaa
      481 cctgaaaagt tagatgaatt aatagaagaa ttattagaag aataatacag tttttaagga
      541 aaagacctat aattagattt ttaatactaa tcataggtct tttaatgttt taatgtgaat
      601 ttaataaatt aaagttaact aattatttag tgaatttctt attaatcaga tactttaact
      661 atttgaaccc aagatcctat tcctgaattt atccctgtag attctcctaa gatagctgga
      721 gttacagaaa ttggtgaaga tattatttgt gataaaacgt ttcttgtaga attattaact
      781 aaagcaaata catcaccagc attagcattt acggcaatac ttccatttac tacgcctatt
      841 tcaccaggag ctactctagg agtaccagat aaaaagattc taaaaccatt tctaaataaa
      901 gatactatag tattagctgt agtattattt gtgttatcta aattaaatga ccaaataaat
      961 aaatatcttc caggttcatt tattgtaaac gttccgtttg ctatattggc agttattgct
     1021 gtaaaagttg aaccattagg attttggata ttataggtga atataccatt agaaccaaca
     1081 ttttgtgaag ataatgaaga actactaact aataaggtat cgttaaaatt aactccaggt
     1141 tgcccttgag gaccttgagg accttgagga ccaacaggac cttgaggacc aatagggcct
     1201 tgatttcctt gaggaccaac gggaccttga ggaccttgtg gtcctgtagc gccctgaggt
     1261 ccttgttcac cttggggacc aataggacct tgatttcctt gaggaccaat aggaccttga
     1321 gggccagtaa atcctctttc accttgaggt ccttgttcac cttgaggacc aataggacct
     1381 tg
//
LOCUS       DAEVGI010000290         1355 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEVGI010000290 DAEVGI010000000
VERSION     DAEVGI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.292"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(189..386)
                     /locus_tag="K8M77_002562"
     CDS             complement(189..386)
                     /locus_tag="K8M77_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBI7086991.1"
                     /translation="MDSEMFKLIATQGAFALLFSYLLFYVLKENSKREENYQSIIKEL
                     TELLPSIKNDVEDIKNKLNSN"
     gene            complement(437..1003)
                     /locus_tag="K8M77_002563"
     CDS             complement(437..1003)
                     /locus_tag="K8M77_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBI7086992.1"
                     /translation="MQELYEKIKLCKLGNKEALQEVVNIFEKTIMKELFKFKKENSTI
                     FMNEYDFQDKKSEITLNVLNAIKNMPIESFEYKTDYSVKKYINITILNKLNEIKTKEF
                     NKTENEYKYEFDFSFFKSSDFNEPNTDIFVHDLIDKLSEKERKIIKYKYIDGKSDVEI
                     GELLNCSRQYVNQTKNRALKNLKKFLDD"
     gene            1242..>1355
                     /locus_tag="K8M77_002564"
     CDS             1242..>1355
                     /locus_tag="K8M77_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7086993.1"
                     /translation="MKIAIRGGHNFLSKGACGLIDETTENRKVYKSVIKNLI"
BASE COUNT          472 a          183 c          118 g          582 t
ORIGIN      
        1 gttggcttac ttccaactga atctcctgga tttacacttg gtataaatct cattatcatt
       61 tttaaaacct cactttttat ttttaaaatc aagcttgaat ttgttttaca ctatataaag
      121 agtgagattt tttaattttg taaataagaa aattaaaaaa agctgatttt tttatcagct
      181 tttttattct aattactatt taatttattt tttatatctt ctacatcatt ttttatactt
      241 ggtaacaact ctgtaagttc tttaataata ctctgataat tttcttctct tttactattt
      301 tcctttagta cataaaataa taagtatgaa aatagtaagg caaatgcacc ttgagttgct
      361 ataagcttaa acatttctga atccacaata aacctcctat taaaatttta tttcaagctt
      421 gatattttct aactaattaa tcatctaaaa atttctttag attttttaat gctctattct
      481 tagtttgatt tacatattgt ctagaacaat taagcaattc tcctatttca acatcacttt
      541 ttccatctat gtatttatac ttaattattt ttctttcttt ttcagaaagc ttgtctatta
      601 aatcatgtac aaatatatct gtattaggct cattaaaatc actagactta aaaaagctaa
      661 aatcgaactc atacttatat tcattttctg tcttattaaa ttcttttgtt ttaatctcat
      721 tcaatttatt taaaattgtt atatttatat atttttttac agaatagtca gttttatatt
      781 caaaactttc aattggcata ttttttattg catttaaaac atttaatgta atttcactct
      841 ttttatcttg aaagtcatac tcattcataa aaatagtact attttctttt ttaaatttaa
      901 ataattcttt cattatagtt ttctcaaata tattaacaac ttcttgtaaa gcctctttgt
      961 ttcctaattt acataacttt atcttttcgt ataattcttg cataaaattc tcctcataat
     1021 ctcttttatt ttttaaagag aaaaaatgag gaaaagtgta aatctccaaa ataagctttt
     1081 aaaaaaacgt aacgttccgt tctataattt tacaaaatat acatttttgt ttcaaaacac
     1141 ccaatatatt ttatttttat gtttacaaaa caaaagtcta tcaatctttt aaatatataa
     1201 gcttatttaa ataaaatttt taaaaggaaa ggaagaaaaa tatgaaaata gctatacgtg
     1261 gtggtcacaa ttttttatca aagggagctt gtggattaat agatgaaact actgaaaata
     1321 gaaaggttta taagtctgta attaaaaact taatt
//
LOCUS       DAEVGI010000291         1338 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEVGI010000291 DAEVGI010000000
VERSION     DAEVGI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.293"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(47..1231)
                     /locus_tag="K8M77_002565"
     CDS             complement(47..1231)
                     /locus_tag="K8M77_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI7086994.1"
                     /translation="MEINKNIVKSIAEHLSDKPFDLEYWDGEIIKYGEGEPEFKLIIK
                     NFPSKKELLSDPSVALGEAYMKGDIDIEGDLQKFFESIIRNKDSFMNKNTVFRLASKI
                     KAPSLMKSKKDIAHHYDIGNDFYSLWLDKTMSYSCGYFKNPTDTLYDAQMNKIHHILK
                     KLNLKEGQHLLDIGCGWGYLIIEAAKLYKVKALGITLSEEQFKKAKERIKQEGLEDLV
                     DVQLMDYRNLEKSNLEFDRIVSVGMAEHVGHANLPLFFKNVDSVLKESGLFLLHNITN
                     LVETEGNKWITTYIFPGGYLPTLREELNIAADINFRTLDVESLRLHYMKTLEEWCKNF
                     MNHLDEERHMFDDEFLRMWHLYLATCAAAFHYWDIDIHQILFSKGINNTLPMTRKYLY
                     ED"
BASE COUNT          432 a          218 c          157 g          531 t
ORIGIN      
        1 caaaacctta taataagact taattgatac aagaccttaa tattaattag tcttcatata
       61 aatattttct tgtcatagga agagtgttgt ttattccttt tgagaatagg atttgatgta
      121 tgtcaatatc ccaatagtgg aatgcagctg cacaagtagc aaggtataaa tgccacattc
      181 ttaagaattc atcatcaaac atatgtcttt cttcatctaa atgattcatg aagtttttac
      241 accattcttc taatgttttc atataatgaa gtcttaaact ttctacatct aatgtacgga
      301 aattaatatc tgctgcaata ttaagttctt ctcttaatgt tggaaggtat ccacctggga
      361 atatgtatgt tgtgatccat ttgtttcctt ctgtctctac aaggttagtt atgttatgta
      421 ataggaataa tccactttct tttaatacag aatcaacatt tttgaagaat aaaggtaagt
      481 tagcgtgacc aacatgttct gccataccaa cactaacaat tctatcaaac tctaagttgg
      541 atttttctaa gtttctataa tccattaatt gaacatcgac taaatcttca agtccttctt
      601 gttttattct ttctttagct ttcttaaatt gttcttcact aagagttata cctaaagctt
      661 ttaccttata aagtttagca gcttctatta ttaagtaacc ccaaccacaa cctatatcta
      721 ataaatgttg accttctttt aaattaagtt tctttaatat gtgatgtatt ttattcatct
      781 gggcatcata aagtgtatct gttggatttt taaaatatcc acatgaataa ctcattgttt
      841 tatctaacca taaactataa aaatcattac ctatatcata gtgatgtgct atatcttttt
      901 ttgatttcat taaagatgga gcttttattt tagaagcaag tctaaatact gtatttttat
      961 tcataaagct atctttgtta cgaataatac tttcaaagaa tttttgaagg tctccttcta
     1021 tatcaatatc acctttcatg taggcttcac ctaaagctac actaggatct gataaaagtt
     1081 cttttttact tggaaaattt tttataataa gcttgaattc aggttctcct tcaccatact
     1141 taattatttc accatcccaa tattctaagt caaaaggttt atctgaaaga tgttcagcta
     1201 ttgatttgac tatatttttg ttaatttcca taaaaaaaat cacctcattt aatatttata
     1261 tttttatcat actccatatt tgttaaaaaa tctttcgaaa aatgcacttt aattccttaa
     1321 atttactgaa tctaatga
//
LOCUS       DAEVGI010000292         1328 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEVGI010000292 DAEVGI010000000
VERSION     DAEVGI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.294"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(189..692)
                     /locus_tag="K8M77_002566"
     CDS             complement(189..692)
                     /locus_tag="K8M77_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBI7086995.1"
                     /translation="MSYRIKQFLWSISANFKELDYSYVRSILNDYEFSLFQRLKKGEQ
                     LHSIKVSKDCVNLAKSKGINSESELKIFSKLGLLHDIGKLYYPLNIMTKSFLVLGKKM
                     SKNRISKFQNIKPIYIYYNHGDKAFDYLREDDYDKEFIEAIRGHHSIKSSENILLCIL
                     KEADDMN"
     gene            complement(965..1040)
                     /locus_tag="K8M77_002567"
     tRNA            complement(965..1040)
                     /locus_tag="K8M77_002567"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1005..1007),aa:Ala,seq:tgc)
     gene            complement(1047..1123)
                     /locus_tag="K8M77_002568"
     tRNA            complement(1047..1123)
                     /locus_tag="K8M77_002568"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1087..1089),aa:Ile,seq:cat)
     gene            complement(1129..1247)
                     /gene="rrf"
                     /locus_tag="K8M77_002569"
     rRNA            complement(1129..1247)
                     /gene="rrf"
                     /locus_tag="K8M77_002569"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          457 a          223 c          149 g          499 t
ORIGIN      
        1 tataaaatat ttttagatta tatcttattt tctagacata aaatttatat gaagtattat
       61 ccttataatt aaagttaaag taaattattt aactccacag aatataaatt gaaattattt
      121 caacataatt tatactgaat attagcttta ataaaattta tttaaaaact atgcacctat
      181 tattaaaatt aattcatatc atcagcttct tttaatatac ataacaatat gttttcacta
      241 gatttaatag agtgatgtcc tctaattgct tctataaact ccttatcata atcatcttcc
      301 cttaaataat caaatgcctt atcaccatga ttataataaa tatatattgg ttttatattt
      361 tgaaatttac ttattctatt tttagacatt tttttaccta acactaaaaa tgactttgtc
      421 attatattta aaggataata taattttcct atgtcatgta ataatcctag cttagaaaaa
      481 atttttaatt cactctctga atttatccct ttactcttag ctaaattaac acaatcctta
      541 gaaaccttta tggaatgtaa ctgttctcct tttttcaatc tctgaaataa gctaaactca
      601 taatcattta agattgatct tacataacta taatctaact ctttaaaatt tgcagaaata
      661 gaccataaaa attgctttat tctatacgac atctttaggt attcaccttc tattctcatt
      721 aaagatatta atactgtaaa ttataccata taaagataat tattatatat tatctttccg
      781 ttaaaatatt tatccactaa atactccata tatgtagata atttatttgt tttgtgtgga
      841 taacttgtta ttctttttaa aattttgtgg aaaactttta agattagtta aatcaataga
      901 ttcattttag atttgagtat atttaagaaa aataaaaaaa ctctaaacat ttgtttagag
      961 tttctggtgg aggtaaggag attcgaactc ctgaccccct gcgtgcaagg caggtgctct
     1021 cccaactgag ctataccccc aaaaaatggt ggaccttcag ggactcgaac cccggaccta
     1081 ccggttatga gccggttgct ctaaccaact gagctaaaga tccatattac ctggcaacca
     1141 cctactctcc cacacagtct cccgtgcagt accatcggcc tcaataggct taaccttcgt
     1201 gttcggaatg gaaaacgggt gttaccctaa agagcatcat caccagatga catgtatatg
     1261 ttatcaaatt tatgataatt tgtcaacata tttttgaaag tttttgttct ttcaaaattg
     1321 cacatatt
//
LOCUS       DAEVGI010000293         1316 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEVGI010000293 DAEVGI010000000
VERSION     DAEVGI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.295"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>1316
                     /locus_tag="K8M77_002570"
     rRNA            <1..>1316
                     /locus_tag="K8M77_002570"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          342 a          296 c          413 g          265 t
ORIGIN      
        1 acgcaggagg atagtgtgtg ctagctattg gatgctagtc taagcgttta ggcagtgaga
       61 gtaggcaaat ccgctctcat aatgctgagg cgtgatgggg aaggttctac ggaaccgaag
      121 tacatgattc cacgctgcca agaaaagcgt ctagagagag aagtagtgcc cgtaccgcaa
      181 accgacacag gtaggtgagg agagaatcct aaggccggcg gaagaattac agttaaggaa
      241 ctcggcaaat tgaccccgta agttcgcgag aaggggtgcc tacgagagta ggccgcagag
      301 aataggccca agcaactgtt tagcaaaaac acaggtctct gctaaagcgt aagctgatgt
      361 ataggggctg acgcctgccc ggtgctggaa ggttaagggg aacacttagc gcaagcgaag
      421 gtgtgaactt aagccccagt aaacggcggc cgtaactata acggtcctaa ggtagcgaaa
      481 ttccttgtca ggtaagttct gacccgcacg aatggcgtaa tgacttgggc actgtctcaa
      541 ctgtaaatcc ggcgaagttg tagtgcgagt gaagatgctc gctacccgcg attggacgga
      601 aagaccccgt agagctttac tgtagcttag cattgagttt tggtattgtc tgtacaggat
      661 aggtgggaga ctaggaaacc agggcgtcag ccttggtgga gtcaaccttg ggataccacc
      721 ctgacagtac tgaaattcta actggcggcc atgaatctgg tcacaggaca ttgttaggtg
      781 ggcagtttga ctggggcggt cgcctcctaa aaagtaacgg aggcgcccaa aggttccctc
      841 agaacggtcg gaaatcgttc gaagagtgca aaggcagaag ggagcctgac tgcgacaccc
      901 acaagtggag cagggacgaa agtcgggctt agtgatccgg tggtacctcg tgggagggcc
      961 atcgctcaac ggataaaagc tacctcgggg ataacaggct gatctccccc aagagtccac
     1021 atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc atcctggggc tgaagtaggt
     1081 cccaagggtt gggctgttcg cccattaaag cggcacgcga gctgggttca gaacgtcgtg
     1141 agacagttcg gtccctatcc gtcgcgggcg taggaaattt gagaggagct gtccttagta
     1201 cgagaggacc gggatggact gacctctggt gtaccagttg ttccgccagg agcacagctg
     1261 ggtagctaag tcgggaatgg ataaacgctg aaagcatcta agcgtgaagc caacct
//
LOCUS       DAEVGI010000294         1308 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEVGI010000294 DAEVGI010000000
VERSION     DAEVGI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.296"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            116..835
                     /locus_tag="K8M77_002571"
     CDS             116..835
                     /locus_tag="K8M77_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBI7086996.1"
                     /translation="MERLDKVLSNLGYGTRKEIKQVVKKGFVTVNDEKVKDSGLQIDP
                     EKDTIVINGEKVEYKKYIYLMMNKPAGVISATYDKRDETVIDLLYMEDQVFEPFPVGR
                     LDKDTVGLLLLTNDGDLNHKLISPKYHVDKVYYAEINAPITEKDIKAFENGVTLDDGY
                     KCMSAKLKVIENLEDSSRVEVTVQEGKYHQVKRMFEARGKKVIYLKRTSFGNLPLDES
                     LEEGEYKELSESELAVLYKNF"
BASE COUNT          524 a          114 c          227 g          443 t
ORIGIN      
        1 tctagttttt aaagatttaa ttatttaaga aggataataa gttgtaatta gttaaacttt
       61 gctttatact aaccacagat ataaatgtga ttttttacga aggagtgatt atttagtgga
      121 aagattggat aaggttttat caaacttagg atacggaacg agaaaagaaa taaagcaagt
      181 tgttaaaaaa ggatttgtaa cagttaatga tgagaaggtt aaagatagcg gacttcaaat
      241 agatcctgag aaggatacaa tagttataaa tggggaaaaa gtagagtata aaaagtacat
      301 atatttaatg atgaataagc cagctggagt aatttcagca acttatgata agagagatga
      361 aactgttatc gacttattat atatggaaga tcaagttttt gaaccatttc cagtaggaag
      421 acttgataaa gatacagtgg gattattatt attaactaat gatggagatt taaatcataa
      481 attaatttca cctaaatacc atgttgataa ggtttattat gcagaaataa atgctcctat
      541 aacagaaaaa gatattaaag ctttcgaaaa tggtgtaact ttagatgatg gatataagtg
      601 tatgagtgca aagcttaaag ttatagaaaa tttagaggat tcatctagag tagaagttac
      661 agttcaagag ggtaaatatc atcaagtaaa aagaatgttt gaagctagag ggaaaaaggt
      721 tatttattta aaaagaactt cttttggtaa tcttccactt gatgaaagtt tagaagaagg
      781 cgaatataaa gagctttcag aaagtgaatt agcggtgctt tacaaaaatt tttaaagatt
      841 taaatagtaa tgattgttgt gaaaaaatta atgtaggaaa acttgcaaaa aaaatattag
      901 actgcttgca atttaagtaa acatctaata taatagttgt gcgaggataa ataaaaggaa
      961 taaatagccc cctttttatt tattgcttat tgctgaaacg caacctagcc ccaagggttg
     1021 cgttttttat tttgtaaaag gatgcaagaa aaaaagcgtt tagaattagt tgttgaaata
     1081 atatattcat tttatataaa gagttggtac tttaaagtat caattctttt ttttatacta
     1141 tgatttaaat aaatattttt atattgagat aacatatata agcatttatt taaaaacttg
     1201 ttataaaaac tatttcatga aaactaaaag catactttgt aaaaagtaaa tttaatttta
     1261 ttcttaatat tatgtaatat tttttaaaat aaatattgtc tgagttat
//
LOCUS       DAEVGI010000295         1285 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEVGI010000295 DAEVGI010000000
VERSION     DAEVGI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.297"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(37..>1285)
                     /locus_tag="K8M77_002572"
     CDS             complement(37..>1285)
                     /locus_tag="K8M77_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7086997.1"
                     /translation="IISQFSTKKRKNKQEIYSFIVVIIFSSLLKVLNVYPNERIVICF
                     FIGLVYYKFNFKVNNIKSIMISLIFWLFMLTVEALSISFIVKINSLVSVSELLGKNIY
                     RVETMILSKVILISLIMVVRCLKLRVDIGKGDFIYILTPIATNIIIILVIFGYAFKSG
                     REGLGDNISIFFVSILLLLSNMSLMFIVSKIIKNNKLKLENEFIKDKLEMEYKYYSNL
                     EDNQEKVKRLYHDIKNHIACIEGNNKETDIRKKYIDSLNSEIDKLNLGFNTGNEVLDV
                     ILNNKREKCVENNISLKVFMDFSKVNFIEYFDICTIFSNCIDNAIEACKKIKDNNRYI
                     SLKGNCVNNFYVVKIENSKTNKIKKLNGDFLTDKKDKFLHGIGLKNIRLALEKYNGEI
                     IIEPLDDKFILKMLIPINQEKEA"
BASE COUNT          472 a          178 c           94 g          541 t
ORIGIN      
        1 tatataaata ataaaaaaga agcgtaggtt tttcatctag gcttcttttt cttgatttat
       61 aggaattaac atttttaata taaatttgtc atctaaaggt tctataatta tttctccatt
      121 atatttttct aaagctagtc ttatattttt aaggccaata ccatgtaaaa atttatcttt
      181 tttatcggtt aaaaaatctc catttaactt ttttatttta tttgttttag aattctctat
      241 tttaacaaca taaaaattat ttacacaatt accttttaaa cttatgtatc tgttattatc
      301 tttaatcttt ttacaagctt caattgcatt atcaatgcaa ttagagaaaa ttgtgcatat
      361 atcaaagtac tcaataaaat taacctttga aaaatccata aaaaccttta aagaaatatt
      421 attttctaca catttttctc tcttattatt taatattaca tctaacactt catttcctgt
      481 attaaatcct aagtttagct tgtctatttc tgaattaaga ctatctatat atttttttct
      541 tatatctgtt tctttattat ttccttcaat acaggctatg tgatttttaa tatcatgata
      601 aagtctcttt actttttctt gattatcttc taaattagaa taatatttat attccatttc
      661 taacttatcc tttataaatt cattttctaa ttttaatttg ttatttttta ttattttact
      721 aacaataaac attaaggaca tattagataa taacaataat attgaaacaa agaagatgga
      781 tatattatct cctaaacctt ctctaccact tttaaaagca tatccaaata taaccaagat
      841 aataattata ttagtagcta taggagtaag aatataaata aaatctccct ttccaatatc
      901 tacgcgtaac tttaaacatc taactaccat tattaatgat attaaaataa cctttgataa
      961 tatcatagtt tccactctat agatattttt acctaaaagc tccgatacac taactaaact
     1021 atttattttt actataaaag aaatagatag agcttctact gtaagcataa ataaccaaaa
     1081 tatcaatgaa atcataatag attttatatt gtttacttta aaattaaatt tataatatac
     1141 taaacctata aaaaaacata ttactattct ttcatttgga tatacattta gaacttttaa
     1201 caagctagaa aaaattatta ctacaatgaa agaataaatt tcttgtttat tttttctttt
     1261 cttagtgcta aattgactta taatt
//
LOCUS       DAEVGI010000296         1277 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEVGI010000296 DAEVGI010000000
VERSION     DAEVGI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.298"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            266..844
                     /locus_tag="K8M77_002573"
     CDS             266..844
                     /locus_tag="K8M77_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBI7086998.1"
                     /translation="MLMNTHILIAQNILRDVDVDFKISDKNFIYGNIKPDMVSKYKLK
                     KHYLNESFDMIVNMIKKLSSFNMYDFKKKFSVSRFSQELGVICHFICDFFCIPHSERW
                     EFKHSMNKHVKYEKELANFAKTYTPSQDYFKICGNISINVFLEECRKLYKKREGYEND
                     MNYAYFACRSIIKYISDSIVKNTKLIYSEAIA"
     gene            928..1238
                     /locus_tag="K8M77_002574"
                     /pseudo
     CDS             928..1238
                     /locus_tag="K8M77_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
BASE COUNT          559 a           94 c          186 g          438 t
ORIGIN      
        1 ttaaaataaa tcttatttta agaattatca ggtaaaaaaa gcaaaatgag aatataaata
       61 ataattatct ttaaaaggta ataattttaa gaattaaata ggtaaattgg gttaaaactg
      121 tagattagta agtatttcat acaataaata aaccgtttat taataattaa ttaataaatt
      181 aaaggtattt ttgtaatata cttttaattg atatgaaata tcatattaat aagaaacttt
      241 tttaagagat tagaggtaat aaattatgtt aatgaacact catattctta ttgcacaaaa
      301 tatattgaga gatgttgacg tagattttaa aataagtgat aaaaatttta tatatggaaa
      361 tataaaacct gatatggttt cgaaatataa attaaagaag cattatttaa atgaatcttt
      421 tgatatgata gttaatatga taaaaaaatt atcatcattt aatatgtatg attttaaaaa
      481 aaaattttca gtaagtagat tcagtcagga actaggagtt atatgtcatt ttatatgtga
      541 ttttttctgt attcctcata gtgaaagatg ggaatttaag catagtatga ataaacatgt
      601 taaatatgaa aaagagttag ccaattttgc taaaacatat actccatcac aggattattt
      661 taaaatatgc ggtaatattt caattaatgt tttcttagaa gaatgtcgta aattatacaa
      721 aaaaagagaa ggttatgaaa atgatatgaa ttatgcatat tttgcatgta gaagtattat
      781 taaatatata agtgactcaa tagttaaaaa tacaaaatta atatattctg aagctatagc
      841 ttaatacaaa tattaccttt ttatgataaa atatgttaaa aaaattatat agcaatttaa
      901 gtgattttta tatagaaagg aaataagatg gagagtaagg taaatgaacc tatatataag
      961 caaattgctt tagatattgc aggtaggata tataataatg acataaaggt aggacaaaag
     1021 atacatggta gattaacttt agcaagtagt tacaatgtat catcagaaac agttagaaaa
     1081 gcttttaaat tattagaaga catgaaagta gtatcgtcaa gcaaggagag cttataatat
     1141 ctccaggacc atattttaaa tttgaaaaag atgataatct ccttgtagtt ggagaagaag
     1201 acatattaaa aaagatagaa atgtttttaa ataaataatg gaaaaagggc tgtattaaaa
     1261 tataataata aaactta
//
LOCUS       DAEVGI010000297         1254 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEVGI010000297 DAEVGI010000000
VERSION     DAEVGI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.299"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..307
                     /locus_tag="K8M77_002575"
     CDS             <1..307
                     /locus_tag="K8M77_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7086999.1"
                     /translation="KYNITLVIICEKQKIENQQILESNKILKEEKSTNICRKIFIFNE
                     KDSLLSELNYLPFYFDDIKHTDSDRVIALEDELDMIVKEATIFLENLDKSQNEENKC"
     gene            301..>1254
                     /locus_tag="K8M77_002576"
     CDS             301..>1254
                     /locus_tag="K8M77_002576"
                     /inference="COORDINATES: protein motif:HMM:NF024867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI7087000.1"
                     /translation="MLIIKKIEIKNFKIYKEENIDFGENKFIMLTGPNGYGKTSLIDA
                     IEWCITGTIKRLELKHSNRCKKAKEKKRVENSVGILKNKNCDRDEFVSVKLTFICDGE
                     EVTILRERKKDSLEIDEEFEIVGRISNENIKEKIEKIKTENSFYKYNFLDMSKSYRFM
                     ETSRVDLKEQVSDFIKDRNNINHLIDNLNEKISEIDIKINNNKENIVKNETEKDKIQK
                     EIENLAEFSDIKEYPNIASYIGDPLNLKSIEEAKNVDKLIRGWGYNYVYENILNLNKS
                     KNAEKNKKELEEIKNEFIKEENLIIKFIEEKFYEKEKRNKIS"
BASE COUNT          621 a           72 c          195 g          366 t
ORIGIN      
        1 aaaatataat attactcttg ttattatttg tgaaaaacaa aaaatagaaa atcaacaaat
       61 attagaaagt aataaaatat taaaagaaga aaaatcaact aacatatgta gaaaaatatt
      121 tatttttaat gaaaaagata gcttactaag tgaattgaat tatttaccgt tttattttga
      181 tgatattaaa cacactgatt cagatagagt tatagcgtta gaagatgaat tagatatgat
      241 agttaaagaa gctacaattt ttttagaaaa cttagataaa agtcagaatg aggaaaataa
      301 atgttaataa ttaagaaaat agaaataaag aattttaaaa tatacaaaga agagaatata
      361 gattttgggg aaaataaatt tataatgctt actggaccta atggttatgg gaaaacatct
      421 ttaatagatg ctatagaatg gtgtattaca ggaactataa agaggttaga attaaaacat
      481 tcaaatagat gtaaaaaagc taaagaaaaa aaacgtgtag agaatagcgt aggaatatta
      541 aaaaataaga attgtgatag agatgaattt gtcagtgtca agttgacatt tatatgtgac
      601 ggagaagaag taactatttt acgtgaaagg aaaaaagatt ctttagaaat tgatgaagaa
      661 tttgaaatag taggaagaat ttctaatgaa aatattaaag aaaaaataga aaaaattaaa
      721 acagaaaatt cattttataa atataatttt ctagatatga gcaagtcata tcgttttatg
      781 gaaacaagta gagtagattt aaaggagcaa gtgagtgatt ttataaaaga cagaaataat
      841 ataaatcatt taattgataa tttaaatgaa aaaataagtg aaatagatat aaaaataaat
      901 aataataaag agaatatagt taaaaatgaa acagaaaaag ataaaattca aaaagaaata
      961 gaaaatttgg ctgaattttc agatataaaa gaatacccta atatagcttc ttatataggt
     1021 gaccctttaa atttaaaatc catagaagag gcaaaaaatg ttgacaagtt aataagagga
     1081 tggggatata attatgtata tgagaatata ttaaatttaa ataaatcaaa aaatgcagaa
     1141 aaaaataaaa aagagttaga agaaataaaa aatgaattta ttaaagaaga aaatttaatt
     1201 attaaattta ttgaagaaaa gttttatgaa aaagaaaaaa gaaataaaat tagt
//
LOCUS       DAEVGI010000298         1252 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEVGI010000298 DAEVGI010000000
VERSION     DAEVGI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.300"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            61..831
                     /locus_tag="K8M77_002577"
     CDS             61..831
                     /locus_tag="K8M77_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBI7087001.1"
                     /translation="MDFNRIINFSTELGTLMLENGAETYRVEETMSRICLVYGIEKVD
                     VFVIPTNIIITIKIYKNAISRTRRVTSRTINLDKIAKLNNLSREVAFNKVSIEDAEKK
                     LSIIADEKEYPLKIKILGYLISASTFTLLFGGNSKDSLVSGFIGIVLCLLAYFFNILK
                     TNNFFVNIISGALASLLAFLTVKFNIAPNLNEIIIGSLMPLVPGLAITNSLRDIIAGD
                     LVSGSAKFIEAFLIAVGIAIGSASILSILINHYGGMAI"
     gene            828..>1252
                     /locus_tag="K8M77_002578"
     CDS             828..>1252
                     /locus_tag="K8M77_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBI7087002.1"
                     /translation="MNLIINSIYAFFSSLGFGIIFNIKGKNLFFSSLCGGLGWFVYLF
                     SSSFISTNESFNYFLAAIFIALFSEISARVLKSPVLVFLVCGIIPLVPGGGMFYTMLE
                     TLKGNIDKALSLGCNTLMLAGSIATGIVLVSSFTKLYFS"
BASE COUNT          417 a          160 c          193 g          482 t
ORIGIN      
        1 atattttaat aatgaaaaaa tatttcttat taatttttac atacttattt ggaggtaaat
       61 atggatttta atagaattat caatttctca actgaacttg ggactctaat gcttgaaaat
      121 ggtgctgaaa catatagagt tgaagaaacc atgagcagaa tctgtttggt ttatggaata
      181 gaaaaagttg atgtctttgt aattcctaca aacataataa taacaataaa aatatataaa
      241 aatgcaattt ctagaactag aagggtaaca agtagaacta ttaatttaga taaaatagct
      301 aaattaaaca acctttcaag agaggttgcc tttaataaag tttctattga agatgctgaa
      361 aaaaaactca gtattatagc tgatgaaaaa gaatatcctc ttaaaataaa aatattagga
      421 tatttaattt cagcttcaac ctttactcta ctttttggag gtaattcaaa ggattcttta
      481 gtctcaggtt ttataggaat agttctttgt cttcttgcat acttttttaa tattttaaag
      541 acaaacaatt tctttgtaaa tataatatct ggagctttgg catcattact agcttttctt
      601 actgtaaaat ttaatattgc tcctaatcta aatgaaataa taataggttc tttaatgccc
      661 ttagtacctg gacttgctat aactaactca cttcgtgata taatagctgg tgatttagtt
      721 tctggaagtg ctaaatttat tgaagccttt ttaatagctg tagggattgc tattggaagt
      781 gctagtattt tatctatatt aataaatcac tatgggggta tggctatatg aacctaataa
      841 taaactcaat atatgcattt tttagttctt taggttttgg aataatattt aatataaaag
      901 ggaaaaactt atttttctct tctttatgtg gcggtttagg atggtttgtt tatctttttt
      961 cctcaagttt tattagcaca aatgaaagct tcaattattt tttagctgct attttcatag
     1021 ccctattctc tgaaatttca gctagggtat taaagtctcc tgtattagta tttttagttt
     1081 gtggaataat tcctctagtt cctggaggag gcatgttcta tacaatgcta gaaaccttaa
     1141 aaggaaatat agacaaggct ttatccttag gatgcaatac actaatgtta gctggttcta
     1201 tagctactgg aattgtttta gtttcatcct ttactaagct atatttttct at
//
LOCUS       DAEVGI010000299         1250 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEVGI010000299 DAEVGI010000000
VERSION     DAEVGI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.301"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            106..714
                     /locus_tag="K8M77_002579"
     CDS             106..714
                     /locus_tag="K8M77_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087003.1"
                     /translation="MNKFTSFLKKRKAFVIVALIGMIFGFTFFSAPQKDINKLKAEKT
                     TVLAKIDSSQEELSTLNKDIGDLKEKINQLNDEKQKQEQLLKDTTAKVELKQKEEAEK
                     QRQAEIEKQKQEEANSISQSSSNNSSKKSTSNNSSNAKSSSYNSDNPQPSKPVGEMVY
                     ITATGKKYHRKNKCGNTNPARTSYIPLSEAESMGYSPCSKCY"
     gene            743..1033
                     /locus_tag="K8M77_002580"
     CDS             743..1033
                     /locus_tag="K8M77_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087004.1"
                     /translation="MKNTLKKILKFLLGICIFFIAICIGSSLKDDSNLNNTHNELVTT
                     INNSKEDLTHKEEELSKAKTEKEDLEKQLSTLNDSLNKTKESIKQLDHSTNN"
BASE COUNT          534 a          152 c          152 g          412 t
ORIGIN      
        1 taattaatat tatttactta aaatttaatt tttagcaaca ttttatacat tttattctaa
       61 atttatgtta tattagttca tggacataaa tgtaaggagg acaaaatgaa taaatttaca
      121 tcatttttaa agaaaagaaa agcttttgtt atagtagctt taattggtat gatttttggt
      181 tttacttttt ttagtgcgcc tcaaaaagat attaataaat taaaggcgga aaaaactact
      241 gtattagcaa aaatagatag ctcgcaagaa gaactatcta ccttgaataa agatataggt
      301 gatttgaagg aaaaaataaa tcaacttaat gatgaaaaac aaaaacagga acaactttta
      361 aaagatacca cagccaaagt agaactaaaa caaaaagaag aagctgaaaa acaaagacaa
      421 gctgagattg aaaaacaaaa acaagaagaa gcaaatagta ttagtcaaag ttcttcaaat
      481 aattctagta aaaagtctac tagtaataac tcatctaacg ctaaatcaag ctcatataat
      541 tctgataatc cacagccttc taaaccagtt ggtgagatgg tgtacataac tgcaacaggt
      601 aaaaaatatc atcgtaaaaa taaatgtggt aatacaaacc cagctaggac aagttatatt
      661 ccactatcag aagcagaatc tatgggatac tccccttgtt caaaatgtta ttaataaaat
      721 ttagaaagga ctttactata ttatgaaaaa tacactaaaa aaaatattaa aattcttatt
      781 agggatatgt atatttttta tagctatttg tattggttcc tcactaaaag atgattcaaa
      841 tttaaataat actcataatg aattagttac cacaattaac aattcaaaag aggatttaac
      901 tcataaagaa gaggaattgt ctaaggctaa aactgaaaaa gaggatttag aaaaacaatt
      961 aagtactcta aatgattcat taaataaaac taaggaatct attaaacaat tagatcatag
     1021 cactaataat taatttttat aatattgatt tttttaacta tacacatgtc acaagaacca
     1081 gtttttaact tttaataact ggttctttat ttttttcttt gattaatttt ctaattgttt
     1141 aaaatcccta aaacacttct aatttgtaaa aaagttttat attaaattcc ttataatata
     1201 aactctttta tcttaaataa tgtataattt atataagaag taattaacat
//
LOCUS       DAEVGI010000300         1248 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEVGI010000300 DAEVGI010000000
VERSION     DAEVGI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.302"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            115..855
                     /locus_tag="K8M77_002581"
     CDS             115..855
                     /locus_tag="K8M77_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087005.1"
                     /translation="MKKTLYDYEESIKKLPLKEKYTKEELLIDDFLVDKEGNIEIYYA
                     PHNEYINPKAKVFIIGITPGFNQMSTAIATARKELEIGDNIKEIQYKCKVAGRFSGIL
                     RKNIISMLNEIELNEALKINDCGELFGEKDYLLHTVSLIPYSVFLKKANYTGHTPKLI
                     KSDFLMKYVYENFIEEIRSLDDFENILLIPLGKAVEEVLFKLKEEGFISDGQILKGFP
                     HPSGANVNRIPQLKENKESMIKKIKEFY"
BASE COUNT          502 a           97 c          214 g          435 t
ORIGIN      
        1 acacaaaagt tacaaaaaat gaatttttat ataaaaataa acaataatat atgttgaatt
       61 aagattatat tattactata tttattttat agaatattat ttggagatgt taatatgaaa
      121 aagactttat acgattatga agaatcaatt aaaaaactac ctttaaaaga aaaatataca
      181 aaagaagaac ttttaataga tgattttctt gttgataaag aggggaatat tgaaatttat
      241 tatgctccac ataatgaata tataaatcct aaggctaaag tgtttataat tggtataact
      301 ccaggtttta atcaaatgag tacagctata gctacagcaa gaaaggaatt agagattgga
      361 gataatatta aagaaattca atataaatgc aaggtggcag ggcgttttag tggaatctta
      421 agaaaaaata taattagtat gttaaatgag atagagctta atgaggcttt gaaaataaat
      481 gattgtggag agctttttgg agaaaaggat tatttattac atacggtatc tttaattcct
      541 tactctgttt ttcttaagaa agcaaattat acaggccata ctccaaaact tataaagagt
      601 gattttttaa tgaagtatgt ttatgagaac tttattgagg aaattaggag tttagatgat
      661 tttgaaaata tattattgat acctttagga aaagcagttg aggaagtttt atttaagctt
      721 aaggaagaag ggtttataag cgatggacaa atacttaagg gatttccaca tccatcaggt
      781 gcaaatgtaa atagaatacc tcaattaaag gaaaataaag aaagtatgat taaaaaaatt
      841 aaagagtttt attaacttac attaattaaa ttcccagtgg cttattatgg agatttcact
      901 tttatattta attttgaaaa tttatttata atagtcttta atataaaaag ctttaattta
      961 aattttcaat gggataatat ggtgtgataa aataattaag tgaattaaat tattttatct
     1021 aagagggaag tatataatga agaatattaa aatgagatat ccaatatatc ttaaagagtt
     1081 taagtgtatt ggggggatgt gactgttgtt ataaaaaggt aataatattt ataaaatctt
     1141 aggatggtta aaatgtttgt gtttaggata aaatattgat agctattatg aatcccatat
     1201 attgctaaag tgatatagtc atagggtaga accttgaggg ttttagtg
//
LOCUS       DAEVGI010000301         1240 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEVGI010000301 DAEVGI010000000
VERSION     DAEVGI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.303"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..873
                     /locus_tag="K8M77_002582"
     CDS             <1..873
                     /locus_tag="K8M77_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBI7087006.1"
                     /translation="IKVVTMIGCNLANKASINKEQKEQEKVIKPNPDDVMPRMNITYE
                     ADKYSVDAKDVQAMANDTYKGEGKYVFLTFDDGPSNSTEKILNILKDKNVHGTFFVLG
                     SSIEKDSKRQEYLKEELKSGNAIANHSYSHDFKKLYPGNKLKIDSFMDEFNKTNDIMK
                     SILGGEFDCKVLRMPGGYGTRKYYKDPSLKEFDDTLQENGIINVDWNALDGDAEGKPY
                     STEEMLNYVKKTSKGKNHVVILMHDAAGKEKTVEILPQIIDYYKNEGYEFKVIKNTSK
                     EENNSNQSENNKDK"
     gene            complement(921..1220)
                     /locus_tag="K8M77_002583"
     CDS             complement(921..1220)
                     /locus_tag="K8M77_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087007.1"
                     /translation="MNIDNIKEVQAKSNKMISEIHFKEQCSWLRNSKKKKDLKNNFSK
                     EIYEDLKDKKIYLELLEKEISDIFSSKDFTSEEKNHIYELTNESFWKWKLNIDKK"
BASE COUNT          509 a          137 c          192 g          402 t
ORIGIN      
        1 ataaaagttg tcacaatgat aggctgcaat ttagctaata aggcttcaat taataaagaa
       61 cagaaagaac aggaaaaggt cataaagcct aatccagatg atgtaatgcc tagaatgaat
      121 attacatatg aagcagataa atattcagtt gatgctaaag atgtacaagc aatggctaat
      181 gatacatata aaggtgaagg aaaatatgta tttttaacct ttgatgatgg accatctaat
      241 tcaacagaaa agatattaaa tatattaaaa gataagaatg tacatggaac attctttgtt
      301 ttaggaagta gtatagaaaa agactctaaa agacaagagt atttaaaaga agaattaaaa
      361 agtggaaatg ctattgcaaa tcatagttat agtcatgatt ttaaaaaatt atatccagga
      421 aataagctaa aaattgattc ctttatggat gagtttaata agactaatga tataatgaag
      481 agtattttag gaggagaatt tgattgcaag gtattaagaa tgccaggtgg gtatggaaca
      541 agaaaatatt ataaagatcc ttctcttaaa gaatttgacg atacattaca ggaaaatgga
      601 ataataaatg ttgattggaa tgccttagat ggtgatgcag agggaaaacc atattctaca
      661 gaagaaatgc ttaattatgt gaaaaaaaca tcaaaaggaa aaaatcatgt agtcattctt
      721 atgcatgatg cagcaggaaa agaaaaaacc gtagaaatat taccacaaat aatagattac
      781 tacaagaatg aaggatatga gtttaaagtt ataaaaaaca cttctaagga agaaaacaat
      841 agcaatcaaa gtgaaaataa taaagataaa tagagtaaat agtataagaa aacatcatta
      901 gaaaatacta atgatgtttt ttatttttta tctatattaa gtttccattt ccaaaaggat
      961 tcattagtta attcatatat gtgattcttt tcttcacttg taaaatcttt gctagaaaaa
     1021 atatcactaa tctccttttc aagtaactct aaatatattt ttttatcttt taggtcctca
     1081 taaatttctt ttgaaaagtt attttttaaa tcttttttct tttttgagtt tctaagccag
     1141 ctacattgtt ctttaaaatg aatttcagag atcattttat tactttttgc ttgaacctct
     1201 tttatattat caatattcat aaaaactcct ttctgaaaga
//
LOCUS       DAEVGI010000302         1225 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEVGI010000302 DAEVGI010000000
VERSION     DAEVGI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.304"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(84..731)
                     /gene="fsa"
                     /locus_tag="K8M77_002584"
     CDS             complement(84..731)
                     /gene="fsa"
                     /locus_tag="K8M77_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI7087008.1"
                     /translation="MKIFIDTANVEEIRKASKLGVLAGVTTNPSLIAKEGRDIKEVIE
                     EICSIVDGPISAEVMALECDEMVREGRELAKIHKNIVIKIPMCEEGLKAVKVLASEGI
                     RTNVTLIFSPLQALLAARAGASFVSPFLGRLDDIGNPGIEIVTQIAEMFALHGIDTEI
                     ISASVRNPMHVLDSAMAGSHIATIPYNVILQMVKHPLTDAGMKKFIEDYNKAFNK"
     gene            complement(1128..>1225)
                     /locus_tag="K8M77_002585"
     CDS             complement(1128..>1225)
                     /locus_tag="K8M77_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBI7087009.1"
                     /translation="LDAIHDTVHEMCKDEARHGKAFEGLLKRYFE"
BASE COUNT          394 a          191 c          125 g          515 t
ORIGIN      
        1 aggattaatt gttgactata agtaaaatat attttttata aatataattt aatgtattaa
       61 ttcatattgt aaatacaatt atattattta ttgaaagctt tgttataatc ttcaatgaat
      121 tttttcatac cagcatcagt taaaggatgt tttaccattt gaagtataac attataagga
      181 attgttgcaa tgtgtgatcc tgccatagct gaatctaaaa catgcattgg atttctaaca
      241 cttgcagata ttatttcagt gtctattcca tgtaaagcaa acatttcagc gatttgagtt
      301 acaatttcaa ttcctggatt tcctatatca tcaagtcttc ctaagaatgg acttacgaat
      361 gatgcaccag ctctagctgc taataaagct tgtaatggtg agaagattaa agtaacattt
      421 gttcttattc cttcactagc taatactttt acagctttta aaccttcttc acacataggt
      481 atttttataa ctatgttttt atgtatttta gctaattctc ttccttctct aaccatttcg
      541 tcgcactcta aagccataac ttcagcactt attggtccat ctactatgct acatatttct
      601 tcaattactt ctttaatatc tctgccttct tttgctatta atgaaggatt agttgttact
      661 ccagctaaaa ctcctaattt actagccttt cttatttctt ctacattggc agtatcgata
      721 aatattttca tttcataatc tccttttcat tactttaaaa atattaattt gaattccata
      781 cttttctatt aatcgtttcc acagatagat attaacacta caaaataaat tgttcaatat
      841 ttttatttaa agtttacact ttgttaattt atttttccta tatattatgc tataatatat
      901 tattttagta gtttaatata tcatattaag ctattagagt acctcttacg taatctttct
      961 catttaaagt gtacacaaat aatataatta atcttccact atttattctg tgtgaaattt
     1021 ataattatat ttcaatataa aaaacttaat tttcaatata aatactttat attaaaaatt
     1081 aaaaaaagat gatttctata tttgtagaaa tcatcttttt attttaatta ttcaaaatat
     1141 ctttttaata atccttcgaa agcttttccg tgtctagctt catctttaca catttcatga
     1201 actgtgtcgt ggattgcatc taagt
//
LOCUS       DAEVGI010000303         1223 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEVGI010000303 DAEVGI010000000
VERSION     DAEVGI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.305"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..770
                     /locus_tag="K8M77_002586"
     CDS             <1..770
                     /locus_tag="K8M77_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBI7087010.1"
                     /translation="ITEFKFSEKIKFDLGKRKLSEKIKNDFLSSISHDLKTPINVIYS
                     SVQVQEKFYVDENINKIGHYNQVNKENCITLMRLANNLIDSSKIDYDYLKPNFKVYNI
                     VTLVEDSILNLAEYIKEKKLTYIFDTDEEELYVKCDQEFIQRIILNLISNSIKYTKRG
                     GIRVQIKSNRNKVIIDFTDTGEGMDKDFIEKAFLRYSKGNKKGIKNKSTGIGLYIVKS
                     LVELQNGTIYINSIKDAGTNVRLEFSREKNCGIQSGI"
     gene            748..>1223
                     /locus_tag="K8M77_002587"
     CDS             748..>1223
                     /locus_tag="K8M77_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7087011.1"
                     /translation="MEYKVEYENPRLQYYILRIIYISIILSSIIFIKKEDYIIFYKFL
                     DLCSVIMLGTSLLIGISSNIDKEKNLISYTNIGIFWLLIPEIFYSFTSLELYVVDKMI
                     VYKIIGLLFQYFIVFVIALGKKKNFNFKKINKIFLILNITFNITYLLVINNVKLMKV"
BASE COUNT          541 a           85 c          166 g          431 t
ORIGIN      
        1 atataacaga atttaaattt tcagaaaaga taaaatttga tttaggaaaa agaaaattat
       61 cagaaaaaat aaaaaatgat tttttatcaa gcatatctca tgacttaaag acacctataa
      121 atgtcatata ttcatcagta caagttcagg agaagtttta tgttgatgaa aacataaata
      181 aaataggtca ttataatcaa gttaataagg aaaattgtat tacattaatg agattagcta
      241 ataatctgat agattcatca aaaattgatt atgattatct aaagccaaat tttaaagttt
      301 ataacatagt aactttagta gaagattcta tattaaattt agctgagtat ataaaagaaa
      361 aaaaattgac ttatatattt gatacagatg aagaggagtt atatgttaaa tgcgatcaag
      421 aattcattca aagaattatt ttaaatttaa tttctaattc aataaaatat accaaaagag
      481 gtggaataag agtacaaata aaatcgaata gaaacaaggt tattattgat tttacagata
      541 ctggagaagg tatggataaa gattttatag aaaaagcttt tttaagatat agtaaaggaa
      601 ataaaaaagg aataaaaaat aaaagtacag gaattggatt atatattgtt aagagtttag
      661 ttgaactaca aaatggaact atatatataa attctattaa ggatgcagga actaatgtta
      721 gattagagtt tagtagggag aaaaattgtg gaatacaaag tggaatatga aaatcctaga
      781 ttgcaatatt atatattaag aataatatac atatcaataa tattatcatc tataatattt
      841 ataaaaaaag aagattatat tatattttat aaatttttag atttatgctc agtaataatg
      901 ttaggaacat cattactaat aggtataagt agtaatatag ataaagaaaa gaatttaatt
      961 tcctatacta atatagggat attttggctt cttattcctg aaatttttta ctcatttact
     1021 tcattagaac tatatgtagt agataagatg atagtttata aaataatagg gttattattt
     1081 caatacttta ttgtatttgt tatagcatta ggaaaaaaaa agaattttaa ttttaaaaag
     1141 ataaataaga tttttttaat attaaatatt acatttaata ttacttatct attagttatt
     1201 aataatgtta aattaatgaa agt
//
LOCUS       DAEVGI010000304         1221 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEVGI010000304 DAEVGI010000000
VERSION     DAEVGI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.306"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(220..1143)
                     /gene="glsA"
                     /locus_tag="K8M77_002588"
     CDS             complement(220..1143)
                     /gene="glsA"
                     /locus_tag="K8M77_002588"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBI7087012.1"
                     /translation="MDINLDALVEANRKYTKDGHVATYIPALAKVNPNQLGVCIYDLK
                     ENKKFSAGEYDVRFAIESISKVPTLILAILDNGIEKVFSEVGTEPSGFAFNSIMNMQI
                     NHKNKPSNPFINAGAIKVVSLLKGKNDEERFKRILDFYRKIMNDDEITLDTEIYLSER
                     ETGDINRSLAYYMKGNGIMEGDVTDILDSYFKQCSVLVTAEDLARLGAVLANEGVMPW
                     NGERLFSVETAIVVKSLMTTYGLYDESGAFSVHIGLPSKSGVGGGILSSVPNKCGIGL
                     FSPALDVPGNSVASMKLLKEIADKLKLDIFR"
BASE COUNT          442 a          207 c          106 g          466 t
ORIGIN      
        1 tagaatattt ttattttaga attaatttaa acaaaatatt tgtataaagt atgttattaa
       61 atataaaata aatattaacc tacttattaa tctctacact attaaatatt ctttaaccta
      121 taaaatagtt gttaaattct aaaaaatata ggcttaaata tatttagatt tttaatcact
      181 ctaaacatac ttaaacctaa tttacttatt taaaaacttc tatctaaata tatctaattt
      241 taatttatct gctatttcct ttagaagttt catactagca acactatttc ctggtacatc
      301 taaggctgga ctaaataatc ctataccaca tttattagga actgaagata gtattcctcc
      361 tcctacacca cttttagatg gtagtccaat atgaactgaa aaagctcctg attcatcata
      421 taatccataa gtagtcatta atgatttaac aacaatagct gtttctacag aaaataatct
      481 ttcaccattc caaggcataa ccccttcatt agctaataca gcacctaatc ttgctaaatc
      541 ctcagctgta actaaaaccg aacattgttt aaaatatgaa tccaaaatat cagtaacatc
      601 accttccatg attccattac ccttcatata ataagcaaga gatctgttta tatcacctgt
      661 ttctctttct gataaatata tttcagtatc taaagtaatt tcatcatcat tcataatctt
      721 tctataaaaa tctaaaattc ttttaaaacg ttcttcatca ttttttccct taagtaagga
      781 tactacttta atagctcctg cattaataaa tgggtttgat ggtttatttt tatgatttat
      841 ttgcatattc ataattgaat taaaagcaaa tccagagggt tctgttccaa cttctgaaaa
      901 tactttttct attccattat ctaatatagc taatattaat gtaggaacct ttgatatgct
      961 ttcaattgca aatctaacat catattctcc agctgaaaac tttttatttt cttttaaatc
     1021 ataaatacaa acacctaatt gatttggatt tacctttgca agggctggaa tatatgtagc
     1081 tacatgtcca tcttttgtat attttctatt tgcttctact aaagcatcta aatttatatc
     1141 cataaaaatc acctctaaaa ttatcttata aaaatattat aaacagttaa taatttcaaa
     1201 atcaaaattc aaaaatttta c
//
LOCUS       DAEVGI010000305         1209 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEVGI010000305 DAEVGI010000000
VERSION     DAEVGI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.307"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(95..667)
                     /locus_tag="K8M77_002589"
     CDS             complement(95..667)
                     /locus_tag="K8M77_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBI7087013.1"
                     /translation="MILIRLFLEFFKVGLFAVGGGAATIPFLYNISDKTHWFTHTDLA
                     NMIAISQSVPGAMGINMATYVGYATYGVLGAITSTIGIIVPSIIIILIVARVLAKFKE
                     NKRVADCFYGLRPASTGLLLAAGFEIVKISILTLNKYEETHNITDILSIKALILAGIL
                     FFFIRKYKKSPIFYIIASAIVGIIFNFAKE"
     gene            complement(654..1205)
                     /locus_tag="K8M77_002590"
     CDS             complement(654..1205)
                     /locus_tag="K8M77_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBI7087014.1"
                     /translation="MKELINLYFLFIKLGFMNFGGGYALFPILQKEIIEKRNWITNEE
                     LMDYYAIGQCTPGVIAVNTATFIGYKRKGNIGGILATLGFVTPSLIMITIIATFIGNF
                     SNLPIVKNAFAGIRVCVAVLILNTAINLIKTSVIDIKTCIILVLVAGFSIFTDISPVL
                     YVIIAGIAGIVLRSIGGKKNDTN"
BASE COUNT          437 a          189 c          126 g          457 t
ORIGIN      
        1 ttaaagaatc ctattcttaa aataaatttc tttaatactt ttatctttta aaataataaa
       61 tgccttttac tactaagtta ttatttattt ttctttattc tttagcaaaa ttaaatatta
      121 tacctactat agctgaggca ataatataaa aaattggtga ctttttatat tttcttataa
      181 aaaagaataa aattcctgct aatattaaag ctttaattga taatatatct gtaatattat
      241 gtgtttcttc atatttattt agagttaata ttgatatttt tactatttca aagcctgcag
      301 caagtaaaag tcctgttgat gctggtctta atccataaaa acaatcagcc actcttttat
      361 tttctttaaa ttttgctaag actcttgcta caattaaaat tataattatt gaaggtacta
      421 taatacctat ggttgaggta atagcaccta aaactccata ggttgcataa ccaacataag
      481 ttgccatatt gattcccata gcacctggta ctgattgaga tattgcaatc atgtttgcaa
      541 gatcagtatg tgtaaaccaa tgtgttttat ctgaaatatt atatagaaat ggtatagtag
      601 cagctccacc acctacagca aaaagaccaa ctttaaaaaa ttctaaaaat aatctaatta
      661 gtatcattct ttttacctcc aattgacctt aatactattc ctgctattcc tgctattatt
      721 acatataaaa caggagatat atctgtaaat atagaaaatc cagctactaa tacaagaata
      781 atacatgttt taatatcaat taccgatgtc ttaattaaat ttattgctgt atttaaaatc
      841 aatactgcta cacatacacg aatcccagca aaggcatttt taactattgg aagatttgaa
      901 aaatttccaa taaatgtagc aattattgtt atcattatta atgaaggagt aacaaaacca
      961 agagtcgcca gtatccctcc tatatttccc tttcttttat aacctataaa tgtagctgta
     1021 tttactgcaa ttactcctgg tgtacattga ccaattgcat agtaatccat taattcttca
     1081 ttagtaatcc aatttctttt ttcaattatt tctttttgta atattggaaa taaagcatat
     1141 cctcctccaa aattcataaa tcctaatttt ataaaaagaa aatataaatt aattaattct
     1201 ttcatatat
//
LOCUS       DAEVGI010000306         1208 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEVGI010000306 DAEVGI010000000
VERSION     DAEVGI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.308"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            103..>1208
                     /locus_tag="K8M77_002591"
     CDS             103..>1208
                     /locus_tag="K8M77_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7087015.1"
                     /translation="MNRNRLSCFIVGAVIGAGAIVCTTNTKVHAKPVNEVKNINTSKG
                     NLFGEIISSEDLGLRKGADSSHEIITSIPSGARVNIIDKVSDNWYKVGYKDFVGYVEA
                     KDIRILGYNLNQDNVALISANQLNVRTSPNENGQVIGTLYKNNKVNVLDKSIDGWYKI
                     DFNGRRAYVSSKYVNLISYKNNEVKTEVKKDPIEGTGKVNINTALNVRQASTTSSRII
                     GSLKGGEKVNIINESNGFYKIEFNNSYGYVYSKYISKDGGDEKAQIVKQEEVKKEKVD
                     ESKKEAKSTTKAEPIVLAIRYLNKTGIVNVSSSLNVREGASTSSKVIGSLSGNSKVTI
                     VGEEGAFYKIEYKGSHGYVAKEYVKDVTENSNSN"
BASE COUNT          514 a          108 c          226 g          360 t
ORIGIN      
        1 aattataaaa aattaattat tttccttaga attaacattg atttataatg aattttttat
       61 gattttatat aaaatactaa ttttttataa aaggagggta aaatgaatag aaatagatta
      121 agctgtttta tagtaggtgc tgtaattgga gcaggagcaa tagtttgtac aactaatact
      181 aaagtacatg cgaaaccagt gaatgaggtt aaaaacatta atacttctaa aggaaatttg
      241 tttggagaaa ttatttcatc agaagattta ggattaagaa aaggtgcaga ttcttctcat
      301 gaaataataa cttcaatacc tagtggcgcg agagttaaca taatagacaa ggtatctgat
      361 aactggtata aagttggtta taaagatttt gtagggtatg tagaagctaa ggatataaga
      421 atactaggat ataatttaaa tcaagataat gttgctttaa tttctgctaa tcaattaaat
      481 gttagaacta gcccaaatga aaatgggcaa gtgatcggaa ctttatataa aaataataag
      541 gtaaatgtat tagataaatc aattgatggt tggtataaaa ttgactttaa tggtagaaga
      601 gcatatgtat ctagtaaata tgttaatttg atttcatata aaaataatga agttaagaca
      661 gaagtgaaaa aagacccaat tgaaggaaca ggtaaggtta atataaatac agctttaaat
      721 gttagacaag cttctactac aagtagtaga attattggta gcttaaaagg tggggaaaaa
      781 gttaacataa taaatgaaag taatggattt tataaaatag agtttaataa ttcatatggc
      841 tatgtttatt ctaaatacat atcaaaagat ggaggcgatg aaaaggctca aattgtaaaa
      901 caagaagaag ttaaaaaaga gaaagttgat gaatctaaaa aagaagctaa atctactact
      961 aaagcagaac ctatagtttt agctattaga tatcttaata agacaggaat agtaaatgta
     1021 agtagctccc taaacgtaag agaaggagca agtacaagta gtaaagttat aggaagccta
     1081 agcggaaact caaaagtaac aatagtagga gaagaaggag ctttctataa aatagaatat
     1141 aaaggttctc atggatatgt agctaaggaa tatgttaaag atgttacaga aaatagtaat
     1201 agtaacca
//
LOCUS       DAEVGI010000307         1177 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEVGI010000307 DAEVGI010000000
VERSION     DAEVGI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.309"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            67..1002
                     /locus_tag="K8M77_002592"
     CDS             67..1002
                     /locus_tag="K8M77_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBI7087016.1"
                     /translation="MEIKVNNRVKLFLKITFISVVLVLVAFGFKNMFKEFNMTYFNMY
                     RDKLHFFKLTLIAISGIIAYMPLSTYDFILKKDVGIGINNLKLYKYSWIASSIASLLG
                     FGGATSLAFKQYFYGNHVEDKKKLLKEIGKIIFLNLTGLSILCLIYLLLEFKDIQNLG
                     IIKYVVYGVSAYAPLIIINSLYRYKIKKEKDEIILKVVCISFIEWTLNMLLIYLILII
                     TGAHIKLAKYFCVYVRATAVAIVSMVPGGIGTFDLTFIDGFKALGVPIEQTFLVIILY
                     RISYFILPAGIGVILYIHEFGMNFKALIKIRNNKN"
BASE COUNT          462 a           95 c          179 g          441 t
ORIGIN      
        1 attttattta aattaatgta aaataatatt aatgtataat taataaatgt aaaggagagg
       61 aaattattgg agattaaagt gaataataga gttaaacttt ttttaaagat aacttttata
      121 tcagtggtat tagttttagt ggcctttgga tttaaaaata tgtttaaaga atttaatatg
      181 acttatttta atatgtatag agataaattg cattttttta agctaaccct aattgctata
      241 tctggtatta ttgcatatat gccattatct acttatgatt ttattttaaa aaaagatgta
      301 ggaataggta ttaataattt aaaactatat aaatattctt ggatagcaag ttctattgct
      361 agcttacttg gctttggagg agccacttct ctagctttta aacaatattt ttatggaaat
      421 catgttgagg ataaaaaaaa gcttttaaaa gaaattggga aaatcatatt tttaaattta
      481 actggattat ctatattatg tttaatttat cttcttttag aatttaaaga tatacaaaat
      541 ttagggatta taaaatatgt agtatatgga gtttctgcat atgcaccatt aattattata
      601 aattcactat atagatataa gataaaaaaa gaaaaagatg aaataatttt aaaagttgtg
      661 tgtatatctt ttatagagtg gacattaaac atgctactta tatacttaat attaattatt
      721 acaggtgctc atattaaact tgcaaaatat ttttgtgttt atgttagagc aacagcagtt
      781 gcaatagtaa gtatggttcc aggtggtatc ggcacatttg atttaacatt tatagatggc
      841 tttaaagcct taggagtacc catagagcaa acctttttgg taataatact ctataggata
      901 agttatttca tattgccagc aggaataggg gtaattctat atattcatga gtttggaatg
      961 aatttcaaag ccttaataaa aattagaaat aataaaaatt gagtttataa aaataatctt
     1021 ttaaaaactt gtaaatctaa aaatataaat aagtattaca tttttgtaat gaaaatgaaa
     1081 tattaataaa tatatttgaa gaaaggtagg gtgtataata aatttggatt ttaaacatag
     1141 ggaggacaaa aggtgaagtt aaatataaag ataaatg
//
LOCUS       DAEVGI010000308         1169 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEVGI010000308 DAEVGI010000000
VERSION     DAEVGI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.310"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(120..560)
                     /locus_tag="K8M77_002593"
     CDS             complement(120..560)
                     /locus_tag="K8M77_002593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087017.1"
                     /translation="MFNLYNEFLKYEYNELNELFKMAKTKEEQDFYIALCNFKLQKEQ
                     KKVIGNDFEIEVTLNEILNWVECETFRLNLPDSKTFYFSSEKSNDNYDFKIFKCFSSD
                     KDIKNLKNIIRSEIESVNNEYSVCKIIVNVRFGSLTFYFRNKKI"
     gene            complement(810..>1169)
                     /locus_tag="K8M77_002594"
     CDS             complement(810..>1169)
                     /locus_tag="K8M77_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087018.1"
                     /translation="LEDRTIKLVNTKGGRELTREIPNDRVELVKEVLEHNYHEDRLFS
                     INGDSINRYLNRIEDKLGIERHSNHDIRRLIAQEKFDSYRKSGLSIKDAANKTSAWLS
                     HGDNRNELLEKSYIKLK"
BASE COUNT          417 a          168 c           90 g          494 t
ORIGIN      
        1 catagaaaat ttggcttttt tctatacaaa aaaataatgt ataaaaaatt accgtttttg
       61 atactaataa taaatttttt aaattttaaa tatataagaa caccaaaaga aagtaaattt
      121 tatatttttt tatttctaaa ataaaaagtt aatgatccaa atcttacatt tactattatt
      181 ttgcaaacac tatattcatt attaacactt tctatttcag atcttattat attttttaaa
      241 tttttaatat ctttatcaga gctaaaacat ttaaatattt taaaatcata attatcattg
      301 cttttttcac ttgaaaaata aaaagtttta ctatctggta gatttaatct aaatgtttca
      361 cactcaaccc aatttaatat ttcatttaag gttacctcaa tttcaaagtc atttccaata
      421 actttctttt gttctttttg taatttaaag ttacacaatg ctatatagaa gtcctgttct
      481 tctttagttt tagccatttt aaacagctca tttaattcgt tatattcata ttttaaaaat
      541 tcattataca aattaaacat aatatttaac tccttaaaat taattaatat aaaaagaaga
      601 tagaccctat attttttcaa tgtcctcaaa ataaggaact tcataaataa atataaaatt
      661 tatcttcttt ttagtgtttt ggtaatgaat gtttgcaatt ttaaaataaa aagtaaatac
      721 tagattttca attaccagta aacaaaatca cagtattaat ataaatttta aattaagata
      781 agattttaaa caataaaaac cagaagaatt tattttagtt ttatatagct cttttcaaga
      841 agctcatttc tattatctcc atgacttaac caagcacttg ttttattagc tgcatctttg
      901 atacttaaac cagattttct ataactatca aatttttctt gagctattaa tcgccttata
      961 tcatggttac tgtgcctttc tattcctagc ttgtcctcta ttctatttaa atatctattt
     1021 atagaatctc catttatact aaataatcta tcttcgtggt aattatgttc taatacttct
     1081 ttaacaagct ctactctatc atttggaatt tctcttgtaa gttctcttcc cccttttgta
     1141 tttacaagtt ttatagttct atcctctaa
//
LOCUS       DAEVGI010000309         1155 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEVGI010000309 DAEVGI010000000
VERSION     DAEVGI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.311"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            268..993
                     /locus_tag="K8M77_002595"
     CDS             268..993
                     /locus_tag="K8M77_002595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087019.1"
                     /translation="MKNKKKKNFLLEKKNDYIIYIHSLVIRTESYKSSLKEAIDKLED
                     FIKNEKLKNKNIKESEIKVDYKIYNNFRKRLMGDEGYLYNLIGDGQKSAISYFKFRSI
                     IKKRKSNNSLGFEIRDIEENVEKHLKEMNRFRNWANHVPESLLVSEMELIKEDGINYE
                     IKGDIVIYNFRYCSLEYMIQLLKESTNIYNIVVEVHQSMKKDLGSLIGEHIYIKKDYQ
                     NIVKGVKSKSNEIARRSLIIQNS"
BASE COUNT          529 a           59 c          169 g          398 t
ORIGIN      
        1 gaattttatg taattttatc atataattgt aaatataaga taaaattaaa tatggcttaa
       61 aaattaactt gaaagttaat taatacttat atagtttata ttaatatgta taagtatgaa
      121 atgaatatag atacattaaa agtaaattga taaattatta aataaatata tagatagtta
      181 ataacatctg ataaaatacc aattactaat aaaatagaga aggtagtata tatacaatat
      241 tgataattac taagggggaa atatataatg aaaaacaaaa agaaaaaaaa ttttttgtta
      301 gaaaaaaaga atgattatat tatatatatt cattctttag taataaggac agaatcatac
      361 aaatcaagtt tgaaagaggc tatagataaa ttagaagatt ttattaaaaa tgaaaaacta
      421 aaaaataaaa atataaaaga aagtgaaatt aaagttgatt ataaaatata taataatttt
      481 agaaaaagat taatgggaga tgaaggatat ttatataatt taattggaga tgggcaaaaa
      541 agcgctatat cttattttaa gtttaggagt attataaaaa aaagaaaaag taataattct
      601 cttggatttg aaattagaga tatagaagaa aatgtggaaa aacatttgaa ggaaatgaat
      661 agattcagaa attgggctaa tcatgtgcca gaatcattac tagtatcaga aatggagctt
      721 attaaagaag atggtattaa ttatgaaata aaaggtgata ttgttattta taattttaga
      781 tattgttctt tagaatatat gatacaatta ttaaaagaaa gtactaatat ttataatata
      841 gttgttgaag tacatcaaag tatgaaaaag gatctcggaa gtttaattgg ggaacatata
      901 tatattaaga aagattatca aaatatagtt aagggagtaa aatctaagtc taatgaaatt
      961 gctagaagat cattaattat acaaaattca tagaatgtat taatattttt attaatttga
     1021 tgatagagtt gttataaatg tcaaatggat taacaactaa gagatttaaa gataagaata
     1081 ttgatgtttg ataaaacagt ttaaactatt aagtttaagc tgttttttat ttttaatgaa
     1141 ctttttagaa ttttt
//
LOCUS       DAEVGI010000310         1155 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEVGI010000310 DAEVGI010000000
VERSION     DAEVGI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.312"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            51..452
                     /locus_tag="K8M77_002596"
                     /pseudo
     CDS             51..452
                     /locus_tag="K8M77_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            602..1114
                     /locus_tag="K8M77_002597"
     CDS             602..1114
                     /locus_tag="K8M77_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBI7087020.1"
                     /translation="MLSENLLQRLNEQVNFEFYSSYTYLAMAAYAESVDLSGFANFFR
                     VQAQEELFHAMKFYDYIFQKNGVVELEQIDKPKFNFANIIEAFEKGYEHEQLVTSKIY
                     GLADIAFEEKEHATMSLLKWFIDEQVEEENNFHSLLKKVRRSEGNPAALYMMDDELAA
                     RVFTPPTTTK"
BASE COUNT          469 a          138 c          159 g          389 t
ORIGIN      
        1 ttaggtatgt tgcccaagaa tcttggcatt tcaatgccga gtagttcaaa aaatcagata
       61 tacttcaaat tttaattcat attaagagaa accatattga gcaaaactca tcattaataa
      121 aaataaaaga caataataaa atactaatgg taagagaaat aataaactat ctaaaagaaa
      181 actataaaga agaaatttca atcgatcata tatgtaacca tgttgctttt agtaaatatt
      241 atacatgccg tacctttaaa aaggtaacag ggcaaactat tttagattat attaatcata
      301 ttagatgtat taatgctaga aatttaatcc tctataatgg atacactata tctgaaagtg
      361 cttataaaag cggctttaat aacttatctt atttttcaaa aacttataaa aagtatatgg
      421 gattacttcc atctgaagaa aaaaatacat aatttaagta ttataaagta atctgaagat
      481 tctgccatct attattaata gactaataat ttctgttaat aatttttata aattagtttg
      541 cttaatttta tttttaagac tacaattatt ttatagaaat aataaaaaga ggtgtacata
      601 tatgttaagt gaaaatttat tacaaagact taatgaacaa gttaattttg aattctattc
      661 atcatatact tatttagcta tggctgctta tgctgagtca gttgacttaa gcggttttgc
      721 taactttttt agagttcaag ctcaagaaga attattccat gcaatgaaat tttatgatta
      781 tatattccaa aaaaatggcg ttgttgaatt agaacaaata gataaaccta aatttaattt
      841 tgcaaatata attgaagcct ttgaaaaagg ttatgaacat gaacaattag taactagtaa
      901 aatatatggt cttgcagata ttgcttttga agaaaaagaa catgcaacta tgagtttact
      961 taaatggttt atagatgaac aagttgaaga agaaaacaac ttccactcat tattaaagaa
     1021 agttagaaga tctgaaggta accctgctgc cttatatatg atggatgatg aattagcagc
     1081 aagagtattc actcctccaa caactacaaa atagatttaa tttaaagtta tcaataaaaa
     1141 gggcgtgtat caaaa
//
LOCUS       DAEVGI010000311         1147 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEVGI010000311 DAEVGI010000000
VERSION     DAEVGI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.313"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..599
                     /locus_tag="K8M77_002598"
     CDS             <1..599
                     /locus_tag="K8M77_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI7087021.1"
                     /translation="ETIIILTILFLISILLVLFYLKKARDSKLDSLKDGLCNIYNRRF
                     LDSYINNLKEKDLPISFLMIDVDYFKLYNDNYGHQAGDFVLKSISSVLKRNSRKEDIV
                     SRYGGEEFCVLLKGASKHSSINYAKRIKENLDNLNIKHKYSKISNNVTFSIGIYTTYT
                     KNDLKNAIKLSDKALYISKTRGRNTYTYLEDNSSDSSN"
BASE COUNT          440 a          144 c          133 g          430 t
ORIGIN      
        1 aagaaacaat tattatttta acaattctat ttttaatctc tattttatta gtgctttttt
       61 acttaaagaa agctcgggat tcaaaattag attctttaaa agatggactt tgcaatattt
      121 acaataggcg ttttttagac tcttatataa ataatttaaa agaaaaggat ctacctattt
      181 cttttttaat gatagatgta gattatttta aactttataa tgataactat ggtcatcaag
      241 caggtgattt tgtgctaaaa agcatatctt ctgtattaaa aagaaattct cgtaaggaag
      301 atatagtttc acgttatgga ggagaagaat tttgtgtttt actaaaagga gcttctaaac
      361 attcttctat taattacgct aaaagaatca aagaaaattt agataattta aatataaagc
      421 ataaatactc aaaaatttct aacaatgtaa cctttagtat aggaatatat actacatata
      481 ctaaaaacga tctaaaaaat gctattaaac tttctgataa agcactatat atatctaaaa
      541 caagagggag aaatacatat acttatctag aagataactc ttctgattct tctaattaac
      601 ttaatataag gaatggcgtg tatcaaaata acttatttaa aacttacaaa aatatttcga
      661 gaattactag attttaagtg aaagtccaag ttttgaattt ggagtcttga acttatgcaa
      721 tccaactttg tgagattgta ttcctaagga attaacagtc tcagcttttt aacttgatat
      781 tttctacgta ataatttaaa ttataaagct catctagata ttatcttcat agggtttgta
      841 agttttatta tattttgata cagcctcttt cttatataat taaggattac tattaatttt
      901 tttggagaga actcttagtc tacttaaaag cttttggaaa ttatattttc taataatata
      961 gtttactcta aaaaatacct atgtatcttt taaatttttc ttaaccttaa aaacagtaat
     1021 acctaataga aaagaattta ttatgctaat ccaccccaaa tatgaacctt ctataagaaa
     1081 aattatagtt actataaaaa aatatactac tcctaaaatc cataaatatc tttgattttc
     1141 taacgac
//
LOCUS       DAEVGI010000312         1136 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEVGI010000312 DAEVGI010000000
VERSION     DAEVGI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.314"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            43..516
                     /locus_tag="K8M77_002599"
     CDS             43..516
                     /locus_tag="K8M77_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBI7087022.1"
                     /translation="MEKTKECKNLLLKEILMDFLFIISMVALILLIDVVLGQLIDIYI
                     KLGGKFIGTNVINESVSLRIIFASIVGPIIESFLIIGLINLSKKIIKSKFKYSLLVAL
                     IFALLHYYSLIYIFCVIPSAIIMVFSYTYYKPKKLSPFWILTLIHMINNFIITMS"
     gene            558..1038
                     /locus_tag="K8M77_002600"
                     /pseudo
     CDS             558..1038
                     /locus_tag="K8M77_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459116.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          477 a           91 c          173 g          395 t
ORIGIN      
        1 ttgttttaaa gatttatact attaatgaaa agaggattta tgatggaaaa aacaaaagaa
       61 tgtaagaact tattattaaa agaaatacta atggattttt tatttattat ctctatggtt
      121 gcacttatat tattaattga tgttgtttta ggacaattaa tagatatata tattaaatta
      181 ggtggaaaat ttattggaac caatgtaatt aatgagagtg ttagtttaag aattattttt
      241 gctagtatag taggacccat aatagagtca tttttaataa ttggattaat aaatttaagc
      301 aaaaaaatta ttaaaagcaa atttaaatat tcattattag ttgcactaat atttgcttta
      361 ttgcattact acagtctaat atatattttt tgtgtgatac ctagtgcaat aataatggta
      421 ttttcttata catattataa acctaaaaaa ttatctccat tttggatttt aaccttaata
      481 cacatgataa ataattttat aattactatg agttaagcta gaatttatat tttaaaattt
      541 actctaagga gggtaaaatg aaatacaaaa taagatttgc agatacagaa gattacaaga
      601 tgataaatga aattattaga gaggtacatg acttacatgt cgaaaataga caagatgtgt
      661 acaatgaaac cgataaacca ttatcagaag aggaatttaa agaaattcta gagaatgata
      721 gatacaagat gtttttagtt taggaaagag aaagtaaaga ggttatagct tttagtctta
      781 tacaaatagt agggcctaga aacatacaaa tacttactct agtaaaaact gacttaattg
      841 atactttttg tgttaaggaa aagtatcgta aaagaggaat aggaaaattt ttgtttcagc
      901 atattataag ttttgcaaaa aaaagaagga gtaaacacat tacaattagt tgtctgggaa
      961 tttaataaag gagctattaa attttatgaa gctttgggga tgaaaaaagg aaatataagt
     1021 atggaattag ctttatagaa aaataaaact aagtatgtaa aataatatta actaaagttt
     1081 aaaataactt acaataagat gattgaactt ttcaatatta aaaatatagt agaata
//
LOCUS       DAEVGI010000313         1133 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEVGI010000313 DAEVGI010000000
VERSION     DAEVGI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.315"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1012
                     /locus_tag="K8M77_002601"
     CDS             <1..1012
                     /locus_tag="K8M77_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBI7087023.1"
                     /translation="IPENSKKTGVVTASKGLNVRKEANTSSKIIGILNSGESVEIIGE
                     ENGFYKITYKGQEAYASKNYINIFNSNSNVNPGLDIGNASKTNYGVSLNEYIKLQQRN
                     NPSNYSHSELEKYINPAKATNKLQFLRIDKFRSVNVSGLISRLSNKGVLTGQGQAFIN
                     AAKAFNIDPIYLVSQCLHETGNGTSKLAKGVTITEIADESRPIYNGTGQLVGYHMIKL
                     SKPVTVYNLFGIGAKDNSSVFPNRALILGTTYAYNRGWTSIENAIKGAAEFVSLNYVH
                     SSRYSQNTLYKMRYNQNVSNIWHQYATTPWYASSIADIMRSYQDLYLENNFTFDVPVF
                     EG"
BASE COUNT          466 a          129 c          179 g          359 t
ORIGIN      
        1 tattcctgaa aatagtaaga aaactggcgt tgtaactgca tctaaaggat taaatgtaag
       61 aaaagaagct aatacttcat ctaaaattat tggaatttta aatagtggag aaagtgttga
      121 aataatagga gaagaaaatg gtttctataa gataacttat aaaggacaag aagcttatgc
      181 atctaaaaat tatataaata tttttaatag taactcaaat gttaatccag gattagatat
      241 aggaaatgct tcaaaaacaa attatggagt atcacttaac gaatatataa aattacaaca
      301 aagaaataac ccttcaaatt attcgcattc agaattagaa aaatatataa atccagctaa
      361 agctactaat aagctacagt ttttaagaat agataaattt agatcagtta atgtaagtgg
      421 attaattagt agattaagta acaaaggtgt tttaacagga caaggacaag cttttataaa
      481 tgctgctaaa gcctttaaca tagatcctat ttacttagtt tctcagtgct tgcatgaaac
      541 tggtaatgga acaagcaaac ttgcaaaggg cgtaacaatt actgaaattg cagatgaaag
      601 tagacctata tataatggta ctggtcaatt agtaggatat catatgatta aattatctaa
      661 gccagtaaca gtttataatt tatttggaat aggggctaag gataattcat cagttttccc
      721 aaatagagct ttaatattag gaacaacata tgcttataat agaggttgga caagtattga
      781 aaatgctata aagggtgctg cagaatttgt ttcattaaat tatgttcata gttcaagata
      841 tagtcaaaat actctttata agatgagata taatcaaaat gtatcaaata tatggcatca
      901 atatgctaca acaccatggt atgcatcaag tattgctgat attatgagga gttatcaaga
      961 tttatattta gaaaataatt tcacatttga tgtacctgtt tttgaaggat aataattata
     1021 atacaattaa aggattgtat caaaataact tattaataaa acttacaaac agcatgaaaa
     1081 aatatctaga tcagctttag aatttaaatt attacgtaga aaatatcacg tta
//
LOCUS       DAEVGI010000314         1132 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEVGI010000314 DAEVGI010000000
VERSION     DAEVGI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.316"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(412..681)
                     /locus_tag="K8M77_002602"
     CDS             complement(412..681)
                     /locus_tag="K8M77_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HBI7087024.1"
                     /translation="MNSEKKKALQCLKTAKGQIEGIIKMIEDERYCIDISNQVMASQA
                     LLKKANKLILQQHMHNCVKGAFENNESKDEKIEEILSVLNKLIDK"
BASE COUNT          396 a          181 c           69 g          486 t
ORIGIN      
        1 tacttatagt taataaatta tctcttattg ctctaaaatc actttgaatt gcttaaaaaa
       61 taaatttctt taaacccgcc aaaacaatag atccagataa ctttatatta tttataaaat
      121 cactcacatc attagatcaa taatattttt attttaaata agttaaaata ttatagtaat
      181 tattctaaat tcgctttaag ctactcaaat aaaaaaattt gaatctccaa aaacaacaga
      241 tctaaataat tttaccttaa taaagttaag taaatcatta gaataattta ttaatataga
      301 gaaccctaaa tttgctttta gggattcaac taaactaaat tgaaactcta gaaacaacag
      361 atttaaagaa ctttatatta taataaaact aaatacatca ttagaaatat actatttatc
      421 tattagctta tttagtacac ttaatatttc ttcaattttt tcatctttag actcattatt
      481 ttcgaaagca cctttaacac aattatgcat atgttgttgc aatattaatt tattagcttt
      541 ctttaataat gcttgggaag ccattacttg atttgaaata tctatacaat atctctcatc
      601 ttcaatcatt ttgattattc cttcaatttg cccttttgct gtcttcaagc actgtaatgc
      661 tttctttttt tctgaattca tattcatcgc tccctataaa ttcacattca cccccactgg
      721 gtgtctgtaa ttatatgata actctctact tattttatgt caacatactt tttaatattt
      781 tatgaacata attaacattt aattaatatg ttggatttta tcaaaaatat ttctaataat
      841 ttaataaatt ataaatgcat ttctaaaaca aatctttttt aaacctcttt ttcaaaaagc
      901 tattctattc tttctacttc tccaattttc atatattata atttttcact tattctattc
      961 ctcctactta ttttattctt cccatttatt ttatttttcc tacctattct attcttccca
     1021 attatcttat tctccccaat tatcttattc tttcaaatta ttttattctt tcaaattatt
     1081 ttattcttta aaagaaaatc atctatttaa atatatgata tattaataat ta
//
LOCUS       DAEVGI010000315         1095 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEVGI010000315 DAEVGI010000000
VERSION     DAEVGI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.317"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(65..469)
                     /locus_tag="K8M77_002603"
     CDS             complement(65..469)
                     /locus_tag="K8M77_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBI7087025.1"
                     /translation="MRTLNRLTIFIIIIGCIFSILSPIKSSLNSKTNEILVVGLCSIF
                     IMFFGNIAPKIPFNRYMGLRLPWTIRDESTWKIAHRILGYVSFPIGIGMFALSFFFNI
                     EVIVITGILIWIIIPGIYSLFFYYKKFKGVNM"
     gene            complement(679..936)
                     /locus_tag="K8M77_002604"
     CDS             complement(679..936)
                     /locus_tag="K8M77_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI7087026.1"
                     /translation="MSKVFKALSDETRREILKLLSKKDMSAGEISDHFNMSKPSISKH
                     LEILRESELISSERKGQFIIYSINTSVIQEVLGNFLSLFTK"
BASE COUNT          425 a          164 c           91 g          415 t
ORIGIN      
        1 aaattatttc tgttatataa aaaattgctt atctatatga caagcaattt atattccact
       61 agatttacat atttacccct ttaaatttct tataataaaa aaataatgaa tatatacctg
      121 gtattataat ccaaatcaaa ataccagtga ttacaataac ttcaatgtta aagaaaaatg
      181 ataaagcaaa catccctatt ccaataggaa aagatacata tcctaatatt ctatgggcta
      241 tcttccaagt gctctcatcc cttattgtcc aaggtaatct taatcccata tacctattga
      301 aaggtatttt aggagctata tttccaaaga acataataaa tattgaacat aaaccaacaa
      361 ctaagatttc attagtttta gagtttaatg aacttttaat tggagataat atagaaaata
      421 tacaaccaat tattataata aaaattgtaa gtctatttaa agttctcata gttttaattt
      481 taacattctc ttctgataaa tttgcaattt taggtgcctt tatatcaaat ataattaaag
      541 caaaaatcaa caatcctcca ataatagaaa ctattaaatt actttcttta actaaaaaac
      601 ctccaattat tatcagtaat gatgttataa aaaataatat tcttgtaaaa tcaaatttct
      661 tcataaatat tctccccctt attttgtaaa caagctcaaa aaattcccaa gaacttcttg
      721 tattactgat gtgttaatag aataaattat aaattgacct tttctttcag aacttataag
      781 ctcagattct cttaatattt ctaaatgctt tgatatagat ggtttagaca tattaaaatg
      841 atcagaaatt tcaccagcac tcatgtcttt cttactaagc agttttaata tttctcttct
      901 tgtttcatcg cttaaagctt taaatacctt actcataaaa tatatccttt ctacaatata
      961 tagatattta gttgattagt taaatatcta tattactatt atacaaacgt ttttccctaa
     1021 aaataacatt taagataata ttttatataa agttcatttt tttcatacaa atttaaatta
     1081 tttcactaaa atatt
//
LOCUS       DAEVGI010000316         1091 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEVGI010000316 DAEVGI010000000
VERSION     DAEVGI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.318"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..902
                     /locus_tag="K8M77_002605"
     CDS             <1..902
                     /locus_tag="K8M77_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI7087027.1"
                     /translation="EARDDWYENKNSIYMFQGKTNNINCNYKVNEGKAALIRIEDVSA
                     GGVFSEDNNMEKMKYLSDYFKANNIVFHIAWIPRYINPEKNIDNDLLKNNNFENVHFI
                     NMLDHLINRGAIIGLHGYTHQHNKQISGLGVELKWNVNSNKNKVLKVVESSLKTAKTL
                     NIPIGFFESPHYKADRNQQKIIEQYFPVMFEPYAGYWNLNPLISFSNKSTLYVPAPLG
                     YVKDNGETVARRIRNYSNEILTAFFIHPYIFLGSIENKNNSTNNEEIYEFNENSPILK
                     IISALKERGCKTITVNELNNQII"
BASE COUNT          458 a          108 c          171 g          354 t
ORIGIN      
        1 ttgaagctag ggatgattgg tacgaaaata aaaactcaat ttatatgttt cagggtaaaa
       61 ctaataatat aaactgcaat tataaagtta atgaaggaaa agctgcttta ataagaatag
      121 aggatgttag tgctggggga gttttttctg aagataataa tatggaaaaa atgaaatatt
      181 taagtgatta ttttaaagct aataatatag tatttcacat agcttggata cctagataca
      241 taaaccctga aaaaaacata gataatgatt tgcttaaaaa taataatttt gaaaatgttc
      301 attttataaa tatgctagat catttaataa atagaggggc tataattggt cttcatggat
      361 acactcatca acataataag caaataagtg gacttggagt agagttaaaa tggaatgtta
      421 atagtaataa aaataaagtt ttaaaggttg ttgaatcctc attaaagaca gctaagacct
      481 taaatatacc tataggtttc tttgaaagcc cacattataa agcagataga aatcagcaaa
      541 aaataataga acaatatttc ccagttatgt ttgaacctta tgcaggatat tggaacttaa
      601 atccattaat aagttttagt aacaagtcta cattatatgt acctgctcct ttaggatatg
      661 ttaaggacaa tggagagact gtagctagaa gaattagaaa ttatagcaat gaaattttaa
      721 cggcattttt catacatcca tatatttttt taggaagtat agagaataaa aataatagca
      781 caaataatga ggaaatatat gagtttaatg aaaattcacc tatattaaag ataatatcag
      841 cattaaaaga aagaggatgc aaaaccataa ctgttaatga attaaacaat caaattatat
      901 agtaaaagtt ttattatgta tttaagtgaa gcagataaat atggatataa tgaaaatgaa
      961 tctgtatatt atccataact tggggaaagt tctggttgga tacattatcc tatgaattaa
     1021 gtaaaattaa tatatttaca ttaaattcat agtataattt atataaatga ttgtcttaaa
     1081 aatactatta a
//
LOCUS       DAEVGI010000317         1090 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEVGI010000317 DAEVGI010000000
VERSION     DAEVGI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.319"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..>1090)
                     /locus_tag="K8M77_002606"
     rRNA            complement(<1..>1090)
                     /locus_tag="K8M77_002606"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          232 a          334 c          236 g          288 t
ORIGIN      
        1 cagccattgt agcacgtgtg tagccctaca cataaggggc atgatgattt gacgtcatcc
       61 ccaccttcct cctggttaac ccaggcagtc tcgctagagt cctcaactta atggtagtaa
      121 ctaacgacaa gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga
      181 cgacaaccat gcaccacctg tcaccttgtc cccgaaggga tttcctcgat taagagtaat
      241 gcaagggatg tcaagtgtag gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc
      301 cgctacttgt gcgggtcccc gtcaattcct ttgagtttta atcttgcgac cgtactcccc
      361 aggcggaata cttaatgcgt tagcggcggc acggaggtgt tgaaaccccc acacctagta
      421 ttcatcgttt acggcgtgga ctaccagggt atctaatcct gtttgctccc cacgctttcg
      481 agcctcagcg tcagttacag tccagagagt cgccttcgcc actggtgttc ttcctaatct
      541 ctacgcattt caccgctaca ctaggaattc cactctcctc tcctgcactc tagataacca
      601 gtttggaatg cagcacccaa gttgagcccg ggtatttcac atcccactta atcatccgcc
      661 tacgctccct ttacgcccag taaatccgga taacgctcgc cacctacgta ttaccgcggc
      721 tgctggcacg tagttagccg tggcttcctc cttgggtacc gtcattatct tccccaaaga
      781 cagagcttta cgatccgaaa accttcatca ctcacgcggc gttgctgcat cagggtttcc
      841 cccattgtgc aatattcccc actgctgcct cccgtaggag tctgggccgt gtctcagtcc
      901 caatgtggcc gatcaccctc tcaggtcggc tacgcatcgt cgccttggta ggccgttacc
      961 ccaccaacta gctaatgcgc cgcgggtcca tctcatagcg gattactcct ttggttgaat
     1021 gatgatgcca tctttcaaca ttatgcggta ttaatcttcc tttcggaagg ctattccact
     1081 ctatgaggca
//
LOCUS       DAEVGI010000318         1082 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEVGI010000318 DAEVGI010000000
VERSION     DAEVGI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.320"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..970)
                     /locus_tag="K8M77_002607"
     CDS             complement(<1..970)
                     /locus_tag="K8M77_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBI7087028.1"
                     /translation="MCTHIHISSVNNNLYWGRTLDTSFNPFEVGSKITIVPRNFTLQT
                     ESNPWKTKYSFLGINLCGSTLFFDGVNEKGLAGGLLFLKACTWDKKENIEKQGLTAIN
                     SGEIVPWILSNFESVSDIKNNISKVVVTPDDIPSLGELGKGNPITAHYTFTDKMGESV
                     VLEPTDHGHFRVFDNNVGVMANDPTFDWHMTNLANYLQVQGFNKQQNHLNSKTVITPI
                     SNGTGLLGLPGDYTSPSRFVRVAYLRNLIGDVTDEEAPARLFSILNSVWVPKDVERFY
                     EDKKESDFSSYMCAYDQKLGKLYLRVFNDIDTKEFSLENVKENELVT"
BASE COUNT          365 a          178 c          129 g          410 t
ORIGIN      
        1 aagttactaa ttcgttttct ttaacatttt ctaaagaaaa ttcttttgta tctatatcgt
       61 taaacacacg taaatataac ttacctaatt tttgatcata tgcacacata taacttgaaa
      121 aatctgattc ttttttatct tcataaaaac gttcaacatc ctttggaacc caaacagaat
      181 ttaaaatact aaataaacgt gctggagctt cttcgtcagt aacgtctcct ataagatttc
      241 ttaaatatgc aacacgtaca aatcttgatg gtgaagtata gtctccaggt aagccaagta
      301 agcctgtacc atttgaaatt ggtgtaataa ctgttttgct atttaaatga ttttgttgct
      361 tattaaatcc ttgaacttga agataatttg ctaaattagt catatgccaa tcaaatgttg
      421 gatcattagc cattactcct acattgttgt caaatacacg aaaatgacca tgatctgttg
      481 gttctaatac aactgattct cccattttat ctgtgaaagt ataatgagca gtaataggat
      541 ttccttttcc aagttctcct aatgaaggaa tatcatcagg tgttactaca acttttgata
      601 tgttattttt tatatcacta acactttcaa aattacttaa aatccaagga actatttctc
      661 cgctgttaat agctgtaagg ccttgttttt ctatattttc ttttttatcc catgtacatg
      721 cttttaaaaa taaaagtcct cctgcaagac ctttttcatt aactccatca aaaaataaag
      781 tagatccaca taaatttata cctaaaaaag aatatttagt tttccatggg ttactttctg
      841 tttgtaaagt aaaatttcta ggaactattg ttattttaga accaacttca aacggattaa
      901 atgatgtatc taaagttctt ccccagtaaa gattattatt aacacttgaa atatgtatgt
      961 gagtgcacat ttaaatcatc tccttaattt ttattatgtt cttagattat acctataata
     1021 ccctattgtc aatttaagct taatatagat atttatacta aaaataagaa atacttttat
     1081 tt
//
LOCUS       DAEVGI010000319         1082 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEVGI010000319 DAEVGI010000000
VERSION     DAEVGI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.321"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(87..1016)
                     /locus_tag="K8M77_002608"
     CDS             complement(87..1016)
                     /locus_tag="K8M77_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBI7087029.1"
                     /translation="MKIAIVGAGAMGSRYGYMLHEAGNEVILIDGWKDHVDTINKEGL
                     TVEENVEIKKVKIPAMLPQDAKEIPELIILFTKSMGLEPMLESIKGILGKNTKVLCLL
                     NGLGHNETVAKYVDNKNILMGVTLWTAGLKGPGHVVLTGSGSLEVQNIDPSMEKEAKE
                     ICDILSNAGLKAHYSENVLFSIWRKACVNGTLNSGCTILDCNIKEFGELKEASELIRN
                     IIKEFADIASKYGVKLDVEEVARGIEAIYDPSQAGAHYPSMHQDLIQNHRLTEIDYIN
                     GYVSRKGKEFNINTPYNDLLTTLVHAKEQLLIK"
BASE COUNT          325 a          206 c          115 g          436 t
ORIGIN      
        1 acgggtgttc ttgccaaagg cataaaaagt tgatttctat aaaacataaa aatcaacttt
       61 taaaaaacta tatttatagt atactcctat ttaataagta attgctcttt tgcatgaaca
      121 agagtagtaa gtaagtcatt atatggtgta tttatattaa actctttacc ttttcttgat
      181 acataaccat ttatataatc tatttctgta agacgatgat tttgtattaa atcttgatgc
      241 attgatggat agtgagcgcc agcttggctt ggatcatata ttgcctctat tcctcttgca
      301 acctcttcaa catctaactt cactccatat ttagatgcaa tatcagcaaa ctcttttata
      361 atattacgta taagctctga agcttctttt aattcaccaa attctttaat attacaatct
      421 aaaattgtgc atccactatt taaagttcca tttacgcaag ctttacgcca tattgaaaat
      481 aatacatttt cactatagtg agcttttaat ccagcattac ttaatatatc gcaaatttct
      541 tttgcctctt tttccatact tggatcaata ttttgaactt ctaggcttcc acttcctgtt
      601 aatacaacat gtcctggtcc ttttaacccc gctgtccata aagttactcc cataagtatg
      661 tttttgttat caacatattt tgctacagtt tcattatgtc ctaatccatt taataagcat
      721 aatacttttg tattttttcc taatatacct tttatagatt ctaacattgg ttctaatccc
      781 atagatttag taaataatat tattaactct ggaatttctt tagcatcttg aggtagcatt
      841 gctggtatct ttactttttt aatttctaca ttttcctcaa cagttaatcc ctctttgttt
      901 atagtatcta catgatcctt ccacccatct attaatataa cttcatttcc agcttcatgt
      961 agcatatatc cataacgtga gcccattgct cctgctccaa caattgcgat tttcatttaa
     1021 atcaactctc ctcttttatc ttttttggaa attaaatata tttaaaaata tttattattc
     1081 aa
//
LOCUS       DAEVGI010000320         1045 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEVGI010000320 DAEVGI010000000
VERSION     DAEVGI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.322"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            126..965
                     /locus_tag="K8M77_002609"
     CDS             126..965
                     /locus_tag="K8M77_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBI7087030.1"
                     /translation="MELFVSDLDGTLLNKDQVISDYSKKELNRLISTGVNFAIATARS
                     PATVLDILNGIDIKTPVVLMNGVIIFDIEKKKYIDVKEIDKESVKEIIKILKKYNKTF
                     FLYGIKDDYLWVYHKDFTYDFERKYYEERCNKKLKSFKKVENYLDILNDNQIINFVFF
                     EDNKLIAHELFEKIMKVKGVTGNCYKDIYNEGAFFLDVYNEEASKANGIKFLADYVEH
                     SKVITFGDGENDIPMFKISDECYALENSSQELKSISTGVIGNNNDDAVVKFLIERLKI
                     EKI"
BASE COUNT          442 a           68 c          165 g          370 t
ORIGIN      
        1 tattaataga ttattattaa ttttatatat tatttttcat aaactacttt gaataatata
       61 taaatatgtt gtatattaga taaaaaggta attaatgttt agaatgttaa agaaagaggt
      121 attaaatgga gttatttgtt tcagatttag atggaacttt attaaataag gatcaagtta
      181 tatcagatta ttctaaaaag gaattaaata gacttataag tacaggtgtt aactttgcaa
      241 tagcaacagc tagaagtcca gctactgttt tggacatttt aaatggaata gatataaaaa
      301 cacctgttgt tcttatgaat ggagttataa tttttgatat tgaaaagaaa aaatatatag
      361 atgttaaaga aatagataaa gaaagtgtaa aagagattat taaaatacta aaaaaatata
      421 ataaaacatt ttttctttat gggataaaag atgattatct atgggtttat cataaagatt
      481 tcacttatga ttttgagaga aaatattatg aggaaagatg caataaaaaa cttaaatctt
      541 ttaaaaaagt tgaaaattat ttggatatct taaatgataa tcaaataata aattttgtat
      601 tctttgaaga taataaactt atagcccatg aattatttga gaaaataatg aaagtaaaag
      661 gtgttactgg taattgttat aaagatatat ataatgaagg agcttttttc ttagatgtat
      721 ataatgagga ggcttcaaag gctaatggaa taaagttttt ggcagattat gtagagcata
      781 gtaaagttat aacttttggt gatggagaaa atgatattcc aatgtttaaa atctcagatg
      841 aatgctatgc tttagaaaat tcttcacagg aattaaagtc aatatcaact ggtgttattg
      901 gaaacaataa tgatgatgca gtagttaaat ttttaattga aagattaaaa atagaaaaaa
      961 tataaaatca acaaagaaaa tatagaagaa gtataattaa taggcgtatt tattttataa
     1021 tttatcaaaa tagagtttga atttt
//
LOCUS       DAEVGI010000321         1040 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEVGI010000321 DAEVGI010000000
VERSION     DAEVGI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.323"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            37..1032
                     /locus_tag="K8M77_002610"
     CDS             37..1032
                     /locus_tag="K8M77_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003456270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087031.1"
                     /translation="MQSRVNKINNHTVAALGIKSLDENLEKSIVNIINCNPEYIILGC
                     SEIPFTERILKALNNANCSKILILPFNYRFRNQREIVNEIKFMKNFPENNIAIILNSV
                     NDSNFILTDKICYVSSGSLNNNGIANNVNSYEIMNYKDCNYKLWESEIFDFINKEIIN
                     YRSNDQREKIEYILRELKKRLFINISNNSNILNEEYIYILLDILNSIDRFIMIGADVY
                     QYFDRDFLDIIKSNKKILAKINSIKNELFRILLNLNEGKNDINNKIFNCLKNDFLVLL
                     EELEELISLRENKYHNSIMSWRKDESISSFLLANNTINNSLIRKFYERIILNYER"
BASE COUNT          451 a           74 c          147 g          368 t
ORIGIN      
        1 ggtaattatc acctttaaat aggttgataa gtatttatgc aaagcagagt taataaaata
       61 aataatcata cagttgctgc tttaggaata aaaagtttag atgaaaattt agagaaaagt
      121 atagttaata taataaattg taatccagaa tatattattt tagggtgtag tgaaattcct
      181 tttacagaaa gaattttgaa agctttaaac aatgctaatt gttctaagat tttaatactt
      241 ccctttaact ataggtttag aaatcaaaga gagatagtta atgaaattaa atttatgaaa
      301 aatttccctg aaaataatat agcaattata ttaaattcag taaatgattc taattttatc
      361 ttaactgata aaatttgtta tgtatcttca ggaagtttaa ataataatgg tatagcaaac
      421 aatgtaaatt cctatgaaat aatgaattac aaagattgta attataaatt atgggaatca
      481 gaaatctttg attttataaa taaagaaata ataaattaca ggagtaatga tcaaagggag
      541 aaaatagaat acattttaag ggaacttaaa aaaaggttgt ttattaatat ttctaataat
      601 tccaatatat taaatgaaga gtatatatac attcttctag atatattaaa tagcatagat
      661 agatttatta tgataggagc tgatgtttat caatattttg atagagattt tttagacata
      721 ataaagagta ataaaaaaat attagctaaa ataaattcca taaaaaatga attatttaga
      781 atacttttaa accttaatga aggaaagaat gatattaata acaagatttt taattgctta
      841 aaaaatgatt ttttagttct tttagaagaa ttagaggagt taataagttt aagagaaaat
      901 aaatatcata attctattat gtcatggaga aaggatgaaa gtataagctc atttttatta
      961 gctaataata caattaataa ttctttgatt aggaagtttt acgaaagaat tattttaaat
     1021 tacgaaagat aagtggaaag
//
LOCUS       DAEVGI010000322         1034 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEVGI010000322 DAEVGI010000000
VERSION     DAEVGI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.324"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1027
                     /locus_tag="K8M77_002611"
     CDS             <1..1027
                     /locus_tag="K8M77_002611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087032.1"
                     /translation="TIGSIKRDLFEKKDLELKKEAKLNNCIIDIENKDSFRNENIFKS
                     SIQSLKSLVSKPIKFKEHLNLVINSYNSIIEKLLIDIKLIEDEEEKILDSILEYIEEV
                     NKNISIIDDNSTININNKRVKMLNITVQDFEKNKINYRLKLKDYIEAIRERAIKELEK
                     NNSIEEIISNNITLFKLYDEIIGVENVEIKLYKIEENRQRIISWDEVSKNSGGEGFLS
                     AFVILTSLLSYMRKDENDIFSRKEDGKVLIMDNPFAQTSSSHLLKPLMDIAKKSNTQL
                     ICLTGLGGDSIYNRFDNIYVLNLVSSKLNNSIKYMKSEHIKGDKEEKTEIIVSSRLKI
                     EEQTSLF"
BASE COUNT          471 a           67 c          156 g          340 t
ORIGIN      
        1 tactatagga agtattaaaa gagatttatt tgaaaaaaaa gacttagagt taaaaaaaga
       61 ggctaaatta aataattgta taattgatat tgaaaataaa gatagtttta gaaatgaaaa
      121 tatatttaaa agttctatac aatctttaaa gtctttagta agtaaaccaa taaaatttaa
      181 agaacattta aatcttgtaa ttaattcata taattctatc attgaaaagt tattaattga
      241 tattaaatta attgaggatg aggaagagaa aatattggat agcattttag aatatattga
      301 agaggtcaat aaaaatattt ctataataga tgataattca actataaata taaataataa
      361 aagagtaaaa atgttaaata ttacagtaca ggattttgaa aaaaataaaa ttaattatag
      421 attaaaactt aaagattata ttgaagctat tagagaaaga gctattaagg aacttgagaa
      481 gaataattcc attgaagaaa taattagtaa taatataact ttatttaagt tatatgatga
      541 aattattgga gttgaaaatg ttgagattaa attatataaa atagaagaga atagacagag
      601 aataataagt tgggatgaag tttcaaaaaa ttcaggagga gaagggtttc tttcagcatt
      661 tgtaatatta actagtttac tatcttatat gagaaaagat gaaaatgaca tatttagtag
      721 aaaagaggat ggaaaagtat taataatgga taatcctttt gcacaaacaa gttcttcaca
      781 cttgttaaaa cctcttatgg atattgctaa aaaaagtaac actcaattaa tttgtctaac
      841 aggactaggt ggggattcta tttataacag atttgataat atatatgttt taaatttagt
      901 atcatcaaaa ttaaataata gtattaaata tatgaaaagt gagcatataa aaggagataa
      961 agaggagaaa actgaaatta ttgtttcatc tagattaaaa atagaagaac agactagttt
     1021 attttaaata atta
//
LOCUS       DAEVGI010000323         1021 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEVGI010000323 DAEVGI010000000
VERSION     DAEVGI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.325"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            157..813
                     /locus_tag="K8M77_002612"
     CDS             157..813
                     /locus_tag="K8M77_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI7087033.1"
                     /translation="MTQTQKNTQIFKYNKAEKRNKNFMYMDFRRGNCYNCDFSCSNFS
                     YASFRGAHFKSCDFFECKFDSTEFIGSNLKKSKFKKSKFKNVIFEGVNLDGVDFSGAT
                     FENVIFINSDISKTSGMKFKEDEVKIYEDMPSFEISDSLKEAALKALENKYIKKSRVL
                     DTKEGDLNTLSLIRLLENHKENMLIEGLKLSAEKIDREFCTLSYIDKAITKLKNEGLL
                     "
BASE COUNT          398 a          107 c          146 g          370 t
ORIGIN      
        1 ttaggtgtgt tgcccaagaa tcttggcatt tcaatgccga gtagttcaaa aagttatttt
       61 aaataaatta aaggtatttt tatttagaaa ggtggtggat acgttactat attggttaag
      121 tttataatat ataaataaga cttagtaacg attattatga cacaaactca aaaaaacact
      181 caaattttca aatacaataa ggcagaaaaa agaaataaaa attttatgta tatggatttt
      241 agaagaggaa actgctataa ctgtgatttt tcttgttcta attttagtta tgcaagcttt
      301 agaggtgcac attttaaatc ttgtgatttc tttgaatgca aatttgactc tacagaattt
      361 ataggtagta accttaagaa aagtaaattt aaaaaatcta aatttaaaaa tgtaatcttt
      421 gaaggggtta acttagatgg agttgatttt tcaggtgcta cctttgaaaa tgtaatattt
      481 ataaatagtg atataagtaa aacatctggc atgaagttta aagaagatga agttaaaatt
      541 tatgaagata tgccatcttt tgaaattagt gattctctta aggaagctgc tttaaaagct
      601 ttagaaaata aatacataaa aaaatctagg gttttagata caaaagaagg tgacttaaat
      661 accttaagtt taattagact tcttgaaaat cataaagaaa atatgcttat agaaggtctt
      721 aaactttctg ctgaaaaaat agatagagaa ttctgtactt taagctacat agacaaagct
      781 attactaagc ttaaaaatga aggtttatta tagaaattta tgaatagtta ggatataaag
      841 agagatttat aaactctctt tattttttat tgtaaaatac tattaagttt tatatccatg
      901 caatattttc ataaatacag cttatttacc taacataccc tataatatat atttatgcta
      961 taaatttcca tttattaata tttttataaa attccgttcc ctacttttaa taagtccaat
     1021 t
//
LOCUS       DAEVGI010000324         1020 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEVGI010000324 DAEVGI010000000
VERSION     DAEVGI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.326"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..>1020)
                     /locus_tag="K8M77_002613"
     rRNA            complement(<1..>1020)
                     /locus_tag="K8M77_002613"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          204 a          312 c          207 g          297 t
ORIGIN      
        1 tggaatatca accagttgtc catcacctac gcctttcggc ctcggcttag gtcccgacta
       61 accctcagcg gacgaacctt cctgaggaaa ccttaggttt tcggcctgtg ggattctcac
      121 ccacatctcg ctactgatgc caacattctc actcgtaacc agtccaccgc tccttacggt
      181 acgacttcag cccggttacg acgctctcct accgcttgaa ctaagttcaa gcccgtagct
      241 tcggtggtaa gtttagcccc gttacatttt cggcgcaaga tctctcgact agtgagctat
      301 tacgcactct tttaatgaat ggctgcttct aagccaacat cctagttgtc ttagaaatct
      361 cacatccttt cccacttaac ttacactttg ggaccttagc tgacgatctg ggctgtttcc
      421 cttttgacca cggatcttat cattcgtagt ctgactgccg agctcaaagt atgtggcatt
      481 cggagtttga taaggttcgg taagcgctat gccccctagc ccattcagtg ctctacctcc
      541 actactcata ctcgacgcta gccctaaagc tatttcggag agaaccagct atctccgggt
      601 tcgattggaa tttctccgct atccacagct catcccatgc tttttcaaca gcaacgtggt
      661 tcggtcctcc acgaggtttt accctcgctt caacctggcc atggataggt cacccggttt
      721 cgggtctacg gcatgcaact agtcgcccta ttcagactcg gtttcccttc ggctccgtac
      781 cttaagtact taaccttgct acataccgta actcgttggc tcgttctaca aaaagcacct
      841 catcaccctc ataaggggct ctgagcggtt gtaggcacac ggtttcaggt tctatttcac
      901 tcccctcccg gggttctttt cacctttccc tcacggtact gcttcactat cggtcatcag
      961 gtagtattta gccttgggag gtggtcctcc ctgcttccca caaggtttca cgtgtctcgt
//
LOCUS       DAEVGI010000325         1013 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEVGI010000325 DAEVGI010000000
VERSION     DAEVGI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.327"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            32..949
                     /locus_tag="K8M77_002614"
     CDS             32..949
                     /locus_tag="K8M77_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBI7087034.1"
                     /translation="MKKIMKMGLILSVLICLGAFVGCNSLNKKQVEATENKEAVETKD
                     TTNEKNTEGKDKAKTNELEYLPFDKDPNAEDGKVVSEYTNGLLKGTLQYPVRKDGKKI
                     VYLTFDDGPSTTNTPEVLKILDKYNIKGTFFVTGNSISAGDKSKELLKQIVKNGHAIG
                     NHTYSHDYKYLYPNRKMNVNNIMSDINKNENLMKEVLGEDFSTKVVRFPGGYWSWQDR
                     TPMKEKMIQEGIQNIDWNALNGDAEGKKKNADELVEFAKKSVEDLGPNADNVVVLMHD
                     TYGKEETVKALPRIIEYLQSKGFEFKSIK"
BASE COUNT          441 a          101 c          192 g          279 t
ORIGIN      
        1 agatcgaatc aattatatgg gggataaata tatgaagaaa ataatgaaaa tgggtcttat
       61 tcttagtgtg ttaatatgtt taggggcttt tgtaggatgt aattcactaa acaaaaagca
      121 ggttgaggca acagaaaata aagaagctgt agaaactaaa gatacaacaa atgaaaaaaa
      181 tacagaaggt aaagataagg caaagacaaa tgaattagag tatttacctt ttgataaaga
      241 tccgaatgct gaggatggga aagttgtatc agagtacact aatggtcttt taaagggaac
      301 attacaatat ccagttagaa aagatggtaa aaagatagtg tatttaactt ttgatgatgg
      361 tccatcaaca actaatacac cagaggtttt aaagatttta gataaatata atataaaagg
      421 aactttcttt gtaacaggaa attctataag tgctggagat aaatcaaaag aattattaaa
      481 gcagattgtt aaaaatggac atgctatagg aaatcataca tatagtcacg attacaaata
      541 tctttatcct aatagaaaga tgaatgtaaa taacataatg tcagatataa ataagaatga
      601 aaacttaatg aaagaagtct taggtgaaga tttctcaaca aaagtagtta gattcccagg
      661 tggatactgg tcttggcaag atagaacacc tatgaaggaa aaaatgattc aagagggaat
      721 acaaaacatt gattggaatg ccttaaatgg agatgcagaa ggaaaaaaga aaaatgcaga
      781 tgaattagtt gaatttgcta agaaaagtgt agaagactta ggccctaatg cagacaatgt
      841 agtagtttta atgcatgata cttatggaaa agaagaaaca gttaaggctt taccaagaat
      901 aatagagtac ttacaaagca aaggctttga atttaagagc attaaataaa tatattataa
      961 tttttacgaa caataattaa ttatttaata aaaattgtaa aacaagtatt aaa
//
LOCUS       DAEVGI010000326         1008 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEVGI010000326 DAEVGI010000000
VERSION     DAEVGI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.328"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(191..514)
                     /locus_tag="K8M77_002615"
     CDS             complement(191..514)
                     /locus_tag="K8M77_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087035.1"
                     /translation="MALGYLSVLYLFILVLSILGISLLFFLKNSKLKNVVFYFLVIWS
                     IFITYINATSLPTNYLAQQIIAWLFGSISIIAIIIKVKKTGKTNIPYILVTISVLLGI
                     FMMFF"
     gene            820..>1008
                     /locus_tag="K8M77_002616"
     CDS             820..>1008
                     /locus_tag="K8M77_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087036.1"
                     /translation="MKVCPNCKENFPYMDLMVALWGFVGCGYIRCKNCNTQYKVTKNS
                     KVFIALLALFPYFIEGYIF"
BASE COUNT          405 a          115 c           90 g          398 t
ORIGIN      
        1 aaaaaaagtc tgtaacaata atatattttt acagactttt tttgctagga tttattgtat
       61 aaactaaaat tttaaaacct attattttaa gaattttcaa tttaatatct cttttaagaa
      121 atttttattc ctatatactc ttaaaaaact taatttcaaa ccactattta ccataaatat
      181 atttaactaa ttaaaaaaac atcataaata ttccaagtaa aactgaaatt gttacaagta
      241 tatatggaat atttgttttc cccgtctttt taactttaat aataattgca attattgata
      301 tagatccaaa taaccaggct ataatttgtt gcgctaaata atttgttggc aaactagttg
      361 cattaatgta tgttataaaa attgaccata taactaaaaa ataaaatact acatttttta
      421 gtttgctgtt tttcaagaaa aaaagcaaag aaattcctaa tatacttaat actaatataa
      481 ataaatataa tactgataaa tatccaagtg ccatatttct accctccttt aataatgttt
      541 ttctatactt ttataatatc ctgaatttat taaaacaatt atgaagttaa tttataatct
      601 attcaaatgt taacttatac ttttaattaa taccataaat attcacttaa atttctttaa
      661 attttattaa ttaattaaaa aacagtgtat aatatattta taatatatgg tatatcttac
      721 ataaatatta tataaaacta aagttaaaga caataaagta atcaataata ttaaattaag
      781 atactttaat taacataaac tatttaagga aatgctctta tgaaagtttg tcctaattgc
      841 aaagaaaact ttccatatat ggatttaatg gttgcattat ggggatttgt tggttgtggg
      901 tatattagat gtaaaaattg taatactcaa tacaaggtta caaaaaattc taaagtattc
      961 attgccttat tagccttatt cccttatttt atagaaggct atatattt
//
LOCUS       DAEVGI010000327          977 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEVGI010000327 DAEVGI010000000
VERSION     DAEVGI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.329"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(24..716)
                     /gene="sfsA"
                     /locus_tag="K8M77_002617"
     CDS             complement(24..716)
                     /gene="sfsA"
                     /locus_tag="K8M77_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBI7087037.1"
                     /translation="MKYNSVIRKAIFLRRPNRFQAYVVLDDEELLVHVPNTGRCREIL
                     KEGCTVLLRKGTTPNRKTPYDLIAAYKGEVLINIDSQIPNKVVEEALRNKKIEKLVNF
                     NNISREKTFGNSRFDFKLQDDNENIYFLEVKGVTLEENGETRFPDAPTERGKKHILEL
                     IEIKKLGMGAGIIFLIQIDNVNKFSPNDETDPKFGEVLRLAKKEGVDIFAYNCKVTEE
                     EIELLNSVEIVL"
BASE COUNT          317 a          179 c           68 g          413 t
ORIGIN      
        1 cttttcaatt ctataaatta aacttataaa actatttcta cagaattaag taactcaatt
       61 tcctcttcag taactttgca attatatgca aagatatcta ctccttcttt tttagctaat
      121 ctcaatactt ccccaaactt aggatctgtt tcatcattag gagaaaactt atttacatta
      181 tctatctgta ttaaaaaaat tattcctgca cccattccta attttttaat ttcaatcagt
      241 tctaatatgt gtttctttcc tctttcagtt ggagcatctg gaaacctagt ctctccattt
      301 tcctctaaag ttaccccttt tacttctaaa aagtatatat tttcattatc atcttgtaat
      361 ttaaaatcaa atctagaatt tccaaaagtt ttttctctac ttatattatt aaaatttact
      421 aacttttcta tctttttatt tcttaatgcc tcttctacaa ctttattagg aatctgagaa
      481 tctatattta ttaaaacctc tcccttataa gctgcaatta aatcgtaagg agtttttcta
      541 tttggagtag ttccctttct taaaagaact gtacatcctt cttttaaaat ctctctacac
      601 cttccagtgt taggaacatg aactaataac tcctcatcat ctaaaactac ataagcttga
      661 aatctatttg gtcttcttaa aaaaatagct tttctaataa cactattgta cttcatctat
      721 agtaacctct tcatatatta tttaactctt acttttagtt ataaatcaac aaaaattttt
      781 attcctaaag tttatgtttt aatttctcta aataagtttt tctcataact caccaatttt
      841 ttattcctaa gttatattga aatttatatc aattggttct tattataact catcttataa
      901 ctcatcaact ttttattcct tatctactaa aaaacttaaa tatttctcag ttaataaaaa
      961 cataaaattt aaatttt
//
LOCUS       DAEVGI010000328          976 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEVGI010000328 DAEVGI010000000
VERSION     DAEVGI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.330"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            81..959
                     /locus_tag="K8M77_002618"
     CDS             81..959
                     /locus_tag="K8M77_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBI7087038.1"
                     /translation="MFKKILAIISTVTVLSFAGCGAQKNNNISKSSAESEYSGMSVTY
                     LNVGQGDSELIQVNGINMLIDAGTNAGANDLVKDLKNRGIKTIDIAIATHPHEDHIGG
                     MDEVLENFDVKSFYAPKVAHTTKTYENMLKAVKNEGLKIQQIKEGTKIDLGKDTEVQV
                     YSPVKSQYEELNNYSPVMKISYGQNSFMFTGDAESLVEKEILNENKDLKADVLKLGHH
                     GSHSSTSEEFLKAVNPSIAVVSCAKDNKYGHPHKETMSNLKKAGITVYETFRDGDITI
                     SSNGKKLDVKLHSENK"
BASE COUNT          412 a           97 c          185 g          282 t
ORIGIN      
        1 atttttttaa aatcaatatt ttaaattaca aaaaaataaa aatgtgttaa tattataaaa
       61 aaaggattct tggagggaaa atgtttaaaa aaatattagc aataataagt acagttacag
      121 ttttaagctt tgcaggatgt ggagctcaga aaaataataa tattagtaag agttctgcag
      181 aaagtgaata tagcggaatg agtgttacat atttaaatgt tggacaaggt gatagtgagt
      241 taattcaggt aaatggaatt aatatgttaa ttgatgcagg aactaatgca ggagctaacg
      301 acttagttaa agatttaaaa aatagaggca taaaaactat agatatagct atagctactc
      361 atcctcatga agatcatata gggggaatgg atgaagtttt agaaaatttt gatgttaaat
      421 ctttttatgc gcctaaggta gcacatacaa caaaaactta tgaaaatatg ttaaaggcag
      481 taaaaaatga gggattaaaa atacagcaga taaaagaagg aacaaagatt gatttgggaa
      541 aggatacaga ggttcaggtt tattcgccag ttaaatctca gtatgaagaa ttaaataact
      601 attcaccagt aatgaaaata tcctatggac aaaattcctt tatgtttact ggagatgcag
      661 aaagtttagt agaaaaagaa attttaaatg aaaataagga cttaaaggct gatgtgctta
      721 agttaggaca tcacgggtca cattcatcaa ctagtgagga gtttttaaaa gctgttaatc
      781 caagcatagc tgtagtttct tgcgctaaag acaataaata tggtcatcct cacaaagaaa
      841 ctatgagtaa tcttaaaaaa gctggtataa cagtttatga aacttttaga gatggagata
      901 taacaataag ttctaatggg aaaaagttag atgtaaaatt acatagcgaa aataaataat
      961 aaaaggtaaa taaaaa
//
LOCUS       DAEVGI010000329          970 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEVGI010000329 DAEVGI010000000
VERSION     DAEVGI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.331"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            39..350
                     /locus_tag="K8M77_002619"
     CDS             39..350
                     /locus_tag="K8M77_002619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087039.1"
                     /translation="MDECLYCGKNKFEYMYSLKHWTNGKYCSLRCLYKDIESTEFALN
                     FYSNEIESIRRDILNEKSKEEVNKLNQELDNSKHKRVVLLQKLTLMKSLIKSGQLKYN
                     K"
     gene            523..837
                     /locus_tag="K8M77_002620"
                     /pseudo
     CDS             523..837
                     /locus_tag="K8M77_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
BASE COUNT          416 a           65 c          135 g          354 t
ORIGIN      
        1 ataatttaat gtactattat atggatggtg atttatttat ggatgaatgt ttatattgtg
       61 gaaaaaataa gtttgaatat atgtattctt taaaacattg gacaaatgga aaatattgct
      121 ctttgagatg tttatataag gatattgaat ctactgaatt tgctttaaat ttttatagca
      181 atgaaattga atctattaga agagatatat taaatgagaa atctaaagaa gaagtaaata
      241 aattaaatca agaattagat aactctaaac ataaaagagt tgtattatta cagaaattaa
      301 ctttgatgaa gtcattaata aagtctggtc agttaaaata taataaatag ttaagggaag
      361 tgcttagata gcaaattatt tgctatcttt ttgttttatg gaaattaaaa aaggttataa
      421 ataatgaaat agtaaaagta taagaataga ttttaatatt ttaattttaa aattaaattg
      481 ttatattcat atataagact atattttagg gtaatatttt tatcagttca ttatcttgta
      541 gaatgttatc ctactaatca aaaggttatt gcagaaattc aagtaagaac tatttttgaa
      601 gaagggtatg gagaaattga tcatagacta agatattctc ataatgaaat tccagagata
      661 ttaaaatcta atcttttact ttttaataga attgtaggaa gtgcagatga aatggcttcc
      721 ttaataaata tgctaaataa taatttagat gataaagaca attattacga aactaaatta
      781 aaagaaaaag atgaagaaat aagaaaatta aaagaagaaa ttgaaaagtt aaaataattt
      841 aaatgagcta catttatgta tcttataaat aaaactcata aacatttgtt gaaattattc
      901 atgaatttta tttttaagat ttaatgtagc tcttaatttt tagaaaatat aatattaaca
      961 aaatattaac
//
LOCUS       DAEVGI010000330          966 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEVGI010000330 DAEVGI010000000
VERSION     DAEVGI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.332"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            138..>966
                     /locus_tag="K8M77_002621"
     CDS             138..>966
                     /locus_tag="K8M77_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBI7087040.1"
                     /translation="MNINAEFHCHSLASDGTLSPKEVIAEAKKSGISLIALTDHDITS
                     GLDEAKEAAKELDIQFIPGIELSCEHKGSTIHILGYFRDDSYKSEDFQKFLNELKDSR
                     IGRAKKIVANLEKYFNIKLDYKDVLAKGKGVVARPHIAQCIIDAGYPYDWEYIFDNFI
                     GNDSPAYVPNKKISVEEGINILKANNAITVLAHPKLIKRVPVKEVMEFPFDGIEAIYY
                     QNTKKDTDFFISYAVHHDLLITCGSDFHGDHEGDERHGHVGCMSIPEEYVEKFLKKYN
                     "
BASE COUNT          371 a          117 c          150 g          328 t
ORIGIN      
        1 aaagttaaaa attaatttat tttattctaa tttatatgtt aaaatattta tactacaaat
       61 tttttaaaat taacaatatt attcaaataa ttattataca tacatttatt tttttcagtt
      121 ttaaaggagg gtttactatt aatattaatg cagaatttca ttgtcactca ttagcttctg
      181 acggaacact atctccaaaa gaggtaattg ctgaagctaa aaaatcaggt atatctttaa
      241 tagctttaac agatcacgat ataacctctg gtcttgatga agcaaaggaa gccgcaaaag
      301 aattagatat tcaatttatt cctggaatag aacttagttg tgagcataaa ggatctacaa
      361 ttcatatact aggatatttt agagatgatt cttataaatc tgaagatttt caaaagttct
      421 taaatgaatt aaaggattct agaataggta gagcaaaaaa aatcgtagct aatttagaaa
      481 aatattttaa tattaaactt gattataaag atgtcttagc aaagggaaaa ggtgttgttg
      541 ctaggcctca tatagctcaa tgcataatag acgctggata tccttatgat tgggaatata
      601 tatttgataa cttcatagga aatgatagcc cagcttatgt ccctaacaaa aaaatttctg
      661 tagaagaagg aataaatata ttaaaagcta ataatgctat aacagtgctt gctcatccaa
      721 agcttataaa aagagttcca gtaaaggaag ttatggaatt tcctttcgat ggtatagaag
      781 ctatttacta tcaaaacact aaaaaagata ctgacttctt tatttcatat gctgttcatc
      841 atgatcttct tataacatgt ggttcagatt ttcatggaga tcatgaagga gatgaaagac
      901 atggtcatgt tggatgtatg agtataccag aagaatatgt tgaaaaattc ttaaaaaaat
      961 ataatt
//
LOCUS       DAEVGI010000331          951 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEVGI010000331 DAEVGI010000000
VERSION     DAEVGI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.333"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            147..773
                     /locus_tag="K8M77_002622"
     CDS             147..773
                     /locus_tag="K8M77_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HBI7087041.1"
                     /translation="MSKHYKPKEFAELLNVSVITLQRWDNDGKLKAFRTPTNRRYYTY
                     EQYLEYIGIHKETDSRKIVIYTRVSTSNQKDDLKNQVEFLRQYANAKGIIVDEIIEDY
                     GSGLNYNRKKWNKLIDSCMTNEISTIIITHKDRFIRFGYDWFESFLAKFNVKIIVVNN
                     ESLSPQKELVQDIISILDTFSCRIYGLRKYKKKIRDDEEVEKSIKDRN"
     gene            763..>951
                     /locus_tag="K8M77_002623"
     CDS             763..>951
                     /locus_tag="K8M77_002623"
                     /inference="COORDINATES: protein motif:HMM:NF023742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI7087042.1"
                     /translation="MEINPNYKKKSKIHRTIGVSIFIYNFYISHNKEVYEKDGKFLSD
                     MDFSKRLNNKYTPNNQDKN"
BASE COUNT          419 a           88 c          147 g          297 t
ORIGIN      
        1 taagaattat aaatctttct taaaatagta acatcaactt ataaaattaa gaaagttttt
       61 aataaaggag atgctcatta tttgataaag atataatcaa atatagtcta atatgatata
      121 atatagtcat aaaaggatgt gattatatga gtaaacatta taagcctaaa gaatttgcag
      181 aattattaaa tgtatcagtt attacactgc aaagatggga taatgacgga aaattaaagg
      241 catttagaac tcctacaaat agaagatact acacttatga acagtatctt gagtatatag
      301 gaatacataa agaaacagat agcagaaaaa tagttattta tacacgagtt tcaacttcta
      361 atcagaaaga tgacttgaaa aatcaagttg aatttcttag acaatacgct aatgctaaag
      421 gaataatagt agatgaaatc atagaggatt atggaagtgg actaaattat aatcgtaaaa
      481 aatggaataa acttattgat agttgtatga caaatgagat aagtacaatt atcataactc
      541 ataaggatag atttattcgg tttggatatg attggtttga aagtttttta gctaaattca
      601 atgtaaagat tatagttgta aataatgaaa gtctttcacc acaaaaagaa ttagtccaag
      661 atataatatc aatacttgat acttttagtt gtcgcatata tggattaaga aaatataaga
      721 aaaaaattag agatgatgaa gaagttgaaa agagcataaa agatagaaat taatcctaat
      781 tataagaaaa agtctaaaat acaccgaact attggtgtat caatatttat atataacttt
      841 tatatatctc ataataaaga agtttatgag aaagatggaa agtttttaag tgatatggat
      901 ttttcaaagc ggttaaataa taagtatacc ccaaataatc aagataaaaa t
//
LOCUS       DAEVGI010000332          938 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEVGI010000332 DAEVGI010000000
VERSION     DAEVGI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.334"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            65..739
                     /locus_tag="K8M77_002624"
     CDS             65..739
                     /locus_tag="K8M77_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HBI7087043.1"
                     /translation="MQYLIYSLIAVIILTVSLNNIFKKQSKYFYMLAILISVVVTSYE
                     IFEIWTGFKLEGIIFVLERSFMKGFVSTALFILVMFAGALSKKWGITKKLLRVRAEMA
                     ILASILILPHFIIYTYKFLVRLFSGKPLSILYISFIIVGLIAFIIMIPLFITSFKKVR
                     VTMSPSKWKMVQRWAYPFYFLVYLHIILILFNKKVFNLNAVILYTVIFLGYFILKICN
                     NKKNIK"
BASE COUNT          372 a           73 c          121 g          372 t
ORIGIN      
        1 taataattta tatagtaata tctaaatgaa aatgattgtc agtaacaagt ggaaggagaa
       61 atcaatgcaa tatttaattt attcattaat agcagtaata attttaacag taagtctaaa
      121 caacatattt aaaaagcaat ctaaatattt ttatatgctg gctattttaa tatcagtagt
      181 agttacatca tatgagattt ttgaaatatg gacaggcttt aaattagaag gaattatatt
      241 tgttttagaa agaagcttta tgaaaggctt tgtatcaaca gcattattta ttttagtaat
      301 gtttgcagga gctttaagta agaaatgggg aattactaaa aaacttttaa gagttagagc
      361 agaaatggca attttagcat ctatacttat attacctcat tttataatct atacttataa
      421 gttcttagta agattattta gtggaaagcc attaagcatt ttatacatat cttttataat
      481 agtaggatta atagcattta taataatgat acctctattt ataacatcat ttaagaaagt
      541 aagagttact atgtcaccaa gtaaatggaa aatggttcaa agatgggcat atccttttta
      601 ttttctagtg tatttacata taatattaat cttatttaat aagaaagttt ttaatttaaa
      661 tgctgtaata ttatatacag ttatattctt aggatatttt attttaaaaa tatgtaataa
      721 caaaaaaaat attaaataat ataaaagtta gtatttttaa aaacttcatt tatagtaaaa
      781 atttaattat tactatagat gaagtttttt tattatatag ttaaaagata aaatagtata
      841 agaaaaaaat aagttgtttt tatattatag taagagttga cttttacatt tttaagatta
      901 agcttgattt ttaatattta agagtttgtt tttacaat
//
LOCUS       DAEVGI010000333          935 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEVGI010000333 DAEVGI010000000
VERSION     DAEVGI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.335"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            308..859
                     /locus_tag="K8M77_002625"
     CDS             308..859
                     /locus_tag="K8M77_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087044.1"
                     /translation="MLIILYLSFFLIITISIFLGRGKSLVKQKLFLTLSSFLILIGII
                     TSFLIKSIFLTNLRIHNELYDYVSLEFINWALNKFNSYFKWSYLYVLIVLGVLLYNLY
                     TDHNIRNRESLKHFNYLCVTSMVVILTGAIIYSFSSINKVFDIPLYLEITAFSQIFIL
                     YIPLVAMRLYIGNPEVQDTIFEV"
BASE COUNT          334 a          116 c          100 g          385 t
ORIGIN      
        1 aattataaat taccttttga ttcctctttg aaccttggta cttcttaaag caccttactc
       61 accattgaga tttttacctg ttttaaattt gctttctgct gttatttttg aataatccta
      121 gcttaaatta ctcaaaaata aaagatagat actcagtttg aataactgtc taggaaaatc
      181 tctacaaatt aattgattaa ttattagatt ttataaaaaa atatttaata acaaacatta
      241 acttccatta tttgtaattt attaaggttt aagctataat taatatgtaa acttaaacga
      301 gttggaggtg cttattattc tgtatttatc tttctttcta ataattacaa taagtatttt
      361 cttaggtaga ggaaaaagct tagtaaaaca aaaattattt ttaactttat cctctttctt
      421 aatattaatt ggaataatta cttcattttt aataaaaagt atatttttaa ctaacttaag
      481 aatccataat gaactttatg attatgtaag tcttgagttt ataaattggg cccttaataa
      541 atttaattct tactttaaat ggtcttattt atatgttctt atagttttag gagttctttt
      601 atataatcta tatacagatc ataatataag aaatagagaa agcttaaaac actttaacta
      661 tctttgtgta acatctatgg tggttatatt aaccggtgca ataatctatt cctttagctc
      721 cataaataaa gtctttgaca taccattata tttagagata acagcttttt ctcaaatatt
      781 tatactttat attccactag tagcaatgag actttatata ggaaatccag aggttcaaga
      841 tacgatattt gaagtataat aacattctta tatttttcgt acatctaata agagtatatt
      901 atttatatat ttaagcttaa taattatata actct
//
LOCUS       DAEVGI010000334          929 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEVGI010000334 DAEVGI010000000
VERSION     DAEVGI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.336"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            85..321
                     /locus_tag="K8M77_002626"
     CDS             85..321
                     /locus_tag="K8M77_002626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087045.1"
                     /translation="MGDFIKNYILGAIQVDFIDEDYEDVELRCKFNLEKKIDDLLDKY
                     EYFKKEIQCTHDKVHRQIFYYKLEKLNDKLILKK"
BASE COUNT          424 a           63 c          131 g          311 t
ORIGIN      
        1 taaatgtttt tcgcaaactc attgtattct caaagattga aaagagcaat gttcatttaa
       61 cttaacagat cgaaagaaat agatgtggga gattttataa aaaattatat tttaggagca
      121 attcaagttg attttataga tgaggattat gaagatgttg aattaaggtg taaatttaat
      181 ttagagaaaa aaatagatga tttattggat aaatatgagt actttaaaaa agaaattcaa
      241 tgtacgcatg ataaagtaca tagacaaata ttttattaca agttagaaaa attaaatgat
      301 aaattaattt taaaaaaata ggacaagatc catctttttt agaagtaaat ttttatcagt
      361 atgataataa atataaaaaa ttaaaatttt atgaagagat taaagatgac tttaaaaatt
      421 caatacattc atattatgag ggtctaatag ttggagcttt tacatattta agaagagttt
      481 tagaaaagat acttgaatat aaatttaatg atattcgtaa tgaattagaa atagatgaag
      541 tagaaaattt tgaaaaagaa actaagtttg ctaaaaaatt aaaaatatta aaaagatatt
      601 ttccagaata tttaacagca aatactcaaa tatactcagt tttaagtaaa ggcgttcata
      661 aattatcaga agaagaagct aatgaatatt ttgaagttgt aagaagttgt gtttatatta
      721 tacttaatga ctttttaaat gaacaagaag ataaaaaatt aaaaatggat ataagtaaga
      781 aaatcaatga tattaattct gaaataaaaa ataattaaaa aactcactta aagaagagac
      841 ggagaaccct atcacaaata atgaagataa tttagatttt taaaagtttt agattatata
      901 aaactttttt gtaaaaatat tgtaattaa
//
LOCUS       DAEVGI010000335          925 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.337, whole genome shotgun sequence.
ACCESSION   DAEVGI010000335 DAEVGI010000000
VERSION     DAEVGI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.337"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(16..645)
                     /locus_tag="K8M77_002627"
     CDS             complement(16..645)
                     /locus_tag="K8M77_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBI7087046.1"
                     /translation="MKDIVIIGTENIGKEVVKIIEAINSKRNTWNVLGFISLKKDEAL
                     SNIEGYSVLGSIDSMFNYFEGRKKDKFYFFKKLESQNELFTIIAIDNCKLKKEIEDKL
                     KDKIKFATIIHPDITFFNKQEVGEGTIIYPGLISVGRFKLGKHVILKQKCSLGNNIEI
                     GDYSFISFNSNIDSNVMVKEGAYIEANTTILANNNIDKNTYIKEGSILY"
BASE COUNT          327 a          142 c           54 g          402 t
ORIGIN      
        1 aatataatta taattttaat ataatattga accttctttt atataagtat ttttatctat
       61 attattattt gccaatattg tagtattagc ttcaatataa gctccctctt taaccattac
      121 attgctatca atattactat taaaagatat aaaagaataa tctcctatct caatattatt
      181 tcctaaagaa catttttgtt ttaaaataac atgtttacct agcttaaatc ttcctacact
      241 aattaatcct ggatatatta tagttccctc gccaacttct tgtttattaa aaaaagtaat
      301 atcaggatga ataattgtag caaattttat tttgtctttt aacttatctt ctatttcttt
      361 ttttaattta cagttatcta ttgctataat agtaaataat tcattttggc tttctaattt
      421 cttaaaaaaa taaaacttat ctttttttct accttcaaaa taattaaaca ttgaatctat
      481 agatccaaga acactatatc cctcaatatt acttagtgct tcatcttttt taagacttat
      541 aaatcctaat acattccaag tatttctttt tgaatttata gcctctatta ttttaacaac
      601 ctcttttcct atgttttcag ttccaattat aacaatatcc ttcattaaaa cacctcacta
      661 aattaatttt ttaaagatct atatcaaaac gctcttattt attaataata taatatcaat
      721 tcaaaatatt aattaactta ttaaatcata ttaatttaga tatttattta ttctcaatta
      781 ttttttacaa tttatgtata ttaatccgat atttgtccat aattaaaata tccacgatta
      841 ataagtagat taattcttag ttttaaaata attaatgata cttccccaac aatatatcct
      901 taataacttc cccttttatt gctat
//
LOCUS       DAEVGI010000336          906 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEVGI010000336 DAEVGI010000000
VERSION     DAEVGI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.338"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..691)
                     /locus_tag="K8M77_002628"
                     /pseudo
     CDS             complement(1..691)
                     /locus_tag="K8M77_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466614.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT          351 a          162 c           81 g          312 t
ORIGIN      
        1 taaagattct attaaatttt gcccctataa aacctcctaa aacacctcca aatatcatat
       61 atggaagcat ggatagatca tacccagaaa agccagttcc aaaagcaata gttaaaagtt
      121 tagatgcttg agaaaatagt attataagga cagaatctct agcaacattc ttagcttcca
      181 tggaaaagaa tattgttaaa aaggcaacat ttattggtcc tcccccaata cctagaaagg
      241 ctgctattaa tactaaaaat atcccaatta tagtgcttaa aataaagtta tcaattttgt
      301 aaccttaaac ttttctttat tattcatgta tatataaaca aatattaata acaatgctaa
      361 aattccactt tgcactaaag atacgattct tccttgaaca atttttaata taaaagttaa
      421 taacttttgt cctaataatc ctccaattac agaaccaata cctaataatc cagtattttt
      481 tatatcaatc ttacttttat aagataattg tttgattacc gaaactaagg ccatagaaaa
      541 aaatagtaca tgctgataaa actccaatag tttctatatt gtagtctcct aaaaaatcta
      601 aaactggctt tattattact cctccaccaa gcccatcaat tgatccacaa attgttgata
      661 atagagccac taaaaaatat attaaaacca tattaccact tccttaaatt agctatttat
      721 attattttaa aattctcatc tattatattc ttttacattc tctcctttcc atatttaatt
      781 taaaaagctc gcctaaactc acctaaaaac tctatattta tatattacaa caaagtattt
      841 caaaaagctt aaaccctaaa ttgtttacat aagaaatctt atgtagtatg ttatacttat
      901 atatta
//
LOCUS       DAEVGI010000337          887 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.339, whole genome shotgun sequence.
ACCESSION   DAEVGI010000337 DAEVGI010000000
VERSION     DAEVGI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.339"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            88..834
                     /locus_tag="K8M77_002629"
     CDS             88..834
                     /locus_tag="K8M77_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087047.1"
                     /translation="MDVRIYKINTGITGRNFNSDKRSNFPIGGKTNSFYSIGNLMRGI
                     EDFYLEGNLYFIDKEEELEKFYNPLGDRWVAKKKYYISHPKKERENYLLEASKFHEYI
                     VREQLLEIANYLHHELNINYLKIEVEDGNDFSFYTNVALEEMNLNDEGKVELKGDKTL
                     EIKWSENINSQNEFLWIDDFEGLVNRVQELKASNKRGEKEGLEGNSLGERGISYILEI
                     DNSFGISEKIGKKIGVNHAWLKKCSFKLVF"
BASE COUNT          345 a           70 c          192 g          280 t
ORIGIN      
        1 tggtatattt aaagaaagcg ttttaattac atgaaaaggt aatcatttta aacttaaaag
       61 ataaaacatt aaggtgagga ttagagaatg gatgttagaa tatacaaaat taatacagga
      121 ataacaggaa gaaattttaa tagtgataag aggagcaatt ttcctatagg aggaaaaact
      181 aattcttttt attcaattgg taacttaatg agaggaatag aggattttta tttagaagga
      241 aatctttatt ttatagataa ggaagaagaa cttgagaagt tttataatcc tttgggggat
      301 agatgggtgg caaagaagaa atattacata agtcatccta agaaagagag agaaaattat
      361 cttttagagg ctagtaagtt tcatgagtat atagttaggg agcaactttt ggagatagca
      421 aattatctgc atcatgagct taatataaat tatttaaaga tagaggttga agatggaaat
      481 gacttttcat tctatactaa tgtagccctt gaagaaatga atcttaatga tgagggaaaa
      541 gtagaactaa agggagataa gactttagag attaaatgga gtgaaaatat aaattcacaa
      601 aatgaatttc tgtggataga tgattttgaa ggcttagtta atagggttca ggaacttaag
      661 gcttctaata aaagaggaga gaaggaaggt ttagagggaa attctttagg tgaaagagga
      721 atttcttata tcttagagat agataattcc tttggaatta gtgaaaaaat aggaaagaag
      781 ataggggtta atcatgcttg gcttaagaaa tgctctttta aactggtatt ttagaagtct
      841 gatttaataa acttgtccaa tagtttatgt ttttatgcct ttggcaa
//
LOCUS       DAEVGI010000338          874 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEVGI010000338 DAEVGI010000000
VERSION     DAEVGI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.340"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          336 a           70 c           92 g          376 t
ORIGIN      
        1 ctaaaagctg attggctaac tttatttttt gtgtaagcaa attatggagg gattaaatgt
       61 ttagtctatt aacttttgag tttacaaatt atgttaattg caaaatatat aacttaataa
      121 gctctaataa aatgttaaat agcaaaaata tatttaggta tatttttgca ttatatttag
      181 tcgtattatc gacaatattt ttaataagta cctttatatt tgagttctta agaatatcaa
      241 atgcagattt aattcagtat attaacatca ttaaagtaat agttattata tatattttta
      301 ttcttccttt tacaaaaata tcaaaaatat ataatcaatt aaatagagat gaactttata
      361 gtagtttttt attcaataca aatatatcct ttaagggata tatattcatt agagttttaa
      421 ttcattctat ttttctatta actttaactg gtttatcagt aattgcaata ctttttagta
      481 tacaaatttc attaggatat ggggtaggat attcattatc atttttaata aaatatttta
      541 tttattgtat ttatttcttg caattaagaa tgatattgat atcatataga attattgctg
      601 gtaagtataa tgccatattt aatttttgta taatttgcat atcattaata ttaataattt
      661 ttaaaaatat taatattcag ttgctcaagt tatctataaa taacttgata gctttaataa
      721 gttcgaatta tttattagtt ttattagtgc ttactgtctt taattttatt ttattaaata
      781 aatcagaagt aagtttaaaa caaaaagttt tagaatataa aaataaaaat aaattagcaa
      841 aaaaaattta tttattaaaa gaaagaaata gttt
//
LOCUS       DAEVGI010000339          839 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEVGI010000339 DAEVGI010000000
VERSION     DAEVGI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.341"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            32..>839
                     /locus_tag="K8M77_002630"
     CDS             32..>839
                     /locus_tag="K8M77_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7087048.1"
                     /translation="MYDSQKEERKLKRKLFFKFFNYNFLKDRNIEYKEFKYGDNKRDY
                     YRVYKGYDKRKPTIFFIYSGGLWNSSPKGCAAIGKFFYKYGFTVVIPSYELAPEHKYP
                     VQMENIFSAFKHYLENNKPNRIVVIGYSSGADLAANLIYNKDMKEKFHIDTGIISSFI
                     SLGGTLDFSQCNSKEYKKYIKKYLYGKEQIFNVNPINLLKEDSPKIPVLCVHGAKDSI
                     VSYENSKRFVEKVNKFKGNGEFLKLEGYQHLDLLNLFIGLGNKKTGDIMSF"
BASE COUNT          351 a           77 c          137 g          274 t
ORIGIN      
        1 aatataattt taaactttaa agagattata aatgtatgat agtcagaaag aagagagaaa
       61 attaaagaga aaattatttt ttaaattctt taactataat tttttaaagg atagaaatat
      121 agaatataaa gaatttaaat atggtgacaa taaaagagat tactatagag tatataaagg
      181 ctatgataaa agaaagccta ctatattttt tatatatagt ggaggtttat ggaatagcag
      241 ccctaaagga tgtgcagcta taggcaagtt tttttataaa tatggattta ctgtagtaat
      301 tccatcctat gagctagcgc cagaacataa atatccagtt caaatggaaa atatattttc
      361 tgcatttaag cactatttag aaaataataa acctaataga atagtagtta tcgggtattc
      421 ttcaggggca gatttagctg caaacctgat ttacaataaa gacatgaaag aaaagtttca
      481 tatagataca ggcataatat cttcttttat atcactagga ggaaccttag attttagtca
      541 atgtaattct aaagaatata aaaaatatat aaaaaaatat ttatacggaa aagaacagat
      601 ttttaatgtt aatccaataa atttactaaa ggaggattca cctaaaattc cagtactttg
      661 cgttcatggt gctaaggatt ctatagttag ctatgaaaat tctaaaaggt ttgtagaaaa
      721 ggtaaataaa tttaaaggaa atggagaatt tttaaaatta gagggatatc aacatttaga
      781 tttgttaaat ttatttatag gattaggaaa taaaaaaaca ggggatataa tgagtttta
//
LOCUS       DAEVGI010000340          832 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEVGI010000340 DAEVGI010000000
VERSION     DAEVGI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.342"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(234..>832)
                     /locus_tag="K8M77_002631"
     CDS             complement(234..>832)
                     /locus_tag="K8M77_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HBI7087049.1"
                     /translation="NGILISKFLEDSKVNMSTFTSLEFLEKLSINLRKLHILKCEHIF
                     NPFEHIRKNFHILKSKNFNFHQDIDLVLNKLNILEEKLSKNMTIGLCHNDLNSSNVLY
                     YNKNVLFIDFEFSAMCDIFFDLATVSWMLDEKKRYFLIKSYFGYYSYELMEKLENYLF
                     VVKLWNASWSFLKSLNTNSTYDYKLGGNMIIDDLLSTL"
BASE COUNT          318 a          101 c           87 g          326 t
ORIGIN      
        1 tataaaactt acaaacaaat tctataattg ctagaatttc agtgaaaggc caaattttta
       61 atttggattc ttaaaattat gtaatggaac tttgtaaaat tatattccta agaaattaat
      121 agtcttatct ttttaacgtg atattttcta cgtaataatt taaattctaa agtttatcta
      181 gatattctct tcacactgtt tgtaagtttt attttatatt ttgatgcact cttttaaaga
      241 gtggataata aatcatctat gatcatattt ccacctaatt tataatcata tgtagaatta
      301 gtatttaaag acttcaagaa gctccaagaa gcattccaaa gcttaactac gaaaagataa
      361 ttttctaatt tttccattaa ttcataagag tagtagccaa aataactttt tattaaaaaa
      421 tacctcttct tttcatcaag catccaagat acagtagcta aatcaaaaaa tatatcacac
      481 atagcagaaa attcaaaatc gataaaaaga acatttttat tataatatag tacatttgaa
      541 gaatttaaat cattatgaca aaggccaata gtcatatttt tgcttaattt ttcttcaaga
      601 atatttaatt tatttaagac taaatcaata tcttgatgaa aattaaagtt tttagatttt
      661 aaaatatgaa aattttttct tatatgttca aaagggttaa aaatatgttc acacttaagt
      721 atatgtagtt ttcttaaatt aattgagagt ttttcgagga attctaaaga tgtaaatgta
      781 ctcatattta cctttgaatc ttctaaaaat ttgcttatta gtattccatt at
//
LOCUS       DAEVGI010000341          823 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEVGI010000341 DAEVGI010000000
VERSION     DAEVGI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.343"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(114..740)
                     /locus_tag="K8M77_002632"
     CDS             complement(114..740)
                     /locus_tag="K8M77_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI7087050.1"
                     /translation="MSNKIIKSYWGVELSPRFIDARSKKLCIILPGIGYCLDRSYLDY
                     SKQLAKEIGYDVLEIEYGYQINRSEFNIEKEFSIMVSESLEVIDNAIEKEYEEILIIG
                     KSIGTSVQSFLNKDLSGRFKSIRNIYISPINKTLDEFNIESNSLVITGDKDPLLSIAH
                     RVEIEKREDIESFIIEGANHALDIEGNVMKTVDALKESLEIEKDFILR"
BASE COUNT          274 a          139 c           63 g          347 t
ORIGIN      
        1 ttattaaacc ctttacattt aatacatgga actagtttca taaaatattt aaaataaaaa
       61 agaataaggt taatcatgaa tggttaacct tattcttttt cattttaatt tgattatctc
      121 agtataaagt ctttttctat ttctaaagac tcttttaaag catcaacggt tttcataaca
      181 tttccctcta tatcaagagc atgatttgca ccttctatta taaaactttc tatatcttct
      241 cttttttcta tctctactct atgagctata cttaataatg gatctttatc tcctgttata
      301 actaaagaat tagattctat gttaaactca tctaaagtct tatttattgg gcttatatat
      361 atatttctta tacttttaaa tcttccactt aaatctttat ttaaaaaaga ctgtacactt
      421 gttccaatac tttttcctat tattaaaatc tcttcatatt ctttttctat ggcattgtct
      481 ataacttcta atgactcaga aaccattatg ctaaactctt tttcaatatt aaattcgctt
      541 ctatttattt gatatccata ttctatttct agaacatcat atcctatttc ttttgctaat
      601 tgcttagaat aatctaagta agatctatct aaacaatatc ctattccagg taatattata
      661 caaagctttt tacttctagc atcaataaac cttggactta attcaacacc ccaatatgat
      721 tttattattt tattactcat tttaaaaacc tcctaatttt taaaattttc aaaaatctat
      781 cctaaaaagt gatatgaaag ggagaaaaaa tatttcactt ctt
//
LOCUS       DAEVGI010000342          819 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEVGI010000342 DAEVGI010000000
VERSION     DAEVGI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.344"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(25..579)
                     /locus_tag="K8M77_002633"
     CDS             complement(25..579)
                     /locus_tag="K8M77_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBI7087051.1"
                     /translation="MEMKTRDLTLIPIFAALTAIGAFIKIPIPIVPFTLQYFFCALGA
                     ILLGAKRGALSQIIYVLVGLLGIPVFTQGGGPSYVFQPTFGYLIGFIFGAYIIGKITE
                     NLKSINIKNLFLAGISGLLVIYLFGVSYMYVIYNFYLGDAMSLLSAVSIGAIACLPSD
                     LLLTFIISLVGSKVCPRLKIFDYI"
BASE COUNT          320 a          135 c           89 g          275 t
ORIGIN      
        1 actcataaaa atcaactccg ctctttaaat ataatcaaat atttttaacc ttggacaaac
       61 cttagatcct actaatgaaa ttataaatgt caataataaa tcacttggta aacatgctat
      121 ggctccaata ctcactgctg ataataagct cattgcatcc cctagataaa aattataaat
      181 tacatacata tatgaaactc caaataaata gattactaaa agtcctgaaa ttccagctaa
      241 aaataaattt ttaatattta tactttttag attttctgtt atttttccaa ttatataagc
      301 tccaaaaata aaaccaatta aatatccaaa ggtaggttga aatacatatg atggtcctcc
      361 tccttgagta aatacaggaa ttccaagtag tcctactaat acatagatta tttgagataa
      421 agctcctctt ttagcaccaa gtaaaattgc tcctaaggca caaaagaaat attgaagtgt
      481 aaaaggcact attggtattg gtatcttaat gaaagcccca atagctgtaa gcgctgcaaa
      541 aataggtatc agtgttaaat ctcttgtttt catttccata gttaaatctc ctttaatctt
      601 atacatccat acaattttaa aaatcaattt atcttgattg tcaaattata ttccatataa
      661 taaccttata atccacaatt gtcaactatt ttatattttt aggtttacaa taaaacttaa
      721 ttagtttacc ttaaacccta tttaaaaata aaaggggctg tatcaaaata taataaaact
      781 tacaaacagt atgaagagaa tatctagatg agctttaga
//
LOCUS       DAEVGI010000343          811 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEVGI010000343 DAEVGI010000000
VERSION     DAEVGI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.345"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..360
                     /locus_tag="K8M77_002634"
     CDS             <1..360
                     /locus_tag="K8M77_002634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087052.1"
                     /translation="LEEKTIKFTNTKGGRELTRDIPDDRINLVKEVLEKNYHEDRLLS
                     IQGDSINRYLNRIEDKLGIERHSNHDIRRLIAQEKYDSYRESGLSIKDAANKTSTWLS
                     HGDNRNELLEKSYIKLK"
     gene            633..>811
                     /locus_tag="K8M77_002635"
     CDS             633..>811
                     /locus_tag="K8M77_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087053.1"
                     /translation="MFNLYNEFLKYEYNELQELFKMARSKEEQDFYMALCNFKLQKEQ
                     KKVIGNDFEMELTLN"
BASE COUNT          353 a           57 c          133 g          268 t
ORIGIN      
        1 ttagaggaga aaactataaa atttactaat acaaaagggg gaagagaact tacaagagat
       61 attccagatg atagaataaa tcttgttaag gaagtactag aaaaaaatta ccatgaagat
      121 aggctgctga gtatacaagg agattctata aacagatatt taaatagaat tgaagataaa
      181 ttaggtatag aaagacacag taatcacgat attaggcgtt taatagcaca ggagaagtat
      241 gatagttata gagaatcagg attgagtata aaagatgcag ctaataaaac aagtacttgg
      301 ttaagtcatg gagataatag aaatgagctg cttgaaaaga gctatataaa attaaaataa
      361 aattttagat ttataaatat taacttgaaa tttatttatt gattaaaaga tgagtttaat
      421 ttaaaattta gattaatact gtaattttgt ttactaaaaa ttaaagaact agtatttact
      481 tttatatttt taaattgcaa aaattaattg tcaaaatact aaaaagaaga tgaaatttta
      541 tatttatttc tgaagttcct tattttgagg acattgaaaa aatataggtt ttatcttttt
      601 ttatattaat taaagttagg gagttaaata ttatgtttaa tttatataat gaatttttaa
      661 aatatgaata caatgaatta caagagctgt ttaaaatggc tagaagtaaa gaagaacagg
      721 acttctatat ggcattatgt aactttaaat tacagaaaga acaaaagaaa gttattggaa
      781 atgattttga aatggagtta acattaaatg a
//
LOCUS       DAEVGI010000344          808 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEVGI010000344 DAEVGI010000000
VERSION     DAEVGI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.346"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            94..786
                     /locus_tag="K8M77_002636"
     CDS             94..786
                     /locus_tag="K8M77_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBI7087054.1"
                     /translation="MKYEVILFDADETLFDFKKSEKEAFKNAMLDFNIAYDENYHLKV
                     YKEINTAIWKEFEQGLITQKKLKIERFKRLSDKLNINFDAANFAKSYMNHLADASFLY
                     EDSTDLVENLNKYYKLSIITNGLSSVQDKRIRQSTIAKYFDPIVISEEILISKPDPKI
                     FEHTLKHMNFSDKSKVLMVGDSLTSDIQGGINFGIDTCWYNPNKIKNETSIKPTYEIS
                     SFDELKSLLINS"
BASE COUNT          347 a           91 c          106 g          264 t
ORIGIN      
        1 atataaaata tcataaaaag ataaataaaa aatctcttat taaattaata atgaaactat
       61 gtatataaaa atacaaaagc tggaggatat attatgaaat atgaagttat actatttgat
      121 gcagatgaga ccttatttga ttttaaaaaa tctgaaaagg aagcctttaa aaatgctatg
      181 cttgacttta atatagcata tgacgaaaac taccatttaa aagtatataa agaaataaat
      241 actgctatat ggaaagagtt tgagcaagga cttattacac aaaagaaatt aaaaatagaa
      301 agatttaaaa gattatcaga taaattaaat ataaattttg atgcagctaa ctttgctaaa
      361 tcatatatga atcatttagc tgatgcttct tttctatatg aagatagtac tgaccttgta
      421 gaaaatctta ataaatatta taagctttct ataataacta atggtcttag ttctgttcaa
      481 gataaaagaa taagacaatc tactatagca aaatattttg accctatagt aatttctgaa
      541 gaaatactaa tctcaaaacc tgatccaaaa atctttgagc atactctaaa acatatgaat
      601 ttttctgata agagtaaagt attaatggtt ggagatagtt taacttctga tatacaaggt
      661 ggtataaact ttggtataga tacttgttgg tacaacccta acaagattaa aaatgaaact
      721 tcaataaaac ctacttatga aatttctagc tttgatgaac taaaatcttt actaattaat
      781 tcataaataa taataaaaag ggcgtgta
//
LOCUS       DAEVGI010000345          808 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEVGI010000345 DAEVGI010000000
VERSION     DAEVGI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.347"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            64..>808
                     /gene="xth"
                     /locus_tag="K8M77_002637"
     CDS             64..>808
                     /gene="xth"
                     /locus_tag="K8M77_002637"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBI7087055.1"
                     /translation="MKLISWNVNGLRACVKKGFLDYFKSEDADIFCLQETKLQEGQID
                     LDLEGYHQYWNYAEKKGYSGTAIFTKKEPLNVYYGINMEHHDKEGRVITLEFEDFFMV
                     TVYTPNSQSELARLEYRMEWEDDFRNYLLDLSSKKGVVVCGDLNVAHKEIDLKNPKTN
                     RKNAGFTDEERDKFSTLLSSGFIDTFRYFNPDLEGIYSWWSYRFNARKNNAGWRIDYF
                     LVSNNLEDRIKEASIDTEILGSDHCPVKLI"
BASE COUNT          303 a           86 c          154 g          265 t
ORIGIN      
        1 tcagttgtta tattttaaaa cttaattata ttttttattg attaagtata ggaggaaatt
       61 taaatgaaat taatatcatg gaatgttaat ggcttaagag cttgtgttaa aaaaggcttt
      121 ttagattact ttaaatctga agatgctgat atattctgtc tacaggagac taaacttcag
      181 gaaggacaaa tagatttaga tctagaggga tatcatcaat actggaacta tgctgagaaa
      241 aaggggtatt caggaactgc aatattcact aaaaaagaac ctctaaatgt ttattatgga
      301 ataaatatgg agcatcatga taaagaagga agagttataa ctttagaatt tgaagacttc
      361 tttatggtaa ctgtttatac tccaaactcc caaagtgaac ttgctaggtt agagtataga
      421 atggaatggg aagatgattt tagaaattat cttttagatc tttcatctaa aaagggagta
      481 gttgtatgtg gagatttaaa tgtagctcat aaggagatag atttaaagaa tcctaagaca
      541 aatagaaaaa atgctggctt tacagatgag gaaagagata aattttctac attattatca
      601 agtgggttta tagatacttt ccgttatttc aacccagact tagaggggat ttattcttgg
      661 tggtcatata gatttaatgc aagaaaaaac aatgctggat ggagaattga ctatttctta
      721 gtttcaaata atttagaaga tagaataaaa gaagcttcta tagatactga aatactagga
      781 tctgatcact gtcctgttaa actaataa
//
LOCUS       DAEVGI010000346          806 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEVGI010000346 DAEVGI010000000
VERSION     DAEVGI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.348"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            182..652
                     /locus_tag="K8M77_002638"
     CDS             182..652
                     /locus_tag="K8M77_002638"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBI7087056.1"
                     /translation="MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAI
                     MDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFVGELLNKNNYKISNIDATIIAQR
                     PKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLIK"
BASE COUNT          332 a           93 c          123 g          258 t
ORIGIN      
        1 atatatcttt tacataatta aaacatataa agattttaat cttataaagc tatatttttg
       61 ttacaattaa gtttaatttt acttaatagt tattgatact ttgataacat aacagaatta
      121 tatataatta aaccataaaa ataaattttc attgaaaaaa taaatctaag aggtgataaa
      181 aatgagaata ggaatgggat atgatgttca taaattagtt gaaaatagag atttaatatt
      241 aggtggagta aaaattcctt atgaaaaagg acttttaggc cattcagatg cagatgtatt
      301 attacacgca ataatggatt cacttttagg agcagcggct ctaggagata taggaaagca
      361 ttttccagat acggacccta aatacaaagg tgctgatagc ataaaacttc tagaatttgt
      421 aggtgaactt ttaaataaaa ataattacaa aatatcaaat atagatgcaa ctataatagc
      481 tcaaagacca aaaatggctc ctcatatacc tactatgaga gaaaatatag ctaaagcatt
      541 aaatatagac ttagatcaaa taaatgttaa agcaactact gaggaaggac ttggttttac
      601 tggttctgga gaaggaatct catctcaaag tatttgtctt ttaataaagt aaaaaggagc
      661 tgtatcaaaa tataataata aaacttataa acatattacg ataattacta gattttaagt
      721 gaaagtccaa attttgaatt tggagtcttg aacttatgca atcgaacttt gtgagattgt
      781 attcctaaga agctaacaat atcagc
//
LOCUS       DAEVGI010000347          805 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEVGI010000347 DAEVGI010000000
VERSION     DAEVGI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.349"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            29..778
                     /locus_tag="K8M77_002639"
     CDS             29..778
                     /locus_tag="K8M77_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI7087057.1"
                     /translation="MKLTTKNTKNPKKLLYKSVYDKLYKMIIDGEYPLNTKLPSEPEL
                     AKSFGVSRMTLRQALALLQDDGLVKNIRGKGNFIISPEKHTKIDFLELIGNPIYKCHT
                     EKIKDIEFVFRLELDSDYTKQVLEKDSTAVVACERYYKSKNKVLAYAFTFISVETIKE
                     FKIDLNNESELLDFLEHNSYEISKSSVLEIKHSSAGNSLNPKYKLVGGNQCDLILETL
                     YDNDYSPPILFNKYYIPSQYSTFKIKARKKE"
BASE COUNT          326 a          104 c          109 g          266 t
ORIGIN      
        1 aaattttata aataagtaag gtgttaatat gaaactcact acaaaaaata ctaaaaaccc
       61 caaaaaattg ctttataaat ctgtatatga taagttatat aaaatgatta ttgatggtga
      121 atacccttta aatacaaaat tgccatctga gcctgagctt gcaaaaagtt ttggggttag
      181 ccgaatgact ttgcgtcaag cacttgcttt actacaagat gacggtttag taaaaaatat
      241 aagaggtaaa ggaaatttta taatatctcc agaaaaacat actaaaatag attttttaga
      301 gttaatagga aatcctatat acaagtgtca tactgaaaaa attaaagata ttgaatttgt
      361 attccgctta gaacttgata gtgattatac taaacaggtt ttagaaaagg attccacagc
      421 agtggttgct tgtgaacgtt attataaaag taaaaataaa gttttagcct acgcttttac
      481 ctttatatct gttgaaacta taaaagaatt taaaatagat ttaaataatg aaagtgagct
      541 actagacttt cttgaacata actcctatga aatatcaaaa agttctgtat tagaaataaa
      601 acattcctct gctggtaact ccttaaatcc aaaatataag cttgttggag gaaatcaatg
      661 cgatttaatt ttagaaacat tatatgataa tgattattcc cctcctatat tatttaacaa
      721 atactatatt ccatctcaat atagtacttt taaaattaaa gcaagaaaaa aagaataatt
      781 tgaactttta aaaatatttc tacat
//
LOCUS       DAEVGI010000348          786 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEVGI010000348 DAEVGI010000000
VERSION     DAEVGI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.350"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(141..488)
                     /locus_tag="K8M77_002640"
     CDS             complement(141..488)
                     /locus_tag="K8M77_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087058.1"
                     /translation="MFKENLKNLLTKYDTVELVLYDDKLNLYVMILCEECQCDVIREL
                     GIRGNEVENLKEIVNSLGYDFISLKEYYEKGTYDEYGNKMTCCCNNSSYKIVRVNFDK
                     DNEEDYYMAWATV"
BASE COUNT          294 a          125 c           42 g          325 t
ORIGIN      
        1 gtttaaattt ttttactttt taatgttatt ttaaagaata aaatatttaa agtaaaaatt
       61 taatactcaa ttaatatctt aacatacttt cctaaaataa attaaataaa aaataaaaag
      121 ccactaattt aagcagcttt ttagacggtt gcccatgcca tgtaatagtc ttcttcatta
      181 tctttatcaa aattcaccct aacaatctta taagaactat tattacagca acaagtcatt
      241 ttattaccat attcatcata ggttcctttt tcataatatt cttttaaaga aataaaatca
      301 taacctaatg aatttactat ctcttttaaa ttttctactt catttcctct tatgcctagt
      361 tctcttatta catcgcattg acactcttca catagtatca taacatataa attaagctta
      421 tcatcgtaaa gaactaattc tacagtatca tatttagtta ataaattttt gaggttttct
      481 ttaaacataa aattctcccc cttaagtata aacatatcat tacatttata tcctataagt
      541 aaaaatttca tacattaatt aaattatgta tcttaaaatt tattatactc ctctatattt
      601 tctcaatcaa acctacatag aaagcttaaa tcagtaaaat taatcaatat tcttattcaa
      661 actaatattt gtttactttt atactaaaaa tacaactttt cttttaataa ctcttttatt
      721 tgcttttaaa ttaaatattt aaaatattta tttaatttta taccctttaa aaaatcccac
      781 tttcct
//
LOCUS       DAEVGI010000349          778 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEVGI010000349 DAEVGI010000000
VERSION     DAEVGI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.351"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..708
                     /locus_tag="K8M77_002641"
     CDS             <1..708
                     /locus_tag="K8M77_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI7087059.1"
                     /translation="VDPKTNGVKGFESLLRLNKNGKILTPDSFIREIEDNNMSLEVSL
                     WLLKKVIADYRIIKDYRMVKGSNFYISLNVSFKEIENPKFLRSLMKIAKDYKIDDCNI
                     CLEIVEKFGMEDIGRIQSAIRIIKEYGFLIAIDDFGVEYSNLDLLNKIDSDIVKLDKY
                     FADNLDKSIINKKTVEFISEICNISNRTIVFEGVEGKYQVNIIKAFPYEKIYIQGYFY
                     SKPVDIETLKDFEVKDS"
BASE COUNT          321 a           54 c          145 g          258 t
ORIGIN      
        1 gtagatccaa agacaaatgg agtaaagggc tttgaatctc ttttgagatt aaataagaat
       61 ggaaaaattt taacacctga tagctttata agggaaattg aagacaataa tatgtcttta
      121 gaggtttctt tatggctttt aaagaaagtt attgcagact acagaataat caaagattat
      181 aggatggtca aaggaagtaa tttttatata tcattaaatg tttcatttaa agaaatagaa
      241 aatcctaagt ttttaagatc cttaatgaaa attgcaaaag attataagat tgatgattgt
      301 aatatttgtt tagagatagt agaaaaattt ggtatggagg atataggaag aatacagagt
      361 gcaataagaa taataaagga atatggattt ttaatagcta tagatgattt tggtgtggag
      421 tattctaatt tagatttatt aaataaaatt gattctgata tagtaaagct agacaagtac
      481 tttgctgata atttagataa gtctattata aataaaaaaa cagtggaatt tatatcagaa
      541 atatgtaata tatcaaatag aaccatagta tttgaaggag tagagggaaa atatcaggtt
      601 aatattatta aggcatttcc atatgaaaaa atatatattc agggatattt ctattcaaag
      661 ccagtggata ttgagacttt aaaggatttt gaagttaagg atagttaatc ttcaaaggat
      721 agaaatagta agaattaagc agaagaattt atagtataat aaagtggtta taattagg
//
LOCUS       DAEVGI010000350          777 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEVGI010000350 DAEVGI010000000
VERSION     DAEVGI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.352"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..746)
                     /locus_tag="K8M77_002642"
     CDS             complement(<1..746)
                     /locus_tag="K8M77_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087060.1"
                     /translation="MGNFFLESDFRAFDIDKSRSRDKVILERRRVAAEKLRELNDLGL
                     FEFMNSKGLSSHWEKQYITSITWPLKRVNDEYVDRIHLSYGKNRADVRKLIKVLNLFH
                     NIEESRIEDDVVFNYIPRIQLGLDSNSWNTALYLDKYGSIEQRNLVNKIRENYKLKER
                     FKNILMALEEKGYRLYLIKDNNPRSYEDETDYVEDIIKYTDSGEKYGLTIRKDYDKNH
                     VSNSRNEILDFVKNEFDNLMDVYYFISWDS"
BASE COUNT          254 a          143 c           57 g          323 t
ORIGIN      
        1 tctgaatccc aagaaataaa ataataaaca tccattaaat tatcaaattc atttttaaca
       61 aaatctaata tttcatttct tgaattacta acatgatttt tatcataatc ttttcttatg
      121 gttaatccat atttttctcc actgtctgta tattttatta tatcctcaac ataatctgtt
      181 tcatcttcat aacttctagg attattgtcc ttaattaaat ataatctata tcccttttct
      241 tctaaagcca taagtatgtt tttaaatctt tcttttagct tatagttttc tcttatttta
      301 ttaacaagat ttctttgttc tatagatcca tacttatcta aatatagagc tgtattccaa
      361 gaattagagt ctaatcctaa ttgaatgcga ggaatataat taaataccac gtcatcttct
      421 attctacttt cctcaatatt atggaataaa tttaaaacct ttataagctt tctgacatca
      481 gctctatttt ttccatatga taaatgtatt ctatcaacat attcatcatt aactcttttt
      541 agtggccaag ttatactggt tatatattgc ttttcccaat gagagcttaa ccccttagaa
      601 ttcataaact caaaaagtcc taaatcattt aattctctta atttctcagc agcaactctt
      661 cttctttcta atattacctt atctctactt cttgatttgt ctatatcaaa agctctaaaa
      721 tcactttcta aaaaaaagtt tcccatattt ataccctcct taataaaaca tattaaa
//
LOCUS       DAEVGI010000351          772 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEVGI010000351 DAEVGI010000000
VERSION     DAEVGI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.353"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            53..>772
                     /locus_tag="K8M77_002643"
     CDS             53..>772
                     /locus_tag="K8M77_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBI7087061.1"
                     /translation="MKKRVSWVSIVFLIIIIGLAITIKFDCKKVNCNRKTIKVGFYEY
                     YPYYYLNKNSMPDGYYNEILELICNKINLNYEYVDCNITNALEKLKSGEVDLVFGISK
                     TPDREKDYEFTDHYINNDNFAIYTNKNIKNGDLKALNGLKMGFLKGEENNEWILRFLK
                     DKGINVKLIDVYNYPEDEEYLHDNKVDFIIENKRSNIDYENKNIKKIFEFSSGPVYIV
                     SRKGNKKLIERINSALGEIEDD"
BASE COUNT          337 a           45 c          129 g          261 t
ORIGIN      
        1 gtaaaatata ttaaagataa ggttatatga gatattaggg aagtgttact ttatgaaaaa
       61 aagggtgtct tgggtaagta tagtattttt aataattata attggtttag caattacaat
      121 taaatttgat tgtaagaaag ttaattgtaa cagaaaaaca attaaagtag gattttatga
      181 atattatcct tattattatc ttaataaaaa ttctatgcca gatggctatt ataatgaaat
      241 attagaatta atatgtaata agataaattt aaattatgag tatgtagatt gtaatataac
      301 aaatgcttta gaaaagctta aatctggaga agtagattta gtttttggaa taagtaagac
      361 ccctgataga gaaaaggatt atgaatttac tgaccattac ataaataatg ataactttgc
      421 catatatact aataaaaata taaaaaatgg tgatttaaaa gctttaaatg gattaaaaat
      481 ggggttttta aaaggagaag aaaataatga gtggatttta aggtttttaa aagataaagg
      541 cataaatgtg aagcttatag atgtatataa ttatcctgaa gatgaagaat atttacatga
      601 taacaaagtt gattttataa tagaaaacaa aagaagtaat atagattatg aaaataaaaa
      661 tattaaaaaa atttttgagt tttcttctgg accagtttat atagttagta gaaaaggaaa
      721 taaaaaatta attgaaagaa taaattccgc tcttggagag attgaagatg at
//
LOCUS       DAEVGI010000352          763 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEVGI010000352 DAEVGI010000000
VERSION     DAEVGI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.354"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..695)
                     /locus_tag="K8M77_002644"
     CDS             complement(<1..695)
                     /locus_tag="K8M77_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBI7087062.1"
                     /translation="MGLTLSLKDIIIRLLLATIVGGVIGYEREYHNRAAGFRTQILVC
                     VGAAIVSLIEIRLGQDVFEFVKDNYYIRSVIKIDYARLGSQFISGIGFLGAGTILQNK
                     GSVKGLTTAASLWVVACLGLAIGMGYYNLGILGFLFIFLTLVSLKKFQSKFITKAGEK
                     CFEIIYEKDSQVLEFIDKIFTQKGVKIKSIEFFGRNNNEAVQYKNAIYRVELPNYIEV
                     CEIINKLSINDGI"
BASE COUNT          267 a          146 c           66 g          284 t
ORIGIN      
        1 ataattccgt catttatact taatttattt attatttcac aaacttctat atagttaggt
       61 aattcaactc tatatatagc atttttatat tgcacagctt cattattatt tcttccaaaa
      121 aactctatgc tttttatctt tacacctttt tgagtgaaaa tcttatctat aaactctaaa
      181 acttgtgaat ctttctcata tattatttca aaacattttt ctccagcctt ggttataaat
      241 ttagattgaa acttctttaa gcttactaag gttaagaata tgaataaaaa tcctaaaatg
      301 cctaagttat aataccccat accgatagct aaaccaagac aagccacaac ccatagtgat
      361 gcagcagttg ttaacccttt tactgatcct ttattttgta atattgttcc tgcgcccaaa
      421 aagccaattc ctgaaatgaa ctgagatcct aatctagcat aatcaatttt tattacactt
      481 cttatataat aattatcttt tacaaattca aagacatcct gacctaatct tatttctatt
      541 aaagaaacta tagctgcgcc tacacaaact aatatttgtg ttctaaatcc agcagctcta
      601 ttatgatact ccctttcata acctataacc cctccaacta ttgttgctaa taacaacctt
      661 ataattatat ctttaagact taaagttaat cccataataa actcctcaaa tactttaaat
      721 gaacttatta taagatatgc taaataaatg ttaaaatatt tag
//
LOCUS       DAEVGI010000353          759 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEVGI010000353 DAEVGI010000000
VERSION     DAEVGI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.355"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            72..>759
                     /locus_tag="K8M77_002645"
     CDS             72..>759
                     /locus_tag="K8M77_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBI7087063.1"
                     /translation="MLNKNGENLDMLIGLFNNLGIKDKTLKRIAIVTVILLSLFFIKG
                     LNIIYALNRDLFKDVSVVYAAEEIIVMTQIILCIMILSICFIYYRGLKRKEFFGISLV
                     YVSIITEMIFIVLTGKIVENQVYDLNLFSLLFRGILLLLAILCLENFDAFIRKYKKLT
                     LVLVILVTIILQILNVNYNIGIYTYNFIYNLTLVLIVLTYISCIIFCLVRIFQFREIT
                     YLVIMISSSLM"
BASE COUNT          287 a           53 c          100 g          319 t
ORIGIN      
        1 taataaattt ttgaaaaaaa gagttatttt tacttatttt gaatatttgt ttaatatata
       61 atagaagtac tatgctaaat aagaatgggg aaaatttaga tatgttaatt ggacttttta
      121 acaatttagg tattaaagat aaaacattaa aaagaatagc aatagtcaca gtaatattac
      181 ttagtttatt ttttataaaa gggttaaata ttatttatgc cttaaataga gatttgttta
      241 aggatgtaag tgtagtttat gctgctgaag aaattatagt aatgactcaa ataattcttt
      301 gcattatgat attatctata tgctttatat attatagagg acttaaacga aaagagttct
      361 ttggtatatc ccttgtttat gtaagtatta ttactgaaat gattttcata gtcttaacag
      421 gaaaaatagt tgaaaatcag gtttatgact tgaatttatt tagtttatta tttagaggga
      481 tattactact tttagctatt ttatgccttg aaaattttga tgcttttata aggaaatata
      541 aaaaattaac attagtactt gtaatattgg taactattat attacaaata ttaaatgtca
      601 attataacat aggaatatat acatataatt ttatatataa tttaacttta gttttaatag
      661 ttttaacata tatatcttgt ataatatttt gtttagttag aatttttcaa tttagagaaa
      721 taacttattt agtaataatg ataagttctt cacttatgt
//
LOCUS       DAEVGI010000354          753 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEVGI010000354 DAEVGI010000000
VERSION     DAEVGI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.356"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            246..749
                     /locus_tag="K8M77_002646"
     CDS             246..749
                     /locus_tag="K8M77_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBI7087064.1"
                     /translation="MMDRNIKRIVISALIAALYATLTIVLAPISYLGVQFRISEIMVL
                     LAFYKKDYIVGLTLGCFIANILGPNGTVDVILGTFATFISVWAIYLTGKYIKGKKAIW
                     ISSVWPTIFNGIIIGWMLNYVYGFPLIISIGQVALGEFVVITLIGVPVFNFISKKYFN
                     KLNIIIK"
BASE COUNT          274 a           79 c          139 g          261 t
ORIGIN      
        1 tctgatgaag aataagcatt tatctaaagc tattgcaagt cagaagttct ttaaatttaa
       61 aactaagtta acagttaaat gtaaagaaaa tcatatagaa cttatatatg tacggagggc
      121 tagcttcgga atttaagcct ttggagagcc atacaaatcg tagtagctta ggcaaggcga
      181 ggctttatga agaaggaata atctccatat ggatatgttt gaccatattt tgagtagcag
      241 ggaatatgat ggatagaaat ataaaaagaa tagttatatc agcacttatt gcagcattat
      301 atgcaacact aacaatagtt ttagctccta taagttattt aggagtacaa tttaggatat
      361 cagaaattat ggtgctttta gccttttaca agaaagatta tatagttgga ttgacattag
      421 gatgtttcat agcaaatata ttaggaccaa atggtactgt tgatgttatt ttaggaactt
      481 ttgcaacatt tataagtgtt tgggcaatat atttaactgg taagtatata aaaggaaaga
      541 aagctatatg gatttcttca gtttggccaa caatatttaa tggtattata ataggatgga
      601 tgcttaatta tgtttatgga ttccccctaa ttatatcaat agggcaagtt gctttaggtg
      661 aatttgttgt aataacatta ataggagtac ctgtgtttaa ttttataagc aaaaaatatt
      721 ttaataagtt aaatattata ataaaataat ttt
//
LOCUS       DAEVGI010000355          752 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEVGI010000355 DAEVGI010000000
VERSION     DAEVGI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.357"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            55..672
                     /locus_tag="K8M77_002647"
     CDS             55..672
                     /locus_tag="K8M77_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HBI7087065.1"
                     /translation="MSKMKKCKVCSKEIASSAKSCPGCGAKNKKAIYKRPWFIILAII
                     IILGAVGGSSHDKSKVANNETISEVDQNQDKEISNNQGGKKEVLVKENEEKLEKKEVK
                     VEKVPKEYKAALRKAKVYSDTMSMSKAGLYDQLTSEYGEKFSAEAAQYAVDNLNANWK
                     ENALKKAKVYQETMAMSPSAIYDQLVSEYGEKFTSGEAQYAVDNL"
BASE COUNT          332 a           69 c          140 g          211 t
ORIGIN      
        1 atatttttat aaaatcttaa aatataaaca tctaatttaa aggggaagtt tgaaatgtca
       61 aagatgaaaa aatgtaaagt ttgttctaaa gaaatagcat ctagtgctaa atcttgtcct
      121 ggatgtgggg caaaaaataa aaaagcaata tataaaagac catggtttat aatattagca
      181 ataattataa tacttggtgc tgtaggagga tcaagtcatg ataaatcaaa agtggctaac
      241 aatgaaacta taagtgaagt agatcaaaat caagataaag aaattagtaa taatcaaggc
      301 ggaaaaaaag aggttttggt taaagagaat gaagaaaaat tagagaaaaa agaagtgaag
      361 gtagaaaaag ttccaaaaga atacaaggct gcattaagaa aagcaaaggt atatagtgat
      421 actatgagca tgtctaaagc aggattatat gatcaattaa catcagagta tggagagaag
      481 ttttctgctg aagcagctca atatgctgta gataatttaa atgcaaattg gaaagaaaat
      541 gcattaaaaa aagcaaaagt atatcaagag actatggcaa tgtctccaag tgcaatatat
      601 gatcaattag tttcagaata cggagaaaaa tttacatcag gagaagctca atatgctgta
      661 gataatttat agtattatat ttaaaagtta tgacttagtt ttaaataaag atttttggat
      721 ttaatacaag agatatagaa attttctata tc
//
LOCUS       DAEVGI010000356          737 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEVGI010000356 DAEVGI010000000
VERSION     DAEVGI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.358"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            12..632
                     /locus_tag="K8M77_002648"
     CDS             12..632
                     /locus_tag="K8M77_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBI7087066.1"
                     /translation="MKVLLVNGSPNREGCTYTALKEVSKTLEENGIDTEIFQVGNKPI
                     GGCIGCGSCKKTGECFMKDCVNEFVEKAKGADGFVFGSPVYYAAASGSITSFLDRAFY
                     SGGKHMAFKPGAVICSARRAGTTSTLDQLSKYLTICNMPVVSSQYWNMVHGNTPEEVK
                     KDLEGMQTMRVLGRNMAWLIKCIDLGKKNNILKPELEERERTNFIR"
BASE COUNT          278 a           81 c          162 g          216 t
ORIGIN      
        1 ggtacaaaaa aatgaaagta ttattagtta atggaagtcc aaatagagag ggatgtactt
       61 atacagcatt aaaggaagta agtaaaactc tagaggaaaa tggaatagat actgaaatat
      121 ttcaagtggg aaataagcct ataggtggat gcataggatg tggctcttgc aaaaaaacag
      181 gggaatgttt tatgaaggat tgtgtaaatg aatttgtaga aaaagctaag ggtgcagatg
      241 gatttgtatt tggttcacca gtttattatg ccgcagcttc aggatcaata acttcatttt
      301 tagatagagc attttattct gggggtaaac atatggcctt taagccaggg gcagttatat
      361 gtagcgcaag acgtgctggg acaacctcta ctttagatca attgagtaag tatcttacaa
      421 tatgcaatat gccagttgta agttcacaat actggaatat ggttcatgga aatactccag
      481 aggaggttaa aaaggaccta gaaggtatgc aaacaatgag agttctagga agaaatatgg
      541 catggcttat taaatgtata gacttaggta agaagaacaa cattcttaaa ccagaattag
      601 aagaaagaga aagaactaat ttcatcagat aatatgaaga tttaaaaata gtttttataa
      661 atagactctg ttagttaaga gagtaaataa gtggaatgta tcaaaagtaa ttttaatgtt
      721 tgatatattt cactttt
//
LOCUS       DAEVGI010000357          737 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEVGI010000357 DAEVGI010000000
VERSION     DAEVGI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.359"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(378..>737)
                     /locus_tag="K8M77_002649"
     CDS             complement(378..>737)
                     /locus_tag="K8M77_002649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087067.1"
                     /translation="CFLYLKEKNLFKVFKSNNIFEDLVIIGEVLWCEQYDFKKLNELK
                     YNDFDRFYYLDNKEIEFIKSFYEFITMYLSDPHMTYSQKEEFLKKFGIVDYYEAAREI
                     ENEIFEEFIKQQEELAK"
BASE COUNT          284 a          104 c           45 g          304 t
ORIGIN      
        1 ttataagccg cccctatagt tttaaaaaaa aatcattatc aaaatctaca atacaggacc
       61 tagggtagtt atttcaacgg taccaaatga ttttttttgt taaaaatgca caaaaataga
      121 cctcacaaaa atttgtgaag tctaaatttt ttaatttgtt actatattta tattatatac
      181 aataccccct catttgtaca ctattttttt aaattcacct taatatttta tattataact
      241 acagtcttat gtttatttat aaacttataa taatactttt aacattaaat atttttacta
      301 atagattatt aaaaattaat attacatatt taaataaagt atcaaaaaat acaaattaat
      361 ctactgttta aatttaatta cttagctaac tcttcttgtt gttttataaa ttcttcaaaa
      421 atttcatttt caatctccct ggccgcttca taataatcaa caattccaaa tttttttaaa
      481 aattcttctt tttgcgaata agtcatatgt ggatcactta aatacattgt aatgaattca
      541 tagaaacttt ttataaattc aatctcttta ttatctaaat aataaaatct atcaaaatca
      601 ttatacttaa gttcatttaa tttcttaaag tcgtattgtt cacaccataa tacttctcca
      661 ataattacta aatcttcaaa aatattattt gatttaaata ctttaaataa atttttttct
      721 tttaaatata aaaaaca
//
LOCUS       DAEVGI010000358          733 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEVGI010000358 DAEVGI010000000
VERSION     DAEVGI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.360"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            20..>733
                     /locus_tag="K8M77_002650"
     CDS             20..>733
                     /locus_tag="K8M77_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBI7087068.1"
                     /translation="MKKNKRFIILILFFLIFIILFLLHGVIINKEKREQVVKIGFYDD
                     YPHFYIDHRGNVCGYYKDITEKLAEKLNFKIEYVNGKVPELLKELKNGEIDLVFGINK
                     LPEREELFDFTNNPINNEMKLIYTNNDIKYGDLEGLNGMKMGYIEDELDNEWILDYLS
                     KRNINVELVNGSSYKAIKTLLVENKVDFIVDNPDSDIKNKGKNIKEIFQFSSGPKYIV
                     ANKNNNELIKKIDKSLTTIN"
BASE COUNT          320 a           57 c          115 g          241 t
ORIGIN      
        1 atatagttgg agttaattta tgaaaaaaaa taaaagattt ataattctta tattattttt
       61 tttaatattc ataatactat ttttgcttca tggagtcatc ataaataaag aaaagagaga
      121 gcaagtagtt aaaattggat tttacgacga ttatcctcat ttttatatag atcatagagg
      181 aaatgtttgt ggatattata aagacataac tgaaaaatta gctgaaaaac ttaattttaa
      241 aattgaatat gtaaacggaa aagttccaga acttttaaaa gagcttaaaa atggagaaat
      301 agatttggtt tttggaataa ataaattgcc agagagggag gaactctttg acttcacaaa
      361 taatcctata aataatgaga tgaagcttat atatactaat aatgacataa aatatggtga
      421 tttagaaggc ttaaatggta tgaaaatggg gtatatagaa gatgagttag ataatgaatg
      481 gatattagat tatttaagca aaagaaatat aaatgttgaa ttagttaatg gatcttctta
      541 taaagcaata aaaaccttgt tagttgaaaa taaagtagac tttattgtag ataatccaga
      601 tagtgatata aaaaataagg gtaaaaatat taaagagatc tttcaatttt catctggacc
      661 aaaatacatt gtagcaaata aaaataataa tgagttaata aaaaaaattg ataaatcact
      721 tactacaatt aat
//
LOCUS       DAEVGI010000359          732 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEVGI010000359 DAEVGI010000000
VERSION     DAEVGI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.361"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>732
                     /locus_tag="K8M77_002651"
     CDS             <1..>732
                     /locus_tag="K8M77_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI7087069.1"
                     /translation="ILLEKKWIDFRNDSIESFNFPFKSYRTGQRELAIRVYKSITEGK
                     KCFAEAPTGTGKTMSTLFPAVKALGAKKTQKIFYITAKTTTRKIANNNLRIMREKGLN
                     LRSVNITAKEKCCKMEEKKCIPEYCPYANGYFDRVNIALKEALRHKEEYDLDYINKLS
                     EEYNICPFEFSLELSNFCDVVICDYNYIFDPQASLKGILEGKAKDYTILIDEAHNLLD
                     RGRSMYSSKIFKDDFLKLKREFKSK"
BASE COUNT          291 a           75 c          135 g          231 t
ORIGIN      
        1 gatactttta gagaaaaaat ggatagactt tagaaatgat tctatagaaa gttttaattt
       61 cccttttaag tcttatagga cggggcagag agaattagct attagagttt ataagtctat
      121 tacagagggg aaaaaatgtt ttgcagaagc acctactggc actgggaaaa caatgtctac
      181 attatttcct gcagtaaaag ctttaggagc taagaaaact caaaaaattt tttatataac
      241 agctaaaact accacaagaa aaattgcaaa taataatttg cgtattatga gagaaaaagg
      301 attaaatcta agaagtgtta atataactgc aaaagaaaaa tgttgcaaga tggaagaaaa
      361 aaagtgtatt ccagaatatt gtccttatgc taatggatac tttgataggg ttaatattgc
      421 tttaaaggaa gcactaaggc ataaggaaga atatgattta gactatataa acaaattgtc
      481 tgaggaatat aatatttgtc cctttgaatt ttctcttgag ttaagtaatt tttgtgatgt
      541 agtaatatgt gattataatt acatatttga tcctcaggct tctttaaagg gaatattaga
      601 agggaaagcc aaggattata caattttaat agatgaagct cataatcttt tagatagagg
      661 aagaagtatg tattcttcaa aaatatttaa agatgatttt ttaaagctaa aaagagaatt
      721 taaaagcaag ga
//
LOCUS       DAEVGI010000360          726 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEVGI010000360 DAEVGI010000000
VERSION     DAEVGI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.362"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(136..>726)
                     /locus_tag="K8M77_002652"
     CDS             complement(136..>726)
                     /locus_tag="K8M77_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBI7087070.1"
                     /translation="KPLDYPYDALEPSIDEETVKIHHDKHQQAYVDKLNKALEKHPEL
                     YGKSLYDILSNLDDMPEDIMADLVNQGGGVYNHEFYWSILGKGCNRPVAEIADAIDRD
                     FGSFEEFKEKFKQCGISTFGSGWAWLVSDKDGKLEIMSTKDQSSPISLGLIPILTMDV
                     WEHAYYLKYQNKRPEYIDYFFDIINWKKCEEYYNNR"
BASE COUNT          230 a          132 c           87 g          277 t
ORIGIN      
        1 atgtatacta caataatttt acaaatatta ccatatgata tttaaaaaag cgtgtattaa
       61 agtaaatttt atttaaaaac cactttaata cacgctcttt ctatgattta ttacatatta
      121 tatgatattt ttctattatc tattattata atattcttca cactttttcc agtttattat
      181 atcgaagaaa tagtctatat actctggacg tttattttga tattttaagt aatacgcatg
      241 ttcccaaaca tccattgtta gtataggaat taaccctaaa gaaataggag atgattgatc
      301 tttagttgac atgatttcta actttccatc tttatcacta actaaccatg cccaacctga
      361 accaaaggta gagattccac attgcttaaa cttttcttta aattcttcaa aagaaccaaa
      421 atctctgtct atagcatctg ctatttcagc aactggtcta ttgcatcctt ttcccaaaat
      481 actccagtag aattcatggt tgtaaactcc accaccttga tttactaagt cagccataat
      541 atcctctggc atatcatcta aattgcttaa gatatcatat aaactttttc cataaagctc
      601 cggatgtttt tctaaagctt tatttaactt atctacgtaa gcttgttggt gcttgtcatg
      661 gtgaattttt actgtttcct catctattga tggctctagg gcatcataag gatagtctaa
      721 aggctt
//
LOCUS       DAEVGI010000361          725 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEVGI010000361 DAEVGI010000000
VERSION     DAEVGI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.363"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>725
                     /locus_tag="K8M77_002653"
     CDS             <1..>725
                     /locus_tag="K8M77_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI7087071.1"
                     /translation="LYMKKRDFKNCENKLYSIVNNKDMFYSLPDNLKFEIYNYLAIIN
                     LQQEKFLNALSYYEEAFKYADKESKIIIKLNMTSAYRYMGAYVTATNILNKMIESSLT
                     FKNENSYLKEYTLLNLAETYFAVNDITDFNSTIETISKYYCGPENELADLKILLDSYL
                     IIKAISENNLDLVPKYISEIDELESKNKDLIYSELEMIKLRSYGMYYESIGNFNLALD
                     YFSKLEKSADNEGASYVSLFSIS"
BASE COUNT          295 a           76 c           92 g          262 t
ORIGIN      
        1 attatatatg aaaaaaagag attttaaaaa ttgtgaaaat aaactttact caattgtaaa
       61 taataaagat atgttttata gtttacctga taatttaaag ttcgagattt ataattattt
      121 agctataata aatcttcaac aagaaaaatt tttaaatgct ttatcttatt atgaagaagc
      181 ctttaaatat gctgataaag agtcaaagat aattattaag ctaaacatga catctgctta
      241 tagatatatg ggggcctatg ttactgctac taatatttta aataaaatga tagagtcatc
      301 attaactttt aaaaatgaaa attcttatct taaagaatat actctattaa atttagctga
      361 aacttatttt gctgtaaatg atataactga ctttaattct acaatagaaa ctatatcgaa
      421 atattattgt gggcctgaaa atgagttagc agatctaaaa atacttttag attcctattt
      481 aataataaaa gcaatatcag agaataacct tgatttagta ccaaaataca tttctgaaat
      541 agatgaatta gaaagtaaaa ataaagatct catatattca gaattagaaa tgattaaact
      601 tcgttcatat ggtatgtatt atgaaagcat aggaaatttc aatttagcat tagattattt
      661 ttccaaatta gaaaaatcag ctgataatga aggtgcttct tatgtttcgc ttttttctat
      721 aagca
//
LOCUS       DAEVGI010000362          724 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEVGI010000362 DAEVGI010000000
VERSION     DAEVGI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.364"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..558
                     /locus_tag="K8M77_002654"
     CDS             <1..558
                     /locus_tag="K8M77_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087072.1"
                     /translation="SLVYENGEFTVYINDEVVSVNKYMDNAIEKFTQTVHNNATPKSI
                     KWESIEEDLKGIDLKDLEINSEFKTLTYKDMKYFYSTDKIFNMHGGRMQQLLGGYQLF
                     SFIVKMISEKHLEDYLEVLNFCEDILRCKVTYRTQGSNFIVGSPAFNYGSASYDFATG
                     KVNKGASIEKMSFKDFKKYIFDIIK"
BASE COUNT          293 a           63 c          119 g          249 t
ORIGIN      
        1 agcttagtat atgaaaatgg agagtttact gtctacataa atgatgaagt agtttcagtt
       61 aataaatata tggataatgc aatagagaaa tttactcaaa cagtgcataa taacgctaca
      121 ccaaaatcaa ttaagtggga aagtattgaa gaggatttaa aaggaattga tttaaaagat
      181 ttagagataa atagtgaatt taaaacatta acttataagg atatgaaata tttctacagt
      241 acagataaaa tctttaatat gcatggtggg agaatgcaac aattattagg aggatatcaa
      301 ttattctcat ttatagttaa gatgatttct gaaaaacatt tagaagatta tttggaagtt
      361 ttaaactttt gtgaagatat cttaagatgt aaggttacat atagaactca aggaagtaac
      421 tttatagttg gtagcccagc ttttaactat ggatcagcat catatgattt tgctactgga
      481 aaagttaata agggagcttc aatagaaaag atgtctttta aagatttcaa aaaatatata
      541 tttgatataa taaaataacc tttggtattc caaaggttat ttttgatggt tttcttattt
      601 taagagttta aaagtataat taatataaat aaaattttta atttaaatgc ttaattgtaa
      661 aatgaaattg aactaaataa aaaatccatg gtggattaac ctgtgttatg atttaatcgc
      721 aaaa
//
LOCUS       DAEVGI010000363          723 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEVGI010000363 DAEVGI010000000
VERSION     DAEVGI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.365"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>723
                     /locus_tag="K8M77_002655"
     CDS             <1..>723
                     /locus_tag="K8M77_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7087073.1"
                     /translation="FYIAHNKEVYEKEGKFVSGMDFSKWLNNEYIPNNQDMKWIKEVS
                     SKATKQAIMNGDKAFRDFFKKSKGFPKFKKKKNQDVKAYFPKNNKTDWTLERHRVKIP
                     TLGWVRLKEFGYIPVNSIVKSGTVSQKADRYYVSILVEERDIKISNSNIGIKISNTNN
                     EGLGIDLGIKEFAICSNGNKFKNINKNSTVKKIEKKLKREQRKLSRKYESLKIRNKNL
                     KEGRATRQNIQKQVVKVQRLHQ"
BASE COUNT          335 a           64 c          121 g          203 t
ORIGIN      
        1 cttttatata gctcataata aagaagttta tgagaaagaa ggaaagtttg taagtggtat
       61 ggatttttca aaatggttaa ataatgaata tatcccaaat aatcaagata tgaaatggat
      121 taaagaagta tcttcaaaag ctactaaaca agctattatg aatggagata aagcttttag
      181 agatttcttt aaaaaatcta aaggctttcc taagttcaag aaaaagaaaa atcaagatgt
      241 aaaagcttat tttcctaaga ataataagac tgattggact ttagaaagac atagagtcaa
      301 aataccaact ttaggatggg taagattaaa agaatttggt tatattcctg taaattcaat
      361 agtcaaaagt ggtacagtaa gtcaaaaagc tgatagatat tatgtatcta tattggttga
      421 agaaagagat ataaaaatat ctaatagtaa tataggtata aaaatatcta atacaaataa
      481 tgaaggctta ggaattgacc taggtattaa ggaatttgca atatgttcaa atggtaataa
      541 atttaaaaat ataaataaaa attcaacggt taagaaaatc gaaaagaagt taaaaagaga
      601 gcaaagaaaa ctttcaagga aatatgaaag tttaaaaata agaaataaaa atttaaaaga
      661 agggagagct actcgtcaaa atattcaaaa acaagtagta aaagtacaaa gacttcatca
      721 gag
//
LOCUS       DAEVGI010000364          694 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEVGI010000364 DAEVGI010000000
VERSION     DAEVGI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.366"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            262..>694
                     /locus_tag="K8M77_002656"
     CDS             262..>694
                     /locus_tag="K8M77_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI7087074.1"
                     /translation="MDLKALNPNGNAFLVSFTKAKKTYKKFISPTLNDLGLTHNEFEI
                     LVFLQENLEYNTAKDIVEFSGLSKGLISRSIESLLKKEMLSIKKDDKDKRIFRLYISP
                     SANETLNTLNKLSNKFFKLLLEDLKYEELVLFDNILNKMIRN"
BASE COUNT          288 a           66 c           70 g          270 t
ORIGIN      
        1 aaatcatatt tttatctttt atatttttcc ttttaataaa caactttata gaatttttag
       61 catttttcaa catttaatca ttatattatt aaataaataa aacattattt tttctcatta
      121 agcaaaaaat attattaaaa agtatatttt catatgtata atgtttatat gctttaaata
      181 ataatatata gtttacttat aaactaattt aaggtataat ttatataaag tttacaattg
      241 aactatcaag gaggaaattt aatggattta aaagctctta atcctaatgg aaatgctttt
      301 ttagtttctt ttactaaagc aaaaaaaaca tataaaaaat ttatttcacc aacattaaat
      361 gatttgggat taacgcataa tgagtttgaa atattagttt tcttacagga aaatttagaa
      421 tataatactg caaaggatat agttgaattt tctggccttt ctaagggatt aatttcaaga
      481 tctatagaaa gcttattaaa aaaggaaatg ctatcaatca aaaaagatga caaagacaaa
      541 agaatattta gattgtatat atctccatct gctaacgaaa cacttaatac tttaaataaa
      601 ttaagtaata aattttttaa gctactctta gaagatttaa aatatgaaga acttgttctt
      661 tttgataata ttttaaataa aatgattaga aatt
//
LOCUS       DAEVGI010000365          681 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.367, whole genome shotgun sequence.
ACCESSION   DAEVGI010000365 DAEVGI010000000
VERSION     DAEVGI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.367"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(72..662)
                     /locus_tag="K8M77_002657"
     CDS             complement(72..662)
                     /locus_tag="K8M77_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI7087075.1"
                     /translation="MTKETHSTGGVCISLIMLNTILSLFLFPYDLAYKILLIALFFHF
                     SYIGSLFPDIDQRKSSISQMYPFLSKHIGSKCRHRGFTHSLLCLSLTILTLYIVLNIS
                     NFNIILLIISIGFIAGYISHLILDFLTSEGIELFYPWKTNFKIAKIKTGSKTEKIINK
                     VLKIFNFLLIIYNIALILSDILGINILSFISKSTLF"
BASE COUNT          273 a           67 c           83 g          258 t
ORIGIN      
        1 aacttataat attagtttat gaaattttta ttatgttgtt ccaaaatgtt ttatataaaa
       61 agtaaaagca ctcaaaatag agtgctttta cttataaaac ttaatatatt tattcctaaa
      121 atatcagata aaattaaggc tatgttatat ataattagaa ggaaattaaa tatttttagt
      181 actttattta ttatcttttc agtttttgat cctgttttaa ttttagctat tttaaagtta
      241 gttttccaag gatagaaaag ttctattcct tctgaagtta aaaaatctaa aattaagtgg
      301 gatatatatc cagctataaa tcctattgaa attattaata atattatgtt gaagttacta
      361 atatttaaaa caatatataa ggttaatata gttaaagata gacatagtaa actatgagta
      421 aagccacgat gtctacattt actacctata tgctttgaaa gaaaaggata catttgacta
      481 attgaagatt ttctttgatc tatatcagga aatagagatc ctatataaga aaaatgaaaa
      541 aataatgcta ttaataaaat tttataagct agatcatatg gaaataagaa taggcttaaa
      601 attgtattta gcattattaa agaaatgcat acgcctccag ttgaatgagt ttcttttgtc
      661 ataaaatctc ctttaaaaat a
//
LOCUS       DAEVGI010000366          680 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEVGI010000366 DAEVGI010000000
VERSION     DAEVGI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.368"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            114..581
                     /locus_tag="K8M77_002658"
     CDS             114..581
                     /locus_tag="K8M77_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBI7087076.1"
                     /translation="MSIEINKLAPDFKLMGSDGKEHSLSDYKGKNIVLYFYPKDNTAG
                     CTTEACDFRDNIQNFHDLNAIILGVSRDSLASHDKFITKLSLPFVLLSDPDEIVCKLY
                     DVIKEKNMYGKKYMGVERSTFLINKEGILIEEFRKVRVKGHIEKVLDKLKEIN"
BASE COUNT          291 a           59 c           89 g          241 t
ORIGIN      
        1 taaaatttta atttttatat ttaaatttta tacatatgaa tatctctctt ctactttgtt
       61 gataataaat agtaattatt atataatata tataatttaa aggagggatt cttatgtcaa
      121 ttgaaataaa taaattagct ccagatttta aacttatggg ttctgatgga aaagaacatt
      181 cactaagtga ttacaaagga aaaaatatag ttttatattt ttatcccaag gataacactg
      241 ctggttgtac tacagaagct tgtgatttta gagataatat acaaaatttt catgatttaa
      301 atgcaattat tttaggtgtt tctagagatt ccttggcatc tcacgataaa tttattacta
      361 aattatcact tccatttgta ttgcttagtg atccagatga aatagtttgt aaattatacg
      421 atgtaataaa agaaaaaaat atgtacggca aaaaatatat gggtgtagaa agaagcactt
      481 tcttaataaa taaagaagga atattaatag aagagtttag aaaagtaaga gttaaaggac
      541 acatagaaaa agttctagat aaattaaaag aaataaatta aaaaataaaa ttaaaatatg
      601 atcttatatc aagataatat tataacttct tattttaaat aaaaatttaa aatttatata
      661 caaaaaaagc ttagtgaata
//
LOCUS       DAEVGI010000367          667 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEVGI010000367 DAEVGI010000000
VERSION     DAEVGI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.369"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>667
                     /locus_tag="K8M77_002659"
     CDS             <1..>667
                     /locus_tag="K8M77_002659"
                     /inference="COORDINATES: protein motif:HMM:NF020452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HBI7087077.1"
                     /translation="CPKKYKEYISSDKSGSLSRSSRKAFRQYISVRRSINNGNSLPKT
                     TDGELGELMLYSFVESHLNAPKILTKMRFKTSNNDPVKRADGIHLLKLSDDYYELVYG
                     ESKLYENLSNGLTDAFSSINDFIKRDNNNINDEYTFLIDNIESEFSEEEYTIAKNILI
                     PNENPISIDNSFAIFVGFQINIPEDINKLKSINFQNKLHEHIKSEVTKRFDHIKNKID
                     EFNL"
BASE COUNT          278 a           82 c           87 g          220 t
ORIGIN      
        1 atgccctaag aaatataaag aatatatttc ttcagataaa tcaggctcat taagtagaag
       61 ttctagaaaa gcctttagac agtatattag tgtacgaaga agtataaaca atgggaattc
      121 acttccaaaa actacagatg gagaactagg tgaattaatg ttgtattcct ttgtcgaatc
      181 acatttaaat gcccctaaaa ttcttactaa aatgagattt aaaacttcta ataatgatcc
      241 tgttaaaaga gcagatggta ttcatctact taagttatct gatgattatt atgagcttgt
      301 atatggagaa tctaaacttt atgaaaattt atctaatgga ctaacagatg cattttcctc
      361 tataaatgat tttatcaaaa gagataataa taatataaat gatgaatata cttttttaat
      421 agataatata gaatctgaat ttagtgaaga agaatatact atagcaaaaa atatcctaat
      481 tcctaatgaa aacccaatca gcatcgataa ttcatttgct atatttgttg gatttcagat
      541 aaatatacct gaagatatta ataaattaaa atcaataaat tttcaaaata aattacacga
      601 acacatcaaa tctgaagtta ctaaaagatt tgatcatata aaaaataaaa ttgatgagtt
      661 taatcta
//
LOCUS       DAEVGI010000368          650 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEVGI010000368 DAEVGI010000000
VERSION     DAEVGI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.370"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(46..576)
                     /locus_tag="K8M77_002660"
     CDS             complement(46..576)
                     /locus_tag="K8M77_002660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087078.1"
                     /translation="MINLYLIDNEVCKGYLLAIINKKLNEKEEFNWNEFILYFLAVSK
                     GIIKPNNLLEIKFFKFIDNEIKEKQNNEKVGRHIFKIYNLDGLLSNALALLITERILN
                     KEYLKKYYKFNKCIEFLNENMPVNIFEVNWINRFSENINRELSKKEEFKNKVKEYLIE
                     NLDDTDTLKTYLKYYS"
BASE COUNT          238 a           90 c           35 g          287 t
ORIGIN      
        1 aaaaagctag aaagttaaaa actttctagc ttttaaaaat attctttatg aataatattt
       61 caaataagtt ttaagtgtat ctgtgtcatc taaattttcg attaaatatt cttttacttt
      121 atttttaaac tcttcttttt tagaaagctc tctatttata ttttctgaaa atctatttat
      181 ccaattaact tcaaatatat ttactggcat attttcattt aaaaattcta tacatttatt
      241 aaacttataa tatttcttta aatactcttt atttaaaatt ctttcagtaa tcaacaacgc
      301 taatgcatta gaaagtaaac catctaaatt ataaatttta aatatatgcc ttcctacttt
      361 ttcattattt tgtttctctt ttatttcatt atcaataaat ttaaaaaact ttatttccaa
      421 tagattattt ggcttaatta tacctttaga cacagctaaa aaatataata taaactcatt
      481 ccaattaaac tcttcttttt cattcaactt cttatttatt attgctaata agtaaccttt
      541 acaaacttca ttatcaatta gatataaatt tatcaatata ctataataga cataggtatc
      601 cttatttaaa tctatatatt cctttataat attgaaatct aaatcattta
//
LOCUS       DAEVGI010000369          648 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEVGI010000369 DAEVGI010000000
VERSION     DAEVGI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.371"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>648
                     /locus_tag="K8M77_002661"
     CDS             <1..>648
                     /locus_tag="K8M77_002661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087079.1"
                     /translation="ASEYDSYEVLKNNFIAKKKEYEQLSNEINLIDQNIRELEKGSKI
                     IEIFSLLVNNKDDIIDEYRNKGNINCPLCGSSEVFAKIKKDDIAKYSSEYLDKYDDIL
                     KSNFELKEKKKGALDGIWNSFNVFLDNFIKNEIKKLKENNSRIIGEWKRVKKFFELIN
                     KSSLTISKDIIKTIDNEIETKCNGILSKEDAIRIHEKVYEVLKYLQYAELDDIKM"
BASE COUNT          302 a           37 c          110 g          199 t
ORIGIN      
        1 atgcttcaga atatgattct tatgaagtat tgaaaaataa ttttattgca aagaagaaag
       61 aatatgagca attatcaaat gaaattaatt tgatagatca aaatataaga gaattagaaa
      121 aaggaagtaa aataattgaa atattttcat tgttagtaaa taataaagat gacattattg
      181 atgaatatag aaataagggc aatatcaatt gtccattatg tggatcttct gaagtatttg
      241 caaagataaa aaaagacgac atagcaaagt actcaagtga atatttagat aaatatgatg
      301 atattttaaa gagtaatttt gaattgaaag aaaagaaaaa aggagcttta gatggtattt
      361 ggaatagttt caatgtattt ttagataatt ttattaaaaa tgaaataaaa aaattaaaag
      421 agaataatag cagaataatt ggtgagtgga aaagggttaa aaagtttttt gagttaataa
      481 ataaaagtag tcttacaata agtaaagata taattaaaac aattgacaac gagattgaaa
      541 ccaaatgtaa tggaatatta tcaaaagaag atgctataag aatacatgaa aaagtctatg
      601 aagtgttaaa atatttacaa tatgctgagt tagatgatat taagatga
//
LOCUS       DAEVGI010000370          647 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEVGI010000370 DAEVGI010000000
VERSION     DAEVGI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.372"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..384)
                     /locus_tag="K8M77_002662"
                     /pseudo
     CDS             complement(1..384)
                     /locus_tag="K8M77_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          210 a          101 c           44 g          292 t
ORIGIN      
        1 gaaagttttc tttgttccct ttttaatttc ttttctactt ttttaacagt agaagtttta
       61 tttatatttt taaacttaat tccatcacta catacagcaa attccttaat acctagatca
      121 attcctaagc cttcattatt tgtgttagat attttcatac ctgtatttga attagatatt
      181 ttatacctat attactatta gatgttttta tatctctttc ttcaaccaat atagatacat
      241 aatatctatc agctttttga cttactgtac cacttttaac taatgaattt acaggaatat
      301 aaccaaactc ttttagtctt accatcctaa agttggtatt ttgactctat gtctttctaa
      361 agtccaatca gtcttattat tcttagaata actgatacat ttaataattc tgcaaattct
      421 ttaggcttat aatgtttact catattctta ataacccctt atataatcac atccttactt
      481 aataaaatta tttttaaact atattatcta ttttatcata ctaaaaacaa attatttctt
      541 aatttattct ttaaaatatc ttctttatta taaaaaatta aagctactaa ttttattact
      601 taaatcatat gtgtataaaa ttagtagctt tttattccta ttatttg
//
LOCUS       DAEVGI010000371          642 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEVGI010000371 DAEVGI010000000
VERSION     DAEVGI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.373"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>642
                     /locus_tag="K8M77_002663"
     CDS             <1..>642
                     /locus_tag="K8M77_002663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087080.1"
                     /translation="KKLVYLKYYISSKEIEDKSKELNIYEKKINELNNKTEELKDIKE
                     TLKKYTDDIGNRLKEIEQTEFEDVGPYLFRIFNKIIKHTNIDGFKFKRDSSRVQGESG
                     ISFLDLEENNIMNMFSEGQLGVFMISYFIGNILKRKNENLIESFFMDDITNCLDDINV
                     LSFIDIIKYLINDENNGMNQLFFATCNNNIEALFKNRMEGFNIPYKSIEFDSFG"
BASE COUNT          274 a           34 c          100 g          234 t
ORIGIN      
        1 taaaaaattg gtttatttaa aatattatat ttcatcaaaa gagattgaag ataaaagtaa
       61 ggaattaaat atatacgaaa aaaaaattaa tgaattaaat aataagactg aagaattaaa
      121 agatataaaa gaaacactta aaaaatacac tgatgatatt ggcaataggt taaaggaaat
      181 tgaacaaact gaatttgaag atgttggacc atatttattt agaatattta ataagattat
      241 taagcataca aatattgacg ggtttaaatt taaaagagat agttctagag ttcaaggtga
      301 atctggaata tcgtttttag atttagagga aaataatatt atgaatatgt ttagtgaagg
      361 gcaattaggt gtatttatga tttcttattt tataggtaat atattaaaaa gaaaaaatga
      421 aaatttaatt gaaagttttt ttatggatga tataactaat tgtttagatg acattaatgt
      481 tttgtctttt atagatatta ttaaatattt aattaatgat gaaaataatg gtatgaatca
      541 gttatttttt gctacttgta ataataatat agaagctctt tttaaaaata ggatggaagg
      601 atttaatatt ccatataagt caatagaatt tgattcattt gg
//
LOCUS       DAEVGI010000372          623 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEVGI010000372 DAEVGI010000000
VERSION     DAEVGI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.374"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          265 a           66 c           41 g          251 t
ORIGIN      
        1 agcgttgttt ataaaatttt taaaataagt ttcattatct tttaactttt ccttgaattc
       61 tttaaattca ttattactct cttccatttt tttatttaat attttaagtt ttttattttt
      121 atcaattata tcttgatgta aaattttata tggttttgta ataagcattt cagaaatcca
      181 aacgtatatt ataagatata ttaatatttt taatatatat attaacaata gcaaagcttc
      241 taaattaaaa atatatacta gagcatatat taatactaaa gatagattta ttataatgaa
      301 taactttaag taatttaata gattattctt aaaaatctta aaatctttta aaagatataa
      361 tataagcaaa agaagtactg tagatatcac cccaaacaca attacatcaa ataaacatat
      421 actaactaag gatattatag taaaaaaaac aactattaat ttcatattta aatttacata
      481 ttttacatat actattatca tgaagcaata aatactttct aaaacattac aaatagctaa
      541 tatttttaaa tttaaacact gaattatttc tttttcacca aagaaaaaca ttaaataaac
      601 aagtgatata aattttaata tag
//
LOCUS       DAEVGI010000373          602 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEVGI010000373 DAEVGI010000000
VERSION     DAEVGI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.375"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            278..520
                     /locus_tag="K8M77_002664"
                     /pseudo
     CDS             278..520
                     /locus_tag="K8M77_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
BASE COUNT          239 a           63 c          102 g          198 t
ORIGIN      
        1 gggagaaaaa agagctatta acgtaattaa aattgcactt gttaaaatct tttttgtatg
       61 tttcaaaaaa tcaccccttg cttaataaaa ataaatattt acttaagaaa tattacaagt
      121 gtattttaac aataatttaa atatgatgga atagtttgtg cgtaactggt caaaaaatag
      181 gtaaaagtgg gcaaagtatg caaaatgtaa tctttaatat ggaaaaattt attataatta
      241 acttgggaga attttaattt gaattggagg ttttattgtg aataaattat taaaatgggc
      301 tacagagata gattctattg ctcaagcagg attaacttac tctaaagatg tatatgatat
      361 agatagattt aatcaattaa agaatattgc agctgatatt attagtgaaa gcactaattt
      421 agagctacat aaagttaagg aagttttatt tgaagaaaga ggatatttaa cccctaaggt
      481 tgatgtaagg gctgctataa taaaagaaaa taaaatttta tgcctttggc aagaacaccc
      541 ttgatttcaa tcatgggatg aattgccacg acatatgcta aaagtataaa ccgtctaaca
      601 ct
//
LOCUS       DAEVGI010000374          599 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEVGI010000374 DAEVGI010000000
VERSION     DAEVGI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.376"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..311
                     /locus_tag="K8M77_002665"
     CDS             <1..311
                     /locus_tag="K8M77_002665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087081.1"
                     /translation="LRDIPEDRINFVKEILEHNYHEDRLFSIKGESINRYLNRIEDKL
                     GIERHSNYDIRRLIAQEKFDSYRKSGLSIKDVANKTSAWLSHGDNRNELLEKSYIKLK
                     "
BASE COUNT          247 a           48 c           89 g          215 t
ORIGIN      
        1 agctaagaga tattccagag gatagaataa attttgttaa agaaatatta gagcataact
       61 atcatgaaga tagattattt agtataaagg gagagtctat aaatagatat ttaaatagaa
      121 tagaggacaa gctaggaata gaacgacata gtaactacga tataagacgg ttaatagcac
      181 aagaaaaatt tgatagttat agaaaatctg ggttaagcat aaaagatgta gctaataaaa
      241 caagtgcttg gttaagtcat ggagataata gaaatgagct ccttgaaaag agctatataa
      301 aattaaaata aattcttctg atttttattg tttaaaatct tatcttaatt taaaatttat
      361 actaatactg tgattttatt tactggtaat tgaaaatcta gtatttactt tttattttta
      421 aattgcaaac atcaattacc aaaacactaa aaagaagata aattttatat ttatttctga
      481 agttccttat tttgagggca ttgaaaaaat atagggtata tcttcttttt atattaatta
      541 attttaagga gttaaatatt atgtttaatt tgtataatga atttttaaaa tatgaatat
//
LOCUS       DAEVGI010000375          598 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEVGI010000375 DAEVGI010000000
VERSION     DAEVGI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.377"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            132..443
                     /locus_tag="K8M77_002666"
                     /pseudo
     CDS             132..443
                     /locus_tag="K8M77_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479694.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          253 a           52 c           69 g          224 t
ORIGIN      
        1 gtataattat agtgtctttt acttatggac aaagcttatt tttttaattt tttaattcat
       61 agctaaaaga agtatcttaa aattttaaaa tacttctttt ttattttcac ttataaatga
      121 gatgatgatc tttgattaat ttatatagaa aaaagctatt aaactttctg caatgaaatg
      181 ataaaaatgc ttattatact gacgaaagat gtgacttgga agaagttcaa aaattatcat
      241 tagaagaaag tataaaatat ttttttggag tgataaattc agatttttat tattcaatag
      301 ctgataatat ctttgaatta agcttttatg aaacaataaa atatgctaaa gattataagt
      361 tttataatca aacgtataaa aagttaaagt tattgataga taataatcct aatgaaaatt
      421 tatataaaaa tttacttgaa taaaaaaata aagttactta aaaataagca actttatctt
      481 tagtgtttat gtatttaaag gcaaaaagca agaaacttac agctaatatt attatacttt
      541 aaatatcaaa attaataaag ccagttaata aataactggc tacctagata gattcaag
//
LOCUS       DAEVGI010000376          593 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEVGI010000376 DAEVGI010000000
VERSION     DAEVGI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.378"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(175..537)
                     /locus_tag="K8M77_002667"
     CDS             complement(175..537)
                     /locus_tag="K8M77_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBI7087082.1"
                     /translation="MSCLGNLIWMIFGGFFNALGWLFWGCIWCITIVGIPVGLQCFKL
                     AGLQLAPFGKEVVTVDEGAGNLILNILWIVFGGFTLALGNLISAAMFAITIIGIPFAI
                     QSLKLAKLSLMPFGKVVR"
BASE COUNT          222 a          109 c           49 g          213 t
ORIGIN      
        1 tactcgtaat atttgtaatt ttataaaaaa ttatttttat ctataccctt ctctcaataa
       61 aatactgctt agtattaaga ataaattttc taacaaatta acaacttcga agcctttcac
      121 tatattaatc attttaagga aatatttcat aatatatttc ttataaaagg catattatct
      181 taccacttta ccaaaaggca taagagataa tttagccaat tttagtgact gtattgcaaa
      241 aggtattcct attattgtaa tagcaaacat agctgcactt attaaatttc ctagagctaa
      301 tgtaaatccc ccaaaaacta tccataatat attaagaata agatttcctg ctccctcgtc
      361 aacagttact acttctttac caaaaggagc taattgtaat cctgctaatt taaaacattg
      421 taatccaaca ggtattccta ctattgttat gcaccatata catccccaaa ataaccatcc
      481 taaagcatta aaaaatccac caaatatcat ccaaataaga tttcctagac aactcactat
      541 ttttcctcct tgtataataa atatttaatt tttataataa tatttacttt gat
//
LOCUS       DAEVGI010000377          588 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEVGI010000377 DAEVGI010000000
VERSION     DAEVGI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.379"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            127..>588
                     /locus_tag="K8M77_002668"
     CDS             127..>588
                     /locus_tag="K8M77_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087083.1"
                     /translation="MSNNKISFKNMNNSDKVKLINNSLVLDGISLKELSKKIDIQKKD
                     IIKFMNEEGFFFDSTDGYFKKDKEESTSIDIKPSVETSKSSLSKKSEREDVQSIIDSI
                     NEKLNIKDIETTNILLPEIKTSEKVSVAKEENLKDNTKTSQNITLEIPRVSR"
BASE COUNT          253 a           59 c           77 g          199 t
ORIGIN      
        1 tataccaatt gtaatatatt tcaaattgtt tgtttttatt ttataatatt tttaggaact
       61 tttgtaatat ggcaaagctc acattactat taacataatt tatatttgta ttaagggtga
      121 gaaattatga gtaacaacaa aataagcttt aaaaatatga acaattcaga taaggttaaa
      181 ttaataaata actctttagt tttagatgga atttctctta aagagctttc aaaaaaaata
      241 gatatacaaa agaaagatat aataaaattt atgaatgaag aaggtttttt ctttgatagt
      301 acagatggtt attttaaaaa ggataaagaa gaatctactt caatagatat aaagccttca
      361 gttgaaacaa gtaaatcctc cctttctaag aaatcagaac gcgaggatgt tcaatctata
      421 atagattcta taaatgaaaa attaaacata aaagatattg aaactactaa tatattatta
      481 cctgaaatta aaacatctga aaaagttagt gttgctaagg aagaaaattt aaaggataat
      541 actaagactt ctcaaaatat aactttagaa attcctagag tatctaga
//
LOCUS       DAEVGI010000378          580 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEVGI010000378 DAEVGI010000000
VERSION     DAEVGI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.380"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            39..>580
                     /locus_tag="K8M77_002669"
     CDS             39..>580
                     /locus_tag="K8M77_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087084.1"
                     /translation="MNDLLNIFIYIDENLIKNLSSVYLNGYIDIRTFKKIYDNTLSGK
                     IQLDENNKTFCSDGKSRIYNKGFKTSNRSNDFNETNYYGNDKSIENRLVGRTEEEIKR
                     IYTSFEIHNTMLKRMTTSKVIKDLENSHLVDSHISEGDFIRTKGCITETSLSSYLDSI
                     ISLIECFPLDILESLLKDKN"
BASE COUNT          252 a           62 c           73 g          193 t
ORIGIN      
        1 aaattataat ataatcaatt tagcttagga gaataataat gaatgatctt ttaaatattt
       61 ttatatatat agatgaaaat ttaataaaaa atctatcttc tgtatatcta aatggatata
      121 tagatatacg tacttttaaa aaaatttatg acaacacctt atctggaaaa attcaattag
      181 acgaaaataa taaaaccttt tgtagtgatg gaaaaagtcg aatatataat aagggtttta
      241 aaactagtaa tagatctaat gactttaatg aaacaaatta ttatggaaat gataaaagca
      301 tagaaaatag actcgttgga agaactgaag aagaaataaa aagaatttat acctcttttg
      361 aaatacacaa tactatgctt aaaagaatga ctacatcaaa agttattaag gatcttgaga
      421 actcacattt agttgattct catatttctg aaggagattt tataagaaca aaaggatgca
      481 taacagaaac ttcactctct tcttacttag attcaataat aagcttaata gaatgctttc
      541 ccttagatat attagaatct cttttaaaag ataaaaattt
//
LOCUS       DAEVGI010000379          580 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEVGI010000379 DAEVGI010000000
VERSION     DAEVGI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.381"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            136..501
                     /locus_tag="K8M77_002670"
                     /pseudo
     CDS             136..501
                     /locus_tag="K8M77_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          248 a           36 c           89 g          207 t
ORIGIN      
        1 ttatactaaa agaagtaaac tatacataaa atatttaatg tttaagaatg ggaaatattc
       61 tgaattatgg gaaggatatt tagacaaaga aagaatatta aataatggtt atattattta
      121 atattaaaca ttaatttgtt tttcacaatt tttaatgttt ataactatta taatttaaaa
      181 aatatagttg aacattcaaa tatgagcaag tacatcaaag attttaagtt atcaggtacc
      241 gattattgtg tcgtaacaat taatggagat gaagagttta aaaatttaga taataaaaca
      301 aaattagcat atatggttga tttgaataca agttgtacta tagtaatagg cgatatactt
      361 tttgatggat gtataccata taatatttat catgaaagta aagttatagt aattgttaat
      421 gataatactt atattttacc taatgaagaa aaaagtaatg aaagtaaatt gtatattaat
      481 ggtgagttgg tttatgaaga aagtttagaa gaaacttttc gtaatataaa gaaaataaaa
      541 aaaaggggct gtatcaaaat aaattttgat attagtcgtg
//
LOCUS       DAEVGI010000380          577 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEVGI010000380 DAEVGI010000000
VERSION     DAEVGI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.382"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..577
                     /locus_tag="K8M77_002671"
     CDS             <1..577
                     /locus_tag="K8M77_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087085.1"
                     /translation="YILLAGYLIYGIINKDLILLYSTGLTFITLILPRLICKALKIKL
                     SPFLNFIIVGFIFISMFLAKVNNFYAIPHWDTFLHTVSGILTFILGYMLFLCLNNYKT
                     DNVNSLVIAIFSMIFGIACTAVWEMWEFFTDQTFGLTAQLSLFDTMKDIITGSIFPII
                     LAPSLNRFLKGKKNRLFEDLTNFMKKNNKRK"
BASE COUNT          208 a           64 c           79 g          226 t
ORIGIN      
        1 ttatatttta cttgcaggat atttaatata tggaataata aataaagatt tgattttatt
       61 atattcaaca ggattaacct ttataacctt aattttacct agacttatat gtaaggcttt
      121 aaaaataaaa ttatctccat ttttaaactt tataattgta ggctttatat ttatttcaat
      181 gtttttagct aaggtaaata acttttatgc aataccacac tgggacactt ttttacatac
      241 agtatcaggg atattaacct ttatacttgg ttatatgtta tttttatgtc ttaacaatta
      301 taaaactgat aatgtaaatt ctttagttat agctattttt tctatgatat ttggaattgc
      361 ttgcacagca gtatgggaaa tgtgggagtt ctttactgat caaacctttg gattaactgc
      421 tcaattaagt ttatttgaca ctatgaaaga tataataacg ggaagcatat tccctataat
      481 tttagcgcca tctttaaata ggtttttaaa ggggaaaaag aatagattat ttgaagattt
      541 aacaaatttt atgaaaaaaa ataacaagag aaaatag
//
LOCUS       DAEVGI010000381          570 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEVGI010000381 DAEVGI010000000
VERSION     DAEVGI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.383"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..539
                     /locus_tag="K8M77_002672"
     CDS             <1..539
                     /locus_tag="K8M77_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBI7087086.1"
                     /translation="NYIPFNEQEEMDKELFLRCLNDFHDILTRDNKIAHLTSSAFAVN
                     KERNKFLMIHHNIYNSWAWTGGHSDNEKDQLKVAMKELKEETGVKNPTPLLDKAFALD
                     VLTVNGHIKRGKYVSSHLHLNLTYLIECSEEETLMLKEDENSGVIWIPFNEISKYCSE
                     PHMIPIYEKLIEKLKSLK"
BASE COUNT          232 a           68 c           85 g          185 t
ORIGIN      
        1 aaaattatat tccttttaac gaacaggaag aaatggataa agaactattt ttaagatgct
       61 taaatgattt tcatgatatt ttaacaagag ataataaaat tgctcattta actagctcag
      121 cttttgctgt aaataaagaa agaaataaat ttttaatgat tcatcataac atatataatt
      181 cttgggcatg gactggtggt cattcagata atgaaaaaga tcaactgaaa gttgcaatga
      241 aggaattaaa agaagaaact ggagttaaaa atcctactcc tcttttagat aaagcttttg
      301 cattagatgt gttaacagta aatggccaca taaaaagagg aaaatatgtt tcatcccatc
      361 ttcatttaaa tcttacttat ctaatagaat gctcagaaga ggaaactctt atgcttaaag
      421 aggatgaaaa tagcggtgtt atatggattc cttttaatga aataagtaaa tattgcagtg
      481 agcctcatat gattccaatt tatgaaaaat taatagaaaa actaaaatct ctaaaataaa
      541 ccttttataa atttaaagct aagttttata
//
LOCUS       DAEVGI010000382          566 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEVGI010000382 DAEVGI010000000
VERSION     DAEVGI010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.384"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          254 a           36 c           56 g          220 t
ORIGIN      
        1 atctaaaaaa tattgatagt attacaaaaa acacaataaa gatctttaaa aaaatgttgt
       61 tttgggaaaa atgttattta taaaagaaac attatcataa aaagaaaaaa aaacaattgg
      121 tataaatata atagtaaata aaaaaatagt cctaaaccat tgatttatta ttttttttga
      181 aggtaatgtt aatattttat ctaaatatgt gtttttatta ttcatataaa tcagtccttt
      241 ttatttttag ttaaaatata ttaaatattc agtaccagtc tgcttgttta gtaagtaaag
      301 gttaaattgc ttaagagtat taatatcata attaacattg caacttaaaa attctgtata
      361 tttagattta ggattgaaat ttttatttct agataaattt tttaaataaa agaaccttca
      421 aataaaagtt ttgaattaat gttaacaata atttaaatta tatataaata atttaaatta
      481 ttcaattatt tatagaaatt aataatgagg catatggaat taaataacaa taattaattt
      541 taataaaaaa gtagagttaa atgata
//
LOCUS       DAEVGI010000383          563 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEVGI010000383 DAEVGI010000000
VERSION     DAEVGI010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.385"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(164..520)
                     /locus_tag="K8M77_002673"
     CDS             complement(164..520)
                     /locus_tag="K8M77_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBI7087087.1"
                     /translation="MSCLFLEYPKCSTCRKAKKWLEENNIDFTDRMIVEENPTKEELK
                     AWYEKSGVKLNKFFNTSGKLYREMNLKDKVKTASEDELLDILSSNGMLVKRPLLIKED
                     LVLIGFKEEEWAENLK"
BASE COUNT          189 a          103 c           46 g          225 t
ORIGIN      
        1 atagcttaaa accagataaa taccagaatt tagaagaatg tgactattct tctatttgtc
       61 atgtcctttt ttattaaaat tagtaagaat ttttattcaa cagtcaaaaa agaaggcgta
      121 atcaaaatga ttaaattaat cattttgata cacccccttt ttgttatttt aaattttcag
      181 cccattcttc ttccttaaat cctataagaa ctaaatcctc ttttataagc aaaggtcttt
      241 ttactaacat accattactt gataagatat ctaaaagttc atcttcacta gctgttttta
      301 ctttatcctt aagattcatc tctctataaa gtttacctga agtattaaag aacttattta
      361 atttaactcc actcttctca taccaagcct ttaattcttc tttagtagga ttctcttcca
      421 caatcattct atctgtaaaa tctatattgt tttcttctaa ccatttctta gcttttctac
      481 aagtactgca tttaggatat tctaaaaata aacaactcat tttcttacac cttctttaaa
      541 atcatgatat ttatacaata aaa
//
LOCUS       DAEVGI010000384          563 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEVGI010000384 DAEVGI010000000
VERSION     DAEVGI010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.386"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..338)
                     /locus_tag="K8M77_002674"
                     /pseudo
     CDS             complement(1..338)
                     /locus_tag="K8M77_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477043.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          195 a           88 c           41 g          239 t
ORIGIN      
        1 gctctccctt cttttatatt tttatttctt atttttaaac cttcatattt ccttgaaagt
       61 tttctttgct ctctttttaa tttcttttct acttttttaa cagtagatgt tttatttata
      121 tttttaaatt tattactatt tgaacatatt gcaaattcct taatacctag gtcaattcct
      181 aagccttcat tatttggatt aaatattttc atacctgtat tgccattaga tatttttata
      241 cctatattac tattagatat ttttatatct atttattcaa ccaatataga tacataatat
      301 ctatcagctt tttgacttac tgtaccactt ttgactattg aatctacagg atctccttaa
      361 aacaataaat tctaaaatcc tcctaattaa gatgttttaa ataactaaat tctaacacac
      421 aattttagct ttaaaatcat atatttaaag ttaaaaataa ttacaaataa aaaaccgtag
      481 aaaattctac ggttaacaat caaaacaaat ataataatac ttaaaacacc atagttttat
      541 ttaaagacag gaggttttcg aac
//
LOCUS       DAEVGI010000385          545 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEVGI010000385 DAEVGI010000000
VERSION     DAEVGI010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.387"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            75..434
                     /locus_tag="K8M77_002675"
     CDS             75..434
                     /locus_tag="K8M77_002675"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBI7087088.1"
                     /translation="MKIALIAHDKKKEEMIELAKDFEDKLSKHILVATGTTGLKIMQN
                     TSLEVKRCKSGPLGGDQEIGAMVANHDVDMVIFLRDPLTAQPHEPDISALLRLCDVYK
                     VPLATNTESAKLIMADI"
BASE COUNT          226 a           59 c           94 g          166 t
ORIGIN      
        1 taaatattaa aaaataaata aatttttatc caatggacat agttaatttg ttcgatgaaa
       61 aaggggagag ttaaatgaaa atagcattaa tagcacatga taagaaaaaa gaagaaatga
      121 tagagcttgc aaaagatttt gaagataagt taagtaaaca tatacttgtt gcaacgggta
      181 ctacaggact taaaattatg caaaatacat ctttagaagt taaaagatgt aaaagtggcc
      241 cactaggggg agatcaagaa ataggagcaa tggtagcaaa ccacgatgtt gatatggtaa
      301 ttttcttaag agatccatta acagctcaac ctcatgaacc agatattagt gcattactta
      361 gactttgtga tgtttataag gttccactag ctacaaatac agaaagcgct aagcttataa
      421 tggctgatat ttaaagttaa tataataaat taagaagtta aatttgagaa tttttccgtc
      481 aaattataat gaaatagtaa ctttattttt atcaatatta gttataggat tataaaattt
      541 cagaa
//
LOCUS       DAEVGI010000386          544 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEVGI010000386 DAEVGI010000000
VERSION     DAEVGI010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.388"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(4..402)
                     /locus_tag="K8M77_002676"
     CDS             complement(4..402)
                     /locus_tag="K8M77_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBI7087089.1"
                     /translation="MPKLLIPAIIFITLALIFYTIGVWSEHRAGILKKKHVIIFWCGL
                     VCDTLGTYTMSRIVASGTDKGITPTDLLIHQSTGMLAIILMLIHAVWATIVLNRGSEK
                     AKAIFHKFSLVVWFIWLIPYFVGMYIGMTS"
BASE COUNT          194 a           84 c           72 g          194 t
ORIGIN      
        1 ttattatgat gtcattccga tatacatacc tacaaagtat gggataagcc atatgaacca
       61 aactactaaa ctgaatttgt ggaatatagc ttttgctttt tcactacctc tgtttaaaac
      121 aattgttgcc caaacagcat gtataagcat taaaataata gcaagcatac ctgttgattg
      181 atgtattaat aaatcagttg gagtaatacc tttgtcagtt ccagatgcaa cgattctact
      241 cattgtataa gttccaagag tatcgcatac taaaccacac caaaagatta ttacatgttt
      301 tttctttaat atacctgctc tatgctcgct ccaaacacca attgtataaa atattagtgc
      361 taatgttata aatataatag ctggaattaa taatttaggc ataaaaaaac cttctttctt
      421 aaattagaat aaaattttat agcctgtcct taagttgaag ttttcatata ttaaaagaaa
      481 tctaaagttt ggatttactt taatatgtga aaattaattt tcattgtgga ctaggttcat
      541 atta
//
LOCUS       DAEVGI010000387          543 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEVGI010000387 DAEVGI010000000
VERSION     DAEVGI010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.389"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..>543)
                     /locus_tag="K8M77_002677"
     CDS             complement(<1..>543)
                     /locus_tag="K8M77_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI7087090.1"
                     /translation="NVNPGDLVGSTPTFPINAVVKGDFLYLRDANGNQILGITVSDGD
                     EITVLDIGYSKQLVLVQYPTSSGYRQGYVTNATSIIKYKDDYSWVNGSTPEAVYDLDK
                     TTQIGTLDPRERAVVLYKVDGMTAVAYDTGKGKLTKSGLVHYEGSGSSNGGGSINGIA
                     PGEVVPGGFTYENNAQVVGD"
BASE COUNT          152 a          117 c           61 g          213 t
ORIGIN      
        1 tcatctccta caacttgtgc attattttca tatgtgaatc cacctggtac aacttctcct
       61 ggtgcaattc catttatact tccaccacca ttagaactac ctgaaccttc ataatgaact
      121 aatcctgatt ttgtaagctt accttttcct gtatcataag ctactgctgt cattccatct
      181 actttatata aaactacggc tctttctctt ggatctaaag ttcctatttg agttgtctta
      241 tctaaatcat atacagcctc aggtgttgaa ccatttaccc atgaataatc atctttatat
      301 tttattatac ttgtcgcatt ggttacatat ccttgtctat atccagaact tgttggatat
      361 tgaactaata ctaattgttt tgagtaacct atatctaaaa ctgtaatttc atctccatca
      421 cttactgtta ttcctaatat ttgattacca tttgcatctc ttagatataa aaaatctcct
      481 ttgactactg catttattgg aaatgtagga gtacttccta ctaaatctcc tggattaaca
      541 ttt
//
LOCUS       DAEVGI010000388          542 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEVGI010000388 DAEVGI010000000
VERSION     DAEVGI010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.390"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..191)
                     /locus_tag="K8M77_002678"
     CDS             complement(<1..191)
                     /locus_tag="K8M77_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBI7087091.1"
                     /translation="MQELYEKIKLCKLGNKKVLQEVVNSFEKTIMIELFKFKKENSDI
                     FMNEYDFQDKKSEITLNVL"
     gene            429..>542
                     /locus_tag="K8M77_002679"
     CDS             429..>542
                     /locus_tag="K8M77_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7087092.1"
                     /translation="MKIAIRGGHNFLSKGACGLIDETTENRNVYKAVIKNLI"
BASE COUNT          207 a           69 c           52 g          214 t
ORIGIN      
        1 tttagaacat ttaatgtaat ttcactcttt ttatcctgaa agtcatattc attcataaaa
       61 atatcgctat tttctttctt aaatttaaat aattctatca ttatagtttt ctcaaagcta
      121 ttaaccactt cttgtaaaac ctttttgttt cctaatttac acaactttat tttttcatat
      181 aattcttgca taaaattctc ctcaaaatct cttttatttt ttaaagagaa aaaatgagga
      241 aaagtgtaaa tctcaaaaat aagcttaaaa aaaatgtaac gttccgttct ataattttac
      301 ataatataca tttttgtttc aaaagaccta atatatttta tttttatatt tacaaaataa
      361 acgcctatca ctcttttaaa tatataagct tatttaagca atatttttaa aaggaaagga
      421 agaaaaatat gaaaattgct atacgtggtg gtcataactt tttatcaaag ggagcttgtg
      481 gattaataga tgaaactaca gaaaatagaa atgtttataa agctgttatt aaaaacttaa
      541 tt
//
LOCUS       DAEVGI010000389          513 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEVGI010000389 DAEVGI010000000
VERSION     DAEVGI010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.391"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(271..480)
                     /locus_tag="K8M77_002680"
     CDS             complement(271..480)
                     /locus_tag="K8M77_002680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circular bacteriocin, circularin A/uberolysin
                     family"
                     /protein_id="HBI7087093.1"
                     /translation="MIMKVAALLGISSNWAMKIVDLIATGASIWAIAAAVAASGGILA
                     IGIVVLRELVMKKIKQVGYAAAVTY"
BASE COUNT          189 a           84 c           47 g          193 t
ORIGIN      
        1 taaaaaataa aaatttataa ttgttatagc tattattgat atactcccta aaatagaagt
       61 taaaagcata aattttctat caattacaat atttaagtct gggattttta atattttcag
      121 aatcgttgaa aaatatattg ctaaaaaaaa agttaaagaa ctaaatacaa ataaataaaa
      181 gcctatattg gattttaatt ttttcatctt catacctttc atataaaaat tttataaaat
      241 ggctgttaaa ttaagccctt ttattgataa ttaataagtt actgctgctg cataacctac
      301 ctgtttaatt tttttcatta ctagttctct taatacaact ataccaatgg caagtattcc
      361 accacttgct gctactgcag cagctattgc ccatatactt gctcctgtag caataagatc
      421 tacaattttc attgcccaat tactactaat tcctagtaac gcagctactt tcataatcat
      481 aaaacattac ctcctataaa ttttattttt taa
//
LOCUS       DAEVGI010000390          511 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEVGI010000390 DAEVGI010000000
VERSION     DAEVGI010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.392"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..474
                     /locus_tag="K8M77_002681"
     CDS             <1..474
                     /locus_tag="K8M77_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HBI7087094.1"
                     /translation="NKKQIEQSIEKCSVKAPSDGKLNINANLEQGIMLQTGAVVASII
                     PNSDTYKVELMIPTKDIANIKVSEDVKYSFEALPYREYGFLDGKIESLSPDSKVDSEK
                     GISFFTWEGSLSSNSLYSNKGEESFIKPGMMCEAKIITRKEKMLYYLLEKIGFKN"
BASE COUNT          224 a           49 c           89 g          149 t
ORIGIN      
        1 aataagaaac aaatagaaca aagcattgaa aagtgtagtg ttaaagctcc atcagatgga
       61 aagttaaata taaatgccaa tttagaacag ggtataatgt tacaaacagg agctgtagtt
      121 gcaagtatta ttccaaattc agatacttat aaagttgaat taatgatacc aacaaaggat
      181 atagcaaata ttaaagtttc tgaagatgtt aaatatagct ttgaagcatt accatataga
      241 gaatatggat tcttagatgg caaaatagaa agtttaagcc ctgattcaaa ggtagattct
      301 gaaaaaggaa tatctttctt cacatgggaa ggaagtttaa gttctaatag tctttatagt
      361 aataaaggtg aagaaagttt tattaaacct ggaatgatgt gtgaagctaa aataataaca
      421 agaaaagaaa agatgttata ttatctctta gaaaaaatag gttttaaaaa ctaattaata
      481 taaatataaa aaggagaata caatagcttt a
//
LOCUS       DAEVGI010000391          507 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEVGI010000391 DAEVGI010000000
VERSION     DAEVGI010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.393"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            14..430
                     /locus_tag="K8M77_002682"
     CDS             14..430
                     /locus_tag="K8M77_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009172226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI7087095.1"
                     /translation="MLKLENQLCFRLYASSREVIKKYKPILDQYNLTYTQYLTMLVLW
                     ENEKITVKGIGEKLHLDSGTLTPLIKKLENMNLVTKYRSKEDDRVVIVELTDKGRNLK
                     DDMIDVPRKAFCSLGIEKEQILELKKNLDSLLEILK"
BASE COUNT          227 a           45 c           76 g          159 t
ORIGIN      
        1 ggataaatat aatattttaa aattagaaaa ccaactttgt tttagattgt atgcttcttc
       61 aagagaagtt ataaagaaat ataaacctat tttagatcaa tataatttaa cttacacaca
      121 atatttaaca atgcttgttt tgtgggaaaa tgaaaaaata acagttaaag ggattggtga
      181 aaaactacat cttgattctg gaactttaac acctcttata aaaaagttag aaaatatgaa
      241 tcttgtaaca aaatatagat caaaagagga tgatagagtt gtaatagttg aattaacaga
      301 taaggggaga aatttaaaag atgatatgat agatgtacca agaaaggcat tttgttcttt
      361 aggaatagaa aaagagcaaa tattagaact taagaaaaat ttagactcat tactagaaat
      421 attaaaataa aaagggcatg tatcaaaata acttattaat aaactttaca aacagtatga
      481 atataatatc tagattagtt ttagaat
//
LOCUS       DAEVGI010000392          503 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEVGI010000392 DAEVGI010000000
VERSION     DAEVGI010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.394"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            51..470
                     /locus_tag="K8M77_002683"
     CDS             51..470
                     /locus_tag="K8M77_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBI7087096.1"
                     /translation="MDNITTIFKEKKLKLTPQRIAVYKYLKSTKEHPSAETIYKALQE
                     DYPTMSLATVYKALKTLVEVELIQEINVGEGNFRYDGNSNDHCHVQCLACGKVDDLEN
                     LDYNHLNEEAQKHSNYRILSNKMYFYGICEECQNKHE"
BASE COUNT          208 a           61 c           74 g          160 t
ORIGIN      
        1 taattattat ataatatact taattcttat atatgatagg gatggtggaa atggacaata
       61 taactactat ttttaaagaa aaaaagttaa aactaactcc tcaaaggatt gctgtttata
      121 aatacttaaa aagtacaaaa gaacacccat ctgctgagac tatttacaaa gctttacagg
      181 aagattaccc tacaatgtca ttagctacag tttataaagc attaaaaact cttgtagaag
      241 tggaattaat acaagaaatt aatgttggtg aaggaaactt tagatatgat ggtaactcaa
      301 atgatcactg tcatgtccaa tgtttagctt gtggtaaagt tgatgatctt gaaaatttag
      361 attataatca tctaaatgaa gaagcacaaa aacattcaaa ttatagaata ctttcaaaca
      421 aaatgtattt ctatggaata tgcgaagaat gtcaaaataa acatgaataa aaaagagtgg
      481 taatttacca ctctttttta tat
//
LOCUS       DAEVGI010000393          485 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEVGI010000393 DAEVGI010000000
VERSION     DAEVGI010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.395"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            164..>485
                     /locus_tag="K8M77_002684"
     CDS             164..>485
                     /locus_tag="K8M77_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBI7087097.1"
                     /translation="MVIIFMGIVMFIIGLICKKLHDDYEEYPDIFVGYKLKYAMRNEE
                     TWEEANNYILKPCIFVFIFSIIFTLIIRFFNLYISGLEYFLVGMFIISIPVICTEIHI
                     RRFNK"
BASE COUNT          184 a           27 c           84 g          190 t
ORIGIN      
        1 agaaatataa aaaaaagtaa tggaaatgaa tgtatttaaa agataatgaa cgaatttaaa
       61 agaatatata gaatgaaaaa tgtggtaaat agtggtatta tgttgatgat tgtaatttta
      121 cattttttag tattggtatc tgataagaaa ggggaaaata tttttggtta taatttttat
      181 gggaatagtt atgtttatta ttgggcttat ttgtaaaaag cttcatgatg actatgagga
      241 atatcccgat atttttgtag ggtataaatt aaaatatgca atgagaaatg aagaaacgtg
      301 ggaagaagct aacaattata ttttaaagcc atgtatcttt gtatttatat tctcaataat
      361 attcactttg ataattagat tttttaattt atatatttct ggtttggaat attttttagt
      421 tgggatgttt attataagta ttcctgtaat ttgtactgaa attcatataa gaagattcaa
      481 taaaa
//
LOCUS       DAEVGI010000394          479 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEVGI010000394 DAEVGI010000000
VERSION     DAEVGI010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.396"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            264..>479
                     /locus_tag="K8M77_002685"
     CDS             264..>479
                     /locus_tag="K8M77_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI7087098.1"
                     /translation="MLHKGNEKIKITFNEHFLSRVDFMRAVVRYLIKNNRDCRVIDFY
                     HLIVDDKKYYLRDQNLTPEGAKCIQNAI"
BASE COUNT          195 a           36 c           73 g          175 t
ORIGIN      
        1 tataataaaa gtgtaaagag aaagaggtat tactacaaaa taaggtttaa aaattttaga
       61 atataaacct aaaatggatt attttaaaac ttgtaaacgt ttatagagtc ctggattttg
      121 aattttgctt tttaattaac agtgatctaa ttctcttttc ataagatata gctttaatga
      181 aaatttatgt gtttttagga ctttacatac taatatgatt cattttataa ggaatttatg
      241 attattaaaa ggggtgttgt aatatgttac acaaaggcaa tgaaaaaata aaaataactt
      301 ttaatgaaca ttttttaagt agagtcgatt ttatgagagc agtggttaga tatttaatta
      361 aaaacaatag agattgtaga gtaatagatt tttatcattt aatagtagat gataaaaaat
      421 attatttaag ggatcaaaat ttaacacctg aaggtgcaaa atgtattcaa aatgcaatc
//
LOCUS       DAEVGI010000395          474 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEVGI010000395 DAEVGI010000000
VERSION     DAEVGI010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.397"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            218..433
                     /locus_tag="K8M77_002686"
     CDS             218..433
                     /locus_tag="K8M77_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003449914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087099.1"
                     /translation="MSDNKLKEDLVKVYKDWKDLEKKAGKKIKHHHELKKEEKEAEIQ
                     RFSDYAGLSVPITEEMLLYLDEEYFRV"
BASE COUNT          219 a           46 c           67 g          142 t
ORIGIN      
        1 tattagatct ctatagtaaa tagagagttt ctgaaaaaag cttttttgaa aaattaaaat
       61 taaatcatga ttttaaaaag ttagaaaaaa aagcaaataa aacttataac aaagaaattt
      121 tctgaattcg ctaaaattga tacaccattg acagaaaaag aaatacttga attagaagaa
      181 tattataata atacatttat ttaaggagat gtttttcatg agcgataata agttaaaaga
      241 agatttagta aaagtttata aagattggaa agaccttgaa aagaaagctg gcaaaaaaat
      301 taaacatcat cacgagctaa aaaaagaaga gaaagaagct gagatccaaa gattttcaga
      361 ctatgctgga ttgtcagttc ctataactga agaaatgctt ctatacttag atgaagaata
      421 ttttagagtt taaaaaatac ttacccttaa ctaaaataaa atttttttaa aata
//
LOCUS       DAEVGI010000396          472 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEVGI010000396 DAEVGI010000000
VERSION     DAEVGI010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.398"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>472
                     /locus_tag="K8M77_002687"
     CDS             <1..>472
                     /locus_tag="K8M77_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7087100.1"
                     /translation="WIINAVPSKALPLWKAVQEYLYNQLKYFGADRQALDATRILRVP
                     GSINSKSKTVVNILDEYEYIYDLREIQNGFLPELKPYEKKKGRPSKINYIYRERSLYY
                     GRIQDIIKLCELREYDLKGHRELILFLYRYYLCSFTEDIEKALNDVLELNSMFRQ"
BASE COUNT          206 a           45 c           78 g          143 t
ORIGIN      
        1 tggataataa atgcagtacc tagtaaagca ttaccattgt ggaaagctgt tcaagaatat
       61 ttatataatc aattaaaata ttttggagca gatagacaag ccttagatgc aacaagaata
      121 ttaagagttc caggaagtat aaactctaaa tcaaaaacag tagttaatat attagatgag
      181 tacgaatata tttatgactt aagagaaata caaaatggat ttttacctga attaaaacca
      241 tatgaaaaga aaaaaggcag accaagtaaa ataaattata tttatagaga aagaagttta
      301 tattatggaa ggatacaaga cataataaaa ctttgtgaat taagagaata tgatttaaaa
      361 ggacatagag agcttatatt atttttatat agatattacc tttgtagctt tacagaagac
      421 attgagaagg cattaaatga tgttttagaa cttaatagta tgtttagaca ac
//
LOCUS       DAEVGI010000397          458 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEVGI010000397 DAEVGI010000000
VERSION     DAEVGI010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.400"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(120..389)
                     /locus_tag="K8M77_002688"
     CDS             complement(120..389)
                     /locus_tag="K8M77_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087101.1"
                     /translation="MINRDFIISFIVVTCIIYLIYVCYGKYLEKKQIYLKTLTVFLII
                     EVLRRIFIYGKVNNRLISGLSFIQLIFLVISVIMYGLLYFKNNLS"
BASE COUNT          180 a           66 c           38 g          174 t
ORIGIN      
        1 aagtgttata tggtttatgc ttttagcata tgtcgtggca atccatccaa tgattgaaat
       61 tacgggtgtt cttgccaaag gcataaaaaa aatagtaaag tttattctta tgattattat
      121 tagcttaaat tattcttaaa atacaaaagt ccatacataa ttactgaaat caccaaaaat
      181 attaattgaa taaaagaaag tccacttatt aaacgattat taacttttcc ataaataaaa
      241 attcttctta agacttctat tattaaaaat acagttaaag ttttaagata tatttgcttt
      301 ttctctaaat atttaccgta acacacatat ataagatata taatacaagt tacaacaata
      361 aagcttataa taaaatctct atttatcatc ctatcccccc tcactcaaac tatattttta
      421 tttaatttta caattctata tttttatttt acaataaa
//
LOCUS       DAEVGI010000398          458 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEVGI010000398 DAEVGI010000000
VERSION     DAEVGI010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.401"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          195 a           49 c           25 g          189 t
ORIGIN      
        1 taaacataaa atttttaaaa aactaaattt atatataaaa aaacaaataa aaaaaaccta
       61 accaatttta aaaatttaaa actaaataat tagtaagtgg ctataccact ataaaaagac
      121 tcattaaact ccagtaaaaa ctactggcca atactaatat ttgttttatt ctttaaaatt
      181 tttagattta tctatttgtt attaatctat gcttataaaa tataagctaa tatttatttt
      241 tttatgttaa tcttagtctt taaaagaccc ttttaaaatt taagtaatcc taagctttag
      301 cttaaaaaaa tttaagttat cttaaaccta tagtttaaaa aatttgaata taagctaaaa
      361 actttttcaa cttatattgt aataatctat taatttaatt ttataacatt ttaaaagttt
      421 aatcaaattc ttaacttatt tattatttta attttttc
//
LOCUS       DAEVGI010000399          455 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEVGI010000399 DAEVGI010000000
VERSION     DAEVGI010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.402"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            145..>455
                     /locus_tag="K8M77_002689"
     CDS             145..>455
                     /locus_tag="K8M77_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBI7087102.1"
                     /translation="MKKFVCTVCGYVYEGEKAPEKCPVCGVGADKFVEQSGDLAFADE
                     HRIGVAKGVDERIIEGLQANFTGECTEVGMYLAMSRQADREGFPEVAEAYKRIAFEEA
                     E"
BASE COUNT          183 a           43 c          100 g          129 t
ORIGIN      
        1 ttattattat ataatattga taaaggttaa gaaaaaatta acagattaaa tctatagatt
       61 ataaagaaag agatttagta ggataatttt cttaataacc tatggatgaa aattatggag
      121 acataatatt aggaggattt tattatgaaa aaatttgttt gtacagtatg tggatatgtt
      181 tatgaaggag aaaaagcacc agaaaaatgc ccagtatgtg gagttggagc tgataaattc
      241 gtagaacaaa gtggagattt agctttcgct gatgaacata gaatcggagt tgctaaaggt
      301 gtagatgaaa gaataatcga aggattacaa gctaacttta ctggtgaatg cacagaagta
      361 ggaatgtact tagctatgtc aagacaagct gatagagaag gattcccaga agttgcagaa
      421 gcttataaaa gaatagcttt cgaagaagca gaaca
//
LOCUS       DAEVGI010000400          450 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEVGI010000400 DAEVGI010000000
VERSION     DAEVGI010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.403"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..415
                     /locus_tag="K8M77_002690"
     CDS             <1..415
                     /locus_tag="K8M77_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087103.1"
                     /translation="LNDLLNLDLNKCSIFDITEEHLILLKTKDFHTQNNFYFYLYNKL
                     TSIEKTKRKELAYCNYLISYYLFIVMTPLYYEELAFYHGKKAFQLENSTKYMEWLLLF
                     GTLEKPLLTYEICSNLAKEILKENPNSTLANFFLM"
BASE COUNT          176 a           60 c           46 g          168 t
ORIGIN      
        1 attaaatgac ttattgaatt tagatttaaa taaatgtagt atttttgaca taacagaaga
       61 acacttaatc ttacttaaaa caaaggattt tcacactcaa aataattttt atttttatct
      121 ttataataaa cttacttcta tagaaaagac aaaaagaaag gaacttgctt actgcaatta
      181 tcttatatcc tactatttat ttatagtaat gacaccctta tattatgaag aattagcttt
      241 ttatcatgga aaaaaagcct ttcagttaga aaattccaca aaatatatgg aatggctttt
      301 attatttgga accttagaga agcctctatt aacttatgag atttgttcta atcttgctaa
      361 agaaatttta aaagaaaatc caaattcaac cttagctaac ttctttctta tgtaagatat
      421 gaaaaaataa ctaataattt tcctcttatt
//
LOCUS       DAEVGI010000401          448 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEVGI010000401 DAEVGI010000000
VERSION     DAEVGI010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.404"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..340)
                     /locus_tag="K8M77_002691"
     CDS             complement(<1..340)
                     /locus_tag="K8M77_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087104.1"
                     /translation="MKYALDINDFYLRKVLIEKLKKAGNLTIDCFNEECCLGESFFKK
                     VLLSNNAKADIFIRITKSIGEDKRIEILGDDQILRRVVSLNLEDIVYYIGLSHISIKS
                     GKELYLVKNLN"
BASE COUNT          163 a           73 c           28 g          184 t
ORIGIN      
        1 aatttaaatt ttttactaaa tataattctt tcccactttt aatacttata tgtgaaagtc
       61 ctatataata aactatatcc tctaaattta aagatacaac acgtctaaga atttgatcat
      121 cacctaaaat ttctattctc ttatcctctc ctatactctt agttatcctt ataaatatat
      181 ctgcctttgc gttattagat aataaaactt tcttaaaaaa actttctcct aagcaacact
      241 cttcattaaa acaatctata gtaagatttc ctgctttctt taatttttct attaatactt
      301 ttcttaaata gaagtcattt atatctaatg catatttcat tataaatcac ctccttaatt
      361 ataagaataa ttgtaatata tattattcat tttctttaaa aaagttctac taaaaacata
      421 agtttcataa attaaactta aggaggtg
//
LOCUS       DAEVGI010000402          440 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEVGI010000402 DAEVGI010000000
VERSION     DAEVGI010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.405"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            45..143
                     /locus_tag="K8M77_002692"
                     /pseudo
     CDS             45..143
                     /locus_tag="K8M77_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
BASE COUNT          188 a           53 c           44 g          155 t
ORIGIN      
        1 aataatataa agtatataaa tcttataata aatgcataaa cattatgagt gaaaaattta
       61 atcctgatac cttagaaaaa cttaataatc cagaaaggtt aaacttaata gatttgagag
      121 aacttttaaa aattctagac ttataaatga ggattatatt atatcagata tgaaataaga
      181 tgccttttcg cctattgaag atgtgttact ttcaaatgaa ttcctttatc tattaggaga
      241 aatattaaaa aaagagcctt cctttaaccg ctctggaagg ctaaaaataa attatataac
      301 taaatctata catatataaa ctaatatctt aatacgttta cattataatt ctaattttat
      361 accaaatcaa gctttttcaa aaaaatagtt atttttatcc ataataaaga gaaaaacttt
      421 aaatatatat ttgtatccct
//
LOCUS       DAEVGI010000403          435 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEVGI010000403 DAEVGI010000000
VERSION     DAEVGI010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.406"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>435
                     /locus_tag="K8M77_002693"
     CDS             <1..>435
                     /locus_tag="K8M77_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7087105.1"
                     /translation="DVNFRWLLQGQKAPSHNTIARFKSSRMKYCLEDLFNQLVLKLNE
                     KDEIKFENLFIDGTKIEANANRYTFVWKKSTKKFEEKLQIKVRKLIENINKDLNITFH
                     NENKISVSEATNLLTYIILLKEKTNLEFVYGKGKRKSKLQKYA"
BASE COUNT          182 a           47 c           71 g          135 t
ORIGIN      
        1 gatgtgaatt ttcgttggct tttgcagggg caaaaggctc catcgcacaa tactatagct
       61 agatttaaaa gctctagaat gaaatattgt ctagaagatt tgtttaatca actggtttta
      121 aagttaaatg aaaaagatga gattaaattt gaaaatctat ttattgatgg aacaaaaatc
      181 gaagctaatg caaaccgtta tacatttgtt tggaagaaat ctacaaaaaa atttgaagag
      241 aaattacaaa ttaaagttag aaaactaatt gaaaatatta ataaggactt aaatatcact
      301 tttcataatg aaaataagat atctgtatca gaggctacta acttgctaac ttacattatt
      361 ttattaaagg agaagactaa tctagaattt gtttatggta aagggaaaag aaaaagcaaa
      421 cttcaaaaat atgct
//
LOCUS       DAEVGI010000404          416 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEVGI010000404 DAEVGI010000000
VERSION     DAEVGI010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.407"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(4..321)
                     /locus_tag="K8M77_002694"
     CDS             complement(4..321)
                     /locus_tag="K8M77_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087106.1"
                     /translation="MIIIHRDNLLDVMRSYKIIIDDTYYGKIKCGETKQINLEKGDHT
                     IYLKIDWCRSPKLNFSTSDNETIFFDCGNYMNGWKQLLFPLYITFLKNKYLFLEETNK
                     KFS"
BASE COUNT          148 a           58 c           44 g          166 t
ORIGIN      
        1 acactaggag aattttttat tagtttcttc taaaaataaa tatttattct taaggaaagt
       61 tatataaagt gggaataaaa gttgtttcca accattcata taattaccac aatcaaaaaa
      121 tatggtttca ttatctgaag tagaaaaatt caatttaggg cttctacacc aatctatttt
      181 tagataaatt gtgtgatccc ctttctctaa atttatttgt tttgtttccc cacattttat
      241 ctttccataa taggtatcat ctataattat tttataagat ctcataacgt caagtaagtt
      301 atctctatgt attattatca taaatacccc accttttatt accgtaattt atatgtatat
      361 tttactaaaa tttaaacaaa cagtaaataa aaggaggata gctttgttaa ttatgt
//
LOCUS       DAEVGI010000405          415 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEVGI010000405 DAEVGI010000000
VERSION     DAEVGI010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.408"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..171
                     /locus_tag="K8M77_002695"
     CDS             <1..171
                     /locus_tag="K8M77_002695"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7087107.1"
                     /translation="YQEQLKAQGKVTKKEKLSEIEELYRIELSPSTISYITDKVFGTA
                     TEWQNRMLNNVY"
BASE COUNT          165 a           40 c           56 g          154 t
ORIGIN      
        1 taccaagagc aattaaaagc tcaaggaaaa gtaacaaaaa aagagaaatt atcagaaata
       61 gaagaattat atagaattga actgtctcca tcaactattt cctacataac ggataaagtt
      121 ttcggaactg ctactgaatg gcaaaatcga atgcttaata atgtatatta attctagctt
      181 agtatttttg tagaatttta ttatgtaagg ataataaaat taagaagttt ttagtatgtc
      241 aagaaatttc taaatggtta ttttgagctt attttagtat atattgtgtg tacacggtta
      301 cataaaagat atataattaa tttatattca aatctattaa attataacaa gtttgatttt
      361 cttacaattt atttgaaaat actaaattaa tatacttgta tgtttgaatc tgtta
//
LOCUS       DAEVGI010000406          413 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEVGI010000406 DAEVGI010000000
VERSION     DAEVGI010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.409"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(1..411)
                     /locus_tag="K8M77_002696"
     CDS             complement(1..411)
                     /locus_tag="K8M77_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBI7087108.1"
                     /translation="MEVKEIFKANGVKVTKARLLIYDLLKESDRSVTADYIYGKCKEI
                     NLSTVYRTLEVFLEMDLVDKFELGDGKSSYKLKNRNIHKHILECDLCHKEIEVPCPMQ
                     QIEELLRNQTGFKVKEHNLTLKGICEECIDNKKK"
BASE COUNT          133 a           79 c           28 g          173 t
ORIGIN      
        1 ttattttttc ttattatcta tacactcttc acatataccc tttaaagtaa gattatgctc
       61 ttttacttta aatcctgttt gatttcttaa aagctcctca atctgttgca taggacatgg
      121 aacttctatt tctttatgac ataaatcaca ttctaaaata tgcttatgaa tatttctatt
      181 ctttaattta taagaacttt ttccatctcc taattcaaac ttatctacta aatccatctc
      241 taaaaatacc tctagagttc tatagacagt agataaattt atctctttac atttaccata
      301 tatataatcg gcagtaacac ttctatcact ttcctttaac aaatcatata ttaataatct
      361 agctttggta actttaacac cattagcttt aaatatttct tttacttcca taa
//
LOCUS       DAEVGI010000407          404 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEVGI010000407 DAEVGI010000000
VERSION     DAEVGI010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.410"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          184 a           32 c           63 g          125 t
ORIGIN      
        1 tttaaattta gttatattta gtttttaata tggaatttta taagtgaaat tttgattgta
       61 agcttaaata aatagagtat ttatatccta aatgtagtaa ttatcaagga ataggattta
      121 taaaaatgga atgtttggaa ttaaattgaa ttaaaataaa gaaaacaaaa ccaggcgtat
      181 caatataaaa aaaataaagt attagaatga aaaatagagt atttcaagaa taattgtcat
      241 aaataatgaa atatatagca agtatatatt atctaacatg gtaagataaa tctcgtcgct
      301 gagagacaca caaacaactc aacaaaacaa attaaaagtt ttgaaaaaaa gttgttgaca
      361 aagtttcaaa gtgatgataa actaaggaag tcgcttgagg gcga
//
LOCUS       DAEVGI010000408          403 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEVGI010000408 DAEVGI010000000
VERSION     DAEVGI010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.411"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          164 a           24 c           60 g          155 t
ORIGIN      
        1 ttttgtaaat tcaaaaaaat aagaacatat agaaaatggc tatttgaaag gtggtatgac
       61 caatttcaat tgtcgaaatc gaattagata atagagtgca taaataaaat gaatatttta
      121 acgaaattaa aagttagaag agaataatgt caaaaaataa tttgttactg taatatacgt
      181 aacaatttgt taattttaga acattgttaa attaaagagg tagtggtaat atttataagt
      241 aaaataagta ttaatttata ttgtatatat tgttgagtga gtgaatgaaa agctattcat
      301 tgggaggaat agcttttctt tttttagaaa atagcttata ggtttaataa tttttcttct
      361 ttaaatatta tttacttaat ttaaatttat attcaagtgt taa
//
LOCUS       DAEVGI010000409          401 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEVGI010000409 DAEVGI010000000
VERSION     DAEVGI010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.412"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..308)
                     /locus_tag="K8M77_002697"
     CDS             complement(<1..308)
                     /locus_tag="K8M77_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBI7087109.1"
                     /translation="MIKILKDILFSFYLLISSVVFSVILTLNISPVLYSLFIKLNKTP
                     NYNNLSSEEILNDYNNIIYYLNSPQEEVLKFKNFKISPIGEFHFLEVKEIFSSIYLIG
                     "
BASE COUNT          165 a           37 c           42 g          157 t
ORIGIN      
        1 aatcctatta aatatattga agaaaaaatc tctttaactt ccagaaaatg aaattctcct
       61 ataggactta ttttaaaatt tttaaactta aggacttctt cttggggaga atttaaataa
      121 taaattatat tattatagtc atttaaaatt tcttcactac taaggttgtt atagttaggt
      181 gttttattaa gcttaataaa tagggagtag agcacaggag aaatatttaa tgttaaaata
      241 acagaaaata ctactgaact tattagaagg taaaatgaaa ataatatatc ttttaaaatc
      301 ttaatcatta tgtctcctta aaataattat ttttatttat tctagacttt tttattaaat
      361 attaaacaaa aatacatatt ttaagaaaaa ttttagtatt a
//
LOCUS       DAEVGI010000410          396 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEVGI010000410 DAEVGI010000000
VERSION     DAEVGI010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.413"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          180 a           32 c           47 g          137 t
ORIGIN      
        1 aaaaaaagtt attttgatac acgcccttat atttttatta tcttaaataa gtgattaaat
       61 taatgttaca taaataaaaa atagtatttt taaatttatt agcatattag tttgcactat
      121 attttggaat tattttgaat atattatatt agaatgtaaa taacaactaa atatactaat
      181 ccaggtgtat caatataaga attaataaga aaatagatga aaataaaaga attataatta
      241 taatttagaa tttttaatgt taataaatag tgaatagaac taatatcatg gtaagataaa
      301 tctcgtcgct gagagacaca caaacaactc aacaaaataa attaaaagtt ttgaaaaaaa
      361 gttgttgaca aagtttcgaa gtgatgataa actaag
//
LOCUS       DAEVGI010000411          381 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEVGI010000411 DAEVGI010000000
VERSION     DAEVGI010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.415"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          176 a           33 c           47 g          125 t
ORIGIN      
        1 atgtagaatc aaataatttt aataaaataa ccttagttga ttatttaaac aatagcaaaa
       61 aagaggtatc aaaatatagc aataaaactc acaaacatat ttctagaatt aatagacttt
      121 actaagaatt tataatcatt acttaagaga tattactaaa gcttcaaaag ttaagttaaa
      181 gtcaaaagtt ttaatttgga tttttaaact tatacaatgg agctttgtga gattatattg
      241 ttaagaaaag aaaatacacc aattttaggt agcagtaata aatcaaaata tatgttaata
      301 aactcaaaga ggttaaaaaa tgtcttttta aagatagaaa attcttttaa agatgtaaac
      361 tattttgaaa aaaggatgaa a
//
LOCUS       DAEVGI010000412          363 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEVGI010000412 DAEVGI010000000
VERSION     DAEVGI010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.416"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          161 a           21 c           45 g          136 t
ORIGIN      
        1 taaattaaca aaatataaaa ctatagcaag gattggcttt atttataaat taataaattt
       61 gtaataattc ataagggggt tatatggcaa aattatatta tgaaaaaaat atatttagat
      121 ttaagtatat gagaatctat aagttttgtt tttgcaatta aatatatgaa ttcaaattaa
      181 atctaaagga cagtgatgtt aatgtaacta tagagtatgt aaaaatcttt ttaaaaggtt
      241 taaaatttta tattaggttt taaacttaga ttaatacact gaagaacatg acgattatat
      301 agttagtaaa atactatcaa ttaaatatta ataagaaaga tatttaaaaa tttcttgtta
      361 aaa
//
LOCUS       DAEVGI010000413          360 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEVGI010000413 DAEVGI010000000
VERSION     DAEVGI010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.417"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            80..>360
                     /locus_tag="K8M77_002698"
     rRNA            80..>360
                     /locus_tag="K8M77_002698"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          133 a           66 c           85 g           76 t
ORIGIN      
        1 atttaattta tagaaacaac aagccaaatt ggcaaaacca atttctattc tttgtaaaat
       61 gagaactata actaatatag gtcaagctac aaagggcgca tggtgaatgc cttggcatca
      121 ggagccgatg aaggacgtga taagctgcga taagcttcgg gtaggcgcac atagcctgag
      181 atccgaagat ctccgaatga ggaaactcac atgggaaacc ccatgtatca ttaagtgaat
      241 acatagctta atgaaggtaa cccagggaac tgaaacatct aagtacctgg aggaagagaa
      301 agaaaaatcg attttcttag tagcggcgag cgaaagggaa agagcccaaa ccggaaactt
//
LOCUS       DAEVGI010000414          359 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEVGI010000414 DAEVGI010000000
VERSION     DAEVGI010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.418"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          119 a           51 c           30 g          159 t
ORIGIN      
        1 gtcaccctca agcgacttcc ttagtttatc atcacttcga aatcttgtca acaacttttt
       61 tcaaaatatt ttttaatttt ttaattaaat ttaattttga aagtgaattg tttgtgtgtc
      121 tctcagcgac gagatttatc ttaccacata taaccccata aattaagctt tttatagtca
      181 tttatacaaa ttaaatatat ttttcttgtt ttttctaaat aattctttca aataaccata
      241 ttgatacact tggataagtt tgcttttatt cattgtctta ttatgtagaa aaaaactaca
      301 ttttcataaa tatcaaatta taaagcaaag tattaaattt attttttctt tattttaat
//
LOCUS       DAEVGI010000415          356 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEVGI010000415 DAEVGI010000000
VERSION     DAEVGI010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.419"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            32..316
                     /locus_tag="K8M77_002699"
                     /pseudo
     CDS             32..316
                     /locus_tag="K8M77_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
BASE COUNT          156 a           26 c           71 g          103 t
ORIGIN      
        1 tatatataca cagtgaaaat aggtgattta agtgaaaaat gaattaataa agatttctat
       61 aagaaactta gttgaatttg ttttaagaaa aggaagtata gatagtcgta taaaagcatc
      121 tgtaagagct ttagagggaa taaaaggaca caagaaaata cagagttctt attctgaaaa
      181 ggatagggct gaggttacct taaaagagga tattgatttt gaagatttta ctttaagggt
      241 agaaggtaga gcagatggaa tattagagga aaatgaaaaa ataattattg atgaaataaa
      301 aactactaca aaaaacgaat ctgttaagtt gttatgaaaa aatagcttaa ctattc
//
LOCUS       DAEVGI010000416          347 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEVGI010000416 DAEVGI010000000
VERSION     DAEVGI010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.420"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..347)
                     /locus_tag="K8M77_002700"
     CDS             complement(<1..347)
                     /locus_tag="K8M77_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003464379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087110.1"
                     /translation="MKRLLNKKLRKLDISSRELSELKLELLDTAEEMKKDFLNEGFSE
                     KEANEKVINTLQIDELINSTKESYLKANLINTRILSSLFLGIYTIFILICISNTTNGG
                     GYLSKGAYLPFKF"
BASE COUNT          128 a           46 c           34 g          139 t
ORIGIN      
        1 aaaaatttaa aaggtaaata tgcacctttt gataaatagc caccaccatt agtagtattt
       61 gaaatacaaa ttaaaataaa tatagtatat attcctagaa ataaagatga taaaatccta
      121 gtatttatta gatttgcttt aagataagac tctttagtag aatttataag ttcatctatc
      181 tgaagagtgt taattacttt ctcattagct tctttttcag aaaaaccttc atttaaaaaa
      241 tcctttttca tttcttctgc ggtatcaagt aactctaatt ttaattctga taattctcta
      301 gatgaaatat ctaatttcct taatttttta tttaaaagtc ttttcaa
//
LOCUS       DAEVGI010000417          334 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEVGI010000417 DAEVGI010000000
VERSION     DAEVGI010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.421"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>334
                     /locus_tag="K8M77_002701"
     CDS             <1..>334
                     /locus_tag="K8M77_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7087111.1"
                     /translation="KVDVDIYKEKGSELIGYLIAKGICKSVNKEISEDLPQNNKEQEN
                     INLQDNKTFKTNATAKVALDPRDNPSNNYRDLGEIFANERIKILPEICDLKTFLPATY
                     WKDSENKES"
BASE COUNT          148 a           33 c           52 g          101 t
ORIGIN      
        1 aaagttgatg tcgatattta taaagaaaaa ggctctgaat taataggtta tttaatagct
       61 aaaggaattt gtaagtctgt aaataaagaa attagtgaag atttacctca aaataataaa
      121 gaacaagaaa atattaattt acaagataat aaaaccttta aaactaatgc aactgcaaaa
      181 gttgctttag atcctaggga taatcctagt aataactata gagatttagg agaaattttt
      241 gcaaatgaaa gaataaaaat tctgcctgaa atatgtgatt taaaaacatt tttacctgca
      301 acttattgga aggattcgga aaataaagaa tcta
//
LOCUS       DAEVGI010000418          334 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEVGI010000418 DAEVGI010000000
VERSION     DAEVGI010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.422"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            51..>334
                     /locus_tag="K8M77_002702"
     CDS             51..>334
                     /locus_tag="K8M77_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBI7087112.1"
                     /translation="MRNALKLNKMNYIDNIHSRSKGWITRSVIDKKGYSQWHYKYAEL
                     KDLDMRDENIYITLNTFYKPCRRLENIKELNTLFIDLDYYKTGKTKDQVL"
BASE COUNT          144 a           31 c           63 g           96 t
ORIGIN      
        1 tagaaaatat gatataattt tcttagtttt acaaaggggg aggtgaataa gtgagaaacg
       61 cattaaagct gaataaaatg aactatatag ataatattca tagtagaagt aaaggatgga
      121 taaccaggag tgttatagat aaaaaaggat atagccaatg gcactataaa tacgctgaat
      181 taaaagattt agatatgagg gatgaaaata tctatataac tctaaatacc ttctataagc
      241 cttgtaggcg gttagaaaat ataaaagagt taaatacact gtttatagac ttggattatt
      301 ataaaactgg caaaactaaa gatcaggtat taat
//
LOCUS       DAEVGI010000419          332 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEVGI010000419 DAEVGI010000000
VERSION     DAEVGI010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.423"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            48..257
                     /locus_tag="K8M77_002703"
                     /pseudo
     CDS             48..257
                     /locus_tag="K8M77_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
BASE COUNT          134 a           36 c           58 g          104 t
ORIGIN      
        1 taaattgaat tcatcccttt atacatattt agaaagaaga tgagtttatg aggattattg
       61 ataatgaaaa agaatttaag gaattttgta atgaaaaagg gataagatta gcttatttaa
      121 gtactaataa atgttgtgct tgtagcgttt taaaacctaa aatagaggaa ttaataaaaa
      181 aataccataa tgcaaacatt atagaggtta aggctgataa aagtgtagac atagtatcac
      241 aattaagtat ttttatgcct ttggcaagaa cacccgtgat ttcaatcatg ggataaattg
      301 ccacgacata tgctaagagt ataaaccgtc ta
//
LOCUS       DAEVGI010000420          323 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEVGI010000420 DAEVGI010000000
VERSION     DAEVGI010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.424"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          140 a           19 c           36 g          128 t
ORIGIN      
        1 acttatcttt atatttttta aaaaaaatat aagttttata ttaataaata gaatattata
       61 acaaatatta tataaaaata taaaaaaata tatgatttaa tattaaaact tgattagatt
      121 tacttatgaa gtttttatgt aatgtttaac actaaattgc agttttgtta tatagaaaat
      181 tgcaattata attaaattat ctaagcaatt tattttaggg tgagaagttt atgaaagaag
      241 tattacactt agtagttgat ataggatcag ctactattaa ccatataata aatgaataag
      301 tcttttaaaa atatttaaag ggg
//
LOCUS       DAEVGI010000421          303 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEVGI010000421 DAEVGI010000000
VERSION     DAEVGI010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.425"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>303
                     /locus_tag="K8M77_002704"
     CDS             <1..>303
                     /locus_tag="K8M77_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBI7087113.1"
                     /translation="SELIGYLIAKGICRSVNKEISEDLYQNNEEQEIINLQNNKTFKT
                     NATAKVALDPRDNPSNNYRDLGEIFANEKIRILPEICDLKIFLPATYWKDSENKES"
BASE COUNT          133 a           30 c           46 g           94 t
ORIGIN      
        1 gttctgaatt aataggttat ttaatagcta aaggaatctg taggtctgtg aataaagaaa
       61 tcagtgaaga tttatatcaa aataatgaag aacaagaaat tattaattta caaaataata
      121 aaacctttaa aactaatgca actgcaaaag ttgctttaga tcctagagat aatcctagta
      181 acaactatag agatttagga gaaatttttg caaatgaaaa aataagaatt ttgcctgaaa
      241 tatgtgattt aaaaatattt ttacctgcaa cttattggaa agattcagaa aataaagaat
      301 ctg
//
LOCUS       DAEVGI010000422          301 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEVGI010000422 DAEVGI010000000
VERSION     DAEVGI010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.426"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          145 a           25 c           42 g           89 t
ORIGIN      
        1 aaaatatatt ataaaatgat acaatattct acagaaaagt atattgaatg taaattttgt
       61 ttaaaacact ttacctagtg tatctatata ggaaaaagta agtattttaa agaaaaatat
      121 agaaataata atatatttta tctaaaacag agagaagtat taaattaaat atggttaaaa
      181 tgataagata aatttcgtca gcaagagaca cggcaacaaa acatcaaata aaaatttaaa
      241 aaaagttgtt gacaaagttt cttaaagatg ttaaaataag aaagtcgctt gagggcgaca
      301 a
//
LOCUS       DAEVGI010000423          297 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEVGI010000423 DAEVGI010000000
VERSION     DAEVGI010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.427"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(219..293)
                     /locus_tag="K8M77_002705"
     tRNA            complement(219..293)
                     /locus_tag="K8M77_002705"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(258..260),aa:Glu,seq:ttc)
BASE COUNT          108 a           49 c           44 g           96 t
ORIGIN      
        1 ttgttattat attctacata aaaatacttt tattaacaag gttgaaatga agaagataca
       61 taacttcatt ttttggtgca caatttgtaa agaatgaaga ataatcactt aattaaaaat
      121 atacataaat tcattttatt atatccattt aagaaccttc tttttagctc aaattcactt
      181 tataaaaaaa actcgataag caatgcttat cgagttaatg gtgaccccta ggggaatcga
      241 acccctgtta ccaccgtgaa agggtggtgt cttaaccgct tgaccaaggg gccaaac
//
LOCUS       DAEVGI010000424          291 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEVGI010000424 DAEVGI010000000
VERSION     DAEVGI010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.428"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            1..268
                     /locus_tag="K8M77_002706"
                     /pseudo
     CDS             1..268
                     /locus_tag="K8M77_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          133 a           29 c           40 g           89 t
ORIGIN      
        1 tagaacaaaa atcgaagcta atgcaaaccg ttatacattt attttgaaga aatctacaaa
       61 taagtttgaa gagaaattac aaattaaagt tagaaaacta attgaaaata ttaataagga
      121 cttaaatatc acttttcata acgaaaagaa gatatctgta tcatatgcta ataatttgct
      181 aacttatatt attttattaa aggagaacac taatttagag tttgtttatg ggaaaggaaa
      241 aagaaaaagc aaacttcaaa aatatgctta agacttagaa aatttcatgg a
//
LOCUS       DAEVGI010000425          289 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEVGI010000425 DAEVGI010000000
VERSION     DAEVGI010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.429"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          111 a           39 c           31 g          108 t
ORIGIN      
        1 agatatttat tttttccata aaatcaacaa aaaaataaaa gagaggataa tatatcccct
       61 cctttatatt aaaggcctat ttaatgaaca ctcttttaaa ttaaaaacta ttgcgcgtta
      121 attttagatt ttaaagagtc tcctaatata tacgtctata tattagaaat ttttcttagt
      181 taactctcag cttataaact tttgcgctaa gtatataagt gagttttaac taagcataaa
      241 ttcccgttgt tcatcggtac taataaaaaa tttatttaga aagttaaaa
//
LOCUS       DAEVGI010000426          282 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEVGI010000426 DAEVGI010000000
VERSION     DAEVGI010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.430"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            96..171
                     /locus_tag="K8M77_002707"
     tRNA            96..171
                     /locus_tag="K8M77_002707"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Met,seq:cat)
     gene            175..251
                     /locus_tag="K8M77_002708"
     tRNA            175..251
                     /locus_tag="K8M77_002708"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:209..211,aa:Met,seq:cat)
BASE COUNT           68 a           76 c           77 g           61 t
ORIGIN      
        1 ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgatgct aacacatcgt
       61 accggttcga ttccggtctt cggcaccaat aatatcgcgg ggtggagcag ctggtagctc
      121 gtcgggctca taacccgaag gtcgtaggtt caagtcctgc ccccgcaacc acatggcggg
      181 atagctcagc tggctagagc attcggttca tacccgaagg gtcgcaagtt cgaatcttgt
      241 tcccgctacc atttaaactt aatatatgcc gaagtggcgg aa
//
LOCUS       DAEVGI010000427          275 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEVGI010000427 DAEVGI010000000
VERSION     DAEVGI010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.431"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>275
                     /locus_tag="K8M77_002709"
     CDS             <1..>275
                     /locus_tag="K8M77_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087114.1"
                     /translation="DKDDCKEHLKEEYKEKKDDCRGKDKEECKHHDKEEKHEEKCEED
                     KKYKDDKEDKYKKEDKYKDKDEVDDDCKDKHEEHKEYKDKDTDKDKH"
BASE COUNT          152 a           15 c           63 g           45 t
ORIGIN      
        1 ggataaagat gattgcaaag agcatttaaa agaagaatat aaagagaaaa aagatgactg
       61 tagaggaaaa gacaaagaag agtgcaagca ccatgataaa gaagagaagc atgaagaaaa
      121 atgtgaagag gataaaaaat ataaagatga taaagaagac aaatataaga aagaagataa
      181 atataaggat aaagacgaag tagatgatga ttgtaaagat aaacatgaag agcataaaga
      241 atataaggat aaagatacgg ataaagataa acatg
//
LOCUS       DAEVGI010000428          273 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEVGI010000428 DAEVGI010000000
VERSION     DAEVGI010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.432"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            32..>273
                     /locus_tag="K8M77_002710"
     CDS             32..>273
                     /locus_tag="K8M77_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBI7087115.1"
                     /translation="MNYVYILKCKDESLYTGWTNNLEKRIKAHNNGCGAKYTRARGPV
                     KLVYFESFENKREAQSREYYIKKLTRNQKLQLISSK"
BASE COUNT          117 a           23 c           54 g           79 t
ORIGIN      
        1 ggatgattta atattaaagg tggtgattac tttgaattat gtttatattt taaaatgtaa
       61 ggatgaatct ttatatactg gatggactaa taatttagaa aaaagaataa aagcgcataa
      121 taatggatgt ggagctaaat acacaagagc aagagggcca gtaaaacttg tttactttga
      181 atcttttgag aataaaagag aagctcaaag tagagagtat tacataaaga agcttacaag
      241 aaatcagaag ttacaattaa taagtagcaa aag
//
LOCUS       DAEVGI010000429          264 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEVGI010000429 DAEVGI010000000
VERSION     DAEVGI010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..264
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.433"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(169..244)
                     /locus_tag="K8M77_002711"
     tRNA            complement(169..244)
                     /locus_tag="K8M77_002711"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Lys,seq:ttt)
BASE COUNT           92 a           43 c           33 g           96 t
ORIGIN      
        1 tttactaata tataataaag tcgactaaaa ttctgttaaa tattaatata ttttgaactc
       61 tagtcgaatt tatataaaac tttattccat tattatttat tttactataa ttatttttat
      121 attaattttt atataaaaaa acctctctaa ctaattaaga gaggtttttg gtgatctgcc
      181 agggaatcga accctggaca ccgtgattaa aagtcacgtg ctctaccgac tgagctagca
      241 aatcataaga cctggcgacc acct
//
LOCUS       DAEVGI010000430          258 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.434, whole genome shotgun sequence.
ACCESSION   DAEVGI010000430 DAEVGI010000000
VERSION     DAEVGI010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.434"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>258
                     /locus_tag="K8M77_002712"
     CDS             <1..>258
                     /locus_tag="K8M77_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087116.1"
                     /translation="LNKRFFKLIESFNKGSLKNKILIDIENFIGHENIENKMEIELLY
                     ALDLFKEEYIKSIEELILNLKERLEENIYSNFEGDYGKLFS"
BASE COUNT          111 a           16 c           43 g           88 t
ORIGIN      
        1 ccttaaataa gagatttttt aagcttatag aatctttcaa caaggggtct ttaaaaaata
       61 aaattttaat tgatatagag aatttcatag gacatgaaaa tatagaaaat aaaatggaaa
      121 tagaactttt atacgcctta gatttattta aggaagagta tattaaaagt atagaggagt
      181 taattttaaa cttgaaagaa agattagagg aaaatatata ttctaatttt gaaggtgatt
      241 atggaaagtt attttcag
//
LOCUS       DAEVGI010000431          257 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEVGI010000431 DAEVGI010000000
VERSION     DAEVGI010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.435"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          124 a           26 c           35 g           72 t
ORIGIN      
        1 aatatctact ttattttaca tattaataca ataaattata tgaaaactta tcccagcgta
       61 tcaatataag aattagatag aaaagataag aaaaaagaag aattataaga ataataagtg
      121 aaatatggta tattataaaa gtgattaaat caatatacat ggtaagataa atctcgtcgc
      181 cgagggacac acaaacaact taacaaaata aataaaaagt tttgaaaaaa agttgttgac
      241 aaagtttcca agtgatg
//
LOCUS       DAEVGI010000432          246 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEVGI010000432 DAEVGI010000000
VERSION     DAEVGI010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.436"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..235
                     /locus_tag="K8M77_002713"
     rRNA            <1..235
                     /locus_tag="K8M77_002713"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           64 a           60 c           73 g           49 t
ORIGIN      
        1 tgtaggctga aactcgccta catgaagctg gagttactag taatcgcgaa tcagaatgtc
       61 gcggtgaata cgttcccggg tcttgtacac accgcccgtc acaccatgag agttggcaat
      121 acccgaagtc cgtgagctaa ccgcaaggag gcagcggccg aaggtagggt cagcgattgg
      181 ggtgaagtcg taacaaggta gccgtaggag aacctgcggc tggatcacct cctttctaag
      241 gaatac
//
LOCUS       DAEVGI010000433          227 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEVGI010000433 DAEVGI010000000
VERSION     DAEVGI010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.438"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            93..212
                     /locus_tag="K8M77_002714"
                     /pseudo
     CDS             93..212
                     /locus_tag="K8M77_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590400.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
BASE COUNT           74 a           33 c           47 g           73 t
ORIGIN      
        1 gaggtcggaa cgcccttgtt agcttggtta atctcatacc gttaggtgtg ttgcccaaga
       61 atcttggcat ttcaatgccg agtagttcaa attcaacatt taattcttta gaggatttag
      121 attcatatca aaagaatcct gaccatgtta aagcaggttc ttttgtaaaa aaagtagcat
      181 catcaagagt tgttgtagat tatgaaatgt aaaaaatagt taaggag
//
LOCUS       DAEVGI010000434          224 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEVGI010000434 DAEVGI010000000
VERSION     DAEVGI010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.439"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>224
                     /locus_tag="K8M77_002715"
     CDS             <1..>224
                     /locus_tag="K8M77_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBI7087117.1"
                     /translation="SKFSEVFKSITADNGSEFADLSEFELKTKTKVYFTHPYSSFEKG
                     TNERHNGLIRRFIPKGKRISDYSLETISFI"
BASE COUNT           82 a           34 c           36 g           72 t
ORIGIN      
        1 gcagtaaatt tagtgaagta tttaagagta taactgctga taatggctct gaatttgctg
       61 atttatcaga atttgaacta aaaacaaaaa ctaaagtgta tttcactcac ccatattctt
      121 catttgaaaa aggtacaaat gagcgtcata atggactgat taggcgtttt atcccgaaag
      181 gaaaacgaat ttcagattac tcacttgaaa ctatatcttt cata
//
LOCUS       DAEVGI010000435          215 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.442, whole genome shotgun sequence.
ACCESSION   DAEVGI010000435 DAEVGI010000000
VERSION     DAEVGI010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.442"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            complement(<1..>215)
                     /locus_tag="K8M77_002716"
     CDS             complement(<1..>215)
                     /locus_tag="K8M77_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI7087118.1"
                     /translation="IEYLNIKGMMKNKHLSKAIASQKFFEFKTKLTFKCKENHIELRI
                     VDRFYPSSKTCSNCGKVKKDLKLSDRI"
BASE COUNT           72 a           32 c           16 g           95 t
ORIGIN      
        1 taaattctat cagatagttt taaatctttc ttaacctttc cacaattact acaagtcttt
       61 gatgatggat aaaatctatc cactattcta agttctatat gattttcttt acatttaaat
      121 gttaacttag ttttaaattc aaaaaatttc tgacttgcaa tagctttaga taaatgctta
      181 tttttcatca ttcctttaat atttaaatat tcaat
//
LOCUS       DAEVGI010000436          211 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEVGI010000436 DAEVGI010000000
VERSION     DAEVGI010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.443"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..>211
                     /locus_tag="K8M77_002717"
     CDS             <1..>211
                     /locus_tag="K8M77_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HBI7087119.1"
                     /translation="NNRYYDYNRYYDDEYYQDDYYQNDYHCKEDYCCKDDCYLKINCN
                     CCKPGPRGPRGPQGEQGPQGERGFTG"
BASE COUNT           83 a           24 c           43 g           61 t
ORIGIN      
        1 ataataatag atattatgat tacaatagat attatgatga tgaatattat caagatgatt
       61 attatcaaaa tgattatcac tgcaaagaag actattgttg taaagatgat tgctatttaa
      121 aaataaattg taattgttgc aagcctggac caaggggacc aagaggccct caaggtgaac
      181 aaggacctca aggtgaaaga ggatttactg g
//
LOCUS       DAEVGI010000437          208 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.446, whole genome shotgun sequence.
ACCESSION   DAEVGI010000437 DAEVGI010000000
VERSION     DAEVGI010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.446"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT           78 a           16 c           16 g           98 t
ORIGIN      
        1 ttataaaatt aataaaaaaa cttgtaaaaa tagccctttt atgatagaat ttatttaaag
       61 gttgttaatt aattaacaaa gtttttccat gttcatttca ttttccttta agaacagttt
      121 ttactgttct ttttttatat aaaaagtatt taaaggttta atatatataa atacttttac
      181 ttaatgaatt ttattttaaa tattttta
//
LOCUS       DAEVGI010000438          205 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.denovo.447, whole genome shotgun sequence.
ACCESSION   DAEVGI010000438 DAEVGI010000000
VERSION     DAEVGI010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.denovo.447"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
BASE COUNT          104 a           20 c           27 g           54 t
ORIGIN      
        1 cgactttaaa atataaaaaa atacattcta gaatcttcaa ctgaaccaag tgtatctata
       61 gataaaaaaa agagaaaaag tatgaaatta gaagaaaaat aaaaataatt atgttaaatg
      121 ggaattatta tattaaaagg aatttataat tgtgatatca taaatatcgt catcaagaac
      181 aagcaagaaa cgcactacaa gatga
//
LOCUS       DAEVGI010000439         4902 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.guided.159, whole genome shotgun sequence.
ACCESSION   DAEVGI010000439 DAEVGI010000000
VERSION     DAEVGI010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.guided.159"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            71..712
                     /locus_tag="K8M77_002718"
     CDS             71..712
                     /locus_tag="K8M77_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBI7087120.1"
                     /translation="MDKNVMWLRECRINKVIKALEENNINGYLVNSKEELISKIEELT
                     NSGDIVACGGSMSLFEAGVIDHLRSGRYEFLDRYKEGLKPEDIKKIYRGAFSSDVYFT
                     STNAITEEGEIYNVDGNGNRVAAMLYGPDKVIIVAGYNKIVENLSEAIQRNRRISAPA
                     NAKRLNKKTPCAKVGNCMDCKSPEKICREYTLIRSQSNSNRIHVIFLNEELGY"
     gene            943..1314
                     /locus_tag="K8M77_002719"
     CDS             943..1314
                     /locus_tag="K8M77_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBI7087121.1"
                     /translation="MSKELIIAIIFMNLALLFYSIGVWSEKIQKTLKPWHLAFFYLGL
                     ICDTTGTGAMEKIAGGINFSFHTITGGLALILMLVHAIWATVVLVKKDKNMIAKFHKF
                     SIIVWVIWLIPFFTGAAMHML"
     gene            1560..2039
                     /gene="rlmH"
                     /locus_tag="K8M77_002720"
     CDS             1560..2039
                     /gene="rlmH"
                     /locus_tag="K8M77_002720"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBI7087122.1"
                     /translation="MNITVISVGKLKEKYLKQAIDEYSKRLSRYCKLEIIELPDEKTP
                     DNASEKEELQIKEKEGKLILSKIKDNMHVIAMDLKGNEITSEKFSKYIENCGVMGNSN
                     ITFVIGGSLGLSQEVIKRADYKLCFSKMTFPHQLFRVMLLEQVYRAFRIMKNEPYHK"
     gene            2775..4001
                     /gene="tetA(P)"
                     /locus_tag="K8M77_002721"
     CDS             2775..4001
                     /gene="tetA(P)"
                     /locus_tag="K8M77_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter TetA(P)"
                     /protein_id="HBI7087123.1"
                     /translation="MVNKLSAYKTYLLFSAITAMCFSLVATVMIVYHIEIVHLNPLQL
                     ILVGTTLELACFIFEIPTGIVADVYSRKLSIVIGGVLTGVGFILEGSISSFVFVLVAQ
                     IVWGLGSTFISGSLEAWIAEEEKNKDLDEIYIKGAQAGQIGAFIGIVLSTVIANFSVR
                     LPIIVSGVLFIILALFLWLYMPENNFKPSAPGDLNTFKKMVYTFKSGLKFVKSKSIIM
                     ILLAVTLFYGLSSEGYDRLSNAHFLQDTTLPKLGNLSSVTWFGIFGILGMILSFIVMH
                     FMAKNLKNEDNRKNGKLLLCINILYISSMLIFALTRNFSLMLIAYLATNTFRIINEPI
                     FSAWLNGHIDDNSRATVLSINGQMNSLGQILGGPIIGIIATNISVSIGIVCTSLLVAP
                     VLVLYIISMIMDKKIS"
     gene            4231..4560
                     /locus_tag="K8M77_002722"
     CDS             4231..4560
                     /locus_tag="K8M77_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087124.1"
                     /translation="MKKVYLKNQLKGYPIEVISNVLDVINVLGENYGANRDIEKDLGG
                     YIVIAENIVVIEMLKKDKLQGLIPEYTDIIECKEGVNWTSSLFLLSSDYAIVVVTTEE
                     LSKFLLE"
     gene            4578..4880
                     /locus_tag="K8M77_002723"
     CDS             4578..4880
                     /locus_tag="K8M77_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI7087125.1"
                     /translation="MSKNIKEDKIIKETKYCKIINQGKVGDDEYTYTIEKIYIKELKR
                     NEIRFCVYKSTRRGDETYIPRSLDVTELELMELIKKSIKEKVFSDEFIEMLKQELK"
BASE COUNT         1899 a          437 c          849 g         1717 t
ORIGIN      
        1 gactcgtaga acagggaatt aaacaagatt tataggtatt tatagatttt tggcaacggg
       61 agtttatatt atggataaga atgttatgtg gttaagagaa tgtagaatta ataaagttat
      121 aaaagcttta gaagaaaata atataaatgg ctatttagta aattcaaaag aagaacttat
      181 aagtaagata gaagaattaa ctaatagtgg agatatagta gcttgcggag gctctatgtc
      241 cttatttgaa gctggagtta tagatcatct tagaagtgga agatatgaat ttttagatag
      301 atataaagag gggttaaaac ctgaagatat aaaaaagata tacagaggtg ccttttcatc
      361 agatgtatat ttcacaagta caaatgcaat tactgaagag ggtgaaatat ataatgttga
      421 tggtaatgga aacagagtag cagcaatgct ttatggacca gataaggtca taattgtagc
      481 aggatataat aaaattgttg aaaatttaag tgaggctata caaagaaata gaaggatttc
      541 agctcctgct aatgcaaaaa gattaaataa gaaaacacct tgtgctaagg tgggaaattg
      601 catggattgt aaaagtccag aaaagatatg tagagaatat actttaatca gaagtcaaag
      661 taattcaaat agaatacatg taatattttt aaatgaagaa ttaggatatt aaaatttaca
      721 agtaaggaca tgtaaaaatg ttcttacttt tttttataaa tctgaatttg ataaagaatg
      781 ataatttgta aataattatt attaaatgta ttgctttgca attattaaat tttgaaaatt
      841 aataaagcct ttttattaag aaattaatat tactaaattg taagtagaat acaatggtat
      901 tataatatga acatggttca aataaaaaag gagatgaaag tgatgtcaaa agaattaatt
      961 atagcaataa tatttatgaa tttagcacta ttattttata gtataggtgt ttggtctgaa
     1021 aagattcaaa aaactttaaa accatggcac ttagcatttt tctatttagg tctaatttgt
     1081 gatactacag gaactggagc tatggaaaag atagctggag gaataaactt tagtttccat
     1141 acaataacag gtggattagc attaatatta atgttagtac atgctatctg ggcaactgta
     1201 gttttagtaa agaaagataa aaatatgata gctaaattcc acaaatttag tataattgtt
     1261 tgggtaattt ggttaatacc attctttact ggagctgcaa tgcatatgct ataaaatttt
     1321 taaaagacta tgtaagaata tatcttgcat agtctttttt atatataaaa attaatattt
     1381 tagttttaaa atattatagt atttaaaatt tattaagacc atgtactaaa aattaataac
     1441 tcatttatat atctcactaa ttataaatta tcaataagag gtatagggta ttttatagga
     1501 ttactaagag ctttacttta tggtaatatt attggtagtt aaaactagga gggcgaaaga
     1561 tgaatataac agttatttca gtagggaaac taaaggaaaa gtatttaaag caagctatag
     1621 atgagtattc aaaaagatta agtagatatt gtaaattaga aataatagaa cttcctgatg
     1681 agaaaacacc agataatgct tcagaaaaag aggagcttca aataaaagaa aaagaaggta
     1741 agttaatact ttcaaaaata aaggataata tgcatgtaat tgctatggat ttaaaaggta
     1801 atgaaataac ttctgagaaa ttttcaaagt atatagagaa ttgtggagtt atgggaaatt
     1861 caaatattac ctttgtaatt ggaggaagcc ttggtttatc acaagaagta ataaaaagag
     1921 ctgattacaa attatgcttt tcaaaaatga catttccaca tcaattattt agagtaatgt
     1981 tactagaaca agtttataga gcctttagaa taatgaagaa cgaaccgtac cataaataaa
     2041 tgaggttttg gatagaattg gatatataaa tggacgatag gtgtgcttta atttttgcaa
     2101 tgaaacttac aaaaatctaa agtttgaatt tttgttgggt attttaattt tatttataga
     2161 ttcttcattt ttcactactt gagaaaatta gaatttgttt ttcagaatct gtagcattga
     2221 aaaaattttt aaaattatat atactataat tatactaaaa tataagcata aaaaatataa
     2281 atgtaaaggt gttgtaaata tgcgcaaaga ggtaattttt aattaaatta actgaataag
     2341 gattattttt aaaagtatat gaaaaatctt gaataactca aggtttattt gtggtagaat
     2401 caattattga taatattgat attaccatgt ttatttgtgt actctaaaaa taattagtat
     2461 tataatgagc atgttacatc tttatttgca ggataggaaa accgtgttct aaacacggta
     2521 tttttatgtc tatttttgct atatatagat atggtataag attttaattt gaatagctta
     2581 atcataagtt atataattaa agttctatat tttatgttta tatttaggtt atatgattat
     2641 gaggctgtaa gtgaattatg ttcatttaca gctttttttg tttaaggaga ttattttgat
     2701 gatttatata tttaaatatg aagattatga aaaacaaaca aattttttaa gaaatactag
     2761 gatggtgatg attaatggtt aataaacttt cagcatataa aacttattta ttattttcag
     2821 ctattacagc aatgtgtttt tcgttagtag ctacagttat gatagtgtat cacattgaaa
     2881 tagttcattt aaatccactt cagcttatac ttgttggaac tactttggaa ttagcatgct
     2941 ttatatttga aattcctaca ggtatagttg cagatgtgta tagtcgtaaa ctatctattg
     3001 ttattggggg agttttaaca ggagtgggat ttattttaga aggttctatt tctagttttg
     3061 ttttcgtact tgtagcacag attgtatggg gattagggtc tacttttatc agtggctcgc
     3121 ttgaagcttg gattgcggaa gaagagaaga ataaagattt agatgaaatt tatataaagg
     3181 gagcacaagc agggcagata ggagcattta ttggaatagt actaagcact gtaatagcta
     3241 atttctctgt aaggcttcct attatagtta gtggagtttt atttataatt cttgcattat
     3301 ttttatggtt atatatgcca gaaaataatt ttaaaccatc tgctcctggg gatttaaata
     3361 cattcaaaaa gatggtatat acatttaaat ctggtcttaa atttgtaaaa agtaaatcta
     3421 taattatgat tttacttgca gtaactttat tttatggatt atcaagtgaa ggttatgata
     3481 gactttctaa tgcgcatttt ttacaagata ctacacttcc taaacttgga aaccttagtt
     3541 cagtgacttg gtttggaatt tttggaattt taggaatgat attgagcttc atagtaatgc
     3601 attttatggc aaagaatctt aagaatgagg ataataggaa aaatggaaaa ctattattat
     3661 gcataaatat actttatata tcgtctatgt tgatatttgc tcttacaaga aactttagtt
     3721 taatgttaat agcttatttg gcaacaaata cctttagaat tataaatgaa cctatattca
     3781 gtgcgtggtt aaatgggcat atagatgata attctagagc tactgtgctt tctataaatg
     3841 gacaaatgaa ttccttaggt caaattttag gtggaccgat tataggaatc atagctacaa
     3901 atatttcagt aagtattggt atagtatgta cttcattatt agtagcacca gtattagtgt
     3961 tatatattat ttctatgata atggataaaa aaatatcatg aatataaggg attaaaaatg
     4021 aagataattt gattgaacaa tacaagaagg ttcgtaaatt ttttgtagaa atcttcaaaa
     4081 acacttttaa attacaatat tagaattagg ttaattactg tattaaaaga taaattaaat
     4141 atatattatt aacttagaaa aacaaacttt tacttgtatt tatgtataca agtaaaagtt
     4201 ttttactttc ttagaaggga gaaatttata atgaaaaagg tttatttaaa aaatcaatta
     4261 aaaggatatc ctatagaagt aatcagtaat gttttagacg tcattaatgt attaggtgaa
     4321 aattatggag ctaatagaga tatagagaaa gatttaggag gatatatagt tatagcagaa
     4381 aatatcgttg ttatcgaaat gttaaagaaa gataagttac aaggtttaat accagaatat
     4441 actgatatta ttgaatgtaa ggaaggagtt aattggactt cttccttatt tttgctttca
     4501 agtgattatg caattgtagt agtaacaaca gaagaacttt ctaagttcct attagaataa
     4561 atggaggtta atttaatgtg agtaagaata taaaagagga taagataatc aaggaaacta
     4621 agtattgtaa gataataaat caaggaaaag ttggagatga tgaatataca tatacaatag
     4681 aaaagatata tatcaaggaa ctaaaaagga atgaaataag attctgtgta tataaatcta
     4741 ctagaagagg tgatgagact tatattccaa gaagtcttga tgtaactgaa ttagagttaa
     4801 tggagttaat aaagaagtcc attaaagaga aagtattttc agatgaattc atagaaatgt
     4861 tgaagcaaga attaaaatag ctacttaatt acaagaaaag ta
//
LOCUS       DAEVGI010000440         4172 bp    DNA     linear   BCT 03-SEP-2021
DEFINITION  TPA_asm: Clostridium perfringens strain PH127 isolate PH127
            SAMEA5717899-rid12930323.guided.175, whole genome shotgun sequence.
ACCESSION   DAEVGI010000440 DAEVGI010000000
VERSION     DAEVGI010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5717899
            Sequence Read Archive: ERR3377464
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridium perfringens
  ORGANISM  Clostridium perfringens
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001122506.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001122506.1
            Genome Coverage       :: 65x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/03/2021 02:46:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,727
            CDSs (total)                      :: 2,688
            Genes (coding)                    :: 2,597
            CDSs (with protein)               :: 2,597
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 29
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 30 of 91
            Pseudo Genes (incomplete)         :: 55 of 91
            Pseudo Genes (internal stop)      :: 26 of 91
            Pseudo Genes (multiple problems)  :: 16 of 91
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Clostridium perfringens"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5717899-rid12930323.guided.175"
                     /strain="PH127"
                     /isolate="PH127"
                     /isolation_source="Human gut (gastroenteritis)"
                     /host="Homo sapiens"
                     /db_xref="taxon:1502"
                     /country="United Kingdom"
                     /collection_date="2016"
     gene            <1..1102
                     /locus_tag="K8M77_002724"
     CDS             <1..1102
                     /locus_tag="K8M77_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI7087126.1"
                     /translation="NNKSKYVIDYLNRLNHDLENITLFLERKVDCINEEKDDLNKPSM
                     INNFTDKEYAFLGECTELIRLLEKNINNLYEYRREKSRKRIKVKFNLKELLIGNSSLK
                     VKDLRVAYSLKLAIAVSLIMFIVDLFKIHQGRWIVTSVYVVIQPYEEETLTKAIKRFK
                     GTIIGVIIYISIFTFFPHIIPLELLLLILMFLYFTQKDYDKKVVCTALMALSFGLSRS
                     TVGYLAFYRFFFVIIGIVIALGVNKLIFPQSIKNSIYDLKERYLELTSKLLCELKSIL
                     YEEGYNGNTVKLLLDCNLIESKLIENKLIAENLELKDLVDKQSIILSKIRCLVLFINY
                     SNWGISSKHINVDKNLLNVIFNKIEEELREIY"
     gene            1406..1915
                     /locus_tag="K8M77_002725"
     CDS             1406..1915
                     /locus_tag="K8M77_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI7087127.1"
                     /translation="MDFITITNYFAEYGLLFLFVIILLEYMNLPGLPAGIIMPAVGVL
                     VSRSEMSLITALLVSVIAGLIGSWILYCIGRFGGELILQKYLNKFPSHESFVNRKIDY
                     LREKGYTGILISKLIPMARTVISIPAGVLKLNFLKYSAYATLGIFIWNTVLISSGYFL
                     GEELLKFLG"
     gene            2232..2936
                     /locus_tag="K8M77_002726"
     CDS             2232..2936
                     /locus_tag="K8M77_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI7087128.1"
                     /translation="MEKYNVLVVEDEKEIAEAIEIYLKNQGYNVFKGSNGLEGLEIVE
                     KEEIHLAVVDIMMPKMDGATMVMKIRESYDFPIIMLSAKSEDMDKILGLNIGADDYVT
                     KPFNPLELLARVNSQLRRYSKYLNMVKKDEKKTNSYAIGGLEVNSDRKEVILDGDVVK
                     VTPIEFKILQLLIKSPGRVFSAEEIYERVWNENAVNTDTVMVHVRNIREKIEIDPKNP
                     KYLKVVWGVGYKIEKQ"
     gene            2911..>4172
                     /locus_tag="K8M77_002727"
     CDS             2911..>4172
                     /locus_tag="K8M77_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003480154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBI7087129.1"
                     /translation="MAIKSKSNKENLQVNKENRTNKTKWIFTIITLILIGIISVCLLL
                     SYPIIKKNTPNYITSYGLFNNYNFREKIIDTNYGIYYDYLEKKAEENEKEMNPMEDLF
                     TLKNENRYEDKESYEYSLKEDTRLLNERVKSLINGLHDLGNLRVYAMDEEGNTVYETK
                     GDQNWLLEALAKGSYIENGDLENYYRFYMVLKYNNRGKVSLEKIYGEDSHRVSNLLLD
                     MDSQYFAYQNEYEAKPIENMTYVYAVPKTLEYQDNLYYSEMDNKIMALGSASAPFIMI
                     GMIVTCLLALLIPFKYTREVLGFNKFLKIPFEINFIIASIMLAFTIESGRLIIIPTLQ
                     GGLSEALKMTSLPHLDIVTNIINVLYWMVIYGFVFFEVIYVKYIFNIGFKNYIRKHLL
                     VYKLKDIIFVILRKLIKYIKGIDLNKNS"
BASE COUNT         1719 a          366 c          710 g         1377 t
ORIGIN      
        1 taataataaa tctaaatacg taatagacta tttaaataga ttaaatcatg atttggaaaa
       61 cataacttta tttttagaaa gaaaagtgga ttgtattaat gaggaaaagg atgatttaaa
      121 taagccttct atgataaata attttactga taaagaatat gcttttttag gggaatgcac
      181 tgaacttata agacttttag agaaaaatat aaataattta tatgaatata ggagggagaa
      241 atcaaggaag agaattaaag taaaatttaa tcttaaggaa ctactaatag gaaatagttc
      301 tttaaaagtg aaagatttaa gggtagccta ttctttaaaa ctagccatag cagtttccct
      361 aataatgttt atagtagatt tatttaaaat acatcaggga agatggattg ttactagtgt
      421 ttatgtggtt atacagccct atgaagaaga aaccttaaca aaagcaataa aaagattcaa
      481 agggacaata ataggagtga taatctatat ttctatattt acatttttcc cacatattat
      541 tcctttagaa ttgcttctat taattttaat gtttctttac tttactcaaa aggattatga
      601 taaaaaggtt gtatgcacag cacttatggc tctaagcttt ggattatcta gaagtacagt
      661 tggatactta gctttttata gatttttctt tgtaataata gggatagtaa tagctttagg
      721 agttaataag cttatttttc cacagagcat aaaaaattct atatacgatt taaaggaaag
      781 gtatttagaa ttaacaagta aacttctttg tgagttaaag agcatattat atgaagaggg
      841 atataatgga aatacagtta aacttctatt agattgtaat cttattgagt ctaagttaat
      901 agaaaataaa ttaattgctg aaaatttaga acttaaagac ttagtagata aacagagcat
      961 aattttgagt aagataaggt gtcttgtatt atttataaat tactcaaatt ggggaatttc
     1021 atcaaaacac attaatgtag ataaaaattt attaaatgta atttttaata aaatagagga
     1081 agaactaagg gagatttatt aggaataaaa gatcacataa aatgatgtta cttaaaagtt
     1141 tagaagttaa gaattaagtt aaattaagat tactaactaa aatcaaaaaa atattaagaa
     1201 actctttaga aaatctttag atttatctga ggagtttctt tatttttaaa gtttattata
     1261 agagtaacaa atgagaagaa agcaagtaac aatttagata atagaaatgc tttttagaat
     1321 taagttagtt taaaagcatt ttagaattaa caattaataa aaatatatag gtcaaataaa
     1381 attttaaaat tgaaaggtaa ggggtatgga ttttataact ataacaaact attttgcaga
     1441 gtacgggctt ttatttttat ttgtgattat tcttttagaa tatatgaatt taccagggct
     1501 tcctgcaggg ataataatgc cagcagtggg cgtgttagtt tcaagaagtg aaatgagtct
     1561 tataacagca cttttggtat ctgtaatagc aggtctaata ggaagttgga tactttattg
     1621 cataggaaga tttggaggag aattaattct tcaaaaatat ctaaataaat ttccaagtca
     1681 cgaaagcttt gtaaatagaa aaattgatta tttaagagaa aagggttata caggaatttt
     1741 gataagcaaa ctaattccaa tggctaggac agtaatatca ataccagcag gagttttgaa
     1801 attaaatttt cttaagtata gtgcttatgc aactttagga atttttatat ggaatacagt
     1861 tttaatatca tcaggatatt tcttaggaga ggaattatta aaatttttag gatagaaata
     1921 aggaggtgtt ttttggaaat atagacaatg aggtattaac aaactttaaa gaatataagg
     1981 aaatagttaa ggaaaggcta tgtttctaac agtgaaaaag ctttctatga tattattata
     2041 ttgaaattat tggataaaat ttaaaggatt agagaaatat tttagtttaa gcttataaaa
     2101 ttagatagtc tttaagagag agctaaaatc aacactaatt aacttatagc taaatttaaa
     2161 tatagaatta acctaagttt ttatatggta tctaataaaa atatagtaat aaaaaaggga
     2221 gtaataaaga gatggaaaag tataatgttt tagttgttga agacgaaaag gaaatagcag
     2281 aggctattga aatatatcta aaaaatcaag gatacaatgt atttaaagga agtaatggtt
     2341 tagaaggatt agaaatagtt gaaaaagaag agattcatct agctgttgtt gatataatga
     2401 tgcctaaaat ggatggagca acaatggtaa tgaaaattag agaaagttat gactttccta
     2461 taataatgtt atcagcaaaa tcagaggaca tggataaaat tttaggactt aatattggag
     2521 ccgatgatta tgtaacaaaa ccatttaatc cactagagct tcttgcaagg gttaattctc
     2581 aattaagaag atattctaaa tacttaaaca tggtaaaaaa ggatgagaag aaaacaaatt
     2641 cttatgccat aggtggatta gaagttaata gtgatagaaa agaagtaata ttagatggag
     2701 atgtagtaaa agtaactcct atagaattta aaatacttca attacttatt aaaagtccag
     2761 gaagagtttt ttcagcagaa gaaatatatg aaagagtatg gaatgaaaat gcagtaaaca
     2821 cagacactgt aatggtccat gtaagaaata taagagaaaa aatagagata gatccaaaaa
     2881 atccaaaata tttaaaggtg gtgtggggag ttggctataa aatcgaaaag caataaggaa
     2941 aacttacaag ttaataagga aaatagaact aataaaacaa agtggatttt cacaataata
     3001 accttaatat taataggaat tatatctgta tgtttattat tatcatatcc tataataaag
     3061 aaaaatactc caaactatat tacaagttat gggttattta ataattataa ttttagggaa
     3121 aaaataattg atacaaacta tggaatttac tatgattatt tagaaaaaaa agctgaggaa
     3181 aatgaaaaag aaatgaatcc catggaggat ctatttactt taaaaaatga aaatagatat
     3241 gaagataaag agtcttatga atatagttta aaagaagata ctagattatt aaatgaacgt
     3301 gttaaaagtc ttattaatgg attacatgat ttaggaaatc taagagtata tgctatggat
     3361 gaagaaggaa atacagtata tgaaaccaaa ggagatcaaa attggttgct tgaagcttta
     3421 gcaaagggtt catatattga aaatggtgat ttagaaaatt attatagatt ttatatggta
     3481 cttaagtata acaatagagg aaaggtttct ttagaaaaaa tttatggaga ggatagccat
     3541 agggtaagca atttattatt agatatggat tcacagtatt ttgcatatca aaatgagtat
     3601 gaagcaaaac caatagagaa tatgacctat gtgtatgcag taccaaaaac tttagaatat
     3661 caagataact tatattatag tgaaatggat aataagatta tggcacttgg gagtgctagc
     3721 gctcctttta tcatgattgg aatgatagtc acttgtttac ttgctttact tattccattt
     3781 aaatatacaa gagaggtttt aggttttaat aagtttttaa agattccttt tgagattaac
     3841 tttataatag cttcaattat gctagcattc acaattgaaa gtggaagact tataataatt
     3901 ccaacacttc aaggaggatt aagtgaagcc ctaaaaatga ctagcttacc acatttagat
     3961 atagttacta atataattaa tgtgctttat tggatggtca tatatggctt tgtattcttt
     4021 gaagtaatct atgtaaaata tatatttaat attggattta aaaattatat aagaaagcat
     4081 ttattagttt ataaattaaa agacattata tttgttatct taagaaaatt aataaaatat
     4141 attaaaggaa tagatttaaa taaaaatagt ga
//
